LOCUS       DAJTYH010000001        94483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTYH010000001 DAJTYH010000000
VERSION     DAJTYH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 94483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..991)
                     /gene="icd"
                     /locus_tag="OO609_000001"
     CDS             complement(<1..991)
                     /gene="icd"
                     /locus_tag="OO609_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6631570.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGD"
     gene            1163..1816
                     /gene="rluE"
                     /locus_tag="OO609_000002"
     CDS             1163..1816
                     /gene="rluE"
                     /locus_tag="OO609_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6631571.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            1826..2287
                     /gene="nudJ"
                     /locus_tag="OO609_000003"
     CDS             1826..2287
                     /gene="nudJ"
                     /locus_tag="OO609_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6631572.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2341..3447
                     /gene="mnmA"
                     /locus_tag="OO609_000004"
     CDS             2341..3447
                     /gene="mnmA"
                     /locus_tag="OO609_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6631573.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3483..4124
                     /gene="hflD"
                     /locus_tag="OO609_000005"
     CDS             3483..4124
                     /gene="hflD"
                     /locus_tag="OO609_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6631574.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4128..5498
                     /gene="purB"
                     /locus_tag="OO609_000006"
     CDS             4128..5498
                     /gene="purB"
                     /locus_tag="OO609_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6631575.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5667..6338
                     /gene="phoP"
                     /locus_tag="OO609_000007"
     CDS             5667..6338
                     /gene="phoP"
                     /locus_tag="OO609_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6631576.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6338..7798
                     /gene="phoQ"
                     /locus_tag="OO609_000008"
     CDS             6338..7798
                     /gene="phoQ"
                     /locus_tag="OO609_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6631577.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8595..8876)
                     /locus_tag="OO609_000009"
     CDS             complement(8595..8876)
                     /locus_tag="OO609_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631578.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(8890..10551)
                     /locus_tag="OO609_000010"
     CDS             complement(8890..10551)
                     /locus_tag="OO609_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6631579.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10535..10861)
                     /locus_tag="OO609_000011"
     CDS             complement(10535..10861)
                     /locus_tag="OO609_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6631580.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(10917..11099)
                     /locus_tag="OO609_000012"
     CDS             complement(10917..11099)
                     /locus_tag="OO609_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631581.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11083..11523)
                     /locus_tag="OO609_000013"
     CDS             complement(11083..11523)
                     /locus_tag="OO609_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6631582.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11523..11819)
                     /locus_tag="OO609_000014"
     CDS             complement(11523..11819)
                     /locus_tag="OO609_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6631583.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(11816..12154)
                     /locus_tag="OO609_000015"
     CDS             complement(11816..12154)
                     /locus_tag="OO609_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6631584.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12151..13362)
                     /locus_tag="OO609_000016"
     CDS             complement(12151..13362)
                     /locus_tag="OO609_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6631585.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13364..13936)
                     /locus_tag="OO609_000017"
     CDS             complement(13364..13936)
                     /locus_tag="OO609_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6631586.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(13976..15133)
                     /locus_tag="OO609_000018"
                     /pseudo
     CDS             complement(13976..15133)
                     /locus_tag="OO609_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15437..15598)
                     /locus_tag="OO609_000019"
     CDS             complement(15437..15598)
                     /locus_tag="OO609_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6631587.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(15608..15913)
                     /locus_tag="OO609_000020"
     CDS             complement(15608..15913)
                     /locus_tag="OO609_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631588.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(15910..16188)
                     /locus_tag="OO609_000021"
     CDS             complement(15910..16188)
                     /locus_tag="OO609_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631589.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16477..18225)
                     /locus_tag="OO609_000022"
     CDS             complement(16477..18225)
                     /locus_tag="OO609_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6631590.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18222..18521)
                     /locus_tag="OO609_000023"
     CDS             complement(18222..18521)
                     /locus_tag="OO609_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6631591.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18527..18760)
                     /locus_tag="OO609_000024"
     CDS             complement(18527..18760)
                     /locus_tag="OO609_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631592.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(18762..18982)
                     /locus_tag="OO609_000025"
                     /pseudo
     CDS             complement(18762..18982)
                     /locus_tag="OO609_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18975..19196)
                     /locus_tag="OO609_000026"
     CDS             complement(18975..19196)
                     /locus_tag="OO609_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631593.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19186..19386)
                     /locus_tag="OO609_000027"
     CDS             complement(19186..19386)
                     /locus_tag="OO609_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6631594.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19516..19695
                     /locus_tag="OO609_000028"
     CDS             19516..19695
                     /locus_tag="OO609_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631595.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            19820..20149
                     /locus_tag="OO609_000029"
     CDS             19820..20149
                     /locus_tag="OO609_000029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631596.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20199..20387)
                     /locus_tag="OO609_000030"
     CDS             complement(20199..20387)
                     /locus_tag="OO609_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6631597.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(20762..21991)
                     /locus_tag="OO609_000031"
     CDS             complement(20762..21991)
                     /locus_tag="OO609_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6631598.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22240..23361
                     /gene="roxA"
                     /locus_tag="OO609_000032"
                     /gene_synonym="ycfD"
     CDS             22240..23361
                     /gene="roxA"
                     /locus_tag="OO609_000032"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6631599.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23410..24636)
                     /gene="pepT"
                     /locus_tag="OO609_000033"
     CDS             complement(23410..24636)
                     /gene="pepT"
                     /locus_tag="OO609_000033"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6631600.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            24886..26022
                     /gene="potA"
                     /locus_tag="OO609_000034"
     CDS             24886..26022
                     /gene="potA"
                     /locus_tag="OO609_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6631601.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26006..26863
                     /gene="potB"
                     /locus_tag="OO609_000035"
     CDS             26006..26863
                     /gene="potB"
                     /locus_tag="OO609_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6631602.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            26860..27654
                     /gene="potC"
                     /locus_tag="OO609_000036"
     CDS             26860..27654
                     /gene="potC"
                     /locus_tag="OO609_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6631603.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27651..28697
                     /gene="potD"
                     /locus_tag="OO609_000037"
     CDS             27651..28697
                     /gene="potD"
                     /locus_tag="OO609_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6631604.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(28853..29674)
                     /gene="cobB"
                     /locus_tag="OO609_000038"
     CDS             complement(28853..29674)
                     /gene="cobB"
                     /locus_tag="OO609_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6631605.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(29690..30601)
                     /gene="nagK"
                     /locus_tag="OO609_000039"
     CDS             complement(29690..30601)
                     /gene="nagK"
                     /locus_tag="OO609_000039"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6631606.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30630..31874)
                     /gene="lolE"
                     /locus_tag="OO609_000040"
     CDS             complement(30630..31874)
                     /gene="lolE"
                     /locus_tag="OO609_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6631607.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(31874..32575)
                     /gene="lolD"
                     /locus_tag="OO609_000041"
     CDS             complement(31874..32575)
                     /gene="lolD"
                     /locus_tag="OO609_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6631608.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32568..33767)
                     /gene="lolC"
                     /locus_tag="OO609_000042"
     CDS             complement(32568..33767)
                     /gene="lolC"
                     /locus_tag="OO609_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6631609.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34029..35102
                     /locus_tag="OO609_000043"
     CDS             34029..35102
                     /locus_tag="OO609_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6631610.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35230..38676
                     /gene="mfd"
                     /locus_tag="OO609_000044"
     CDS             35230..38676
                     /gene="mfd"
                     /locus_tag="OO609_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6631611.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            38819..39781
                     /gene="ldtC"
                     /locus_tag="OO609_000045"
     CDS             38819..39781
                     /gene="ldtC"
                     /locus_tag="OO609_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6631612.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(39864..40076)
                     /locus_tag="OO609_000046"
     CDS             complement(39864..40076)
                     /locus_tag="OO609_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6631613.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40361..40993
                     /gene="comR"
                     /locus_tag="OO609_000047"
     CDS             40361..40993
                     /gene="comR"
                     /locus_tag="OO609_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6631614.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41055..41594)
                     /locus_tag="OO609_000048"
     CDS             complement(41055..41594)
                     /locus_tag="OO609_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6631615.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(41821..43125)
                     /gene="ndh"
                     /locus_tag="OO609_000049"
     CDS             complement(41821..43125)
                     /gene="ndh"
                     /locus_tag="OO609_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6631616.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43533..44075)
                     /locus_tag="OO609_000050"
     CDS             complement(43533..44075)
                     /locus_tag="OO609_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6631617.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44098..45123)
                     /gene="nagZ"
                     /locus_tag="OO609_000051"
     CDS             complement(44098..45123)
                     /gene="nagZ"
                     /locus_tag="OO609_000051"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6631618.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45134..45958)
                     /gene="thiK"
                     /locus_tag="OO609_000052"
     CDS             complement(45134..45958)
                     /gene="thiK"
                     /locus_tag="OO609_000052"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6631619.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(45939..46580)
                     /gene="lpoB"
                     /locus_tag="OO609_000053"
     CDS             complement(45939..46580)
                     /gene="lpoB"
                     /locus_tag="OO609_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6631620.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46595..46972)
                     /locus_tag="OO609_000054"
     CDS             complement(46595..46972)
                     /locus_tag="OO609_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6631621.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(46975..47334)
                     /gene="hinT"
                     /locus_tag="OO609_000055"
     CDS             complement(46975..47334)
                     /gene="hinT"
                     /locus_tag="OO609_000055"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6631622.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            47667..49852
                     /gene="fhuE"
                     /locus_tag="OO609_000056"
                     /pseudo
     CDS             47667..49852
                     /gene="fhuE"
                     /locus_tag="OO609_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(49912..51345)
                     /gene="ptsG"
                     /locus_tag="OO609_000057"
     CDS             complement(49912..51345)
                     /gene="ptsG"
                     /locus_tag="OO609_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6631623.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(51640..52437)
                     /locus_tag="OO609_000058"
     CDS             complement(51640..52437)
                     /locus_tag="OO609_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6631624.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52448..53452)
                     /gene="holB"
                     /locus_tag="OO609_000059"
     CDS             complement(52448..53452)
                     /gene="holB"
                     /locus_tag="OO609_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6631625.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53449..54090)
                     /locus_tag="OO609_000060"
     CDS             complement(53449..54090)
                     /locus_tag="OO609_000060"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6631626.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54080..55102)
                     /gene="yceG"
                     /locus_tag="OO609_000061"
     CDS             complement(54080..55102)
                     /gene="yceG"
                     /locus_tag="OO609_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6631627.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55105..55914)
                     /gene="pabC"
                     /locus_tag="OO609_000062"
     CDS             complement(55105..55914)
                     /gene="pabC"
                     /locus_tag="OO609_000062"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6631628.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56034..57275)
                     /gene="fabF"
                     /locus_tag="OO609_000063"
     CDS             complement(56034..57275)
                     /gene="fabF"
                     /locus_tag="OO609_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6631629.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57362..57598)
                     /gene="acpP"
                     /locus_tag="OO609_000064"
     CDS             complement(57362..57598)
                     /gene="acpP"
                     /locus_tag="OO609_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6631630.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(57809..58543)
                     /gene="fabG"
                     /locus_tag="OO609_000065"
     CDS             complement(57809..58543)
                     /gene="fabG"
                     /locus_tag="OO609_000065"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6631631.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58556..59485)
                     /gene="fabD"
                     /locus_tag="OO609_000066"
     CDS             complement(58556..59485)
                     /gene="fabD"
                     /locus_tag="OO609_000066"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6631632.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59501..60454)
                     /gene="fabH"
                     /locus_tag="OO609_000067"
     CDS             complement(59501..60454)
                     /gene="fabH"
                     /locus_tag="OO609_000067"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6631633.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60522..61592)
                     /gene="plsX"
                     /locus_tag="OO609_000068"
     CDS             complement(60522..61592)
                     /gene="plsX"
                     /locus_tag="OO609_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6631634.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(61673..61846)
                     /gene="rpmF"
                     /locus_tag="OO609_000069"
     CDS             complement(61673..61846)
                     /gene="rpmF"
                     /locus_tag="OO609_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6631635.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(61898..62419)
                     /gene="yceD"
                     /locus_tag="OO609_000070"
     CDS             complement(61898..62419)
                     /gene="yceD"
                     /locus_tag="OO609_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6631636.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            62618..63202
                     /gene="yceF"
                     /locus_tag="OO609_000071"
     CDS             62618..63202
                     /gene="yceF"
                     /locus_tag="OO609_000071"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6631637.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63314..64273)
                     /gene="rluC"
                     /locus_tag="OO609_000072"
     CDS             complement(63314..64273)
                     /gene="rluC"
                     /locus_tag="OO609_000072"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6631638.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            64846..68037
                     /gene="rne"
                     /locus_tag="OO609_000073"
     CDS             64846..68037
                     /gene="rne"
                     /locus_tag="OO609_000073"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6631639.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68234..69176)
                     /gene="flgL"
                     /locus_tag="OO609_000074"
                     /pseudo
     CDS             complement(68234..69176)
                     /gene="flgL"
                     /locus_tag="OO609_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69188..70830)
                     /gene="flgK"
                     /locus_tag="OO609_000075"
                     /pseudo
     CDS             complement(69188..70830)
                     /gene="flgK"
                     /locus_tag="OO609_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(70896..71837)
                     /gene="flgJ"
                     /locus_tag="OO609_000076"
     CDS             complement(70896..71837)
                     /gene="flgJ"
                     /locus_tag="OO609_000076"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6631640.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(71837..72934)
                     /gene="flgI"
                     /locus_tag="OO609_000077"
     CDS             complement(71837..72934)
                     /gene="flgI"
                     /locus_tag="OO609_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6631641.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(72946..73644)
                     /gene="flgH"
                     /locus_tag="OO609_000078"
     CDS             complement(72946..73644)
                     /gene="flgH"
                     /locus_tag="OO609_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6631642.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(73697..74479)
                     /gene="flgG"
                     /locus_tag="OO609_000079"
     CDS             complement(73697..74479)
                     /gene="flgG"
                     /locus_tag="OO609_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6631643.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(74617..75368)
                     /gene="flgF"
                     /locus_tag="OO609_000080"
                     /pseudo
     CDS             complement(74617..75368)
                     /gene="flgF"
                     /locus_tag="OO609_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75388..76527)
                     /locus_tag="OO609_000081"
     CDS             complement(75388..76527)
                     /locus_tag="OO609_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6631644.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(76552..77247)
                     /gene="flgD"
                     /locus_tag="OO609_000082"
     CDS             complement(76552..77247)
                     /gene="flgD"
                     /locus_tag="OO609_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6631645.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77259..77662)
                     /gene="flgC"
                     /locus_tag="OO609_000083"
                     /pseudo
     CDS             complement(77259..77662)
                     /gene="flgC"
                     /locus_tag="OO609_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(77666..78082)
                     /gene="flgB"
                     /locus_tag="OO609_000084"
     CDS             complement(77666..78082)
                     /gene="flgB"
                     /locus_tag="OO609_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6631646.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78237..78896
                     /gene="flgA"
                     /locus_tag="OO609_000085"
     CDS             78237..78896
                     /gene="flgA"
                     /locus_tag="OO609_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6631647.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            78972..79265
                     /gene="flgM"
                     /locus_tag="OO609_000086"
     CDS             78972..79265
                     /gene="flgM"
                     /locus_tag="OO609_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6631648.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79270..79686
                     /gene="flgN"
                     /locus_tag="OO609_000087"
     CDS             79270..79686
                     /gene="flgN"
                     /locus_tag="OO609_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6631649.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(79726..81261)
                     /gene="murJ"
                     /locus_tag="OO609_000088"
     CDS             complement(79726..81261)
                     /gene="murJ"
                     /locus_tag="OO609_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6631650.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81461..82294)
                     /locus_tag="OO609_000089"
     CDS             complement(81461..82294)
                     /locus_tag="OO609_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6631651.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWCIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLCD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82296..82943)
                     /locus_tag="OO609_000090"
     CDS             complement(82296..82943)
                     /locus_tag="OO609_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6631652.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(82954..83538)
                     /gene="rimJ"
                     /locus_tag="OO609_000091"
     CDS             complement(82954..83538)
                     /gene="rimJ"
                     /locus_tag="OO609_000091"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6631653.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            83774..84982
                     /gene="mdtH"
                     /locus_tag="OO609_000092"
     CDS             83774..84982
                     /gene="mdtH"
                     /locus_tag="OO609_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6631654.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85046..85693
                     /gene="grxB"
                     /locus_tag="OO609_000093"
     CDS             85046..85693
                     /gene="grxB"
                     /locus_tag="OO609_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6631655.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            85827..86387
                     /locus_tag="OO609_000094"
     CDS             85827..86387
                     /locus_tag="OO609_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6631656.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86493..87539
                     /gene="pyrC"
                     /locus_tag="OO609_000095"
     CDS             86493..87539
                     /gene="pyrC"
                     /locus_tag="OO609_000095"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6631657.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            87613..87858
                     /gene="dinI"
                     /locus_tag="OO609_000096"
     CDS             87613..87858
                     /gene="dinI"
                     /locus_tag="OO609_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6631658.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88148..88402
                     /gene="bssS"
                     /locus_tag="OO609_000097"
     CDS             88148..88402
                     /gene="bssS"
                     /locus_tag="OO609_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6631659.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88517..89635
                     /gene="solA"
                     /locus_tag="OO609_000098"
     CDS             88517..89635
                     /gene="solA"
                     /locus_tag="OO609_000098"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6631660.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            89683..89796
                     /locus_tag="OO609_000099"
     CDS             89683..89796
                     /locus_tag="OO609_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6631661.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90057..90623
                     /locus_tag="OO609_000100"
     CDS             90057..90623
                     /locus_tag="OO609_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6631662.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            90627..91202
                     /locus_tag="OO609_000101"
     CDS             90627..91202
                     /locus_tag="OO609_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6631663.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91244..92296)
                     /locus_tag="OO609_000102"
     CDS             complement(91244..92296)
                     /locus_tag="OO609_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6631664.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            92521..93441
                     /gene="lpxL"
                     /locus_tag="OO609_000103"
     CDS             92521..93441
                     /gene="lpxL"
                     /locus_tag="OO609_000103"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6631665.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            93613..93810
                     /locus_tag="OO609_000104"
     CDS             93613..93810
                     /locus_tag="OO609_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6631666.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            93834..>94483
                     /locus_tag="OO609_000105"
     CDS             93834..>94483
                     /locus_tag="OO609_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6631667.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTL"
BASE COUNT        22713 a        25072 c        23338 g        23360 t
ORIGIN      
        1 cgtcaccgat gtttgctcca ggggcgatac cgataccgcc aacctgcgct gccagagcgt
       61 cagaaatgta gtcaccgttc aggttcatac aggcgataac atcatattca gccggacgca
      121 gcaggatctg ttgcaggaat gcatcagcaa tcacgtcttt aatgacgatc tctttgccgg
      181 tattcgggtt tttaactttc agccacgggc cgccgtcgat cagttcaccg ccaaattctt
      241 cacgcgccag ctggtagccc cagtctttaa acgcgccttc ggtgaacttc atgatgttgc
      301 ctttgtgcac cagggtcaca gagtcacgat cgttagcaat tgcgtattcg atcgctgcac
      361 gaaccagacg tttggtgcct tcttcagaac acggcttaat accgataccg caatgttccg
      421 ggaagcgaat tttcttcacg cccatctctt cacgcaggaa tttaatcact ttctcggcgt
      481 cggcagagtc agctttccat tcgatacccg cataaatgtc ttccgagttt tcacggaaga
      541 taaccatatc ggtcagttca gggtgtttaa ccgggcttgg agtgccctga tagtaacgta
      601 ccggacgcag gcagatgtag agatccagtt cctgacgcag ggcaacgttg agagaacgaa
      661 taccgccacc aaccggagtg gtcagcgggc ctttaatggc aacgcgatat tcacgaatca
      721 gatcaagggt ttcagcaggc agccagacat cctgaccata aacctgtgtg gatttttcac
      781 cggtgtaaat ttccatccag gagattttac gctcgccttt ataggctttc tcgactgcag
      841 cgtcgaccac tttcagcatg gctggggtta catctacacc gattccatca ccttcaatgt
      901 aagggataat cggattttca ggaacgttga gtttgccgtt ttgcagggtg atcttcttgc
      961 cttgtgccgg aacaactact ttactttcca ttcacctctc cttcgagcgc tactggtttg
     1021 ctcgtctgcc accacgttgc atatgcgttt gcgtcctgcg atacggatgc tttagagcaa
     1081 ttttttgtta atgatttgta attgacttgt caatactacc ctattgtttg ccgtcatgaa
     1141 agatgcgcgt tattaggcta taatgcggca attcataatc tctgaaaata ccatgcaaaa
     1201 aacttctttt agaaatcacc aggttaagcg attcagctcg caacgttcta ccaggcgtaa
     1261 acatgaaaac cagcccacgc gtgtgatcct cttcaataaa ccctacgatg ttcttccgca
     1321 gttcaccgat gaaaccggac gcaaaacatt aaaagaattc atcccggttc agggcgttta
     1381 tgcagcaggt cgccttgacc gcgatagcga agggttgctg gtgctgacca ataacggcgc
     1441 gttgcaggcg cgtttaaccc agccgggtaa acgcaccgga aaaatctatt atgtgcaggt
     1501 ggaaggtatt cccacacaag acgcacttga agccttgcgc aatggcgtaa ccttaaatga
     1561 tggccctacc ctgcccgcag gcgcggagct ggttgacgaa cccgcgtggt tatggccgcg
     1621 gaatccacca attcgtgaac gcaaaagtat tcccaccagc tggctgaaga tcaccttata
     1681 tgaaggacgt aatcgccagg tgcgccgcat gaccgcccat gttggtttcc ccacgctgcg
     1741 actgattcgc tatgcgatgg gagattactc tttggataat cttgccaatg gcgaatggcg
     1801 agaagtgaca gattaaggat gcaaaatgtt taaaccgcac gttaccgttg cttgcgtggt
     1861 gcacgcagaa ggcaaatttt tagtcgttga agagacgatt aatggtaaag cgttatggaa
     1921 ccaacctgcc gggcatctgg aagccgatga aaccttagtg gaagccgccg cccgtgaact
     1981 gtgggaagaa accggcatca gcgcgcagcc gcaacacttt attcgtatgc atcagtggat
     2041 tgcgccagat aaaacgccgt ttttgcgttt cctctttgcc attgagcttg agcaaatatg
     2101 cccgacccag cctcatgaca gcgatatcga ctgctgccgt tgggtcagcg ccgaagaaat
     2161 tttacaggcg tcaaatcttc gttcgccgct ggtggcggaa agtattcgtt gttatcaaag
     2221 cgggcaacgt tatccgctgg agatgattgg cgattttaac tggcctttta caaagggtgt
     2281 catctaaaag gctgcgtgat agaatacgcc gccttgaagt tcaatgtcgt gagtgatcca
     2341 atgtctgaaa ccgcaaaaaa agtaatcgtc ggcatgtccg gcggtgtcga ttcctccgtt
     2401 tctgcctggc tgttgcaaca acagggatat caggtcgaag gcctgtttat gaagaactgg
     2461 gaagaagacg acggtgagga atattgcaca gcagcagcgg atctggctga tgcccaggcg
     2521 gtctgcgaca agctcggcat tgaactgcac accgttaact ttgctgccga gtactgggac
     2581 aacgtcttcg aactgttcct tgccgaatat aaagccggtc gcacgccgaa tccggatatt
     2641 ctgtgcaaca aggagatcaa atttaaagcc ttcctcgaat ttgccgccga agatttaggt
     2701 gccgattata tcgctaccgg tcattacgta cgtcgggccg atgtcgatgg caagagccgc
     2761 ctgctgcgtg gtctggacag caataaagac cagagctact tcctttatac gctcagccat
     2821 gagcagattg cgcaaagcct gttcccggta ggcgaactgg aaaaaccgca ggtgcgtaag
     2881 attgctgaag atcttggtct ggtcaccgcg aagaaaaaag actctaccgg catttgcttc
     2941 attggcgaac gtaaattccg cgagttcctg ggccgttatc tcccggcgca accgggcaaa
     3001 atcattaccg tcgatggcga tgaaattggt gagcaccagg ggctgatgta tcacactctc
     3061 ggtcagcgta aaggtctggg tatcggtggc accaaagaag gtaccgaaga accgtggtat
     3121 gtggtggaca aagacgtcga aaacaacatt ctggttgtcg ctcagggcca tgaacacccg
     3181 cggctgatgt ctgtcgggtt gattgcccag cagctgcact gggtcgatcg cgaaccattc
     3241 accggcacta tgcgttgcac ggtaaaaacc cgctatcgcc agaccgacat tccgtgcacc
     3301 gtcaaggcgc tggacgacga tcgcattaaa gtgattttcg atgagccggt tgccgccgtg
     3361 acgccgggcc agtctgccgt cttctataac ggtgaagtgt gcctcggtgg cggtattatt
     3421 gagcagcgtc tgccgctgcc ggtctgatta ttatctttac ttaaaacaag gaagcagtga
     3481 acgtggcaaa gaattactat gacatcaccc tcgccctggc cggtatttgt cagtcggcac
     3541 gcctggtgca acaactcgct caccaggggc attgtgatgc cgatgcgcta cacgtctcac
     3601 tcaacagtat tattgatatg aaccccagct cgacgctggc ggtttttggc ggtagcgaag
     3661 ccaacctgcg cgtcgggctg gaaaccctgc tcggcgtgct caatgccagc agttgccagg
     3721 gcttaaacgc cgaattaacc cgctacacac tcagcttgat ggtgcttgag cgcaaactct
     3781 cctcagcgaa aggcgcgctc gacactctgg gcaaccggat caacggcctg caacgccagc
     3841 tcgaacactt cgatttacag tccgaaacgc tgatgagcgc gatggctgct atctatgttg
     3901 atgtgatcag cccgcttggc ccgcgcattc aggtcaccgg ttcccctgct gtactgcaaa
     3961 gcccacaagt gcaggcgaaa gttcgcgcaa ccctgctggc aggcattcgc gccgccgtgc
     4021 tctggcacca ggtcggcggc ggacgtctac aactgatgtt ttctcgtaat cgcctgacca
     4081 ctcaggcaaa acaaattctt gctcatttaa ccccggagtt gtgatctatg gaattatcct
     4141 cactgaccgc cgtttcccct gtcgatggac gctacggcga taaagtcagc gcgctgcgcg
     4201 ggattttcag cgaatatggt ttgctgaaat tccgtgtaca agttgaagta cgttggctgc
     4261 aaaaactggc cgcgcacgca gcgatcaagg aagttcctgc ttttgctgcc gacgcaatcg
     4321 gttaccttga tgcaattgtc gccaatttca gcgaagaaga tgccgcacgc atcaaaacca
     4381 tcgagcgtac cactaaccac gacgttaaag cggttgagta tttcctgaaa gaaaaagtgg
     4441 cggagatccc ggaactgcac gcggtttctg aattcatcca ctttgcctgt acttcggaag
     4501 atatcaataa cctctcccac gcattaatgc tgaaaaccgc gcgtgatgaa gtgatcctgc
     4561 cgtactggcg tcaactgatt gatggcatta aagatctcgc cgttcagtac cgcgatatcc
     4621 cgctgctgtc tcgtacccac ggtcagccag ccacgccgtc aaccatcggt aaagagatgg
     4681 caaacgtcgc ctaccgtatg gagcgccagc accgccagct taaccaggtg gagatcctcg
     4741 gcaaaatcaa cggcgcggtc ggtaactata acgcccacat cgccgcttac ccggaagttg
     4801 actggcatca gttcagcgaa gagttcgtca cctcgctggg tattcaatgg aacccgtaca
     4861 ctacccagat tgaaccgcac gactacattg ccgaactgtt tgattgcgtt gcgcgcttca
     4921 acaccattct gatcgacttt gaccgtgacg tctggggtta tatcgccctt aaccacttca
     4981 aacagaaaac cattgctggt gagattggtt cttccaccat gccgcataaa gttaacccga
     5041 tcgacttcga aaactccgaa gggaatctgg gcctttccaa cgcggtattg cagcatctgg
     5101 caagcaaact gccggtttcc cgctggcagc gtgacctgac cgactccacc gtgctgcgta
     5161 acctcggcgt gggtatcggt tatgcgctga ttgcgtatca atccaccctg aaaggcgtga
     5221 gcaaactgga agtgaaccgt gaccatctgc tggatgagct ggatcacaac tgggaagtgc
     5281 tggcagaacc aatccagaca gttatgcgtc gctatggcat cgaaaaaccg tacgagaagc
     5341 tgaaagagct tactcgcggt aagcgcgttg acgccgaagg catgaagcag tttatcgatg
     5401 gtctggcgtt gccagaagaa gagaaagccc gcctgaaagc gatgacgccg gctaactata
     5461 ttggtcgagc tatcacgatg gttgatgagc tgaaataagc ctcaaatcag tgccggatgg
     5521 cgatgctgtc cggcctgctt attaagatta tccacttttt cttttttcac tttacctccc
     5581 ctccccgctg gtttatttaa tgtttacccc cataaacaca taatcgcgtt acactatttt
     5641 aataattaag acagggagaa ataaaaatgc gcgtactggt tgttgaagac aatgcgttgt
     5701 tacgtcacca ccttaaagtt cagattcagg atgctggtca tcaggtcgat gacgcagaag
     5761 atgccaaaga agccgattat tatctcaatg aacatttacc ggatattgcg attgtcgatc
     5821 tcggattgcc tgacgaggac ggtctgtcac tgattcgccg ctggcgtagc aacgatgttt
     5881 cactgccgat tctggtatta accgcccgtg aaagctggca ggacaaagtc gaagtattaa
     5941 gtgccggtgc tgatgattac gtgactaaac cgtttcatat tgaagaggtg atggcgcgaa
     6001 tgcaggcatt aatgcggcgt aatagcggtc tggcttcaca ggtcatttcg ctccccccgt
     6061 ttcaggttga tctctctcgc cgtgaattat ctattaatga cgaagtgatc aaactgaccg
     6121 cgttcgaata caccattatg gaaacgttga tacgcaataa tggcaaagtg gtcagcaaag
     6181 attcgttaat gctacaactc tatccggatg cggagctgcg ggaaagccat accattgatg
     6241 tactgatggg acgtctgcgc aaaaaaattc aggcacaata tccgcaagaa gtcattacca
     6301 ccgttcgcgg ccagggctac ctgttcgaat tgcgctgatg aaaaaattac tgcgtctttt
     6361 tttcccgctc tcgctgcggg tacgttttct gttggcaacg gcagcggtag tactggtgct
     6421 ttcgcttgcc tacggaatgg tcgcgctgat cggttatagc gtcagtttcg ataaaactac
     6481 gtttcggctg ttacgtggcg agagcaatct gttctatacc cttgcgcagt gggaaaacaa
     6541 taagttgcat gtcgagttac ccgaaaatat cgacaagcaa agccccacca tgacgctaat
     6601 ttatgatgag aacgggcagc ttttatgggc gcaacgtgac gtgccctggc tgatgaaaat
     6661 gatccagcct gactggctga aatcgaatgg ttttcatgaa attgaagcgg atgttaacga
     6721 taccagcctc ttgctgagtg gagatcattc gatacagcaa cagttgcagg aagtgcggga
     6781 agatgatgac gacgcggaga tgacccactc ggtggcggta aacgtctacc cggcaacatc
     6841 gcggatgcca aaattaacca ttgtggtggt ggataccatt ccggtggagc taaaaagttc
     6901 ctatatggtc tggagctggt ttatctatgt gctctcagcc aatctgctgt tagtgatccc
     6961 gctgctgtgg gtcgccgcct ggtggagttt acgccccatc gaagccctgg caaaagaagt
     7021 ccgcgaactg gaagaacata accgcgaatt gctcaatcca gccacaacgc gagaactgac
     7081 cagtctggta cgaaacctga accgattgtt aaaaagtgaa cgcgaacgtt acgacaaata
     7141 ccgtacgacg ctcaccgacc tgacccatag tctgaaaacg ccactggcgg tgctgcaaag
     7201 tacgctgcgt tctctgcgta gtgaaaagat gagcgtcagt gatgctgagc cggtaatgct
     7261 ggagcaaatc agccgcattt cacagcaaat tggctactac ctgcatcgtg ccagtatgcg
     7321 cggcgggaca ttgctcagcc gcgagctgca tccggtcgcc ccactgctgg acaatctcac
     7381 ctcagcgctg aacaaagtgt atcaacgcaa aggggtcaat atctctctcg atatttcgcc
     7441 agagatcagc tttgtcggtg agcagaacga ttttgtcgag gtgatgggca acgtgctgga
     7501 taatgcctgt aaatattgcc tcgagtttgt cgaaatttct gcaaggcaaa ccgacgagca
     7561 tctctatatt gtggtcgagg atgatggccc cggtattcca ttaagcaagc gagaggtcat
     7621 tttcgaccgt ggtcaacggg ttgatacttt acgccctggg caaggtgtag ggctggcggt
     7681 agcccgcgaa atcaccgagc aatatgaggg taaaatcgtc gccggagaga gcatgctggg
     7741 cggtgcgcgg atggaggtga tttttggtcg ccagcattct gcgccgaaag atgaataaat
     7801 atgtcctttt accactacat caagtcattg attcgatcaa acaattttca ttgaaaaagt
     7861 gccaaaaatc gcaaattgac tacaccatta actacaccgt tcggtgcact gtatgaaaca
     7921 acgtggaaca aatagacaca agaaatatac aggcgggtaa tctttccagg gggaagcgca
     7981 ccaattcatg aggggcgtta atgtcgatat ggggatcccc ataacggggc taccggattt
     8041 tttttccggt taacctttaa tcaggctggt gggttttaca tacccttgat caccgtaatg
     8101 atgatccaga tgtgcacctt ttccaccaat ggcggaaaag ctccaaccgt aacgatgatc
     8161 gctatggttg cctcgatggt ctgcgtggtc attatgaccg ttcagccagt gaccttaatc
     8221 agaatttttc gctaaaaaat gacgttggcc acgtcatccg gaaagcacaa aatcacgtcg
     8281 atttttaatg aacggttaaa ccgtcttttt gtcacggtgt gtcactggtt gtcactctcc
     8341 gtcacgattg aggtggtggt cattctgccc gaccgcgcat ctttgcgcta tcgggaatat
     8401 caaacagtta cctcgccgac ttcctcaaat tgaggtttcc gaaaatatca gagcgggtta
     8461 gcgattaagc tgacgagtaa ttaaactgcg aaaatttcgt agtttctgaa tatattccgg
     8521 cctgctggtt aacagtcctc agatgtgagg gatgtagcca gcgcaatttt gcgctttgct
     8581 ttaatctcag tgagttaccg ccgtaaccgc tccggcttct tccagtggta cgtgattttc
     8641 tccttctccc gatacagttc aacgcggcga ttgtaggcca gcatttccag aacgcggatc
     8701 cgtatgtcgc gcatatccgc atcattaagc tggataccat cacggcgcat cacctcagca
     8761 acaacacgcg cataattttc ggctgtcacg ctgtccggct gcgtggtctg ttcgtcagcc
     8821 tgctggctga ttccagagac gcggcggatt aatcgcagta tttcggcttc tgtcatgctg
     8881 ctgacctcat tacaccccgc taaattcttc cagtttctgg cggtggctgt cgcttatatc
     8941 aaaagcaaaa tcctcatgct ctgcctggaa tgtaccaaac gccatcagcg ccgctacgct
     9001 cgggtctatc ttgttcggtg attttttctt gttcggcttg atattggcgt tcgcgtcact
     9061 ctgcatcacg acgttactca tcgaccagga caacaccgga tcgccacgat gcacaatcac
     9121 cctgcggtta acaaaaactt cgaacgattt cgccgccgga ctgaatctga ggtaggtttg
     9181 cgggaacggc tccacctcaa aaccagcccc ctgtaattgc gtcctgagat gcgtggcgtt
     9241 ccacgtatca aagcccacca gcctgatatt aaatttctcc gcatcctgca tgatgtcatc
     9301 tctgatccgg tcgtaatcaa tgcagtcacc tggcgttgtg cgtatccagc ccgctttagc
     9361 ccactggcga tagacagcgc ggtttttatt ggcggggttc tgtagctgga actccggcag
     9421 ataatgacgg gaaatcagca taatggtttt accgaccgga aaggcatagc atacgctgga
     9481 aatatcgctg gttgatgata agtccagccc cgcgtaacac tcctgaccgt gtaaatcttc
     9541 ctccgtgaac gttccggcac actcagccca tgcaccatta cccatccacg gggtagtccc
     9601 ctgacaccag atattaaatc gctttgtcat catctccacc cactgcgacg gaataccccg
     9661 cgctttctgg atggttgagg ccagtttttc acgatccacg gaaacatcaa tattagggtt
     9721 cgcctttatc cacatcgccg gatcatcaac ctcgttttcg tcgtccagtt cgtaaatcag
     9781 tacaaaaatt gaatcgttaa cctcttcgcc gtccaggatc tggcagcaat aatcatagtg
     9841 ctgtttacag gctgaaacga cgttactgcc cgatgtggta atggcaaata acagcccttc
     9901 gggacgtgcg cccatcccca gctcaagcgc ggaataaacg ccgttatcgg ggtgtaggtg
     9961 gtattcgtcc acgatggaaa gactggggtt tgtcccctcg atggttgccg cttttgctgc
    10021 cagcggcttt aacaggctgt tgcttttcgg gtgtatcacc ttatgcgcct gaatattcac
    10081 ccgcctgcgt aacggtcggg ataaaaggca catctgacgc gcattatcaa acacgatccg
    10141 cgcctggtca cggctcaccg ctgcggtgta gatatcctgc tgaccgttct ccataatcag
    10201 aaaccagtta gccagaatcg cggcggtcgt ggatttcgca tttttgcgcg gcacttcgat
    10261 aaaggcgctc gtgtatttgc gccgtccggt ggccttaacc ttaaagccga ggatgcacgc
    10321 aaaggcgaac tgctgccacg gctccagctc aatgggtctg ccacgcatcg gtccttttac
    10381 gtgcgggcac accctggaaa aggcaataaa ccgctccaca acctcacgat cgaacgtgta
    10441 aagggggctt ttaaggtccg aaaagtaccg tttaacagcc tgttttaacc gtttacaggc
    10501 cggaattttg cccgttttta cgtcttctgc gtacttattc caggcggtca agctcgtcct
    10561 cttcttctgt ttccggtgga tttttacggc ggcttatcgg gtcaaaaccg agcaaggagg
    10621 ccatttttat catcactctt tcagcgtcgg attttgcgct taatgcgggg tttctgctct
    10681 cgccgccctg actgttaaca atgctgaacc cgcgcgccgc aaggtctgca acggctttcc
    10741 ggtaaatgga gtagttgacg caatacagtt ccagattgct ccagtcggcg ggggtcaggt
    10801 ctccccgttc cgcaagctgc cgcgattttt cccgccactg cttcacggcg atatcatcca
    10861 ggtaggcggg gtcgggtttg tcctgaaaac gccccccacc ccgtcataca gcctcatcag
    10921 cgattgcgga aacattccat aacctcgcgg tcacggtcgg ttaatcgctt cgctgtgatg
    10981 cgttctgcgc gtcctgacgc tttatcttta tgccctgttt cctgtgtctt ccatgcgtca
    11041 cgctgcctta taagtccacg gataaggcgg ttttgttccc gctcattcat cagcgtcata
    11101 catccagtta ttgcggttag cagcccgttc ttcctcttca cgaaatccac ctgcggcacg
    11161 cttgcttttt gttgccggat caagccattt cgttttctgg ttatgacacg cctggcacaa
    11221 tggttgatgg ttccattcgg gccagaagag aacatcatca ccgccattaa tcgggataat
    11281 gtgatccacc accacggcgg gcgtatatat ccccttctca aggcatcgca cgcataacgg
    11341 gtttttactc agatacatgg cgcggtattt gtcccactgt ctggaatatc cacgcgcgcg
    11401 gcggtgtcct cgtctggcat cctctgcacg ccatgccgcc cgcctgtgct cctcacactt
    11461 gccggattta acgcgcctgt tacagcctgg ttctgtgcat cgtcttaatg gttgccacgg
    11521 catcagtacc cccccacatc acggtaagcc gtccagagtg cgcctatcgt catgggtacg
    11581 cgtgtttttg cgttatccgc gacaatctgg cgattttcat acaggtgagc gataaacatc
    11641 atgcagccaa tctttatggc tggcgtgaac gccagcccgt catcaaagcg cctgcctatg
    11701 tgtatctggc acgcctcaag cgatgcggca atgtatccgc tgattaactg gtcttcatcg
    11761 tcgccatcga tgcggcaatg gagtttcact tcttccaggg tgataagttc ttctgtcatt
    11821 tttccgcgcc ctcacgacaa agaatttcaa ggcgtgtcct ggcggcatcc ggcagcggct
    11881 gcccgatgat attcagtaca cgccccgcca gcggccccgt actgaccttt atccggctgg
    11941 tggcgttgat gtccttccgg taccgtatcc agatccttac ggtaccaatc gccagctctg
    12001 cgcccgatga aatggcctcc ttgctgctga ccatgttcac gcttgcccag agtgtgtgac
    12061 cgtcctccca cgtttcgagt atttcgccgg ttatatccct ggtctgtttc agggtctgaa
    12121 tcgttatcct gtcacgcagt cgccctatgt tcattccggt ttttctccct cgctgatttt
    12181 tacttcctgt ttccatgcct ggctgaactc atcaccacca tcacgcggtg acagcccttc
    12241 acgttcgcgg gcttcgttcg gacacataac gtcggatttt atgccgcgct cataggtggc
    12301 aaagcgttcg ccaggtgtgg cccgtaacag gtccgcgctg tcaaactcca cctgataccg
    12361 gataccaggt acaggcgagg ccaccagcaa ggcgtttttt atctgctgct caaagttcgc
    12421 cagccacggg cgcatcgtca tggtgagaaa tgcgcggctt gcctcgctga agttgctgta
    12481 ggtgctgttg ctgtattcct gcaaaaaaat cggcgagacg ttgaacattc gggcgatgtc
    12541 ttcaatggtg aagcgacggg aggccagcca ttcggcatcc tgattactca tgcccagctg
    12601 ctgatagctc atcccccctt caaggatggg cgtttttccg gcgtttctgg cccctttgta
    12661 gcgttccagt gcggctaatg cctgtttgcc cttcacgccg tccagccatt cgcctgacgt
    12721 gataacccct gccgccatca tgccatcacg catcacgctc gcgccgtggc gttgttgggc
    12781 cagcccaagc cccagcgatt cgcggcagat ggttacaggt gagcgcccca taaaaccgtc
    12841 atccgtggag taacgaaggt ggagaacctc ccacggtaaa tagttgcgga tgtttccggt
    12901 gtaggcgtca gtgatgcagt agcgccagtt atgatcacct gtctgctcca cgttcaccga
    12961 ctgcggcggg taaggatgta aagccgccgg aaaaccatca cgcccccact gaataaccgc
    13021 ataagcatta ccacttaaca ggcagtagcg gatcatcatt cgcttaaact ggtagggtgt
    13081 ctgccacgcg ttcgggcgct cgttgaggat gtgatcgacc ggatgagaat caagccattc
    13141 gcgggcctcc ttccccttct cattgcgtac caggtacagg taacacggca tggtagccac
    13201 cgcgtcagag atgaccgtga cggcgttcat gacggcgggg agtgattccg ctgtcccaga
    13261 tgatacgtac tcacccgccc ccgtgttcga tgtgccggcc agcgccataa attcatcaag
    13321 cgtcatgctg cgctgctctt tttttcgtct gaaaggccac atatcacacc cccgctaaat
    13381 ccgcccacca gcggcgatta tccgcacgcg gcattttttc ggggtgctgc tcatacaggg
    13441 aacggcgggc cagctccacg ccggaatcgg ggtaagccgg taccgatgta acggtaattt
    13501 cgtacagttc cgccaccagc acggtgcgca cgcatggatc tgttgtggta tcccatacat
    13561 ccttacggga gcgaaagcca aagctcatcc ccgagatgtc cccacgctta accagttcga
    13621 taacgtcgcg ccctgtactg gtatccggtg gggtcagttc aaagcgtaac cctgtttcgt
    13681 cctcttccag tttcagcgtg ccggaacggg tgcgccccag taacatgctg tggtcatgct
    13741 catacaggcc gcgaacgtca ttacccgccg caagccattc agtaaacgcc ccccgctgga
    13801 atttttcgta aaactcaccc cataacagtt ctgaaagctt atcccagcga acaacgtagc
    13861 ccgtcagcgt accggctccg ctggtggtga tttccgatga ccggatttcc atactcttca
    13921 taattttttc acccataaaa cactaaaggg gctttttagc cccttctgta tgctgttaat
    13981 cgtcgtcttc cggcagtgcc agaatcttga tcgcgttcga atccaccacg ccaccgccta
    14041 aatatttttg cgtgaaaatt ttgatgaatc ctggttctgt caggttgtcc ggtctggtgc
    14101 gaacacctgt ttcgtgatca acaatgtagt aaccgcgttt gaagtcaccc agggcaataa
    14161 cgttatcagg cataaactcc agatattcga ccggaaggcc cagcaacgta tcaggatcac
    14221 ccgcctgtaa acggtcgcgc cagatataat caccgttcgc gttcttcacc ttctgaagtt
    14281 ttgccgccgt cgtggagtta accactcaga ccgcgttttt gcggtatttt ttacgtaatg
    14341 caaatttcag gtcgatcagc gggtccgcag atgtccacgc cagagattcg gaaggtttaa
    14401 ttacctgcaa cgtaccaaaa tcacgctctt tgtcgttctt ctctgcacgg ggtacggata
    14461 aaaaaccttt tgcttttttg tcaccgtcgc ccacaaccag atcgctttct tcggtttccg
    14521 tgaaggtgtc gccaatctca cccgtcagcc atgaaaggat gtccacatcg gaaaaatcca
    14581 cgatttcctg tgtggtgcgc gggtacgcat agaccggata cagcttaatg ctcacctcgt
    14641 taatctgcgg ggtgctggtc tgttcgcgtg ccttaccctc ttcaccgtgg ttaacggtcg
    14701 caccgccagc ggaaacaagc tgcttaaact cgttgctgct gattttcttc acggtacaga
    14761 tgcgacgcat ggtggattca tccgccagca ttcgcatgat ttcggtgttc agttcaggga
    14821 taacggtata accaccatca gcgggaacgc ctgtacttaa tgcgcgggtt tcaccggtca
    14881 gaatgtagtt acgtagttct gcggggtcag tggtctggct gcttttacct ggcttgctgc
    14941 gctcttcgtc tgcaatggct tcaaggcggg aaatgtcttt atcgagggat tcagctttag
    15001 cgcgtaattc gtcaaatttt gcgccctcag catcgttaag actgcggttt tctttttcag
    15061 cgttctccag catgtcgcgc atctgatttt taatggcggt tttctgctgg cgtaattcga
    15121 ttattctctg cataaaaaaa gtcctggggt taagtaagga actccaggac gcggcaaaca
    15181 ctcaaccgtt tttcataagg aaatcagtaa tcgcaccgat cgttttcccg cctggtaatg
    15241 aatattggcg agcacattaa caggcgggaa agtggcccca gcgtcctggc accacgggcg
    15301 agaataatca tgattcagtt cgggtaaaat atgccgatcc gctcagtgaa caacgtggaa
    15361 caaccacgaa caaataattt acaaaaaata acaaaaagcc ggattgctcc ggctgcgtgg
    15421 tgtggttctg atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat
    15481 ttactgcatt tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc
    15541 tctgttttac cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat
    15601 tgccacctca gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag
    15661 tgagcattac gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag
    15721 tggatatcac aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa
    15781 gctcccatga tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag
    15841 ctgcgcttat ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt
    15901 ttggttcgct catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc
    15961 ttcggcaatc tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc
    16021 gcttatcagt tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct
    16081 gattgccacc tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct
    16141 gtctctgcgt tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac
    16201 cccctcgttt aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg
    16261 ccctcatgcc agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac
    16321 cccgtgtcta cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt
    16381 agacagtaaa aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt
    16441 actcaggtag acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc
    16501 tgttgttatt gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc
    16561 gtaaactttc gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct
    16621 cctgcctgcg tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta
    16681 cttcactgaa tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag
    16741 gaactggcaa aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc
    16801 gtcacgctgt tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa
    16861 tcggcggata atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc
    16921 ggcctcacta acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc
    16981 ccgttcggtg aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc
    17041 cgtgaaacgc ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt
    17101 gatgcccgtt ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc
    17161 gacaacctgc gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt
    17221 ctgtttccct gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc
    17281 tccggtggcc tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc
    17341 ggcacatata cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg
    17401 aaagtttagc gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc
    17461 gttgtgcgtg gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc
    17521 aagcgcacca ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat
    17581 aacttttaac gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc
    17641 tgaatagatc accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc
    17701 gccgcgccat acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc
    17761 ggcaagcagc tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc
    17821 cggcatggta agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt
    17881 ttcacacttc tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc
    17941 cgccagcttc tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag
    18001 ccttacgcgt ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt
    18061 tggcccaagc cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca
    18121 ttgtttcgcg tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg
    18181 ttttattccg cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat
    18241 acgttaagca tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt
    18301 ttactgcggg gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc
    18361 acgcaatcat attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta
    18421 atcgttacca tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga
    18481 gtgttgccac tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt
    18541 gttgttcttt atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct
    18601 caagaagcgt tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc
    18661 caagtgtttc aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa
    18721 gaaatgcgcg gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt
    18781 tttcttaaaa taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca
    18841 agcccgttga tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg
    18901 gcattgacga aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg
    18961 aattaaaacg atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt
    19021 ttagcgtcat tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc
    19081 cggtctttct gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg
    19141 tgcatcagcg tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt
    19201 ttccccctga cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg
    19261 cgcttcgcgt tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc
    19321 cagaaaacgc cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc
    19381 aaccataacg gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg
    19441 cggggctttg ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc
    19501 tgaggtgtaa ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag
    19561 ttccgtttag atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat
    19621 gattctcttg ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata
    19681 gaacccaaag gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta
    19741 caggcgcaca caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga
    19801 cgacgcagaa agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag
    19861 aagtacctta aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa
    19921 ccagactgat tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg
    19981 ggatcacaca cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg
    20041 gccgcttttt ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga
    20101 acgaatcccc gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca
    20161 gatgattgat gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta
    20221 tccttccagg ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga
    20281 cgtgatggga attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt
    20341 agcttttcgc attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt
    20401 cgtttcttaa agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga
    20461 tgattcagag ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat
    20521 aaaatccttt aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct
    20581 cgctatttaa agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct
    20641 acttataaat ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat
    20701 tgttgcacgt tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa
    20761 attattttct tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc
    20821 ctaaccgctc cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat
    20881 tataaactcg ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat
    20941 ccacgcctaa atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt
    21001 gagggtgatt tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt
    21061 tcacttcccc cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg
    21121 gcagtattgc ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg
    21181 gcacggtcca gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta
    21241 ccgtccggca tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata
    21301 tttttttgtc cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc
    21361 ttatatccgg ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg
    21421 catagcgccg cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg
    21481 cagtaacagg cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc
    21541 atttatccag cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca
    21601 gggcggcata atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat
    21661 cgccaaccgt taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat
    21721 ttttcccctc tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc
    21781 tcagatcagg ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg
    21841 ccctgtaagt gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc
    21901 caccgtcatt aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg
    21961 tatcgctcag tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact
    22021 acaccattaa ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa
    22081 ggaaaatcac aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa
    22141 cggcaaaaaa cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg
    22201 ttataatcgg ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt
    22261 aactggcccg attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc
    22321 tttaataatt ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc
    22381 gaagttgaca gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg
    22441 ttcgaaagct acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac
    22501 cactggcatg agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt
    22561 attgatgatc tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat
    22621 cagtacgacg tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag
    22681 ctgcaaatga aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc
    22741 atcatcgatg aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat
    22801 gaaggctacg cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg
    22861 cgggaattaa ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac
    22921 tacagcgatc cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg
    22981 gataaactgc gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg
    23041 tttggcgagt ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct
    23101 tatcagccag atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt
    23161 ggtctgcgcg tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc
    23221 ccgcaccgtc cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt
    23281 ggcgatgcgc tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg
    23341 tattggttct tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc
    23401 ccttttcgct tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc
    23461 tttttccata ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt
    23521 gaacaggttc gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg
    23581 gatcggtttc agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag
    23641 aatatgcgga tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa
    23701 ttcaatgtag caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt
    23761 acgtttacgc gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc
    23821 ccgttcaacg gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat
    23881 ttccgggctt tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt
    23941 taccatcact cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac
    24001 cgatgcggcg ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta
    24061 agcccagcgg gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc
    24121 atccggggta aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc
    24181 cagcgcggtc atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc
    24241 atcggtggta atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga
    24301 taaaacttca tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg
    24361 cggattaaca tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat
    24421 cgccgggata tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact
    24481 taaggtcaca ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt
    24541 ccattggcct tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga
    24601 cacgtagttc aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac
    24661 aacattatta agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag
    24721 cgggaatatt tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa
    24781 agattggcga ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt
    24841 ggttaaccac aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa
    24901 aaattgaata aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa
    24961 tgctttgatg gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc
    25021 ctcacgctgc ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt
    25081 ctggaaactg ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg
    25141 gcggaaaacc gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc
    25201 gtgttcgaaa atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg
    25261 ccccgcgtga tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg
    25321 catcagctct ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag
    25381 cctcgtctgt tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa
    25441 atgcagaacg agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact
    25501 cacgaccagg aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc
    25561 attgagcaag acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc
    25621 gggttcattg gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag
    25681 cgcgtacgcg ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa
    25741 ccggggcaaa aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac
    25801 gacgacaacc acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg
    25861 acgctggagt cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc
    25921 aatgaagacg atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta
    25981 gaaagctggg aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat
    26041 tgtcactatt gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg
    26101 caccagcttt ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa
    26161 ctacacgcgt ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc
    26221 gctgatcgcc accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa
    26281 gctgccacac aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa
    26341 ctcattgatt cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga
    26401 gtttttgctc tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc
    26461 ggtgattatc ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag
    26521 tatcgaaaaa ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt
    26581 acagactttt atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct
    26641 gctggtgatg ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa
    26701 aaacctgctg atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt
    26761 tggtgcagcc accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg
    26821 gcgcgcttct cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc
    26881 ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg
    26941 aactccttta acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat
    27001 agcctgctga tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg
    27061 gtgttttcgg cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt
    27121 tatcgctttc gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca
    27181 gatatcgtga tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc
    27241 ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg
    27301 tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc
    27361 agcgaattta ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg
    27421 ggctgggtgt taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc
    27481 ggaccaagtt atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg
    27541 ccggaagtta acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc
    27601 agccagctta ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat
    27661 ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg
    27721 atgacaacaa cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg
    27781 aacagttcac caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa
    27841 ccatgtacgc caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa
    27901 cctattacgt cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt
    27961 taacgaactt cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg
    28021 actactccat tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg
    28081 atccgaaatc tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc
    28141 tgttgaccga cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg
    28201 gtaacaccac cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc
    28261 caaacgtcgc agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta
    28321 acctcggcat gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg
    28381 acgtggtgtg gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa
    28441 atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg
    28501 caaaacaggt tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt
    28561 taagtccaga agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg
    28621 gcgaatggca gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc
    28681 tgaaagcagg acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag
    28741 caaggatgtg gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg
    28801 tggtccgctt gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc
    28861 cctccagcaa cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct
    28921 cggcaaattc attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat
    28981 gtagtttggc ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg
    29041 caataaagac atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt
    29101 cgccaaacca aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat
    29161 cttctggcgt aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt
    29221 tcagcaattc cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt
    29281 ctatattctg cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg
    29341 caagatgcgc ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat
    29401 aaaacgcttg caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa
    29461 cccgatgttc ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag
    29521 aaattcctgc ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt
    29581 tatctctgaa aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa
    29641 aacgacttaa ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta
    29701 gatgtaggaa ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc
    29761 gtggaacacg agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga
    29821 ttgccgggaa attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca
    29881 ggatatttcc cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct
    29941 gctcatcgcc ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat
    30001 agtgttgata cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct
    30061 gaccacagcc gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg
    30121 gcagacgcat atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt
    30181 tgaaaaccag cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt
    30241 gcgtaaattc atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc
    30301 gtacatcgcg atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg
    30361 ggacattagc ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc
    30421 cgacagaacc tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat
    30481 ctaaaaatgc gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc
    30541 gaccgctatc aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca
    30601 ttgccgctcc ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca
    30661 atattactgg cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg
    30721 accagtacgt agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa
    30781 atatcgctgg agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta
    30841 agttgcagtg aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc
    30901 agcaatccat accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt
    30961 aatactgcga tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg
    31021 aaacaggcca cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg
    31081 atatcgcgat acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg
    31141 gtcacttcac ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg
    31201 gcaatacctg acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc
    31261 atggcaaaac tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc
    31321 acacgttttg gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc
    31381 caatcgccct gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt
    31441 tcgcccgctt taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga
    31501 cgctgttcct gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt
    31561 tccaccagac cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc
    31621 acgttatcca gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca
    31681 tgcggcacca ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt
    31741 aagccgacga tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt
    31801 agcgacacca tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat
    31861 aaaggcatcg ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac
    31921 gcatttccag ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga
    31981 aagcggtgcc ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac
    32041 gcgcatcgag gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg
    32101 cacgggcaat cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg
    32161 cacgatgctc cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg
    32221 gctttttctt gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca
    32281 gcaggtggtg aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg
    32341 ctgaagacag tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat
    32401 ccagcccgcc taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg
    32461 ccatcatttc accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac
    32521 tgccttcctg atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt
    32581 aaagcctcag cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc
    32641 gcgatagcca tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc
    32701 agcgccgcgc catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca
    32761 agcagtgcgc caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc
    32821 accataaaga ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact
    32881 tcgccctgct tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca
    32941 gcgacaatca ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac
    33001 agctcgcctt tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga
    33061 cttaaagagt caactctcag cggctcatcc agccacaaac gccagccggt aatattggct
    33121 gccggataac gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg
    33181 acttcactgt tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc
    33241 cccatcggcg tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg
    33301 acgcccaact gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg
    33361 tcagtttgtt tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata
    33421 ccgagcatca ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta
    33481 ataggtgcga cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg
    33541 ttaagagagc catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt
    33601 tgcagctcgc gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg
    33661 agggtaatgc cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc
    33721 ccacgcatgt aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg
    33781 ttgctgtagc aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt
    33841 aagtgatcgt cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat
    33901 tttatcatca atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac
    33961 ccattacacc tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg
    34021 ttgttctgat gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc
    34081 tggtggtgat ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc
    34141 cgttatcgga agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc
    34201 gtatgccggt ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac
    34261 cgtggggaaa ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg
    34321 gcgtggtgca gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt
    34381 taagcaatgc ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga
    34441 tcaccgccag caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta
    34501 aaatttttag ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg
    34561 ttaatttcgt tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt
    34621 acagccttgg cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc
    34681 gccgccatct gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta
    34741 ctatctcgct gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt
    34801 acgagcaacg attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca
    34861 ttgctatcta caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc
    34921 aaatgaacgc agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct
    34981 ttgtcagttt gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg
    35041 cattcagcga tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt
    35101 aaaccatcac cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga
    35161 acagcattgc ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg
    35221 acaaccgtta tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt
    35281 ctgctgggcg agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt
    35341 cacgccggtc cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat
    35401 gaaatcagcc agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc
    35461 tacgacagtt tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag
    35521 ctaccgacga tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt
    35581 tgcccacaca gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca
    35641 cgagatgcat tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg
    35701 gagcacggcg aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag
    35761 ctgccttatc gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc
    35821 gacagccagc gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt
    35881 ccgaccgata aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg
    35941 aagcgcgatc cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc
    36001 gagtactggc agccattgtt cttcagcgaa ccactgccac cgctgttcag ttatttccct
    36061 gccaatacct tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct
    36121 gacacgctgg cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca
    36181 caatcgctct ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg
    36241 cagctaaaaa ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa
    36301 ctgccagacc tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc
    36361 ctcgagactt tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg
    36421 ctgggtgaac tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa
    36481 gccagcgacc gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag
    36541 gcgcgtaatc tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt
    36601 cgtcaggatt ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg
    36661 catattggtc agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc
    36721 acgctggaag ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc
    36781 aaactgtatg ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa
    36841 gaaaacgccc cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg
    36901 gcggaaaaag tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc
    36961 aaagagggct tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc
    37021 ccgtttgaaa ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt
    37081 cagccgctgg caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg
    37141 gcgatgcgcg ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct
    37201 accacccttc ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg
    37261 gtacgtatcg aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg
    37321 gaagtggcgg aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac
    37381 gtcaagttta aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt
    37441 cataaagagc gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg
    37501 ccgatcccac gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc
    37561 acgccgcccg cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg
    37621 gtccgggagg cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat
    37681 gatgtggaaa acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg
    37741 attgccatcg gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc
    37801 catcatcaac gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc
    37861 ccgacagcca acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac
    37921 cagttacgcg gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg
    37981 catccaaaag cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa
    38041 gatctcgggg caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa
    38101 ctgcttggcg aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag
    38161 ttgctggaaa acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc
    38221 accagccagc aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc
    38281 cctgacgtga acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac
    38341 gaactggaag agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg
    38401 cgtaccctgc tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag
    38461 ctggaaggta atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg
    38521 gcctggttga ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg
    38581 cgcctgaaat ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag
    38641 tttatgcgtg aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc
    38701 ccccatctgc aacatttaca aattctttgc actttcctgc actatccgac actgtcacca
    38761 tccataattc aggcttatct gtttattaca ataaccttat atttattatg tatttttggt
    38821 gatgatcaaa acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc
    38881 gctggcgcta ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt
    38941 tggcgaaaac aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa
    39001 aaaatataac gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt
    39061 accgcgcgcg ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg
    39121 cgaaggcatt gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa
    39181 ttcggtaacc gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac
    39241 aatggtgacc accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat
    39301 ccgcgcccgt tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga
    39361 taacccaatg ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca
    39421 cggtacgaac gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg
    39481 ggatgacgat atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat
    39541 caacactccg ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca
    39601 gccgctgtca gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc
    39661 aatgcaatca tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga
    39721 tgtccgttcc gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta
    39781 aatcctggat aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag
    39841 caggcattaa tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc
    39901 ggaccggtta cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc
    39961 agctgctctt ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt
    40021 tgttggcctt ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag
    40081 cttaaaatcg ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat
    40141 gaatgacgtc gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat
    40201 cacatacaag atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt
    40261 caaatagaaa gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta
    40321 taataacctt tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca
    40381 atgtgtaaaa aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa
    40441 ggccatgaaa ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga
    40501 agcaaccgga gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt
    40561 tcgtgccgtt ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt
    40621 ttgtgaagag aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg
    40681 ctttaccagc aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc
    40741 cccagattca ggagatatag ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac
    40801 tttgcagcag tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt
    40861 gacacatctg gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga
    40921 aggtgcatcg ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga
    40981 actggtgaaa taagtcgcaa aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc
    41041 agagtacagc cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg
    41101 atctgcgtac ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag
    41161 gtcacatcat aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc
    41221 gtagtcgtgt aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca
    41281 ccacccagcg cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc
    41341 cctatcacgc caccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc
    41401 accggtcgac gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt
    41461 tccttgattg gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc
    41521 agactggcca ctgccgctac gcccaacgca gctgcgacac caatcccgat acccgccaac
    41581 attgatttat tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg
    41641 agaaaagcag ctatcagaca gaagatgaaa agcgcgggaa tgactggaaa tacttaagga
    41701 ttcggaggag tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg
    41761 ctcgtaccat ttgtccaggg cggataaggc gtttatacca catccgccag tgtacgtcga
    41821 ttaatgcaac ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa
    41881 tccggtttta aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac
    41941 aaagcgcgca attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct
    42001 accaacggtg gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt
    42061 tttcagcggc ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg
    42121 gtgtgcagcc tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca
    42181 gtcgccaata gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag
    42241 ctggttgata cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat
    42301 cccggctgcc cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc
    42361 gccttcatca gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag
    42421 ctcgttgtgg gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc
    42481 ttctaccagc gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag
    42541 ttgcttgatc gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac
    42601 aatcgcaatg ttcactttgc catttgcgcc caggttggca gagtatttca ggaacagatt
    42661 cagcatctcc tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc
    42721 tttgacgcct ggcgtattga aatcgttaga ggtgctaccc agcgccatga ccagggtgtc
    42781 ataggcgatt ttacgttccg gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat
    42841 agtgattgtt ttcgcttcac gatcaatatc aatgacggaa cccaactgga actggaaacc
    42901 atgattgcgc gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt
    42961 cgccacttcg tgcagcagtg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat
    43021 tttggctttt ttcttacgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc
    43081 agcaccgccg ccgacaatca caatcttttt caatggcgta gtcaacgtgc cccccttaaa
    43141 tttattaacc aattgttaat taatggttat taacatagct cttaattaac aacaggttac
    43201 agcaatgaaa atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa
    43261 attgatgtta atcaagtttt gttgcttaaa agataggcaa ctttaccggg ggagatcaaa
    43321 aaatagagtt tattgtttat tatttgagca aattcggaag ggtaatatag ggtgaagcgt
    43381 atatcgaggg cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct
    43441 cgactgtcgg atgcggcgtg gacgccttat ccgacttaca gaattcggag cgcatcaggt
    43501 accggcaagg ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg
    43561 cgctgtaaat gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc
    43621 tcgtaataat gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc
    43681 gacaaaataa ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg
    43741 gcaatatcgg catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac
    43801 aaattagggt tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag
    43861 tatccgccca ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt
    43921 ttgtccactt ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt
    43981 atcaagcgta catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga
    44041 ctgttagagt caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta
    44101 gtgacctact ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc
    44161 tttccagcga gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg
    44221 tgtaacacgt tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg
    44281 attattgcag accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc
    44341 ataactgccc ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc
    44401 aaaccccagt tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg
    44461 cggatcaaca tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg
    44521 gattaaggaa ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc
    44581 gcacggtgtt tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc
    44641 ggtcgttttc attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc
    44701 tttttgtgga tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc
    44761 cagcactggc gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca
    44821 acccgcctct tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg
    44881 tgctgccggt aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc
    44941 taccgccacc accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa
    45001 ctgggcagga tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc
    45061 cagtatttca cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc
    45121 cacactgctc tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga
    45181 taaattgttg atcgccggtt tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca
    45241 gcagccaggg aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc
    45301 gagtggcata gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg
    45361 ccagttccag cgcgatatca ccatctccag catactccca gtcgatgagt tttaaccctg
    45421 atgcgctatg cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag
    45481 gtcgtggttc ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc
    45541 gcgccggatc gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc
    45601 caaaacgtgg ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca
    45661 aatacgtttt taccgcaccg ggcagatagt cgactaccat ccagtcacgg agatataaat
    45721 gcggcttcgg tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg
    45781 cggactgcgg cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt
    45841 caatgagaaa actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg
    45901 ggaaaaagcg cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct
    45961 ttacctgacc agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga
    46021 gcgttaacgt tgccagaggc gcaggagtac agcacgtaat gagcgccgac attgcgggca
    46081 atgcctatgg ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc
    46141 ttagccatcg acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca
    46201 tttcgcagcg tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg
    46261 ctatcaacca gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc
    46321 atcggctgca ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt
    46381 cgatcttcgt gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg
    46441 gggacaggct gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt
    46501 gcaggttcac gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg
    46561 tagcgactca tttttgtcat caagattcac ccctttacaa atatagataa agtctgactt
    46621 tgtgcgcccc cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg
    46681 taacgctgcg tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa
    46741 aacgataatg aacggtaacg ggttcttgcc tttcgttata aatcgttgag gatgctgaag
    46801 gttgaatatc agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag
    46861 atgactccat caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc
    46921 ccaccagcag taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag
    46981 acctttatgc gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg
    47041 gtaaacctct tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc
    47101 aataccttct tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc
    47161 atgctcagct gacacgtcgt tcacagtcgg aatgagaata ttcggaatga tcagaatatg
    47221 cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat
    47281 atcggagggg atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt
    47341 ttcctttttc aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag
    47401 agtatggtcg agttaaacct gctctttcaa tcttaactca cgatttctta agcaaaaaat
    47461 cgttttgctg ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag
    47521 acattctctg tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttgc
    47581 agcaaaatta ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga
    47641 cgacaacata aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
    47701 atcaagtcat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
    47761 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
    47821 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
    47881 agatgactca acatgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag
    47941 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
    48001 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
    48061 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata
    48121 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
    48181 gtaatccatc tgcggcaatt aatatggttc gaaaacacgc gacctgtcgt gaatttaaag
    48241 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
    48301 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
    48361 actcatggct ggaccgctac aatagtgaaa agaccttctt ctcgggcatt gtcgatgctg
    48421 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
    48481 gcccaacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
    48541 gcgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
    48601 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
    48661 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
    48721 tgctggttgg gccatacagt aattatggac ctggctttga tcatgtcggc ggcaccggtt
    48781 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
    48841 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
    48901 cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
    48961 tttcccacaa gccgactggt catcacagag cctggcgcag gacgatacca cacatatgaa
    49021 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
    49081 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
    49141 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
    49201 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
    49261 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
    49321 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
    49381 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga
    49441 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta
    49501 tcttgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
    49561 aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa
    49621 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
    49681 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
    49741 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat
    49801 cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
    49861 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
    49921 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatagcctg
    49981 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
    50041 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
    50101 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
    50161 tgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
    50221 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcggtaacag
    50281 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
    50341 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
    50401 gtggatgatg tacaggatcg gcgcaacgaa catgaagaag aactcgatcg gctcggtgat
    50461 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt
    50521 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    50581 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    50641 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    50701 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    50761 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    50821 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
    50881 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    50941 gaaacggtta aacatgtacg ctgcaatcgc accggagata atccctccga gtacgccagt
    51001 atccgccaag tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    51061 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    51121 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    51181 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    51241 cgcggaaccg acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag
    51301 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    51361 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
    51421 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
    51481 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    51541 atatgtcaca atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac
    51601 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    51661 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    51721 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    51781 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
    51841 tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac
    51901 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    51961 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    52021 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    52081 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
    52141 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
    52201 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    52261 gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac
    52321 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    52381 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    52441 gtctctctta aagatgagga accggtaaca caacgcccgg ttgcaggtaa tgctcaatac
    52501 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt
    52561 cacgaatgtg gcaaacatcc cccagtatag cctgcaggca cgagggagaa agatggtttg
    52621 ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgcgcagcg ccatgatggc
    52681 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    52741 gattaagggc cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct
    52801 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
    52861 gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    52921 gtgaaagcca agtcacggcg tactgttctg gaggcggcgc aaggtaatgt aaccgacaac
    52981 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
    53041 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    53101 cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt
    53161 cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg
    53221 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    53281 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    53341 catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat
    53401 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    53461 ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    53521 ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg
    53581 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    53641 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt ctgggcgaaa
    53701 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc
    53761 gccctgatac gcctgagtgg aaagatcgtg gcgatcgcca atcacccagg tgccgttagc
    53821 cagcgcaggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    53881 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    53941 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    54001 cagttgctcg agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    54061 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    54121 gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    54181 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    54241 ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    54301 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    54361 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    54421 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    54481 gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    54541 ctttcaccat tttcttgtga actcgcttga gtaacgcgac atcggtggta ttggcggtat
    54601 acatcaaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    54661 cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac
    54721 gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg
    54781 cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    54841 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa
    54901 cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg
    54961 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    55021 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    55081 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    55141 atataacgtt gctgacgaaa aggtgacatc gccacaggca cgtacgggca tcactggcat
    55201 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcctccagcg aggcttgcac
    55261 ttcgacaagc tgataagagg attgtgccag taaacggata cagaattgtc gcataatgcc
    55321 gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa
    55381 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    55441 gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agccgattaa gatgtttaat
    55501 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc
    55561 gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgctgg
    55621 tccgctgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    55681 cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa
    55741 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgcaggatat gcgccgataa
    55801 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    55861 tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc
    55921 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    55981 gctaagaaaa aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc
    56041 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    56101 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    56161 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    56221 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    56281 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    56341 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg
    56401 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    56461 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    56521 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    56581 agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    56641 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    56701 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg
    56761 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    56821 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    56881 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    56941 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    57001 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    57061 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    57121 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    57181 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    57241 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaagg agggaaaaat
    57301 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    57361 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    57421 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    57481 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    57541 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    57601 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    57661 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    57721 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    57781 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    57841 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    57901 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    57961 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    58021 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    58081 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    58141 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    58201 aaacagatga aaggttagtt tcgataatat cgttccactc ttcatctttc attcgcatta
    58261 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    58321 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    58381 ctttgccgtt ggcacctaaa taatcactga tcgcctgagc gccattttca ctggtcgcag
    58441 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    58501 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    58561 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    58621 caatgcgttt cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta
    58681 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    58741 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    58801 caacagaaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    58861 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt
    58921 ttgcgccagc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    58981 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    59041 cttcacacgc tttcgcaata ggcgcatcat ccagaccgat gattgccgcc atagcgcccg
    59101 tgccttccgg tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat
    59161 cagcgaaatc aatcacgcca gcgcaaacca gcgcggagta ttcccccagg ctgtgaccgg
    59221 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg
    59281 cagtcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct gacccctgct
    59341 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    59401 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    59461 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcag
    59521 agccccaagt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    59581 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    59641 ggcgatccag cgtcaccacg acattgtcca tcgacatacc gagttttttc gccgttgcac
    59701 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    59761 tattcgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    59821 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacgcgg tcggcgtttg
    59881 gcagcgtcag caattcacca taactaccgt cggcatgcag atgggtggaa atgattcccg
    59941 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    60001 cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    60061 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcac
    60121 aggctgctgc aacgtcaaat gccgggcaac ctttaatgtc caacatgctt tgaatctgac
    60181 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    60241 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    60301 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    60361 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    60421 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    60481 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    60541 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    60601 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    60661 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    60721 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    60781 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    60841 gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg
    60901 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    60961 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcaagaccc
    61021 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    61081 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    61141 acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc
    61201 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    61261 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    61321 agggccatac gcattgaact cccacggctg gcgcggatag cttgcgaagg ccgggcatca
    61381 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    61441 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    61501 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    61561 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    61621 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    61681 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    61741 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    61801 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    61861 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    61921 atacggcaaa tgggtttggc ttttgcactt cttcaggcag ttcaccaaag accatgtccg
    61981 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    62041 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    62101 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    62161 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    62221 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    62281 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    62341 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    62401 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    62461 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    62521 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    62581 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    62641 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    62701 acctgaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    62761 ggcacaagaa aaatcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    62821 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    62881 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    62941 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    63001 tcacctaagc gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc
    63061 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga
    63121 ccctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    63181 gaaaaacccg ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat
    63241 cggtgcaggc ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    63301 ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat
    63361 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    63421 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    63481 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    63541 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    63601 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    63661 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    63721 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    63781 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    63841 ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    63901 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    63961 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    64021 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    64081 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac
    64141 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    64201 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    64261 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    64321 tatgcttatt tttagttttg caccttatta cttcactacg tgatcacttt attgatggtt
    64381 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    64441 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    64501 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    64561 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    64621 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    64681 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    64741 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    64801 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    64861 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    64921 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    64981 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    65041 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    65101 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    65161 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    65221 tatctggttc tgatgccaaa taacccgcgc gcaggtggca tttctcgccg tatcgaaggc
    65281 gatgaccgta ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg
    65341 cttatcgtgc gcaccgctgg cgtaggcaaa tctgctgagg cgctgcaatg ggatttaagc
    65401 ttccgcctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    65461 ctgattcatc aggagagcaa cgtaatcgtt cgcgccttcc gcgattattt acgtcaggat
    65521 atcggcgaaa tccttatcga taacccgaaa gtcctcgaac tggcacgtca gcatatcgct
    65581 gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    65641 ttcagccact accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg
    65701 ccatccggtg gttccattgt tatcgacagc accgaagcgt taacagccat cgacatcaac
    65761 tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa
    65821 gccgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    65881 gacttcatcg acatgacgcc agtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    65941 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    66001 gaaatgtccc gccagcgcct gagcccatca ctgggtgaat ccagccatca cgtctgcccg
    66061 cgctgctccg gtaccggtac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    66121 ttgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    66181 ccaatcgctt cttatctgct gaatgaaaaa cgttctgcgg tgaatgcgat tgaaacgcgt
    66241 caggacggtg ttcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac
    66301 gtgttgcgcg tgcgcaaagg ggaagaaacg ccgaccttaa gctacatgct gccgaagctg
    66361 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtcctgaa
    66421 caacctgcac tggcaacctt tgccatgccg gatgtaccgc cagcgccaac cccagctgaa
    66481 cctgccgcga ctgtcgtagc cccagcaccg aaagctgcaa cggcaacacc agcagctcct
    66541 gcacaacctg ggctgttaag ccgcttcttc ggcgcactga aagcgctgtt cagcggtggt
    66601 gaagaagcca aaccgaccga gcaaccaaca ccgaaagcag aagcgaaacc ggaacgtcaa
    66661 caggatcgtc gcaaacctcg tcagagcaac cgccgtgacc gtaatgagcg ccgcgacacc
    66721 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    66781 gcacagcagc aaactgccga gacgcgtgag agccgtcagc aggctgaggt aacggaaaaa
    66841 gcacgtacca ccgacgagca gcaagtgccg cgtcgtgaac gtagccgccg ccgtaacgat
    66901 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    66961 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    67021 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    67081 gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg
    67141 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    67201 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    67261 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    67321 atgccgttga ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc
    67381 tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta
    67441 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc
    67501 gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg
    67561 caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag
    67621 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa
    67681 ccgatggttg aaccgcagga agagacggca gatgcagata ttgaagaagt tgccgaaact
    67741 gctgaggttg tggttgctga acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct
    67801 gaagtcgcag cagaagttga aacggtggcc gcggttgaac cagagatcac cgttgagcat
    67861 aaccacgcta ccgcgccaat gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg
    67921 cgtcacagtg actggcagcg ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt
    67981 catacggcaa cgcatcatgc ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa
    68041 ttagctcaaa gtaatcaagc cctggtaact gccagggctt ttttatttca tgtttgaatc
    68101 aacatattaa aattcatcaa atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa
    68161 aaatgcccgc cccggcgatc ccagagcggg cagacaaaac atacccagtt tcatgatatg
    68221 ttttaaagcg agcttatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg
    68281 ctttatacga tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc
    68341 agtccacatc aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta
    68401 aggaatccag cgactccagt ttgttcagct gcgtgcctaa ttccgcgcga acggtaagga
    68461 cattattcag cgagtttttc agtccacggt tggttttatc caatgccgca gcggcgattt
    68521 ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg ctatccagca
    68581 tggcaaaaag attggtttca gaagcgctac cgtctggttc cgctaccgcg ttactggtaa
    68641 tactgtcgaa aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct
    68701 gttgcttaat actttctgtt ccgccaacat aatcaccgtt agcctcacta aacggcgcag
    68761 tctctgtttt atatgtagcg cccattaccg tcagtggtgt tcgccagatt cagcaactgg
    68821 tcacgaagcc cctgaatatc cgtagccagc gaggcccggt catcgtcact caaggtgcca
    68881 ttgctggcgt agacaatttt ttcctgagca ttctggatag cagtggtgac ctggctaagt
    68941 acactctctt ccagtgacac tttttgagtg gcgaaagtac gtgccagcgt gtactggctg
    69001 ttttgcgcct gtgcctggga gagaacaaca gcttgtgatg cagcaatggg atcgtctgaa
    69061 gggttaacga ctcgcttacc cgtcgacatc tgctcgccgt acttcatcca ttctgcctga
    69121 gaattggtga tgccgcgcat gttttgctgg tacatcatct gggtactgaa acgcatttta
    69181 tctcccctta gcgaatgtta atcagcgcat caaaaatcgc gtttgccgtc tgcagaacct
    69241 gcgcattcgc caggtaatac tgctgaaaac gttgcagatt tccgtactcc tcatcgagat
    69301 tgacaccgga aatcgactgc tgctgattgg aaagctgcgt caccacatta ccttgcgtgg
    69361 tgctactggt tttcaacgtc gcggttttat taccgatatc actcactaac gaggcataag
    69421 cgtcgttaaa ggatttcgca ccgcccaccg ttttactgtt gctttgcaga tccagcaggg
    69481 cctgaccgtt gcggttgtcg ctatcacccg catcttcttc gctcgccatc gcgattttcg
    69541 cttcgtcggt gattaataca tccatgttga cgatggcgtc acttactggt ttcagcgtga
    69601 agctgtcgtt aacggcaggc gttcctgtaa acgtcaactc cagaccatca aatgccactt
    69661 taccgttggc atctggtgtc gccgtaaaag tggtattgct ggcaaggcgg gtgacctgcc
    69721 actgattatt atcgaacgag attttgtaat ctgtcgccag taccacggag gcatcagtta
    69781 ccgtggcacc gatcgcaacg tcacctttgt ttttcgtgtt ttgcagaacc gcgggcttac
    69841 cgatagtaaa gaaatcttca ccagcatcgc cgttagcatc aaatccggct ttgtgttggc
    69901 tgttgaaagc ctcggcaaat gccagcgcca gttgtccaag cgtattacgc gtctggtcca
    69961 gatcctgaga acggaatgtc agaatgccgc ccagcgaccc ggtattcagt aatttctccg
    70021 ggatctcaat attgcctgcc gtcccatcaa cgtaagcgac agtcgtacga gaagggtcgg
    70081 cgctggaagg aactgccgcc agttgccgcg ccgtacttcc ctgaaccagt gagtaaccat
    70141 tggcatcgtg atgttataag taccgccatc ctgaacgctg acttctacgc caacaatctg
    70201 gtttaattcg ctcaccagtt gatcgcgttg atccagcaga ttgttaggtg acgcccctgc
    70261 ccccacgcct gtcaggcgcg agatttgatc gttcaggctg gcaatttgtt tagcgtaatt
    70321 gttgatctga tcaacgctgg cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag
    70381 atattgatcg gtggttttaa actgattcac caacccttct gatttcccaa tcagcgcctg
    70441 gcgcgccgcc gggtcttccg cgttactcac cagcgtttgc aggctggtga agaaatcctg
    70501 catctgtgtt gccagcgaag aggtactggt ggagagcata ttgtcgattt tcgacatctg
    70561 ctcatagcgg gcggtcaggc cgctactttg cgtctgcgca gcacgtaact ggttggtaat
    70621 aaacgtatca tactcacgct gcacaccaga aacgtagacg ccattgccaa cccagccgcc
    70681 agcgcccaac gtgctattgg cctgcgccat aatagtggtt tggcgggtat atccggcaac
    70741 gttatagctg gagatattat tacttgccgt atttaacgcc gcctgggccg cgttcagtcc
    70801 gctcatggcg ttattgatca agctggacat ggaggttcct tataagcctt caattacaga
    70861 gtattatcgg cagcgacccg ccggacttga gttattcaga acagattatc aatgttcatg
    70921 ctgtaggttt tgctcacctt gtcgcttatc gatttcatct gctggatcat gttggtgagt
    70981 ttacgggcat agtgaggatc ggtagcatag cccgcgtcct gtaggacctg cgccccctgt
    71041 tccgcactcg cggcggtcgt cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg
    71101 taatccgaca atgcttccag atacgagctg tagacccgaa acttcgcttt tactttcttc
    71161 gcttcgccgt tttcatattc agtcgtggtg atttcagtga cttgcccttt ccagttgcca
    71221 gaggctttga caccaaacag gttatagctt ggctcgccgt tttcgcggcg gatttgccgt
    71281 tgcccccaac cagattcaag cgcagcctga gcgaggatca aatgatgtgg cacaccgctt
    71341 tgctggcttg ccagttgggc gggcagcgag agttgcgcga ggaatgctct actgtcaccc
    71401 ggcagcgaat catcgtagtt acgtggtacg gccttttgca ccagctgcga aagcgtctgg
    71461 ttttgataac gcaccacggt ttcgagcggg aatttcatcg gtgctgctgg cgtggactcc
    71521 tctggcaatg gttgttctgg cgtcatctgt ttaaccatca tctctgcaag ccccagacct
    71581 ttgcccgccg tcatctgttg ggcaatttgc tggtcataca tactggtata cagacgagtg
    71641 tgctcgctgc tgaacaggcc atcttttggt aaagcgtcgc gcatgctttt caacatcatc
    71701 tgcacgaaca tcccttccac ctgacgggcc accggacgga tatttgccgc cggatcttcg
    71761 ctcgctttcg cctttagttc gttgagtgat tgcgcatccc aggccgcact tgccagtagt
    71821 ttgctgtcgc tgatcatcag atgatttcca gttttgcccg cagacatccc gcactttgca
    71881 ttgattgcaa aatagacatc agatccatcg gcgtagcgcc cagcgcattg agcgcacgca
    71941 ccacgttatt gaggctggcg ctggaacgta cgctttgcag cgaaccgccg ctctggcgta
    72001 aatcgatctg cgtttgtgga gtaacaacag tctgtccacc accaaacggt gtatctggct
    72061 ggctgacatt ggcctgacga ttaactgtta ctgagagatt tccctgcgct accgcgcagc
    72121 tgtcgagggt cacttcgcga ttcatcacca ccgaaccggt gcgcgagtta atcactactt
    72181 tagcgtcctg cggggtgaca ttaacctgca tattctggat atcggcaagg aaacggacct
    72241 gggaactgtt gccacttggt acgcgcacct gaatagtccg cgcatctaac gcggtggcgc
    72301 tgccatatcc acgcacgcgg ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt
    72361 cgtcgttaag ttgcaaatta agggtattcc cgacgccaaa ctggctgggc aattcacgtt
    72421 caataaccgc accattggtg atccgtccac cgttcagttg gttaacctga acactgctac
    72481 cgcctgcgga ggctcctgcg ccgccaacca gaatattgcc ctgcgccagc gcatacacct
    72541 gactgtcaac gcccttaagc ggagtcatca acaacgtacc tccacgcaag cttttggcat
    72601 tacccatgga agaaaccacc acatcgatgg tttgcccctg acgtccaaac ggaggaagtg
    72661 acgctgtcac cattaccgca gcgacgtttt ttagctgcat attggtgccc gtcggaacgg
    72721 taattcccag ctgtgagagc atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg
    72781 tctggtcacc agtgccatcc agccccacta ccaagccata gccaatcagt gagttttgcc
    72841 ttaccccctg aacactggtg agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca
    72901 gtagaagaat taatgcagag agaaatttaa tcaccacagc ctcacttaca ttggcgacag
    72961 gttaaggaag aaacgctgca accagcccat attttgcgct tcgttaatgt agccattgcc
    73021 tacgtattca atacgcgcat ccgccacctg agtagacggt acggtattgc tgccgctgat
    73081 agtgcgtgga ttaaccacgc ccgagaagcg aataaattcg gtaccctgat taatggcaat
    73141 ctgtttttca cccaccacat gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt
    73201 caacgtgccg ctaaaggtat tgctggcatt ggccccgccc tttccgttga acgtgttacc
    73261 accggaggct tcgacatcgg cacgagcgtt accaaacagc ccctgcaaat agcgcggcac
    73321 agtatcaaag ccaaaattag ttttaccgtc acggctggca ttcgcagagg agcttttgct
    73381 ggcgctgacg ttctcctgca acacgatggt cagcgtatcg ccaatattgc gtggtcgacg
    73441 atcttcaaac agcggttgat agccatagtt aatcggctga gcagactgga aaatagaacc
    73501 gttggcgacg ggcgtcggac cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg
    73561 cgtggagggt atccaggcgc agccggttag tgaaagcacc aacaagctgg aaatggcata
    73621 agtatgcgca gcgttttttt gcatggctat catcttcaaa aatcagtaaa ccggtgaacc
    73681 tgccaccggt tatgccttag agttgcgtca gtttttgcag catctgatcg gtggtggaca
    73741 ccgctttact gttgatttcg taagcgcgtt gcacctgaat catattgacc agttcttccg
    73801 ccacgttgac gttagacgtt tcaacatacc cttgatacag cagtcccgcg ccgttcaggc
    73861 ccggcgtgct ttcgttcggt gcaccggagg attgcgtttc ggtgtagagg ttttcgccaa
    73921 tgctctccag tccggtatca ttcataaagg tggtgagatt gagctgccca acctgaaccg
    73981 gagctgcctg gccttgttgg gttacgctga ccacgccatc acgaccgatg gtgatactta
    74041 acgcattcgc cggaatggtg atcgctggct gcacctgaaa accaccagcc gtcaccagct
    74101 gcccgttctg atccacctgg aaagagccgt cacggatata ggctgatgag ccatctggca
    74161 acattacctg gaaaaagccc tgccctttaa tcgcgacatc tttgctgtta ttggtctgcg
    74221 acagattgcc ctggctgtgt aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt
    74281 gtaatccgga gggtaaggtg gtttgttcgg aagactgtgc ccccggctgg cgaatggttt
    74341 gataaagcag atcttcaaac accgcgcgct gacgcttaaa accgttagta ctgacgtttg
    74401 ccaggttgtt ggcaatgacg tccatattgg tttgctgggc gtcaaggccc gttttggcga
    74461 tccataatga actgatcata taatgtcctg tagtggattg ctgacaagca gagcagaagc
    74521 acgcgacagt gtgaatttcc cctcacccta accctctccc caaaggggcg agggaatagt
    74581 ccgcgcgctt ttattgcaat gtcatcaatc gttcaattaa ctcatcgaca gcagttggtt
    74641 ggcacggcct gcgttatcat cgacgctgct gatcaccttc atctgcattt caaaacgccg
    74701 cgcgctggca atcatgtcgc tcattgccgc aacggcattg acgttactgc cttccagaac
    74761 ccccgacatc acacgcaagg ttggatctgc ctgcagtacc ggccctcgcg tggcctgggt
    74821 ttctgcgctt aaacgaaaaa tgccgtcgtc accgcgctgc acttcgctgc ccgtggcttt
    74881 caccagtttc agacgcccta ctggcgcaac tgtatttgcc ggatcgcccg gattgagcgc
    74941 cgagattgtg ccatcggcag caatcgtgat ttccgcccct tccggcacag caattggccc
    75001 cgcctcgcct atcaccggat gcccctgaat tgtcagttgc ccggtgggat caacctgaat
    75061 gctgccatta cgcgtatacc cttcgctgcc gtcagcggtc tgcacggcca gccatcctgc
    75121 tgcaacgcaa cgtccagcgg gcgagaggtg taatccatct tgccgggcgt catgtctgca
    75181 cccggtgttg acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc caccggcact
    75241 gcgcgcaagg cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc cagattactg
    75301 gcggttaccg cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg
    75361 tgatccattg agctatcccg tcagcgatta gcgtaagtta acccgcgtgt tgaggatctg
    75421 gtcctgggtt ttgatggtct gggcgttaga cttatagtta cgctgggcaa cgatcatatt
    75481 gaccagttct ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagagtgcc
    75541 aaagtttccc gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca
    75601 gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg
    75661 ccccagcagt tgggtttgtt cgttggaatt gttgccgaca accgtaccgt catcattgat
    75721 ttgataactc accagatcgc ccggtttgta gccattctgg gtggttgcca caatgttgtt
    75781 agcgccggtt gcgatattat tcgccatcgc accatccact aatgtgccat tagcattaaa
    75841 ttccagtgtt gtcgctgtct tcgcaatgct gtttggatca ctgctatcct gggtgtagac
    75901 ctgccagtta ttatccccgg tcttcacaaa atagacgctc atgtcatgag cattaccctg
    75961 actgtcgaaa acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa
    76021 tggcgtaaca gtaggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt
    76081 ggtagttttc gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg
    76141 ctgaatagtc ggcggcgtac cggttaccgg gtaacccgtc agctgtaaac cttgcgtatt
    76201 caacaggtta cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc
    76261 gttgctgtct accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc
    76321 ggtgttggtg gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag
    76381 tcccactttc gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta
    76441 ggtggcggag ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa
    76501 tccgctaacc gcttgagaaa aggccatgac tgactcctga aatgtgaagg cttaaattat
    76561 ctgccgtact tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct
    76621 gttgccgcgg atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt
    76681 accaccgtta ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccatc
    76741 agtcaacgaa ccgtcccagg tgaaactgtg aactccggcg gtcagttcac caatatcaat
    76801 ggtgcgcaca accgcgccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg
    76861 ttgcagctca acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc
    76921 cgcaagaaca gttgtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa
    76981 cgactggctg ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat
    77041 cccgctgacc gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt
    77101 ggtcgggtcc tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc
    77161 tgcggcgttg ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg
    77221 atcggtggtg gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt
    77281 ttcagcatca tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac
    77341 gccgacatgg tgttaaccat ctccccgacg acatcaacgt tcggcatttt tatgtagccc
    77401 tttgcatctg ccagcggatt acctggttca taaaccagtt tgtccggggc ctgactttct
    77461 taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata
    77521 ccacctgttt tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca
    77581 gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat
    77641 caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt
    77701 tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact
    77761 gggtgcgttc gcgatccata tcgacggtat taccgtcaag cgaaggctgg tccggaatac
    77821 ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc cgggatgtgc tgcgttgagg
    77881 tcatcgtcag tgcaaccaca ctggttgcat cccgtccacg ttgcatgact tttttaagtt
    77941 cactggcaaa atcgatatcg cgcgcctgat aaccaggggt atcggcattg gcgatgtttg
    78001 ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga aaacgtaagg
    78061 cggcgtcgag cttatcgagc atatctcctc cgcaggtatc aaaattctgc catcagctta
    78121 aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct atttatgccg
    78181 ttgatggtca ttgcggacag gtacaattca cgttgtagaa atggctgggg gcgaaaatgc
    78241 tgacaataaa acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta agtacggcga
    78301 gcaatctcac atcgcaattg cacaactttt ttagcgccca actcgcgggg gtaagtgatg
    78361 aggttcgtgt ttctattcgt acagcgccca atctactacc gccatgcgag cagccattgc
    78421 tttcgatgag caataattcc cgcctgtggg gcaatgtgaa tgtgttggca cgctgcggta
    78481 acgacaaacg atatttacag gttaatgtgc aggccacagg aaattatgtg gttgccgcga
    78541 tgcctattgt gcggggagga aagctggaag ctggcaatgt caaactgaaa cgtggacgcc
    78601 tggataccct gccaccgcgt acggtgctgg atatcaatca acttgttgat gccgttagcc
    78661 tgcgcgatct atcacccgat caacctatcc agttaaccca gtttcgccag gcatggcggg
    78721 taaaagcggg acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc gccaacgcag
    78781 aaggtcaggc gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc atggtatcgg
    78841 aacaggtagt cagcggcgtt gttgatgcag atgggaatat tcttataaac ctgtaagctg
    78901 ttaaagatta cccgtccctt gccgataaat aagcaacaca tgataaaagc gccctcaatg
    78961 aggaataaac catgagtatt gatcgcactt cgcctctgaa gcctgtaagc accgttcaac
    79021 cgcgcgaaac caccgacgcg ccggtaacga acagccgggc ggcaaaaaca accgcctcca
    79081 ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc ggcagcagtg
    79141 atatcaatct tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt gaactaaaaa
    79201 tggacaccgg caaaattgcc gatgcgctga tcaacgaagc gcagcaagac ttgcagagta
    79261 actgatcgta tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt gcttaacgat
    79321 ctcaaaacgg taatggatca agagcagcaa catctctcta tggggcagat caacggcagc
    79381 cagttgcaat ggattacaga acaaaaaagc tcactgctgg cgacgctgga ttacctcgaa
    79441 cagttacgca agaaagaacc caatacagca aatagcgttg atattagtca acgctggcag
    79501 gaaattactg tgaaaacgca gcaactacgc caaatgaatc aacataacgg ctggttactg
    79561 gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca tcaggaaccg
    79621 acgctatatg gggcgaacgg tcagacctca acaccccatc gcggcagtaa aaagatttcg
    79681 atctgaatat tatctccggc ctgcactgca ggccggaatg cattgttaca ccgtccggcg
    79741 ggcaaattct ttaactttga agcccagtac cgccagtgca gcgaagtacg cggcaatccc
    79801 cgccagcacg accgccatta aacgcagtaa gcgccagggc atggtaccca atgaccactc
    79861 cggcatgata tgtaacatac ctaaaagcac gccagacatc accagtaccg ccaccaccag
    79921 acgcaacaga aacgccatcc agccgggttg cggagtaaag attttctgct tacgcaactg
    79981 ccagtaaagc agcgaagcat tcagacacgc cgccagacca atagaaagtg acagcccggc
    80041 atgtttcaac ggaccaataa acgccaggtt catcaattgc gttaaaatca gcgtaacgat
    80101 ggcaattttc actggcgttt taatgtcctg gcgggaataa aagccaggag ccaacacttt
    80161 cactacaatc aggccaatca aacccaccga gtaggcaatt aacgcccgct gggtcatcag
    80221 cgcatcaaac gcggtaaatt taccgtactg gaacagcgaa acggttaacg gacctgaaag
    80281 aatgcccaac gcaaccgcac tcggcagtgc caacaggaaa caaagacgca acccccagtc
    80341 catcaaacgg ttgtattcat catgattgcc actggcaaaa cttttcgaca gcgacggcag
    80401 caaaatggtg ccaagcgcca cgcccagcac accggaggga aactccatta agcggtcggc
    80461 gtaatacatc caagacaccg aaccggaagc aagaaacgag gcaaaaatgg tgttgatgat
    80521 taaggagatc tggctcacag agacgccaag gatcgccggt cccatctgtt tcaccacgcg
    80581 cattgctcca gcatcgtgga agttaatgcg cggcaggacc agcatgccga tcttcttcag
    80641 gtgcggtagc tgatacacca gctgcagaat gccgccgacc gtaacagccc acgccagcgc
    80701 cagcaccggt gggttaaagt acggtgcggc aaacagcgcg aaaccaatca tgctgatgtt
    80761 aagcagtgtt ggcgcaaacg ccggaatcga gaagcggttc cacgtattca gaatcgctcc
    80821 caccagcgac gccagggaga tcagcaagat atagggaaag gtaatcttta gtagctggct
    80881 ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca tgatcaccca
    80941 cggtgcggcg agcatgccag cgaccgtcac aaccgccagc gcaagtgtca gcaggccaga
    81001 aacataagag acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat attccgccag
    81061 aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag atacggcgta acaagttagg
    81121 aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga ttctggcgac
    81181 aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc tgacggcggc
    81241 cagcgatttt aataaattca tcggtgttct aatccggacc cacgggcaaa acgcctgcaa
    81301 ggcaggcgct gaaagaggcg aatagtctac ctagattatg gtgaattgct accgccagat
    81361 gttacagggt ttattcactc atcgcatcgc gccagatctt gtcaacgata cgttgcgcca
    81421 gcactgccta ttcgccggcg gtttgcggaa ctgtctgatt ttacacgcat tcaatgaagt
    81481 gccgcgcaca gccgacaaat ccacgttgct caagagtgct ctgccaacca ggaatcggtt
    81541 tattcactac accctgcccg cgctcctcac gccattcgcg catatccgta atgtcgatga
    81601 gcgcaccgtc agtcacggcc tgcacggttt cacgctgact tccggcacgg cgatgcatac
    81661 aagtggtgat ctgcaaagga ccggccgaaa aatggtgctc ggcaaacagc atttcgccag
    81721 cgtcgttagt cagtagcgta ccgccatcca gagaggcttt gccgcccgac aaccacagcg
    81781 cggtatccac cacatgcaga taatcatcca gcaacgtgaa ataaagatcg tgtggcccga
    81841 cgctattgct acgatgttta tccattctta gcgaggctgc ggtggcgagt tgcgttttta
    81901 actcaccgta gagtggtgcg aaacgacggt taaaaccgac catcaacgtc agttttttcc
    81961 gcgccgccag ttccaccagc ctttcagcat cgcacagatt ttctgccagc ggtttatcga
    82021 cacagacatg tacccccgca ttgagtaacg tactgaccac gtcaaagtgg ctggcggtgc
    82081 tggagtgcac aaaaaccgca tcacaactgg cggcaaggct ggataacgaa tcggcataag
    82141 gaatgcacca gctgtcacaa attggcaggg ctttggcgcg cgtaggcgac caggctcctt
    82201 gtaacgtcca gtcagacgct gccgccagca ccggtaacca cgctttttgc gcaatgccgc
    82261 ctaatcccac tacgccgata cgtaattttt tcactttaat ctcccagatg ggccagcaac
    82321 gaatcaagac gttgtttcag ttctgccact tcgatttcca gggcttcgac gcgggtctgt
    82381 aaatcaccgt caaccatgtt cgacatagcc gttaccgccg gctgatcttc aacctcacca
    82441 ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag acgcaccaca
    82501 aaaggaccat cttcgcgatt cgccagttgt tccagcgtcg actccacttc cgccatatcg
    82561 ctgaattcat acattcgcgc ggcgcggctg cgcagttcac ccggcgtctg ggcaccacgc
    82621 aataacaacg tggtgattaa cgccacttct gcggcgctca gtttcagatc gccaaattct
    82681 gaattacaaa aacgttgctc atatttggtg acccgattac caaaaccgct cactgtgcgt
    82741 agataatgac gtttgaccag attatccagc tgttcctgca cttcggattc actcagattc
    82801 atgaccggtt cacggttcgt tttctgatta caggccgtga ctacaccatt gactgagagc
    82861 gggtattgct ccggcgtggt cacctgcttt tccagcaagc aaccaattac gcgagcttcc
    82921 agggcagtaa gttgatattt catgagattc tccttagcgg ccgggcgtcc agtctggggt
    82981 agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca atcaacagat agtctttcgc
    83041 atagccttct ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt tattgtgcgg
    83101 catataatta gccataatgc gatgaatatg ttgggtgcgc tgcatataac gaatggctgc
    83161 ggtcagggct tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg aataaccgag
    83221 atagcaggca tgaaaagagc cgcgaacaac attggaaaaa ttggcaacgc caataatctc
    83281 tttttcgtcc gggtcgaata agccaaagta gaaagctgaa ccttgtttat gaaattcgtt
    83341 aatcatccct agcctggcct gccagcctga tggataacag tggctttcgt cgcgcactgg
    83401 ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa tccgccagac gccaggcatc
    83461 acgatcatgc accagacgca cgaccagtcg gtctgtggtt aagcgcactt ttggcacgtt
    83521 actgcgatag ccaaacattc tatacctact ccttcccgta acgtctttaa tacgctgtaa
    83581 gtattactat acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat tttctgcatt
    83641 attcacatat ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa aatactgtcc
    83701 ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca tcttttattt
    83761 cccgggaggg gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat atttcctgct
    83821 catcgataat atgctggtcg tgctggggtt ctttgttgtc ttcccgctga tctctatccg
    83881 cttcgttgat caaatgggct gggccgccgt catggtcggt attgctctcg gtctacgcca
    83941 gtttattcag caaggtctgg gtattttcgg cggcgcaatt gccgaccgct ttggtgccaa
    84001 accgatgatt gttaccggta tgctgatgcg cgccgccgga ttcgccacaa tgggtatcgc
    84061 ccacgaaccg tggctattgt ggttttcatg cctgctctcg ggactcggcg gcacgttgtt
    84121 tgatccgccg cgttcggcgc tggtggtgaa actaatccgt ccacaacaac gtggtcgttt
    84181 tttctcgctg ttgatgatgc aggacagtgc cagtgcggtc attggcgcat tgttggggag
    84241 ctggctgttg caatacgact ttcgcctggt ctgcgccaca ggggccgttt tgtttgtgct
    84301 atgtgcggcg ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg tacgcacgcc
    84361 cgttcgcgaa ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct atgtcctgac
    84421 gctggcgggt tactacatgc tggctgtaca agtgatgctg atgctgccaa ttatggtcaa
    84481 cgacgtggct ggcgcgccct ctgccgttaa atggatgtat gccattgaag cgtgtctgtc
    84541 gttaacgttg ctctacccta tcgcccgctg gagtgaaaag cattttcgtc tggaacaccg
    84601 gttgatggct gggctgttga taatgtcatt aagcatgatg ccggtgggca tggtcagcgg
    84661 cctacaacaa cttttcaccc tgatttgtct gttttatatc gggtcgatca ttgccgagcc
    84721 tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga gctcgcggca gctatatggg
    84781 gtttagccgt ctgggtctgg cgattggcgg cgctattggt tatatcggtg gtggctggct
    84841 gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc tgggaattat
    84901 tggcatcttc actttccttg cgctgggttg gcagtttagt cagaaacgcg ccgcgcgtcg
    84961 tttgctggaa cgcgacgcct gatttgatac tcccccctga ctcctccata ctgaaagtga
    85021 actgacagcc aaatggagga gtcacgtgaa gctatacatt tacgatcact gcccttactg
    85081 cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc gtcgaattac atgttctgct
    85141 caacgacgac gcagaaacac ccacccggat ggtcggtcaa aaacaggttc ccattctgca
    85201 aaaagatgac agccgctata tgccagaaag catggatatc gttcactatg tcgataaact
    85261 cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc attgaagagt ggctgcgcaa
    85321 ggtcaatggc tacgtcaaca aactgctgtt gccgcgtttt gccaaatcgg catttgatga
    85381 gttttctact cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca gcgcgggtaa
    85441 ttttgccgac ctgctggccc actctgacgg tctgattaag aatatcagcg atgatttgcg
    85501 tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt cggaagatga
    85561 tattcagcta tttccgctac tgcgtaatct gacgctggta gccggaatca actggccaag
    85621 ccgcgttgct aattaccgcg ataatatggc gaaacagaca caaatcaatt tgttatcatc
    85681 aatggcgatt taatcctgac ccgccgcggc agcctgtatt cgcgggttgc cgccgttttc
    85741 agttttttct gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt caggatattg
    85801 acgcagattt catgtcgagg actaccatga acaagttttt atttgccgct gcattgatcg
    85861 tcagtggcct gctcgttggc tgtaatcaac tcacccaata caccatcacc gaacaagaaa
    85921 ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga tatcggttta cccggcgtgg
    85981 ctgacgccca tattgttctg acaaacttga ccagccaaat tggtcgcgaa gagccgaata
    86041 aggttaccct aaccggagac gccaatctgg acatgaactc cctgttcggt agtcagaaag
    86101 cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa ggtgcgatct
    86161 tcctgaaaga gatggaagtg gtcgatgcga cggtacaacc ggaaaaaatg caaacggtga
    86221 tgcaaacgtt gcttccctat ttgaaccagg cattacgcaa ttactttaac cagcaacctg
    86281 cttacgtcct gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa ctggcgaaag
    86341 gcattgaagt gaagccaggc gaaattgtca ttccgtttac ggattaatga cgagggcgca
    86401 ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg cttatccttt gtgtccggca
    86461 aaaacatccc ttcagccgga gcatagagat taatgactgc accatcccag gtattaaaga
    86521 tccgccgccc agacgactgg caccttcacc tccgcgatgg cgacatgtta aaaactgtcg
    86581 tgccgtatac cagcgaaatt tatggacggg ctatcgtaat gcccaatctg gctccgcccg
    86641 tgaccacagt tgaggctgcc gtggcgtatc gccaacgtat tcttgacgcc gtacctgccg
    86701 ggcacgattt caccccattg atgacctgtt atttaacaga ttcgctggat cctaatgagc
    86761 tggagcgcgg atttaatgaa gccgtgttca ccgctgcaaa actttacccg gcaaacgcaa
    86821 ccactaactc cagccacggc gtgacgtcaa ttgacgcaat catgccggta cttgagcgca
    86881 tggaaaaaat cggtatgccg ctactggtgc atggtgaagt gacacatgca gatatcgaca
    86941 tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc cagcgcctga
    87001 ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga tgctgccgac tatgtccgtg
    87061 acggaaatga acggctggct gccaccatca ctccgcagca tctgatgttt aaccgcaacc
    87121 atatgctggt tggtggcgtg cgtccgcacc tgtattgttt acccatcctc aaacgcaata
    87181 ttcaccaaca ggcattgcgt gaactggtcg ccagcggttt taatcgagta ttcctcggta
    87241 cggattctgc gccacatgca cgtcatcgca aagagagcag ttgcggctgc gcgggctgct
    87301 tcaacgcccc aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg aatgctctgc
    87361 agcactttga agcattctgt tctgtaaacg gcccgcagtt ctatggcttg ccggtcaacg
    87421 acacattcat cgaactggta cgtgaagagc aacaggttgc tgaaagcatc gcactgactg
    87481 atgacaccct ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt aaacaataaa
    87541 aatcgtagct tcctgttgtc attaggttat tttacctgta taaataacca gtatattcaa
    87601 cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt gccagctggg
    87661 gctattgacg ccctggctgg cgaactttcc cgccgtattc agtatgcgtt tcctgataat
    87721 gaaggccacg tatcggtacg ttatgccgca gcgaataatt tatcggttat tggcgcaaca
    87781 aaagaagata aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag cgccgatgac
    87841 tggtttgtca gcgaataata tgcagtgatt tttgtttttg ccggatcgcc ccggcttttt
    87901 tattttactt acgaattttg agtacaaaat ttctacactt cctcaaaata attgcacaat
    87961 atgctaaaaa aatatgaatg acatgctgtt ttattgttca tataaatctt aaaagcagaa
    88021 atttatctgt tccccccttt aaaaattcgg tgaataccct tacttattgg tatactgaag
    88081 ttgcttcaga taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg
    88141 ggccatgatg gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat
    88201 cagcaccgtt gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa
    88261 gtccgagcag gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag
    88321 acaatttatt tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt
    88381 aaacgagtat cggcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt
    88441 gcctttacca tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga
    88501 gagcagagag aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc
    88561 tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg acgcccatat
    88621 gccaccgcat caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg
    88681 tgaaggcgaa aagtatgtcc cgttggtcct ccgcgcgcaa acgctgtggg atgaactttc
    88741 ccgccacaac gaagatgatc ccatttttgt acgctctggt gtcattaacc tcggcccggc
    88801 tgactccgca tttctcgcca acgtcgccca cagcgccgaa cagtggcaac tcaacgttga
    88861 aaagctcgat gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta
    88921 catcggctta tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg
    88981 gatccaactg gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg tcaccgcaat
    89041 tcgtcatgac gatgatggcg taactattga aacggttgac ggtgagtatc aggcgaaaaa
    89101 agcgattgtc tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc
    89161 cgtacgtaaa gtatttgcct ggtatcaggc cgatggccgc tatagcgtga agaataaatt
    89221 cccggcgttt accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa
    89281 cgacgcgttg aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg
    89341 tgttccgttt gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct tgcgcaatgt
    89401 attgccgggt atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga
    89461 cgaagatttt attatcgata ccctacccgg ccacgataat acactgctca ttaccggcct
    89521 gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca
    89581 agaccaaaaa agcgattttg atttgacgcc attcaggctt tcccgcttcc aataatcatc
    89641 attacggcct ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac
    89701 tatctcatca ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg
    89761 gcaattatgg atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac
    89821 aaatatttaa tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta
    89881 cagcactcac cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta
    89941 ttaattcaaa attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca
    90001 attcattctc cgacacgcca ttttctttct ctcgcaaatt cgtattggat gcacttatgt
    90061 cattcacaaa tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg
    90121 caattattgt ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg
    90181 atggctggta tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg
    90241 ggctggttat tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt
    90301 attcgcccat gactcgtctt gccgcaaaag ctggacatct cgcattgtgc ctattgctgt
    90361 tcgccatcgg catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt
    90421 ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg
    90481 gtgccctgca tttttggctc gcgtggagcg tcgtagtact gtctgttatg cacggattta
    90541 tggccctgaa gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt
    90601 cgtcatctga ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt
    90661 ccctgatgtt ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc
    90721 agcacgcctt tgttaacttc cgcatacagc atcttggcta tagctggtta tatggcacct
    90781 ttaaagattt cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga
    90841 atgtgacaat taacaccacc agcgtcgata ctaaccacgc cgaacgcgat aaacatcttc
    90901 gcagtgcgga ttttctcaat accacaaaat atccacaggc aacattcacc tccaccagcg
    90961 tgaagaaaga cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca
    91021 aacccgtcac gctggaagcg aaattaattg gtcagggcga cgacccatgg ggcggtaaac
    91081 gtgcaggctt tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt
    91141 taggtccagc ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt
    91201 aagagtgaaa tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag
    91261 gaatacccag tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt
    91321 cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa
    91381 tctcactaca acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt
    91441 ggcagccatc atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat
    91501 gcgcgataat ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc
    91561 caataaaacg caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc
    91621 cctcgatatg ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac
    91681 aacgaatgcc gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct
    91741 cgactgcttt tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa
    91801 agtgccccac ttcatactca tagtggttac gcatgtcgat aaatagtgca tcgggatcgt
    91861 caagcatggc gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt
    91921 gaggatcgtc aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga
    91981 aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga
    92041 aggcatagag ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt
    92101 taatgccctc atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat
    92161 ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg
    92221 tggttcgcgg ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt
    92281 tgtgtaacac tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat
    92341 gatatagcaa ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa
    92401 attatttgtt atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg
    92461 cgggcgaaaa aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat
    92521 atgacgaatc tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg
    92581 ttgggtattg gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc
    92641 ggttgtggat taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat
    92701 cgcaacctgg aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg
    92761 aagaatttcg aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg
    92821 gaccgccgaa tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag
    92881 gcgcaaaaac gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg
    92941 cggcagtttg gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg
    93001 attgactggc tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa
    93061 gatttaaaag gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccagat
    93121 catgattacg gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg
    93181 accacgaccg gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt
    93241 ccacgccgta agccagatgg caaagggtat caattgatta tgctgccacc agagtgttct
    93301 ccgccactgg atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc
    93361 atcatgatgg caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa
    93421 ggcgttcctt cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt
    93481 accacagcaa atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt
    93541 cgggcgcata attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa
    93601 cagcggattg ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg
    93661 atcgtcgcct ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc
    93721 ttacccctct acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc
    93781 ggtattgtct tcagtataat gggttcctaa acacatcatt ttaggtattg aatatgagac
    93841 gggcaatcag ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc
    93901 gacaatctaa gctggttact gactggcttg ataagaaccc tgagttttac ttggattcat
    93961 ccttgagctt taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc
    94021 taggtgactt tttaaccgct atcgagaaag ggcttgttaa agctggtgat acgctactaa
    94081 tcgagagttt agaccgtctg tcacgtcagg atattgatat tgcttctgag ctattacgcc
    94141 gtatcttaag ggctggtgta gatgtagtca cactgagcga tggagaacat tacacacgag
    94201 aaagtttaaa agacccgtta gccctgatta aaagcattct gataatgcag cgggcgcatg
    94261 aggaaagttt gagaaagtct gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca
    94321 tctccgaagg tatcaaggta tctaggcgtt gtcctgcatg gttaaggctg aatgatgacc
    94381 gtcgaacatt cacaatcatt cctgataaag tggaagttgt taaacgagca tttgacctga
    94441 gattacaggg tttaagcttt tgggctatca ccagaacttt aaa
//
LOCUS       DAJTYH010000002        83235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTYH010000002 DAJTYH010000000
VERSION     DAJTYH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 83235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            220..579
                     /locus_tag="OO609_000106"
     CDS             220..579
                     /locus_tag="OO609_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6631668.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            581..973
                     /locus_tag="OO609_000107"
     CDS             581..973
                     /locus_tag="OO609_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6631669.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(1025..2440)
                     /gene="gltX"
                     /locus_tag="OO609_000108"
     CDS             complement(1025..2440)
                     /gene="gltX"
                     /locus_tag="OO609_000108"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6631670.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            2699..2774
                     /locus_tag="OO609_000109"
     tRNA            2699..2774
                     /locus_tag="OO609_000109"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2732..2734,aa:Val,seq:tac)
     gene            2819..2894
                     /locus_tag="OO609_000110"
     tRNA            2819..2894
                     /locus_tag="OO609_000110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Val,seq:tac)
     gene            2899..2974
                     /locus_tag="OO609_000111"
     tRNA            2899..2974
                     /locus_tag="OO609_000111"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2932..2934,aa:Lys,seq:ttt)
     gene            3005..3130
                     /locus_tag="OO609_000112"
     ncRNA           3005..3130
                     /ncRNA_class="other"
                     /locus_tag="OO609_000112"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            3250..3591
                     /locus_tag="OO609_000113"
     CDS             3250..3591
                     /locus_tag="OO609_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6631671.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(3582..4508)
                     /locus_tag="OO609_000114"
     CDS             complement(3582..4508)
                     /locus_tag="OO609_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6631672.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4598..5596
                     /locus_tag="OO609_000115"
     CDS             4598..5596
                     /locus_tag="OO609_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6631673.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5593..5811)
                     /locus_tag="OO609_000116"
     CDS             complement(5593..5811)
                     /locus_tag="OO609_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6631674.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5813..7828)
                     /gene="ligA"
                     /locus_tag="OO609_000117"
     CDS             complement(5813..7828)
                     /gene="ligA"
                     /locus_tag="OO609_000117"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6631675.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(7899..8885)
                     /gene="zipA"
                     /locus_tag="OO609_000118"
     CDS             complement(7899..8885)
                     /gene="zipA"
                     /locus_tag="OO609_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6631676.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9115..9876
                     /gene="cysZ"
                     /locus_tag="OO609_000119"
     CDS             9115..9876
                     /gene="cysZ"
                     /locus_tag="OO609_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6631677.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10061..11032
                     /gene="cysK"
                     /locus_tag="OO609_000120"
     CDS             10061..11032
                     /gene="cysK"
                     /locus_tag="OO609_000120"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6631678.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11416..11673
                     /gene="ptsH"
                     /locus_tag="OO609_000121"
     CDS             11416..11673
                     /gene="ptsH"
                     /locus_tag="OO609_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6631679.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11718..13445
                     /gene="ptsI"
                     /locus_tag="OO609_000122"
     CDS             11718..13445
                     /gene="ptsI"
                     /locus_tag="OO609_000122"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6631680.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13486..13995
                     /gene="crr"
                     /locus_tag="OO609_000123"
     CDS             13486..13995
                     /gene="crr"
                     /locus_tag="OO609_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6631681.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(14037..14888)
                     /gene="pdxK"
                     /locus_tag="OO609_000124"
                     /gene_synonym="thiJ"
     CDS             complement(14037..14888)
                     /gene="pdxK"
                     /locus_tag="OO609_000124"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6631682.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14993..15367
                     /locus_tag="OO609_000125"
     CDS             14993..15367
                     /locus_tag="OO609_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6631683.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            15400..16123
                     /locus_tag="OO609_000126"
                     /pseudo
     CDS             15400..16123
                     /locus_tag="OO609_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(16327..17238)
                     /gene="cysM"
                     /locus_tag="OO609_000127"
     CDS             complement(16327..17238)
                     /gene="cysM"
                     /locus_tag="OO609_000127"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6631684.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17372..18469)
                     /gene="cysA"
                     /locus_tag="OO609_000128"
     CDS             complement(17372..18469)
                     /gene="cysA"
                     /locus_tag="OO609_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6631685.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(18459..19334)
                     /gene="cysW"
                     /locus_tag="OO609_000129"
     CDS             complement(18459..19334)
                     /gene="cysW"
                     /locus_tag="OO609_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6631686.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(19334..20167)
                     /gene="cysT"
                     /locus_tag="OO609_000130"
     CDS             complement(19334..20167)
                     /gene="cysT"
                     /locus_tag="OO609_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6631687.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20167..21183)
                     /gene="cysP"
                     /locus_tag="OO609_000131"
     CDS             complement(20167..21183)
                     /gene="cysP"
                     /locus_tag="OO609_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6631688.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(21487..22278)
                     /gene="ucpA"
                     /locus_tag="OO609_000132"
     CDS             complement(21487..22278)
                     /gene="ucpA"
                     /locus_tag="OO609_000132"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6631689.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(22465..23265)
                     /gene="murR"
                     /locus_tag="OO609_000133"
     CDS             complement(22465..23265)
                     /gene="murR"
                     /locus_tag="OO609_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6631690.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            23429..24325
                     /gene="murQ"
                     /locus_tag="OO609_000134"
     CDS             23429..24325
                     /gene="murQ"
                     /locus_tag="OO609_000134"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6631691.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            24329..25753
                     /gene="murP"
                     /locus_tag="OO609_000135"
     CDS             24329..25753
                     /gene="murP"
                     /locus_tag="OO609_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6631692.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(25933..26832)
                     /gene="yfeX"
                     /locus_tag="OO609_000136"
     CDS             complement(25933..26832)
                     /gene="yfeX"
                     /locus_tag="OO609_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6631693.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26928..27503)
                     /locus_tag="OO609_000137"
     CDS             complement(26928..27503)
                     /locus_tag="OO609_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6631694.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27564..28013)
                     /locus_tag="OO609_000138"
     CDS             complement(27564..28013)
                     /locus_tag="OO609_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6631695.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(28000..28425)
                     /locus_tag="OO609_000139"
     CDS             complement(28000..28425)
                     /locus_tag="OO609_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6631696.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28639..29508
                     /gene="amiA"
                     /locus_tag="OO609_000140"
     CDS             28639..29508
                     /gene="amiA"
                     /locus_tag="OO609_000140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6631697.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29512..30411
                     /gene="hemF"
                     /locus_tag="OO609_000141"
     CDS             29512..30411
                     /gene="hemF"
                     /locus_tag="OO609_000141"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6631698.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30417..31469)
                     /gene="eutR"
                     /locus_tag="OO609_000142"
                     /pseudo
     CDS             complement(30417..31469)
                     /gene="eutR"
                     /locus_tag="OO609_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(31515..32015)
                     /gene="eutK"
                     /locus_tag="OO609_000143"
     CDS             complement(31515..32015)
                     /gene="eutK"
                     /locus_tag="OO609_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6631699.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(32028..32687)
                     /gene="eutL"
                     /locus_tag="OO609_000144"
     CDS             complement(32028..32687)
                     /gene="eutL"
                     /locus_tag="OO609_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6631700.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            33869..34660
                     /locus_tag="OO609_000145"
     CDS             33869..34660
                     /locus_tag="OO609_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631701.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            35380..35775
                     /locus_tag="OO609_000146"
     CDS             35380..35775
                     /locus_tag="OO609_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6631702.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            35793..36056
                     /locus_tag="OO609_000147"
     CDS             35793..36056
                     /locus_tag="OO609_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631703.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            36079..36621
                     /locus_tag="OO609_000148"
     CDS             36079..36621
                     /locus_tag="OO609_000148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631704.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            36760..37047
                     /locus_tag="OO609_000149"
     CDS             36760..37047
                     /locus_tag="OO609_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631705.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            37086..37292
                     /locus_tag="OO609_000150"
     CDS             37086..37292
                     /locus_tag="OO609_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631706.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            37604..38089
                     /locus_tag="OO609_000151"
     CDS             37604..38089
                     /locus_tag="OO609_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6631707.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            38070..38348
                     /locus_tag="OO609_000152"
     CDS             38070..38348
                     /locus_tag="OO609_000152"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631708.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            38341..39801
                     /locus_tag="OO609_000153"
     CDS             38341..39801
                     /locus_tag="OO609_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6631709.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            39835..40644
                     /gene="murI"
                     /locus_tag="OO609_000154"
     CDS             39835..40644
                     /gene="murI"
                     /locus_tag="OO609_000154"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6631710.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(40763..41457)
                     /locus_tag="OO609_000155"
     CDS             complement(join(40763..41211,41211..41457))
                     /locus_tag="OO609_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6631711.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(41976..43349)
                     /gene="eutA"
                     /locus_tag="OO609_000156"
     CDS             complement(41976..43349)
                     /gene="eutA"
                     /locus_tag="OO609_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6631712.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(43324..43803)
                     /gene="eutP"
                     /locus_tag="OO609_000157"
     CDS             complement(43324..43803)
                     /gene="eutP"
                     /locus_tag="OO609_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6631713.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(43816..44151)
                     /gene="eutS"
                     /locus_tag="OO609_000158"
     CDS             complement(43816..44151)
                     /gene="eutS"
                     /locus_tag="OO609_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6631714.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(44444..46723)
                     /gene="maeB"
                     /locus_tag="OO609_000159"
     CDS             complement(44444..46723)
                     /gene="maeB"
                     /locus_tag="OO609_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6631715.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            47012..47962
                     /gene="tal"
                     /locus_tag="OO609_000160"
     CDS             47012..47962
                     /gene="tal"
                     /locus_tag="OO609_000160"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6631716.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            47982..49985
                     /gene="tkt"
                     /locus_tag="OO609_000161"
     CDS             47982..49985
                     /gene="tkt"
                     /locus_tag="OO609_000161"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6631717.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(50088..51131)
                     /locus_tag="OO609_000162"
     CDS             complement(50088..51131)
                     /locus_tag="OO609_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6631718.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(51257..51832)
                     /gene="nudK"
                     /locus_tag="OO609_000163"
     CDS             complement(51257..51832)
                     /gene="nudK"
                     /locus_tag="OO609_000163"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6631719.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(51900..53879)
                     /gene="aegA"
                     /locus_tag="OO609_000164"
     CDS             complement(51900..53879)
                     /gene="aegA"
                     /locus_tag="OO609_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6631720.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            54085..55802
                     /gene="narQ"
                     /locus_tag="OO609_000165"
                     /pseudo
     CDS             54085..55802
                     /gene="narQ"
                     /locus_tag="OO609_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            56004..59117
                     /gene="acrD"
                     /locus_tag="OO609_000166"
     CDS             56004..59117
                     /gene="acrD"
                     /locus_tag="OO609_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6631721.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(59216..59275)
                     /gene="ypfM"
                     /locus_tag="OO609_000167"
     CDS             complement(59216..59275)
                     /gene="ypfM"
                     /locus_tag="OO609_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6631722.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            59656..60012
                     /locus_tag="OO609_000168"
     CDS             59656..60012
                     /locus_tag="OO609_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6631723.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            60016..61143
                     /gene="dapE"
                     /locus_tag="OO609_000169"
     CDS             60016..61143
                     /gene="dapE"
                     /locus_tag="OO609_000169"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6631724.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            61171..61371
                     /locus_tag="OO609_000170"
     CDS             61171..61371
                     /locus_tag="OO609_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6631725.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(61481..62179)
                     /gene="ypfH"
                     /locus_tag="OO609_000171"
     CDS             complement(61481..62179)
                     /gene="ypfH"
                     /locus_tag="OO609_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6631726.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(62253..64268)
                     /gene="tmcA"
                     /locus_tag="OO609_000172"
     CDS             complement(62253..64268)
                     /gene="tmcA"
                     /locus_tag="OO609_000172"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6631727.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(64283..65146)
                     /locus_tag="OO609_000173"
     CDS             complement(64283..65146)
                     /locus_tag="OO609_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6631728.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(65314..66027)
                     /gene="purC"
                     /locus_tag="OO609_000174"
     CDS             complement(65314..66027)
                     /gene="purC"
                     /locus_tag="OO609_000174"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6631729.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(66240..67274)
                     /gene="bamC"
                     /locus_tag="OO609_000175"
     CDS             complement(66240..67274)
                     /gene="bamC"
                     /locus_tag="OO609_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6631730.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(67291..68169)
                     /gene="dapA"
                     /locus_tag="OO609_000176"
     CDS             complement(67291..68169)
                     /gene="dapA"
                     /locus_tag="OO609_000176"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6631731.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            68315..68887
                     /locus_tag="OO609_000177"
     CDS             68315..68887
                     /locus_tag="OO609_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6631732.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            68887..69357
                     /gene="bcp"
                     /locus_tag="OO609_000178"
     CDS             68887..69357
                     /gene="bcp"
                     /locus_tag="OO609_000178"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6631733.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            69610..70227
                     /gene="hyfA"
                     /locus_tag="OO609_000179"
     CDS             69610..70227
                     /gene="hyfA"
                     /locus_tag="OO609_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6631734.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            70227..72244
                     /gene="hyfB"
                     /locus_tag="OO609_000180"
                     /pseudo
     CDS             70227..72244
                     /gene="hyfB"
                     /locus_tag="OO609_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            72255..73202
                     /gene="hyfC"
                     /locus_tag="OO609_000181"
     CDS             72255..73202
                     /gene="hyfC"
                     /locus_tag="OO609_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6631735.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            73219..74658
                     /gene="hyfD"
                     /locus_tag="OO609_000182"
     CDS             73219..74658
                     /gene="hyfD"
                     /locus_tag="OO609_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6631736.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            74670..75320
                     /gene="hyfE"
                     /locus_tag="OO609_000183"
     CDS             74670..75320
                     /gene="hyfE"
                     /locus_tag="OO609_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6631737.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            75325..76911
                     /gene="hyfF"
                     /locus_tag="OO609_000184"
     CDS             75325..76911
                     /gene="hyfF"
                     /locus_tag="OO609_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6631738.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            76901..78615
                     /gene="hyfG"
                     /locus_tag="OO609_000185"
                     /pseudo
     CDS             76901..78615
                     /gene="hyfG"
                     /locus_tag="OO609_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            78625..79170
                     /gene="hyfH"
                     /locus_tag="OO609_000186"
     CDS             78625..79170
                     /gene="hyfH"
                     /locus_tag="OO609_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6631739.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            79167..79925
                     /gene="hyfI"
                     /locus_tag="OO609_000187"
     CDS             79167..79925
                     /gene="hyfI"
                     /locus_tag="OO609_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6631740.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            79918..80331
                     /gene="hyfJ"
                     /locus_tag="OO609_000188"
     CDS             79918..80331
                     /gene="hyfJ"
                     /locus_tag="OO609_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6631741.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            80361..82190
                     /gene="hyfR"
                     /locus_tag="OO609_000189"
     CDS             80361..82190
                     /gene="hyfR"
                     /locus_tag="OO609_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6631742.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            82191..82373
                     /locus_tag="OO609_000190"
     CDS             82191..82373
                     /locus_tag="OO609_000190"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR6631743.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            82395..83235
                     /locus_tag="OO609_000191"
                     /pseudo
     CDS             82395..83235
                     /locus_tag="OO609_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
BASE COUNT        19974 a        21726 c        20961 g        20574 t
ORIGIN      
        1 tcacgtaaag cttgtcgagt tgacgggcgg catcatatga attccgcccg aatgtgtcaa
       61 cggcaaattg caacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga
      121 aaaccgctcg cattcagtag ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa
      181 tgaactacta atagacccac atacattcag ggaattgtta tgttcaagga gaggatgaca
      241 ccagatgaac ttgccagact gaccggctat agccgccaga ccattaataa atgggtacgc
      301 aaggaaggct ggacaacgtc accaaaacct ggcgtccagg gtggcaaagc ccgactggtt
      361 cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg aacgtccaga aggtcaggga
      421 gaagcgcctg ccctttctgg tgatgcgccg cttgaagtat tgctggtgac actggcgaaa
      481 gagatgacgc cagttgaaca aaaacagttt acatccttgc ttctgcggga agggattatc
      541 ggattgttac aacgcttagg gattcgcgat agcaaataat atgaaaagat tacgcaataa
      601 aatgaccacc gaagaactgg ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg
      661 gaccagagaa aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag
      721 acttattctg gtcgatacgc aagtttgcga gtttattcag aacacgccag ccttccataa
      781 cacaccaatg ttgatggaag ctgaagagag aattgctgaa tacgctcctg gagcacgcgc
      841 cccggcgtat cgacaaatca tcaacgcgat agataatatg acagacattg agcaggaaaa
      901 agtcgcgcaa tttttgtcac gtgaaggaat tcgcaatttc ctcgcccgtc tcgatattga
      961 cgaatcagca taaataaaaa acggcaggat attatctcct gccgtttatc tttttacacg
     1021 ctaattactg ctgattttcg cgttcagcaa taaaatccag cgctttgttg atacgctcga
     1081 tactgcgggt cttaccaatt gcgtgaacgg taacatccag tgctggagac tgccccgcac
     1141 cggttacggc gacacgcagc ggcataccaa ctttacccat acccacttcc agctcatccg
     1201 ccgtcgcctg aatagcgtga tgaacgtttt cagcggtcca gtcagtaatc gcggccagtt
     1261 tgtcacgaac cacttccagc ggctgacgcg ctaccggacg cagatgtttt ttcgccgcat
     1321 cggcatcgaa ctcagcaaaa tcttcgtaga aataacggca gctctgtgcc atctctttca
     1381 gcgtcttgca gcgttcgccc agcagtttca ccagatcagc cagctgcggg ccgttacggg
     1441 tatcgatatt ttcctgctca atgtgccact gtaagtgagt agcaacatac tccggcggca
     1501 gcgcgttaat gtagtgatgg ttcagccaca gcagcttgtc ggtgttgaac gcactggcag
     1561 atttgctgac ggcattcaaa gtgaagtatt tgatcatctc ttcacgagtg aagatttcct
     1621 gatcgccgtg ggaccagccc agacgcacca gatagttcag cagtgcttct ggcaaataac
     1681 cgtcatcacg atactgcatt acgctgactg ccccgtgacg tttggacagt tttttaccgt
     1741 catcgccatt gatcatagaa acgtgcgcgt aaaccggcac cggcgctttc agggccttaa
     1801 gaatgttgat ctggcgcggc gtgttgttga tatggtcttc gccacggata acgtgggtga
     1861 tttccatatc ccagtcatcg acaaccacac agaagttata ggttggggaa ccatcggtac
     1921 ggcggataat aagatcgtcc agttcctggt tgctgaactc gatcggacca cggatctgat
     1981 cgtcaaaaac aacagaacct tcctgcgggt tagcaaaacg tacaacacac ggttcatcat
     2041 cagcatgatg ctcatggctg tggcggcagc gaccgtcata acgcggcttc tcacctttcg
     2101 ccatttgctc ttcgcgcagc gcttccaggc gctctttaga gcaatagcat ttataagcag
     2161 tgccctcttc cagcatctga tcgatcaccg cgttgtagcg atcaaaacgt ttggtctggt
     2221 agtacggacc ttcatcccac tccaggctca gccagttcat gccatccata atggcttcga
     2281 tagcttccgg cgtggaacgc tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc
     2341 cgtggttacg tgcaaaaagc caggagtaaa gagcagtacg cgcgccgcca acgtgcagat
     2401 agcctgttgg gcttggcgcg aagcgagttt tgattttcat gaaatggcct tacgtttaga
     2461 aagatgccga caaccggcaa atcctggaaa aattgagtgg gcgatattct atcactcccg
     2521 cctgattcct caatgtagtt cgggtttcta ccttacgcat tattgttttc gttgacaaat
     2581 tgcgcaacac ggtctgtttt gcgatcatct tgcttaaatt tacgacgaac gaacaatttc
     2641 tttagaaaat gcgttgactc attttgaact ctccctataa tgcgactcca cacagcgggg
     2701 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
     2761 gtcatcaccc accaactact ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg
     2821 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
     2881 gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac ttttaatcaa
     2941 ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct
     3001 tttttgctat ctgcgatact caaagattcg aacctgcagc aggtttgagt tgagcgcagc
     3061 gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca
     3121 cgacccacca atgcaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatttgtaag
     3181 attgattttt cttctcaagt tccttgttct tctaccgata ttacctcgta cagtgacgag
     3241 gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac acagagtagt
     3301 aacagggcat cacaaggtag cgatgttgct tcacaaatcg cgcggataag tcagcaaatc
     3361 atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc tgaagacaag
     3421 caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca actggcgcag
     3481 ttgcagaaac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc tctgaatgta
     3541 tcccttctaa atcctgttga aaaaacgact catatcgata tctatatctg atacaacgga
     3601 tccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct
     3661 tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaacc
     3721 accggcgtaa tgcgcttaac caccagtggg ctaccttcag gcaatggtag cgccagtgca
     3781 ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca
     3841 atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt
     3901 cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt
     3961 acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa
     4021 agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg
     4081 acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcatg caggacaaac
     4141 tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa
     4201 atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta
     4261 tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc caactgctgc
     4321 cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga
     4381 ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat acgctctcct
     4441 gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa
     4501 taattcatct gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga
     4561 agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga
     4621 tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg
     4681 cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc tgttctttat
     4741 gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca
     4801 tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg
     4861 gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct
     4921 cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc
     4981 ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac cattgctggt
     5041 tggtctggtg atgaatgttc acggtgcaag gggcagcctt gagcaggtcg gtaaaattat
     5101 gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg
     5161 ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt
     5221 ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc
     5281 attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt
     5341 ctttatggca cgccgactga gcttcaataa ggcagatgaa attactatcg tcttttgtgg
     5401 ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt
     5461 gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc
     5521 ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag
     5581 cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt
     5641 tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt
     5701 gcgggcaaac cacagcagat actcctccgg caggtcaatt aagcgacgcc ctttgtattt
     5761 gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac
     5821 ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg
     5881 ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc
     5941 ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc
     6001 ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt
     6061 tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc
     6121 ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag
     6181 gcaccttttg cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg
     6241 ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc
     6301 gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccaattttg
     6361 gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tctgccggag
     6421 tatggacata ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat
     6481 ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca
     6541 ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata
     6601 ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga
     6661 cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc
     6721 gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc
     6781 cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct
     6841 gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta aacgcaaccg
     6901 cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt gagttgacct
     6961 taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat
     7021 agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaccc
     7081 cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca
     7141 gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa
     7201 gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc
     7261 gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca
     7321 cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg cgaatagtac
     7381 gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta
     7441 aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc
     7501 aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc
     7561 tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt atctggctga
     7621 aagctgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta atcagttctg
     7681 gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa
     7741 tttccggcgc atccatcaca tgataaagat attcatgatg gcaaagcgtc gttcgcagtt
     7801 ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa
     7861 gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact
     7921 tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg
     7981 tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg
     8041 aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta
     8101 gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat
     8161 aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa
     8221 atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga
     8281 tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga
     8341 gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc
     8401 tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac ctgtggcgaa
     8461 actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg caccggctgc
     8521 gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg gaccggttga
     8581 cgcggctgcg cagacgcata aggcggtagg tactggtgtt gcggcgacgg acgagcagcc
     8641 tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac ctcaccaacg
     8701 ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga cttcattcgt
     8761 tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca gaaaccatgt
     8821 accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg caaatcctgc
     8881 atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt actcactaag
     8941 agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca caaagataga
     9001 tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg cgcaattacc
     9061 cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct gattatggtt
     9121 tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg ctggaagctc
     9181 gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa tattttgttg
     9241 atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc gactttcatg
     9301 agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct ggctgtcatc
     9361 tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat tgccgctccg
     9421 tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac accgccagat
     9481 accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga atggcaaaaa
     9541 tttgtctggt atctgccgcg cgcaattgta ttactaattc tttacttcat ccctggtatt
     9601 gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt agccatccag
     9661 tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg caccgccctg
     9721 cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt tacgatgatc
     9781 ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc gatgtgggtc
     9841 gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta ttttgtaaac
     9901 cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca tgtcattatt
     9961 tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga gcgggtatgc
    10021 tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga tttttgaaga
    10081 taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg gtaacggacg
    10141 cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc gtatcggtgc
    10201 caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg aactggttga
    10261 accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc gcggttacaa
    10321 actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc tgaaagcgtt
    10381 aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa tccaaaaagc
    10441 agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat tcagcaatcc
    10501 ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag ataccgacgg
    10561 tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg gcgtcagccg
    10621 ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg agccaaccga
    10681 ttctccagtt atcgcccagg tgctggcagg tgaagagatt aaacctggcc cgcataaaat
    10741 tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg tcgataaagt
    10801 cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg aagaagaagg
    10861 tattcttgca ggtatctctt ctggagcagc tgttgccgca gcgttgaaac tacaagaaga
    10921 tgaaagcttt accaacaaga atattgtggt tattctacca tcatcgggtg agcgttattt
    10981 aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt aatgccagct
    11041 tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg cttcaaactt
    11101 tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga ctaattattt
    11161 tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt atgatttggt
    11221 tcaattcttc ctttagcggc ataatgttta atgacgtacg aaacgtcagc ggtcaacacc
    11281 cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa ctggcgctaa
    11341 caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac taaacctata
    11401 agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa cggtctgcac
    11461 acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga aattactgtg
    11521 acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac tctgggcctg
    11581 actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa agcggttgaa
    11641 catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta aaaatcagtc
    11701 acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc gctttcggta
    11761 aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt tctgccgacc
    11821 aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca gcccagctgg
    11881 aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc atctttgaag
    11941 ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc ctgattaaag
    12001 ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag gcttctgccc
    12061 tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt gatatcggta
    12121 agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc attcaggatg
    12181 aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg aacctgaaga
    12241 aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct atcatggcgc
    12301 gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag gtgaaaaatg
    12361 acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca accaacgaag
    12421 ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca gagcttgcta
    12481 aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta tgcgctaaca
    12541 ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc gttggtctgt
    12601 atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa gaacagtttg
    12661 ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt cgtaccatgg
    12721 acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag aacccgttcc
    12781 tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc gatcagctcc
    12841 gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg atgatcatct
    12901 ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag gaactgcgcg
    12961 acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa acaccggctg
    13021 ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc ggcaccaatg
    13081 atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca cacctttacc
    13141 agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct tctcatgctg
    13201 aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct acacttctgt
    13261 tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg cgcattaaga
    13321 agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag gctcttgctc
    13381 aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa aaaacaatct
    13441 gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg tttgttcgat
    13501 aaactgaaat ctctggtttc cgacgacaaa aaggataccg gaactattga gatcattgct
    13561 ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt tgcggaaaaa
    13621 atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc gcctgtagac
    13681 ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc tgatagcggc
    13741 gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga aggcttcaag
    13801 cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga atttgatctg
    13861 ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc caacatggac
    13921 gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac cccggttatc
    13981 cgcatcaaga agtaattctt gccgcagtga aaaatggcgc ccatcggcgc cattttttat
    14041 gcttccgcca gcggcggcaa aatcaattca tcgctctcat gctgctgggt gtagcgcatc
    14101 acttccagta cgcgcaaccc cgcacggtgc accgcatcgt ttaacgcctt ccctttcagc
    14161 aaaccactga taagctgagc acaaaacagg tcgccagtcc ctttcaggtc ggtttttacc
    14221 cgtgaatggg aaatgacatt cacgctgtcg gcactgacca ccaccacctg catctcctga
    14281 ttttcttcat taccggaggc gctggtaatc accacccatt ttaatgtgtc tgaaagcaga
    14341 ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat ttttaccggt caggatttcc
    14401 agctcaaaga tattgggggt aattccctgc gccagcggca gtaaatattg tcgatacgcc
    14461 tcgggaaggt caggtttgac ataaattccg ctatcaatat cgccaatcac cggatcgacc
    14521 atgatcaata ggtcaggatg gtctttgcgt agcgcagtca gccactcggc aaggattttg
    14581 atttgcgatg ccgttcccat atagcccgtg gttacagcac gaagttggcg cagcgcatca
    14641 cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt ccggaatcgc accaccgtag
    14701 aaagtgtcat aatgcggcgt attgctcagc aataccgtcg gcacggcaaa gacattcaag
    14761 ccgttctgtt ttatagcagg caccgcaatg ctgttgccta cgcttccgta cactacctgc
    14821 gactgcacgg cgacgatatc cgcctgcagc gccctactct tatcgttaaa caacaacaaa
    14881 ctactcattt aattttttct ccttgccgat gatcctcatc gtattccaac cgaaacttta
    14941 cctgattctg gcagtcaaat cggctattac aaaacaaaga taaggtaatt cagtgaagaa
    15001 aatcatttgt ctggccatta cgctgctaat gacactcccc gcttacgcga agttaactgt
    15061 tcatgaagaa gcccgcatca acgccatgct ggagggatta gcacagaaaa aggatttgat
    15121 atttgtgcgc aacggtgatg aacatacctg cgatgaagcg gtttcccacc tgcgtctgaa
    15181 gctcggcaat acccgtaacc gcattgacac tgccgagcag tttattgata aggtagcttc
    15241 gtcgtcatcg attaccggga agccgtatat cgtgaagatc cccggtaaga gcgatgagaa
    15301 cgcacagcct tttttacatg cgttaattgc gcagacggat aaaacggtgc ctgcggaagg
    15361 aaattaatcc gctttggtaa ggcatttaca ggaggtaaca tgaaaaaatg ctttatttat
    15421 cacgatgaaa aatcgaataa attttggtgg atagattacg aaggggatag tttagctgtc
    15481 aactatggca aggtaggtag tattggtaaa ttccatacaa aagaattcga taatgaagaa
    15541 cagtgtctga aagaagccag taaattgatt gccgcaaaaa tgaaaaaagg atatcaagaa
    15601 actccagact ttaacttcat ggatctctac tattttgatg atgaagagat tgggttacat
    15661 cttaaaacgt cacacccaaa cttccagtgc cattttactg atccacttta tatgtgttgc
    15721 tgggatgaag aatctccttt tggtagcgat gaaggtgctg atgctctaaa cgaacttgaa
    15781 aatagcctcc gttctggact gtgctgattt ccctcaaatg ttaattgaaa ctatgtgggg
    15841 tatgaaatac atcgctatgg acagtattct tgaagaggat gtgcgtgcgc aattactagc
    15901 cgatgaaatg agctctatcc agagcaatat gattacctac gcgactgcat tcggtcagat
    15961 caaagtcatg ggtaaaatct cccataaact taaaaagatg ggactcaatg cactagcgcg
    16021 tcatcagctt accgcaaaaa ttcttcaatg gggtgacggt caggactcac caatacttca
    16081 aaaaatgatt gatgacctta ctgcttttcc acacgaaaat taaaaactgc atttgtcggc
    16141 agcaacaact gccgacaaat cagattattg acaaagtgca ctttgtttat gccggatgcg
    16201 gcgtaaacgc ctgatccggc ataaaaatat tgctaattca atatattgca gaaacattgt
    16261 aggcctgata agcggagcgc atcaggcagt tttgcgtttg tcatcagtct ccaatgctat
    16321 taatccttaa atccctgccc cctgactaaa atgctcttcc ccaaacactc cggtggagag
    16381 gtagcgatcg ccacgatcgc agatgatcgc taccaccacc gcaccagggt tagcttttgc
    16441 cacccgcagt gctccagcaa ccgcgccgcc tgagctgacg ccacagaata ttccttcccg
    16501 caccgccagt tcgcgcatgg tgttttccgc atcgcgctga tgaatatcca gcacctcatc
    16561 caccagcgca gcgttgaaaa tccccggcag atattccgca ggccagcggc gaatgcccgg
    16621 aatactgctg ccttcttccg gttgcaggcc aacaatggtc accggtttgg attgttcgcg
    16681 cataaagcat gaaacgccgg tgatagtgcc ggtcgtcccc atgctggaga caaaatgagt
    16741 gatgcgcccg ccggtttgct gccagatttc cggcccggtg gtggtgtaat gcgcataagg
    16801 gttatcggga ttattgaact gatcgagcag ctttccttcg ccacgattcg ccatctccag
    16861 cgccagatcg cgcgcacctt ccatgccctg ctctttggtg acaagaatca gttccgcacc
    16921 ataagcacgc atcgccgcac ggcgttcctg gctcatgttg tcgggcatca gcaatttcat
    16981 gcgatagcct ttcagcgcgg caatcattgc cagcgcaatg ccggtgttac cactggtggc
    17041 ttcgattaag acatcacccg gtttaatttc cccgcgcttt tccgcctcga cgatcatcga
    17101 aagtgccgca cgatctttca ccgaacctgc cgggttattg ccttccagtt ttaaccacac
    17161 ttcactgccg ttatccggcc ccattcgctg caacttcacc agaggcgtat tgcctattgt
    17221 ttgttctaat gtactcacaa tctctatcca tacgtggtgt tgcctgatgc gacgcttgcg
    17281 cgtcttatca ggcctacagg ttacaaacct tgccattaaa aagcccggat cgcgggaagc
    17341 gcctccgggc gtttaacatt cactcaacct atcaggcgct ttgtgcgaga gcaagttcct
    17401 catcgcgggt ttcaatacgc tcgtcgccgt tatacagccg cgcatgttgc agaccaacga
    17461 ataaacgctc gccatgctgc ggggcatcgt cgccatgcat cacgaccgtc agcggttcgt
    17521 tgtaccaccc cagcggctgc accactaatt gggtgtagtg acctttcggg ctggcttcca
    17581 gtacctgtac cggcagcggc gaatcgagac tggtacggcg gctgatatcc acttcccacg
    17641 ggcgcaggaa gagatccacc ggcccctgat acgcaggtgt gtagcccagc ggccagcgat
    17701 gcgcgccaac atggaactgc ccgccgcgaa tggttccctg caggcggttc acttcgccca
    17761 taaattcgag tacaaaacgg gtcgctggtt cgcgccatac ctgattcggc gcatcagcct
    17821 gttcaatatt cccctgactc atcaccacta cacgatcggc tacttcggtc gcctcttcct
    17881 ggtcgtgggt cacaaacacg ctggtgaatt tcagctcttc atgcagttga cgcagccagc
    17941 gacgcagctc tttacgcacc tgcgcatcca gcgcgccgaa cggttcatca agcagcagaa
    18001 tttgcggttc tacagccagc gcgcgcgcca gcgccacacg ctgtttctgg ccgccggaaa
    18061 gctgcgccgg ataacgatcc gccagatgcg caagctggac catttccagc aattttgtca
    18121 ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg cggcagcacc gtcaggccaa
    18181 aagtgatatt gtcgaatacc gtcatatggc ggaacagcgc gtaatgctgg aacacgaaac
    18241 cgactttacg atcgcgcgcg tgcaggcgac tcacgtcggt gccgtggaag cgaatatgcc
    18301 cgctggtttg atgctccagc ccggcgataa tccgcagcag cgtggttttc ccggaaccgg
    18361 acggccccag caacgcgacc atctgaccag aaggaatatc cagtgagata tcgttcagca
    18421 cctgggtgcg accaaaagac ttcttgatat tggcaatctc aatgctcatg atgctcctcc
    18481 tgctgcgcgc gtttttcctg attctccagg cgccactgca acatactctt taaaaacagg
    18541 gtgataatcg ccatcaacgt caacagcgcg gcggcggtaa aggagccgac ggtgttgtag
    18601 tcctgctcca acaattcaat ctgtaacggc agcgaaagtg tttcgccgcg aatcgagccg
    18661 gaaaccaccg acactgcacc aaactcgcca atcgcgcggg cgttggtcag caccacgccg
    18721 taaagcagcg cccagcgaat attcggcaat gtgacgcgac ggaacatctg ccagccggac
    18781 gcgccaagca aaatcgccgc ttcgtcttcc tggctgccct ggcttaacat caccggcacc
    18841 agctcgcgca ccacaaacgg acacgtcaca aagatggtga ccagcaccat tccgggccag
    18901 gagaacataa tttgcagatt atgttcatcc agccagccgc cgagcgggcc gttggagccg
    18961 tagaacagca gatacaccag accggcaacc accggcgata cagcaaacgg aatgtccagt
    19021 agcgtcagca gtaactggcg tcccgggaag ttaaagcgcg tcaccagcca ggccagcaga
    19081 atgccgaaca ccaggtttac cggtacggca atcagcgcga tcatcaccgt cagccagatg
    19141 gcgtgcagca tgtccggatc ggccagattc tgtaaaaccg gcatcagccc cttgctgaat
    19201 gcctgcacga agatgtaaat catcggcacc agcaggatga acgccgaaac cagcatcccg
    19261 atgccaatca gaaaccattt gccccagtta atcgggcgcg cgtcataacg cttcaattgg
    19321 gtaacttccg ccattaatga cctaccacac gccgaccaaa gcgactttgc agagtgttaa
    19381 ttgagaacag cagcagcaga gatgccgcga ggatcaccga agcaatcgcg ctcgctgccg
    19441 ggtaatcaaa ctcctgtaag cgcacaaaaa tcatcagcga cgtcacttcc gtcttccacg
    19501 cgatatttcc ggcgataaaa atcaccgcgc caaattcacc aagactacgg gtaaacgaca
    19561 gcgccacgcc cgccaccagc gccggagaaa gctccggcag caccactttg cagaaactct
    19621 gccagcgcgt tgcaccaagc gtttccgccg cttcttcata ttccgggcct aactcttcca
    19681 gcaccggctg cacggtacgc accacaaacg gaatgctggt aaaggccatc gccaccgcaa
    19741 taccaagcca ggtataggtg actttgatat caaacttcgc cagccattca ccgtaaaaac
    19801 cgtttacgga aaagagcgag gccagcgtta aaccggcgac tgccgttggc agcgcaaagg
    19861 gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa gcgataacgt gttaaaatcc
    19921 acgccatcag cagaccgaaa acgccgttaa aaatcgatgc cacaaacgcc gacagcagcg
    19981 tcactttgta ggccgcgacc acctgcgggt tggtgatcac ctcccagtac tgcgcccagc
    20041 tcatctcaga aagctgcatc accagcgcag agagcggcag cagcaatatc aggcacacaa
    20101 acagcagact ggtgccaagg cttaaagtaa agcccggcag cacgcgtctt gaagaaactg
    20161 caaacattac ttacgccccg ccgctaacag cttgtctaac tcgccgccgc tggtgaagtg
    20221 ggttttcatc acttccggcc atgagccaaa tttgtcttcc acgcggaaca gttcggtctg
    20281 cgggaattta tctttcagtt tgtccatgac ttccgggtta ttcacacggt agtaatagtc
    20341 ggtgatgatg gtctgcgcct gtgggctgta gagccagttc aggtaggctt ttgcggcttt
    20401 ttccgtaccg ttggcctgca catttttatc gacccacgcc accgggaatt ccgccagaat
    20461 attggttttc gggatcacca cttcaaagcc ctgtgcttca tactgtttac ggatgttgtt
    20521 cacttccgat tcaaagctaa tcagcacatc gcccaagcca cgctcggcaa aagtggtggt
    20581 tgcgccacga ccgccggtat cgaacacttc aacgtttttc aggaactggg tcataaactg
    20641 ttcggttttg gctttatcgc caccgtcagc tttatccgct gcgccccatg ccgccagata
    20701 ggtataacgc gcgttacccg acgttttcgg gttcgggaaa atcagcttca cgtcggagcg
    20761 caccaggtcg ttccagtcgt ggatattctt cgggttgccc ttacgcacca ggaagcccat
    20821 ggtggagtag aacggcgagc tattattcgg caggcgcgac tgccagtcag ccgggatcag
    20881 cttgcctttg tcgtgcagga tttgtacgtc ggtcacctgg ttataagtga caacgtctgc
    20941 ttttaagccc tgcaaaatcg ccagcgcctg ttttgatgac ccggcatgag attgttttat
    21001 cgtcagtttg tcgccgccgt tatcttttgc ccattgctgt tcaaacggcg gattcagggc
    21061 ggcaaacagc tcgcgggaga cgtcataaga actgttcagc agttccgttg cctgtacatg
    21121 gcccgccagc agcagagaag cgaccagcgc gagtgagttc tttttcagta agttaacggc
    21181 cattgcgcac ccttataaat ttaatgactt tctaatagcc atcatattta taacggacgt
    21241 gaaaggagta acggttttat ataccgtttg gtgatttgga agttaaaaag ggaataagaa
    21301 cttggttcaa caggcgtaat tgttgcaggc agtttggaca cggacagcgc ggagaaaccg
    21361 gagcgtacac atagtacgtg aggatttcga gcactgcccg gggccaaaat gacaaatgaa
    21421 atagcctggt gaacttagtt caagacgaaa tcctccccac acatgcaggg aggaaacaga
    21481 ggtgaatcag ataccgacgc taaccgtctc cggcagtgtg ctgccgccat caatcacatt
    21541 ctgtgtaccg gttaaatagc tggattcatc cgatgcgagg aaggccgcca gttcgccgac
    21601 ttccagcgga tcggcgaggc gacacagcgg gattgctttt gccatttcag tcagcaccga
    21661 ttctggatct tccgggttcg actggcgggc aatgctttcc gccattggcg tgcggacgta
    21721 tcccgggcag atggcgttaa cgcgaatacc ggattgcgcg tactccaccg ccagcgattt
    21781 agtcaggcca acaatcgccg ctttcgttaa ggcatacgcc gtttcgccag gatcggccac
    21841 catatcacca gtgactgaag acatcatcac aatgcgacca tctttgcgcg caatcatctc
    21901 cggcagcacc gccttcgtga cgttccatac gcctttaata ttgatatcaa tatggaaatc
    21961 gcgatcttca tcgctcatat cgaggaaact gcccagacga caaacgcctg cgttattcac
    22021 caggatatca atgcgccctt ctttttcctt cgcgcgtttg atagctgcgg ctaccgacgc
    22081 cgggtcacgc acatcggcga caaccgccgt acagcgatga ccacgaccac acagttcgtc
    22141 cgctagcttt tcgatctcag gggagatatc cagcaagatt aggttcgcgc catgacgtgc
    22201 aaaagttctg gcaattcctt cgccaattcc ctgcaatgcg cccgtaatca gtgctgtctt
    22261 gcccgtgagt ttacccattt taatgctctc cttgttaata cagagcgtta cactccattc
    22321 cctctgaaaa tacaggataa acaaaatatt gctgcggatc agctcactaa ttcaggaaaa
    22381 tctcaatcca gtcgctgctt tatgcatcat ttcaggcgtt gtgttagctc actgctgcgc
    22441 tgaatcattt tcaatgacgc cacatcattg atgttgcaca agaccaacaa acagtaaatc
    22501 ggtaacagaa ttttgcgccg tgcgggtaga catcgacgag ctacgccatt cggtttcacc
    22561 ggaaacggta tcgagcgtga aatgcgccag ccgccgcagc ggagagtcag ccagagaagt
    22621 aatggcaatc accgttgccc cctgctttcg tgccgcttct gcgcacaaga caatctcttt
    22681 tttactgccg ctgtaagaga tagcgatttg tacatcgcct tttttcaacg cctgggaaac
    22741 ggtggcttgt acgtgcgtgt cggcttcgca ggccacgcga tagccaattt tcatcagttt
    22801 gaacgacaga tcgcgcccca ccagcgccga tccgcccagg cccgttatct ggataaacgg
    22861 tgctttactg atgacctcaa tgattttttg cagccgcgcg tagtcgaaca acgcacaggt
    22921 ttgctccagc gccagctctt tttcgcgatt cagtttgcgg gcaatcactt ccagcgaatc
    22981 atcactggta atcgaactgt gcaggtgcag cgccgtggca ttcgttttct cccggctggc
    23041 actgtattcg ccaattaacg ccatacgcaa ttcagtaaat ccttgcgcgc cgagcttctg
    23101 ggcaaatttc acaatgctcg actggctgat accgagctgt ttcgccatct gccgtgaaga
    23161 gacggatttc agctcgctca ctctggcccg cagaaagtca gcaatttttt gctcgttccc
    23221 ggtaaattca ctttccgcat tgcgtatttt cgtcaggtac aacatcttcc actctcctgg
    23281 gctggcagca aaaattccgc ttaagttctt atgacgctct tcacactctg cgagtgtaga
    23341 ctcaatgatt cctttaagtc tgtcaattcg ggatatatga ttccatacat ctgcaattat
    23401 ttgatcgtaa atagtaaggt caccaccgat gcaactagaa aagatgatta ctgaaggctc
    23461 gaacaccgcc tcggctgaaa ttgaccgcgt atcgacgctg gaaatgtgcc ggattatcaa
    23521 cgatgaagat aaaaccgtac cgcctgccgt tgatcgcgtc ctgccggata tcgccgcggc
    23581 gatcgatgtt atccacgccc aggtcagcgg cggcggtcgt ctgatttatc tcggtgcggg
    23641 aacatccggt cgtctgggga ttctggatgc cagcgaatgt ccgcccacct acggcgtgaa
    23701 accgggtctg gtggttggtt tgattgctgg aggcgaatat gccattcagc acgcggtgga
    23761 aggagctgaa gatagccggg aaggtggtgt taacgatctg aaaaatatta atttaacggc
    23821 acaggatgtg gtggttggca ttgctgccag cggtcgcacg ccgtatgtga ttgccggact
    23881 ggaatacgca cgccagctcg gctgccgcac agtgggaatt tcctgtaatc cggggagcgc
    23941 cgtttcaacc accgctgagt ttgccattac accgattgta ggtgccgaag ttgttaccgg
    24001 ttcttcgcgg atgaaagcag gtacagcgca gaaactggtg ctcaatatgc tttccaccgg
    24061 gctgatgatt aaatccggca aagtgttcgg caacctgatg gtcgatgtgg tcgccaccaa
    24121 cgaaaaactg catgtgcgcc aggtcaatat cgttaaaaac gccaccggat gtaatgccga
    24181 acaagcggaa gcggcgttaa ttgcctgcga gcgcaactgt aaaacggcca ttgtgatggt
    24241 gctgaaaaat ctcgatgccg cagaagctaa aaaacgcctg gatcaacacg gcggctttat
    24301 tcgtcaggtt ttagacaagg aataacccat ggccaaagag atcagcagtg aacttctgaa
    24361 caccattctt acccgtgtcg gcggaccggg aaatatcgcc agttgtggta actgtatgac
    24421 gcgcctgcgt ctgggtgtac atgacagttc actggttgat cccaatatca aaacgctgga
    24481 aggcgtgaag ggcgtcattt tgaccagcga ccaggtgcag gtcgtttttg ggcctggtaa
    24541 agcgcatcgt gccgcaaaag cgatgagcga gctgctgggc gatgcaccgg tacaggatgc
    24601 cgcagaaatc gccgcccaga acaaacgtca gttaaaagcc aaacaaacct ccggcgtgca
    24661 acaatttctc gccaaattcg ccaccatctt cacgccgctg atccccggtt ttattgccgc
    24721 cggtctgctg ctggggatag cgacgttaat tgccacggtg atgcacgttc cggcagatgc
    24781 tcagggcacg ctacccgacg cgctgaattt tatgaaggtg ttcagcaaag gtttgttcac
    24841 cttcctagtg attctggtgg gctataacgc cgcccaggca tttggcggca cgggcgtcaa
    24901 tggcgcaatt attgccgcac tctttttgct cggttataac cccgctgcaa ccaccggtta
    24961 ctacgccggt tttcacgatt tctttggtct gcccatcgat ccgcgcggca atattatcgg
    25021 cgtgctgatt gccgcgtggg cctgcgcccg cattgaaggc atggtgcgcc gctttatgcc
    25081 ggacgatctc gacatgctgc tgacctcgtt aatcaccctg ctgatcaccg ctacgcttgc
    25141 gtacctgatc attatgccgc tgggtggctg gttattcgaa ggtatgtcat ggctgtttat
    25201 gcacctgaac agtaatccgt tcggttgtgc ggttttagcc gggctgttcc tgatcgccgt
    25261 ggtatttggc gtgcatcagg gctttattcc tgtttacctc gcgttaatgg acagccaggg
    25321 attcaacagc ttatttccca tcctttcaat ggcaggcgcg ggccaggtgg gcgcggcgct
    25381 ggcactctac tggcgggcgc aaccgcacag tgcgctacgc agtcaggtac gcggggcgat
    25441 tattcccggc ctgctgggcg ttggtgaacc gctgatttac ggtgtcaccc tgccccgcat
    25501 gaagccgttt gttaccgcct gtttaggcgg cgcggcgggc ggtttgttta tcggattgat
    25561 agcctggtgg ggtctgccga tgggcttaaa cagcgccttt gggccgtctg gtctggtggc
    25621 gctgccgctg atgacttccg cacaaggcat ccttccggca atggcggttt atgctggcgg
    25681 gattctggtg gcatgggttt gcgggtttat tttcaccacg ctctttggct gccgtaacgt
    25741 caatctggac tgatattatg aaacggacaa tgctctatct ttctctgctg gctgttagtt
    25801 gtagcgtcag cgccgcaaaa taccctgttc tgacagaagg tagttgatca agtatatgcg
    25861 gcgttaaagc agtaataaaa aggccagtca gtaatgttac tgcctggcct ttaatcaatg
    25921 aatcagaaac gcttacagcg ccatcaactt gtccagcgac ggtgcgaaat aatagccgcc
    25981 ggttaccggt ttggtgaaac gcaacatcgc atcacgtttg ccatcggtat cgccaaacat
    26041 gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc
    26101 gtgagtgcca ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc
    26161 atcttctttc agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat
    26221 ctcttcgttg gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg
    26281 gttgagctgc ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggaatc
    26341 cacgccgtct ttgataaccg ccacttcgcg gcgcgtctct tcacccgccg ggttttctgt
    26401 accgtcaaca aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc
    26461 tttcacttca atgcagtcac caaaggcttc catcgccgcc tgagcgacag agaagtttac
    26521 gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac tgggtcgtcg gcgcaaggcc
    26581 tttaccgtag cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg
    26641 ccaggtgttg ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc
    26701 aaaagttgcc agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc
    26761 tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg
    26821 aacctgagac attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag
    26881 cgctatttta cgtgtttttt cgcgacggcg gtttgttcag acgcaaatta acgccgccag
    26941 ataattttac tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga
    27001 ccacgccatt ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg
    27061 acggcacgat tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg
    27121 tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg
    27181 tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc
    27241 atcacttcaa aaaagcgcac cacgttgccg ttcgtggttt tcattccgct gcgcaggcga
    27301 taatcgccat caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat
    27361 tcacccacgc cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg
    27421 gcagaccagt taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat
    27481 aacattaaac gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag
    27541 tgcaaattca ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt
    27601 cgattggtca gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata
    27661 ccgctaacgg attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc
    27721 gccaacagca acagaaaata gagcacatgc caggtgcgag gaaacgaagc ccgccgaccg
    27781 ctcaacaaaa acgccagcac cgccggaata ccgggaatca gccccagcca gaaattatcg
    27841 tgatcgggat aaaacagatt tagcagcgca gtaccctgct cacgcgacgc accggcaatg
    27901 acaaacagca cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa
    27961 cgcaggcgtc cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat
    28021 caaacgctta cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc
    28081 gagcaccatg tcgttatctt ccggcacgtt gatctgaatt ttcgggcagc cgcgagcaat
    28141 cagctttttc tccagccggt taagcaacgc attggcaatt ccacgcccac gaaactctgg
    28201 atgcacgcca agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc
    28261 gaccacttca ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg
    28321 ctcgatgtcc atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt
    28381 gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt
    28441 tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa
    28501 atcggttgaa aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa
    28561 ttctcaacag caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat
    28621 tattacaact caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc
    28681 caggtgctga aagccggttt agctgccctg acgttgtcag gaatgtcgca agccattgcc
    28741 aaagacgaac ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc
    28801 ggcaaacgtg tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga
    28861 cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt
    28921 ttgcgtaatc atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt
    28981 tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc
    29041 gatggcttta ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg
    29101 gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc
    29161 ggtaaaaagg cgactgacaa ggatcaccta ttgcagcaag tactgtttga tctggtgcaa
    29221 accgatacta ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg
    29281 gtgcataaac tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg
    29341 tcggttcctt cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg
    29401 ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt
    29461 tatttccact ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc
    29521 gacgcacacc aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg
    29581 accgccgtcg atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc
    29641 gggcgcagtc gggtgttgcg taatggtggt gttttcgaac aggcgggcgt caacttttct
    29701 cacgtccacg gtgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg
    29761 cgcagtttcg aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc
    29821 accagccacg cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtttgg
    29881 tggtttggcg gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac
    29941 tggcaccgca ccgcccgtga cctgtgccag ccatttggtg aagacgttta tccccgttac
    30001 aaaaagtggt gcgacgatta cttctacctc aaacatcgca acgaacagcg cggcattggc
    30061 gggctgttct ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag
    30121 gcggtaggca aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg
    30181 gcctacggcg agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc
    30241 aatctggtct gggatcgcgg cacgctgttt ggcctgcaaa ctggcgggcg caccgagtcg
    30301 atcctgatgt caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc
    30361 agcccagaag cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac
    30421 ccccattccc gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg
    30481 taatccgtgg caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc
    30541 gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta cgacgttcag gcgaatgcgt
    30601 ttcagccacg cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg
    30661 cgactgacat gcagttgatt acacaaatcc agcaccgtca ccggttcgga catgttttcc
    30721 agcacatatt cacgggcgcg ggaaagcaac cgacggtaac tctgatgact gatgctttcc
    30781 gccgtcacca ttggttgcgc ttcttccagc atcgccccca tcgccattag caaattatcc
    30841 cccagcactt ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc
    30901 agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc
    30961 gactggctac gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacag
    31021 gtgatgacat cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat
    31081 tcaaactcgg ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg
    31141 ataaatccct gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc
    31201 aggcacgact gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagt
    31261 agaatttccg taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc
    31321 taggcggtaa tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta
    31381 tccacttcga ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg
    31441 tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg
    31501 gcacctccga caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc
    31561 ccggcagaaa agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg
    31621 gcggccactt ctcctgccgt cattccctga catactgatg ttaacagcgc caacaactcg
    31681 tcagcagatt ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg
    31741 cggttaaagc cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttacgg
    31801 ctgatgacac gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac
    31861 gccgccagat cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg
    31921 agcagacgaa cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg
    31981 ccgtccactt ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg
    32041 attggattac gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac
    32101 gcggcctggc taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggacg
    32161 taggtcacca gctgtacgtc ggcagatttc aacgcggcat caatgccgta ggtcgcttcc
    32221 agcggcggtg ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa
    32281 agataagaac cggtacgcga aaccacatgc gccaggaagg cggtgttttc tgcgtcattg
    32341 gcccactgga aagctgcgcc attttcaata tgtgcaacca tcgcatccag accggcacgc
    32401 acttccgccg ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggg
    32461 ccgtgtgccg ctcccgcata cagcgagcga ccatatacca cttcgaccat cgcttgtttg
    32521 gtcgcttcgt cagccgcaat gtaggttacg tcgtcagaat cggcagaaat cagcccgagg
    32581 ctacgaatat gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc
    32641 acccgcatgg cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat
    32701 cgggtcatgt tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg
    32761 acggcacagc agcacgaaag agaagcaaaa gtcagctacc tggtacacta ggcagcgggt
    32821 cagcaaaggg gggcaaatgc cccttttttc atgtcagata atcagatcat tacctcaata
    32881 aactaataat cacatcgcgg ttacaaccag tttgatagtg atagctcaaa cccagacgtg
    32941 gcgctggctg caagcaaaat aagccgcaaa aatgcgaccc catgtttact agccgcaaaa
    33001 atgcggtcct atttgccaga accttttatt attcaattgg ttagtgcaag aaaaagcgtg
    33061 attactgttc aagaagtatc cagaaaggtc atgattttga cgctgaaaaa aaataggtgg
    33121 gcatttttgc ccactcacta gccgcaaatt tgcccactca ctagccgcat ttttgcggcc
    33181 caaagtaata aatacagaga aataataaat acagatcagc gccaagattt ttcgtctttt
    33241 cggcggaatc atgagactag tgcttacgtc ctgataacct ccagagcaga ccttaacttc
    33301 tgtgcagcag gactacgctc attgccgctc ggctaggtca aatcctcacg ataaagctgt
    33361 tcggattgtg accataatgt taaaggactc tttaaatcat tccttgacga attaactagc
    33421 tggcttaagc ggaatagcat gcaggataaa aacaaaacca gcttagcagt ggggagagct
    33481 tcgcttcgct cgtctcctgg ggccaatgct caatgaaacg acgtaaggca cggagcgctc
    33541 accataggta gacacataaa catctgagag cgcaccttct atcgcgctac gcgctcaata
    33601 aaaaccaacg acaccgccac actgcaagac caatggtttt tatcgcgtat acagaccaga
    33661 caggaaacag cagcaaagca gcgtatagcc aggtgcagac agcacactca cacgacgcac
    33721 agcaggctta agtgatcact caggaaactt tatccccgcc gccgatcacc gcgaactatc
    33781 atgaccgcgc caaattcgaa aggaatggcc catagaacgc gatgaacaca cataagcgct
    33841 acacttactt aacgtcgcca cagagcgatt gagagcgctt aaaatcgatg cgatttcatt
    33901 aaatttttct cactcgcctg aatggtggcg atcaggactg atcgcaacat tgccccgcct
    33961 cgatattgag cgcataatag atacatacat taagcggaaa agagataagt ttattgtgaa
    34021 aatcaggggg ttacatatca tgcgcatatc agaacaacaa cggcagatcc tcgactatct
    34081 ggcaaaaaac gaatcaatca ccgcacgaca agccgcagat ctcgtttatg gtggcaatgt
    34141 tacccgcgtc caggttgacg ccgcccgccg ctcactcatg acgatggtta gtaatgggct
    34201 aatcaggaaa caaggccgcg ctttcgtagc tgaaagacat atcaatcacg cttatagcca
    34261 ggacgttgaa gaattgcgcc gccagatcct ttgggtattc agcaaacagg gtcaggagta
    34321 tttcctttct gaacacccaa atttcgccgc caaagagtta acagtaagcg gattgtcgtg
    34381 cgcactggat tatcttgtcg gtgaaggttg cgttacttac aagcagctat ggcacgccct
    34441 gaatacgctt gtagaagatg gtttactcat tgccacgcgt gagaacatcg aatactggat
    34501 aatgccgcgt tcaggtcgcc gcaatcatgc acggtatatt tcaggcaaca gatacagaac
    34561 gccggacttt aatccgacac aggaagatcg ggaccgtgcc gaaatgatgt gcagggaaca
    34621 ggagaaaaga attatggata agttattttc gatgcagtga acacaagcgg gcgcaatgcc
    34681 cgtttttatt gccttattcc acgcctgacg aatcaatttg atcacacctg agaaaatgca
    34741 tagccagaag ttaaggccgt tatattgcga ttgggagcgt tttaaacggt atcaatttaa
    34801 ttaattctga tgtggtgatt tgcaggcaaa aaaaacccgc cggagaagcg ggaaatagag
    34861 acatatcgtt gtagttatta acagatcact ttgaggtcga gggcggctaa ccctcgctaa
    34921 tattatcccg ctattacctt gcgggtgcaa ataatctttt attgctttga tgatttaata
    34981 aatagcactt tttgctaaca aaaaccgctg tcaatatatg gccaaaaatg tcaacagaaa
    35041 aaataaggtt tataatatta agtatcacat gggtaatggg ttcagggaat cggaatccac
    35101 cgcctcacgg aaattcaaaa tttgcttctt ctttcctccg tgggcggctt ctttttcgta
    35161 tcgaggtatt tatgaaagca ataacattag aacagttcgc agcaatggcg aatgtatcga
    35221 ccagaacgct gaggcgctgg ctaaaaaaac catcaaccgg agtccctttg ccatttaccc
    35281 cacgcggtac tcgccaatta ttctgggagc acgaatgcaa agaatggttg tacagaacag
    35341 aacgataata atcacgcatc acattttgag gtaaacatca tgaccaatat cgaaagatta
    35401 gaacgctgtg ccgatattat gcgacgcaga tggatttatg accctgaagc gggtttattg
    35461 tttagccgtg aaacaaaaga cgttatcaaa ggcagcatga ccaataaagg gcatttggtt
    35521 gtaacggtac atgaaggtaa attcaaagtg acactcacct atcagaaagc ctgctatgta
    35581 tacacatacg gcgcttatga cgagacatta tatgaagtgc accacgttaa tttgaacaaa
    35641 caggataacc gcatcaagaa tattcgcctg atgccgaaag aaaagcaccg ccggatacat
    35701 aaaaacgtcc gcaaacttat tcggctgggc cacacacaat ggcttcaacc gcaattaatt
    35761 gaacaatcac tataaacaca ggagtaaata ccatgcattc attagacagt tattttcagc
    35821 gcacgacagc ccctaaatca gccgcccagg aacgccgtga ggaattccat gagaaagtga
    35881 tgcgttctgc tgattatatt gcggacaagt tcgtggaaac ggttcgtcca cttgttgatg
    35941 aagtagctga taaacttcag tctgaaatgc cggaagatat ggaaggcact gcaaagcggc
    36001 gactcatttg cgaattaagt cgtcgttttg gcgtgtcaat aagcgctttt aaataatcga
    36061 aaaaaggggg gaaagataat ggacgcattt gaggtactgg caaataaagg cgctgaactg
    36121 gtagcacagc gtgataaaac cgccaacgaa ggggaacgcg cactgctaaa taaacagatt
    36181 aaagcgatcc gtatggccca gtttaaatta atcagtaatg aagttatcga aacaattaaa
    36241 ccgcagatcc cacaaatcgt tgctgacgct attaaagccg ccggactgga gaaacgcatt
    36301 gccgggctaa aaacgaacgc agagaaaatg gagatgtaca aacagagcgg tgctccgaat
    36361 cccgatgagt acttctcacc ggatgttgag tttatggcgc aggttgagga acgtctgggt
    36421 agctgtctca ccgaggaaca gcgtcgctat tttgatggag tggatagttc ggcaggtatt
    36481 gacctgaact cgtatttcgg tcgtgaaatt gagcattttg atgccgcccc gtctatgccg
    36541 gagccagaac cagaaatggc caccgatcct gaagctgagc aacggaaacg cgtattcaat
    36601 gcgataggga ttcggtactg atcggataaa agaaagccgg gaattacccc ggctgactta
    36661 acaatgtgac caatgaacaa atcggacaag acaaacagga taagcagtct cgaaacaatg
    36721 ttcgggaact atattagcac aagtccggag aaaatcatca tgttggcact acgcatcatg
    36781 cagggcatcg ctaagaccct cgcagaacat gtcttagacc tgaaacattc gccactgagc
    36841 aaacaggcca tgaagcgcca aacgcttcgt ctgtgggctg aatattcgct tggaactatc
    36901 aataaaatca tcgacatgaa atcggggccg agcaaccaga gcgcggagga aatggagttc
    36961 atcaggcgtt taattctcat tcgcagagac attcatagcc agttacacag cgtgggaatc
    37021 gatattaatg atgggacggg cgattaattt tcggacaagt actatgcgcg cgcgaggagc
    37081 tgaacatgaa gcaagatatt gcagacaggc tggagatact cgaaggccag cgggcagaag
    37141 cgaaacaact acgtaagcag gccagaagag cgcacaggaa caacgaagca gagttactaa
    37201 ccaaatacat cagtttcacc aattactgca tctacgagtg ttacaaggaa gatgcagagg
    37261 actggctgga ttcattgcca gaacagtact gagcagaata acaccacgtc atgttgttct
    37321 gaggtgagca tatcacctct gttcaggtgg ccaaatttag tgtgccactt cagcatcacc
    37381 gttgaaatca caataacgat ttttttgtaa atatgctctc acatatgtag gaatagacca
    37441 ggttggcatt gaaacacatc taccatgacc ataataaagt aattttttga tatgtttatg
    37501 gttttatatt aattaaaata tatctaaatt tgatagtttt aaaattgcaa aatccagatt
    37561 gtgtttgtaa tgtttaatct gattgtattg agtggcgttt attatgaaaa ataaatcagg
    37621 taattcttgt tatagagaag cagtcgatat tcatagctca cttgagcttt catttatatt
    37681 gcataaatat catttgcgtg tagatgagaa gaaatctcga aatgtattca gcgccatacg
    37741 tgatgagctc atccttgatg gggattcacg acaaaactta gctactttct gtcaaacatg
    37801 gcttgatgat gaaatccgtg agttgcttaa tttagctatt gataaaaaca tgatttataa
    37861 agatgaatat ccacaaatgg cggaacctga aggtcgctgt gtgcgtatgc tttccgattc
    37921 gtggagtttc cccgattcgc gacacacctt aggttgctca accattggtt catctgaagc
    37981 tgctatgctc ggccgcttgg cgttaaaatg gcagtggtgt aaaaaacgcg aagtacaagg
    38041 taaatcaacc gaacctgatt tgcgatcctg tgcaaatatg ctggcataaa ttctgccgtt
    38101 actgtgatgt gaaattgtgt gaaattccac ttgatgatca tcacctgata atgaatgcta
    38161 aagaggtttt gaagcatgtc gatgaaaaca ctattggtgt cgttacgact ttaggtatta
    38221 cttatttttg taaagcagat tgcgttagtg tttttaagat aagtgttaaa gttaatatta
    38281 gtttttatat ttacatcgta attatatgtt tctattttaa aaatgttcga gagtataaaa
    38341 atgtctaatg ctaatatcgc acccaaaagt aagaaaatga cagtggggac actcgctatc
    38401 atgaatataa ctgctgtagt aagtctacga tttatacctt ctgaagcaga atacggctta
    38461 ggtgccattt tttactatac ctttgctgca attatgtttt tagtacctgt tgcctttgtt
    38521 gcggctgagc tagctacaac atatccacaa aaaggggggg catttcgatg gataggagaa
    38581 gcatttgggg ctcgctgggg ttttgtagga atgcttatga cctggctaca agtcatcccc
    38641 tactttccaa cagtactgac atttggtgca gtctcggttg catttattga tgttgatatg
    38701 ggggtggcag aatcaattgc cagtaataaa tggtatattt tatttttcgt actatttgtt
    38761 tactggggag ctatttgtgt tgcattacgt ggcgttggta ttttctcgca ggtatctaag
    38821 tggtgcggta ttatagggac aattattcca gctattatcc ttgttattct tggtttctcc
    38881 tatctgtggt tcagcggtaa accagcggtg atcccattag gttggggtga tcttttccct
    38941 aatatagcta acttccagaa tattgttctt gccgccagca tatttcttgc ttatgcaggg
    39001 atggaaatga atgcggtcca tgttaacgat ttagataatc caactaaaaa atacccaatt
    39061 gcaattacca tcgcttctat aggtactatt gcaatattcc tgctcagtac tttaggggtc
    39121 gcctttatta ttcctacaga taaaattagt ctaactcaaa gtttgttggt tgcctacgat
    39181 atgctgttcg aatgggctgg agtgccatgg cttggctctg tgatggcatt tatgctagca
    39241 attggcgtac tgggtggggt ggttacttgg attgcagggc ctaatacagg tgtattagcg
    39301 atcgcgaaag ctggttattt acctaagttt ttccaaaaaa ccaatcgtca tggaatgggg
    39361 caccatctaa tgtttgtaca aggtataatt gtctcagttc tttctgttac ttttgtgata
    39421 atgccttcag ttcaagcagc ttttcagatt ttatcacagt taacagttat gctctattta
    39481 gttatgtata tgctcatgtt cgctagtgct atctatctac gacattctca acctgataca
    39541 aaacgcccat atcgagcccc tgctttattt ttctgggcaa cacttggatt cttagggtct
    39601 tttctggctt ttgtattaag ttttattccg cccgagcaaa tcccaactgg tagtccaacc
    39661 agctatgttc tttatttgtt agtattagtt gccctatttt tattaattcc aatattaatt
    39721 tacgccttcc gaaaatctga ctggaaagat ccaaactctg atttcgaacc attttcttgg
    39781 gaaaaaagaa ctagtaatta aattttccat aagctacaaa agatgaggtt aaatatgttt
    39841 agtgctgaat ctccaattgg tgtaatagac tcaggtgtcg gtggattgac atcggtaaag
    39901 gaaataatta atttgcttcc tggtgaagat attatctatt gtggagataa tatgaatgca
    39961 ccttatggca accgttctgc cgatgatatc atcagtttga ctaaaaaaat gctgactttt
    40021 ttacagtcaa ggaatgtcaa actagttgct gtcgcatgta atactatatc ctcaactttg
    40081 gaatccgaag aatacagcgg atatgctaag agctttccgt ttcctatctt atccatcatt
    40141 gaacctgcag tagaagatgt gattcgccag caatataaaa atgttggtat tatcgctacc
    40201 gagtttacta ttaaaacggg gtgtcacaaa gagctaataa aaaaattgaa ttcaacaata
    40261 aatgtatttg gagaaccaag caaaaatctt gcaatgttaa ttgaggaagg taatcttaat
    40321 gctcctgcaa tcttgaatga catcaaaaaa catgttaacc atttaatttc cctccatcca
    40381 gtaaatgaaa ttattctcgg ctgtactcac tatccaattg ttcagaattt ttttgaagca
    40441 gttgcaccgg atataaaatt tattaatcct gcacatgatc aggctatctc tattaaaaat
    40501 cacttgggac agctaaattt attaaactcc tcaaatatag gaactttgca aataaatact
    40561 agtggaagta tggagatcta caaaacggta ctaggggaat tgagcattac gaaacctcat
    40621 acattcagta tcaggcagtt ttaaatttat ccggtacacc gtaaaatccc gtccttctga
    40681 actgcacccc aaaagttgga caccaactga gtaaggtgca ttttatggac aaacccagtt
    40741 acatactggg tagtgcatcc aattagtaga acatgtgttt ttcgataaac gctccgatca
    40801 ctttttcgtg gatctcaact gagcgtgaga agcagattgt tttacgaccc aaccgcttaa
    40861 tgcgggtgcg tagcgtcaga ttattacgct caatgcgttg ggtgaatatt ttgccggtca
    40921 gatgcttatt cttcggcacc tcccggccat agctgcccca gtcatcgctg gtcagcatgc
    40981 cgatgttgaa gggtgtaagc agtgccagta gctcccggca cgtttgatcg gttcggggac
    41041 caaaagtgta ggccagtaca ccgcctgttt tggtgttgta cgcgtacccg agccaatgtt
    41101 gccgggcttt actgccaacg tagctccatt gctcatcaag ctcgcagata aacgccacat
    41161 cagcatgggc aacaggcgaa gaagttattc gctttggcgc gagtttttta aagtccggat
    41221 gacggtgtta ctaccaattt tcagtgtcct ggcggtatcg cgaaccccgg caccattaaa
    41281 ggccatttca gttatcagct ctttcatacc cggcttacgt gcttgataag tataagtgaa
    41341 ctgaaacaca cggtggcagt cacggcagcg aagtctgtca cggcctttag ggttctgacc
    41401 atggcggtaa acctgagctg actgacaacg gggacaatga atattaacgc tggtcatgag
    41461 ataacctcaa aagcccgtat tatacatcag attcaactaa ttagaggcat cacccatcca
    41521 gcattataaa acatactaac aataactaaa ttataataat cacaacttac aatcaaataa
    41581 taaatgcata agattaacaa tgattttttc gggttatatt tatctaaaaa agcagaacat
    41641 tatgtaactt attattttct aagcgaaaat ttattgtgcg gtcagactaa caaacttgcg
    41701 taccacccga agaacatgcg aagatttaag tattctttct tttttggttt ttatttttca
    41761 ttttctcttt tcttctcttt ccggttctgt tctggttttg gttttttcct ttttattttt
    41821 attctttctt cagggttatg ctaaaacacc aaaacatttt tcaccgcaaa agcatccaat
    41881 catcttgcca gaccaatcac cttaaccgga tacactgccg gatataatcc cactaacacc
    41941 cagacaccaa gcaccatacc tgataactac cgtattcagg aaggaaatgc gagtgatttc
    42001 accgtcaccg gcacaaccga tccgccaaaa agaggcgtac caatgtcgat atagtccccc
    42061 gcgcggacaa tcacttcgtc aatgactgcc aacgggagtt gttgtagctg tgggcgcaac
    42121 aacatgccca gagctttacc aaagtcctgc ccggccacca ccagcagggg atgcggattc
    42181 ggaaaacgcg cgacgaaatc gaccagcgcg ttgatgaccg tcagaaccgc ggcgtaacgc
    42241 acaggcagcg aggcgggaag cgccagcacg tacacgtcag ttttgggatc aagatccagc
    42301 tgaagcagcg cctgttgcca ggcactcacc agatccgttt catcaatcgg gatcgccacc
    42361 ggcaaattgc gcagcggcag ttgtacgccc tccagccaga ttgtgctgcc agagagcgaa
    42421 agggtatgtg cacccgcgcc aatcaccgtg gagcgtacgg tttgcgccgg aaactgcaca
    42481 ttcatctcac gcaggcgcgg atggtcatgc agcgccgttg ccagaagtgg gccaatatcg
    42541 gcaaaacaga acgggttggc gggctggtgg cgataacatt cgcccacgcc tccagaaagc
    42601 gtaatgattt cgggcgtaac acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg
    42661 agcggcgaaa gcgttccgtc aatcacttcg acaatcagcg ccgccatccg ccgggtaacc
    42721 tgcaccagct gcgcgccggt cagcgaacgg gcgtcagtgc ctgcaccgaa gcactcatcc
    42781 acaatcatct gccccggttt atgagcgtaa accacgcgcc cctggctgtc ggtttccagc
    42841 aggcgaccac cgacgttgag acaggcagtg ccgctgattt ttccggcatc gaacagggcg
    42901 tagttcgcgg tgccaccgcc gatatcgata ttcagtaccc gacacagccg ttgttcagaa
    42961 agggtttgcg ccccggctcc gtgaccggcg atcacggatt cgaggtgcgg cccggcgctg
    43021 gcgacgacaa aatcgcccag cgactgtttt tccttcatca ctttttccat cagctgtgcg
    43081 accaggcttt cggtaaactg gccttccggc agctgggcaa tgatggtttc gcggatgcgc
    43141 tggctttcgg ttttgtcggc tggcacgctg gcgggcacgg acgccgttac cgggatcgat
    43201 tcatcacatt cggtgatggt aaaacccagt agatccgcca cttcacgggc ttccggggta
    43261 ataatgctgg cgcgcagaac cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga
    43321 atatcattcg ctgtgatgag ttttttcacc tgcttcctcc tgtttggtga gagatgccag
    43381 ataatccacc agttgctgca cgctttgcgg gtcgtggctg tttagctcga aaatcggctc
    43441 ttcgaaaccc gtctcaagca ataactttcg cgttgcggcg acgtcagcat ctggcatgtc
    43501 cgttttactg atgacggcga tttgccgttt gctgacgcca atatccagca acccggcagg
    43561 caaccgactt tccgggtcat ttgcgccatg gacatagatc agcatgtcta catcctgtag
    43621 cgtggtaatt aaggcgtgat accagcgggg atggctaaaa tattcacccg gcgtatcaat
    43681 atcgccctta tcattaaatt ccacggccag tgtttttctg gcgagggtat aattcccctg
    43741 caacgcatta aataacgttg tttttcctgc cccgacgcta cccacaaacg caattcgttt
    43801 catgaatgcc tcgtattaac ttttggtcat ttcacaaagc gtataattta ataagcgacc
    43861 taaaccgctg acggtttgtg ataacgcttc ttctactgcg cccaccgagc cataaatcac
    43921 caatgcaccg ctgaagcggt cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa
    43981 atcaccggcg atcatcgccg tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc
    44041 atccggaacg ccgattttct tcgccagttc ctcaccggga tgcgcgatga gatgcgccag
    44101 tgtgacctgt ttgccgggca caaattcctg aatgatgcgt tctttatcca tgagtcgccg
    44161 cctgtgaaaa actttcgtgt atcgtgacaa tttttcgtca gagtaaataa caaaattcgg
    44221 caagcgcgtt taaaaggtga ggtagatcaa taagaatgga gagatattta ctgttatgaa
    44281 taaactttca tatgcgacgc atgaatataa ataaaataat atcgagatat tttatatact
    44341 gattttttta ttattcgcaa actcagtaat aagctttgct aatatatttc aggaaaaccg
    44401 cttaccctga atattcaggg taagcgtgag agttaagaat gaattacagc ggttgggttt
    44461 gcgcttcaac cacggccagc gccaccatat tgacgatacg acgcaccgat gcgatcggcg
    44521 ttaacacgtg aaccggtttc gccacgccca tcagcaccgg accgacagtc acaccttccg
    44581 agctggaaac gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca
    44641 gaatattggc agaacctttc aaagggctgt ccggcatacg gtcgttgcga atcgcttcca
    44701 ccagcgctgc atcgccgtgc atttcaccat caatcatcag atctggcgca cgatccctga
    44761 ccagttccag cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg
    44821 agtgcgacaa cagagcaacg cgcggctcaa taccaaaacg acggacagtt tctgccgcca
    44881 tcaaggtgat ctccgccagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa
    44941 taaaggtgtt accactcggc agcagcagcg cattcattgc acctgcggtg tgaacgccat
    45001 cgcgataacc aaagacattt ttcaccacac taaaatgttc atgataatcg cccaccgtac
    45061 cgcagatcat tgcatcggct tccccacgct gaaccatgat cgcgccgatc actgtcgggt
    45121 tactgatcag cgcacgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga
    45181 agtattcggt ccagtactct ttaaagcgcg gatcggattc gttattgacg atttcaaaat
    45241 caacgcccgc tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac
    45301 gaccgataag gatcggtttc gccagtccca gcgttaccag ttcctgagtc gcatgcagaa
    45361 cgcgcgcctc ttccccttcc ggcagaacta cgcgcttcgg cgctttgcga gcctgggaga
    45421 aaatcggctt cataaacagg ttggttttgt aaacgaactc agtcagcttg tcgatgtaga
    45481 cgtcgaaatc agcaatcgga cgcgtcgcca cgcccgactc catcgccgct tttgcgaccg
    45541 caggagcgat cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac
    45601 caaagctcag atcctgaacg ccatacgctg aagccaccac ttcactctgt tctgcatggg
    45661 ccagttctgc aatcgcgcgt accgccgcca gtttcatctc ttcgttgatg gcggttgcgc
    45721 caacgtccag cgcgccacgg aagatgaacg ggaagcaaag aacgttgttc acctggttcg
    45781 ggtagtcgga acggccggta cagatgatgg catccggacg cacttctttc gccagcggcg
    45841 gcagaatttc cggttccggg ttcgccagcg ccaggatcat tggtgcccga gccattttct
    45901 tcaccatttc ctgggtcagc actttcgggc cggaacagcc caggaaaata tccgcacctt
    45961 caatcacatc atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt
    46021 ccgccatatt tggctcacgg ccctgataga taacgccttt tgaatcgcaa accacgatgt
    46081 tatgtttttg cagccccagc gctaccagca ggttcataca ggcgattgct gcggcacccg
    46141 cgccggaaac caccatccgc acgtcggaga tgtttttctc caccacgcgc aagccgttga
    46201 ggatggcggc agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat
    46261 tcatccgctc gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga
    46321 ggttgatgcc gccgaaggtc ggttcgagcg cggcgacgac ttcaataaat ttgtccgggt
    46381 cgagttcatc aacttcaatg tcaaatacat caatcccagc gaatttctta aatagaacgc
    46441 ccttgccttc catcaccggt ttgcctgcca gcgcgccaat gttgcctaac cccagcaccg
    46501 ccgtaccgtt agagatcacc gcaaccaggt taccgcgcgc ggtatatttg taggctttta
    46561 gcgggtcttt ttcgatttca agacaaggtg cggcaacgcc tggtgagtag gccagcgcca
    46621 gatcgcgctg cgttgccaga ggcttggttg gagaaacctg gattttccct ggaactggaa
    46681 attcatggaa atcaagtgca ctttgtttta actggtcatc catttggttg ttcctttcac
    46741 gtaacgttca caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta
    46801 ttaccctgcc ggagtctaca gactttgagc aagtccaaac tctcaccatt aatataatgt
    46861 tttggtaata atcctataac actgatgtta cctgcttaat ccagcaatac catgcctgtc
    46921 tgctatgctt ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta acgcactcat
    46981 ctaacacttt acttttcaag gagtatttcc tatgaacgag ttagacggca tcaaacagtt
    47041 caccactgtc gtggcagaca gctgcgatat tgagtccatt cgccattatc atccccagga
    47101 tgccaccact aatccttcgc tgttactcaa ggctgccgga ttatcacaat atgagcattt
    47161 aatagacgat gctatcgcct ggggtaaaaa aaatggcaag acccaggaac aacaggtggt
    47221 cgcagcgtgt gacaaactgg cggtcaattt cggtgctgaa attctcaaaa tcgtacccgg
    47281 ttgcgtgtca acagaagttg atgcacgcct ctcttttgat aaagaaaaga gtattgagaa
    47341 ggcgcgccat ctggtggact tgtatcagca acaaggcgtt gagaaatcac gcattttgat
    47401 caaactggct tcgacctggg aaggaattcg cgcggccgaa gagctggaaa aagaaggtat
    47461 taactgcaac ctgacgctgc tgttttcttt tacccaggcg cgggcctgtg cggaagcagg
    47521 cgtttttctg atttcgccgt ttgtagggcg tatttatgac tggtatcagg cacgcaagcc
    47581 gatggacccg tatgtggtgg aagaagatcc gggcgttaaa tcggtgcgca atatctacga
    47641 ctactataag caacaccact atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga
    47701 acaaatcctc gccttaaccg gctgcgatcg actgactatc gcaccgaatt tgctgaagga
    47761 gctgcaggaa aaagtttctc cagtggtacg taaattaatc ccgccttctc agacgttccc
    47821 acgcccagca cccatgagcg aagcggagtt ccgttgggag cacaatcagg atgcgatggc
    47881 ggtagaaaaa ctgtctgaag gcattcgtct gttcgctgtt gatcaacgca aactggaaga
    47941 tcttcttgcc gccaaactat aaaccagcca cggagtgtta tatgtcccga aaagaccttg
    48001 ccaatgcgat tcgcgcactc agtatggatg ctgtacaaaa agccaattct ggtcatcccg
    48061 gcgcgccgat gggcatggct gatattgccg aagtgctgtg gaacgatttt cttaaacata
    48121 atcctaccga cccaacctgg tatgatcgcg accgctttat tctttccaac ggtcacgcgt
    48181 cgatgctgct ctacagtttg ctacatctga ccggttacga cctgccgctg gaagaactga
    48241 agaacttccg tcagttgcat tcgaaaaccc caggccaccc ggagattggc tatacgccag
    48301 gcgttgaaac caccaccggc ccgcttggac aaggtttggc gaacgccgtc gggctggcga
    48361 tagcagagcg tacactggcg gcgcagttta accagccaga ccatgagatc gtcgatcact
    48421 tcacctatgt gtttatgggc gacggctgcc tgatggaagg tatttcccac gaagtctgtt
    48481 cgctggcagg cacgctggga ctaggcaagc tgattggttt ttacgatcac aacggtattt
    48541 ccatcgacgg tgaaacagaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct
    48601 atcactggca tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga
    48661 tccttgaagc gcaaagcgtg aaagataagc cgtcgctgat tatctgccgt acggtgattg
    48721 gctttggttc gccgaataaa gcaggtaagg aagaggcgca cggcgcacca ctgggggaag
    48781 aagaagtggc gctggcacgg caaaaactgg gctggcacca tccgccattt gagatcccta
    48841 aagagattta tcacgcctgg gatgcccgtg aaaaaggcga aaaagcgcag cagagctgga
    48901 atgagaagtt tgccgcctat aaaaaggctc atccgcaact ggcagaagag tttccccgac
    48961 ggatgagcgg tggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt
    49021 tacaggcaaa tccggcgaaa atcgctaccc gtaaggcttc gcaaaatacg cttaacgctt
    49081 acgggccgat gctgcctgag ttgctcggcg gttcggcgga tctggctccc agcaacctga
    49141 ccatctggaa aggttctgtt tcgctgaagg aagatccggc gggcaactac attcactacg
    49201 gggtgcgtga atttggcatg accgctatcg ccaacggcat cgcgcaccac ggcggctttg
    49261 tgccgtatac cgcgacgttc ctgatgtttg ttgaatacgc ccgtaacgcc gcgcggatgg
    49321 cggcactgat gaaagcgcgg cagattatgg tttataccca cgactcaatt ggcctgggcg
    49381 aagatggtcc gacgcaccag gctgttgagc aactggccag cctgcgctta acgccaaatt
    49441 tcagcacctg gcgaccgtgc gatcagatgg aagcggcggt gggctggaag ctggcggttg
    49501 agcgccacaa cggaccgacg gcactgatcc tctcaaggca gaatctggcc caggtggaac
    49561 gtacgccgga tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa gacagcggcg
    49621 gtaagccaga tattattctg attgccaccg gttcagagat ggaaattacc ctgcaagcgg
    49681 cagagaaatt agcaggagaa ggtcgcaatg tacgcgtagt ttccctgccc tcgaccgata
    49741 ttttcgacgc ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc
    49801 gcgtggcggt ggaagcaggt attgccgatt actggtacaa gtatgttggt ctgaaagggg
    49861 caattgtcgg gatgacgggt tacggggaat ctgctccggc ggataagctg ttcccgttct
    49921 ttggctttac cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg
    49981 cctgatggtg atcgccggat gctgtatgtt cccctcaccc taaccctctc cccagaaggg
    50041 cgaggggacg gtccgtgcgc tttttagggc cgcatctgcg attttgttta acgggtgatc
    50101 cacaacgtgg gcctggcgtc tggcccatgc cagttatcgc aggtaggttc tgcggcgtaa
    50161 cgcaccaggc gaaaacgctg accgtcaaag cgccagcgcg cctgaatgcc acaatcgctt
    50221 aatccgcgcc ctttcgctaa ggtcaccagt tcacgcgatt tctcatcaaa tgttgcgttc
    50281 atcagttcca gttcattcgt ctcctggccg ttgttgaacg gcaaacgcaa ccgaaccggg
    50341 cgcgaggcta gcggcttttt acgcgacaca atccatgcca aatcaatggt gttataggcc
    50401 cctgcctcac agctaatcat catcagtgct ttatcatcgg tcagcgcagt gacattcacc
    50461 tcacgacgta acggatcaag cgagcagcgc agaccattca tccgccagtt tccataatcc
    50521 agcaaatcgt tacgttcttc gagtgagagt ggcgtcggcg ttgggttaac caccgcgacc
    50581 tctttcagcg caggcgcggg cggtacgctg agaggcggtt cgtccccttt cttgatccac
    50641 gcggtttcac tgccaacgcg cttttgctga gcatcaataa acaacaacgc cgcttttaag
    50701 ccgctcagag aaatggtctg attgccgtcg cgtaaggtga tcgccttccc ttcctgaatt
    50761 atctgcaaaa acgcggtgat ggttgccgta tcatcggtta ctaataacca tggtgaaatc
    50821 cgccacttgt caccacttaa tgccaacggc tcaccatcta atagcagccg tggcgctatc
    50881 tccccttcag acgcatccgg cgacttcaac ccaccgcgct caatacgtaa aacggcatcg
    50941 gtatgcgccc cagcgctgcg gctcagggtc atcaccagtc cattatgatc gcccgtattg
    51001 cgcgccacac aaaaattttg attattacag gtgacctgcc agtcggaaaa cgcccgttgc
    51061 gctggtgccg cccacaccaa agacgtcggc aacaaagcga aaaaaaagag aaggaaaatg
    51121 cgatagcgca tggacggtac gaccccagaa gttcaaacaa acagcacaag tcgtatcttc
    51181 gttgcgcagg tagcgcggct caatcggatt tgtcggattg agccaataat aaacaacgaa
    51241 atattattgc tatttttcag tccattaaat gtgacatttg caaatagtta agcaataaca
    51301 ccgtcttacc gtcacgtatc tcgccggttt tgatcatctc cagcgcctgg ctgaacggca
    51361 gctcgagcac ttcaatatct tcatcttcaa caccgccacc ggcgttagcg cgctgattgt
    51421 cactgtattc ggcgataaaa aagtggatta gctcagtcac accgccaggc gacatataca
    51481 gttcaaataa tttgcgcact tcgcccactt catagcccgt ctcttcaatc gcttctttac
    51541 gaatgcacac ttccggttcg tcgttatcca gcagcccggc gcaggtttca atcagctgcc
    51601 cgctttcatt gccattaacc caggtagcga cacggaactg acgaatcaga accacgctct
    51661 ttttcttcgc gttgtacagg aggatcgtcg caccattacc gcgatcgtaa acttcacgtt
    51721 tatggcggat aacttcgcca tctttgcgcg tgagatcgta agtaatgttg tgcagggtga
    51781 aatagttatc ggagagaatt ttgtctttaa tgagggtgat ttgttgcgtc ataccgactc
    51841 cacagcgcga aatgaacaat tatattacgc tgtgaagtcg ggtttgtctg cgcaggctat
    51901 cagtgagatt tgactgattt tacccccagc cagtcaataa tcccctgtgc cgcatgacgt
    51961 ccttctgcca ttgcggtaac caccagatcc gcaccacgca cggcgtcgcc accggcgaag
    52021 attttcggat tggtggtctg gtaacggtac tggctttcca catccgcgat gatgcggccc
    52081 catttgtcta ccgttacacc gtgcgactcc agccacggca tcccgtgtgg attgaagcca
    52141 aacgccataa tcaccgcatc ggctggcatg acaaattcac tgccctcaat cggtacaggt
    52201 cgccgacgcc cctgggcatc cggctcgcca agacgcgtgc gcaggaaacg aattccgcaa
    52261 acgtgacctt gctcattcag ctcaagcgcc accggctgga cgttaaattc gaagttggcc
    52321 ccctcttcgc gggcgttctt cacctctttc ttcgagcctg gcatgttagc ttcatcacga
    52381 cgataagcgc aggtgacgtt actcgcgccg tggcgcagcg cagtacgcac acagtccatc
    52441 gcggtgtcgc cgccgcccag taccacgacg ttaagtccgg cggtattgat aaacggctct
    52501 tccggtagct cttcgagccc catcacctgt ttagtgttgg caatgaggaa cggcagcgcg
    52561 tcataaacgc ccggcgcatc ttcattgggt aaacccgctt tcatggaacg gtaagtgcct
    52621 acgccaacga agaccgcgtc gtattgttcc aaaagcaaat ccaaagagac atctttaccc
    52681 acttcacaat tgagttcgaa gtgaatcccc atcgcgctga agatttcccg acggcgtgcc
    52741 agcagggatt tatccagttt gaaagaagga atgccgaaag tgagcaagcc accgatttct
    52801 ggatggcgat cgtacaccgt caccgccacg ccattgcggg tcagaacatc cgcacaggcc
    52861 agccctgccg gacctgcccc gataatcgcc acccgcttgt ccactttggt gacatgactt
    52921 aagtcaggac gccaaccttt cgccaacgcc tgatctgaaa tatagcgttc aatgttgccg
    52981 atagttaccg cgccgtgctc atcgcgaata gtacaggcac cttcacacaa acggtcttgc
    53041 ggacaaacgc gtccggtaat ttccggcagg gtgttggtct ggtgagaaag ctcgacggcg
    53101 gcgtcgatgt ttccggcttt caccagttca atccactgcg gaatatggtt atgcagcggg
    53161 caggtccatt cacaaacgct gtgctcgccg aacttaaggc agcgcgaggc ttcccgttgt
    53221 gcctggtcgg cgcgaaatgg cagataaatt tcatcaaaac cggttttgcg cgcttcaatc
    53281 gccagtttat ccggctcgcc acgcgcgggc gttgcctgca tttgttcgac tttactcatt
    53341 accggcattt cttgtgcagc ggtactggca tgccacggtt gatgctcctg acgtgcggtg
    53401 cgcaagcggc gggatttcgc catgccggag agtgcgacgt cagtgaccag ttgcagcgcg
    53461 tccgccgggc aattctcgac acaggcagga ccgttttcgc gccctgcaca aaggtcgcat
    53521 ttatgcgccg tggcttttac ttttcctgcc gcgacgggtg tcaggacgat ttgcatcgta
    53581 ccaaacggac aggccaccac gcaggattta cagccaatac acttttgctg attgacctga
    53641 atgctgtcat caacgtggct gattgcgcca ttagggcagc tacgggcgca gggcgcatct
    53701 tcacaatggt gacaggtcac tgcactacgt tgctgctgat gtttgataac cgtaattcgg
    53761 ggatgaaaat ggtgttggct caggacatgt tgctcatcat tgtgagccat gacacaggcg
    53821 atttcacaag catgacaacc cagacattgc tgactgttgg ccataataaa acgattcata
    53881 acgaccttct tttttggttg taaaaacctt attctttata tgagtgttgt tattacccga
    53941 cttacagggg aaccggcaat gttcatgtgc ccagaataag taactatttc gctataaact
    54001 gtggcagatc aaataatccc atcactgact aaattgcgtt tcagcgaact ggaacattaa
    54061 tgattttttg tggagaagac gcgtgtgatt gttaaacgac ccgtctcggc cagtctggcc
    54121 cgggcctttt tttacattgt gctgctgtcg attctttcca cgggtatcgc tctgctaact
    54181 ctggcgagca gtttgcgcga cgctgaggct atcaatattg ccggatcgct gcgtatgcag
    54241 agttaccgcc tgggctacga cttgcaaagt ggcagtccac aactcaatgc acatcgccag
    54301 ttgtttcagc aggcactgca ttcaccggta ttaaccaacc tcaacgtctg gtatgtgcca
    54361 gaagcagtaa aaactcgcta tgcgcatctg aatgccaact ggctggagat gaataatcgg
    54421 ctcagcaagg gcgatttgcc gtggtatcag gccaatatta ataattatgt taatcagata
    54481 gacctgttcg tgctggcttt acagcactac gctgaacgca aaatgctgct ggtggtggcg
    54541 atttccctgg ctggcggtat cggtattttc acgctggtct tttttacttt gcgccgcata
    54601 cgccatcagg tggttgcccc gctgaatcag ctggttaccg ccagtcagcg tattgaacac
    54661 gggcagttcg actcgccgcc gctggatacc agtctgccga atgagcttgg tctgcttgca
    54721 aaaaccttta accaaatgtc gagcgagctg cataaattgt acctttcgct ggaggcgtca
    54781 gtagaagaaa agacccgcga tctccacgag accaagcgtc gtctggaggt gttgtatcag
    54841 tgcagtgttc ccaggcgctg aacaccagcc agattgatgt gcattgtttc cgccatattt
    54901 tgcagattgt tcgcgacaat gaagcagctg aatatctgga gctgaacgtt ggggataact
    54961 ggcggattag cgaagggaag ccaaaccctg agctgccaat gcagatttta ccggtgacaa
    55021 tgcaagagac ggtttacggc gaactgcact ggcaaaatag tcacgtttca tcatcagaac
    55081 cgctgcttaa cagtgtttca tcgatgctgg gacgcggttt gtactttaat caggcgcaga
    55141 agcattttca gcagttattg ttgatggaag aacgtgcgac catcgcccgc gaattgcacg
    55201 actcgctggc tcaggtgctt tcttacttac gtatccagtt gacgttactg aagcgttcga
    55261 taccggaaga taacgccacc gcacaaagta tcatggccga tttttcccag gcgttgaatg
    55321 atgcttatcg gcagttacgc gagctgttga ctactttccg cctgacgctg cagcaggcgg
    55381 atctcccctc cgcgttgagg gaaatgctgg atacgttaca aaatcaaacc agcgctaaac
    55441 tgaccctcga ctgccgtctg ccaaccctgg cactggatgc gcaaatgcag gtgcatttgt
    55501 tgcaaattat tcgcgaagcg gtgctgaatg cgatgaagca cgccaacgcc agcgaaatcg
    55561 ccgtcagttg cgtcaccgcg ccggacggca atcacacggt ttatatccgt gataacggga
    55621 ttggtatcgg tgaaccgaaa gaacccgaag gtcattatgg actgaatatc atgcgcgaac
    55681 gcgcggaacg gctgggcggg acgctgactt tttcacaacc ttccggcggc ggcacgttag
    55741 tgagtattag ctttcgctcc gcagagggtg aggggaaggg gaacacgccg gctgaggtgt
    55801 aaataattac cctcacaccg gccctctccc tttaaaggcg agagggaaaa acgtgcccat
    55861 attgacctgt aagcgagtgt gtcagcaatc tcatgataat ttacattaac tccttttttt
    55921 ctccacgatt ggctcgtacc ttgccgctac agtgaagcaa gtcaagccta caacaatacg
    55981 cagaaacacg aggtcctctt ttaatggcga atttctttat tgatcgcccc atttttgcct
    56041 gggtgctgac aatcctgttg tgtctgacag gtaccctggc gattttttca ttgcccgttg
    56101 aacaataccc cgatctcgcg ccaccgaatg tgcgagtgac cgctaactat cccggcgcat
    56161 cggcccagac gctggaaaac accgtgaccc aggttatcga gcaaaatatg accggcctcg
    56221 ataatctgat gtatatgtca tctcagagca gtggcaccgg tcaggcatct gtcactttaa
    56281 gttttaaagc aggcaccgat ccggacgaag ccgtgcagca agtacaaaac cagctgcaat
    56341 cagccatgcg aaagttaccg caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg
    56401 gcgataccaa cattctgacc attgccttcg tctctaccga tggctcgatg gataaacagg
    56461 atattgctga ttatgttgcc agtaatattc aggacccgtt aagccgcgtg aatggcgtcg
    56521 gggatatcga tgcctatggt tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac
    56581 tcaacagttt ccagatgacg gctaaagatg tcactgatgc cattgagtca cagaacgcgc
    56641 agattgcggt tgggcaactt ggtggtacac cttccgtcga taagcaggcg ctcaacgcca
    56701 ccattaacgc ccagtcactg ctgcaaacac cagaacagtt ccgcgatatc accttgcggg
    56761 tgaatcagga cggctcagag gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg
    56821 agaaatacga ttatcttagc cgcttcaatg gtaagccagc ctccgggttg ggggtaaaac
    56881 tggcctccag cgcaaacgaa atggcgacag cggagctggt gctcaatcgt ctcgacgagc
    56941 tggcgcagta tttcccgcac ggactggaat acaaggtggc gtatgaaacc acctcgtttg
    57001 ttaaagcctc cattgaagac gtggtgaaaa cgctgctgga agctatcgct ctggttttcc
    57061 tcgttatgta tctgttcctg caaaacttcc gcgccacgct gatacccact atcgccgtgc
    57121 cggtggtgtt gatgggaacc ttctccgtac tttacgcctt cggttacagc gtcaacacct
    57181 taaccatgtt cgcgatggtg ctggcgatcg gtctgctggt ggatgacgcc atcgtggtgg
    57241 tggaaaacgt cgaacgtatt atgagtgagg aaggactcac tcctcgcgaa gccacacgta
    57301 aatcgatggg gcagatccag ggggcactgg tcgggattgc gatggtgctt tcggcggtat
    57361 ttgtaccaat ggccttcttc ggcggcacca ccggtgccat ctatcgccag ttctctatta
    57421 ccattgttgc ggcgatggtg ctgtcagtac tggtagcgat gctcctcact ccggctctgt
    57481 gtgccacgct acttaagcca ctgaaaaaag gtgagcatca cgggcaaaaa ggtttttttg
    57541 cctggtttaa ccagatgttt aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa
    57601 ttctccaccg tagcctgcgc tggattgtga tttatgtcct gctgcttggc ggcatggtgt
    57661 tcctgttcct gcgtttgccg acgtcgttct taccgctgga agaccggggc atgtttacta
    57721 cctcggtaca attgcccagc ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa
    57781 tcgagaaata ttacttcacc catgaaaaag acaacatcat gtcggtgttt gccaccgttg
    57841 gttctggccc tgggggtaac gggcaaaacg tggcgagaat gtttatccgc ctgaaagact
    57901 ggagcgaacg cgacagtaag accggcacct cgtttgccat tatcgagcgt gcaacgaagg
    57961 cgtttaacca aattaaagaa gctcgcgtta tcgccagcag cccaccagca attagcggtc
    58021 ttggtagttc tgcaggtttt gatatggagt tgcaggacca cgctggagcg ggtcacgatg
    58081 cgctgatggc agcacgtaat cagttgctgg cgctggcggc ggaaaacccg gagctaaccc
    58141 gtgtgcgcca taacggcctc gatgacagtc cgcagttgca gattgatatc gaccagcgta
    58201 aagctcaggc gctgggcgtt gatatcgacg atattaacga cacactgcaa accgcctggg
    58261 gttcgagcta tgtgaatgac tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg
    58321 cagctgcgcc gtatcgcatg ctgccagatg acatcaatct ctggtatgtc cgaaataaag
    58381 atggcggcat ggtgcccttc tctgctttcg cgacctcacg ctgggaaaca ggctcgccgc
    58441 gtctggaacg ctataacggt tattctgcgg ttgagattgt tggggaagcc gcaccggggg
    58501 tcagtaccgg tacggcgatg gatattatgg aatcgttagt gaagcagctg ccaaacggct
    58561 ttggtctgga gtggacggcg atgtcgtatc aggagcggct ttccggcgcg caggctccgg
    58621 cgctgtacgc catttccttg ctggtggtat tcctgtgtct ggcggcattg tatgaaagct
    58681 ggtcggtacc gttctcggta atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg
    58741 caacctggat gcgcgggctg gaaaacgacg tttacttcca ggtgggcctg ttaacggtca
    58801 ttggtttatc ggcgaaaaac gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa
    58861 aaggccatga cctgtttgaa gcgacgctcc acgcctgccg tcagcgttta cgcccgattc
    58921 tgatgacctc gctggcattt atcttcggcg tattgccaat ggcaaccagc acgggtgccg
    58981 gttccggtgg tcagcatgcg gtgggtactg gcgtaatggg cgggatgatt tcagctacta
    59041 ttctggctat ttacttcgtg ccgctgttct ttgtgctggt gcgccgccgc ttcccgctga
    59101 agccgcgccc ggaataagca ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact
    59161 ttccgattaa gaacctgctc agcgggttct tgctgtttat actttgtctc aggaattact
    59221 tacgaagcat aacttcgata aagtctttcc agttccccag ttcacgttca atcataacaa
    59281 cctctcttat aattatgggt attctacgga aacaatatac cgtggtgaag ctaatttact
    59341 cgattgctgc gatgactacc tccgggggac aaaccttatg taaatactat ggtcctgcgg
    59401 tgataatttg tatgtgatac acagcaacat ttcgagatat tcatacggca tctaatactg
    59461 atttaattct ggttaaaata cagacagata acaagatgaa tattcttaat gtttacgtta
    59521 aaaatgttta atattattta atagttgtta atttgaatac ttcgataatg ttatattttc
    59581 tgataatcat ttgcaggcaa aatgttttca cccttaaatg agtatttatt ctcataaatc
    59641 gaaaaaggat tcattatggt tacactttac ggcatcaaaa attgtgacac cattaaaaag
    59701 gctcgccgtt ggctggaagc caataacatc gactatcgtt ttcatgatta ccgcgtcgat
    59761 gggctggaca gcgaattatt gaacggtttt atcaacgaat taggctggga agcgttactc
    59821 aacacccgtg gtacaacctg gcgtaaactg gacgaaacta cccgcaataa aatcaccgat
    59881 gcggcctctg cggcggcatt aatgactgaa atgcctgcaa ttatcaaacg tccattgctc
    59941 tgcgcgcctg gtaagcctat gctgctgggt ttcagtgatt ccagttatca gcaatttttc
    60001 catgaggtgt agtctatgtc gtgcccggtt attgagctga cacaacagct tattcgccgc
    60061 ccttccctga gtcctgatga cgcaggatgt caggctttgt tgattgaacg tttgcaggcg
    60121 atcggtttta ccgttgaacg catggatttt gccgatacgc agaatttttg ggcatggcgt
    60181 gggcaaggtg aaacgttagc ctttgccggg cataccgacg tggtgccgcc tggcgacgcc
    60241 gatcgttgga tcaatccgcc gtttgaaccc accattcgtg acggcatgtt attcgggcgc
    60301 ggtgcggcag atatgaaagg ctcgctggcg gcgatggtgg tagcagcaga acgttttgtc
    60361 gcacaacatc ccaaccatac ggggcgactg gcatttctga tcacttctga tgaagaagcc
    60421 agtgcccaca acggtacggt aaaagtcgtc gaggcgttaa tggcacgtaa tgagcgtctc
    60481 gattactgcc tggtcggcga accgtcgagt atcgaagtgg taggtgatgt ggtgaaaaat
    60541 ggtcgtcgcg gatcgttaac ctgcaacctc accattcatg gcgttcaggg gcatgttgcc
    60601 tacccacatc tggctgacaa tccggtacat cgcgcagcac ctttccttaa tgaattagtg
    60661 gctattgagt gggatcaggg caatgaattc ttcccggcga ccagtatgca gattgccaat
    60721 attcaggcgg gaacgggcag taacaatgtt attccgggtg aactgtttgt gcagtttaac
    60781 ttccgcttca gcaccgaact gactgatgag atgatcaaag cgcaggtact tgccctgctt
    60841 gaaaaacatc aactgcgcta tacggtggat tggtggcttt ccgggcagcc atttttgacc
    60901 gcgcgcggta aactggtgga tgcggtcgtt aacgcggttg agcactataa tgaaattaaa
    60961 ccgcagctac tgaccacagg cggaacgtcc gacgggcgct ttattgctcg catgggggcg
    61021 caggtggtgg aactcgggcc ggtcaatgcc actattcata aaattaatga atgtgtgaac
    61081 gctgccgacc tgcagctact tgcccgtatg tatcaacgta tcatggaaca gctcgtcgcg
    61141 tgatgagtgg ttctgcaaga ggaaataagc atggactggc tggctaaata ttggtggatt
    61201 ctggtgattg tctttttggt aggcgtcctg ctgaacgtga ttaaagatct caagcgcgtc
    61261 gaccataaga aatttctcgc caacaagccg gagcttcccc cgcatcgtga tttcaacgat
    61321 aagtgggacg atgacgacga ctggccgaaa aaggatcaac cgaagaagta agctgagtgc
    61381 tcaaccgtag gccggataag gcgttaacgc cgcatccggc aatggtgaac gatccctgat
    61441 gcgacgctca cgcgtcttat caggccaggt aatctgtctt ttacatcatt tcaatcacat
    61501 catcatcgcc aggcttaccg ccacttagcg cttggtcgaa ataatgtttc ggaatggtat
    61561 aacgcaagtg atccagggca aattgcatac tgcgattgtc gatcgcatgg cctaaatcct
    61621 ccacgatatc cagcgtcacg tcaccaccgg cactgattaa cgcttcctgc gccgcaactg
    61681 catgcgccag atcgataacc ggatcttcac caccatgaat caagtgaatt gtcgtggctg
    61741 tagacgcggt ctccggcagg ctcgcatagc gtccgttaaa ggcaatgacg cgcgaagcaa
    61801 ggcccggttc ggctttaatg ctctccagcg ccataatcgc gccttgtgag aaaccaatta
    61861 gcgccgtggc gttagcccct actccgctct gtttctgcca gtagcgtacc gtttcaataa
    61921 acgtcggcat gatggcatcc acacgcgcct ggcgattatc ttccgtaata ccctgcactg
    61981 aaaaccactg acgccccgcc ggattaccgc tcggctccgc gccaccgaca ctcaccacca
    62041 gcgcatcagg aaacagcggt gcaaaccagc tgcctatttc ccccattgcc accgggttat
    62101 ccccgacacc gtggaaaagc agtaacaact gttgtgcagg tttatccggg ctttgaacaa
    62161 caaaatggtc atgtttcatg gcgaactcct taactgatgt cattaatttt acgccgctga
    62221 ctcacaatga ccatgggaga aaattgaagg agtcagtgaa aaaattgcca ttgcgttatg
    62281 cgatcgcgca gacgctcagt gcgaacctca tttagtgcga acagcgcctg cgcggcctct
    62341 tcccgctggc gaaccagtaa catcttgcgg cctgaaagtt taagtgtggt acataactgc
    62401 gcgtcactgg cgtttttctg taaacgccca cgcagcgccg gtaatgccag ttcactggtt
    62461 tgtagcagac gcaataagca acctaacgat gttaatagcg gacgatgagc gaaagcaaaa
    62521 ccggccagtt caagccagtc gtcgtcagaa aggatggcat cgtttagtgg atcaacagga
    62581 agcatttcgc cattccactg cgcgagagct tgcgcatcgc gacgtaaacg gtaatgctca
    62641 cgttcagcca gctgtttacc cgcatcactc atcggtaaca gcgccatcgc cgtatagcaa
    62701 ccgctgctgg cttcccgatg attacccatc cgcaccagca caaaaccgca gcgttgccag
    62761 aaacgccata actccccagt gtaaccaaaa ctcaccgaaa gatagtcgag gtcatgcgta
    62821 tattgcaaag ctccagcaat aagttgtcgc cctgtgcctt cccgctgacg agccggatga
    62881 actgctatcc ggctaacccg ccttccgcgc aatgtcgccg ccagtggatt gctgccgtgc
    62941 gccgccagcg actgggccac cagattaccc cgcgggcgac gaaaacctgc ccataccgcc
    63001 tgactgagtt cttgagataa tccgccctca tccaccagcc acaacgcccc ggcaatctcg
    63061 ttttcgccag ccgcctgtaa aaaatgttgc cctggtgcat ccatcatccg gcgtaaatcc
    63121 agcggcgagg tccggtagtg cgcaccagac aataactgat aaacctttaa cggcgtttct
    63181 ggctcgcttc gccataacgt ttgttcaaat gcggaaatga cgatattgcc ttgtggtgta
    63241 tgggtgaagt tttcatcgtt aaaaaccagt gcctcactaa ccattttttc cagcgggcat
    63301 ccctgtgccc agcggatcgg ctgttgcagt tcaaaacggt gtaaatgcgg aaagcgagcg
    63361 caaaatttca gcaaaaaacc acgtccggta ccttcgtagc cctgcaccgt agtggttaac
    63421 aacgttcgag gaaaacgcga taccagttga tgcaacaacg gcgcaggtat ggctgcggct
    63481 tcgtcgacca ccagccagtc ggcttgctca tcgctggcta acaaggcatc cggtgcaata
    63541 aagcgaaact tctcgcccgc aaattgtgcc agtacatccg ttgccgcttt tgcgggcgcg
    63601 gtgacaatcg cactgcccgc aatacgagaa atgagttgcc ctgccaacgc cgacttaccg
    63661 cgcccacgcg cagccgttac cgctgccacg cccggcggca tggtcagtag ctgctgtaag
    63721 agttgctgtt gttctggttg tggtgcgccg gtcgcggggt gccagtcagt acggggagta
    63781 aaatgcgcca acgagaacgg ctggttttgc cgccagagga tagcgtcgtt atccgccgta
    63841 agtacgcgtt tgagatgctg gacaaaatgc ggcgtcgcaa tagggtcagg gcaatcactc
    63901 cagcgcagcg agtcggcatc aggttggttt tcccactctt cccatacagg gagtaacaaa
    63961 accagccagc ttcccgcttt caacgttccg ctaagtgctg caaaggcagc ggcatcaaag
    64021 ccgtggcggg cgtcgaatac cgcatgccgg aactcgcgcc caagtaaagt ttgtagcgcc
    64081 gagggtgaac agtggttttc agcatctagc tgcggcgaaa tccacagcca gtcgccaggt
    64141 aaggcatcac gcaacttaag agcatgatca aaacaccaac cctcttcccc gctcaacacc
    64201 agcaagcggc agatcccttc acgtttcatt tgcgctgtta atgtgtaaag cgcagtcagt
    64261 tcagccatcc ctgccccgga aattaaatgc ttttaccaaa agtattgcat tgtgccggat
    64321 cgccgctgtc gaaaccacgt ttaaaccagc tgtagcgttg ctgagaagtg ccatgtgtga
    64381 aactgtctgg tactactcgc ccctgacttt gctgttgtaa acggtcatcg ccgatggcct
    64441 gcgccgcatt cagcgcctct tccagatcgc cagtttccag aacgccttgc tgctgcatac
    64501 tatgccccca gacaccggca aaacagtcgg cctggagttc catacgcaca gataagcggt
    64561 ttacttccgc ctgcgtcgcg ttttgttgca gttgacgaac tttcggctcg atgcctaaca
    64621 gtttctgcac atgatgaccg acttcatggg cgataacgta cccctgggca aaatcgccat
    64681 ccgcgccaag tttgtctttc atgtcatcat agaaggagag atcgatataa actgtgccat
    64741 ccgccgggca atagaacggc cccataatgg actggcccgc cccgcagccg gtgcgcgtca
    64801 ttccacggta catgaccagc ttcggttgct gataggtctt acccatcttt tcgaactgtt
    64861 gtccccaggt gtcttccgtg gttgcctgaa tcaccgaggt gaattttgcg gcttcgtctt
    64921 cattagggct aattgaccgc gttgattgtt gttgagaaac cggctgcccg gtcatcaacc
    64981 cggttaaatc aacgccatag tagcctgcaa ccagcacgac tatcagtaaa atcagcccgc
    65041 ctttaccgct cggcagacgg aaaccgggac cgcccattga cggaccgcca gagctgttgc
    65101 gcctgtcttc aacattgtca ctttcacgtc gcccttgcca acgcatagat acctcaacaa
    65161 tatattcatt atagaaatga tcgtaggtgg ttcagaggaa gattaccaca ggaaagcggg
    65221 atgaaatggc ggaggaatga gcgttcaatg catatattcc aagagcggtt gtcttgccgt
    65281 gccagctgca cggcaagatg atgaacagaa aaatcagtcc agctgtacac ccaggcggcg
    65341 ggcgacggct tcataggctt cgatcaggcc accgaggctc tggcggaaac ggtctttgtc
    65401 cattttctcc agtgtttctt tgtcccacag gcggctgccg tccggggaga actcatcgcc
    65461 cagtaccact tcacctttgt acagaccaaa ctccagcttg aagtcgacca gaatcagacc
    65521 agcatcatcg aacagttttt tcagcacgtc gttcgctttg taggtcagct ctttcatacg
    65581 cgccaggttc tctttgctca cccagccaaa ggtttcgcag taagattcgt tgaccatcgg
    65641 atcgtgcatg gcatcgtttt tcaggaacag atcgaacagc ggcgggttca gctcaatacc
    65701 ttcttcgatt ccaagacgtt tcactagaga gccagcagca cggttacgca cgacacactc
    65761 aaccggcacc atatccagct ttttcaccag acattcggta tcggagagca gacgctccat
    65821 ttgagtcggg atacccgctt cagccagttt gctcataatg aagtagttga acttgttgtt
    65881 caccatacct ttgcgatcaa actgctcaat gcgcgcgcca tcccctgctg acgtatcatt
    65941 gcggaattcg agcaccaaca ggtccgggtt ttccgtgctg tatacggttt tcgctttacc
    66001 acgatacaac tcagcttgct tttgcatctt tatcactcct gggtgtgaat taacgtttta
    66061 aaatcttttg ctgtctggtg tgccggatgt tttgtcggat gcggcgtgaa cgccttatcc
    66121 gaactacgtc cgacaaacaa atttcgtgcg aattaccgct aaaatcgcgt tttcctgccg
    66181 acgcacacgt ttgcgtatca tatcagaaaa aagggccgga tgattccagc cctgtatttt
    66241 tacttgctaa acgcagcctg gaagacagct accagcgcgt cgttctgact ctgagtcaga
    66301 gtatgacctt tcggatcgat gaactgtagg ctgctgcggt tatctaaatc gccaacctgc
    66361 agtttatagt caccggatgc caggcctgga tcgctcgcgc ccagttcctg ccagtcgctg
    66421 tcagacagcg gcttataagt tacggccatg ttgccctgcg aacgggtgct gtcggtcact
    66481 ttcatgccca ctttttccag cgccgctggc agacgttgcc aaaccacatt gaacggcccg
    66541 cgtacgacca gcattggtaa accggtgtca tcagctgcac tttgtacgtc catagtggtg
    66601 gaggcacgat tttgcgcagc gttcgcggcg tcagtggcag atttatccag accggcggaa
    66661 ataacgttca tcatctccgt gctgtaacgc tgcatggaag ccgcgtctgc aaccggtttg
    66721 cccgcctgtt ccaggttcag cagtttaacc gtaaccgcct gctgataacc ctgcggctta
    66781 acagagattt gataacgacc acgatactgc tcgtcttcgt ccagacggtt ccattgtacc
    66841 caatcggtgg tcagtgtctg accagcatca tcacgttggg tgatggtgta gtttttcgcc
    66901 tgcagcacgc taaccacctg cggccacaga gtattgccac gaccattttc caccagcaat
    66961 gaagcggtat cgcccgtgaa ctgggtacgc gcgccagaaa ccagtgccaa cggctgggct
    67021 ggtggacgaa tgtccagcgc cttaccgaca gcaccactac cgttggtcac cgggattgca
    67081 taatcaccgg aggtcaccgg caaaatcatt ccagccgggg catgaagctc cgcaagcggt
    67141 gccgcttcca ggtaggcttc atcaccactg acctgacgct tatagcgtga gtcagaacta
    67201 caggcagcga gtaataaaac aagcgaaaca cccgcaacct ttgccaggcg cgacttttga
    67261 acagagtaag ccatcaaatc tccctaaact ttacagcaaa ccggcatgct taagcgccgc
    67321 tctgaccgtc tcacgaccac tgtcggtgat tggtgtcatt ggcaggcgca gcgtatcggt
    67381 cgccacaaga cccagttcct tacatgccca tttcaccggg attggattgg gttcgacaaa
    67441 tagtttgttg tgtaatggca tcagacgctg attaataacg cgtgcctcgg caaaatgccc
    67501 ttctgctgcc agtttgcaca tctgggccat atcacgcgct gcgacgttag ccgtaacgga
    67561 aataactcca tgaccgccca attgcatgaa gtccagcgcg ctcgcatcat cgccgctcag
    67621 cagaacaaaa tcatctgaaa ccagctcttt gatctggttt acacgcgtta agttccctgt
    67681 tgcctctttg attccgataa tattttttac tttcgccaga cggcccaccg tttccgggag
    67741 cagatcgcag ccagtacggg acggcacatt atacagaatt tgcggcaggt cagtatgctc
    67801 agcgatggct ttgaaatgct gatacaaacc ttcttgcgac ggacgattgt agtaaggggt
    67861 taccgtcagg cagccgacga taccactgtc attgaagcgc tgcgtcaggc taatggcttc
    67921 cgcagtagcg ttagcgccgg tcccggcaat taccggaatg cgcccatcag ccagatccag
    67981 cgtcatcatc accacatcag catgttcgtc atgatttaag gtagcggact cgccagtggt
    68041 gccaacagaa acgatcgccg aagtaccgct ggcgacatga taatcaatca gttttttcaa
    68101 gctagcccga cagacattac ctttttcatc catcggagta acaatcgcga caatacttcc
    68161 cgtgaacatg ggccatcctc tgtgcaaaca agtgtctcaa tggtacgttt ggtatggcat
    68221 taaaagcaag cagacagaac cgttctgatt gttgtatgca tgattttttt atgctttcct
    68281 taagaacaac tcacccctta aaggaataac cagtttgaca ctgtcatcgc aacattatct
    68341 ggtgatcact gcgttgggtg ccgatcgccc tggaattgtg aacaccatca cccgtcatgt
    68401 cagtagttgc ggctgtaata ttgaagacag tcgcctggcg atgctgggag aagagttcac
    68461 gtttatcatg ctgctttccg gttcatggaa tgccatcact ctgattgaat caacgttacc
    68521 gttgaaaggt gccgaactgg atcttttaat cgtgatgaag cgcacgacgg cgcgtccgcg
    68581 tccgccaatg ccagcatctg tctgggttca ggtcgatgtt gcagactccc cgcatttaat
    68641 tgaacgcttc acagcacttt tcgacgcgca tcatatgaac attgcggagc tggtgtcgcg
    68701 cacacaacct gctgaaaatg aacgggctgc gcagttgcat attcagataa ccgcccacag
    68761 ccccgcatct gcggacgcag caaatattga gcaagcgttc aaagccctat gtacagaact
    68821 caatgcacaa ggcagtatta acgtcgtcaa ttattcccaa catgatgaac aggatggagt
    68881 taagtaatga atccactgaa agccggtgat atcgcaccga aatttagctt gccggatcaa
    68941 gacggagaac aagttaattt gaccgacttc cagggacagc gtgttctggt ttatttctac
    69001 ccgaaagcca tgacccccgg ctgtaccgta caggcctgcg gcttacgcga taacatggat
    69061 gagttgaaaa aagcgggcgt tgatgtgctg ggtatcagca ccgataaacc cgaaaaactc
    69121 tcccgttttg cggaaaaaga gctgcttaac tttacgctcc tgtctgatga ggaccaccag
    69181 gtgtgcgaac aattcggcgt ctggggtgaa aagtccttca tgggcaaaac ctacgatggt
    69241 attcatcgca tcagcttcct gattgacgct gatggcaaaa tcgaacatgt ctttgacgat
    69301 ttcaaaacca gcaatcacca cgacgttgtg ctgaactggc tgaaagaaca cgcctgatta
    69361 ctttgctcca ttccgtgctg gctgcgcttg cggccagcat acctcacttc tcgtgatcaa
    69421 gatcacattc tcgctttccc ctgcgacacg ggtgtcgaat ccattttttg ctgaacgtta
    69481 atgaccatca tttttgtacc gttcagaatc cagttaatac ataacttatt gaatagattg
    69541 agttaatcag aatggcatcc tttatacaat atgaaatgca atgtttcata tcattttcaa
    69601 ggagccgaca tgaaccgctt tgtggtggcc gaaccactgt ggtgtacagg atgtaatacc
    69661 tgtctcgctg cctgttcgga cgtgcataaa acgcaaggtt tacagcaaca cccgcgcctg
    69721 gccctggcga agacgtcaac aatcactgcc cctgtcgtgt gtcatcactg tgaggaagcc
    69781 ccttgcctgc aggtctgccc ggtcaatgcc atctctcaga gggatgatgc gatccaactc
    69841 aacgaaagcc tctgtattgg ctgcaagctt tgcgccgtgg tctgcccatt tggcgcaatc
    69901 agcgcttcag gaagccgtcc ggtgaatgcc catgcgcaat atgtttttca ggctgaaggc
    69961 tcactcaaag acggcgaaga aaacgtgccg ccccaacatg cgttgctgcg ctgggaaccg
    70021 ggtgtccaga ccgtcgcggt gaaatgcgac ctgtgtgatt tcttgccaga aggtccggcc
    70081 tgcgttcgcg cttgcccgaa tcaggcgtta cggctgatca ccgacgatag cctgcaacgt
    70141 cagatgaaag aaaaacagcg ccttgccgca agctggtttg ccaatggcgg ggagtctccc
    70201 ctttccctca ctcaggagca acactaatgg atgccctgca attattaacc tggtcgctga
    70261 tcctctatct gtttgccagt ctggcttcgc tgtttttact cggtctggac agactggcta
    70321 ttaagctttc cggcatcaca tcgctggtgg gcggcgtgat tggcatcatc agcggaatta
    70381 cgcaattaca tgctggtgta actttagtcg cccattttgc cacccctttt gactttgccg
    70441 atttaaccct gcgaatggat agcctctcgg catttatggt gctagttatc tccttgctgg
    70501 tggtggtttg ttcgctctat tcattgactt atatgcgcga atacgagggc aaaggcgcgg
    70561 cggcgatggg cttctttatg aatcttttca tcgcatcgat ggttgccctg ctggtgatgg
    70621 acaacgcttt ttggttcatc gtgctgtttg aaatgatgtc gctgtcttcc tggtttctgg
    70681 tcattgccag gcaggataaa acgtcgatca acgctggcat gctctacttt tttatcgccc
    70741 acgccggatc ggtgctgatt atgatcgcct tcttgctgat ggggcgcgaa agcggcagcc
    70801 tcgattttgc cagtttcgca cgctttcact ttctccgggg ctggcgtcgg cggtgtttct
    70861 gctggccttt ttcggttttg gcgcgaaagc cgggatgatg ccgttgcaca gctggttgcc
    70921 gcgcgctcac cctgccgcac catcgcacgc ttcggcgttg atgtctggcg taatggtcaa
    70981 aatcggtatt ttcggcatcc tgaaagtagc gatggatctg ctggcgcaaa cgggtttgcc
    71041 tctgtggtgg ggcattctgg tgatggcgat cggcgcaatc tccgcgctcc tgggcgtgct
    71101 atatgcgctg gcggaacagg atatcaaacg gctgctggcc tggagtaccg tcgaaaacgt
    71161 cggcattatt ttgctggcag tcggtgtggc gatggacggt ctgtcactgc acgacccgct
    71221 gctcaccgtg gttggactgc tcggcgcact gtttcatctg ctcaaccatg cgctgttcaa
    71281 agggctgcta tttctcggcg cggacgcgat tatttcgcgt ttgcataccc acgacatgga
    71341 aaaaatgggg gcactggcga aacggatgcc gtggacagcc gcagcatgcc tgattggttg
    71401 cctcgcgata tcagccattc ctccgctgaa tggttttatc agcgaatggt acacctggca
    71461 gtcgctgttc tcactaagtc gtgtggaagc cgtagcgcta caacttgcgg gtcctattgc
    71521 tatggtaatg ctggcagtca ctggtgggct ggcagtaatg tgcttcgtca aaatgtacgg
    71581 tattactttc tgtggtgcgc cgcgcagtac acacgctgaa gaggcacagg aagtgccaaa
    71641 tacgatgatc gtcgccatgc tactgctcgc ggcactctgc gtattaattg cgcttagtgc
    71701 cagttggctg gcaccgaaga taatgcatat tgcccatgcg tttaccaata cccctcccgt
    71761 cactgtcgcc agcggaatag cacttgtacc cggcacgttt catacacggg tcactccttc
    71821 attactgttg ctgttactac tggcgatgcc tttgctgcca ggcctttact ggctgtggtg
    71881 tcgttcgcgc cgcgcagcgt ttcgtcgcac aggagatgcc tgggcatgcg gctacggctg
    71941 ggaaaacgcg atggctccgt caggcaatgg cgtgatgcag ccgctgcgtg tggtcttctc
    72001 tgcgttattt cgtctacgac aacagctcga ccctacgctg aggctaaata aaggtcttgc
    72061 gcacgtcacc gccagggctc agagcacaga acccttctgg gatgagcggg tgatccgccc
    72121 catcgtgagc gccacccaac ggctggtcaa agaaatacag catctgcaaa gcggcgactt
    72181 tcgtctctat tgcctgtatg tggtcgccgc actggttgtg ctgctaatcg ctattgccgt
    72241 ctaaggaaat caccatgaga caaactcttt gcgacggata tctggtcatt tttgcgttag
    72301 cacaggccgt gattctgctg atgctaaccc cactttttac gggtatttcc cggcagatac
    72361 gcgcgcgtat gcactcccgc cgcgggccgg ggatctggca ggattatcgc gatatccaca
    72421 aactgtttaa acgccaggaa gttgcgccga catcttcagg tctgatgttc cgcctgatgc
    72481 cgtgggtatt aatcagcagc atgctggtgc tggcgatggc cttaccacta tttattaccg
    72541 tttccccttt tgcgggcggc ggcgatctga tcacccttat ctatcttctt gccctgtttc
    72601 gttttttctt tgctctttcc gggctggata ccggaagtcc gtttgcggga gtcggtgcca
    72661 gtcgcgagtt gacgctcggc attctggtcg aaccaatgct tattctctca ctgctggtat
    72721 tggcgctgat agcaggttcc acgcatatcg agatgatcag caatacgctg gctatgggct
    72781 ggaactcgcc gctaaccacc gtactggcgt tactggcctg tggttttgcc tgcttcattg
    72841 agatgggaaa aattcccttt gatgttgctg aagcagaaca ggaattacag gaaggcccgc
    72901 tgaccgaata ttccggtgcc gggctggcgc tagcgaaatg ggggctgggg ctgaaacagg
    72961 tcgtgatggc agcactgttt gtggccctgt ttctgccctt tgggcgcgcg caagaacttt
    73021 ctctcgcctg cctgctgact tcacttgtcg ttacgctgct caaggttttg ctgatttttg
    73081 tactggcctc aatcgcagaa aacacgctgg cacgcgggcg ttttttactc attcaccatg
    73141 tgacctggct tggcttcagc cttgctgcgc ttgcatgggt cttttggtta accggtctgt
    73201 aaggagcact gacggaatat ggaaaatctt gctctgacga cgttaatgct gccttttatc
    73261 ggcgcactgg tcgtttcgtt ttcgccacaa cgtcgggccg ccgaatgggg ggttttgttc
    73321 gccgcgctga ccacgctgtg catgttgtcg ctgatctccg cgttttatca ggccgataaa
    73381 gttgccgtca cgttgacgtt ggtcaacgtg ggggatgtgg cattatttgg cctggtcatt
    73441 gatcgcgtga gtacgctgat tctgtttgtg gtggtgtttc tcggtttgct ggtcacgatc
    73501 tactccacgg gttatctgac ggataaaaat cgcgaacacc cgcataacgg cacgaatcgt
    73561 tattacgcat ttttgctggt gtttatcggc gcgatggcgg gactggtact ctcctcgacg
    73621 ctgctcggtc agttgttgtt ttttgaaatt acgggcggct gctcctgggc gttgatcagt
    73681 tattaccaga gcgataaagc gcagcgttca gcactaaaag cgttgcttat cacccatatc
    73741 ggttcgttgg ggttgtatct tgccgccgcc acgctgtttt tgcagaccgg aacgtttgtg
    73801 cttagcgcga tgagcgagtt acatggcgac gcacgttatc tggtttatgg cggcatcctg
    73861 tttgccgcgt gggggaaatc ggcccagcta ccgatgcaag cgtggctacc ggacgcaatg
    73921 gaagcgccaa caccgatcag cgcctatctc cacgccgcat cgatggtgaa agtgggcgtt
    73981 tacatttttg cccgcgccat tatcgacggc ggcaatatcc cgcatgtgat tggcggcgtt
    74041 ggcatggtca tgacactggt caccattctt tatggctttc tgatgtattt gccacagcag
    74101 gatatgaagc ggttgctggc ctggtcgacc atcactcaac ttggctggat gttcttcggc
    74161 ttgtcgctct ccatcttcgg ctcgcggctg gcgctggagg gtagcatcgc ctacatcgtc
    74221 aaccacgcgt tcgctaaaag cctgtttttc cttgtagcag gtgcgctgag ttacagctgc
    74281 ggcacgcgct tgttgccgcg tctgcgtggc gtattgcaca ccctgccgtt gccaggcgtg
    74341 ggtttctgcg tggcagcgct ggcaattacc ggtgtgccgc cgttcaacgg cttcttcagt
    74401 aaattcccgc tgtttgccgc cggttttgcg ttgtcagtgg agtactggat cctgctgccc
    74461 gccatgattc tgctgatgat tgaatcggtc gccagtttcg cctggtttat tcgctggttt
    74521 ggtcgcgtcg tgccgggcaa accgagcgag gccgtcgccg atgccgcacc gctgccaggg
    74581 tcaatgcgcc tggtgttgat tgtactgatt gtgatgtcgc tgatttccag cgtaatcgcc
    74641 gcgacctggt tgcagtaagg agatgatgaa tgaccggttc tatgatcgta aataatctgg
    74701 cgggactgat gatgctgaca tcgctgtttg tgattagcgt caaaagctat cgcctgtcat
    74761 gcggatttta cgcctgccag tcgctggtgc tggtgtctat tttcgccact ctctcgtgcc
    74821 tgttcgccac agagcaactg ctgatctggt ccgccagcgc ctttatcacc aaagtgctgc
    74881 tggtaccgtt aatcatgact tacgctgcac gaaatattcc ccagaacatc ccggaaaaag
    74941 cgttattcgg tccggcaatg atggcactgc tcgcggcgtt aattgtcctg ctttgcgcat
    75001 ttgtcgttca gcccgtgaag ctaccgatgg ctaccgggct gaaaccggcg ctggcggtag
    75061 cgttaggtca ttttctgctt ggcctgctgt gcattgtcag ccaacgcaat atcctgcggc
    75121 aaatttttgg ttactgcctg atggaaaacg gctcccatct ggtgctggcg cttcttgcct
    75181 ggcgagcacc ggaactggtg gaaataggta tcgctaccga cgccatcttc gccgtcattg
    75241 tgatggtgtt actggcaaga aaaatatggc gtacccacgg cacgctggac gtgaacaact
    75301 tgaccgcgct gaagggataa tgagatgagt tattctgtga tgttcgcttt actcctgctc
    75361 acgccgctgc ttttttcgct gctctgtttt gcctgccgga aacgggggct ctctgcgact
    75421 tgtacggtga ctgtattaca tagcttaggg atcacactgc tgctgattct agcactctgg
    75481 gtggtccaaa ctgccgctga tgcaggagaa atattcgctg cgggactgtg gcttcatatt
    75541 gatggtctgg gcggtttgtt cctcgccatt cttggtgtga ttggctttct caccggtgtt
    75601 tactcgattg gctacatgcg tcatgaagtg gcacacggcg agctttcacc cgttacactg
    75661 tgcgattact acggtttctt ccatctgttt ttgttcacca tgctgctggt tgttaccagc
    75721 aataacctga ttgtgatgtg ggcggcgatc gaagccacca ccttaagctc ggcgtttctg
    75781 gtaggcattt acggtcagcg ttcatcgctg gaatcagcat ggaagtacat cattatttgt
    75841 actgttggtg tcgcttttgg tctgttcggt accgtgctgg tatacgccaa cgccgccagc
    75901 gttatgccgc aggcagaaat ggcgatattc tggagcgagg ttcttaagca atcgtccttg
    75961 cttgacccaa cattaatgct gttggccttt gtgtttgtgc taattggctt tggtaccaaa
    76021 accgggctat ttcccatgca cgcctggctg ccggatgctc acagtgaagc gccgagtccg
    76081 gtcagcgccc tgctctccgc cgtattgctg aactgcgcgc tgttggtgct gattcgctat
    76141 tacatcatta tttgccaggc catcggcagc gatttcccca accggttgtt gctcatcttc
    76201 ggcatgttgt cggttgccgt ggcggcattt ttcattctgg tacagcggga cattaagcgt
    76261 ctgctggcgt actccagcgt ggagaacatg gggctggtcg cggtggcgct aggcattggc
    76321 gggccgctgg gaatttttgc cgcgctgctg cacaccttaa accacagtct ggcaaaaacg
    76381 ctgctgttct gcggttccgg caatgtactg ctcaagtacg gcacgcgcga tctcaacgtc
    76441 gtctgtggga tgctcaaaat catgccattt accgccgtgc tgtttggcgg cggtgcgctg
    76501 gcgctggcgc tggcagggat gccgcccttc aacatttttc ttagcgaatt tatgaccgtt
    76561 accgcaggac tggcacgtaa tcacctgctg attatcgtcc tgctgttatt gctgttaacg
    76621 ctggtgctgg cgggcctggt acggatggct gcgcgggtgt taatggcgaa accgccgcag
    76681 gccgttaacc ggggtgatct cggctggttg accacctcgc caatggtgat tctgctggtc
    76741 atgatgctgg cgatgggaac gcatattcca caacctgtca tcaggatcct ggcgggcgct
    76801 tccactatag tcctctcagg gacgcacgac ctgcctgcac aacgtagcac ctggcatgat
    76861 tttttgcctt caggcaccgc atctgtttcg gagaaacaca gtgaacgtta attcatcgtc
    76921 aaatcgtggc gaagcgattc tcgccgccct gaaaacgcag ttccccggcg cggtgctgga
    76981 tgaagagcga caaacgcctg aacaggtcac cattacggtg aaaatcaatc tgctgcctga
    77041 cgttgtacat tatctttatt atcaacatga tggctggctt ccagtcctgt ttggcaacga
    77101 cgagcggaca cttaacggtc attacgcggt ttattatgcc ctttcaatgg aaggggccga
    77161 aaaatgctgg atcgtggtga aggcactggt cgatgccgac agtcgggagt ttccgtcagt
    77221 cacaccgcgc gtccctgccg cggtctgggg cgagcgagaa attcgcgata tgtacgggct
    77281 gattccggtt ggcctgccgg atcagcgtcg cctggtgttg cccgatgact ggccggaaga
    77341 tatgcatccg ctgcgcaaag atgcgatgga ttatcgactg cgccctgaac cgacgacaga
    77401 ttccgaaacg tatgcgttta tcaatgaggg caacagcgat gcgcgggtga tccctgtcgg
    77461 cccgctgcat atcacctcct atgaaccggg tcacttccgc ttgtttgtgg atggcgagca
    77521 aattgtcgat gctgattacc gcctgtttta tgtccatcgc ggcatggaga aactggcaga
    77581 aacgcggatg ggctacaacg aagtgacctt cttatcggac cgcgtgtgtg ggatttgcgg
    77641 ttttgcccac agtgtggcct ataccaactc ggttgaaaat gcactgggga ttgaggtgcc
    77701 gcaacgagca catactattc gctcgattct gctggaagtc gagcggctac acagtcattt
    77761 gctcaacctt ggcctctcct gccattttgt tggttttgat accggcttta tgcaattttt
    77821 ccgcgtgcgg gaaaagtcga tgacgatggc ggaattgctg accgggtcgc gtaaaaccta
    77881 cggtctgaat ctgattggtg gtgttcgccg cgatattctc aaagagcaac gtctgcaaac
    77941 gctgaaactg gtgcgcgaga tgcgcgccga cgtgtcggag ctggtagaga tgctgcttgc
    78001 tacgccgaat atggaacaac gcactcaggg cattggcatt ctcgaccgac aaatcgcccg
    78061 tgattatagc cctgtagggc cgctgatccg cgcagtggtt ttgcccgtga tttgcgcttt
    78121 gatcacccct acgccgacta cggcaatatt ccaaaaacac tgtttacctt taccggcggc
    78181 gatgttttct cccgcgtgat ggtccgtgtc aaagagacgt ttgattcgct ggcaatgctg
    78241 gaatttgccc tcgacaacat gccggatacc ccactgctga ccgaaggctt tagctataaa
    78301 cctcacgcat tcgcgctggg ctttgttgaa gcgccacgcg gtgaagacgt gcactggagc
    78361 atgctcggtg ataaccaaaa attgttccgc tggcgctgtc gtgccgccac ctacgccaac
    78421 tggccggtgt tgcgttacat gctgcgcggc aataccgttt ctgacgcacc gctgattatc
    78481 ggtagccttg atccctgcta ctcctgtacc gaccgtgtga cgctggtaga tgtgcgcaag
    78541 cgccagtcaa aaaccgtgct gtataaagag atcgaacgct acggcattga tcgtaaccgt
    78601 tcgccgctga agtaaggaca gaagatgctg aagttactga aaactattat gcgcgccgga
    78661 accgcgacgg tgaaatatcc cttcgcgcca ctggaggtca gcccaggctt tcgcgggaaa
    78721 ccggacctga tgcccagcca atgtattgcc tgcggtgcct gcgcctgtgc ttgtccggca
    78781 aatgcgctga ctatccagac cgacgaccag caaaattcgc gcacctggca gctctatctg
    78841 gggcgttgta tttactgcgg acgttgtgaa gaagtgtgcc cgaccagagc catccagctt
    78901 accaataact ttgaactgac cgtcacccat aaagccgatc tctataccca cgcgacgttc
    78961 catctacaac gttgcagccg ttgcgaacgc ccgtttgccc cgcaaaaaac cgtcgcactg
    79021 gctgctgaat tgttggcaca gcaacaaaat gctccacaaa accgcgaaat gttatgggcg
    79081 caagcgagcg tctgcccgga gtgcaaacaa cgcgcaacgc tgatcaacga cgatacagat
    79141 gtaccgctgg tggctaagga gcagctatga gtccagtgct tacgcaatat gtcagccagc
    79201 ccatcacgct ggacgagcaa acgcaaaaga tgaagcggca tttgctacag gatatccgtc
    79261 gctcggctta cgtttatcgc atcgattgcg gcggctgcaa cgcctgtgaa atcgaaattt
    79321 ttgctgccat cacccccgta ttcgacgcag aacgttttgg cattaaggtt gtttcatcac
    79381 cgcgtcacgc cgatattttg ttatttactg gcgcagtcac ccgggcgatg cgtatgcctg
    79441 cacttcgggc gtatgagtct gcccccgatc ataaaatctg tgtttcctac ggcgcgtgcg
    79501 gtgtcggcgg cggtattttc cacgatctct acagcgtctg gggcggcagc gacaccattg
    79561 tccccattga tgtttggatc cccggctgcc cgccaacacc ggccgccacc attcacggtt
    79621 tcgccgtggc gctcggtttg ctgcaacaga agattcacgc ggtggattat cgcgatccca
    79681 ccggggtgac tatgcaaccg ttgtggccgc agatcccgcc atcacagcgt atcgccattg
    79741 agcgagaagc gcggcggctg gcgggctatc gtcaggggcg agaaatttgc gatcggctcc
    79801 tgcgccattt aagcgacgat cctacaggaa atcgggttaa cacctggttg cgcgatgccg
    79861 acgatccacg tctcaatagt atcgttcagc aactctttcg cgtactccgg gggttacatg
    79921 actgaagagt gcggggaaat tgttttctgg acgctgcgaa aaaagtttgt cgccagtagc
    79981 gacgagatgc cggaacacag ctctcaggta atgtattact cgctggctat cggtcatcac
    80041 gttggcgtga ttgattgtct gaatgtcgcc ttccgctgcc cactgacgga atacgaagat
    80101 tggcttgcac tggtcgaaga ggagcaagcc cgacgtaaga tgctgggggt gatgactttt
    80161 ggtgagattg ttattgacgc cagccacacc gccctgttga cccgggcatt cgcgccactg
    80221 gcagatgacg cgacgtctgt gtggcaagcg cgtagcattc aattcattca tctgttggat
    80281 gaaattgtgc tggaaccggc tatctatctg atggccagaa aaattgcgtg agaaggattt
    80341 ctcattaata aggactgttg atggctatgt cagacgaagc gatgtttgcc ccgccgcaag
    80401 gaataacaat tgaagcggta aacggaatgc tcgcggagcg gttagcgcag aaacacggca
    80461 aggcgtcttt attacgcgcc ttcatcccgc tgccgccgcc gttcagcccg gtacaactta
    80521 ttgaactgca tgttctcaaa agcaacttct attaccgcta ccatgatgat ggcagcgatg
    80581 tgacggcaac aacagagtat cagggcgaga tggtcgatta ttcgcgttac gccgtccttc
    80641 tcggcagtag tggaatggcg gagctacgct ttattcgtac ccacggcagc cgttttactc
    80701 cccaggattg cacactgttt aactggctgg cacgcattat caccccggtt ctgcaatcat
    80761 ggctcaatga tgaagcacag caggtggcgc tgcgtttgct ggagaaagat cgcgatcatc
    80821 atcgggtact ggtggatatc actaatgcag tgctgtcaca tcttgatctc gacgatctga
    80881 tcgctgacgt cgctcgtgag atccatcatt ttttcggtct ggcttcagtc agtatggtac
    80941 tgggcgatca tcgaaagaac gagaagttca gcctgtggtg cagcgatctt tctgcctcac
    81001 attgtgcgtg tctgccacac aatatgcctg gcgacagtgt attgctgaca caaacgctac
    81061 aaacccgaca accgaccttg acgcaccgtg cagatgatct gtttctctgg caacgcgacc
    81121 cgttattact cttacttgca tctaacggct gcgaatctgc gctccttata ccgcttacct
    81181 ttggcaacca tacaccgggt gcattgttgc tggcgcatac ctcttccact ctctttagtg
    81241 aggaaaactg ccagctacta caacacatag ccgatcgcat cgctattgcc gttggcaatg
    81301 ccgatgcctg ccgtagaatg accgatttgc aggaaagttt gcagcaagaa aaccaccagc
    81361 ttagcgagca gctcctttcg aatctgggcg tcggtgacat tatctatcaa agccaggcaa
    81421 tggaagacct gctccagcag gtagatattg tggcgaagag cgacagtacg gtgttgattt
    81481 gcggtgaaac cggaaccggc aaagaggtga tcgccagagc gatccatcaa cttagcccgc
    81541 gacacgacaa gccgctggtc aaaatcaact gcgctgccat ccccgccagt cttctggaaa
    81601 gtgagttatt cggtcatgac aaaggggcgt ttactggtgc gattaatacc catcgtggtc
    81661 gttttgaaat tgccgatggc ggcacgttgt ttctcgatga aattggcgat ctgccgttag
    81721 aacttcagcc taaactgctg cgcgtattgc aggaacggga gattgagcgt ctcggcggga
    81781 gtagaacgat cccggtaaat gtcagagtca ttgccgccac caaccgtgat ttgtggcaaa
    81841 tggttgaaga tcgccagttt cgcagcgatc tcttttatcg cctgaatgtc ttcccactgg
    81901 aattgcctcc gctgcgcgac cgtccggaag atatccctct tttagcaaag catttcacgc
    81961 aaaaaatggc gcgccatatg aatcgttcaa ttgacgccat cccgaccgag gcgctacgcc
    82021 agttgatgtc gtgggactgg ccgggcaacg tgcgcgagct ggaaaacgtg attgagcgag
    82081 cggtactgct gactcgcggt aacagtctga atttacatct taatgtccga caaagccgtt
    82141 tactgccgac gctaaatgaa gattcagcgc ttcgcagttc aatggcgtag ttgctgcacc
    82201 cgacgacgcc agagaatgac gaagaagaac gtcagcgcat tgttcaggta ttgcgagaaa
    82261 ccaatggcat tgttgccggg ccccgtggcg cggcgacacg attagggatg aagcgcacca
    82321 cgctgctgtc acgaatgcag cgtctgggaa tctcggttcg cgaggtgttg taatctgctt
    82381 ttgcaggagt atgcatgaga aacaaactct ctttcgactt gcagttgagc gccagaaaag
    82441 cggcaatcgc tgaacggatt gccgcccata aaattgcccg cagtaaagtg tcggtctttt
    82501 taatggcgat gtccgctggc gtgtttatgg cgatgtccgc tggcgtgttt atggcgatcg
    82561 gatttacttt ttacctttcc gttatcgccg atgccccgtc ttcataggca ttaacccatc
    82621 tggtgggcgg cctttgcttt acacttggct ttattttgct ggcggtttgc ggcaccagcc
    82681 tgttcacctc gtcggtaatg acggtaatgg caaaaagtcg gggcgttatt agttggcgaa
    82741 cttggctgat taacgcactt ctggtggcct gcggtaatct ggcaggtatt gcctgtttca
    82801 gtttgttaat ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg ggagtcgcga
    82861 ttttacactg cgccgagggc aaaatgcatc atacatttac tgaatctgtc agcctcggca
    82921 ttatgtgcaa tctgatggtt tgcctggcgc tgtggatgag ttattgcgga cgttcgttat
    82981 gcgacaaaat cgtcgccatg attttgtcca tcaccctgtt tgtcgccagt ggctttgagc
    83041 actgtatcgc caatttgttt gtgattccgt tcgccattgc cattcgccat ttcgccccta
    83101 cctccttctg gcaactggcg cacagtagcg cagacaattt tccggcactg acggtcagcc
    83161 attttattac cgccaatctg ctcccggtga tgctgggtaa tattatcggc ggtgcagtgc
    83221 tggtgagtat ttgtt
//
LOCUS       DAJTYH010000003        71090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTYH010000003 DAJTYH010000000
VERSION     DAJTYH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(750..2336)
                     /locus_tag="OO609_000192"
     CDS             complement(750..2336)
                     /locus_tag="OO609_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6631744.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="OO609_000193"
     CDS             complement(2691..4862)
                     /gene="glcB"
                     /locus_tag="OO609_000193"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6631745.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4884..5288)
                     /locus_tag="OO609_000194"
     CDS             complement(4884..5288)
                     /locus_tag="OO609_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6631746.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRATKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="OO609_000195"
                     /pseudo
     CDS             complement(5293..6512)
                     /gene="glcF"
                     /locus_tag="OO609_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="OO609_000196"
     CDS             complement(6523..7575)
                     /gene="glcE"
                     /locus_tag="OO609_000196"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6631747.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="OO609_000197"
                     /pseudo
     CDS             complement(7575..9074)
                     /gene="glcD"
                     /locus_tag="OO609_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9325..10095
                     /gene="glcC"
                     /locus_tag="OO609_000198"
     CDS             9325..10095
                     /gene="glcC"
                     /locus_tag="OO609_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6631748.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10219..11360)
                     /locus_tag="OO609_000199"
                     /pseudo
     CDS             complement(10219..11360)
                     /locus_tag="OO609_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11722..13452
                     /locus_tag="OO609_000200"
     CDS             11722..13452
                     /locus_tag="OO609_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6631749.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13449..14363
                     /locus_tag="OO609_000201"
     CDS             13449..14363
                     /locus_tag="OO609_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6631750.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14396..14644
                     /locus_tag="OO609_000202"
     CDS             14396..14644
                     /locus_tag="OO609_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6631751.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14644..15815
                     /locus_tag="OO609_000203"
                     /pseudo
     CDS             14644..15815
                     /locus_tag="OO609_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="OO609_000204"
     CDS             complement(15851..16930)
                     /gene="lptG"
                     /locus_tag="OO609_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6631752.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="OO609_000205"
     CDS             complement(16927..17997)
                     /gene="lptF"
                     /locus_tag="OO609_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6631753.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18028..18588)
                     /locus_tag="OO609_000206"
     CDS             complement(18028..18588)
                     /locus_tag="OO609_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6631754.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18600..19424)
                     /locus_tag="OO609_000207"
     CDS             complement(18600..19424)
                     /locus_tag="OO609_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631755.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19424..20773)
                     /locus_tag="OO609_000208"
     CDS             complement(19424..20773)
                     /locus_tag="OO609_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6631756.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20819..21577)
                     /locus_tag="OO609_000209"
     CDS             complement(20819..21577)
                     /locus_tag="OO609_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631757.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21609..22322)
                     /locus_tag="OO609_000210"
     CDS             complement(21609..22322)
                     /locus_tag="OO609_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6631758.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22496..23188
                     /locus_tag="OO609_000211"
     CDS             22496..23188
                     /locus_tag="OO609_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6631759.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="OO609_000212"
     CDS             complement(23237..24736)
                     /gene="pitB"
                     /locus_tag="OO609_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6631760.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25028..26887)
                     /gene="gss"
                     /locus_tag="OO609_000213"
     CDS             complement(25028..26887)
                     /gene="gss"
                     /locus_tag="OO609_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6631761.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27092..27958
                     /gene="yghU"
                     /locus_tag="OO609_000214"
     CDS             27092..27958
                     /gene="yghU"
                     /locus_tag="OO609_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6631762.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="OO609_000215"
     CDS             complement(28081..28329)
                     /gene="hybG"
                     /locus_tag="OO609_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6631763.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="OO609_000216"
     CDS             complement(28463..28804)
                     /gene="hypA"
                     /locus_tag="OO609_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6631764.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="OO609_000217"
     CDS             complement(28797..29285)
                     /gene="hybE"
                     /locus_tag="OO609_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6631765.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29278..29772)
                     /locus_tag="OO609_000218"
     CDS             complement(29278..29772)
                     /locus_tag="OO609_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6631766.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="OO609_000219"
     CDS             complement(29772..31475)
                     /gene="hybC"
                     /locus_tag="OO609_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6631767.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="OO609_000220"
     CDS             complement(31472..32650)
                     /gene="hybB"
                     /locus_tag="OO609_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6631768.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="OO609_000221"
     CDS             complement(32640..33626)
                     /gene="hybA"
                     /locus_tag="OO609_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6631769.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="OO609_000222"
     CDS             complement(33629..34747)
                     /gene="hybO"
                     /locus_tag="OO609_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6631770.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(34936..35223)
                     /locus_tag="OO609_000223"
     CDS             complement(34936..35223)
                     /locus_tag="OO609_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6631771.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35342..36229)
                     /locus_tag="OO609_000224"
     CDS             complement(35342..36229)
                     /locus_tag="OO609_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6631772.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36386..37426
                     /gene="gpr"
                     /locus_tag="OO609_000225"
     CDS             36386..37426
                     /gene="gpr"
                     /locus_tag="OO609_000225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6631773.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37466..37960)
                     /locus_tag="OO609_000226"
     CDS             complement(37466..37960)
                     /locus_tag="OO609_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6631774.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38151..39035
                     /gene="yghA"
                     /locus_tag="OO609_000227"
     CDS             38151..39035
                     /gene="yghA"
                     /locus_tag="OO609_000227"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6631775.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="OO609_000228"
     CDS             complement(39307..39732)
                     /gene="exbD"
                     /locus_tag="OO609_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6631776.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="OO609_000229"
     CDS             complement(39739..40473)
                     /gene="exbB"
                     /locus_tag="OO609_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6631777.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40725..41912
                     /gene="metC"
                     /locus_tag="OO609_000230"
     CDS             40725..41912
                     /gene="metC"
                     /locus_tag="OO609_000230"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6631778.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42052..42711
                     /locus_tag="OO609_000231"
     CDS             42052..42711
                     /locus_tag="OO609_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6631779.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42751..43650)
                     /gene="yqhC"
                     /locus_tag="OO609_000232"
     CDS             complement(42751..43650)
                     /gene="yqhC"
                     /locus_tag="OO609_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6631780.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43844..45007
                     /gene="yqhD"
                     /locus_tag="OO609_000233"
     CDS             43844..45007
                     /gene="yqhD"
                     /locus_tag="OO609_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6631781.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45112..45939
                     /gene="dkgA"
                     /locus_tag="OO609_000234"
     CDS             45112..45939
                     /gene="dkgA"
                     /locus_tag="OO609_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6631782.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46139..47065
                     /locus_tag="OO609_000235"
     CDS             46139..47065
                     /locus_tag="OO609_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6631783.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47116..47373
                     /gene="yqhH"
                     /locus_tag="OO609_000236"
     CDS             47116..47373
                     /gene="yqhH"
                     /locus_tag="OO609_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6631784.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47416..49635)
                     /locus_tag="OO609_000237"
     CDS             complement(47416..49635)
                     /locus_tag="OO609_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6631785.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="OO609_000238"
     CDS             complement(49746..51158)
                     /gene="ftsP"
                     /locus_tag="OO609_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6631786.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="OO609_000239"
                     /gene_synonym="parF"
     CDS             complement(51233..51970)
                     /gene="plsC"
                     /locus_tag="OO609_000239"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6631787.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52204..54462)
                     /gene="parC"
                     /locus_tag="OO609_000240"
     CDS             complement(52204..54462)
                     /gene="parC"
                     /locus_tag="OO609_000240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6631788.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55008..55490)
                     /locus_tag="OO609_000241"
     CDS             complement(55008..55490)
                     /locus_tag="OO609_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6631789.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="OO609_000242"
     CDS             complement(55543..55935)
                     /gene="ygiW"
                     /locus_tag="OO609_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6631790.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56087..56746
                     /gene="qseB"
                     /locus_tag="OO609_000243"
     CDS             56087..56746
                     /gene="qseB"
                     /locus_tag="OO609_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6631791.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56743..58017
                     /gene="qseC"
                     /locus_tag="OO609_000244"
     CDS             56743..58017
                     /gene="qseC"
                     /locus_tag="OO609_000244"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6631792.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58063..58395)
                     /locus_tag="OO609_000245"
     CDS             complement(58063..58395)
                     /locus_tag="OO609_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6631793.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58714..59295
                     /gene="mdaB"
                     /locus_tag="OO609_000246"
     CDS             58714..59295
                     /gene="mdaB"
                     /locus_tag="OO609_000246"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6631794.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59326..59640
                     /locus_tag="OO609_000247"
     CDS             59326..59640
                     /locus_tag="OO609_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6631795.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59688..61580)
                     /gene="parE"
                     /locus_tag="OO609_000248"
     CDS             complement(59688..61580)
                     /gene="parE"
                     /locus_tag="OO609_000248"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6631796.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="OO609_000249"
     CDS             complement(61609..62190)
                     /gene="yqiA"
                     /locus_tag="OO609_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6631797.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="OO609_000250"
     CDS             complement(62190..63017)
                     /gene="cpdA"
                     /locus_tag="OO609_000250"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6631798.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63042..63464)
                     /locus_tag="OO609_000251"
     CDS             complement(63042..63464)
                     /locus_tag="OO609_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6631799.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="OO609_000252"
     CDS             complement(63465..64094)
                     /gene="nudF"
                     /locus_tag="OO609_000252"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6631800.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64299..65780
                     /gene="tolC"
                     /locus_tag="OO609_000253"
     CDS             64299..65780
                     /gene="tolC"
                     /locus_tag="OO609_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6631801.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            65928..66599
                     /locus_tag="OO609_000254"
     CDS             65928..66599
                     /locus_tag="OO609_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6631802.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVLMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66605..67765
                     /locus_tag="OO609_000255"
     CDS             66605..67765
                     /locus_tag="OO609_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6631803.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67803..68591)
                     /gene="ygiD"
                     /locus_tag="OO609_000256"
     CDS             complement(67803..68591)
                     /gene="ygiD"
                     /locus_tag="OO609_000256"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6631804.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68734..69507
                     /gene="zupT"
                     /locus_tag="OO609_000257"
     CDS             68734..69507
                     /gene="zupT"
                     /locus_tag="OO609_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6631805.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69565..69735)
                     /locus_tag="OO609_000258"
     CDS             complement(69565..69735)
                     /locus_tag="OO609_000258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631806.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="OO609_000259"
     CDS             complement(69996..70649)
                     /gene="ribB"
                     /locus_tag="OO609_000259"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6631807.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70754..70902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT        17710 a        18971 c        17580 g        16829 t
ORIGIN      
        1 tcatttattc acacctaata aaagtattca aattcagatg atttttctgc tcaacagaca
       61 caaacaggca ataatactct ccaaagcgaa caatatttgg cattacgatt taatgcttca
      121 taatcaccaa attatttaac cataaatttt cttatttcta acaaaatgat tggtgtgttg
      181 ttggagttaa aatacataca taatattaac atctgccaac atttcacttt ccgcaagacg
      241 gatgatttcg aatcctcatc cacgttaccc tatcccaaat atctttatta ataggcagta
      301 acgaacactg ttatagcaag attaacttat taacctaacg gcatctttta tgtaacaagt
      361 ggagataaat tgcttaaaat tgatcctcga caaatcttaa aagttctcat tattctgcaa
      421 aatcagcttt gcgaataatc ttatgttttt gggagttttg ggaattttga tctactcatt
      481 gttctagtga aaaatataaa taagagaaaa aacgccattc gtcatgaaag caacagatga
      541 agcaatgtaa taacatacta aaattgatct catgaaagtg ggtactttat aaccagcaca
      601 gaagccaagt tgtataaata ttaaacaatt cgcatcgtaa aacggagact acgtataaag
      661 aagccaatcg taaaaacggt tccctggcga gataaacgac agacaaatgg aaagccgggt
      721 gatatttacc ttcatccggc tttattgatt tacgagacta acatcccggt aaacacatac
      781 gcctgcagca gggtgataac gctggcaaaa atcagactgt gcttcacggt gtagcggaac
      841 agttcagatt ctcggcccac catgcccgtc gcggcgcagg ccacggcgat agattgcggg
      901 gagatcatct tgccagttac gccgccgctg gtatttgctg ccaccagcag agtgtcagag
      961 acgttgattt gctgcgccgt ggtcgattgc agtgaaccaa acagggcgtt tgaagaagtg
     1021 tccgacccgg taaggaatac gcccagccag ccgagaaacg gtgagaaaaa cgggaacatc
     1081 acgcctgtac ctgccagtac cagcgccagc gtggtggaca tgccggaata gttggtgacg
     1141 aaggcgaatg ccagcaccat gccaatcgac aatatcggcc acttcagact aattagcatt
     1201 tcggcaaaga cgccaatccc tttcttgatg cccacgccga ggatgaagat agagataatc
     1261 gccgcaataa aaatagcggt gccgccagcc gagagagggt cgaatttaaa caccgcatcc
     1321 attggcgttg gttgggcgac aatgggtgcc gctttcaaca cttgttgatg caaatgaggg
     1381 atctggaaat taatcaccag tgaataaaac gcgccgcccg gagcaaataa cgctttaaac
     1441 ggcttcatgg tccagatggt gaccagcacc gttaagatta aaaacggtga ccacgcacga
     1501 atgatttgcc ccagactata ttctgaaggc acgggaccgc cagaagatgg cttatttacc
     1561 accatcgcac ctgcggattg ccccatgctg attgccgttt cggtattttt cggccgccag
     1621 actttaagga ataaagcgag tgagacgata ctcaccagcg ccgaagtaat atccggcagt
     1681 tccggaccaa tatagttaga ggtaaagaac tgagtgacag cgaagcttcc cccagcaacc
     1741 agcgccgctg gccacgtctc tttcacccct ttccagccgt ccatcattgc taccagccag
     1801 aacggcacaa gaaccgacag gaacggtaac tgacgtcccg ccattgcgcc aatgtggaac
     1861 ggatcgattc ccgttacctg accggcgacc agaatcggca cgcccaacgc accaaacgcc
     1921 accgtcgcac caaagccagc agctccttcc agcaacgcac caaaggagaa accaatcagt
     1981 aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat aatatcgaac
     2041 tgcccgctgg caacggttaa tttatacagg aacaccgccg cgacaataat ccacgcaatt
     2101 ggccataatc cataaataaa gccatagccc gcagcagcaa atgccatatc aatcggcatt
     2161 ttaaaggcga atatcgcaat caggatagat aatataaggg ttattgctcc agcaacatgt
     2221 cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat cagggcgacc
     2281 agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt aaccatattg
     2341 ttcagtctct tattatcttt atatgcttga tatacttaag gttgtaataa gcaaaagagg
     2401 actgaactgt aaaatatagg cgttatactt tacagcaaca gtacgccgct aacgcaattg
     2461 ctacctctgg cataacaagt atatcgggta agggtttctg ttccgcacac gcaggcgcag
     2521 agtatcgtta agatgtccat attgttgttt taggcccgct agtaatgcgc tacgggtatt
     2581 taatattgtt aaaccctgat aatcgctccg gttatttccg ggataaatgt actaccgcag
     2641 ttactatcat agccccgaca ataaaacttg ccggggcttt tttgacgcta ttaatgactt
     2701 tctttttcgc gtaaacgcca ggcgtgtaat aacggttcgg tataaccgtt tggctgtttc
     2761 acgccgagga agattaaatc gctggcagct ttaaaagcac acgagttagc gaaattcccc
     2821 gccatcggac gataagccgg atcgccagca ttttgctgat caaccacttt cgccatattc
     2881 tccagcgacg cctgcacctg ttctttggtc agaataccgt gacgtaacca gttggcgata
     2941 tgctggctgg agatacgcag cgttgcgcgg tcttccatca gcgccacgtt gtgaatatcc
     3001 ggcacttttg aacaaccaat cccctgctcc acccaacgca ccacgtaccc cagaatcccc
     3061 tgcacgttgt tatccagctc ttgttggatc tcttgcgccg accagttagc gttttcagcc
     3121 accggaatag tcagcagatc gtccagcagc ggttcaaatt cagcactgaa ctcggtctgg
     3181 gcaatgttgg cttgcacgct ctgtacgttg gtatggtggt agtgcagcgc atggagcgta
     3241 gcagcggttg gtgacggaac ccaggctgtg tttgccccgg cacgcagttg gtcgcccttc
     3301 tggctgtaca tgtctgccat caggtccggc attgcccaca tgcctttacc gatttgcgct
     3361 ttaccgcgca gcccacagaa cagaccggaa agcacgttat tacgttcgta ggctttgatc
     3421 cacggcgtcg atttcatctg gtttttacgc agcatcgggc cagcttccat caccgaatgc
     3481 atttcatcgc cggtacggtc gaggaaaccg gtattgatga acgccacgcg gttgcgcgcc
     3541 tgagcgatac agctacgcaa gttcagcgag gtccggcgtt cttcatccat aatgcccatt
     3601 ttcagggtat tcggtgccat accgagcatt gtctcaatgc gggtaaacag tttgttggcg
     3661 aacgccactt cctgcggacc gtgcattttc ggtttcacaa tatagacgct gccagtgcgc
     3721 gagtttttct gcacttttaa atcatagagg gcaatcgcgc cagtcatgac gccatcaaga
     3781 ataccttccg ggatttcatt gccttcgctg tcccaaatca caggaatggt catcaaatga
     3841 cccacgttgc ggataaacag caacgagcgt ccgtgcagag aaatttcaga gccatcggcg
     3901 gcggtgtact gacgatcgtc attcagttta cgtacgattt gccgaccgtt tttctccatt
     3961 ttctcttgca gagtcccctg catcaggccc agcagattgc ggtacagcag gattttatct
     4021 tccgcatcaa ccgccgcgac cgaatcttcg cagtcgagaa tggtactgat agcagcttcg
     4081 acgataacat cgttgatgtg cgccggatcg tctttgccaa tccgcccgtt ggcatcaatt
     4141 tgcagttcaa tatgcaggcc gttatttttc agcaaaatgc aggtcggcgc agcggcatcg
     4201 ccacggtaac cgacaaactg tgctggagta cgtaacgtgg tttctttacc atttttcaac
     4261 tggatgcgta attgtttatc gaccacctta aacgccacca catcctgata gctgccgttt
     4321 tccagcggta gtgattcatc gaggaaacgt cgaacccagg cgataacctg ctcaccgcgc
     4381 tgcggatcgt agccactgac catcgcccct tcctgcggga tgatgtcgct gccgtataac
     4441 gcatcgtaca gtgagcccca gcgagcgttc gccgcgttca gcgcgtagcg ggcgttcatt
     4501 gccggaacca ccagctgtgg ccccgcctga ctggtgattt cgctgtcaat gcccgtggtt
     4561 tccaccgtca cgcgctccgg ttgcggcacc aggtagccca gttcacgaag gaaagattta
     4621 taggccgctt tatcttttac cggccccgga ttgctgcgat gccactcatc aagcgccgcc
     4681 tgaatgcgat cgcgttctgc cagcaactga cgattttctg gtgccagatc gtgaacgatc
     4741 tcatcaacat tgtgccagaa tgcagcagcg tccagttcca cgcccggcaa aacttctcca
     4801 tccacaaaac gtttaaaatt ggcgtcaatg cgtaaacggc cctgggttat ggtttgactc
     4861 attgtttctc tcctcgtttt cgcttatttc gccaacaccg ctgctgccac tttcgcgacc
     4921 tgcgcatcct gtgctccggt taaaccagaa acgcccacgg caccaataat ttgcccatca
     4981 acaacaaccg gtacgccgcc ttccagcgac gtcagtaacg gcgcagtcac gaacgcggta
     5041 cgtccgttgt tcaccatctc ttcatagccc ttagttgcac gacgccccag cgcggcggta
     5101 cgcgctttct cctgggagat gtaagccgca atcggcgcgc aatcgtccat gcgacttaac
     5161 gccagcagat gaccgccgtc atcggcaaca gcaatggaaa cagaccagtt atttttctgc
     5221 gcctcttcct gacctgcggc aataattgca ctcgccattt gctggctaag aatgacttta
     5281 gttttcattt ttttattcct tttcaagggc ttgttctaca atttcaatcc agtgacgcac
     5341 agaggtacga ccggcgctcg ccagatgcgt ctggcaacca atgttggcgg tgacgatcat
     5401 ttccggtttg ccgctttcca gcgcattcat tttgttatcc cgcagctggc gtgccagatc
     5461 gggatgcgtt aacgcatatg ttcccgctga accgcagcac agatggctgt cgggaacgtc
     5521 cgttaaggta aatccaagac gaagtaacac tttttccact tcgccgttca gcttttgcgc
     5581 atgttgtagg gtacacgggc agtggaaggc cagtttttta tcgccgcgaa ttgccagttt
     5641 ttccagcggt tcctcgcgca gaagttcgac taaatcgacc gccagttcac tgacctgacg
     5701 cgctttatcg gcatataacg catcgttttt cagcatctgc ccatactctt tgacgaacgc
     5761 gccgcaaccg ctggcggttt gcaaaattgc ctcggcacct gcttcaatcg cgggccacca
     5821 ggcatcaata ttattgcgcg cccgtgccaa ccctttctcc tgcgcattaa gatgatagtc
     5881 caccgcgcca caacagcctg cttcgttagc tgacatgacg ctgatcccca gacgatccag
     5941 cactcgcgca gttgccgcgt tggtgttggg cgaaagcgta ggctgggcgc agccttccag
     6001 cattaaaacc cgacgcttat ggcgcagcgg cggacgcggt ttagctttca ccgtttcagc
     6061 aggcagtttt gctctgacct gttccggtaa aaacggtcgc agcaccagcc ctacctgcgt
     6121 cagcgcacgg aagaccgccg gacgcggcac tacctggcgc aatccttcgc gcagcattcg
     6181 ctccggcagt gggcgtttca ctttctgctc gacaatatca cgcccgatat ccagcaaatt
     6241 gtgatagcgc acaccagaag gacaggtggt ttcacaattg cggcaagtga ggcagcgatc
     6301 gagatgctcc tgtgttttaa gcgtgacttc gttgccttcc agcacctgtt taatcagata
     6361 gatgcgcccg cgcggcccgt ccagttcatc gcccagaagc tgataggttg ggcaggttgc
     6421 ggtacaaaat ccgcagtgaa cacaggcgca caggatgctg tcggcttcca gcgcgcgttc
     6481 tgccgcatct cttcggttaa tcgggtttgc atagcctgct cctcaaagtt ccgcgtacat
     6541 gcgaccgggg ttaaacacgc cgcaagggtc gagctgctgt ttaagctgct ggtgatagcg
     6601 gaataaagga gccgatagcg gggcaaagcc accatctccg gcactaaagc gggtcgcatg
     6661 accgccagcg ttgcgggcga tgcgatggat ttgattgtca tcggctgtcg atttcagcca
     6721 gcgtaacgcc ccgccccagt cgatcagttg ctcgccgggt aaatccatca tcggcgcatc
     6781 gctgggtaat gaaatgcgcc ataaggtacc tggtaacgag aagaacggca gttgttgttc
     6841 acgcaattgc tgccagaact gacccacaac ctcttcgcca cccagcagtt cacgcgctgc
     6901 ttttaccgat ccttcgccgc cctcaaggcg gatccacaac gcattgtcga agtaacataa
     6961 gccactaatg ggtaatggct ggagttgcca ctcggcgatt tcactcatgg cttcttgcag
     7021 gctgatttcc cgacgcaggc tcagagaggc acgcggtcgc ggtaacactt tcattgagat
     7081 ttcagtgagc acgccaagac aaccgtagct tccggccatt aaccgtgaga gatcgtatcc
     7141 ggcaacgttt ttcatcactt cgccaccaaa acgcagatgt tttccagtgc cggtaatgat
     7201 gcgcgtgccg aggacaaaat cgcggaccga accgctccac gggcgacgcg gccccgccag
     7261 cccgcaggcg accatcccgc cccaggtggc ttcttcacca taatgcggcg gctcgcaggg
     7321 aagcatttgc ccggcgctct ccagcgccgc ttcaatcgcc accagcggcg ttccggcacg
     7381 agcggttatc accagctcgg tcgggtcgta attaacaatg ccgcgatgac aacgcacatc
     7441 cagcgtttgc ccggtgactg ggcgacctaa aaaggctttg ctattgctgc cctgaatcac
     7501 cagcggcgtt ttatcgctaa ttgcctgatt cacctgctcc agcagcgcct gactgtaatc
     7561 acactcgcgt agcatcaaaa gcgctccagt tcaggaaaag gtaaatgacc gtgatgcaca
     7621 tgcatggcac caaattcagc acagcggtgt agcgtgggaa tgtttttccc agggttcagc
     7681 aaaccatcgg ggtcaaacgc cgccttgacc gcatggaagg tcgtgatttc atcgctgttg
     7741 aactgggcgc acatttgatt gattttttct cgcccgatgc catgttcccc actgatgctg
     7801 ccgccaactt caacgcagag ttcgaggatc ttcccgccca gctcttccgc gcgggcaaat
     7861 tcaccaggtt cgttggcatc gaaaaggatt aacgggtgca tgttgccatc tccggcatga
     7921 aagacgttgg caacacgtaa atcatattgc tgcgataaac gggcaatgcc ttccagtacg
     7981 ccaggcaggg cgcgacgcgg gatggtgcca tccatgcagt agtaatccgg ggagatacgt
     8041 cctaccgccg ggaacgcatt tttgagaccg gcccagaaac gtacgcgctc tgcttcgtcc
     8101 tgtgccaaac ggacgtcagt cgcgcccgct ttcaacaaga tgtagttaac ccgctcgcag
     8161 tcttcctgta cgtcagactc cacgccgtcc agttcgcata acaaaatcgc ttcggcgtcg
     8221 acgggataac cggcatgaat aaaatcttcc gccgcgcgga tcgacaggtt atccatcatc
     8281 tccagcccgc cggggataat gccattggcg atgatgtcac caaccgcaag tccggctttt
     8341 tctaccgagt caaagctggc taacagaacc cgcgccacgg gcggcttcgg cagcagtttt
     8401 accgtcactt cggtggtcac gccgagcata ccttccgatc cggtgaacag cgccagcagg
     8461 tcaaaaccag gtgaatccag cgcgtccgat cccagcgtca gcgcctcgcc gtccagcgtt
     8521 tgcacttcaa ttttcagcag gttatgtacg gtcagaccat atttcaggca gtggacgcca
     8581 ccggcatttt ccgccacatt gccgccaatt gaacaggcga tttgtgagga aggatccggc
     8641 gcgtaataga gattatgcgg cgcaacggcc taggagatcg ccaggttaca tacgcccggc
     8701 tgcacgcgcg cgcggcgacc aacggggtta atgtcgaggc tctctttaaa gcgcgccatc
     8761 accaacaaca cacctttttc cagcggcagc gcgccaccag aaagcccggt gcctgcacca
     8821 cgggtcacca ccggtacacg caggcgatgg cagacagcca gaatcgctgt cacctgttcc
     8881 atttgcttag gcagaacaac cagtaatgga cgcgtgcgat acgcgctcaa cccgtcacac
     8941 tcgtaaggaa tgatctcctc atcggtatgc aggatctcaa gtccagggac atgctcacgc
     9001 agtgccatca gtaccgatgt gcggtcgaca tcgggtaaag cgccatcaag acgctcttcg
     9061 tacaagatgc tcatgagtag gcttcgcttt gttgtgttgt gtggcagctg atttttgcgc
     9121 gctgcttctg tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc
     9181 ggtcagacca ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg
     9241 atgtaacata atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt
     9301 cctacctgtg cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt
     9361 gcagagagta tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc
     9421 tcggaacgtc gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg
     9481 accgtgctgc gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca
     9541 cggcttaatc gggagcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga
     9601 acgctgtacg atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg
     9661 gcaacgctgg gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc
     9721 gccgccagtg agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct
     9781 ttccatctcg ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca
     9841 ttgaccgatc tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca
     9901 cagcaaaaaa agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg
     9961 ctgccgcacg tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc
    10021 cacgatatcg agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac
    10081 aaggtgggaa tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac
    10141 ttaacaggat ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc
    10201 gctaaacagg atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc
    10261 cgccccaatg cgttccagca tgttattcat gcctgtattg gtttcgagga tccatgacat
    10321 ctccagcgca tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag
    10381 cgcaatcacc gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac
    10441 tcgcgcagtt cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg
    10501 aaagagcgat ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cacaataaac
    10561 gcgcagggtg cagaatcaat ctcagcgatg tagatcatat cgtccggcac cagatattta
    10621 agttggtcgc ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc ccagttgtgc
    10681 tgccagccag agttgaaaat ctcacgcagg atctgcatct cttcggcaaa ccgctgacga
    10741 ttgatgcagc gaatggtcac ctttttgcgc acctgatcca tcagtttttt tagcgccgga
    10801 gagaaagtga gatcggttcg ctgcatccac cacgccagta aatcaatgcc tttgtgataa
    10861 cccagttgtt caatatgcgc ggcataccac ggtttgccgt gtggcatcat cgcacagggt
    10921 ggtgtgtcaa aaccttcaat cagtaatccg ctttcctgat tgatattcag gctgaaaggc
    10981 ccgctgatct tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc aaacaacgcg
    11041 gcaaaatcct gcggatcatc aatggcgtca atcatgccga aatgaccggt atcttcaccg
    11101 taacgctcac ggtgcaaggt atctatttgc gcggtaatac gcccaactat ctgccccgct
    11161 ttttttgcca cccacgcctg ccagatgata tggtccgtcc ccggattttt cgcagacaaa
    11221 tgctcgttgc gttcaatgaa taaagggggt atccagtttg gatcgtcggg ataaagtgat
    11281 gacggaaaag cgataaatgc cttaaggtca tttttattaa ttactttttc aattttaatc
    11341 atcagcagat cgcattccat atctctgtgg ataacaaata attaatggtg aaaaacaggt
    11401 tgcaacagca acgagcggca tcttaataat caactcaatg aatacaaacg acgaataaat
    11461 agtattaatt aatgattatg ataaggtcag aggctacaca gaaacgcctc aatgcctttt
    11521 gtcaatttaa ggcgaccgga caaggcttct tcttaatata ttcataaatt aaatatcatt
    11581 cataataaaa acaaaaaact taaactcatt atttaaatca atgttcattt tcacaccgat
    11641 gaaaatacta tctcatttat cttttatgcc tatataatcc accgcattat tgtcatttta
    11701 tttttgatat ccaatttttt cgtggtgtat atgtctaata aaatctttac gcattcccta
    11761 cctatgcgct atgccgattt tccaacgctg gttgatgctt tggactacgc cgctctgagt
    11821 agagccggaa tgaattttta tgacagacgt tgccaacttg aagatcaact ggaatatcag
    11881 acattaaaaa cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa cctgaaaaaa
    11941 ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc tttttttgcc
    12001 tgtcagtatg ctggcttagt cgccgtcccg ttggcgattc caatgggcgt cggtcagcgg
    12061 gattcctgga gcgccaaact ccagggttta ctggcaagtt gccagcccgc agccattatc
    12121 actggtgatg agtggttgcc actggtcaat gccgcgacgc ataacaaccc cgaattacat
    12181 gttttaagcc acgcttggtt taaggcatta ccggaagccg atgttgcgct ccagcgtcca
    12241 gttccgaacg atatcgccta cctccagtac acctccggca gcacccgttt tccccgtggc
    12301 gtcattatca cccatcgcga agtgatggct aatctacgtg ctataagcca cgatggcatt
    12361 aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga tatgggactg
    12421 gtcggctttc tcctgccccc cgtcgccacg caactttcag tagattattt gcgcactcag
    12481 gattttgcca tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg cggcaccgtt
    12541 tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga aaaagatctc
    12601 gctgaactgg atctttcctg ctggcgcgtc gctggtattg gtgctgagcc gatctccgca
    12661 gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta actttgacaa taaaactttc
    12721 atgccgtgct acggactggc agaaaatgcg ctggctgtca gcttctctga tgaagcctcc
    12781 ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa agccgtcgcg
    12841 ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc gttgccggaa
    12901 catggtattg aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt ggtaggccat
    12961 atttgcatct ccggtcccag tctgatgagc ggttactttg gcgaccaggt ttcgcaagac
    13021 gagattgccg cgacgggctg gttagacacc ggcgacctcg gttatctgct ggacggttat
    13081 ctgtatgtca ccggacgcat taaagatctg attattattc gtggccgtaa tatctggccg
    13141 caggatattg aatatatagc ggaacaggaa ccggaaattc attctggcga tgcgattgct
    13201 tttgttaccg cccaggaaaa aatcattttg cagatccagt gtcggatcag cgacgaagaa
    13261 cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc aaagcgaatt tggcgtgacc
    13321 gcggctatcg atctgttgcc gccccacagt attccccgaa cgtcctccgg caagcctgcc
    13381 cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa tgtgcaggaa
    13441 tccctggcat aaatcaaact gtcgcggtga cgggcgctac cgggtttatc ggtaaatata
    13501 ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt actgcccgcg
    13561 ctcacgtcaa tgataatctc acctgggtgc gcggttcgct ggaagatact cattcactta
    13621 gcgagctggt tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg cgcgggcaca
    13681 aagaagagat tttcacccgc tgtaacgttg acggcagcct gcgcctgatg caagcagcaa
    13741 aagagagcgg cttttgccaa cgttttctgt ttatctcttc gctggcggcg cgccatcccg
    13801 agctctcctg gtatgcaaaa tccaaatacg tcgccgagca acgactggca gcaatggcgg
    13861 acgaaattac gctgggcgtt tttcgcccga cagccgtgta tggtcccggc gataaagagt
    13921 taaaaccgct gtttgactgg atgctacgcg gcctgctgcc acgacttggt gcgccagata
    13981 cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag tggttaagcg
    14041 ccgaaactgt acagacgcaa acctatgaat tatgcgatgg cgtcgccggt ggctatgact
    14101 ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg ttccgtgcga atggttgaca
    14161 ttcctctgcc ggtactcaca ggccttgcgg atatcagtac cgcgttgagt cgcctggcag
    14221 gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc gactggtcgg
    14281 caagtaataa ccgtatttct gaagatatca attggtttcc cgggattagc ctggaacacg
    14341 cattacgcaa cgggctattt tgattgtctt gttttaaagg tgtgttaacg tttctatggt
    14401 aaatcgtgaa atagtaatgg attacatcct ttcctgttta caggatttgg tcgaaaacgg
    14461 tgtggaaatt aagcccgaca gcgatctcgt caacgatctt ggcctggagt cgatcaaagt
    14521 gatggatctc ttaatgatgc tggaagacag attcgatatt tccattccta ttaatattct
    14581 gctggatgtc aaaacccctg cgcaattaat ggaaactcta cttccatggc tggagaataa
    14641 ataatggggc tatacgataa atatgcgcgc cttgctggcg agcgtctgca atttagtgac
    14701 aacgggttaa caccgtttgg cacctgtatt gatgaagtgt attccgccac cgaaggccgg
    14761 atcggcaata aaaaagtgat tctggccggg accaataatt atctcggttt aacctttaat
    14821 catgatgcca tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg caccaccggt
    14881 tctcgtatgg caaacggcag ttatgcgccg cacctggcgt tagagaaaga gattgccgag
    14941 tttttcaatc gccctaccgc tatcgttttt caaccggtta taccgctaat ttaggcgtca
    15001 tcagcgcact tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta
    15061 tttatgacgt ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta
    15121 aagatctgga acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg
    15181 ttgagggcat ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca
    15241 aacgtcgtct cggtggttat ttgattgtcg atgaagcgca ttcgtttggc gtgttaggcg
    15301 caacggggcg cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg
    15361 gtactttcag taaaagtctg gcctcgattg gcggttttgc tgtcgtttcg gaagcgatgg
    15421 aagtgctgcg ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca
    15481 ttgcgaccgt gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc
    15541 tgatggataa cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt
    15601 cacatattag cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct
    15661 ggcgcgagct tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg
    15721 caggcatcac gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg
    15781 ccatcattca ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc
    15841 cacggcgggt ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa
    15901 acgctaccgg taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat
    15961 taaccatgat ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac
    16021 ggctacccat cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca
    16081 tcgccagggt caggatgggt tgccccagtt tttcccacag tgcaaggtgg tattcgctgc
    16141 tgggttgccc ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag
    16201 tattccccgg cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa
    16261 gattatctaa tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat
    16321 gcaggaccca cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa
    16381 gcgagagatc gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca
    16441 gcgatttcac cgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt
    16501 catcgtcctc ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg
    16561 gcgacgccac ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca
    16621 gcgccaccag tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac
    16681 tgtttttcga taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa
    16741 gttcgaccag tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat
    16801 aaccgcccgg gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca
    16861 gcaatagccc tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga
    16921 aaacattcat cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag
    16981 gcccattagc atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt
    17041 agccaccagt gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag
    17101 tggcaataac gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag
    17161 cagcgccatc aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc
    17221 ggcaggaaag gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg
    17281 gctttgatcc agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc
    17341 acgcccctga tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc
    17401 gggtgatggg tcaaccacat caaccgaacc ggcgcggaaa atacgggttc gattggcagt
    17461 agaagtataa atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag
    17521 gatcattcgt ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag
    17581 ctccgactgc gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata
    17641 catcgacaga agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta
    17701 caagcgtccc ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat
    17761 ctgccctagc gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc
    17821 aatcagtact ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc
    17881 cgcttcggtc agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac
    17941 gataatcagc accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc
    18001 actaacgccc tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat
    18061 gacatgatct acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc
    18121 tttattcagc accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg
    18181 tccattgccg tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt
    18241 gccaacaaca aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg
    18301 ctgaaaacga tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt
    18361 tttcttcgcc gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg
    18421 tgtgctgttc cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc
    18481 ttcctggata taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc
    18541 cggggctaag agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc
    18601 tatagcggaa aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc
    18661 aacggtgcat aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta
    18721 tctcccatca ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt
    18781 tcaggatcaa gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc
    18841 acatcatgaa attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc
    18901 atttgctttt gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat
    18961 gaatagttca ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa
    19021 tacgacacaa tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg
    19081 gtcgttttgc cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa
    19141 cgcaggtgct tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc
    19201 tcatcgcctt tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg
    19261 agccagaatt gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt
    19321 gcaatagtcg gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca
    19381 aatagacgct cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg
    19441 ctgcgttata aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga
    19501 gcgccacgtc cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca
    19561 gatgacagaa catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga
    19621 gaaaagcgcg ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg
    19681 aaatcacaat tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc
    19741 caaacaccag cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac
    19801 cgattccgcc cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg
    19861 gatctaaacg catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg
    19921 cggcgtcgaa gaatgtcatg gcgattttaa ataacccggc ggcagcgggt cctaacacga
    19981 tccccaccaa cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg
    20041 ttgaccagac aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt
    20101 tgaaggcgtt atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg
    20161 tgccgccaac cagattcgac acgtaccaag caataacaaa acccacaaaa ccaaagtcaa
    20221 aataccaggc tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag
    20281 caatcaaatc aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg
    20341 aaggaatgag cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg
    20401 aatgggaaag gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga
    20461 tatccagcga gaaggaaaat gagacgacat tgcgaaattg ctgcggatta ttgttggtca
    20521 aggctggcgt tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga
    20581 tcgacttggc gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt
    20641 taccggcaca cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta
    20701 accaggcgct attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt
    20761 taaaacccgc caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt
    20821 aagttaaact tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt
    20881 ccagcacttc gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc
    20941 caatcgtcga catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc
    21001 gggagatggc agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg
    21061 ccattttttg gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg
    21121 cacgttcgac gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga
    21181 agcgatcgct gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg
    21241 ccattcttcg cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg
    21301 ttttcatgct ttgggttttc gaggatttgg ccgccaatcg ccgttccagc cagacgccaa
    21361 ccaacggcaa tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc
    21421 gctcggtttg ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac
    21481 cggtgccatc acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg
    21541 ttgtactatt aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat
    21601 tttggtgatc aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg
    21661 gctggttgcc gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta
    21721 acggcgattt tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt
    21781 tgttcatcaa tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac
    21841 agcttcaact cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc
    21901 aattgcgggc catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg
    21961 agcagatagc cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag
    22021 gaaagcagat agataaccag cgcagtaata ttgccaggcg gagttaaggg cttttcgtgt
    22081 acatgtgccg ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt
    22141 gaaatccatt cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt
    22201 cttgctgcca gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat
    22261 cctacaatgg caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc
    22321 acgaaaaacc cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc
    22381 gttcaacctt attgtgacaa tgactaattg tgacattgac ctgaattacg ctttcataaa
    22441 aacatattaa ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca
    22501 gtcaataaca cctccattaa ttgccgttat tggtagcgat gcttcaggca agtcaacggt
    22561 gtgtgaacat cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg
    22621 aaaacaggcc ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt
    22681 acataaaaca attgaacgaa atcaggtgaa aacagcaaaa aaattgcctg gaccagttcc
    22741 ggcgctggta attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc
    22801 ctgtcgtcgt cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg
    22861 cgcttacgat ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct
    22921 ggcaagccag gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat
    22981 caaactcaat gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc
    23041 gctggcgagg aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga
    23101 tatcgatgcc aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga
    23161 ttttatgacc gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg
    23221 ggcattttca ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa
    23281 aaattgccgc cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct
    23341 gtaacccacc gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg
    23401 tggagacagg catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg
    23461 ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg
    23521 ctacacgacg ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga
    23581 tccacaccgg agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt
    23641 tctggtcttc tttactgacg cctggcagtt tcgccagctt cgcggaggta tcggagatgc
    23701 acagcataat gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca
    23761 tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac
    23821 agtgaaactc tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg
    23881 caatcaactt ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg
    23941 catcgcgggt acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag
    24001 gggcaatccc caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcgcgc
    24061 cgtgcgaaaa cgccacgccc gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg
    24121 gtttacgttt gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct
    24181 ttttcgtccc actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc
    24241 cgacgatagg ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg
    24301 catccatcac cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac
    24361 caatcaaggt gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga
    24421 taatcgccgc cagcagcatg gaaaagacca tcgccaaacc gtgagttgac cccatattca
    24481 gcaacaaatc gattggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata
    24541 acacgccaaa aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac
    24601 gagtataaat aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt
    24661 agaacaacac aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa
    24721 caaataaatt tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc
    24781 agtgacttta acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta
    24841 tttcttgtgt atcagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag
    24901 gtattccact ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat
    24961 tgaatatttt gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca
    25021 acatgtatta ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca
    25081 gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata
    25141 cctgaatgta tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct
    25201 gctcggcaaa tttaccgctg gttttgtcca gcacctcttc atgatggctg accaggtcga
    25261 tattgctacc acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat
    25321 cattaacagt gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga
    25381 tcggcagaat cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt
    25441 ccgggcgcag caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg
    25501 gcaccgcagc aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg
    25561 cccaggtttt ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag
    25621 catcccagcc cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca
    25681 gcgcctgctc cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat
    25741 ggacaaacgg acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt
    25801 ccgccggatt gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc
    25861 ccgcttcggt atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct
    25921 catccatgca gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga
    25981 gacgcaaacg tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt
    26041 tcagcacctt gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta
    26101 gctcctgctc ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct
    26161 gaccgttggc ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt
    26221 caaactggcc tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt
    26281 ccggctgcgg taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg
    26341 tgtcatcaaa agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc
    26401 gcgtccactg ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga
    26461 ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc
    26521 ctttatccca gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg
    26581 cctgcaatgg caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct
    26641 cccacgccat acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag
    26701 caaattcaac gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata
    26761 cggcgtcatc ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta
    26821 ccccacctgg ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt
    26881 tgctcatcac tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg
    26941 attacctgct cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag
    27001 cggctggcgc aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc
    27061 ttcaacacta ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa
    27121 agtctggacg tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc
    27181 tggtccgacg catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct
    27241 gggaacgccg aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt
    27301 tactggtgca gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag
    27361 cggctttgtc gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa
    27421 tccgccgatc cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg
    27481 ctacttcctg ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt
    27541 acagggtgcc gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt
    27601 gaaaattgag tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct
    27661 ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat gagtacacca ttgcggatat
    27721 ggcgatttgg ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt
    27781 tctggatgcg ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc
    27841 ggcagtgaaa cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca
    27901 tgagcgccat gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg
    27961 gttggtgttc gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca
    28021 atcgtgcaca atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca
    28081 tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc
    28141 atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag
    28201 atcggcaggg ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac
    28261 ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc
    28321 aatacacatt gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta
    28381 gcgggtgttc cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc
    28441 tttttaggcg ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc
    28501 cggtatcgac acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa
    28561 tctccaccac ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta
    28621 aatcgcaccc ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc
    28681 tctcctcaac gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat
    28741 cgtgctgctc cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat
    28801 gcattttcgc ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc
    28861 ggattggtga ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct
    28921 tcttcaatcg acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa
    28981 acaccgtcca gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt
    29041 ttttcactca ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc
    29101 agcatccacg gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa
    29161 acatacaccg gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt
    29221 tcttcaaacg ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca
    29281 gtcatgaatc gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac
    29341 ctgctcaagc gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc
    29401 cagcgattcc gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg
    29461 cagggccgac aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa
    29521 cgccggaact tcttcatccc gcaggatcat catcgttccc ggagtgttct ttttcgacac
    29581 aatggcatcc gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc
    29641 cgccgtgccg ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc
    29701 ttcgacaatc cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac
    29761 taaaatacgc attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg
    29821 tgtaccgcac aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt
    29881 ttattcggat cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca
    29941 ttgaagttac gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg
    30001 ataccgtctt taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca
    30061 acacctttga actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct
    30121 ttgccgatat tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag
    30181 cagtgaacgg tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc
    30241 gtgttgccag tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat
    30301 tcgcgacctg ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg
    30361 ccgtagaaag tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa
    30421 gccggaatgg tggtaccttc ccacggcgcc tgtggcgctt cgtctttata ccaggagtgc
    30481 ttcgcgcttt cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga
    30541 tacgaggaca gatccgcatt ctcaatgtag cctcccggga acaggaagct gccgtttttg
    30601 ctgtcggtcg ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc
    30661 cattccgggt agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag
    30721 tcgctcagtt tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc
    30781 aacccgtcga ggttgattgg gttcgcgaca ccacccacag ccaggttctg aatgtgcggc
    30841 gttttaccgc ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc
    30901 aggtagtgcg ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc
    30961 cagtagccat tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc
    31021 tgaactttag tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt
    31081 tcggaggctt tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc
    31141 gaaagctgat agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta
    31201 cggatgtatt gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg
    31261 gaagacagcg cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca
    31321 tcgcgcggat cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc
    31381 catgctttcg aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata
    31441 cgggttaccg gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat
    31501 gatcgttttg ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc
    31561 aaatctcaat agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg
    31621 cgtaaccgcc gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac
    31681 ctaacagtgc gctcagtgct gacaggaaca gcatacggga atcattacgc agcttcgcca
    31741 cacgcagaac gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt
    31801 agaagtcacc ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca
    31861 gcccgatgaa aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct
    31921 tttcatccgg accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa
    31981 tcgagaagcc catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct
    32041 gccagagcgg atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt
    32101 gcatggtcgg cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt
    32161 gtagcgacac cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca
    32221 cgccgatgta gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt
    32281 gacccggaat gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca
    32341 agccacccag tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt
    32401 attgcccacg gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc
    32461 cggtgccaat cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt
    32521 cagagacaga gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga
    32581 ttaacggtcc aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat
    32641 catgactcat gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc
    32701 acggttaagc ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga
    32761 atattttcgg aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca
    32821 taaggcacac ccgtcagtac cagaacctga gtaccgccgc cctctttctc gccgtacaga
    32881 tgcggataat atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc
    32941 ggatagtggt attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc
    33001 tcttcacgcg taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga
    33061 ccgcctttat cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca
    33121 ccaaacgggt tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa
    33181 cggcaaccgg tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc
    33241 agtgcagaga ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc
    33301 ttaatgtacg cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac
    33361 acctgaatga tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc
    33421 ccttgcgggt tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag
    33481 cctacgcaca aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt
    33541 tcagcagccg catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct
    33601 gctttaataa aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct
    33661 tttgctgacg acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca
    33721 caccgccgag caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat
    33781 ccggtttctg tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat
    33841 ggaagccgat acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca
    33901 caccgccaac atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac
    33961 agccgaggtg gtacaagcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa
    34021 aacgaccagc atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg
    34081 cgaaggtcgg acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg
    34141 caacggtcgc gaggaagttg tgcgggttcg gcgggcagcc cggaatattg ataacggttt
    34201 tgcctggcag aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa
    34261 caccgcccca cgcggagcag gaaccaatag cgataatggc tgctgagcct tccgccgctt
    34321 tgcggatgtg atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta
    34381 atgggatgga accatccacc actaacacat actgcccttt gtacttctcg agagcgttat
    34441 gtttgttctc ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag
    34501 agatagtctc cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg
    34561 tacaaccggt gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa
    34621 ccgattcggc catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg
    34681 cacaaagctt catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc
    34741 cagtcattta tagttattcc gttgcgagaa cctggcatat attttgcctc aatcgcaaaa
    34801 tcaataatgc gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat
    34861 actgcggcgg ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat
    34921 aatacgtatg tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga
    34981 acaggaatta ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg
    35041 cgtcagaata cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatcct
    35101 gtgtgagtag cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac
    35161 cgcactgtcg gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt
    35221 catcgttcac ctcattcatt atatatcgcc atatataacg atattttagg caaagaacga
    35281 cttttgcgca cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct
    35341 atcaggagag atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag
    35401 atttgtcata acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat
    35461 aagcctcata aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt
    35521 tgattcgggt gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat
    35581 cggcagtggg tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga
    35641 cagccgccgc gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg
    35701 tcgcttgcgg atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg
    35761 ttggatcaac ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa
    35821 cggaacttaa tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca
    35881 catcttcgat atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt
    35941 tgccgctcat ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg
    36001 aaggatacgt gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca
    36061 gcgcataatt tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat
    36121 acttcgccgc aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt
    36181 agtaatccaa caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc
    36241 tcaactggtg tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact
    36301 ctgtgagcca gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga
    36361 aattgaacgg tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca
    36421 gatgcagtac cgctattgcg gaaaaagtgg tttacgcctg cccgcattat cgctcggttt
    36481 atggcacaat ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc
    36541 gtttgattta ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag
    36601 cgcagaagag aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact
    36661 gattatctct accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc
    36721 acgtaaatac ctgctcgcca gcctcgacca aagcctgaag cgtatggggc ttgagtatgt
    36781 cgatatcttt tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc
    36841 gctggctcat gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc
    36901 agagcggacg caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca
    36961 tcaaccttcg tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct
    37021 gcaaaataac ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg
    37081 aaaatatctc aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaagttcg
    37141 tggtctgacg ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga
    37201 aatggcacag cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga
    37261 tgatcgcgtg acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt
    37321 gcaggcgctg aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat
    37381 cgccgatggc gagctgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg
    37441 gcgcgcgaac cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc
    37501 ccatcacaaa tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt
    37561 tatccgggac atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca
    37621 caatcgacgc tgctactttg tttttcagcg acgttgcgtc cattttcccc agccacttca
    37681 gcttctcttt gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca
    37741 tcaccatcac cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga
    37801 tcaaatctga ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga
    37861 acttcagcgc cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc
    37921 agcgagaagc atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta
    37981 aaacgggcaa cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac
    38041 gttaaacctt tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc
    38101 atcgagagcg tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt
    38161 taaaagaccc gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc
    38221 caggcatcca ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta
    38281 gcggtcgcct gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg
    38341 ctgccgccat cgcttacgcg cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg
    38401 aagaagaaga cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc
    38461 tgctgccagg cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca
    38521 aggcgttagg cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg
    38581 atattgcaga cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc
    38641 tgttctggct aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt gtcatcacca
    38701 cttcgtcaat ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg
    38761 cggcgattct gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg
    38821 tgaatattgt cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc
    38881 aggataagat cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg
    38941 aactggcccc tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag
    39001 tgcacggcgt gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa
    39061 ccgggcatca ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca
    39121 aacgcatgaa gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc
    39181 aggcattgag cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac
    39241 gaagattcaa catgttgcaa tagattttgt aggcctgatg agcgtagcgc atcaggcaat
    39301 tctactttac ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc
    39361 ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg
    39421 gaagaagatg gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt
    39481 ttcatcggtg accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata
    39541 aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac
    39601 atctaccgtc gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc
    39661 gataaacggc gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag
    39721 atgcattgcc atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc
    39781 agcggcgctg gcttccagat ctaggtcacg gctttgcagc aacaatacct gcgctgcaac
    39841 atcacccagc atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac
    39901 cgcaggaatc gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg
    39961 cgcaacgact gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat
    40021 gccccagacc gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata
    40081 gccgttaccg cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct
    40141 ggtacgttct ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg
    40201 cgcttcattg agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt
    40261 ggcctggttt aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt
    40321 ctgattgaag aactctacgc tcttactgaa gaagattgcc caggtgacta cagaggccaa
    40381 aataagccca atcatcacgc acttaacgac aatatcggcg tactgataca taccccagac
    40441 ggaaaggtcc gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa
    40501 aatctgcgca taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg
    40561 catactgccg tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca
    40621 cgccatttgc gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc
    40681 atgctagttt agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc
    40741 ttgatactca actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata
    40801 gcgtgattca gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga
    40861 cgcgcaatcg cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt
    40921 ccttacaaca agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg
    40981 gggcggcagc ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga
    41041 tgaccaacac cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg
    41101 gcgtaacgac atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc
    41161 caaacactaa aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg
    41221 ttccggcgat tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca
    41281 acacctgggc agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc
    41341 aggccgccac caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca
    41401 atgcccgttg ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg
    41461 ccgataccgc ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac
    41521 atcatgaaag cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgcgcgag
    41581 ttaaccaccc tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag
    41641 gcagcagcgg gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga
    41701 actatctgga taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt
    41761 tgatcctggc aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt
    41821 ttagcgggac cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg
    41881 atctggacgc cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga
    41941 cattttattg tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc
    42001 aggagtacaa taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt
    42061 attcaagata tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat
    42121 attgttagcg ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg
    42181 cccgcctcat ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa
    42241 ggggttatgg attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc
    42301 tggctaagtt atattcaggg gagctggtta gggaatacca aaacggtgaa aggctggctg
    42361 gcacagcttc ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg
    42421 ctggcgctgc tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg
    42481 ggaatttccg gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg
    42541 tgggtcagcg tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc
    42601 catgaagatc aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc
    42661 ttgttaggca cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc
    42721 ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc tgcatcgccc gcattcttgc
    42781 cgcatcttcc cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc
    42841 gctttcatag ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat
    42901 catccgcgcc ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac
    42961 agacttaaaa ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc
    43021 gacgctcagg ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact
    43081 gaagtgagtc tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac
    43141 gtagtacaga atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc
    43201 catcacgtcg agtaaccgct ccgccgcgca taaaatctct tctgataacg tggcggagtt
    43261 aatcccgctg gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag
    43321 ttcctgtaac tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt
    43381 ggcataggtt tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc
    43441 ataacgaaac acgcgttcat tgatataacc gattttatgc ccggaaaaga gaattatgat
    43501 gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac
    43561 atcgggcaac agttctgaca aactattttc tttatttttc agtttattaa ctttatccgc
    43621 cagcaagcga caaatctctt cacgtttcat atcgcgtaat ttcttaggaa taatgcggca
    43681 atttgattgt gcgcaatttt gtagcatttc tccagcactc tggagaaata ggcaagacat
    43741 tggcagaaat gagcattgag agccagggcg ctggcgatca caatgaaaaa catcaggcag
    43801 atcgttctct gccctcatat tggcccagca aagggagcaa gtaatgaaca actttaatct
    43861 gcacacccca acccgcattc tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat
    43921 tcctcacgat gctcgcgtat tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt
    43981 tctcgatcaa gttctggatg ccctgaaagg catggacgtg ctggaatttg gcggtattga
    44041 gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt
    44101 gactttcctg ctggcggttg gcggcggttc tgtactggac ggcaccaaat ttatcgccgc
    44161 agcggctaac tatccggaaa atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga
    44221 gattaaaagc gccatcccga tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc
    44281 caacgcaggc gcggtgatct cccgtaaaac cacaggcgac aagcaggcgt tccattctgc
    44341 acatgttcag ccggtatttg ccgtgctcga tccggtttat acctacaccc tgccgccgcg
    44401 tcaggtggct aacggcgtag tggacgcctt tgtacacacc gtggaacagt atgttaccaa
    44461 accggttgat gccaaaattc aggaccgttt cgcagaaggc attttgctga cgctaatcga
    44521 agatggtccg aaagccctga aagagccaga aaactacgat gtgcgcgcca acgtcatgtg
    44581 ggcggcgact caggcgctga acggtttgat tggcactggc gtaccgcagg actgggcaac
    44641 gcatatgctg ggccacgaac tgactgcgat gcacggtctg gatcacgcgc aaacactggc
    44701 tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc aagcgcgcta agctgctgca
    44761 atatgctgaa cgcgtctgga acatcactga aggttccgac gatgagcgta ttgacgccgc
    44821 gattgccgca acccgcaatt tctttgagca attaggcgtg ccgacccacc tctccgacta
    44881 cggtctggac ggcagctcca tcccggcttt gctgaaaaaa ctggaagagc acggcatgac
    44941 ccaactgggc gaaaatcatg acattacgtt ggatgtcagc cgccgtatat acgaagccgc
    45001 ccgctaagct ttttacgcct caaactttcg ttttcgggca tttcgtccag acttaagttc
    45061 acaacacctc accggagcct gctccggtga gttcatataa aggaggaacg tatggctaat
    45121 ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc agctgggact gggcgtctgg
    45181 caagcaagta atgaggaagt aatcaccgcc attcaaaaag cgttagaagt gggttatcgc
    45241 tcgattgata ccgccgcggc ctacaagaac gaagaaggtg tcggcaaagc cctgaaaaat
    45301 gcctcagtca acagagaaga actgttcatc accactaagc tgtggaacga cgaccacaag
    45361 cgcccccgcg aagccctgct cgacagcctg aaaaaactcc agcttgatta tatcgacctc
    45421 tacttaatgc actggcccgt tcccgctatc gaccattatg tcgaagcatg gaaaggcatg
    45481 atcgaattgc aaaaagaggg attaatcaaa agcatcggcg tgtgcaactt ccagatccat
    45541 cacctgcaac gcctgattga tgaaactggc gtgacgcctg tgataaacca gatcgaactt
    45601 catccgctga tgcaacaacg ccagctacac gcttggaacg cgacacacaa aatccagacc
    45661 gaatcctgga gcccattagc gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc
    45721 gatctggcag ataaatacgg caaaaccccg gcgcagattg ttatccgctg gcatctggat
    45781 agcggcctgg tggtgatccc gaaatcggtc acaccttcac gtattgccga aaactttgat
    45841 gtctgggatt tccgtctcga caaagacgaa ctcggcaaaa ttgcaaaact cgatcagggc
    45901 aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac gtgcaaattc tcccggtggc
    45961 ggtaatgttc cgctaccgga cttttcagaa atcatttatt cccctcgcgt cccgcccgtt
    46021 gttactcttc cttgttcagg aatgccaaat ataaggacat catcatgcag agccggaagc
    46081 tcttaaaaga acaactcatc tatatccggg ataaacgcaa cggagaggtg aaaaacagat
    46141 gaaaataata cttctgtttt tagcagccct ggcaagtttt accgtacacg cgcagcctcc
    46201 ttcacagacc gtagaacaaa cagtccggca gatttatcag aactataaat cagatgccag
    46261 tacgccttat ttcggtgaaa ccggagagcg ggcgataact tcagcgcgta ttcaacaggc
    46321 gcttaccctg aacgacaatc ttacactacc gggcaatatt ggctggctgg attatgatcc
    46381 ggtttgtgat tgtcaggatt ttggcgatct ggtgttagaa agcgtagcga taacccaaac
    46441 tgacgtcgat catgccgatg ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa
    46501 gaccacacag acactgaaaa tggtggcgga aaatggtcgt tgggtcattg atgatattgt
    46561 cagcaatcat ggcagcgtct tacaagcagt taatagcgag aatgaaaaaa cgctggccgc
    46621 tttagcttcg ttgcagaaag aacagccaga atcctttgtt gctgaactct ttgaacatat
    46681 tgctgattat agctggccgt ggacgtgggt ggtttccgac tcttaccgcc aggcggttaa
    46741 tgccttctat aaaaccacct tcaagacggc caataatccc gatgaagata tgcaaataga
    46801 acggcaattt atttacgaca atccgatctg ttttggcgaa gagtcgctat tttcacgcgt
    46861 tgatgaaatt cgagtcctgg agaaaaccgc cgattccgcc cgcattcatg ttcgttttac
    46921 gctgaccaat ggcaacaatg aagagcaaga gctggtttta cagcggcgcg aaggtaagtg
    46981 ggaaatcgct gattttatcc gcccgaacag cggcagccta cttaagcaga ttgaggcaaa
    47041 aaccgccgcc agattaaagc aatgagctga attaaataac aattagccgg aacaataaat
    47101 aaaagggaac actatatgaa aacgattttc accgtgggag ctgttgttct ggcaacctgc
    47161 ttgctcagtg gctgcgtcaa tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca
    47221 ttaaatgcca aaatcgcccg gcttgagcag gatatgaaag cactacgccc acaaatctat
    47281 gctgccaaat ccgaagctaa cagagccaat acgcgtcttg atgcacagga ctattttgat
    47341 tgcctgcgct gcttgcgtat gtacgcagaa tgataaaaaa atccccggca gcatgtcagt
    47401 tgccggggat tttttttaac gtccaaccgc cgctttaggg cgtttcttcg caccagcatt
    47461 caccggacga gattgcgtag acgacgcttt ttttgccgta gcaggcgtct gacgctgagt
    47521 cgccatcggc gtatgtttag tcaacgccgg acgggtattg cggttctggc gacgagcttc
    47581 acgcatctct tcaatggttg gcgcaggcac taagcaatcg cgacggctgc caatcagatg
    47641 ctttttgccc atcgcttcca gcgcctggcg gattaacggc cagtttgccg gatcgtggta
    47701 acgcaacaac gctttatgca aacgacgctg tttgtcgccc ttcggtacga agacgccttc
    47761 gctcttataa ccaatcttcg ccagcggttt tttcccggtg taatacatgg tggttgagtt
    47821 cgccagcggc gatgggtaga agttctgtac ctggtcgaga cggaaacgat gctttttcag
    47881 ccacagcgcc aaattcacca tatcttcatc acgcgtaccg gggtgcgcag agatgaaata
    47941 cgggatcaga tactgctctt tacctgcctg tttcgagtag gtatcgaaca gctctttaaa
    48001 gcggtcatag ctgcccatgc ccggcttcat catcttcgac aacggccctt cttcggtatg
    48061 ttccggagca atcttcagat aaccgccgac gtgatgggtc gccaactctt tgatatagcg
    48121 cggatcttcc acggcgatgt cataacgtac gcctgaggcg ataaggattt ttttaatgcc
    48181 tttcagatca cgcgcacggc gatagaggtt gatcgtcggt tcgtggttag tgtccatgtg
    48241 cggacaaata tccggataaa cgcacgacaa acggcgacaa gtttgttcag cgcgtggcga
    48301 tttgcagcgc aacatataca tgttggcagt tggcccaccg agatcggaaa tcacgcccgt
    48361 aaaacctgga acggtgtcgc ggatcgcttc gatctcatta atgatcgaat cttccgaacg
    48421 gctctgaata atgcgccctt cgtgctcggt aatagaacag aaagaacagc cgccaaagca
    48481 gccgcgcata atgttgaccg aaaaacggat catttcgtaa gccggaatac gggcattgcc
    48541 ataggccgga tgtggcacgc gcttgtacgg cagcgcaaaa acgctgtcca tctcttcggt
    48601 agaaagcggg atagcaggcg ggttgatcca cacatagcgg tcaccgtgtt tttgcatcaa
    48661 tgcgcgggcg cagcctgggt tggtttcgtg gtgcagaatg cgcgaagcat gggcgtacag
    48721 cactttatcg cccttcactt tctcgaaaga aggcagcaat acgtaggttt tttcccacgg
    48781 tttctggcgc ggtggctgca cgattacagc tttcgcttcc tgctttttcg gcgcaacagg
    48841 tttgttatcc gcacacggca aatcttcgcc atacggatgc gggattgggt cgattttccc
    48901 tagggtatca agacgggtgg aatccacacc gctccagcca ggcagcgcct cttttacgat
    48961 aattgcggta ttacgcacat cgcggatttc actaatcggc tcgcccatcg ccagacgatg
    49021 cgctacttcc accagcggac gttcaccgtt accaaacatc agcatgtcgg ctttcgaatc
    49081 taccagaacg gaacggcgca cggtatcgga ccagtaatca taatgcgcgg tacggcgcag
    49141 gctagcctca ataccgccga ggatcaccgg cacatctttc cacgcctctt tacaacgctg
    49201 ggtataaacc agcgtggcgc gatccggtcg cttacccgcg acattatctg gcgtgtaggc
    49261 atcatcatga cgtaaacggc gatcggcggt atagcggttg atcatcgaat ccatgttgcc
    49321 agcagtaaca ccaaaaaaca gattcggttt acccagacgc ataaagtcgt ctttgctgct
    49381 ccagtccggc tgggcgatga tcccgacgcg aaagccctgc gcttccagca tacgaccgca
    49441 aatcgccatc ccgaagcttg ggtgatcgac atacgcgtcg ccagtaacca aaatgatgtc
    49501 gcagctatcc cagccaagtt gatccatctc ttcacgagac atcggcaaaa acggtgccgg
    49561 tccaaaacag gcggcccagt actgcggcca ggagaacagg tcgcgatccg gttggatcag
    49621 ggagatagag ctcattttgc ttccagaaat gataaaaaaa taatcaaagg ccggggatta
    49681 taagccggaa cgaaagagaa atcgaaaggt attccatact cgccctcctc gggcgagtat
    49741 gaagattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc cgccatttcc
    49801 agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc gaaataaaca
    49861 agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc ttccgggaac
    49921 ggcatcgcgc cattcacgtt acggatctgg aacattacgc cttcaatatg gaacgcttgc
    49981 ggctcgtccg cgcgtaccgt ccagcgttcc cacgttcctt gctgcgcggt gacatcaata
    50041 cggttgacgt cccacagctg tccattaata cccgggtcat cacccagact gatatcgcga
    50101 ctgcgaattg gcgaaccagc catgatttca gttggcagca agcgcatcgg aagactgtct
    50161 gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccaaggtaga aaccagaata
    50221 ctggatggct caaagaagcc gcgaatacga tcaacaatgc tcgccgcttc accacaggtg
    50281 atcgacactt catcgccgtt gctcatatcc accagaatct cgcggcgctc gcccggtgcc
    50341 agcgaaagtt gcttcaccga tacaggagca ggcaggaatc cctgatcgcc agaaatcaca
    50401 tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga cgcgttcagc
    50461 agtcgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac accgttaacc
    50521 agcagcgtat caccaacaaa gccgccgctt cccggttcgt tgtattctgg cgtaccaaag
    50581 ttatccagcc gtttatcctg gataatgacc ggaaaatcat ccacaccata atggttgggg
    50641 ataggcagcg acttgctgac ttcatcttcc accagccaca ttccggcaag gccgttatag
    50701 acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc tgcgttctgg
    50761 cgaataggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc
    50821 atcagcgggc ctggtacctg tagcccggcc accgtcattg agacattttc tgtcaggcgg
    50881 ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa
    50941 cgaccattga ttccccagac cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt
    51001 tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gtagcggcgg aacgggtagc
    51061 ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca aagtgcaatc
    51121 cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg taaaactggc
    51181 taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg acttaaactt
    51241 ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt tgttccatta
    51301 tcgaacgaca atgggcagcc agctcacgaa cctgatcttt gccatactga ctgacgtcaa
    51361 ttggcggcag catttcgaca atcaccagac cgttgtgcag tcgattaaga ttaatcttat
    51421 tcgaagttgt agagacgcac acgggaataa tcgggacgcc cgccgcaatt gccgcgtgaa
    51481 atgctccagt cttgaacggt agcaggccgc gaccacggct gcgggttcct tccgggaaca
    51541 tccagatgga aatacggcgt tttttgaaat gattcacgac ttccgcaatg gtgccgtgag
    51601 ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac aactgcccga
    51661 agaaggggat ccacagcaag ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt
    51721 tcgatgctgt caccatgtca tagttgttct ggtggttagc gatatagata gcattgccgt
    51781 agctttcagc atctgcaggt ttgcggcact caactttcag gccgaacagt ggcgcaagac
    51841 ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg aaaaggcagt
    51901 aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata agacgaaaga
    51961 tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca cctgaaatca
    52021 ggttagggca ttgttaaaag ccgctctctg gaaaagcgta ttgttaatcg caacgcatga
    52081 ataaacaacg gtttcaaggg aatttttatc gtttccgggg gttgaatacg ctgccggatg
    52141 acgacttaac gtttcatccg gcgttccttg ctagcgggag gaaacagcgc cctccccggc
    52201 atattactct tcgctatcac cgctgctggc acggcgagga gagtcgatct caacacgatc
    52261 gatacgctgc aaaccgcgca tcaacgtacc gcggcgtcca cgttcgccag tgactttctg
    52321 tagctcttcc ggacgcagtt taattttgcg tttcccaaca tgaatggtca gcgtgctttg
    52381 cggcggcaga acgtacagtt gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg
    52441 aatgttgata atcttgttgc ctttgccctt cgacagctgc ggcagatcgc ttaccgggaa
    52501 catcaacata cggcctgcct gagtgattgc cagcagcata tcggaagcat cttcaatcac
    52561 caccggcggc ataacatggg cattttccgg taaggtgatc aaagccttac ccgcacggtt
    52621 acgcgctacc agatcgttaa aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag
    52681 cagtttctga tcgtcgcttt ccatcagcat atggtcaacg gtcgccccag gcggcaacgt
    52741 taatttgccg gtgagtggtt cgccctgacc acgcgccgac ggcagcgtaa tcgggtcgat
    52801 ggcatagcta cgaccggtgg aatcaacaaa cactaccggt tggttactct tacctttcac
    52861 cgccgctttg aagctatcac ccgctttata attcaggccc ggcgcgtcga tatcatggcc
    52921 tttagcgctg cgtacccagc ccatctgcga cagcacaatg gtgacaggtt cagacggcag
    52981 catgtcgtgc tcgctcatcg ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc
    53041 gtcaccgtag gcttgcgcgt ctgcctgcag ttctttcttc agcaggttat tcattttacg
    53101 ctcggaagcc aaaatgccct gcaactggtc gcgctctttt tccagctcac tctgctcacc
    53161 gcgaatcttc atctcttcca gtttggcaag atgacgcagt ttcagttcga ggatcgcttc
    53221 cgcctgggtt tccgtaaggc caaaccgcga catcagcgcc ggtttcggtt catcttcgtt
    53281 acgaatgatc tcaatcactt cgtcgatgtt gagaaacgcc accagcaaac cttccaggat
    53341 atgcaggcgc ttgaggactt tctccagacg atagttcagt cggcggcgca cggtatcgcg
    53401 acggaacacc agccattcgg agaggatttc cagcaggttt ttcaccgccg gacgaccatc
    53461 cagaccgatc atattgaggt taatgcgata gctcttttcc agatcggtgg tagcgaagag
    53521 gtggttcatc acctgatcca tatccacgcg gttggaacgc ggcacaatca ccagacgggt
    53581 cgggttctcg tggtcagatt catcgcgcag atcgtcaacc atcggcagct ttttgttgcg
    53641 catttgcgca gcaatttgct ccagtacgcg cgcacctgaa acctgatgcg gtaatgcgct
    53701 gataaccacc gcgccatctt ctttcttcca caccgcgcgc atacgcactg aaccacgtcc
    53761 gttctcgtag attttacgaa tctcggcgcg cgaagtgata atttccgctt cagtcggata
    53821 atccggcccc tgcacgatat ccagcagctg atcgagcgta gttttcggct ggtcgattaa
    53881 tgcgattgcc gcctgagcta cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc
    53941 gacggcaata ccggtggtgc cgttaagcaa aatgtttggc agacgggcag gtagcatttt
    54001 cggctcctgc aaagtgccgt cgaagtttgg cacccagtca gccgtcccct gccccagctc
    54061 gctcaatagc agctcggaat atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa
    54121 cgatttcgga tcgtccggcg cgccccagtt cccctgacca tcaaccagcg gataacggta
    54181 agagaacggc tgcgccatca ggaccatcgc ttcataacag gcgatatcgc cgtgcggatg
    54241 gtatttaccc agtacgtcac cgacggtacg ggccgatttt ttaaatttgg cgctggcatt
    54301 caggcccagt tcagacatcg catacacaat gcggcgctga acaggtttca gaccatcacc
    54361 aataaacggc aacgcacggt ccatgatcac gtacatggag tagtttaagt aggcgttttc
    54421 cgtaaattca tgtagcgcaa ggcgctctgc catatcgctc attaattctg attcctcaac
    54481 ttattcgcca gccttcaaag gggcaatatt gccggagata ctacattatc tgccgcgtcg
    54541 agtcacagga gatcagcgtg aatagcgccg ccggacgtgc tccgacggcg ataaaggcat
    54601 caatgtgcct tgatatacaa ctctttgctg tagacttgat cggcatagag ggcagcacaa
    54661 ggttgcgccc atgccacacc actcggcatg ggggtccgct ccgggcgatt cgagaagttg
    54721 aggttatgcg agtccaggga agcctaaagc atcaaagtag tcgactgtaa gtatacggtt
    54781 cagcccagtt tagctgtgtc tccaccagtg ccccgcgcct cctgccataa gtgcggcttg
    54841 ctcatggata tccccgagac ggcttacggc gcaccggata ataaatttcg cgtatacatg
    54901 gtattcctta ccggagaaat tataagccca acaacaatac gcctgtgaca ttgttcgctc
    54961 aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttccccatca cgccaacggc
    55021 acataaacat ccgtttccag tcgctgctct gtcacccctt cggcaagatt ggtgtagtga
    55081 aacagaatcg gtgctttacg cattttctcg ccgcttgcag gcagccagtg gcgaataatg
    55141 ccccatatcg tgtgtgaaat atcgtccagc tcgccaacgt ggcgggccac ggcataacgt
    55201 ccaccggtaa gttcaccatt gctcacacca taacgattat cgggaatcgg ttcgctaacg
    55261 ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg tggcaggggc gtcccaggcg
    55321 acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata
    55381 aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca
    55441 gggattgatg ggaaatcgat aatgtttaca tccagtgtca ggtttgtcat tttgcctccc
    55501 tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac aattacggat ttactttgcg
    55561 gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc
    55621 aaccgtatct ttcggcgtca ccgtcacacc gttccagcgt ttgtgatcga tatcaacatt
    55681 gatagtaccg ctggcatctt taaacacgta gagatcgtca gagatgcgtt caacgatatt
    55741 gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta cagtcgttac
    55801 gctgccgttc ggcccctgaa atcctccggc ctgactttgc gttaccgatg ggccagaaaa
    55861 accgccctgc tctgctgcca tcaccggtgc actgcacagg gccattactg cgattactgc
    55921 tgcgaatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat
    55981 agtccttaac aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac
    56041 aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact
    56101 gatagaagat gacatgctga ttggtgacgg catcaaaact ggccttagta aaatgggttt
    56161 tagtgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctttata gcgcacctta
    56221 tgatgcggtg atcctggatt taaccttacc aggcatggat ggtcgcgata ttttgcgcga
    56281 atggcgagag aaaggtcagc gtgagccggt actgaacctg accgcgcgtg atgcgttagc
    56341 ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt
    56401 gatagaagtc gccgccaggc tggaggctct gatgcgccga accaacggcc aggccagcaa
    56461 cgagctgcgc cacggtaacg tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg
    56521 cgaaccctta acactgaaac caaaagaatt tgccctgctg gaattactga tgcgtaacgc
    56581 tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt
    56641 caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gtagtgattt
    56701 tattcgtacc gtgcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt
    56761 cttagtctgc gcgtcaggct gacgctaatc tttttaattc tggcctcggt gacctggctg
    56821 ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgaatt gttcgacacc
    56881 caactgatgc tgtttgccaa acggttaagt acgctcgatc tcaacgaaat caacgcggcg
    56941 gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga cgatgcgctg
    57001 acctttgcca tctttaccca cgacggcaga atggtcctta atgatggcga taacggagaa
    57061 gatattccct atagctatca acgggaaggt tttgctgacg ggcaactggt cggtgaagac
    57121 gatccttggc gttttgtctg gatgacctca cctgatggca aatatcgcat cgttgttggc
    57181 caggaatggg aataccgtga agacatggcg ctggcgattg ttgccgggca attgatcccg
    57241 tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc
    57301 gcgccgctga acaaactggc gctggcacta cgtatgcgtg accctgactc ggaaaaacca
    57361 ctaaacgcga ctggcgtacc cagcgaagtg cggccactgg ttgagtcgct aaatcaactg
    57421 ttcgcccgca cacatgcgat gatggttcga gaacgacgct ttacctccga tgcagctcac
    57481 gaactacgta gcccgttaac agcgctgaaa gtgcaaaccg aagttgcgca gctctctgac
    57541 gatgatccgc aggcgcggaa aaaagcactg ctccaattac attccgggat cgatcgcgct
    57601 actcgtctgg ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag
    57661 gacgtcgcgg agatcccgct tgaagatctc ctacaatcgt cggtgatgga tatttaccac
    57721 acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgcccacgg catcaaacgc
    57781 accggacaac cgctattgct aagtttgttg gtgcgaaatt tgctggataa cggccccggt
    57841 gtgacaccag aggcactggc gcgaattggc gaacgcttct atcgcccacc cggacaaacc
    57901 gctaccggca gtggacttgg gctatcgatt gtccagcgaa tcgccaagct gcatgacatg
    57961 aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt
    58021 ctctgtctat gagagccgtt aaaacgactc tcatagattt tactaatagc aaaatataaa
    58081 ccgtccccaa aaaagccacc aaccacaaac cagacaaata acgagcgaga atacatatag
    58141 ccaaaactcg aacatttccc gcataaagag ttttcttaag ataagaataa taagtggcgt
    58201 aagaagaaaa aatgctgcaa tcattgcttg caatatacga tcttcgcctg aaacggaagg
    58261 tatactgcaa atattgcctc ccgactcaag catccagtca tacttattga aagcaaaaaa
    58321 agaatctatt aaccaataag taagcattat atatggcaat gcttcaaata ttgttttaat
    58381 tttctgcttt aacatatatc agtacgctca taaaaatcca tttataatta tcaacatgac
    58441 acgttgatat tttcatcttt tacttattca aagccatatt ttcaatcccc aaaaaaaccg
    58501 aaccaaacgt gtaaattatt ccggtgcgag taagaattat gaggaatggc tatcagtatt
    58561 gtcattttca gaaaatattt atcctgcatc ggtgagtcag agctagatca gacttttgct
    58621 aaattcgcaa aagactttgc acattttgct aatttcacag taccgctctg tgacgtacta
    58681 tagtcgacaa acgtctcacc ttgagattaa aaaatgagca acatcctgat tatcaacggc
    58741 gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga agtcgcggat
    58801 ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc gcgccgacag cgactacaat
    58861 gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca gatgccaggc
    58921 tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt caccgaaggt
    58981 cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccgtcgaa aaaatacggt
    59041 tccggcagcc tggtacagga caaaaaatat atgctttctc taacctggaa cgcaccaatg
    59101 gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gcgttgacgg tgtgtatctg
    59161 ccgttccata aagcaaacca attcctcggt atggaaccgc tgccgacatt tatcgctaat
    59221 gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa gcatcttgtg
    59281 gaaatttttg gttaactaga gctcaggctt tagaaggagt taaccatgct taccgtaatc
    59341 gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cggtattgga tcagtttgct
    59401 aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc aatggtggat
    59461 tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat gattgagcag
    59521 tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc cgcacatgaa ggcgtacagc
    59581 gaagccgtaa aaggcgacgt gctggagatg aatatccgta ttctgcagcc agggatttaa
    59641 tcctgccttg tttgcccggc catcctgacc gggcaatgtt ctttccttta aacctcaatc
    59701 tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc
    59761 agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc
    59821 accagacggc gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg gttcatttcc
    59881 cccagacctt taaaacgctg gacgttcggc ttgcctttct tgcgttttaa ttgctcaagt
    59941 acgccctctt tctcttcttc cgtcagcgcg taataaacct ctttcccgag atcaatacgg
    60001 tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg gaagtgtttt
    60061 acgaacaaag cgcagagcag cgtggcaatg tgcagaccat cagagtccgc atccgcgagg
    60121 atacagattt tgccataacg aagctggctc agatcgtcgc tgtcaggatc gataccgatc
    60181 gctaccgaaa tatcatgcac ttcctgcgag gccagcactt cgtcggaaga aacttcccag
    60241 gtgttaagga tcttaccttt cagcggcatg atcgcctgat attcacgatc gcgcgcctgc
    60301 ttggcagatc cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg gttaaggccc
    60361 tgcgcggtac aatcagccag tttgccaggc agcgccgggc cgctggtcag ctttttacgc
    60421 accacttttt tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccagc
    60481 aactccgccg cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc
    60541 gaaacgaatg ccgcacactg acgcgaagag agacgctctt tggtctgccc ggcaaactgc
    60601 ggatcctgca ttttaaccga cagaacatag gcgcagcgat cccagatatc ttccgccgac
    60661 agcttcacgc cgcgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccaac
    60721 aggccctgac gcagaccatt aacatgggta ccgccctgca tcgttgggat aaggttgacg
    60781 tagctttcgg tcagcagttc accgccttcc ggcagccaca gtagcgccca gtccacagct
    60841 tcagtatcgc cagcgaaatt accgataaac ggtttttctg gcagcgtcgg taaaccgttt
    60901 accgcttccg ccaggtaatc attcagaccg tcctgatagc accagcgctg ttcggtgttg
    60961 ttgatctcat ctttaaaggt aatctcaacg ccggggcaca gtactgcttt ggccttcagc
    61021 acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa agaaggtttc atccggccag
    61081 aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc cgacaacctg taaatcctgc
    61141 accttttcgc cattttcaaa ggcgatgtta taaacctgac catcgcggcg cacgttaact
    61201 tctacgcgct tcgacaaggc gttaactacc gaaatcccca cgccatgcag gccgccagag
    61261 aactggtaat ttttgttaga gaatttaccg cctgcatgca gacggcaaag aatcagttca
    61321 accgccggta ctccttcttc cgggtgaata tccaccggca tcccgcgccc atcgtcaatc
    61381 acttctaacg actggtcagc atgtaagatt acatccacgc gttttgcgtg acccgccagt
    61441 gcttcatcga cactgttatc aataacttct tgccccaaat ggttagggcg agtggtatcg
    61501 gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc aatggcatca
    61561 gcgttataag tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc agagatggtg
    61621 cagaccaaga aaatcgacga tcgggttgaa ataatcttcg aagcccgtga atgcgtggtt
    61681 gccgccttct atgacagtct ggcggcagga agcatagtac gccaccgcct ggcggtaatc
    61741 cagcacttca tctcccgtct gttgcagcag ccagatcaaa tccggcgctt ccagcgggtc
    61801 aatctgcatg actttgagat cgtaaatatg gcgtgactct agcacatatt gctgcccggt
    61861 gtaggggttc tcgttctgac cgagatagtc cgtcagcaat tcaaacgggc gtaccgccgg
    61921 gtttaccacc actgcgggca gcataaaaca ttgcgacaac caggtggcgt aatatccccc
    61981 cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt tccaggacaa tggattccag
    62041 cagttctgcc gcgtcggaag gatacggcgg caactgcgga atgatcatct caacgtcagg
    62101 gtgatgttcc gccagccagt tttttaacaa gctcgctttt gcagagcgcg gcgagctgtt
    62161 gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt ctgaagcggt atcaggttgg
    62221 aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc atgtaactcg
    62281 agagtacgcc agccgggcgc gatagtatcc agcgtaaagt tggaacagtg cggcttaaac
    62341 tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag atccagctcc
    62401 tgatgaatat gaccgcacag caagtatttg acgtgcggaa acttcgccag cacggtatcc
    62461 agttcgcccg cgttacgcag actgtgttga tcgagccaac tacaacccgc aggtagcgga
    62521 tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat cggccagttt acgttccagc
    62581 cactcaagct gaaactcgct cagctcaccg tgcggcacgc caaacacctg gctatccagc
    62641 aacaggattt gccattgctc accaataaac acccgcttcg ccggggagat acccgcatcc
    62701 tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca gacgcagggc
    62761 gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga ttgatcctgc
    62821 gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct ggtgtggacg aatcgcctcc
    62881 agcaccgcct ggtagctttc ccaggtgttt acccctaaca gggcttcgtg cttttgtgca
    62941 aacaggtgag tatcggtaat ttgtaaaatc cttcctctgg cctcaccagc cagaggaagg
    63001 gttaacaggc tttccaaatg gtgtccttag gtttcacgac gctagtaaac cggaatcgcc
    63061 atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactggtt aatttgatgc
    63121 ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt gaagcgaaaa
    63181 atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg caccgtcatt
    63241 gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac cagggtagtg
    63301 tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg atagcttaca
    63361 gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa gttcatctcg
    63421 cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc ccactcattt
    63481 tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc tgcgttgtcg
    63541 attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacatg aacgcgaata
    63601 tcttcgtttt catcagccag accatgaata ccgcttgcgg tcgtggcgtc cacttcgccc
    63661 accataattg acgaacgctc actggtgccc cccgggcttg ccaggaaact taacaccggt
    63721 ttggtccgtt tgactatcag tcccgcctct tcaatcgctt cgcgacgggc aacatcttcc
    63781 acactttcac cctcttcaat catcccggca accatctcca gtagccaggg ggtttcgctg
    63841 gtgtcgtacg cggcaatccg aatctgctca atcagcacaa cttcatcacg cactgggtca
    63901 aagggtagca agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac ctcatgactc
    63961 atttgcccgt tgaatagacg atgacgaaat ctataaagat ctaatgaaaa aaagccgcga
    64021 taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac gggcaggttg
    64081 tctggcttaa gcattgttaa tgtcctagca ctaatagtga attaaatgtg aatttcagcg
    64141 acgtttgact gccgtttgag cagtcatatg ttaaattgag gcacattaac gccctatggc
    64201 acgtaacgcc aaccttttgc ggtagcggct tctgctagaa tccgcaataa ttttacagtt
    64261 tgatcgcgct aaatactgct tcaccacaag gaatgcaaat gaagaaattg ctccccattc
    64321 ttatcggcct gagcctttct gggttcagtt cgttgagcca ggccgagaac ctgatgcaag
    64381 tttatcagca agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc gatcgtgatg
    64441 ctgcctttga aaaaattaat gaagcgcgca gtccattact gccacagcta ggtttaggtg
    64501 cagattacac ctatagcaac ggctaccgcg acgcgaacgg catcaactcg aacgcgacca
    64561 gtgcgtccct gcagttaact caatccattt ttgatatgtc gaaatggcgt gcgttaacgc
    64621 tgcaggaaaa agcagcaggg attcaggacg tcacatatca gaccgatcag caaaccttga
    64681 tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt ctttcctata
    64741 cacaggcaca aaaagaagcg atctaccgtc aattagatca aaccacccaa cgttttaacg
    64801 tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat accgtgctgg
    64861 cgaacgaagt gaccgcacgt aataaccttg ataacgcggt agagcagctg cgccagatca
    64921 ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa accgacaaac
    64981 cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg ctgttacagg
    65041 cacgcttgag ccaggacctg gagcgcgagc aaattcgcca ggcgcaggat ggtcacttac
    65101 cgactctgga tttaacggct tctaccggga tttctgacac ctcttatagc ggttcgaaaa
    65161 cccgtggtgc cgctggtacc cagtatgacg atagcaatat gggccagaac aaagttggcc
    65221 tgagcttctc gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg aaacaggcac
    65281 agtacaactt tgttggtgcc agtgagcaac tggaaagcgc acatcgtagc gtcgtgcaaa
    65341 ccgtacgttc ctccttcaac aacattaatg cttctatcag tagtattaac gcctacaaac
    65401 aagccgtagt ttccgcgcaa agctcattag acgcgatgga agcgggctac tcggtcggta
    65461 cgcgtaccat tgttgatgtg ttggatgcga ccaccacgct gtacaacgcc aagcaagagc
    65521 tggcgaatgc gcgttataac tacctgatta atcagctgaa tattaagtca gccctgggta
    65581 cgttgaacga gcaggatttg ctggcactga acaatgcgct gagcaaaccg gtttccacta
    65641 atccggaaaa cgttgccccg caaacgccgg aacagaatgc tattgctgat ggttatgcgc
    65701 ctgatagccc ggcacccgtc gttcagcaaa catccgcacg cactaccacc agtaacggtc
    65761 ataacccttt ccgtaactga tgacgacgac ggggcttcgg ccccgtctga acgtaaggca
    65821 acgtaaagat acgggttatc tgccgcattc ttcccccttc tcgcttcaat ttcgaccagc
    65881 catcctctat tctgatgggt atttaccact ggtcccggaa gacaaaaatg aaacggacaa
    65941 aatccatacg ccacgcatcg ttccgcaaaa actggagcgc acgccatctg acaccagtcg
    66001 ctctcgcggt tgccactgtt cttatgctgg ctggctgtga aaagagtgat gaaacagtgt
    66061 ctctctatca aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc gccgaatgta
    66121 ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa tacgccaccc
    66181 gtgaagactg tgttgctgaa tttggtgaag gtcagtgtca gcaagcacca gcccaggctg
    66241 gcatggcacc agaaaaccag gcgcaggctc agcaatccag cgggagtttc tggatgccgc
    66301 tgatggccgg ttacatgatg gggcgtctga tgggcggcgg cgcgggattt gcacagcagc
    66361 cgctgttctc ctcgaaaaac ccggccagtc cggcttacgg taaatatacc gacgcgacgg
    66421 gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag acggcaatgg
    66481 caccaaaacc ggcgaccacc actaccgtta cccgtggcgg ttttggtgaa tctgttgcca
    66541 aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg ggtggctgat
    66601 accgatggaa agagtcagta ttaccgagcg cccggactgg cgtgagaaag cccacgaata
    66661 cggtttcaat tttcacacca tgtacggcga gccgtactgg tgtgaagatg cttactacaa
    66721 gttgaccctc gcccaggttg aaaagctgga agaagtcacc gccgaactgc accagatgtg
    66781 cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg atgaccaaat tccgcatacc
    66841 aaaacacacc tggagttttg tgcgtcaatc atggctgact caccagccat cgctttattc
    66901 gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa cttctggaaa ataacgccga
    66961 tacgccaacg tcactatacg aggcggcgtt ctttcagtgg atctggctgg aagatcagct
    67021 taacgccggt aacttgccgg agggcagcga ccagtttaac agtctgcaag aaaaactgat
    67081 cgatcgcttc gttgagctgc gtgaacagta tggcttccag ttgctgcatc tcacctgctg
    67141 tcgcgacacg gtggaagatc gcggaaccat tcagtatttg caggactgcg caaccgaagc
    67201 tgaaatcgcc accgagttcc tctacatcga cgagataggt ttaggcgaaa aaggccagtt
    67261 cacggattta caggatcagg tgatttctaa cctgttcaag ctttatccgt gggaatttat
    67321 gctgcgcgaa atgttctcca ccaagctgga agatgctggc gtacgctggc tggaaccggc
    67381 gtggaagagc attatctcca acaaggcact tctaccgcta ctgtgggaga tgttcccgaa
    67441 tcacccgaac ctgctgcccg cttattttgc ggaagatgat catccgcaaa tggaaaaata
    67501 tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg agaacggcaa
    67561 aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg atgattgttc agcaattcca
    67621 cccgttaccg aaattcggcg acagctatat gctgattggt agctggctgg tgaacgatca
    67681 acctgccgga attggcattc gtgaagaccg tgcgttgatc acccaggata tgtctcggtt
    67741 ttatccacat atttttgttg aataagccac gataccggat ggcactcgcc atccggtaat
    67801 tgttagccta tctgcaccga cagcatactt aaactgccca tttctatacc atcgaccgga
    67861 atggtaattg gctcctgccc atcccacaca cctaacacat acaacaacgg cagatagtgc
    67921 tctggcgttg ggttcgataa cgcgccacct tcatggtcga gataattcac cagaggatgc
    67981 tgctctaccg gcccttgcca cgtcagattc gctttcacat actcattaaa cgacgtcgcc
    68041 cacggatacg gtgaactatc accgtgccac ttcactgtgc gcaggttatg caccacgtta
    68101 ccgctggcga ccaacattat tccttcatca cgcagcgccg cgagtttgcg ccccatttcg
    68161 aaatgccagg cggcaggttt gctactgtcg atactcaact gcaccatagg gatatcggcg
    68221 tcaggataca tcttaatcag cacgccccac gagccgtggt caaagcccca ggcttcttta
    68281 tccagcgtca ccgggatcgg cgctaacagc tcaaccagac gctgtgccag cgcaggcgaa
    68341 cccggagcag gataatgcgt atcgtacagc gcctgcggga agccaccaaa gtcatgaatc
    68401 gtgggcggcg tctccatcgc ggtcactcct gttccacggg taaaccagtg agccgaaacc
    68461 accacaatcg cttgcgggcg tggcaatgtc atccccaact tctgccagct gcgggtatac
    68521 aaattatctt ccagcacgtt catcggacta ccgtgaccta aaaacaatgc tggcatacgt
    68581 gttgaagaca tgatgatatc cttaactaaa ggtgtcattt tgatatcctc acaatacgct
    68641 tgttcggcgg agtaagaacc cggataacaa tgatgatgat catcagttat tttgacgatc
    68701 tgcctgaagg tgaagattta taaggagttg tcgatgtcag tacctctcat tctgaccata
    68761 ctggcggggg cagccacgtt tattggcgcg tttctcggcg ttctcggaca aaaaccctcg
    68821 aaccgcttac tggcgttttc gctaggtttt gcggcgggga tcatgttgct catctcatta
    68881 atggaaatgc ttcctgccgc actagcagct gaaggaatgt cgccggtgtt gggttatgga
    68941 atgtttatct tcggtctgct tggctacttt ggcctggacc gcatgttgcc gcatgcgcat
    69001 ccgcaggatt taatgcaaaa atcggtgcag ctgttgccaa aatcgatcaa gcgcacagcc
    69061 attctgctca ctctcggcat cagtctgcat aacttcccgg aagggattgc cacctttgtc
    69121 acggcgagca gcaacctgga gctgggattt ggcatcgcac tggccgtcgc gttgcacaat
    69181 atccctgaag gtctggcagt ggcaggcccg gtttatgcgg caacggggtc taaacgcacc
    69241 gcgattctgt gggcggggat ttctggacta gcagaaattc ttggtggtgt gctggcgtgg
    69301 ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg caatcatggc ggcggttgca
    69361 ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga gattgaccct
    69421 aataataacc ccagctatgg cgtactatgt ggaatgtcag tgatgggatt cagtttagtg
    69481 ctgctacaaa cggcgggaat tggttaaaaa caacatcaga agttgtccag cacatggaca
    69541 acttctgcca gcttcacgaa agccttattc taccttttcg gctgtctctt ctctcgtact
    69601 gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa acgcccgaca
    69661 ataacgtttc aaatgcagga aataacccac cacaaccaga gcaatcaata caatccagta
    69721 ccaggcgata aacatccttt tacccttaat atttaacaat actaaacatt tagcgcataa
    69781 atttcacata tcctttttcg gatatattca tatggtcgca taataacgga acaattatat
    69841 gattttgatc ataatgaaaa agaatgcctg atattaatga aattttctca tagaaagaga
    69901 agcggaatta acatcttgca tagcagggca aaataaaaac cgaagccagc tacaacttcg
    69961 gtttcttgat taaggcagta attaagcagc ggttttcagc tggctttacg ctcatgtgcc
    70021 tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt attggcaaac
    70081 tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc acacagcaca
    70141 ccagccggtt taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt atgaccgcca
    70201 cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg gcgattcaga
    70261 tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg atcagcggca
    70321 gaaacaccgg tagtcacacc ttcagccgct tcaatggtca cggtaaaacc ggtgccatag
    70381 gcgctggtgt tattttctac catcattggc agatcaagtt gtttacggcg atcttcagtg
    70441 atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg ctcaacggtc
    70501 atggtttctg ccgggaagat catatcgcct tcgttttcac ggtcttcatc atcaagcacc
    70561 attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc gaaaggcgta
    70621 ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaaa cctcactaaa attatggtta
    70681 ccagaatcag ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca
    70741 tgcccgacag aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca
    70801 ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga
    70861 tttaccgccg gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta
    70921 atgattagcg gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg
    70981 cactacaatg gtactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa
    71041 aattgagcaa atcgctcgcc aggttcacga atcaatgcct aaaggaatca
//
LOCUS       DAJTYH010000004        70046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTYH010000004 DAJTYH010000000
VERSION     DAJTYH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..796
                     /locus_tag="OO609_000260"
     CDS             <1..796
                     /locus_tag="OO609_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6631808.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            807..1898
                     /locus_tag="OO609_000261"
     CDS             807..1898
                     /locus_tag="OO609_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6631809.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1941..2801)
                     /gene="rsmI"
                     /locus_tag="OO609_000262"
     CDS             complement(1941..2801)
                     /gene="rsmI"
                     /locus_tag="OO609_000262"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6631810.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2866..4902
                     /gene="lpoA"
                     /locus_tag="OO609_000263"
     CDS             2866..4902
                     /gene="lpoA"
                     /locus_tag="OO609_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6631811.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4860..5255
                     /locus_tag="OO609_000264"
     CDS             4860..5255
                     /locus_tag="OO609_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6631812.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5275..5865
                     /gene="diaA"
                     /locus_tag="OO609_000265"
     CDS             5275..5865
                     /gene="diaA"
                     /locus_tag="OO609_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6631813.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5875..6450
                     /gene="yraP"
                     /locus_tag="OO609_000266"
     CDS             5875..6450
                     /gene="yraP"
                     /locus_tag="OO609_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6631814.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6564..7604)
                     /locus_tag="OO609_000267"
     CDS             complement(6564..7604)
                     /locus_tag="OO609_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6631815.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7677..8312)
                     /locus_tag="OO609_000268"
     CDS             complement(7677..8312)
                     /locus_tag="OO609_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6631816.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8440..8958
                     /gene="yhbO"
                     /locus_tag="OO609_000269"
     CDS             8440..8958
                     /gene="yhbO"
                     /locus_tag="OO609_000269"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6631817.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8938..9381)
                     /locus_tag="OO609_000270"
     CDS             complement(8938..9381)
                     /locus_tag="OO609_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6631818.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9432..9734
                     /gene="yhbQ"
                     /locus_tag="OO609_000271"
     CDS             9432..9734
                     /gene="yhbQ"
                     /locus_tag="OO609_000271"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6631819.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9721..10224)
                     /locus_tag="OO609_000272"
     CDS             complement(9721..10224)
                     /locus_tag="OO609_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6631820.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10218..10742)
                     /locus_tag="OO609_000273"
     CDS             complement(10218..10742)
                     /locus_tag="OO609_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6631821.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10951..11946
                     /locus_tag="OO609_000274"
     CDS             10951..11946
                     /locus_tag="OO609_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6631822.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11955..12833
                     /locus_tag="OO609_000275"
     CDS             11955..12833
                     /locus_tag="OO609_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6631823.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12914..13921
                     /locus_tag="OO609_000276"
     CDS             12914..13921
                     /locus_tag="OO609_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6631824.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14039..15283)
                     /gene="mtr"
                     /locus_tag="OO609_000277"
     CDS             complement(14039..15283)
                     /gene="mtr"
                     /locus_tag="OO609_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6631825.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15437..17326)
                     /gene="deaD"
                     /locus_tag="OO609_000278"
     CDS             complement(15437..17326)
                     /gene="deaD"
                     /locus_tag="OO609_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6631826.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17319..17399)
                     /gene="yrbN"
                     /locus_tag="OO609_000279"
     CDS             complement(17319..17399)
                     /gene="yrbN"
                     /locus_tag="OO609_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6631827.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17506..18390)
                     /gene="nlpI"
                     /locus_tag="OO609_000280"
     CDS             complement(17506..18390)
                     /gene="nlpI"
                     /locus_tag="OO609_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6631828.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18499..20634)
                     /gene="pnp"
                     /locus_tag="OO609_000281"
     CDS             complement(18499..20634)
                     /gene="pnp"
                     /locus_tag="OO609_000281"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6631829.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20881..21150)
                     /gene="rpsO"
                     /locus_tag="OO609_000282"
     CDS             complement(20881..21150)
                     /gene="rpsO"
                     /locus_tag="OO609_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6631830.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21299..22243)
                     /gene="truB"
                     /locus_tag="OO609_000283"
     CDS             complement(21299..22243)
                     /gene="truB"
                     /locus_tag="OO609_000283"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6631831.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22243..22644)
                     /gene="rbfA"
                     /locus_tag="OO609_000284"
     CDS             complement(22243..22644)
                     /gene="rbfA"
                     /locus_tag="OO609_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6631832.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22808..25456)
                     /gene="infB"
                     /locus_tag="OO609_000285"
     CDS             complement(22808..25456)
                     /gene="infB"
                     /locus_tag="OO609_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6631833.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25481..26968)
                     /gene="nusA"
                     /locus_tag="OO609_000286"
     CDS             complement(25481..26968)
                     /gene="nusA"
                     /locus_tag="OO609_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6631834.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26996..27448)
                     /gene="rimP"
                     /locus_tag="OO609_000287"
     CDS             complement(26996..27448)
                     /gene="rimP"
                     /locus_tag="OO609_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6631835.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27655..27731)
                     /locus_tag="OO609_000288"
     tRNA            complement(27655..27731)
                     /locus_tag="OO609_000288"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27695..27697),aa:Met,seq:cat)
     gene            28079..29422
                     /gene="argG"
                     /locus_tag="OO609_000289"
     CDS             28079..29422
                     /gene="argG"
                     /locus_tag="OO609_000289"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6631836.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29430..31037)
                     /locus_tag="OO609_000290"
                     /pseudo
     CDS             complement(29430..31037)
                     /locus_tag="OO609_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31515..31601)
                     /locus_tag="OO609_000291"
     tRNA            complement(31515..31601)
                     /locus_tag="OO609_000291"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31565..31567),aa:Leu,seq:gag)
     gene            complement(31616..31948)
                     /gene="secG"
                     /locus_tag="OO609_000292"
     CDS             complement(31616..31948)
                     /gene="secG"
                     /locus_tag="OO609_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6631837.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32176..33513)
                     /gene="glmM"
                     /locus_tag="OO609_000293"
     CDS             complement(32176..33513)
                     /gene="glmM"
                     /locus_tag="OO609_000293"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6631838.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33506..34354)
                     /gene="folP"
                     /locus_tag="OO609_000294"
     CDS             complement(33506..34354)
                     /gene="folP"
                     /locus_tag="OO609_000294"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6631839.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34444..36378)
                     /gene="ftsH"
                     /locus_tag="OO609_000295"
     CDS             complement(34444..36378)
                     /gene="ftsH"
                     /locus_tag="OO609_000295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6631840.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36478..37107)
                     /gene="rlmE"
                     /locus_tag="OO609_000296"
     CDS             complement(36478..37107)
                     /gene="rlmE"
                     /locus_tag="OO609_000296"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6631841.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37233..37526
                     /gene="yhbY"
                     /locus_tag="OO609_000297"
     CDS             37233..37526
                     /gene="yhbY"
                     /locus_tag="OO609_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6631842.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37682..38158)
                     /gene="greA"
                     /locus_tag="OO609_000298"
     CDS             complement(37682..38158)
                     /gene="greA"
                     /locus_tag="OO609_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6631843.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38406..39839
                     /gene="dacB"
                     /locus_tag="OO609_000299"
     CDS             38406..39839
                     /gene="dacB"
                     /locus_tag="OO609_000299"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6631844.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40025..41197)
                     /gene="cgtA"
                     /locus_tag="OO609_000300"
     CDS             complement(40025..41197)
                     /gene="cgtA"
                     /locus_tag="OO609_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6631845.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41213..42178)
                     /locus_tag="OO609_000301"
     CDS             complement(41213..42178)
                     /locus_tag="OO609_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6631846.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42305..42562)
                     /gene="rpmA"
                     /locus_tag="OO609_000302"
     CDS             complement(42305..42562)
                     /gene="rpmA"
                     /locus_tag="OO609_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6631847.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42583..42894)
                     /gene="rplU"
                     /locus_tag="OO609_000303"
     CDS             complement(42583..42894)
                     /gene="rplU"
                     /locus_tag="OO609_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6631848.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43152..44123
                     /gene="ispB"
                     /locus_tag="OO609_000304"
     CDS             43152..44123
                     /gene="ispB"
                     /locus_tag="OO609_000304"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6631849.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44350..44628
                     /gene="sfsB"
                     /locus_tag="OO609_000305"
     CDS             44350..44628
                     /gene="sfsB"
                     /locus_tag="OO609_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6631850.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44676..45935)
                     /gene="murA"
                     /locus_tag="OO609_000306"
     CDS             complement(44676..45935)
                     /gene="murA"
                     /locus_tag="OO609_000306"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6631851.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45990..46244)
                     /gene="ibaG"
                     /locus_tag="OO609_000307"
     CDS             complement(45990..46244)
                     /gene="ibaG"
                     /locus_tag="OO609_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6631852.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46404..46697)
                     /gene="mlaB"
                     /locus_tag="OO609_000308"
     CDS             complement(46404..46697)
                     /gene="mlaB"
                     /locus_tag="OO609_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6631853.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46697..47332)
                     /gene="mlaC"
                     /locus_tag="OO609_000309"
     CDS             complement(46697..47332)
                     /gene="mlaC"
                     /locus_tag="OO609_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6631854.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47351..47902)
                     /gene="mlaD"
                     /locus_tag="OO609_000310"
     CDS             complement(47351..47902)
                     /gene="mlaD"
                     /locus_tag="OO609_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6631855.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47907..48689)
                     /gene="mlaE"
                     /locus_tag="OO609_000311"
     CDS             complement(47907..48689)
                     /gene="mlaE"
                     /locus_tag="OO609_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6631856.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48697..49506)
                     /gene="mlaF"
                     /locus_tag="OO609_000312"
     CDS             complement(48697..49506)
                     /gene="mlaF"
                     /locus_tag="OO609_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6631857.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49716..50681
                     /locus_tag="OO609_000313"
     CDS             49716..50681
                     /locus_tag="OO609_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6631858.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50695..51681
                     /gene="kdsD"
                     /locus_tag="OO609_000314"
     CDS             50695..51681
                     /gene="kdsD"
                     /locus_tag="OO609_000314"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6631859.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51702..52268
                     /gene="kdsC"
                     /locus_tag="OO609_000315"
     CDS             51702..52268
                     /gene="kdsC"
                     /locus_tag="OO609_000315"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6631860.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52265..52840
                     /gene="lptC"
                     /locus_tag="OO609_000316"
     CDS             52265..52840
                     /gene="lptC"
                     /locus_tag="OO609_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6631861.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52809..53366
                     /gene="lptA"
                     /locus_tag="OO609_000317"
     CDS             52809..53366
                     /gene="lptA"
                     /locus_tag="OO609_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6631862.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53373..54098
                     /gene="lptB"
                     /locus_tag="OO609_000318"
     CDS             53373..54098
                     /gene="lptB"
                     /locus_tag="OO609_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6631863.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54146..55579
                     /gene="rpoN"
                     /locus_tag="OO609_000319"
     CDS             54146..55579
                     /gene="rpoN"
                     /locus_tag="OO609_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6631864.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55602..55889
                     /gene="hpf"
                     /locus_tag="OO609_000320"
     CDS             55602..55889
                     /gene="hpf"
                     /locus_tag="OO609_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6631865.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56007..56498
                     /gene="ptsN"
                     /locus_tag="OO609_000321"
     CDS             56007..56498
                     /gene="ptsN"
                     /locus_tag="OO609_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6631866.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56544..57398
                     /gene="rapZ"
                     /locus_tag="OO609_000322"
     CDS             56544..57398
                     /gene="rapZ"
                     /locus_tag="OO609_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6631867.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57395..57667
                     /gene="npr"
                     /locus_tag="OO609_000323"
     CDS             57395..57667
                     /gene="npr"
                     /locus_tag="OO609_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6631868.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57880..58512
                     /gene="yrbL"
                     /locus_tag="OO609_000324"
     CDS             57880..58512
                     /gene="yrbL"
                     /locus_tag="OO609_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6631869.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58509..59236)
                     /gene="mtgA"
                     /locus_tag="OO609_000325"
                     /pseudo
     CDS             complement(58509..59236)
                     /gene="mtgA"
                     /locus_tag="OO609_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59233..59886)
                     /gene="elbB"
                     /locus_tag="OO609_000326"
     CDS             complement(59233..59886)
                     /gene="elbB"
                     /locus_tag="OO609_000326"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6631870.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60116..62452)
                     /gene="arcB"
                     /locus_tag="OO609_000327"
     CDS             complement(60116..62452)
                     /gene="arcB"
                     /locus_tag="OO609_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6631871.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62548..63477)
                     /locus_tag="OO609_000328"
     CDS             complement(62548..63477)
                     /locus_tag="OO609_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6631872.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64152..68612
                     /gene="gltB"
                     /locus_tag="OO609_000329"
     CDS             64152..68612
                     /gene="gltB"
                     /locus_tag="OO609_000329"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6631873.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68625..70043
                     /gene="gltD"
                     /locus_tag="OO609_000330"
     CDS             68625..70043
                     /gene="gltD"
                     /locus_tag="OO609_000330"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6631874.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
BASE COUNT        17131 a        19022 c        17404 g        16489 t
ORIGIN      
        1 ctggttaccg cgtagcctgg tttcctatca gatgaccagc cagaaagatc gcccaaccca
       61 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
      121 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
      181 ttatggaacc ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg
      241 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
      301 cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg aggatacaaa actatccggg
      361 gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt atcaggaaaa
      421 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgaac aaacaacaca
      481 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
      541 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
      601 cagcaacgat gatacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
      661 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca
      721 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
      781 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
      841 tgatatccac atcctgcgct tatgcctcct caggagggtg tggagccgac agcactagcg
      901 gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca gataacgtga
      961 caggacggga gtttacctct gcaacgctaa gtagcactaa ctggcaatac gcctgttcct
     1021 gctctgcggg taaggcagtt aaacttgtct atatggtcag ccccgtactt accaccactg
     1081 gacatcagac aggatattac aaactcaatg acagcctgga tattaaaacc acattacagg
     1141 caaacgacat tccaggactc acaaccgacc aggttgtctc tgttaacacc cgattcacac
     1201 agataaaaag cagcaccgta tattctgctg caacccaaac gggtgtttgc cagggtgaca
     1261 cgtctcgtta tggacccgtt aatattggtg caaataccac ctttaccctg tatgtcacca
     1321 agccatttct cggctcgatg accattccga aaacggatat tgccgtcatt aaaggcgcgt
     1381 gggtcgatgg aatgggaagc ccgtctacag gtgacttcca tgatttagtc aagttatcga
     1441 ttcagggaaa tctcaccgcc ccacagtcgt gcaaaattaa tcagggcgat gttattaaag
     1501 ttaattttgg attcatcaat ggtcagaagt ttaccacccg caatgccatg ccagacggtt
     1561 ttactccagt agactttgat atcacttatg actgtggtga tacttcaaag attaaaaact
     1621 cgttgcaaat gcgcatcgac ggtacaactg gggtagtaga ccagtacaac ctggtcgcca
     1681 ggcgaagaag ttcagacaat gtgcccgatg tcggtattcg tattgaaaat ctcggcggcg
     1741 gagttgcaaa tattcctttt cagaacggta tccttcccgt tgatccttcc gggcatggca
     1801 ccgtcaatat gcgcgcctgg ccagttaatc tggtcggtgg tgagctggaa acaggaaaat
     1861 ttcagggcac agccaccatt accgtcatgg tgcggtaaaa gaagacgttg cgttttgatt
     1921 aacgcaacgc ttcggcactg ttacccctgc tgctccagcg catacttata cagcgcattt
     1981 ttcttcacgc catgaatttc tgcggccagc gccgccgctt ttttcagcgg cagttctgcc
     2041 tgtagcagcg ccagcgtgcg cagggcatcg gcgggtaagt cttcttcctg tgctttatga
     2101 ccttcgacaa tcagcaccat ttcgcctttg cgacggtttt catcttcctt tacccacgcc
     2161 agcagctcgc caacgggcgc gccgtgaatg gtttcccagg ttttggtcag ctcacgcgcc
     2221 agaaccacgt agcgggattc gcctaatacc gcaacgatat cttccaggct atctaacaga
     2281 cggtgggtag attcataaaa aatcagcgtg cgcggctccg cttcaatggc ttttagcgca
     2341 tcacggcggc cttttgattt ggcaggtaaa aagccttcgt aacagaaacg gtcagatggt
     2401 aaacccgcgg cgcttaacgc agtgatagca gcacacggtc cgggtagcgg caccacacga
     2461 atccccgctt cacggcaggt acgcaccaga tggtagccag gatcgttaat tagcggcgtt
     2521 ccggcatcgg aaaccagcgc aatgttttgc ccctcttgca gctttgccag cagcgtttcg
     2581 gctttttgtt gttcgttatg gtcgtgcagc gcaaacaacc gggcattaat cccaaaatgt
     2641 tgcagcaata aaccggtgtg acgagtatcc tcggcggcaa tcagatcaac ggcctgtaat
     2701 acctctaacg tacgctgggt gatatccgcc agattgccga ttggcgtcgg tacaatgtaa
     2761 agctggccct gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg
     2821 atttaatatt gagcattgcg taaaaaaaat atcactggat acattatggt accctcaaca
     2881 ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc
     2941 ggttgtggca cccatactcc cgatcagtcc actgcttata tgcagggcac ggcgcaggct
     3001 gattctgcct tttatcttca gcagatgcag caaagctctg atgataccag gatcaactgg
     3061 caattactcg ccattcgtgc actggtgaaa gaaggtaaaa ccgggcaggc ggttgagttg
     3121 tttaaccaac taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg
     3181 gcagagatta aactggcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc
     3241 acacctgccg atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa aatcgatgcc
     3301 agccaggggc gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctt
     3361 ggcgcgaaag aaaaacagca gaatattgat gccacctggc aggcgctctc ctccatgact
     3421 caggaacagg cgaatacgct ggtgatcaac gccgacgaaa atattctgca aggctggctg
     3481 gatctgcagc gcgtctggtt tgataaccgt aacgatcccg acatgatgaa agccgggatc
     3541 gccgactggc agaaacgtta tccgaacaat ccgggcgcga aaatgctgcc aacgcagttg
     3601 gttaacgtaa aagcgtttaa accagcctcg accaacaaaa tcgccctgct gttgccgctg
     3661 aatggccagg cagcggtatt tggtcgcact attcagcaag gctttgaagc cgcgaaaaat
     3721 atcggcactc agccagtggc ggctcaggta gctgccgcac ctgccgcaga cgtagcagaa
     3781 caacctcagc cgcaaactgc ggatagcgtt gccagcccgg cacaagcctc ggttagcgat
     3841 ctgaccggtg atcagcctgc ggcccagccg gtgcctgtaa gcgccccggc gacaagcacc
     3901 gcagcggtaa gcgcacccgc aaatccatcc gcagagctga aaatctacga tacctcatca
     3961 caaccactta gccagatctt aagccaggtt cagcaggatg gcgcgagtat tgtggttggt
     4021 ccgttgctga aaaataacgt tgaagagttg ctgaagagca acaccccgct gaacgtactg
     4081 gcactgaacc agccggagaa tatcgaaaac cgcgtcaata tttgttactt cgcgctttca
     4141 ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg
     4201 ctggtgctga tcccacgcag tgcattgggc gatcgcgtag ccaatgcgtt tgcgcaagag
     4261 tggcagaaac tgggcggcgg caccgttctg caacaaaaat ttggttccac cagcgaatta
     4321 cgcgcgggtg ttaacggcgg ttctggtatt gctttaacgg gtagcccgat tactcccaga
     4381 gcgacaaccg actccggcat gacgaccaac aatccaacgc tgcaaaccac gccaaccgat
     4441 gaccagttca ccaataatgg cggtcgtgtc gatgcggtct acattgtggc aacgccgggt
     4501 gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg gtagccagag cggtgcaacg
     4561 ctgtacgcca gttcccgcag cgcacaaggc accgcaggcc cggatttccg actggagatg
     4621 gaaggcttgc agtacagcga aatcccgatg ctggcaggcg gtaatctgcc gttaatgcag
     4681 caggcgctca gcgcggtgaa caacgattat tcgctggctc gcatgtatgc gatgggcgtt
     4741 gatgcctggt cgttggcaaa tcatttctca caaatgcgcc aggttcaggg ttttgaaatc
     4801 aacggtaata ccggaagtct gacggctaac ccggattgcg tgattaacag gaagttatca
     4861 tggctacagt accaacaagg tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg
     4921 atgcgtggga agcacaagcg cgtcgctggc tggaaggcaa aggactgcgg tttatcgccg
     4981 ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc ctgaccaccg
     5041 tttttgtcga ggtacgctat cgccgctctg cgctttatgg cggcgcggca gccagtgtga
     5101 cccgcagcaa acaacacaaa ttattacaga ctgcccgctt gtggctcgcg cgtcataatg
     5161 ggagttttga tactgtggat tgccggttcg atgtggtagc cttcaccggg aatgaggttg
     5221 agtggattaa ggatgccttt aatgaccact cataattaag gtttaaggat tagcgtgcaa
     5281 gaaagaatta aagcttgctt cactgaaagc attcaaactc aaattgcggc ggcagaggcg
     5341 cttccggatg ccatctcccg tgcagccatg acgctggttc agtctctgct caatggcaac
     5401 aaaatcctct gttgtggtaa tggaacttcc gctgccaatg cacagcattt tgctgccagc
     5461 atgatcaacc gtttcgaaac ggagcggccc agcttacctg ccattgcact aaatactgat
     5521 aatgttgtct taacggcgat tgccaacgat cgcttacatg atgaagtgta tgcaaaacag
     5581 gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca tttccacccg tggcaacagc
     5641 cgcgatattg ttaaagcagt tgaagccgcc gttacgcgtg atatgaccat tgtggcattg
     5701 accggctatg acggcggcga acttgcaggt ttgttagggc cacaggatgt ggagatccgc
     5761 attccttcgc atcgtagtgc tcgcattcag gaaatgcata tgctgacggt aaattgcctg
     5821 tgcgatctga tcgataacac gcttttccct caccaggatg attaaggaga atacatgaag
     5881 gcattatcgc caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg tgttgccgct
     5941 gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa ctgacccacg cagtgtcggc
     6001 acccaggtgg acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc gaaagacgaa
     6061 cagattaaga aagaaacccg tattaatgtc acggcctatc agggcaaagt gctgctggtt
     6121 gggcagtcac caaatgctga actttcggct cgcgccaaac agattgctat gggcgtagac
     6181 ggtgccaacg aagtgtataa cgagattcgt cagggccagc cgattggtct gggcgaagca
     6241 tctaacgata cgtggatcac caccaaagtg cgttcgcagc tcttaaccag cgacctggtg
     6301 aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat ggggctggtg
     6361 actgaacgtg aagcgaaagc ggcggcagat attgccagcc gggtgagcgg cgtaaagcgg
     6421 gttaccacag cgtttacgtt tattaaatag cagccccttg taatgcctga tgcgacgctt
     6481 gccgcgtctt atcaggccta caacgatgca aaccgtaggt cggataaggc gtttacgccg
     6541 catccgacat gttttccctc aaatcagaac aacagcgcca gcccgccgac aatcacacca
     6601 gacactgcca ccatcgcccc tgtcagccat aaggctttcg ccgggaacgc tttgcgcagc
     6661 ataatcagtg acggcaaact cactgccggg agcgtcatca acaatgccag cgccggagcg
     6721 gttcccatac ctgccagcat catcgtttgt acaatcggaa tttctgctgc cgtgggaatg
     6781 acaaacaagc atcctgctac cgccatcgcc accacccaca tcaggctgtt atcgacagca
     6841 ccatcggcat gggggaataa ccagacgcgt gcggcaccca acaccagtac tgcaaggatg
     6901 taaaccggga tcgtactcca gaaaagtgtc catagcgccc tgccccagcg gctaaaaaaa
     6961 ccgctctgtg cttccggtat gtcaatttcg accggtgcct gcgtttgcgg tgtttcacgc
     7021 acccattttt gcaccagcgt cgcaatcagc aacaccatca ccagcccggc caccagacga
     7081 atcgccgcaa aaccccagcc gaggacaaac cccataaaca ccagcgtcgc cgggtttaac
     7141 accggattgc ccatccagaa tgccagcgca ccgcccatcg atacctgttg gcgacgcatt
     7201 cccgccgcga ccggagccgc acagcaggta cacatcatgc ccggcagcga aaacagcgtt
     7261 cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac gcaacaacca gtcacgcggg
     7321 atcagcacct gaatcaacga accgaggatc acccccagca ccgccgcttt ccatactgcg
     7381 aggaaataga tcatcgcgta atccaacgcc gcttgccacg gattagcatc cgcctgcgca
     7441 aggatagatt taccgatact gtgggtttcg gctgcagtaa acgctttgcc gtagtaaggt
     7501 tgccatttca cataccagag accggcaatg acaacgagaa agaaaagcgc gggtttccac
     7561 cactgaatgg gcgttgccgc ctgagatgaa gactgaccag tcatagcatt ccccaggaat
     7621 aagttatgat aattgagcgc gtgaatatta cgctcactat caattcttgg gaataattat
     7681 tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca cgccttcatg ctcaggccgc
     7741 atcgcctctg ccagcatgac ccgcgccacg tctctcgcat caatggattt ccagttacct
     7801 ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca tccgctgttt gctacgatcg
     7861 cccagtaaca tcgacgggcg agcaatggtc aatttcggcc agttctgggc aattaatgct
     7921 tcttccatct cccctttgac gcggttatag aaaaacggcg agtgggcatt ggcacccata
     7981 gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc cggttaatgc ggtatccact
     8041 accagcgtgt aatcggcatg aataaacgcc tctttgctcc ccgcttctcg ccgcgtggtg
     8101 ccgagacaac aaaacacaat gtcgatgggt tcggtgacct gcgccaacgc gtcactcagt
     8161 tgcggatcat ggggattaaa caccccgggc atatcgccca acggacgtcg cgtcggcgca
     8221 gcaatggcgt taactttcgg ttcattaatc aacatccgca gcaggtgacc gcccaccagc
     8281 cccgttgcgc ctgtaatcag tacctgactc atcttcgttc ctttacagaa ttgtctgcct
     8341 tgcgctcccc ggctcatgca ccagtcttaa tagaccatga ggtaattatc cccgattgtg
     8401 gaaaattcgc ctcatccaat gcaacaacgc ggaggaagca tgagtaagaa aattgccgtt
     8461 ttaatcactg atgaatttga agattcagaa tttacttcac ccgcagacga gttccgtaaa
     8521 gccggacacg aagtgattac tattgaaaaa caagcgggta aaacggtgaa aggcaaaaaa
     8581 ggagaagcca gcgtgaccat cgataaatcc atcgatgaag tgacgccagc ggagtttgat
     8641 gccctgttgc taccgggtgg tcattcaccg gattatctgc gtggcgacaa ccgttttgtc
     8701 acctttaccc gtgattttgt gaatagcggc aaacctgttt tcgccatctg tcacggtccg
     8761 cagctgctga tcagcgccga tgtgattcgg gggcgcaaac tgaccgcagt taaaccgatc
     8821 attattgatg ttaaaaatgc gggcgtggaa ttttacgatc aggaagtcgt ggttgataaa
     8881 gatcagctgg ttaccagccg gacaccggac gatctgccag cgtttaaccg cgaggcgtta
     8941 cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt ttaccaaagc ccagcgtgtt
     9001 gtcggtgaat ttgatttcat cgagccggat ttcccacacc ggggccgaca gcattctggc
     9061 aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg ctttcctcac cttccagcct
     9121 gcggatctca cctttaaact gcacaccgcg aattaacgct accgttttcg gctgaccgtt
     9181 taccgttccg gcaaccgcag cctgcggccc actcatctgc gcgtggcgcg ttttttcttc
     9241 cgtcaaaatg tagaaggcga ctttctgcgc atcaaaaaga taaaaggcat tagcgcacca
     9301 gagttcccct tcctgctgca cacaccaggt gacaacatgt tgttttgcca gccaacggct
     9361 gatggcgatg agtgtttcca ttgctgttct cttttatact gtgggcctga ccttaacatg
     9421 cgatgcagac gatgacaccc tggtttcttt acttgatccg taccgccgac aataagcttt
     9481 ataccgggat caccacggat gtcgaacgcc gctatcagca gcaccaaagc ggcaaagggg
     9541 cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt ttctgcgcca gtgggcgacc
     9601 gttcgctggc actacgggcg gaatatcgcg ttaagcaact gaccaaacgg caaaaagagc
     9661 gtctggtagc ggaaggcgca gggtttgcag aactgttgag cagcctgcaa accccggaga
     9721 ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg gttacgccat tcagcgcgtc
     9781 atccgctaaa cgatgtacca ggaaggcgct ttcagtgccc ggccagcggc aacgcagatc
     9841 gtgatgcgcc gccagttcaa agccgaaacg actgtacagc gccggatcgc ccagcgtcac
     9901 cactgcggca tagccgaact cattaagcga atcgagtcct tcatagacca gttggcgtgc
     9961 cagcccctgt ccacggtatt tttcatcaac cgccaacggt gccatgccga cccattgcag
    10021 gtcttcgccc tgcacatcaa ccggactaaa tgccacatag ccaatgacct gaccttcgtc
    10081 atctgtcgcc accagcccca gcgtcagaaa gccatcttca cgcagatcgt gaaccagctt
    10141 cgcttccgca tcactttcga atgagcgacg cagcagggca tcaatacccg gcgcatcaat
    10201 gggaatttct actcgaatta gcatggttca cctaccgatg tctgtttggt ttcaggcgcg
    10261 gttttcattc ccgcctcaac aaaatccgcc agttgcagca gcatcatgcg taaggctttc
    10321 ggcatttgct ccagttcaat ggcgtccatc aggtttttca catacacccc cagctccgta
    10381 tcgccttcaa tcaccagccg acgctggaag aagagcgtat ccggatcttg tttacgcgcc
    10441 gcaatcatca gcagatcgct ggcgtcggca ctaaaactca catcagcttg cgtgttctgg
    10501 ctgaccacca gtttgccatt caccaccgag gtaaaccatt gcaggtcaat atcacgcaca
    10561 tgaatactta accagcggcc ttcaagaaac tccagctcgc catcatccag cgcctgacgg
    10621 aattgccagc ttaagacctg ctcaagaacc tggcgtttta gcgcaaatgg cgtcagtttt
    10681 accggtacac tcaacagaga tggccccaaa tgcacaatac gggaacgcag tttatccaac
    10741 acgagtttta ctccctgttt caacaatcat cctattttgc catatcagaa aaataacata
    10801 gcggtataaa tcaacaattc catatgaaat tgctgctacc accaatacaa ctttaactgc
    10861 cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc cagttcgtta acatttttgc
    10921 gttttgatag cgcaaccttc aggaaaaatt atggagctgc tctgccctgc cggaaatctc
    10981 ccggcgctta aggcggccat cgaaaacggc gcagatgctg tttatatcgg gctaaaagat
    11041 gataccaatg cccgtcactt cgccggcctt aactttaccg agaaaaaatt gcaggaagcg
    11101 gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg cgattaacac ttttgcgcat
    11161 ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg cggcgcagct gggtgccgac
    11221 gcgctgatcc tcgccgacct cgccatgctg gagtacgccg ccgagcgtta cccgcatatt
    11281 gaacgtcatg tgtcagtgca ggcttcggcg accaatgaag aggcgattaa cttttatcat
    11341 cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt tgtcgattca tcaggtgaaa
    11401 cagctggcac gggttactcc tgtcccgctg gaagtgtttg ctttcggcag cctgtgtatt
    11461 atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg gtgagtcgcc caacactgtg
    11521 ggtgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa cgccgcaggg gctggaatcc
    11581 cgcctgaacg aagtgctgat cgaccgttat caggacggcg aaaacgcagg ttatccgacg
    11641 ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc acgcgctgga agaaccaacc
    11701 agtctcaata ccctggaact gctgccggag ttaatggctg cgaatattgc ttcggtgaaa
    11761 attgaaggcc gccagcgtag cccggcgtat gtcagccagg cggcgaaagt ctggcgtcag
    11821 gctatcgacc gttgtaaggc cgatccgcaa aacttcgtac cgcaaagcgc gtggatggag
    11881 acgctcgggt cgatgtccga aggcacgcaa accacccttg gcgcgtatca ccgtaaatgg
    11941 cagtgagaag agcaatgaaa tattccttag ggccagtgct gtggtactgg ccaaaagaga
    12001 cgctggaaga tttttatcag caagccgccg ccagcagcgc cgacgtgatt tatcttggtg
    12061 aagcggtatg cagcaagcgc cgggcaacca aagttggcga ctgtctggag atggcaaaat
    12121 cgctcgccgg aagtggcaag cagattgtgc tctccacgct ggcgctggtg caggcatcct
    12181 ctgaactggg cgaactgaaa cgctatgttg agaacggtga atttctgatt gaagccagcg
    12241 atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc gttcgtcgcc gggcacgcgc
    12301 tgaactgcta caacgcggtg acgctgaaaa tattgctcaa acagggcatg atgcgctggt
    12361 gtatgccggt ggagctttcc cgcgactggc tggtgaatct gcttaatcag tgcgatgagc
    12421 tgggcattcg caaccagttt gaagtggaag tcctgagcta cggtcatcta ccgctggcct
    12481 actccgcccg ctgctttacc gcgcgttcgg aagaccgccc gaaagatgag tgtgaaacct
    12541 gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca ggaaaaccaa caagtgtttg
    12601 tactcaatgg cattcagacc atgagcggct acgtttacaa cctcggtaac gagctggcat
    12661 ccatgcaggg attggttgat gtagtccgcc tgtcaccgca gggcactgac actttcgcga
    12721 tgctcgacgc cttccgcgct aatgaaaatg gcgctgtgcc actgccgttg acggcaaaca
    12781 gcgactgtaa cggctactgg cggcggctgg caggactgga gctgcaagcc taagtaaata
    12841 gctcactttg ttaacaactt taactattct ttaatgcagt attaaagatt aatcggtaac
    12901 aaagtgagct gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat
    12961 acccgaaggc tcttcagcgc gggaagcgtt ctcccactct ctcgatctcg cccgtctggc
    13021 tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga ccggcattgc
    13081 cagtgctgca acatcggtat tgattggcta tctggcggcg aataccacca cgctgcatct
    13141 ggggtctggc ggcgtgatgt tgcctaacca cttaccgttg gtcattgccg aacagttcgg
    13201 cacactcaat acgctctatc cggggcgaat cgatttgggg ctgggtcgtg ctccgggtag
    13261 tgaccaacgg acaatgatgg cgctacgtcg tcatatgagc ggcgatattg ataatttccc
    13321 ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg
    13381 cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag
    13441 tgcacaactg gcggcgcagc ttggtctgcc atttgcgttt gcctcacact tcgcgccgga
    13501 tatgttgttc caggcgctgc atctttatcg cagtaacttc aaaccgtcgg ctcggctgga
    13561 aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga
    13621 atttctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccgggca
    13681 actgccgccg ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatgacgt
    13741 gcagcaggcg ctgagtatgt cgttggtagg cgataaagcg aaagtgcgtc atggtttgca
    13801 gtcgatcctg cgcgaaaccg acgctgatga gattatggtt aacgggcaga ttttcgacca
    13861 ccaggcgcgc ctgcattcgt ttgagctggc gatggatgtt aaggaagagt tgttgggata
    13921 gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg taggcctgat
    13981 aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt
    14041 actgatacac cggcagtaaa ttaaagctcg ataaaatatg caccagtgcg ttgccgacgc
    14101 caaacaccag aatcagcgca atcatcggct tgccacccca gacgcggaat ttcgggctgc
    14161 caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc cagatggtag
    14221 ccgctaaacc agcataacca atggcataca ggaatccgtt cgggaacagc agaccaccca
    14281 caacaggcgg ggcaaaggtc agcaatgccg ttttcaagcg gcccacagcc gagtcgtcga
    14341 aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg
    14401 ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc
    14461 cgcttaatgc ttgtaccaac acatcaatat taccgccctt ctcggcgatg ccgataaact
    14521 ccggacgagg gatgttgccc attgtcgcca gcaaccagat ggtatacagc gccagtgcca
    14581 tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca
    14641 tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc agggtcatca
    14701 gcaaatacgg cgcataagat gcattgcttt cggcaacgtt gaacaatgtc gcaggctgca
    14761 catgccccag caggctaccg aaggtgagga agaaggtaat gactttcgcc cccagcacaa
    14821 tcgccgtcat gcggctgacg gctttagtac tcaaccacac cacaaacgct accagcagtg
    14881 caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat
    14941 gcagaatcga accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa
    15001 tggaaatgcc gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa
    15061 aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca
    15121 tacagaacca ggtaaagatc agcgctgcca ttgaccagaa aaaccacgcc ccggacatga
    15181 ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc
    15241 caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct tctctccagt
    15301 gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacatga gtacaaaaga
    15361 cagaaaaaaa gccccgatgg ttaaaatcgg ggctgtatat attattttac agattgtgtt
    15421 cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac
    15481 gacgcggtgc gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacgaccac
    15541 cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgctcgcca ccgaaaccac
    15601 gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt
    15661 tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac
    15721 ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttaccg atgtaacggc
    15781 tgctgatgtc gccttcgtta gcaatcgcgc caacgatatg acgaacttca acaccatcat
    15841 cgcggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg
    15901 gacgatcttc acggtcgcca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat
    15961 cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggaaca atcagagtac
    16021 gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac
    16081 cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt
    16141 ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg
    16201 cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca
    16261 gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg
    16321 tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag ttaaccacca
    16381 ggctgatacg ctcaacgtcc aggccacggg ccgcaacgtc ggtcgcaatc aggatgtcca
    16441 gacgaccgtc tttaaggcgt tccagtgtct gctcacgcag cgcctggttc atgtcaccgt
    16501 tcagcgcggc gctattgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt
    16561 ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca
    16621 gtgcttcgtt tttacgcata ccccacacag tccagtagct ctggctgatg tcaggacggg
    16681 tagtcacgct ggactgaata cgcacttcct gcggctcttt cataaagcgg cgggtaatgc
    16741 gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct
    16801 gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat
    16861 ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca
    16921 gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt
    16981 cataacgctg gccgccgtac agggcaacca catttacgcc gcgcatgtgt ttagagaaat
    17041 ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct
    17101 gtggtgcttt cagctcagga tcaagattct gcagcagagg taaagagaat gctgcagttt
    17161 ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac
    17221 actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag
    17281 gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc
    17341 tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt
    17401 gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta
    17461 aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga
    17521 ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg
    17581 ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc
    17641 caaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc
    17701 agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct
    17761 aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc
    17821 gaagtgctgt ttcaatactt ctttagcctg cttctcatcg agcttctgct cggcgagata
    17881 aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc
    17941 ttgcgctaac ttgtcacgac cgccgtaata taatgcaatc ccgcgattca agtgcgcgta
    18001 gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt
    18061 gcctgcctgc gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat
    18121 tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa
    18181 cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag
    18241 gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc
    18301 gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc
    18361 aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga
    18421 acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc
    18481 ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc
    18541 aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac
    18601 ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac
    18661 gcgtttgtca gcgatttgag agatgtgtac cagaccttct ttaccgccgc cgatggcaac
    18721 aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat
    18781 ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc
    18841 tgcaatcttc acagtaccgt catcttcgat ttcgatggtg gtgccagttt cttcggtcag
    18901 ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg ggttgatctt
    18961 gatggtatgg atacgtggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg
    19021 ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat
    19081 gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga taccctcgcg
    19141 ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga
    19201 cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc
    19261 tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga
    19321 agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg ggaatttgtc
    19381 catatccggc atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg
    19441 acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg
    19501 gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc
    19561 agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca gcgcagaacc
    19621 gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg
    19681 accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatcgc
    19741 gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc
    19801 gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg
    19861 gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt
    19921 taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca gttcattgat
    19981 gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca gcatctggtc
    20041 ttcgctcagc agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac
    20101 aaccagatcc agtttgctct ctttcaactc gtcctgagtc gggttcagta cgtactggtc
    20161 attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac cagacagaga
    20221 cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg ggttaacaga
    20281 aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcggccgaat
    20341 cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttgggcggc cttcacgacg
    20401 gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag
    20461 tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa caaataccgc
    20521 ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc
    20581 cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat
    20641 atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg
    20701 catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc
    20761 cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat
    20821 tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa
    20881 ttagcgacgc agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt
    20941 caggtagtcg agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg
    21001 gtgatctttt ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag
    21061 tgctacctga acttcagtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat
    21121 tttagctgtt gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag
    21181 gacgccgatc tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac
    21241 gcgacgttaa gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt
    21301 acgccgggta ttcaaccacc aggcgacgag gcgcaacgcg gccttcatcg tcaatttcgc
    21361 ccataccgat aaatttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg
    21421 caccggatgt acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat
    21481 tcaccaccgg gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact
    21541 ccgcagccgg aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg
    21601 tcacaatccg ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg
    21661 cgccacaacc gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg
    21721 agcagtgaat ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa
    21781 cggtaatcgg gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt
    21841 ttttaccctg atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga
    21901 aagtatccag cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga
    21961 tttgtccgtc ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc
    22021 gtttgtcgga gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca
    22081 acatgccagt tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa
    22141 tccgtttcac tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca
    22201 aaacgccgtt aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc
    22261 gtccgggtta acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat
    22321 gcgcatccct tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag
    22381 gcgcatcgct ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat
    22441 gcccgcttta accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata
    22501 cgccaggtcg cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc
    22561 tttaatttca cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg
    22621 cgggcgacca aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc
    22681 ctttttaaaa ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag
    22741 cgtcacatca ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct
    22801 aaaaacctta agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc
    22861 cagtgcggac gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt
    22921 cgttaacgtc atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt
    22981 tgtcacgcag aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc
    23041 ctgcgatggc accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa
    23101 tctgctgttt cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca
    23161 ggttatagat gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg
    23221 cagaggcatc agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg
    23281 tggcgtcggt ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt
    23341 cagtagacag tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct
    23401 tcaggacgat gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga
    23461 gtttagattt ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg
    23521 caacttcacg cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta
    23581 cgccggacag gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac
    23641 ccagttcgtt acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc
    23701 ccttgtgcag agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga
    23761 ggaaggattc gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa
    23821 cttccgcctg cagcaggata gcgtccagca gttcatcgat accagtaccc gctttcgcag
    23881 acacatgtac gaactggctt tcaccgcccc actcttccgg caggatgccg tactgggaga
    23941 gttcgttctt aacgcgatcc ggatcggctt ctggtttatc gatcttgttc actgcaacga
    24001 ccaccggaac ctgcgccgct ttcgcgtgct ggattgcttc gatcgtctgc ggcattacgc
    24061 cgtcgtcggc agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgagcacgca
    24121 ttgaagtaaa cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt
    24181 caacgtggta tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gccactttcg
    24241 ttgaacgaat gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca
    24301 caaccggcgc gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt
    24361 ccagctcgtt ttcacgacgc aggataactt tatggcccat ctcttcagca accagctgtg
    24421 cggtttcctg atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt
    24481 tgatgacctg agagccttta accgccatct tgttcgccag ttcgccaacg gtgatagttt
    24541 cgccgatcac aacgtcacgg ttaacggcct gagcaggctt ctggaagcct tgctgcagcg
    24601 aagaaccttt acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat
    24661 cagcttttga ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac
    24721 cacggccacg gccgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt
    24781 gagaagtagt gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt
    24841 tgttttcttc cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt
    24901 tacgacgcgc ttcttcttca gctttacgct tgagctctgc agcttcctgc tcacggcggg
    24961 ctttttcagc ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt
    25021 ccgcagcttc acgtttcgct tgttcagcag cttcacgctt agcttgttct gcggcctcac
    25081 gttcagcttt ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct
    25141 cttccgctgc aaggcgttca gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc
    25201 ggacttcgat ttgtaccgat ttgctttttc caccggtacc aggaatgtta agggtgctgc
    25261 gtgttttacg ttgcagcgtc aatttgtccg ggcctgaatt tttctgattc aggtggtcaa
    25321 tcaaagtctg tttctcttgt gcagacacag agtcgtcagc agacttccgg atacctgcat
    25381 cagcaaattg ctgtaccagg cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa
    25441 tcgttacatc tgtcatgctg ttccttcctg ctacagttta ttacgcttcg tcaccgaacc
    25501 agcaaatatt acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc aacccttcga
    25561 tatcagccag atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa acgccacggg
    25621 cggccagttt gaatgccaaa tcacgatcta ccccttcaag gttcagcaga tcgtcagccg
    25681 gtttgttatc accgaggctt tcttcctggg cctgtgcaat ggtggccagt gcatttttag
    25741 cacgctcgcg cagtgcttca acggtcggct catcaaggcc ttcgatttcc aacagctctt
    25801 tcatcggcac ataggccagt tcttccagcg tcgagaaacc ttcttctacc agaacagtcg
    25861 cgaagtcttc gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg
    25921 cctgatgctt agcctgcagg tcgtcaacgg tcattacgtt gagttcccaa ccgctcagct
    25981 gcgaagccag acgcacgttc tgaccgttac ggccaatcgc ctgcgccagg ttaccagctt
    26041 caacggcgat atccatggtg tgtttatctt catccaccac gatagaagca acgtctgccg
    26101 gtgccattgc gttaatcaca aactgcgccg ggttatcatc ccacaggacg atatcgatac
    26161 gctcgccgcc cagttcagta gaaaccgcct gaacacgcgc gccacgcata cctacgcaag
    26221 cacctaccgg atcgatacgt ttatcgttgg ttttcaccgc gattttcgca cgagaacccg
    26281 ggtcgcgagc cgctgcttta atttcaatca cttcttcgcc gatttccggc acttcaatac
    26341 ggaacagttc gatcagcatt tccggcttgg aacgagtgac gaacagttgc gcgccacgcg
    26401 cttccgggcg aacggaataa agcacgccac gaacgcggtc gccagggcgg aagttttcac
    26461 gcggcagcat atcttcgcgc aggatcacgg cttcagcgtt gttgcccaga tccagagaga
    26521 tgttgtcgcg gtttactttt ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga
    26581 actgatcaac caccatcgca cgttcggctt cacgcacttt ctgcacgata acctgttttg
    26641 ccgtctgggt ggtgatacgg tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc
    26701 ccaagttcag gctttcatct tcataacgtg cggcttcaag ggtgatttcc ttggtcggct
    26761 gggtgacttc atcaacaact aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat
    26821 cgatctgtac acggacgtcg atctcttgct catatttttt ctttgttgct gtcgccagcg
    26881 cgctttccaa tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg gatacggctt
    26941 caactacagc caaaatttct ttgttcatcg cgggcttttc acctcatcca gactattaaa
    27001 agtggggaac caggttcgcc ttctggatat tactcagcgc gaacacttca tctttacctt
    27061 cgacggtaac tgtgatcatt tcaccgtcta ccgctttgat aacgccctgc catttacgac
    27121 ggttttgtac cgccatacgg agaaccagag tcacctcttc tccgacaaaa cgggcgtagt
    27181 gttcagccgt gaacagtggg cgatcgagac ccggtgagga gacttccagg ttataagcaa
    27241 cggtgatggg atcttcaaca tccagcacag cacttacctg gtggctcaca tcagcacaat
    27301 catcaacatt gatgccatct tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc
    27361 gaataaattc gatgcctacc agttcaaaac ccagggcctc aactggcgca gtaatcatct
    27421 ctgttaattt ttgctctaat gtggacaagc ccacccccaa gacataaaaa aagggcctaa
    27481 agcccagtta ttctgtagtc agataacaaa aaaccccgat aaatcggggc tttatataac
    27541 tgaaccctat aaccgcaact gcggtctgga gcactttcca gaaggatttt ttcaaatccc
    27601 actacgaagg ccgaagtctt cacagtatat ttgaaaaagg actctaaggg aaagtggttg
    27661 cgggggccgg atttgaaccg acgaccttcg ggttatgagc ccgacgagct accaggctgc
    27721 tccaccccgc gcctgaaacg tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact
    27781 gctgggattt ggtgtaccga gacgggacgt aaaatctgca ggcattatag tgatccatgc
    27841 cacattttgt caacgtttat tgctaatcac gtgaatgaat atccagttca ctttcatttg
    27901 ttgaatactt ttgccttccc ctgctctccc ttaagcgcat tattttacaa aaaacacact
    27961 aaactcttcc tgtctccgat aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa
    28021 attcagtgag agcggaaatc caggctcatc atcagttaat taagcagggt gttattttat
    28081 gacgacgatt ctcaagcatc tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg
    28141 tctggacacc agtgccgcac tgctgtggat gcgacaaaag ggagcggttc cttatgcata
    28201 tactgcaaac ctgggccagc cagacgaaga ggattatgat gcgatccctc gtcgtgccat
    28261 ggaatacggc gcggagaacg cacgtctgat cgactgccgc aaacaactgg tggccgaagg
    28321 tattgccgct attcagtgtg gcgcatttca taacaccacc ggcggcctga cctatttcaa
    28381 cacgacgccg ctgggccgcg ccgtgactgg taccatgctg gttgctgcga tgaaagaaga
    28441 tggcgtgaat atctggggtg acggtagcac ctataaagga aacgatatcg aacgtttcta
    28501 tcgttatggt ctgctgacca atgctgaact gcagatttac aaaccgtggc ttgatactga
    28561 ctttattgat gaactgggcg gccgtcatga gatgtctgaa tttatgattg cctgcggttt
    28621 cgactacaaa atgtctgtcg aaaaagccta ctccacagac tccaacatgc ttggtgcaac
    28681 gcatgaagcg aaggatctgg aatacctcaa ctccagcgtc aaaatcgtca acccgattat
    28741 gggcgtgaaa ttctgggatg agagcgtgaa gatcccggca gaagaagtca cagtacgctt
    28801 tgagcaaggt catccggtgg cgctgaacgg taaaaccttt agcgacgacg tagaaatgat
    28861 gctggaagct aaccgcatcg gcggtcgtca cggcctgggc atgagcgacc agattgagaa
    28921 ccgtatcatc gaagcgaaaa gccgtggtat ttacgaagct ccggggatgg cactgctgca
    28981 cattgcgtat gaacgtctgt tgaccggtat tcacaacgaa gacaccattg agcagtatca
    29041 cgcgcatggt cgtcagttgg gccgtctgct gtaccagggg cgttggtttg actcccaggc
    29101 gctgatgctg cgtgactctc tgcaacgctg ggttgccagc cagatcactg gtgaagttac
    29161 cctggagctg cgccgtggga acgattattc aatcctgaat accgtctcag agaacctgac
    29221 ctacaagcca gagcgtctga cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg
    29281 tattggtcaa ttgaccatgc gtaatctgga tatcactgat acccgagaga aactttttgg
    29341 ttatgccaaa actggcctgc tttcctcctc tgccacttca ggcctgccgc aggtggagaa
    29401 tatggaaaac aaaggccagt aattcgacat caaccctgcc cttcaggggt ggggttacct
    29461 tgcttatcct ctgtaaactc ctttctgagc atcacataat cgaaaacatc atggtttgca
    29521 tccacaaccc gtgagtctcc tttatagtgt gcaatcactt cctcaagcgt ttgcggctgt
    29581 tccggctttg ttacccccat ccacgcatta ataaggtaat tattgtaagc tgtcgaaaag
    29641 atattgtttt ccgttgtgcg atccacgcca tccccaagaa cggggctata ccagataaac
    29701 atcgggacat gatatgcctg ctggctagcc tccctgcctc catgaaaata gacgttcttc
    29761 ttcgtagggt cacgttccag gccgtggtcg gcaaaataca tgaccgaggc gcggcgatct
    29821 tttaataatt caaaaacctg acctagcaaa ctatctgtgt aatgaatgga gttgtcatag
    29881 caggcatcct gatcgtcctg cggttgaaac acggcgctgg attgcggata ggcgctacaa
    29941 gccggttcat ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc
    30001 tgtaatgctt gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt
    30061 tccatggcgc gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc
    30121 gagcttagcc agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg
    30181 taattatgaa tgtcatgact caaaacagaa tcagcagtta aagaaagggg aaccgacagc
    30241 gcagtgtaag gtgcgccgct tattgcttga ttaaacagtt tgatctgttt tctttgtgct
    30301 tcaacttgcg gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca
    30361 gactccccca caatcaacac gtaggtatca atacttgtat ccctgactga taattgaaaa
    30421 tacggcaccg tgtttgcaat tgagagtaat ctttgatgcg ctttcgctgc taaagcaaaa
    30481 tagttgagat tgaaaaacgg cgtataggta gcaaatcacg acgctagtat atatggactg
    30541 aacgcttttt tattttttgc atctttataa gcaaattgac aagcggaaaa taaactgccc
    30601 gaaatgacaa tcagcaataa tattcctgtc actttttttg tcggcaagga aacatcatat
    30661 tttattatta ctgccaaaaa aagaagggat aaaaaagcaa aagcacatag ataagggata
    30721 tacatcccca gcattttcac aacttcatcc ggatcactct gcagcacact aatcgcaaat
    30781 ccatcattga aagttgtacc aaaggtacac catgaataga ggctaatact catgtctgcc
    30841 gcagtcagta acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt
    30901 ttaacaacca ggatggtcag gacgaagaac agcaatcgct gaaccatatg cgctccggaa
    30961 gaggcgacta agttcgtaat accaaaaaca ataacacaaa gatacaacaa ccaatgagat
    31021 ttaaaatctt ctagcaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
    31081 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgatcca
    31141 tatcagaccg aaacactgtt agttttttct ttttaaatga tgcgaccatt gcgttctcag
    31201 taattaaata acattatcaa tgcgtctcta aacattacgc agaacgtagc catcagtagt
    31261 tatactgaca ggagcaggat ggtttatcga gaagtaaaca tatccgtatt cgtaccggaa
    31321 ctggagttga cacaataaag tgccaattat gtcagcagta gggaaaagtt tataactaag
    31381 gcgtaccggc accatcgttt caaggtacca gctacgagta aagcaactgg acgagataca
    31441 gatacctgac aaccattcat cagaccagga ccaaaacgaa aagagacgct tttcagcgtc
    31501 tcttttctgg aatttggtac cgaggacggg acttgaaccc gtaagcccta ttgggcacta
    31561 ccacctcaag gtagcgtgtc taccaattcc accacctcgg cacggatact actttttagt
    31621 tcgggatatc gctggtcggc ttagccggag cagctggctg agtttgttcg gttttcgccg
    31681 gtgcactcag attttcccat tcgctacctc tattggtttt gttgctattg atgttaccca
    31741 gcaccagact gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg gtcatgaagt
    31801 taccagaacc acttgaacca aacagcgtag cggaagcgcc tgctccgaag gaggctccca
    31861 tatcagcgcc tttaccttgc tgcagcatga tcagaccaac aaggccaatt gccacaataa
    31921 ggaaaactac taaaagagct tcgtacataa tcaacctgtt ccttgcggag ttgccgcgta
    31981 ccaatgcttc aaccaataaa gcgggagttt ttaatctttc ccactgaagc gggtgtgaat
    32041 actaaccaaa acgaatgacc ttcgcaaggg caattttatc gcattgtatc aactgcggaa
    32101 aaaaacagca aaaccgatgt gttcgccgag aaaaaaggcg acaatcgccg cctttttagc
    32161 cagttatcta acgctttaaa cggcttttac tgcatcggcg atgcggtgtg caaattcagt
    32221 cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg tgccggattt
    32281 acgcagcaac acgcgtccac ggttgcccag cgcagcttca acctctgcgg tcacggcttt
    32341 aactgactca tgctcaagcg gatcgccgct acctgcggtg taacgtacgt taaccagaat
    32401 ctgcgggaac attttcatgc cgctgcaaag gtcgtgcagg ctcatatggt tacgtgccat
    32461 cgccgccagc acctgcaagc cagcaacgat gccgtcaccg gtagtggttt tatccagcag
    32521 gatcacatga ccggaattct ctgcaccgat acgccagcct ttctcctgca ttttttccag
    32581 tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt tcagcgccag
    32641 ttcaagcccc atgttgctca tcaatgtacc cacagcacca ccacgcagct ggccctgacg
    32701 aagaccttca cgcgcgatga tatacatgat ctgatcgcca tcgactttat tgccttcatg
    32761 gtcaaccata atcacgcgat cgccgtcgcc gtcgaaggca ataccgagat ccgctttttc
    32821 agccagcaca cgagcctgga gcgcgcgaac gtcggtagcc cccacttcgg cattgatgtt
    32881 tacaccgttt ggctcacaac cgatagcgat aacgttcgcc cccagttcgc gcagcacgtt
    32941 cggcgcgatg tgataagtcg caccgtttgc acaatccacc acaatcttca gttcactgag
    33001 gctaagttcg ttcgggaacg tggctttgca aaactcgata tagcgacccg cggcatcaac
    33061 gatacggctg gctttaccca gttcagccga atcaacgcag ctgatctcct tttccatttc
    33121 cgcttcgatg gcctcttcta ccgcatccgg cagtttagtg ccgtcgatag agaagaattt
    33181 aatcccatta tcgtagaacg ggttatgcga tgcagatatc acaattccgg cctctgcgcg
    33241 gaaggtacgc gtcagataag ccacggccgg tgttggcatc gggccagtga agagtgcgga
    33301 aaggcccgct gccgccagac ccgcttccag tgctgactcc agcatatagc cagaaatacg
    33361 cgtgtcttta ccaataataa tcttacggga gccgtggcgc gccagcactt tacccgcggc
    33421 ccaacccagc ttaagcacaa aatcaggtgt gatcggcgca tcccctacac gaccacgaat
    33481 cccatcggta ccgaaatatt tacgattact catagcgttt gttttccttt gcagacagag
    33541 tggcttccac cacccgcatc gcttctacgg tttctttgac gtcatgaaca cgaatgatgt
    33601 gcgcgccttg cattgcggca atgaccgcac aggccagact accgctcagg cgctcggacg
    33661 gccccacgtt cagcagctgc ccaatcatcg attttcgtga catacccacc aacagcggca
    33721 ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga atagttatgg gagagatttt
    33781 taccgaaacc gaatccgggg tcgagcaaca atttctcttt tgcgataccc gcctgctcgc
    33841 aacgtgctat ttgctcaata aagtagcgat tcacttctgc aaagacatcg tcatacttcg
    33901 gagcttcctg catggttttt ggatttccct gcatatgcat cagacaaacc ggtaaaccgg
    33961 tttctgcagc cgcctccaga gcgccaggtt cggaaaggga gcggatatca ttaataatgt
    34021 gagcgccaac tttcgctgac tcacggatga cttctggttt ggatgtatcg accgagatcc
    34081 agacttcgaa gcgttgagca attgcctcaa ccacaggaat aacacgttgc aactcttctt
    34141 caacgctaac ttccgccgcc cctgggcgcg tggactcgcc accaacgtca atgatcgtcg
    34201 cgccagcatt gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag ttatgcgtgc
    34261 caccatccga aaaggaatca ggcgtgacgt tgaggatccc cattacgtga ggatggctaa
    34321 ggtccagtga agtaccctgg gcaaagagtt tcattatgtt atccctggta tgaattgata
    34381 agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa atacagtcat ctgatgcggg
    34441 aacttacttg tcgcctaact gctctgacat ggtgttaccc gggttcggcg tacgcggttc
    34501 atcaaccgga cgaggagcct ttggactacc attgtcgcca gaattgttag aagcgcctgg
    34561 ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat caatctgcgg
    34621 tgcgtcgata gtctcatatt tcatgagagc atctttcatc gcatgcagaa tatccatatt
    34681 gtcggtcaga agctgacgcg cacgattata gttacgctca atcagtgctt tcacttcctg
    34741 gtcgatgata cgtgcagttt catcggacat atgtttcgct ttcgctacgc tacggccgag
    34801 gaatacttca ccttcttctt ccgcgtacag cagtggaccc aatttctcag agaagcccca
    34861 ctgagtcacc atgttacgtg ccaggttggt cgcaacttta atatcgttgg acgcaccggt
    34921 agaaacatgt tccggcccgt agatgatctc ttctgccaga cgaccaccgt acagcgtaga
    34981 aatctggctt tccagtttct gacggctggc gctgattgcg tcgccctcag gcaagaagaa
    35041 agtcacaccc agcgcacgac cgcgtgggat aatcgtcact ttatgcaccg gatcgtgttc
    35101 cggcaccagg cgaccgataa tcgcatgacc cgcttcgtgg taagccgttg attctttctg
    35161 cgcttccgtc atcaccatgg agcgacgttc cgcacccatc atgattttgt ctttcgcttt
    35221 ctcgaactca accatcgaca caacgcgttt gttgccacga gcagcgaaca gtgccgcttc
    35281 gttcaccagg ttcgccaggt cagcaccgga gaaaccagga gtaccacgag caatgattgc
    35341 cgcgtcgata tcgggtgcca atggtacgcg acgcatgtga actttcagga tctgctcacg
    35401 accgcgaaca tctggcaagc cgaccacaac ctggcggtcg aaacggccag gacgcagcag
    35461 ggccgggtcg agaacgtccg gacggttagt cgcggcgata acgatgatac cttcgttacc
    35521 ttcgaagcca tccatctcaa ccagcatctg gttcagagtc tgttcacgtt catcgtgacc
    35581 accgcccaga ccagcgccac gctggcggcc tacggcgtcg atttcatcga taaagatgat
    35641 gcacggtgcc gctttcttcg cctgttcgaa catgtcacga acacgggatg cacccacacc
    35701 gacgaacatt tctacgaagt cagaaccgga gatagtaaag aacggaactt tcgcttcgcc
    35761 tgcaatcgct ttcgccagca acgttttacc ggtacccgga ggaccgacca tcaggacgcc
    35821 tttcgggatc ttaccgccga gtttctggaa gcggctcggc tcgcgcagat actctaccag
    35881 ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg tcgttttgat
    35941 ctgatcttcc gtcagcatgc gcgctttgct cttaccaaac gacatggcac ctttgccacc
    36001 gccgccctgc atttgacgca tgaagaagat ccagacacca atcagcaaca gcatcgggaa
    36061 ccaggagatg aagatagaag ccagcaggct tggttcttca ggcggttcac cgacaacctt
    36121 gacgttcttg gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa tgtaagtggt
    36181 ataacggtta ctatctttct tggtaacgtt gatttcacgt ccgttgatac gcgcttcacg
    36241 aacctggtcg ttattgacct cttgtaggaa ggtagagtaa tccaccttac ggccattaga
    36301 ctcgctgggc ccaaagctct ggaatactga catcagcaca acggcaatga ccagccagag
    36361 tattaggttt ttcgccatgt cactcaaggg attaacctct tattacaact gtgttaaaaa
    36421 cagcgtcagg atactctata tccagcatct ttcaaacttt cgtctgaaat ctcccggtta
    36481 gggtttacgc ccggtcgcta caatatacac ttctcgcgaa cgtgcacgag aagagtccgg
    36541 cttacgaact ttgaccttcg taaacaggga gcgaatttcc cttagatact catcgaaacc
    36601 ttcgccctgg aacaccttca ctacaaaact gccacctggc gctaatacat cacgacacat
    36661 ttctagcgcc agttccacca gatacatggc tcgggggata tccaccgccg gtgttccgct
    36721 catgtttggt gccatatcgg acatgacaac ctggactttg ctgtcgccaa cgcgctccag
    36781 cagtgctttc atcaccagtt catcacgaaa atcgccctga agaaagtcca caccaacgat
    36841 aggatccata ggtaaaagat cgcaagcgat gatgcggcct ttgccgccaa tttgggtgac
    36901 cacatattgt gaccaaccac ccggagcagc accaaggtcg acaaccgtca ttcccggttt
    36961 aaagagtttg tcactttgct gtatttcatc aagtttaaac caggcacggg aacgtaatcc
    37021 ctttttctgt gcctgttgaa catatttatc gctaaagtgt tcctgaagcc agcggctgga
    37081 gctggcagaa cgcttcttac ctgtcattta actttcccat ggggataact catcgtaacc
    37141 aattgcgtaa atttttacgc gcctatttgg tgatatatgg gagatggcgg tagaatgacc
    37201 cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa caaaaacagc
    37261 acctgaaagg tctggcacat ccgctcaagc cagttgttct gcttggcagt aatggtttga
    37321 ccgaaggggt gctggccgag attgaacaag cgttagagca ccatgaactc atcaaggtga
    37381 aaatcgccac cgaagatcgc gaaacaaaaa ccttgatcgt ggaagctatc gtgcgcgaaa
    37441 ccggcgcctg taatgtacag gtcatcggta aaacgctggt gctttatcgc ccaactaaag
    37501 aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt tacacacatt tgctgtgtaa
    37561 aacgaggggt tttccgcagg caggagagca aaatgccacg ctctgttcgt tgataaaagg
    37621 ccgcagagcg gcctttttcc tttctttaca atacatcaac atcttgagta ttgggtaatt
    37681 cttacaggta ttccacctta attacttcaa attctacttc gccgcccggc gttttgatga
    37741 ccacaacatc atcttcttct ttgccgatca ggccacgagc aataggcgag tttacagaaa
    37801 tcaggttttg tttaaagtca gcttcgtcat cgccaacgat gcgataagtc tgttcttcgt
    37861 cagaatccag attcagcacc gttacggtag caccaaaaat aacgcgccca ttgttgggca
    37921 ttttggtgac atcaatcacc tgcgcgtttg acagcttggc ttcgatgtct ttgatacggc
    37981 cttcgcagaa gccctgctgt tcacgagctg cgtggtattc ggcgttttct ttcaggtcgc
    38041 catgctcacg cgcttccgcg atagcagcaa tgatttcagg acggcgcaca gatttcagaa
    38101 aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata gcttgcattt
    38161 gttatacctc ttgaatattc ctgatggggc aagtcttcac ccaccccggc tgttcggcct
    38221 gcccggcata actcctcacc gggaccagaa gcaaaaaaat accgacccgg gtacaagtcc
    38281 caggtcagct acaattcaca ttttgatggt cattttaccc tgaagttccc gaagggtcat
    38341 cgtttacttt atagggcgtt gcgccgtagt atgacggctc gattccaggt tgttagcgcg
    38401 agattatgcg attttccaga tttatcatcg gattgaccag ctgtatagcg ttcagtgttc
    38461 aggccgcgaa tgttgatgag tacgttactc aactccccgc aggtgccaac cttgccctga
    38521 tggtgcaaaa agtcggtgcg tcggcccccg ctattgatta ccacagtcag cagatggcgc
    38581 tgcccgccag tacccagaaa gtgattactg cgctggcggc gttgattcaa ctcggccccg
    38641 attttcgttt caccacgacg cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg
    38701 gtgacttagt ggcgcgattt ggtgccgatc cgacgttaaa acgtcaggat attcgcaata
    38761 tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg ttgatagata
    38821 cctccatttt cgccagccac gataaagccc ccggctggcc atggaatgac atgacacaat
    38881 gctttagcgc tccgcctgct gccgccatag ttgaccgcaa ctgtttctcc gtctcgctct
    38941 acagtgcccc aaagcctggt gatatggctt ttatacgcgt ggcatcttat taccccgtta
    39001 cgatgttcag ccaggtacgc accctccccc gtggttctgc cgaagcgcaa tactgcgaac
    39061 tggatgtggt gccaagcgac ctgaaccgct ttacgctgac gggatgcctg ccacaacgtt
    39121 ctgagccgct cccgttggct tttgccgtgc aggatggagc cagctatgcc ggtgcaattc
    39181 tgaaagatga gttaaaacag gcgggtatca cctggagcgg aacactgctg cgccagactc
    39241 aggttaacga acctggaacg gtagttgcca gtaaacagtc ggccccgctg cacgatctgc
    39301 ttaagattat gctgaaaaag tcggacaaca tgatcgccga tacggttttc cgcatgatcg
    39361 gccatgcgcg cttcaatgtg cctggaacat ggcgggctgg gtcggacgcc gtgcgtcaga
    39421 tcctgcgcca gcaagccggt gtcgatattg gtaacaccat tattgccgat ggttcagggc
    39481 tttcgcggca taacctgatt gcccccgcca ccatgatgca ggtgctgcaa tacattgccc
    39541 aacacgacaa tgaacttaac tttatctcca tgctgccgct tgcgggctat gacggctctt
    39601 tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg caaagtctca gcgaaaaccg
    39661 gttcgttgca gggggtatat aacctggcgg gattcattac cacagcgagc gggcaacgaa
    39721 tggcatttgt acaatatctt tctggctatg cagtaaaacc cgcggatcag cgtaatcgcc
    39781 gtattccgtt agtgcgtttt gaaagccgtt tgtacaaaga tatttatcag aacaattagt
    39841 caaaaagaaa ccccggcgca tggccggggt ttcagattat tgacaaagtg cgctttgttt
    39901 atgccggatg cggcgtgaac gccttatccg gcctacaaaa tcgtgcaaat tcaatatatt
    39961 gcaatcatct tgtaggcctg ataagtgtag cgcatcaggc ttatttggca ttggtcatta
    40021 gtgattaacg cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc cagtcgtcat
    40081 cccagtcttc atcatcctct tcagcaatct cttcaagctg ctgacggtga taatcatccc
    40141 acatgaattc gactttctct ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa
    40201 tgataaaggt catcacatcc cagcagagat ctttcacgcc cagtccgctc gccgcagaga
    40261 tcagataata tttatcttcc cagcccagcg cttcagcgat cgctttcgct ttctcttcgg
    40321 cttctgcctt atccagcaga tcgatcttgt tgaagactaa ccaacgcggt ttcgccgcca
    40381 gatcctggct gtatttttcc agctcgctga taataatacg cgcgttttca accggatcgg
    40441 tgccgtcaat cggatcgata tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct
    40501 tcaggaagcg aatgcccaga cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat
    40561 cggcaacaac gaagctcttt tcgttgtcca ttcgtaccac acccagactt ggcaccagag
    40621 tggtaaacgg ataatccgcc actttcggtt tagccgccga taccgcacga ataaaggtcg
    40681 atttacccgc gtttggcatc cccaacatac cgacgtcagc cagcagcatc agctccagca
    40741 gcagctcacg cttatcgccc ggcgtaccgt tagttttctg ccgtggtgta cggttaactg
    40801 acgatttgaa acgggtattg cccagaccgt gccagccgcc cttagcaacc agcagacgct
    40861 gaccgtgttt agtcatatca cccatggttt caccagtacc ctggtcgatt acacgcgtac
    40921 ctaccggcac tttaatcgtc acgtctttac cgcgcttacc ggtacaatcg cggcttgcgc
    40981 cgttctgacc gcgctctgca cggaaagatt tttcaaaacg ataatcgata agcgtgttca
    41041 ggttctcgtc ggcttccatc catacgtcac caccatcacc gccgtcgccg ccatccgggc
    41101 cgcctttcgg aatatacttt tcgcggcgga agctcacgca accattaccg ccatcacctg
    41161 caacgaccag aatcgatgct tcatcaacaa acttcatttt attctccgta aatcattcgc
    41221 ctgcgcgggg ttgcgatacc accgttgtat gcttacgtaa tccgccccaa atacgatgac
    41281 caatggcgga atacatcgcg cccgcaacca cgacaaacgc accgagataa cctaaaaggt
    41341 ttaacatcgg tctggcgaag aaatcgggcc aggccagtga taaaagatct gaaaaaaaca
    41401 gcgtaaacag tggggtgagc gtgatgatcg cgctcacctg cgctgcctgc cagcgagcca
    41461 tcgcttccgc caaggcgcca tatcctacca aggtattcag tccgcaaaaa attaaacatg
    41521 cgagctgcca gtggctaagc tgcgctatca ctccaggctt tgccagaggg aagagcgcga
    41581 ttgtacataa agtgtacagt aaaaacagga tctgcggtga ggccagccga cgcaataaaa
    41641 ccttttgcgc cacgccataa ctcacccaaa ccgtcgccgc accgacccca aagataactc
    41701 cccaggtgta atcggtgagc tttgtaaata tctcgaccag actggtgtta aaaaacatca
    41761 ccagaccgct caggagcatc aatgccccta caacctgagt gctgcgcatt ttctctttca
    41821 ggataaatac gctggcaacc atcatgccaa ctggcgagag ttgcccaatc acctgcgaag
    41881 cggtcggact caggtattgc aaggatgagc tgaacaggat gaagttccca aacagcccgg
    41941 cggtcgccac tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt aatggcggca
    42001 acctcttctt caccgcaaga atggcaccaa ggccaatact cgccatcaag aaacggtaaa
    42061 acacgattgt cggaggttcc atcacctcca gcacctgctt cattgcgatt ggcaacgccc
    42121 cccagcaaat tgctgtggta agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac
    42181 cgtattccct gcaaagcgca tttgcggatt aacgtcgcac catgcgcatt tttaccggtt
    42241 atcgaatgta aaaagccccg caacgtgttg cggggctttc atccgttacc gggacgcgaa
    42301 aaacttattc agcttcgatg ctgataaatt tacggttttt cgggccttta acttcgaatt
    42361 tcactttacc gtctgcttta gcaaacagag tgtggtcacg accgcaacct acgttagcgc
    42421 cagcgtggaa tttggtacca cgttgacgaa cgatgatgct acccgccaga acggattcgc
    42481 caccgaaacg cttaacgccc aagcgtttag cttctgaatc gcgaccgtta cgtgtggagc
    42541 cgccagcctt tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc tgatgccagt
    42601 aattttcaca tcagtgaacc actgacgatg gccctgctgc ttacgatagt gtttacgacg
    42661 acgaaactta acgattttaa ctttctcgcc acgaccgtga gcaacaactt cagctttgat
    42721 tacgccgcca tcaacgaaag gaacgccgat tttgacttct tcaccgtttg cgatcatcag
    42781 cacttcagcg aactcaacag tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt
    42841 ctgaccttcg cttactcggt gttgtttacc accactttgg aaaaccgcgt acatataaaa
    42901 ctccgcttcc gcgcacacct tttcaatgat tcagagtgcg ctataaatat tcacaatagg
    42961 gcgcgaatat tacgcaaaac gcacgccttt gacaagtgct acagtcaata cacgaagaaa
    43021 aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa ttaccctata
    43081 ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc
    43141 cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg
    43201 ttaatgcggc aatccttgag cagcttaatt ccgacgtcca actgatcaat cagttaggct
    43261 attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac
    43321 gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc
    43381 acacggcgac tctgctacac gacgacgttg tggatgaatc agatatgcgc aggggtaaag
    43441 ctaccgccaa cgccgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata
    43501 cccgcgcgtt ccagatgatg accagcctcg gttcactcaa agtgctggaa gtcatgtcag
    43561 aagctgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg
    43621 acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg
    43681 ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaggaggag aaaggcctgc
    43741 aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca
    43801 atgccgatgg cgaacagtta ggtaaaaacg tcggcgacga tctgaacgaa ggtaaaccga
    43861 cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta
    43921 ccgccatcga acaaggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg
    43981 cttgtggatc tcttgaatgg acgcgtcagc gcgccgagga agaagcagac aaagccatcg
    44041 cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca
    44101 tcgctgttca acgcgatcgt taatcccctc ccttcatccc gcgcagtgcg cgggatgatt
    44161 ccagtaaatt ccataaaaac actaattcag cgctataaca cctgaaaaaa gggcgatgaa
    44221 tctttatata ttctgaatat tcacactctt tacaggaact ttttagcgca atagaccatc
    44281 aggagtatag tgatgcccga cagaagaagt gttctgaatg aaagcgacta acttaaggag
    44341 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcaggtttg
    44401 cgcaagaaag gaacgtcaat ggcggcggaa tctcgcagaa atggtttgag ttcctcaacg
    44461 ctggcaaacg cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg
    44521 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    44581 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    44641 tagccccgcg aacggggctg ccagctctca gacgattact cgcctttcac acgctcaata
    44701 tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    44761 atacgatcaa ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat
    44821 gcacgcagat cggttgccat aacctgtgca ccagaaagtt tttcaacacc atgacaaata
    44881 acggtattgc tttcgatttc ggcgtgcgcg cccatacggc tcagctctgg cacatgcata
    44941 aagcggtttt caaagaccgt ttcggtgatg aacccggtcc cttcagccac caggttcaac
    45001 agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta
    45061 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    45121 tccgctccag catcacgcag tttcgccagc acggcgtcca gagtatctgg ctgcgcgtta
    45181 cggcagataa ttttgccacg agagatcgcc gccgccacca ggaaagtacc ggtttcgata
    45241 cgatccggca gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    45301 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    45361 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg tgccttccgc cagggttgca
    45421 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    45481 ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    45541 aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca
    45601 ggtagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagcgc ccagatagaa
    45661 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    45721 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag tttcattgat
    45781 gtatcgacgt ctttcagttt cgggacgttc tggatctcta ccggttcttc cgccagtagt
    45841 gcggcaaaaa gaataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    45901 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    45961 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    46021 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    46081 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatacc gtcaaacaac
    46141 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    46201 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    46261 agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    46321 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    46381 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    46441 ttcgcgaggg tatacacttt gtcgtttacc ccctgaagcg tcacgttgtt gccctgcttt
    46501 ttcgccagat cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca
    46561 cggctaagat cgatgcaggt aatccccttc accgcctcct cacgcatttc ccaaagcggt
    46621 agcagaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    46681 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    46741 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    46801 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcttgc cagtttcccg
    46861 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cggacggcca ttcgggtcaa
    46921 taatggtaac gcgaataggc acaatggttt tatcgcccag cggctgttct ggcgcaatct
    46981 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    47041 ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca
    47101 gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg
    47161 tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    47221 ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg
    47281 cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt
    47341 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    47401 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    47461 caatgagatc ttccagcacc atcgcagact tggtgtcctg aattgtatcg ccatccttca
    47521 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttctc
    47581 ccagcaggcc ggaagtacga atgctcagcg aactggtatc aggaatgtgg ttataacgtt
    47641 gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca
    47701 cccggcccac aacaacacca ccaatgctga ccggagagcg cgctttcagg ccgccaatgt
    47761 tatcgaacgt cgcataaagc gtgtaggtcg attcagtacg tatggacgtc acgttcgccg
    47821 ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa
    47881 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    47941 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    48001 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    48061 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    48121 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagcgat
    48181 ccgccccaga tccccacggc gacgaagata accgtcaaca gtggtaatga aataacccca
    48241 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    48301 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttaacgccga accagcacgc
    48361 ccggcaaaca acagcgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    48421 agcataccca gactggtttc cgcgctataa gtggtcagaa ccagataacc ttgcagcccc
    48481 aacaccattc cgatgaacac gccagaaact acaataatca gcatcgacag gacgccgaca
    48541 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    48601 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    48661 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    48721 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    48781 ctgacgtacg cgcggatcag gattcgtctg caacgcctgg gcactgccat gagcgacaat
    48841 ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg
    48901 agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag
    48961 tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg
    49021 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    49081 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    49141 atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa
    49201 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttacgcac
    49261 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc
    49321 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    49381 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    49441 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    49501 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    49561 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    49621 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag
    49681 cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa
    49741 ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc
    49801 tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta
    49861 catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag
    49921 ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg
    49981 cgctggttcg tccttttacc gtccattccg atgttctgcg ccgtgaatta cccttaatgt
    50041 tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgaagcgatg
    50101 gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc
    50161 aggggactga cagcctgacc agagagcagc ttgcagagct gccgcgtgac ggcggattgc
    50221 ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat gccagtggcc acgcggatgg
    50281 tggttgataa cgccacggtg ctggcgaatt actttgccat cagcgagttg acgatggctc
    50341 tgacggcaat tgctatcgga accagtctgc cggaactggc aaccgcaata gcgggggttc
    50401 gcaaaggtga aaacgacatt gctgtcggaa atatcattgg cgcaaacatt tttaatattg
    50461 tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga gattgatcca ctggcgtaca
    50521 gtcgtgacta cagcgtgatg ttgctggtga gcattatttt tgcgttgctg tgctggcggc
    50581 gctccccgca accgggccgt ggtgtagggg tattattaac tggcggattt atcgtatggc
    50641 tggcgatgtt gtactggtta tcgccaatac tcgttgaata actggaaacg cattatgtcg
    50701 cacgtagagt tacaaccggg ttttgacttt cagcaagcag gtaaagaagt cctggcgatt
    50761 gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc agaatttcac gcttgcctgt
    50821 gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg ggatggggaa atcggggcac
    50881 atcgggcgca aaatggccgc aacgtttgcc agcaccggta caccttcatt tttcgtccat
    50941 cctagtgaag ccgcgcatgg tgatttaggc atggtcaccc cacaggatgt ggtgattgct
    51001 atctctaact caggtgaatc cagcgaaatc acggccttaa ttccagtgct taagcgtctt
    51061 cacgtaccgt taatctgcat caccggtcgc ccggagagca gcatggcgcg cgccgcagat
    51121 gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt tagggctggc accgaccagc
    51181 agcaccaccg ccacgctggt tatgggcgat gccctcgctg tcgcgctgtt aaaagcacgc
    51241 ggctttactg ctgaagattt tgcgctctca cacccaggcg gcgcactggg tcgtaaactt
    51301 ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga tcccgcatgt taagaaaacg
    51361 gccagtctgc gtgacgcatt gctggaagtt acccgcaaaa atcttggtat gactgtcatt
    51421 tgcgatgaca atatgatgat tgaaggcatc tttaccgacg gtgatttacg ccgtgtcttc
    51481 gatatgggcg tggatgttcg tcagttaagt attgccgatg tgatgacgcc ggggggaata
    51541 cgtgtgcgcc ctggcattct ggccgttgag gcactgaact taatgcagtc ccgccatatc
    51601 acctccgtga tggttgccga tggcgaccat ttactcggtg tgttacatat gcatgattta
    51661 ctgcgtgcag gcgtagtgta aagattcaag gataaacaac aatgagcaaa gcaggtgcgt
    51721 cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat ggcaaaagca gagaacattc
    51781 gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg cctgatttat atgggcaata
    51841 acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta tggcattcgt tgtgcgctca
    51901 cctctgatat tgaagtcgct atcattaccg ggcgaaaggc taaactggta gaagatcgtt
    51961 gtgccacatt ggggatcact cacttgtatc aggggcagtc aaacaaactg atcgccttta
    52021 gcgatttgct ggaaaaattg gcgattgccc cggaaaatgt ggcttatgtt ggcgatgatc
    52081 tcatcgactg gccggtaatg gaaaaagtgg gtttaagcgt cgccgtggcc gatgcgcatc
    52141 cactgttgat cccgcgcgcc gattacgtga cgcgcattgc tggcggtcgt ggcgcagtgc
    52201 gcgaagtttg cgacttatta ctcctggcgc agggcaaact ggatgaagcc aaagggcaat
    52261 cgatatgagt aaagccagac gttgggttat cattgtgcta tcactggcgg ttctggtgat
    52321 gatcggcatt aatatggccg aaaaagacga taccgcccag gtggtcgtca acaacaatga
    52381 tcccacctat aaaagcgagc atacggacac gctcgtctat aacccagaag gggcactaag
    52441 ctatcgattg attgctcaac acgttgaata ttattccgat caggccgttt cgtggtttac
    52501 gcagccggta cttaccacgt ttgataagga taaaatcccg acatggtccg taaaagcaga
    52561 taaagccaag ctgaccaatg accggatgct ctatttatat ggacacgttg aagtcaacgc
    52621 acttgtgcca gactctcaac ttcgcagaat cacgacggat aacgcgcaga tcaatctggt
    52681 gacgcaggat gttacctctg aagacctcgt cacgttatac ggaacaacat ttaactccag
    52741 cggtctgaaa atgcgcggca acttacgcag caagaacgcc gagctgattg aaaaggttag
    52801 aacatcctat gaaattcaaa acaaacaaac tcagccttaa tcttgtgctt gccagctcac
    52861 ttctggccgc cagcattccg gcatttgccg taaccggaga cactgatcag ccgatccaca
    52921 ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt ggttaccttt accggtaatg
    52981 tcatcgtcac ccagggcacc atcaaaatta atgccgacaa agtggtcgtt acccgtccgg
    53041 gcggcgaaca aggtaaagaa gtgattgacg gatacggtaa accggcaacg ttctaccaga
    53101 tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca gatgcactac gaactggcaa
    53161 aagattttgt cgttctgacg ggtaatgctt atctgcagca ggtcgatagc aacattaagg
    53221 gcgataagat cacttacctg gtgaaagagc agaaaatgca ggctttcagc gacaaaggca
    53281 agcgcgtaac caccgttctg gtgccgtcgc agctgcagga caaaaacaac aaaggccaga
    53341 ccccggcaca gaagaagggt aattaattcg ttatggcaac attaactgca aagaaccttg
    53401 caaaagccta taaaggccgt cgcgtggtag aagacgtcag cctgaccgtc aactccgggg
    53461 aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac caccactttc tacatggttg
    53521 taggcattgt gccgcgcgat gcgggcaaca tcattattga tgatgacgat atcagtctgc
    53581 tgcctctgca tgcacgcgcg cgccgcggta tcggctatct gccacaggaa gcctccattt
    53641 tccgtcgcct cagcgtttac gataacctga tggcggtact gcaaattcgt gacgacttgt
    53701 ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga agagtttcac attgagcacc
    53761 tgcgtgacag catggggcag tcactctccg ggggtgaacg tcgccgtgta gaaattgccc
    53821 gcgcactggc tgcgaatccg aaatttattc tgctcgacga accgtttgcc ggggttgacc
    53881 cgatctcggt tatcgacatt aaacgcatca ttgaacacct gcgcgacagc ggcctgggcg
    53941 tgctgatcac tgaccacaac gtgcgtgaaa cactggcggt ttgtgaacgc gcttatatcg
    54001 tcagtcaggg gcatttgatc gcccacggca cgcctacaga aatcttacaa gacgaacacg
    54061 ttaagcgtgt ataccttggg gaagacttca gactctgata gggtagaagt ttgcgacgtt
    54121 ttagcaggag agtacgattc tgaacatgaa gcaaggtttg caactcaggc ttagccaaca
    54181 actggcgatg acgccacagc tccaacaggc aattcgtctg ttgcagttgt cgacgctgga
    54241 acttcagcag gagctacagc aggcgctgga gagtaatccg ctgcttgagc aaatcgacac
    54301 tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa acgctggaca ccgccgacgc
    54361 gctcgaacaa aaagagatgc cggaagagct gccgctcgat gccagttggg acaccattta
    54421 caccgctggt acaccatccg gcaccagcgg tgactacatt gacgacgagc tgccggtcta
    54481 ccagggcgaa acgacgcaga ccttgcagga ttacctgatg tggcaggtcg agctgacacc
    54541 gttttccgac actgaccgcg ctattgctac ctctatcgtc gatgccgttg atgaaaccgg
    54601 ttatctgact gtcccgctgg aagatattct cgaaagtatg ggcgatgaag agatcgacat
    54661 cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt gacccggtcg gtgtagcggc
    54721 aaaagatctg cgtgactgcc tgctaatcca actctcccaa ttcgataaga ccacgccgtg
    54781 gctggaagag gccagattga tcattagcga tcatctcgat ctgttagcca atcacgactt
    54841 ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg ctgaaagaag ccgtcaatct
    54901 gatccagtcg ctcgatccgc gccccgggca gtcgatccag actggcgaac ctgagtatgt
    54961 cattccagat gtgctggtgc gtaagcataa cggtcactgg acggtagaac tcaacagtga
    55021 cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg atgtgcaata acgcgcgcaa
    55081 cgatggtgac agccagttta tccgcagcaa tctgcaggat gccaaatggt tgatcaagag
    55141 tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc tgtatcgttg aacagcagca
    55201 agccttcttt gagcaaggtg aagaatatat gaaaccgatg gtactggccg atatcgccca
    55261 ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc acgcaaaaat acctgcatag
    55321 tccacgaggc atttttgaac tgaagtattt cttttccagt cacgtcaata ccgagggcgg
    55381 cggcgaagct tcctccacgg cgattcgtgc actggtgaag aaattaatcg cggcggaaaa
    55441 cccagcgaaa ccgttgagcg acagcaagtt aacctctttg ctgtcggaac aaggtatcat
    55501 ggtggcacgc cgcactgttg cgaagtaccg agagtcttta tccattccgc cgtcaaacca
    55561 gcgtaaacag ctcgtttgac ccaaccgata aggaagacac tatgcagctc aacattaccg
    55621 gaaataacgt cgagatcacc gaggcactgc gcgaatttgt tacagctaaa tttgccaaac
    55681 ttgagcaata ttttgaccga atcaaccagg tctatgttgt tctgaaagtg gagaaagtca
    55741 cccacacctc agatgcaaca ctgcatgtaa acggtggtga aattcatgcc agcgcggaag
    55801 gtcaggacat gtacgctgcc attgatggct taattgataa gctggcacgc cagttgacca
    55861 aacataaaga taaactgaaa caacactaat tgtccgggca attagcatgt gcatggcggc
    55921 ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct ccgagcctgt tccactgttt
    55981 gagtgggcag gttcttaggt gaaattatga caaataatga tacaactcta cagcttagca
    56041 gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg tcagagcaaa aaacgcgccc
    56101 tggaaatcat cagcgagctg gcggcgaaac aacttagcct gccgcctcag gtggtttttg
    56161 aagctatcct gacgcgggaa aaaatgggca gtaccggtat cggcaatggt attgccattc
    56221 cgcatggcaa acttgaagaa gatactctgc gtgccgttgg cgttttcgtt cagctcgaaa
    56281 cgcctatagc tttcgatgcc atcgacaacc aaccggtgga cttacttttt gccctgctgg
    56341 tgccggcaga ccaaactaaa acgcacctgc atactctgtc gctggtggcg aaacgtctgg
    56401 cggacaaaac catctgccgc cgtttacgcg cagcccagag cgatgaagag ctgtatcaaa
    56461 tcattacgga taccgaaggt actccggatg aagcgtagtt attcggtaat gtctctttta
    56521 gacgttgtga ggagaaacag tacatggtac tgatgatcgt cagcggacgt tcaggttcag
    56581 gtaaatctgt cgccctgcgt gcgctggaag atatgggttt ttactgcgtg gataaccttc
    56641 ccgtagtgtt gttacccgat ctggctcgaa ctctggccga tcgcgagatt tctgccgccg
    56701 tcagcattga tgttcgtaat atgccggagt caccagaaat attcgaacag gcgatgagta
    56761 acctgcctga cgctttctca ccgcaactac tgttcctgga tgccgaccgt aataccttaa
    56821 ttcgtcgtta cagtgacacg cgccgactgc atccgctttc cagcaaaaac ctgtcgctgg
    56881 aaagtgctat cgacaaagaa agcgatttgc tggagcctct gcgttcgcga gcggatctga
    56941 ttgtcgatac ctcagaaatg tccgttcacg agctggctga aatgctgcgt acccgtctgc
    57001 tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc tttcggcttc aaacacggta
    57061 tccctatcga tgcagattac gtctttgacg tgcgcttctt gccgaacccg cactgggatc
    57121 cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc cgcgttcctc gaccgccaca
    57181 cagaagtaca caattttatc taccagacgc gaagctatct tgagctatgg ttacctatgc
    57241 tggaaaccaa caaccgtagc tacctgacgg tcgccattgg ttgtaccggc gggaagcacc
    57301 gttcggtgta tattgcagag caactggcag actacttccg ctcgcgcggt aaaaacgtcc
    57361 agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc gtcaagcaaa ctgttgaaat
    57421 cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag ctgtttgaat taatgcaggg
    57481 ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc gaggctgaag ccaacagcgt
    57541 tattgcgctg ctgatgttgg attctgccaa aggacggcag attgaagttg aagcgaccgg
    57601 tccacaggaa gaggaagcac tggccgccgt tatcgccctc tttaattctg gttttgatga
    57661 agattaatct tcatcacttt gacatacaac tcccttcaag ctcccccgac aataagaaaa
    57721 tcacgtactg aaatcgttct caatcaacgt catttgtaca ttttgtgcgc ttttcacttt
    57781 cagaagaacc ttaagaaaac cttaagaggc attgtttagg ttttgtttaa gttaatcgac
    57841 catactggag atcgtcagaa aatatttcca ggagatggca tgattcgctt atctgaacaa
    57901 agtcccctgg gcaccggaag acatcggaaa tgttatgcgc atccggaaga tgcccaacgc
    57961 tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata aagagatccg ccgtgagtta
    58021 aagtactacg cgcatcttgg tcgccggtta aaagactgga gtggaatacc gcgctatcac
    58081 ggtaccgttg agacggattg cggaaccggt tatgtctacg atgtgattgc cgattttgac
    58141 ggcaagcctt ccattacgct gaccgaattt gctgaacaat gccgttacga agaagacatt
    58201 gcacaactgc gccagctact gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg
    58261 acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca tcagtgagtc agaggttatc
    58321 ccggtcgtct gcgacaacat tggtgaaagc acattgattc cactggcgac ctggtcgaag
    58381 tggtgttgtc tgcgtaagca agagcggtta tggaaacgat ttatcgccca accggcgctg
    58441 gctattgccc tgcagaaaga tctgcaacct cgtgagagca aaacgctggc cctcacttcg
    58501 cgcgaagctt aatccagctg gtgttgttgc ataaacggct caccgcctaa ctgatacatc
    58561 tgccgtaaaa tccacgcctg acggctacgc acgtagcccg atggcgagga gactttgaaa
    58621 cgaagtggat taggtaatac agctgccagt aatgcagctt ccgaccgggt aagtttgctc
    58681 gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat
    58741 tcggcgatat tcaggtaaac cgtcaggata cgctttttgc tccagactgt ttctatcccc
    58801 agcgttaatc cagcttccag cccttttcgt acccagctac gcccatccca taaaaagaga
    58861 ttttggccgt ctgctgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcgt
    58921 tgtgcgccag ggctttctca atggaagcga catcaaagcc ccagtgctca ggaaatttct
    58981 gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc
    59041 agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc tgtcgctcga
    59101 ccattaccgc tgagaaggga acaggcgcaa cgctaaacaa cgcgatgccc ccgccccaga
    59161 aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta aacaccgtta
    59221 agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct
    59281 cgccgcttct gcaatgttct gcgccagcat atatgctggg gtggtgacaa tcttattgtc
    59341 ttcatcaacc acgatatcat cgacaggaca cggcacatgc tccgcgccca tctcttccag
    59401 cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt
    59461 cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct gatgcatcgc
    59521 ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg ctaccaagac tggcaaaatt
    59581 gcttaaattc ttcgccgcgc caaacccccc cggcacaatc aacgcatcca gttcagcggc
    59641 atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac
    59701 attgcgcgtt tccgtcatcg cttcgccagt taaatggttg ataacatcaa cctgctgctt
    59761 atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac
    59821 cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt
    59881 cttcattgtg atcatccttt cgcaattgac tgaaacacat taattttttt aataaaaatg
    59941 gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac
    60001 tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa tttacggcgc
    60061 agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt
    60121 ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc cactcttctt
    60181 tcatctcttc aatccattca ccgacgttat cttcccaggc gggaaggtca ggagactgaa
    60241 tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca cctttaattt
    60301 tatgtccttc ctcaacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgc
    60361 tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc
    60421 ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt
    60481 tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca
    60541 tcgcggttag cgccggaacc gaaagcggct tactcagcac atcatccatt ccagcattga
    60601 ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct
    60661 cgcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata tctggcaact
    60721 gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc
    60781 cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc gcgacaatca
    60841 cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt
    60901 catcaaacgc atcatcgacc tcttctgcca ccgacggtgc gtggatcgtc aacgtaaagg
    60961 ttgaaccttt gccctgttca ctggtaaccg taatatcgcc gcccatattt ttcgccagac
    61021 gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt
    61081 tcacctggta atacatggcg aagattttat ccagctcatc ctgcggaatg ccgataccag
    61141 aatcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct
    61201 ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc tgccgtaaac
    61261 gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt aggctccagg ttaaagcgca
    61321 atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga
    61381 aatcaaccgg ctgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa
    61441 tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatagttttg agatattttt
    61501 cctgctcggc ggtgagttcg gtatccagca gaatgcggct caggccgacg ataccgttca
    61561 gcggtgtacg caattcgtga ctgatggtgg aaataaacgt cgttttgtcg cggctggcgc
    61621 gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac
    61681 cgtgacgttt acccacgcgg tcgtagtacg gcactttacg gatttcaaag caggctttgc
    61741 gcccgtccgg gtaatccagc cactgttcat aggtcagtga cacattatga cggaacactt
    61801 tttcatcggt ttcaatgact tttgcggcgg cttccggtga gtaaacatca gcaggtttca
    61861 ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg
    61921 aaaactcttt atcttcgtta cgataaaaaa ccaagtcggg tgaagcatca aggaaggaac
    61981 gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa
    62041 ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcaattt
    62101 cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc
    62161 gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc tccagttgct
    62221 cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag
    62281 aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc gccatttgta
    62341 ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta
    62401 acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat
    62461 tccttcacga caacctgccc caataatacc caattctgac agttacgtta taaattgtgt
    62521 gagaaatgcg gaattcccct caacgaccta ctccgttgga ggtagccacg gacgtcccag
    62581 cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca ttcccgtcca
    62641 tcgattttcg caccacagcg gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg
    62701 gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtataatc
    62761 ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt
    62821 cacaatatgc agcggatgca gctttatgcc gtccacgctg gtttcaacca ctcgctccag
    62881 cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt gggaacacac
    62941 cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa aatcatggcc
    63001 gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct ccagccacac
    63061 ttcgtaaccc tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc
    63121 cgggcgggta ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat
    63181 agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt aggccagata
    63241 gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg
    63301 ctgctgcgct tcatcggcaa acgaggcaac attacagaat gtgcagccac cacgcccgat
    63361 ggtaccgtca cggttagggc agctaaaacc gccgtgcagc gtcagcttat gcaccttttg
    63421 cccataacga cgggtgagat caccaccaaa catattgact aatttctgta actgcataat
    63481 ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca attcggcgat
    63541 gacctggatc aatcgtcccg gcctgctttt atcaactgca taatcaatca aaattaccga
    63601 aatttcatgc acaatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc
    63661 cttatggatt ttaggcaaaa acagtagcat gaaacgtcat taccaattaa ggcagtataa
    63721 aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt tatgacagtc
    63781 aggaattgac tgtttctcta acgactctcc ttttagcctt aaagataaaa tctattttaa
    63841 tttcagctat ttaataaaga attttgcgct aaagcacatt tctgtaccaa taagcttgcc
    63901 atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag
    63961 cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg
    64021 cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc
    64081 acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg
    64141 aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga
    64201 tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg
    64261 cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac ggttgcggct
    64321 tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt
    64381 tagcaaaaaa ctacgctgtc gggatgctct tcctgaataa agatcctgaa ctcgccgctg
    64441 ccgcacgccg catcgttgaa gaagaactgc aacgcgaaac cttgtcgatt gtgggctggc
    64501 gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccacgca
    64561 ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc acgcgatatg gagcgccgtc
    64621 tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct
    64681 gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg gatctgccgc
    64741 gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catttgcctg ttccaccagc
    64801 gcttctccac taacaccgta ccgcgctggc cgctggcgca accgttccgc tatctggcgc
    64861 ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg cgtacctata
    64921 aattccagac gccgctgatc cctgacctgc acgacgccgc gccattcgtc aacgaaaccg
    64981 gttctgactc cagttccatg gataacatgc tggaactgct gctggcaggc gggatggata
    65041 tcatccgcgc catgcgcctg ttagtgccac ccgcctggca gaacaacccg gatatggacc
    65101 cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggtccgg
    65161 cgggcatcgt gatgtccgat ggtcgcttcg ctgcctgtaa cctcgaccgt aacggtctgc
    65221 gtccggcacg ctacgtcatt accaaagaca agctcatcac ctgcgcctct gaagtcggta
    65281 tctgggatta ccagcctgac gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga
    65341 tggtgatcga cactcgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga
    65401 aaagccgcca cccttataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg
    65461 aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg cttgccagct
    65521 accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc gtactgggcg
    65581 aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc gtgctctcca
    65641 gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg actaacccgc
    65701 caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa
    65761 tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa tcgccgattc
    65821 tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac cgcgcagata
    65881 cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacagtc aaagagctat
    65941 gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgtt gctggtgctc tccgaccgga
    66001 atatcgctaa agatcgcctg ccggttccgg ctccgatggc ggttggcgcg atccagaccc
    66061 gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc
    66121 gcgatccgca ccacttcgcc gtgttgctgg gtttcggcgc gacggctatt tatccgtacc
    66181 ttgcctatga aacgctgggc cgcctggtag acacccacgc gattgccaaa gattatcgta
    66241 ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa
    66301 tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctac
    66361 acgatgatgt agtgggcctg tgcttccagg gcgcggtcag ccgcattggt ggggcaagct
    66421 ttgaagactt ccagcaggat ctgctgaatc tgtcgaaacg tgcctggctg gcgcgcaagc
    66481 cgattagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac gcctacaacc
    66541 cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg cgagtacagc gactatcagg
    66601 aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta
    66661 cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac
    66721 gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg
    66781 cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc gaagacccgg
    66841 cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg
    66901 ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga
    66961 agccgggcga aggtggtcag ttgccgggcg acaaagtcac tccttacatc gccaaactgc
    67021 gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat atctactcta
    67081 tcgaagactt agcgcagctt attttcgacc tcaagcaggt taacccgaaa gcgatgatct
    67141 ccgttaagct ggtgtccgaa ccaggcgttg gcaccatcgc gactggcgtg gcgaaagctt
    67201 atgcggactt gatcaccatc gcgggctatg acggcggcac cggcgcaagc ccgctgtcgt
    67261 cagtgaaata tgcaggctgc ccgtgggaac tcggtctggt ggaaacccaa caggcgctgg
    67321 ttgccaacgg cttgcgtcat aaaattcgtt tgcaggttga tggcggcctg aaaacgggtg
    67381 tcgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc actggcccaa
    67441 tggtggcgct cggctgtaaa tatctgcgta tttgccacct gaacaactgc gccacgggtg
    67501 tagcaactca ggatgacaaa ctgcgtaaga atcactatca cggcctgccg ttcaaggtga
    67561 ctaattactt tgagtttatc gcccgtgaaa cccgcgagct gatggcacag ctgggagtaa
    67621 cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agagctggac ggtttcaccg
    67681 ccaaacagca gaaactggcg ctgtcgaagc tgctggagac tgccgagcca catgcaggta
    67741 agtcactcta ctgcactgaa aacaacccgc cgtttgataa cggcctactg aacgcgcagt
    67801 tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag caaaaccttc tggttcgata
    67861 tccgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg
    67921 gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc gcaggccaga
    67981 gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct gaccggggat gccaacgact
    68041 atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggttccgcct
    68101 tccgcagcca tgaagcaagc attatcggca acacctgcct gtatggcgcg accggtggtc
    68161 gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc ggtgctatca
    68221 ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggcggt attgtctgta
    68281 ttctgggtaa aaccggcgtt aacttcggcg cgggcatgac cggcggcttc gcttacgttc
    68341 tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga actggtcgaa gtcttaagcg
    68401 tcgacgatct ggcgatccat gaagagcatc tgcgcggtct tatcaccgag catgttcagc
    68461 ataccggttc tcagcgcggt gaagagattc tggcgaactg gtcaaccttc gccacgaaat
    68521 tcgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcac cgtagtcgta
    68581 gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt cagaatgttt
    68641 atcaatttat cgacctgcag cgcgttgatc cgccaaagaa accgctgaag atccgtaaaa
    68701 ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg caggctgacc
    68761 gctgtctgtc gtgcggcaac ccatactgcg agtggaaatg cccggtacac aactacatcc
    68821 caaactggct gaagctcgcc aacgaggggc gtatttttga agcggcagaa ctgtcgcatc
    68881 agaccaacac actgccggaa gtatgcggac gtgtctgccc gcaagaccgt ctgtgcgaag
    68941 gttcctgcac cctgaacgat gagttcggtg cggtgaccat cggcaacatt gagcgttata
    69001 tcaatgataa agcgttcgaa atgggctggc gtccggatat gtccggcgtg aagcaaaccg
    69061 gtaaaaaagt ggcgattatc ggtgcaggcc cggcaggtct ggcgtgtgcg gatgtcctga
    69121 cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc agaaattggc gggctgctga
    69181 ccttcggtat tccggccttc aagctggaaa aagaggtaat gacgcgtcgc cgtgaaatct
    69241 tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac gtacagctgg
    69301 acgatctgct gagtgattac gatgccgtgt tccttggcgt tgggacttat cagtcaatgc
    69361 gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg ttccttatcg
    69421 ccaacaccaa acagttaatg ggctttggtg aaacccgcga cgaaccgttc gtcagcatgg
    69481 aaggcaaacg cgtggtagta cttggcggtg gcgacactgc gatggactgc gtgcgcacgt
    69541 ctgtacgcca gggggcaaag cacgttacct gtgcctatcg tcgtgatgaa gagaacatgc
    69601 cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc aaattcaacg
    69661 tccagccact ggggattgaa gtgaacggta acggcaaagt cagcggcgta aaaatggtac
    69721 gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc gttgcaggtt
    69781 ccgaacatat cgttccggca gatgcggtga tcatggcgtt tggtttccgt ccacacaaca
    69841 tggaatggct ggcaaaacac agcgtcgagc tggattcaca aggccgcatc atcgctccgg
    69901 aaggcaacga caacgctttc cagaccagca acccgaaaat ctttgctggc ggcgatatcg
    69961 tccgtggttc cgatctggtc gttaccgcca tcgccgaagg tcgcaaagcg gcagacggca
    70021 ttatgaactg gctggaagtt taagct
//
LOCUS       DAJTYH010000005        67013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTYH010000005 DAJTYH010000000
VERSION     DAJTYH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67013
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO609_000331"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO609_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6631875.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO609_000332"
     CDS             complement(1151..1237)
                     /locus_tag="OO609_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6631876.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO609_000333"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO609_000333"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6631877.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO609_000334"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO609_000334"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6631878.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO609_000335"
     CDS             3586..3630
                     /locus_tag="OO609_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631879.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO609_000336"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO609_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6631880.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO609_000337"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO609_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6631881.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO609_000338"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO609_000338"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6631882.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO609_000339"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO609_000339"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6631883.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO609_000340"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO609_000340"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6631884.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO609_000341"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO609_000341"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6631885.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO609_000342"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO609_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6631886.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO609_000343"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO609_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6631887.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO609_000344"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO609_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6631888.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWLEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO609_000345"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO609_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6631889.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO609_000346"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO609_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6631890.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO609_000347"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO609_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6631891.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO609_000348"
     CDS             complement(16981..17745)
                     /locus_tag="OO609_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6631892.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO609_000349"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO609_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6631893.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO609_000350"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO609_000350"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6631894.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO609_000351"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO609_000351"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6631895.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO609_000352"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO609_000352"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6631896.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO609_000353"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO609_000353"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6631897.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO609_000354"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO609_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6631898.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO609_000355"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO609_000355"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6631899.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO609_000356"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO609_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6631900.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO609_000357"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO609_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6631901.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO609_000358"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO609_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6631902.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO609_000359"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO609_000359"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6631903.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO609_000360"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO609_000360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6631904.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO609_000361"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO609_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6631905.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO609_000362"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO609_000362"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6631906.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO609_000363"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO609_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6631907.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO609_000364"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO609_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6631908.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTQALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO609_000365"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO609_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6631909.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO609_000366"
     CDS             complement(40421..41815)
                     /locus_tag="OO609_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6631910.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO609_000367"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO609_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6631911.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO609_000368"
     CDS             complement(42158..43444)
                     /locus_tag="OO609_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6631912.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO609_000369"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO609_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO609_000370"
     CDS             complement(44451..45221)
                     /locus_tag="OO609_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6631913.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="OO609_000371"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="OO609_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6631914.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="OO609_000372"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="OO609_000372"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6631915.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="OO609_000373"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="OO609_000373"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6631916.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="OO609_000374"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="OO609_000374"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6631917.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="OO609_000375"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="OO609_000375"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6631918.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="OO609_000376"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="OO609_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6631919.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO609_000377"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO609_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6631920.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO609_000378"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO609_000378"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6631921.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO609_000379"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO609_000379"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6631922.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO609_000380"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO609_000380"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6631923.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO609_000381"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO609_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6631924.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60319..61269)
                     /gene="ispH"
                     /locus_tag="OO609_000382"
                     /gene_synonym="lytB"
     CDS             complement(60319..61269)
                     /gene="ispH"
                     /locus_tag="OO609_000382"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6631925.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61271..61720)
                     /gene="fkpB"
                     /locus_tag="OO609_000383"
     CDS             complement(61271..61720)
                     /gene="fkpB"
                     /locus_tag="OO609_000383"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6631926.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61807..62301)
                     /gene="lspA"
                     /locus_tag="OO609_000384"
     CDS             complement(61807..62301)
                     /gene="lspA"
                     /locus_tag="OO609_000384"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6631927.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62301..65117)
                     /gene="ileS"
                     /locus_tag="OO609_000385"
     CDS             complement(62301..65117)
                     /gene="ileS"
                     /locus_tag="OO609_000385"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6631928.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65160..66101)
                     /gene="ribF"
                     /locus_tag="OO609_000386"
     CDS             complement(65160..66101)
                     /gene="ribF"
                     /locus_tag="OO609_000386"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6631929.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66109..66327)
                     /locus_tag="OO609_000387"
     CDS             complement(66109..66327)
                     /locus_tag="OO609_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6631930.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66430..66693
                     /gene="rpsT"
                     /locus_tag="OO609_000388"
     CDS             66430..66693
                     /gene="rpsT"
                     /locus_tag="OO609_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6631931.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16256 a        18244 c        17251 g        15262 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccttgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcttga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt aaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgcctgcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgat gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgctgc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttttat gaagaaaaca tgccggtaac aagaccggca ttttcgcata
    60301 acttaggctg ctaatgactt aatcgacttc acgaatatcg acacgcagct ctttcggcac
    60361 ttcgaaaaca atattttctt cacggccttc cagcggaatg gcttcaccac cacccaactg
    60421 ctgcaaacgt gccaccacat tctgcaccag aatatccgga gccgatgcgc ccgcagtcac
    60481 gccgacgcat ttaacctctt tcacccactc ttcctggata tctttcgcat cgtcaatcaa
    60541 aaacgcgtgt ttgcccatac gctgggccag ctccgccaga cggttggagt tggaggagtt
    60601 tttcgaaccg accaccaaca caacttccgc ctgttctgcc agggcgcgta ccgcttcctg
    60661 acggttagtc gtggcgtagc agatgtcatc tttgcgcgga ccgacaattt tcgggaagcg
    60721 tttacgcagc gcgtcgatca catcagacgt gtcatccacc gacagcgtgg tttgggtcat
    60781 aaaggagagc ttctcttcgt ttttgaccgt cagtttccac acatcgtctg gcgattcgac
    60841 tagatacatt cccccttcgg ggttgctgta ctgccccatc gtcccttcca cttccgggtg
    60901 cccggcgtgg ccgatgagaa tagattcttc gccacggcga ctggcgcggg caacttccat
    60961 atgcactttg gtcaccagcg gacaggtggc atcaaacacc gtcaaatcgc ggcttttcgc
    61021 ttcgttacga accgcctgag aaacaccgtg tgcggagaaa atcaggatcg cgccgtccgg
    61081 cacttcgcta atctgctcaa taaagatagc cccacgctcg cgcaagctat cgaccacgta
    61141 gcggttatgc accacttcgt gacggacata tatcggtgcg ccgtaaatgg ccagcgcgtt
    61201 ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa ccacgcgggt tggccaacag
    61261 gatctgcatg ttacgcctcc agtgccggat cgatttccag cacttcaata tcaaaatgaa
    61321 cggtctgccc ggccagcgga tggttgaaat caacggtaat ggagtcgccg ttaatttcgc
    61381 ggatcacacc aggcatctca ctgccatcca ttgcggtaaa aagcatgatt gcgccaattt
    61441 ctggctcgcc tgcatccata aattcacggc gggagaagta ctgaatcagg tccggtgacg
    61501 gcacgccaaa cgccgcatct ggctccagcg agaaggtggt tttgtcgccc actttcagcc
    61561 ccagcaggtg ttgctccagc ccttcagaaa gagaagcatc gcccaggcgg aacagcgccg
    61621 gtttaccgtt gttgcgagta gactcggcgg tggtgccatc gtcgagtttt agcgtgaagt
    61681 gcaccaggac ggcgctattg ctctgtacag attcagacat gcaggtttgc tcttattttt
    61741 gaggattttt gccggatgcg gcctacgatt tcgtagcaat ctgtaggccg catccggcag
    61801 gatttattat tgttttttcg ctttggaagg taaaaaccct tccagcacga tcagtgccgc
    61861 accgacacaa atggcagtat cggcaaggtt gaaggtggcg aagtgccagt cgccgacgta
    61921 gaagtcgatc atatcgacaa cgaagccgtg ccacaggcgg tcgaacaggt tgcccagcgc
    61981 gccgccaata atcagcgcgt aagcgatatt gtttagcttc tgcgtggcct tcgagcgata
    62041 catcatcact gccaggagca cgctaatacc aatcgcaata ccggcaaaga accaacgctg
    62101 ccagccgccg ctatcggcaa ggaaactaaa cgccgcgcca tagttacgcg cataatgcag
    62161 attaagcgac gggaacagcg ggaccgtatc ccccagagca aagttctgga ggatcaggta
    62221 tttgctgccc agatcgataa tcagcacgac taccaccagc cacagccagc gtagccctgt
    62281 tgaacagatc gattgactca tcaggcaaac ttacgttttt caccgtcacc ggcgacgttg
    62341 ctgacacagc ggccgcagat ttctgcgtgt tccgccacct tgccgacatc ctgggtgtag
    62401 tgccagcagc gtgggcactt ctcaccttcg gctttactca acgcgacttt cagccctttg
    62461 agtacttcgc tctgctgagc atcagcaggt gcgtcgttat agtctgcaac ggtagcgccg
    62521 gaggtcaaca ggacaaatcg taattcatcg cccagcgcgg tcagtttcgc cgccagttcc
    62581 ggttctgcat acaaggttac tgccgcttcc agcgagccac ccactttctt gtcggcacgc
    62641 gcttgctcaa tgactttgtt cacttcgcca cgcactttca acagctcgtc ccagaacgca
    62701 tcgttcattg cttcactgtc tgccagacca aacaggcctt cgtaccactc accggtgaag
    62761 acgtattttt cacgttcgcc cggcagatag ccccacactt catcagcggt gaaggagagg
    62821 attggtgcca tccagcgcac cagcgcttca gcgatgtgat acagcgcagt ctggcagcta
    62881 cgacgcgcca cgctatccgc tttggcggtg tactgacggt ctttgatgat gtcgaggtag
    62941 aaggaaccca tctcaacgga gcagaagcgc atcagacgct gtaccacttc gtggaaatcg
    63001 tatgcttcgt acgccttgag gatgtcttcc tgtgccgctt tcgcacaacc tacggcccag
    63061 cgatccagta ccaccatctc ttccggtttc accatatctt ttgctggatc aaaaccattc
    63121 aggtttgcca gcaggaagcg cgcggtgtta cggatacgac gatagctatc ggcagcacgt
    63181 ttcaggatct cgtcagaaac ggccatttca ccggtgtagt cggttgatgc cacccacaga
    63241 cgcagaatat ccgcgcccag tttgttcatc acatcctgcg gcgaaacggt gttgccgatg
    63301 gatttagaca tcttgcgacc ctgaccatcc acggtaaagc cgtgggtcag tacctgacga
    63361 tacggcgctt tgcctttcat cgcggtggag atcattaggg aagacatgaa ccaaccacgg
    63421 tgctggtcag aaccttccag atacatgtcc gctgcgtgac cggcaaattc cggacgcacg
    63481 tcaacaacag aagagtgggt agatccggag tcaaaccata cgtcgagagt atccggcact
    63541 ttcacatact ggtcagcttc gtcgccgagg atctctttcg catcgagatc ccaccacgcc
    63601 tggatgccgt caacttcaac gcgtttagcc acttcttcca tcagttcgag ggtacgcgga
    63661 tgcagctctt ccgtgtcttt gtgcacgaac agtgacatcg gtacgcccca ggtgcgctga
    63721 cgggagatac accagtcagg acggttagca accatcgact cgatacgcgc ctggccccag
    63781 tccgggagcc actgcacgcc tttgatctct ttcagtgact gcgcacgcag acctttctga
    63841 tccatgctga cgaaccactg cggtgtcgcg cggaagatga tcggcgtttt gtgacgccag
    63901 cagcacggat agctgtgctg cattttctca acgtgcagca gcgcgccttt ttcctgcagc
    63961 agcgcaacga cgatgtcgtt cgctttgaag acgttcacgc catccagcgt cggataagtg
    64021 cccggcagat aagtgccgtc cgggccaacc gggttagcgg tttccaggcc gtatttctga
    64081 ccgatcacat agtcgtccgg gccgtggcca ggcgcggtgt gaacggcacc ggtaccggca
    64141 tccagggtaa cgtgatcgcc gaggattgcc ggaacgtcga agcccataaa cggatgggta
    64201 aagcgcagca gctcaagctc tgcacctttt accgtgccga gaatggtgta atcggtcacg
    64261 ccgatacgct gcattacgct ttcaaccaga tctttcgcca gaatcacggc ctgaccgtcg
    64321 atctgcacca gcgcatagtc gaaatcaggt gcaatagaga ttgcgcggtt cgcaggcaga
    64381 gtccacggcg tagtggtcca gatcaccagc gagattgggc cgttaacgtt gcttacgcca
    64441 aatttcgctt tcagcgcatc ctgatcgacc gcctggaaag cgacgtcaat ggacggagaa
    64501 gttttgtcgt aatactcaac ttccgcttct gccagtgcag aacggcagtc tacgcaccag
    64561 tgcaccggct tcgcgccttt gtgcaggtga ccattgccga tgattttgcc cagcgcgcgg
    64621 atgatgttgg cttcagtttt gaagtccata gtcagatacg ggtgcgacca gtcgcccagc
    64681 acgcccagac ggataaagtc tttgcgttga ccgtcaacct gggtcgccgc gtattcgcgg
    64741 cacttggcgc ggaactcggc ggcggtgaat ttctcacccg gcttaccgta ttcttgctct
    64801 actttcagct cgatcggcag accgtggcag tcccagccag gcacatacgg cgagtcataa
    64861 ccggaaagcc ctttggactt cacgataatg tctttcagaa tcttgttaac cgagtgacca
    64921 atatgaatgc tgccattcgc ataaggaggg ccatcatgca gaatgaaggt ttttttgcct
    64981 tttttagccg cacggatgat gccgtacaga tcatcatcag tccaacgcgc cagcattccc
    65041 ggttcgcgct tggcgagatc gccacgcatc gggaaccctg tttccggcaa attcagggtt
    65101 gatttatagt cactcatcag attctcggtt ccgtgtttcg gtttgattac ataacaggct
    65161 taagccggtt ttgttagccc aaaaaattcg cgggcggtta attcatcacg cgcaatctgc
    65221 gctttcagtt cgtccagcga cgcaaatcgc tgctcattgc gtattttttt acgcagcact
    65281 acttgtatat ggcgaccgta aaggtccatt gcaacatcta acaaatgcac ttccagctgc
    65341 tggcgaatac cggcaaccgt tgggcgtgtt ccgatgtttg ccacgccggg taacggcttt
    65401 tcaccgaggc ccagcacttc taccgcataa acccctttca ccggggaaac ctgacggcgt
    65461 agcggtacat tcgccgtcgg gaaacctata gtgcgcccta attcatcacc gtggactaca
    65521 cgcccggaga tggcaaacgg gtgccccagt aaactttctg ccagagccag attgtcatca
    65581 gccagcgcct gacgcacggc ggtgctgctg atacgcacac caccttcgca aaaagtttgc
    65641 gtactggtga tatcgaagcc gtattccatg ccagctttct gtaataacaa gaaatcgcct
    65701 tcacgaccag cgccaaagcg gaaatcatca cctacggcaa gaaattttac gcgcaaatgc
    65761 ttcaccaaaa gatcgctgat gaaattttgc gcggttaacg ccgcaaaacg cctgtcgaaa
    65821 cgcacgcaca gcacgtaatc aacgccacac tctgcaaggt aacgcagttt ttcccgcagc
    65881 cgggtcagtc ttgccggggc tttatcggta gcaaacagtt ccagtggttg aggttcaaaa
    65941 agcatcacca tcaccggtaa gttgcgcttg cgcccttctt cctgcaagcc ctgtaacagc
    66001 gcgcgatgac cgcgatgcac gccgtcgaaa ttaccaatag tcagcacaca cccttcttgc
    66061 ggggcctggc tgagattatg tatgccgcgt atcagcttca tgtctggctc aaaacagtga
    66121 aaatcgtccg agtatacctt gtacagcggt caaggttaac cggcgattga gtaccgaaat
    66181 cagaaagaag tggcttttca ttgccatgga gcaaatcacg ggaagaaact gaccgcctgc
    66241 tgcaattttt atcgcggaaa agctgtattc acaccccgca agctggtaga atcctgcgcc
    66301 atcactacgt aacgagtgcc ggcacattaa cggcgcttat ttgcacaaat ccattgacaa
    66361 aagaaggcta aaagggcata ttcctcggcc tttgaattgt ccatatagaa cacatttggg
    66421 agttggacct tggctaatat caaatcagct aagaagcgcg ccattcagtc tgaaaaggct
    66481 cgtaagcaca acgcaagccg tcgctctatg atgcgtactt tcatcaagaa agtatacgca
    66541 gctatcgaag ctggcgacaa agctgctgca cagaaagcat ttaacgaaat gcaaccgatc
    66601 gtggaccgtc aggctgctaa aggtctgatc cacaaaaaca aagctgcacg tcataaggct
    66661 aacctgactg cacagatcaa caaactggct taatcgccaa tttgctgaag ctttgtgaaa
    66721 aagcccgcgc aagcgggttt ttttatgcct gctgcttttg tgatgcgtga gagaacacac
    66781 tgccggttgc cagatagtca aaagagacat gccgttgtac tatctgaact cttcgtggct
    66841 taagaatcgt agaaccatcg gctgatacat tctgaacaag aatatctact caatctccag
    66901 actcccccac aaagaatatg gatattgtga tacacattga ggtattaaac ttaagaccac
    66961 tctatttttg aattaaattc ctatatactt taaccattca taaatatctc ctc
//
LOCUS       DAJTYH010000006        66847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTYH010000006 DAJTYH010000000
VERSION     DAJTYH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            63..702
                     /locus_tag="OO609_000389"
                     /pseudo
     CDS             63..702
                     /locus_tag="OO609_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            805..1128
                     /locus_tag="OO609_000390"
     CDS             805..1128
                     /locus_tag="OO609_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6631932.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1195..2652)
                     /gene="nuoN"
                     /locus_tag="OO609_000391"
     CDS             complement(1195..2652)
                     /gene="nuoN"
                     /locus_tag="OO609_000391"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6631933.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2659..4188)
                     /gene="nuoM"
                     /locus_tag="OO609_000392"
     CDS             complement(2659..4188)
                     /gene="nuoM"
                     /locus_tag="OO609_000392"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6631934.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4352..6193)
                     /gene="nuoL"
                     /locus_tag="OO609_000393"
     CDS             complement(4352..6193)
                     /gene="nuoL"
                     /locus_tag="OO609_000393"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6631935.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6190..6492)
                     /gene="nuoK"
                     /locus_tag="OO609_000394"
     CDS             complement(6190..6492)
                     /gene="nuoK"
                     /locus_tag="OO609_000394"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6631936.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6489..7043)
                     /gene="nuoJ"
                     /locus_tag="OO609_000395"
     CDS             complement(6489..7043)
                     /gene="nuoJ"
                     /locus_tag="OO609_000395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6631937.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7055..7597)
                     /gene="nuoI"
                     /locus_tag="OO609_000396"
     CDS             complement(7055..7597)
                     /gene="nuoI"
                     /locus_tag="OO609_000396"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6631938.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7612..8589)
                     /gene="nuoH"
                     /locus_tag="OO609_000397"
     CDS             complement(7612..8589)
                     /gene="nuoH"
                     /locus_tag="OO609_000397"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6631939.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8586..11312)
                     /gene="nuoG"
                     /locus_tag="OO609_000398"
     CDS             complement(8586..11312)
                     /gene="nuoG"
                     /locus_tag="OO609_000398"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6631940.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11365..12702)
                     /gene="nuoF"
                     /locus_tag="OO609_000399"
     CDS             complement(11365..12702)
                     /gene="nuoF"
                     /locus_tag="OO609_000399"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6631941.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12699..13199)
                     /gene="nuoE"
                     /locus_tag="OO609_000400"
     CDS             complement(12699..13199)
                     /gene="nuoE"
                     /locus_tag="OO609_000400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6631942.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13202..15004)
                     /gene="nuoC"
                     /locus_tag="OO609_000401"
     CDS             complement(13202..15004)
                     /gene="nuoC"
                     /locus_tag="OO609_000401"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6631943.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15098..15760)
                     /gene="nuoB"
                     /locus_tag="OO609_000402"
     CDS             complement(15098..15760)
                     /gene="nuoB"
                     /locus_tag="OO609_000402"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6631944.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15776..16219)
                     /gene="nuoA"
                     /locus_tag="OO609_000403"
     CDS             complement(15776..16219)
                     /gene="nuoA"
                     /locus_tag="OO609_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6631945.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16850..17788)
                     /gene="lrhA"
                     /locus_tag="OO609_000404"
     CDS             complement(16850..17788)
                     /gene="lrhA"
                     /locus_tag="OO609_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6631946.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18707..19924
                     /gene="alaA"
                     /locus_tag="OO609_000405"
     CDS             18707..19924
                     /gene="alaA"
                     /locus_tag="OO609_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6631947.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20008..20607
                     /gene="yfbR"
                     /locus_tag="OO609_000406"
     CDS             20008..20607
                     /gene="yfbR"
                     /locus_tag="OO609_000406"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6631948.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20666..22498)
                     /locus_tag="OO609_000407"
     CDS             complement(20666..22498)
                     /locus_tag="OO609_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6631949.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22585..23235)
                     /gene="hxpA"
                     /locus_tag="OO609_000408"
     CDS             complement(22585..23235)
                     /gene="hxpA"
                     /locus_tag="OO609_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6631950.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23246..23740)
                     /locus_tag="OO609_000409"
     CDS             complement(23246..23740)
                     /locus_tag="OO609_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6631951.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23823..24278)
                     /locus_tag="OO609_000410"
     CDS             complement(23823..24278)
                     /locus_tag="OO609_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6631952.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24616..25818
                     /gene="ackA"
                     /locus_tag="OO609_000411"
     CDS             24616..25818
                     /gene="ackA"
                     /locus_tag="OO609_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6631953.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25893..28037
                     /gene="pta"
                     /locus_tag="OO609_000412"
     CDS             25893..28037
                     /gene="pta"
                     /locus_tag="OO609_000412"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6631954.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28227..29747
                     /gene="yfcC"
                     /locus_tag="OO609_000413"
     CDS             28227..29747
                     /gene="yfcC"
                     /locus_tag="OO609_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6631955.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29780..30322)
                     /gene="yfcD"
                     /locus_tag="OO609_000414"
     CDS             complement(29780..30322)
                     /gene="yfcD"
                     /locus_tag="OO609_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6631956.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30380..30931)
                     /gene="yfcE"
                     /locus_tag="OO609_000415"
     CDS             complement(30380..30931)
                     /gene="yfcE"
                     /locus_tag="OO609_000415"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6631957.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30987..31631)
                     /gene="yfcF"
                     /locus_tag="OO609_000416"
     CDS             complement(30987..31631)
                     /gene="yfcF"
                     /locus_tag="OO609_000416"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6631958.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31767..32414
                     /gene="yfcG"
                     /locus_tag="OO609_000417"
     CDS             31767..32414
                     /gene="yfcG"
                     /locus_tag="OO609_000417"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6631959.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32471..32833
                     /gene="folX"
                     /locus_tag="OO609_000418"
     CDS             32471..32833
                     /gene="folX"
                     /locus_tag="OO609_000418"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6631960.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32854..33747
                     /locus_tag="OO609_000419"
     CDS             32854..33747
                     /locus_tag="OO609_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6631961.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33795..34685)
                     /gene="rpnB"
                     /locus_tag="OO609_000420"
     CDS             complement(33795..34685)
                     /gene="rpnB"
                     /locus_tag="OO609_000420"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6631962.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34882..35655)
                     /gene="hisP"
                     /locus_tag="OO609_000421"
     CDS             complement(34882..35655)
                     /gene="hisP"
                     /locus_tag="OO609_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6631963.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35663..36379)
                     /locus_tag="OO609_000422"
     CDS             complement(35663..36379)
                     /locus_tag="OO609_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6631964.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36376..37062)
                     /gene="hisQ"
                     /locus_tag="OO609_000423"
     CDS             complement(36376..37062)
                     /gene="hisQ"
                     /locus_tag="OO609_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6631965.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37152..37934)
                     /gene="hisJ"
                     /locus_tag="OO609_000424"
     CDS             complement(37152..37934)
                     /gene="hisJ"
                     /locus_tag="OO609_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6631966.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38155..38937)
                     /gene="argT"
                     /locus_tag="OO609_000425"
     CDS             complement(38155..38937)
                     /gene="argT"
                     /locus_tag="OO609_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6631967.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39203..39772)
                     /gene="ubiX"
                     /locus_tag="OO609_000426"
     CDS             complement(39203..39772)
                     /gene="ubiX"
                     /locus_tag="OO609_000426"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6631968.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39867..41384)
                     /gene="purF"
                     /locus_tag="OO609_000427"
     CDS             complement(39867..41384)
                     /gene="purF"
                     /locus_tag="OO609_000427"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6631969.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41421..41909)
                     /gene="cvpA"
                     /locus_tag="OO609_000428"
     CDS             complement(41421..41909)
                     /gene="cvpA"
                     /locus_tag="OO609_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6631970.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42168..42830)
                     /gene="dedD"
                     /locus_tag="OO609_000429"
     CDS             complement(42168..42830)
                     /gene="dedD"
                     /locus_tag="OO609_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6631971.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42820..44088)
                     /gene="folC"
                     /locus_tag="OO609_000430"
     CDS             complement(42820..44088)
                     /gene="folC"
                     /locus_tag="OO609_000430"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6631972.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44158..45072)
                     /gene="accD"
                     /locus_tag="OO609_000431"
     CDS             complement(44158..45072)
                     /gene="accD"
                     /locus_tag="OO609_000431"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6631973.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45228..45887)
                     /locus_tag="OO609_000432"
     CDS             complement(45228..45887)
                     /locus_tag="OO609_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6631974.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45970..46782)
                     /gene="truA"
                     /locus_tag="OO609_000433"
     CDS             complement(45970..46782)
                     /gene="truA"
                     /locus_tag="OO609_000433"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6631975.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46782..47795)
                     /locus_tag="OO609_000434"
     CDS             complement(46782..47795)
                     /locus_tag="OO609_000434"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6631976.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47861..48997)
                     /gene="pdxB"
                     /locus_tag="OO609_000435"
     CDS             complement(47861..48997)
                     /gene="pdxB"
                     /locus_tag="OO609_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6631977.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49096..50091
                     /gene="flk"
                     /locus_tag="OO609_000436"
     CDS             49096..50091
                     /gene="flk"
                     /locus_tag="OO609_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6631978.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50088..51266)
                     /locus_tag="OO609_000437"
     CDS             complement(50088..51266)
                     /locus_tag="OO609_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6631979.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51521..52762)
                     /gene="fabB"
                     /locus_tag="OO609_000438"
     CDS             complement(51521..52762)
                     /gene="fabB"
                     /locus_tag="OO609_000438"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6631980.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52921..54927
                     /gene="mnmC"
                     /locus_tag="OO609_000439"
     CDS             52921..54927
                     /gene="mnmC"
                     /locus_tag="OO609_000439"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6631981.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55048..55326)
                     /locus_tag="OO609_000440"
     CDS             complement(55048..55326)
                     /locus_tag="OO609_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6631982.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55360..55908)
                     /gene="epmC"
                     /locus_tag="OO609_000441"
     CDS             complement(55360..55908)
                     /gene="epmC"
                     /locus_tag="OO609_000441"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6631983.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55908..56717)
                     /locus_tag="OO609_000442"
     CDS             complement(55908..56717)
                     /locus_tag="OO609_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6631984.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56717..57541)
                     /gene="mepA"
                     /locus_tag="OO609_000443"
     CDS             complement(56717..57541)
                     /gene="mepA"
                     /locus_tag="OO609_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6631985.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57545..58630)
                     /gene="aroC"
                     /locus_tag="OO609_000444"
     CDS             complement(57545..58630)
                     /gene="aroC"
                     /locus_tag="OO609_000444"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6631986.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58665..59597)
                     /gene="prmB"
                     /locus_tag="OO609_000445"
     CDS             complement(58665..59597)
                     /gene="prmB"
                     /locus_tag="OO609_000445"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6631987.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59763..60314
                     /gene="smrB"
                     /locus_tag="OO609_000446"
     CDS             59763..60314
                     /gene="smrB"
                     /locus_tag="OO609_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6631988.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60418..61308)
                     /locus_tag="OO609_000447"
     CDS             complement(60418..61308)
                     /locus_tag="OO609_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6631989.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61295..61819)
                     /locus_tag="OO609_000448"
     CDS             complement(61295..61819)
                     /locus_tag="OO609_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6631990.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61816..62286)
                     /locus_tag="OO609_000449"
     CDS             complement(61816..62286)
                     /locus_tag="OO609_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6631991.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62283..62831)
                     /locus_tag="OO609_000450"
     CDS             complement(62283..62831)
                     /locus_tag="OO609_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6631992.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62836..63558)
                     /locus_tag="OO609_000451"
                     /pseudo
     CDS             complement(62836..63558)
                     /locus_tag="OO609_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63578..64300)
                     /locus_tag="OO609_000452"
     CDS             complement(63578..64300)
                     /locus_tag="OO609_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6631993.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64360..65514)
                     /gene="yfcU"
                     /locus_tag="OO609_000453"
                     /pseudo
     CDS             complement(64360..65514)
                     /gene="yfcU"
                     /locus_tag="OO609_000453"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(65463..66218)
                     /locus_tag="OO609_000454"
     CDS             complement(65463..66218)
                     /locus_tag="OO609_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6631994.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66300..>66847)
                     /locus_tag="OO609_000455"
     CDS             complement(66300..>66847)
                     /locus_tag="OO609_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6631995.1"
                     /translation="TAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVPDDH
                     KLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYT
                     IFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFNAWLVG
                     AADAPDLGNFEANTTFQITYL"
BASE COUNT        16633 a        18123 c        16655 g        15436 t
ORIGIN      
        1 ataaacgctc ataattcatt ataaataata atgaaatgac gccttttatt gataatttac
       61 atatgaaaaa attttccttt tgttatatta tttctgtttt cactccctct gattattttt
      121 accaaccctg ggtcactgca ttatcgccaa cgccccgaca cgtaagtcct gagcaattag
      181 aaaaaacagt tcgttatctt acacaaactg tgcatccacg taatgccggc aatattgata
      241 atctgaatag gtccgcagag tatataaaag agttctttgt cagtagcggt gccagagtta
      301 ccgcgcaggg cgtccccgat tggctattac gactctgcgc ctggaagcca gaattaccct
      361 tacccggcaa tgtcctggct ttatcccgat cggggagact ttattgctgt ggtcggcaga
      421 atgcaggata tcaacgccgt tcgtcaggta aaagcggcat tgttgtcatc ccgggattta
      481 tctgtttatt ctatgaatgc cccagggttt attcccggta ttgatttttc tgatcacctc
      541 aattattggc aacacgatat tcccgccgta atgattactg acaccgcttt ttatcgtaat
      601 aaacaatacc acttgcccgg tgataccgca gacagattga attatcagaa aatggctcag
      661 gtggtagatg gtgttataac cttgttatac aacagtaaat aataaaaatt tctcgaggcg
      721 ggtccagaaa cagcgacatg tacattttat tttccctcgt ctgttttaag atgcgaaaca
      781 taaaattcaa tggaggaaaa ataaatggga atgattggct attttgcaga aattgactca
      841 gaaaagatta accaactgct tgagtctact gaaaaacctt tgatggataa tatacatgac
      901 acgctttcag gattacgccg actggaatca ctggatcgaa gtgagctgcg taaacagttt
      961 tccattaagc gactcaatga aatggagatt tatccgggag tgaccttttc agaagagtta
     1021 gaggggcaac tctttgcctc gatcatgctt gatatggaaa agctgattag cgcgtatcga
     1081 cgtatgctgc gacaagggaa tcatgcgctg acggttattg ttgggtgatg gtgtcagagc
     1141 gccaaaaaaa accgccgaac atgtcggcgg ctttctgact tacaaactaa cagtttacat
     1201 cagcggcatt gctaaccgca caatactaat cagcggttgt ggccatacac ccagcaccag
     1261 taccaacagt gcagaaatca gcaccacgat accgcccgcg ctgtactgcc agtttgacgg
     1321 tgcatcgcga cccggttgtt ccggtgcgtg caggtacagg ctcaccgcca cgcgcaggta
     1381 gtagtagagg ccgattgccg aaccgacaac cacggcaccc accagccacc acaagtgtgc
     1441 ctggacaccg accgccagca cgtagaactt accgataaag cccagcgtca tcgggatacc
     1501 ggccagagac agcatcatca ccgtcatcac tgccgcgagg atcggacgat gccagaacag
     1561 accgcggtaa gagaacaggg aatcagcatc cgggccacga tacgggctgg acatcaggct
     1621 gaccacgccg aacgcgccga ggctgctgaa cagataaccg gccaggtaaa cccctaccgc
     1681 ttccatcgac atctcgccgg tttgcagcgc aatcagcgcc accagcagat agccgaggtg
     1741 agagatagat gagtaaccga gcagacgttt gatgttggtc tggctcaacg ccatcaggtt
     1801 accgaagatg atggaggcaa aggcgataat cgccagtact acgcgaatcg cttcgctgtc
     1861 acccaccggt gcgtagagga acagacgcat caccacaccg aagatagcga ttttgctcgc
     1921 cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg tatacgtctg gcgtccacag
     1981 gtggaacggc accagagaga gtttgaagcc gaggccaaca atcatcatgc cgaaacctgc
     2041 cagcaacagc ggctcgttga gcataccgtc gccgaggttt ttacccagcg cgacaaacga
     2101 caggtcgcca gactgcgcat acaccagcgc cataccgaac agcaggaaag aagacgctgc
     2161 ggcagaaagg atggtgtatt tgatactggc ttccagcgaa cgtttctggc ggaaagcgta
     2221 accgaccagg ccaaacagcg gcaaagagat cagttcaata ccgaggaaca gagacgccag
     2281 atggtttgca ttcgccagca ggatcccgcc cagcgcggca attaacacca acaggtagaa
     2341 ctcatccttg ttgtcgttat agccttcaag ccacgggtag gcgaaagtac aggtggcgag
     2401 gctcgccaac aataccagcc cggtgtaaag catggcgaaa ccatcaacgc gcatcagcgg
     2461 cgtaacgtcc atagcgcccg cctggccaac aaaccagagc gaaaccagcg ccgcgttaag
     2521 cccaataacc gagagcgtag cgttgaggaa atgattgcgt cgccacgcaa tggagagcat
     2581 cacaaccacc accgtcaagc cgacgatcag caacggtagc agtgcgatca ggttttgtgg
     2641 agttattgtc atggcgattt acggccttgt agtagtaacg gaattaacaa accactgctg
     2701 gatattgcca atcgcggagt gcgaggtatc cagaatcggc tgcggataga agcccagcag
     2761 taccagcagc accaccagca acaggatcat aaacagctca cgcagcgaca tccctggcag
     2821 ttcctggctg gcaatctggc ttttcgcttt accgaagtaa gcgcgatgca acatcgccag
     2881 cgaataaaca gatgcaaaga ccagcccaaa ggtagagata acggtaatca ccgggacaac
     2941 ctggaagctg ccgaacagaa tcataaattc gccgacgaag ttaccggtgc caggcatccc
     3001 aagcgttgcc accgcaaaga acagcgacag tgctggcagc catttcatct tgctccacag
     3061 accgcccatc atgcgcatgt cgcgggtatg gatacgttca taaagctgac cgcagagaat
     3121 aaacagaccc gccgccgaca agccgtgcgc aatcatctgg attaccgcgc cctggtaggc
     3181 caactggctg ccggtgtaga ttgcaatcag cacgaagccc atgtgggaaa ccgaggtgta
     3241 ggcgatcaga cgtttgatat cggtctgagc gaaggtcatc cacgcaccgt agaagatgcc
     3301 gataacaccc agccacatag cgattggcgc gaactctgcc gacgcgttcg ggaacagcgg
     3361 cagggagaaa cgcaacaaac cgtaagcggc ggttttcagc aagatccccg cgaggtccac
     3421 ggaaccggcg gtcggagcct gggagtgcgc atccggcagc cagccatgca gcggaaccac
     3481 cggcattttg actgcgaagg cgatgaagaa gcccagcatc aacagatatt ccacaccact
     3541 ggacattggc gtattcagca gctcttcata gttgaaggtc cagacgccgg tcgcattgta
     3601 gtgaacaaaa accagcgcca ggatggcgat caacatcacc agaccactcg cctgggtgta
     3661 aatgaagaac ttggttgccg ccgtgatacg cgttttaccg tcagaggctt tatgccccca
     3721 cagtgcgatc aggaagtaca tcggcaccag catcatttcc cagaagaaga agaacaggaa
     3781 catgtcgatg gcaaggaaca cgccgataac gccgcccagg atccacatca ggttgaggtg
     3841 gaagaagccc tgatattttt cgatctcttt ccacgaacat agtaccgcca gcacaccgag
     3901 cagaccggtc agcacgacca tcagcagcga cagaccgtca atggcgagat gaatagagat
     3961 accaaaacgc gggatccacg gcatgtcgaa ttcagactgc cactgcggaa ttccggcgga
     4021 ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc gacagcgcca gcgtcaatcc
     4081 catggtgatc agcgcgatcc agcgtggcac cttgacgcca aagcgttcgg tctgccagca
     4141 caggaagccg ccaataaagg gaattaatat tagccagggt agtaacatgg cgatctttat
     4201 tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat tctcacgaca gaaaattctc
     4261 ttcggactgg gggtagtgtc ggatgcgccg cgaaggcagc ttatccgacc tacgttctta
     4321 tatgcccagg ggcaatccca caatccttaa ctcaacgcag taccatcaac agtgccagca
     4381 ccacgaccgc accgatgctc atggatgcca cataccagcg cagataaccg ttctcgctta
     4441 acagcagacc tttacctgca aagcgggaaa gaaccgccgg gatgttcatc attgagttca
     4501 gcggatcgcg tttcagcaac caggcaatac ccaggaacgg cttgacgaac actttgtcgt
     4561 acagccagtc aaagccccaa gcgttgtacc accaggtact cagcaggcgg cccggcgcac
     4621 tgttggcgat ggaggtcacc agagaacgtt tacccagcca cagccaggct gccagcagaa
     4681 tgccgaccac cgcgaccacg ccagaggtaa tttccagggt caacatgctg ccgtgcgcca
     4741 gttccgtcgt ttgcggaagc acgccctgca gcggcggtac aatcagtgcg ccaacgaagg
     4801 tggaaaggat cagcagcaca atcagcggca ggctgtgagt tacccctttc acggcgtgag
     4861 cgtgaatttg ttcttttccg tggaagacga tgaaaatcat acggaaggtg tagagcgagg
     4921 tcataaacgc accgaccaga cctgccacca tcagattgat atgaccattc gccatcgcac
     4981 ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac cagcggcagt gccgacagtg
     5041 ctgcgccgcc caccaggaag cagagataaa ccagcggaat agatttacgc agaccgccca
     5101 tcttgaagat gttctgttcg tgatggcagg ccagaatgac ggaaccggat gccaggaaca
     5161 gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat cgccgcatcc catgcctgca
     5221 cgccaagcgc gaggaacatg tagccaatct ggctcatggt agagtaagcg agaacacgtt
     5281 tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag cagcgtaacc gccccgacaa
     5341 tacccaccag atgcagaact tccggcgtca tcaggaacag gccgtgggta cgggcgatca
     5401 ggtagacacc cgcggttacc atcgttgcgg cgtggatcag cgcggagaca ggcgtcgggc
     5461 ccgccatcgc gtcagcaagc catgtctgca acggcaactg cgcagattta ccgaccgcac
     5521 cgcccagcag catcagcgtc gcccacatca gcatgttatt gccgtcagca aagtgcgctg
     5581 gtgccagttc caccatatcg cggaagttca gggtgcccag ttcgttgtaa agaatgaaca
     5641 gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac gaacgctttc attgctgctg
     5701 cgccattctt cggatcggtg taatagaaac cgatcagcag ataggagcac aggcccacgc
     5761 cttcccagcc gaggtacatc agcagcaggt tatcggcgag aaccagaact accatgctgg
     5821 cgatgaacag gttggtgtag gcgaagaagc gagagtagcc ctcttcaccg cgcatgtacc
     5881 aggaggcgta catgtgaata aggaaaccca caccagtgac caccgagagc atggtcagcg
     5941 acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc gcctaccgac atccacgtcc
     6001 acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa atcaacgccg ataaaggcgg
     6061 ttaccagcgc cgccaggccc acagagccaa cgccgacgat cgccgaaacg ttttcagacc
     6121 agcgcccacg ggagaacgcc agcaggacga agccaatcaa tggcaaaata atggttaagg
     6181 caagcatgtt catccgcgca tctcacttac tgaatcgatg ttcaggttct ggcgacgacg
     6241 gtgaagttgc agcagcagcg caaggccgat actcgcttct gccgccgcga ggctgatggc
     6301 gagaatgtac atcacctgac cgtcggtctg accccagtag cttccggcga ccacaaaggc
     6361 cagcgcggag gcgttaatca tgatttccag accaatcagc ataaacagca gattgcgacg
     6421 gataaccaga ccggttaagc caagaacgaa taagattgcc gcgaggatca gtccatgttg
     6481 taaggggatc atgcgtgctc ctccgttttt cttttcgcgc tgtcgtcttt acgattgctc
     6541 agcacttcac ccgcacgctc ttcacgaccg acgtggaagg ccacaaccag acccgcgagc
     6601 agcagcatag aagccagttc caccgccagt acgtaaggcc cgaacagcgt aatacccact
     6661 gctttagcac tgattggcgt accgtcgata ccctgatcgt taacaccgag gatggcgtaa
     6721 acaatcacca ccagcatgat ggccgacaaa attgccgggc caatccacac ctgcggtttc
     6781 agccactggc gttcctgttc gatttctgaa ccgcccaggt tgagcatcat caccacgaac
     6841 acgaacagca ccataatggc acccgcgtag acgataattt ccagcgcacc ggcgaagtaa
     6901 gcgcctagtg agaagaacac cccggaaatc gccagcagcg aaataatcag gtacagcagt
     6961 gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg caagtatggc tatcaggcca
     7021 cagatataaa aagcgaactc cattgcccct ctccttacgg taacaggctc ttgacgtcga
     7081 taggcttggc ttcgttctct gcttcgccct tatctttgcc gtcgattgcc atacctgcca
     7141 tccggtagaa gttatattcc gggtatttgc ccggaccgga gatcagcaga tcctctttct
     7201 cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa atccggggtt aactgaatcg
     7261 ccgtggtcgg acaggcttct tcgcacagac cacagaaaat gcagcgtgag aagttaatgc
     7321 ggaaaaattc cggataccag cgaccgtctt tggtttctgc tttttgcagc gagatacagc
     7381 cgaccgggca ggctaccgca cagaggttac aggctacgca acgctcttcg ccgtccgggt
     7441 cgcgggtcag aacgatacga ccacgataac ggggcggcag atagaccggc tcttccgggt
     7501 acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat catccagata ctacgaacct
     7561 gggtgccgaa acctactaac aattctttta aggtcatggt cttattgccc cttattgcgc
     7621 ctgccagaga atgacagccg ccgttaccag caagttgatc agcgtcagcg gcaggcagat
     7681 tttccagccg aaggacatta cccggtcata acgcggacgc ggtaacgacg cacgaatcaa
     7741 aatgaacatc atcataaaga acgcggtttt cagcgcgaac cagatgaatg gcggtaacaa
     7801 cgggccttgc cagccaccga agaacagcgt caccatcaat gcagagatgg tcacaatccc
     7861 gatgtactca cccacgaaga acagaccgaa cttcataccg gaatattcaa tgtggtaacc
     7921 atccgccagt tcctgctcgg cttccggctg gtcaaacggg tgacggtgac acaccgccac
     7981 gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg atgacgttcc acacatgcgc
     8041 ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc tgcgccacca cgcccatcag
     8101 ggaaagcccg aggaacactt cgtagctcag ggtctgcgca gaagcacgca tcgcacccag
     8161 cagcgagtat ttgttgttac ttgaccagcc cgcaaacagc accgcgtaaa ccgccagacc
     8221 tgccatcatc aggaagaaca aaatcccgat gttcaggtcg gcaaccaccc aacccggact
     8281 gactggcaca attgcaaagg ccagcagcag cgaggtaaag gcaatcatcg gtgccagggt
     8341 aaagatgacg cgatccgaga atttcgggat ccagtcttct ttaaagaaca ttttgatcat
     8401 gtccgcaacc agctggagcg aaccgcccca gccaacacgg ttaggtccat aacggttctg
     8461 gaacagaccc agcaggcgac gttcgccaaa gctcatgaat gccccacagg tgacaaccac
     8521 cagcaggatc accaccgctt tgaggatggt cagcaggatc tcaatcagtt ccggtgatat
     8581 ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg cgccagccag caccggagca
     8641 atgccggaca tacccatcgg caagcccacc tgccctgccg tcagtccttc ggcgatttca
     8701 accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga cgcgtgtacc tgcgttcaca
     8761 cccaacttcg cggcatccgc tgggttgagt ttgatgtacg gctgcggcat acggctctgg
     8821 aagaccggag cacgctgtga caattcatcg ctgccaaaca ggtggtagta cggcgcgata
     8881 cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc tggtgaagta atccagaccg
     8941 ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa agcgcagttt gccgcccact
     9001 tcgtcctgga atttgttcca cgcctgcggg gagttccagc ccggcgccca ggcaaacggc
     9061 acttgcgaac ggtgcgcggt cggctggttg ttaccttcca tcgagaaggt gaacatggtg
     9121 tcaatatcct gcggctgacg cggctcatga acgctgatat tggcgcgcat ggcggtacga
     9181 ccgctgtaac ggtgcggttc acgggccagt ttctgcccac gaatacggaa tgtcgcatcc
     9241 ggcgcagcat ctttgatacc cgccagttct gggattttcg ccacaacagc gtcaattaca
     9301 tggtcgagct gcgtccagtc cacttcacgg ctcagcaagg tgctgtgcag cgagtgcagc
     9361 cagcgccagc tttccagcat gacagttttg ctgtcgtaat aagccggatc gtaaacctgg
     9421 aagaaacgtt gggcgcgacc ttcattatta attaccgtac cgtcgctttc agcaaagctg
     9481 gcagaggaaa gcaccagatg ggcgttttcc atgatcgctg tgcgttgatg atcaaccacc
     9541 atcaccagcg gcgctttagc cagcgcagca ttcacgcggg tagcagaagc gtaacgatgc
     9601 agatcgtttt ccaacaccac caccgcgtcg gcgcgtccgg tttccagttc ggttaacgct
     9661 tcttcaagcg aaccgccacc cataatgccc agccccatgc tgttgacgga acgggcaatc
     9721 atggtgatac caacgtcagc gccgcgacct ttcagggctt tcgcgacgtt agccgcagcc
     9781 tgaatcacct ctatgctacc ggcgttcgtc ccggagataa tcaacggttt cttcgcacct
     9841 gccagtgcct gcacgatgac gtcgattttg ttttgcagct caggttcgat accgtcaacc
     9901 gctggtgcgg agttatccag cgcatgggcg atggcaaaac ctaaacgcgc ctgatcttca
     9961 accggtgcgc ggtaagtcca cgccgcgata tcatccagac gagtgtcatc aacgttggta
    10021 acaaacagcg gatgcttcgc acgttgaccg atgttgagga ttgccgcaat ctgccagtca
    10081 gccactttct gtgctgcagc catttcgcgc gctttacctt tcaccgcctg acgcactgcc
    10141 agtgcgacgc gcgcgccggt ctgggtaacg tcttcgccca gcaccagtac cgcatcgtaa
    10201 gattcgattt cgcgcagagc cggagtataa atgccgcctt cacgcagcac tttcagcgcc
    10261 agttgcagac gttcctgctc accgtgagcg ataccggtgt agaagttttc ttcgcccacc
    10321 agttcacgca gcgcaaagtt gctttccacg ctggcacgcg gagaaccaat accgatcact
    10381 ttcttcgact gacgcagaat atctgccgcg ccctgcattg cctgttcggc gttgagggta
    10441 atgaaatcat cgccacgacg ctgtactggc tgacgcggac gatccttcag gttgacgtaa
    10501 ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt ttaccgtacc gttgtaacgg
    10561 ttttcgatac gacgcagttc gccgtaacgt tcaccggggc tgatgttaca gccgatggaa
    10621 cattgctggc agatgctcgg cgcaaactgc atatcccatt tacggttgta acgctcggag
    10681 tgcgttttgt cggtaaatac gccggtcggg caaatttcga ccaggttacc ggaaaattcg
    10741 ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt tgtcgtgcgc gccataaacg
    10801 cccagatctg taccgtcagc gtaatctttg tagtaacgca cacagcggta gcaggcgatg
    10861 cagcggttca tttcgtgaga gatgaatggc cccaaatcct gattacggtg ggtacgtttg
    10921 gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg tcatatcctg aagatggcag
    10981 ttaccgcctt cttcacatac cggacagtcg tgcgggtggt tggtcattaa ccactcgacc
    11041 acactttcac ggaactgttt cgcttcttcg tcgtcaatgg aaataaaggt gccatcggaa
    11101 gccggtgtca tacaggacat caccaggcga ccacgcgtgt cttccgcgtt ttggtattgc
    11161 ttcaccgcac actggcggca agcaccgacg cttcccagcg ccggatgcca gcaaaagtaa
    11221 ggaatatcaa ggcccagaga cagacaagct tccagcaggt tgtccgctcc gttgacctcg
    11281 tattctttgc cgtctacatg aattgtagcc attagcatgc ttccagtttt ctcggtcagg
    11341 gaccgagcgt taatcgaaat tcggttacca gcgctctttc agcaggttcg gctgaatccc
    11401 attaatcaaa tgggtattgc tgaacggctg tttgattccc gcctcaaatt cttcgcggaa
    11461 atatttgatg gcgctctgta acggctccac tgcaccaggt gcgtgggcac agaaagtttt
    11521 acccgggcct aagaatcgac acagttgctc aagtgtttcg atatcgcccg gctggccttc
    11581 gccacgctcc agcgcacgca gaattttcac gctccacggc agaccgtcgc ggcacggcgt
    11641 acaccagccg caggactcac gggcgaaaaa ctcttccagg ttacgcacca gcgacaccat
    11701 gttgatctca tggtcaaccg ccatcgccag cgccgtaccc agacggctgc ccgctttacc
    11761 gatactttcg aattccatcg gcagatcaag gtgcgcttcg gtcaggaagt cagtccccgc
    11821 gccgcctggc tgccaggctt taaatttcag accatcacgc ataccaccgg cgtaatcttc
    11881 gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac agtcccggat ttttcacccg
    11941 accggagaag cccatcagct tggtgccagc atctttactt ttcgagatgt tctgatacca
    12001 ctccacgccg ttagcgagga tcgccggaac gttacacagg gtttcgacgt tgttgacaca
    12061 ggtcggtttg ccccatacgc cggaggttgc cgggaagggg ggtttcgagc gtgggttagc
    12121 acgacgccct tccagggagt tgattaacgc tgtttcttcc ccgcagatgt agcgccctgc
    12181 cccggtatgg acgaacagtt cgaagtcgaa acctgttccc ataatgtttt tgccaagcaa
    12241 gcccgcttca gtggcttcgg caatggcgcg gcgcagattt actgccgctt cgatatattc
    12301 gccacgcagg aagatgtagc cacggtaagc tttcagcgca aacgcggaga tgagcatacc
    12361 ttccaccagc aggtgcggca gttgctccat caacaggcgg tctttatagg tgcccggctc
    12421 catttcatcg gcgttacaca gcaggtaacg gatgttcatg gattcgtctt tcggcatcag
    12481 gctccacttc aggccagtcg aaaagcccgc gccgccgcgc cctttcagac cagcgtcttt
    12541 tacctgatta acgatttcgt ccggagacag cccggtcagc gccttacgcg ccccttcgta
    12601 accgtttttg ctgcggtatt cgtccagcca cactggctgt ttgtcatcgc gcagacgcca
    12661 ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc atttataccg ctccagcagt
    12721 tcagggatcg cttccggggt cagatgcgcg tgagtgtcct catcgatcat catgtttggc
    12781 cctttatcac agttccccag gcagcaagtt ggcagcagcg taaagcggcc atcaaatgtc
    12841 gtttgccctg gtttgatgtt cagctttttc tcgagcgccg cctgaatacc ctgatacccg
    12901 ttgatatgac agaccacgct gtcgcaataa cggatcacat ggcgaccaac cggctggcgg
    12961 aagatctgac tgtagaacgt tgccacacct tcgacgtcgc ttgccggaat acccagcaca
    13021 tcggcgatcg cgtggatcgc accatccggc acccagccac gctgcttctg aacgattttc
    13081 agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt gcatctcgtg ctcaatcgct
    13141 tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg gttgttgatt ctcgtgcata
    13201 attagcggtc cacatctgac ataacaaaat cgatactgcc cagataaaca atcaggtcag
    13261 acaccaggct gccgcggatc gccgccggaa tttgctgcaa atgcgcataa ctcggcgtgc
    13321 ggatacgagt gcgataactc atggtgctac cgtcgctggt caggtagtaa ctgttgatcc
    13381 ccttggtcgc ctcaatcatc tggaaagatt cattggcagg catcaccggc ccccacgaca
    13441 cttgcaggaa gtgggtgatc agggtttcga tatgttgcag cgtgcgctct ttcggcggcg
    13501 gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg catgttgttg aggcactgct
    13561 caagaatgcg cagactctgg cgcagctctt ccactttcag cattacgcgg gtgtagcagt
    13621 cagaaacacc accaccaacc gggatttcaa agtcgaagtt ttcatagcca gaataaggac
    13681 gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag gcccgcgcca gtggtgcccc
    13741 actccagcgc ctctttcgcg ccataggcgg caacgccctg ggaacgacct ttcagaatgg
    13801 tattttgcag cgccgctttc tcgtaagacg ccagacgttt cggcatccag tcgaggaact
    13861 cacgcagcag acgatcccag ccgcgcggca ggtcgtgcgc tacgccgcca atacggaacc
    13921 acgccgggtg catacggaaa cccgtgatcg cttccaccag atcgtaaatt ttctgacgat
    13981 cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc ctgaataaag gtcgagatgt
    14041 acagcaggtg gctgttgata cggaacagtt cggagagcat aacgcgaatg acgttaacgc
    14101 gatccggcac ggtgatcccg gccagtttct ctaccgccag cacgtaaggc atttcgttaa
    14161 cgcagccgcc gaggtattca atacggtcgg tatacggaat gtagctgtgc caggactggc
    14221 gctcgcccat tttctccgca ccacggtggt ggtagccgat gtctggtacg cagtcgacaa
    14281 tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc gtgcgccgac gggtggttcg
    14341 gaccgaggtt gaggaacatg aagtcctcat tttcggtgcc gcgcttcatc ccccactctt
    14401 ccggtttgaa ggtcagggct tccatctcca gatcctgttt ggctttggtc agctcaaacg
    14461 gcgagaattc ggtagcgcgt gccgggtaat ctttacgcag cgggtgacct ttccaggttt
    14521 gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa agtaatgcca aacagatccc
    14581 aggtttcacg ctcataccag ttagcgttcg ggaacagttt ggtgaaggtc ggtacgtgca
    14641 ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg gttacgatcg atagaaatca
    14701 gatggtagaa aacggaaaaa tccgcggcag gtaacccttc gcggtgtgtg cgcagacgtt
    14761 cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt cggcagtttc tttaagaaat
    14821 cgccaacttc cagtaattgt tcacgcttga tccacacaac gggaaccccg gtgcgagtcg
    14881 cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag ttcgccaatt accggatcat
    14941 caagatgatc gcgggtctgc caggcgggtt cttgcgcggt taagtcggtc atattgttca
    15001 ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc ttcgcgaaat agatttagga
    15061 ataagcgaag aaaaatccct ttgccgacag gcgcaaatta aatctcgtca ggtgtacgca
    15121 ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg ctctgattgc atattggcgc
    15181 gataaacgcc ctgatcgcca accacccagg agagcggacg acgttctttg ccgatagatt
    15241 cctgcaacag catcagtgcc tgcatgtacg cttcaggacg cggcgggcag cccgggatat
    15301 acacatcaac cgggatgaat ttatcgacgc cctgcacaac ggaataaata tcgtacatac
    15361 caccagagtt ggcacaggca cccattgaga taacccattt tggttccagc atctggtcat
    15421 acagacgctg aataaccggt gccattttgg taaagcaggt tcctgcaacc accatcaggt
    15481 cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa acgcgccacg tcatgcaccg
    15541 cggtaaacga agtcaccatc tcaacgtaac agcaggaaag accgaagtta tacggccaaa
    15601 ttgagttttt acgaccccag ttaaccatgt cattgagctt gcccataaac acgtttttgt
    15661 taacttcttg ctccagaggg tcggttacga tctcctgctt ttgcaggggg taacggtcgt
    15721 tctcaccgtt gggatctatg cgggtgagcg tataatccat cttaatgcct cgcggttagc
    15781 gttgacgatt agcgatactg ttcgtttccg ggttcatacg ctcgcggcgt gaacgcgcgg
    15841 gcgtccagtc cagcgcgcca atacgcacca gataaaccag acctgccagt aacacaaaaa
    15901 taaaaattgc agcttccaca aagcctaccc agccgctttc gcggatagag gttgaccatg
    15961 cgaacagata cagcgcttca acgtcgaaga taacgaagaa catggccacc agataaaact
    16021 tggcggacag gcgtaagcgg gcggagccga ccgagtcgat accggattca aacggcacgt
    16081 ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc gcctaccagc atcaggcaac
    16141 acaggccaat ggcaacgata agaaagatag cgaatgccca gtgatgagcg atgacttcag
    16201 tggatgttga catactcatt gcttactcat caaaagtagc gccagattca ctgctcttca
    16261 cggcagatgg acgccacatc gattcatggg gaggaataaa aaaaacctta caataactgt
    16321 agaaattctt ttatacagct aattgatgtg gtcttttact cctttctata accttttgtc
    16381 aactttaaca aaagcttctt cacattagtt tacataatat caacaccatt agcatttaat
    16441 gccctttcac cccagatcct tgacgactcc aggataatta gatgttgttg aatcgtgtcc
    16501 gttgtgaagc aatggaaaaa atacgggtct attttgacag gaatttgtgt cgattcctcc
    16561 ccccaaaaga gagtattttc ttgatctgtg acacgctttt gttattccat aacaaaaacg
    16621 cagcaacaaa tttacgtatt ttttaacatc attgtagcag gtgatttttt tcaggcgatt
    16681 atttgtgcat tcgggacgtg aatctctggt gattgaaaaa taaacagttt tgtacgttct
    16741 gcactatgcg aggaaggatt tttactaaaa aaaagccgct ggggtttaaa acacccccag
    16801 cggctcgttt tttacactat tgtctcagga attatctatc gtccgtcgat tactcgatat
    16861 ccctttcaat caacaatgaa tcatcccctt ccggagcaga cattgggctg tattgccacg
    16921 gattatggta gctctccatc gcctgataga tcacctgggc cagctcatta ttactggacg
    16981 ggtcatagca cagcaaatat tcggtatcag gtagcggcgg taagccatct actccactca
    17041 gcacacgcag gtccgggctc atcatctcaa ccggccttgc cgtcacgcca agaccggctt
    17101 tcactgccgc acgaacggcc ggaagcgtcg aggcgacata agccagtcgc catggaatat
    17161 ctactttatt aagcgtcgcc agcaccatat cgcgaaacgg acttggatca tccagcaata
    17221 caagagggat cggctcgcct ttttgcaata cgtattccgc tgcgcagtac cagtgtgttg
    17281 gcgaggtacg caggttcaat gccttaaacg ctgaaggacg atgggtggtt accatcaaat
    17341 ccacttcctg ggattccagc atttctgcca tataggcatt acgtttaacg cggacatcca
    17401 gcgctaattt cggataaacc gaactcacgc gatttaacag gaaaggtaag atcgtatcgg
    17461 cagattcatc tgaagcaccg atagttaaca cgccctgaag attactgaac attaatgatg
    17521 agcaggcctc atcattaaaa cgcaggattt tcctggcgta accaagaagt tgaatgccat
    17581 gttcagttaa cagtttgttg cgaccgtgac gagcgaacag ttctttccca acgagttgtt
    17641 ccagacgctg catttgctga cttacggcgg actgagtacg acacacagcg gcagctgcgg
    17701 cagcaaaagt gttcagatcg gcaacagcaa caaatgttct cagcagatcg aggtcgaggt
    17761 taattatcgg acgatttgca cttatcatat attatcactt actggcggct catactgagc
    17821 tggttaatgc tgtgcacaca caaacaagca attccatttg taatgtgcct ccctggcagt
    17881 ttcatccgga acccggacga aagtaaaaat gcatatgagt tgcactaaaa aagcgactca
    17941 cattgttccg ttataatgcc tgaagtagat cacagaatat atcttcaggg atcgcatatc
    18001 tattaagtta ctcactcttt tctatttatg acatgcgcgt gtttgtataa atgtaaatgt
    18061 gagtccttgt tccactctcg tgcagcatcg ctggtcatac gcgaacacgt acaaacagca
    18121 atggtgaagc ggcatcaaga gcagggatcc gtcatttatg cgagcatttt accccaaaaa
    18181 ccttttattt ataagggtca ttgcgaatta tctgatgcaa agttatgtta tgttaggcaa
    18241 agtaatcttc tattattaat aagcacatca aaaccttttt gaatattaaa taataattaa
    18301 ttagcatcat cctcattcat taattccgct taacaatagt ttcacacctt acccccattg
    18361 cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc
    18421 gtttcattag tgaattctta aatgaggcat tttcacacaa ttatcttaca gataaaaaaa
    18481 ccagacttgc aattaagaat tagaacaatt cactatataa cattgcatgt aaagcatata
    18541 agcctcatta tttgtcatta ttaagttatt aacagcacaa tcgagccttc ccctctggca
    18601 aaatcttatt ctgcagacct tcaaaacacc gtcctggggg agtacattgt tctaagctga
    18661 cttccacggc agggagtggc gataacagca aaaaaggtca agattcatgt cccccattga
    18721 aaaatccagc aaattagaga atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc
    18781 aaaacgcctg gaagaagaag gtaacaaggt actgaaactg aacatcggca acccagcccc
    18841 attcggtttt gacgcgccag atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc
    18901 tcaagggtat tgcgattcca aagggcttta ctccgcgcgt aaagccatca tgcagcacta
    18961 ccaggctcgt ggcatgcgtg atgttaccgt ggaagatatt tacatcggca atggtgtatc
    19021 ggagcttatc gttcaggcaa tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc
    19081 tgcaccagat tacccactct ggaccgcggc ggtttcgctt tccagcggta aagcggtgca
    19141 ttatctttgc gatgaatcct ctgactggtt cccggacctc gatgatattc gcgctaaaat
    19201 tacgcctcgt acgcgtggga ttgttattat caacccaaat aacccaaccg gcgcggtata
    19261 ttccaaagag cttttaatgg agattgtgga gattgcacgt cagcataatc tcattatctt
    19321 cgccgatgaa atttatgaca aaattctcta cgacgatgct gagcatcact caattgcgcc
    19381 gctggcacct gacctgctga ccattacctt taacggactg tcgaaaacgt accgcgttgc
    19441 aggcttccgt caggggtgga tggtgttgaa cgggccgaaa aaacacgcca aaggctacat
    19501 cgaaggtctg gaaatgctgg cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc
    19561 cattcagacc gcgctgggtg gttatcagag catcagtgaa tttattaccc ctggcggtcg
    19621 tctttatgag cagcgtaacc gcgcgtggga actgatcaac gatattccgg gcgtttcctg
    19681 cgtgaaacct cgtggtgcgc tgtatatgtt cccgaaaatc gacgccaaac gctttaacat
    19741 tcatgacgat cagaaaatgg tgctggattt cctgttgcag gaaaaagttc tgttggtgca
    19801 agggacggca ttcaactggc cgtggccgga tcacttccgc attgtcacgc taccgcgtgt
    19861 cgatgatatc gagctgtctt tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct
    19921 gtaatcttaa tttcactgcc ggagattgca tccggcagtg ttatcccgcc acaatgacct
    19981 gatgatgtca tcatacgtaa ggtcactatg aaacagagcc atttttttgc ccatctctcc
    20041 cgcctgaaac tcattaaccg ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc
    20101 gaacacagtt tgcaggtagc gatggtcgcc catgcgctgg cagctatcaa aaatcggaaa
    20161 tttggcggta atgtcaacgc cgaacgtatc gctttactgg cgatgtacca cgatgcctca
    20221 gaagtgctca ccggcgatct ccctactccg gtgaaatact tcaatgcgca aatcgctcag
    20281 gagtacaagg ctattgaaaa aatcgctcag cagaaactgg tcgatatggt tccggaagaa
    20341 ctgcgggata tctttgcgcc gttaattgac gagcatgcat atagcgatga agaaaaatcg
    20401 ctggtgaaac aggcagatgc actgtgtgca tatctgaaat gtctggaaga actcgcggcc
    20461 ggaaataatg aattcttgct ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc
    20521 agccaggaga tggactactt catggaagta tttgttccca gcttccatct ttcgctcgat
    20581 gagattagcc aggattcacc gctgtaagca gccggagtct gcgtcgcatc agtcaataag
    20641 cgccggatgc gacatcaggc tcttgtcaaa acggaaacag catcgggatc atcaccacac
    20701 aaaccgccat cacgataatg gtgaacggta cccccaactt cacaaagtca ctgaagctgt
    20761 aatttcccgg acctaaaacc agggtgttaa caggtgaaga caccggtgtc ataaaggcgg
    20821 cggatgctgc catcgccacg accatcgcaa atggataagg cgacactccc atcgttttgg
    20881 cagcagccag cgcaatcgga gccatcaaca ccgccgtcgc ggtattagag ataaacagcc
    20941 caataaccgc cgacaagaca aacaaacacc ccagcatcat atgtggccca taaccgccgc
    21001 caatatccat cagccctttc accgccagcg cgacacctcc cgttttctgt aatgccacag
    21061 caaacggcat catcccaacg atcaaaataa tgctcggcca gtgaatggat ttataggcgc
    21121 tttcagcatc tatacagcgg aatttcccca tcagcaggca ggcgatgata gcggcaacag
    21181 gattaggaat ttcatttgtc agcattaacg ccaccattag caccagacag aaaatggcat
    21241 ggggtgcctg gctgtgcgcg ggcgatgctt cactcacctc ttccggtaag ttcagcgcta
    21301 cgaagtcgcg gccctgtttg gccagcatac cgatcagttt ccagttaccc acaaccagga
    21361 tgatatcgcc cagcagcaga ggctcatccg ccagcgaacc ttccagcgcc acgccattgc
    21421 gcttgagccc caccacattc agtccgtagc gggtacgaaa accaatttcg cgcaccgatt
    21481 taccaatcag ttctgactca ggaattaatg aaatctctgc catgcccaca tcaagggcct
    21541 ggtcagaaaa atactcgccg cgcagtacca tcggctccag caattgctca ctacaaaatt
    21601 gccggagatc gacatcagcc gcagacatat caataagcaa aacgtcacgc gcgcgaaatt
    21661 cagaaacccc attaacgttc acgataacgc gacgaaaacg ccgccagcgt tcaacaccga
    21721 tgacgttagc gccataacgc tcacgtaatt tgagatcatc cagccgttga cctatcattg
    21781 gcgatccggg gcgaatagcc agacgtcgcg cacgcccggt cagtcgatat tcacggataa
    21841 gatcgcgaaa ggttcgacgc gtccagcctt cgcgctgcgg ggtctgggta tcccctttca
    21901 gcatgaaacg catcactaac atatacaaaa tacccagcac cagcacgacc aggccaatag
    21961 gtgttacgct aaagaaactg aagccgtgat agccttcacg cagcaattca ctgttgacta
    22021 ccaggttcgg cggcgtcgcc accagcgtca tcatgccgct aatcagcccg gcaaaactta
    22081 acggcatcat cagacgtgac ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg
    22141 gaataaagat agcgacaacg ctggttgaac tcataaacgc gccaagcccc gcgacggtca
    22201 gcatcaacaa aaccaacatt ttgatttcac tgttgcccgc cactttgacc agccatgttc
    22261 ccattacggt ggcaacaccg gtacggacca aaccatcgcc aataataaac aaggcggcaa
    22321 tcaggacaac gttaggatca gaaaagccgg aaaatacttc ggggaccgtc agcgttccgc
    22381 ttaatgcaaa cgcgacaata acaaacaaag cgaccgcatc catacgcact ctgcccgtcg
    22441 caaacaagac gatggcaacg gccagtaatg aaagaaccca aatcaattca ccgttcacaa
    22501 cttatccttg ttaattgagg gggatgactt gattctgcca taaaaaagcc ccgacgagac
    22561 ggggctaaat catgatcagg tgtttcactg aataataaca tcgccatttg gctgtttggt
    22621 cacagtaatt tgctccagac tgtggaggac caaatcgacc tcattcaggc gcggggtatc
    22681 tgccggagcg ttaaccgcaa tgacatggca acccgccgcc aggccagaaa gcacgccagc
    22741 gggagcatct tccaccacca cacactcctg cggcgcaagc cccagcagct gcgcacctaa
    22801 cagatacgca tcaggttctg gttttccgcg cttcactcgc tcagcggtta caaacacctc
    22861 tggtgcggga agccccgcta ttttatggcg cgctcgcgct accggcatgg agccagaagt
    22921 cacaatggcc cacggaatac ctgctttatt caaatgattg agtaaggcga ttgccccagg
    22981 aagcgcggta ataccttcgg tttccgtggc ctcgatttgc tccagacgcg taaactcggc
    23041 ggcaatatca gcctcggatt tgcccgccat aaaatggcgc aaagaggtga tcgcctgttt
    23101 accgtgaatg aaagccagca cctcttccgg cgctaaccca tgacgtctgg cccagttgct
    23161 ccacgcccgt tctaccgcag gcagggaatc caccagcgtt ccatcaagat caaacagaaa
    23221 acctttgcac cgcacgcggg cctcctcagg cgttgataat ttgattaatt tcgttggcgc
    23281 tcaaatggta ctgacgcggg caggcatgcc acacattaag catgcgctga tatttttccc
    23341 acattggcgt ctgggcgtta aaaccatgag tcccagcgtc aaaatgggta tagcgccctt
    23401 ccacattaac cataaagcgg acataaccga ggtaacgtgc ttcagtggcg gcgtcaaagc
    23461 cgaggaaggt gacacgacgt tcatcgatgg attgctggtc ctgcaaatta gaccaagaaa
    23521 catacaacgc atgatacata tccataatgt cgatgatagt gcggcaggtt tcttctttca
    23581 gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc gtaaccacgc tcaataattg
    23641 tttgcaggcg acggtaacgt tcagcatttg ccggatcgag catagtcatc atcttgtact
    23701 gattagacaa aataagacgt tgcgcgttgg tcatttccat tgttgactcc tgtatcactc
    23761 tactacggtg aaaaaaaaga aggctgagta tgccttcttt tatatgcgta atcaggggtc
    23821 aattacaaat catcaaggaa agttttatcc agttgtttga aggcgcgctt aagcgtgtca
    23881 gctaatgcct ggtaatcagg cttgccttca acgggtgcca acacctgtcc agactcctgc
    23941 aatttaccgc gaacttcata aaaccagtta aggattgcag ggggtaatgg cgttacagaa
    24001 cgcttgccca gccaccacaa tccctgcatg ggtaaactta aggcgaacag tgcagtggca
    24061 actgccggcc caagcagacc gcccagggca atctgccagc agagagtaaa tacggcgatc
    24121 ggcggcataa aacggatcgc ataacgcgtc atcttgataa cgcgattttc gacaaagacc
    24181 ggagcaaggc gtttttccag tggccacgtc tttgagtaat gctgtccgcg gcgaaacaag
    24241 ctaaaaaaat taacagaacg attatccggc gttgacatgc ttcacctcaa cttcacatat
    24301 aaagattcaa aaatttgtgc aaattcacaa ctcagcggga caacgttcaa aacattttgt
    24361 cttccatacc cactatcagg tatcctttag cagcctgaag gcctaagtag tacatattca
    24421 ttgagtcgtc aaattcatat acattatgcc attggctgaa aattacgcaa aatggcatag
    24481 actcaagata tttcttccat catgcaaaaa aaatttgcag tgcatgatgt taatcataaa
    24541 tgtcggtgtc atcatgcgct acgctctatg gctccctgac gtttttttag ccacgtatca
    24601 attataggta cttccatgtc gagtaagtta gtactggttc tgaactgcgg tagttcttca
    24661 ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt acctttctgg tttagccgaa
    24721 tgtttccacc tgcccgaagc acgtatcaaa tggaaaatgg acggcaataa acaggaagcg
    24781 gctttaggtg caggcgccgc tcacagcgaa gcgctcaact ttatcgttaa tactattctg
    24841 gcacaaaaac cagaactgtc tgcgcagctg actgctatcg gtcaccgtat cgtacacggc
    24901 ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg ttattcaggg tatcaaagat
    24961 gcagcttctt ttgcaccgct gcacaacccg gctcacctga tcggtatcga agaagctctg
    25021 aaatctttcc cacagctgaa agacaaaaac gttgctgtat ttgacaccgc gttccaccag
    25081 actatgccgg aagagtctta cctctacgcc ctgccgtaca acctgtacaa agagcacggc
    25141 atccgtcgtt acggcgcgca cggcaccagc cacttctatg taacccagga agcggcaaaa
    25201 atgctgaaca aaccggtaga agaactgaac atcatcacct gccacctggg caacggtggt
    25261 tccgtttctg ctatccgcaa cggtaaatgc gttgacacct ctatgggtct gaccccgctg
    25321 gaaggtctgg tcatgggtac ccgttctggt gatatcgatc cggcgatcat cttccacctg
    25381 cacgacaccc tgggcatgag cgttgacgca atcaacaaac tgctgaccaa agagtctggc
    25441 ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg ttgaagacaa ctacgcgacg
    25501 aaagaagacg cgaagcgcgc aatggacgtt tactgccacc gcctggcgaa atacatcggt
    25561 gcctacactg cgctgatgga tggtcgtctg gacgctgttg cattcactgg tggtatcggt
    25621 gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac tgggcgtgct gggctttgaa
    25681 gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat ctggtttcat caacaaagaa
    25741 ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac tggttatcgc gcaagacgcg
    25801 agccgcctga ctgcctgatt tcacaccgcc agctcagctg gcggtgctgt tttgtaaccc
    25861 gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg tattattatg ctgatcccta
    25921 ccggaaccag cgtcggtctg accagcgtca gccttggcgt gatccgtgca atggaacgca
    25981 aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc gcgtaccggt ggcgatgcgc
    26041 ccgatcagac tacgactatc gtgcgtgcga actcttccac cacgacggcc gctgaaccgc
    26101 tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca gaaagatgtg ctgatggaag
    26161 agatcgtcgc aaactaccac gctaacacca aagacgctga agtcgttctg gttgaaggtc
    26221 tggtcccgac acgtaagcac cagtttgccc agtctctgaa ctacgaaatc gctaaaacgc
    26281 tgaatgcgga aatcgtcttc gttatgtctc agggcactga caccccggaa cagctgaaag
    26341 agcgtatcga actgacccgc aacagcttcg gtggtgccaa aaacaccaac atcaccggcg
    26401 ttatcgttaa caaactgaac gcaccggttg atgaacaggg tcgtactcgc ccggatctgt
    26461 ccgagatttt cgacgactct tccaaagcta aagtaaacaa tgttgatccg gcgaagctgc
    26521 aagaatctag cccgctgccg gttctcggcg ctgtgccgtg gagctttgac ctgatcgcga
    26581 ctcgtgcgat cgatatggct cgccacctga atgcgaccat catcaacgaa ggcgacatca
    26641 atactcgccg cgttaaatcc gtcactttct gcgcacgcag cattccgcac atgctggagc
    26701 acttccgtgc cggttctctg ctggtgactt ccgcagaccg tcctgacgtg ctggtggccg
    26761 cttgcctggc agccatgaac ggcgtagaaa tcggtgccct gctgctgact ggcggctacg
    26821 aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt cgctaccggc ctgccggtat
    26881 ttatggtgaa caccaacacc tggcagacct ctctgagcct gcagagcttc aacctggaag
    26941 ttccggttga cgatcacgaa cgtatcgaga aagttcagga atacgttgct aactacatca
    27001 acgctgactg gatcgaatct ctgactgcca cttctgagcg cagccgtcgt ctgtctccgc
    27061 cagcgttccg ttatcagctg actgaactgg cgcgcaaagc gggcaaacgt atcgttctgc
    27121 cggaaggtga cgaaccgcgt accgttaaag cagccgctat ctgtgctgaa cgtggtatcg
    27181 caacttgcgt actgctgggt aatccggcag agatcaaccg tgttgcagcg tctcagggtg
    27241 tagaactagg tgcaggcatt gaaatcgttg atccagaagt ggttcgcgaa agctatgttg
    27301 gtcgtctggt cgagctgcgt aagaacaaag gcatgaccga aaccgttgcc cgcgaacagc
    27361 tggaagacaa cgtggtgctc ggtacgctga tgctggaaca ggatgaagtt gatggtctgg
    27421 tttccggtgc tgttcacact accgcaaaca ccatccgtcc gccgctgcag ctgatcaaaa
    27481 ctgcaccggg cagctccctg gtatcttccg tgttcttcat gctgctgccg gaacaggttt
    27541 acgtttacgg tgactgtgcg atcaacccgg atccgaccgc tgaacagctg gcagaaatcg
    27601 cgattcagtc cgctgattcc gctgcggcct tcggtatcga accgcgcgtt gctatgctct
    27661 cctactccac cggtacttct ggtgcaggta gcgacgtaga aaaagttcgc gaagcaactc
    27721 gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg tccgctgcag tacgacgctg
    27781 cggtaatggc tgacgttgcg aaatccaaag cgccgaactc tccggttgca ggtcgcgcta
    27841 ccgtgttcat cttcccggat ctgaacaccg gtaacaccac ctacaaagcg gtacagcgtt
    27901 ctgccgacct gatctccatc gggccgatgc tgcagggtat gcgcaaaccg gttaacgacc
    27961 tgtcccgtgg cgcactggtt gacgatatcg tctacaccat cgcgctgact gcgattcagt
    28021 ctgcacagca gcagtaatct cgtcatcatc cacagctttg cgctgcggat atctgaaccg
    28081 gaaataatca ctatttccgg ttttttattc tcttaatttg cattaatcct ttctgattat
    28141 cttgcttaac tgcgctgcat caatgaattg cgccatctca ctttgcatac ttaccacttt
    28201 gttttgtgca agggaatatt tgcgctatgt ccgcaatcac tgaatccaaa ccaacaagaa
    28261 gatgggcaat gcccgatacg ttggtgatta tcttttttgt tgctatttta accagtcttg
    28321 ccacctgggt agttccggtg gggatgtttg acagtcagga agtgcagtat caggttgatg
    28381 gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt tcgcattctg actaacgaag
    28441 caggcgaacc tgagtatcac cgcgtacagc tgttcacgac gggcgatgaa cgcccgggcc
    28501 tgatgaactt cccgtttgaa gggttaacct caggatcgaa atacgggaca gccgttggca
    28561 tcatcatgtt tatgctggtg attggcggcg cgtttggcat tgtgatgcgt acaggaacca
    28621 ttgataacgg tatcctggcg cttattcgcc atacccgcgg gaatgaaatt ctctttattc
    28681 ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt tggtatggga gaagaggccg
    28741 tcgcctttgc cattatcatc gcaccgctaa tggtccggct gggctatgac agtattacca
    28801 ccgtcctggt gacttatatt gccacgcaaa tcggttttgc cagttcgtgg atgaacccgt
    28861 tttgtgtggt tgttgctcag gggattgccg gcgttccggt gctttctggc tccgggttgc
    28921 gcatcgtggt gtgggttatc gccactctga ttggcctgat ctttaccatg gtgtacgcct
    28981 cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga gtccgaccgc ttctttcgtg
    29041 aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg tgactggctg gtattgattg
    29101 tcctgacagc cgtaatggtc tgggtgattt ggggcgtgat cgttaatgcc tggtttattc
    29161 cagaaattgc cagccagttc ttcaccatgg gtctggtgat tggcatcatc ggcgttgttt
    29221 tccgccttaa cggcatgacg gttaatacca tggcttcatc ctttaccgaa ggggcgcgaa
    29281 tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg gattttgctg ctggttggta
    29341 atggtgaagc aggtaatgcc agcgtgttaa atactatcct caacagcatt gccaatgcca
    29401 ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt gctcttccag gctgtattta
    29461 atttcttcgt gacgtccggt tctggtcagg cggcattaac tatgccgtta ctggcaccgc
    29521 ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct ggcgttccag tttggtgatg
    29581 gcttcagcca catcatttac ccaacctcag cttcgttaat ggcaacgctc ggtgtgtgcc
    29641 gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct gcttggactg ctgtttatta
    29701 tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta ccactaaaaa tgttaagagc
    29761 cgcaattgcg gctctttttt cattctgccg tttcagtctc tacggcttca tttttggcat
    29821 tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg agtgaactca tcgcagcgtg
    29881 cggtgatttc ttccggcgtc agccagcaaa ctccactgac ttcatcttcc tgtagggcga
    29941 agggaccgtg agagacgcaa ctgaacaacg cgccccagac acggcaattt ttatcttcga
    30001 aatagaactg cccgtgctcg gcaaagggga caccggcaat gcccaactct tcttccgctt
    30061 cgcgacgcgc ggattccagc agttgctcat cggcctgaac tacaccgcct gcggtcgcat
    30121 ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg ctggaccaga attttgccca
    30181 tgccatcatg cacgacgatg taagttgcac gatgacgcag acactgtgcc cgcatttgtt
    30241 cccggctggc ttgtgcaatg acttcgttct cttcattgac aatatccacc cattcagtac
    30301 ttgccaaacg acgctgttcc attatcagga aaccttcttt ttctggcgct cttacggcgc
    30361 attttgagtt gtgggtaaat tacggattaa tcgcgacctg cgcaatgata ctttgatcat
    30421 tgagtgcgat tacactaagt acgtcattat ccagcatgcc ataacccgcc ggattaccgc
    30481 ctttcggaat actcaccgag ccggggttga agtggaaaat ctccccccgc tgttctgcca
    30541 ctggcagatg ggtatgaccg tacaccagca catcgttctg gtttaaagca ggtagatttt
    30601 ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg tttttccagt aatacctgtt
    30661 gccacggcgc ggttatcggg aaatgcagca tcatttgatc cacttcgctg tcgcagttgc
    30721 cacgcacagc gataacctta tgtgccactt cattaagccg ttcagcgact ttggctggcg
    30781 cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa cacgtcgcca aggatcacca
    30841 gccactgggc accgctttgg acaaacaact ccacaacacg ttccgtcgcc ggtaacgacc
    30901 catgaatgtc cgatgcaaac atcagtttca tcactcactc ctcgtcgaaa aaacgtaagc
    30961 tatgatactg gattctgtcg ttgctatcag ccagattgct tcgccgaaag tgcaataaaa
    31021 cgctggacag acgctcgctg ccactggaat gtcgcataat ccaccaggcg ttctggcacc
    31081 tcatcgccat gtagtaccag gcggttaatc attagcgcca gatcagtatc agcaatgcac
    31141 cattcaccaa ataaattcgg ctgacccagt gctaacaaat gttctgccat cgcgaacagt
    31201 ttctctgcac tggcttttcc ctcggccgtt agtggcgctt ttttcgcccc cgcaaagaca
    31261 acatccgtcg gacgctcttc gcggatgggc atcagatcgc tgcgcagcca ggcctgaatc
    31321 tgtcgtgcac gcgcacgatt ttctaaatca agcggataaa tacgttccca ggtcggtggc
    31381 gcaaatcgat cttccagata ctccgcaatg gcagaagatt cactcaactc aaaatcatcg
    31441 atttgtaata acggcacacg gcgtgtctga ccgtaacctt gccacgtcgg ctgcaaatgt
    31501 tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca ggcctttttc ctgcaacgcc
    31561 acccaggcgg ataacacata aggggagaaa aagtgggcat ctgaccaaag cgtgatagcg
    31621 ggtttactca ttatgtcctc ggcaataaag ggggctggct atcaaactat agagcctttt
    31681 tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg ctttctatac ttgtggtgtt
    31741 cagatgatta aacaaccgga gctgcaatga tcgatctcta tttcgccccg acacccaatg
    31801 gtcacaaaat tacgctgttt ctcgaagaag cagagcttga ttatcgcttg attaaggtag
    31861 acttggggaa aggcggtcag tttcgtccgg aatttttgcg catttcgccc aacaacaaaa
    31921 ttccggcaat tgttgatcat tctcctgccg atggcggcga accgttaagc ctctttgagg
    31981 ctgctgccat tttgttgtat ctggctgaga aaacaggact ctttttgagt catgaaacgc
    32041 gtgagcgcgc cgccacatta cagtggttat tctggcaggt aggcggactg gggccgatgc
    32101 ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac cattccttac gctattgaac
    32161 gttatcaggt tgaaactcag cgtctttacc atgtactaaa caagcggctg gaaaactcgc
    32221 cctggctggg aggcgagaac tacagcattg cggatattgc ctgctggccg tgggttaatg
    32281 cctggactcg cctgcgaatt gacctcgcaa tgtatccggc agtcaagaac tggcatgagc
    32341 ggatccgttc gcgccctgcc accgggcagg cactgctaaa agcacaactc ggtgatgagc
    32401 gttcggatag ttaacagaaa caggttctcg tgtattattt catcctaagt aaaacaacgg
    32461 agaacctgca atggcacaac ctgccgctat tattcgtata aagaaccttc gtttgcgtac
    32521 gtttatcgga attaaggaag aagaaattaa caaccgtcag gatattgtta tcaatgtgac
    32581 gatccactac cccgccgata aagcgcgcac tagcgaagat atcaacgatg cgctgaatta
    32641 tcgcaccgta acgaaaaaca ttattcagca tgtagagaat aaccgtttct ctttgctgga
    32701 aaaattaact caggatgtgc tcgatatcgc acgtgaacat cactgggtga cgtatgctga
    32761 agtggagatc gataaactgc acgcgctgcg ctacgccgat tcggtatcca tgaccttaag
    32821 ctggcagcgt taatcgccat atcgggaggc tgcatgaaca tagtgatcac cggagggacg
    32881 ggattaattg gtcgccattt gattccgcgt ttgctggagc tgggccatca aattacggta
    32941 gtgacgcgta acccgcagaa agccagttcc gttctcggcc ctcgggtgac actatggcaa
    33001 gggcttgccg atcaaagcaa cctcaacggc gttgatgcgg taatcaacct ggccggagaa
    33061 ccgattgctg ataaacgctg gactcacaag caaaaagagc gtctctgcca aagccgctgg
    33121 aatatcacgc aaaaactggt cgatttgatt aatgctagcg acacgccgcc gtcggtactc
    33181 atttccggct cggcaacggg ctattatggc gacttaggtg aagtggtggt taccgaagag
    33241 gaaccgccgc ataacgaatt tactcataaa ctctgcgccc gctgggaaga gattgcctgc
    33301 cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa ccggcgtggt actggcaccg
    33361 gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc ttggcctggg cgggccgatt
    33421 ggttccggtc ggcagtatct ggcctggatt catattgatg atatggtcaa cggcattctc
    33481 tggctgctgg ataacgagct gcgcgggcca tttaatatgg tttcgcccta cccggtacgc
    33541 aatgaacaat ttgcccatgc gctcggtcat gcgctgcatc gcccggctat tttgcgcgtc
    33601 cccacaaccg ccattcggct gttaatgggc gaatcttcag tactggtatt aggtggacaa
    33661 cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt ttcgctggta cgatttagaa
    33721 gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc aaacatccgc cagttaactc
    33781 cctgtgttac aggattagtg gctttgcgcg ataagatcgt ctggtgaaag tcgggtcacc
    33841 atcataacta actctctgtc taaacctcta tccagcatct cctgagcaat acgcagggct
    33901 tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc tgtcagcaat ggtcatcagt
    33961 ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa taaatgcacg gaaacgctgg
    34021 gcatcccctg tttgtaatac gtaattaaac agggctttta gctgtctgtc attagtgttc
    34081 cctgtaacta gcagcgaaac aatttggtcg actaatccca acagatcgcg ctgacgaata
    34141 tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt gcataatctc gtcatccggc
    34201 accacggtaa tatccaccaa cggaaacgct gatgaatata ttttgcgggc tatagcaggc
    34261 tcggcaaatt catcaagcca gcagagtgaa taaggataag gacttctgca accatgataa
    34321 aacagcatcg ggatcaccaa tggaagctct ttatagcccg catcaagatg gttttgcatt
    34381 gccgcaatgg aataacgcat catgcgaaaa gccattaatt cttccggctt actttggtgc
    34441 tctatcacta cataaatata acccactccc tcctgcgttt tcacagacca caagaggtcg
    34501 gaataatatt gccgcaggtc ttcatcaata aaactgtttg gttctagttt aagcgtcgtt
    34561 aaatcacaca gtttgcgcag cggcgcggga agatgaatat taataaaatt ccgcgcggtg
    34621 tctggatggc gtaaaaaaga tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt
    34681 gtcatggcgt tccgtcaccc ctcaaattta agatgacgcg acaataacca cgattaacac
    34741 ccaatgcagc aggcagtttt tatctttgga cagcgaattc agaagtattt ttagcggact
    34801 gcaactttta catttcagcg gaagctgact cgcaaattcc ccttgaaaat actccggcag
    34861 cgttagccac cggagaggaa attatttcag cgatccctta aggaactgtt gcagacgagg
    34921 gctttgcggg ttgccaaata actgctccgg cgcgccctct tcttctattt tcccctgatg
    34981 gaggaaaatg acgtgagtag aaacatggcg agcaaagccc atttcgtgag tcactaccac
    35041 catggttttc ccctcttctg ccagttgctg cataatacgt aacacttcgc ctaccagttc
    35101 aggatcgagc gccgaggtag gttcatcaaa cagtaaaact tctggttcca tcgccagcgc
    35161 ccgcgcgata gaaacacgct gttgctgacc gccggaaaga tgcaccggat atttcccctg
    35221 cgcacgttcg tctatcccga ctttcgccag atacttcacc gcccgctcgc gcgcttcctg
    35281 cttgctcagg cccaacacct gaatcggcgc ttccatgacg ttttccaaca ccgtcatatg
    35341 gctccagaga ttgaagtgct ggaataccat cgtcaggcgt gtgcgcagta agcgcagttg
    35401 atttttatcg gcgactttga gttgaccgtc tttgtcgcgc accagattga tcgtctggcc
    35461 attgaccacg atcgatcctt cactcggttt ttcgaggaag ttaatgcagc gcagaaaggt
    35521 acttttcccc gatcccgacg atccgatgat gcttattaca tctccggcat tcgcttgcag
    35581 tgagacccct ttcagcactt cgtgttcgcc atagcgtttg tgcaaatcga taacgtttaa
    35641 tttattctcg gacatcgtat tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc
    35701 gtttttccgc tctgcgaaag aggctgatca ggacataaga gatgattaaa tagagcaccg
    35761 cggcaatgcc gaaggcggta aaaggttgat acgtggcggc gttaatatcg cgggctattt
    35821 tcagcagatc cggcacagtg gccgtaaacg ccagcgcagt agagtgcagc atcaggatca
    35881 cttcgttgct gtatgccggt aacgcaatac gcagcgcaga aggcaaaata atgcagcgat
    35941 acattttaaa ggtcgagaag ccataggctc tggcggcttc aatttcccca tgcggaaccg
    36001 aacggattgc cccagcaaaa atctcggtag tgtaagcgca ggtgttaagc gtcagcgcca
    36061 gcacggtaca gttcaggcca ctgcggaaga aagcgttaag gaattcggtt cccttaacaa
    36121 tctcaagcgt gtacatgccg gaatagaaca ccagcaactg aacatacagc ggcgtaccgc
    36181 gaaaaatata ggtaaataac cagattggaa actggatgta tttattactg gagacacgac
    36241 caatcgccag aaacagcgcc aggactccgc ctatcactac cgacaaaata agcagccaca
    36301 gagtgatcgc cacaccagta aagcgataac cgtcggtcca cagcagcggt ttccagtatt
    36361 catgtaagat ttcgatcaca ggtcagccct cttcacaccc acggagtagc ggcgctcaag
    36421 gaacagcagc acaccattgg aaacggtggt gaaaaccagg taaatcacgc cacagacgat
    36481 ggcgaaatag aacggttccc aggtactttt gcctgccagc tgcgtggctt tgaccacatc
    36541 ttccaggccg agtaacgaaa ccagtgcggt agatttgagg atcacctgcc agttgttgcc
    36601 aatgcctggc agggcgtaac gcatcatcgc cggaaacatg atccgccgaa acacttgccc
    36661 gcgagtaaaa ccgaacgccg tcgccgcctc tatatgccct ttcggcactg ccataaacgc
    36721 gccacggaac gtttcggtaa aataagcacc gtagataaaa ccgagagtga taataccagc
    36781 gaccatcgga tcgatatcaa tctgcccgac gcccatcgcc tccgtcacca tgtttagcgc
    36841 aatctgcaaa ccatagaaaa tcagcagcat caacaccaag tccggcacgc ctcgaatcag
    36901 cgtggtgtac ccttcgaaaa tcagccccga aagccgattt tgcgagagct taccgccagc
    36961 gccaattaaa ccgatgatta cagcgagcac tacagagctg atagccagct ccagcgtgac
    37021 gagcgcaccc tgtaaaataa cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg
    37081 tgtggtgaat taccgcctct tgtctccctc cggttacccg aagggagcgg gctttctttc
    37141 accgatggcg attaaccacc ataaacatca aaatcgaagt actttttcgc taatttctcg
    37201 taagtaccgt cagcgcgcat ttcggcaaag gctttgttca gtgcctcgcg cagttcgtta
    37261 tcttctttac gcaggcccat gccggtccct acgccaaaca gtttttcatc tttaacagac
    37321 ggaccaccga atttgtaatc tttaccgacg ggttgtttga ggaaaccttc gctggcagcg
    37381 acctcatcct ggaacgcggc atcaatacgt ccggcagtca ggtcagaata aatgttgtcc
    37441 tgcccctgat acgagacgat ttcaatgcct tttggtgccc agtgttcatt accgaacgtc
    37501 tcctgggtgg tgccctgcaa tacgcctacc cgtttgcctt tcagcgactc gactgtcggc
    37561 tgaatgtcag aatttttcgc caccaccaga cgagaatcgg cagcgtacag tttgtcggtg
    37621 aaggcaattt cttgctgacg tttttccgta atggaaagcg atgacatgat ggcgtcaatc
    37681 ttcttcgctt ttaaggacgg gattaacgca tccagcgggt tttcgacaaa cgtacattgc
    37741 gtattgatgc gcttgcacaa ctccttcgcc agctcgatat cgaaaccaac cagttcgcct
    37801 tgtgaatttt ttgattcaaa tggcgcatag gtcgggtcgg taccgatgcg gatgttttgc
    37861 ggaattgcag caagtgccgc agttgcgctg gagaaggcca gaaccagaga gagcgatagc
    37921 accagttttt tcataatgtc ctcaactgac agtcttttta tagggaatat ttacaggttt
    37981 gttacactta tcgtgccata aaattgacca tcaaggcaaa atatcccgcc ccgttagacg
    38041 tattattttt cattaaaaat gcttaagtat gcatttcaat gcaccacgat agtgcaacaa
    38101 acctggcgtg caccatgatg atcattccat caggtacagc ttcccagcga cgtatcagtc
    38161 accgtagaca ttaaagtcga aatacttttt cgccatcttg tcgtaggtgc cgtcctgacg
    38221 cagctcgcca agcgccttat tgaaggcagc cgtcagttca gcataatctt tacgtagccc
    38281 tacaccggtg ccatcaccga agtatttttt gtcttttact gatgagccag caaaagcgaa
    38341 atctttacca gcaggttgct tgaggaatcc ttcgctggca gcaacttcat cttgtaacgc
    38401 agcatccaga cgtcctgcag ccagatcgga atagaccaaa tcctggttgg cataggccac
    38461 cacatccacg cctttactac gccaggtctc gttagcgtaa gcttcctggg ttgatccctg
    38521 cagcacacca acatgtttac ctttcagtga atccagcgtt ggctgaatcg gtgaaccttt
    38581 ggcagcaatc aaacgagaat ctgcggcgta cagcttgtcg gagaaggcaa tctcctgctg
    38641 acgtttatcg gtaatggaaa gcgacgaaat aatagcgtcg atttttttcg ctttcagtga
    38701 ggggatcagc gcgtcaaagt cactggcaac ccaggtacat ttcacctgca tccgtttgca
    38761 catctcgtta ccgagatcga tatcaaagcc aacaaaatca cctttagcat ctttcgacga
    38821 gaacagcgca taggtggtat cggttccgat acgtaccgtc tccggtagcg ccgcatagct
    38881 ggaagccgct gcggagagac cgactaataa agacagagcg agaatcgact tcttcataca
    38941 taaccctcaa gtgaaaatgg cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctc
    39001 tttcatgcag gtcttatgcc atcttgacga caacagtaac attcaacgtt aaatatgtta
    39061 ataagacatt gcattattgt cctgaagttg aagatagcag gtatagcggt tgaatcgcag
    39121 cgtttcgatt gaatggcaga aacaaaatgt cgaggatttg atcgcggttg caaaattgcc
    39181 ctgaaacagg gcaacagcgg agttatgcgc cctgccagcg ggcaaagaga tcttcaggaa
    39241 gggttattgc aaactggtca agaacacgat taaccgtctg atttatcaca tcatcaaggg
    39301 attgcgggcg atgataaaac gccggaacgg gaggcataat caccgcaccg atttctgccg
    39361 cctgagtcat taaacgcaga tggcctaagt gcaatggtgt ttcacgcacg cagagcacca
    39421 acgggcgacg ctctttcagc accacatctg ccgcacgggt cagtaagcca tcagtatagc
    39481 tatggacaat gccggacagg gttttgattg aacagggtaa aatcaccatc cccagcgtct
    39541 ggaaagaacc ggaagagatg ctggcggaaa tatcgcgcgc atcgagcgtg acatcagcta
    39601 atgcctgcac ttcgcgcagg gaaaaatccg tttcgaggga taaggtctgg cgcgccgcct
    39661 ggctcatcac cagatgcgtt tcgatatctg tgacatcgcg cagaacctgt aataagcgca
    39721 cgccataaat cgcgccgctg gcaccgctga tgcctacaat gagtcgtttc ataaatagtt
    39781 gccctgtcag acttgcaggc agactttgca gtatttcggc gggagttgca agtcagggtg
    39841 ccagaccggc accctcagcg aaggcatcat ccttcgttat gcatttcgag attttccact
    39901 tcgttctgac gttgcactgc tttggcgtca tcattacgta acgtatcgag gaaatcgagg
    39961 tagccctgat caacatcttt ggtgacgtag acgccgttga acaccgagca ttcaaactgc
    40021 tggatatccg gattttcagc gcgaacggcg tcgatcagat cgttcagatc ctggaaaatc
    40081 aacccgtcag caccgatgat ctggcgaatt tcatcaactt cgcgaccgtg agcgatcagt
    40141 tccgtggcgc tcggcatatc aataccataa acgttcggga agcgaatttc cggtgccgca
    40201 gaagcgaggt acactttctt cgctccggct tcgcgtgcca tctcgataat ctgctcagaa
    40261 gtggtgccac ggacgatgga gtcgtcgacc agcaggacgt ttttatcgcg gaactcggcg
    40321 cggttggcat tcagtttacg gcgcacggac ttacgacgca gctgctggcc cggcatgata
    40381 aaggtgcggc caacatagcg gtttttaacg aagccctggc ggtacggttt gcccagaata
    40441 cgagcaattt ccagcgcgat atcacacgag gtttctggga tcgggatcac cacgtcgata
    40501 tccagatctt cccattcgcg ggcaattttc tcgcccagtt tcgtgcccat attcacacgc
    40561 gcgctgtaaa cggaaatttt gtcgataaac gagtccgggc gggcaaagta tacatactca
    40621 aacaggcacg gattgctgac cggattgtca gcacattgac gggtaaacaa ctgcccttct
    40681 tcagtgatgt aaatcgcttc gcccggcgcg acgtcacgca ggaaatcaaa gcccagcgta
    40741 tcgagcgcta cgctttcgga agcgaccata tattctgtac ggttctcgtc aatatcacgt
    40801 tttcccagta ccagcggacg aatcccgttt ggatcgcgga aagcaaccat accgtggccg
    40861 ataatcatcg ccacacaggc atacgcgccg cggattaagc ggtttgtggc agcaatggca
    40921 gcgaaaatat tgtcggcttc cagcgggtag tggcggaagt tgtccagctc gctggcgaag
    40981 atattaagca gaatttccga gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac
    41041 agttttttac gcaactcgtg agcgttggtc agattgccgt tgtgggcaag cgtaatgcca
    41101 tacggggagt taacgtaaaa cggctgcgct tcagaggcgc tggagctgcc agccgtaggg
    41161 taacgcacat gaccaatgcc catattgccc tgcaaacgct gcatatggcg agcttcaaat
    41221 acatcgctca ccagcccgtt cgctttacgc aaacggaagc agttattggc atctatggtg
    41281 atgatgccgg cggcatcctg accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac
    41341 tggttaaccg gcataacacc ggcgataccg acaataccgc acatacgtct ttttcctcgt
    41401 taagccacat ctcagagcac ttacgctctg ggcaagaaac ttgacgagct ttgcagataa
    41461 tcaaaaaacc atctgatgat aaaactgaat tgcgggatca gctgtgattt gctccagtct
    41521 tcacttttcg acaccccggt aaaggagtcg agaaagaaga gaatggcggc aacaatcaac
    41581 acaccgcgca acgcaccgaa acagacgccc agcacccgat cggtgcctga caaccccgtt
    41641 ttctccacca actggcctat cacgaagttc acgatagcac caacgatcag ggtagcgata
    41701 aacagtaccg cgatggcaat cccatttcga accagttcgt cttcaaagcc cgtaaaccag
    41761 actgacaggt aagtgtagta atgactggca acaaagaaag cacaacccca tgtcaccagc
    41821 gataacgctt cacgaacaaa gccgtggatc aggctaacca gagaggaaaa agcaatcacc
    41881 gcgattatgg cgtaatcaat ccagaccata tgtgtcccac gattttacgc cctgtcatcc
    41941 tgttcggggc gcattctaac agaaaaagaa aacgtttgcg tagggatttc cttcccgcgc
    42001 atcaataaaa atggcgctga aaaaatattc aacgccatcg actttttatg cctttgcggc
    42061 atcgtgcaat gcgtgtcgga tgcggcgtaa acgccttatc cgacctacgg ttctaacact
    42121 gcccaagcct gataagacgc gccagcgtcg catcaggcaa gactgtatta attcggcgta
    42181 tagcccatta ccacgccact taagccagaa agttgcttca actcacccag cgaacctttc
    42241 agcttatctt tcgaggcatc cggcccaacc agaatacggg taattttacc ctgcactggc
    42301 gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac aatctcattc
    42361 actttatcgg catttttcag cgcacccagt tgcacaacat aggctttacc cgttggcgct
    42421 gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtac ttcaaccttt
    42481 ggtttaggcg gctccaccgg tttcggcttc ggtggggcga ccggtgcagg ttccggttca
    42541 aactcggtgt tattggctgc aatagtggcc ggatcgagcg acggtgctgc ggcatcacct
    42601 gcccgcacct cttccgctgc gccttccggc ggctgcgtcg gcaacgcctg ggtggcggct
    42661 ggcatcatat caggctcatc acgatcgccc gctttcggca ccagcgggat agccgcgaac
    42721 tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat cacccccagc
    42781 gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac cgcttctcct
    42841 cgcgtcaatc acttccatga catgtgcgac cgtgtggaaa gaaccacaca ccagcacggt
    42901 gtcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgcaacgc tatcaaatga
    42961 tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg gcccttccag
    43021 tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc cggcaatatc
    43081 tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta gcgctttcat
    43141 acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa taacgcgtgg
    43201 cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc cgtcgcgaat
    43261 ggcattttca ctgacttcca gcccgctggc acgcagtgcc gccagcgccg ttgcggcatt
    43321 cggttgcggg acaagcggca acggcagatt ttccagcgtg ccgtgagcat cgctaaacgc
    43381 ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta acagtgcacc
    43441 tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga caattgccgg
    43501 tttttcgctg cggaagatgc ctgctttctc gagaccaata ctttcgcgat ctggacccag
    43561 ccagtcggta tgatccagcg caatactggt tactaccgcg acatcggcat cgacaatatt
    43621 ggttgcgtcc agacgaccgc ccagccctac ttccagaatc accacgtcaa gttgtgcctg
    43681 cttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg aaatatcacc
    43741 gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccggca attcctggcc
    43801 ctgcacacgt acgcgctcgg tataacgcac cagatgaggc gaactgtaga cgcccacttt
    43861 gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt tgccattcgt
    43921 acccgcaacg gtaaacacaa atggcgctgg tttcaggacg ccaagacgcg ccgcgacctg
    43981 gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca gataagaaag
    44041 ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg tatccgctga
    44101 ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta tcagttatca
    44161 ggcctcaggt tcctgatccg gtaccggggg taccactacg ccttcacgcg gcgcttcagg
    44221 attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca ggcgcatttc
    44281 cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac tgcgctggaa
    44341 tctaggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg caaagccgat
    44401 taacgcttta ggttcagcga tgttgagatc gcccagcatg gcgaaacttg cagaaacacc
    44461 gcccatcgtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct gcatttttgc
    44521 cagtgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct gcatacgtgc
    44581 gccaccagag gcggagaagc agatcagcgg gcagttatct tcctgcgcct gctcaacggc
    44641 acgcacgaaa cgtgcaccca caacagaccc cattgaaccg cccataaagg cgaactcgaa
    44701 tgccgcagcg acaaccggca ttccatacag agtgcctttc atcaccacca gcgcatcttt
    44761 ttcgccggtt tctttctgcg cagatgccag acggtcttta tacttcttgg agtcacgaaa
    44821 cttcagctca tctttcggct caagctcgct acccagctcc acaaggcttc cttcatctaa
    44881 caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact tcggacagac
    44941 ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat cacacttagt
    45001 ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt taattcgttc
    45061 aatccagctc attagggacc tttctgtctg aacctggttc gatgccagtt ttatctttgg
    45121 ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt aaaacataac
    45181 agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta ttttgcggcg
    45241 cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc ccggcaaaat agaaaccaca
    45301 ataatcccga cgatcagcag cttaaggtta tcctgaacca tcgggattgt accgaagaaa
    45361 tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc cgatcacgtt ataagcagcg
    45421 aaatgacggt acgacatgtg gcccattccc gcaacaaacg gggcgaacgt tctgacgatc
    45481 ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcataaaa ctgatgggtt
    45541 ttgtcgagat aactgcgacg gaaaattttc gaattgggat tactgaataa cttttcaccg
    45601 aacaaccgcc cgatagtgta attgaccgcg tcacccacaa tcgcggcgat cagcatcagc
    45661 actaccatca tatggacgtt gagatcgttg gtttccagtg atgccaacgc tccggcaaca
    45721 aacagcaatg aatcacccgg taaaaacggc attaccacca gaccggtttc acagaacaaa
    45781 attaagaaca aaatggcata aacccagacg ccgtactccg cgactagctc tgccagatgc
    45841 acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt tgcctatgcc
    45901 ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat gttccgagca
    45961 ttattctcgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa gatcataccg
    46021 gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg ctgccgccag
    46081 cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt tgtgggcacc
    46141 gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg cattcgcttt
    46201 gatatcaacc accacataag gaccgtgacg cgtgacatta atatgcataa cgttgcgcca
    46261 cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct cgcccagcaa
    46321 gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat gggttacccc
    46381 tttactcagt accgccgggc gcagccgatg attgtagatg atgtagcgat aacggcgagc
    46441 cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac gcacggcgat
    46501 gtcaccaggt aaattcgcat ttacgcccaa ggtccacgcc gcgtctttgc gctgcgcggt
    46561 tgtttcgaaa tgcaccactt gcccggtacc gtgtacccct gcgtcagtac gcccggcgca
    46621 gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct tctcctgcac
    46681 actgcggact tcattctgcc gttgccagcc gtaatactta ctgccgtcgt actcaatgcc
    46741 cagcgcaatt ttataaactg gcagttgttg ctggtcggac attagtacag atactcctgc
    46801 accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac gttatcggcc
    46861 accgaccaga actggacttg ctccggcata ccgtagtcat tacgcacgca gccaacagaa
    46921 agatgcggcg tacccgaagc atcccctacc tgagtcggga attcgttctc ttcagagagc
    46981 acaatatctt cgccctgagc aaacgcatca cgcgcttctt ctgccgccag cggacgcagt
    47041 gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac gacgcttgcc
    47101 gaaatcatca gcccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat acgacgttct
    47161 tcacgcacgc taccttcgct atccggcaac aacggcagca tattgaacgc cagttgacga
    47221 ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca atttcgcact ctgccccgct
    47281 aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt aacactgata
    47341 cgcgacaaac cgccctgatc gattaacggt ttcagtgccg ccagcagctg gctggtcaga
    47401 ctgtctggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa cgggtttact
    47461 tccggcacca ccagcggtac gtcgggttcg agagcaaaca gtccgctgct gtcgatcacc
    47521 aggcaacctg agttggtcgc ttcttcaacc caggcagcgg tagcttcttt gcctgcgaca
    47581 aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg catcctgcac ggtgattgtt
    47641 ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc ataaatttcc
    47701 ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccat agcgccagtt
    47761 gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag aaatagcaaa
    47821 gctccctgcc aatgttacag cagagagcat gaagaagaga ttaacgtgcc ggatgatgaa
    47881 cggcgttaaa acccagttta cacagcaatg atgccgcact ggcgtcatca caaattacat
    47941 acagagatga ccattcacgg cgctcaagat agtttttgcg cagtttatcg aactcacccg
    48001 gtatcccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac accaaatgca
    48061 ccagcctttt cagcgtcggt tgatcgagca gaccatgcag cgtaatgcgg ccaaactcag
    48121 gcgcgggcag caacgtatcc agcgcaacgt gttgttcatg cccaataaac ttgctatagg
    48181 cttcaaacac ttgcgtcgtg ccacgcgctt taccctccag ggtatagcct gcgatatgcg
    48241 gcgtgccgat atccactttt gtcagcaatt ccacattaag ttccggctcg ccttcccaga
    48301 catccagcac cacgcttaac ttctggcctt cattcaggca ggtcagcagc gcggtattat
    48361 cgacgactgc gccacggcag gcgttaatca gaatcgctcc gggcttcagg ctacggatca
    48421 gtttttcatc cgccagatgt agcgttttgt acggaccatc tttaaagagt ggcgtatgga
    48481 aagtcagaat atccgcgcgc tggactaact catccagcga gcggaaatca ccctcatccc
    48541 cacggtcggc gcgaggcgga tcgcaaagta aggttttaat ccctaacgct tccagtcgcg
    48601 cctgcaatcg acgtccaacg ttaccaacgc ccacgatccc caccgtacgg tcgtgcagtg
    48661 aaaatccatc gcgttcggca agcatcagca gggaggagaa aacatattcc accaccgcaa
    48721 tcgcattaca gccaggtgca gcggaaaaac caattcccgc ctgcttcaac catgcttcat
    48781 cgacatggtc ggtccccgca gtggcagttc caacaaattt aatgggtttt cctgccagca
    48841 aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct gccagttgag
    48901 cgacggggat tggacgcccg ggaaccgcga tcacctcacc caaacggcta aataagtcgc
    48961 gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct gtatgagacg
    49021 agagttaacc ggacaagtat gccataatct cgcggccagg catacttgcg aagatttcag
    49081 gtataaggat acgtaatgat acaacctatt tccggccctc ctcctgggca gccaccaggt
    49141 cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca gcaacgtact
    49201 tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacaacaaag agcagaactg
    49261 tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct ttcgcgtcac
    49321 ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa aagccattct
    49381 gcccgccagc ttttagcgca attaggggag tatttacgtc tggggaatca tcgtcaggcg
    49441 gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct ctcaccggag
    49501 caattaaaaa ccattctcac cctgttgcag gaagggaaga tggttattcc gcaaccacag
    49561 cagcgcgagg cgaccgaccg tcctttatta ccggcggagc acaatgcgct aaaacagctg
    49621 gtgaccaaac ttacggcggc aacgggggaa cccagcaaac agatctggca atcgatgctg
    49681 gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa ccatctggtg
    49741 acctggctac aggcgcgtca gacgctaagc cagcaaaata cgccgacgct ggaatcacta
    49801 cagatggcgc taaaacaacc tttagatgcc agtgaactgg cggcgttatc ggcatatatc
    49861 cagcaaaaat atggcctttc tgcgcaatca tcgctttctt ctgcccaggc cgaggatatt
    49921 cttaatcagc tttatcaacg gcgggttaaa gggattgatc cgcgtgatat gcaaccgctg
    49981 cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg tcccgcgctg
    50041 tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggttcgtta accccgacga
    50101 aatgacagta tcgtgacaat aatacccagc accgcagaga tcgccccggc aagaaatacc
    50161 gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac gccgagggcg
    50221 atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga ggggacgcgt
    50281 ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc ggttaacgcc
    50341 gcgcccgcta atgcgaccca tgcacctggg gcttgccaga gcagcaacaa gcccaccgtt
    50401 tctacaagca gagagacaat cgccactttc acgccgccaa aacggtccgg catccagcca
    50461 aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa gcccgccatc
    50521 gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc aaaaccaacg
    50581 ccttgtagtg ccagccctaa ccctggtttc cagataagcc cgacaacgct ccacagcgat
    50641 ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc ccacgccagt
    50701 aagggtaata ccattgtggt gatcgccagt gcggcaaaac cgtaatggct atgaatcaac
    50761 aggccaagcg gcgcaccaac agcgagggca ccgtaaatcg ccattccgtt ccatgacatc
    50821 actttgccag agtgttttgg ccctacgatg cctaaccccc aggtcagagc gcctgtcagt
    50881 aactggcttt caccaaaccc aagaatcaaa cgcccgatga ccaacagggc aaatttgaac
    50941 ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc cgccagccag accacaagct
    51001 aacatcccct gaagcgccga acgttttgcg ccatattgat cggccagtcg cccggcgtaa
    51061 ccacgcgtca gcaccgtagc cagaaactga atcccgacgg caatgccgac catggtattg
    51121 ccatagccca gatcatgatg aacaaacagc gggataaccg gcaacggcaa ccctacggtc
    51181 atgtaggtga gaaaaaccgc aaaagagatg cggaagagcg aaaaactggc agaagatcgt
    51241 gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa ggcgaggagt
    51301 atccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggatt taacgcgtac
    51361 gtcttttcag aaggaaatcg acaaagcggg aagtttgcct agaactggtg gcgattgtca
    51421 atgatgtgaa aaagggaacc atcaggttcc cttttgcgtt agtgccggag gccgcattgg
    51481 cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac ctactccggc ctactgcgaa
    51541 ttaatctttt cagcttgcgc attaccagcg tggcgttggt gccgccgaag ccgaagctgt
    51601 tagacataac agtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc agacccgcag
    51661 cctgctcgtc cagctcttca atgttgatgc tcggggcgat aaagccgtgt tccagcatca
    51721 gcagagagta gatagcttcc tgcacacccg cagcgcccag tgagtgaccg gtcatggctt
    51781 tggttgcaga aatcgccggg ctcttatcgc cgaacacttc acggatagct gccagctctt
    51841 tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag gtaatcgatt ggggtatcaa
    51901 cgccatgcat cgccatctgc atacaacgta ctgcgccttc gccagacgga gcaaccatgt
    51961 ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc atagatgtaa gcaccacgcg
    52021 ccagcgcgtg ttccagctct tcaaccacta ccataccgcc gccgccagcg ataacgaaac
    52081 cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg tatttagtag
    52141 atagcgcacc catcgcgtcg aattcgcagg ccatttccca gcacagctct tcgccgccgc
    52201 cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta ccgatacagt
    52261 gtgcggaagt cgcacacgcg gagctgatgg agtagttaac gccatgaatt ttaaacggcg
    52321 tggcgagaca ggcagaaacg ccggatgcca tcgctttagt gaccacatac gggccaaccg
    52381 ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa cgcggggaac
    52441 cgccgccgga acctgcaatc aggccaacgc gcgggttatt ctggtaagct tccggagaga
    52501 ggcccgcatc agcgattgcc tgctccatag aaaggaatgc ataaatggat gcgtcgctca
    52561 taaagcgcac aactttgcgg tcaatgaggc cagtggtatc cagttttatg ttgccccaga
    52621 cgtggctacg catgccggaa tccttcagct cctgagagaa agtgatccct gaacgtcctt
    52681 cacgcagaga tgccaggact tcctgctggt tattaccgat gctggaaaca atgcccaggc
    52741 cagtaatcac tgcacgtttc attcaatacc tctgtaagtc gcacatagag taagtttcga
    52801 atgcacaata gcgtacactt gtacgccgaa caagtccgat cagccattta atagagatat
    52861 ttgcgcagcc ttacacacat cgctaagatc gagccaccgc ctgtaagacg agtaacttac
    52921 gtgaaacact actccataca acctgccaac ctcgaattta atgctgaggg tacacctgtt
    52981 tcccgagatt ttgacgatgt ctatttttcc aacgataacg ggctggaaga gacgcgttat
    53041 gtttttctgg gaggtaacca attagaggta cgctttcctg agcatccaca tcctctgttt
    53101 gtgatagcag agagcggctt cggcaccgga ttaaacttcc tgacgctatg gaaggcattt
    53161 gatcagtttc gcgaagcgca tccgcaagcg caattacaac gcttacattt cattagtttt
    53221 gagaaatttc ccctcacccg tgcggattta gccttagcgc atcaacactg gccggaactg
    53281 gctccgtggg cagaacaact tcaggcgcag tggccaatgc ccttgcccgg ttgccatcgt
    53341 ttattgctcg atgaaagccg cgtgacgctg gatttatggt ttggcgatat taacgaactg
    53401 accagccaac tggacgattc gctaaatcaa aaagtagatg cctggtttct ggacggcttt
    53461 gcgccagcta aaaacccgga tatgtggacg caaaatctgt ttaacgccat ggcaaggttg
    53521 gcgcgtccag gcggcacgct ggcgacattt acgtctgccg gttttgtccg ccgcggtttg
    53581 caggaagccg gattcacgat gcaaaaacgt aagggctttg ggcgcaaacg ggaaatgctt
    53641 tgtggggtga tggaacagac attaccactc ccctgctcca cgccgtggtt taatcgtacg
    53701 ggcagcagca aacgggaagt agcgattatc ggcggtggta ttgccagcgc gttgttgtcg
    53761 ctggcgttat tacggcgcgg ctggcaggta gcgctttatt gcgcggatga agcccccgcg
    53821 ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt tattaagcaa acacgatgag
    53881 gcgctaaacc gctttttctc taatgcgttt acttttgctc gtcggtttta cgaccaatta
    53941 cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt taggctggga tgagaaaagc
    54001 cagcataaaa tcgcacagat gttgtcaatg gatttacccg cagaactggc tgtagccgtt
    54061 gaggcaaatg cggttgaaca aattacgggc gttgcgacaa attgcagcgg cattacttat
    54121 ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta atgtgctgga actggcgcaa
    54181 cagcagggtt tgcagattta ttatcaatat cagttacaga atttatcccg taaggatgac
    54241 tgttggttgt tgaattttgc aggagatcag caagcaacac acagcgtagt ggtactggcg
    54301 aacgggcatc aaatcagccg tttcagccaa acgtcgactc tcccggtgta ttcggttgcc
    54361 gggcaggtca gccatattcc gacaacgccg gaactggcag agctgaagca ggtgctgtgc
    54421 tatgacggtt atctcacgcc acaaaatccg gcgaatcaac atcattgtat tggtgccagt
    54481 tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg atcagcagca gaatcgccag
    54541 cggttgattg attgtttccc gcaggcacag tgggcaaaag aggttgatgt cagtgataaa
    54601 gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt aggcaatgtt
    54661 cccgattatg aggcaacact cgtggaatat gcgtcgttgg cggagcaaaa agatgaggcg
    54721 gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg ctttaggttc tcgcggtttg
    54781 tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga accgattccg
    54841 atggatgcca gtacgctggc ggcgttaaac ccgaatcggt tatgggtgcg gaaattgttg
    54901 aagggtaaag cggttaaggc ggggtaatct gctctcgcag tgtttgccgg atgcggcgtg
    54961 aacgccttat ccaacctacg tgtgacgtgt aggcctgata agacgcgata agcgtcgcat
    55021 caggcatggt gctccagacg ccgcaactta cagctgtgac gcctgctgaa acaggttttc
    55081 ccacatatcg gtcaccagcg cctggtcacg cggcgacaat tcaccggcac caatggcttt
    55141 ttccagactc tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat caccactttc
    55201 cagttctgcg atggctaatg tcaggtggcc acgcaaatac ccactggcaa acaactcatc
    55261 atcactggca tggtcaacca tgccgtcgat taatgccaga atgcgtgatt caaactccgc
    55321 gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt cagttgagcg cttccggcca
    55381 cgggaactgt tccgccgtaa gttccggcgt gtgataataa ttttgtagtg ctttaatgaa
    55441 gcgtgccggg cgttccggaa tgccatttgt cagataatcc atcacctgcg catgtacccg
    55501 ccgttgaaac accacacgat ccggttcgaa atccccttcc agattgtcgc aactgacgtt
    55561 aaacggatat ccagccgcca cgcagaataa ccagtcgaac gcctgcggct tcacttcaac
    55621 atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc gggcaatacc agtagccgaa
    55681 atcaaccagc tcacggcgcg cttttccggc aatacaccag tgcgaaatct catgaattgc
    55741 gctggcataa aagccatgag caaagacgat tcggttatac ggtacttccg catcagcagg
    55801 aagatagatc ggttcgtcgt cgcctttaat cagacgggta ttaaattcat cggcaaagca
    55861 gctattaaaa atttcaatca actgctcgta gtggtgtgta ctgttcatta gttcatcccc
    55921 aaccagtgga ggatctcctg tccgtggcta tcgtaaagta atttggcact catcaccgcc
    55981 gagacgataa caatcatcgg gcggatcagc ttttgtcctt tgctcaacac caaccgtgaa
    56041 cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa agcccgtcgc ccaaatcact
    56101 ttgccgccga gaataaacag cagcagaccg ccaatgtttg acgttgcgtt gagtaatttg
    56161 gcgtgagccg tggctttggc gaggttgaat ccgcacagca taacgaaggc cagcgcgtaa
    56221 aacgatccgg cagccgggcc aaagaatccg tcataaaaac cgacgcagcc accggcaatt
    56281 aacgcgaagg gtaaaccgta catccggcgc tggcgatctt cttcacccag ctttggcatc
    56341 agcaagaaat agaggccgat acaaatcacc agaatgggca aaatctgccg taagacatca
    56401 gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc cgacaaaggt catggcaata
    56461 ttgagtttct gatcgcttaa actaaccact ttgcggcgaa taaagtagat agtggcggaa
    56521 atagagccgc cgcaggcttg cagtttattg gttgccagcg cgttagcggg agacattccc
    56581 gcagccatca atgccggaat ggtgagtaac ccaccgccac cggcaatcga gtcgataaat
    56641 cccgccagca tggcgacaaa aaagagaact cccagcaaca gcggagaaac cataaacagg
    56701 ctattaaacg tttccattag atcacgtgct catccagtag cgcctggcag gaaggcggca
    56761 acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt tcaaaccagc
    56821 tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa aggttgatct tcacactcca
    56881 gactatcggc agggcaacgt aatcgtacat gcatatgcgc gcgatgctgg aaccagggtc
    56941 gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag gcaaagttgt tgtttaatcg
    57001 ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc ggcgagtttg atcaagctga
    57061 aaatttctgg cttccacagc gtggagacaa cgtgtttacc gtcgcgggaa actaagtcca
    57121 gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt tttcggcagt tgcagaaaga
    57181 tatcgacatc cagtccggtc tggtggctgg catgaccgcc gttgaaacgc ccaccagcgg
    57241 gcatccccat atcgccaatc agcaccgtac ccatgcccag attgctcacc tggctactca
    57301 gacgctggat aaacatcacc agatccgggt gaccgaaata gcgacgctga tcggtacgca
    57361 tgacctgata atgttcggac tgtatcggca acgtgtcagc gccgacaata cagccattag
    57421 aaaaactgcc tatcgattgt gcgctacccg gcacaggttg ggttattttt tgccacggcg
    57481 tcgctgccag gctagcacta ctggcaagca gagccagcag cgcaatcgcg gttttgttca
    57541 ttttttacca gcgtggaata tcagtcttca catcggcatt ttgcgcccgc tgccgtaaca
    57601 ggtgatccat taaaacgatc gccagcatcg cttcagcgat cggcactgca cggatcccga
    57661 cacagggatc gtgacggcct ttggtgatca tctcaacttc ttcgccaaag cggttaatag
    57721 tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc catatgagca atgatttgct
    57781 gcccgctgct gataccgccg aaaatgccgc ccgcatggtt gctctggaaa ccgtctttgg
    57841 tgatttcgtc gcggttctgg ctgccgcgca gcgccactac gtcaaaaccg tcgccaattt
    57901 ccacgccttt caccgcgttg atgctcatca gcgcatgggc gatgtcggca tccaggcggt
    57961 caaagaccgg ctcgccaagt ccggcgggga caccactggc aacaacggtg actttcgcgc
    58021 cgatggagtc gccctctttt ttcagcgcgc gcatcagttc atctaacgcg tcgattttgt
    58081 cggggtccgg gcagaagaac ggattttgtt caaccagcga ccagtctttg atttccagcg
    58141 gaatgtcgcc catctgggtc aggcagccgc ggatttcaat accaaatttc tcggcgagat
    58201 attttttggc aatcgcccct gccgccacgc gcatggcggt ttcgcgggcg gaagaacgtc
    58261 caccgccgcg ataatcgcgc agaccgtatt tttgttcgta ggtgtaatca gcatggcctg
    58321 gacggaagac gtctttaatt gcgctgtaat cctgagaacg ctggtcggta ttttcgatca
    58381 acaagccaat gctggtgcca gtagtaacgc cttcaaaaac accggagagt attttgacct
    58441 gatccggctc gcggcgctgg gtggtatagc gcgatgtccc agggcgacga cgatcaaggt
    58501 catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg acgatgcagc
    58561 cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg aaagagttgt ccaattgtgt
    58621 ttccagccat cacggctccg ttattgttgt gtttgcgtgt ttacttaatc tttataaatc
    58681 gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga gcataaacac accatcgccg
    58741 ccgttatcaa actccagcca ggtgaacgga acatcgggat attgttccat aagatgtacc
    58801 atgctgttgc ccacttcaca aatcaacacg ccatcatcag caaggtaatc tgccgcgtta
    58861 ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag atgccaggcc cagttccggc
    58921 tcgtgacggt attcgtttgg caggtcggac atatcttccg cgtcgacata cggcgggtta
    58981 gtgacaatca gatcgtattg cactttcggc aaatcacgga acagatcgga acgaatcgga
    59041 atgacgttgt ggatcagacc gtgttcttcg atgttctgtt cggcaaccgc cagcgcgtcg
    59101 ggagagatat ctaccgcgtc gacttccgct tccgggaagg cataagcaca ggcaatggcg
    59161 atgcagccgc taccggtaca catatctaaa atatgctgcg gttgcttgct gatgagtccg
    59221 gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg gcaccagcac gcgttcatcg
    59281 acgtaaaatt catgaccgca gaaccaggct ttgttggtca ggtaagccac cggaatgcgc
    59341 tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt tttcgctgga ggtcagacgc
    59401 gcggtgcgca tatcttccgg gatatccagc ggcaggtaga gcgatggtaa caccagctgt
    59461 acggcttcat cccacgggtt atcggtgccg tgaccgtacc agatatttgc cgcgctgaag
    59521 cggcttaccg accagcgcaa tatgtcctga atggtttgca gctcatttac tgcttcatca
    59581 acgaaaattt tatccacgta ttcctccagg gcatgatcgc aataatttcg gcggctagtt
    59641 tgccatgaag atgacgataa atcagcattc acgcgcggtg agtgaggaaa aatacgttta
    59701 aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa aattagaaat agagacagat
    59761 aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc cagttgatgg
    59821 cggggactcg caagattaag caggacacga ttgtccaccg accgcagcgt aaaaaaatca
    59881 gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga tgccagccat tatttctccg
    59941 atgagtttca gccgttatta aataccgaag gtccggtgaa atatgttcgc ccggatgtca
    60001 gccattttga ggcgaagaaa ctgcgccgcg gcgattattc accggaattg tttttggatt
    60061 tacacggtct gacgcagctg caggccaagc aggaactggg cgcgttgatt gccgcctgtc
    60121 gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca cgggaagcat attttgaagc
    60181 aacaaacacc gctgtggctg gcacaacatc cgcatgtgat ggcatttcac caggcaccga
    60241 aagagtacgg cggtgatgcg gctttgttgg tgttgattga agtggaagag tggttgccgc
    60301 cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc gtaaacgcct tatccggcct
    60361 acaaatatgt gataattcaa taaattgcaa ttcacttcgt aggcctgata agacgcatca
    60421 gagcaatact acgaacttca gttcattcaa tacatactga aaacgacacg tcgaatcaat
    60481 cggttgccca ctactggtat tcacattcac cacaatatcg tgtagtactt tatccgttac
    60541 cggatgacga gttggcgtaa ccgttaccgt tgatttatca caaccgtaag aggtaaaggt
    60601 taacaattct gataccgcgc ggcagtatgc tcctctgcca tcattatcag aaaaattact
    60661 gccatcacag agtctgccgc cttccatcag gtaggtcgaa agcgtggtgg tcactgttga
    60721 ccccgtggac tgaattcgta acgccttatt atggctgaca taattttcac tccaggagat
    60781 atcgtatgaa gcgaccggta tcgttgggca tattgtgtta tgcactgtag gggccgtata
    60841 gcttccaaat ccgggtaaat ctgtatagga ataaaaggat gcgatccaag ggcaaccata
    60901 atcatcagat gcaacggtaa ttttaaacgt catatatgca caacgaaaaa ttttcgtatc
    60961 tatgccaggg caatttgagt tattaattgt atcaaagtca gtcagtacca aagttggagc
    61021 tgaacccgac ccagaccagg aaataacccg caacgtatcg cctttatacg caaaagaatc
    61081 atatttccca tatgcaacct cctttggcgt tatcaccgta acattgaaaa cactgctcgc
    61141 tacagcaccg gttaccttgt ccatggcata ataaactctc acactgtcaa aactcgcact
    61201 cataagttta tctccagcat aactcttaga tgcgccgaca acactcagaa cgagcagcat
    61261 taggctcgcg aaaattatgg tccatttttt catttcagaa atactcaaag gttgccgtca
    61321 ctgtggcact aaagtctccg gacgttacat ctcgcccgct ctttgcctgt aaccaggtat
    61381 taaaatttag gctgttattt ccctgattca gcgggagcgt gacgcctgtt gtattgttga
    61441 ttggcaccga tgtgcctgcc gcagtttcaa taccaatccc agcgccggat gctgatgacg
    61501 aggagtcaaa ggccagaaag ccgggtagag cactatcttc tgttcctgtc aacgttactt
    61561 tgacattaaa caatgtagaa ctatgacaat ctttcaattg aaacaccaat ggcactcgct
    61621 ctgactgccc aaggttcatt aaatcactgg cggcaatggc aggaaaatgt acttctgcca
    61681 actggctacc attaatcact ggcgtacacg atttactgat taaattgccg gtaaagtgca
    61741 aattatctcc tgctattgtg gttccactgc ccagcaacat caggctacta ataaaaaaca
    61801 acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg gcccaggcct caaagtccgc
    61861 ctctggcaga atgacaccac tcttttttac tggcacagcc gttaacaccg gtaaatcggt
    61921 ttcattcacc acaagtggcg tattgatcgt aaaactctgc cctgcctgtt tgagctgaat
    61981 gcctaaacca gtgatattgc tgcttactgc agcattatca aaatcgcttg gtgtgccact
    62041 ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc gtatcgcagg taatggaata
    62101 cggcacggtt tgctcataat tgctgccatt aatgtcatca ataagcacat cctgaaaagt
    62161 tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc ggctgcacta aataaccgtg
    62221 aaaagagacg tttgagagtg ccatcgagca aaagccccac agcaaaataa gcgagatccg
    62281 tttcattggt aatccaccac aattgtggca ctggcgttaa attcagctgc cgctaacgtt
    62341 gtaccactct gtttcaccgg cacagcttcc agaacgggaa ctcccgagga aaaattaaat
    62401 ggcaaccatg agccagaggt taagtcgagt attgtgtgat cactactttt ctgaacccga
    62461 ataccaaacc caggaatacc cgtgctgata actgtttcac cgttaatcac gaccgttgtc
    62521 gcctgcatct gtagacgcag gtcatccatt gccagattgt tacaaacaag cgagtagtca
    62581 atgggacgtt tataatcaac cctattgatc ttattaatga atacattacc aaactcaact
    62641 gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa gcacccgtaa attcatactt
    62701 actgacttac tcgctgcaac tgcaaagccg ctaccaacac aacatatcag taacccagtt
    62761 accgcccaga cagaacaaga tcctttcata ctggctctcc cggcctcagc ctttgcggct
    62821 ctgctcttca tcgacctagc aactgttacc gttgcaagtg aaaaagagcg gcatccgagc
    62881 gccatagtcg ttgacatagg tcagtaccgg cacgctaccc atcttcacat tcagcggtgc
    62941 agaggttttc ggcgacatca ctattggcga gaaaccggct gccggattgc catttttctg
    63001 tgtgctggca ttactgaaaa tgacgtaata cgacgtcggg ttattgacgg taaattcctg
    63061 accgttgcgg gttaaagtaa tcttatattg ctaggggttt ttcatgctga cgttctccag
    63121 cgccttcggt cgccagaaca acttaatacg cgtttgtagt gctatttgca gcgtgttggc
    63181 tttgtttgat tttggcggga tctcacgcac gttgaagtag aacaggcttt cacgatcggc
    63241 aggcaatttg ttgatatccg gcattccctg cacttttacc tgcccgttca tcattgaatc
    63301 gatgcgttgc accggtggca gaacagttaa cggtgaactg atcttgttac ccttttcatc
    63361 ttcgatccag ctttgggcca gataaggcag tttcggatcg ttattgcgca acgtcacgct
    63421 gattgatttg tcgctttcat taaatatcaa acgggtacga tctggcgtta cagaagcctg
    63481 ggcagacaaa ctggcagcgc taagcagtaa cgctagcgtc gtagcaccca atttggcaga
    63541 gcaaagtaaa tcagacatgg atcattcctt aatcacgtta ttggttagct gaaacagaaa
    63601 cagggggatt ggtgggtgtc acctgttgcg tctgctcctg aatgacaggc tttatgtcat
    63661 caggaacaac cggcgcgacg ttgcctttat gctggcatgg cagtaacaag ccgttaaaca
    63721 gatcggcagg cagcgggtcc ggcaggttga tatcgcaatg cgcaacacca ctccagaaca
    63781 cactcatgtg ttcgccaggt ttcactccag ccagataaac attgccgtca tcatcgacaa
    63841 ggcccactgt ctgctcgtta tcatttttta cttctgcgcc aaacggagga tggctgccgt
    63901 cttgtaaacg cagcaccgcc atcgcttttt gaccactgat gacggcaaat ttgcggtagc
    63961 caatcgcccc ttcagttagc gtggcttgta ccaccgactg ggttgcttcg gcgttttccg
    64021 gcagtttgtt gaggtcgata tacgcctgat tgcggtaata gttattgacg tcagaaacga
    64081 cggctttacc aaacatattg gtataaacag cagcaccgtt accttctacc ggaacatcgg
    64141 caacgccatc ggcatcaatc aacaagcgtg tcccgcccat attctgggta cggtgaagtg
    64201 cgccaccgtg ggcagtaagc gttgcgccgc cctgtaacga caagcccgca gaggtgtatt
    64261 gcccttcatg gtagttcgca ctgaggtcaa cctgcgccag cgaaccatca tggctgtaat
    64321 agccgtcaac gctggtgtgt ttgtcactgg tgccgacgtt caactgatag tgagtcgcgt
    64381 catcgacacg gctgaaataa ccgacctgac tgctgtccgt cccactgcca tagtaccgtt
    64441 atagctaacg gtactgttgt cgccccacgg catactgagc gaaatgtaca tgcctttgtc
    64501 ggcctggttg tcatactcgt aacggtagcc agtcatcgag atgctgacat tacgaatact
    64561 gcccatattg aaatagtgcg agagcatgat gttgtagttt gtctgctcct cgcgatccca
    64621 gtaggtatgg cgggtatagt tgagataaac cgaaacaccc gcatcgcgga agttctggtt
    64681 ataagtggcg gtgtacatct ctttgtcgtt gcccgtgcgt accattccgc tgtcactggc
    64741 atccagatac tcgctcatgg tcataaagtt ctcttccgag aagcgatatc cagcaaaagt
    64801 aacgcggctg ttgagctggt caaagtcttt ggaataactc acacggaagg agttaccgtc
    64861 cagcgaacct ttgccataag cggtgtcttt atccagtttg gtgtgcgagt gagtaacatc
    64921 aaacgcgacc gcgccgaatg tagacaaatc gcgaccgaca ccaagcgccg cagactgata
    64981 gttttcatct cccagtgcgc cgccatataa cgaccagccg ttagcgatcc cccaggaagc
    65041 ttcagcacca gaaaaaaatt caccctcgac atggtgtccc cactcttgcg gacggcccat
    65101 catgatctta tagcgaacct gacctggacg agtgaggtat ggcatcgagg cggtgctgat
    65161 gtcatattcc tgcacctggc cgttctgttc ttcaatgcga atatgcaacg taccggagac
    65221 agaatcacca agatcctgaa tacgaaacgg tccagccgga acctgcgttt cgtaaatcac
    65281 acgccccatc tggctgacgg tcacttttgc tgtggtgtgt gccacgccgg aaatgtctgg
    65341 cgcgtagccg cgcagattgg gaggcaacat ttgatcgtca gtactgacac tgccaccaac
    65401 atagttaaaa ccatcaaaaa tatcggatct gaggtaatcc tcgcccagcg ccagtttggc
    65461 ttttaatgat ggtaacgccc gcctggcata gtagcgactc cactcccatt tttttgagtt
    65521 tcatcgccgc tgaattcatc gtcatcatta ctgcgagtat gttgatagtt agtctgccag
    65581 tcagcacgca tacgccacgg ccctaggtta accccaaccg tcccgttgcc gctgatctcg
    65641 ttactatcat caccgccatt ttcttcgtgc cgggtttgtg cgttgatgct gtagtccgcg
    65701 acgatcccgg agatgccgtc atcccaacgt gaaggcggat ccctgtcggg ataagtatat
    65761 tcgaggtaag cctgtggcag tgaaatgact aatgcggact ggcttaaatc agccttaatt
    65821 tccatgcctt ccagttgacc ggatttcagg catttagcat cgtggctcca ttgcagattt
    65881 ttcgccacgt cttcttttaa accaaactgc gctaccagtt ccggtgtcag acaagcatag
    65941 cttttgctcg cgtcatcttc accagcatac cagtaaatat cgtactcttc cgccaatggc
    66001 tgtttattta gttgaacctg taaattgtat tttccgggct caacatatcc ctggctggaa
    66061 aaacgcttca tatcaatttt tgtgtcgcct tttaattcca gaaaacggga atcaaactga
    66121 atgtcgtctt cagcccagac actgctataa ctccctgaca ttgccagcgc aatgcaccac
    66181 ggaagaatcc gtaatcgaaa aagagaatgg tcaggcatac acgtttctat ccgtttttat
    66241 tcttgttcgg tcaaaataca tttaaatccc ccatagcagt gtcgctatgg gggtatgcct
    66301 tacaggtaag taatctggaa ggtggtattt gcttcaaaat tacccagatc tggcgcatca
    66361 gctgcgccga ccagccaggc gttaaagttc agtgtctgct tcgctttgcc tttgttggta
    66421 gaagtgtctt tcactatttt ctggtcaata cccatgccgc ttttgtaaga ggtacccaga
    66481 gagtcaccga tcgccaggct aacattgtta aatgccgcac cggtatcggt attgaaaatg
    66541 gtgtaataat tgccgctatt tgcagaagaa acggtaccag taaaggtagt ggtcattgtt
    66601 tcctgagtat caaatacgca atcttgcaga cgaatctgga aacttttcgg cgtggtggtg
    66661 ccgttatttt tcagtttttc ggcaccgata tcacccatat caacttccag tttgtgatcg
    66721 tccggaacga tagaacacac tgagtcttca atggtgccgt aaaaacgtgc agtaccgttg
    66781 ttacccgcag cgattgttgc cgtagaggtg aacacgccca tcaccattgc cgcagcaata
    66841 gctgttt
//
LOCUS       DAJTYH010000007        66483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTYH010000007 DAJTYH010000000
VERSION     DAJTYH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..2433)
                     /gene="glgP"
                     /locus_tag="OO609_000456"
     CDS             complement(<1..2433)
                     /gene="glgP"
                     /locus_tag="OO609_000456"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6631996.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHID"
     gene            complement(2452..3885)
                     /gene="glgA"
                     /locus_tag="OO609_000457"
     CDS             complement(2452..3885)
                     /gene="glgA"
                     /locus_tag="OO609_000457"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6631997.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3885..5180)
                     /gene="glgC"
                     /locus_tag="OO609_000458"
     CDS             complement(3885..5180)
                     /gene="glgC"
                     /locus_tag="OO609_000458"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6631998.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5198..7171)
                     /gene="glgX"
                     /locus_tag="OO609_000459"
     CDS             complement(5198..7171)
                     /gene="glgX"
                     /locus_tag="OO609_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6631999.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7168..9354)
                     /gene="glgB"
                     /locus_tag="OO609_000460"
     CDS             complement(7168..9354)
                     /gene="glgB"
                     /locus_tag="OO609_000460"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6632000.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9627..10730)
                     /gene="asd"
                     /locus_tag="OO609_000461"
     CDS             complement(9627..10730)
                     /gene="asd"
                     /locus_tag="OO609_000461"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6632001.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10922..11515
                     /gene="yhgN"
                     /locus_tag="OO609_000462"
     CDS             10922..11515
                     /gene="yhgN"
                     /locus_tag="OO609_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6632002.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11572..12912)
                     /gene="gntU"
                     /locus_tag="OO609_000463"
     CDS             complement(11572..12912)
                     /gene="gntU"
                     /locus_tag="OO609_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6632003.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12916..13443)
                     /gene="gntK"
                     /locus_tag="OO609_000464"
     CDS             complement(12916..13443)
                     /gene="gntK"
                     /locus_tag="OO609_000464"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6632004.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13582..14577)
                     /gene="gntR"
                     /locus_tag="OO609_000465"
     CDS             complement(13582..14577)
                     /gene="gntR"
                     /locus_tag="OO609_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6632005.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14801..15496)
                     /gene="yhhW"
                     /locus_tag="OO609_000466"
     CDS             complement(14801..15496)
                     /gene="yhhW"
                     /locus_tag="OO609_000466"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6632006.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15619..16656)
                     /locus_tag="OO609_000467"
     CDS             complement(15619..16656)
                     /locus_tag="OO609_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6632007.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16989..17477
                     /gene="yhhY"
                     /locus_tag="OO609_000468"
     CDS             16989..17477
                     /gene="yhhY"
                     /locus_tag="OO609_000468"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6632008.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17536..19278)
                     /gene="ggt"
                     /locus_tag="OO609_000469"
     CDS             complement(17536..19278)
                     /gene="ggt"
                     /locus_tag="OO609_000469"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6632009.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19398..19838
                     /locus_tag="OO609_000470"
     CDS             19398..19838
                     /locus_tag="OO609_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6632010.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19825..20568)
                     /gene="ugpQ"
                     /locus_tag="OO609_000471"
     CDS             complement(19825..20568)
                     /gene="ugpQ"
                     /locus_tag="OO609_000471"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6632011.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20565..21555)
                     /locus_tag="OO609_000472"
                     /pseudo
     CDS             complement(20565..21555)
                     /locus_tag="OO609_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21557..22402)
                     /gene="ugpE"
                     /locus_tag="OO609_000473"
     CDS             complement(21557..22402)
                     /gene="ugpE"
                     /locus_tag="OO609_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6632012.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22399..23286)
                     /gene="ugpA"
                     /locus_tag="OO609_000474"
     CDS             complement(22399..23286)
                     /gene="ugpA"
                     /locus_tag="OO609_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6632013.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23384..24700)
                     /gene="ugpB"
                     /locus_tag="OO609_000475"
     CDS             complement(23384..24700)
                     /gene="ugpB"
                     /locus_tag="OO609_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6632014.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25097..25810)
                     /gene="livF"
                     /locus_tag="OO609_000476"
     CDS             complement(25097..25810)
                     /gene="livF"
                     /locus_tag="OO609_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6632015.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25812..26579)
                     /gene="livG"
                     /locus_tag="OO609_000477"
     CDS             complement(25812..26579)
                     /gene="livG"
                     /locus_tag="OO609_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6632016.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26576..27853)
                     /gene="livM"
                     /locus_tag="OO609_000478"
     CDS             complement(26576..27853)
                     /gene="livM"
                     /locus_tag="OO609_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6632017.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27850..28776)
                     /gene="livH"
                     /locus_tag="OO609_000479"
     CDS             complement(27850..28776)
                     /gene="livH"
                     /locus_tag="OO609_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6632018.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28824..29933)
                     /gene="livK"
                     /locus_tag="OO609_000480"
     CDS             complement(28824..29933)
                     /gene="livK"
                     /locus_tag="OO609_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6632019.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30357..30740
                     /gene="panM"
                     /locus_tag="OO609_000481"
     CDS             30357..30740
                     /gene="panM"
                     /locus_tag="OO609_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6632020.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30939..32042)
                     /gene="livJ"
                     /locus_tag="OO609_000482"
     CDS             complement(30939..32042)
                     /gene="livJ"
                     /locus_tag="OO609_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6632021.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32313..33167)
                     /gene="rpoH"
                     /locus_tag="OO609_000483"
     CDS             complement(32313..33167)
                     /gene="rpoH"
                     /locus_tag="OO609_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6632022.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33412..34470)
                     /gene="ftsX"
                     /locus_tag="OO609_000484"
     CDS             complement(33412..34470)
                     /gene="ftsX"
                     /locus_tag="OO609_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6632023.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34463..35131)
                     /gene="ftsE"
                     /locus_tag="OO609_000485"
     CDS             complement(34463..35131)
                     /gene="ftsE"
                     /locus_tag="OO609_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6632024.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35134..36633)
                     /gene="ftsY"
                     /locus_tag="OO609_000486"
     CDS             complement(35134..36633)
                     /gene="ftsY"
                     /locus_tag="OO609_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6632025.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEEAAKEEITDEELEAQA
                     LAAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFIS
                     LFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEE
                     MGEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTF
                     RAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNK
                     SHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKL
                     DGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36783..37379
                     /gene="rsmD"
                     /locus_tag="OO609_000487"
     CDS             36783..37379
                     /gene="rsmD"
                     /locus_tag="OO609_000487"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6632026.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37369..37638
                     /locus_tag="OO609_000488"
     CDS             37369..37638
                     /locus_tag="OO609_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6632027.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37641..38000)
                     /locus_tag="OO609_000489"
     CDS             complement(37641..38000)
                     /locus_tag="OO609_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6632028.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38141..38767
                     /locus_tag="OO609_000490"
     CDS             38141..38767
                     /locus_tag="OO609_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6632029.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38841..41039
                     /gene="zntA"
                     /locus_tag="OO609_000491"
     CDS             38841..41039
                     /gene="zntA"
                     /locus_tag="OO609_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6632030.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41141..41386)
                     /gene="tusA"
                     /locus_tag="OO609_000492"
     CDS             complement(41141..41386)
                     /gene="tusA"
                     /locus_tag="OO609_000492"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6632031.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41607..42272
                     /locus_tag="OO609_000493"
     CDS             41607..42272
                     /locus_tag="OO609_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6632032.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42345..42902
                     /gene="dcrB"
                     /locus_tag="OO609_000494"
     CDS             42345..42902
                     /gene="dcrB"
                     /locus_tag="OO609_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6632033.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42906..44123)
                     /locus_tag="OO609_000495"
     CDS             complement(42906..44123)
                     /locus_tag="OO609_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6632034.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44257..45306
                     /locus_tag="OO609_000496"
     CDS             44257..45306
                     /locus_tag="OO609_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6632035.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45361..45948
                     /gene="acpT"
                     /locus_tag="OO609_000497"
     CDS             45361..45948
                     /gene="acpT"
                     /locus_tag="OO609_000497"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6632036.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46059..47633
                     /gene="nikA"
                     /locus_tag="OO609_000498"
     CDS             46059..47633
                     /gene="nikA"
                     /locus_tag="OO609_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6632037.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47633..48577
                     /gene="nikB"
                     /locus_tag="OO609_000499"
     CDS             47633..48577
                     /gene="nikB"
                     /locus_tag="OO609_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6632038.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48574..49407
                     /gene="nikC"
                     /locus_tag="OO609_000500"
     CDS             48574..49407
                     /gene="nikC"
                     /locus_tag="OO609_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6632039.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49407..50171
                     /gene="nikD"
                     /locus_tag="OO609_000501"
     CDS             49407..50171
                     /gene="nikD"
                     /locus_tag="OO609_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6632040.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50168..50974
                     /gene="nikE"
                     /locus_tag="OO609_000502"
     CDS             50168..50974
                     /gene="nikE"
                     /locus_tag="OO609_000502"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6632041.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50980..51381
                     /gene="nikR"
                     /locus_tag="OO609_000503"
     CDS             50980..51381
                     /gene="nikR"
                     /locus_tag="OO609_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6632042.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51501..51860)
                     /locus_tag="OO609_000504"
     CDS             complement(51501..51860)
                     /locus_tag="OO609_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6632043.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51857..52132)
                     /locus_tag="OO609_000505"
     CDS             complement(51857..52132)
                     /locus_tag="OO609_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6632044.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52191..53315)
                     /locus_tag="OO609_000506"
     CDS             complement(52191..53315)
                     /locus_tag="OO609_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6632045.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53315..56050)
                     /gene="rbbA"
                     /locus_tag="OO609_000507"
     CDS             complement(53315..56050)
                     /gene="rbbA"
                     /locus_tag="OO609_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6632046.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56047..57114)
                     /locus_tag="OO609_000508"
     CDS             complement(56047..57114)
                     /locus_tag="OO609_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6632047.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57480..59102)
                     /locus_tag="OO609_000509"
     CDS             complement(57480..59102)
                     /locus_tag="OO609_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6632048.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59364..60953)
                     /locus_tag="OO609_000510"
     CDS             complement(59364..60953)
                     /locus_tag="OO609_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6632049.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61337..62368
                     /locus_tag="OO609_000511"
                     /pseudo
     CDS             61337..62368
                     /locus_tag="OO609_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62455..62931)
                     /locus_tag="OO609_000512"
     CDS             complement(62455..62931)
                     /locus_tag="OO609_000512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632050.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62924..63688)
                     /locus_tag="OO609_000513"
     CDS             complement(62924..63688)
                     /locus_tag="OO609_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6632051.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63934..64695
                     /locus_tag="OO609_000514"
                     /pseudo
     CDS             63934..64695
                     /locus_tag="OO609_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64802..65302)
                     /locus_tag="OO609_000515"
     CDS             complement(64802..65302)
                     /locus_tag="OO609_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632052.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65453..>66483)
                     /locus_tag="OO609_000516"
     CDS             complement(65453..>66483)
                     /locus_tag="OO609_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6632053.1"
                     /translation="LSQPLGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFS
                     INWAKNDLLTSHKWKVDRKLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSG
                     QNSGGVWWDVATNITNAHQSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLS
                     GGILITRDGLTFGQNVDGTLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDD
                     VILDEKTIGKNYDLPQTSQLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAV
                     ISYSKDTENMAGIVGEDGIAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGL
                     YQIAATCL"
BASE COUNT        16156 a        17850 c        16317 g        16160 t
ORIGIN      
        1 atcgatatgc cagatatgat cagcgtactc tttgatagta cggtcagaag agaagtagcc
       61 catattggca atgttcagca tcgcttttgc ggtccactct tcctgaagct cgtagagttc
      121 gtcgacttta tcctgacaat cgacatagct gcgataatcc gccagtacct ggtagtgatc
      181 gccgaagttg atcagcgaat caaccagatc gcgatagcga cccggatctt ccggactgaa
      241 tacaccgctg ccgatttgcg tcagcacctg atgcagctcc tcatctttct cgtagtattc
      301 acgcggtttg tagccctgac gacgcagttc ttccacttct tccgctgtgt taccaaaaat
      361 aaagatattg tcagcaccga catgatccag catctcgaca ttcgcaccgt ccaacgtacc
      421 gatagtcagc gcaccgttaa gcgcaaactt catgttactg gtgccggaag cttccgtccc
      481 tgccagcgaa atctgttcag acagatctgc cgccggaatg atcaactgcg ccaggctaac
      541 gctgtagttc gggatgaaca cgactttcag cttatcgcca atctgcggat cgttgttgat
      601 cactttcgct acgtcattga tcaaatgaat aatgtgcttc gccatgtaat aggccgaagc
      661 cgccttaccg ccaaaaatat tcacgcgcgg tacccacttc gcatccgggt cggccttgat
      721 gcggttatag cgggtaatca catgcaacac attcatcaat tgacgtttgt attcgtgaat
      781 acgtttgatt tgtacatcga acaacgcctt tggattcacc accacattca gctgctgggc
      841 gatatactct gccagacgct ttttgttctc cagcttcgcc tgatgcacag cgtgattaac
      901 cattgggaaa tcacagtgtt gttgcagctc attaagcagg ctaaggtcgg tgcgccagtt
      961 acggcccagg tgttcgtcca gcacggctga aagcgatggg ttcgctaccg ccagccagcg
     1021 acgcggcgtc acaccgttgg tgacgttggt gaaacgaccc gggaagattt tcgcaaagtc
     1081 ggcaaacaac gattgcacca tcagattaga gtgcagttcc gatacaccgt taactttgtg
     1141 gctcacaaca accgccagcc aggccatacg cacacgacga ccgttggatt catcaatgat
     1201 cgacgcccgt cccagcagat cggtatcgtt cggatactgt tcctgcaagg ttttcaggaa
     1261 atagtcgttg atttcaaaga tgatctgcag gtgacgcggc agaattttac ccagcatatc
     1321 aaccggccag gtttccagcg cctcgctcat cagcgtgtgg ttagtgtagg agaagacctg
     1381 acaacacacc tcaaacgcgt cgtcccagct aaattggtgc tcatcgatca gcagacgcat
     1441 catctcagga atcgacagta ccggatgggt atcattgaga tgaatcgcga ttttatccgc
     1501 caggttatcg taggttttat gcaactgata atgacggctt aaaatgtcct gaatggtcga
     1561 ggaaaccaag aagtattcct gacgcaggcg cagctcacgc ccggagtagg tggagtcatc
     1621 cggatacagt acgcgagata cgttctcgga gtggttttta tcttccactg ccgcgaagta
     1681 gtcaccctgg ttgaatttac cgaggttaat ttcgctactg gcttgcgcac tccacaaacg
     1741 cagcgtgttg gtcgcgtcgg tgtcgtaacc agggattatc tgatcgtaag cgactcccag
     1801 aatctcttcg gtttcaatcc agcgcgtttt tttaccttcc tgctgaatgc gaccaccaaa
     1861 acggacttta tagcgcgtgt tgtggcgttt gaattcccac gggttaccgt attccagcca
     1921 gtagtctggc gactctttct ggctaccgtt aacgatgttc tgcttgaaca taccgtagtc
     1981 atagcggatg ccgtaaccgc gccccggcaa ccctaacgtc gccagagaat caaggaagca
     2041 agccgccaga cgtcccaggc caccgttacc gaggcctggg tcattttctt catcaatcag
     2101 ctcttcgaga tttaacccca tcgcttccag tgcgccctgt acatcttcgt aaattcctag
     2161 cgacaacatg gcgttggaga gcgtacggcc aatcaaaaac tccatcgaca ggtagtaaac
     2221 ctgacgagtt tcttgcgaca actgggcacg gtttgaacgt aaccagcgct ccacgagacg
     2281 atcgcgcaca gcaaataacg ttgcgttcag ccattcatgt ttattggcga cgaccgggtc
     2341 ctttccaatc gtaaacatca gcttgtaagc gatagagtgc ttaagagctt ctacgctaag
     2401 cgtgggcgat gaatatgtaa acggagcatt catataggcg tttcctgaaa actatttcaa
     2461 gcgatagtaa agctcacggt acgacttcgc cgcgacctgc cagctaaaat ccattgccat
     2521 agcctgacgt tgcacaaacc gccacagtga aggacgggac cacagtacaa aagcacgccg
     2581 aatagcccgt aacagcgacc aggcattact atcttcaaag acaaacccac tggcgacgcc
     2641 atctgcaagg ttctcaagag aacagtcaga aaccgtatca gcaagcccac cggtgcgccg
     2701 cactaacggc agcgtaccgt acttcaatcc ataaagttgc gttaagccgc acggttcaaa
     2761 acggctgggc accagaatga cgtccgcgcc gcccataatg cgatgcgaaa atgcttcgtg
     2821 atagccaatc tgaacgccca cctgaccggg gtattccgct gccgccgcaa ggaaaccttc
     2881 ctgcagcacc ggatcgcccg cgccgagtag cgccagctgc ccgccctgct ccagaagccc
     2941 cggtaaggct tccagcacca ggtcgagacc tttctggctg gtcagacggc tcaccactgc
     3001 aaaaagcggc actttatcgt caaccttaag ccccattgcg atttgtaact ggctcttatt
     3061 ttccgcttta tcttccaacg tatcgcgggt gtaacgcgag gccaacagta agtccgtctc
     3121 tggactccag attttctcgt ccacgccgtt cagtacgcct gaaagacgcc cttcacggtg
     3181 acgctgttgc aacagacctt ccataccgta ggcaaactgc ggttcggtga tctcgcgagc
     3241 gtaggttgga ctgaccgccg taatgtgatc ggcatagtac agaccggcct tcaggaaaga
     3301 gatttgtccg ttgaattcca gcccatgaat attaaagaat gaccatggca attggatgtc
     3361 attcatgtga tgtgcataaa acatgccttg ataggccagg ttgtgcacag taaacaccga
     3421 cttcgcagga cgcccgcgcg ccgccagata cgcaggcgca agacctgcat gccagtcgtg
     3481 cacatgcacc acatcaggac gccagaatgg gtcaagcccg ctggccattt ctgccccaac
     3541 ccaccccagc agcgcaaaac gcaatacgtt gtcggtatag gcaaataagt tggtatcgtg
     3601 atacgggctt cccggacgat catagagatg cggcgcgtca atcaggtata tgccaacccc
     3661 gttgtaatga ccgaacaaca gcgtgatatg cccggcgaaa gtatcacgac gggatactac
     3721 ctgcgcatcg gtcacgccac ggcgaatatc gggaaatgca ggcaacagta cgcgagcgtc
     3781 aacgccgtct gcgatttgtg ctgcgggtaa tgccccaata acatcagcca gaccgccggt
     3841 tttaagcagc gggaacatct ctgaacatac atgtaaaacc tgcattatcg ctcctgttta
     3901 tgccctaact tccgtagcat ttcgcgcgtt accagcacga tgccttcttc tgaacgatag
     3961 aaacgacgtg catcttcctc tgcgttttca ccaatcacca tgccttccgg gataacacaa
     4021 gcacgatcga tgacgcagcg gcgcagacgg cacgagcgac ctacccatac ttccggtaac
     4081 aatacggcgg aatcaatgtt gcagaatgaa ttcacgcgaa cgcgcgagaa cagaacggac
     4141 tgcaccacca ccgaaccgga gatcacacaa ccgccggata ccagtgagtt aagggtcatc
     4201 ccgtggctac cggagcgatc ctgcacgaat ttcgctggcg gtaatgattc attgtaggtg
     4261 cgaattggcc aattgcgatc gtacatatcc agttccggca ccacagaggc cagatcgaga
     4321 ttcgctttcc agtaagcttc cagcgtaccc acatcgcgcc agtacggctc ggcatccggg
     4381 tcggattgta cgcaagagag cgggaacggg tgcgcatagg ccagaccggc ttcggtgatc
     4441 ttgggaatca aatctttgcc aaagtcgtgg ctggagttct catcacgatc gtcttcttcc
     4501 agcagttcat acagatagtc ggcgtcaaag acgtagatac ccatactcgc cagagatttg
     4561 ctcggatcgt tcggcattga cggtgggtta gcaggttttt caacgaattc gataatttta
     4621 tcgttctcat caaccgccat aacgccaaat gcggaggctt cttcaatcgg tactggcata
     4681 caagcaacgg tgcaacgtgc gcctttttcg acgtgatcga taagcatacg cgagtagtct
     4741 tgcttgtaga tatggtcgcc cgccaggatc accacgtatt ccgctttata acggcggata
     4801 atgtcgaggt tttgggtgac cgcatctgcg gtgccgcgat accagttttc ccctttcatt
     4861 ctctgctgtg ctggcagcag atcgacaaac tcgttcattt cttcattgaa gaatgaccag
     4921 ccgcgctgaa tgtgctgcac cagagtgtgg gactggtact gggtgatcac gcccatacga
     4981 cggatcccag agttgatgca gttagacagc gcaaagtcaa taatgcggaa cttaccgccg
     5041 aagtgtacgg ccggttttgc tcgcttattg gttaaatcct tcaggcgggt accacgtcct
     5101 cccgccagta tcagggcaac agatttcaat ggcagctggc gcgccaacat taagtgatcg
     5161 ttcttctcta aactaaccat gactaactcc ttttttatca tctctggaac acacacaatc
     5221 cgtgtgcagg tccctgccag acaaccgtaa tcactgggtt atcctctcca gcgaatgggg
     5281 gaatggcgtg ccactcccca gcaggtaaaa caatctctgt tacctcaagc gtggcgttaa
     5341 ttgcgatcaa aaagcgatcc gagagcagaa tttgcagctg tttcggcccg ttttgccact
     5401 catccgtgct taaaggttga gcatatcgat ttagccaacg gacattgccg tcaccttctt
     5461 cccaccagcg attctccacc aaagcgggaa tgcgcttgcg cagatggatt aacgcggcgg
     5521 taaatgcggt taaaccactg cttgcctgcg accagtccaa ccagtttaat tggttatcct
     5581 gacagtaggc attgttattg ccatgctggc tgtgaccatg ttcgtcaccg gccagtaaca
     5641 tcggcgtgcc ctgggagagc aacaacgttg ttaacagggc atgaatgctg tcgcgccgcc
     5701 gttcaaccag gtcaagagta ccgcctaacc cttctttacc atgattgtta ctgtaattgt
     5761 tgttggtccc gtcgcgattt tcttctccgt ttgcttcatt gtgtttatgg ttgaagcaaa
     5821 cgcagtcgcg aagcgtaaaa ccgtcatgcg cggtgacgag attaatcgcg gcactcggca
     5881 gacgaccatt acgtttaaaa acatcgctgg aggcagcaaa acgcccggca aacgccccca
     5941 gaggcaaatc ataatgtagc cagaaacgac gggcagcatc gcggaaatga tcgttccact
     6001 cggcaaacag cggcgggaaa tttcccacct gataaccact aggagcgata tcccacggtt
     6061 cagcaattaa cttcacctgc gagagcaccg ggcagttctg gatagcggta aacaacggcg
     6121 catcctgacg gaactctggc gtacggccca tgactgccgc cagatcaaag cggaaaccat
     6181 cgacgtggca ggtttctacc caataacgca ggcaggcgct ggcataatcc accaccgccg
     6241 gatgactcaa attgagcgtg ttgccgcaac cggtccagtt gtgataatcg ccgtcttctc
     6301 ttatccaata atagctacgg ttatcgatcc cacgcagcga gaataacggg ccgtcgaggt
     6361 ccagttccgc actatggttg agcacgatat caagaatgac ttcgataccc gctttatgca
     6421 gtgctttgat tgcatcgcga aactcatcca gcgccgtttc tggcgagcag gcatacgccg
     6481 gatgcagcgc aaacatcgcc accgggttgt aaccccagta gttacttagc cccatgcgtt
     6541 gcagacgtgg ttcactggca aactgcgcca ctggcagcag ttccagcgcg gtaatgccca
     6601 attgtttcaa atagttgatc atcaccggat gcccgagggc tttataagtg ccacggatct
     6661 cgaccgggat ctccgggtgc aagtacgtta atcctttgac atgggcttca taaatgatgg
     6721 tgctgcccca cggcgtgcgc ggcggggcgt catcttccca gtcatagtga tcaaccacca
     6781 ctacgcattt cggcgcaatg gcggcgttgt cgcgatagtc aggttcatta tgaccggcgt
     6841 gcagcagcgg gttatcttta aactccccgt caatttgccg cgcgcaagga tcaatcaaca
     6901 acttcgccgg gttaaagcga tgcccctcgg cgggttgcca ggggccatga acgcgataac
     6961 cataacgcaa acccgggcgc gcatccggca gataaccgtg ccaaatgtcg ccactgttcc
     7021 ctggcaagtc atagcgatgt tcctggccat tggcgtcaaa gacacacagt tctacccgct
     7081 cggcatgagc ggagaaaagt gtgaagttga cgccctgacc gtcgtaatgc gcgccgaggg
     7141 gagcgggttt gccaatggcg agttgtgtca ttctgcctcc cgaaccagcc agatagtggc
     7201 cagcggtggt agcgtcaggc ttagtgaatg ctgacgaccg tggctggcaa tctcatcgct
     7261 gtgtaccgtg ccgccattgc ctgcattact gccgtgatag tgtatggaat cggtattgag
     7321 gatttcacgc catttacctg gctgattaat gccgaagcga taatcatgac gcggcaccgg
     7381 cgtaaagtta ctggcaacga taatttcgtt accctcttta tcgcgacgca caaagatcag
     7441 caccgagcgt tctttgtcat ccaccaccag ccattcaaag ccgtacgggt caaaatccag
     7501 ttcatgcatt gctttatggt ggcggtaggt gaggttcaga tcgcgcacca gacgctggac
     7561 accgtggtgc cagttatcgc cgccttccaa cagatgccag tcgaggctgg cgtcatggtt
     7621 ccactcgcgg ccctgggcaa attcgttccc catgaacagc agtttcttgc ccggaaatgc
     7681 ccacatccag ccatagtaag cgcgcaggtt agcgaatttc tgccatgcgt cgcccggcat
     7741 gcggtcgaga atcgattttt taccgtggac cacttcatca tgcgacaacg gcaggacgaa
     7801 gttttcagtg tagttgtaga gcatcccgaa ggtcagttta tcgtgatgat actgacgata
     7861 aatcgggtcg agcttcatgt agtccagggt gtcatgcatc cagccgaggt tccacttgta
     7921 ccagaagccc agaccgccca tatcctgcgg acgagaaacg ccagggaaat cggtagactc
     7981 ctcagccatt gtcaccgcac cggaaacctg ctcaccaaga atacggttgg tattacgcaa
     8041 gaattcaatc gcttcaagat tctcgcgccc gccaaattcg ttcgggatcc actccccctc
     8101 tttacggctg tagtcacgat aaatcatcga cgccaccgca tcgacgcgca gcgcatcaat
     8161 accaaaacgt tcaatccagt aaagcgcgtt accgacgagg aagttactga cttcacggcg
     8221 accatagttg tagatcagcg tgttccagtc ctgatgatag ccttcacgcg gatcgctgtg
     8281 ttcatacaag ttcgtgccat caaattcggc aagcgcaaag tcatcagtcg ggaagtggcc
     8341 tggcacccag tcgagaatca cgttcagacc agctgcgtgt gcggcatcaa tgaaataacg
     8401 gaagtcgtcg cgagtaccaa aacggcgggt tggtgcatac aggccggttg gctgataacc
     8461 ccaactgcca tcgaagggat gctcgttaat gggcagtagt tcgaggtggg taaagcccat
     8521 ccatttagca taaggcacca gttgatcggc cagctcgcgg tagctcaacc agaaattgtt
     8581 gtcggtgtgg cgacgccagg aacccaggtg aacttcataa atagagattg gcgcatcaaa
     8641 ctgattcgct tttttgcgct cttcagtctg tacaaccttt tccggcagcc cgcaaataag
     8701 agacgcggtt tccgggcgca tttgcgcttc gaaggcataa gggtcggact tcagacgcaa
     8761 gttgccattg gcatcaatca tctcgtattt atagagctga ccgttatgcg ccccagggat
     8821 aaacagttcc cagatgccgc tctctttacg caggcgcatc gggtgacggc gaccgtccca
     8881 gtagttgaat tgcccaacca ccgagacccg acgggcgttt ggagcccaga cagagaaacg
     8941 cgtacctgtg acgccatcca tagtatctgc atgcgcgcct aaggtttcat acgggcgcag
     9001 gtgagtacct tcagataata gccaggcatc catttcctgg attagcggac caaaacggta
     9061 aggatcatca atcaggtttt gctgaccatg ccagacaaca gccaactgat agcggaaaaa
     9121 attcttacgt cgcggaatga cgccgctaaa gaatccccgt gagtcgagac actccagttt
     9181 tgcgagtttg cgcccggttt tcggttcaat cacccacaca tcggtagcgt cgggtaaaag
     9241 ggcacggact tccagtcccg cggtggtttt atgcattccc agtacggaaa aaggatccgc
     9301 aaaatggcct gcaattagcg cgttaatcac gtctctatcg atacgatcgg acatgcttgt
     9361 cttcctgttt tattgtgtca ccccatccaa ttggattttt gacttctggt tgtgacattt
     9421 ttttgacctg aacggcgcag cactctgtgc atcctctctg cgtcgtcctc acttcaggta
     9481 aggctgtgaa tactcatgta ttcagccacc cttaaagaat agccaatgct ctatttaact
     9541 cccggtaaat catgaaacat ctgcgcttac tcctgtatta cgcactaaca ggggcggcat
     9601 cgcgccccag atttaatgaa taaagattac gccagttgac gaagcatccg acgcagcggc
     9661 tccgcggccc cccacagcag ctggtcgccc acggtaaagg ctgacaggaa ctctggtccc
     9721 atattcagct tacgcaggcg gcctaccggc gtggtcagcg tgccggtaac ggcagctggg
     9781 gttagctcac gcatagtgat ttcccgatcg ttcggaacga ctttcgccca cggattgtgc
     9841 gcagccagca gttcttcgac ggtcggaata gacacatctt ttttcaattt aatagtgaat
     9901 gcctggctgt ggcagcgcaa tgccccgaca cgcacacata aaccatctac cggaattacg
     9961 gaagatgtgt tgaggatctt gttggtttcc gcctgccctt tccactcttc gcggctctga
    10021 ccgttatcga gctgtttgtc gatccacgga atcaggctac ccgccagcgg cacgccaaag
    10081 ttatccaccg gcagctcacc gctacgggtt aaggttgtga ctttgcgttc gatatcgaga
    10141 atagcagagg acggggtcgc gagttcatct gccacatggc catacagatg gcccatctgg
    10201 gttaataact cacgcatatg tcgcgcacca ccgccggaag cggcttggta ggttgcaacg
    10261 gacacccaat caacaagatc attggcgaat aaaccaccca acgacatcaa catcaggctt
    10321 acggtacagt taccgccaac aaaagtcctg atgccattat ttaatccgtc ggtaatgacg
    10381 tcctgattga cggggtcaag aatgatgatg gcgtcatctt tcatgcgcag agacgatgct
    10441 gcgtcaatcc agtaaccttg ccatccgctt tcacgaagct ttggatagat ttcgttggta
    10501 taatcgccgc cctgacaggt cacaatgata tcgagggcct ttagcgcctc tagatcaaag
    10561 gcatcctgaa gtgtgccagt ggttccgcca aaagacggcg cagcctggcc aagctgagaa
    10621 gtagaaaaga agacagggcg aatggcgtcg aagtcgcgct cttcaaccat gcgttgcatg
    10681 agaacggagc cgaccatacc gcgccagccg ataaaaccaa catttttcat aagcgttttt
    10741 tcctgcaaag atgtgtgctg tataaatgtg ccggtctcct cttggcacat ctttcaccat
    10801 acaaaaagct gccaaagtcg caagtgaaat taatcaatga tagcgaagcc atcagtaatg
    10861 cgacttatcc tgctttgtta gcacgcagaa agtccgcggc aattatcagg gaatttgagt
    10921 tatgaatgaa atcatttctg cagcagtttt attgatcctg attatggatc cgctcggaaa
    10981 cctgcctatt ttcatgtccg tactgaaaca tactgaaccg aaaagacggc gggcaatcat
    11041 ggtgcgagag ttgcttattg ctctcctggt gatgctggtg ttcctgtttg cgggtgagaa
    11101 aattctggca tttctcagcc tgcgagcaga aaccgtctcc atttctggcg gcatcattct
    11161 gtttctaatc gccattaaga tgattttccc cagcgcttca ggaaatagca gcgggcttcc
    11221 ggcaggtgaa gagccattta tcgtgccgtt ggcaattccg ttagtcgccg ggccgactat
    11281 tctcgccacg ctgatgttgt tgtctcatca gtacccgaat cagatgggac atctggtgat
    11341 tgctctgctg ctggcctggg gcggcacctt tgtcatcctg ctacagtctt cgctattttt
    11401 acgtctgctg ggcgagaaag gggtgaacgc acttgaacgc ctgatgggat tgattctggt
    11461 gatgatggca actcagatgt tcctcgacgg cattcgaatg tggatgaagg ggtaacgagg
    11521 agagtttttc gaaaagagcc cggtagcgtc acaactaccg ggcgaacaaa cttaactcaa
    11581 cagctggaac gcaatcatcc caacgatggc accaacagtg ccgaggatgg tttccatcat
    11641 ggtccaggtt ttcagcgttt cggcttcggt cgcgccggta aatttaccga acaaccagaa
    11701 accagcgtcg ttaacgtggc tgacaacaat cgaaccacca gcgatacaaa tcgacagtgc
    11761 cgccatttgc gcaccagagt agttcagttg ttcaatgact ggcatcacca gtcccaccgc
    11821 cgttaaacag gctacggtgg cagccccctg aatgatgcgc actgcagcag ccagcacgaa
    11881 gcaggtgata gcaatcggca ggcccatgcc ggttaacgct tcgcccagtg ccggacctac
    11941 gccagagtca accagcacct gtttgaacac gccgcctgca ccaatcacca gcagaatgat
    12001 ccccgccggt tgcagcgcgt gaccgcaaat ctccatcact ttgtcttttg gcatgccctg
    12061 acgcattgcc aggccataaa tcgctaccag acaagcaacc agaatcgcgg taaacggatg
    12121 accaataaac tcgaaccatt cgtaagcggt agagccttct ggcacaaaac gcgcggcaat
    12181 ggttttcagc cccaccagca ccagcggcaa caggatcagc gacaggctga atccgaaaga
    12241 tggcatttta ccttcgccga gatgcggttc gctgatgtcg tcaggaatat gcagctcaac
    12301 gtaacggctg atgaagttac cccacagcgg cccggcaata atcattcccg gaattgccgc
    12361 acacaggcca atcaggatca tccagccaaa gtcggcattc atctgcgatg ccagcagcat
    12421 tggcgctggc ccaggcacca ggaacgctgc cgcggccgcc acgcctgcga ataatgggat
    12481 taccagcttc accaggttcg taccggtgtg gcgcgccatt gagaaagcaa cgctaatcag
    12541 cagaacaatc gccacttcaa agaacagcgg tagcgcacag accagccccg caaggccgat
    12601 ggcataatgc gcgcggctgt gaccgaagga tttgagcatt ttgacggcaa tctgatcgac
    12661 tgcgccggtt tcatataaga tcttgccaaa catagctccc agggcgacaa ccaccgccag
    12721 gaagccgagg gtgcctccca tccctttttc catcgtcgct gcgattttat cgagcggcat
    12781 accggaaaaa aggccagccc ccatggacac caccatcaaa gccaggaaag cgtgcatacg
    12841 cgccttcatg actaaaaaca gcagcagtaa aacagaccct actgctgtta aaacaagcgt
    12901 taatgtagtc actacttatt tgcctttttt aataacctca atggtgcttg ccacaacacc
    12961 ttccagcggt tgatcgatat ccaccaccag tacatcggtt tcgtccgcac ccggctcctg
    13021 cagcgtttca aactgcgtca ccaacatttg ggttttaaag aaatggcctt tgcgcgcttt
    13081 caggcgactt tcaatcacat caaaatcgcc tttcaggtag atgaaagaga gattcggatt
    13141 accttcacgc agcaagtcgc gatagtgctt tttcagtgca gaacaggcga tcagcgacac
    13201 tttattggtg cgctgcatag caaacgcgga gtcgttcagc gcctgcaacc agggtttgcg
    13261 atcgtcgtca ttcagcggtt cgccagacgc cattttttcg atattgcgcc gtggatggag
    13321 gaaatcgcca tcaagaaacg cggcatgaag ttgatgcgcc acttcactgg cgaccgcaga
    13381 tttgccgctg cccgatacgc ccatcaagac gtaaatgtgg tgatcatggt tagtcgtgct
    13441 caaagtggtg cccccacaat acaagaatta aaaatgttac gggtaactgt tatcggcaac
    13501 attgtccagc cggacaatat cagaagcaat atccatccgt gccttaagtg tataagtgtg
    13561 agctacttca aatttgtggg cttaaataga tccgcccggt gacaaggtga aacctaaatc
    13621 taacattttc ggtgtcacag attcgccacg aatacgcgcc agcaggcgtt cagcgccaat
    13681 actgcccatc cgctcacgcg gcgtcagcac gctcgcaagt cgtggctcca tcacctgacc
    13741 aatgtcatga ccgtggaaac cggcaatcgc catatcgtca ggaactttta accccagacg
    13801 ctgacattca aacgccgcgc cgaccgccag atcatcatta gtacagaaca cgccatccag
    13861 ctgcggatat tcccgccgcg cctggcgaat cagttcaata ccggaagagt aagaagaaga
    13921 ttgctcaacc atcacgctat atggcaccag gcctgcatcc agcatcgcct gttcgtatcc
    13981 cttctgtttg atgatagtac gttcgtcgag acgtgcgccg agataggcaa tgtggcgatg
    14041 cccacgagca ataatggcgg tagtcatctg gcgtgccgct tcaaagttat caaaaccgac
    14101 ggcgatatcg aggcacggcg actggctgtc catcaactcc accaccggga tccccgccac
    14161 ttcaatcatc tttaaggtgc gcggcgtgtg ggtacgttcg gtgaggatca ggccgtcgat
    14221 attccaggag agcatggatt cgagacgttc ttgctccatt tccggtttat aaccgtagtg
    14281 cgccagcatg gtctgataac cgtgcgcgtc ggtgacgctt tcgattccgc gtaatacttc
    14341 cgcgaaaacc tggttggtga gagaaggtaa caggacgcca atcgcccggc tggtagcgtt
    14401 agagaggata tcgggcgcac gattgggaat atagcccagt tcatcaagag cggcggcaat
    14461 cttgccgcgt agagcgacgg aaacctgctc cgggttgcgt aaaaaacggc tgaccgtcat
    14521 tttggtcacg cctacacggt cagccacatc ctgaagtacg ggtcttttct ttttcatcgt
    14581 cctgaaggta caaaagagat agattctctt agtttaacac ggacgcatca gaaccttccc
    14641 ggggaaaaca gtgcttgttt acaattatta gatatacact aaatcaattg catcgcattg
    14701 tgctaacgac gcggcaaaac cccggaagcg tgcatcagca cgcgaccgga gcgagcgagg
    14761 aagcctgcaa aaaggcaggc ttcaaagatg ccgttgagtt ttaaaccggc ggcagatcga
    14821 acagtaacac ttcgctgtcg ctatccgcat ggatggagat tgcctgctca tcccagattg
    14881 ccagaccatc gctggtcgag gctttcacgc cgttaatggt aacattgcct tttaccacct
    14941 gaatccagac gcggcgttca gcggcaatct gatgcaccga ctgctcatct ttcagcaacg
    15001 cccaacgata cagttccata tcctgatgca ctttcagcga accatctcgc gcatccggcg
    15061 agagcaccag ctgtttgccc tgcacggcat cgaagcgacg ctgttcataa cgcggcgtaa
    15121 tgccattttc ttcaggcatg atccagatct gatacagatg cagacgctcg gtgctgcttg
    15181 ggttgtactc tgagtgacga atacccgtac ccgcactcat aatctggaac tcacccgccg
    15241 gaacctgctc tttattgccc atgctgtcct gatgctcaac agtaccttcc agcacgtagg
    15301 tcaaaatttc catatcttta tgcgggtgag tgccgaagcc ctgccctact tcaatcacgt
    15361 cgtcgttaat cacgcgcagc gcggagaagc ccataaagtt cggatcgtaa tagttggcac
    15421 aagagaaagt atgccaggag tccagccagc catgatttgc atgaccacgt tcatttgctt
    15481 tgcgtaagta gatcatttgt attcgccccc tgaatgattt cgatgggctt agtgtggacc
    15541 cgatctgcct gggatgatag agggtgaaaa ttgccccctc tgttcaaaaa aattgaacta
    15601 ttcgaggggc catcaaactt aattcgcgag agtcactgtg gagggagagg cttgctcaaa
    15661 tccgcgttca aggatttcca gattggtaag aacttcagat tccttgacgt aatttggcgc
    15721 accgtgggtg atggtttgat acaacgcatc ataaacacgc ccgtaatcgc ccatctccgg
    15781 cttcatctct tctctgaccg tcacaccctc gtcattgaca tactccagca caccgaccga
    15841 atcatccgct gcgaatcccg gttcgcccgg cataatatta gccttcaggc tggtttcctg
    15901 ctggtcgata ccgtatttaa taaacgaacc tttcttaccg tgaacgataa atttcggata
    15961 atcgattttc accagatggc tggttttgac gatggctttc aggtcgccat aaaacagttg
    16021 cgcttcaaag gtgtcgtcag gattggcttt attacgcagg ctgcggatgt cataagcgac
    16081 gtgatccggg cgaccgaaca gagaaataat ctggtccatc gtatgcacac caaggccata
    16141 gaacgcgcca tcctgcggca gtccaggttt ggtttctgcc accgggcggt aatagtcaaa
    16201 atggctttcc acttcgacaa tctctcccag cttgccgctt tcaatcgctt ttttcgccgt
    16261 caggaagcag gaatcaaagc gacgattctg atatggcgtg acggtcagcc ctttgctttt
    16321 cgccagtgca aacagctctt tcgcctgcgc aagtgtcgga gtgaacgttt tttcgaccag
    16381 cacatttttc ccggcttcca gcgcgcgctt cgcgtactcg aagtggctgt ccgcatgggt
    16441 gcagacaaca accaacttaa catcgggatc gtttagcact tcgtcgagat cgctggtgaa
    16501 atggatatga gagtaaatgg gagcctgttc ttccggcttc gcatggcgac gaaaaatatg
    16561 cgcgacatgc cagctatcct tgcggttaag tacatacggc agatggtaac gggtggtgct
    16621 tttgccgaag ccaataaagg cgcagttgat gaccatgatt tcgtcctttt taaggtggtt
    16681 atttacacct tagcgcaaag cggacgtggt tcctactgga gtgcgcataa cgaacacaag
    16741 cactcccgtg gataaattga gaacgaaaga tcaaaaaaaa agccagcacc cggctggcta
    16801 agtaatactg gaagcaatgt gagcaatgtc gtgctttcag gttctccgcg agggtcttcc
    16861 tgatcgcgat acaataataa tcattctcat tcgcacttgt ccaacacttt ttgcaaaaaa
    16921 atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac cccaaagagg
    16981 acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac gaggccatca
    17041 ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct catccttccg
    17101 atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc gtcgcctgta
    17161 ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcgccca cgccgcagtc
    17221 atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc gtcgccagcg
    17281 ccctgatgcg agagatgatt gaaatgtgcg acaactggtt acgggtagat cgcattgaac
    17341 taaccgtgtt tgtcgataac gcgccagcaa ttaaggtcta taaaaaattc ggctttgaga
    17401 ttgaagggac cggtaagaag tacgcattgc gtaatggtga atatgtcgat gcgtattata
    17461 tggcgcgggt gaaatagaga atttgcaatc taagtttgtc atttaagccc tcacctaccg
    17521 ggagggctta agatattaat accccgccgt taaatcatcc accgagcgcg ggtcggatgc
    17581 gccgtacaac tcaccgtccg gcccaaccat aatgctttgt gtgctgccca tcgcctcttt
    17641 cagcgccact ttctgacctt ttgcttccag cagcttgagc gtatccgggc taaacccttt
    17701 ttcgacacgc agctcgtccg gcaaccactg atggtggaaa cgcggcgcat tggtcgcttc
    17761 ggcgacgttc atgccataat cgatgctatt caccaccatt tgcagcaccg tggtgataat
    17821 ccggctaccg cctgggctac cggtaaccag ccaggtttta ccgtctttca ccacaatggt
    17881 cggcgacatc gacgacagcg ggcgtttgtt cggcccgacg gcgttggcat caccgcccac
    17941 cagcccgtaa acgttcggta cgcccggttt ggcggagaaa tcatccatct ggttattaag
    18001 cagaataccg ctctcacccg cgacaatacc cgtaccgaag gtggtgttca gcgtataggt
    18061 caccgccacc gcgttaccgt ctttatccac tacagagtaa tgggtagttt gattactctc
    18121 ataaggcgca agcttgccag ggcgaatctc actggacggc ttcgcttttt tgatatcaat
    18181 ttgatcggca atggatttgg cataggcttt attggtcagt gcctgccacg ggactttgac
    18241 aaaatccggg tcgccaagat attccgagcg gtcggcgtag gcgtatttct ccgcttctgc
    18301 catgatttgc atcgcatcgg cgctgccaaa gccgtatttc ttcatatcga agttttccag
    18361 aatattgagg atttgtacga tatggatccc gccggaggat ggcggtggca tggagtaaac
    18421 ctgatacccg cgataatcgc cgcttatcgg agtgcgttcg accgctttat aggccgctaa
    18481 atcttcttta gtgatcaagc caccgttttt ctgcatctcc tgggcgatct gttccgcaat
    18541 cgtgccttta tagaattcgt ccgggccgtt ttcagcaatc atctccaggc tctttgccag
    18601 gttcgcctgc accagcgtgt cgcccttttt cagcggctcg ccctctttcc agaagatagc
    18661 tttactgttt tcgtgattcg gcaacacttc gctaccgtag gttttgagat cgtcagccag
    18721 cgcgtcgtta acgataaaac catcgcgtgc cagtttaaac gcgggctgca cgactttgtt
    18781 cagcggcatg gtgccgtatt tatccagcgc cagcgagaaa cctgctaccg tacccggtgt
    18841 gccggaagcc agatgcgaag tgagtgattt tttgctgtcc gggttgccct gatcatcgag
    18901 gaacatatcg cgggtcgctt tggcgggtgc catttcgcgg aaatcgatag ccgtggtatt
    18961 gccatttttc gagcggatta acataaaacc accaccgccc agattccctg cctgcggatg
    19021 cgttaccgcc agcgcgtagc ccaccgccac ggcggcatca acggcattcc cgccctcctt
    19081 gaggatatcc acccccacct gagtggcagt ggcgtccaca gacgctacca ttccctgttt
    19141 cgcgcgtacc ggatggaaga catcttcctc cacaccatac gagacgggcg gcgcaggagg
    19201 cgcggcggcg gcgctaaaac aacttcctga gagcagagca gcaatggcca cccggcgtaa
    19261 aaacgtcggt tttatcatcg ttattctcca gagattaagg ggcaacccca gctaagcctg
    19321 gtatataact ctgaattaat catcgttttg tcgggaagcg agtaaactta aaggatatct
    19381 tcagaggagg atacgcgatg aaacgacttc tgattcttac ggcactcctg ccgtttgtcg
    19441 gctttgcaca gcccattaat actctgaaca accctaacca gccggggtat cagataccca
    19501 gccagcagcg gatgcaaacc cagatgcaga ctcagcaaat ccagcaaaaa gggatgctga
    19561 atcagcaact gaaaacgcaa actcagttgc aacagcagca tttagaaaac cagataaaca
    19621 ataattctca gcgggtgttg cagtcacagc cgggggagcg aaatcccgcc cggcagcaaa
    19681 tgctgcccaa caccaacggc gggatgttaa acagcaaccg taatccggat agttcgttga
    19741 atcagcagca tatgctgccg gagaggagaa acagcgacat gctgaatcag cccagcacgc
    19801 cgcagcctga cattccgttg aaaactattg ggccgtaaag ttcggaccaa tcacgtcaat
    19861 cgcatcggta cagatgcaat ccacgcccca gcgcagcaac tctgccgcgt gctggggttt
    19921 attgacggta taaaccagaa tccgtaatcc ggcgtctttc aactgcatca ctcgcgcttt
    19981 atcgagtaac ttatgattga gatgaataga gacgcagccc agccgcgcgg tcagttcgcg
    20041 ccagtcgtcg cgccactcat caagcaataa accacgcggc agttccggtg ccgcctgttg
    20101 tgcagcttct aaagcatcaa tctcaaacga tgacagcagc ggcggcgtca taccggccca
    20161 cagttggcgt gccgccagcg ccaccatttt gcccgttaat ggtccagtgc cagtggtggg
    20221 tttgatttcg atattcgcca tcatcccgtg ttcgcgacag cgttccgcca cctgcgaaag
    20281 caacggcagc ggctcacctt taaacgcttt gctgtaccaa ctgcccgcat ccacgcgcag
    20341 taaatcctgc cagttcagtt cacccgcgac gccccagccg ttgctggtgc gttcgagatt
    20401 gtcgtcatgg agcaggaaga tctcgccatc tttcgataac ttcgcgtcaa attcgatcat
    20461 cttatgaccg tattttgccc cgacgtcgat tgccgccagg gtgttttccg gggccagctt
    20521 accgccgcca cgatgagcga cgatgcgggg ataaggccag ttactcatac tcgttgtcct
    20581 gtttcaccat caaaaagatg cagctgattt tccgccagat gcagccacag cgtgctgcct
    20641 gccgtcgggc gctcctgatg cgccagtcgc accaccagct tctgcttgcg agcttagcgc
    20701 aatatgttcc gggcgaatgc cgagggtcat tttacgcccg gcatactgac ggtagccacc
    20761 gtttagcggt agcacgatac cgccgtccag ttcgaaatgc gtgccttcat tattcatgcg
    20821 gcctgtcagc aggttcatcg ccgggctgcc gataaaactc gccacaaaca ggctggcggg
    20881 cttttcgtag acttccaccg gcgtgccaat ctgttcggca acgccgccgt tcatcaccat
    20941 tactcgctgg gcgagcgtca tcgcctcaac ctgatcgtga gtaacgtaga gtgaagtcgt
    21001 tttcaggcga cggtgcagct gttgcagttc aagacgcatc tgtacgcgca gcttggcatc
    21061 gaggttagag agtggctcat caaacaagaa caccgccgga tcgcgcacaa tcgcgcgtcc
    21121 catcgccaca cgctggcgct gaccgccgga aagctcgcgc gggcgacgtt tgagcagacc
    21181 gtccagctcc agaatgcgcg ccgcttcttt aacgcgctcg gctatttgct gcttgcccat
    21241 gccgcgaatt ttcagccccc acgccatgtt ttcttcgacg ctcatatgcg gataaagcgc
    21301 gtaattctgg aacaccatcg caatcccgcg atctttcggc tccatttcgg tcacgcgctg
    21361 gtcgttgatc cagatatcgc cttcggtcac cctctccagc ccggcaacca tacgcagcag
    21421 cgtcgatttc ccgcagccag acggcccgac catcacgata aattcgccat ccgccacatc
    21481 aagggtcagc ggtttaatga cctaggtttt gccatcccag cttttggtta ctgcctgtaa
    21541 tttcagtcct gccatcttat ttctcactat cgaccaggcc gcgcacgaag gcacgctgca
    21601 tcactaaaac aatcactact ggtgggatca gggttaacaa catcgccgcc atcactgagt
    21661 tccattccgt ggtgccttcg ccagtggcga tcatcccttt gatccctgcc acggtggtgc
    21721 cgagatccac gtcggtaata atcaacaacg gccacaaata ctggttccag ccgtaaataa
    21781 aggtgatcac aaacagcgcc gccagattag ttttggagag cggaaaaacg atgtcgcaaa
    21841 agaagcgcat tggtgacgca ccgtcgatcc gcgccgcttc caccagttca tccggcagcg
    21901 tcataaagaa ctggcgaaac agaaacgtgg cggtcgccga ggccatcagc ggcagcgtta
    21961 aaccggcgta gctgtcgagc atcttcaggt tggcgatgac ttccaccgtc gggaagatac
    22021 gtacttcaac cggcagcatc agggtgataa aaatcatcca gaagaagagg ttacgtagcg
    22081 gaaaacgaaa ccagacaatg gcaaatgccg agagcatcga gacagtaatt ttgccgagcg
    22141 taatgctgaa cgccatcaca aagctgttaa gcaacatccg ccagaacggc gcgctatttg
    22201 tgcctacccc gttcacccag atgttgtgga tgttttccag cagatgcgtg ccggggatga
    22261 gcgtcatcgg cgcggcgtag acggcctgtt tatccagcgt cgccgcgaca aacgccacgt
    22321 acagcgggaa gaggatcacc gcgatcccga ggatcagcat ggtatggctg aatatcgtca
    22381 gccacggacg gttctcaatc attggtaacg caccttgctt tcaacatagc ggaactgcac
    22441 caccgtcagc acgatgacga ggaacatcag caccatcgac tgtgcggcag acgaagccag
    22501 atccagtccg gtaaaacctt cgcggtagat cttataaatc agcgtcgtgg tggcctgaac
    22561 cggcccgccg gacgtggcgg cgtcgatcac cgggaaggtg tcgaagaagg catacaccag
    22621 gttcactacc agcaggaaga aactcaccgg ggcgataagc ggcagcgcaa tcttaaagaa
    22681 gcggcgaatc ggccctgcac cgtcgatggc tgcggcttcg atcaacgaac ggggaatgga
    22741 ttgcagcgcg gcatagaaga acagaaagtt gtagctgatt tgcttccaca ccgaggcaaa
    22801 caccaccaga aacatcgcct gaccgctatt ttgcgcgtgg ttccagtcgt agccgaactc
    22861 agcgagaaaa tgggtgatca gcccgcgacc ggggttaaac aggaagatcc acaatacggc
    22921 ggcaacggcg ggagccacgg catacggcag caacattaag gtttggtaga aacgactgcc
    22981 gcgcacgatg tactccacca gcgccgcaaa gaacagcgat accagcaaac cgctgacggt
    23041 gacaaaggtg ctgaatttta tcgtcgtcca gaaggagtcg agataatagc tgtcatgaaa
    23101 cagcgtgacg aagttatcca gaccgacaaa ctggctggag aaaccaaacg gatcgacgct
    23161 ttgtagcgag taccacaacg cttcgcccgc aggccagata aaaaagataa cggtgatgat
    23221 gagctgcggc gcgaccagca gataaggcag ccagcgcgag cggaacaccg gacgggatga
    23281 tgacattacg gttaattcct gaactgtgcc ggatgcgctt cgcttatccg gcctacacgc
    23341 gatgccaggt cagataagcg aaacgcatcc ggcattacac tgattaagac ttcgtcgatt
    23401 tctcaaagcg gcgcagtaac tggttcccac gctcaactgc ggtatccagc gcctgctgtg
    23461 gcgtcttctt accggtccac acgctctcaa gctcttcatc cacaatcacg cggatctgcg
    23521 gcatgttgcc cagacgaagc cctttggtga acggcaacgg cggcttgttc agcatctgac
    23581 gcgtcgcggt atccgcacct gggtttttct cgtaaaagcc ctgctcacgg gtcaggtcat
    23641 acgccgcttt ggtaattggc agataaccgg ttttctgatg ccactcagca gcgttttctg
    23701 gcttcgcgag gaagtcgaga aacttcgcca cgccggtata cgtttcttta tctttgccct
    23761 gcatcaccca caggctggct ccgccgataa tggcgttttg tggcgcatct ttcgcatcgg
    23821 cgtcgtaagg catcatacct acgccgtagt taaatttggc gtactcgcga atgttggcaa
    23881 gagaaccgga agaggcggtg gtcatcgcgc aatcaccgtt atagaacttc tcggtggatt
    23941 catccttacg accgacgtag ctgaagtcgc ccttcttgtt catctcctcg agcatggcga
    24001 tgtgtttcac ctgctccggc ttgttgaact ccagcaccgc gtccgtgccg tcaaagccgt
    24061 tgtttttgct ggcaaacggc agaccgttcc aggcgctaaa gttttccagt tggatccagc
    24121 cctgccagcc gctggcgtag ccgcacttaa taccggaggc tttcagtttt gcggaatagt
    24181 ccgccagatc ctgccaggtt ttcggcggct gttccgggtc taaccccgct ttcttgaagg
    24241 cgtctttgtt gtaatagaga acggaagtcg agctgttgaa cggctgggag agtaagtggc
    24301 cggttttgct gtcggagtag taaccggaaa ccgtcggcac aaactgcgac tcatcgaact
    24361 gaatccctgc ctctttaaac acgtcataca ccggtttaat ggctttcgac gccatcatgg
    24421 tggcggtgcc aacttcataa acctgcaaaa tagccggagc gttgccggta cgaaatacgg
    24481 caatccccgc gcttaaattc tgttcgtagt tgcctttata ggtcggtaca attttgtaat
    24541 ccggattttc ggcgttaaaa cgttgggcca gagaatccac ctctttaccc agttcccctt
    24601 ccatagaatg ccagaacgga atggtcgtca ctgcctgtgc attccccatt aacgccagtc
    24661 cgagcgccag tgctgaagct gtataatgta acggtttcat cgtttatctc tcttgttgta
    24721 ccgaatgcgc gaattcacgc gttttatgct cgcggggtaa catgacatgc tcgaattaca
    24781 gaaaaataac ttttttgtta catttgtaag atagtaaggt gtcagaaaga tgacaaggcg
    24841 gtgacggcgt gggtgaggga aaatgggaga tggggcacgg ataagcggaa aaatagaagg
    24901 tctgaatcaa actctacaga ttactcatcg tttcatgccg gatgcggcgt aaacgcctta
    24961 tccggcctac aagatcgtgc aaattcaata tattgcaact cacccagtag gcctgataag
    25021 cgcagcgcat caggcaattt tgcatttgtc acctgtctca aaggagtctt ttgactcctt
    25081 atcaatcaac gtgttattac ccgcctaaat acgcacttct cactgcttcg ttcgccagca
    25141 gcgcgtcgcc ggtatcggag agcaccacat ggccgttttc cagcacgtag ccgcgatccg
    25201 ccagctttag cgcctggttg gcgttctgct cgacgaggaa gatagtcatc ccctgctcgc
    25261 gcagctgctc gatggtgtcg aaaatttgct ggatgataat cggcgcaaga ccgagcgatg
    25321 gctcatcaag taacaacaaa cgcggattgc tcatcagcgc gcgaccaatc gccagcatct
    25381 gctgttcacc gccggacatg gtgcccgccc gctgaacacg gcgctcatgc agacgtggaa
    25441 acagttcata cacccacttt atgcgctcct gaaactggtc acgttcggca aagaagccgc
    25501 ccatcgccag gttctcttcc accgtcatcc gcgagaagac gcgacgccct tccgggacaa
    25561 tcgctaccgc ttcgcgcatg attttcgccg tctgccagtc ggtaatgtct ttatcatcaa
    25621 acacaattcg cccgctggtg gcgcgcggat cgccgcataa cgtgccgagc aaggtggttt
    25681 tccccgcccc gttcgcgcca atgagagtga caatctcgcc ctgattgata tgcaggctca
    25741 cctcatgcag cgcctggatt ttgccgtagt gggcgctgac tttgtcaaag gacaacatga
    25801 ctttttccat cttatgcctc acctaaatag gcacggatca cgtccgggtt attacggatt
    25861 tgctccggcg taccgtttgc cagtggcgtc ccctgattga ccacataaat tcggtctgaa
    25921 atccccatca ccagcttcat atcgtgttca atcaacaaaa tagtggtgtt gtgatgattg
    25981 cgcagttcgg caatcagctc atccagctct ttggtttctt ttgggttaag acctgccgcc
    26041 ggttcgtcga gcattaaaat ctccggctgc gtcaccatgc agcgggcaat ctccagacgg
    26101 cgttggtcac cataggccag gttactcgcc tgacggttgg cgtgttccag caaaccaatg
    26161 cgctcaagcc aggtggcggc gcggtcgagc gcttcgctct gggcgcgacg gaaagaaggc
    26221 gttttcaaca ggccagagaa cagcccggtt ttcagttgct gatgctgcgc caccagcagg
    26281 ttttcaatta ccgtcatttc acggaacaga cgcacatgct ggaaggtgcg caccacgccc
    26341 atgcgagcaa tttgctgccc cggtaaacct tcaagatgct gatcgcgcag taaaatggtg
    26401 ccgccggtgg gtttgtagaa accggtcagg cagttaaaaa ccgtggtttt tccggcaccg
    26461 ttagggccga ttaacgagac gatttcctgc gggtacagtt caagattgac gttgttcacc
    26521 gccagcaggc cgccgaagcg catcatcaga ccgttaacag ataataatgg ctgactcatg
    26581 cctgctctcc tttcgctgcg ccgtttttca gcttcagttg cgggcgcgtc atgggcagca
    26641 aaccctgcgg acgccagatc atcatcagca ccatcaaacc accgagcatt aacatgctgt
    26701 attcgttgaa atcacgcatc aattcgcgcg acaccaccag cagaacagcc gccagaatca
    26761 ctgcaaattg cgagcccata ccgccgagca ccactatcgc cagcacaaag gccgattcag
    26821 caaaggtgaa ggattccggg ctgacaaagc cctgacgcgc cgcaaacagc gttccggcaa
    26881 aaccggcaaa tgcggcgctg atggtaaaag cagtcagctt gatacggcgc gggcttaagc
    26941 ccagcgaacg acaggcgatc tcgtcttcac gcaacgcttc ccacgcgcgg cccagcggca
    27001 tccgcagcag gcggttaatg acaaacaggc ttaacaccac cagcagcaac gccaccaggt
    27061 agaggaagat aacgcgatcg gacggatcgt atttcaggcc aaagaaatta ctgaacgtgt
    27121 cccagccgcc ttcacgagcg gtacggctga actcgagtcc gaagagtgtc ggtttcggga
    27181 tctgactgat tccgttcggg ccgccggtga tttcagtgtt attgagcagc aatatgcgca
    27241 caatttcgcc gaaaccaagg gtaacgatcg ccagatagtc accgcgcaga cgcagcaccg
    27301 gaaaaccgag caggaaaccc gccgccgctg ccatcagtcc ggcaatcggc aaacaggtcc
    27361 agaaccccag accgtaatag tgattgagca gcgcaaaagt gtaagcgccg atggcgtaaa
    27421 aaccgccgta ccccagcacc agcagaccag aaagaccaac aaccacattc agcccgagac
    27481 cgaggataat gtagatcatg gtcagagtgg cgatatccac cgtcccacgt gacaccataa
    27541 acggccacgc caccacaagc accagcagcg ccacgaggaa cagcttttgc ttcaccgtgg
    27601 acccatcaat cgcgggcaga ataaacttcg gcccggaaac gcttttcaac cctttctgga
    27661 aagccggtcg caaaagctgg aagaaaaaga ccaccgccgt gccgataaac atccactgcc
    27721 aacggatgtc tgaagccgtg tcgaccacca gtttggtgcc atccagctcc agttgtacgc
    27781 ccataaagac gcccgccagg acaaagaaca tcgcggcaga gagcagtgcc attgcaatat
    27841 gcatcggttt catactttct ctacctccgg gcgacccaga ataccggtcg gcatcaccag
    27901 cagcaccaga atcagcaggg cgaatgagac cacatcttta tattccgtac tcagataggc
    27961 agaagagagc gcctccgcaa tccccaggat cagcccgcca atcatcgccc ctggaatgct
    28021 gccaatccca ccgagcaccg cagcggtaaa ggctttcatc ccggccataa agccgatata
    28081 ggggttaatg acgcagtaga actgaccgag cagtacgcca gccactgccg ccatcgccgc
    28141 gccaataaca aaggtcaaag caatcacccg gtcggtgttg atgccaagca ggctcgccat
    28201 tttcagatct tccgcgcagg cacgacaggc gcgccccata cgggaatagc gaatgaaaat
    28261 cgtcagcgcc agcatggcga ggaaggtgac aatccagatc accgcctgca tggtggtaat
    28321 agaggcagag aagttttcgc tatggcccac cacccactga ccgttaaaca ggctcggcag
    28381 cgccacgtcg cgcgaacctt cggtcaggct gacgtagttt tgcaggaaga tggacatacc
    28441 gattgcggaa ataagcgcaa tcaggcgctt agagttacgc accgggcggt aagctacccg
    28501 ttcgatactc cagccgtagg cgctggcgat gacgattgcg ccgacgaatc ccgccgccac
    28561 cagcagccag ccggtatcaa tgcccatcat catcagcgcg gcgatgatca taaatgagac
    28621 gtagctgcca atcatataaa cctcgccgtg ggcgaagttg atcatgccga taatgccgta
    28681 aaccatggtg tagccgatgg ctatcagcgc gtaggtactg cccagcgtga cgccgttaaa
    28741 catctgctgc aagaaataca aaaactgctc agacataagg taacctttct aaacccgccc
    28801 gcattttacg ggcggtggga tgatcacttg gctgccgtgg atgaaccgtc ggcgtgccac
    28861 tggaagacac caaaatcaaa tcccttaaga tcgccttttt catcccagtt cagcggccca
    28921 atcacggtgt ttgcaccgtt agcttttaaa tctttcacca gcgccagcgg ctcatcgctg
    28981 ccggtacgct caagggcagt tgccagagat tgcactgccg cgtaagtgat ccagacataa
    29041 ggcccggacg gatctttctt gtctgctttc agcgcatcaa cgatgccctg gtttgccgga
    29101 tcctggtcat agcgttttgg catagtgacc aacatgcctt cggcggcatc accggcaatg
    29161 ttcgacagcg atgcgttacc gacaccttcc ggccccataa actgggtttt caggccaacg
    29221 gaacgggctt ggcgcagcat ctgccccatt tccgggtagt aaccgccgta gtaaacgaag
    29281 tcgatatttt cttttttcag gcgggcgatc agcgcggaga aatctttctc cccggcggta
    29341 ataccgtcga agaagacgac gttggcgtta gccgctttca gcccgtcctg caccgaacgc
    29401 gccagccctt cgccatactg ttgtttgtcg tgaatgatgg cgatgcgctg gggcttcacc
    29461 gtctcaagaa tgtattttgc cgccgtaggc ccctgggaag agtccagccc ggcagtacgc
    29521 ataatgtgtt gataaccgcg ttgggtcagc tccgggttgg tcgctcccgg cgagatcatc
    29581 agaatacctt cgtcttcgta gatatccgac gctggctggg tagaggaaga acacagatga
    29641 ccaataacgt atttaatgcc gtcgttaacg attttgttgg cgaccgcaac ggcttgtttc
    29701 gggtcgcagg cgtcgtcata ttccacgcca accagcttgt cgcccttaat tccccctttg
    29761 acattaatgt ctttaatcgc ctgacgcgcg ccgttaaatt ccatatcgcc ccactgggca
    29821 atcgggccgg acatcgcgcc gacaacggcg actttaatat cgtcagccat agcggtgtgt
    29881 gaaattgcca gtgcaatcat ccctgcgatg atagttttcg cattccgttt catagtcaaa
    29941 aatccccatt cgtgatgttg tgttgctttg tttttatgtg ttaacaaatc agactgttct
    30001 ttttttatac tgcactgttt ttgcctgtct gattaagggg ttagcgcagt attttgtgat
    30061 gatagcgatt aaaatcccta tttttcagtc gattaagaac agataatatt ctgaatttat
    30121 tgatagataa acagaaaaaa gtgcctttgt cagcataaaa taacggcaca aagggcggaa
    30181 taattcacta tcattcaggg gattatgctg gacatttttc attctctaat gttttaactt
    30241 tgtaattatt gctgttaaaa aattaatcac ctgccaaaag aaataaaaaa gagaaagcct
    30301 ccgattaaat tatttcgcta cactggttcc acttttgtga tttacacggg ttacccatga
    30361 agctgaccat cattcgatta gaaaacttta gcgaccagga ccggattgac ctgcaaaaga
    30421 tctggccgga gtattcccct tcctctttac aggttgacga taaccaccgt atctacgccg
    30481 cgcgttttaa cgagcgtctg cttgcggccg tgcgggtaac cttaagcggc accgagggag
    30541 cactggattc tctacgcgtg cgggaagtca cccgccgtcg cggtgtgggg caatatctgc
    30601 tggaagaggt tttgcgtaac aatcctggcg tttcatgctg gtggatggcg gatgcaggcg
    30661 tggaagatcg cggtgtgatg acggcgttta tgcaggcact gggatttacg gcacagcagg
    30721 gcggctggga gaagcgttaa tcgtcaagtt gatttcaaaa gtgatgacca acgagaaatt
    30781 gcctgatgcg ctacgcttat caggcctata atgtgtgctg caatttactg atttctttgg
    30841 ttcttgtagg ccggataagg cgtttacgcc gcatccggca tgaagcaacg tattcgatat
    30901 tagcaatttg gcggcaaccc aaagttgccg attaacaatt acttcgcatc ggtcgccgtg
    30961 ccgttggcgt gccagtcaaa tacgccgaac tcaaagccct ttagatcgcc tttctcatcc
    31021 caggtcagtg gccccatcac ggtatccacg gagttcgctt ttaggtattt ggcgatttca
    31081 gccggatcgt cagactgatt caggcccgcc tgcaaagatt gcagcgcggc gtaggtggtc
    31141 cagacgaatg cgccgcttgg gtcctgtttt ttcgctttga tcgcatcaac aatgggtttg
    31201 ttcgccggaa cctgatcgta gttcttcggc ttggtcacca gcagcccttc cgctgattcg
    31261 cccgcaatgt tagacagcga aacgttagcc acaccttccg gccccataaa ctgagttttc
    31321 agccctgccg cgcgtgcctg acgcaggatt tgccccattt ccgggtgata accgccgtag
    31381 taaacgaagt cgatattctc ttttttcaga cgcgccacca gcgttgagaa atctttttcc
    31441 ccggcggtga taccatcaaa gaacaccacg ttagcattgc ctttcttcag gccgtcctgc
    31501 accgctcgtg ccagaccttc gccgtattgc tgtttgtcgt gaacgatagc aatacgctgc
    31561 ggtttcactt tctcaagaat atatttcgcc gccgtcggcc cctggtcgga gtccaggccg
    31621 gtggtgcgca ggatcagctg atagccacgg gcggtcagct ccggcgcggt tgccgctggg
    31681 gtgatcatta aaatgccttc gtcttcgtag atgtcagacg caggctgcgt tgatgaagaa
    31741 cagaggtgac caatcacata tttaatgccg tcgttaacga ctttgttcgc caccgcaacc
    31801 gcctgtttcg ggtcacaggc atcgtcatat tttacgattt gcagtttgtt gcctttaatg
    31861 ccgcctttag cgttgatatc cgcaaccgcc tgctctgcgc cggtaaactc ctggtcaccg
    31921 tactgcgcaa ccggaccgga cattgcaccc acgaccgcga ctttaatatc ttctgccaga
    31981 gccatattgc tgaatgccag cgcgatacat cctgccagta acgctttacc ctttatgttc
    32041 atcctgagaa tccccattct tctggttatt acgtgtgttg tgatgttgtt gttcagcact
    32101 ttatttcgtt ttatgcatga ctacccgtgc tttagcagca tactctgcta aaacataccc
    32161 gatttttatg atattggaat agctattttg acagtttatt aacaatctgc gtggggattg
    32221 gcgttttgcc ggagggggaa ttgattattg tagaggccca aaaaacaaaa accccggact
    32281 ctcatccagg gttctctgct taatagcgga aattacgctt caatagcggc gcgaagtttt
    32341 ttcatcgcgt tcttttccag ttggcgcaca cgttcagcgg aaacaccgta acggtcagcc
    32401 agttcctgca acgtggactt gttgtcttcg tccagccagc gcgcacggat gatgtcctgg
    32461 ctgcgttcgt ccagaccatg catcgcgtcg gtcagacggt ttgccgcctg ctcttcccag
    32521 ttatcatctt cgatgccgtc ggcaaagtta gatgatttat cctgcagata gagcaccggt
    32581 gccatcggct ggctgtcgga atcgtcgtcg gaagacaggt caaaggtcat gtcctgtgcc
    32641 gccatacgtg attccatctc acgtacgtct ttgctggtta cgcccagttc acgggccacc
    32701 atttcgactt catcctggtt aaaccagccc agacgctgct tggttttacg caggttgaag
    32761 aacagtttgc gctgcgcttt ggtggtcgca actttgacga tacgccagtt acgcagaacg
    32821 tattcgtgga tctctgcttt gatccagtgg acggcgaagg agaccaggcg cacacccact
    32881 tccgggttaa aacggcgcac tgctttcatc aggccgatgt taccttcctg aatcaaatcc
    32941 gcctgtggca ggccatagcc cgcataatta cgagcaatat gaacaacaaa ccgcaggtga
    33001 gacaggatca gcgttttagc tgcttccaga tcgccatggt aatgcagctt ttcagccagc
    33061 gcccgctcct cgtcagccga caacatcggc cacgcgttag ctgcccggat gtaggaatcc
    33121 aggttgccaa ctggggctaa agctaaactt tgcattttgt cagtcattca aatcctctca
    33181 atcgatatct tctggcgctt cagtggtagc aacaactgtg ccaaagctta agagcaacga
    33241 ggttatcatt cactgtttta tcagaccgtg attttatcca caagttcaat gcaagcttgt
    33301 gaataaatta cgcacaaaat gtgacataga gatgaaatac cgggaagaga caacggggtc
    33361 tctttccctg ctacggaacc cattgcaggg aaagagtata acacgctttt attattcagg
    33421 cgtaaagtgg cgtaaatgtt gtaccgtggc aagccacgct gccacccagc caatcatcga
    33481 gcataccagc agcaatagca ggcattcatc gaacgataaa ccattgatat caaacttcgt
    33541 tccgaaaacc tgtgccactt ccgccaccgc tgacgacaaa cgcagtacca gaatttctga
    33601 caaaattaat gacaacaatg cgccagaaaa tcccagcagc gcaccgccgt acaggaacgg
    33661 gcgaaggatg aaaccatccg tcgcaccaat cagtttctgc acgttgatgg agtcacggcg
    33721 agcaaagatg ctcagacgta cgctgttgcc gatgacgagg aacactgccg ccaccatcaa
    33781 cacgccgatc atcgccgaaa cgcgcccaac cagcccggtc aacgccgcca gacgggcaaa
    33841 ccagctgtca tccatccgca cttcgtcaat accgttaatc tgcgtgatac gatcacgcag
    33901 cgtattgagt gattccgtcc cctgaaaatc gagtttcggg atcaccaccg ccactgccgg
    33961 aagcgggttt tcttccagca tatccagcgc accaccaaaa ccagaccagt tacggaactc
    34021 gcccagtgcg tcttcacgag aaagatagtt cactttctcc acgccttgct cggcctgcaa
    34081 ctgtgccacc acgcccgcag cggcgtcatc gtccagcgtt ttttgcaagt aaacagtgat
    34141 ttgcggtgac ggataatact gcgtcgccgc ctgattaacg tttttgtaca ccatataaca
    34201 gacgctgggc agcgtcaggg agatggcgat aaccatcacc gttaaaaacg tggcgaacgg
    34261 tttgcttttc agatcctgca atgcgccgtg gaaggcatag cgcacctgtt cgttgaaaac
    34321 gttggttttg cgatttaccg gttttggcga ggatttcgcg cgtttcggtg cgttacgacc
    34381 gccgtcgcct gagccgccga ccgatttacg gaagcgatcc agacgcccac caaactgccg
    34441 aatatgattg attgcatcgc gcttattcat ggcccacgcc tccatgcaag tgaccatcgc
    34501 tcagggtgag catgcgatag gaacgccgtg aaatcagatt aatgtcgtgc gttgccatca
    34561 atacggttac cccaacgcgg ttaaactctt caaacagacg tagaatccct tccgacaacg
    34621 cgtcgtccag gttaccggtc ggttcgtccg ccagcagtac cgcgggctta ttcaccaccg
    34681 cgcgggcaat gccaacacgc tgttgttcac cgccggaaag ctgaataggg aagttcttcg
    34741 ctttgtccag tagcccgact ttatccagtg ccgccgacac ccggcgacga atatcgtcac
    34801 cgctggcacc agcgataatc agcgggatcg ccacgttatc gtagacagta cggtccatca
    34861 gtagatggtg atcctggaaa atcatgccaa tctggcggcg cagaaacgga acttcacggt
    34921 ttttcagacg cgtgatgtca tggccgctaa accagatttt cccggcgctg ggccgctcaa
    34981 tcccacagat cagcttcagg agggtacttt tccctgcgcc ggaatgaccg gtcagaaacg
    35041 ccatctcacc cggctgcata tggaacgtaa cgccctgcag cgcctgtctc ccaccgagat
    35101 aagccttgct gacatgttca aagcgaatca ttgttaatcc tctcgggcaa aaagtgcctc
    35161 tataaagtcg tccgccttaa acggacgcaa atcctcaata cgttcgccga caccaatgta
    35221 gcggataggg ataccaaact ggtcagccac cgagaaaatt accccgcctt tcgccgtgcc
    35281 gtccagtttc gttagcgtga tgccggttaa gccaacagct tcgtggaaca gtttggcctg
    35341 gcttaccgcg ttctgcccgg tgctggcatc aatagtcagc ataacttcat gcggcgcttc
    35401 aacgtcgagt ttcttcatca cgcggacgat tttcttcaac tcttccatca gatgcgattt
    35461 gttctgcaga cgtccggcgg tatcggcaat caggacgtcg atattacgcg ctttagctgc
    35521 ctgaatggcg tcgaagataa cagaggcgga atccgcaccg gtatgctggg caatcaccgg
    35581 aatattgttg cgctgacccc agacctgaag ctgttcaacc gcagctgcac ggaaagtatc
    35641 gcccgccgcc agcatcaccg atttaccctg ctgctcaaac tgacgcgcca gcttaccaat
    35701 cgtcgtggtt ttacccacac cgttgacgcc caccatcagg atcacgaacg gcgttttatc
    35761 ttcaacattc agcggctcat cgactttcgc cagaatctcg cccatctctt ctttcagcag
    35821 gccatagagc gcctcggcat cacgaagctg cttgcgggat gcgccttccg tcagattggt
    35881 gataatttta cgtgtggttt ccacgcccac atcggcgatc aacagctgtt cttccagctc
    35941 ttcaaacaga tcatcgtcga tttttttacc gcggaacagg ctgataaatc cggaaccgag
    36001 attttctttg gtttttaaca ggctgcgttt caggcgcgcg aagaagcctt ctttggtcgg
    36061 tttttcttgc tcctgggcaa tttctgcaac cggctgctct tcttctgccg gaggaactac
    36121 catcaccgcc tcttctgccg cttcggcagc cagcgcctgt gcttccagct cttcgtcggt
    36181 gatttcttct ttagccgctt cttcttcttc tgccgcttcg acaatctcta cggtttccgc
    36241 ttcagcctgc cactcttctg acgaaacctc ttcggcgttg acgtcttccg gcaacggcag
    36301 ctcttcacgt tcgatagcca ctggctccgg cgtttcttct acgaccggtt ccggctgtgc
    36361 aacaacttcc gcttcaggct gcgctttttc actttcaaca acctgttcag tgacttccac
    36421 aacgtcggca gcaaaagttt ccgcctcggc ttcagtatgc gcctgcggct gctcttcaac
    36481 ggcttgttca gaagccttcg caggctcttg cgcctgaacg atttcttcta caaccggttg
    36541 ttcattctga acttctgtct ctttttccgg ggtctgctct ttttgaccaa agcccagcca
    36601 ggaaaaaaag ccacgtcttt tttctttcgc catttgcgac tacactcctc gctgttgctt
    36661 catggcacag cgttaacgct atgtacatag cagctaaaaa aatgatgaaa tagtctatca
    36721 cttaacttaa ttcacatcac cgcctgcaag tatgtgttat ctggcggatt gagcaattta
    36781 tcatgaaaaa accgaatcat tccggcagcg gccaaatccg cattattggc gggcagtggc
    36841 gaggccgtaa actcccggtt cctgatagcc caggtctgcg ccccaccacc gaccgcgtac
    36901 gcgaaacgtt gtttaactgg ctggctccgg tcattgttga cgcccaatgt ctggattgct
    36961 tcgccgggag cggcgcgctg gggctggaag cgttatcgcg ctacgctgcg ggggcaacgt
    37021 tgattgagat ggatcgcgtg gtttctcagc agttaattaa gaatctggcg acactaaaag
    37081 caggcaatgc acgcgtggtg aacagcaacg caatgtcatt cctggcgcaa aaaggcacac
    37141 cgcataatat cgtgtttgtc gatccaccgt tccgccgtgg cttgttagaa gagacgataa
    37201 atttactgga agataacggc tggctggctg acgaagccct gatttatgtc gaaagcgaag
    37261 tcgaaaacgg tctgcccact gttccggcaa actggtcatt acatcgggaa aaagtggcgg
    37321 gtcaggtggc ttatcggctg tatcaacacg aagcacaagg agaaagtgat gctgattaat
    37381 attggtcgtt tgttaatgct ctgcgtttgg ggatttttaa tcctcaatct ggtgcatccc
    37441 ttcccgcgcc cgctaaatat tttcgttaac gtggcgctga tttttaccgt gctgatgcat
    37501 ggtatgcagc tggcgctatt gaaatccact ttaccgaaag atggcccgca gatgaccacc
    37561 gccgaaaagg tgcggatttt ccttttcggc gtgtttgaac tgctggtctg gcagaagaaa
    37621 tttaaagtta aaaaataacc ttattgttcg ccaacaaagc taacaaagcg cgttccttta
    37681 tagctcagcg tacctttatc gcccactgtc agggcgtggt actgctgggc gtcgaggcga
    37741 aacgtctgct ccattcctcc gctttgcggt ttgaagcttg cctcatagcg catactggtg
    37801 cctgccggag tcacttcctg ctggcgcgaa cggcgatcgt taatcggttt ttcccgcttg
    37861 ttgctcacca ccaccagctt ttgctggagc ggagccatat cattatcagc tttttcccgt
    37921 cgctgttgca taaaacgaaa cgatgcggcg acgacaatta agccaatgat aacaataaag
    37981 aaaagaggtg gtttactcat ctttatccct catcggaaaa tgcggaatta agcataccct
    38041 gccagttatg gtgttgtcat ccgtccaccc tcgccactaa actggaagca agaccgtagg
    38101 cattctgctt acgaaaaaat aacgaattca aggaactaag atgctttggt cgtttatcgc
    38161 tgtctgtctt tccgcatggc tatctgtgga tgcatcgtat cgtgggccaa cctggcaacg
    38221 ctgggtgttt aaaccgttaa cccttcttct cctgctgtta ctggcctggc aagcaccgat
    38281 gttcgacgcc attagctatc tggtgctggc gggactgtgc gcctcactgc tgggcgatgc
    38341 gctaaccctg ttgccacgtc aacgtctgat gtacgccatc ggcgcgtttt tcctctcgca
    38401 cctgctgtac accatctatt tcgccagtca gatgacgctc tctttcttct ggcctctgcc
    38461 actggtgttg ctggttctgg gtgcgctgct actggcgatt atctggacgc ggctggaaga
    38521 gtaccgttgg cctatctgca cgtttatcgg catgacgctg gtgatggtgt ggctggcagg
    38581 tgaactgtgg ttcttccgtc cgaccgctcc ggcgctctct gcgtttgttg gcgcttcgtt
    38641 gctgtttatc agtaactttg tctggctggg gagccactat cgccgacgct tccgtgcgga
    38701 taacgcgatt gctgcggcct gctactttgc cggtcacttc ctcatcgtcc gctcactgta
    38761 tctctgataa aacttgactc tggagtcgac tccagagtgt atccttcggt taatgagaaa
    38821 aaacttaacc ggaggatgcc atgtcgacac ctgacaatca cggcaagaaa gcccctcaat
    38881 ttgctgcgtt caaaccgcta accacggtac agaacgccaa cgactgttgc tgcgacggcg
    38941 catgctccag ctcgccaacg ctctctgaaa acgtctccgg cacccgctat agctggaaag
    39001 tcagcggcat ggactgcgcc gcctgtgcgc gcaaggtaga aaatgccgtg cgccagcttg
    39061 caggcgtgaa tcaggtgcag gtgttgttcg ccaccgaaaa actggtggtt gatgccgaca
    39121 atgacatccg tgcccaggtt gaatctgcgg tgcaaaaagc gggctattcc ctgcgcgatg
    39181 agcaggccgc cgacgagcca caagcatcgc gcctgaaaga gaatctgccg ctgattacgc
    39241 ttatcgtgat gatggcaatc agctggggtc tggagcagtt caatcatccg ttcgggcaac
    39301 tggcgtttat cgcgaccacg ctagttgggc tgtacccgat tgctcgtcag gcattacggc
    39361 tgatcaaatc cggcagctac ttcgccattg aaaccttaat gagcgtagcc gctattggtg
    39421 cactgtttat tggcgcaacg gctgaagctg cgatggtgtt gctgctgttt ttgattggtg
    39481 aacgactgga aggctgggcc gccagccgcg cgcgtcaggg cgttagcgcg ttaatggcgc
    39541 tgaaaccaga aaccgccacg cgcctgcgta acggtgagcg ggaagaggtg gcgattaaca
    39601 gcctgcggcc tggcgatgtg attgaagtcg ccgcaggtgg gcgtttgcct gccgacggta
    39661 aactgctctc accgtttgcc agttttgatg aaagcgccct aaccggagaa tccattccgg
    39721 tggagcgcgc gacgggcgat aaagttcctg caggagccac cagcgtagac cgtctggtaa
    39781 cgctggaagt gctgtcagaa ccgggtgcca gcgccattga ccggattctg aaactgatcg
    39841 aagaagccga agagcgccgc gcccccattg agcggtttat cgaccgtttc agccgtattt
    39901 atacgccagc gattatggcc gtcgccctgc tggtaacgct agtgccgccg ctgctgtttg
    39961 ccgccagctg gcaggagtgg atttataaag gtctgacgct gctgctgatt ggttgcccgt
    40021 gtgcgttagt tatctcaacg cctgcggcga taacctccgg gctggcagca gcagcgcgtc
    40081 gtggggcgtt aattaaaggc ggtgcggcgc tggaacaact aggtcgtgtt acccaagtgg
    40141 cgtttgataa aaccggtacg ctgaccgtcg gtaaaccgcg cgttaccgcg attcatccgg
    40201 caacgggtat tagtgaatct gaactgctga cactggcggc ggcggtcgag caaggcgcga
    40261 cgcatccact ggcgcaagcc atcgtacgcg aagcacaggt tgctgaactc gccattccca
    40321 ccgccgaatc acagcgggca ctggtcgggt ctggcattga agcgcaggtt aatggtgagc
    40381 gcgtgttgat atgcgctgcc gggaaacatc ccgctgatgc atttgctggt ttgattaatg
    40441 aactggaaag cgccgggcaa acggttgtgc tggtagtacg taatgatgac gtgctgggta
    40501 tcattgcatt gcaggatacc ctgcgcgccg acgctacaac tgccatcagt gaactgaacg
    40561 cgctgggggt taaaggagtg atcctcaccg gcgataatcc acgcgcagcg gcggcaattg
    40621 ccggggagct ggggctggag tttaaagcgg gcctattgcc ggaagataaa gtcaaagcgg
    40681 tgaccaagct gaatcaacat gcgcccctgg cgatggtcgg tgatggtatt aacgacgcgc
    40741 cagcgatgaa agctgccgcc atcgggattg ctatgggtag cggcacagac gtggcgctgg
    40801 aaaccgccga cgcagcatta acccataacc acctgcgcgg cctggtgcaa atgattgaac
    40861 tggcacgcgc cactcacgcc aatatccgcc agaacatcac cattgcgctg ggactgaaag
    40921 ggatcttcct cgtcaccacg cttttaggga tgaccgggtt gtggctggca gtgctggcag
    40981 atacgggtgc gacggtgctg gtgacggcga atgcgttaag gttgttgcgc aggagataag
    41041 gcaaaccgat cgcaacattg agcgcgatcg gtcccctcgc ccctctgggg agagggttag
    41101 ggggtgagga aaaggcggca tcgaagccaa tcagccccta tcaaccgcct ttacgaatca
    41161 aataacgata aggcagtcca tccgtctctt tcgcaaccag ttcgtgttcc ataaaggtac
    41221 aaaacccagg aatatcgcgg gtagtggccg gatcgtcggc gataatcagc aacgtttcgc
    41281 caggctgcat attgcgcacg gttttgcgca ccatcatcac cggttccggg cagcgcaggc
    41341 caagcgcgtc gagtgtgtgg tcagggctgg aaaagagatc ggtcattttc ttctcatcac
    41401 ttaaaaaaac ggcgctagtt tacgccctgt gagtccgtaa gcaaaccagg ttaacgattg
    41461 cgtgaaaatt aaccattgca ttgtcaacgt aaagcagtat catgcggcgg ctcgaaaaaa
    41521 gggtaagcac gttattatgt taaggtaaca gacgtgtcgt acgtattggg ttccctcacc
    41581 ccaatggtta atcaaaaagg tacaatatga acgttttctc gcaaactcaa cgctataagg
    41641 cgttgttctg gttatcgtta tttcatcttc tggtgatcac ctccagtaac tatctggttc
    41701 agcttcccgt ctccattttt ggtttccata ccacctgggg cgcgtttagc tttccgttta
    41761 tttttcttgc taccgacctg accgtgcgta tttttggcgc accgctggcc cgacgcatta
    41821 tcttcgcggt aatgatccct gcgttattaa tctcctacgt catctcgtcg ctattctata
    41881 tgggttcctg gcagggattc ggcgcactcg cccacttcaa cctgtttgtc gcccgtatcg
    41941 ccaccgccag tttcatggcc tacgcgctgg ggcaaatcct cgacgtgcac gtttttaacc
    42001 gcctgcgtca gagtcgccgc tggtggctgg caccgacagc gtccacactg ttcggtaacg
    42061 tcagcgacac gctggccttt ttcttcattg ccttctggcg tagcccggat gcctttatgg
    42121 ctgaacactg gatggaaatc gcgctggtcg attactgttt caaagtgtta atcagtatcg
    42181 ttttcttcct gccaatgtat ggcgtattac tcaatatgct gttgaaaaga ctggcagata
    42241 aatccgaaat caacgctttg caggcgagtt aaaggttcgt tatcagagtt gtgataagat
    42301 ggatgaatga gccgttatgg ccgtttatcg aaaggaagaa gtcaatgcgc aatctggtta
    42361 aatatgtcgg aattggcctg ctggttatgg ggcttgcggc ctgtgatgat aaagacacta
    42421 acgctacggc gcagggttcg gttgcggaaa gtaacgctac cgggaatccc gtcaacctgc
    42481 ttgatggcaa gttaagtttc tcgctgccag cggatatgac cgaccagagc ggtaagctgg
    42541 gaacgcaggc caataacatg catgtctggt ccgacgccac cgggcagaaa gcagtcatcg
    42601 tcatcatggg cgatgatccg aaagaagatc tggcggtgtt ggcgaagcgt ctggaagatc
    42661 agcaacgtag ccgcgatccg cagctgcaag tggtaaccaa taaagccatt gagctgaaag
    42721 gtcacaaaat gcagcagtta gacagtatta tctccgcgaa aggccagacg gcgtactctt
    42781 ccgttattct gggtaacgtg ggtaatcaac tgctgaccat gcaaattacg ctgccagctg
    42841 acgatcagca aaaagcgcaa accaccgcag aaaacatcat taatacgctg gttattcagt
    42901 aagttttaag atgatgaggc ggcctcaggg acgtgttccg gaggccgttt ttttaatcgc
    42961 cacgtcagta ataacgcgat tgcgaccagt cccgccgccg ccagataaat caccggtacg
    43021 cccgcccagc tcatcaccag cccagccagt ggtccagtca cgccaagcga taaatccata
    43081 aatacggtgt aagttgccag cgccgccccc tgattttgct gcggaaccgc ttttaccgcc
    43141 actacaccca atgccgggaa caccagcgaa aacccggccc ccgccagtaa gacgccgatt
    43201 ttcgccatcc acggcatagt cgccacgcca accagtagca ggccgattat ctcaacgcta
    43261 aagcaaatca tcgctacgtt taagccaccg atacggttaa tgccgttagg gaataacaaa
    43321 cgcgtaccga caaacgcaca gctaaacagc gtcagcgcga aagccgcacc gtcccaacct
    43381 ttagcgtcat aaaacagcgt gataaaggtg gcgatgacgc caaatccggc ggaagccagt
    43441 gccagcgcca taccgtacag ccagacgcgc ccaagcaccg cgcgaaacgg cagcggtttg
    43501 cctttactgg cttttaccgt cggacgcggg atcgccaaca aaatggccac cagcgccacg
    43561 cccataatga ttaacgctaa cgcctgcaag ccgccccagt gataaaacac gacgcctaac
    43621 ggcgcaccca tcgccatcgc cccgtaagtg acaatgccgt tccacgaaat cacccgcccg
    43681 atatgcagcg agccaaccac tccaacgccc catagggtcg atcccgttcc ggcaaaactt
    43741 tgcccaatcc caaggatgac gcgccccagg caaagtaata acaggctgat gacaggcaga
    43801 ctggcggtta atcctgccgt cagatacccc agaccgctca aaaagcagcc gcataaaccg
    43861 aagacgacaa tctttttggg tcccagcaaa tcggcgtaac gtccggcatg agggcggctc
    43921 agcaaggtgg cgaaatattg caggctgata accaatcctg cccagaaggc gctaaagccc
    43981 atcacatcat ggacatagcc cggtaataca gcgagcggca acccgatggt gaggtagctg
    44041 gcgaagttaa acatgacaat ggagacaatg cgcaaattca ggcgcaatcc gtttagcgcg
    44101 ggttcggcta cgggttcggg catgaggatc accacatttt tacaacagtg tttcattttt
    44161 accatgtgct gacgtgaaaa tcagcagtaa gaatcagaat attgctggcg tgactcccgc
    44221 gctacactta acacaaaaag tcacaaggaa gccccaatgg aaacccctca acccgataaa
    44281 acgggcatgc acattctgct caagttggcc tcgctggtag tgatcctcgc gggcattcac
    44341 gcagcggcag atatcattgt gcagctgtta ctggcgctgt tttttgccat cgtcctcaac
    44401 ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc ccgttgccat tacgattgta
    44461 gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg tactggcggc atcgtttaac
    44521 gaatttatct ctatgctgcc gaagtttaat aaggagctga cgcgcaaact ttttaaattg
    44581 caggagatgt tgccttttct taatttgcat atgtcgccgg agcgaatgct gcagcggatg
    44641 gactcggaaa aagtggttac cttcaccaca gcgctaatga ccgggctttc cggggcaatg
    44701 gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc tgtttgaagt gcgccacgtc
    44761 ccttacaaaa tgcgttttgc gctgaataac ccacagattc acatcgcagg attacaccgc
    44821 gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc tactcagttt atggacaggt
    44881 gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt ttgcgctgat gtgggcagta
    44941 ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg taatttccgc cgtaccgcca
    45001 atgattcagg tgctgctgtt taatggtgtt tacgaatgta ttctggtcgg cgcattgttt
    45061 ttagtggtcc atatggtcat cggcaatatt ttagaaccac ggatgatggg ccatcgcctg
    45121 gggatgtcca ccatggtggt atttctttca ttgttaatct ggggatggct gctcggcccg
    45181 gtagggatgc tactttcggt gccattaacc agcgtgtgta aaatctggat ggaaacgacg
    45241 aaaggtggca gcaaactggc gattttactg gggccgggca gaccgaaaag tcggttaccg
    45301 ggatgaggcg acaagtgata cgatacgcac tttcattttc cattaaacgt tggccctgat
    45361 atgtatcgga tagttctggg gaaagtttcg accttaagcg cagctccact gccaccgggt
    45421 ttacgcgagc aagcaccgca aggtccacga cgcgaacgct ggctggcggg gcgtacattg
    45481 ctttcgcaca cgctttcccc gctaccggag atcatctatg gcgaacaagg caaacctgca
    45541 ttcgcgccgg aaacgccgct atggttcaac ttaagccata gcggtgacga tatcgctctg
    45601 ctgttgagcg atgaaggcga agtcggctgc gatatcgaag tgattcgccc gcgcgtcaac
    45661 tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac acgctgaaat ggacgccgtg
    45721 catcctgatc agcaactgga aatgttctgg cgcatctgga cgcgcaaaga agccatcgtt
    45781 aaacagcgtg gcggcagcgc ctggcaaatc gtcagcgtag atagcaccta tcactcctcg
    45841 ctgtcagtca gccattgtca gctagaaaat ttaagccttg cgatctgcac ccccaccccc
    45901 tttacgctca ccgccgacag tgtgcaatgg atcgattcag ttaactgatc cgcccacccg
    45961 actgcccatc tattgatcca gaacaggtaa tcagtatgac gaatacttaa aatcgtcata
    46021 cttatttccg ccatctattt taatccattg gggttaccat gctctccaca ctccgccgca
    46081 ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca tgccgctgca ccagatgaaa
    46141 tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc gcacctttac acgcctaacc
    46201 agatgttcgc ccagagcatg gtttatgaac cattggtgaa atatcaggca gacggttcgg
    46261 tgatcccgtg gctggcaaaa agctggactc attcagaaga tggtaaaacc tggaccttca
    46321 ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt cgatgccgag gcggcggcag
    46381 aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc ctggctggag ctggcaaacc
    46441 agattgttga tgttaaagca ctcaataaaa cagagctgca aattaccctg aaaagcgcct
    46501 actatccttt cctgcaagaa ctggccctgc cccgtccttt ccgttttatc gctccctcgc
    46561 agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc gattggcacc ggaccgtgga
    46621 ttttgcagga atcgaaacta aatcagtacg atgtcttcgt ccgtaacgaa aactactggg
    46681 gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat cccggacccg actacccgcg
    46741 cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg aaacgaaggg ttattaccgc
    46801 tcgatacctt cgcccgcttt agccagaatc cggcttacca cacccaactg tcacagccga
    46861 tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc caccaacgag ctggcagtac
    46921 gtgaagctct taattacgcg gtaaacaaaa aatcgctgat tgataacgcg ttatatggca
    46981 cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc ttacgctaat ctcggcctga
    47041 aaccgcgcca gtacgatccg cagaaagcga aagagttgct ggaaaaagcc ggttggacgc
    47101 tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc gctgcgcatt gaactgtcgt
    47161 tcatcggcac cgatgcgtta agcaaatcga tggcggaaat cattcaggct gatatgcgcc
    47221 agattggcgc agatgtctcg ctgattggcg aagaagagag cagtatctat gctcgtcagc
    47281 gcgacggtcg ttttggcatg attttccacc gcacctgggg cgcgccatac gatccacacg
    47341 ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt ccaggcacag caaggattag
    47401 ccgacaagcc gctgattgat aaagagatcg gcgaagtgct ggcgacccat gacgaaacgc
    47461 aacgtcaggc gctgtatcgc gacattctga cccgtctgca tgacgaggcg gtttatctgc
    47521 ctatcagcta catctcgatg atggtggtat caaaaccgga gctgggtaat atcccctacg
    47581 cgccgatcgc caccgaaatt ccgttcgaac agattaaacc ggtgaaacct taatgttgcg
    47641 ttacgtatta cgccgctttc tgctgctgat cccgatggtg ctcgccgcct cggtgatcat
    47701 ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc gattatctgc gtttatctaa
    47761 cctgccgccg acgccggaga tgctggcctc tacccgcacc atgctcgggc tggatcagcc
    47821 gctgtacgtc cagtacggta cctggttgtg gaaggcgctg catcttgact ttggtatctc
    47881 attcgccagc caacgcccgg tactggacga tatgctgaac ttcctgcccg ccacgctgga
    47941 acttgcaggt gcggcgctgg tattaattct gctcacttcc gtaccgctcg gtatctgggc
    48001 ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc tttatcgcgt ttcttggcgt
    48061 gtcgatgccc aacttctggc tggcattttt gctagtgatg gcgttttcgg tgtatctgca
    48121 atggctaccc gcgatgggct acggcagctg gcagcacatc attttacctg cggtttccat
    48181 cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc gccagtatgc tggacgtcgc
    48241 cggtcagcgt cacgtcacct gggcgcggct acgcggcctg aatgacaaac agaccgaacg
    48301 ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc gccgtgggga tgcacattgg
    48361 cgaattgatt ggcgggacga tgattatcga aaacatcttt gcctggcctg gcgtcgggcg
    48421 ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg attcagtgct ttacgctgat
    48481 gatggtggtg gtttttgtgg tctgtaattt gattgtcgat ttgctcaacg ccgcgctgga
    48541 cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact ttttcctctc ttcccgctgg
    48601 tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc tgattgcgct caccagccag
    48661 tggtggcagc cgtacgatcc acaggcaatt gatttgccgt cgtgcctgct ttcgccggat
    48721 gcgcagcact ggctgggcac cgatcactta ggtcgcgata ttttctcgcg gctgatggca
    48781 gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc tgctggtgct gacattaggg
    48841 ctggttattg gcggcagcgc cgggttgatt ggcgggcacg ttgatcaggc caccatgcgc
    48901 gtcgccgata tgtttatgac cttcccgacc tcgattctgt cgttctttat ggttggcgtg
    48961 ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc tgtcgcactg ggcgtggtat
    49021 gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac gcgagtttgt gctggcgtca
    49081 cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc atctggcagg cgcggtgatc
    49141 ccttcgctgc tggtgctggc aacgctggat atcggccata tgatgctgca cgtcgcgggg
    49201 atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg aatggggcgt gatgattaac
    49261 gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt tctggccggg gctggcgctg
    49321 tttatcagcg tgatggcctt taacctggtg ggtgacgcac tgcgcgatca tctggatcct
    49381 catctggtga cggagcacgc acactaatgc cgcaacagat tgaactacgt aatatcacgc
    49441 tacaggccgc gcagccgctg gtacacggtg tatcgttaac cctgcaacgc gggcgcgtgc
    49501 tggcgttagt cggcggtagc ggcagcggga aatcattaac ctgcgccgcg acgctgggca
    49561 ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt agccgatggc aaaccggttt
    49621 cgccttacgc cctgcgcggc atcaaaattg ccaccatcat gcagaacccg cgcagcgcct
    49681 ttaatccact gcacaccatg cacacccacg cgcgggaaac ctgcctggcg ttagggaaac
    49741 ccgccgatga cgctacgctt accgctgcca tagaagcggt ggggctggaa aacgccgcgc
    49801 gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat gttgcagcgc atgatgattg
    49861 cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga tgaaccgacc accgacctcg
    49921 acgtggtagc acaggcgcgc atcctcgatc tgctggaaag cattatgcaa aaacaagcgc
    49981 cgggaatgct gctggtcacc catgatatgg gcgttgtggc gcgtctggcg gatgacgtgg
    50041 cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt agaaacgctg tttaacgccc
    50101 ccaaacatac ggtgacgcgc agcctggttt ccgctcatct cgccctctac ggtatggagc
    50161 tggcatcatg accttactta acgtctgcgg cctttcccat cactatgccc acggtggatt
    50221 tagcggaaaa catcaacatc aggcggtgct gaataacgtt tccctgaccc tgaaaagcgg
    50281 cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa agcaccctcg cgcggctgct
    50341 ggtaggctta gagtctccct cacaagggaa tattagctgg cacggcgaat cgctggcgaa
    50401 acttaatcgc gcccaacgta aagccttccg ccgtgatatt cagatggtgt ttcaggactc
    50461 catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc ctgcgtgaac cgatgcgcca
    50521 cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc agcgaaatgc tgaaggcggt
    50581 cgatctcgat gacagcgttc tcgacaaacg cccaccgcag ttaagcggcg gccagctcca
    50641 gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa ttgctgattc tggatgaagc
    50701 cgtttctaac cttgatctcg tgttacaggc gggcgtcatt cgcctgctga aaaagctaca
    50761 acaacagttt ggcactgcct gcctgttcat cactcatgac ttacgcctgg tggaacgctt
    50821 ttgccagcga gtgatggtga tggacaacgg acaaatcgcc gaaacccagg cggtgggaga
    50881 taaattaacc ttttcctctg acgccggacg tgtgctacaa aacgcggtat tacccgcatt
    50941 ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa tgcaacgagt caccatcacg
    51001 cttgatgacg atttactgga gacgctggac agcctgagcc agcgtcgtgg ttataacaac
    51061 cgttccgaag ctatccgcga tattctgcgt agtgccctgg cgcaggaagc tacccagcag
    51121 cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg aacacgaaaa acgcgactta
    51181 gccagccgta ttgtttccac ccagcatcat caccacgacc tctccgtcgc cacgctacat
    51241 gtgcacatca accacgacga ctgtctggaa atcgccgtgt tgaaaggtga catgggtgac
    51301 gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg tgcggcacgg gcatttgcag
    51361 tgcctgccga aggaagattg agttatttct aaaacaagga aagaaaagat attaactgaa
    51421 cttaatttat aaaattaagt tcagttgcat agatagatcc tgtaaaggca gatattctgc
    51481 gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct cattcagtgt ttcaataata
    51541 taggtattaa cagagacttg ttgctgcgca gccgcattat taaggcgttc actcagcgat
    51601 tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac gcgcatacgg ctcaataccc
    51661 gcagctttac aatcctcaag atactctcgc agggaaagct ctccttcttt ttgcagcccc
    51721 tgaatactgt cagagacaaa atcacaataa cctgacaagc ccaggaattt gccacgaaat
    51781 gcattcagtt ctggcacgta ggtaataaca gctggttgcc cggcaatctc catagagttt
    51841 ggcgtcttca gtttgatcat ggttttaccc ctatacttag taaccagtca cacacgcttt
    51901 ctacagcgcc tttatcggta tctggcgacg gatgaggccg atggaaacac gcaacgctca
    51961 tatttagtat gaacttgcaa cgcgaacctc ttccttcctt aatttctccg cctaatgctt
    52021 taaccagtga ctctatatca gaccacttga tcccttgagg aacaggagtt ttaaatattt
    52081 gatccaatgt atttttttgt ttcttccgta aagcctgtac cttggttctc atggcatgtc
    52141 cttatgactt cactttatga catcacagta tgaagtcaag tttcccttcc ttacgccatt
    52201 gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga aaaacgcgcc gccaattgcc
    52261 atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg caccccggta gaggatggcc
    52321 tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca taatgtcctg caccatctgc
    52381 ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt gcagcggcag cagcaccaga
    52441 atcaccagca gccccagttg cggcattgaa cgcgctatcg tccccataaa aatgccgatt
    52501 gacgtggtgg caaacagact gagcgccacg cccagcataa acagcgggat cgagccttca
    52561 atcggtacgc ccagtacacc tttcaccatc agcaccagcg ataatcccga taccaccagc
    52621 accaccagcc ccatcgacca gatcttcgcc atcatgatct caaacggcgt tatcggcatc
    52681 accagtaaat gttccaccgt gccgtgttca cgctcgcgga tcagcgccga tccggtcaat
    52741 acaatcgcca gcatggtaat gttgttgatg atcgccatca ccccgccaaa ccacgcggga
    52801 tcaaggttcg ggttaaagcg catccgggtt tccagcgata ccaacggttc gctgttatca
    52861 cggtagcgcg cgacaaagct gttcacttca ccgttgataa tattctggat atacccattg
    52921 ccggtaaatg cctggctcat gcgcgtggca tcgacgttca cctgaatatc cggctggcgt
    52981 ccggcgagga catcacgctg aaaattaggc ggaatattta tcgcgaaggt atagcgtccg
    53041 gcgtccagtc cggcatccat ctcatcggcg gtgatcatct ccggtggcaa aaaccacgga
    53101 cgatagaagc tgttaacgat ccggttcgat aactgcgatt gatccatatc ggcaatggcg
    53161 atcggcgcga ggttcaacga tcctggcgta acggtcgctg acgaatacac cgacaccgta
    53221 aacgagaaga caatcagcgt cagcatcgct ttatcaccga gcagactgcg caactctttg
    53281 atacccagat taaaaatatt gcgtaaatgg cgcatcatcc ctcctgtttt ttcagcagca
    53341 ggatgcttaa gcccatcacc agcgggatgg ctatcagcaa cgggataaaa agttgccaca
    53401 aatcagtcag atccagcgct ttcgagaacg ttccgcgggc gatagtcaga aaatgactgg
    53461 tcgggtaaac ctcgccgatc caacgtccag gcccttccag cgaagctacc ggatcgatca
    53521 tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc cgttccgaaa atggcggcaa
    53581 tctggctttt cataaaggtg gagatcagca gccccattcc agtggcaatg atgatataca
    53641 gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg cggtacgcca aacacaaaca
    53701 ccgacaggcc gcagagcagg aaaaagttca gcatccccag cgcgatgtac ggcagctgtt
    53761 tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta aaggttgata atcgacccta
    53821 gctctttttc ccgcaccacg ctaagggcgc ttagcattga cgggatcatc atcagcagaa
    53881 gcgggatcac cgccggaaca atcgctggca ggctttttac gtccgggtta tagcgataac
    53941 gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt cgattgtcgg ctcgccacat
    54001 cctgaagcca actctggtgc attgcctgca cgtaaccttt taccgtttcg gcacgactgg
    54061 gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt accacgcgcg atatcgcgcc
    54121 cgaaattggg cgggatctca atggccaccg tgatatcgcc cgcacgcatc cgacgatcaa
    54181 gctcgtcata actggtgagc ggcggctgtt cgataaagta acgggaaccg gagaggttga
    54241 gtgtccacgc ctggctactg acggtctggt cgcggtcgag caccgcaaag cgcaggtttt
    54301 ccacatccat actgatgccg taacccatta tcagcatcag gatcaccgtt cccatcagcg
    54361 ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc ttcgcggcgg ctgtagctaa
    54421 acagacggcg caggctaaat ccctgacgcg gcgcgtgggt ggtgtcgtgt accacgggcg
    54481 gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa ataggcgata aatgcctctt
    54541 ccagactggc ggctccgcgt ttctcaacca gttcctgcgg tgtaccgctg gcaagcactt
    54601 ttccggcgtg catcagtgag atgcggtcgc aacgttccgc ttcgttcata aagtgggtgg
    54661 agatgaagat agtcactttg tcctggcgcg agagatcgac catcaactgc cagaacatat
    54721 ccctcgccac cggatcgaca ccagaagtag gctcatcgag gattaacatc tccgggcgat
    54781 gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc gagcggcaat gactccggca
    54841 gaacatcttc aacgtcgttg agcttaaaac gctcgctcat ttcagccact cgcgcgggaa
    54901 tttccgcttc cgggatgtga aacaaacggg catgtaactc caggttttgc cgcacggtga
    54961 gttcgttata gagcgaaaac gcctgcgaca tatagcccac acgacggcgg gtatcgatat
    55021 cttttggatc aaccggttgc ccaaatagcc acgcctcacc ttcgctggcg ggcagcagtc
    55081 cggtaagcat tttcatggtg gtggatttac cgcagccgtt cgaaccaagg aatccaaaaa
    55141 tctccccgcg tggaatgcgg aaattgacgt gatcaacggc aacgaaggaa ccaaaacgca
    55201 tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc aggttgatac ggtgggatca
    55261 ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag attaataaat gcttcttcca
    55321 gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct gccggttgcc agcacttctc
    55381 cggcattcat cgctaccagc cagtcgaagc gttcggcctc ttccatatag gcggtggcga
    55441 ccagcacgct catattgctc tgccgctggc gaatactgtc gatcagatcc cagaactggg
    55501 cgcgggagag cgggtcaacc cccgttgttg gctcatcaag gatcaacagt tccgggtcgt
    55561 ggattaacgc gcagcacagc ccaagttttt gcttcatccc gccggagagt ttacctgccg
    55621 gacgatcgcg aaacggtgct aacccggtgc tggtcagcag ctcattaatt cgcgcttccc
    55681 gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc gacattttca tacaccgaca
    55741 aggtgtggta gaggtttttg cccagcccct gcggcatcca ggcgatgcgc gggcagacgt
    55801 ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac caccacattg ccctgctcaa
    55861 tgacgcgggc accggaaatc agtgacaata aactcgactt cccgacgcca tccggaccaa
    55921 tcagcccgac catgcaacgg gccggaatat cgagagtgat attgttcagc gcaacggttt
    55981 ttccataatg ctggctcacg cccgccagtt gcgcgacagg cgggacggga accagttcca
    56041 gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc cacggaagtt cttcattcac
    56101 ccgcacccac gctacgcccg gtaaaccggt tttgacatat tccagatgct gctggagtaa
    56161 ttccggtggg atacgcgctt tgacgcggaa catcagtttc agccgttcat cgctggtttc
    56221 gacggttttt ggcgtgaact gggcgacact ggcgacaaaa ctgatggttg caggaatacg
    56281 cagatctggc gcggcatcga ggatcagccg ggcttcaccg cccagtttca gcgtgcccgc
    56341 ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg agatcgacca tattcagcac
    56401 ccgaccgcct gccgccagca cttcccctgg ctcggcaacc cgatactgca cgcgtccgtc
    56461 acgcggggct ttcagttcac tgtcatcgat atctgcggca atgcgccgtt cagtggcttg
    56521 cgctgcttcg acccgagttt gcgcctgaat gatattggtg cgtgctgctt ctatagccgc
    56581 tttagaagcc gaaacctggg ctttcgctga ttccagcgca gctcgggcgc tctcagcggc
    56641 ggcgcggtcg tcatccagct gttgcgcaga aatagcccct cgttgggcga gcgagcggga
    56701 acgcgtatga cgtttggcaa cagagtccag ttcggcctgg cgttgattga ccagcgactg
    56761 tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc tgcgcggcgg caacggcgct
    56821 ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt tcctgcaaca cgcgagtatc
    56881 catcttcgcc agcacttcgc cttcgcgaac aaactggcct tctttcacca gaatggtgtc
    56941 gatacgcccg gcaattttgc tggcgatatc cacttccgtc gcttcaatgc gcccattgct
    57001 gacagcaaag ccttccggca cacccgccgg gcgcaacagc caccacgcca cgacggcagc
    57061 caccgccagt gccccgacaa cccaccacgc cagatggcgc ttactcttat ccataatcga
    57121 cccgccataa tccctgtaag caaacgacgc gtgttacaac tgccgccgaa cgacgttcag
    57181 cggattcttc catccgggat aagaaggaaa cgacaaatac gaccagcgcg cagagcattg
    57241 agcgccaacg gggaaggctt agtgaaaacg ggtagtaatg ttgtcatcat cagcattcct
    57301 ggccgtaaat gaaacacgca tactcataag tgtgatctat aaaactggct gacaaaacgg
    57361 tatccatcct gttggatcgc atttcacgcc tgttcagttg caggaacaga gcattccttt
    57421 ttacgtttta tcaacagtct caaccagata gtaacattaa tattcatgtt gtctatggtt
    57481 cagagctgta acattgtgcg ccttctaatc aaaggcataa gtccatttct gtttttttca
    57541 tgctggtaag acttccaggg agtggttatc tctggccctg caccagggtt aatttggcaa
    57601 ttatgcatgc tgaacattct tccagcatgt ttactgtcac gggcgttaag acctaaaact
    57661 gtccccgttt cacctatgcg ttctctccca ccatttccgt tatgtgcaac aacttgattt
    57721 atacctaaca tttgtgaaca tgctaatagt gcttcaagtt taggtctgta atctgtcgaa
    57781 acagaaaaat aacgattaaa ggatcgacaa gagatagccc ggctagtttt aaactttttg
    57841 ccattaatta tagcatgatg ctttttatta ataatttccg ctaattcgac cggatcgaga
    57901 gcattggtca tttcatcaat agagacagtt atcggtccta taatgtaacg ttttcgctga
    57961 tcatcaaaag ttatcccatg atgatttgcc attattttgt atttggggtc atagaaacat
    58021 acaggataat cttttatccc gtcgtaggta tcctcatcaa gtggtttcat gcgcgtgaag
    58081 ttttgcatat agttaccatt gcaattcgtt tcatgatttc cagctaatac atgaacattt
    58141 tcattgatac tcattatgcg ttttaacaaa tcgatgataa attgatcgcc acagatatta
    58201 ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg gcgtgactgc gtctttatta
    58261 ataaccaaat aaggctgaag actttctaaa acattatctc ttctgttcag tatactgatt
    58321 attgtattaa cattagtgtc aagttgctca ttaaagttct cccttagcaa tacgttgaga
    58381 acttcatctt ctatattaag caactggcta aggaaatttt ctccttcagc catgattccc
    58441 aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga ctgagccatc ggtatcgcca
    58501 aaataggtcg gcctggtact tgtgggatca acaggtgaac aattggagaa ataggttctg
    58561 tcagcgaact ggggttttat aattgtttcg aatttaatat caaatacatt gtattgcgat
    58621 atttcccagt tccagatggg tttgtacgac tcagtaactg caacatcatt agcattatca
    58681 tttactgtca tattattaag taaataaaca atgctgcacc aattaatatc ggtattatca
    58741 agagggcaac cgtaattttt actattaatt gtcacaataa actgataaca gttattccta
    58801 cgtagaatat gagcgcgcat gcccgatagc atactatatt tttctgaatg attcgtttgt
    58861 atggtaaatg aaattttgtc atcttgaata gattgttgga aacgttctat ttgccaatca
    58921 tcaatttttt ccacaacttc accgagcatt gtcctggcaa tacttgatat atcatcacct
    58981 ataccatttg caatataccc aaacagttct ttagtgaagt tggtaacatg ctcttgcacc
    59041 atatcatttg ttgcgattgt cgttgtactg ccattagtgc ctgccacagt tccaattttc
    59101 atcttttacc tcatattatt caccgcgagg aatacatagt taacatttta tcttatgaca
    59161 tacacctcat attcgatggg gaaataatta tattttgcac aaatcattca gccttaaagc
    59221 ataatatgaa atttcttatc tcatagcaga gaaagtccct aaatattgta gaggcgtcat
    59281 cagtacatac ctctacaatc gattaagaaa acctatgaaa tactggcaaa attaaaaaga
    59341 gataccacag cactcatgaa gttttattga ggttgaaaac aactaagccc tgggacacct
    59401 tttttatcac cacgttcgtt aggtgctatc ttccaagaat ttagccccat ggtataatta
    59461 tcaatttttt gatgctggtc tacccctaat ccataatggc caaatttctg tttagttttt
    59521 ttgagcgtct gtccacattt gaagatatct tcaggttttg gtctgaaagc agtagtgtta
    59581 ttaaaataat tttcatatac aggtgtgggt gctatagcat tgcttctaat tagatggaga
    59641 agatgatcat ccatgtgttg tttatgtttt ttattacata tatttgctaa ttcctcagga
    59701 tcaattgggt ctttcatctg attaaatgga acctgcaaag caccgagaca ataacattga
    59761 ttttcttcat ttcttattat tccgtgatga ctggttaaaa ctttagtttc tgagttataa
    59821 ttacaaacat cgagcgtttt aattaagtta tacgtatcac cggcagagag tttataattt
    59881 tctagtcgtg cagtatagtt accattaaaa ttgatttcat gatttccagc cagaacgatg
    59941 acatttttat taatacggct atctttatta ccctccatat ttcgcataga atttagcaaa
    60001 gtaaggatat atttatcacc aaaaatagta ctaaatctat cgcctgtatg atccccaagg
    60061 aagatacaag gggttatcgc atcgtccttt aaaaaaacat gttgatgcaa ctcattcaat
    60121 atcatgtctc tcgtatttaa taaattgtag agcaatttat tatttttatg tatgtatgca
    60181 gatgaagcca tgtcttcgtg ttttaataat tcacataata aactccaacc cttttccctg
    60241 atgcctatat gccctgaaac gagcaatgaa taaagtacag cgcctactct tccatctgta
    60301 tcgccaaaat aagccgtgtt ggcattttgt gggttaatca caccacattg tttataaaag
    60361 tctattttag aaaacacaga tttaacatag ctatcaaact tatccttagg aaacgtctct
    60421 ttagagacat cccaacgcca gtcagcttta aaattaccgc taagaagagt aaagaaattt
    60481 tcaggtatga tagaaagccc attaacttta taaattatgt ctgatttatc tccggagaaa
    60541 ttaataaaat aataaccatc cttacactca atcttaactg taatgggaga cgataatttt
    60601 atcacatggt tattgtctgg tataattgta aagataaacg tgttagtagt attatcggca
    60661 aaactattat attcttgaat atctatagat ggctcactat tgtaaaacca tattttctcc
    60721 atctgaagac tatgttcatt taccctccag tgaccatgtt gcaggaaata gatgatatgc
    60781 atgccaaaat cgttatctat ttgcgaatca ttatccagct tatcaatttc aactatcttt
    60841 gaacgatgtg ttttatcaac cttgctatgg tgttgatgat tattaacttt atctgaatgc
    60901 ttatctttat ggtttgaagg gatgtactta aatatattgt caattgcttt cataaataac
    60961 ccgcgatatt tttgacctgt tagtaaaaaa taaatgatac atgtctgaaa gacatattta
    61021 cgacgatata aaacaataat acttatatgt tcgctgattt taagcaccga gactatccgg
    61081 tcttttacta aataatggaa aacaatccat ccctggaaga caattgtaaa aaaaacaaca
    61141 gaataagaat taatcttagg ataaatattt atttatcatg gccttttgac cgctaaaaaa
    61201 tggcactcgg cacagtaaac cccgctatag ttatgctatg catttgtctt taatgatatc
    61261 tcgtcgttat attaacaggc tgaaattatc attaatgcac atttcaatat tagcagggat
    61321 acctgaggag tttaaagtga acatatatat cgggtagctt ttcaaattaa tccctttgat
    61381 tataggctta atttgcatcg cgttaggtgg ctttgtgctg gaaagttcag ggcaacgtca
    61441 cgggtcatgt gctgatttct ctggcggcca tatgcctggc attattcact accgcattta
    61501 ttatcatttc gcaactcacg cgcggcgtta atacgtttta caatacattg ttccccatta
    61561 ttggctatgc ggggtcaatt atcaccatga tatggggttg ggcactgtta gcaggtaatg
    61621 atgtgatggc agacgagttt gttgccggcc atgttatttt tggcgttggt atgattgccg
    61681 catgtgtatc gacggtggca gcgtcgtccg gtcactttct gctcattccc aaaaatgcag
    61741 cggggagcaa gagcgacgga acaccggtac aggcttattc ttcattaatc ggtaattgcc
    61801 tcattgccgt tcccgtttta ctcaccctgc tcggtttcat ttggtctatt acgctgttac
    61861 atagtgccga cataactccg cattatgtcg ccttgggtta accgcaatct gtgcctgtct
    61921 aattggccta gtggccacaa tcgtccatca aacacgtaat acgttctcat ccaaagagca
    61981 ctggctgtgg tgttattggg ttatttttct cggctcagtc acggtgctgc aggggatata
    62041 cgtcttagtc agttccgatg caagcgcccg actggctccc ggcattattc tcatttgcct
    62101 cggaatgatc tgttacagca tattctcaaa agtctggcta ctggcactgg tctggagacg
    62161 tacctgttcg ttagccaaca gaataccgat gataccggta tttacctgtc tgttttgctt
    62221 gtttttgtca tcattcctcg cggaaatcgc acaaactgag ctggactact ttattccatc
    62281 tcgggttctg gttggattag gcgcagtatg ttttacattg ttctctattg tttcaatatt
    62341 agaggccggt tctgcgaaaa aaaattaaaa tttacataat cccagcgtta ttcatatctt
    62401 aacgctggtt ttttacgaac ttagtgaaat aatgtagaat ataaaattat aattttatat
    62461 tggagcacat gtgttaatga tcaaccccgg gatactaaaa atatagacat tattaaactt
    62521 gcttagcctc gcattgcctt tacctgttaa agatgcatcg agaaaattga gtctgaagtt
    62581 ctcatccttt atctgatttt ccagtcccga aactttggcc ttagcattac ctgagatccg
    62641 tccgttacta tctattttgt aggtaaaaaa atacgaatgg gaaaatttcc tcggtgtatc
    62701 ttcatcagtt aagccaacaa tcgtaaaact accgctacca tcttttttta aaatgactga
    62761 aaggttagca gagtagtata aagaatgatc tgttttagca tttcttgtta acgtcgacgt
    62821 acattcaaga gacgttgtgc taccatcacg agccttccag agagcaaaaa aacacacctg
    62881 tagcaatgtg aaaacaagta ttaatataaa atacattttc ttattagtca tataatagca
    62941 cctctgaaca aattgcataa gtttcgtcat tagaggattt tatacatctt gataaatacc
    63001 gctgcccttc cccaagcaaa taatgcatac tatttacatc atatataaaa tttgcacctg
    63061 gcacacaggg taaatgcttt ttcgaaattt cagcggcctc tatttgagat ttagcgatgc
    63121 caatctcgtt aagattctct gaaagatata aatcacaggt gtcaatctta cctgatttaa
    63181 ccaatggata atccgttata ctgtaggtat ctttaaaagc cacggcaaac atttcaataa
    63241 taacaattac taccacaagc gaaatcaaaa tgcgaaaaag aggaatactc cttttgcgct
    63301 ctactttttc aacaacatct tcttgtaaat gagaatcatc ataagattgt ttacttattg
    63361 ttatgttttc actgatgtaa aagcctactt tgggtatcgt aagaatcaca ttttcttcaa
    63421 caccaatatt tttaaaactt ttgcgtaaca gggatatata ttggttcaat gtgttgtttg
    63481 acagatcaaa tgaaaatctc tccaaaacgc tatcaatgat tgagttcctg tcaaccgctc
    63541 caccattact ttccagtaac actgccaaaa tacaccccgc cgtagctgga aaatttatct
    63601 catcctgggt atttacattc gagagcgagc atttgttcgg atcaaaaata atcgcgtcat
    63661 tgataatgaa aaaacatctc cgctccatgc aggcaacaac acaaaataag attttgtcat
    63721 tttagcctat tcctggtgga actgacacaa gaaactaaaa tacaaatata accctgaaaa
    63781 agtcatataa aacttatatc cacagaataa aaaatcaaac atgtcagttt tatcaatata
    63841 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
    63901 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
    63961 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
    64021 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
    64081 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
    64141 tcataaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
    64201 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
    64261 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
    64321 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
    64381 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
    64441 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
    64501 gatatactct ctcctcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
    64561 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
    64621 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
    64681 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
    64741 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
    64801 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
    64861 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
    64921 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
    64981 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
    65041 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
    65101 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
    65161 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
    65221 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
    65281 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
    65341 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
    65401 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
    65461 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
    65521 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
    65581 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
    65641 tgctataact aatcacagcg ccgaagggga tgggtttacc ctctttctgt tttaaggtta
    65701 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
    65761 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
    65821 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
    65881 tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
    65941 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
    66001 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
    66061 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
    66121 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
    66181 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
    66241 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttccgat
    66301 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
    66361 tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg
    66421 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
    66481 agg
//
LOCUS       DAJTYH010000008        65500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTYH010000008 DAJTYH010000000
VERSION     DAJTYH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..258)
                     /gene="folK"
                     /locus_tag="OO609_000517"
     CDS             complement(<1..258)
                     /gene="folK"
                     /locus_tag="OO609_000517"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6632054.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVFSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRVELQQGRVRK"
     gene            complement(255..1673)
                     /gene="pcnB"
                     /locus_tag="OO609_000518"
     CDS             complement(255..1673)
                     /gene="pcnB"
                     /locus_tag="OO609_000518"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6632055.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1712..2638)
                     /gene="gluQRS"
                     /locus_tag="OO609_000519"
     CDS             complement(1712..2638)
                     /gene="gluQRS"
                     /locus_tag="OO609_000519"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6632056.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2675..3130)
                     /gene="dksA"
                     /locus_tag="OO609_000520"
     CDS             complement(2675..3130)
                     /gene="dksA"
                     /locus_tag="OO609_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6632057.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3308..4012)
                     /gene="sfsA"
                     /locus_tag="OO609_000521"
     CDS             complement(3308..4012)
                     /gene="sfsA"
                     /locus_tag="OO609_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6632058.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4027..4557)
                     /gene="thpR"
                     /locus_tag="OO609_000522"
     CDS             complement(4027..4557)
                     /gene="thpR"
                     /locus_tag="OO609_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6632059.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4631..7060
                     /gene="hrpB"
                     /locus_tag="OO609_000523"
     CDS             4631..7060
                     /gene="hrpB"
                     /locus_tag="OO609_000523"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6632060.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7256..9790
                     /gene="mrcB"
                     /locus_tag="OO609_000524"
     CDS             7256..9790
                     /gene="mrcB"
                     /locus_tag="OO609_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6632061.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10010..12253
                     /gene="fhuA"
                     /locus_tag="OO609_000525"
     CDS             10010..12253
                     /gene="fhuA"
                     /locus_tag="OO609_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6632062.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12304..13101
                     /gene="fhuC"
                     /locus_tag="OO609_000526"
     CDS             12304..13101
                     /gene="fhuC"
                     /locus_tag="OO609_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6632063.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13101..13991
                     /gene="fhuD"
                     /locus_tag="OO609_000527"
     CDS             13101..13991
                     /gene="fhuD"
                     /locus_tag="OO609_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6632064.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            13988..15970
                     /gene="fhuB"
                     /locus_tag="OO609_000528"
     CDS             13988..15970
                     /gene="fhuB"
                     /locus_tag="OO609_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6632065.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16005..17285)
                     /gene="hemL"
                     /locus_tag="OO609_000529"
     CDS             complement(16005..17285)
                     /gene="hemL"
                     /locus_tag="OO609_000529"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6632066.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17510..18931
                     /gene="clcA"
                     /locus_tag="OO609_000530"
     CDS             17510..18931
                     /gene="clcA"
                     /locus_tag="OO609_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6632067.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18955..19020
                     /locus_tag="OO609_000531"
     CDS             18955..19020
                     /locus_tag="OO609_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632068.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19013..19357
                     /gene="erpA"
                     /locus_tag="OO609_000532"
     CDS             19013..19357
                     /gene="erpA"
                     /locus_tag="OO609_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6632069.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19404..20027)
                     /locus_tag="OO609_000533"
     CDS             complement(19404..20027)
                     /locus_tag="OO609_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6632070.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20065..20865)
                     /gene="btuF"
                     /locus_tag="OO609_000534"
     CDS             complement(20065..20865)
                     /gene="btuF"
                     /locus_tag="OO609_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6632071.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20858..21556)
                     /gene="mtnN"
                     /locus_tag="OO609_000535"
     CDS             complement(20858..21556)
                     /gene="mtnN"
                     /locus_tag="OO609_000535"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6632072.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21640..23157
                     /gene="dgt"
                     /locus_tag="OO609_000536"
     CDS             21640..23157
                     /gene="dgt"
                     /locus_tag="OO609_000536"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6632073.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23287..24711
                     /gene="degP"
                     /locus_tag="OO609_000537"
     CDS             23287..24711
                     /gene="degP"
                     /locus_tag="OO609_000537"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6632074.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24866..26022
                     /gene="cdaR"
                     /locus_tag="OO609_000538"
                     /pseudo
     CDS             24866..26022
                     /gene="cdaR"
                     /locus_tag="OO609_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26111..26497)
                     /locus_tag="OO609_000539"
     CDS             complement(26111..26497)
                     /locus_tag="OO609_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6632075.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26811..27635)
                     /gene="dapD"
                     /locus_tag="OO609_000540"
     CDS             complement(26811..27635)
                     /gene="dapD"
                     /locus_tag="OO609_000540"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6632076.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27666..30338)
                     /gene="glnD"
                     /locus_tag="OO609_000541"
     CDS             complement(27666..30338)
                     /gene="glnD"
                     /locus_tag="OO609_000541"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6632077.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30400..31194)
                     /gene="map"
                     /locus_tag="OO609_000542"
     CDS             complement(30400..31194)
                     /gene="map"
                     /locus_tag="OO609_000542"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6632078.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31562..32287
                     /gene="rpsB"
                     /locus_tag="OO609_000543"
     CDS             31562..32287
                     /gene="rpsB"
                     /locus_tag="OO609_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6632079.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32545..33396
                     /gene="tsf"
                     /locus_tag="OO609_000544"
     CDS             32545..33396
                     /gene="tsf"
                     /locus_tag="OO609_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6632080.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33543..34268
                     /gene="pyrH"
                     /locus_tag="OO609_000545"
     CDS             33543..34268
                     /gene="pyrH"
                     /locus_tag="OO609_000545"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6632081.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34560..35117
                     /gene="frr"
                     /locus_tag="OO609_000546"
     CDS             34560..35117
                     /gene="frr"
                     /locus_tag="OO609_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6632082.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35209..36405
                     /gene="ispC"
                     /locus_tag="OO609_000547"
     CDS             35209..36405
                     /gene="ispC"
                     /locus_tag="OO609_000547"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6632083.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36594..37352
                     /gene="ispU"
                     /locus_tag="OO609_000548"
                     /gene_synonym="uppS"
     CDS             36594..37352
                     /gene="ispU"
                     /locus_tag="OO609_000548"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6632084.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37365..38222
                     /gene="cdsA"
                     /locus_tag="OO609_000549"
     CDS             37365..38222
                     /gene="cdsA"
                     /locus_tag="OO609_000549"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6632085.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38234..39586
                     /gene="rseP"
                     /locus_tag="OO609_000550"
     CDS             38234..39586
                     /gene="rseP"
                     /locus_tag="OO609_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6632086.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39616..42048
                     /gene="bamA"
                     /locus_tag="OO609_000551"
     CDS             39616..42048
                     /gene="bamA"
                     /locus_tag="OO609_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6632087.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42170..42655
                     /gene="skp"
                     /locus_tag="OO609_000552"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42170..42655
                     /gene="skp"
                     /locus_tag="OO609_000552"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6632088.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42659..43684
                     /gene="lpxD"
                     /locus_tag="OO609_000553"
     CDS             42659..43684
                     /gene="lpxD"
                     /locus_tag="OO609_000553"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6632089.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43789..44244
                     /gene="fabZ"
                     /locus_tag="OO609_000554"
     CDS             43789..44244
                     /gene="fabZ"
                     /locus_tag="OO609_000554"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6632090.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44248..45036
                     /gene="lpxA"
                     /locus_tag="OO609_000555"
     CDS             44248..45036
                     /gene="lpxA"
                     /locus_tag="OO609_000555"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6632091.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45036..46184
                     /gene="lpxB"
                     /locus_tag="OO609_000556"
     CDS             45036..46184
                     /gene="lpxB"
                     /locus_tag="OO609_000556"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6632092.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46181..46777
                     /gene="rnhB"
                     /locus_tag="OO609_000557"
     CDS             46181..46777
                     /gene="rnhB"
                     /locus_tag="OO609_000557"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6632093.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46814..50296
                     /gene="dnaE"
                     /locus_tag="OO609_000558"
     CDS             46814..50296
                     /gene="dnaE"
                     /locus_tag="OO609_000558"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6632094.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50309..51268
                     /gene="accA"
                     /locus_tag="OO609_000559"
     CDS             50309..51268
                     /gene="accA"
                     /locus_tag="OO609_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6632095.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51367..53508
                     /gene="ldcC"
                     /locus_tag="OO609_000560"
     CDS             51367..53508
                     /gene="ldcC"
                     /locus_tag="OO609_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6632096.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53565..53954
                     /locus_tag="OO609_000561"
     CDS             53565..53954
                     /locus_tag="OO609_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6632097.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54019..55317
                     /gene="tilS"
                     /locus_tag="OO609_000562"
     CDS             54019..55317
                     /gene="tilS"
                     /locus_tag="OO609_000562"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6632098.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55366..55626)
                     /gene="rof"
                     /locus_tag="OO609_000563"
     CDS             complement(55366..55626)
                     /gene="rof"
                     /locus_tag="OO609_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6632099.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55613..55813)
                     /locus_tag="OO609_000564"
     CDS             complement(55613..55813)
                     /locus_tag="OO609_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6632100.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55979..56524
                     /locus_tag="OO609_000565"
     CDS             55979..56524
                     /locus_tag="OO609_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6632101.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56521..56943
                     /gene="arfB"
                     /locus_tag="OO609_000566"
     CDS             56521..56943
                     /gene="arfB"
                     /locus_tag="OO609_000566"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6632102.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56957..57667
                     /gene="nlpE"
                     /locus_tag="OO609_000567"
                     /gene_synonym="cutF"
     CDS             56957..57667
                     /gene="nlpE"
                     /locus_tag="OO609_000567"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6632103.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(57822..58646)
                     /locus_tag="OO609_000568"
     CDS             complement(57822..58646)
                     /locus_tag="OO609_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6632104.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58699..60417)
                     /gene="proS"
                     /locus_tag="OO609_000569"
     CDS             complement(58699..60417)
                     /gene="proS"
                     /locus_tag="OO609_000569"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6632105.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60528..61235)
                     /gene="tsaA"
                     /locus_tag="OO609_000570"
     CDS             complement(60528..61235)
                     /gene="tsaA"
                     /locus_tag="OO609_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6632106.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61232..61636)
                     /gene="rcsF"
                     /locus_tag="OO609_000571"
     CDS             complement(61232..61636)
                     /gene="rcsF"
                     /locus_tag="OO609_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6632107.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61754..62569)
                     /gene="metQ"
                     /locus_tag="OO609_000572"
     CDS             complement(61754..62569)
                     /gene="metQ"
                     /locus_tag="OO609_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6632108.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62609..63262)
                     /gene="metI"
                     /locus_tag="OO609_000573"
     CDS             complement(62609..63262)
                     /gene="metI"
                     /locus_tag="OO609_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6632109.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63255..64286)
                     /gene="metN"
                     /locus_tag="OO609_000574"
     CDS             complement(63255..64286)
                     /gene="metN"
                     /locus_tag="OO609_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6632110.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64474..65049
                     /gene="gmhB"
                     /locus_tag="OO609_000575"
     CDS             64474..65049
                     /gene="gmhB"
                     /locus_tag="OO609_000575"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6632111.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65413..>65500
                     /locus_tag="OO609_000576"
     rRNA            65413..>65500
                     /locus_tag="OO609_000576"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15479 a        16239 c        18067 g        15715 t
ORIGIN      
        1 tttacgaaca cgaccctgtt gcagttctac gcgctgtgta tggttgagta actcttccgg
       61 ggcgggcgaa gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc
      121 cagcggtggg gtgcggtaaa acgaaaaaac ggcaagaatg cggctttcag ggatatcgcc
      181 taatgctttc agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc
      241 aatatacgcc actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga
      301 gtacgacgac gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt
      361 ggtgcggaaa cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt
      421 tcaacttcag ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc
      481 agtttccatg cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg
      541 cgggttaacg tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg
      601 tcgttcatcg ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc
      661 tgtgccgtct ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg
      721 cgcatatcgt tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc
      781 atcgggctgt cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac
      841 agatgatatt cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta
      901 agcgattctt caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg
      961 atcggttctg cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg
     1021 cgcagcatac gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata
     1081 acgccgtcct tcagatcctt cataccacca acgtaatcac ggacggtaaa atccgctacg
     1141 ctgtaataca ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg
     1201 aaaatgttgt cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg
     1261 acgttacctt cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt
     1321 acatgagcca gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc
     1381 tgctcaggcg tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg
     1441 tcgcgcacgc cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt
     1501 accttcaggg cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc
     1561 accgtcacct gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc
     1621 ttgcggcaaa aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat
     1681 tcaggacaaa aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat
     1741 ttacaattgc cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga
     1801 tttgctcgac gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg
     1861 ccgcgattag taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg
     1921 aaagtttagc gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc
     1981 agccaaaaag ctggtacagc gggatttgcc ttactgttgg ttcaatcaga tcagccccac
     2041 gcactatttc tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga
     2101 acaacccatc acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa
     2161 taatgcctcg cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg
     2221 cggcgttgtc tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc
     2281 tttgaatacg cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg
     2341 cgagtgcttc acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt
     2401 gcagaccgta atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag
     2461 gcgggtcgat atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat
     2521 agctgccgag cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga
     2581 agcggccaat atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt
     2641 cccgcctgtg gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa
     2701 tttcagccag cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc
     2761 gaataccaat ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt
     2821 tcagcgtctt ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga
     2881 ggctgaactc ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat
     2941 cctgcatatg tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca
     3001 gaatacgacg gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct
     3061 gatatggttc caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc
     3121 cttcttgcat gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat
     3181 gaggccgcta taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga
     3241 caactgtgtg aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact
     3301 tactttacta caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt
     3361 ccgctttgta agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt
     3421 atttctcatc gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga
     3481 aaaaaataac cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct
     3541 gacctcgttc agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg
     3601 atttcacttc aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc
     3661 tgcgttcgga gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa
     3721 ttgattcatt aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa
     3781 taaatgcgcc gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt
     3841 tgtctgaagt cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg
     3901 tattcgggca gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac
     3961 gtttgtaacg ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa
     4021 tccttgttat tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc
     4081 aaacgaggag gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg
     4141 cgggaatgtc accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg
     4201 attgctttga aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat
     4261 taagccgcgt ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc
     4321 ggcgtcatca agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag
     4381 cgccttctct ttctctgcgc tcacttcgcc taaaaatgcc agagtcaggt gcaaattatc
     4441 ggcggcgacc ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg
     4501 ttcgcggatt tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg
     4561 aggcactcgg ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct
     4621 ggagcgtttt gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct
     4681 cgattgtgcg ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct
     4741 gccgctgcaa ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg
     4801 tcgtctggcg gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg
     4861 cgataccgtt ggctaccgga tgcgtgcgca aaactgcgtc gggctgaata cccgcctgga
     4921 agtggttacc gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt
     4981 tggattggtg atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc
     5041 gctgttactc gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc
     5101 tacgctggac aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga
     5161 agggcgctcg tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga
     5221 cgaagccgtt gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt
     5281 atttttacct ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg
     5341 cagtgatgtg gtgctctgcc cgctgtatgg cgtgttgtca ctgaacgatc agcgaaaagc
     5401 gatcctcccg gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac
     5461 cagtttaacc atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg
     5521 ctttgatccg cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat
     5581 gacgcagcgt gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc
     5641 caaagaacaa gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct
     5701 ttccggtttg ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg
     5761 gctggatcaa ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg
     5821 ggcgctggag ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga
     5881 tccgcgttta gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc
     5941 aaaaattgcc gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc
     6001 gttttcgcgc aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa
     6061 cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc
     6121 cgatcgcatt gctcatcgcc gtgggcaaga tggacgctat caactggcaa acagcatggg
     6181 agcgatgctc gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt
     6241 attgcagggc agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga
     6301 tgaattagta caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga
     6361 cgcgcaaggt acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt
     6421 gcagccgctg gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg
     6481 tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt
     6541 gttatgcgcc gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt
     6601 gttggcgacg ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg
     6661 cctgaaatca ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca
     6721 acgtctggat agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat
     6781 tcgttatcat gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga
     6841 ggccactaat ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc
     6901 tgcccaaagg ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg
     6961 tgaggtgcaa aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc
     7021 aaacacggca ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc
     7081 ctgtgggaga gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc
     7141 tccctcaagg gagaggggac cgatcgagcg caaattttga gagatatctt cttctgtctt
     7201 gtaacagaag aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc
     7261 cgggaatgac cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa
     7321 ggtaagccgt cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga
     7381 tgaagaaccg atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa
     7441 acgcggctgg ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat
     7501 ttacggcgtt tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact
     7561 gcccgcggca gtttatggcc gaatggtcaa ccttgaacca gatatgacca tcagcaagaa
     7621 cgaaatggtg aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa tgacccgtcc
     7681 tggcgaattt accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc
     7741 ggacagtaaa gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc atctggcgac
     7801 catcgtcaat atggagaaca accgtcagtt cggtttcttc cgtcttgatc cgcgtctgat
     7861 caccatgatc tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca gtggtttccc
     7921 tgatttgctg gtggatactt tgctggcgac agaagaccgt catttttacg agcatgatgg
     7981 aatcagtctc tactccattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt
     8041 gcagggtgcg agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg
     8101 ttcttactgg cgtaaagcga acgaagctta catggcgctg atcatggacg cgcgttacag
     8161 caaagaccgt attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa
     8221 cgaaatccgc ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct
     8281 aagcctcgac cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc
     8341 gtggcgtaac ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca
     8401 gcaacagatt attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca
     8461 gccgcgtggt ggggtgatct ctcctcagcc agcctttatg cagctggtgc gtcaggagtt
     8521 gcaggcaaaa ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt
     8581 tgactcggtg gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa
     8641 gaaacagcgt aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg
     8701 tgaagttcgt gcgatggtcg gaggttctga gccgcagttt gcgggctaca accgtgcgat
     8761 gcaggcgcgt cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag
     8821 ccagccgaaa atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca
     8881 gccgaatggc caggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag
     8941 agtgatgctg gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa atctggggat
     9001 ggcgctgggg ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac cgaaagatca
     9061 gttgcatccg gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc
     9121 gcaggcattc cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc
     9181 ggtaatcgcg gaagatggta aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt
     9241 tccggcgcag gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac
     9301 gggtcgtcag cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac
     9361 caacaataac gtagatacct ggtttgcagg cattgacggc agcacggtga ccatcacctg
     9421 ggtaggccgt gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat
     9481 ttatcagcgt tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga
     9541 tattgcagat atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt
     9601 cttgccggtc tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca
     9661 gccgtcaggc aatccgtttg atcagtcttc tcagccgcag caacagccgc aacagcaacc
     9721 tgctcagcaa gagcagaaag acagcgacgg tgtagccggt tggatcaagg atatgtttgg
     9781 tagtaattaa catctaagcg tgaaataccg gatggcgagt tgccatccgg taaaataaca
     9841 tcccatctaa gatattaacc ctttcttttc atctggttgt ttattaaccc ttcaggaacg
     9901 ctcagattgc gtaccgcttg cgaacccgcc agcgtttcga atattatctt atctttataa
     9961 taatcattct cgtttacgtt attattcact ttacatcaga gatataccaa tggcgcgttc
    10021 caaaactgct cagccaaaac actcactgcg taaaatcgca gttgtagtag ccacagcggt
    10081 tagcggcatg tctgtttatg cacaggcagc ggttgaaccg aaagaagaca ctatcaccgt
    10141 taccgctgca cctgcgccgc aagaaagcgc atgggggcct gctgcaacta ttgcggcgcg
    10201 gcagtcagct accggcacta aaaccgatac gccgattcaa aaagtgccac agtctatttc
    10261 tgttgtgacc gccgaagaga tggcgctgca tcagccgaag tcggtaaaag aagcgcttag
    10321 ctacacgccg ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg accacctgat
    10381 cattcgcggt tttgcggcag aaggccaaag ccaaaataac tatctgaatg gcctgaagtt
    10441 gcagggcaac ttctataacg atgcggtcat tgacccgtat atgctggaac gcgctgaaat
    10501 tatgcgtggc ccggtttccg tgctttacgg taaaagcagt cctggcggtc tgttgaatat
    10561 ggtcagcaag cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag ccggtactga
    10621 cagcctgttc cagactggtt ttgactttag cgatgcgttg gatgatgacg gtgtttactc
    10681 ttatcgcctg accggtcttg cgcgttctgc caatgcccag cagaaagggt cagaagagca
    10741 gcgttatgct attgcaccgg cgttcacctg gcgtccggat gataaaacca atttcacctt
    10801 cctttcttac ttccagaacg agccggaaac cggttattac ggctggttgc cgaaagaggg
    10861 aaccgttgag ccgctgccga acggtaagcg tctgccgaca gactttaatg aaggggcgaa
    10921 gaacaacacc tattctcgta atgagaagat ggtcggctac agcttcgatc acgaatttaa
    10981 cgacaccttt actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct cgcaaaacag
    11041 cgtttatggt tacggcgtct gctccgatcc ggcgaatgct tacagcaaac agtgtgcggc
    11101 attagcgcca gcggataaag gccattatct ggcacgtaaa tacgtcgttg atgatgagaa
    11161 gctgcaaaac ttctccgttg atacccagtt gcagagcaag tttgccactg gcgatatcga
    11221 ccacaccctg ctgaccggtg tcgactttat gcgtatgcgt aatgacatca acgcctggtt
    11281 tggttacgac gactctgtgc cactgttcaa tctgtacaat ccggtgaatc ccgatttcga
    11341 cttcaatgcc aaagatccgg caaactccgg cccttaccgc attctgaata aacagaaaca
    11401 aacgggcgtt tatgttcagg atcaggcgca gtgggataaa gtgctggtca ccctaggcgg
    11461 tcgttatgac tgggcagatc aagaatctct taaccgcgtt gctgggacga ccgataaacg
    11521 tgatgacaaa cagtttacct ggcgtggtgg tgttaactac ctgtttgata atggtgtaac
    11581 accttacttc agctatagcg aatcgtttga accttcttcg caagttggga aggatggtaa
    11641 tattttcgca ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg taccggaaga
    11701 tcgtccgatt gtagttactg gtgccgtgta taatctcact aaaaccaaca acctgatggc
    11761 ggaccctgag ggttccttct tctcggttga aggtggcgag atccgcgcac gtggcgtaga
    11821 aatcgaagcg aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt atacttacac
    11881 cgatgcggaa tacaccaccg atactaccta taaaggcaat acgcctgcac aggtgccaaa
    11941 acacatggct tcgctgtggg ctgactatac cttctttgac ggtccgcttt caggtctgac
    12001 gctgggcacc ggtggtcgtt atactggctc cagctatggt gatccggcta actcctttaa
    12061 agtgggaagt tatacggtcg tggatgcgtt agtgcgttat gatctggcgc gagtcggcat
    12121 ggcgggctcc aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat acgtcgccag
    12181 ctgctttaac acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac
    12241 ctttcgtttc taatttctct tttggggcac ggatttccgt gcccatttca caagttggct
    12301 gttatgcagg aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt
    12361 cgtgtgcccg ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg
    12421 accggtctga ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt
    12481 catcagcctc cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc
    12541 agcaaagcgt ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg
    12601 atgaccgtgc gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc
    12661 tttggtgcgg cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg
    12721 ctggcgcatc ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc
    12781 atgctggtgg cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc agcgctggat
    12841 atcgcccacc aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg
    12901 acggtcattg ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc
    12961 gccctgcgca gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa
    13021 accctcgaaa tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca
    13081 cctgtgagtt ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa
    13141 cggcgatggc gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg
    13201 accccaatcg tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca
    13261 tcgtgcctta cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat
    13321 tgccggactc agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg
    13381 aaatgaaacc gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg
    13441 cacgtattgc gccgggtcgc ggatttaact tcagtgacgg caaacatccg ttggcgatgg
    13501 cgcgtaaatc gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc
    13561 atttagcgca atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg
    13621 cacgcccgtt actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa
    13681 acagcttgtt ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa
    13741 ccaacttctg gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg
    13801 atgtgctctg ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc
    13861 tgtggcaggc catgccgttt gtccgtacag ggcgctttca gcgcgtaccc gcagtctggt
    13921 tttatggtgc aacgctttcg gcaatgcact ttgtgcgtgt tctggataac gccatcggag
    13981 gtaaagcgtg agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc tgttagtgat
    14041 tgtcgctacg gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca gtcagtgggc
    14101 gcaggccgcc tggtcgccgg atattgacgt catcgagcag atgatttttc actacagctt
    14161 gttgccgcgt ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct
    14221 gtttcagcaa gtactgcgta acccgctggc ggaaccgacg acgttgggcg ttgcaacagg
    14281 cgcgcaactg gggattaccg tcaccacgct ctgggcgatc cctagcgcga tggcgagcca
    14341 gtttgctgcg ctggcagggg cttgcgttgt tggcttaatt gtctttggcg tcgcgtgggg
    14401 gaaacggctg tcgccggtaa cgctgatttt cgcggggttg gtagtgagcc tttattgcgg
    14461 cgcaatcaat cagttactgg ttatcttcca tcatgaccag ttgcaaagca tgtttctgtg
    14521 gagcactgga acgctgacgc aaaccgactg gggcggtgtt gagcgtttat ggccgcagtt
    14581 gctgggcggt gtgatgctga cgttgctgct acttcgtccg ttaaccctga tggggcttga
    14641 tgatggcgtg gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg cggcgctgtc
    14701 gctggcgatt gtcatcagtg cgctgctggt gaacgctgtg gggattatcg gctttatcgg
    14761 gttgttcgcg ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc cacgactgat
    14821 gctggcgtcg ttgattggtg cgctgatcct ctggctttcc gatcaaatca tcctctggct
    14881 gactcgcgtg tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct
    14941 gctactgtgg ctgctgccgc gtttacgcag cattagcgcg ccggatatga aggttaacga
    15001 tcgtgtcgcg actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgtt
    15061 gttgatggct gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc
    15121 gagcggggcg ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta tggcggcgct
    15181 gtttgcgggc gtcatgctgg cggtagcggg ctgtattatt cagcgactga ccggcaaccc
    15241 gatggcaagc ccggaagtgc tggggattag ctccggcgcg gcgtttggtg tggtgttaat
    15301 gctgtttctg gtaccgggta atgcctttgg ctggttgtta cccgcaggca gtctcggggc
    15361 ggcggtgacg ctgttgatca ttatgatcgc cgccggacgc ggtggatttt ccccacaccg
    15421 catgttactg gcggggatgg cgttaagtac cgcgttcacc atgcttttga tgatgttgca
    15481 ggcaagtggt gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa
    15541 cgcgaccgat gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc tggcgattac
    15601 tccgctgtgc cgccgctggc tgaccatttt accgctgggt ggtgataccg cgcgggcggt
    15661 agggatggcg ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc
    15721 gaccgcgacg atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc
    15781 gcggatgatg ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg
    15841 tttactgctg gtgttcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc
    15901 ggcggggctg ctgtcgacct ttatcggtgc gccatatttt atctatttgc tgagaaagca
    15961 gagccgttaa tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa cttcgcgaac
    16021 acccgacgtg cagcatcgat ggtgttattg atatcttcca tgctgtgcgc cacagacata
    16081 aagcctgctt caaacgctga cggtgccagg taaacacctt catccagcat catatggaag
    16141 aaacgcttaa aacgctcgac gtcacaggcc atcacatcct gatagcacgt cacggactcg
    16201 gcgtcggtaa agaagatacc gaacatacca ccgacgtggt taacgaccag cggaattccc
    16261 gcttcttctg ccgcttccag cagaccttct gccagacgtg ttgtcagctc atccagcgtt
    16321 tcgtgaatgc ccggctgcgc gacttcattc agacaggcga aacccgctgc catcgcaatc
    16381 gggttaccgg aaagcgtacc cgcctgatag accggacctg tcggggccag cgcatccatt
    16441 acatcacgac gaccaccgaa tgcgcctacc ggcattccac cgccgatgat ttttccgagg
    16501 caggttaaat ctggcactac ggcgaaataa tcctgtgcgc cagctagcgc tacgcggaaa
    16561 ccggtcatca cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag cgcacgcaga
    16621 cctggcagga actctggcag cggcggaaca cagttcatat tgcctgccac cggctcgacg
    16681 ataatacagg caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga ggccagatcg
    16741 ttataagtac aggttaaggt atgtttggcg aaatctgccg gaacgcccgg cgagtttggc
    16801 tggcctaacg tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc gtgaccgtgg
    16861 taacaacctt caaatttaat gattttgtcg cgaccggtaa aaccacgggc caggcggatg
    16921 gcgctcatcg tcgcctcggt gccggagttc accatgcgca ccatatccat ggtcgggacc
    16981 agttcggtca ccagttgcgc cattttcact tccatttcgg tcggtgcacc aaagcttaaa
    17041 ccacgctcgg cggcttcaat cacggcattg cggatcgccg gatggttatg gcccagcacc
    17101 atcggccccc aggaaccgac ataatcgata taggctttgc catcaacatc gtacagataa
    17161 gcgccgtccg ctttttcgat aaacagtgga gtgccgccca cgccagtaaa ggcgcgaaca
    17221 ggggagttca caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt ttcagactta
    17281 ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct gctattccat gttattcata
    17341 acaagttaaa tactcgtcaa acatcaggct gcttgtactg gtgcaatcct gatttcttag
    17401 agtataaaag ttttgtgcat ttgaaacatt acgctttgca aaggattttc atggaacgtg
    17461 cgagtaaaat gccgtcatct tatttgtatg accaataagt gatcattgga tgaaaactga
    17521 tactccctct ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac tgattcgcca
    17581 acttcttgag cgcgataaaa ccccgttagc cattttgttt atggcggcag tcgtcggcac
    17641 gcttgtcggg ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc agaaccaacg
    17701 tatgggggcg ctggtacata ctgctgataa ttatccgcta ctgttaaccg tcgcttttct
    17761 ctgttcggcg gtgctggcga tgttcggcta ctttttggtg cgcaaatacg cgcccgaagc
    17821 agggggatcg gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc ctgttcgctg
    17881 gtggcgtgta ttgccggtga aattctttgg cgggctgggg acactcggcg gaggcatggg
    17941 gctagggcgc gaaggaccaa ccgtgcagat cggcggcaat atcagtcgca tggtgcttga
    18001 tattttccgc ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg gtgctgctgc
    18061 ggggctggct gcggccttta acgcgccgct ggcgggtatt ttgtttatta tcgaagagat
    18121 gcgtccgcag tttcgctata cgttaatttc gattaaagcg gtatttattg gcgtcattat
    18181 gtcgaccatt atgtaccgga tttttaatca tgaagttgcg ttgattgacg tcggtaaact
    18241 ttctgatgcg ccgcttaata cgctgtggct ttatctgatc ctcggtatta tttttggcat
    18301 tttcggccct atttttaata aatgggtgct ggggatgcag gatttgctgc accgtgtgca
    18361 cggcggcaat attaccaaat gggtactgat ggggggggcg attggcggtc tgtgtggatt
    18421 gctggggttt gtggcaccag caacgtcggg cggcggtttt aacctgattc ctatcgctac
    18481 cgcggggaat ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg tcattaccac
    18541 cttactctgc ttctcttccg gcgcgccggg cggtattttt gccccgatgc tggcgctggg
    18601 tactgtgctg ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc aatatcacct
    18661 ggaggcgggg acgtttgcta ttgccggaat gggggcatta ctggcggcat ctattcgcgc
    18721 gccgttaacg gggatcattc tggttctgga gatgaccgat aactaccagc tcattttgcc
    18781 aatgattatt accggtcttg gcgcaacact attagcgcaa tttaccggcg ggaaacctct
    18841 atactcggcg attcttgcgc gcacgctggc aaaacaggaa gctgagcaac tggcgcgaag
    18901 caaggccgca tcagccagcg agaatacttg aacgaaatac cagggtatta gataatggcg
    18961 attattattg ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa atatgagtga
    19021 tgacgtagca ctgccgctgg agtttaccga cgcagcagcc aacaaagtta aaagcctgat
    19081 cgctgacgaa gataacccga atctgaaatt acgcgtgtat atcaccggtg gcggttgcag
    19141 cggcttccag tatggtttca cctttgatga tcaggtgaac gaaggcgata tgaccatcga
    19201 aaaacagggc gttggcctgg tggttgatcc gatgagcctg caatatctgg tcggcggttc
    19261 cgttgactac accgaaggac tggaaggttc tcgtttcatc gtgaccaacc cgaacgcgaa
    19321 aagcacctgc ggttgcggtt cttcctttag catctaatct gttgttcatg tgccggataa
    19381 actatccggc acatcttctc tcttcaacgc ccattctcat ccagcgcaaa cgtcggtagc
    19441 ttaagatgcc aacgaatagc cgccagccga atcaatagcg tcacgaccat gcccatcata
    19501 ctggctgttt ccagtggtac ggaaaatgtg taataagccg tagcgtggac aataccgccg
    19561 ataatacagg cagttgcgta gatttctgta cgtaaaatca tggggatttc gcggaccagt
    19621 acatcacgaa tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac cgcgattaac
    19681 ggaccggctt ccgcattaaa ggctttattc acgctaatgc cgacaaacac cgccagacca
    19741 acggcgtcca gcaccggcaa catccatttt ggtaagcgtc ttggctggcg caccagcacg
    19801 atggtcagca tgctggtgac catggcaacg accagatcgg tgggatcttt cacccaaaat
    19861 accgggccgt gatccagcgc catgtcgcga attgttccgt cgcctactgc ggtaaccacg
    19921 cccagtacca gaacaccaaa agggtccata cgcaattttc cggctaacaa aacgccgtag
    19981 atggcaaata ccgctgtgcc gactatatcc agccaataga cgagcattgt tcaatcccca
    20041 ctgagcacct gaaaaggtca ggcactaatc tacctgtgaa agcgcattac agagctgttg
    20101 tgctgcgagg ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga gaggaataac
    20161 gggaattttg agctgttcac cccagtattg tttgatttta ggaatttggt ccggtccgcc
    20221 tgtaatgaca atagcctgtg gcgagcgtgc taacacctgt tcgcggctaa cttgcggcca
    20281 gggaacccgg ctgtctttaa agatgttttc tccgccacaa acttcgagta cctggttctg
    20341 aatcgactct tttccactgg taaatggcgg attaatgccg aattgcagaa aaacacgttt
    20401 tttaggttta tcagcatatt gcgctttcaa ttgcacgtac tgatccagca gggattgcgc
    20461 ggcttgttcg gccttgtctg gttgcggact ccagggggcc agttgacgta acgcattggc
    20521 aatttgttca atgcttgtcg catcgaccca catcactttt attcccagcg aagccagctg
    20581 gtcaacctgc cgctcggcat tacctccacg ccaggcaatc accagatcgg gtttcagcgc
    20641 gacaatgcgt tccagattca tcccctgcca ggtggaaacc tgctcaatct tttgcgcttg
    20701 tggaggatag tcggaatagc tgctgacccc aaccggcgtg atcccggcgg caaaggcaag
    20761 ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc acagtggcgc
    20821 gagaaaagac agggcgacca gcgccctgaa cagtgattta gccatgtgcc agtttctgca
    20881 ccagtgactc aaccatcagg ctggactgtt tagcggcaac agccaggaac tcatcgaagc
    20941 taagatgaga ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca aacgggacgt
    21001 tgaaattgtg gcagacatgg gcgattgccg tcgcttccat ctctacagca atggcctgtg
    21061 ggaagttgtg gcggattttc gccagaccaa cagaaccgtt gatgaaagcg tcgccgctaa
    21121 caatcaggcc acgtacagcg ttaagattca gttcggcaat gcaggcctca gcggcagcga
    21181 tcagtttatc gtcagcttta aagcctgccg gacagcctgg taactgaccg tattcataac
    21241 caaatgccgt gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg atatcgccca
    21301 ctttcagcgt tggtgccagg ccaccggcag aaccggtgtt aataatcaca tctggcttgc
    21361 agtgttccaa cagcagagtg gcacccagcg ccgcagcgac tttaccgatg cccgatttca
    21421 gaagcgcaac ctcggttcca ttcagttggc cggtatagat ttcgcaaccg ccgagactga
    21481 tagtttgacg gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc attgcaccaa
    21541 tgatgccgat tttcatagat ttactcgcga taagcccgat ttgaaggcat agtttaccat
    21601 gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca tggcacagat tgatttccga
    21661 aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac cgaacatgag
    21721 atcctgcgga tcttcgagag cgatcgcggg cgtatcatca actctccggc aattcgtcgt
    21781 ctgcaacaaa agacccaggt ttttccactg gagcgcaatg ccgccgtgcg cacgcgtctt
    21841 acccactcga tggaagtcca gcaggtggga cgctacatcg ccaaagaaat tttaagccgt
    21901 ctgaaagagc ttaaattgct ggaagcatac ggcctggatg aactgaccgg cccctttgaa
    21961 agcattgttg agatgtcatg cctgatgcac gatatcggca atccgccgtt tggtcatttt
    22021 ggcgaagcag cgataaatga ctggtttcgc caacgtttgc acccggaaga tgccgaaagc
    22081 cagcctctga ctgacgatcg ctgcagcgtg gcggcactac gtttacggga cggggaagaa
    22141 ccgcttaacg agctgcggcg caagattcgt caggacttat gtcattttga ggggaatgca
    22201 caaggcattc gtctggtgca tacattgatg cggatgaatc tcacttgggc acaggttggc
    22261 ggtattttaa aatatacccg tccggcgtgg tggcgtggcg aaacgcctga gacacatcac
    22321 tatttaatga aaaagccggg ttattatctt tctgaagaag cctatattgc ccgcttgcgt
    22381 aaagaactta atttggcgct ttacagtcgt tttccattaa cgtggattat ggaagctgcc
    22441 gacgacatct cctattgtgt ggcagacctt gaagatgcgg tagagaaaag aatatttacc
    22501 gttgagcagc tttatcatca tttgcacgaa gcgtggggcc agcatgagaa aggttcgctc
    22561 ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct caaatagttt aagccgcagt
    22621 acggaagatc agttttttat gtatttacgg gtaaacaccc taaataaact ggtaccctac
    22681 gcggcacaac gatttattga taatctgcct gcgattttcg ccggaacgtt taatcatgca
    22741 ttattggaag atgccagcga atgcagcgat cttcttaagc tatataaaaa tgtcgctgta
    22801 aaacatgtgt ttagccatcc ggatgtcgag cagcttgaat tgcagggcta tcgggtcatt
    22861 agcggattat tagagattta tcgtccttta ttaagcctgt cgttatcaga ctttactgaa
    22921 ctggtagaaa aagaacgggt gaaacgtttc cctattgagt cgcgcttatt ccacaaactc
    22981 tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta aattaccgtc agattctcct
    23041 gagtttccgc tatgggaata ttattaccgt tgccgcctgc tgcaggatta tatcagcggt
    23101 atgaccgacc tctatgcgtg ggatgaatac cgacgtctga tggccgtaga acaataacca
    23161 gtcttttgta aagacgaaca ataaattttt accttttgca gaaactttag ttcggaactt
    23221 cgggctataa aacgaatctg aagaacacag caattttgcg ttatctgtta atcgagactg
    23281 aaatacatga aaaaaaacac attagcactg agtgcactgg ctctgagttt aggtttggcg
    23341 ttatctccgc tctctgcaac ggcggctgag acttcttcag caacgacagc ccagcagatg
    23401 ccaagccttg caccgatgct cgaaaaggtg atgccttcag tggtcagcat taacgtagaa
    23461 ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt cttcggtgat
    23521 gattctccgt tctgccagga aggttctccg ttccagagct ctccgttctg ccaggggggc
    23581 cagggcggta atggcggcgg ccagcaacag aaattcatgg cgctgggttc cggcgtcatc
    23641 attgatgccg ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa cgcgacggtg
    23701 attaaagtcc agctgagcga tggtcgtaag ttcgacgcga agatggttgg caaagatccg
    23761 cgctctgata tcgcgctgat ccaaatccag aacccgaaaa aactgaccgc aattaagatg
    23821 gcggattctg atgcactgcg cgtgggtgat tacaccgtag cgattggtaa cccgtttggt
    23881 ctgggcgaga cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg cctgaatgct
    23941 gaaaactacg aaaacttcat ccagaccgat gcagcgatca accgtggtaa ctccggtggt
    24001 gcgctggtta acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct cgcaccggac
    24061 ggcggcaaca tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa cctgacctcg
    24121 cagatggtgg aatacggcca ggtgaaacgc ggtgagctgg gtattatggg gactgagctg
    24181 aattccgaac tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt cgtaagccag
    24241 gttctgccta attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt gatcacctca
    24301 ctgaacggta agccgatcag cagctttgcc gcactgcgtg ctcaggtggg tactatgccg
    24361 gtgggcagca aactgaccct gggcttactg cgcgacggta agcaggttaa cgtgaacctg
    24421 gaactgcagc agagcagcca gaatcaggtt gattccagct ccatcttcaa cggcattgaa
    24481 ggcgctgaga tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa cgtgaagacg
    24541 ggcactccgg ctgcgcagat cggcctgaag aaaggtgatg tgattattgg tgcgaaccag
    24601 caggcagtga aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc gtctgtgctg
    24661 gcactgaaca ttcagcgcgg cgacagcacc atctacctgt taatgcagta atctccctca
    24721 accccttcct gaatacggga aggggttctc cttacaatct gtgaacttcg ccacaactcc
    24781 atacatcttc atcatccttt gggcatttgc acaatgccgt acgttacgta cttccttatg
    24841 ctaagccgtg cataacggag gacttatggc tggctggcat cttgatacca aaatggcgca
    24901 ggatatcgtg gcacgtacca tgcgcatcat cgataccaat atcaacgtaa tggatgcccg
    24961 cgggcgaatt atcggcagcg gcgatcgtga gcgtattggt gaattgcacg aaggtgcatt
    25021 gctggtactt tcacagggac gagtcgtcga tatcgatgac gcggtagcac gtcatctgca
    25081 cggtgtgcgg caggggatta atctaccgtt acggctggaa ggtgaaattg tcggcgtaat
    25141 tggcctgaca ggtgaaccag agaatctgcg taaatatggc gaactggtct gcatgacggc
    25201 tgaaatgatg ctggaacagt cgcggttgat gcacttgttg gcgcaggata gccgtttgcg
    25261 ggaagaactg gtgatgaacc tgattcaggc agaggagaat actcccgcac ttctgaatgg
    25321 gcgcaacggc tggggatcga tctcaatcaa ccgcgagtgg tggctattgt tgaggtcgac
    25381 agcggtcagc ttggcgtgga cagcgcaatg gcggagttac aacaactgca aaacgcgctg
    25441 actacgcccg agcgtaataa tctggtggcg attgtctcgc taaccgaaat ggtggtgttg
    25501 aaaccggcgt tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa gcgagttgaa
    25561 caactgatta cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc actgggcaac
    25621 tattttaccg gtcctggcag tattgcccga tcctatcgta cggcgaaaac gacgatggtg
    25681 gtgggtaaac agcggatgcc agaaagtcgc tgctattttt atcaggatct gatgttacct
    25741 gtgttactcg acagtttgcg tggcgactgg caggccaacg aactggcgcg accgctggcg
    25801 cggctgaaag cgatggacaa taacggcttg ctgcgacgaa cgctggcggc gtggtttcgc
    25861 cacaatgtgc aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa taccctggag
    25921 tatcggctta atcgtatatc ggaactgacc gggcttgatt tgggcaattt tgatgacagg
    25981 ttgctgctgt atgtggcgtt acaactggat gaagagcggt agattatgcg ttaaggttga
    26041 ggcggcgctg gctcattccc ctcagcccga aggggagagg ggaatgcccg agttaaccgt
    26101 caattcggga ttatttacgc gttagttttt ccagatcggc ttcaatctcg ctgatcttat
    26161 tggttacgac tgactccagg tgacgtaagt cgtcgaggat cttacgctta agatcaactt
    26221 cgctgcggtc acgctgacag atttgatcaa gctcatcaat gatataccgt agattcgggc
    26281 tgatttcctg gacttcttta taaccctgac ccacaccatc agcgacgacc gttttacgct
    26341 gacgcggata tttaaactta acgctcttgg cgaaaaactc gcctttgtct ttctggaaat
    26401 agattttcag aatatcgttg ttggcttcct gccggaggct gtaacgatca atttcttcag
    26461 gattggtaat acccagactt ttcagattgt cgtacatagc gttacctcaa aatgagtcag
    26521 taaattgtgc ttattttagc atttggcttg gcccgcccgg ctcgactgtg atcgcagaaa
    26581 gcctttttct tccagacttc tatcatggcg taaaaatcaa aaaattacct gctttattgt
    26641 ggcgatattc catgggatgg attagcacat ttatcttcgt tttgcgaatt acatgacagt
    26701 ccgaaacaag atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag gacgaattat
    26761 tctgagccag aaagcatacg ggcagcatca tgctgcccgt atatgtactc ttaatcgatg
    26821 gtacgcagca gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac tttcttaacg
    26881 ataaccgcac agtagaggct gtatttgcca tcttttgacg gcagattacc tgaaacaacc
    26941 acagaccccg ccggaacgcg accgtagtgg atttcgccgg tttcacggtc gtaaatacgg
    27001 gtgctctgac caatgtatac gcccatagag atgacggaac cttcttcgac aatcacccct
    27061 tcaaccactt cagagcgtgc gccaatgaag cagttatctt caataatggt cgggttagcc
    27121 tgcagcggct ccagcacgcc gccgatgccc acgccaccgg aaaggtggac gtttttacca
    27181 atctgcgcac aagaaccgac ggtcgcccag gtatcaacca tggtgccttc atcaacatat
    27241 gcgccgatgt taacgtaaga cggcatcagc acggtgttac gggcaataaa cgcaccctga
    27301 cgtaccgccg ctggtggcac aacgcggaag ccttctttct ggaaacgtgc ttcgtcgtag
    27361 tcggcgaatt tcatcggcac tttgtcgaag tagcggcttt ctgccccttc gatcacctga
    27421 ttatcattaa tacggaaaga gagcagcacc gcttttttca accactgatg cgtcacccac
    27481 tgaccgtcaa ttttttccgc tacacgcagt gcgccggaat ccagcagggc gatcacctga
    27541 tttaccgctt cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc acggcgttca
    27601 aaagcggttt caataatgtt ctgtaactgc tgcattgtta aactcttttc atatcagtaa
    27661 acacatcacc ctttatcgtt tggattgagg gcctctgtca accgctgatg cacttcctgc
    27721 tgcaactcgt tattaagcgc acgccggtca gcggtggcaa ttatgaataa atcttctact
    27781 cgctcgccaa tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc ggcaaaaatt
    27841 ttcccgactc gcgccagcag tccaggttgg tcgagggcga tcagttcgag gaacgatttg
    27901 cggtcggtat gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg gcgtaatttg
    27961 gcgggttggc gacggggctg cggtggctgc cagctacttt gcgtcagcac ctgctccaga
    28021 ccaaaccgaa taacctcatg gcgatctgcg gacagcgggc tgccatcggg ttccagcacg
    28081 ataaaggtat ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc gtgaacactt
    28141 aaattgcggc ggtctaattc ggcacagacg gcggcaaaca gataggggcg gtccgggctc
    28201 cagataaaaa tctcggtgcc tccacgcgta gcctgcgggc taagcaatac cagcggtttg
    28261 cttaaatcat gctgtaataa atggcgggca tgccaggcca gttgatttgg gctatggcgg
    28321 acaaaatagt tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc gtcgatgtta
    28381 tccatgcgca gtagtgccag tgcctggagt tggtgatggc gaacccgttc gcgcatatcc
    28441 ggcgtgtttt gcattccgcg tcgtagctgc ttttcggtgg caaaatagag ttcgcgcaac
    28501 agactttgct tccagctatt ccacagtgtt tcgttggtgg cgcaaatgtc agccactgtc
    28561 aggcatacca gatagcgcag acgattttcc gtttgaactt cttcggcaaa ctgcttgatg
    28621 acttcagggt cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa gtgctggcga
    28681 accagccagg caaccagctg tgtttcgcgt gagttcagcc cgtggagttc tgcaaaatgc
    28741 actacatcct gagcaccgag aatggagtgg tcgccgccgc gtcctttggc gatatcgtga
    28801 aacagcgcgg cgatgaaaat cagctcagtt gacggcaggc gcggccagac gtccacacac
    28861 aacggatggc gctggcgcgt ttcttcactg gcaaaactct ccagtttcag catcacgcgg
    28921 atagtatgtt catccaccgt gtaggcgtgg aacagatcaa actgcatctg cccgacgata
    28981 tgcgaccatt gcggcatata cgcgccgagc acgctatggc gatgcattgg caatagtccg
    29041 cgccgcaccg ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc ttgcggaata
    29101 ttacacagcg gttgttgcag atggcgacgg gcatggcgta actggcgcag cgtggtggag
    29161 taaatgccgg tgatcgcact gttgcgcacc atggtgtaga acatacgcaa gatggcttcc
    29221 ggctggcgca taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg tagctgaaac
    29281 tcatcgtcga gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc ttcatcgaac
    29341 agttgcagca gcatctggtt gagttcactg acgcggcgtg taacgcggaa gtaatccttc
    29401 atcatccgct cgaccggttc gttgccttca ccactgtaat tcagacgctg agcgacgcta
    29461 agttggcgat cgaataacag acgattatcg taacggctga cgaccagatg cagggcaaag
    29521 cgaatacgcc acagtatatg cagacattcg tttaattccg cccgctccgc tgaggttaag
    29581 aagccaaacc cgaccatttc atccagcgat gttgcgccaa aatgacggcg ggccacccat
    29641 tgcagagtgt gaatatcgcg caagccgcca gggctacttt tgatgtctgg ttcaaggttg
    29701 tagctggtgc catggtaacg ctgatggcgc tggttctgtt cttcaacttt cgctgcgtag
    29761 aacttgtcgg aaggccagaa tccttcgcta aaaatatgtt tttgcagttc gaggaagagc
    29821 gcaacatcgc caattaataa gcgggattcg attaaattgg tggcgacggt taaatccgat
    29881 aacccttcca acatgcactc ttcaagcgtg cgcacactat gaccgacttc cagctttaca
    29941 tcccagagca gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg gagcttttta
    30001 cggcttaaaa tcagtaaatc gatgtcagaa agtggatgca gctcgccacg accgtagcca
    30061 ccgacggcaa ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat ccataatcgt
    30121 tgcaggagct ggtctatgaa ctcggtacgt gcctcaatca actgttctgc agagatccca
    30181 ttgtcaaagg catcacccag ccaacgctgg aaagtatcga tatgggcttt tatcccacag
    30241 acggttaatt catcacgggg ccaggcgcat ggattttgcg gttgaccggg cagggtggag
    30301 agagcggtgt ttgcgtactg ttctggaagg gtattcattg tgcgccaccc ataagattaa
    30361 attatcgcat taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt gcgagattat
    30421 cgccgggatg gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat cagtcaccac
    30481 aatagtatgc tcatattgtg cagacaagct gcgatctttg gtttttaccg tccagccatc
    30541 tttcatggtg cggatctctt ttttacccgc gttgaccatt ggctcaatgg tgaacgtcat
    30601 ccccggtttc agcacgacgt tggtttcacg ggagtcatag tgcagcacct gcggttcttc
    30661 atggaagccg cgaccaatac cgtgtccgca atattcacga acgacggaga agccttctgc
    30721 ttcgacaaat ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg gttttaccat
    30781 gcgtagcgcc aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc ccatgatggt
    30841 cggcttaccg acgataaaca ttttcgaggt atcgccgtgg aaaccatctt tgattacggt
    30901 gacatcaatg ttaacgatat cgccatcttt cagcagctta gcatcgtccg ggataccgtg
    30961 gcacaccact tcattaatag agatgcaaac ggatttcgga tagccgtgat agccgaggca
    31021 ggcagaaacc gcgtgttgtt cattaacaat gtaatcatta cagatgcgat ccagctcgcc
    31081 ggtgctgacg cccggtttaa catacggttc gatcatctcc agcacttcgg cagccagtcg
    31141 gccagcgacg cgcatttttt cgatatcttc tggggtcttg attgagatag ccattaattc
    31201 tgtccatcag cgtcggtgat accgacaata tatatgtaag tgccgtcaat ggtatcacac
    31261 ccgggcaaat tgagaatcat tctgaatttc gccaaacgtg ccactgaagg ttttctataa
    31321 tagaaaattc gacgtctgat gctgtacaca gcgccaacaa ttattggtgt ccacgacgta
    31381 tttgtggtat aaagcgcgcc ggacttccga tccatttcgt atacacagac tggacggaag
    31441 cgacaatctc actttgtgta acaacacaca cgtatcggca catattccgg ggtgcccttt
    31501 ggggtcggta atatgggata cgtggaggca taaccccaac ttttatatag aggttttaat
    31561 catggcaact gtttccatgc gcgacatgct caaggctggt gttcacttcg gtcaccagac
    31621 ccgttactgg aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca aagttcacat
    31681 catcaacctt gagaaaactg taccgatgtt caacgaagct ctggctgaac tgaacaagat
    31741 tgtttctcgc aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa gcgaagcggt
    31801 gaaagacgct gctctgagct gcgaccagtt cttcgtgaac catcgctggc tgggcggtat
    31861 gctgactaac tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc tggaaactca
    31921 gtctcaggac ggtactttcg acaagctgac caagaaagaa gcgctgatgc gcactcgtga
    31981 gctggagaaa ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc tgccggacgc
    32041 tctgtttgta atcgatgctg accacgaaca cattgctatc aaagaagcaa acaacttggg
    32101 tattccggta tttgctatcg ttgataccaa ctctgatccg gacggtgttg acttcgttat
    32161 cccgggtaac gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg ttgctgcaac
    32221 cgtacgtgaa ggccgttctc aggatctggc ttcccaggcg gaagaaagct tcgtagaagc
    32281 tgagtaataa ggcttgataa ctcccccaaa atagttcgag ttgcagaaag gcggcaagct
    32341 cgagaattcc cgggagctta catcagtaag tgaccgggat gagcgagcga agacaacgca
    32401 tctgcggcgc gaaatatgaa gggggagagc ccttatagac caggtagtac acgtttggtt
    32461 agggggcctg catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca
    32521 gatcacatct ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg
    32581 cgtgagcgta ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc
    32641 gacatcgagc tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa
    32701 gcaggcaacg ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc
    32761 attctggaag ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc
    32821 gcagacaaag ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa
    32881 gcacagttcg aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt
    32941 cgccgcgttg ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc
    33001 ggcgttctgg ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac
    33061 gttgctgcaa gcaagccaga attcatcaaa ccagaagacg tatccgctga agtggtagaa
    33121 aaagaatacc aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca
    33181 gagaaaatgg ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag
    33241 ccgttcgtta tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa
    33301 gtgactggct tcatccgttt cgaagtgggt gaaggcatcg agaaagttga gactgacttt
    33361 gcagcagaag ttgctgcgat gtccaagcag tcttaattat caaaaaggag ccgcctgagg
    33421 gcggcttctt tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa
    33481 agcgacgtac aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa
    33541 acatggctac caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag
    33601 ctctgcaggg cactgaaggc ttcggtattg atgcaagcat actggatcgt atggctcagg
    33661 aaatcaaaga actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc
    33721 tgttccgtgg cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg
    33781 ggatgctggc gaccgtaatg aacggcttgg caatgcgtga tgcactgcac cgcgcctatg
    33841 tgaacgctcg tctgatgtcc gctattccat tgaatggcgt gtgcgacagc tacagctggg
    33901 cagaagctat cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta
    33961 acccgttctt taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt gaagccgatg
    34021 tggtgctgaa agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa
    34081 ccgcaaccat gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag ctgaaagtca
    34141 tggacctggc ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaata
    34201 tgaacaaacc gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca
    34261 cggaataatt cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggcg
    34321 taagtctggt tataaggcgt tattgttgca ggcagtttgg tcacggccag cgcgcagcaa
    34381 ccggagcgta caaaagtacg tgaggatggc gagcactgct cggggccaaa atggcaaata
    34441 aaatagccta ataatccaga cgattacccg taatatgttt aatcagggct atacttagca
    34501 cacttccact gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg
    34561 tgattagcga tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca
    34621 aaacccaaat cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg
    34681 tcgtggaata ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag
    34741 attcccgtac tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag
    34801 cgattatggc ctccgatctc ggcctgaacc cgaactctgc gggtagcgac atccgtgttc
    34861 cgctgccgcc gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag
    34921 cagaacaagc gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac aaagtgaaag
    34981 cactgttgaa agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac
    35041 agaaactgac tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag
    35101 aactgatgca gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat
    35161 cggctggcgg cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc
    35221 accattctgg gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat
    35281 cccgaacact tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa
    35341 cagtgcctgg aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt
    35401 cttaaaacga tgctacagca acaggggagc cgcaccgaag tcttaagtgg gcaacaagcc
    35461 gcttgcgata tggcagcgct tgaggaggtt gatcaggtga tggcagccat tgttggtgct
    35521 gctgggctgt tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat
    35581 aaagaatcac tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg
    35641 caattgttac cggtcgatag cgaacataac gctatttttc agagtttacc gcaacctatc
    35701 cagcataatc tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc
    35761 gggtctggtg gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat
    35821 caagcttgcc gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc
    35881 atgatgaaca aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc
    35941 cagatggaag tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac
    36001 ggcagtgttc tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg
    36061 gcatggccga atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg
    36121 ttgacatttg ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc gatggaggcg
    36181 ttcgaacaag gccaggcagc gacgacagca ttgaatgccg caaacgaaat caccgttgct
    36241 gcttttcttg cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg
    36301 gaaaaaatgg atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac
    36361 gcgagtgaag tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac
    36421 agagagtcgc gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg
    36481 taagtagttg gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata
    36541 ggcttcagta ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt
    36601 ctgctactca accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca
    36661 ttatggacgg caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata
    36721 aagccggggc aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgagg
    36781 cgttaacgct gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg
    36841 cgttaatgga actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata
    36901 acgtgcgtct gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg caagaacgta
    36961 ttcgtaaatc tgaagcgcta acagccggaa ataccggtct gacgctgaat attgcggcga
    37021 actacggtgg acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc
    37081 aaggaaacct gcaaccagat cagatagatg aagagatgtt aaaccagcat gtctgtatgc
    37141 atgaactggc ccctgtagat ttagtaatta ggactggggg agagcatcgc attagtaact
    37201 ttttgctttg gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt
    37261 tcgatgaaca agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg
    37321 gcggcaccga gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc
    37381 tgatatctgc ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg
    37441 tggggttcgc cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc
    37501 ttagcggttt taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt
    37561 tggcgctgat gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg
    37621 aaatctcact ttgggcttcg ttgggttggt ggattgtcgc gctattgctg gtgctgtttt
    37681 acccaggttc cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc
    37741 taaccattgt tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga
    37801 atcattacag tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact
    37861 ccggcgcata tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc
    37921 cgggtaaaac ctggcaaggc tttataggtg gactcgctac tgcagcggta atctcatggg
    37981 gttatggcat gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg
    38041 tcgcagcgtt agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag
    38101 gaattaagga cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata
    38161 gcctgacggc tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt
    38221 aacggaaggt aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg
    38281 tgtacttatc accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg
    38341 cgttgagcgt ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg
    38401 caccgaatat gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg
    38461 cgcagaaccg gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca
    38521 acgagcggcg attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta
    38581 ctggctggtt tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc
    38641 caattcgata gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg
    38701 tatcgaaacg cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga
    38761 aagcaccacc attacagtag cgccatttga cagcgaccaa cggcgggatg taaagctcga
    38821 tttacgtcac tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg
    38881 tcctcgtggg ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag
    38941 caaggcaggt ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca
    39001 gtgggtgacc tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat
    39061 cgaaaggcag gggagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg
    39121 taaagcgatt ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa
    39181 agttgtacgc cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca
    39241 gctgatgaag ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa
    39301 caacctcagt gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt
    39361 agtttattac ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt
    39421 tccgttgccc gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg
    39481 cggaccggta tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt
    39541 gctgttaatg gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa
    39601 gaacgcataa taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc
    39661 gccaccgtat acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag
    39721 cgtgtcgccg ttggtgcggc cctcctcagt atgccggtgc gtacaggcga cacggttaat
    39781 gatgaagata tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt
    39841 cgcgtccttc gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc
    39901 agcattactt tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag
    39961 gcttctggtg tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa
    40021 ggtctggaag acttctacta cagcgtcggt aaatatagcg ccagcgttaa agctgtcgtg
    40081 accccgctgc cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct
    40141 gaaatccagc aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct
    40201 catttccaac tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg taaataccag
    40261 aaacagaaac tggcgggcga tcttgaaacc ctgcgcagct actatctgga ccgcggttat
    40321 gcccgtttca acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac
    40381 gtcacggtga acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc
    40441 aaccttgccg ggcactccgc tgaaattgag caactgacta agatcgagcc gggcgagctg
    40501 tataacggca ctaaagtgac caaaatggaa gatgacatta aaaagcttct cggtcgctat
    40561 ggttatgcct atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt
    40621 aaattacgcg tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa
    40681 ggtaacgata cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca
    40741 tggttgggga gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt
    40801 gaaactgtcg ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt tgatgtagtc
    40861 tacaaggtga aagaacgtaa cactggtagc ttcaacttcg ggattggtta cggtactgaa
    40921 agtggcgtga gcttccaggc tggcgtgcaa caggataact ggttaggtac aggttatgct
    40981 gttggtatca acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac
    41041 ccgtacttca ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa tgacttccag
    41101 gcagatgacg ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg
    41161 ggcttcccga ttaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc
    41221 ctgtccaaca tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat
    41281 ccgagcacct ctgatcagga taacagcttc aaaacggacg acttcacgtt caactatggt
    41341 tggacctata acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg
    41401 accggtaaag tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg
    41461 gcgacttatg tgccgatcga tgacgatcac aaatgggttg ttctgggtcg tacccgctgg
    41521 ggttatggtg atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt
    41581 ggttccagca ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc agtttacttc
    41641 ccgcatcagg ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc
    41701 gcgaaagacc tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc
    41761 ctcgagttca tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact
    41821 tcattcttct gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct
    41881 ggatatccgg actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa
    41941 tggatgtccc cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat
    42001 ggagacaagg cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca
    42061 aaggaatgta gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca
    42121 cgcaaagaac tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg
    42181 gttattagct gcaggtcttg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat
    42241 tgcaatcgtc aatatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa
    42301 cacgctggaa aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct
    42361 gcaggctaaa atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca ccaaactgga
    42421 aaaagacgtg atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga
    42481 tcgcgcacgt cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt
    42541 gaaatccgtt gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta
    42601 caacagcagc gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat
    42661 gccttcaatt cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg
    42721 cgatatcgtc atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt
    42781 catggttaac ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat
    42841 gacccaggac gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct
    42901 gacttacgcg cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc
    42961 acccagtgcg gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa
    43021 cgcggtgatt gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt
    43081 cgtaggtaaa aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta
    43141 ccatgagatc cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga
    43201 cggctttggt tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt
    43261 aattattggc gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga
    43321 tgacactgtt attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt
    43381 cgtgattggc gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat
    43441 tggtcgttac tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga
    43501 caaagtgacg gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta
    43561 ttcctcaggc attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat
    43621 gaacattgat gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga
    43681 ctaacgttcc atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc
    43741 aggccgtgtt attattgtcg tttcttatat tttgacagga agagtatctt gactactaac
    43801 actcatactc tgcagattga agagatttta gaacttctgc cgcaccgttt cccgttctta
    43861 ctggtggatc gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc
    43921 tctgtcaatg agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg
    43981 ctgattctgg aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa
    44041 ctggaaccgg gtgagctgta ctacttcgct ggtattgacg aagcgcgctt caagcgcccg
    44101 gtcgtgcctg gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg
    44161 acccgtttta aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc aacgatgatg
    44221 tgtgctcgta gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc
    44281 gccattgtgg aagagggcgc gtcaattggc gcgaacgcac acattggtcc tttttgtatc
    44341 gttggacccc atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat
    44401 ggtcatacta aaattggccg cgataatgag atttatcagt ttgcctccat cggcgaagtt
    44461 aaccaggatt tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc
    44521 attcgcgaaa gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg
    44581 ggcagcgaca acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac
    44641 cgctgtattc tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga cgacttcgcg
    44701 atcatcggcg gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt
    44761 ggcggctgct ccggtgtggc gcaggacgtc cctccttatg ttattgcgca gggtaaccac
    44821 gcaacgccgt tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgtgaggcg
    44881 attaccgcta tccgcaatgc gtataagcta atttatcgta gcggtaaaac gctcgatgaa
    44941 gtgaaaccgg aaattgctga actggcggaa acatatccgg aagtgaaagc ctttaccgat
    45001 ttctttgcac gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt ccattaacga
    45061 ttgccctggt cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc
    45121 tgaaagaacg tgtgcccaac gcccgctttg ttggtgttgc cgggccacga atgcaggctg
    45181 aaggctgcga agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc
    45241 tcggtcgtct gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggcgaac
    45301 tgaagccaga tgtttttgtt ggtattgatg cgcccgactt caatattact cttgaaggta
    45361 acctcaaaaa gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc
    45421 gacagaaacg cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt
    45481 tcgaaaaagc gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg
    45541 ctgatgccat gccgttagat ccagataaaa atggtgcccg tgatgtgctg gggatccctt
    45601 acgatgccca ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc gaaatgctta
    45661 gtgccgattt cttgaaaact gcccagcttt tgcgccagac atatccggat ctcgaaatcg
    45721 tggtgccgct ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg
    45781 cgccagacct gtcggttcat ttgctggatg gaatgggccg tgaggcgatg gtcgccagcg
    45841 atgcggcact actggcgtcg gggacggcag ccctggagtg tatgctggcg aaatgcccga
    45901 tggtggtggg atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa
    45961 ctgattatgt ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa gagttattgc
    46021 aggaagagtg tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga
    46081 aaaccagcca cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg
    46141 ccgatgagca ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc
    46201 gcacacgcag ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt
    46261 cgtcaccgct gcggtgatcc ttgacccggc gcgcccgatt gccgggctga atgattccaa
    46321 aaagctgagc gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga aagcgttgag
    46381 ctggagtctg ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac
    46441 catgctggcg atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat
    46501 tgatggtaac cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggggatag
    46561 ccgcgtacca gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat
    46621 ggcggcgctg gatattgttt tcccgcaata tggttttgcc caacataaag ggtatccaac
    46681 cgcttttcat ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt
    46741 tgggcctgtc aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag attaagtaaa
    46801 ccggaatctg aagatgtctg aaccacgttt cgtacacctg cgggtgcaca gcgactactc
    46861 gatgatcgat ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg cgttgggtat
    46921 gccagcactg gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt tctacggagc
    46981 gggacatggc gcagggatta agcctatcgt cggggcagat tttaatgtcc agtgcgacct
    47041 gctgggtgat gagttaaccc acctgacggt actggcggcg aacaataccg gctatcagaa
    47101 tctggcgttg ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg ggccgatcat
    47161 cgatcgcgac tggcttatcg aattaaacga agggttgatc cttctttccg gcgggcgcat
    47221 gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg agtgtgtcgc
    47281 gttttatgaa gaacacttcc cggatcgcta ttttctcgag ctgatccgca ccggcaggcc
    47341 ggatgaagaa agctatctgc acgcggcggt ggaactggcg gaagcgcgcg gtttgcccgt
    47401 cgtggcgact aacgatgtgc gctttatcga cagcagcgac tttgacgcac acgaaatccg
    47461 cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa cgcccgcgta actattcgcc
    47521 gcagcaatat atgcgtagcg aagaggagat gtgtgagctg tttgccgaca tcccagaagc
    47581 ccttgccaac accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc ttggtgaata
    47641 cttcctgccg cagttcccga ccggggacat gagcaccgaa gattatctgg tcaagcgtgc
    47701 aaaagaaggc ctggaagagc gtctggcatt tttattccct gacgaggaag aacgtcttaa
    47761 acgccgcccg gaatatgacg aacgtctgga gactgaactt caggttatca accagatggg
    47821 cttcccgggc tacttcctca tcgttatgga atttatccag tggtcgaaag ataacggcgt
    47881 accggtaggg ccaggccgtg gctccggtgc gggttcactg gtggcctacg cgctgaaaat
    47941 caccgacctc gatccgctgg aatttgacct gctgttcgaa cgtttcctta acccggaacg
    48001 tgtctccatg cctgacttcg acgttgactt ctgtatggag aaacgcgatc aggttatcga
    48061 acacgtggcg gacatgtacg gtcgtgatgc ggtatcgcag attatcacct tcggtacgat
    48121 ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt acggctttgt
    48181 cgaccgtatc tcgaaactga tcccgcccga tccggggatg acgctggcga aagcgtttga
    48241 agccgagccg cagctgccgg aaatctacga agcggatgaa gaagttaagg cgctgatcga
    48301 catggcgcgc aaactggaag gggtcacccg taacgccggt aagcacgccg gtggggtggt
    48361 tatcgcgccg accaaaatta ccgattttgc gccgctttat tgcgatgaag agggcaaaca
    48421 tccggtcacc cagtttgata aaagcgacgt tgaatacgcc ggactggtga agttcgactt
    48481 ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg gagatgatca acaagcggcg
    48541 ggcgaagaat ggcgagccac cgctggatat cgccgcgatc ccgctggatg acaagaaaag
    48601 cttcgacatg ctgcaacgct cggaaaccac ggcggtattc cagcttgaat cgcgcggcat
    48661 gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg cactggtggc
    48721 actgttccgc cctggtccgt tgcagtcagg gatggtggat aactttatcg accgtaaaca
    48781 tggtcgtgaa gagatctcct atccggacgt gcagtggcag catgaaagcc tgaaatcggt
    48841 actggagcca acctacggca ttatcttgta tcaggaacag gtcatgcaga ttgcccaggt
    48901 gctttctggt tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg gtaagaaaaa
    48961 gccggaagag atggctaagc agcgttctgt atttgctgaa ggtgcagaaa agaacggaat
    49021 caacgccgaa ctggcgatga aaatcttcga cctggtggag aaattcgcgg gttacggatt
    49081 taacaaatcg cactctgcgg cctatgcttt ggtgtcatat caaacgttat ggctgaaagc
    49141 gcactatccg gcggagttta tggcggcggt aatgaccgcc gatatggaca acaccgagaa
    49201 ggtggtgggc ctggtggatg agtgctggcg gatggggctg aaaatcctgc caccagatat
    49261 aaactccggt ctttaccatt tccacgtcaa cgacgatggc gaaatcgtgt atggtattgg
    49321 cgccatcaaa ggggtaggtg aaggtccgat tgaggccatc atcgaagccc gtaataaagg
    49381 cggctacttc cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa agttaaaccg
    49441 gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc cacaccgcgc
    49501 ggcgctgatg aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg cgaaagcgga
    49561 agctatcggt caggccgata tgttcggcgt gctggccgaa gagccggaac aaattgaaca
    49621 atcctacgcc agctgccaac cgtggccgga gcaggtggta ttagatgggg aacgtgaaac
    49681 gttaggcctg tacctgaccg gacaccctat caaccagtat ttaaaagaga ttgagcgtta
    49741 tgtcggaggc gtaaggctga aagacatgca cccgacagaa cgtggtaaag tcatcacggc
    49801 tgcggggctc gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc gtatcggtat
    49861 ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg acgccctgga
    49921 taaataccag caattgctgg aaaaagaccg catacttatc gtcagcggac aggtcagctt
    49981 tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata ttgacgaagc
    50041 ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa ttgatgacca
    50101 gcttttaaac cgactccgtc agtctctgga accccaccgc tctgggacaa ttccagtaca
    50161 tctctactat cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga cgtggcgtgt
    50221 ctctccgagc gatcgtttat taaacgatct ccgtggcctc attggttcgg agcaggtgga
    50281 actggagttt gactaataca ggaatactat gagtctgaat ttccttgatt ttgaacagcc
    50341 gattgcagag ctggaagcga aaatcgattc tctgactgcg gttagccgtc aggatgagaa
    50401 actggatatt aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg tagaactgac
    50461 gcgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg caactggcac gccatccgca
    50521 gcgtccttat accctggatt acgttcgcct ggcatttgat gaatttgacg aactggctgg
    50581 cgaccgtgcg tatgcagacg ataaagctat cgtcggtggt atcgcccgtc tcgatggtcg
    50641 tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa ttcgccgtaa
    50701 ctttggtatg ccagcgccag aaggttaccg caaagcactg cgtctgatgc aaatggctga
    50761 acgctttaag atgcctatca tcacctttat cgacaccccg ggggcttatc ctggcgtggg
    50821 cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa tgtctcgcct
    50881 tggcgtaccg gtagtttgta cggttatcgg tgaaggtggt tctggcggtg cgctggcgat
    50941 tggcgtgggc gataaagtga atatgctgca atacagcacc tattccgtta tctcgccgga
    51001 aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg ctgaagcgat
    51061 gggtatcatc gctccgcgtc tgaaagagct gaaactgatc gactccatca tcccggaacc
    51121 gctgggcggc gctcaccgta acccggaagc gatggcggca tcgttgaaag cgcaactgct
    51181 ggcggatctg gccgatctcg atgtgttaag cactgaagat ttaaaaaatc gtcgttatca
    51241 gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg gtcacttcgg
    51301 tggccctttt ttatcgccac ggtttgagca ggctatgatt aaggaaggat tttccaggag
    51361 gaacacatga acatcattgc cattatggga ccgcatggcg tcttttataa agatgagccc
    51421 atcaaagaac tggagtcggc gctggtggcg caaggctttc agattatctg gccacaaaac
    51481 agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt gatttttgac
    51541 tgggatgagt acagtctcga tttatgcagc gatatcaatc aacttaatga atatctcccg
    51601 ctttatgcct tcatcaacac ccactcgacg atggatgtca gcgtgcagga tatgcggatg
    51661 gcgctctggt tttttgaata tgcgctgggg caggcggaag atatcgccat tcgtatgcgt
    51721 cagtacacca acgaatatct tgataacatt acgccgccgt tcacgaaagc cttgtttacc
    51781 tacgtcaaag agcggaagta caccttttgt acgccggggc atatgggcgg caccgcatat
    51841 caaaaaagcc cggttggctg tctgttttat gattttttcg gcgggaatac ccttaaggct
    51901 gatgtctcta tttcggtcac cgagcttggt tcgttgcttg accacaccgg gccacacctg
    51961 gaagcggaag agtacatcgc gcggactttt ggcgcggaac agagttatat cgttaccaac
    52021 ggaatatcga cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg cagtacgctg
    52081 ttgatcgacc gcaactgcca taaatcactg gcgcatctgt tgatgatgaa cgatgtagtg
    52141 ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc tgggggggat cccgcgccgt
    52201 gaatttactc gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc acaatggccg
    52261 gttcatgcgg tgatcaccaa ctccacctat gatggtttgc tctacaacac cgactggatc
    52321 aaacagacgc tggatgtccc gtcgatccac ttcgattccg cctgggtgcc gtatactcat
    52381 tttcatccaa tctaccaggg taaaagtggt atgagcggcg agcgtgttgc gggaaaagtt
    52441 ttcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc ttcactgatc
    52501 cacattaaag gcgagtatga cgaagaggcg tttaacgaag cctttatgat gcataccacc
    52561 acctcgccca gttatcccat tgttgcttcg gttaagacgg cggcggcgat gctgcgtggt
    52621 aatccgggca aacggctgat taaccgttcg gtggagcggg cgctgcattt tcgcaaagag
    52681 gtccagcggc tgcgggaaga gtctgacggt tggtttttcg atatctggca accgccgcag
    52741 gtggatgaag ccgaatgcag gcccgttgcg cctggcgaac aatggcacgg ctttaacgat
    52801 gcggatgccg atcatatgtt tctcgatccg gttaaagtca ctattttgac accggggatg
    52861 gacgaacagg gcaatatgag tgaggagggg atcccggcgg cgctggtagc aaaattcctc
    52921 gacgaacgtg ggatcgtagt agagaaaacc ggcccttata acctgctgtt tctctttagt
    52981 attggcatcg ataaaaccaa agcaatggga ttattgcgtg ggttgacgga atttaagcgc
    53041 tcttacgatc tcaacctgcg gatcaaaaat atgctgcccg atctctatgc tgaagatccc
    53101 gatttctacc gcaatatgcg tattcaggat ctggcgcaag ggatccataa gctgattcgt
    53161 aaacacgatc tttccggttt gatgttgcgg gcattcgata ctttgccgga aatgatcatg
    53221 acgccacatc aggcatggca gagacagatt aaaggtgaag tagaaaccat tgcgctggaa
    53281 caactggtcg gtagagtatc ggcaaatatg atcctgcctt atccaccggg cgtaccgctg
    53341 ttgatgccag gagaaatgct gaccaaagag agccgcacgg tactcgattt tctactgatg
    53401 ctttgttccg tagggcaaca ttaccccggt tttgaaacgg atattcacgg cgcgaaacag
    53461 gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact tgccagagcg
    53521 gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg ggtttaaaac
    53581 aggttcacca tattgcgatt attgcgacgg attatgcggt gagcaaagct ttctactgcg
    53641 atattcttgg tttcacgctg caaagcgaag tctatcgcga agcgcgcgac tcgtggaaag
    53701 gggatttggc gcttaatggg caatatgtga ttgagctttt ctcttttccg ttcccgccgg
    53761 agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc gttgatgaca
    53821 tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc atccgtgtcg
    53881 atccatacac gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg ccgttggaac
    53941 tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg ggcattgcta
    54001 ctgtaaaatc gcaccatcat gacactcacg ctcaatagac aacttctcac ctcacgccag
    54061 attctggtgg cctttagcgg cgggcttgac tccaccgttc tgctgcatca gttggtgcag
    54121 tggcggacgg aaaatccggg cgtcactctg cgcgctattc atgtgcatca cggtttaagt
    54181 gccaatgccg atgcctgggt tacgcattgc gaaaacgtct gccaacagtg gcaggtgccg
    54241 ctggtggtcg aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc ccaggcgcgg
    54301 caggcacgtt atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct ggtcaccgcg
    54361 caacatctcg acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg tagcggccct
    54421 gccgggcttt cggctatggc ggaagtttcg gagtttgctg gaacacggct tatccgcccg
    54481 ttgcttgccc gcacgcgggg ggaactggag cagtgggcat tggcgcatgg tttacgctgg
    54541 attgaagacg aaagtaatca ggacgactca tacgatcgta actttctgcg cctgcgcgta
    54601 gtgccgttat tgcagcagcg ttggccgcat tttgcggaag caacggcccg cagcgccaca
    54661 ctttgtgctg aacaagagag tctgttggat gaactgctgg cggatgattt agcacactgc
    54721 caaacgtcgc aggggacgct gcagattgcg ccaatgctgg cgatgagtga tgcccgtcgc
    54781 gcggcgatta ttcgccgctg gctggcaggg cagaatgcac cgatgccttc ccgcgacgcg
    54841 ctggtgcgca tctggcagga agtggcgctg gcgcgggaag atgcttcccc ctgtttacgt
    54901 ttgggcgcct ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa atccgtcacc
    54961 gggcaaagcg aaaccattgt gctgtggcag acgtggctac aaccgctgga attaccggcg
    55021 gggctgggaa ccgtacagct tactgcggga ggcgatattc gccctccgcg tgcagacgaa
    55081 gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg caacggcgga
    55141 cgtaagctga agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg tgacaccacg
    55201 ccactgctgt tttatggaga aacgctgatt gcggcggcag gggtatttgt gacgcaagaa
    55261 ggtgtggctg aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct tagttaagtg
    55321 aaagccggat aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg attcgcttac
    55381 caccaccgta ccgatttcag ggtggctaaa gctggtaatt ttatccagac gcagctcgcg
    55441 ggtttcgcca gcggcctcga cgaccagata ctccacattt ttgcgggaga ctaaatcact
    55501 ggcttttgcc tgcaattttt cgccatcttt cagctcaagt gtcagcatta aatgatgctg
    55561 gcaggcgagc tcaagattat cgtaatcatc acaattaatt ggttgatacg tatcattcat
    55621 tgacataatc gctcaccagt aagtttgccg cagcgtatgc tgctttttcc ctgacagcct
    55681 cagaaagggc gtcgtcggca gccatttcat tcagcacttt caaaacgcag cccagcgcgt
    55741 ccggaacgta tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt attaactcac
    55801 aatatttttc cacatgccct cctgtcagca ctctgactta accgtggatg caagtctaag
    55861 cctacgaaga taaactctgt ttcgcaaggt gactatacca cactcatttc tgcaatatca
    55921 gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga gttttcttat
    55981 ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg gccgatctcg accgcaacca
    56041 gtttctcgat gcctctctga cgctggcgcg ccacccttca gaaacccagg agcgtatgat
    56101 gctgcgcttg ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta cccgtggttt
    56161 gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat cacctgggca ttgatttgtg
    56221 gattgagctg gggctgccgg atgagcgacg aattaagaaa gcctgcaccc aggccgcaga
    56281 agtggcgctg tttacctata atagtcgggc ggcgcaaatc tggtggcagc aaaatcagag
    56341 caaatgtgtg cagtttgcca atctttccgt ctggtatctg gacgatgaac aactggcgaa
    56401 agtaagcgcc tttgccgatc gtaccatgac gctgcaggca acgattcagg atggtgttat
    56461 ctggttatcg gatgataaga ataatctgga agttaactta accgcctggc aacaaccttc
    56521 atgattgtga tttcccgaca tgttgctatt cccgatggtg agcttgagat caccgccatt
    56581 cgtgcgcagg gcgctggcgg gcagcatgtt aataagacct caacggctat tcatctgcgt
    56641 tttgacattc gggcgtccag cctgccagag tattacaaag agcgtctgct cgccgccagc
    56701 catcatttga tcagcagtga tggcgtgatt gtcattaagg cacaggaata ccgcagtcag
    56761 gaactgaacc gcgaagccgc tctggcccgg ctggtggcag tgattaaaga tttaacaaaa
    56821 gaacaaaaag cccgacgacc cacgcggccc acccgtgcat cgaaagagcg caggctggca
    56881 tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag cggtcgggaa
    56941 taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg taatcagcct
    57001 ctttactctg atgggatgta ataatcgggc cgaagtcgat acgctttctc cggcgcaggc
    57061 tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg ccgattgcga
    57121 aggaatcgaa atctctctgt tcctcgaaaa agacggaaca tgggtgatga atgagcgtta
    57181 tctcggtgct cgtgaagaac cttcctcctt cgcttcctac ggtacatggg cgcgaaccgc
    57241 tgacaagctg gtattaaccg acagcaaagg tgaaaagtca tattatcggg cgaaaggcga
    57301 tgcgctggag atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca actatacgct
    57361 ggaaccggca caatccagtt tacctatgac gccgatgacc ctgcgaggca tgtattttta
    57421 tatggctgat gcggcgacct tcactgattg cgcgaccgga aaacgtttca tggtagcgaa
    57481 taacgcagag ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa aaccggtgtt
    57541 actgtcagta gaaggtcact ttacgcttga ggctaatccg gatactggtg cgccaaccaa
    57601 agtattagcg cccgatacgg cgggtaaatt ttacccgaat aaggattgca gtagtttggg
    57661 gcagtaaacc tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct gcgcttatca
    57721 ggcctacagc atataatgca acattttgaa ttagcacgat tttgtaggcc ggataaggcg
    57781 tttacgccgc atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca
    57841 aagcgacccg ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt
    57901 tctggcttaa acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc
    57961 cagcccgatt gcgccagtgt aagtggatgc ccggatttgg caaatgcatc agtgacaaat
    58021 tccgaacaaa accacgactt tttgtctcct tcgcacacac tgcttaactg cgctttcgcc
    58081 aggtcgctga cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg
    58141 cgagtcacca taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg
    58201 attttattgg caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt
    58261 aagacgaaaa gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca
    58321 gcgcctgtcg cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag
    58381 gaagtgctga agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa
    58441 tcgccgggtt tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt
    58501 tgatgctgga atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg
    58561 tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaagcagg
    58621 cggcagtacg cctttggttt atccatttta tacaatccat gtaaaaaagg gccctgaaat
    58681 tcaggaccct ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc
    58741 agtcttaatt aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc
    58801 gaggttacgg tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa
    58861 catcacgccc ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag
    58921 ttcgctgtac agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg
    58981 cagaatcgcc acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta
    59041 gttctgctca atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt
    59101 caggatttgg ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt
    59161 ggtacccagc tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg
    59221 gccatccggg cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc
    59281 gacatcgcga tcccagttga taccgaagta gtgtttacca tcgatgttag caccagcagc
    59341 gaaatcactc atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg
    59401 acccagtgaa cccggaccgg ctttaaccac agcacgaatt tcttcttcgg tcgcgaaagt
    59461 cagcgggctt gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc
    59521 gcgcaccagc agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt
    59581 cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc
    59641 gttcggcgta tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc
    59701 gatagcttct gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc
    59761 gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc
    59821 ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc
    59881 cgcatacatt gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc
    59941 atctttcatc aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg
    60001 gaacttggtc tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt
    60061 acgaatcagg tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc
    60121 acggtcaaca aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca
    60181 caaatcggct ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc
    60241 ttcacgcacg atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata
    60301 taacccggag gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac
    60361 ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt
    60421 gttacggttc cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga
    60481 tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc
    60541 agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgtagc
    60601 cagacggcat aggttttgcc cgtttcctca cctttacgat aggccgggcg cgggtcctgc
    60661 gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc
    60721 tgcttttcga cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca
    60781 tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact
    60841 accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct gcctttatgg
    60901 caaacaacct ctttcagctc taccagcgac atgccaattg ggttagggcg gaaagtagag
    60961 cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga
    61021 cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaatgct
    61081 tccaggccgc gaacggcgtc ggcctggttg taggaagcta tgagatgcag ttcgccgttg
    61141 gcgcttttta ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga
    61201 ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc
    61261 cgatacatac agcctgacga tagcagcccg gcgtaccgct ggtgacttcg cagctatgca
    61321 gtaatacagc attggctttc attttagagg cattgatttg catctgctta cgtgcggttg
    61381 gaatgctcgg cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga
    61441 gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg
    61501 cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg
    61561 gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga
    61621 tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg
    61681 agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga atgatattac aaattgtgga
    61741 aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt
    61801 cgtaaacttc atcagactga taagcctgga cgaatttctt cacgttctcg gcgtctttgt
    61861 tatcttcacg cgttacgatc aggtttacgt atggggagtc tttatcttcg acaaagatac
    61921 cgtctttcgc cggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag
    61981 cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc ttccagttca acaattttca
    62041 gattttttgg gttctcaaca acatcaagaa cggtcggcag caggccaacg ccatctttca
    62101 gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaagatta gttggatcgt
    62161 ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt
    62221 aaccagcaat cggataaaca aaagtgttgc ctactgcgac cagtttgtag ccacgatctt
    62281 tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc
    62341 tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat
    62401 atttgtcttt cgcaactttc tgcgcgactt ctgctacctg ctgttcggca ccaacaatca
    62461 cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg
    62521 atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct
    62581 tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt
    62641 cgcctgcaaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca
    62701 ccgtcgcgtt gtagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac
    62761 cgactgcacc acccatcgcg gaataaccga ccagggtaat cagggtgata gttgccgcat
    62821 tcaccagacc cggcaacgct tccggtaaca gcactttaca gacgatctgc attggcgtgg
    62881 cacccattgc gcgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga
    62941 ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaattgct gcctgcaaac
    63001 caatcgatgt accgacaata acgcgggtaa acggaatcat ccataccagc aagataatga
    63061 acgggatgga acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag
    63121 caataatttg ccccggacgc gtgacataaa gcagaacgcc aaccggcaga ccaatcacaa
    63181 acccaaaaaa tccggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca
    63241 gccacatcat cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc
    63301 caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg
    63361 aatttaacgc caccggcgta gtccatctgc gcgctaataa tgttgttgtt gacgttgaaa
    63421 cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc
    63481 agcatcggca cgcagtcagt aaatggctcc gtttgcagac gttcctggta atcttccggg
    63541 atatccagat gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag
    63601 aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac cgccacgcaa
    63661 tcacaaatac gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga
    63721 cggcggttaa tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtccagcgcg
    63781 ctggtggctt catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc
    63841 acacgttgtt tctgcccacc ggaaagattc gacgggtagc tatcatgctt atcgccaaga
    63901 ccaaccaatg acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtgttgtcc
    63961 agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc
    64021 tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc
    64081 aattcctggc catcgaccag cacgctaccc tcggttgggc gctccagcaa gtttacacaa
    64141 cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgtccagct
    64201 ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg gtggaacact
    64261 ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtag
    64321 catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac
    64381 gtctaaatca atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact
    64441 agcgtcccat gccttattaa ggagctataa aaggtggcga agagcgtacc cgcaattttt
    64501 cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt
    64561 gaatttatcg acggtgttat tgacgccatg cgcgagctaa aaaaaatggg ctttgcgctg
    64621 gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc acagtttgaa
    64681 acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc
    64741 tattattgcc cgcatcatcc gcaggggagt gttgaagagt ttcgccaggt ctgcgattgc
    64801 cgcaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc
    64861 gcttcttata tggtgggcga taaattagaa gatatgcagg cagcggttgc ggcgaacgtg
    64921 ggaacaaaag tgctggtgcg tacgggtaaa cctattacac ctgaagcaga aaacgcggca
    64981 gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg
    65041 gcgcaatgat taaaagatga acggttgaaa taaaaatgca tttttccgct tgtcttcctg
    65101 agccgactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    65161 agccggttcg gttgaagaga aaaaatcctg aaattcaggg ttgactctga aataggaaag
    65221 tgtgatatac gccacctcgc gacagtgagc tgaaagctgc gtcgcaactg ctctttaaca
    65281 atttatcaga caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacgat
    65341 aaatgaatac caagtctcaa gagtgaacac gtaattcatt acgaagttta attctttgag
    65401 catcaaactt ttaaattgaa gagtttgatc atggctcaga ttgaacgctg gcggcaggcc
    65461 taacacatgc aagtcgaacg gtaacaggaa gcgcttgctg
//
LOCUS       DAJTYH010000009        59831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTYH010000009 DAJTYH010000000
VERSION     DAJTYH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..306
                     /locus_tag="OO609_000577"
     CDS             <1..306
                     /locus_tag="OO609_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6632112.1"
                     /translation="NGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVY
                     KDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            419..1213
                     /gene="panB"
                     /locus_tag="OO609_000578"
     CDS             419..1213
                     /gene="panB"
                     /locus_tag="OO609_000578"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6632113.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1225..2076
                     /gene="panC"
                     /locus_tag="OO609_000579"
     CDS             1225..2076
                     /gene="panC"
                     /locus_tag="OO609_000579"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6632114.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2150..3052)
                     /gene="rpnC"
                     /locus_tag="OO609_000580"
     CDS             complement(2150..3052)
                     /gene="rpnC"
                     /locus_tag="OO609_000580"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6632115.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3326..3706
                     /gene="panD"
                     /locus_tag="OO609_000581"
     CDS             3326..3706
                     /gene="panD"
                     /locus_tag="OO609_000581"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6632116.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3710..4939)
                     /locus_tag="OO609_000582"
     CDS             complement(3710..4939)
                     /locus_tag="OO609_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6632117.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5003..5443)
                     /locus_tag="OO609_000583"
     CDS             complement(5003..5443)
                     /locus_tag="OO609_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6632118.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5548..6318)
                     /locus_tag="OO609_000584"
     CDS             complement(5548..6318)
                     /locus_tag="OO609_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6632119.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6315..7241)
                     /locus_tag="OO609_000585"
     CDS             complement(6315..7241)
                     /locus_tag="OO609_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6632120.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7350..8012
                     /gene="can"
                     /locus_tag="OO609_000586"
     CDS             7350..8012
                     /gene="can"
                     /locus_tag="OO609_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6632121.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8053..8589)
                     /gene="hpt"
                     /locus_tag="OO609_000587"
     CDS             complement(8053..8589)
                     /gene="hpt"
                     /locus_tag="OO609_000587"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6632122.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8795..11185
                     /gene="gcd"
                     /locus_tag="OO609_000588"
     CDS             8795..11185
                     /gene="gcd"
                     /locus_tag="OO609_000588"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6632123.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11232..12782)
                     /gene="cueO"
                     /locus_tag="OO609_000589"
     CDS             complement(11232..12782)
                     /gene="cueO"
                     /locus_tag="OO609_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6632124.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12948..13295
                     /locus_tag="OO609_000590"
     CDS             12948..13295
                     /locus_tag="OO609_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6632125.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13401..14267
                     /gene="speE"
                     /locus_tag="OO609_000591"
     CDS             13401..14267
                     /gene="speE"
                     /locus_tag="OO609_000591"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6632126.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14283..15077
                     /gene="speD"
                     /locus_tag="OO609_000592"
     CDS             14283..15077
                     /gene="speD"
                     /locus_tag="OO609_000592"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6632127.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15115..15477)
                     /locus_tag="OO609_000593"
     CDS             complement(15115..15477)
                     /locus_tag="OO609_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6632128.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15652..18249)
                     /gene="acnB"
                     /locus_tag="OO609_000594"
     CDS             complement(15652..18249)
                     /gene="acnB"
                     /locus_tag="OO609_000594"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6632129.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVSVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18604..20457
                     /locus_tag="OO609_000595"
                     /pseudo
     CDS             18604..20457
                     /locus_tag="OO609_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20528..21952)
                     /gene="lpdA"
                     /locus_tag="OO609_000596"
     CDS             complement(20528..21952)
                     /gene="lpdA"
                     /locus_tag="OO609_000596"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6632130.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22277..24157)
                     /gene="aceF"
                     /locus_tag="OO609_000597"
     CDS             complement(22277..24157)
                     /gene="aceF"
                     /locus_tag="OO609_000597"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6632131.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24172..26835)
                     /gene="aceE"
                     /locus_tag="OO609_000598"
     CDS             complement(24172..26835)
                     /gene="aceE"
                     /locus_tag="OO609_000598"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6632132.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26996..27760)
                     /gene="pdhR"
                     /locus_tag="OO609_000599"
     CDS             complement(26996..27760)
                     /gene="pdhR"
                     /locus_tag="OO609_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6632133.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28301..29674
                     /gene="aroP"
                     /locus_tag="OO609_000600"
     CDS             28301..29674
                     /gene="aroP"
                     /locus_tag="OO609_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6632134.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29717..30571)
                     /gene="ampE"
                     /locus_tag="OO609_000601"
     CDS             complement(29717..30571)
                     /gene="ampE"
                     /locus_tag="OO609_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6632135.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30568..31119)
                     /gene="ampD"
                     /locus_tag="OO609_000602"
     CDS             complement(30568..31119)
                     /gene="ampD"
                     /locus_tag="OO609_000602"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6632136.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31207..32100
                     /gene="nadC"
                     /locus_tag="OO609_000603"
     CDS             31207..32100
                     /gene="nadC"
                     /locus_tag="OO609_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6632137.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32303..32743
                     /gene="ppdD"
                     /locus_tag="OO609_000604"
     CDS             32303..32743
                     /gene="ppdD"
                     /locus_tag="OO609_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6632138.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32753..33900
                     /gene="tadA"
                     /locus_tag="OO609_000605"
                     /pseudo
     CDS             32753..33900
                     /gene="tadA"
                     /locus_tag="OO609_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33890..35092
                     /gene="hofC"
                     /locus_tag="OO609_000606"
     CDS             33890..35092
                     /gene="hofC"
                     /locus_tag="OO609_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6632139.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35127..36170)
                     /gene="guaC"
                     /locus_tag="OO609_000607"
     CDS             complement(35127..36170)
                     /gene="guaC"
                     /locus_tag="OO609_000607"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6632140.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36332..36370)
                     /locus_tag="OO609_000608"
     CDS             complement(36332..36370)
                     /locus_tag="OO609_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632141.1"
                     /translation="MIIKTLKMSARK"
     gene            36395..37015
                     /gene="coaE"
                     /locus_tag="OO609_000609"
     CDS             36395..37015
                     /gene="coaE"
                     /locus_tag="OO609_000609"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6632142.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37015..37758
                     /gene="zapD"
                     /locus_tag="OO609_000610"
     CDS             37015..37758
                     /gene="zapD"
                     /locus_tag="OO609_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6632143.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37768..37965
                     /gene="yacG"
                     /locus_tag="OO609_000611"
     CDS             37768..37965
                     /gene="yacG"
                     /locus_tag="OO609_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6632144.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38065..38454)
                     /gene="mutT"
                     /locus_tag="OO609_000612"
     CDS             complement(38065..38454)
                     /gene="mutT"
                     /locus_tag="OO609_000612"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6632145.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38514..41219)
                     /gene="secA"
                     /locus_tag="OO609_000613"
     CDS             complement(38514..41219)
                     /gene="secA"
                     /locus_tag="OO609_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6632146.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41281..41793)
                     /gene="secM"
                     /locus_tag="OO609_000614"
     CDS             complement(41281..41793)
                     /gene="secM"
                     /locus_tag="OO609_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6632147.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42024..42941)
                     /gene="lpxC"
                     /locus_tag="OO609_000615"
     CDS             complement(42024..42941)
                     /gene="lpxC"
                     /locus_tag="OO609_000615"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6632148.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43042..44193)
                     /gene="ftsZ"
                     /locus_tag="OO609_000616"
     CDS             complement(43042..44193)
                     /gene="ftsZ"
                     /locus_tag="OO609_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6632149.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44254..45516)
                     /gene="ftsA"
                     /locus_tag="OO609_000617"
     CDS             complement(44254..45516)
                     /gene="ftsA"
                     /locus_tag="OO609_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6632150.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45513..46343)
                     /gene="ftsQ"
                     /locus_tag="OO609_000618"
     CDS             complement(45513..46343)
                     /gene="ftsQ"
                     /locus_tag="OO609_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6632151.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46345..47265)
                     /gene="ddlB"
                     /locus_tag="OO609_000619"
     CDS             complement(46345..47265)
                     /gene="ddlB"
                     /locus_tag="OO609_000619"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6632152.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47258..48733)
                     /gene="murC"
                     /locus_tag="OO609_000620"
     CDS             complement(47258..48733)
                     /gene="murC"
                     /locus_tag="OO609_000620"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6632153.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48787..49854)
                     /gene="murG"
                     /locus_tag="OO609_000621"
     CDS             complement(48787..49854)
                     /gene="murG"
                     /locus_tag="OO609_000621"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6632154.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49851..51095)
                     /gene="ftsW"
                     /locus_tag="OO609_000622"
     CDS             complement(49851..51095)
                     /gene="ftsW"
                     /locus_tag="OO609_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6632155.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51095..52411)
                     /gene="murD"
                     /locus_tag="OO609_000623"
     CDS             complement(51095..52411)
                     /gene="murD"
                     /locus_tag="OO609_000623"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6632156.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52414..53496)
                     /gene="mraY"
                     /locus_tag="OO609_000624"
     CDS             complement(52414..53496)
                     /gene="mraY"
                     /locus_tag="OO609_000624"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6632157.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53490..54848)
                     /gene="murF"
                     /locus_tag="OO609_000625"
     CDS             complement(53490..54848)
                     /gene="murF"
                     /locus_tag="OO609_000625"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6632158.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54845..56332)
                     /gene="murE"
                     /locus_tag="OO609_000626"
     CDS             complement(54845..56332)
                     /gene="murE"
                     /locus_tag="OO609_000626"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6632159.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56319..58085)
                     /gene="ftsI"
                     /locus_tag="OO609_000627"
     CDS             complement(56319..58085)
                     /gene="ftsI"
                     /locus_tag="OO609_000627"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6632160.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSVLVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58101..58466)
                     /gene="ftsL"
                     /locus_tag="OO609_000628"
     CDS             complement(58101..58466)
                     /gene="ftsL"
                     /locus_tag="OO609_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6632161.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58463..59404)
                     /gene="rsmH"
                     /locus_tag="OO609_000629"
     CDS             complement(58463..59404)
                     /gene="rsmH"
                     /locus_tag="OO609_000629"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6632162.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59406..>59831)
                     /gene="mraZ"
                     /locus_tag="OO609_000630"
     CDS             complement(59406..>59831)
                     /gene="mraZ"
                     /locus_tag="OO609_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6632163.1"
                     /translation="SKGRLSVPTRYREQLLENAAGQMVCTIDIHHPCLLLYPLPEWEI
                     IEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHAGLTKEVMLVGQ
                     FNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14296 a        16610 c        14726 g        14199 t
ORIGIN      
        1 aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg agtcggtgta
       61 ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc taatgattca
      121 aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg aggagtttac
      181 ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct acaacaggat
      241 ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca ggtaacctac
      301 ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata aatcattgcc
      361 cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag gatacgttat
      421 gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc gtttcgcgac
      481 catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc ttaacgtcat
      541 gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc tgccagtaac
      601 cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa actgcctgct
      661 attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg aaaacgccgc
      721 aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt ggctggtcga
      781 aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag gtttaacccc
      841 acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg aagcgagcga
      901 tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc tggtgctgga
      961 atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc cggttattgg
     1021 tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg cctttggtat
     1081 taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg gcgacatccg
     1141 cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg gcgaagaaca
     1201 cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct gctgcgtcag
     1261 caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac tatgggtaat
     1321 ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga tgtggtcgtc
     1381 gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc acgttatcca
     1441 cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt agttttcgcc
     1501 ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt tgacgttcct
     1561 ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg cgtttcgact
     1621 attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg tgaaaaagat
     1681 tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga tattgagatt
     1741 gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg taacggttat
     1801 ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag ttcgattgct
     1861 gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc ggggcaagaa
     1921 ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga cacattgctg
     1981 gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct tggcgatgct
     2041 cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc tacggctgat
     2101 ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact taaataaggt
     2161 taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg tcttcccgag
     2221 acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt cttccctgct
     2281 gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagacttcc
     2341 ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc
     2401 gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa
     2461 tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca
     2521 gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga
     2581 tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc gagtaaaaca
     2641 tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg
     2701 gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg gcagcaatag
     2761 aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt tcaatcacaa
     2821 catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc gtgctgtgac
     2881 ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg agatcacaaa
     2941 gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcatgca
     3001 ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc atcttccctg
     3061 tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc gtcagccacc
     3121 tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg cgttacatgg
     3181 cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag tagacactca
     3241 tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc tgcttcgtta
     3301 acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg
     3361 cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt
     3421 tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg tcaccaacgg
     3481 caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa
     3541 cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca gcttcgttac
     3601 catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg aaggcgacaa
     3661 tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg
     3721 gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag atataaagtc
     3781 gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca
     3841 ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg ccaaacggat
     3901 acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat
     3961 caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa ccatctaccc
     4021 gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagctcag
     4081 aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt ttgatgcgtg
     4141 aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga tacgcatagc
     4201 ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga
     4261 tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat cccctgtcgc
     4321 gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa gtatggcgaa
     4381 aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc aggccattat
     4441 cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc
     4501 aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca
     4561 gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta tctttccagg
     4621 taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct tcaacttttt
     4681 gtcgtccctg aactggctcg agatgccctt tatcgataaa ccctttacca aagccaaaat
     4741 taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg gcccgaatat
     4801 tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt tcgatggttt
     4861 gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc agcaggagaa
     4921 taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt tattgtagca
     4981 aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg acgaaaggcg
     5041 gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt tcgacaatac
     5101 actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt tcaattaacg
     5161 gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat aatgaagcca
     5221 cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca ccagagtccg
     5281 cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat ccgcgccaga
     5341 aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca tccagtatct
     5401 cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc ctccttagcc
     5461 gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa actcctctcc
     5521 cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac gttggatcag
     5581 cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa aggtagtgac
     5641 cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca tataaacgat
     5701 tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg agtaaaagac
     5761 cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga tgtcatcgaa
     5821 cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg ccgtgagcac
     5881 cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa acagtgaaat
     5941 tgccgtcaca agaatgccaa caaacagacc acgcgccacg ccaccgccga catatccggc
     6001 aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac gctggaactt
     6061 ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg acatcatgat
     6121 cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa tacgcgaacc
     6181 tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg gcaccagcgt
     6241 ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt ttagcgccac
     6301 ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga aacaaacagc
     6361 tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg ctcacttaac
     6421 tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag cgtcgcggta
     6481 tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg tgcgagatcg
     6541 agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt attttccacc
     6601 agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc ttccaggtag
     6661 tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt
     6721 gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag tagtttaggt
     6781 tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga taacatacgc
     6841 gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc gctgcggatg
     6901 tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat ttgctgcacg
     6961 gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg tttagcgttc
     7021 acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt tttatttacc
     7081 agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc gagaagcgca
     7141 taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac gccgcctgga
     7201 tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta cttaccttac
     7261 gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt agccaaacgt
     7321 aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc atcagcaaca
     7381 atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag aaactggcac
     7441 aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt cctgcagaac
     7501 gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct aacctagtca
     7561 ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta ctcgaagttg
     7621 aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc gttgaaaacc
     7681 cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg ttcaaacata
     7741 gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt gaactgaacg
     7801 tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg tggaaacgcg
     7861 ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg ctgcgtgatc
     7921 tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca
     7981 acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc aacacatccg
     8041 gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca gatgacggta
     8101 acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg agaaaccgat
     8161 aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg tacaaatcgc
     8221 caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg tattccccga
     8281 gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat ccagatcttt
     8341 gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg cggtcataaa
     8401 gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca taaatgagcc
     8461 acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct cagtaatctg
     8521 acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta cttctacagt
     8581 atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg gcaggttttg
     8641 gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt cgcggaatct
     8701 gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa ttaattgcta
     8761 ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata cagcctcgcg
     8821 acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt atctactcat
     8881 tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg ctggccttgt
     8941 gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt ggctatacgc
     9001 agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg acttctgggc
     9061 gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc tgccgtttgt
     9121 ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg tcgcactgct
     9181 gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga tcaacggcac
     9241 cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc aggactggcc
     9301 tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa ttcacgccga
     9361 taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga agcagccgaa
     9421 cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata ccctttacct
     9481 gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag agaaatggca
     9541 ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc gtggcgtctc
     9601 ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt gcccgcgtcg
     9661 tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa acggcaaact
     9721 gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc cagacaccaa
     9781 accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca tcgtgatggc
     9841 cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga
     9901 tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc cgaacgcaat
     9961 cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac cagcggccta
    10021 tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg atatctgggg
    10081 cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc tgaatgccac
    10141 taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg acatggatct
    10201 tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc cgattattta
    10261 cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg aactggtggt
    10321 tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg ttacccctac
    10381 tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg cggatatgtg
    10441 gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga
    10501 aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta acctggggat
    10561 gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg ccaacccaat
    10621 ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga tggagcagcc
    10681 gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg gtgtaccgta
    10741 tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac agccagcatg
    10801 gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga aacgtattgg
    10861 tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca atatgggtat
    10921 gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg ccgctacggc
    10981 agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc agggtcgttt
    11041 accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc agtacgtggt
    11101 gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata ttgtggctta
    11161 tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc atatttccga
    11221 atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg ctccagcaga
    11281 tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt caccagcact
    11341 tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg agccgctggc
    11401 ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa cggatgcagc
    11461 atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc cgccgcaaac
    11521 atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg gaaatcgaac
    11581 ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat catctggctg
    11641 tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg catccccatc
    11701 atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc ttccagcgaa
    11761 ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact aatagcaatc
    11821 ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat ccccatctgg
    11881 ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac cagcacttca
    11941 aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg tagcagacca
    12001 ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt gagcgagcgg
    12061 gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc gtgttgtggg
    12121 tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc ggtcatcaca
    12181 tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac gatcaccgga
    12241 acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc atcttcaatc
    12301 accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg ttgatgtgga
    12361 tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg cttgccacct
    12421 ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag cccgtgccag
    12481 tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc tttgccgcgc
    12541 tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcagttttc
    12601 tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg ggcatcggtc
    12661 gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc acggctccac
    12721 agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc acgacgttgc
    12781 atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc atagtccttc
    12841 cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg
    12901 ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt
    12961 tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac gcgctcagcg
    13021 agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt
    13081 gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc
    13141 aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc ggtgaagaca
    13201 gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa gccctggccc
    13261 gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata
    13321 ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt
    13381 aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc
    13441 agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccagg
    13501 atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac
    13561 aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg
    13621 cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg
    13681 aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg
    13741 tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac gatccgcgct
    13801 ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag acctttgatg
    13861 tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc acctcagcat
    13921 tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca caaaacggcg
    13981 tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca
    14041 gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg
    14101 catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt
    14161 ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg
    14221 ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga gaagataaga
    14281 aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta
    14341 tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt gcttatatcg
    14401 atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg
    14461 gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc agcgtcacta
    14521 ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa caccccggcc
    14581 cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta catacctacc
    14641 cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa gtctctacct
    14701 gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt gagtccgata
    14761 tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt atgaagcact
    14821 ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt
    14881 atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag atgttgctta
    14941 aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta accgacagcg
    15001 agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca
    15061 atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat ttatttactt
    15121 ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag
    15181 cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc
    15241 cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt attcatgccc
    15301 tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc
    15361 aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc
    15421 catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt aatccatagt
    15481 gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg taattatcgc
    15541 agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa tgcccgggaa
    15601 tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc
    15661 agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt tgaagttcag
    15721 ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct ggtactcttc
    15781 cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag aagccaggaa
    15841 gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg aaaccaccgt
    15901 cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg ggatctcgat
    15961 acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt gcgcggcgtc
    16021 catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt gcgcatccag
    16081 cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga taaatacttc
    16141 gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat cgttcggagc
    16201 acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg cgtattccgc
    16261 atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg
    16321 gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat
    16381 gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac aaccagcggc
    16441 agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca gatccggcag
    16501 accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct tctcaacggt
    16561 cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc gcagggtgat
    16621 gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat
    16681 tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag agataccgat
    16741 cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca gcatacggtt
    16801 cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat
    16861 gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg
    16921 gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc
    16981 atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt tcggttcgca
    17041 gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tctgcgccag
    17101 cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg
    17161 cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca gcggaatacg
    17221 gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag
    17281 ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc
    17341 catgttcagg ttagagacgc tgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt
    17401 aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac
    17461 atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt tacgcgatga
    17521 acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc
    17581 aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt cacgggcgtt
    17641 tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat ccggtgccgg
    17701 agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg tcagtttttc
    17761 agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca ttacctgctt
    17821 cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag
    17881 cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat ccagcgcgtc
    17941 gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga tagctttttc
    18001 cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga agcctgcttt
    18061 tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat ctaacaggaa
    18121 ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca tttggtttgc
    18181 atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc
    18241 ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac
    18301 gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca
    18361 ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg
    18421 ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata
    18481 atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact
    18541 cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga
    18601 attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg cagtgccggg
    18661 ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc gcctgtagaa
    18721 ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc tgcaacggtt
    18781 tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc acaaatagat
    18841 caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt gctgatggca
    18901 tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa
    18961 caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca
    19021 ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac
    19081 ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg
    19141 caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt tattaccaca
    19201 acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc atccaatacc
    19261 gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc cattgagccc
    19321 gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg
    19381 gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttgttgac
    19441 agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc actattcagc
    19501 agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga tgattatcat
    19561 caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga caacatcaat
    19621 atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca
    19681 tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag
    19741 cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc tgctccaaca
    19801 cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc
    19861 gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaacac
    19921 tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac ccagcagcaa
    19981 aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca ggcagtccgc
    20041 gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg ccagcgtatt
    20101 cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac taacccgcag
    20161 aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc cgaacagcgc
    20221 caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg tgataacgcc
    20281 cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga gaaagttcag
    20341 gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa taacgagcaa
    20401 cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga aaggtaaatt
    20461 gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg ttccggcaaa
    20521 cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca
    20581 cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga
    20641 tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc agctcgccgc
    20701 cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg aaaatcagct
    20761 tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc cacgggaagg
    20821 tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg
    20881 caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag tagtgtttct
    20941 taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg
    21001 gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt
    21061 caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt
    21121 taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagctg
    21181 gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa
    21241 ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga
    21301 tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac
    21361 ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc attaccagca
    21421 ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt
    21481 catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcattgtcga
    21541 agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc ccggtgaatt
    21601 tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg
    21661 tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt
    21721 cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt
    21781 gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt
    21841 aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac
    21901 ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct
    21961 ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt
    22021 cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc gcttgctacc
    22081 ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt
    22141 tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat
    22201 ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt
    22261 gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga
    22321 tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa
    22381 tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact
    22441 tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac
    22501 ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct
    22561 taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt
    22621 tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac
    22681 cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc gaactattga
    22741 agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga
    22801 tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca
    22861 gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc cagttacggc
    22921 tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg atttcgccaa
    22981 acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg ccagtcgccg
    23041 ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca
    23101 ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca aactcgcgtg
    23161 ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca aattcagatt
    23221 tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc gctgccgctt
    23281 cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    23341 aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    23401 gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac tgttcagcgg
    23461 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    23521 ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc ggagtcgctt
    23581 cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca
    23641 gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg gtacctgcaa
    23701 acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc gactgttcag
    23761 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    23821 tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt
    23881 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    23941 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
    24001 tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg gtgatcagcg
    24061 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    24121 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    24181 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    24241 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc
    24301 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    24361 acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    24421 catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga tatacggaac
    24481 gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac catcacgcgc
    24541 cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    24601 gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca gctgaacttt
    24661 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    24721 accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac
    24781 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga cagcaacttc
    24841 gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct
    24901 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
    24961 gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc cgatacgctg
    25021 gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt tcgtgctgta
    25081 agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag
    25141 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
    25201 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    25261 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    25321 cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct gctcttccag
    25381 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
    25441 gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat gctcttcaga
    25501 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    25561 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
    25621 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    25681 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    25741 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
    25801 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    25861 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    25921 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
    25981 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    26041 aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt cacgggtagc
    26101 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    26161 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    26221 ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact gccagaattc
    26281 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    26341 gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt aaacgcccgg
    26401 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    26461 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatgtggcc
    26521 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
    26581 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    26641 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
    26701 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    26761 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    26821 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    26881 gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta gttaccgcgc
    26941 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    27001 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cggcttctgt
    27061 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    27121 gcttaccgac cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    27181 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    27241 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
    27301 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
    27361 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
    27421 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag
    27481 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
    27541 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
    27601 cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga
    27661 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
    27721 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
    27781 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
    27841 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
    27901 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gtaggaaagg
    27961 gattttttaa cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt
    28021 tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga gaggggttga
    28081 ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg
    28141 gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc
    28201 aaacctcctt gatgtaaaca aatcaataca acaaacggaa ttgcaaactt acacacgcat
    28261 cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca
    28321 cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg
    28381 cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg cagggccagg
    28441 gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg
    28501 tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg
    28561 gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc
    28621 catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac
    28681 ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa
    28741 agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg tagtagcgat
    28801 gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag
    28861 caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat
    28921 ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc
    28981 tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc gcatcctgat
    29041 tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc
    29101 cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc
    29161 gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa
    29221 cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc tggcgtctgt
    29281 cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg
    29341 cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt
    29401 atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc
    29461 caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg
    29521 gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc
    29581 gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa
    29641 agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca cccgtacagg
    29701 gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca atcaccacca
    29761 cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat
    29821 cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc tgatattgcg
    29881 aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca ccatgaccga
    29941 tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcactg
    30001 catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc
    30061 atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca
    30121 gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct
    30181 cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc
    30241 cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat
    30301 aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca
    30361 gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacaccca
    30421 tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga
    30481 acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa
    30541 ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa
    30601 ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg
    30661 gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc
    30721 aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc
    30781 aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc
    30841 acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc
    30901 ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc
    30961 agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg
    31021 caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg gtgagggaac
    31081 gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg
    31141 tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt
    31201 tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct gctggaacgc
    31261 attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca
    31321 gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc
    31381 acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga agaggtgttt
    31441 attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc
    31501 aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa
    31561 cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat
    31621 gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc
    31681 ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg
    31741 ctttctgatg ccttcctgat caaagaaaac catattattg tctccggctc agtgcgccag
    31801 gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga agtagagaat
    31861 ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct ggataacttc
    31921 gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg
    31981 tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc
    32041 tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa
    32101 ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt
    32161 cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg
    32221 ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa agtagcacca
    32281 accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat
    32341 ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt atcaaaacta
    32401 cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc gtaccgccgt
    32461 agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat
    32521 tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc
    32581 gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag gttgggataa
    32641 cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca
    32701 gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat
    32761 tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg ccagcgaaga
    32821 agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg cattgcattt
    32881 cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggtcacgc
    32941 cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc ccaaagcaga
    33001 gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata ttcatatcga
    33061 accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc
    33121 ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc tgggaaacct
    33181 ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa
    33241 caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag
    33301 gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc agccgttaca
    33361 actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc
    33421 tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa ttccaccact
    33481 gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt
    33541 acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg ccgacagcaa
    33601 ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca ctggcaggca
    33661 cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt tgaagtcctg
    33721 cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga atcgctggaa
    33781 acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtggag
    33841 caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca tggcaagtaa
    33901 gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg
    33961 ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta ccccactaag
    34021 cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat
    34081 tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc tggctctgct
    34141 ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct
    34201 cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat ttccgccgct
    34261 ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga
    34321 actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat cagcgttgcg
    34381 ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt
    34441 tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca
    34501 ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg tgttgttcgg
    34561 ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc ttatcgcgcg
    34621 gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac aaaaactcac
    34681 gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt tacagggcgt
    34741 agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca
    34801 gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg gtgagtttag
    34861 cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg acctcatgtt
    34921 agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc
    34981 cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc
    35041 aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg
    35101 tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt ttcttgttcc
    35161 agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc cccaacgtat
    35221 gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac ctggcctcgc
    35281 agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc gccaacgtga
    35341 cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc
    35401 tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa
    35461 tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc accgtcgctg
    35521 acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc agaaagctgc
    35581 ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact
    35641 ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg
    35701 ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag
    35761 tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg gttcaggtcg
    35821 agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac catcacatgt
    35881 ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac agaaaagtgt
    35941 ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga aaatgtgcct
    36001 acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc tgaatgtttg
    36061 aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg
    36121 cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc
    36181 ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa
    36241 tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc
    36301 tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt tttaacgttt
    36361 tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg ccttaacggg
    36421 aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt
    36481 cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc
    36541 cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc agcgccgggc
    36601 cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg acctgctgca
    36661 tccgctgatt caacaagaga cgcaacacca gatccagcaa gcaacttccc cctatgtact
    36721 gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt
    36781 ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg
    36841 cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc
    36901 agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct
    36961 gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag
    37021 acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag
    37081 tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat
    37141 ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag
    37201 ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc
    37261 gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt gagcgtatta
    37321 atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt
    37381 cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg
    37441 ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac
    37501 ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt
    37561 aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc
    37621 ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt
    37681 gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc
    37741 gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc
    37801 tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt
    37861 tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc
    37921 gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc
    37981 tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga
    38041 tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta ccggttcatt
    38101 ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac cgggttgccc
    38161 ttctttaccc cacggccccc cttcccaact ttcgaccagc cagaaccaca gagttatatg
    38221 cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc
    38281 gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca tttcaatttt
    38341 accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac gtgttataaa
    38401 gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt tcattgtctt
    38461 aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag cttttattgc
    38521 aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg atcgttacgt
    38581 cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga gtcgtcatcc
    38641 tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg ctgttgttcc
    38701 agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact gataacttca
    38761 tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt gtattcctgc
    38821 ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata gtccatcgct
    38881 gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt ctcgaagtga
    38941 cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat ggactgcgcc
    39001 agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag ccactcggta
    39061 attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg aatatcccac
    39121 atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa cacatcttca
    39181 cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt acgctgggag
    39241 taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg cttacgaatg
    39301 tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt cacccacggg
    39361 tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga tactcggtcg
    39421 gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga agaaccagca
    39481 tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga ttcgtgacgc
    39541 tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg tacctgccag
    39601 tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc
    39661 tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt cgcgatagtc
    39721 accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg gaatttggcg
    39781 ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag ctccgatttt
    39841 tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc tttgatatct
    39901 tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg cagatcttta
    39961 cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat ggagctaaat
    40021 tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata cagacggaag
    40081 tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg cacaccttct
    40141 ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat ggtacgaccg
    40201 gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac gtcacgggta
    40261 aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat gttggccgga
    40321 gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc ttcaatcagc
    40381 accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga gaagtggcct
    40441 tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg aataatttta
    40501 ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat cagcggtgta
    40561 cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg cagtttacgc
    40621 tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa gccgtattcg
    40681 ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg tgctggcatg
    40741 cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg gttgttttcg
    40801 gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt accggttagt
    40861 gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt acgcatttcg
    40921 gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa gtgacgcata
    40981 ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat cagattttcc
    41041 agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt cagttcttca
    41101 tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac tttgcgcatc
    41161 cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt gattagcata
    41221 ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg tttattgttg
    41281 ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc agacgggcgt
    41341 gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct tttcagacgg
    41401 cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg
    41461 aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga aagaaagatg
    41521 acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt tcgaattcgg
    41581 gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc
    41641 gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc tgagcgcagg
    41701 caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt agcgtttacc
    41761 aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat cgaattcaaa
    41821 cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga atgaagataa
    41881 gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag ctcttataaa
    41941 attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg ccagtcgaat
    42001 tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt gaaggccaac
    42061 ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt cgccaggaca
    42121 gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa tgcaccaata
    42181 atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg acgcacaaat
    42241 tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac aacgatggca
    42301 caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat atcacgcatg
    42361 aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga gaagttcatc
    42421 gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt gaaatccagc
    42481 gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac acgaacagtc
    42541 tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc gtcaagcagc
    42601 aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc gttaacttcg
    42661 ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc tacggttgaa
    42721 atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg cacagatttg
    42781 gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat gaccccggtg
    42841 ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa accgacaccc
    42901 gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc tcgccaaatt
    42961 acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa agagcctcga
    43021 aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg ggatatccag
    43081 ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag caaccggctt
    43141 ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca ctggctgctg
    43201 aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga tacctgtcgc
    43261 aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac cgataaccac
    43321 agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga actcatccag
    43381 acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag acaggtcgat
    43441 atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac ggtcttcacc
    43501 gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg tgcgtacgtc
    43561 tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac cttgcacagc
    43621 gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac cgcggcccag
    43681 aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct tggacagttc
    43741 agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga aaggcttagt
    43801 gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga ctggtgctgc
    43861 acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg caccttccag
    43921 cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt ctggattagc
    43981 gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc caaccgctgt
    44041 tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt caatacgctc
    44101 gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga ctttaatcac
    44161 cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg tgcctgtcgc
    44221 ctgagtccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca gccaactatt
    44281 gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt cagcttcacc
    44341 gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat aatacggctc
    44401 ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca cttgcgtatg
    44461 aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac cggttaatac
    44521 aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct gcaactgcaa
    44581 tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca cctctgccag
    44641 ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt ccacgctctc
    44701 atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg cttcggcgtc
    44761 gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc cagcataagg
    44821 aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca ttgtaccacc
    44881 accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata ccgaataact
    44941 tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt caaccgcttt
    45001 gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg cctgcatccg
    45061 cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg catactcttg
    45121 cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg cggtatggac
    45181 gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca taccaatttc
    45241 attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga tctgacaatc
    45301 tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg attcgaggtc
    45361 gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa tgatattgac
    45421 cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac caatttccag
    45481 tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg tgcctgattt
    45541 tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc tccagagtca
    45601 taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg ctgtaaaacc
    45661 ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc aagattgagc
    45721 ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc cgcttccttc
    45781 agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg caacacttca
    45841 ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt gcgatctggc
    45901 ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca ccgcgcaatc
    45961 ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct gacgctcacc
    46021 tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac atcctgggtc
    46081 ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc attacgcgtg
    46141 taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc atcttccatc
    46201 cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag caggaaaagg
    46261 atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc gctgtttcgc
    46321 gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta ctaccaactg
    46381 cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc tggtcatacc
    46441 cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca taacgtcaat
    46501 acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg cctgcaaatt
    46561 ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat cagagagata
    46621 cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg gtaaaatttc
    46681 ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa tcaatacttc
    46741 ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg ctactacttt
    46801 tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca aacccagagc
    46861 agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc gggttaacgc
    46921 tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc gtagtttatc
    46981 tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca tcagctcgag
    47041 catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa acactttctg
    47101 aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag gatacgcatc
    47161 aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag aaacttcccg
    47221 ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt tcttcctccg
    47281 gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg atattaccag
    47341 ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc tcggctacct
    47401 gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt gtgcgacaca
    47461 gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact tccagcatca
    47521 acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg cgcgtacggg
    47581 taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct gcgcgcgccg
    47641 ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc agcattgccg
    47701 taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca aaacggcgac
    47761 cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt ccctcttccg
    47821 tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc gcattcaggg
    47881 tgacgcacat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc gggccaatct
    47941 gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt gtggtctgac
    48001 gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc accgcacgac
    48061 cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc tcaaagtcgc
    48121 cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc atcggttgca
    48181 gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga ccatgcccca
    48241 aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt gggtcgagcc
    48301 ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg tgcgttcctg
    48361 caatggcgat gccatgacga aaacgcatta actcagccag catttcggca cgacggatca
    48421 ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa atggcgctgg
    48481 aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa taaatcgtcg
    48541 cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa ccactgatct
    48601 gataaccttc attggccaga acttcggcaa taccgcccat accggcacca ccaatgccga
    48661 caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt tttgccaatt
    48721 gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca aaaggcatcg
    48781 ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg tggcatccgg
    48841 aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg accacccggc
    48901 cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt tggctgcgcc
    48961 cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt gaaacggcac
    49021 aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac cggagcggca
    49081 aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt cagtcacttt
    49141 atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg aacctttgcc
    49201 gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg gcattgtctg
    49261 gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg gaccttcacg
    49321 tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca ccgggttacc
    49381 caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg tagcaatctt
    49441 cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta caaccggaat
    49501 gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca taccgagtac
    49561 cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt tgaagatacg
    49621 cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga tacgaatgaa
    49681 atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg cagtccccag
    49741 ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc ccgggaatac
    49801 atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac tcatcgtgaa
    49861 cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg caacagcatc
    49921 atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag cggcaatgtc
    49981 agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc ctggaagcta
    50041 aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat ttctaatgct
    50101 ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag tgctagtacc
    50161 acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt gtgcgcttcc
    50221 ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag ttcgccgcga
    50281 ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc ttcccacggg
    50341 ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag caacacaacc
    50401 gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc cgccaggaac
    50461 aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg ctgtgccagc
    50521 agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg caggttatta
    50581 cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag cgacagtttt
    50641 gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga tgccccttta
    50701 acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag cagcatcgtg
    50761 gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc cagaataaac
    50821 gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt ggttaagcgt
    50881 tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc gaggccgaag
    50941 gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt ttcccgcgag
    51001 cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa tcctggcagg
    51061 cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc gccagacggg
    51121 caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca caggccgggg
    51181 agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata gcctgttcca
    51241 tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg ccgtcacgac
    51301 cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg ctaaagtccg
    51361 ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc aggccattca
    51421 gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag cgcacaccgt
    51481 tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt aacgctttca
    51541 ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc agcgcattgg
    51601 tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc tcgcctttaa
    51661 cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg ttgacgccaa
    51721 agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca tcagcattaa
    51781 ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac tgttgtaaac
    51841 caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct gctaccgcct
    51901 gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac agttcacact
    51961 catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca acgttaaccc
    52021 ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg ttagaaccgg
    52081 taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg ccaacgattt
    52141 cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata ccgggactgg
    52201 cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg tggcgttcta
    52261 cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata acgcgcggcg
    52321 tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc aggccgataa
    52381 tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg ttgccagacc
    52441 aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg gttccggcca
    52501 gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt gtccgcgcag
    52561 tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca cgccccccat
    52621 aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg caccacctaa
    52681 cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa accacaggaa
    52741 gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg cgtgtcgcag
    52801 atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg ccaccagcgc
    52861 aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac catcggtcag
    52921 gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt agaacagccc
    52981 cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg gcgtgtcttt
    53041 gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc agaaatattt
    53101 ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat catcaacaaa
    53161 gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg gattggacgg
    53221 gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc cgcccatggt
    53281 cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta cctgaccaaa
    53341 ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga tgaacagcgc
    53401 ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa agccggaata
    53461 atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg taaagcgcgt
    53521 actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat tacctgttgc
    53581 tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa atgttcgcca
    53641 acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac gcggtcaata
    53701 ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc agcgcccagt
    53761 tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag tacctgaaca
    53821 gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa ctggttttct
    53881 gccagttgga tggggaacag acggccagga acagctttca gatttgccag ccccgctttg
    53941 atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag cgcattcgca
    54001 atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg ggtttgtagg
    54061 gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc gctgttggcg
    54121 gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca gttcagccag
    54181 tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct aaagatttca
    54241 cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc cgctgccagg
    54301 ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat ctcgccctga
    54361 tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg caacagcgtc
    54421 atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt gccgcactgg
    54481 cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc cgtcagagca
    54541 accacgagcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa cgccagacgc
    54601 gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac cagtagtgcg
    54661 cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg ttcacctttc
    54721 agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac agcatcaagg
    54781 gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct aagggttacg
    54841 ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag tagtccagac
    54901 gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt accacatcat
    54961 tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc atcactttgg
    55021 catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc ggttcttcgg
    55081 tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca attgcgccca
    55141 tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac cacagcttgc
    55201 ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc gtatgcgcgt
    55261 aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt ccgcaaaccg
    55321 gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc gccagcagtg
    55381 ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg ctttcaattt
    55441 cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga tagttcactt
    55501 cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc attgataccg
    55561 caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg gcgttaataa
    55621 tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg tagtgttcca
    55681 tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac gccggaaatt
    55741 tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt gcgcaaaaag
    55801 tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc gaaccggtag
    55861 tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc attaccgcgc
    55921 tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg gttttgccgt
    55981 tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa aagcggcccg
    56041 ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg ccgtgcattt
    56101 cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca gccacacctt
    56161 gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact acagctacaa
    56221 agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt gctcgcgaag
    56281 gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc acctgtcccc
    56341 tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc cggctcgatg
    56401 ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga aacggcgccg
    56461 ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa gcgcggctga
    56521 ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg accgtccggc
    56581 ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat cgccgccttc
    56641 acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt gcgaacaatg
    56701 gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat cgacagtggg
    56761 cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa cggtgttacc
    56821 attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg ttgtttttga
    56881 ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc cagtccaaaa
    56941 cgtgagtaag tatctactaa cactgaggac ggcatcgtta acgccagctt ggaaacaccg
    57001 acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg tgccacgtct
    57061 ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt ttcccgcacc
    57121 acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc cggttcaaac
    57181 acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag attgttaggg
    57241 ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc caccagcacg
    57301 gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg ataaaccagc
    57361 gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct gtcagtagaa
    57421 gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc acccggctgc
    57481 ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact atcgacgtta
    57541 gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg agactcttca
    57601 cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc agagtttacc
    57661 tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc agaaagctga
    57721 tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac gctgataccg
    57781 ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac gctcaccgct
    57841 aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg ctgaacgcga
    57901 agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg taaccacgct
    57961 acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa cgcaaaacgc
    58021 caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt cgccgctgct
    58081 ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga cggatcaaca
    58141 tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc gccgagcgca
    58201 ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc cagcaccagt
    58261 tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac ggtcaccgcc
    58321 gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa aagatcgtca
    58381 ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt aactttgctt
    58441 agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc agaactgaac
    58501 tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg cctagtgctc
    58561 gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac cctgccggaa
    58621 cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct tccagcgagt
    58681 ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag ctttttagcg
    58741 cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg aaggtacggg
    58801 tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact tccgccagtt
    58861 ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg gcaatgcgtt
    58921 tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct tcttctgcgg
    58981 tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg tccagcggac
    59041 catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa gagacgccaa
    59101 gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg tattcgccca
    59161 gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc ttcgcaacgg
    59221 cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca agctgcgaga
    59281 ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg ccatcaggac
    59341 ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta tagttttcca
    59401 tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc caactgctct
    59461 gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag ctcaaacttg
    59521 ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg cagtactggc
    59581 gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc taacagtagg
    59641 cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt ttgctcgata
    59701 atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc aatggtgcaa
    59761 accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg cactgataag
    59821 cgccctttgc t
//
LOCUS       DAJTYH010000010        57871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTYH010000010 DAJTYH010000000
VERSION     DAJTYH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..117)
                     /locus_tag="OO609_000631"
     CDS             complement(<1..117)
                     /locus_tag="OO609_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6632164.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQ"
     gene            272..844
                     /gene="yeiP"
                     /locus_tag="OO609_000632"
     CDS             272..844
                     /gene="yeiP"
                     /locus_tag="OO609_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6632165.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1067..2533
                     /locus_tag="OO609_000633"
     CDS             1067..2533
                     /locus_tag="OO609_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6632166.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2651..3637
                     /gene="yeiR"
                     /locus_tag="OO609_000634"
     CDS             2651..3637
                     /gene="yeiR"
                     /locus_tag="OO609_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6632167.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3676..4389
                     /gene="lpxT"
                     /locus_tag="OO609_000635"
     CDS             3676..4389
                     /gene="lpxT"
                     /locus_tag="OO609_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6632168.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4801..5367
                     /gene="mepS"
                     /locus_tag="OO609_000636"
     CDS             4801..5367
                     /gene="mepS"
                     /locus_tag="OO609_000636"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6632169.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5548..7104
                     /locus_tag="OO609_000637"
     CDS             5548..7104
                     /locus_tag="OO609_000637"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6632170.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7186..9000
                     /locus_tag="OO609_000638"
     CDS             7186..9000
                     /locus_tag="OO609_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6632171.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9001..10095
                     /locus_tag="OO609_000639"
     CDS             9001..10095
                     /locus_tag="OO609_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6632172.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10095..11120
                     /locus_tag="OO609_000640"
     CDS             10095..11120
                     /locus_tag="OO609_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6632173.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11122..12711
                     /gene="yejF"
                     /locus_tag="OO609_000641"
     CDS             11122..12711
                     /gene="yejF"
                     /locus_tag="OO609_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6632174.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12715..13059)
                     /locus_tag="OO609_000642"
     CDS             complement(12715..13059)
                     /locus_tag="OO609_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632175.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13392..14582)
                     /gene="bcr"
                     /locus_tag="OO609_000643"
     CDS             complement(13392..14582)
                     /gene="bcr"
                     /locus_tag="OO609_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6632176.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14610..15305)
                     /gene="rsuA"
                     /locus_tag="OO609_000644"
     CDS             complement(14610..15305)
                     /gene="rsuA"
                     /locus_tag="OO609_000644"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6632177.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15455..17215
                     /locus_tag="OO609_000645"
     CDS             15455..17215
                     /locus_tag="OO609_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6632178.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17340..17624
                     /gene="rplY"
                     /locus_tag="OO609_000646"
     CDS             17340..17624
                     /gene="rplY"
                     /locus_tag="OO609_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6632179.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17763..18770)
                     /gene="yejK"
                     /locus_tag="OO609_000647"
     CDS             complement(17763..18770)
                     /gene="yejK"
                     /locus_tag="OO609_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6632180.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18952..19179
                     /locus_tag="OO609_000648"
     CDS             18952..19179
                     /locus_tag="OO609_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6632181.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19199..20959
                     /gene="pbgA"
                     /locus_tag="OO609_000649"
     CDS             19199..20959
                     /gene="pbgA"
                     /locus_tag="OO609_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6632182.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21034..21110
                     /locus_tag="OO609_000650"
     tRNA            21034..21110
                     /locus_tag="OO609_000650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21068..21070,aa:Pro,seq:ggg)
     gene            complement(21213..23804)
                     /gene="yejO"
                     /locus_tag="OO609_000651"
     CDS             complement(21213..23804)
                     /gene="yejO"
                     /locus_tag="OO609_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6632183.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24124..24771
                     /gene="narP"
                     /locus_tag="OO609_000652"
     CDS             24124..24771
                     /gene="narP"
                     /locus_tag="OO609_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6632184.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24982..26034)
                     /gene="ccmH"
                     /locus_tag="OO609_000653"
     CDS             complement(24982..26034)
                     /gene="ccmH"
                     /locus_tag="OO609_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6632185.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26031..26588)
                     /gene="dsbE"
                     /locus_tag="OO609_000654"
     CDS             complement(26031..26588)
                     /gene="dsbE"
                     /locus_tag="OO609_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6632186.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26585..28528)
                     /gene="ccmF"
                     /locus_tag="OO609_000655"
     CDS             complement(26585..28528)
                     /gene="ccmF"
                     /locus_tag="OO609_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6632187.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28525..29004)
                     /gene="ccmE"
                     /locus_tag="OO609_000656"
     CDS             complement(28525..29004)
                     /gene="ccmE"
                     /locus_tag="OO609_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6632188.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29001..29210)
                     /gene="ccmD"
                     /locus_tag="OO609_000657"
     CDS             complement(29001..29210)
                     /gene="ccmD"
                     /locus_tag="OO609_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6632189.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29207..29944)
                     /gene="ccmC"
                     /locus_tag="OO609_000658"
     CDS             complement(29207..29944)
                     /gene="ccmC"
                     /locus_tag="OO609_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6632190.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29986..30648)
                     /gene="ccmB"
                     /locus_tag="OO609_000659"
     CDS             complement(29986..30648)
                     /gene="ccmB"
                     /locus_tag="OO609_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6632191.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30645..31262)
                     /gene="ccmA"
                     /locus_tag="OO609_000660"
     CDS             complement(30645..31262)
                     /gene="ccmA"
                     /locus_tag="OO609_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6632192.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31281..31883)
                     /gene="napC"
                     /locus_tag="OO609_000661"
     CDS             complement(31281..31883)
                     /gene="napC"
                     /locus_tag="OO609_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6632193.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31893..32342)
                     /gene="napB"
                     /locus_tag="OO609_000662"
     CDS             complement(31893..32342)
                     /gene="napB"
                     /locus_tag="OO609_000662"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6632194.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32339..33202)
                     /gene="napH"
                     /locus_tag="OO609_000663"
     CDS             complement(32339..33202)
                     /gene="napH"
                     /locus_tag="OO609_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6632195.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33189..33884)
                     /gene="napG"
                     /locus_tag="OO609_000664"
     CDS             complement(33189..33884)
                     /gene="napG"
                     /locus_tag="OO609_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6632196.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33891..36377)
                     /gene="napA"
                     /locus_tag="OO609_000665"
     CDS             complement(33891..36377)
                     /gene="napA"
                     /locus_tag="OO609_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6632197.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36374..36637)
                     /gene="napD"
                     /locus_tag="OO609_000666"
     CDS             complement(36374..36637)
                     /gene="napD"
                     /locus_tag="OO609_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6632198.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36627..37121)
                     /gene="napF"
                     /locus_tag="OO609_000667"
     CDS             complement(36627..37121)
                     /gene="napF"
                     /locus_tag="OO609_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6632199.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37230..37394
                     /locus_tag="OO609_000668"
     CDS             37230..37394
                     /locus_tag="OO609_000668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632200.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37529..38017
                     /gene="eco"
                     /locus_tag="OO609_000669"
     CDS             37529..38017
                     /gene="eco"
                     /locus_tag="OO609_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6632201.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38139..39170)
                     /locus_tag="OO609_000670"
     CDS             complement(38139..39170)
                     /locus_tag="OO609_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6632202.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39404..39763)
                     /locus_tag="OO609_000671"
                     /pseudo
     CDS             complement(39404..39763)
                     /locus_tag="OO609_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39981..41624)
                     /gene="yojI"
                     /locus_tag="OO609_000672"
     CDS             complement(39981..41624)
                     /gene="yojI"
                     /locus_tag="OO609_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6632203.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41700..42350)
                     /gene="alkB"
                     /locus_tag="OO609_000673"
     CDS             complement(41700..42350)
                     /gene="alkB"
                     /locus_tag="OO609_000673"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6632204.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42350..43414)
                     /gene="ada"
                     /locus_tag="OO609_000674"
     CDS             complement(42350..43414)
                     /gene="ada"
                     /locus_tag="OO609_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6632205.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43488..44543)
                     /gene="apbE"
                     /locus_tag="OO609_000675"
     CDS             complement(43488..44543)
                     /gene="apbE"
                     /locus_tag="OO609_000675"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6632206.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44655..45776)
                     /gene="ompC"
                     /locus_tag="OO609_000676"
     CDS             complement(44655..45776)
                     /gene="ompC"
                     /locus_tag="OO609_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6632207.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46514..49186
                     /gene="rcsD"
                     /locus_tag="OO609_000677"
     CDS             46514..49186
                     /gene="rcsD"
                     /locus_tag="OO609_000677"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6632208.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49203..49853
                     /gene="rcsB"
                     /locus_tag="OO609_000678"
     CDS             49203..49853
                     /gene="rcsB"
                     /locus_tag="OO609_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6632209.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50053..52902)
                     /gene="rcsC"
                     /locus_tag="OO609_000679"
     CDS             complement(50053..52902)
                     /gene="rcsC"
                     /locus_tag="OO609_000679"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6632210.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53177..53953)
                     /locus_tag="OO609_000680"
     CDS             complement(53177..53953)
                     /locus_tag="OO609_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6632211.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53958..55607)
                     /locus_tag="OO609_000681"
     CDS             complement(53958..55607)
                     /locus_tag="OO609_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6632212.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55608..56167)
                     /locus_tag="OO609_000682"
                     /pseudo
     CDS             complement(55608..56167)
                     /locus_tag="OO609_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56326..>57871)
                     /locus_tag="OO609_000683"
     CDS             complement(56326..>57871)
                     /locus_tag="OO609_000683"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6632213.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14163 a        15618 c        14210 g        13880 t
ORIGIN      
        1 ctgttcatca agctgaatgc agggcgtatt ggcgggcttg ccatctggca taccgggaat
       61 agggctggaa attgaagggg cagtgcaaca cgccccgcaa cccggacggc attccatacg
      121 ttgttctctt aaggttaacc accggattga tgagcgcgca cagtaccacc ttttttgcac
      181 cagcaaaagt gcgaatacca cttgcccgaa aggcccgtcg cgagtacttt gtcgcgatat
      241 ttttgacatt ttcgactaca ggaatttttc gatgccaaga gcgaacgaaa ttaaaaaagg
      301 tatggtactg aattacaacg gcaaactgct gctggctaag gatattgata ttcagtcgcc
      361 cactgcccgc ggtgccgcta cgctgtacaa aatgcgtttt tctgatgtcc gtaccgggct
      421 gaaagtagaa gagcgtttca aaggtgatga tatcgttgac accgtgacgc tgacccgccg
      481 ttacgttgac ttctcctatg tcgatggcaa cgaatatgtc tttatggata aagaagacta
      541 taccccgtat accttcacca aagatcagat tgaagaagag ttgctgttta tgccagaggg
      601 cggcatgccg gacatgcagg tgctgacctg ggacggtcaa ctgctggcgc ttgagctgcc
      661 gcagaccgtt gatctggaaa tcgttgaaac cgcaccaggc atcaaagggg catctgccag
      721 cgcccgtaac aaaccggcga cattgagcac tggtctggtg attcaggtac cagaatactt
      781 aagcccgggc gaaaaaattc gtatccatat cgaagaacgc cgttatatgg ggcgtgctga
      841 ctaacttcag ccgcatgcag aaaagggata gctcaggctg tccctttttt aatttattat
      901 accaatcttc tattttgcgc ttcctgaaca ccttacgccc tcaattttca ctcgttgatt
      961 gatcgccctc acacttcatc gcattaacaa tccagaccaa tttcaattgc tgtcatataa
     1021 ctttacactg tcattgttaa tttatcgtta ctaagacgtg actcctatga agacaattgc
     1081 ctccgttacg ctcccgcatc atgtacacgc tccacgctac gatcgccagc agttgcaatc
     1141 acgtatcgtt cattttggct ttggcgcctt tcaccgcgct catcaggcgt tactgaccga
     1201 tcgtgtgctg aatgcccagg gcggcgactg ggggatctgt gaaatcagct tgttcagcgg
     1261 tgatcaactg atgagccagc tccgcgcaca gaaccattta tataccgtgc tggagaaagg
     1321 tgcggacggc aatcaggtga taattgtcgg tgccgttcac gaatgcctta atgcaaaact
     1381 ggattcctta gcggcaatta ttgagaaatt ttgcgagcca caggtggcaa ttgtttccct
     1441 gacgattacc gaaaaaggct attgtattga cccggccacc ggtgcactcg acaccagtaa
     1501 tccgcggatt attcacgatc tacaaacccc tgaagaacct cactccgcac cgggtattct
     1561 cgtcgaagca ctgaaacgcc gccgtgagcg cagccttaca ccgtttaccg tgctctcctg
     1621 cgacaatatt cccgacaatg gccatgtggt gaaaaacgcg gtgctgagaa tggcagaaaa
     1681 acgttcgcca gaactcgccg ggtggataaa agaacacgtc agttttccgg gaaccatggt
     1741 cgaccgcatt gttccggctg caaccgacga atcactggca gaaatcagcc agcatctggg
     1801 ggtaaatgat ccctgcgcga ttagctgcga accgtttatc cagtgggtgg ttgaagataa
     1861 cttcatcgct gggcgtcctg cctgggaagt cgcaggtgta caaatggtga atgatgtcct
     1921 gccatgggaa gagatgaaac tgcgtatgct taatggcagc cactcttttc tcgcttatct
     1981 gggttacctc tcaggattcg cctatatcag tgattgcatg caggatcgcg tatttcgcta
     2041 tgctgccaga acattaatgc tggatgagca agcgccgaca ctgcaaatta aagatgtcga
     2101 tttaacacaa tatgcggata agttaattgc acgttttgct aatccgtcgc tgaaacataa
     2161 gacctggcaa atcgcgatgg atggcagcca gaaattaccg caacgtatgc tggcaggtat
     2221 tcgcatacat ctggggcgtg aaacggactg gtcgttgctg gcattaggcg ttgctggctg
     2281 gatgcgttac gtcagcggcg ttgatgatgc cggaaatgcc attgatgttc gcgatccgct
     2341 tagcgataaa attcgcgaac ttgttgcggg cagcagcagt gaacaacgcg taaccgccct
     2401 gctttccctg cgtgaagttt tcggtgatga tctgccagat aacccgcatt ttgtgcaggc
     2461 catcgaacaa gcctggcaac aaatcgtaca attcggcgca catcaggcgc tattaaacac
     2521 cctcaaaatt taacgatttc tgcggttaaa gcggatgaag ctcaccttcg tccgctctcc
     2581 ccttctcttt tctgcctttt ttagccagga ttaacgctca gttaacttac cagagtattg
     2641 cggagccgtt gtgaccagga ccaacctcat caccggtttt ctcggcagcg ggaaaaccac
     2701 gtcgattctt catctgttag cccataaaga tcccaacgaa aaatgggcgg tactggttaa
     2761 tgaatttggg gaagtcggaa ttgatggtgc tttgctcgcc gatagcggcg cattgctgaa
     2821 agagatcccc ggcggctgca tgtgctgcgt taatggttta cccatgcagg tagggttgaa
     2881 taccttactg cgtcagggaa aaccagaccg cttgttgata gagccgaccg ggctgggcca
     2941 tccgaaacag atcctcgatc tgttaaccgc accagtctat gaaccgtgga tagatctgcg
     3001 cgccaccttg tgcattctcg atccacgcct gctgctggac gaaaaaagcg ccagcaatga
     3061 aaacttccgt gaccagctgg ctgccgcaga catcattgtc gccaataaat ccgaccgtgc
     3121 gacgcccgaa agtgagcaag cgctacagcg ttggtggcag caaaatggtg gcgatcgaca
     3181 attaattcac agtgagcatg ggaaagttga cggtcatctt ctggatttgc cgcgtcgcaa
     3241 tttagccgag ttgcccgcca gcgccgcgca ttctcatcag catgtcgtga aaaaagggtt
     3301 agcagcgtta agcctgccag agcatcaacg ctggcgtcgc agtctgaaca gcgggcaagg
     3361 atatcaggcc tgcggctgga tattcgacgc tgatacggta ttcgacacca ttggcattct
     3421 ggaatgggcg cgacttgcac cggtggaacg cgtcaaaggc gtgctgcgta ttcccgaagg
     3481 gctggtacga atcaaccgtc agggcgatga cctgcacatt gaaacgcaaa acgttgcgcc
     3541 accggacagc cgtattgagc tgatttccag cagcgaagct gactggaatg ccttgcagag
     3601 cgcgctgttg aagcttcgtt tagcgactac cgcgtaaggt tgcctgcgtt tttcagtaag
     3661 ataattagag aaaatatgat taaaaatttg ccgcaaatag tgttgttgaa tattgtcggc
     3721 cccgcgctgt ttctttcctg gtatattccc gttaatcatg gattctggtt gccgattgat
     3781 gcggatattt tttatttctt taatcagaaa ctggtcgaaa gtaaggcctt tttatggctg
     3841 gttgcattga ctaacaatcg cgccttcgac ggttgttcac tgctggcgat gggtatgttg
     3901 atgctgagtt tctggctgaa agaaaacgcc cctggcagac gacgtatcgt gattatgggt
     3961 ctggtcatgc tattaactgc agtggtatta aaccagctgg gtcaggcatt aattcctgta
     4021 aaacgggcca gcccaacatt gacttttacc gatattaacc gcgtcagcga actgctctct
     4081 gttcccacga aagatgcctc acgagatagc tttcccggcg atcacggcat gatgctgctt
     4141 attttttcgg cattcatgtg gcgttatttc ggcaaagttg caggccttat cgcccttatt
     4201 atttttatgg tttttgcatt tcccagagta atgattggcg cacactggtt tactgacatc
     4261 attgtcggtt cgatgaccgt gatattgatc ggtttgccct gggtgttgct gacgccatta
     4321 agtgatcgat taatcacctt ttttgacaaa tcactaccag gaaaaaacaa acatttccaa
     4381 aacaaataac tcacagtaat taacatcatc agggttattt ttatagtgag gataatcctg
     4441 atgatgcgca ccgtgctttc atctatcgaa cgcaaaaatc attctctaag taaatgaatg
     4501 gattgcatgc gtttcactca attgtacttt aattgaccaa ccccgcttat taactttctg
     4561 tatcactttt tcttataaaa aatcatgtaa aaccgctcgc caagaccgca ccaatcgggt
     4621 aatctcgaac tcgttttgcc tcggcggtag attatcctca cagcatataa ttttgtgcgt
     4681 tagtccacag atttggcctt aaggaattgt ttcaacatgc ccaggtaatt agtctcgtgt
     4741 cgcttggcat ttttttataa cgatatttgt cgttaaggac ttcaagggaa aacaaacaac
     4801 atggtcaaat ctcaaccgat tttgagatat atcttgcgcg ggattcccgc gattgcagta
     4861 gcggttctgc tttctgcatg tagtgcaaat aacaccgcaa agaatatgca tcctgagaca
     4921 cgtgcagtgg gtagtgaaac atcatcactg caagcttctc aggatgaatt tgaaaacctg
     4981 gttcgtaatg tcgacgtaaa atcgcgaatt atggatcagt atgctgactg gaaaggcgta
     5041 cgttatcgtc tgggcggcag cactaaaaaa ggtatcgatt gttctggttt cgtacagcgt
     5101 acattccgtg agcaatttgg cttagaactt ccgcgttcga cttacgaaca gcaggaaatg
     5161 ggtaaatctg tttcccgcag taatttgcgt acgggtgatt tagttctgtt ccgtgccggt
     5221 tcaacgggac gccatgtcgg tatttatatc ggcaacaatc agtttgtcca tgcttccacc
     5281 agcagtggtg ttattatttc cagcatgaat gaaccgtact ggaagaagcg ttacaacgaa
     5341 gcacgccggg ttctcagccg cagctaataa accgtttgga tgcaatccct tggctatcct
     5401 gacgagttaa ctgaaagcac tgcttaggca gtgctttttt gttttcattc atcagagaaa
     5461 atgatgtttc cgcgtcttga tccaggctat agtccggtca ttgttatctt ttaaatgttg
     5521 tcgtaatttc aggaaattaa cggaatcatg ttcatacgcg ctcccaattc tggacgtaag
     5581 ctcctgctta cctgcattgt agcaggcgtg atgattgcga tactggttag ctgccttcag
     5641 tttttagtgg cctggcataa gcacgaagtc aaatacgaca cactgattat cgacgtacaa
     5701 aagtatctcg atacctattt tgccgacctg aaatccacta ctgaccggct tcagccgctg
     5761 accttagata cctgccagca ggctaacccc gaactgaccg ctcgcgcggc gtttagcatg
     5821 aatgtccgta cgtttgtgct ggtgaaagat aaaaaaacat tctgttcatc tgcgactggt
     5881 gagatggaca ttccactcaa tgaattgatt ccggcgctcg acattaataa aaacgtcgat
     5941 atggcgatct tacccggcac gccgatggtg ccgaacaaac ccgcaatcgt catctggtat
     6001 cgcaaccctt tgctgaaaaa tagcggtgtg tttgccgctc tgaatctcaa cctgacgcct
     6061 tctctctttt atagttcacg gcaggaagat tacgatggcg tcgccctcat tattggcaat
     6121 actgcgctat ctaccttttc ttcacgtttg atgaacgtta atgaattaac cgacatgccg
     6181 gtccatgaaa ctaaaattgc gggcattcct ctgaccgttc ggctttatgc ggatgactgg
     6241 acatggaacg atgtgtggta cgcattttta ctgggcggca tgagtggaac tgtcgttggc
     6301 ctgctttgct attacctgat gagcgtacgt atgcgccccg acagagaaat catgaccgcc
     6361 atcaagcgcg aacaatttta cgtggcgtat caaccggtgg tggatacaca agctttgcgg
     6421 gtaacgggcc tggaagtact gctacgctgg cggcatcctg tagcgggaga aattcccccg
     6481 gatgccttca ttaactttgc cgaatcgcaa aagatgattg tgccgctgac tcagcacctg
     6541 tttgagttaa ttgcccgcga tgccgcagaa ttagaaaaag tactgccggt aggcgtcaaa
     6601 tttggcatta acattgcgcc ggaccatctg cacagcgaaa gctttaaagc agatatccag
     6661 aaactgctca cttccctgcc cgcacaccat ttccagattg tgctggaaat taccgagcgc
     6721 gatatgctga aagagcaaga agccacacaa ctcttcgcct ggctgcactc ggtcggcgta
     6781 gaaattgcta ttgatgactt cggcaccggg cacagcgcgc ttatctatct tgagcgtttt
     6841 acgctcgatt atctgaaaat tgaccgtgga tttatcaacg ccatcggtac ggaaacgatc
     6901 acttcacccg tacttgacgc ggtgctgacg ctggcgaaac gtctcaatat gctgacagtt
     6961 actgaagggg tcgaaacgcc ggaacaagcg cgatggctaa gcgaacgcgg cgttaatttc
     7021 atgcaaggct actggattag tcacccgtta ccgctggacg attttgttcg ctggctgaag
     7081 aaaccgtata cgccgcagtg gtaaggtgtg cttacgtccc ttattattca tagtgaaagc
     7141 atgccggatt gcggctaatg atgagtaaaa ggaaatccgt tgcagatgat tgtgcgcata
     7201 ctgctgctgt ttatcgctct gttcaccttc ggtgcgcagg cgcaggctat caaggaaagc
     7261 tatacctttg ccgtgctggg cgaaccccgg tacgcgttta atttcaacca ttttgattat
     7321 gtgaaccccg ccgcgccaaa aggtgggcag ataacgttgt cagccctcgg taccttcgat
     7381 aatttcaacc gctatgcact gcgcggcaac ccgggcgcac gcaccgagca gctgtacgac
     7441 acgttattta cgacttccga tgacgaacca ggcagttatt acccgctgat tgctgaaagt
     7501 gcacgctatg ctgacgatta ttcctgggtg gaggtcgcta ttaatccgcg cgcccgtttt
     7561 catgatggtt ctcccattac tgcccgcgat gtagagttta cttttcaaaa atttctgacc
     7621 gaaggcgtgc cgcaatttcg tctggtctac aaaggcacca ccgtcaaagc cattgcgccg
     7681 ttaaccgtgc gcattgagtt agctaaaccc ggcaaagaag atatgttgag tctgttttcg
     7741 ctgccggtat ttccagaaaa gtactggaag gatcacaaac ttagcgaccc gctcgccacg
     7801 cctccgcttg ccagtggtcc gtaccacgtt acgtcctgga aaatggggca aaatattgtc
     7861 tattcccgcg tgaaagatta ctgggcagca aacttaccgg taaaccgtgg acgctggaat
     7921 ttcgacacca ttcgctacga ttattacctc gatgataatg tcgcctttga agcgtttaaa
     7981 gcaggtgcct ttgatttgcg tatggaaaac gacgctaaaa actgggccac gcgttatacc
     8041 ggtaaaaatt tcgataaaaa atacatcatc aaagatgagc aaaagaacga atcagcccag
     8101 gatacgcgct ggctggcgtt taatatccaa cgtgcggtat tcagcgatcg ccgggtgcgg
     8161 gaagcaatca ctctcgcctt tgactttgaa tggatgaaca aggcgttgtt ttacaacgcc
     8221 tggagtcgca cgaacagtta ttttcaaaat accgaatacg cggccagaaa ttaccccgac
     8281 gccgcggagc tggtgcttct ggcaccaatg aaaaaagatc taccgccaga agtcttcaca
     8341 caaatctacc agccgccggt atctaaaggc gatggctacg atcgtgacaa cctgttaaaa
     8401 gccgacaaac ttctcaacga agcgggctgg gtgctgaagg gtcagcaacg cgttaatgcc
     8461 acaacgggtc agccgctcag ctttgaatta ttgcttcccg caagcagcaa tagtcagtgg
     8521 gtattgccgt tccagcacag cctgcaacgg ctgggtatca atatggacat tcgcaaggtg
     8581 gataactcgc aaatcactaa ccgcatgcgc aatcgcgact atgacatgat gccgcgacta
     8641 tggcgggcga tgccgtggcc cagttccgat ttacagattt cctggtcatc ggaatatatc
     8701 aattccactt ataatgcccc cggcgtgcaa agcccggtta tcgactcact gatcaaccaa
     8761 attattgccg cgcagggaaa taaagaaaaa ttactgccgt tggggcgagc actggatcgc
     8821 gtattaacgt ggaattatta catgctgcca atgtggtaca tggcggaaga ccgtctcgcc
     8881 tggtgggata aattctccca acccgctgta cgccctgttt acagcctggg tatcgatacc
     8941 tggtggtatg acgttaataa agcggccaaa ctgccgtcag ccagacaaca gggagagtag
     9001 atgggcgctt acctgattcg ccgtctgttg ctggtgatcc caacgttatg ggctattatc
     9061 actatcaact ttttcatcgt gcaaattgcg cctggcggtc cggtcgatca ggccatcgcc
     9121 gccattgagt ttggtaatgc cggagtatta cccggcgcag gcggtgaagg tgttcgtgcc
     9181 agccatgcgc aaacgggtgt cggcaatatc agcgacagta attaccgtgg cggacgcgga
     9241 ttagatccag aagtgatcgc tgagatcact catcgctacg gtttcgataa gccgatccac
     9301 gaacgttact tcaaaatgct ctgggactac atccgctttg attttggtga tagcctgttt
     9361 cgcagcgcct cggtgctgac gctgattaaa gacagtctgc cggtttccat caccctcgga
     9421 ttgtggagca cgctgattat ctatctggtg tcgattccgt taggcattcg caaagctgtt
     9481 tataatggga gtcgctttga cgtctggagt agcgcattta tcatcatcgg ctacgccatt
     9541 ccggcctttt tgtttgccat cctgctgatt gtcttcttcg cgggcggcag ctatttcgac
     9601 ctgttccctc tacgcggcct ggtttccgct aactttgatt cgctgccgtg gtatcagaaa
     9661 atcaccgatt atctgtggca tatcacgcta ccggtgctgg cgacagtgat tggtggcttt
     9721 gcggcgctga ccatgctgac caaaaactca ttccttgatg aagtgcgcaa gcaatacgtg
     9781 gtgaccgcgc gtgcgaaagg ggtaagtgag aaaaacattc tctggaaaca tatgttccgc
     9841 aacgccatgc tgctggtgat tgccggtttt ccggcgacgt ttatcagcat gttttttacc
     9901 ggctcgctgc tgattgaggt gatgttttca ctcaatggtc ttggcttact gggctacgaa
     9961 gcgaccgtct cgcgcgatta tcctgtaatg tttggtacct tgtatatttt caccctgatt
    10021 ggcctgctgc tgaatattgt cagtgatatc agctatacgc tggttgatcc gcgtatagat
    10081 tttgagggac gttaatgtcg cgactcagcc ccgtcaatca ggcccgttgg gcgcgttttc
    10141 gtcataaccg tcgcggctac tggtcgttat ggattttcct cgttttgttt ggtttgagtt
    10201 tgtgttctga acttatcgct aacgataaac cgttgctggt gcgttatgac ggcagttggt
    10261 atttcccgtt gttgaaaaac tacagcgaaa gcgattttgg cggcccgctg gcaagtcagg
    10321 ctgattatca ggacccgtgg ctgaaacaac ggctggaaaa taacggctgg gtactgtggg
    10381 caccgattcg ctttggtgct accagtatca actttgctac cgataagccc ttcccttctc
    10441 caccctcccg gcaaaactgg ctgggaacgg atgcaaacgg cggcgatgtg ctggcacgca
    10501 ttctctatgg cacgcggatc tcggttctgt ttggcctgat gctgactctc tgttccagcg
    10561 tgatgggcgt gctggcgggg gcgctacaag gctattacgg cggtaaagtc gatctctggg
    10621 ggcaacgctt tattgaagta tggtcgggga tgccgacgct gtttttgatt attttacttt
    10681 ccagcgtcgt acagcctaac ttctggtggc tgctggcaat tactgtcttg tttggctgga
    10741 tgagtctggt cggcgtggtg cgggcggagt ttttacgtac tcgtaatttc gactacattc
    10801 gtgcggcaca ggcgcttggc gtcagcgatc gcagtatcat cctgcgtcat atgttgccta
    10861 atgccatggt cgcgaccctc acctttttac cgtttatttt atgtagttcg ataaccaccc
    10921 tgacctcgct cgatttcctc ggcttcggtc taccgctcgg ttcaccgtca ctcggtgaac
    10981 tgctgttaca agggaaaaat aaccttcagg ccccgtggct tgggatcacc gccttcttgt
    11041 cggtggcgat attattgtct ttgctgatct ttattggtga agccgtccgc gacgcatttg
    11101 atcctaataa ggcggtgtag catgacgcaa actctgttag cgattgaaaa tttgtcggtg
    11161 ggttttcgcc atcagcaaac cgtacgtaca gtagtcaatg atgtttcact acagattgag
    11221 gctggcgaaa cgctggcgct ggtgggtgag tcaggttcag gcaaaagcgt taccgcgctg
    11281 tcaattttac gcctgctccc ttccccgccg gttgaatatc tctccggcga tattcgtttt
    11341 catggcgaat cgctgcttca tgccagcgat caaacgttgc gcggtgtacg cggtaataag
    11401 atcgccatga tttttcagga accgatggtg tcgttaaatc cattgcatac cctggaaaaa
    11461 cagctttatg aagtgctttc actccaccgc gggatgcgtc gggaagcggc tcgtggcgaa
    11521 attcttaact gccttgatcg cgtcggtatc cgccaggcgg caaaacggct gacagattat
    11581 ccgcatcagc tctccggcgg cgaacgccag cgggtgatga ttgcgatggc gctgttaacg
    11641 cgaccggaat tattaattgc cgatgaaccg accaccgcac tggacgtctc tgtccaggcg
    11701 cagattttac agctgttgcg cgaactgcaa ggcgagctga atatgggcat gctgtttatt
    11761 actcataacc tcagcattgt cagaaaactg gcccaccgcg tggcggtaat gcaaaacggt
    11821 cgctgtgtcg agcaaaataa cgccgctacg ctatttgcct cacccactca tccttacaca
    11881 caaaagctac tcaacagtga accgtcaggc gatccggtac cgttgccaga acctgcctca
    11941 acgttgctgg atgttgaaca gcttcaggtt gccttcccca ttcgcaaagg gattttgaag
    12001 cgcattgtgg atcataatgt ggtggtgaaa aacatcagtt ttacgctacg ggcgggtgaa
    12061 acactgggtt tagtgggcga gtctggttcc gggaaaagca cgacgggact ggcgctgctg
    12121 cgactgatta cctctcaggg cagcatcgtc tttgacggtc agccactgca agatttaaat
    12181 cgccgccagc tgttacctac tcgtcatcgt attcaggtgg tatttcagga tccaaactcc
    12241 tcgctcaacc cacgactcaa cgttttgcag attattgagg aaggcttacg ggttcaccag
    12301 ccgacgcttt ctgccgcaca acgcgaacaa caagtgatag ccgtgatgca tgaagtggga
    12361 ttagatcctg aaacacgcca ccgttatccg gcggagttct ctggtggtca gcgacaacgt
    12421 attgcgattg ccagggcatt aattcttaag ccctcgctga tcatacttga tgaaccgaca
    12481 tcatcactcg acaaaacggt acaggcgcaa atattgacgc ttttgaaatc attgcaacaa
    12541 aagcatcaac tggcctattt gtttatcagc cacgatttgc acgttgtccg cgcgttatgt
    12601 catcaggtta tcgtactgcg acaaggggaa gtagtggaac aaggaccgtg cgcgcgcgtg
    12661 tttgccacac cgcagcagga gtatacgcgt cagttactgg cgttgagctg acgcttaaaa
    12721 aggattgtag tttgaaaagg gttcggcaat tgccacacca aaatttttca atcggcatgt
    12781 tgcagcaaac tcgtcctggc gatttacaaa caggcacggc tcaccttcac actccaacac
    12841 cgaacatgga acttcgatgt cgcttaacgc ctggcttagt ttatacatca ccgaccatgc
    12901 attatcacca tccgggctaa gcaatttaag cgccaccagg ctgttgtcac cgcacggtcc
    12961 attttgagga atcggttcaa ccttcgaacc tgcgaaacca gcagaccagc gatagttctg
    13021 cggcagtcga tggacgattg agagttgtaa tgaagtcact tattttcccc ggaagtacat
    13081 tcacttcaca atttgtttac atcaatttta acacatcatc aacaatccgt ctttatacag
    13141 atcgtaaaga ttgatgcctc gcctgcgtgg cctctatggc tctgatttaa gtaatatctg
    13201 cgggcgtggt cacgtttgcg gggctatatg tacccgtttc tgcgatctaa ctcaaccttt
    13261 ttaactacaa tgatgtgact ttttacataa attgatttta cataaaataa acatatatcg
    13321 gggaaatgat agatttgtgg ttgacatgca tcaacaaagg aagcctttta ccttcctcgt
    13381 tgtgcaatag atcaccgttt tttcggccga ctggcgtaca gacagaagag aatggagctg
    13441 gttgcgcaga atgcaattga ccaaatcatc ggccatgcag agttaaaggt cgcaagagaa
    13501 agcaatgcgc caacaattgc ccctatgcca aaacggaagg ttcctgccag cgaagatgcc
    13561 gttcccgcca tatggggaaa ctcatcaaga atgaccgcca tcgcattgga tgacaccatc
    13621 gacacgcagc ccacaaacgc cgcaacgcca accaccagcg accagaatcc cagccccagc
    13681 ggcgcactga tgaccatcca cgcagccata ataaattgta tccacaaccc cgagcggaac
    13741 atatttaacg cgccaatgcg gcggacgaag cggctgttaa agatggtcat cacgaacaga
    13801 aaaacaatgt ttagcgcaaa gtaataacca aagttttccg gcgcgacgtg gttaatttca
    13861 atataaacaa acggtccggc gcttaagaat gagaacatcc cggcaaagct gaaaccactg
    13921 gcaagcatgt agctcaggac acgtttatgg cggaacagcg ccgcaaagtt accaatagtg
    13981 gtacgaatgt gaaatggctg acgacgctcc ggtggtaagg tttctttaat caggaagaaa
    14041 atcattgccg aagccagaat cgccgctaat gccaggatcc agaagatgta atgccagctc
    14101 agccacacca gcacccagcc gccaactatc ggtgccatca gcagtgcaat ggtcgtcacc
    14161 agcatgacaa acgacatcat ccgcgagaac tcttctttcg ggtaaatatc acgcatcaaa
    14221 gcgttgataa ccacgctggc cgcagccgca gccagcccgt ggaagaaacg catcacaatc
    14281 agctgatcga tggtttgtgc caacgcacac gccaccgcgg cggcggcaaa caccagcgtc
    14341 ccgccaagaa ccaccggctt acgcccgaag ctgtctgcca tcggcccgta gattaactgc
    14401 cccaacgcaa agcccagaat ataagtactg agggtcatct gcgtactgcc cgccgatacg
    14461 ccaaactgcg ctgaaattac cggtagcgcg ggcagataca tatcaatcga cagcggcatc
    14521 aacatggcca gcaggccaag gataaaaaca atagcaaacg acgaatgctg tcgggtggtc
    14581 acaacgggct cctgaaagtc attgaaaagt tagacgacgc tggcaatttc ttcttcagtt
    14641 aacggacgat attcaccggg ggctaaatca gcatccagcg taataccgcc aatacgttca
    14701 cgatgcagct caaccacgtg gttacccacg gcggcgaaca tgcgtttcac ctgatgataa
    14761 cgcccttcgc tgatggtcag acgaacctgc gttggggtaa tcacttcaag caccgccggc
    14821 ttagtgagat ctttttcgtt atgcagctgc acgcctttag caaattgctc tgccgtatcg
    14881 tcagctacag gtgattccag tgtcaccaga taggtcttct cgcaatgatg gcgcggagaa
    14941 gtaatgcggt gcgaccactg accatcatca gtcatcagca ccagaccggt ggtatcgata
    15001 tccagacgtc ctgcggcgtg cagtttccac gctaccggtt catccagaaa atagagcacc
    15061 gtcgggtgat ccgggtcgtc cgtggagcaa acatagccct gaggcttatt gagcatgaag
    15121 taacgtggac cgtgttgctg cgccagcggg ttgccatcgt aagcgacatc atgttcaggg
    15181 agcagtttga acgctgcatt acgaacgatt tcgccatcga cggtgacacg attgccgcgg
    15241 atttcacgcc cggcaatagc acggctaacg ccgagttgct gtgcgataaa tttatcaagt
    15301 cgcatgtgtg tgattttgcc tgtaaaaaac ggaggtcggg taatgacccg aaaatctgaa
    15361 ctgttgtctg cccagtatag cggtctaata acttccctca agggaaaaag aaccgtggca
    15421 tactattagc agaataatct acctacgcga aaccatgatt tttacactcc gcccatatca
    15481 gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt cataaaaccc ctgccgttat
    15541 cgtgctgccc accggcgcag gtaaaagcct ggtgatagcg gaactggcac ggctggcgcg
    15601 tggtcgcgta ctggtgctgg cacacgttaa agaactggtg gcgcaaaacc atgcaaagta
    15661 tcaggcgctg gggctggaag ccgatatttt tgccgccggg ctaaagcgca aagagagcca
    15721 cggtaaagtg gtatttggca gcgttcagtc ggtcgcccgt aatcttgatg cctttcaggg
    15781 tgaattttcg ctgttgattg tcgatgaatg ccaccgtatt ggtgacgatg aagagagcca
    15841 gtatcagcaa atcctcactc acctgactaa agtgaatccc cacttacgcc tgctggggct
    15901 gactgccacg ccttttcgat tgggcaaagg ctggatctac cagttccatt atcacggcat
    15961 ggtacgcggc gatgagaaag cccttttccg tgactgcatt tatgagctgc cgctgcgtta
    16021 tatgattaaa cacggctatc tgacgccgcc agaacgactg gatatgccag tagtgcaata
    16081 cgatttcagc cgcttgcagg cacagagtaa cgggctgttc agcgaagctg atctcaaccg
    16141 tgagctgaaa aaacagcaac gcattacgcc acatatcatc agccagatca tggagttcgc
    16201 cgaaaagcgc aaaggggtaa tgattttcgc cgccaccgtt gaacacgcaa aagagattgt
    16261 gggattactg cctgccgaag atgctgcact gattactggc gacaaccccg gcgctgagcg
    16321 cgatgtgtta attgaagatt ttaaagccca gcgttttcgc tatctggtca acgtcgcagt
    16381 actgaccacc ggatttgacg ccccgcacgt cgatcttatc gccattctgc gccctaccga
    16441 atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg cgtctcgctc cgggcaagac
    16501 tgattgttta attcttgatt atgcgggtaa tcctcacgat ctctacgcgc cggaagttgg
    16561 tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt ttctgccctg cctgcggttt
    16621 tgccaacacc ttttggggga aaacgaccgc cgacgggaca ttgattgaac actttggtcg
    16681 ccgctgtcag ggatggtttg aagatgacga cggtcatcgc gaacagtgtg acttccgttt
    16741 ccgttttaaa aattgcccgc aatgtaacgc agaaaatgat attgccgctc gccgctgccg
    16801 cgaatgtgac acggtactgg ttgatccgga tgatatgtta aaagcggcgc tacgactgaa
    16861 agatgcgctg gtattacgct gtagcggcat gtctttgcag catgggcacg acgagaaagg
    16921 cgaatggttg aaaatcacct attacgatga agacggcgcg gatgtgagtg agcgtttccg
    16981 tctgcaaacg cccgcccagc gtaccgcctt cgagcagctt tttatccgcc cgcatacgcg
    17041 cacaccgggc atccctctgc gctggatcac cgccgccgat atcctcgccc agcaagcctt
    17101 actgcgacat ccggattttg tcgtcgcccg catgaaaggt cagtactggc aagtgcgtga
    17161 aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac gaattacgcg gttaatggta
    17221 gttttgattg atgtataagg cgattgagta tagaatctcg cccgcttttg catacgcaaa
    17281 gcagatcact tacctgttgc tgggtcgcct gtagcaggat taatttaaga gagaaagaaa
    17341 tgtttactat caacgcagaa gtacgtaaag agcagggtaa gggtgcgagc cgccgcctgc
    17401 gtgccgctaa caagttcccg gcaatcatct acggtggcaa agaagcaccg ctggctatcg
    17461 agctggatca cgacaaagtc atgaacatgc aagctaaagc tgaattctac agcgaagttc
    17521 taaccatcgt tgttgacggt aaagaaatca aagttaaagc tcaggacgta cagcgtcacc
    17581 cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc ttaattgctg aataagttgt
    17641 aaaaaacccc gctccggcgg ggttttttgt atctgcagat tatgcctgat tacggtattg
    17701 ctattttttg caggccagat aaggcgttca cgccgcatct ggcattcaga acacaaaacc
    17761 gattaattgc cgccagacgt ccggcgctgc aattggtcgc gcaaattcgg cggcgtgcct
    17821 ttaatggtca aggtatcggt tgctggatcc cagaagatgc gttcacctaa cagcatcgca
    17881 tcaaagttaa tagtcaagcc accgccgcta ccggcaaact tcgtcaactg acgcagtgtg
    17941 ctacggtctg ccgggaagct ctcttccagt tcgtagcctt tttcagccgc aaactccgtg
    18001 aagctcactt cgcttacgcc tgccagctct ttcgacagcg attccagttc gatctcttcg
    18061 cctgcctgca gctgctcatt acagtagcta taaacctgct ggcgcacgtt ctgtcgctct
    18121 gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg cttgcaacag cccacggttt
    18181 tgcgctttcg cgttcaatcc ttcgctggcc ccgaggaaat ccataaagaa atccgccact
    18241 ttgcgcccta ctcgcccttt caggaaagtg agatagcggg tggactctgg attggtttcc
    18301 cattcggtta aatcgatacg cgcaacaata tccgcatgat tgatatcaag ataatgggtt
    18361 gggttgatgt cgagattttc gttaacgcgc atactgctca ggttgctcag aactgccacc
    18421 agcaaatact ccaccgccag ataacggtag tggcaaaaca gcacgaatcc accgtcagca
    18481 aaaggatatt tcgctaattc gtcacgcagg cgaccggttg ccgcgcggct aaatgccaga
    18541 aagtcctctt cgccctgacg ctgtaagcgc agcgtctgcg ccagttcact ctcttcgcta
    18601 aacaagccgt aagctttatt tttggcgcta tagacccgat gcagttctgc caccatctca
    18661 acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca ccagctcaag gttctgctca
    18721 tcacgcttga taagctggtg cagagcaatc tggttgatat ccagactcat gataaactct
    18781 cctttaagac cgggcggtat tcaaccaccg ccagttaagc gacgcaataa aagatggcgg
    18841 tcattttaca gaaggcagcg taacgagaat gatttaacgc ataaaaaagc agaaaaaaaa
    18901 ccgttgctac ggtaatatgt tgccctttca tcaactaact gatttcgatt tatgccacaa
    18961 atttcccgct acagtaatga acaggttgaa caattgctcg cagagctgct caacgtactg
    19021 gaaaaacata aggctccgac cgacctttcc ctgatggtgt taggcaacat ggtcactaac
    19081 cttatcaaca ccagcattgc cccggcccaa cgccaggcaa ttgccaactc ttttgccagc
    19141 gccttacagt cctctatcaa cgaagacaaa gcgcactaag ggaaacagat aacaggttat
    19201 ggtaactcat cgtcagcgct accgtgaaaa agtctcccag atggtcagtt gggggcactg
    19261 gtttgcactg ttcaatattc tgctttcgct cgtcattggc agccgttacc tgtttatcgc
    19321 cgactggccg acaacgcttg ctggtcgcat ttattcctac gtaagcatta tcggccattt
    19381 cagcttccta gtgttcgcca cctacttgct gatcctcttc ccgctgacct ttatcgtcgg
    19441 ctcccagagg ctgatgaggt ttttgtccgt cattctggcg acggcgggaa tgacgctatt
    19501 actgatcgat agcgaagtct ttactcgttt ccatctccat cttaatccca tcgtctggca
    19561 gttggttatc aacccagacg aaaatgagat ggcgcgcgac tggcagctga tgttcatcag
    19621 cgtgccggtt attttattgc ttgaactggt gtttgcgacg tggagctggc aaaagctgcg
    19681 cagcctgacg cgtcgtcgac gcttcgcgcg cccgctggcc gcattcttat ttatcgcctt
    19741 tatcgcctcg catgtggtgt atatctgggc cgatgccaac ttctatcgcc cgatcaccat
    19801 gcagcgcgct aacctgccgc tttcgtaccc gatgacggcg cgacgtttcc ttgagaagca
    19861 tggcctgctt gatgcgcagg agtatcaacg ccgtcttatt gagcaaggta atccagacgc
    19921 cgtttccgta cagtatccgt taagcgaact gcgctatcgc gatatgggca ccgggcagaa
    19981 tgtgctgttg attactgtcg atggcctgaa ctactcacgc ttcgagaagc agatgcctgc
    20041 gctggcaggt tttgctgagc aaaatatttc gttcacgcgc catatgagct ccggcaacac
    20101 tacagacaac ggcatctttg gcctgttcta tggcatctcg ccgagctata tggacggcat
    20161 tctgtcgacc cgtacgcctg cggcattaat tactgcgctt aatcagcaag gctatcagct
    20221 ggggttattc tcatcagatg gctttaccag cccgctgtat cgccaggcat tgttgtcaga
    20281 tttctcgatg ccgagcgtac gcacccaatc tgacgatcag accgccacgc agtggatcaa
    20341 ctggctgggc cgctacgcac aagaagataa ccgctggttc tcgtgggttt ctttcaatgg
    20401 cactaacatt gacgacagca atcagcaggc atttgcacgg aaatatagcc gggcggcagg
    20461 caatgtcgat gaccagatca aacgcgtgct caatgcactg cgtgattctg gcaaactgga
    20521 caatacggtg gtgattatca ctgccggtcg gggtattcca ctgagcgaag aggaagaaac
    20581 ctttgactgg tcccacggtc atctgcaggt gccattagtg attcactggc caggcacgcc
    20641 ggcgcagcgt attaatgctc tgactgatca taccgatctg atgacgacgc tgatgcaacg
    20701 cctgctgcat gtcagcacac ctgccagcga atattcgcaa ggtcaggatt tgttcaaccc
    20761 tcaacgccgt cattactggg ttactgctgc ggataacgat acgctggcaa ttaccacccc
    20821 gaaaaagacg ctggtgctga acaataacgg taaataccgc acttacaact tacgtggtga
    20881 aagagtgaaa gatgaaaaac cacagttaag tttgttattg caagtgctga cagacgagaa
    20941 gcgttttatc gctaactgat taattataaa tcagttagcg aaatatctta cttgcaatcg
    21001 gtgtggaaaa cggtagtatt agcagccacg agtcggcacg tagcgcagcc tggtagcgca
    21061 ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc gtgccgacca aaaatcccaa
    21121 gaaaaaacca acccttacgg ttggtttttt tatatctgca attaattcga taaacagacc
    21181 gtgacacatc acagcctgtt tattttctgt tatcagaacg tccagaccac acccgcctga
    21241 gtattccacg gcgattctac ccctgcgcca ttgccatagc tgactgacaa atgaccgctt
    21301 aacgtcgggg taaacgatga ccttatccca gcctgataaa cgccactggt tccagccaca
    21361 tcattattaa atttgccatc gtcattaact ttcacctggt tagaatcggc gtattcctga
    21421 cgcacggcgg ctttcagcca gggttccagc gtcgtaccgt tttgcaggtc catgtgatag
    21481 cttaccgccg ttcccgcttc agcgcgtaat atccgggtat ttcccacatc cgcgcgcatg
    21541 ccgtttgata acgtgtagtc ctgaccatct gtggtaaagc cggtaaaggc cagatagggt
    21601 ctaacactcc acaatccgtc aacccaacgg aacccgctct caacatgagc acccgcgccg
    21661 ttactattgt aatcgccaaa cgctgttgcc ccattactca tcttgccatg gatggtgttg
    21721 gcaaaacggt caactttcac caccccatca acataggcac cgttctgatg ctcccaaccg
    21781 gcataagccc ccagggtata gctatcgata ttaccttttc cgccgcgatc aaaaccaata
    21841 tcagaatgag agtaaccaaa gaacaagccg cgaattgtac tgctttcttc acgggagaaa
    21901 cggctatcga taccgagcgt caggcccgtc aatgtttgct caaaaccagc tcccgcatca
    21961 gtggtcacgt tgttgcgggt gttaattgcc gaactccaca tcaccgtatc gtaactaacg
    22021 ccttttacgc taccaagacg ctcacgcacg gtgtccagtt ctgcatcaaa taccagcggt
    22081 tgtgcggctg ccatgttcag cacatcagtg gttgaagggg taatttgcgc gcgattctct
    22141 gcgaggctcc agctatggtt gccattatcc agcaaggtat attcatacgt accgatatca
    22201 acaacgcctc cggcattgcc caacgtaaat gcagcatcac cgccgcccgt tgttaccagt
    22261 gtaaggctat ctcctgctgc cgggctggca ccggtgtccg tcacgaatat tttgaaatca
    22321 cctgttgcct gaccggtgac gttgagctga tcgctctgat gattggccat atcggtacgc
    22381 atataaaaat tgccgtttcc cgacagggta ttggtggtca acgtaatgaa attttccgca
    22441 cttgccgtca cagatgaacg agtcactggc tcagccatca taatcacatt tgcgccggac
    22501 atcgcaaggt tggctaagtt gaggcgagtg ttttggttcg tcgtagtgat atcggcaatg
    22561 atggtccatg cgctattttc gagcgaaacg tccgctttgc tggtatccac ggcaccatgc
    22621 gttctaaaag aagcgccctc agaaaccact accttaccca ccaacatagc gggtgcataa
    22681 tttatttgcg gcttcatgac ctcaagaatg ccatcattcc ctctgactga cacgttaacc
    22741 attgtgccag cccagacatt agcgcggcca ttgttgatta ccatattttc agagatggct
    22801 tttgaggaat acgtgatact tccattctgg tctgatcgac cgagatcata taccgcattt
    22861 gcctgcactt tcgtaccagt atcctttccc agcgattgca tagtggcatg ctcatcaagg
    22921 atagtgtcaa tggcctgcgt gtcctccata acaatgagcc cggaaccatc atccagttca
    22981 taattttttg acacaccatc ctttatacta aattggcctt tactgtttgt accactcact
    23041 tccagcgcat tcgttgagac aattaatttc ccgcctgctt ttttatcaac gccagttgca
    23101 gtcccgccat ccataacctc cagtaagccg ccactatcca cagtagtatt ataagcgaag
    23161 tcattttctt caactcgcaa acttccgccg ttttccaaca acatattatt agcaatacca
    23221 ttcttgatat cgaactgacc gagacggttt gttccgaata cctccatggc ccgcgtggtt
    23281 gttttaatag caccgcctgt tttctgatct accgcaagcg caaatccccc ctctttaacc
    23341 tccagtagtc ctcccttttc gatagcggca aaaacggccc ccccatcttt ttcaacgatt
    23401 aagcgcccgc ctttaatcgt ggtatcattc gctgggccat aaacctgtag cacaccatta
    23461 ttgagggtag tctggtttgc cgcgccaccc atttgaacaa tttgataacc atcatttaat
    23521 actgagtatt ctgcggtacc attcatttca atgtattgat accctccgtt aatttcagta
    23581 ttactactta caccaaattc ttttatatgc tgttctccgc caggattgat tttcgtatta
    23641 ttggcggtcc cataaaccag ctggatatct ttttccagga taacaccatc aactgtctca
    23701 ccatactcaa ctgttgatgc caatgctatg ggtgaatata acgctgtagc caccagaaca
    23761 gatattgcgg aacgacaaag agaaacagaa ccagattgat gcattgagct ttcatcctat
    23821 gaaattaatt gcagttaaaa gcattgggta cagaaaatac ccatagctcc atacccggag
    23881 tcagttttta aaaactgttt aaagaaatgc acaagtattg tgattgattt tttagttgtt
    23941 tttcttgatg agaagctgat gcaaaattcc gtctttataa taaaaatgat gccaaagcga
    24001 acgacaaggt tgtagttttc actacatgtc catacataaa atggggtaac attcacgcgc
    24061 ctggtagcgt taccaacgct acgctcaaac ataatgattc taataaaacc tcaggagact
    24121 actatgcctg aagcaacgcc ttttcaggtg atgattgtgg atgatcatcc acttatgcga
    24181 cgcggtgttc gtcagttact ggagcttgat cctggctttg aagtggtcgc cgaagcgggc
    24241 gaaggcgcga gcgcgatcga tctggcgaat agactggata tcgacgtgat cttgctggat
    24301 ctcaatatga aaggtatgag tggcctggat accctcaatg ccttgcgcag ggatggcgtt
    24361 accgcgcaaa ttattatcct gaccgtatcc gatgcctcca gcgatgtctt tgcgctgata
    24421 gacgcaggcg cagacggtta tctgttgaaa gacagcgacc cggaagtatt gctggaagcg
    24481 attcgtaccg gagcgaaagg cagcaaagtc tttagcgaac gcgtcaatca gtacttacgt
    24541 gaacgtgaaa tgtttggcgc ggaagaagat cccttcagcg tgctgacgga gcgcgagctg
    24601 gatgttctgc acgagctggc acaggggctg tcaaataaac agattgcatc ggtgttgaat
    24661 atttccgagc agacagtaaa agtacatatt cgcaatctgc tgcgtaaact caatgtccgc
    24721 tcacgcgtgg cggccaccat tctgttcctg caacaacgcg gggcacaata aaaatagccc
    24781 gatggattta tcatcgggct gagatttatg acaaacgcaa aactgcctga tgcgctacgc
    24841 ttatcaggcc tacgtggatc gatcaattta ttgaatttac acaattttgt aggccggata
    24901 aggcgttcac gccgcatccg gcataaacta agcgcacttt gtcatcagtc tagcccgatg
    24961 gcatcaccat cgggcctctt tttatttact ctcctgcggc gacaaatgtt gcatcgcctg
    25021 cgcgatacta cgttcaatca ccgcacggcg agtatcgttg gcaggtaaga gtttcaacat
    25081 catctcccac gcggcaacgg cttcgccaaa tcgctgctgc tcaaaggcat taaacgcata
    25141 catgcttagc acacggatat tgctatggtc cgttctcacc agctgacgta gcagttcacc
    25201 gccgaggcgg ttgtcgttgg gatcagatga acgagtcaac gcttcagcgt atcccagtgc
    25261 ggcatcgctg ttcttcggat cgaggcgata cgcagtagcg tatgcatcgg tggcgatact
    25321 ggcgttaccc agcgccatgc caacgcggcc caacataatc cagccttcta tatctcccgg
    25381 atttttttgc agttgagtac gcatccccag cgcaaggcgc gacatctctt cttcgttgag
    25441 cggatcggct ttcgggtcca gcgccctgtc cagcaacgcc ggagcctgtg ccgtggcctg
    25501 ctgccagatt ttcacctgct gataattgcc agtctggtag tagctgacgc cagccacaat
    25561 taacgccacc acaatacccg gcagataaac aacatatccg gcacgcttac cttccggcac
    25621 gctttgttca ggaaacgctt ccggcaccac gcgtacccga cgccgcgagc gggcgtatat
    25681 gacccaaccg ccaatgccaa tagccactac tggcagcacc cacagcagca cggtcagcgg
    25741 cgttaacggc ggatcgtaag tgacgaagtt gccgtaacgc gccaccatat aatcgacaat
    25801 ctctttttta cttttacctt cctgcatcag ttcatacact ttctgacgca ggtcggtggc
    25861 aatcatcgag ttggaatcgg caatgctgtt gttctggcat ttcgggcagc gcagttcttc
    25921 agtgagctga cggaactgtt gttcctgtgc ttcatcttta aactgcaaca cgtcgatggt
    25981 cgccagcgct gagccggaga tcatcagcat cagcacgccc aataaaaacc tcattgtgcg
    26041 gcctccttac tgtatttctc ccacagcggc ttgatctctt cttcccagac gcgaggattg
    26101 agatcgcccg catggcgata gcgaatgata ccgttgccgt caataaggaa ggtttctggc
    26161 gcgccataga caccgagatc cagccctaac atgccgtcgc catcaaacag gcttagcgcg
    26221 taaggattgc ccagctcttt cagccagctg attgccttct ggcgatcgtc tttatagttc
    26281 atgccgacca cgcgaatgcc ctgcgctgaa agctgattca gatattgatg ttccgcacgg
    26341 caggtcggac accaagtcgc ccagacgtta agcagtactg gtttgccctg agtcagcaca
    26401 tccgcctgat aaaactgccc cggattgtcc agtgattcga gacgaaactt cggcacaggc
    26461 ttgccaatga gcgccgattc cagattggtc ggatcatccc cttcggcatt acgcgccagc
    26521 tgccacagca gcgccgcggc aatcgccagg aagataatca acggaattaa caatactttg
    26581 cgcttcatac ggcctccggc gcagtttttt gcggactcac gcgcttacga tagcgaggat
    26641 caaacagaca cagcagtccg cccaacgcca tcatcagccc gcccgcccaa atccagcgaa
    26701 caaatggttt gtagtaaaga cgcacggccc acgcgccgtt ttccagctct tcaccgagcg
    26761 cggcgtacag gtcacgcgtg atgccgccgt caattgccgc ttcggtcatc atcgacccgg
    26821 cagtgttgta ataacgtttt tccgcataca gcaccgtttc cggtttaccc tctcgcgtta
    26881 cgccaatagt cgccacaccg ccgcgccagt tcgggccagt cacctctttg acatcacgga
    26941 aggtgaagcg atattcatga atatcgacgc tatcgccgga cttcatgcgc acatcacgct
    27001 caacgctata attctggcta aaggcaatgc caacaattgt cactgccagc cccaggtgag
    27061 ccgccaccat cccccaataa ctgaaggtgg ttttcgtgcc gcgtgaaata cgcagcgcag
    27121 cttccgcagt tgccagcacc gcaatccagc aggccattgc caggccgagc accgtcatcg
    27181 ccacaacttt gctttcgaac agccacggca acagcagaga cagcaccagc gtagagatga
    27241 aggcgataat caataaattg cggattttac gtgggcgatc gcgcccccag cgcaccagag
    27301 gaccgacacc aagcagtagc gcaaacggca ccatcagcca ggtaaacatg gtgttgaaga
    27361 acggttcgcc aatcgaaata ctgcccagtc caagttgctt gtgcaccagc ggcagcagcg
    27421 tccccagcaa caccaccagc atcgcggcga ccagcaaaac gttgttcgct aacagcaaag
    27481 attcccgcga ccacagcgca ttgtttacgc gtgagcgaac tttgtgtcca cgcgcggcaa
    27541 acagcagcag cgaaccgcca atcaccagca ccataaaggc gaggataaac ataccgcgcg
    27601 ccggatcaga cgcaaacgcg tgtaccgata ccagcacgcc ggaacgcacg aggaaagtcc
    27661 ccaacagaca caacgagaag gcactgattg ccagcagtaa tgtccacgct ttgaagctgg
    27721 cgcgttgttc agtgaccgcc agtgagtgca tcagtgcagt ccccaccagc cacggcataa
    27781 acgaggcgtt ttctaccgga tcccagaacc accagccacc ccagccgagt tcgtaatagg
    27841 cccatgcgga accgagcaca atgccgagcg ttaggaaaat ccacgccgcc agcgtccacg
    27901 ggcgagtaaa acgcgcataa gtgctgtcca gacgcccgct cagcaaagaa gcaatggcaa
    27961 aagcaaacgc caccgagaaa cccacgtacc ccatataaag cagaggcgga tggaagatca
    28021 gccccggatc ctgtaacagc gggttaagat cgcgcccttc aatcgggaag ttcggcagcg
    28081 tgcgtgagaa cgggttagag gtaaagagaa tgaacagcaa aaagccgacg ctgaccatcc
    28141 ccattatcgc cagtacacgg gccacaatat ccagcggaat acgctgacta aaaatcgcca
    28201 ccgcaaaggt ccagccgctc atcagcagca cccacagcag aagcgaacct tcatgcgcgc
    28261 cccaagtagc cgccacgcga taccacaccg gaagctgggt attggagttg ctggcaacat
    28321 aggtgacggt aaagtcgttg accacgaaag cattgaccag caccagaaat gcgccagcca
    28381 cagacataaa cagcagccag gcaaacaagc gggaagacgc catcatgcgc gcatctccgc
    28441 gcgccacgcc ccatagcgga tacacggaca gcagcagcgc aatccctaac gccaagcaca
    28501 gcagcccgtt tccaatttcc ggcatcatga tgctgggtcc ttataaacac tcgccgggcg
    28561 acggtggtta gcttccatcg ctttctcaac ttctggcggc gtgtagtttt cgtcgtgttt
    28621 cgccagcact tctttcgcga ggatatgatt gcctttttcc agctcgccct gcaccacaac
    28681 gccctgccct tcacggaaca gatccggcaa aatgccttcg taagagacat ccactgagcc
    28741 ttcagcatcg taaatggtga aggtcacttt cagcgaattg ggatcgcgct gcacgctacc
    28801 cggcatcacc atcccgccaa cgcgcagacg ctgaccgact tccggcatct gctgagtttc
    28861 acgcttgccg tagagaattt cccccggcgt ataaaagaga tcgatattcg agcgcagcgc
    28921 atatagcacc agaccgatag tcagcgctag ccctgccaac acggcacagg caatccacaa
    28981 gcggttttta cggcgaatat tcatgcagcc tcctgctgtt gcgcagcacg taaacgcgcc
    29041 tcacgcgccc gctgttgcgc cacaccacgc agaattgcgc gatgttgcat caccgagtgc
    29101 acgaccaaaa ccaccagcgg aataacggtc atcaccaccg ccagccagac aaaaaaggcg
    29161 taaccgccca ttgcgaaaaa ttcattccag gaagcaaatg caggggtcat ttacggcctc
    29221 ttttcagtat cagttcactc acccacggac ggcgtttttc catcagcaaa atcaaattac
    29281 gcatccgcat cagcgtcagc gtggcagaca ggagcaggaa gccaaaaatc gaccagcgca
    29341 gcggcgaacg catcgccgga tcgatacttt gctgcatccg cgttgatccc tgatgcaggg
    29401 tgttccacca ctccacggag taatgaataa tcggcagatt caccacgcca atcagcacca
    29461 tgatacctgc cgcacggccc gccagacggc ggtcgtcgaa ggcgtgccac agggcaatca
    29521 cacccacata caaaaacagc agcaccagtt cagaagtcag acgtgcatcc catacccacc
    29581 aggtgcccca catcggtttt ccccatgcag agccggtaac cagggcaata aaggtaaaca
    29641 cggcaccaat gggggccatc gccgccaccg ccaggttggc cattttcatc tgccagacaa
    29701 ggccaataaa cgccgccacc gccattgatg cataaatgcc catcgaccag atcgccgcag
    29761 gcacatgcag gtagataatg cggtagctat ttccctgctg ataatcagcc ggagcaaagc
    29821 cgaatcccca gatccagccg acggtaagca cgaccacact ggcaattgcc agccacggta
    29881 taaaccagcc acagatttga tacagccgtg gtgggatcgc cagttgatgc agtgttttcc
    29941 acatagtttc gataccagac tcgaacaaaa atcagtaatc cagcgttatt gaatgctgat
    30001 tcgtaacgct gccgccgtcg caaaaggact taatgtcgcg gtgcctgcca gcaacgcgcc
    30061 taaaattgcc agatacccat caacgggcaa atgcatagaa gccgcgtcca tcgcggcggt
    30121 ggcaaagatg agtaatggga tagtcagcgg taacaccagt atgctgagca gcacaccacc
    30181 gcgcttaagt cccactgtca gcgccacgcc cggtgcaccg agaaagccaa gcgtaggcgt
    30241 tcccagcagc agcgtcagcg ccatcacttg ccagccataa acatccattc ccagtagcat
    30301 tgctaccagt ggcgaaagga tgagtaacgg cagaccggtt accatccagt gcgccatcac
    30361 cttcgccagc acaacggcgg gcaagggtaa cggcaacaac atcaattgtt caagactgcc
    30421 gtcctgcaaa tcgtcacgga acagtcgttc cagcgccagc aaggatgaaa gcagcgcagc
    30481 aacccagata atgcccggtg caatacgcgc cagcagttgc ggctccggac cgatactgag
    30541 cggaaaaagg gtaattacaa tcaggaagaa ccacagcggg ttggcgattt cggcgctatg
    30601 gcgaaacgct acgcgcagtt caagacggaa aatacgccag aacatcatgc ggccctcgtt
    30661 tgcgtcagtg aaatgcggcg aattttactt tcagcaacgt tgagcggctg gtgggtagtc
    30721 agaatcacaa tccccccctg ctccgtatgc tgcgccatac gctgggtcag acgatcgacg
    30781 ccgttaacgt caatcgcggt aaaaggctcg tcgaggatcc ataatgtggc acgggtcaac
    30841 cacagacgcg ctaaagcgac gcggcgttgt tgcccggcag aaagttgatt taccggaata
    30901 tcttcgaatc cggcaagccc ggcctgcgcc aaggcttcca gacattgtgc ggcatcgcca
    30961 tcgcgatgat aaaagtgcag attttctaac gccgtcagcc gggttttgat ccccggctga
    31021 tggcctatcc ataacagggt ttgatggtag ctgtcgcgta cctgatgcaa gggctgccct
    31081 tgccagagaa cctcgccagc gtcagggcga gacaaccccg tcagcaaacg gagaagcgtt
    31141 gtcttccccg cgccgttgct accggtgatt tgtacccact ctcctgcgtt cagcgtaaat
    31201 gacaagccac taaataaggt tcgttcatcc cgctcacaaa gtaactctct ggcttcaagc
    31261 atacccacgc aataaccctg ttaaaaacct ggctcgactt cacgcatatc gggcagcttg
    31321 tgcgcgatcc ctttatggca atcaatacag gtttgcccat ctttcaccgc ctggtcatgc
    31381 atcttcgcgg caaccgattt ctgggcggtt gtatccatat actcgaagtt gtgacagtta
    31441 cggcactcct gcgagttatt gtccttcatg cgccgccact cattctgtgc catcgtcaga
    31501 cgatgagctt caaatttctg cggcgtgtca ataacgccaa aaattttacc atacagctct
    31561 ttacttgctt tgagcttgcg tatcatcttc ggcacaaact cgtgcggaac gtgacaatcc
    31621 ggacaggtcg cacggacgcc gctacggttg ttgtagtgca cggaatccat gtattcctga
    31681 tacaccgtgt tgcgcatttc gtggcagcta atgcagaact cttcggtatt ggctttttcc
    31741 atcccggtgt taaagccccc ccagaagacg atgccgccaa caaaaccgat caacagcagc
    31801 gtccccagcg ccagatggct gggggtacgc caccatttcc acaggcgctt aatcagacca
    31861 ggcttacggt cagaatttcc cataataacc tcttatttcc cgtaaccttt tgatggggta
    31921 aaggtattcc ccacgattgg cgcggtatcg gcctgcggta cgtgacattg cagacagaaa
    31981 taacgacgcg gagccacttc cgcgcccact ttgccgtcgc tgtccataaa gtgagtagga
    32041 ctgatacgcg gcgcgccagt ggtgcgatag ctttcgacac cgtggcactg caagcagcga
    32101 ttggtattgg tcgttacctg ataaccttca acgctatgcg ggatcattgg cggctgattc
    32161 acatagttca gcggcatccg atcctgctct tttggcatcc gaatggcccc ttcctgcgtc
    32221 ccggatactt ccggcgactg gctaaaatcg acgccattag ccgcccaaac cgcgccgctt
    32281 accaccaggg ccagcatcgc cgtccattga cacagcgctt tcttcaggtc atggcttttc
    32341 atgatttcgc tcccgaactc catcgtgtag ttattgtaaa tacatcctca gaacagacat
    32401 ccacgcagcg accgcacgtc atgcaatcgc ggctggtgac ctgcaccggg ctttgttcat
    32461 ccagcaccgg ggcacgtagc acatgcggtt ccgggcaaac atgaaaacaa tccatacagc
    32521 ggttacattt ctgccgatcg ctggccgcaa cggtaatcac acctttgcta cccagcacgc
    32581 catacagcgc gccaaccgga caaatgtgcc cgcaccagcc gtgttcaacg accagtagat
    32641 caaataaaaa cagcgcgaga ataagcagcg cgccgctgcc gaagcccatt accaggctac
    32701 gccccatcag agaaacgggg ttgatccatt cccaaatgag cgtgccagtt aaggctgatc
    32761 ccaccagaat gaccaccaac agcacgtagc gaatgtggcg agggatcgtc gcagactgat
    32821 tgaggtcaaa gcgcctgcgt aaccagttcg ctaagtcggt aatcgggttc agcgggcaga
    32881 cccagctgca aaataatcgc tttcccgcca gggcgtagag cacggtgata atcaccgcac
    32941 cggtcaacgc caccgtagca ggcagatgac cactggcaag actttgcagc gtcatcagcg
    33001 gatcggtcag cggaacggtg tcgaataaca ggctactgct gtagttgccg tgcaagatcc
    33061 acacaccaaa ccatggaccg ctcaaaaaca tccccagcac gaagaactga caaaggcgac
    33121 gtaacaccag ccaacggtga ctgcgccacc agcctttttt ctccagcgcc tcgcgcccgg
    33181 cgtcacgttt acgatttgcc attgttcccc tccagccagc cgaagcggta atggtgacct
    33241 aactcccctt tcgccagtga cagcggtaac accttgattg ccggttgttc cagcacgcat
    33301 actttttcgc acttaccgca accggtacag gcgtcgctgt gaaccgtcgg cagaaagcgg
    33361 gcgtgcttac cggtacgcgt gttgcgctcc agctccaagg tgatggcctc atcaattttc
    33421 gggcattcgc gataacaaac atcgcagcgc agcccctgaa agttgagaca gttttcctgg
    33481 tccaccagta ccgccagccc catccgcgcg tcgtcgatcg attcaatctc acgatccagc
    33541 gcaccgcttg ggcacacttt ggcgcacgga atgtcctcac acatttcgca aggaatatcc
    33601 cgtgcgacaa aatatggcgt gcccgccgac agaccagagg ccagcgtcgc cagttttaag
    33661 gtgtcgtaag ggcaagcctg aacacactga ccgcaacgca cacaggcact ggcaaaggcg
    33721 ttctcgttta tggctccggg cgggcgcaac cgcacgccag atgcgcgtgc ggtttgctgt
    33781 tgtaacccca gcgccacacc cacggcagcc agcccgcctg ctgtgcgaac gacatcgcgc
    33841 agaaagcggc ggcgaccatt ttgaggtttc gctgaccggg acataatggc ttacaccttc
    33901 tccagtttga ccgcgcactt cttgaagtcc gtctctttcg agagcggatc ggtcgcatcc
    33961 agcgtcagtt tgttaaccag ctgtgcggcg tcgaagaacg gcatgtacac caggccctgt
    34021 ggcggacggt tacgaccgcg cgtttcaaca atcgagatca cttcgccacg gcgagaaacc
    34081 actttcactt tgtcgccacg gcgcagatcg cgcgctttcg catccagcgg gtgaataaac
    34141 aagaccgctt ccgggaaggc gcggtgcagt tccggcacac ggcgagtcat actgccggtg
    34201 tgccagtgct ccagaacgcg tccggtagag agccacaggt cgtactcttc atccggtgct
    34261 tccgccgccg gttcgaacgg cagtgcgaag atcaccgctt tgccatccgg tttaccgtag
    34321 aacttatagc cttcgcccgc tttcacgtac gggtcgttac cttcgctgta acgccactgt
    34381 gtttctttac cattcaccac cggccagcgc agaccgcgcg ctttgtggta gtcatcgaac
    34441 ggtgccagat cgtgaccgtg accgcgacca aaccatgcgt actcttcgaa cagccctttt
    34501 tgcagataga agcccagctc gcgggattca tcgttcagct gatcttccgc cagttcggat
    34561 accgggaatt tgctcacttc gggggtggca tacagaactt cgtacagcgt tttgccacgc
    34621 agttccggtt tcttcgccag cagctcttcc ggccatactt cttcagtttt gaagcggcgg
    34681 gagaactgga ctaactgcca gagatccgat ttcgcttcgc ctggcgcctg tacctgctga
    34741 cgccagaact gagtacggcg ttcggcgtta ccgtaagcgc cctctttctc tacccacatt
    34801 gcggtcggca ggatcaagtc ggcggccagc gcactgactg tcggatacgg atcggagacg
    34861 atgatgaagt tgcgcggatc gcgccagccc ggcatacgct cttcattgat gttcggcccg
    34921 gcctgcatgt tgttggtaca catggtccag taaacattca gcttgccgtc tttcagcgca
    34981 cggtcttgtg ccaccgcatg aagaccgatt ttcgccggaa tggtgccgct cgggatattc
    35041 cacttcttct cacagatatc acgatgtttc tcgttagtta ccaccatgtc cgcaggcaga
    35101 cggtgagcaa aggtgcccac ttcacgcgca gtaccacacg cagaaggttg cccggtcagg
    35161 gagaacggac cgcaacccgg ctgggaaatt ttgccggtca gcaggtgcag gttgtagacc
    35221 aggttgttag cccacacacc acgggtatgc tggttgaagc ccatcgtcca gtaggagatg
    35281 actttcttgt tcggatcggc atacagctgc gccagttgtt ctaactggtc tttcggcacg
    35341 ccggtcattt cagcggtttt ttccagcgta tactcagcaa cgaaggcttt gtaatcttca
    35401 aagctcatcg gttcggaggc gtcagaaccc ggattcttcg ctgctttttc cagcggatgg
    35461 gtcggacgta aaccgtagcc gatgtccgtc gcccctttgc gcaggttaac gtgcttgctg
    35521 aagaagtcct gatttatcgc attgttttga atgatatagt tggcgatgta gttcaggatc
    35581 accaggtcag attgcggcgt aaagatgatg ccgttatccg ccagctcgaa gctacgatgc
    35641 tggtaggtag aaagcaccgc cacggtgacg ttctggttag agagacgact gttagtgatg
    35701 cgtgaccaga ggatcgggtg catctccgcc atgttagcgc cccacagcac aaacgcgtca
    35761 gcctgctcga tgtcgtcata gcagcccatc ggctcatcca taccaaaggt acgcataaag
    35821 ccaactactg ccgacgccat acagtgacgt gcgttcgggt cgatgttgtt cgaacggaag
    35881 cccgctttga acagcttgga cgcggcataa ccttcccaga tagtccactg accagaaccg
    35941 aacataccga tcgattccgg ccctttttct ttcagggcgg ttttgaattt ctcttccatc
    36001 acatcgaagg cctgatccca ggtgattggg gtaaattcgc cttctttgtc atatttaccg
    36061 tttttcatac gcagcagcgg ctgcgtcaaa cggtctttac cgtacatgat cttgggcagg
    36121 aaatagccct taatgcagtt caggccacgg ttaaccggtg cgtccgggtc gccctgacag
    36181 gccaccacac gcccctgctg cgttccgacc agaacgccgc aaccagtacc gcagaaacgg
    36241 cacggcgctt tatcccattt aatggcttcc tgctgaccaa caacggcgcg ggcaacgccc
    36301 ggcacgctga gaccggcagc cgccgcagcg gccgcaacgg cgttagcttt cataaagcta
    36361 cgacgactga gtttcatggt gtttcctcac cttgctcttc ctgctggtga taaaccagcg
    36421 acaccgccag cacgccctct acgttgcgta ctgactcaat ggtttggatc agcgtttcgc
    36481 tgtcttctgc ttccaccacc acaatcaact gaccgctcgg cgcgtcgctg acagcaactt
    36541 cacagccggg aaaggcgttc agttgggtgc tgatgtctga aattcgttcg cttttggcct
    36601 gcacgaccag gctgcaaact tgccagttag tgtgcatgga gatactccgc agttatggct
    36661 gataccggac agctggctgc acatgctcca catccgttgc aaagttggct attaagttgc
    36721 gcctggtaga tcccggagag cgtcgggcga aagataattg ccattggctc acagctatcc
    36781 tgacagcggc ggcattcaac tgactgatac gccaggcacg cgtccccgat ggtgaactgc
    36841 aaatcccagg ccctggtgtg gcgcggagaa aatagcgatt cggggcaagc ctgcgcgcag
    36901 gcgtaacaga agctgcactc gttatttttg aaattaacgc tcggatagcc acccgtgccg
    36961 cgttgcagaa tgttgttttc acaagcatta atacaggcgt cgcagcgggt gcaatgggtc
    37021 agaaaatgag attcatcacc ggaccagggc ggacggatac cgttactggc tttgcgccag
    37081 caaccagtga gtatgccccg acgggatgca tcaatcttca cattgacctt ccatcattaa
    37141 cgcgctctga aaattgagag cgaccaaata gaccgcataa ttaataagcc atttttatag
    37201 ccgctaagat attaaaggat gtgtcaaaga tgcatacccc gatcggggta aaacctgtag
    37261 caggatcaaa agagtggcgg gaagcgtggc aaaaacgggc ttttgctcac atttcaaatg
    37321 gttataaaca tatttatata gcgattaatt caccagagat atttctgctg gtttgctccc
    37381 tcatcagaat ttaacactaa aagagcaggt aaaattgtct gaatgttctt taagttattc
    37441 ataaagcaaa ttaataaatc tgatgaatat gttaaccttc agcgacatca tcggtgaaaa
    37501 cctataaatg aagaaggaaa gcaaaaaaat gaagaccatt ctacctgaag tattgtttgc
    37561 cgctttcgct accacttccg cctgggcggc agaaagcgtc cagccactgg aaaaaatcgc
    37621 gccttatcca caagctgaaa caggcatgaa gcgtcaggtg attcagttaa ccccgcaaga
    37681 agatgaatct accctgaaag tagaactgtt aatcggtcag acgctggaag tcgattgcaa
    37741 tttgcatcgt ctcggcggga agctggaaag caaaacgctg gaaggctggg gctatgatta
    37801 ttatgtcttt gataaagtta gctcgccggt ttccacgatg atggcctgcc cggatggcaa
    37861 gaaagagaag aaatttgtca ccgcgtatct gggcgatgct ggaatgctgc gttacaacag
    37921 caagctgccg atcgtggtgt atacgccaga caatgtagat gtgaaatacc gcgtctggaa
    37981 ggcggaagag aaaattgaca acgcggaagt tcgctaaact gttgtgaagt acggcacccc
    38041 gttggttgga taaggcggtt acgccgcatc caacatctaa cgcccgagcc ggttgcctga
    38101 tgcgacgctg gcgcatctta tcagtatacg ccacatcatt acaacgcaat atccgccact
    38161 tctttttgaa cgggttgcgg tttcaactgc ggtttcggcg tactatctgc tgcttgcggt
    38221 ttgtcgtatt tcagccccag cacttcgctg gtgtactgca actcgcgttc tgttgccgct
    38281 acatcaccgt tcagcttgcg tccataagac ggaacgatcg ctttcaacgt agcctgccat
    38341 tgcgggctgg aaacgcgatc gccaaatact ttttccagta gattcaacat aatcggcgcg
    38401 gcggttgacg cccctggcga tgcccccagc agcgccgcaa tggtcccttg ctggtcactg
    38461 accacttcgg tacccagacg cagtacgcca cctttctcgg catcacgctt gataatctgc
    38521 acacgctgcc ccgcttgcca caaacgccag tcctcttttt tcgcttgcgg atagtactct
    38581 ttcaacgctt caaaacgatc ctcttcactc aacatcacct gactcaccag atatttcacc
    38641 agatcgaaat tatccagccc gacgtgcatc atcggcatca cgttagaggt ggtggtggaa
    38701 ctcatcagat cccacaatga accgtttttg aggaatttgg ttgagaaggt ggcaaatggc
    38761 ccaaacagca ctacgcgttt accgtccaga acacgggtat cgatatgcgg aaccgacatc
    38821 ggtggtgcgc caacggatgc tttaccgtaa actttcgcca gatggcgatt aaccacgtct
    38881 ggattttccg aaacaaggaa ctgtccgccc accgggaaac cggcgtagtc tttcgcttcc
    38941 ggaatccccg attcctgtaa cagcttcagc gccgcaccgc ccgcgccgat aaagacaaat
    39001 ttggcgcgga tgttctgtgc agtgccgttt ttcagatcgg caacggtaac ggtccaggtg
    39061 ttatcgtcat tacgctttag ggcgcggact tcgctgctga gttgcagcga gaagttagat
    39121 ttcttctgta aggaagcaat taactggcgg gtgatctcac catagttcac atcagtgcca
    39181 atttctgttg cggatcgcgc ccttccatca ctaacggtgc ccactctttg atctgcgcgt
    39241 gatcttcaga gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg
    39301 cgcgcaggaa attaacgtta tcctcgcccc agacaaagct catatgcgga acggtattga
    39361 taaatgaacg cggagtacgc agcacgccgc gctcgacctg gtgcgcccag aactggcggg
    39421 aaatctgaaa tgcttcgtta atggcgactg ctttttcaat actgatgctg ccatcggcgt
    39481 tttgcggggt gtagttcagt tccatcagtg cagaatgccc ggttccggcg ttgttctagc
    39541 cgttcgaact ctcctgcgcg cccccctcca ggcgctccac catggtcatc gaccattcag
    39601 gctccagctc gcgtcaatag gtccccaacg tggcgctcat aatgccgccg ccaatcaaca
    39661 gtacatcagt ttcctgctcc tcggacgctt tcgcttttgc cgccatcgaa acggcattaa
    39721 gccccacggc catcgagaag agcatggcag tcactttttt catcttgtta atgccttact
    39781 tttagtcgct ttattgcagg tgagatttgc gcggcatcaa cggtaacact taagtaacaa
    39841 catttaaata atgtttataa attatattct aattttagaa acattataat ttcgttgatt
    39901 aattataggg ttattagaaa tgaagggatt tttagcatat tgacctgacg gcagcagtcc
    39961 gccaggtcag cgtcgtaata ttatgccgtc cgggcaacgg catcacgcga agcggcatcg
    40021 cgctcttcgc ccgtcagctc gctaagttgc ccattgcgca tttccagcag gcggtcggcg
    40081 tggataaagt aatgatcatc atgactgata gcgaaaatag ttttacccat ctcctgcatc
    40141 agcggcagca acacctgata aaactcacga cggaagtgtg gatcctgatc caccgcccat
    40201 tcatccagca ggataatatc gcgttcttct gccagcgcca gcaacagcgc cacgcgtttt
    40261 ttctgccctt ttgataactt caggttaaca atacgcccgt tgcttaactc aagcttatga
    40321 gccattttca gctgcgccag ccacttctca accagttgcg ggttagcggg ttgcccctcc
    40381 ggccccagca gttgatcaaa cagccagaca tcggtaaaca ctgccgaaaa cagtttgcga
    40441 taatcttccg gttgttcgcc gctgacaggt ttgccatcca gcaagatttc gccgctttgt
    40501 ggctgataca agcccgtcaa caacatcgcc agcgtcgatt ttccgctacc gttaccgcca
    40561 atcagaaaca gcagctcgcc acgtttgatg gtgagattaa tcggaccaac ggaaaacgcg
    40621 ttatcctgat aagcaaacgt cacgttacgc agctccagcg tttgccagtt gggaaacgcc
    40681 tgcgggcgcg gaaactcagc tttgaaaggc gcgagcgcga atttgttcag cttgttaaac
    40741 gccacctgcg ccgtcagcag cgtcggcaat gcgccaaccg ccgaaagcag cggcgtacgc
    40801 aggaataaca gcgtcaacga ataggtcgca gcaacgttgg tatcagccca accgagactg
    40861 ttcgccatcc agaacaccag gccgatagcg cccagcatca tgatgtttga ccagttcacg
    40921 gcactaagat ggaaggtgtc tgcgcgaata atatgatggc gatactcttg cgcatcgggg
    40981 atgtagaggt tgttaaacac atactcggcg cgttcccggt tcagagtcag ctctttgcgc
    41041 ccttcaagta cagtttgaaa atccgtgtac agcttgtctt cggtttcacg tagggtcgcc
    41101 atatgtttgt acacccgcgc caccagcaca aaaccgcccc agatggtgat cgccatccag
    41161 atagccgtta ccagcaacat tttgcccgac agcatccaca gatacgccga tgaaccgata
    41221 gtgagaatga tcccctgccc cagttccggc agacgcacaa aagcaatggt gatattgcgc
    41281 acatcgctgg ttaatcccgc cagcaacgag gcgctaccga gttgttcaat gcgctcgacg
    41341 tgggtatcca gaatccgctt gataaattca ctacgcagtc ggtaaacgaa gtgatgcccc
    41401 aaagtggtga gcgccagttg cgatccgaga gtgactgcca tcaacagcag caataatccc
    41461 agaaactccg gtaacaccag cagactggta tccgccgttt cgataaggcg ctgattgata
    41521 aaagcaatta agccaatgcc taatgccgca ctggcgaggc ttagcgccat cacactgata
    41581 aatggccagc gatactgccg ccagacaagt acaagaagtt ccatgcagaa aacccggaca
    41641 atgaattaca gcccgcagtt taaacatctt cgcgcgcaca gcaataataa ttcttatttt
    41701 tattcttttt tacctgcctg acggaatgtc aggttgtagc ggcagtcggt ggtgagtgga
    41761 tgaaaccccg ctttcaacgg ttgaataccg tgataaaaca gccgcgattc accgccccat
    41821 accaccacat cgccatgttc caacaacaaa cgtttgagcg gatcattacg tttcaggcca
    41881 ccaaattgaa aaatcgcggg taagcccaga gaaacagaaa caattggcgc gcgcagatcc
    41941 ggttcgtctt tatcctgatg cagcgacagt ttcgcgccag gagcgtagcg gttgataaga
    42001 caagcatctg gctggaaatc tggatagccc gccgccgtag ccgcacgttg acataaatca
    42061 tgaaagcttt gtggcatagc gggccacggt ttatttgttt gcggatcaat gggcgaatag
    42121 agataacctt gccgatgggt cgtccagccc agatgcccac agttggtcat cgccaccgac
    42181 atggtatatc cgcctggagc gaccatctgg cgaaacggcg actggctggc aacgtcatta
    42241 atatcgcgga ttagttgctc cgcagcatta aaagcaaaac gccgtaaaat taccgcacca
    42301 gccgccagtg gctcttgcca cggttcagca tcggcaaaca gatccaacat tacctctcct
    42361 cattttcagc ttcgcggcgc agcagttgcg ctttacgcga cacgccccag cggtaaccgg
    42421 aaagtgtgcc atcaccacgg accacccgat gacagggtat tacgatagcc agcttgttgg
    42481 cggcacaggc gctggcaacg gcccgtaccg ctttcggttt gccgatggcg ttagccagtt
    42541 gctgataact gacggtttca ccacaaggta tcgtgcgcag tgcctgccag acttgttgct
    42601 gaaaagcagt gccgcgaata tccagcggta acgtcagcgg cgtatcgcgt tgattgaggc
    42661 tggcgatcac ttcacgcaca tgttgctgaa acgtcagatc ggcaggcgcg ttgtcggcag
    42721 cgggaaacat ctgctgcaac tcgctgatta atgtcgcgtc atcatcgccc agcaatatcg
    42781 cgcaaatccc ccgctcgctt tctgccacca ggcaacgacc cagctcacaa tcagccagcg
    42841 cgtaacgcac cgccagattt tcgccaccgt gacggaattg tttagccgtc atgcccagcg
    42901 tttcatcggc tttgcggtag taactgctgc tgtcggggaa tccggcgtta agaatagacg
    42961 tcgtcacgct ctcccctttc gccagcgatt cgcgcaaacg gcgagcgcgc caggcctgtt
    43021 gccaggcttt aggcgtcatt ccggtagtag ctttaaacaa ccgatgtaga tgaaatggac
    43081 tcatcgccac ctggtcggct aaggcttcca gcgttacagg cgtttcctgt tccagcagtc
    43141 gacacgcgtg ggtgatttta tccaaccgat gttgccgggg attggctttg tctggctgac
    43201 aacgtttgca ggggcgaaat ccggcggcga gtgcctcgct ggcatttgcg tagaaggaga
    43261 cattttcccg caaggcatgt ctggcgcggc aagaaggacg gcaaaagata cccgtggtgc
    43321 gcacggcgaa aacgaattcg ccgtcggcat tcgggtcgcg ggctaagaca gattgccagc
    43381 gttgatcgtc agttaagcat gtggcgtttt tcataatcag ctccctggtt aaggatagcc
    43441 tttagtctgc ccggtcacca tcacgcaaaa accaacaatc ttgcgcttta atttttttcg
    43501 ctgacaagga agcttttaaa ctgtggtgac atccaggttt taaagctatc gccttctttg
    43561 gtgatcatat aaaccgccag cccttcccgg cgaacaacct ctttggcttt ctccggcccg
    43621 agtaccatca agccagtatc ccaggcatcg gcttccagcg ccgtcggagc aatcaccgtc
    43681 acggatacca gattgtgttc gatgggacgc ccggtttgcg gatcgataac atgggaaaga
    43741 cgtttgccgt ccagttcgta atagttacgg tagctgccag aggtgctaat cccatgaccg
    43801 ttgatatcca ccacagcctg aaccgcgttt tctttatcgg ttggtttttg aatcgctacc
    43861 cgccacggct ggccttcacc gttcatacca cggctgttca gcgcgccgcc caccgacacc
    43921 agatagcggg aaatcccttc ctgctccatc aagcgtgcca ggtgatccgc cgcataacct
    43981 tcaccgacgg tggagagatc gacatataaa tccggcaggt ctttttgcag atattgctga
    44041 tgcgactgat tgatgaccgt caggtgctgt aagccggttt tggctttcat cgcatcgatc
    44101 tgttcctggc tcggaatttg aaccggctgt tgttccgggc caaagcccca cagattcacc
    44161 agcggcccga cggtaatatc catcgcacca tcggtccttg ccccaatgcg cagcgaggtg
    44221 gtgacgatat cggccatcgc ttcacttacc ggccacggcg acaaactttg cgagtcgtta
    44281 aagcgcatca gcgcggaatc ttttttatag gtcgaaagca gctgatcgtc ggcgtccagc
    44341 tgggtctgaa tcttttcttt aagttcggca ctgcgtttgg catcaatgcc cgggatgctg
    44401 gcacgccaga aggtacccat ggttttgcct tcaagaacgg taacttcagt agcgtgggtt
    44461 ttggcgggtt gtggtttttg atcgcaacca acaaagaaga gcgcggcaac cagcagtgcc
    44521 acgcgggtaa agcttatttc catacgtgat tatcctcatg cgaacggtcg caagagtaca
    44581 ccaaaaaact gtgtttgtac gctgaaaaca atgaaaaaag ggcccgcagg ccctttgttc
    44641 gatatccatc gagattagaa ctggtaaacc agacccagag ctacgatgtt atcagtgttg
    44701 atgccagcgt cacgagtgaa ctggttgtcg tccagcaggt tgattttgta gtcaacgtag
    44761 gtggacatgt ttttgttgaa gtagtaggtc gcaccaacat caacatattt caggatatct
    44821 tcgtcgtcgt agttacgacc attgatgaca cccaggtttt tacctttaga ctgcaggtaa
    44881 gccagggacg gacgcagacc gaagtcgaac tggtactgag caacagcttc gaagttctgt
    44941 gctttgttcg cccaacccaa gttgcctacg cgagttgcgt tgtaggtctg ggtgtactga
    45001 gcagctaggt agatgttgtt cgcgtcgtat ttcagaccac cggtgtaggt ttcagcgcgg
    45061 tcgccattac cgatgtaacg ctcatcataa cggttcagac cgaagttctg gtcatcagta
    45121 cgtttggagc tggaaactgc agcaccgata ccgaagcctt cgtaatcata agtaatagaa
    45181 ccgccaacgc cgtcaccgtt ctgacgcaga gcttcacgac cgttgttggt catgccttca
    45241 ccttccgggc taccgttttt gccctgatac tgaacagcga agttcagacc gtcaaccaga
    45301 ccaaagaagt cggtgctacg gtaggttgcg aagccgttac cacgctgctg catgaagttg
    45361 tcagaaccgt aagtgtcacc accgaattct ggcagtacgt cggtccagga agttacgtcg
    45421 taaactacgc cgtagttacg accgtagtcg aaagaaccta catcctggaa tttcagacct
    45481 gcgaatgcca cacgggtcca ggagttgttt tcgttttcag cgctgttgcc ctggatctga
    45541 tattcccact ggccgtaacc ggtcagctgg tcagtaacct gagtttcacc tttgaagcca
    45601 agacgcatgt aggtctggtc gccatctaca gacttgtcgt cagagaaata gtgcaggccg
    45661 tctactttac cgtacagatc taatttgttg ccgtctttgt tgtaaacttc agcagcgttt
    45721 gctgcgcctg ctaccagcag agctgggacc aggagggaca gtactttaac tttcatgtta
    45781 ttaaccctct gttatatgcc tttatttgct tttttatgcc actgcatact gattaaccct
    45841 cattaatcag tcggcaagtc cattctcccc aaaaatgcag aataatccaa cacgaatatg
    45901 atactaaaac ttttaagatg tttcatttat cgctatagac gtttcaaaat gtaaatacaa
    45961 gggaactttt taagattatt gcggaatggc gaaataagca cctaacatca agcaataata
    46021 attcaaggtt aaaatcaata acttattctt aagtatttga cagcactgaa tgtcaaaaca
    46081 aaaccttcac tcgcaactag aataactccc gctatcatca ttaactttat ttattaccgt
    46141 cattcatttc tgaatgtctg tttaccccta tttcaaccgg atgcctcgca ttcggttttt
    46201 ttacccttct ttacacactt ttcattattc tgtgctacca cagaaaaact ataacgcttg
    46261 ttaactattt cacaaataat taacatcagc ataatttcca gcaatctttg tttatttgca
    46321 attaatttcg ttgggctttt tgtaggttat ttgtacagca aaatggcgct tgtacatcta
    46381 tttcccccca tacaggatga taaatatcac gggagaatag agaatcatca atcaggtaag
    46441 agtctggaat ttcacactgt accctttata ctgccctatc acttcgcgaa gttttaacag
    46501 gtcataaaca cgaatgcgtc agaaagagac aacggccacg acccgctttt cactcctacc
    46561 ggggagcatt acccgcttct ttttactgtt gatcattgtg ttactggtga cgatgggggt
    46621 aatggtacaa agtgccgtta acgcttggct gaaagataaa agttaccaga ttgtcgacat
    46681 tacccacgca atccaaaagc gcgtcgatac ctggcgttac gtgaccaggc agatctacga
    46741 caacattgcc gcgacgacct ccccctcctc cggcgaaggt ttacaagaga cgcgcctgaa
    46801 acaggatgtc tactatctgg agaaaccacg ccgcaaaacg gaagcgttaa tctttggctc
    46861 tcacgacaac tcaacgcttg agatgactca gcggatgtcc acttatctgg atacattgtg
    46921 gggtgcagaa aatgtaccgt ggtcgatgta ttacctgaat ggtcaggata acagtctggt
    46981 gctgatctca accctacccc ttaaagatct cacctccgga tttaaagaat cgaccgtcag
    47041 tgacattgtt gattcacgtc gtgcagaaat gttacaacag gccaacgccc tcgatgaacg
    47101 cgaaagtttt tctaacatgc gccgcctagc ctggcagaac ggtcattact ttaccttgcg
    47161 taccacgttc aaccagccgg gacatctggc aacggtcgtg gcttttgatc tgccgattaa
    47221 tgatttgatc ccaccgggta tgccgctgga cagtttccgc cttgagccag acgcgacggc
    47281 aacgggaaac aatgataatg agaaagaagg gacggatagc gtcagtatcc actttaacag
    47341 tacgaagatt gaaatctcct cggcactcaa ctctaccgat atgcgcctgg tctggcaggt
    47401 tccttatggc accttattgc tggatacgtt gcaaaacatt ctgctgccac tgctgctgaa
    47461 catcggtttg ctggcgctgg cgttatttgg ctataccaca ttccgccatt tctccagccg
    47521 cagtacagaa agcgtcccca gcacggcggt caataacgaa ttgcgcattt tacgggcaat
    47581 caatgaagag atagtctcac tgttgccgct cggcctgctg gttcacgatc aggaatcgaa
    47641 ccgcactgtc ataagtaaca aaattgccga tcatttgctg ccgcatctga atctgcaaaa
    47701 catcaccacc atggcggaac agcatcaggg gattattcag gcgacgatca ataacgagct
    47761 gtatgagatc cgcatgttcc gcagccaggt cgcgccgcgc acacaaattt tcattattcg
    47821 cgatcaggat cgcgaagtgc tggtaaacaa gaaactcaag caggcgcagc gtctgtatga
    47881 gaaaaaccag caggggcgga tgacctttat gaaaaacatt ggcgatgcgc tgaaagaacc
    47941 cgcacagtcc ctggcggaga gtgcggctaa actcaacgcc ccggaaagca aacaactggc
    48001 gaatcaggca gatgtgctgg tgcggctggt cgaagaaata cagttagcga acatgcttgc
    48061 ggatgatagc tggaaaagtg agacggtgct gttctccgtg caggatttaa ttgatgaagt
    48121 tgtgccttca gtgttgcctg ccatcaagcg taaaggtctg caactgctga ttaacaatca
    48181 tctgaaagca cacgatatgc gccgcggcga tcgcgatgcc ttacgacgta ttttgctgct
    48241 actgatgcaa tatgccgtga cctcaacgca attgggaaaa atcacccttg aggttgatca
    48301 ggatgagtcc tctgaagacc gcctgacatt ccgcattctg gacacgggag aaggcgtaag
    48361 tattcatgaa atggataatt tgcacttccc gtttatcaac cagacccaaa acgatcgtta
    48421 tggaaaggcg gacccgctgg cattctggct gagcgatcaa ctggcacgta aactgggcgg
    48481 tcatttaaac atcaaaacgc gggatgggct tggtacacgc tactctgtgc atatcaaaat
    48541 gctcgccgct gacccggaag ttgaagagga agaagagcgt ttactggatg atgtctgcgt
    48601 aatggtggat gttacttcgg cagaaattcg gaatattgtc actcgcctgt tagaaaattg
    48661 gggtgcaacc tgtatcacac ccgatgaaag attaattagt caagattatg atatcttttt
    48721 aacggataat ccgtctaatc ttactgcctc tggcttgctt ttaagcgatg atgagtctgg
    48781 cgtacgagaa attgggcctg gtcaattgtg cgtcaacttc aatatgagca acgctatgca
    48841 ggaagcggtc ttacaattaa ttgaagtgca actggcacag gaagaggtga cagaatcgcc
    48901 tctgggcgga gatgaaaatg cgcaactcca tgccagcggc tattatgcgc tctttgtaga
    48961 cacagtaccg gatgatgtta agaggctgta tactgaagca gcaaccagtg actttgctgc
    49021 gttagcccaa acggctcatc gtcttaaagg cgtatttgcc atgctaaatc tggtacccgg
    49081 caagcagtta tgtgaaacgc tggaacatct gattcgtgag aaggatgttc caggaataga
    49141 aaaatacatc agcgacattg acagttatgt caagagcttg ctgtagcaag gtagcctatt
    49201 acatgaacaa tatgaacgta attattgccg atgaccatcc gatagtcttg ttcggtattc
    49261 gcaaatcact tgagcaaatt gagtgggtga atgttgtcgg cgaatttgaa gactctacag
    49321 cactgatcaa caacctgccg aaactggatg cgcatgtgtt gattaccgat ctctccatgc
    49381 ctggcgataa gtacggcgat ggcattacct taatcaagta catcaagcgc catttcccaa
    49441 gcctgtcgat cattgttctg actatgaaca acaacccggc gattcttagt gcggtattgg
    49501 atctggatat cgaagggatc gtgctgaaac aaggtgcacc gaccgatctg ccgaaagctc
    49561 tcgccgcgct gcagaaaggg aagaaattta ccccggaaag cgtttctcgc ctgttggaaa
    49621 aaatcagtgc tggtggttac ggtgacaagc gtctctcgcc aaaagagagt gaagttctgc
    49681 gcctgtttgc ggaaggcttc ctagtgaccg agatcgctaa aaagctgaac cgcagtatta
    49741 aaaccatcag tagccagaag aaatctgcga tgatgaagct gggcgtcgag aacgatatcg
    49801 ccctgctgaa ttatctctct tctgtgacct taagtccggc agataaagac taatcatctg
    49861 taggccagat aagacgcgtt agtgtcttat ctggcatttg caccgattgc cggatgcggc
    49921 gtaaacgcct tatccggcct acgattccca ttatttcaac agattacatt aaagtaggcc
    49981 agataagacg cgtcagtgtc ttatctggca tttgcactga atgccggatg cggcgtaaac
    50041 gccttatccg tcctacgaat cccgcgattt cctgaccctc tcggcatata ccgtcagcgt
    50101 ctgttttatc acatccagcg ttaccggctt cgagaggcag ctgtccatac cggactccag
    50161 acaccgctgc ttctcttcag ccaacgcatt agcagttact ccgattaccg gcaacgtcag
    50221 tcccaactga cgaatgcgtt gcgtcaagcg gtaaccatcc atatttggca tgttgacgtc
    50281 gctaagcacg atatcaatat gattcttact aagtacatta agcgcatcga cgccatcatt
    50341 cgcggtttta cattgatagc ccaacgatcc caactgatct gccagcagac gtcggttaat
    50401 cggatgatca tccacgacca gaatcatcat atcgtcgtta tcgctgaccg ctttgtccgt
    50461 cgacggcaga gcgttagcag gatcgtcgct ctccatctcg atcaaataaa tacgcgccaa
    50521 caatgccggt agctcatgcg gagcagccac actgtgtacc cactcccctg gcgctttctc
    50581 cagcggaata ccaatatggc gacgacagaa ggtcactacc gctctgccct gccatttttt
    50641 actcactacc tcgtcagtga ttaacacatc ttcgggagtc ggttcctgcc cttcgtatgt
    50701 tgtaacgacg atgccgctgc gctgcaaact ggtttccagg aactgacaga gcgacgcatt
    50761 gcggaccgcc agccagcagc gtttaccact caacccttcc acgccttttt tctgcgggta
    50821 ctgagcgccg tacaacggaa tacgcacggt aaactggctg cccattcccg gttctgaatc
    50881 taccgagata tcgccgtcca tcatgctgat cagtttttca caaatcgcca gacccagacc
    50941 ggtcccctgg aagtttcgct gtacgcccgt tccaacctgg aagaagggat caaacaagcg
    51001 caccacttct ttcgccggaa tccccacgcc ggtatcgcga acacggatag agagataatc
    51061 gccatcagcg cgaacatgca aaactataca gccggtatcg gtgaatttta tggcgttact
    51121 caacaggttg gagatgacct gctgtaaacg catcgggtcg ccatttaagg ccactggcac
    51181 atccggttca ataaagcagt acaagcctaa ctgcttgcgt accaccagcg gtaaatagtt
    51241 ggcggtgatg tggttcatca cttcacgcgg tgaaaactca cgcggttcga tcttcaactg
    51301 ttccgattca atcttcgaga aatcgagaat atcgctgata attttcaaca acaggctgga
    51361 agagttgttc attgccgtca ccagacgatc gacgcctttc ggtaactctt tggtttgcaa
    51421 cagatccagg ttaccgataa tgccatacag cggcgttcgc agctcatgac tgacggtggc
    51481 aaggaacatc gattttgact ggctcgcctg ttccgctgct tgtgccatct cctgcaacga
    51541 ctcttccatc ttcacgcgcg aagaaacatc caccagcaca caaatggcca cgttttcatt
    51601 acgatagcgc gaatggacga agctgatttg cagattggta ttgttgctgg tcaggacatc
    51661 aacaaaattg acctgctgcc cacagataat ttgcgtcagt cgttggcggt cctcatgcgt
    51721 aagcatattg agataggtat gcgccagttc gttacttaaa atattgacgc catcagcggt
    51781 acgcaaaatg cagataccca ctggcgcgga ggcgacaatc ttgcgattga actgctcatg
    51841 ttcttccagt cgcagggcgt cgctttccgc cggaatgaaa atacgtcgct cgtacatccg
    51901 cgcgagagta aacaatgcag ctccggcaag cacattcagc aaaattgcgt taaggatcaa
    51961 catgcgaatg cgttccagca ccttatcaac cggcaccgaa tacacgatgc ttagcgatga
    52021 gggtggcaga tttttcttca gcaccagctc ccggaaccct tccgtatagc caaaccagga
    52081 gcgttcctgc atccagcgag gatcgccctt aattttactt tctggtccgg taagcgaaat
    52141 cagggtatga ccattttcat caagaatggt aacccccatc ggcaacgtac ccggtaagaa
    52201 aaagttctcc atccggatgg tctgctcgac acccaaaagc gcctgcaacc ggttcgccag
    52261 ataaactggc gtcaacgcgt aaaaataccc gacgcaaggg cgcggacctt cgctgatcca
    52321 gtagaggtta ctgccgctat catcttgtgg tgcatttcga tatttattga tgcgttcatg
    52381 caacgctttc aacgcggtat cgcgttccac tggcatatca cgcagaccga aattggccat
    52441 gcagaggtta tcactgccga ttaaaaatac ccggttgaga tcgtaagccg cagaaaaatt
    52501 atcgcgccag tagcgcataa accacgccaa tgactccaga gaacctcgcc aggtgttact
    52561 cattgcggaa caatcggagt cggcaaacag cggttcaaac gcaggcacat ccgcctgcgt
    52621 ttctcgtcca cgcggggaaa gcacaccgtt ttctgccgat aagcgatttt cggcgatgta
    52681 cttaagctct ttcatcacat cagaggtgcg ttgaataaag cgctgagcct gatcggaact
    52741 cagattaaat tcctgacgaa tttccgattc tcgctgatgt aacgcattaa cgatgtaaaa
    52801 aacggatgaa aaagcaatca acagccagag cactaacgcc aatgctctga acatgtagcg
    52861 cgaggctttc agggttgtac gaaaagaagc aaggtatttc aaaggggaga agctccgcct
    52921 caggtgaccg atggagtgtg gttaaggtag cggtaaaagc gtgttaccgc aatgttctgt
    52981 caccattgca ataatgcgta tcgcttagaa accattgcct taaccaggaa agccggagta
    53041 gatggacagt tctcagtacg aaaaataaga aatgaccaat ctgccgacgc ttgttaatgg
    53101 ttatatcctg gcgtcggcag atcgcccagc agtcttatct gaaaaaagag agagccaggt
    53161 tgtgtgaagg gatggatcac cagtcaaaac ttttcaccag cgtcagctcg ccagcattac
    53221 gcatcggtac aataaatgtt tcctgtttct cattgaccga tccttcatcg gtgatcagcg
    53281 tcagttgggc ggtcgtcaat tccgtttcgc tgcgcccacc atagtagttg atatacacct
    53341 gatagcgccc gtgaactggt gctggcatgg cgaaaatctc gggtccgtac cccgtcgtga
    53401 catccatatc cagtgcacca ctgtttttca gcacggtgtt accgtaccag gcgtgttcgc
    53461 catcgggcgt gacaacatgg agatcgagat cggtattgtc cgtatcccac gagagaacca
    53521 gccgtaaacg tgcacgaata gttcctgtac ccggcgttga gtaaaactgc attttttgcc
    53581 ggctttgccc atccgggctg atgacctgca cgctgttgct gccttcagtg aaaatataag
    53641 ggcgtgcaaa tgaaccatcg gattcaattc tctgcggcat actggcaccg ttgaccacca
    53701 gccggccctg ctgaacttta cccgccgcag cataattctt tattttgccg cggatctgcg
    53761 ctgagatatt ttgatcatct gccatgttga cggacgacgc cgggtaattg atcgattgtg
    53821 aaaaactggc atcttcgccc tctgccggat gccagccaga gagcggtgcg tcaatctcga
    53881 cttcctgcac accttcactg tgagcgacag gcgaaagtgc caccagtaac aacggaagaa
    53941 aaatctttcg catagcatta gtccattaat aattgtcggg tcagcgtttc gatatagttc
    54001 tcatcaagcc cggtgggata accatcaaag gccaagtgca gatactcatg ggttaaatcc
    54061 agccgatcct gaagcgtaaa gaagttacga ataaacagcc gtttctgctg ccgatcggta
    54121 taggggaaac ctgagactaa acgacagacc gcaaacacgt ctggttcgtt gtaccccgtc
    54181 tcgccttgta atatacgccg ccactgcggc atttttttcg ccagccaggc tttcgctttg
    54241 ggtaataact ggcaggttga gcgtggagct ccccagcggc taaggctgtt gtcgggataa
    54301 gcaaacgcga gtatctggtc gtaacgctcc ccctgccccg cctgggccgt ggcttgccgc
    54361 caggaaagtg tcccttcggt agcgcgactg ccatgataat gaaccggatc gcctgcatag
    54421 atgagctcct gcgtccaggc ggtcatcgtt cgcgcccccg tggtcgctgg cgaagcagaa
    54481 acacgctgcg tggcgctgct gtcggggatg gtcaggcaat caccttcccg attggcgttt
    54541 tgctgtaaaa aagtacgtat cgccacagtc atggctttcg ctgcctcagg tggcgtactt
    54601 ttcgcctcgc gatccagaac tctggcaacg tactcttcac gatcgagatg cgactgcaat
    54661 ttcagtttgc ccttttcgct cagtaaggtc gtctcaccgt ggctgacaaa cgtaatgtgg
    54721 ttaccattag taaacgtcac acgatagcgc ccattaagta cgctgggttt tactgccgtc
    54781 gtactctttt ccgctgtgat tttcttcagc ggatagcggg caaacagctc cacctctaca
    54841 cattgcccac tggcaacctg cgtcggcact ggcaaaaccc gattaagcac cgtcgcataa
    54901 cgggttagta ccgttttgct ggtgccactg ccagtaaccc acagcggtgt accatcggtt
    54961 aaccagccag caaaaccacc ctgacggatt tcctgtttat catcggcaaa ccagctccag
    55021 gttttcaccc gtacccgact accgagcatg gatgcgacac cgattttcgc ctcgtccagc
    55081 accacatcca gtaagacttc ctgagctttg ttttgcgcag gtaacgtcgc taatgaatca
    55141 agcagcgatt taaccgttac cgaggtttct ggcttcagcg ttgtaagaga cgctaaccac
    55201 gcaggtgcct gacgctgttg ccagtattgc ccccacacat ctgcgccaat atgcaaccgc
    55261 tgcggcgcaa aatagagccc acaggaacgt accagcgcgg tatcgcgcgt tatgctctcc
    55321 ccagcctgac aacaataaac ctcttccggg gagtttccgc gacactggta gggctgctcc
    55381 ggttgatggg tatcctccag ccaggcgtaa acatacagtt tccacaaact ccctaacggc
    55441 gtggtgagcg agtcaggtaa tgctgagact ttggtcacgc ctgatgacga cagctgatac
    55501 agttgatcgt gctgcgcacc gcgcagcacc agttgtaacg gcgtctcctc cgcaagcgtt
    55561 ggcagagtaa ccaacgccaa tagccagacg attctgcgcc agttcactta tttcacctgc
    55621 atcccggtcc attcactccc tgccgcgaca ctttgctgcg caggtgcata ggaacgcaca
    55681 taacgagcag gaggcagaac gaattgccct ttttgcgaga agcgcagcaa atggcggaaa
    55741 gtgaccgttc ccgtcagttc tttcaccggc accatatagg ccagttcgcc catttcatta
    55801 cgcgcttttt ccagcaattg cccctgctgt ttcgcggcgt tgggtttatt gactgaaatg
    55861 ccccatgttg tgcgctcaac gtcagctccc ggcgggagcg gtacttccac ctgaccatag
    55921 cgcagaactg catcctgctc gctggtaagc gtaatttcat cgagatacag cgcatcgctg
    55981 tcaatctcat tgctggtcac cggctgcaga gtaaagctca tctcttcttc accggggata
    56041 agccgataca actggcgttc aacggtcacc ggaatgttac tttgttgagc cgtttttgcc
    56101 ggttcacgcc agcggacctg cacatttgcg gcgataattc gtcaccaaaa gagagaatgt
    56161 ccggcacacc ctaaccaacc caacgccagt cttcgccccc tccagttaac ttatgtttag
    56221 cccatgcgcc cgcaggcgca ggcaaaacaa ctggaggcat tgtcgccata tatttcgcca
    56281 gccagttcat ggccagcgcg cgctcaatag tggattgctc agcggtcaaa ccattaaaat
    56341 agcggccgtc tgggtagcgt caccaccgct attgagcagc acaaccgtgt gcgccagcgg
    56401 ctgattgctg tttgccgctt tatcccacgc atattgctga acctgtggca tcaccgtgga
    56461 cttcaactgc gcttttttca gcaacgtcat ggttaagacg tttgccaccg catctgccag
    56521 tggagattcc ggtgtatcga ggatcaggct gtcattgata tcgcggatgt cttcctcaga
    56581 gaaatcttca tctttagttc cgcgccgggc gatagcttca tccccccctt tcaacaacgt
    56641 tttgcacggc agattcatct cctgtgccca cgccagcacc agcgcccgat gcaataacgg
    56701 catattatcc gcctgctcgg cataactgtc gagcatatgc tgccagtatt ccggttgttg
    56761 cgttacgccg agcgcctggc tggcctgcca gtcggcgtac catgcccatg ccgtaaggaa
    56821 ggcgtcacca ttgccatctt caccccacca ggtaaagcgc gctccgggcc ccgccagttg
    56881 catcagccgc agacggttat cctgaatcat ctgacgaatg tcgttagcgg cggcactttg
    56941 atgatcggca agcgaacgcc aggcgagact gagcgggatc agacggctac cggtgttgat
    57001 caccccaccc cacggttcat cgactaacgc atcaagattg ttgcggaaaa tctcctgcgg
    57061 cgtttcacta ctttgtagcc ggatattact cgcctgctcg ggcaacatca gcgcgttctc
    57121 gccaccgccg agcatgacat ttttctgctg ttcaacgggc cagctgttgt ccacaaaaga
    57181 cagttttgtg ctaatgctgt cctgcacttg cccattttgt tgcagttctg cacttaacaa
    57241 gccagattgc tgaatattct gcgtcagcga aatataattc gcccctttgt gcagcgtcag
    57301 cgtctggcgc atctcagcgc ctgcaaattt agtcaccagc gccaccggtt cgttatcctg
    57361 ctgactgaag ataaacagtc ctgccgccgg tttgtcgccc acgcgataca ccgttggcat
    57421 actccacttc atgtagagat ttttttccga acgcagataa gcacgcccct gcccgaccag
    57481 cccgtcgccg ttcatcccac gcgcggtgat acgccagcgg gttaacgaat caggcatcag
    57541 gaacgtgaaa tacgctttgc cttgtttatc ggttgtgagt gacggcatcc atgccgcggt
    57601 atccacctct tcacgccgtg gacgttcaag catttttact cgccgctcgc tgcggttagt
    57661 cgcgccaggc gcaaccggct cgctggagag cgcctggtca tagctgataa acgacagact
    57721 ggagctggta cgcacattgt tacgccccag cggatagaaa aatttgccga tattcggcgc
    57781 gatttctggt tgcagcgcgt agatcatttc atcgaccacg cctaccgtta gctgcgcaga
    57841 aacaggttta cctttcagcg acgaggttaa t
//
LOCUS       DAJTYH010000011        55238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTYH010000011 DAJTYH010000000
VERSION     DAJTYH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO609_000684"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO609_000684"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6632214.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO609_000685"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO609_000685"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6632215.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO609_000686"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO609_000686"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6632216.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO609_000687"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO609_000687"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6632217.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO609_000688"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO609_000688"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6632218.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO609_000689"
     CDS             7826..9559
                     /locus_tag="OO609_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6632219.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO609_000690"
     CDS             9708..10559
                     /locus_tag="OO609_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6632220.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO609_000691"
     CDS             complement(10546..10887)
                     /locus_tag="OO609_000691"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6632221.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO609_000692"
     CDS             complement(10889..11767)
                     /locus_tag="OO609_000692"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6632222.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLTPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO609_000693"
     CDS             complement(11733..14030)
                     /locus_tag="OO609_000693"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6632223.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO609_000694"
     CDS             complement(14081..14401)
                     /locus_tag="OO609_000694"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6632224.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO609_000695"
     CDS             complement(14416..15495)
                     /locus_tag="OO609_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6632225.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO609_000696"
     CDS             15804..16907
                     /locus_tag="OO609_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6632226.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO609_000697"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO609_000697"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6632227.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO609_000698"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO609_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO609_000699"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO609_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO609_000700"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO609_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO609_000701"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO609_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6632228.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO609_000702"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO609_000702"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6632229.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO609_000703"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO609_000703"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6632230.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO609_000704"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO609_000704"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6632231.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO609_000705"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO609_000705"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6632232.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO609_000706"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO609_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6632233.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO609_000707"
     CDS             30120..30728
                     /locus_tag="OO609_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6632234.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO609_000708"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO609_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6632235.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO609_000709"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO609_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6632236.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO609_000710"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO609_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6632237.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO609_000711"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO609_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6632238.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO609_000712"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO609_000712"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6632239.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO609_000713"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO609_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6632240.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO609_000714"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO609_000714"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6632241.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO609_000715"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO609_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6632242.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO609_000716"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO609_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6632243.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO609_000717"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO609_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO609_000718"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO609_000718"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6632244.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO609_000719"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO609_000719"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6632245.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO609_000720"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO609_000720"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6632246.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO609_000721"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO609_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6632247.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO609_000722"
     CDS             complement(44843..45142)
                     /locus_tag="OO609_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6632248.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO609_000723"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO609_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO609_000724"
     CDS             45895..46494
                     /locus_tag="OO609_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6632249.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO609_000725"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO609_000725"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6632250.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO609_000726"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO609_000726"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6632251.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO609_000727"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO609_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6632252.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO609_000728"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO609_000728"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6632253.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO609_000729"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO609_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6632254.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO609_000730"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO609_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6632255.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO609_000731"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO609_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6632256.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO609_000732"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO609_000732"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6632257.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..>55238)
                     /gene="yiiM"
                     /locus_tag="OO609_000733"
     CDS             complement(54612..>55238)
                     /gene="yiiM"
                     /locus_tag="OO609_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6632258.1"
                     /translation="EGKPSAIAKIQVDGELMLTELGLEGDEQAEKKVHGGPDRALCHY
                     PREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGDIFRWGEALIQVSQPRS
                     PCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPLELVSRVSDVTVQEAAA
                     IAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFSRRLWGK"
BASE COUNT        13448 a        14972 c        14125 g        12693 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcggggtca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttc
//
LOCUS       DAJTYH010000012        53566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTYH010000012 DAJTYH010000000
VERSION     DAJTYH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..256)
                     /locus_tag="OO609_000734"
     CDS             complement(<1..256)
                     /locus_tag="OO609_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6632259.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGV"
     gene            471..1199
                     /gene="tonB"
                     /locus_tag="OO609_000735"
     CDS             471..1199
                     /gene="tonB"
                     /locus_tag="OO609_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6632260.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1239..1637)
                     /gene="yciA"
                     /locus_tag="OO609_000736"
     CDS             complement(1239..1637)
                     /gene="yciA"
                     /locus_tag="OO609_000736"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6632261.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1742..2281)
                     /locus_tag="OO609_000737"
     CDS             complement(1742..2281)
                     /locus_tag="OO609_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6632262.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2311..3054)
                     /locus_tag="OO609_000738"
     CDS             complement(2311..3054)
                     /locus_tag="OO609_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6632263.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3082..3171)
                     /locus_tag="OO609_000739"
                     /pseudo
     CDS             complement(3082..3171)
                     /locus_tag="OO609_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3411..4049
                     /gene="ompW"
                     /locus_tag="OO609_000740"
     CDS             3411..4049
                     /gene="ompW"
                     /locus_tag="OO609_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6632264.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4109..4615)
                     /locus_tag="OO609_000741"
     CDS             complement(4109..4615)
                     /locus_tag="OO609_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6632265.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4661..5161)
                     /locus_tag="OO609_000742"
     CDS             complement(4661..5161)
                     /locus_tag="OO609_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6632266.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5247..5426)
                     /locus_tag="OO609_000743"
     CDS             complement(5247..5426)
                     /locus_tag="OO609_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6632267.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5806..6612)
                     /gene="trpA"
                     /locus_tag="OO609_000744"
     CDS             complement(5806..6612)
                     /gene="trpA"
                     /locus_tag="OO609_000744"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6632268.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6612..7805)
                     /gene="trpB"
                     /locus_tag="OO609_000745"
     CDS             complement(6612..7805)
                     /gene="trpB"
                     /locus_tag="OO609_000745"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6632269.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7817..9178)
                     /gene="trpCF"
                     /locus_tag="OO609_000746"
     CDS             complement(7817..9178)
                     /gene="trpCF"
                     /locus_tag="OO609_000746"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6632270.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9179..10774)
                     /gene="trpD"
                     /locus_tag="OO609_000747"
     CDS             complement(9179..10774)
                     /gene="trpD"
                     /locus_tag="OO609_000747"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6632271.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10774..12336)
                     /gene="trpE"
                     /locus_tag="OO609_000748"
     CDS             complement(10774..12336)
                     /gene="trpE"
                     /locus_tag="OO609_000748"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6632272.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12358..12451)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12428..12472)
                     /gene="trpL"
                     /locus_tag="OO609_000749"
     CDS             complement(12428..12472)
                     /gene="trpL"
                     /locus_tag="OO609_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6632273.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12610..13491
                     /gene="yciV"
                     /locus_tag="OO609_000750"
     CDS             12610..13491
                     /gene="yciV"
                     /locus_tag="OO609_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6632274.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13488..14108
                     /locus_tag="OO609_000751"
     CDS             13488..14108
                     /locus_tag="OO609_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6632275.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            14209..15081
                     /gene="rluB"
                     /locus_tag="OO609_000752"
     CDS             14209..15081
                     /gene="rluB"
                     /locus_tag="OO609_000752"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6632276.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(15121..15711)
                     /gene="cobO"
                     /locus_tag="OO609_000753"
     CDS             complement(15121..15711)
                     /gene="cobO"
                     /locus_tag="OO609_000753"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6632277.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(15708..16466)
                     /locus_tag="OO609_000754"
     CDS             complement(15708..16466)
                     /locus_tag="OO609_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6632278.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16686..17735
                     /gene="sohB"
                     /locus_tag="OO609_000755"
     CDS             16686..17735
                     /gene="sohB"
                     /locus_tag="OO609_000755"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6632279.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17771..18022)
                     /locus_tag="OO609_000756"
     CDS             complement(17771..18022)
                     /locus_tag="OO609_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6632280.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18402..20999
                     /gene="topA"
                     /locus_tag="OO609_000757"
     CDS             18402..20999
                     /gene="topA"
                     /locus_tag="OO609_000757"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6632281.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21209..22183
                     /gene="cysB"
                     /locus_tag="OO609_000758"
     CDS             21209..22183
                     /gene="cysB"
                     /locus_tag="OO609_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6632282.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22478..22642
                     /locus_tag="OO609_000759"
     CDS             22478..22642
                     /locus_tag="OO609_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6632283.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            22645..22812
                     /locus_tag="OO609_000760"
     CDS             22645..22812
                     /locus_tag="OO609_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632284.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22926..23021
                     /locus_tag="OO609_000761"
     CDS             22926..23021
                     /locus_tag="OO609_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632285.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23185..25860
                     /gene="acnA"
                     /locus_tag="OO609_000762"
     CDS             23185..25860
                     /gene="acnA"
                     /locus_tag="OO609_000762"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6632286.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(25924..26514)
                     /gene="ribA"
                     /locus_tag="OO609_000763"
     CDS             complement(25924..26514)
                     /gene="ribA"
                     /locus_tag="OO609_000763"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6632287.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            26684..27448
                     /gene="pgpB"
                     /locus_tag="OO609_000764"
     CDS             26684..27448
                     /gene="pgpB"
                     /locus_tag="OO609_000764"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6632288.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            27597..27905
                     /gene="lapA"
                     /locus_tag="OO609_000765"
     CDS             27597..27905
                     /gene="lapA"
                     /locus_tag="OO609_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6632289.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            27912..29081
                     /gene="lapB"
                     /locus_tag="OO609_000766"
     CDS             27912..29081
                     /gene="lapB"
                     /locus_tag="OO609_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6632290.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            29274..30011
                     /gene="pyrF"
                     /locus_tag="OO609_000767"
     CDS             29274..30011
                     /gene="pyrF"
                     /locus_tag="OO609_000767"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6632291.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            30011..30337
                     /gene="yciH"
                     /locus_tag="OO609_000768"
     CDS             30011..30337
                     /gene="yciH"
                     /locus_tag="OO609_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6632292.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(30463..30681)
                     /gene="osmB"
                     /locus_tag="OO609_000769"
     CDS             complement(30463..30681)
                     /gene="osmB"
                     /locus_tag="OO609_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6632293.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(30950..31699)
                     /locus_tag="OO609_000770"
     CDS             complement(30950..31699)
                     /locus_tag="OO609_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6632294.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(31789..31962)
                     /locus_tag="OO609_000771"
     CDS             complement(31789..31962)
                     /locus_tag="OO609_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632295.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            complement(32110..34084)
                     /gene="pdeR"
                     /locus_tag="OO609_000772"
                     /pseudo
     CDS             complement(32110..34084)
                     /gene="pdeR"
                     /locus_tag="OO609_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            34138..34241
                     /locus_tag="OO609_000773"
                     /pseudo
     CDS             34138..34241
                     /locus_tag="OO609_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34319..36253)
                     /gene="rnb"
                     /locus_tag="OO609_000774"
     CDS             complement(34319..36253)
                     /gene="rnb"
                     /locus_tag="OO609_000774"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6632296.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(36321..37448)
                     /locus_tag="OO609_000775"
     CDS             complement(36321..37448)
                     /locus_tag="OO609_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6632297.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(37592..38380)
                     /gene="fabI"
                     /locus_tag="OO609_000776"
     CDS             complement(37592..38380)
                     /gene="fabI"
                     /locus_tag="OO609_000776"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6632298.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            complement(38748..39101)
                     /locus_tag="OO609_000777"
     CDS             complement(38748..39101)
                     /locus_tag="OO609_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6632299.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            complement(39169..39975)
                     /gene="sapF"
                     /locus_tag="OO609_000778"
     CDS             complement(39169..39975)
                     /gene="sapF"
                     /locus_tag="OO609_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6632300.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(39977..40969)
                     /gene="sapD"
                     /locus_tag="OO609_000779"
     CDS             complement(39977..40969)
                     /gene="sapD"
                     /locus_tag="OO609_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6632301.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(40969..41859)
                     /gene="sapC"
                     /locus_tag="OO609_000780"
     CDS             complement(40969..41859)
                     /gene="sapC"
                     /locus_tag="OO609_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6632302.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(41846..42811)
                     /gene="sapB"
                     /locus_tag="OO609_000781"
     CDS             complement(41846..42811)
                     /gene="sapB"
                     /locus_tag="OO609_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6632303.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(42808..44451)
                     /gene="sapA"
                     /locus_tag="OO609_000782"
     CDS             complement(42808..44451)
                     /gene="sapA"
                     /locus_tag="OO609_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6632304.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(44764..45009)
                     /locus_tag="OO609_000783"
     CDS             complement(44764..45009)
                     /locus_tag="OO609_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6632305.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(45143..46528)
                     /gene="puuP"
                     /locus_tag="OO609_000784"
     CDS             complement(45143..46528)
                     /gene="puuP"
                     /locus_tag="OO609_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6632306.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            complement(46518..46682)
                     /locus_tag="OO609_000785"
     CDS             complement(46518..46682)
                     /locus_tag="OO609_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632307.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            complement(46831..48249)
                     /gene="puuA"
                     /locus_tag="OO609_000786"
     CDS             complement(46831..48249)
                     /gene="puuA"
                     /locus_tag="OO609_000786"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6632308.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            48461..49225
                     /gene="puuD"
                     /locus_tag="OO609_000787"
     CDS             48461..49225
                     /gene="puuD"
                     /locus_tag="OO609_000787"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6632309.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            49252..49809
                     /gene="puuR"
                     /locus_tag="OO609_000788"
     CDS             49252..49809
                     /gene="puuR"
                     /locus_tag="OO609_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6632310.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            49959..51446
                     /gene="puuC"
                     /locus_tag="OO609_000789"
     CDS             49959..51446
                     /gene="puuC"
                     /locus_tag="OO609_000789"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6632311.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            51448..52728
                     /gene="puuB"
                     /locus_tag="OO609_000790"
     CDS             51448..52728
                     /gene="puuB"
                     /locus_tag="OO609_000790"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6632312.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            52766..>53566
                     /locus_tag="OO609_000791"
     CDS             52766..>53566
                     /locus_tag="OO609_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6632313.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAK"
BASE COUNT        13427 a        13446 c        13867 g        12826 t
ORIGIN      
        1 agactcctgc cgctacgtac gggtcagcat cggcccaggc ctgagctgct tccagcgatt
       61 caaattcagc aataacggtt gagccagtaa atcccgcagc ccctggatcg ttactgtcta
      121 ccgctggcat tggaccagct gtcaacaaac gaccttcatc atgcagtaac tgtaaacgtg
      181 ctaaatgtgc cggacgaacg gaaaggcgtt tttcgaggct atcagctttg tcttgagcgt
      241 aaataacata caacacgggc acaactcctt gttcgggaaa gttgtaaagt acgttatttg
      301 aaagggcgaa gatctgcaac ggaaagatga tgtctttgtt aaggtcatgc ataaagtaag
      361 ggtaattacg ccaaaaatga cattttcact gatcctgatc gtcttgcctt attgaatatg
      421 attgctattt gcatttaaaa tcgaggccta gtttttctac tgaaatgatt atgacttcaa
      481 taacccttga tttacctcgc cgcttcccct ggccgacgtt actttcggtc tgcattcatg
      541 gtgctgttgt ggcggggctg ctctatacct cggtacatca ggttattgaa ctacctgcgc
      601 ctgcgcaacc aatttctgtc acgatggttg cgcctgctga tctcgaaccg ccacaagccg
      661 ttcagccgcc accggagccg gtggtagagc cagaaccgga acctgagccg atccccgaac
      721 cgccaaaaga agcaccggtg gtcattgaaa agccgaagcc gaaacctaag ccaaaaccga
      781 agccggtgaa aaaggtacag gagcagccaa aacgcgatgt caaacccgta gagtcgcgtc
      841 cggcatcacc gtttgaaaat acggcacccg cgcgccccac atcaagtacg gcaacggctg
      901 caaccagcaa gccggttacc agtgtggctt caggaccacg cgcattaagc cgtaatcagc
      961 cgcagtatcc ggcacgagca caggcattgc gcattgaagg gcaggttaaa gttaaatttg
     1021 acgtcacgcc ggatggtcgc gtggataacg tacaaatcct ctcagccaag cctgcgaaca
     1081 tgtttgagcg tgaggtgaaa aatgcgatgc gcagatggcg ttatgagccg ggtaagccag
     1141 gcagtgggat tgtggtgaat atcctgttta aaattaacgg caccaccgaa attcagtaag
     1201 cagaaagtca aaagcctccg accggaggct tttgactatt actcaacagg taaggcgcga
     1261 ggttttcctt caggatcaac cgctacatac ttaaataatg cttctgtcgc tttatagcgt
     1321 tgcccgattg gttcagacgc tacttttttc acccacactt caatattaat gctgaccgat
     1381 gtcgtccctt tctggacaca gcgtgcatag cagcacacca catcgccgac cgcaaccggc
     1441 cgtaagaaag tcattccttc aacccgcaca gtcactacgc gaccgtgggc aatttctttt
     1501 gccagaatag cgccgccaat atccatttgt gacattaacc aaccaccaaa gatgtcacca
     1561 ttggcattgg tatcggcggg catggctaaa gtacgtaaaa caagatcgcc ctgagggacg
     1621 ttatgtgttg tagacatggt aaaaccgact taaaaggaaa atcacaggcg cgatgctact
     1681 atgattttcc tgaggagaac agaggagata cagcgccagc cccgaaggac tggcagtctg
     1741 gttaggattt atcttcctgc ggcatgtggc ggtagatata gataccgctt aacaatgtga
     1801 agattaaggt aagggcggtc aggccaaaga ctttaaagtt gacccaaata ttttgcggca
     1861 gccagaatgc gatgtagatg tttgccagac cgcaaaggat aaagaaaaca gcccaggcca
     1921 gatttagctt cgaccatacc ggttgcggca gcgtgagttc tttactcagc atccgctgaa
     1981 ttagcggctt tttcatcacc cattggctga ctaacagggc tcccgcaaac agagcataaa
     2041 tgactgtaac cttccattta ataaactcat cattgtggaa gaacagcgtc aagccaccga
     2101 agacgaccac cagaacaaaa gtgatcaggg ccatcttctc aaccttacga aagcgaaccc
     2161 agctatagat aagcacaatc gccgtggcga cgatcagcgc tgcagtagcc gcatagatgt
     2221 cataaatctt gtaaaacgcg aaaaagacaa ccagcggtaa aaaatcaaga aactgcttca
     2281 ttttacgatt ccgtaatcaa atgcttaagg ttattggcga atcaacatat acaggcggaa
     2341 cagatagatg agcaatatgg cagaaatcag gttgctcaag gtgttcgcca gtacggcacc
     2401 aatttccggg gttaatgcgg caaaagaaga ggcaaaaagc agcaacagtg tttttgccag
     2461 caaccagctc agtactgcgg gtgccaccag acgcatattc gcccaagtca gccgcatact
     2521 gctacgcatc gaggcaaaaa tgcccatttt gtcctgtacc agcatcaccg gtgccagggc
     2581 cagtaaaatg gccatgataa ttcccggaac gaccaccagc atgatgccaa tctgtactaa
     2641 aagggtagtc agaaaaatca gaataaataa cttgggcaat atcggcgcac tggcaccaat
     2701 agctcgcagc gcactgactc tctgacccgc agacaccagc tggataatta atattacgcc
     2761 tccggcgaga atggcgttac cgattaattc tgaaaaagtg gacgccgctg aagcctgcag
     2821 caaaatttgt tgctgttccg gtgacatatt ctgaaccagg tcgaacaacc cactactgcc
     2881 gctaacgggc acgccgtcat tgagctgcgc aagctgtgca tcactgggtg agaaaacatg
     2941 ccctaacacc actgtgataa acgcacatag caacgatacc aacagaatgg tcataaattg
     3001 attacggaag aaatttccgg tgtcacggta tacggactgc gccgtgatag acatgcactc
     3061 tccttgagtt ttatgcaggt gttaattagc gggcaattgt accctggtta agcctcgggt
     3121 ggcagcatca ggtcttgtat ggaaaagcat atctttgtaa agcggaggta attgctggcc
     3181 ttcaggcgtg ggcaaagcgc ggcaagtggc gatcgagtta atatttgcgt agagaaaata
     3241 tttaaaaatt gatttaaatc acattaacca ggattctcaa tgcaacttct aaattaatcc
     3301 agatcaataa atggtgaatt atcatatgta atgtgatcta tgtaggatca tttgttactc
     3361 caatgtaggt atattcgtca cgtttttata accataacga cggagcggat atgaaaaagt
     3421 taacagtggc ggctttggca gtaacaactc ttctttctgg cagtgccttt gcgcatgaag
     3481 caggcgaatt ttttatgcgt gcaggttctg caaccgtacg tccaacagaa ggtgctggtg
     3541 gtacgttagg aagtctgggt ggattcagcg tgaccaataa cactcaactg ggcttgacgt
     3601 ttacttatat ggcgaccgac aacattggtg tggaattact ggcagcgacg ccgttccgcc
     3661 ataaaatcgg cacccgggcg accggcgata ttgcaaccgt tcaccatctg ccgccaacac
     3721 tgatggcgca gtggtatttt ggtgatgcca gcagcaaatt ccgtccttac gttggggccg
     3781 gtattaacta caccaccttc tttgataatg gatttaacga tcatggcaaa gaggcggggc
     3841 tttccgatct cagtctgaaa gattcctggg gagctgccgg gcaggtgggg gttgattatc
     3901 tgattaaccg tgactggttg gttaacatgt cagtgtggta catggatatc gataccaccg
     3961 ccaattataa gctaggcggt gcacagcaac acgatagcgt acgcctcgat ccgtgggtgt
     4021 ttatgttctc agcaggatat cgtttttaat tccgcacaaa aacgaccccg taatatacgg
     4081 ggtcaataag gacatggtat aaagggtatt atttcttcgc ttctacgcca tcagtttcag
     4141 aacgaattaa aaatttctca gttgtttgcg gaatatgctg aatcagccag tcggccattt
     4201 gcttttcctc atttaaaatc gcttcgatga tcggaattga gactgtatca ccggcatttt
     4261 ttgctgctgc taatagtgag gtgtaacagg tgatttcaaa ttgctcgaag acatatccgc
     4321 caatagagcc tttaactatt tcatcagaag ggaatatacc accgattgac tgcccaagcg
     4381 cagccatttt actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa
     4441 tagtttccag ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc
     4501 gtagttcagg ataattatct atacggctgg ccatggattc aagcatagat tcggcttgct
     4561 tttccattgc gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa
     4621 tgtcctcttt ataaaatgac taaaagttaa aattcatatt tcaggcttta ttttcggctt
     4681 tcttatttac gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt
     4741 ccagggtttc tttcagaagc ttcgctgctt tacggtaacc taattgttca gccagcgtcg
     4801 ctaatgtccc gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg
     4861 cggcatcacg cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa
     4921 gaccttccat tgccacacat ttcatgcgct taattttcag atttgattct gattccacaa
     4981 cttgatcaat tcgttcaatc tgtccatgag tttcctcgag gtgcgcatga aaagcctgac
     5041 ttaatttttc atttgatgtt gctcttgcga gttttgccag tgcccgggtt aattgttttt
     5101 ctgcgctgta ggtatctgaa agcagttgaa taaatacatc ttcaatggtc ttcatattca
     5161 tatttttctc cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc
     5221 agctgatatt aataattaac ggtgaatcag gatttgcctg atttattacc accgctttgt
     5281 tgaccgcctt ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg
     5341 ctatgctgac cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt
     5401 cctgaactac cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta
     5461 atatgagtgt tgtgtgtcga cactcattaa aattagtcac taatgagaat tagtcaaatt
     5521 aagcgcaacg agaagataga gggaaatata ttttgaggaa cattctggat atattaacaa
     5581 ttacctgagg aatatgtgac ttagagaaaa ttattgattt tactggtgtt atgttgcggg
     5641 attaatttgt ttgattatat tactgttggg cggaaaaatg acgtaagttg acgttcgacc
     5701 ggggtaagcg aaacggtaaa aagataaata ttaaatgaat ttaggatttt tccggcttca
     5761 ttaaagaaag ttaaaatgcc gccagcggaa ctggcggctg tgggattaac tgcgcgtcgc
     5821 cgctttcatc ggttgtacaa aaactttcag tgccgccagc attttctctg gctcattaat
     5881 atgttgctcg atgattttaa caatggccga accagaaatc gcgcccgcag ctcctgcatc
     5941 aatcgctgct tttacctgat ccggggcgga aataccaaac ccctgcaatg gaggtgcagc
     6001 gttgtactct ttcagcttcg caaccagatg attgaggggt aacgcggcgc ggttttctgc
     6061 gccggtcacg cccgctcgtg acagcaaata tgtgtaacca cgaccgtaag aggctatctg
     6121 gcgcagcagg tcgtcatcgg catttggcgg gcagatgaag ataggtgcga cattatggcg
     6181 caacgcggcc tggcggaagg gcgcggactc ttcaactggc acatcggcaa ccagcaccga
     6241 atcgacgccg actttctcgc actgggcata aaactcatca atgcctttgt taaacaccag
     6301 attggcatac atcaacaggc caatgggaat ggtcgggtgt ttctggcgaa tcagtgccag
     6361 catttcaaaa cattgtgccg gagtcacacc tgccgcaaag gcgcgcagag tggcgttttg
     6421 aatggtcggg ccatccgcca gtgggtcgga gaaggggata cctaactcca gcgcgtcagc
     6481 accggcttca attagcgtat cgataatttt caatgactgc tcaatgcccg gatcaccgag
     6541 cgtgacgaaa ggaacgaatg cgccttcttt gcgctccttc aactgggcaa acagagattc
     6601 gtagcgttcc atcagatttc ccctcgtgct ttcaaaatat cgtgaacggt gaagatgtct
     6661 ttatcgccgc gaccggaaag gttaaccacc agtagctgct ctttatccgg gttttcgcgc
     6721 atcattttca gcgcatgggc cagggcgtgg gaggattcca gcgccgggat gatcccttcg
     6781 tgcaggcaca gcgttttgaa ggcttcaagg gcttcatcat cggtaataga cacgtaatca
     6841 gcgcgtccag tgctgttaag atacgcgtgt tgtggaccga cagacgggaa atccagtccg
     6901 gcggagatgg agtaagattc ttcaatctgc ccgtcttcgg tttgcatcat cggcgctttc
     6961 ataccgaaat aaatgcccac gcgaccatgt tttagcggtg cgccgtgctc gccagtttcg
     7021 ataccgtgac caccaggctc cacaccaatc aggccgacgt tggtttcatt gatgaaatca
     7081 gcaaacatgc cgatggcatt cgaaccgccg ccaacacagg cgataacggc atccggcagg
     7141 cgaccttctc tttccagaat ctgcgctttg gtttcttcgc caatcatccg ctgaaactca
     7201 cggacaatgg tcggataagg atgcgggcca gccgcggtgc ccagcatata gtgcgcggtt
     7261 tcataactgc cggaccagtc gcgtagcgcc tcattacagg catctttcag cgtggcggaa
     7321 ccgctatgta ccgggatcac ttccgcaccc attaagcgca tacgaaaaac gttaggcgac
     7381 tggcgttcaa cgtctttggc acccatataa atacggcatt tcaggccgag cagggcgctg
     7441 gcaagggccg acgccacgcc atgctgaccg gccccggttt cggcgatgat ttcggtttta
     7501 cccatccgct tcgccagcaa cgcctgcccc agcacctggt tggttttatg tgcgccgccg
     7561 tgcagcaaat cttcacgctt gagatacagc gtggtgttcg tcccggctgt aatgttctgg
     7621 catttggtca gtgcggttgg acgcccggca tagtttttca gcaggtcgtt gaactgggct
     7681 tgaaattcag gatctttttg cgcgctgaca aaagcttctt ccagctggcg cagagcaggc
     7741 atcaggattt gtggcacgta catgccgcca aactcaccaa aataggggtt aagtaatgtt
     7801 gtcattgttc ctttccttaa tatgcgcgca gcgtctggaa aaccgaggcc aaaagacgtg
     7861 cgtctttgat gcccggttgc gactctacag cagaattaaa atcaagtccg gcgcagccgg
     7921 tttgtgccgc ttccacgcag ttatctgcgc ctaagccccc cgccagcaga acgttgccaa
     7981 gcgattgacc atttaatagt gaccagtcaa aacgttgccc gcttccaccc tggccgttgt
     8041 ctaaaacata tttatcaacg tgctgaaact cgcgggcggg cagggtttca ccgacgctta
     8101 atgctttcca gatggcaaca tgtgctggca gagcttcacg cagcgtatcg atatacagct
     8161 gatcttcatt gccatgcagt tgcactgccg ccagcgataa caccttagct ttgtccacca
     8221 catcggcaat atcgtgattg cggaacacgc caacatactg caacggtgcc gcagccatca
     8281 cttcctgcgc ctgttcaacg ttgacgcaac gcggtgatgt cgcaacaaaa atcaacccac
     8341 cgtaaatcgc gcccgcgtca taagctgctt tagcatcttg cccacgcgtc aggccacata
     8401 ctttattctc acccagcaac acccggcgca cggcggcgtg caaatcgtca tgggccatca
     8461 acgccgaacc aatcagaaaa ccgttagcga agtggcttaa ctcgcgcacc tgagcgtaag
     8521 tattgatgcc ggattcgctg attaccgtta cgttgtgccc cagtttcggc gcaagctcgc
     8581 gggtacggtt gagatcaatc gacaaatcac gcagatcgcg gttgttgatg ccaacgacct
     8641 ttgcccctaa tgcaatggcg cgctccagtt cctcttcatt actgacttcg gtcagcaccc
     8701 ccatctccag actgtgagcg acggctgcaa gctggcgata ttggtcgtca tccagtactg
     8761 aaagcattaa taagcaggca tcggcctggt aatagcgcgc cagatagatc tggtaagggt
     8821 cgataatgaa gtctttacat aaaatcggct gcggggcgat ttggctgacg atgaggagga
     8881 aatcaaagct cccctgaaaa tatttctcat cagtcagcac cgaaattgcc gaagcgtaat
     8941 gtttataaat ggcggcaatg cgtgccggat cgaaatcatc acggatcacg ccttttgacg
     9001 gcgacgcttt tttacactcc agaataaacg ccgtgcgtgc accctgtagc gcatcataaa
     9061 aatgtcgtgt gctcggctga acctcattct gaaaactggc cagcggttgc tgctgtttgc
     9121 gggtttctac ccaaatcgcc ttgtctgcga cgattttcgc taaaacggtt tgcatcattt
     9181 accctcgtgc cgccagcgcg gtaactctgt cgtaagcgga accactgcgc agtacctcaa
     9241 gaacggtttg cgcattggct tgcagatctt catggccatg caggcgcatt aacatggcga
     9301 cgttcgcagc gacggctgct tcatgggcgg cgtcgccttt accttgtaac aagcgtgtta
     9361 aaatgtcacg gttttcttca ggtgttccgc ctgccagttg ctcctggtgg tagggagtca
     9421 ggccaaaatc ttcagcggtc aattgatagc tcttaatttt gccgtcatgc agctcggcaa
     9481 cgattgtcgg cgcgtgtaat gaaacttcat ccatcccgcc gctgtgcacc accgccgcgc
     9541 gttgataccc cagcacgcgc aaggtttcgg caatcggcag caccagttcc ggactataaa
     9601 caccaattaa cgccagcggc ggatgcgccg ggttaatcaa tggccccagc acattgaaca
     9661 gggtgcgggt tttcagttgc tggcgaaccg gcatcgcgtg gcggaatccg gtgtgatatt
     9721 tcggcgcaaa gaggaaacat acgcctaact cgtccagcgc ctggcgcgat ttattggcgt
     9781 tcatatcaag attaataccg aatgccgcca gcagatccga cgagccggat ttactggaga
     9841 cgctgcggtt accgtgtttc gccactttca gcccacaggc cgcggcgaca aacgcactgg
     9901 cggtagaaat attgatactg ttgctgccgt caccgccagt accgacgata tcagcaaaca
     9961 gataatccgg gcgcgggaac ggcgctgcgt tttccagtag cgcggttgct gccccggcga
    10021 tctcgttcgg gtgctcaccg cgaattttca tgctcaccag cgccgccgcc agttgttccg
    10081 gcttcagctc gccacgcacc accgctgaaa acagctggtg gctttcttgt tggctaagcg
    10141 tctgcgcctg atacagtttt tccagaatcg gttgcagcgt gttggctggc tctagtttct
    10201 gctgcgccca ggccagcgtt tgttccagca ggcgagcgcc ctgggtggtg agaatggatt
    10261 ccggatggaa ctggaatcca caaacgcgat ccgcatcgtg acgtactgcc atcaccatgc
    10321 cattaaaatg ggcgttgatg gttaaaccgg ccggaatgtt actgccaacc agcgagtgat
    10381 aacgcgccac cggcagcggg tttgttaatc cggcaaacat cgcctgaccg tcatgttcaa
    10441 tgctcgacgc tttaccgtga agaatttcgc ccgcctgacc gacatagccc ccgtaagctt
    10501 cgacaattgc ctgatgtccg aggcaaatgc caataatggg cagcttgcca cgcaagcggg
    10561 tgaggagttc cggcatacaa ccggcttcgc tcggcacacc ggggccagga gaaagcatca
    10621 gtaccggatt gctcatcgtc gccaggcgtt caattaaggt ctgcgccgga atatggttgc
    10681 ggtaaatcac cacgttatga ccattgctgc gcaactgatc tgccaggttg tacgtaaaag
    10741 agtcgatatt atcgagcagc agaatgtcag ccatcagaaa gtctcctgtg catgatgcgc
    10801 ggtggcaata gcgcgcagta cagcgcgggc tttgttacgg gtttcgtcgg cttccgactg
    10861 cggaacagaa tcaaggacta caccagcacc cgcttgcacg gtggcgatac cgttttccac
    10921 cagcgccgag cggatcacaa tgcaggtgtc gagatcgcca tgcgcggtga aataacctac
    10981 cgcgccgccg tagctgccgc ggcgacgacc ttccgcctcg gcaattaact gcatagcgcg
    11041 tactttcggc gcaccgctta acgtccccat attcatacag gcgcgataag cgtgcagggc
    11101 gtcaagatcg tgacgcagtt cgccgactac gcgagagacg aggtgcatca cataggaata
    11161 acggtcaact ttggtgagat cggcgatgta gcggctgcca ggggtgcaaa tgcgtgccag
    11221 atcattacgg gcgagatcaa ccagcatcag atgttcagac agctctttat ggtcagtacg
    11281 catttccagt tcaatacggc tgtcgagatc tctgtccagt gaaccatcgg cgcgacgacc
    11341 gcgtgggcgt gttccggcaa tcgggtagat ctcaatctgg cggctggtgg catcatactt
    11401 gagcgagctt tccggcgacg cgccaaatag ggtgaaatca ttatcctgca taaaaaacat
    11461 gtacgggctg ggattactct ttttcagcac gtaataggcc gccagcggtg acgggcaggg
    11521 cagagagaaa cggcgagatg gcaccacctg gaaaatttct ccagcgcgaa tcgctttttg
    11581 caacaaacgc actacgccac cgaactcttc atcgctctga ttacattcac aacgcatatg
    11641 cggcacggaa accactggca gcggcggcgc ggcttcggtc agttgctgac gtagttcgtt
    11701 caggcgagca gtgagacgtt gtttttcttc ttcattcgga gcaaacaggc tggcctgaat
    11761 acgggtgctt tttttctgat ggtcaatcac catcagcgtt tcagcgagat aaaaacagaa
    11821 atcagggcag ttattttccg ctgacagttg cggtaaatct tcaaatcccg ccacaaggtc
    11881 ataagagaac aggccgccga agaacattgc ttctcgttct tccttcggta cattcaacag
    11941 attctgcaat aaacggaaag cgtcaaaaac cgaaagggag cataagcggg cgtcttcatc
    12001 cagcagtgga ctgacagggg ggaagcgcag cacacggcag tttggtgatt gttcactttc
    12061 cacacccgca ggcagggcgt tatccagtag tgccagcagg gcttcgccat tgccggaaag
    12121 tgcctgaatt gtgacggtgt cacctaatgc tgtaatgcgc agcgcactgt ctatcagcag
    12181 caggcttttt aaatcatctt tgctgtcgat atctgcggat tccagcagca gcgttgccgg
    12241 acgattccca cacaactggt gaaaaagcgc agtcgggttg tcgcgataag cgccttcgca
    12301 ggttagcagt tcgagagtcg gtttttgtgt ttgcattgtt attctctaat tttgttcaaa
    12361 aaaaagcccg ctcattaggc gggctgggta tctgattgct ttacgcatgg tgaatacact
    12421 gcccgtttca ggaagtgcgc caccaacctt tcagtacgaa aattgctttc attgtcgata
    12481 ccctttttac gtgaacttgc gtactagtta actggttcga tgattaattg tcaacagctc
    12541 atttcagaat atttgccaga cccgttatga tgtcggcgca aaaaacatta tccagaacgg
    12601 gagtgcgcct tgagcgacac gaattatgca gtgatttacg acctgcacag ccataccaca
    12661 gcttccgatg gctgcctgac gccagaagca ttggtgcacc gtgcagtcga gatgcgcgtc
    12721 ggcaccctgg cgatcaccga ccatgacacc acagctgcaa tcgcgccagc aagagaggaa
    12781 atttcacgtt cgggactggc gctgaatctt attcccggcg tggaaatttc cacggtctgg
    12841 gaaaatcatg aaattcatat tgtcgggctg aatattgata ttactcatcc gctaatgtgt
    12901 gagttcctcg cgcagcagac agaacggcgc aatcagcggg cgcagcaaat tgccgaaaga
    12961 cttgaaaaag cgcaaatacc tggggcgctg gaaggcgcac aacgactggc gcagggcggc
    13021 gcagtgacgc gcggtcattt tgcgcgtttc cttgtggagt gcggcaaagc cagttcaatg
    13081 gcagatgtct ttaaaaagta tctggcgcgc gggaaaaccg gatacgttcc gccacagtgg
    13141 tgtacaatag aacaagctat tgatgtcatt catcattctg gcggtaaggc ggtgctagct
    13201 catcctgggc ggtacaatct tagtgctaaa tggctgaaaa gattggttgc gcattttgcc
    13261 gaacaccacg gcgacgcgat ggaagtcgcg cagtgccagc aatcgcccaa tgaacgcacc
    13321 cagctggcca cacttgcgcg tcagcatcat ttatgggcat cacaaggatc tgattttcat
    13381 cagccatgcc cgtggatcga actgggtcgt aaactctggc tgcccgcagg cgttgaaggc
    13441 gtctggcagc tatgggaaca gccgcagaac accacagaga gggaattatg agccagtttt
    13501 tttatattca tcctgataac ccgcagcaac gtctgatcaa ccaggcggtg gagatcgtgc
    13561 gtaaaggcgg ggtgattgtc tatccaactg attccggcta tgcgctcggc tgtaaaattg
    13621 aagataaaaa cgccatggag cgtatttgtc gtattcgcca gctgccggac ggtcacaact
    13681 ttaccctgat gtgtcgcgat ctttctgaac tctcgaccta ttcattcgtg gataacgttg
    13741 cgtttcgttt gatgaaaaac aacacgccgg gcaactatac cttcatcctg aaggggacga
    13801 aggaagtgcc acgccgtctg ttgcaggaga aacgcaaaac catcggtatg cgtgtgcctt
    13861 ctaacccgat tgcgcaggcg ttgcttgaag cactgggcga accgatgctt tccacttcgc
    13921 taatgctgcc aggcagcgaa tttaccgaat ccgatcccga agaaattaaa gaccgtctgg
    13981 aaaaacaggt agatttgatt attcacggcg gctatctcgg gcagaaacca acaacggtta
    14041 ttgatcttac cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat gtgaagcctt
    14101 tcttataagt gcgtaactct gtatactacg cggccttaaa atggcgctat acggcgccat
    14161 tgattctcct gttcgacgcc tgtgaaggcg acaccaaaag gaagctctat gagcgaaaaa
    14221 ttacagaaag tgctggcccg cgctggccac ggctcccgcc gtgaaatcga atctattatt
    14281 gaagccggtc gtgtgagtgt tgatggcaaa attgctaaac tcggcgatcg cgttgaagtt
    14341 acccctgggc tgaaaattcg tatcgatggt catctgattt cggtacgtga gtccgctgaa
    14401 caaatttgtc gcgttctggc ctattacaaa ccggaaggtg agttgtgtac ccgtaacgat
    14461 ccggaagggc gtccgacggt gtttgaccgt ctgccaaagc tgcgtggcgc acgctggatt
    14521 gccgtaggtc gtctggacgt taacacctgt ggtctgctgc tgttcaccac cgatggtgaa
    14581 ctggcaaacc gtttaatgca tccaagccgt gaagttgaac gtgaatacgc cgtgcgtgta
    14641 tttggtcagg ttgacgacgc gaaactgcgt gatttgagtc gtggcgtgca actggaagat
    14701 ggtccggcag cttttaaaac catcaagttc agcggcggcg aagggatcaa ccagtggtac
    14761 aacgtgactc tgaccgaagg ccgtaaccgt gaagttcgtc gtctgtggga agcggttggt
    14821 gtgcaggtga gccgcctgat ccgtgttcgt tacggtgata tcccactgcc aaaaggtctg
    14881 ccacgcggtg gctggacgga gctggacctc gcccagacta actatctgcg cgaactggtg
    14941 gagcttccgc cagaaaccag ctctaaagtc gctgtagaaa aagaccgtcg tcgcatgaag
    15001 gctaatcaga ttcgtcgtgc ggtgaaacgt cacagtcagg tgagcggcag tcgtcgttct
    15061 ggcgggcgta ataacggtta atcaaagtat gccggacgtc atatccggca tttttacaga
    15121 ttaataatca atccctattt gcgccttaac accggcatca aacgcatgtc tgatggggcg
    15181 cagctcactt accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat
    15241 aatcaccgtc tgttgatgtg gacgttcatt taacgcctgc accacttctt ccagtggcaa
    15301 atagtcatac gccaccatat acgtcagttc atcaagcaaa accatatcca gtgaggaatc
    15361 agcaagcatc cgctttgcat gttgccagac ttcgcggcag gcggcggtat cagactcgcg
    15421 gttttgtgta tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc
    15481 cagcagattg cgttcgccat taggccaggt gcctttaata aactgcacga cgcccacttt
    15541 ttttccgtga ccaactgcgc gtgttgccgt accaaatgcc gcggtggttt tgccttttcc
    15601 attgccggta aagacgatga taataccgcg ttcatcctgg gcctgggcca cacgagcatc
    15661 tactttttct ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt
    15721 cctggtttac ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg
    15781 cccatcagcc agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct
    15841 tcggtcggga aggcgcttgc gcgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg
    15901 acgcgcaggc gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat
    15961 ttcgacgctg catatgcacc ccagttggct cgtccctgac gtccaacgct tgatgaagta
    16021 aagaccagtg aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg ggtgagcata
    16081 aaggtggcat taacgttgat ctgcatgacg tcctgccaga cctgcggatt ttgttcgctc
    16141 attgggcaaa catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa
    16201 ttaacgacaa tgcgctgtgc cagttgttgg caattttcgg acgtgcaggt cagcaaatcg
    16261 agaataaacc actgtggctg acgcccagtt tcttcgttta tgtggctggc tacctgacgt
    16321 aatttttctt cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc
    16381 gcggcttcac gaccaatccc atcgctggct cccgtcacca ggataatgcg atcattgagt
    16441 aaatcttgtt ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc
    16501 cagtgtactt tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct
    16561 ggggcgataa caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc
    16621 gagacggctt aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg
    16681 gactcgtgga attgttgtct gaatatggtt tgtttttggc taaaatcgtt accgttgtgc
    16741 tagcgattgc ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc
    16801 gtggcgagtt acgggtcaac aatctcagcg aacagtataa ggagatgaaa gaagaactgg
    16861 ccgcggcgct gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc
    16921 acaagcaaga agcgaaagca gcaaaagcga aagccaaact gggcgaggtg gcaactgaca
    16981 gtaaacctcg cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact
    17041 cgctacgtga agagataacg gcggtactcg cagcattcaa accgcaggat caggttgtgc
    17101 tccgtctgga aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc
    17161 agcgtctgcg tgataaaaac attcctttaa ctgttacggt agacaaagtc gctgccagcg
    17221 gcggttacat gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg
    17281 gttccattgg ggtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata
    17341 ttgatatcga actgcacacc gccgggcagt ataagcgcac gctgaccttg ctgggtgaaa
    17401 ataccgaaga agggcgggag aaattccgcg aagagctgaa cgaaacgcat cagttattta
    17461 aagattttgt gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac
    17521 actggtacgg acaacaggcg gtagagaaag gcctggttga tgaaatcaac accagtgatg
    17581 aagttattct tagcctgatg gaaggccgtg aagtggttaa tgtacgctat atgcagcgta
    17641 aacgactcat tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgctac
    17701 gctggtggca gcggggtcaa aagccattga tgtaaaagac aaacgcgagg ctaagacctc
    17761 gcgttttgct ttaatcaacc agatgatatt tttctgaaag cacatgggcc aggtgtttga
    17821 acatattaaa caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt
    17881 cgccggtaaa taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga
    17941 gcgtatcctc atgctcacga atgattttgt tggcctcttt caacagcgtt tcgcggtcga
    18001 tgggttgtgt ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg
    18061 catggcttgc agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga
    18121 ttttgcatgc taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc
    18181 aatctggcaa tagatttgct tgacattcga ccaaaaatcc gtcgtgctat agcgcctgta
    18241 ggccaagacc tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaaggggtt
    18301 gatatccgca gagagcgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg
    18361 tgcgacgcat tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca
    18421 tcgttgagtc cccggcaaaa gccaaaacga tcaacaagta tctgggttgt gactacgtgg
    18481 tgaaatccag cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga
    18541 gtgccgactc tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc
    18601 tcgtcaaccg tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc
    18661 ctggtaaaga gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct
    18721 atctcgcaac cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga
    18781 ttggtggtga tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga
    18841 tccgccaggc atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg
    18901 cgcgtcgctt tatggaccgc gtggtggggt atatggtttc gccgctgctg tggaaaaaga
    18961 tcgctcgtgg tctgtctgcc ggtcgtgtgc agtcggtggc ggtccgcctg gtggtagagc
    19021 gtgagcgtga aattaaagcg ttcgtaccgg aagagttctg ggaagtcgat gccagcacga
    19081 ctacgccatc tggtgaagcg ttggagttgc aggtgactca tcagaatgac aaaccgttcc
    19141 gtccggtcaa caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca
    19201 gcgtgctgga acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct
    19261 ctacgctgca acaagctgcc agcacccgtc ttggttttgg cgtgaaaaaa accatgatga
    19321 tggcgcagcg tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc
    19381 tgagtcagga cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat
    19441 atctgccgga aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag
    19501 cgattcgtcc ttctgacgtc aatgtgatgg cggaatcgct gaaggatatg gaagcagatg
    19561 cgcagaaact gtaccagtta atctggcgtc agttcgttgc ctgccagatg accccagcga
    19621 aatatgactc cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc
    19681 gtattttgcg ctttgatggc tggacaaaag tgatgcctgc actgcgtaaa ggcgatgaag
    19741 atcgtatctt accggcagtc gataaaggcg atgctctgac gctcgttgaa ctaacaccag
    19801 cccagcactt taccaagccg ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg
    19861 aaaaacgtgg tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggatc
    19921 gtggctatgt gcgagtagaa aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca
    19981 ccgatcgcct tgaagaaaat ttccgcgagt taatgaacta cgactttacc gcgcagatgg
    20041 aaaacagcct cgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact
    20101 tcttctcgga tttcacccag cagttagata aagctgaaaa agatccggaa gagggtggta
    20161 tgcgcccgaa ccagatggtt ctgaccagca ttgactgccc gacttgtggt cgcaaaatgg
    20221 ggattcgcac agcgagtacc ggggtattcc ttggctgttc tggctatgcg ctgccgccga
    20281 aagagcgttg caaaaccacc attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg
    20341 aaggcgaaga tgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca
    20401 cggcgatgga cagctatctc atcgatccga aacgtaagtt gcatgtctgt ggtaataacc
    20461 caacctgcga cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc
    20521 cgatcgttga gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg cgtttcggta
    20581 aatacatggc ctgcaccaac gaagagtgta aaaacacacg taagatttta cgtaacggtg
    20641 aagtggcacc accgaaagaa gatccggtgc cattacctga gctgccgtgc gaaaaatcag
    20701 atgcttattt cgtgctgcgt gacggtgctg ccggtgtgtt cctggctgcc aacactttcc
    20761 cgaaatcgcg tgaaacgcgt gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc
    20821 tgccggaaaa actgcgttat ctggccgatg cgccacagca ggatccggaa ggtaataaga
    20881 ccatggttcg ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa
    20941 aggcgactgg ctggtcagca ttttatgttg atggcaaatg ggttgaagga aaaaaataac
    21001 ctttaattct gtcaggtttt tataaacaaa gggtcgcgaa agcggccctt ttttattgca
    21061 tattattttt tcttcacacc tatacactaa ggctataaat gatatagtgg ttatagttag
    21121 cacctttttt attattaaat cgtattagtc actcgccagg tgtgacgaaa aaacgatgtt
    21181 ctgatggcgt ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga
    21241 ggtggtcaat cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc
    21301 cgggatcagt aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg
    21361 aagcggcaag cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg
    21421 cgaagtcctg tcgaaagtcg atgccataaa atcggttgcc ggtgagcaca cctggccgga
    21481 taaaggctcg ctgtatatcg ccaccacgca tacccaggca cgctacgcgt taccaaacgt
    21541 tatcaaaggc tttattgagc gttatccccg cgtttctttg catatgcacc agggcccgcc
    21601 gacacaaatt gctgatgccg tctctaaagg caatgccgat ttcgctatcg ccacagaagc
    21661 gctgcatctg tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt
    21721 agtcactccg gatcacccgc tggcaggcaa aaaagccatt accattgaag aactggcgca
    21781 atatccgttg gtgacatata ccttcggctt taccggacgt tcagaactgg atactgcctt
    21841 taaccgcgca gggttaacgc cgcgtatcgt tttcacggca acggatgctg acgtcattaa
    21901 aacttacgtc cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt
    21961 cgccgatccc gaccttgtgc gcgttgatgc tcacgatatc ttcagccaca gtacaaccaa
    22021 aattggtttt cgccgtagta cttttttgcg cagttatatg tatgatttca ttcagcgttt
    22081 tgcaccgcat ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ccaatgaaga
    22141 aattgaggtc atgtttaaag atataaaact gccggaaaaa taatttcttg cgttattttc
    22201 ggcacctttt atgtagcgaa ggtgccggaa tatattctct tttgttactt atttctatac
    22261 cgtgcacaat atttttatcc ctcatattta tagggtaaat tacctgtaaa gtagtgtcta
    22321 attacccgcc tcgcaaattc cagttttcaa atatctattt caggtcaaaa gattgaatta
    22381 ttcactggag acgattcgta aattcgctgg atttttgact aaagtttctt taggatttgt
    22441 ctcatcgtat gattaattac accaatcatt caacgtcttg ctgataagtg atggcgatta
    22501 tatgaggtta gcaatgcctt ctggaaatca ggaaccccgc cgtgatcctg aattaaaacg
    22561 taaagcctgg ctggcggttt ttcttggttc tgcacttttc tgggtggttg tcgcactgct
    22621 gatttggaaa gtgtggggat aactatggtc ggtcaggagc aactggagtc gtcaccatta
    22681 tgccagcata gtgacaatga accagaaacg aaacgggaat gttccgtcgt tattccagac
    22741 gactggcaac taacatcgca gcagcaagcc tttatagaac tgtttgctga agatgatcag
    22801 ccgaaacaat aattatcatc attcttatta cccattttta atgaattaaa gggcttttaa
    22861 tacaccgcag caataacagc ttgagttatc tcaacacaaa ataataaccg ttaagggtgt
    22921 agcctatgat caacacaaat atgaaatatt ggtcctggat gggcgcgttt tctctgtcga
    22981 tgctcttctg ggccgaactc ctctggatca ttactcactg atccttgacc ccgctgcggc
    23041 ggggttgtca tttgctttgc cacaaggttt ctcctctttt atcaatttgg gttgttatca
    23101 aatcgttacg cgatgtttgt gttatcttta atattcaccc tgaagagaat cagggcttcg
    23161 caaccctgtc attaaggagg agctatgtcg tcaaccctac gagaagccag taaggacacg
    23221 ttgcaggcca aagataaaac ttaccactac tacagcctgc cgcttgctgc taaatcactg
    23281 ggcgatatca cccgtctacc caagtcactc aaagttttgc tcgaaaacct gctgcgctgg
    23341 caggatggta actcagttac cgaagaggat atccacgcac tggcgggatg gctgaaaaat
    23401 gcccatgctg accgtgaaat tgcctaccgc ccggcaaggg tgctgatgca ggactttacc
    23461 ggcgtacctg ccgttgttga tctggcggca atgcgcgaag cggttaaacg cctcggtggc
    23521 gatactgcaa aggttaaccc gctctcaccg gtcgacctgg tcattgacca ctcggtgacc
    23581 gtcgatcgtt ttggtgatga tgaggcattt gaagaaaacg tacgcctgga aatggagcgc
    23641 aaccacgaac gttatgtgtt cctgaaatgg ggaaagcaag cgttcagtcg gtttagcgtc
    23701 gtgccgccag gcacaggcat ttgccatcag gttaacctcg aatatctcgg caaagcagtg
    23761 tggagcgaat tgcaggacgg tgaatggatt gcttatccgg atacactcgt tggtactgac
    23821 tcgcacacca ctatgatcaa cggccttggc gtgctggggt ggggcgtggg tgggatcgaa
    23881 gcagaagccg ccatgttagg ccagccggtt tccatgctta tcccggacgt agtgggcttt
    23941 aaacttaccg gaaaattacg tgaaggtatt accgccacag acctggttct cactgttacc
    24001 caaatgctgc gcaaacatgg cgtggtgggg aaattcgtcg aattttatgg tgatggtctg
    24061 gattcactac cgttggcgga tcgcgccacc attgccaata tgtcgccaga atatggtgcc
    24121 acctgtggct tcttcccaat cgatgcagta accctcgatt acatgcgttt aagcgggcgc
    24181 agcgaagatc aggtcgagtt ggtcgaaaaa tatgccaaag cgcagggcat gtggcgtaac
    24241 ccgggcgatg aaccgatttt taccagtatg ttagaactgg atatgaatga cgttgaagcg
    24301 agcctggcag ggccaaaacg cccacaggat cgcgttgcac tgcccgatgt accaaaagca
    24361 tttgccgcca gtaacgaact gaaagtgaat gccacgcata aagatcgcct gccagtcgat
    24421 tatgttatga acggacatca gtatcagtta cctgatggcg ctgtggtcat tgctgcgata
    24481 acctcgtgca ccaacacctc taacccaagt gtgctgatgg ccgcaggctt gctggcgaaa
    24541 aaagccgtaa ctcttggcct caagcggcaa ccgtgggtca aagcgtcgtt ggcaccgggt
    24601 tcgaaagtcg tttctgatta tctggcaaaa gcgaaactga caccgtatct cgacgaactg
    24661 gggtttaacc ttgtgggata cggttgtacc acctgtattg gtaactctgg gccgctgccc
    24721 gatcctatcg aaacggcaat caaaaaaggc gatttaaccg tcggtgcggt gctgtccggc
    24781 aaccgtaact ttgaaggccg tatccatccg ctggttaaaa ctaactggct ggcctcgcca
    24841 ccgctggtgg ttgcctatgc gctggcggga aatatgaata tcaacctggc ttctgagcct
    24901 atcggccatg atcgcaaagg cgagccggtt tatctgaaag atatctggcc atcggcacaa
    24961 gaaattgccc gtgcggtaga acaagtctcc acagaaatgt tccgcaaaga gtacgcagaa
    25021 gtttttgaag gcacagcaga gtggaaggaa attaacgtca cacgatccga tacctacggt
    25081 tggcaggagg actcaaccta tattcgctta tcgcctttct ttgatgaaat gcaggcaaca
    25141 ccagcaccag tggaagatat tcacggtgcg cggatcctcg caatgctggg ggattcagtc
    25201 accactgacc atatctctcc ggcgggcagt attaagcccg acagcccagc gggtcgatat
    25261 ctacaaggtc ggggtgttga gcgaaaagac tttaactcct acggttcgcg gcgtggtaac
    25321 catgaagtga tgatgcgcgg caccttcgcc aatattcgca tccgtaatga aatggtgcct
    25381 ggcgttgaag gggggatgac gcggcattta cctgacagcg acgtagtctc tatttatgat
    25441 gctgcgatgc gctataagca ggagcaaacg ccgctggcgg tgattgccgg gaaagagtat
    25501 ggatcaggct caagccgtga ctgggcggca aaaggacctc gtctgcttgg aattcgtgtg
    25561 gtgattgccg aatcgtttga acgaattcac cgttcgaatt taattggcat gggcatcctg
    25621 ccgctggaat ttccgcaagg cgtgacgcgt aaaacgttag ggctaaccgg ggaagagaag
    25681 attgatattg gcgatctgca aaatctacaa cctggcgcga cggttccggt gacgcttacg
    25741 cgcgcggatg gtagccagga agtcgtaccc tgccgttgtc gtatcgacac cgcgacggag
    25801 ttgacctact accagaacga cggcattttg cattatgtca ttcgtaatat gttgaagtaa
    25861 caagtatttg cttgccggtt attttgcttc cggcaagcaa atgaattaca cgatgcaaga
    25921 gggttatttg ttcagcaaat ggcccatttt ctcggctttg gtatcgagat aatgttcgtt
    25981 attggggtta cgacctacaa tcaatggtac gcgttcaaca atattaatcc ctgcttcggt
    26041 cagaatttcg acttttttcg ggttattggt taacaagcgg acttcattga cgccaaggag
    26101 tttgaacata tcagcgcaaa gagtgaagtc gcgctcatca gcggcgaagc ctaactggtg
    26161 gttagcctct acggtatcgt aaccttgatc ctgcagtgcg taagcgcgga ttttattcag
    26221 cagaccaatg ttacgacctt cctgacggtg atacagcaaa ataccacggc cttcctcggc
    26281 aatttgcgtc aatgccgctt cgagctggaa gccacaatcg cagcgcaagc tgaacagggc
    26341 gtcaccggtc agacattcgg aatggacgcg cgcaagtacc ggggtatgcc cggaaatatc
    26401 gccatagact agcgcaacat gatcgtgtcc ggttgccagt tcttcaaatc ccaccatcag
    26461 gaaatcgccc catggggttg gcagtttggc ttctgccaca cgtttaagct gcatgaaatt
    26521 ctccagataa tgctggttct gtattggctt attttgccat aacgagaagg ggttcaccta
    26581 atcacgacgc gtcgatcgtt cacggaatgg cacaaatctg tcaatttttc ttggaactgg
    26641 cattttcagt tatgattgtg ggacttatca aaaaggagag gccatgcgtt cgattgcccg
    26701 acgtaccgca gtgggagctg cactattgct tgtcatgcca gtagccgtat ggatttctgg
    26761 ctggcgttgg caacctggag aacaaagttg gctactaaaa gcggcttttt gggttactga
    26821 aactgtcacc cagccctggg gcgtcattac acatttgatt ttgttcggct ggtttctctg
    26881 gtgtctgcgt tttcgcatta aggctgcctt tgtattattt gccattctgg cggcggcaat
    26941 ccttgttgga caaggcgtta aatcctggat caaagacaaa gtacaggaac cacgaccttt
    27001 tgttatctgg ttggaaaaaa cacatcatat tccggttgat gagttctaca ctttaaagcg
    27061 agcagaacgc ggaaatctag taaaagaaca gttggctgaa gagaaaaata ttccacaata
    27121 tttgcgttca cactggcaga aagagacggg gtttgccttt ccttccggtc acacgatgtt
    27181 tgctgccagt tgggcactgc tggccgttgg tttgctgtgg ccgcgtcggc gaacgttaac
    27241 cattgctatc ttgttggtct gggcaacggg agtcatggga agccgcctgc tgctcgggat
    27301 gcattggcca cgcgatctgg tggtagctac gttgatttcg tgggcgctgg tggcggtggc
    27361 aacgtggctt gcgcaacgaa tttgtgggcc attaacacca cctgcggaag aaaatcgcga
    27421 aattgcacaa cgagaacaag aaagttaacg ctggttgatt ttccgaattt aggccttaaa
    27481 tcatcaacaa tgcgcgtgga tgccatttcg cagacggcgc gaaaatggta ctttaaaggg
    27541 ctattgcggt aagttgacca taatttattc gctctaacca cataacggga agtaatgtga
    27601 aatatttact cattttctta ctggtgttag cgatcttcgt gatttcggtc acgttgggtg
    27661 cgcagaacga tcaacaggtg acgtttaatt atctgttagc gcaaggggag taccgtattt
    27721 ccacattgct ggcggtattg tttgctgcgg ggtttgctat cggttggttg atttgtggcc
    27781 tgttctggct gcgagttcgt gtttccctgg cgcgcgctga acgtaaaata aagcgactgg
    27841 aaaaccagct ttcacccgcg actgacgtgg ctgtagtgcc gcactcgtca gcggcgaagg
    27901 aataactttc tatgctggag ttgttgtttc tgcttttgcc tgtagccgct gcctatggct
    27961 ggtatatggg ccgcagaagt gcgcaacaaa acaagcaaga tgaagccaac cgcttgtcgc
    28021 gtgattacgt agcgggggtt aacttcctgc ttagtaatca acaggataaa gcggtagacc
    28081 tgtttctcga tatgcttaaa gaggatacag gcaccgttga agcccacctt acgctcggaa
    28141 acctgttccg ttcgcgtggc gaagttgatc gcgctattcg catccatcag accctaatgg
    28201 aaagcgcctc gctgacctat gaacagcgtc tgttggcgat tcaacaactg gggcgtgatt
    28261 acatggccgc cgggttatac gaccgcgcgg aagacatgtt caatcagctg accgatgaaa
    28321 ctgacttccg cattggcgcg ctgcaacagt tgctacaaat ctaccaggct accagtgagt
    28381 ggcagaaagc aattgatgtt gccgaacgcc tggtgaagct gggtaaagat aaacagcgcg
    28441 tcgaaattgc ccatttctac tgtgagttag ccctgcagca tatggccagc gacgatctcg
    28501 atcgtgccat gaccttgcta aaaaaagggg cggcggcaga taaaaacagc gcccgcgtat
    28561 ccatcatgat gggacgcgtg tttatggcga aaggagaata cgccaaagcc gtcgaaagtt
    28621 tgcaacgcgt catatcccag gacagagaac tggtcagcga aacgctggaa atgttgcaaa
    28681 cctgctatca gcagttgggt aaaactgccg aatgggcaga attcctgcaa cgcgcggtgg
    28741 aagagaacac cggtgccgat gctgaattga tgcttgctga tatcatcgaa gcgcgcgacg
    28801 gtagtgaggc tgcacaggtc tatattacac gccagcttca gcgtcatccg accatgcgtg
    28861 tattccataa gctaatggat taccacttaa atgaagcgga agaagggcgc gccaaagaga
    28921 gtctgatggt gctgcgtgac atggttggcg agaaggtgcg tagtaagcct cgttatagct
    28981 gccagaaatg tggttttacc gcatacaccc tctactggca ttgtccgtct tgtcgtgcct
    29041 ggtcaactat taaaccgatt cgcggtcttg atggcctgta atttttaaaa aaatccgact
    29101 ttagttacaa catactagtt attgatgtcc cattgtgctc cggcaacgac ggagcagaaa
    29161 aagcctgcta ggggaaaaat cgcaactgtt aattttttat tttcacgggt agaatgctcg
    29221 ccgtttacct gtttcgcgcc actcccggtg cccatcatca agaaggtctg gtcatgacgt
    29281 taactgcttc atcttcttcc cgcgccgtta cgaattctcc tgtggttgtt gcccttgatt
    29341 atcataattg tgatgacgcg ctgtcctttg tcgacaagat cgacccacgc gattgtcgtc
    29401 tgaaggtcgg taaagagatg tttacattgt ttgggccaca gtttgtgcgc gaacttcaac
    29461 agcgtggttt tgatatcttt cttgacctga aattccacga tatccccaac actgcagcgc
    29521 acgctgtcgc tgctgcagct gacttaggcg tgtggatggt gaatgttcat gcctccggtg
    29581 gggcgcgtat gatgaccgca gcgcgtgagg cactggttcc gtttggcaaa gatgcaccgc
    29641 ttttgattgc tgtgacagtg ttgaccagca tggaagccag cgacctggcc gatcttggcg
    29701 tgacactgtc acctgcagat tatgcagaac gtctggcagc actgacgcaa aaatgtgacc
    29761 ttgatggtgt ggtgtgttct gctcaggaag ctgtgcgctt taaacaggta ttcggtcagg
    29821 agttcaaact ggttacaccg ggcattcgtc cgcaggggag tgaagctggt gaccagcgcc
    29881 gcattatgac gccagaacag gcgttggcgg ctggtgttga ttatatggtg attggtcgcc
    29941 cggtaacgca atcggtagat ccagcgcaga cactgaaagc gatcaacgcc tctttacagc
    30001 ggagtgcatg atgagtgatt ccaacagccg tctggtctac tcaacggaaa ccgggcgtat
    30061 tgatgagccc aaagcggccc cagtacgccc taaaggcgat ggagcggtgc gtattcagcg
    30121 tcagaccagt ggacgtaaag gtaagggcgt ttgcctgatt accggtatcg atctcgatga
    30181 tgccgaactg acaaaacttg cagcggaact gaagaaaaaa tgcggctgcg gcggagcagt
    30241 taaagatgga gttattgaaa tccagggcga taagcgtgat ttattaaaat cactacttga
    30301 agcgaaaggg atgaaggtaa aactcgcagg cggttaacat aaaaagccac ggatatatcc
    30361 gtggctttcg aatattttac tgtgtgtatt attcagttat ttcgtactga acagaatcaa
    30421 gcgtattata attgcgagtc gataatcgac atttatttac gattatttac cgacctggtg
    30481 accaataaca ccaccgacag ctgcaccacc taatgtaccc aacgtactgc catcggtcag
    30541 tactgcaccg cctaatgccc ctgcacccgc gccgattgcg gtgttgcggt cccgtttaga
    30601 ccagttagaa caggcactca gagacattgc caaagtaatt gccagaacag ccgcggtcat
    30661 ttttttgctc gttacaaaca taatactctc tcctgaattt atgattcacg gaagtaagct
    30721 ctctataact ataatagcta agaataagcc tggtgaaatt attccgtgaa atctgctcgc
    30781 gcgcagggtt atatcacgca ggtgataatt acttcctgtt atatcgctga taataatttt
    30841 atatcttgag agtgttaata acaggtaaat agtcttaatt atcaaccagg aatcatctta
    30901 gagcggatga tttgccaaac tgcaaatcat ccgtagagaa gggaaatggt taaatgtcaa
    30961 cgacgtgaac ggtgagttta ctgtgctcaa gatgcatcag atcgctggcg cgtattttcg
    31021 aatcggtaat cacgcgattg aatcgctcaa cgggaccgat ggagtatgaa tgtacagcac
    31081 caaatttcga gctgtcagtc aggactatcg cttcgcactc tttttccagc acggcattga
    31141 ccacatcggt gcgcatcata tcgcgaccgg tgaatccagt ttcaggttgc cagccatcaa
    31201 taccaataaa tgctttgctg aaatgtacct gctggatgca ctggcgtgtc aaagggccaa
    31261 ccatactttc gctttttttc tggtacacgc caccgagcaa aatgacttca caaggcgcgt
    31321 ctttcagcaa atgcgcgatg tagctgctga ccgtgatgat agtgacattt ttcttctgct
    31381 cccccagagt ccgggcgagc agggcattgc tgctgccatt ttcgataaag atggtttcgc
    31441 ccggttgaac cagtgacgcg gcaaactcgg cgagttcacg cttcagcgta tagttgctca
    31501 tcatacgggt ttcgacgtca tcactatcaa gcgaaactgc aaagccatgt gcacggcgga
    31561 ggtaactcag tttttcgagg gtgttgagat cctggcgaat ggtaacttca gaaactccag
    31621 tggcttttgc cagatcggtt acgctaacct gaccctggtc aatgaccatc tgtagaatag
    31681 tttgttgtcg ggaattcatc gctgtaattc ttataacgtt ataatactaa ataaaaaaat
    31741 actgacgcgc cgggcatcct caaataggaa aacctgaagg cacgattgtt aaatctccca
    31801 cggtgtcttc gggatgtctg gcgtagattc agcaacctgg cgcaagagtt ctgctttaag
    31861 tatctcgaga ttatggatcg cagagtggta atcgcctgcc acaaggatat ctaacacaat
    31921 atcaatacgt tcggcaacgc gctgggcatc aaattcagac atcaaggaca tccttatagc
    31981 aaaaggggga aaatgaataa tgcaaaaatt gccgctaaaa gagaagtgtt taacagcaac
    32041 ggctaattat catccaggaa tacgatatat aatgacggga tatagcgcta agtatatata
    32101 ttcatctact tatgcgcgct tcagatagcg tttataccag cgttcgaagg cgacggcggg
    32161 catcggtttg gcaaacaaaa atccttgccg ctcattgatc ccgttcttgg ttaaaaaagc
    32221 atcttccttt gcactctcta caccttcggc gatcacctga agattcaatg cctgggccac
    32281 agcgacgatc gcccggacca gtgactgcga gacaggttgt ttgtgaatat ctcgaacaaa
    32341 aacctggtca agtttgatgg catcgatcgg aaagcgcgcc agttgcgaaa gtgaagagta
    32401 gccggtacca aaatcatcca gatgcacttg cgcacctagt tggctaaatt gttgaataac
    32461 agacagtgcc agttcatcat tctcaatcag acaactctct gtcagttcaa catctatagg
    32521 gcagtattca aaattgagtt cctggagaac ctgtttcagg gcggtgaaaa tggtttgatc
    32581 ggcgagctga cgtgcagaaa tatttaccgc cactcgcagg tttatgcctt tatcccgcca
    32641 ctttgccacc tggcgtacga catcgagaat cacccaacgg cctaaaggca caattagccc
    32701 tgactcttcg gcgtaggaaa tgaagtccaa cggtggaatc aacccacgtt caggtgactg
    32761 ccaacgtact agtgcttcca gactgcgcac ttcgacacgc taggtgattt tcgataacca
    32821 actgatcgtt ttccagtgct ttacgcaagt tggtatccag ccagagatat tcaaataccc
    32881 gctgattcat ttctggagta aaaacgcaaa attgtcctcg tccgccttcc ttcgctgtgt
    32941 acattgctgt gtcggcgtga cgaataatag tcgcgctgtc cgaaccatgt tcgggagaga
    33001 gcgaaatacc tactgaacag ctggtataaa cttcaattaa accaatacga aaggggaggc
    33061 gtaagcgggt caaaattcgt gatgccattg cttccagcgc gctttgtgag gtattggatg
    33121 ccagtaccag aaactcatcg ccacctggac gcgccaacac ctggtcatgt tcgagacagc
    33181 ttaaaatagc caatgacacg tcgcgtaata actggtcacc aaacaaatgc ccataggcgt
    33241 cgttgacctt tttaaaatta tccaaatcaa gatacacaac cccaacttta ttgttatctg
    33301 cctgattaat agcgtgatcg attaactcct gcattgcgtt acgattcggc agtccggtga
    33361 tactgtcggt atttgccaga atacgcagtc gctcctgagt gcggcgctct tcggtaatgt
    33421 cggtaccgga acagattaaa aaaatctcgt ttttgccact gccgctgtgg acaaatttat
    33481 tgcgaaacag aaacagccgc tggcctttac gtgttggtat ccacagttcg acttcatatg
    33541 cattgccgct tcgaaaaaat acacggttat tgcgcctgga tgccgcagct tcacgacggc
    33601 tcataaacag tttaaacacg ctttacccaa tgacgtcgtg ttctttcaac cctgtgtaat
    33661 cttcacataa ccgattgaag cgttggatat tcccccggct gtcgagaatc actattgcgg
    33721 aatgtgcttc agaaactacc tgctcagcaa aggaaagccc gtgtgataaa tcacgagcaa
    33781 ccgccggtgt gtcatgccat gcagacgcgt tgccagccca ttcccgttta ttgatttttc
    33841 gtcctaccag atgcacagaa agatcgcttt catcgacggt cagactcatc atcaggctgg
    33901 aggtgatgac cgtcatttcc ctgatgcgag cagcctgttc ggcagataac tgcaatgtac
    33961 gatcaggttc tgtggttttg gtggtagaaa agcgcaaagc atcgctgctt tccgtcaacc
    34021 gccagtatgg attgtgcgat ccgagaaagt tgtacaacgt tgtggactcc ctaacggttt
    34081 tcatgatgca tatcctccgg ttaacagcgg agtccgcggt taaataaaag gaacaacatg
    34141 acttacatga aattaacggc ggctaaacgc attgtttttt tatatattta tttgtaatcc
    34201 agttttggaa aaacgccagt tttcaaacga aagtcagtta aaaaatctgc ctggatataa
    34261 cgaaggtaga gcggggaaat aaacggccca tccatgagga atgggccgtg aaaggagatt
    34321 acgcgaccgg gcgcgcaata atgctgcggg tttccatgcg gacttcggca atggtgacgt
    34381 caataacgtc agttactttg taaaccgttt cacctttaat ttgtacagtg ccgttttcct
    34441 ggctgcaaac cagttcatcg cgcacagcgt gtaagaaagg tgccggaata aaggcgatag
    34501 cgccgttatc aaccaaacgc acacgcatgc cgccacggct gatatcgaca atttccgccg
    34561 cgaaacgggt gccggtcccg gctttatctt tcaggaagcg tgcgtataac cagtcgccaa
    34621 catcacgttc tgccatccgg ttgagacggc gacgttcggc catttggaca gtgatttcat
    34681 cctgcggacg cgtcgcagtt tcgcctttga taaccgcttt cagcagacgg tggttgatca
    34741 tgtcgccata tttacggatc ggcgaagtcc aggtggcgta tgcttccaga ccgaggccaa
    34801 agtgaggacc gggttcagag ctaatttcag caaatgactg gaagcgacga atgcggctgt
    34861 cgaggaaacc agttggttgc gcatccagtt cacgacgcag tttgcagaaa ccgtccagcg
    34921 tgagcacttc ttcggcatcg acatgcagac cgtgcgtttt cagcaacgct gccagcgcgt
    34981 cggcattcgc cggatcaaag cccatatgca cgttatagat gccaaaaccg agcttatcgc
    35041 gcagtacgcg ggccgcacaa atgttagcgg caatcatcgc ttcttcgacg atacggttgg
    35101 caatgcgacg aggctcggcg acgatatcca gcacttcacc tttttcaccg agaataaagc
    35161 ggtaatccgg gcgatcttta aacaccagtg cgtggttatg acgccactcg ccgcggcgtt
    35221 ggcaaatttg cgctagcaaa cggacttgtt cggcaattgc ttcactttca ggcttccagt
    35281 caccggtatt ttccagccag tcagaaacct ggtcatacac cagcttcgct ttggattcga
    35341 tggtggcggc aaagaattcg atattatctt caatggtgcc atcagcggag agcgtcatgc
    35401 ggcatgccag taccgggcgg acttcattgg cgcgcagtga gcagagatcg tcagaaagct
    35461 cgcgaggcag catagggatg ttgaagccag gcagatagtt ggtgaatgcg cgaattttcg
    35521 cggctttgtc cagcttgctg ccttcagcaa tccacgcggt tggatcggca atcgccacaa
    35581 tcagctgaag tttgtcatcc ggcagcgcct tagcgaaaag ggcgtcatcc atatcttcag
    35641 tgctggcact gtcgatggtg acaaaatcca gcgaggtcag atcttcacga accagacctt
    35701 catcgagcat ttcggtagcg acgccgtctg gtgcttcttt ttccagatta tggcgcgcaa
    35761 gggtaaccca ccacggtaca aagtggtcgt caccaaaagt gatgtattgt gtcagttcag
    35821 catagaaaga acgatcgcct ttcagcggat gacggcgcat ttcggcaacc gcccagtcgc
    35881 cttctttaaa ctcgtggttc aggccacggg ctgcgcggca aggaatggcg tcttttaaaa
    35941 gtggatgatc aggaacgatg gtcagacggt catttttgcc ctgaacttta cccacgaaac
    36001 gagtcaggaa cggttcaacc agttcttctg gctctgcgga ttcacgttct ttttcactgt
    36061 ggatcaccgc gataattcgg tcgccatgca tgactttttt catctgcggc ggcggaatga
    36121 aataactttt ttgtgcgtcg acttccagga agccaaagcc tttttctgtg gcttttacca
    36181 ccccttcagc gcgtggcgtc tgggaatgca gttgctgttt aagctgcgct agcagcgggt
    36241 tgtcctgaaa cataattgtc tattttggtg gccattagag cggctgacag ttttacgcga
    36301 atctgtctga cgcggcaagg ttaatatgtc tcacccaacg cgatttttaa gcgatttatc
    36361 cagccacaca gcccgctcca taccagcaga ttaataatct gcgttgatga tttttcgtgt
    36421 tcgagtaaag ggctgaactg ggcggcgcta aagcgatcgg gtgctcgggt cagccattgt
    36481 atcgcctgaa cggtagcgcg ctcaacagaa tgacggtcag cccatcggtt aatttcattt
    36541 tcgcccttgc gcagaagggt gctgattttt ttatattcat ttgatgaatc catacaggtg
    36601 gaaaaacaag caggactgcc atttatacgc gcgcataaca acgccgcaag ctgattatcc
    36661 ggctgtgcat tgacccgtgt gctgtttaac aacttttcga gaagggaaag tgaaataggg
    36721 tggagagaga gtgtttcggc cagttggcaa agttctgcag ttgaactttt tgtgtgctct
    36781 tcaggtagtt tttccacctc atagctgctt cgccagcgta ttgattcatc agcaaacagt
    36841 gacgcgtcgg cgtagttttg tatcgccagc ccgggtaacc agcgtaccgg atgcccgaga
    36901 taagcctgaa atgtcgcaat tgtccgcgcc tgaaagccaa taaaaccaat gatttggtta
    36961 atgatcacac agtcccagac agtaagaccg acgtcattca gatgctggcg cgatcggttg
    37021 tcgataatag aaggcgagct agccagttgg cgggcatatt gcgtgatttg tgccaaccga
    37081 tgattacttt ccctggagga atcaggtccg gggagcggcg taagtcgcgc cgaatagtag
    37141 ttacataacc gctggacgcc gcaaacctga gcaaccgtca gggccgtcga caagcgttca
    37201 taagcggtga aggtttgtga acgggtcacg gtaagctggt cagggaacaa ctccactgca
    37261 agaacgcgcg cagggaccag ccatgaaagg aagggggcca gcgtctcttc atcaaggatc
    37321 acgtcgagca gaaagggatc gctgaccttt gcggcttccg ggaccagagg cagaacgtca
    37381 tactccgtag tactggattg cgtttcatga taccagtggc ttttgccggt gatgtggcgt
    37441 tgttccatgg gcgctccttg gtcgtaaagg aaatcgttat cctgacgcaa ggcgggaagg
    37501 ggagaaagac ggatcggcga taacaaatat cagaaaggta taacagagat aacgggcggc
    37561 agaacgccgc ccatctttac caacagaacg attatttcag ttcgagttcg ttcattgcag
    37621 cgatgctgaa accgccgtca acgtggacta cttcaccgga gataccggca gagagatcgg
    37681 agcacaggaa tgccgcagag ttacccacat cttcaatagt aactgtacgg cgaatcgggg
    37741 taacggcttc gcaatgagcc agcattttgc ggaagtcttt gataccggag gccgccagag
    37801 tacggatcgg accagcagag atggcgttaa cacgcacacc ttccggaccc atcgcgttcg
    37861 ccatatagcg cacgttcgct tccagagacg cttttgccag acccataacg ttgtagttcg
    37921 ggatagcgcg ctcagcgcca aggtaggaaa gggtcagcag ggcagaaccc ggattcagca
    37981 tggagcggca agcttttgcc attgcaacga agctgtagga gctgatgtcg tgggcaattt
    38041 tgaagccttc acgggtaacg gcgttaacat agtcaccatc cagctgatcg ccaggtgcaa
    38101 aaccaataga gtgtacgaaa ccgtcaaatt tcggccaaac tttccccagt tcagcgaaca
    38161 tggtgtcgat gctggcatct tctgcaacat cgcactgcag aacgatgtca gaacccaatt
    38221 gagcggcaaa ttcttctacg cggcctttca gtttgtcgtt ctggtaggtg aatgccagtt
    38281 cagctccttc gcggtgcatc gcctgagcga taccgtaggc gatggacagt ttgctggcaa
    38341 caccggttac cagaatgcgc ttaccggaaa gaaaacccat agctttaatc cttattgttg
    38401 atgcttgttg tgcctgaaaa tcaggcgatt cgtcgtttta gtaaacagta cgaacagata
    38461 aacggttatt ataatcaacc tggctgtgag tagctatagt taccaggtcc gaccggagca
    38521 ggctgcggca gggggggcgc ttttcccctt accctaaccc tctccccaga ggggcgaggg
    38581 gaccatattg tgcaaatatt gttaccccag caacaaacag gctcatacag cccctaaccc
    38641 ttttatggcg atggctggga tggttcagac cttgccgaat attctccagc actgtctcca
    38701 tgttttcacc acaacagcta ttcggctcgg tcggccccct cgccctttca gggtgagggt
    38761 gaccggcggt tcagttcctg cagaataatc tccagcaccg cctcctcatt acaatcaatc
    38821 tcgttattcc agaaacgcag cacggtccag ccctgcgact caagccagct agtgcgcctg
    38881 gtatcatagg caactgccaa atcatgctgc ccaccatcca gctcaacgac tacgcgcgcc
    38941 gagcagcaag caaaatcgag aatgtagctc cccaccggat gttgacggcg aaatttgaaa
    39001 tcaccaaaac gtcggctgcg aagatatcgc cagagctttc gttcctgcaa agtgagattg
    39061 cgtcttaaat cacgcgcatt tgattttatt ttatccatca cctcattctg acaagattta
    39121 atctcttgtc agaatgaggt gaataatctg gaaggaggat tcagaaaatt agcgatcttt
    39181 acgccacgca tccgctgtca atgcttcgcc aaagtgcccg gcaatcagcc gtttggtgag
    39241 ttcatgcagc ggcgatgcca gcacatccgc ggtgctgcct cgctcgacaa cctcgccctg
    39301 atgcatcacc agcacctggt cgctaatgtg cttcatcatt ccgatatgct gggtaacata
    39361 aatatacgaa atgccctgtt tttcctgtaa ctccagcatc agattaatca actgcgaacg
    39421 cattgacata tccagtgaag cgagggcttc atcggcaata atgacttttg ggcgcaatat
    39481 cagcgcgcgc gccagaccca gacgctgttt ttgtccgggt gccaacatat gcggatagta
    39541 actgacgtga tccggtagca gcccaaccat acgcatcgtt tcaataatct gtttgcgacg
    39601 ctgttccggt tccaggtcgg tgttcaggcg cagtggaaaa tccagaattt gtgagatacg
    39661 ttgacgggga ttcaacgagg tcgaaggatc ctgaaaaatc atgcgaatac gctgactacg
    39721 gaaggaataa tcgccaaaat gcagcggatg atcgtcaatc aataactcgc cgctggtagg
    39781 ttctatcatt cccgccagca tttttgccag tgtggattta cccgaaccat tctcgccaat
    39841 aatcgccagt gtctggcctt cacgtagcgt aaagctcaag ggttttaccg cttctacggt
    39901 ctgacgacga aaccaaccgg tccggtagcg gaacgtttta cttaaattac gcacttcaag
    39961 cagcgtttcg atcatctcac tctttctcca tgttcagcgg gaaatgacag gcatagagat
    40021 gatttttcgc ccccgtcaaa cgtggcgtca caatgcattc tcgttgtgca tacgggcaac
    40081 gtggccccag acgacaacca atcggtaact gttccagcaa tgggatagcg ccgggtagag
    40141 tattgaggcg acttttatgc ggcatcgcgc tgccgaagtc tggtatcgcg cggatcagcg
    40201 cctgggtata aggatgatgt ggcatcgtca ccaactcctt actcggcgcg gtttccactg
    40261 tttgaccgca gtaaagcacg ttaattttat ccgcccattg gctaagcatt tgtaagtcat
    40321 ggctgataag caaaatagtg gtattgctgt tttggttgag acgcgtcagc aggcgaaaga
    40381 tttgcgcctg ggttgttggc tccattgagt ttgtcggttc gtcagcaatc agcagacgcg
    40441 gttgattcgc cagtgcaatg gctatcatca ctttctgaca ttcaccttcg gtcaactcat
    40501 agggaaaact gcgcatcgca tctttgtgat ctttaatccc cacgcggtgc agcagttcaa
    40561 tggcacggcg tttgcgccag ccaaaacgct gccaccaacg gcctttatag gtccaggctg
    40621 ggatgttttg catcaactgg cgacccacac gttctgaagg gtcaagacac gactgcggtt
    40681 cctggaaaat catcgacacg ttatggccaa ccagtttgcg ccgttcgcgt gcggagagac
    40741 gcagcaaatc gatatcatca aaacgcatac ggtcagcagt aacacgccag ttatctttat
    40801 tcaccccaca aatcgctttc gcaatcaaac tcttgccgga accggattca ccaacaagac
    40861 cgcggatttc accttcggtt aaggtcatac ttacccggtc gacggcttta acccactcat
    40921 caccggtttt aaattcaatg gtcaggttac gaatatcgag taatggcatt attccacccc
    40981 cgcaataatc gcacgacgaa cgccatcgcc aaggaggtta accaacaaca cgctaatcat
    41041 aattgccgca cctggcagca tgacagtcca cggggcgaca taaatcagtt ccagcgcatc
    41101 accgagcatc gctccccatt caggcgaggg gagttgtgcg ccgagatcga gaaagcccag
    41161 cgcggcgata tcgagaattg ccatcgacag tgcgcgggtg atctcggtga ccagcccggc
    41221 ggtgatgttt ggcatcacag caaaccagag aatattcagc gttgatgcgc catccagacg
    41281 ggcggcgata acgtactctt tttccagttc gtcatgcacc atgctgtaaa tcgaacgtac
    41341 catacgcggc agcaatgcca gccagacagc aaacatagcg tgggacaaac tcggtccggc
    41401 aaacgctaca acgataattg ccaacagcag cgaagggatc gccagtaagg tatccagaat
    41461 atggttaagc accgctgagc ggaggccgtg tgtcgccccg gcgaaggtgc ccagtaccag
    41521 gccacagatc gtcgcggcaa gcgtcaccac aaatgcgcca cccacggttg gcgcagctcc
    41581 gctcagtaaa cggcttaaaa catcacgtcc gaggtcgtca gtccccagga agaaagaaac
    41641 ttcgccatag cgcgaccatg atggcggcaa taattgataa ccgagaaatt gctggtcgat
    41701 gccgtaaggc gcaaaccagc cgccaaaaat acacagtaca gccagtcccg cgcagccgta
    41761 caggccgacc atcgcagagg cgtcactata aaatttgcgc caggcggtac gcagcgtgcc
    41821 cggcgggcgc ttttcgctgt atacgctatc gtaaggcata ccattcctta tgtttcagag
    41881 ggttagccat ggcacccaaa atatcagaaa tcacgttaac aataatgacc agtgagccac
    41941 acaccatcac tccggcggaa atggctgcgt aatcctgctg gcggatggcg ttaattaacc
    42001 agcgccctaa acccggccag ctaaaaacca tttcggtgat catcgccagc gtcagcatgg
    42061 tggaaaactg taagcccagg cgaggaataa ccggcggtaa cgcgttatgc agaacgtggc
    42121 gacgcaaaat agtaaagcgc gacaaaccgc gggtcgccgc cgcttttaca tagttctggt
    42181 catacacttc aatagtgctg atacgcatca ggcggatcac ttcagttgtt ggcgcaaccg
    42241 acagggtgat cacgggcaat atcatatggc ggattgcgct catgatcatt tcatcccgcc
    42301 atggtgagtc cgaaagccag gcatcaatca acgcaaaacc ggtaatcggt ttcacttcgt
    42361 agagcagatc gaaacgccct gaaacgggca accaacctag cgtgagtgaa caaaacagtg
    42421 tcaacagaag cgccagccag aacaccggga ttgaaaagcc cagtaaggcg atggcgttga
    42481 tcaagttatc ctgccactta tggcgcgtaa tcccggcaat catccccacg gggatgccaa
    42541 caatcagcgc aaaaccgaag gcgaggatgc acagttctat cgtcgccggg aagagctctt
    42601 ttaactgttc ggcaattggt tgaccgttaa tgctggaaac accaaaatcc cagtggatca
    42661 ggccattaaa ccagaacacc caggcattcc acagtgacgc gccttgtaac ggcgcgtgtg
    42721 gagtgaaata gctcaagctg aagccaacga aggtcagcag gaacaaggtg acaatcaata
    42781 acaaaatgcg gcgtaaggtg aagataatca tggttttttc acctcatcct gtttctcgcg
    42841 atacacccca gcaaaggagg cgttaccaaa cgggctaagt accagacctt tgatatcgta
    42901 ccgataggcc tgcaaacgca atgacgacgc cagcggcaga ataggtaatt cctgcgccag
    42961 aatactctgc gcttcgtcat aggcttcaat acgcgctgcc agctgctgcg aggagagcgc
    43021 cttacgcaac acgctgtcga atttcggatt gcaccagtgg gcgaggttgg tctgtgaatg
    43081 aattgccgcg cagctcagta acggacggaa gaaactgtcc gggtcgttac tgtccgtcgc
    43141 ccaaccggat aacgtcagat catggctcat atccatcaac cgcgcctcct gaaagcgacc
    43201 ttctaccggc acaatcacca cttttacgcc aacctgcgcc atatccgcct gaatcagttc
    43261 ggcagttttc agtggactgg ggttccacgc ctgcgaacgt gtgggcaccc acagtttcag
    43321 cgttaagttt tccagcccca acgccttcaa ctgttcgcgc gatttcgccg gattgtattc
    43381 agtaatttta gcctcgttgt cataggccca cgaggcgcgc ggtaaaatag aggccgccgt
    43441 ttcagccgta ccataataga tggattgcat caggcgctgg ttattaatcg ccagtgccag
    43501 tgcatggcgg acagcgggat tatttagcgg cggtttggcg gtgttaaatg ccagataggc
    43561 gacgttcatc ccaggacgca gcgttaaacg caagcgcggg tcgtcacgca aaatggatag
    43621 ctggctggca gcaggccagg ccagaacgtc gcattccccg gtcaggagtt tcgacagacg
    43681 tccggtgccg ccggagccta aatccaccac tacctgcggc attaacggtt taccgcgcca
    43741 gaagtcatca tgacgttgta ggcgaataaa ttgcccggcg cggtattccg acaactgata
    43801 cggaccggtg ccgaccggtt gacggtcgag ttgctcctgg cgatcttctt tctctaactt
    43861 ccgggcatat tctgccgaca tgaccgaagc ataatgggtt gcgaggtgcc acaaaaaaga
    43921 agcatccggc tgggcgagtc ggaactcaac ggtatgatta tccagtttgc ggacgctttt
    43981 cacgttatcg gcaaattgca ggctgtcgaa gtaggggaag ttgctgccgt tgacgttatg
    44041 ccacgggttg ttgcggtcaa aaattcgctg gaaggtaaac accacatcgt cggcattcat
    44101 tttacgagtg ggagtaaacc agtcggtttt ttgaaacgga acatcgcgac gcaggtggaa
    44161 gcgataggtc gcgccgttgt cgagtacttc ccagctttcg gcaagttccg gcatcaggcg
    44221 ataggtatag ggatcgacat ccagcagtcg atcataaaac tgggcggcaa gggtatcgac
    44281 aattaaccca ctgctcgctt tggatgggtt aaaggtgttg acttgcccgc tgacgcaata
    44341 gacaaaaccg ctgtcgcgga tatcagcatg cgggggggat tcaggcgcgg cgattgcctg
    44401 accactcaca agtccagcaa tcaccaaaag agacgataat acctggcgca taatattaag
    44461 ggattttatg taaagaggct atcttactaa tatttaatga catttgccat taccgtttgt
    44521 gttcaggggt cgtaatgagg gccgtgtggt tggtcgttga acaggtggcg tcgccatctg
    44581 ttcaacattc gtacccgtga tttctctata actataactc acagaacaac ttagcgagga
    44641 gcagagcggt acgatcgtga gaaacccatt aaagcacgct gcagtgcgtg tcattgttag
    44701 ccggatgcgg cgtgaacgct ttatccggac aacgatactg accgatcgtc tgcaattaaa
    44761 taattactca ttaccccatt gattcaaaaa ctctgcgatc tcatcaatgc gtacgggatt
    44821 aatccccgct tcagcagcca tttcatgttg ggcttcttcg ctgatctctt cattgttcat
    44881 caaacgggtg agcagtaact ggaagtagtg ggccagcggt gtgcgttcag cctctgcgac
    44941 aggctctgcg cattcggtcg cgtactcatc ggcaatatca aaatatttta gcgggatatc
    45001 gtggttcatt atttgcccct ggggttaatg cgctaagcat aagggcggat gatagcactc
    45061 gtgatccggg tcatacatct ttttatagtt aatggcgcgg cgcgttaccc tcaggcagga
    45121 taatgcgccg cgcatccggc tattacgttt cgctcaccgg cgttctgtca ccttcgtaca
    45181 gcggcacttt gcgatagcgg cggatcagat accacaaata tgcgccgcct aacgaagccc
    45241 ataccagacc gagtgtcagt gacgttgact ctaggttaac ccacagcaca cccaccgtca
    45301 gcgcgccaac cagcggcatc agcaaatagt ggaagtgatc cttccagctt ttattcattc
    45361 ctttacgccg ccagaaatga ttaaacaccg acagattcac gaaggtaaac gccaccagag
    45421 caccgaagtt aatcagtgct gtcgcggtga ctaaatcaaa gaacagcgcc gacagcgcga
    45481 caatcccgac cataatgacg ttgagtgccg gagtccgcca ttttgggtgc acatagccaa
    45541 acacgcgctc cggaaacaca ttgtcacgcc ccatcacata cagcagacgc gacacgctgg
    45601 catgtgaagc caggccagac gctaatgtgt tcacaaacgt ggtgcagagg aaaatcgact
    45661 gaaagagctt gccgccgacg tacagcgcaa tttcaggcag tgcggcgtcc gggtctttaa
    45721 agcggctgat atcgggaaag aacagctgca taaagaacga cgccgcgata aagataacgc
    45781 cgccgtatac cgccgtcagg aagatggctt tcgggatcac gcgtgcggcg tccggcgtct
    45841 cttccgaaag cgtggtcacc gcatcgaaac cgaggaacga gaaacagaca atcgtcgcac
    45901 cggtaataat cgggatcagg tgcgcgttct cgctgataaa cggctgaagt gaccagacgg
    45961 tgccaacgcc ttctccttta tgcagtccct gaaccaccag gaagataaac accaccatga
    46021 tggagatttg caccagtaca aacagggtat tgaagttagc gaccaggttg acgctcttca
    46081 gattcgcggc ggttaaaatg gcgacgaagg ttaccaccca cacccacggc ggcacttccg
    46141 ggaagagggc ggagagatag attttcgcca acaaaacgtt gatcattggc aaaaagagat
    46201 aatccagcag tgatgaccag ccgaccataa atccgacgtg cgggttaatc gacttttgcg
    46261 cgtaggtata ggccgaaccg gcctccggaa actggcgaac cagtttgccg tagctgatag
    46321 cggtaaacag cacgcccgcc agcgccagca aataggacgc cggaacgtga ccgtcgctaa
    46381 tgccggagac aataccaaaa gtatcaaata cggtcatcgg cgtgagatag gccagaccca
    46441 tcattaccac ctgccacaat ttcagtgatt ttcgcagacg ggttttgccg ggttgcgcag
    46501 caatattcag tggtgaatta atagccataa ttgctgcctc ccctgcgtaa taaggtttgc
    46561 ggtcgtgtcg acctgaaatc aaacctgttt agcaagggaa gcaaaggggg cggagtccag
    46621 tcagtagcag gaggggcgca tacgcagcct ccctcgccat gcggggattt aatcatcggc
    46681 atcatctcat ttcctcgtca cagtcttaat cgttaacgga agcccgttgc cgcctgcgct
    46741 ccataacgcg acatccggga aatgtctgga ttaccagcaa atgtcaggcc tggctccgct
    46801 caggccgatg aaacaacccc gcaaggggaa ttacgcgttt ttcaacatcc actcaatttc
    46861 tgtttctgtg atgaggcgct caaactgcaa cagctcatca tttttacagg cgtgatacac
    46921 atggcaaaac cgttcgccta aatagcggcg caggtgatca ttctcgataa actcacccag
    46981 ggcatcgctc tggcgaatcg ggaagggtaa gccttcctgt tccagcccgt tgccttcgac
    47041 ttcttcctgc aacggcagct cgttatcaag gccatgcaaa ataccggcaa aaatcgctgc
    47101 catcaccaga tatgggttgg catcggcacc cgccacgtga tattccacgc ggtgattatg
    47161 acggtcaccg cacggaatac gcagggcgac ggtgcggttg ttatggcccc acgacgcctg
    47221 cgtcggcaca tacattcccg gctggaagcg gcgatacgag ttcacgtttg gtgccagcaa
    47281 cgccatcgac gacggcatca ggtcaatcat cccggcgagc atctttttca gcagcggcga
    47341 atcttcgcct tctgtgtcag aaagcacatt ctcgccacga ttattttgca tactgatatg
    47401 gatatgcatc ccgctgcccg cgtgctcttc atacggcttc gccataaaag tggcgtgcat
    47461 cttatgcttt tctgccatca gacgcactag gcgttttagt gccagcgcat catcgcaggc
    47521 ttccagcacg ttatcagtat ggtaaaggtt gatttcaaac tgacccggcg aggcctcagc
    47581 gaccgcgcca tctgccggaa tcagctgcaa ctgcgccagt tcatcaatat cattgagcac
    47641 gtcggcgaag tggttgaggt tatcaacgga gtaaacctgg ctttgtgtat tgcggtcatc
    47701 ggtgccaggc gcgcagggcg gttgcagatc cccttcagcg tcgcgctggc gatccagtaa
    47761 atagaactcc agctctaccg ctacaaccgg gaacaatccg cgctggcgca gctgctgcca
    47821 caggcggtta agaacgttcc gcggctcaac gtcaaaggga gcgccatctt catcgaccat
    47881 ggtcaggagc atctgaccga taaactctgg atcggcggca gaaggagtta aggaaccgag
    47941 aacaggaaca caggtacgat ccggctcgcc catttcctga cccagacctg cttcttctac
    48001 tacgttgccc agaatatcca ttgcaaatac cgaggccggg aaataacacc ctttctcgag
    48061 cttcttcagg cttgaaacag ggatacgttt tccacggaag caaccattca aatcagtaag
    48121 taaaacatca acatattgcg tattcgggta gcgctccagg tagcgtttca cttcctgcgt
    48181 aaaggcgcta ccccgcctct cttctgactg ctgaacaaag ttctctactt caacgatatt
    48241 ggtttccatg attcttcgcc tttggtttgt tttccgctcg ttatcaaagc gtaaaatata
    48301 atgaccatca ttcgaatctg tatgcaaacc aaatgtttgt caaatgttaa attgagtttg
    48361 caaaaatgaa aatccactgc tagattgaaa aaatattgaa cataaaggtc atttaaagcg
    48421 cagtaatggc gataatttag tccattttgt gagattgagc atggaaaata taatgaacaa
    48481 tccggttatc ggtgtcgtaa tgtgcaggaa caggcttaag ggtcatgcga cccagactct
    48541 gcaagaaaag tacctgaatg ccatcatcca cgcaggcggc ttgccaattg cgctaccaca
    48601 tgcgctggcg gaaccgtcgt tacttgaaca acttttgccg aaactcgatg gcatttatct
    48661 tcctagtagt cccagcaatg tgcagccgca cctatatggt gaaaacggcg atgagcctga
    48721 cgccgatccc gggcgtgatc ttctgagcat ggcgataatt aatgccgcac tcgaaaggcg
    48781 catccccatt ttcgccatct gccggggttt acaagaactg gtggtggcaa ccggtggttc
    48841 gttgcatcgc aagctgtgcg aacagcctga attgctggaa catcgggaag atcccgaact
    48901 gccggtggaa cagcaatatg caccgtcgca tgaagttcag gttgaagagg ggggattact
    48961 gtctgcgttg ttacctgaat gtagcaactt ttgggtaaac tctctacatg gacaaggggc
    49021 gaaggtcgtt agcccacggt tgcgtgttga agctcgctcg ccggatggct tagttgaggc
    49081 ggttagcgtc atcaatcatc cttttgcgct gggcgtacag tggcacccgg aatggaacag
    49141 tagcgagtac gcgctttcgc gtatattgtt cgagggcttt atcaccgctt ggcagcacca
    49201 tatcgctgaa aaacagcgac tctgaccact acagtttaag gaaatgcaaa tatgagtgat
    49261 gagggactgg cgccaggaaa acgcttgtcg gaaatccgcc agcagcaggg gctttcacaa
    49321 cgtcgtgccg ccgaactctc cgggctgact cacagtgcta tcagtacgat agaacaagat
    49381 aaagtcagcc ctgccatcag tacgctgcaa aagctgctga aggtgtatgg tctgtcactc
    49441 tcggaattct tttccgagcc ggaaaaacct gatgagccgc aggtcgtcat taatcaggac
    49501 gacttaattg agatgggcag tcagggtgtg tcaatgaagc tggttcataa cggtaacccg
    49561 aatcgcacgc tggcgatgat ctttgaaacg taccagccgg gcacaaccac tggggaaaga
    49621 attaagcatc agggtgagga aataggcaca gtactggaag gtgaaattgt tctgacgatt
    49681 aatggtcagg attaccacct cgtcgcgggg caaagttatg ccattaatac cggcatcccg
    49741 cacagtttca gtaatacgtc ggcaggtatt tgccgaatta tcagcgccca tacgcccacc
    49801 acgttttaat ctttttgttc tgtaagccgg gtaagcgcaa tgcgcccggc aatccataaa
    49861 gaatgtcgcc agaaataaac gggcatgcag cccggggatc tctgcgccct gacgttcaca
    49921 aactgcatat atctgataga cgtgaaacag gagtcataat gaattttcat catctggctt
    49981 actggcagga taaagcgtta agtctcgcca ttgaaaaccg cttatttatt aacggtgaat
    50041 atactgctgc ggcggaaaat gaaacctttg aaaccgttga tccggtcacc caggcaccgc
    50101 tggcgaaaat tgcccgcggc aagagcgtcg atatcgaccg tgcggtgagc gcagcacgcg
    50161 gcgtatttga acgcggcgac tggtcactct cttctccggc taaacgtaaa gcggtactga
    50221 ataaactcgc cgatttaatg gaagcccacg ccgaagagct ggcactgctg gaaactctcg
    50281 acaccggcaa accgattcgt cacagtctgc gtgatgatat tcccggcgcg gcgcgcgcca
    50341 ttcgctggta cgccgaagcg atcgacaaag tgtatggcga agtggcgacc accagtagcc
    50401 atgagctggc gatgatcgtg cgtgaaccgg tcggcgtgat tgccgccatc gtgccgtgga
    50461 acttcccgct gttgctgact tgctggaaac tcggcccggc gctggcggcg ggaaacagcg
    50521 tgattctaaa accgtctgaa aaatcaccgc tcagtgcgat tcgtctcgcg gggctggcga
    50581 aagaagcagg cttgccggat ggtgtgttga acgtggtgac gggttttggt catgaagcgg
    50641 ggcaggcgct ttcgcgtcat aacgatatcg atgccatcgc ctttaccggc tccacgcgta
    50701 cgggtaaaca actgctgaaa gacgcgggtg atagcaacat gaaacgtgtc tggctggaag
    50761 cgggcggcaa aagcgccaac atcgttttcg ccgattgtcc ggatctgcaa aaagcggcaa
    50821 gcgccaccgc cgcaggcatc ttctacaacc agggccaggt gcgcatcgcc ggaacgcgtt
    50881 tgttactgga agagagcatc gccgatgaat tcttagccct gttaaaacag caggcgcaaa
    50941 actggcagcc gggccatcca cttgatcccg caaccaccat gggcacctta atcgacagcg
    51001 ctcacgctga ctcggtacac acctttattc gtgaaggcga aggcaagggg caactgctgc
    51061 tggatggtcg caacgccggg ctggcagcgg ccatcggccc gaccatcttc gtggatgtgg
    51121 acccgaatgc gtccttaagc cgcgaagaga ttttcggtcc ggtgctggtg gtcacccgtt
    51181 tcacatcaga agatcaggcg ctgcagcttg ccaacgacag ccagtacggc cttggcgcgg
    51241 cggtatggac gcgcgacctc tcccgcgcgc accgcatgag ccgacgcctg aaagccggtt
    51301 ccgtcttcgt caataactac aacgacggcg atatgaccgt gccgtttggc ggctataagc
    51361 agagcggcaa cggtcgcgac aaatccctgc atgcccttga aaaattcact gaactgaaaa
    51421 ccatctggat aagcctggag gcctgaaatg accgaacata ccagcagtta ctacgccgcc
    51481 agtgcgaata aatatgcacc attcgacaca ctgaatgagt caatcacctg cgacgtttgc
    51541 gtggttggcg gcggctatac cgggctctcc tccgcgctgc atctggcgga agcgggcttt
    51601 gacgtagtgg ttctcgaagc ctcacgcatc ggctttggcg caagcgggcg caatggcggg
    51661 cagcttgtga actcctacag ccgcgacatc gacgtgatcg aaaaaagcta cggcatggac
    51721 accgcccgta tgctcggcag catgatgttc gagggtggcg agatcatccg cgaacgtatc
    51781 aaacgttatc agattgactg cgactaccgc cccggcggtc tgtttgtggc gatgaatgat
    51841 aaacaactcg ccacactcga agagcagaaa gagaactggg tacgctacgg caataaacag
    51901 ctggagctgc tggacgtcaa cgctattcgc cgcgaggtcg ccagcgatcg ctacactggt
    51961 gcgctgctgg atcacagcgg tgggcatatt cacccgctaa acctcgccat tggtgaagcc
    52021 gatgccatcc gcctgaacgg cgggcgcgtg tatgagcttt ccgccgtgac gcagatccag
    52081 cacaccacgc cagccgtggt tcggactgcc aatggtcagg tgacggcgaa gtatgtgatt
    52141 gtcgccggga atgcgtatct gggcgataaa gtagagccgg aactagcgaa acgcagcatg
    52201 ccgtgcggca cgcaggtgat caccaccgaa cggctgtcgg aagatttagc ccgttcgctg
    52261 atcccgaaaa actactgtgt ggaagactgt aactatctgc ttgattacta ccgtcttacc
    52321 gccgacaacc gcctgctgta cggcgggggt gtagtgtatg gtgcacgcaa cccggatgac
    52381 gttgagcgtc ttgtggtacc gaaactacta aaaaccttcc ctcagttgaa gggcgtgaaa
    52441 attgattacc gctggacggg caacttcctg ctgaccttgt cgcacatgcc gcagttcggc
    52501 cgccttgata ccaacatcta ttacatgcag ggttacagcg gccacggcgt cacctgtacc
    52561 catcttgccg ggcgtttgat tgctgaactg ctgcgcggtg acgccgagcg tttcgatgcc
    52621 ttcgccaatc tgccgcatta cccgttcccc ggcgggcgca cgctgcgcgt gccatttacc
    52681 gcgatgggcg cggcttatta cagcctgcgc gatcgtctgg gcgtttaatt tccgattaac
    52741 agtgaagagt caaaaggtgt gaaacatgag caacaatgaa ttccatcagc gtcgtctttc
    52801 tgctacccca cgcggggttg gcgtgatgtg taacttcttc gcccagtcgg cagaaaacgc
    52861 tacgctgaag gacgtagagg gcaacgaata catcgatttc gccgcaggca ttgcggtgct
    52921 gaataccggg catcgccacc ctgatctggt cgcggcggtg gagcagcaat tgcaacagtt
    52981 tacccacacg gcgtatcaga ttgtgccgta cgaaagctac gtcacgctgg cggagaaaat
    53041 taacaccctt gccccagtga gcgggcaggc taaaaccgca ttcttcacta ccggcgcgga
    53101 agcggtggaa aacgcggtga aaattgctcg cgcccatacc ggacgacctg gcgtgattgc
    53161 gtttagcggt ggcttccacg gtcgtacata tatgaccatg gcgctgaccg gaaaagttgc
    53221 gccgtacaaa atcggcttcg gcccgttccc tggttcggtg tatcacgtac cttatccgtc
    53281 agatttacac ggcatttcaa cacaggactc cctcgacgcc atcgaacgct tgtttaaatc
    53341 agacatcgaa gcgaagcagg tcgcggccat tattttcgaa ccggtgcagg gcgagtgcgg
    53401 tttcaacgtt gcgccaaaag agctggttgc cgccattcgc cgcctgtgcg acgagcacgg
    53461 tattgtgatg attgccgatg aagtgcaaag cggctttgcg cgtaccggta aactgtttgc
    53521 catggatcat tacgccgata agccggactt aatgacgatg gcgaaa
//
LOCUS       DAJTYH010000013        52844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTYH010000013 DAJTYH010000000
VERSION     DAJTYH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            128..224
                     /gene="ffs"
                     /locus_tag="OO609_000792"
     ncRNA           128..224
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO609_000792"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            344..697
                     /locus_tag="OO609_000793"
     CDS             344..697
                     /locus_tag="OO609_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6632314.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(739..2289)
                     /gene="pdeB"
                     /locus_tag="OO609_000794"
     CDS             complement(739..2289)
                     /gene="pdeB"
                     /locus_tag="OO609_000794"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6632315.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2453..2923)
                     /locus_tag="OO609_000795"
     CDS             complement(2453..2923)
                     /locus_tag="OO609_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6632316.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3039..3590)
                     /gene="maa"
                     /locus_tag="OO609_000796"
                     /pseudo
     CDS             complement(3039..3590)
                     /gene="maa"
                     /locus_tag="OO609_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(3762..3980)
                     /gene="hha"
                     /locus_tag="OO609_000797"
     CDS             complement(3762..3980)
                     /gene="hha"
                     /locus_tag="OO609_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6632317.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4006..4380)
                     /gene="tomB"
                     /locus_tag="OO609_000798"
     CDS             complement(4006..4380)
                     /gene="tomB"
                     /locus_tag="OO609_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6632318.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(4926..8075)
                     /locus_tag="OO609_000799"
     CDS             complement(4926..8075)
                     /locus_tag="OO609_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6632319.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8098..9291)
                     /gene="acrA"
                     /locus_tag="OO609_000800"
     CDS             complement(8098..9291)
                     /gene="acrA"
                     /locus_tag="OO609_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6632320.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9433..10080
                     /gene="acrR"
                     /locus_tag="OO609_000801"
     CDS             9433..10080
                     /gene="acrR"
                     /locus_tag="OO609_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6632321.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10208..13570
                     /gene="mscK"
                     /locus_tag="OO609_000802"
     CDS             10208..13570
                     /gene="mscK"
                     /locus_tag="OO609_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6632322.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(13782..13943)
                     /gene="rsmS"
                     /locus_tag="OO609_000803"
     CDS             complement(13782..13943)
                     /gene="rsmS"
                     /locus_tag="OO609_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6632323.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(13957..14484)
                     /gene="priC"
                     /locus_tag="OO609_000804"
     CDS             complement(13957..14484)
                     /gene="priC"
                     /locus_tag="OO609_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6632324.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14554..14931
                     /locus_tag="OO609_000805"
     CDS             14554..14931
                     /locus_tag="OO609_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6632325.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15084..15635
                     /gene="apt"
                     /locus_tag="OO609_000806"
     CDS             15084..15635
                     /gene="apt"
                     /locus_tag="OO609_000806"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6632326.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            15764..17689
                     /gene="dnaX"
                     /locus_tag="OO609_000807"
     CDS             15764..17689
                     /gene="dnaX"
                     /locus_tag="OO609_000807"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6632327.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17022..17086
                     /gene="dnaX"
                     /locus_tag="OO609_000807"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            17742..18071
                     /locus_tag="OO609_000808"
     CDS             17742..18071
                     /locus_tag="OO609_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6632328.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18071..18676
                     /gene="recR"
                     /locus_tag="OO609_000809"
     CDS             18071..18676
                     /gene="recR"
                     /locus_tag="OO609_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6632329.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            18786..20660
                     /gene="htpG"
                     /locus_tag="OO609_000810"
     CDS             18786..20660
                     /gene="htpG"
                     /locus_tag="OO609_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6632330.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            20841..21485
                     /gene="adk"
                     /locus_tag="OO609_000811"
     CDS             20841..21485
                     /gene="adk"
                     /locus_tag="OO609_000811"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6632331.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21617..22579
                     /gene="hemH"
                     /locus_tag="OO609_000812"
     CDS             21617..22579
                     /gene="hemH"
                     /locus_tag="OO609_000812"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6632332.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22576..23535)
                     /gene="aes"
                     /locus_tag="OO609_000813"
                     /pseudo
     CDS             complement(22576..23535)
                     /gene="aes"
                     /locus_tag="OO609_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            23687..24991
                     /gene="gsk"
                     /locus_tag="OO609_000814"
     CDS             23687..24991
                     /gene="gsk"
                     /locus_tag="OO609_000814"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6632333.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25124..26800)
                     /locus_tag="OO609_000815"
     CDS             complement(25124..26800)
                     /locus_tag="OO609_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6632334.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27038..28258)
                     /gene="fsr"
                     /locus_tag="OO609_000816"
     CDS             complement(27038..28258)
                     /gene="fsr"
                     /locus_tag="OO609_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6632335.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28476..30128
                     /gene="ushA"
                     /locus_tag="OO609_000817"
     CDS             28476..30128
                     /gene="ushA"
                     /locus_tag="OO609_000817"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6632336.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30165..30644)
                     /gene="ybaK"
                     /locus_tag="OO609_000818"
     CDS             complement(30165..30644)
                     /gene="ybaK"
                     /locus_tag="OO609_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6632337.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(30848..31642)
                     /locus_tag="OO609_000819"
     CDS             complement(30848..31642)
                     /locus_tag="OO609_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6632338.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            31780..32121
                     /locus_tag="OO609_000820"
     CDS             31780..32121
                     /locus_tag="OO609_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6632339.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32336..34840)
                     /gene="copA"
                     /locus_tag="OO609_000821"
     CDS             complement(32336..34840)
                     /gene="copA"
                     /locus_tag="OO609_000821"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6632340.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35102..36034
                     /gene="glsA"
                     /locus_tag="OO609_000822"
     CDS             35102..36034
                     /gene="glsA"
                     /locus_tag="OO609_000822"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6632341.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36037..37329
                     /locus_tag="OO609_000823"
     CDS             36037..37329
                     /locus_tag="OO609_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6632342.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37454..37861
                     /gene="cueR"
                     /locus_tag="OO609_000824"
     CDS             37454..37861
                     /gene="cueR"
                     /locus_tag="OO609_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6632343.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(37862..38320)
                     /locus_tag="OO609_000825"
     CDS             complement(37862..38320)
                     /locus_tag="OO609_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6632344.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38317..39234)
                     /locus_tag="OO609_000826"
     CDS             complement(38317..39234)
                     /locus_tag="OO609_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6632345.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39380..40057
                     /gene="fetA"
                     /locus_tag="OO609_000827"
     CDS             39380..40057
                     /gene="fetA"
                     /locus_tag="OO609_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6632346.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40044..40823
                     /gene="fetB"
                     /locus_tag="OO609_000828"
     CDS             40044..40823
                     /gene="fetB"
                     /locus_tag="OO609_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6632347.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(40886..41740)
                     /gene="cnoX"
                     /locus_tag="OO609_000829"
     CDS             complement(40886..41740)
                     /gene="cnoX"
                     /locus_tag="OO609_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6632348.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(41801..42610)
                     /gene="ybbO"
                     /locus_tag="OO609_000830"
     CDS             complement(41801..42610)
                     /gene="ybbO"
                     /locus_tag="OO609_000830"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6632349.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42600..43222)
                     /gene="tesA"
                     /locus_tag="OO609_000831"
                     /pseudo
     CDS             complement(42600..43222)
                     /gene="tesA"
                     /locus_tag="OO609_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43193..43879
                     /locus_tag="OO609_000832"
     CDS             43193..43879
                     /locus_tag="OO609_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6632350.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            43876..46291
                     /locus_tag="OO609_000833"
                     /pseudo
     CDS             43876..46291
                     /locus_tag="OO609_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47004..48098)
                     /gene="mnmH"
                     /locus_tag="OO609_000834"
     CDS             complement(47004..48098)
                     /gene="mnmH"
                     /locus_tag="OO609_000834"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6632351.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48167..49093)
                     /gene="allS"
                     /locus_tag="OO609_000835"
     CDS             complement(48167..49093)
                     /gene="allS"
                     /locus_tag="OO609_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6632352.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49323..49805
                     /gene="allA"
                     /locus_tag="OO609_000836"
     CDS             49323..49805
                     /gene="allA"
                     /locus_tag="OO609_000836"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6632353.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            49883..50698
                     /gene="allR"
                     /locus_tag="OO609_000837"
     CDS             49883..50698
                     /gene="allR"
                     /locus_tag="OO609_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6632354.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            50788..52569
                     /gene="gcl"
                     /locus_tag="OO609_000838"
     CDS             50788..52569
                     /gene="gcl"
                     /locus_tag="OO609_000838"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6632355.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52582..>52844
                     /locus_tag="OO609_000839"
     CDS             52582..>52844
                     /locus_tag="OO609_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6632356.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVA"
BASE COUNT        12754 a        13586 c        13918 g        12586 t
ORIGIN      
        1 gaaaaacgcc cgagtgaagt cgcattgcgc aagaaaccag catctggcac gcaatgggtt
       61 gcaattagcc ggggcagcag tgataatgcg cctgcgcgtt ggttttcaac gctctcaatg
      121 ggggctctgt tggttctccc gcaatgctac tctgtttacc aggtcaggtc cggaaggaag
      181 cagccaaggc agatgacgcg tgtgccggga tgtagctggc agggccccca cccatttctg
      241 cctcccaccg tttcgtcaaa aaatcccagc atggctaaac tttaaccaca actgacgtcg
      301 caagaattgt ctggctgcgc agtacgcttc ggaggtatgt ctgatgaagt atgttgatgg
      361 ttttgtggtt gccgttcctg ccgataaaaa ggatgcctat cgggaaatgg ccgctaaggc
      421 cgcgccattg tttaaagagt ttggcgcgct tcgtattgtc gaatgctggg ccagcgatgt
      481 gccggatggc aaagtgaccg attttcgtat ggcggtgaaa gcggaagaga atgaagaggt
      541 tgtctttagc tggattgaat acccttcaaa agaggtccgc gacgcagcta atcaaaagat
      601 gatgtcggac ccacggatga aagagttcgg cgagtccatg ccgtttgacg gcaagcgaat
      661 gatctatggc ggattcgagt caatcatcga cgaataggta gcgtgacggg ctgtgcggcg
      721 tgccgtcagc ccaaaaattc acaaatgttg ctcggcccag cgtaagaaat cttctttcgg
      781 taatgccttg ctgtagagcc agccctgacc gtagtgcacg ccatgctggc gtagccactc
      841 ttcttgttta ctggtttcga ttccctccgc taccattttc agtttcagtg tttttgccat
      901 ttcgatgata tgcggcgtga catttttata ttccagcgca tcaacgaaag atttatcgat
      961 cttcagaatg tcgacatcca gatcctgtaa ataacttaaa cttgaatacc ccgtaccaaa
     1021 atcatcaaga taaatttcat ggcccgcctc ccggtagcga gaaattatcg gggcgctggt
     1081 tttcggatcg gcaaactcgc gttcagtgag ttcaagcgcg atctgtctgg gattaacctg
     1141 atagtgattg atcatttcac gcagcaattg cgggattttt tccgaggtga gcacggtgga
     1201 ttcaagattg atcgaaatat gctgctgtgg atgctgacgc aagcagtcgc ccatatcttc
     1261 aaagacgctt cttataatca gtagcgtcaa tggctcagaa aggcccgttt gctgtgccag
     1321 cggaataaaa ctatctggtg acaaccaact accgtctgtc tgcggccagc gcgccagtgc
     1381 ctcagcaccg acaattttgc cattggctaa ggagacaatc ggctgatagt gcacgcaaat
     1441 atcacgattt tcgatagcat cctgcagccg atgatgcggt gattggatac ggcgcaaaat
     1501 acgcagcaca aacatcgctg ccagcaggcc aatcaccaac ccgagcggta accaaataaa
     1561 gacttgccga tgccagcctt tctgcaacat tttcgttgaa gcccaggtga tgatcgaaat
     1621 attcatctcc aggaagggca gtatatcgta aatgatccca ttattttcga tatgctcacc
     1681 gggtgttttt tgtagcctgg taataattcc ctgagcaatt tcatcgctgc tggttatgac
     1741 aacgttatgg gcattgccaa taatggcggc atcaatttgc catgaactat agggaatgac
     1801 atcaataaag gaagcggggt cgatcatgac gacataatgt gccgttccca tggcgaccat
     1861 gtaacggata atgcctaaat cgttatgcga tgttaaccag acacgataac catctttcga
     1921 aattttacct agctcgggaa aggtatcggg cggactttca tgctccagag acgaacactg
     1981 gggaacgttg ttatcgatat aaaccacttc ctgaatatag cgataactgt aagatacccg
     2041 acgcatttcc atgagatggg cttcgctaca ggcagcgcct tgccatcttt ccagctcctg
     2101 cagcgcatct ttcccttgtg tcgccacctt attggctcga atagcgacgc gggaggaata
     2161 ggtatccagc tcttcaataa acgatgtttc tacctgctga tgggccagcc agatgcttaa
     2221 gccgacaggc agcaatactg aaagaatcag tactcccgaa ataaggccga ccagatgtcg
     2281 tgttctcacc agcatatcct tattaacaca gtgtagggaa agtatatctg attgtggaaa
     2341 aaggctggca ggagataagg aggtagagct ggtatttcat gaagtgatgg gtggaaacga
     2401 tcattgtcat ttgaccaggc gcagagcttt acgcctggta tgtaacccta acttaagatg
     2461 ttgattcggc gcgagcgagg gtaaaaatat cgtaaaaaga cagttcacct ttacggacga
     2521 tcattttttg cagttgttcc ttatgaacat ccgcgacacg cggagagttc aagatggctt
     2581 caaccagcgc ggcaggtaca aagcgcgtgt tgtaccattc gccgttatag caaacgcgga
     2641 aatccagcac gtcgatatct tcatagcggt agcgtgggta gacatcgaaa aagaagaaac
     2701 cattaagcag gataaataat acaaccagta gccagacaga gcctgacagc gtttcggact
     2761 gcaacatcac cgccagggtg gcaaaaaaag caacgtacat accgataaac agccccggat
     2821 gtttacggat aaaactgata ctaaagcggg gtttgttgtc gcgtttttcg cgggtattca
     2881 gagactcaat cgtttcggtc agcaggcgtt gtatttcggt catcgtttgc cttcgtggta
     2941 tctgcaactt tccagggaac accctatttt aacgtgggaa tagtagaggg gaagtaacag
     3001 atctaccaca attttgcata acagttgcga aaaaccgatt acaatttttt aattattctg
     3061 gctggattac cgcccacgac aacgttgtcc gggacacctt ttgtgacaac tgcacctgaa
     3121 gctaccacga cgttatcacc aatggtcaca ccagggttaa tgaccgcgcg tccgccaatc
     3181 cagacgttat taccgatggt gacgggtttc cccagttcag caccgctatt acgtgctaca
     3241 gggtcgatgg gatgtgttgc cgtgtagata tgaacgcctg gtgccaacat acagttatca
     3301 ccgatgcgaa tagggcagac atcaagcatc acgcaatcga agttggcgaa aaaattattg
     3361 ccgagaaaaa tgttatagcc ataatcacag cgaaacgttg gctcaatata agcctctgtc
     3421 acctgaccga ataaattagc gagaatttac tggcgtaatg tgtgctcttc cgccagggaa
     3481 tgattgtatc ggtgaataag ctgacgagcg cgcaggcgat cgcgagataa cgtctcatct
     3541 gccgagcgat acaactcacc agcaatcatc ttttcttttt ctgtgctcat tactgacctc
     3601 cggttgctga aaatgcggca acacagtaag acgaaaagtc agtgaagaga acgttctcat
     3661 tacatctgtg tgataaagat cacatagggt atgtcttcat ggcgaaaaag tataaaattc
     3721 ttaataaaca gccggttata gcaccgaaag cgaattattt attagcgaat aaatttccat
     3781 actgaggaag ggatcttgtc gtacagttta ttcatggtca attcggcgag gcggtgatct
     3841 gcggctgagt aaaataccgc cagttcatta tctgataatt cgtatttatt tttctcgata
     3901 acacgctcca gcgtgtcaat tgtctggcaa cgacgtaaac gcattaaata atcggttttt
     3961 gttaaaggtt tttcggacat acttctaccc atggttgtaa taattctaac aagataaact
     4021 cgcaggattc tctttcgtcg cgttgacaaa acaacggaat agtcgattac ctgacttccg
     4081 ccatttctga agagcctgca tattaatacc ataactgctg aacaacataa aggtgtcatc
     4141 cagatattcg tcgatctgct caatgagctt attgtcttca ttatacttaa ttttgtaatt
     4201 aagtgcgaag gtcgcaatat gctcaatcag ttcattcaac tggaggttga tcgccgaggt
     4261 tgggtcgtta acccaaccat gattgctttc ttcaaggttt gcaagacagt catgatacag
     4321 ggtttcacag agaaacttaa gctgtgcgat atcatgtctt ttgggtgagt attcatccat
     4381 aacgcgtccc cttcttagtg gttgaactaa cggacacctt tcgggatgga aaaaacttac
     4441 tgacctagac ttgccctctc gtttgttagc ttaactataa gccactcttt gcaggttttc
     4501 atcgcattat tacgaaacat tacaattagg aaggaatcgg gacaggactc actttttcat
     4561 actagcacta acccgcagca ggtaaaagca gtttgctcga actgttcata acatttcagg
     4621 attatcgcca gatgaaattg gtgacactaa taccaggatt gctctgaata tgacgtaata
     4681 accgaggaat gaataaagaa ttaccgcaaa taattaagaa tagcctctaa atgattatgg
     4741 attattaaat ctattagcgc tacttaatat aaattaacta acaatcagta ttttttatga
     4801 attttatacg tggttaatac tggttttcgt atgagatcct gagttggtgg ttcaattact
     4861 ccttaatgtt cgtaggttat gcataaaaaa ggccgcttac gcggccttag tgattacatg
     4921 ttgtatcaat gatgatcgac agtatggctg tgctcgatat cttcattctt gcggctaaag
     4981 cggcggcgaa ccaccacaaa gaataccgga acgaagaaga ttgccagtac cgttgcggtc
     5041 accatcccgc ccattacacc ggtacctact gcgttctgcg cgccggaacc agcaccagta
     5101 ctgataacca gcggcataac gccgaggata aacgccagcg aggtcatcag gatcggacgt
     5161 aaacgcatcc gcaccgcatc aagcgtcgct tcaatcagac ctttaccttc tttatccatc
     5221 aagtctttgg cgaattcgac gataagtatc gcgttcttcg ccgacaaccc aatggttgtg
     5281 agcagtccta cttggaagta aacgtcattg gtcaggccac ggaaggtggc agccagcaac
     5341 gcaccgataa cccccagcgg aacgaccagc ataacggaga acggagtcga ccagctctcg
     5401 tacagcgccg ccagacacag gaatacgaca atcaacgaaa tcgcgtacag tgaaggtgcc
     5461 tggttgccgg agagacgttc ctgataggac atccccgtcc agtcatagcc aacaccggta
     5521 ggcagtttgc tcgccagttg ttccatcagc tccattgctt caccggtact tttacccggt
     5581 gccgcctggc ctaagatttc catggatggc aggccgttgt aacgttccag acgcggcgaa
     5641 ccgtactccc aacgagaaga ggagaacgcc gagaatggca ccatctgacc atcagcagca
     5701 cgaacatacc agtcgccgat atcatccggc agcatacggt atttcgcttc tgacatgacg
     5761 taaactttct tcacacgacc gcggtcgata aagtcgttca catagctacc gccccatgca
     5821 gcgcccagag tggtgttaat gtcgttgata gaaacaccca gcgcctgcgc tttttcctgg
     5881 tcgatatcaa tcttaaactg cggggtatct tccagaccgt ttggacgtac gctggtcagc
     5941 atatcagggt gcttcgctgc ttctgcaagc agctggttac gcgcctgagt cagtttttcg
     6001 tgaccaaggc cagcctggtc aatcagctca aagtcaaagc cggttgcagt acccagttcc
     6061 acgattgcgg gcaggttaaa ggcgaaaacc atcgcatctt tgatttgcga gaaagcgcgt
     6121 gttgcacgca tggtaatcgc ttcaactttg ttttcttcgc ccggacgatc ggcccagtcc
     6181 ttcaaggaaa cgaacgcaat accggtattc tgaccacgtc ccgcaaagcc gaagccgtta
     6241 acggcgaaca ccgactcaac gttgttcttt tctttggtca gatagtaatg cgttacctca
     6301 ttgagcactt tctgcgtacg ttcctgcgtt gcacctgctg gcagctgaac catggtcata
     6361 aatacgccct ggtcctcatc tggcaagaag gagcttggca gacgcacgaa cagataggcc
     6421 atgccgacca cgatgatcag atacagcact aggtaacgcc ccgtactgcg cagaataccg
     6481 cctacgctgt cggtgtagtg gtgcgtgctc ttctcgaaca tgcggttcaa ccagccgaag
     6541 aagccttttt taccttcccc gtgatcgcct ttggcaatcg gtttcagcat ggtggcacaa
     6601 agagccggag tcaggatcaa cgctaccagt accgacagcg ccattgctga aacaatggta
     6661 atagagaact gacgatagat agcaccagta gaaccgccaa agaaggccat cggtacgaat
     6721 accgccgaca gtaccatcgc gataccgacc agagcgccct gaatctgccc catcgactta
     6781 cgggtagctt cttttggcgg caaaccttct tccgccataa cacgctccac gttttctacc
     6841 acaacgatag cgtcatccac caacaagccg atggcgagca ccatcccgaa cattgttagc
     6901 gtgtttatcg agaagccaaa ggcggcaagg acggcaaagg tcccgagcaa taccaccggt
     6961 acggcaatgg tcggaatcaa cgtcgcgcgg aagttctgca ggaacagata cataaccagg
     7021 aacacgagga tgatcgcttc gaccagcgtt ttaaccactt cgtgaataga gattttcacg
     7081 aacggcgtgg tgtcgtacgg gtaaacaatt ttcagacccg acgggaagaa cggttccatc
     7141 ttcgccagtt cagcacggat tgccgcagcg gtatccagcg cgtttgcacc ggtcgccagc
     7201 ttgatcccca gaccggaagc aggttggccg ttaaactctg cgatgatgtc gtagttctca
     7261 ccacccagct caatcttcgc cacatcacgc agcagtacgc gggaaccatc ctgattcact
     7321 ttcagcagga ttttgccgaa ctcttcagta gaggtcagac gcgtctgagc aataatagag
     7381 gcgttaagct gttggccttt caccggcggc gtaccaccga gctgacccgc cgcaacctgg
     7441 gcgttctgcg ctttgatggc ggtaatgaca tcaaccggcg ttagctggaa tttgttcagc
     7501 tcattcgggt tcatccagat acgcatcgcg tactgtgaac cgaacaactg aacatcaccc
     7561 acgcccgacg tacggctgat ggcatctttc atattcgccg ctacgtagtc agagatatcc
     7621 tcctgcgtca tggtgccatc ggtgttgata acgccgacaa ccatcaggaa gctgctggat
     7681 gatttctcaa cgctcacccc ttgctgctga acttcttgcg gcagcaacgg catcgccagc
     7741 tgcagtttgt tctgcacctg aacctgcgcg atatccgcat cagtaccaga ctcaaaggtc
     7801 agggtgatct gcacggtacc cgtggagtca ctgttagagg acatgtacat caggttatcg
     7861 ataccgttca tattctgttc gataacctgt gtcaccgtgt cctgcactgt tttcgcatca
     7921 gcgccggggt aggaggcgga gatcgttact gccggcggtg caatcgtagg atattgcgcc
     7981 accggcagtt tgaggatcgc cagcccccct gccaacatga tgataatggc gatcacccac
     8041 gcaaaaatcg ggcgatcgat aaagaaatta ggcatgtctt aacggctcct gtttaagtta
     8101 agacttggac tgttcaggct gagcaccgct tgcggcttgc tggttattat cagcggtaac
     8161 ttcttgtgct tttacctgga caccaggacg cactttctgc agcccactta ttactacgcg
     8221 atcgcctgct ttcagacctt ctgtcaccag ccacttatcg ccaatagcct ggcttgcaac
     8281 gatcggacgg gtttccactt tgtcatccgc gccaactacc agtacggtgg catcaccacg
     8341 cggcgtacgg gttacgccct gttgcgggac taaaatagcg tttggattaa gcccttcttc
     8401 cagacgtgcg cgcacgaaca tacccggcag cagagtgtga tccgggttcg ggaagatagc
     8461 gcgtagggtg atagacccag tggtctgatc aacggtaacg tcagagaatt ccagcgtacc
     8521 gtcctgcggg aacttaatgc cgtcactggt gatcagcgac actttggctt tgccgttctc
     8581 ttgtttcagc gtgccattcg ccagttcctg tttcaggcga aggaagtcgt tgctggactg
     8641 ggtcacatca acgtagatcg gatcaagttg ctgcacggtt gccagcacag tcgcctgacc
     8701 gttctgtacc aatgcgcctt ccgtcacgtt cgacttacca atgcgaccgc taatcggaga
     8761 ggtgactttg gtgtaagcca gattgatccg cgcagtttca acggcagctt tcgccgcagt
     8821 taccgcagca ttcgcctgtt gcgcatcagc cagagcctga tcgtaccctt gcttactgat
     8881 gtactgagta ccgagcagtt tctgataacg attcaccgtc aattgcgcga tattggctgc
     8941 agcctgggct ttcgccagat cacctttcgc actgtcgtat gtcgcctgat aggtcgcagg
     9001 atcaatctga tagagagaga caccagcttc gatgtcgcta ccttctttga aattacgctt
     9061 caggataatc ccgctaactt gaggacgaac ttctgcgatc cggtaggcac tggtgcgacc
     9121 tggaagctcg gttgtgatct gcagaggttc agttttgact gttactacgc caacggcggg
     9181 catctgctgg ccaccttgtt gggcctgttt gtcgtcacat cctgttaggg ctaagctgcc
     9241 tgagagcatc agaacgaccg ccagaggcgt aaaccctctg tttttgttca tatgtaaacc
     9301 tcgagtgtcc gatttcaaat tggtcaatgg tcaaaagtta ataaacccat tgctgcgttt
     9361 atattatcgt cgtgctatgg tacatacatt cacaaatgta tgtaaatcta acgcctgtaa
     9421 attcacgaac atatggcacg aaaaaccaaa caagaagcgc aagaaacgcg ccaacacatc
     9481 ctcgatgtgg ctctacgtct tttctcacag cagggggtat catccacctc gctgggcgag
     9541 attgcaaaag cagctggcgt tacgcgcggt gcaatctact ggcattttaa agacaagtcg
     9601 gatttgttca gtgagatctg ggaactgtca gaatccaata ttggtgaact agagcttgag
     9661 tatcaggcaa aattccctgg cgatccactc tcagtattaa gagagatatt aattcatgtt
     9721 cttgaatcca cggtgacaga agaacgacgt cgattattga tggagattat attccacaaa
     9781 tgcgaatttg tcggagaaat ggctgttgtg caacaggcac aacgtaatct ctgtctggaa
     9841 agttatgacc gtatagaaca aacgttaaaa cattgtattg aagcgaaaat gttgcctgcg
     9901 gatttaatga cgcgtcgggc agcaattatt atgcgcggct atatttccgg cctgatggaa
     9961 aactggctct ttgccccgca atcttttgat cttaaaaaag aagcccgcga ttacgtcgcc
    10021 atcttactgg agatgtatct cctgtgcccc acgcttcgta atcctgccac taacgaataa
    10081 ccctgaatct gactccagga tttttcctgg acattttcgt cgttgctatt ctggttcact
    10141 gcgtcgtgat attcttgcgg tttgactttt tcaggtcgtt cttcaggttc agaaaccttc
    10201 attcatcatg actatgttcc agtattacaa acgatcacgg cattttgttt tttcagcatt
    10261 tattgctttt gtttttgtct tgttatgcca gaacacggcg tttgcgcggg cgtcatcgaa
    10321 tggtgatctg ccgacaaaag cggacctgca ggcgcaactt gactcactaa ataaacaaaa
    10381 agatctttct gctcaggaca aactggtgca gcaggatctg acagatacat tagccaccct
    10441 cgataaaatc gatcgcataa aagaagagac agttcagcta cggcaaaaag tcgctgaagc
    10501 gccggaaaaa atgcgccagg cgaccgcggc gttaacagca cttagcgatg tcgataacga
    10561 cgaagaaacg cgcaaaattc tgagtacgct ttcgttgcgc cagctggaaa ctcgcgttgc
    10621 ccaggcgctg gacgatttgc aaaacgcaca aaacgatctg gcgtcttata acagccagct
    10681 ggtttcgtta cagacgcagc ccgaacgcgt gcaaaatgcg atgtataacg cttcgcagca
    10741 gctgcaacaa attcgcagtc gtctggatgg gactgatgtc ggcgagactg ccttacgtcc
    10801 cagccagaaa gtgttaatgc aggctcagca ggcgttgctg aatgcggaga ttgaccagca
    10861 gcgtaaaagc ctggaaggga acaccgtctt gcaggatacc ttgcaaaagc aacgtgatta
    10921 cgtgacggcg aacagcgccc gtctggagca ccagttgcag ctgttgcaag aagcggtaaa
    10981 cagcaagcgc ctgactttaa ctgaaaaaac ggcgcaggaa gccgtctccc cggatgaagc
    11041 cgcgcgtatt caggctaatc cgctggtgaa acaggaactg gaaattaacc agcagttaag
    11101 tcagcgtctg attaccgcga ctgaaaacgg taatcagttg atgcagcaaa acattaaagt
    11161 caaaaactgg ctggagcggg cgctgcaatc ggaacgcaat attaaagagc agattgccgt
    11221 cctgaagggc agcctgctgt tgtctcgtat cctttaccag caacaacaaa cgctgccctc
    11281 ggcggatgaa ctggaaaaca tgaccaaccg catcgcggat ttgcgtctcg aacagtttga
    11341 agttaaccag cagcgtgatg cactcttcca gagcgatgcg ttcgtcaaca aactggaaga
    11401 aggtcacacc aacgaagtca acagcgaagt tcacgatgcg ttattgcaag tggttgatat
    11461 gcgtcgcgaa ttgctggatc aactcaacaa acagttgggt aaccagctga tgatggccat
    11521 taacctgcaa atcaaccagc agcagttaat gagcgtatcg aaaaacctga aatccatcct
    11581 gactcagcaa atcttttggg tgaacagtaa ccgtccaatg gactgggact ggatcaaagc
    11641 gttcccgcaa agcctgaaag atgaatttaa gtcgatgaaa atcacggtga actgggaaaa
    11701 agcctggcct gccgttttta tcgctttcct cgccggtttg ccgctgctgt tgattgccgg
    11761 gctgatccac tggcgtctgg gctggctgaa agcgtatcaa cagaaactgg cttccgctgt
    11821 gggttccctg cgtaacgata gccagctcaa cacaccaaaa gcgatcctta tcgacctgat
    11881 ccgcgcgctg ccggtgtgcc tgattattct cgcggttggc ctgattctgt tgaccatgca
    11941 gctcaacatc agcgaactgc tatggtcgtt cagtaaaaaa ctggcgattt tctggctggt
    12001 gtttggcctg tgctggaagg tactggagaa aaatggcgtt gccgtgcgtc acttcggcat
    12061 gccggagcag cagaccagcc actggcgtcg gcaaattgtc cgcatcagtc tcgcgttgct
    12121 gcctatccat ttctggtctg tggtggcaga actttccccg ctgcatctga tggatgatgt
    12181 gctggggcag gcgatgattt tcttcaacct gctgctgatt gccttcctgg tatggccaat
    12241 gtgccgcgaa agctggcgtg ataaagagtc gcacaccatg cgactagtca ccattaccgt
    12301 gctgtcgatt atcccgattg cattgatggt gctgactgcc acgggttatt tctacaccac
    12361 gctgcgcctc tccgggcgtt ggattgaaac cgtttatctg gtgatcatct ggaacttgct
    12421 ctaccagacg gtattgcgcg gcttaagcgt agcggcgcgg cgtatcgcct ggcgtcgtgc
    12481 gctggcgcgt cggcagaatc tggtgaaaga gggcgcagaa ggtgccgaac cgccggaaga
    12541 acccaccatt gcactggagc aagttaacca gcagacgctg cgtattacca tgttgctgat
    12601 gtttgcgctg ttcggtgtca tgttctgggc aatttggtcc gatttgatca ccgtgttcag
    12661 ctatctcgac agcatcacgc tctggcatta caacggcact gaagctggcg ctgcggtggt
    12721 gaaaaacgtc accatgggca gtctgttgtt tgcgattatc gcctcaatgg tggcctgggc
    12781 gttgattcgc aacctgcctg gtttactgga agtgctggtg ctctcgcgac tgaatatgcg
    12841 ccagggcgcg tcgtatgcca ttactaccat ccttaactac atcattattg ccgttggtgc
    12901 gatgacggtg ttcggatcgc tgggcgtctc ttgggataaa ctccagtggc tggccgcagc
    12961 attatccgta ggtcttggtt ttggtttaca agaaattttc ggtaacttcg tctccggttt
    13021 gatcattctg ttcgaacgtc cggtgcgtat tggcgatacg gtaaccattg gtagcttctc
    13081 ggggacggta agtaagatcc gtattcgtgc gacaacgatt accgatttcg atcgcaaaga
    13141 agtgatcatc ccgaacaaag cgtttgttac cgagcgtctg atcaactggt cgttgactga
    13201 cactactacg cgtctggtga tccgtctcgg cgtggcctat ggctccgatc tggaaaaagt
    13261 gcgtaaagtg ttactgaagg cggcgactga gcacccaagg gtgatgcacg aaccaatgcc
    13321 ggaagtcttc tttacggcat ttggtgccag cacgttggat catgagctgc gtctgtatgt
    13381 gcgtgaactg cgtgaccgta gccgtactgt cgatgagctg aaccgtacta tcgatcagct
    13441 gtgccgtgaa aacgacatca acattgcctt taaccagctt gaagtgcatc tgcacaacga
    13501 gaagggcgat gaggtgacgg aagtaaaacg cgactacaaa ggcgacgatc cgacaccagc
    13561 ggttgggtaa taaccaaagg gggcgacatt tagttgcccc gagattactg acaaagtgcg
    13621 cgttgttcat gccggatgcg gcgtgaccgc cttatccggc ctacgaaacc gcaagaattc
    13681 aatatattgc aggagcggtg taggcctgat aagcgtagcg catcaggcag ttttgcgttt
    13741 gcccgcaatc ttaggggaca tttagcggcc ccatttattt ctcacttttc cgcctcatca
    13801 tcgtgcgtta atttcttttc ataatcacgc tttacaatat ccagcgcgcg cagcacggta
    13861 ctggcaggga tctgattttc ctccagcagc acaatcaaat cgacagccag tttgacatcg
    13921 tcaggggcat tttcaagtga catactcttt ccattgctag cgggttaagc gcgctagcct
    13981 gttttcgatt ttttccagcg catggcggca gcgtgccagg cgcgcttcat aggcttccac
    14041 ttcacgatgc agcgtttgct gttccacgag atcggtcacc cgcgccaggc gggctctgcg
    14101 ttcggcaacc atctcacgta gccgccgctc aaaatcctga tgctgaatac gtttacgctg
    14161 ccagcgggaa attttcggtg gcgcactgtc ccactcgcgc aatgaccagg cggaggcttc
    14221 acgcgcgatg gcttccagtt gtgccgccag atgttccgcc agccaggcca cttgcggcag
    14281 ctgttgctgc tcaactgcat gacgaagcgc agccagatta tcgcccgcct cgtcgagaca
    14341 agactgtagt gttgtcgcac gagtctgaaa aagatgcctg tcgaaacgag cacttagcgt
    14401 ggcgaactgt gacaccgggg cacaacgctg acgcagcgta gcgagctgtc cttccagttt
    14461 ttccagcagc agggcggttt tcacgataag acctcaatga aaatgataat tgttatgcta
    14521 aagtagccac tatgtaagct gacaacactc aatatgcaac gaatcatttt aatcatcatt
    14581 ggctggctgg cggtagtgct gggtacgctg ggcgtggtat taccggtatt accgacgacg
    14641 ccgtttatcc tgctggcggc ctggtgcttt gcccgttctt ccccgcgctt tcacgcctgg
    14701 ttgctgtacc gctcatggtt tggcagctat ctacgtttct ggcagaaaca tcatgcgatg
    14761 ccgcgcggcg tcaaaccgcg ggcgattttg cttattttgc tcacgtttgc catttctctg
    14821 tggttcgtcc agatgccatg ggtgcgcatc atgttgctgg taattctcgc ctgtttgctt
    14881 ttctatatgt ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg aagcacaaca
    14941 atcgcagttg caattattgc gtacagccag tacattctgg cgttttcgag cacaggcgca
    15001 ggcggtcaaa ggttaaacaa cagttacttt tgatacgttt aaaacgcgcc gtgagtacca
    15061 ccgtaacaag caggcataca cttatgaccg cgactgcaca gcagcttgag tatctcaaaa
    15121 atagcatcaa aagcattcag gactacccaa aacccggcat tcttttccgc gatgtcacca
    15181 gcttactgga agacccgaaa gcttacgctc tcagcatcga cttgctggtt gagcgttaca
    15241 aaaatgcggg cattaccaaa gttgtcggca ccgaagcgcg tggcttcttg tttggcgctc
    15301 cggtagctct gggtctgggc gttggctttg taccggtccg taaaccgggc aaactgccgc
    15361 gtgaaaccat cagtgaaaat tacgacctgg aatacggcac cgatcagctg gaaatccacg
    15421 ttgatgccat caaaccgggc gacaaagttc tggtggtgga cgatctgctg gcaaccggcg
    15481 gcactatcga agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg gctgacgctg
    15541 cgttcattat caacctgttt gatctcggcg gcgaacagcg tctcgaaaaa cagggcatta
    15601 ccagctacag ccttgtcccg ttcccgggcc attaattatc gccagtcttg tgctgcccac
    15661 gctacggaca gcacaagatg tgcattcagc ctcgccgttc tgacggggct gtgttagcat
    15721 taccccttcg tgaatccacc ttccagcgtt tcagagcctg ccaatgagtt atcaggtttt
    15781 agcccgaaaa tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac atgtgctgac
    15841 cgcactggcg aacggcttgt cgttagggcg tattcatcat gcttatcttt tttccggcac
    15901 ccgtggcgtc ggaaaaacct ctatcgcccg actgctggcg aaggggctaa actgcgaaac
    15961 cggcattacc gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg agcaggggcg
    16021 ctttgtcgat ctgattgaaa tcgacgccgc ctcgcgcacc aaagttgaag atacccgcga
    16081 cctgctggat aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt atctgatcga
    16141 cgaagtgcat atgctgtcgc gccacagctt taacgcactg ttaaaaaccc ttgaagagcc
    16201 gccggagcac gttaagtttc tgctggcgac gaccgatcca cagaaattgc cggtgacgat
    16261 tttgtcacgc tgtctgcaat ttcatctcaa ggcgctggat gtcgagcaaa ttcgccatca
    16321 gcttgagcac atcctcaatg aagaacatat cgctcacgag ccgcgggcgc tgcaattgtt
    16381 ggcgcgcgcc gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc aggcgattgc
    16441 cagcggtgac ggccaggttt caacccaggc ggtcagtgcg atgctgggta cgcttgacga
    16501 cgatcaggcg ctgtcgctgg ttgaagcgat ggtcgagggc aacggcgagc gcgtaatggc
    16561 actgattaat gaagccgctg cccgtggtat cgagtgggaa gcgttgctgg tggaaatgct
    16621 cggcttgttg catcgtattg cgatggtaca actttcgcct gctgcacttg gcaacgacat
    16681 ggccgccatc gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga cggatattca
    16741 gctttactat cagacgctgt tgattggtcg caaagaatta ccgtatgcgc cggaccgccg
    16801 catgggcgtt gagatgacgc tgctgcgcgc gctggcgttc catccgcgta tgccgctgcc
    16861 tgagccagaa gtgccacgcc agtcctttgc acccgtcgcg ccaacggcag taatgacgcc
    16921 aaccctggtg ccgccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac
    16981 caccagccag gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc
    17041 aaaaaagagt gaaccggcag ccgctacccg cgcgcggccg gtgaataacg ctgcgctgga
    17101 aagactggct tcggtcaccg atcgcgttca ggcgcgtccg gtgccatcgg cgctggaaaa
    17161 agcgccagcc aaaaaagaag cgtatcgctg gaaggcgacc actccggtga tgcagcaaaa
    17221 agaagtggtc gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga
    17281 actggcggtg aagctagcgg cagaagccat tgagcgcgac ccgtgggcgg cacaggtgag
    17341 ccaactttcg ctaccaaaac tggtcgaaca ggtggcgtta aatgcctgga aagaggagag
    17401 cgacaacgca gtatgtctgc atttgcgctc ctctcagcgg catttgaaca accgcggtgc
    17461 acagcaaaaa ctggctgaag cgttgagcat gttaaaaggt tcaacggttg aactgactat
    17521 cgttgaagat gataatcccg cggtgcgtac gccgctggag tggcgtcagg cgatatacga
    17581 agaaaaactt gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg
    17641 tcggtttttc gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca
    17701 gcttacgttc gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc
    17761 tgggtaacct gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag
    17821 agatcgcgca gctggaagtt accggcgaat ctggcgcagg tctggtaaaa gtgaccatca
    17881 acggtgcaca caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag
    17941 agatgctgga agacctggtg gctgcagcat tcaacgacgc agcacgtcgt attgaagaaa
    18001 cgcagaaaga aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga
    18061 tgccgttctg atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct
    18121 gccgggcgtt ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg
    18181 tagcggcggg atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg
    18241 cgccgattgc cgcactttca ccgaacagga agtctgtaac atctgttcga atccgcgtcg
    18301 tcaggaaaac ggtcaaatct gtgtggtgga gagtccggcg gacatctacg ccattgagca
    18361 gacggggcag ttttctggtc gttattttgt gttgatgggg catctgtcac cgctggacgg
    18421 catcggtccg gatgatatcg ggcttgatcg tctggaacag cgtctggcag aggaaaaaat
    18481 cactgaagtg atcctcgcca ccaatcccac ggttgaaggt gaagctaccg ctaactacat
    18541 tgccgagctt tgcgcgcaat atgacgtgga agccagccga atcgctcatg gcgtaccggt
    18601 tggcggcgaa ctggaaatgg tcgatggcac cacgctgtca cactccctcg ccgggcgtca
    18661 taagattcgt ttttaagcaa acgagagcag gagcacctgc tctcgcttga aattattctc
    18721 ccttgtcccc atctctccct catcctgttt ttaaccttaa aatggcatta ttgaggtaga
    18781 cctacatgaa aggacaagaa actcgtggtt ttcagtcaga agtgaaacag cttctgcacc
    18841 tgatgatcca ttctctctat tccaataaag aaatcttcct gcgtgagctt atctctaacg
    18901 cctccgatgc ggcggacaag ctgcgtttcc gtgcgctatc taacccggac ctgtacgaag
    18961 gtgatggcga actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg ctgaccatct
    19021 ccgataacgg cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta
    19081 aatccggtac caaatcattc ctcgaatccc tgggttctga ccaagcgaaa gacagccagc
    19141 tgatcggtca gtttggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgaccg
    19201 tgcgtactcg cgcggcaggc gaaaaaccag aaaacggcgt cttctgggaa tcggctggcg
    19261 aaggtgaata taccgttgcc gatatcacca aagaagatcg tggtactgaa atcaccctgc
    19321 atctgcgtga aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcatcagca
    19381 aatactccga ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagatggcg
    19441 aaaccgttat ctcctgggca aaaatcaaca aagcgcaggc gctgtggact cgtaacaagt
    19501 cggaaatcac cgatgaagag tataaagagt tctacaaaca catcgcccac gactttaacg
    19561 atccgctgac ctggagccac aaccgcgttg aaggtaagca ggagtacacc agcctgctgt
    19621 acatcccgtc ccaggctccg tgggatatgt ggaaccgtga tcataaacac ggcctgaaac
    19681 tgtacgttca acgcgtgttt atcatggacg acgctgaaca gttcatgccg aactatctgc
    19741 gcttcgtgcg tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc
    19801 tccaggacag cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa
    19861 tgctggaaaa actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg
    19921 gcctggtact gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac
    19981 tgctgcgttt tgcttctact tataccgatt cttctgcgca gaccgtgtct ctggaagact
    20041 acgtttcccg catgaaagaa gggcaggaga aaatctacta catcactgca gacagctatg
    20101 cggcagcgaa gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc
    20161 tgctttccga ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac
    20221 cgttccagtc ggtgtctaaa gttgacgagt cgctggaaaa actggctgac gaagttgatg
    20281 agagcgcgaa agaagcggag aaagcactga ctccgttcat cgaccgcgtg aaagctctgc
    20341 tcggcgagcg cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg
    20401 tttcgaccga cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc
    20461 agaaagtgcc agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac
    20521 gtgcggcaga tactgaagat gaagccaagt tcagcgagtg ggtagaactg ctgctggatc
    20581 aggcgctgct ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga
    20641 atcagctgct ggtttcctga tgtcatgccg gatgaccttt gtgtcatccg gcatttttct
    20701 tttcatcatc tgtactttcc gcaaattatc tcgccattaa ccgtttcagc ctcaggtgcc
    20761 tttcttgagg caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aaatcgacac
    20821 attttaaggg gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag
    20881 ggactcaggc tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata
    20941 tgctgcgtgc tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg
    21001 atgctggcaa actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc
    21061 aggaagactg ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag
    21121 acgcgatgaa agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg
    21181 aactgattgt tgatcgtatc gtaggccgcc gcgttcatgc gccgtctggt cgtgtttatc
    21241 acgttaaatt caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga
    21301 ctacccgtaa agacgatcag gaagaaaccg tgcgtaaacg tctggttgaa taccatcaga
    21361 tgactgcacc gctgatcggc tactactcca aagaagcgga agcgggtaac accaaatacg
    21421 cgaaagttga cggtaccaag caggttgctg aagttcgcgc tgatctggaa aaaatcctcg
    21481 gctaatcaag ccgcagcaga tctgctttcg ggcaggtctg cttatccacc tcaggcaata
    21541 cctctcacag caattagttc ttcttcctca cttttccgct acaattatca acaagttgaa
    21601 tcgataagag gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctgggtac
    21661 gcccgatgcc cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag
    21721 acgcgtggtt gatacctcac ggttgttatg gtggccgttg ctgcgcggcg tgattttgcc
    21781 gctgcgctcg ccgcgtgtgg cgaagctgta cgcctctgtc tggatggaag acggctcgcc
    21841 gctgatggtt tacagccgcc agcaacagca ggcgttggca caacgtttac cggatacgcc
    21901 agtggcgctg ggcatgagct acggctcgcc atcactggaa agctccgtcg atgaactcct
    21961 ggcagagcat gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttctac
    22021 ggtcggtgcg gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg
    22081 gatatcgttt attcgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag
    22141 cgtacgcgct tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg
    22201 cattccccag cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacgac
    22261 tcgcgaactg gcttccgcac tggggatggt accggaaaaa gtgatgatga cctttcagtc
    22321 gcgctttggc cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg
    22381 agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg attgtctgga
    22441 gacgctggaa gagattgccg agcaaaaccg tgaggtcttc ctcggtgcgg gcgggaaaaa
    22501 atatgaatat attccagcgc ttaatgccac gccggaacat atcgaaatga tggctaatct
    22561 tgttgccgcg tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc gaagagcctc
    22621 gtcggcggtt ttcatcatcc gtgaataatg caaaaaggca tgcagcgtac ctgggtagag
    22681 tttgaactca cagggctgct gatgcgccgc taacgtctag taaagcagac ggctgtcatc
    22741 cagcagcgga tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc
    22801 attattaaac agacagtaat acggtgactc gcggtccgcg tcgttgctta aatacgcctc
    22861 ttcatacatc tgcaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg
    22921 agtcacggaa tcccgtaatc cgtaaagccc ataccacagc aaaacacccg caattttacc
    22981 gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg agcgccagca tggcacctgc
    23041 ggaatcaccg gcaaagccaa tgcgggacat attgatttga taatcctccg cctgctggtg
    23101 gaaataacaa caagcagcca caatttcctc tatcgcttgt ggaaaacgcg cttcaggtga
    23161 aagggtgtaa ttaataccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat
    23221 gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat aaaatagcgt
    23281 cgccgggcta tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg
    23341 aaccatgtaa gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag
    23401 cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg gtaaatccgg
    23461 ctgaagagta ttcacaacgg tcttcatttc agcagaaata aggtccagaa caggtagttt
    23521 gttttccggc ttcatacaaa aactcctttc aaattacgtc attgtaagga accactgcca
    23581 tgaaaatgcg atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag
    23641 attgtgttaa cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg
    23701 taaacgtaaa tccaaacatt acttccctgt aaatgcacgc gatccgctgc ttcagcaatt
    23761 ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga cgctggtcga
    23821 tattgaagcg aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc
    23881 actggtgatt gaggatgacg tagccgaagt gctttatcag gagctaaaac agaaaaacct
    23941 gattacccat cagtttgcgg gtggcaccat tggtaacacc atgcacaact actcggtgct
    24001 cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagtta
    24061 tgcctatcgt tacctgtgta acacttccag ccgtaccgat cttaactatc tacaaggcgt
    24121 ggatggtccg attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc
    24181 tatcagtcca ggccacatga accagctgcg ggctgaaagc attccggaag atgtgattgc
    24241 cggagcctcg gcactggttc tcacctcata tctggtgcgt tgcaagccgg gtgaacccat
    24301 gccggaagca accatgaaag ccattgagta cgcgaagaaa tataacgtac cggtggtgct
    24361 gacgctgggc accaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa
    24421 agaccacgtt tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag
    24481 cgatccgttg ttggcatctg acaaggcgct ggactgggta gagctggtgc tgtgcaccgc
    24541 cgggccaatc ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca
    24601 gcatccgctg ctgccgggcg ctatagcgga attcaaccag tatgagttta gccgcgccat
    24661 gcgccacaag gattgccaga atccgctgcg tgtctattcg cacattgcgc cgtacatggg
    24721 cgggccggaa aaaatcatga acactaatgg agcgggggat ggcgcattgg cagcgttgct
    24781 gcatgacatt accgccaaca gctaccatcg tagcaacgta ccaaactcca gcaaacataa
    24841 attcacctgg ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag
    24901 ctatcaggta ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg agcgtgaaga
    24961 cagccaggaa gagtcttact gggatcgtta agttatcgtc ggttcgtagg ccagataagg
    25021 cgttcacgcc gcatctggca tttggctctc gatgcctgat gcgacgctgg cgcgtcttat
    25081 catgcctaca tatttttcat attttacatc cggcaaccac cgtttacccc gtcaccactt
    25141 cacccgccgg tggcgtttcc agcagttcca gcatagtacg ggcaatttca cgttcaccca
    25201 tcactacctg attcgcgcca cgttcggtga tatacgtcac ttcatcgtca taatgggcgc
    25261 gggcgataat ctcaatatcc gggtttttcg cgcgggcaga cgccacaatc tcacccgctt
    25321 cataaccgtt ggggatcgtc aggatcagcc attttgcaca ttccagatgt gccagttgca
    25381 taatttcttc gttcgctgca ttgcccaata cagcgcggac cccacgctct cgcagctcat
    25441 caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg agcaatttct
    25501 cccccagcag gctgcctaca cgaccgtaac ccaccaatag cgcatggttg caaatatcca
    25561 ctgggatctg tttctcttct tcgattgcct cttccagcgt ctgctcttcc agcgtttcgg
    25621 tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata atcgacagga
    25681 tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat gccattccca
    25741 gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg atggttaatg
    25801 ccgtacgttg ggagtgacca aacagacgga caaggaacag ggccgcgacc gatttaccaa
    25861 acagaataat tgccagcgtc gccagcactg ccagcggttg ctgaatcaga attaacggat
    25921 caaacaacat cccgactgag acaaaaaaca gcaccgcaaa cgcgtcgcgc aatggcaacg
    25981 tatcgtgggc ggcacggtga ctcaattcag actcgttcag taccatcccg gcaaagaacg
    26041 caccgagtgc aaaggagaca tcaaacagct ctaccgcacc aaaggcaatc cctaacgcca
    26101 gcgccagcac tgacagggta aacagctcgc gagaaccggt tgccgcgctg cgtgccataa
    26161 tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg atcaccttgc
    26221 cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc tgttccatca
    26281 ttcctgccac tgcgggcagc aacaccagcg tcagaaccat caccaggtct tccacaatca
    26341 accaaccgat ggcgatttgc ccacgctgac tgtctattaa ttgccgttct tcaagtgcgc
    26401 gcagtaacac cacggtactg gcggtggaaa gacataagcc gaacacgata ccggtcatta
    26461 acgaccagcc cagcacggcg gagagcgcca tacccagcag cgtcgccacg gctatctggg
    26521 cgatcgcgcc gggaatggcg atggacttta ccgccatcaa atccttgagc gaaaaatgca
    26581 aaccgacgcc gaacatcagc aaaataacgc ccagttcagc cagttccggc gcaagcttgg
    26641 tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac agatatccca
    26701 ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg agcacaaggc
    26761 cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct tttcctggtg
    26821 gttattgtcc atttttggct gggaaaacca aaattacagg taatagttta tgacaatttc
    26881 attgatgatg tttatgaata attgttgaat tttgcagaaa aatggaatta gctgcaaaaa
    26941 aagcacggtt cggaaaacag aaggcggttt aacagaggaa aggcttatgg cgactgtaaa
    27001 acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc cggttatcag
    27061 gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta tagactaact
    27121 cgatgctggt gtgatcggcg ataagcccca gaactgccgc tcccagacct cccatgccga
    27181 aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagt agctcctgag
    27241 cgtagaccag aatggcagag aatgccgaag cgaggataaa tccaataatc accgttaaaa
    27301 cccccgtcca gtgcaggcgg gcgtagggta aaatcagcgt aaacggcgca acgccgagga
    27361 tagagcccca aatcacatat ttccgtccaa ttttatcccc tacaggcccg ccgatcaccg
    27421 tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgggcattc tggatagata
    27481 atccgaattt ttgcatcaga taaaaggtgt aatagctgct gatgctcgcc atatagaaat
    27541 atttcgagaa aatgaggatt aacagaatgc tgactgccag taccactttg ttgcgcggca
    27601 gtggattgat tatcgtcgct ttgggttttc ctttattcat tcggtgctgt gccgagtacc
    27661 aacggctaat ttgcgccaac accacgatcg ccagcagtgc cgcaagcaca aaccaggcaa
    27721 cgttgccttt gccataaggc tcgataatca ccgccgccag caagggtccc agggaactgc
    27781 caaagttgcc gccgacctga aagatagatt gcgccaggcc atgccgcccg ccggaagcca
    27841 tacgggctac gcgagaagat tccggatgaa agaccgatga accggtaccg accagcgccg
    27901 ctgccagcag aactgcgcca aaactgcccg ccagcgcaag cagcaccaga ccacttaagg
    27961 taaagcacat gccaattggc aacgaccacg gcatcggata tttatcggtc cagtagccga
    28021 ccactggttg cagtagcgaa gaggcgagct ggaaggtgag ggttatcatg ccaatctgca
    28081 taaatgtcag agaaaattct gactgaagca gcggataaat cgccagaatc agcgattgga
    28141 tcatgtcgtt cagcagatgt gagaggctga tagcacctaa aataccaaac gatgttcggg
    28201 ccttggtcgt tgacgcagcc gcgcccgcca caggctgggt ttgttcactc attgccatag
    28261 gaaagtcact ttttcagggt tgcgatgtaa agaatgatct tatttgtgat tattacccga
    28321 ctaacatacc tgtatgcgtc gtctgaagga agtctcaacg ccgaatacag aatttctaat
    28381 ctggatgcag atttatcttc accggacgca gacttgtcta tgatgttgcg tcatactatt
    28441 tttcaacacg ttgaaatcag gtcagggaga gaagtatgaa attattgcag cggggcgtgg
    28501 cgttagcgct gttaaccaca tttacactgg cgagtgaaac tgctctggcg tatgagcagg
    28561 ataaaaccta caaaattaca gttctgcata ccaatgatca tcatgggcat ttttggcgca
    28621 atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct ggtggatggt atccgcaaag
    28681 aggttgcggc tgaaggcggt agcgtgctgc tactttccgg tggcgacatt aacactggcg
    28741 tgcccgagtc tgacttacag gatgccgaac ctgattttcg cggtatgaat ctggtgggct
    28801 atgacgcgat ggcgatcggt aatcatgaat ttgataatcc gctcaccgta ttacgccagc
    28861 aggaaaagtg ggccaagttc ccgttgcttt ccgcgaatat ctaccagaaa agtactggcg
    28921 agcgcctgtt taaaccgtgg gcgctgttta agcgtcagga tctgaaaatt gccgttattg
    28981 ggctgacaac cgatgacaca gcaaaaattg gtaacctgga atacttcact gatatcgaat
    29041 ttcgtaagcc cgccgatgaa gcgaagctgg tgattcagga gctgcaacag acagaaaagc
    29101 cagacattat tatcgcggcg acccatatgg ggcattacga taatggtgag cacggctcta
    29161 acgcaccggg cgatgtggag atggcacgcg cgctgcctgc cggatcgctg gcgatgatcg
    29221 tcggtggtca ctcgcaagat ccggtctgca tggcggcaga aaacaaaaaa caggtcgatt
    29281 acgtgccggg tacgccatgc aaaccagatc aacaaaacgg catctggatt gtgcaggcgc
    29341 atgagtgggg caaatacgtg ggacgggctg attttgagtt tcgtaatggc gaaatgaaaa
    29401 tggttaacta ccagctgatt ccggtgaacc tgaagaagaa agtgacctgg gaagacggga
    29461 aaagcgagcg cgtgctttac actcctgaaa tcgctgaaaa ccagcaaatg atctcgctgt
    29521 tatcaccgtt ccagaacaaa ggcaaagcgc agctggaagt gaaaataggc aaaaccaatg
    29581 gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca gaccaatatg gggcggttga
    29641 ttctggcagc ccaaatggat cgcactggtg ccgactttgc ggtgatgagc ggaggcggaa
    29701 ttcgtgattc tatcgaagca ggcgatatca gctataaaaa cgtgctgaaa gtgcagccat
    29761 tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga ggtgattgat tacctgaccg
    29821 ccgtcgcgca gatgaagcca gattcaggtg cctacccgca atttgccaac gttagctttg
    29881 tggcgaaaga cggcaaactg aacgacctta aaatcaaagg cgaaccggtc gatccggcga
    29941 aaacttaccg tatggcgaca ttaaacttca atgccaccgg cggtgatgga tatccgcgcc
    30001 ttgataacaa accgggctat gtgaataccg gctttattga tgccgaagtg ctgaaagcgt
    30061 atatccagaa aagctcgccg ctggatgtga gtgtttatga accgaaaggt gaggtgagct
    30121 ggcagtaatc cgaaagtgcc ggatgtttgc atccggcaca atgcttaatc gcggcgggcg
    30181 atatcagcaa atttggcatc gaggatcttt gccagatcgc ctgccgccag ttcgatatcc
    30241 agtccgcgct tgccgccgga aacataaata gtggcaaatt cttgtgcggg ggcgtcgata
    30301 atcgttggca gacgtttttt ctgccccagt gggctaatcc ccccaaccag gtatcccgtc
    30361 gaacgctgcg cgaccatcgg atcggccatc tcaactttct tggcacccag cgcttttgct
    30421 acttttttaa gatccagttg acctgcgacc ggcgtaacgg ccacggcaag gtgtttcata
    30481 tcaccgttca ctgccaccag cagcgttttg tagacctgat ccggattcaa acctaatttt
    30541 ttgacgactt catcgccaaa attggtttca gccggatcgt gctcgtaggt atggatttga
    30601 aacgaaatct tgtttttttc gagtaattta actgcgggtg tcatggtaat tttcctgcct
    30661 taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc caaaaaaaag agtcatcttg
    30721 cctaagagta ttggcaggat ggtgagattg agcgacaatc gagttacacc gtcgcttaaa
    30781 gtgacggcat aataataaaa aaatgaaatt cctctttgac gggccaatag cgatattggc
    30841 cattttttta gcgcaacatt tgcggcaaat tcccttctcc atacaggtgt agtgcaccga
    30901 ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt atcccgccag gcgagatttc
    30961 gctgatgcat cagcacatcg tacagcgact gactgaacgt attgggcagc gttatatcat
    31021 tattttgcgg cggtgcattc agccaccagc tcatcatttg ttgcagcaac cgtgcgttgg
    31081 tatgccagtg ggtcagcgta tcgtccagca gcgccagtcc tttgtcaggg agctggagca
    31141 acatggcaat ctggttttca gccccttcca gttcaatcac gggtttatgt tgttgcttcg
    31201 ccgcctgcaa tagctggtaa tcgataccgt attctgcccg cagccccagt ttttgtgcct
    31261 gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg ggtagaaaag agtgagggag
    31321 aaatgcccat ctcctggcta atgtgctgca ggttttggag ttgctcctcg ctaatgcgct
    31381 cttccagcgc ctcgcaggca ggcaaattag caaaaggcgt atcgctggtg gaaacatccg
    31441 cctcgacgat cagcgcatcg gcgtttttga gctttttgag caaacgggtg ggcaggggag
    31501 ccatatcgtg gctacccata tgaatactgc caatcagatg aaaatggcga ttgccgggga
    31561 gggtgatatc gatggcaggc caggtgtaat gattaccgcg cagcgcggcc caaagcgttt
    31621 ttacccggta caacagatcc atacgacctc cctttgtgaa atatcatgct agcgcgcggt
    31681 gagggatggc gcaaatggag ttggggactt gtttaatgtg tttgtatgat tcagtatgtt
    31741 cttgcatcgc tattcacagg gaagcaacag ttaaaaacca tgaaacaggc aacaagaaaa
    31801 ccgacgacac ccggagatat tcttctctat gaatatctgg aaccgctcga tttgaaaatc
    31861 aatgagttag cagagttgct gcatgttcat cgtaatcgcg tcagtgcact gatcaataac
    31921 aatcgtaaac tcaccactga gatggcattc cgtctggcga aagtttttga taccacagtc
    31981 gattttaggc taaacctcca ggcggcagtt gatctttggg aagttgaaaa caacatgcgc
    32041 acccaggaag aattgggacg gattgaaaca gtggctgaat atttggcacg ccgtgaagag
    32101 cgtgcaaaaa aggtcgcgta aggccaaaac atcgcttcat ctcgtaggcc ggataaggcg
    32161 ttcacgccgc atccggcatc cgagcaccat tgcctgatgc gacgcttacg cgtcttatca
    32221 ggcctacaaa cctgtgcctg aaccgtaggc cggataaggc gtttacgccg catccggcag
    32281 tcatgcgtcg atgccaaatg cgccgcccta aagcagcgca tccgcaatga cgtacttatt
    32341 ccttcggttt aaaccgcagc aaccggttgg cgttacttac tacggtaatt gacgagagcg
    32401 ccattgccgc tccggcaact accgggttaa gcagtgttcc agtaaacggc cacaaaatac
    32461 cggcggcgac cggaataccg atgctgttgt agataaaggc accgagcagg ttctgcttca
    32521 tgttgcgcag cgttgcgcgg gaaatcgcga gcgcatccgc aacgcccatc aggctatggc
    32581 gcatcagggt aatcgccgcg gtttcaatgg caacatcact gccgccaccc atcgcaatgc
    32641 cgacatccgc ctgggccagc gctggcgcgt cgttaatgcc gtcgcccacc attgccacct
    32701 gacgtccttc actttgcaga cgtttgatcg cttcggcttt accgtccggc agcactccgg
    32761 caatcacttc atcaattcca gcttctttgg cgatcgcatt ggcggtggtt gggttatccc
    32821 cggtcaacat caccagacga tatcccgctt tatgcaggcg ttgcagcgcc gccacgctat
    32881 cactacgcaa cggatcgcgt acagccagca gggctactgc tttcccgtca atcgccagca
    32941 gcacaggcgt tgctccttgc gatgcctgag ctgaaatatc ggcttcaatc gctttagtat
    33001 cgacttgctg atcatttaac aacgcctgat tacccagcaa taacgcatga ccttctgctt
    33061 caccgctcac gcccagcccg cgcaatgtgc ggaaaccgtt gacctgcggt agctgcatat
    33121 cgcctgcttt atcaaggatc gctcgtgcca atggatggct ggaaccttgc tccagcgccg
    33181 ccgccagacg cagcgcctgc gcttcgtcaa catcagcaaa tgttttcact gcgacaacct
    33241 gcggcttccc ttcggtcagc gtcccggttt tatcgaacac gacggtatcg agtgtactgg
    33301 cgcgttgcag cgcgtcggcg tcccgcacca gcacgccaaa ctcagccgcc cgcccgacgc
    33361 cggaaataat cgacatcggc gtcgccagtc ccagcgcaca cgggcaggcg ataatcagca
    33421 ccgtagtggc aatcaccagg gtatagacaa tctgcggtgc cggaccaaag aaataccaga
    33481 ttgccgcact gacaagcgca ataaccacta ctaccggcac aaatatggct gagattttat
    33541 ccgccagctg accgatttct ggcttgctgc tctgggcctg gcgcaccatg cgaatgatcc
    33601 ttgatagcgt ggtatggctg ccaaccgcac tggcacgaaa cagcacactg ccgtcctgta
    33661 ccactgtccc ggcatggacg ctatcgcctt cgcctttttg ctgcgggatt ggttcgcccg
    33721 tcagcatcgc ttcatccagc catgcttcgc cctgggtaat ctcgccatct accggcacgc
    33781 gatcgccggt cgtcaggcgc agcaacatac ctggctgcac ttctgccaga ggcacgcttt
    33841 tttcaccttc gtcagtaacc aggcgcgccg tcggtggggt taaatcgagt aatttctcca
    33901 gtgccttaga agaacgctga cgtgcgcgtg cttccagcat atggccgaga ttgatcagac
    33961 cgataatcat cgcactggct tcatagtaaa gatgtcgcgc ttccatcggg aaccactgcg
    34021 gccacaggtt gacgctcatc gaatagagcc acgccacgcc cgttcccagc gccaccagcg
    34081 tatccatcgt cgctgcaccg ttcagcaggc ttttccatgc actgcggtaa aaatggccgc
    34141 tggcgaaaac catcaccgcc agggttatca ggccgataac caaccacagg ctgcggttgt
    34201 cggcggtgac tatcatgtta tcgccgatca tcccccagac catcaccggg atacccaccg
    34261 ccagtgcgac aattgcctgc cagcggaagc gcttcatcgt agcgacggcg gtttcttgct
    34321 ggcgttcgcg gcgtttcgcg tcatcttcaa tcgcttccgc gccgtagccc gctttttcca
    34381 ccgcctgcac taaatcttgt ggggaggcac tgcccatcac cagcgcagta cgctccgcca
    34441 ggtttacccg tgcctgagtg acgcccggta cgctttgcag cgcattttgt acgcgggtga
    34501 cacagctggc gcagctcatg ccgctcagca gcaactgctg gctgtcatcg tcatcggcgg
    34561 tcgctgccgg aagcgcctca gaaaccgctg tcagtgcttc cgacgggatt gatgactccg
    34621 ccagcggttt agcctttggg tggcttacag atgcgtcata acccgcttgt ttgatggttt
    34681 cgatcagctg ttctgcactg gcagtcccgg taacgtgcgc ttcagtgata gacacatccg
    34741 cttgttccac atccgggcgc tgttcaagac tttctttcac gcgtttaacg cagtgaccgc
    34801 aggacaggcc gtccaggttc aggtcgatag tttgtgacat aaaacactcc tttaagacag
    34861 ttttgactgg ctgtgataaa ggttaaacct tccagcaagg ggaaggtcaa gaaattaata
    34921 aaccaggcgg gtaaaagtcc gtaaagatta aaaaatcggc tcgatttgca tcaggattag
    34981 acatttattt ctttgttttc ctgtagttaa gttgcgggtg ctaagttaaa tcaggatgcc
    35041 tgaaaatcgg cactggggtg aggaattacc tcccgcatct ataaaaagga gttaacaaaa
    35101 gatgttagat gcaaacaaat tacagcaggc agtggatcag gcttacaccc aatttcactc
    35161 acttaacggc ggacaaaatg ccgattacat tccctttctg gcgaatgtac caggtcaact
    35221 ggcggcagtg gctatcgtga cctgcgatgg caacgtctat agtgcgggtg acagtgatta
    35281 ccgctttgca ctggaatcca tctctaaagt ctgtacgtta gcccttgcgt tagaagatgt
    35341 cggcccgcag gcggtacagg acaaaattgg cgctgacccg accggattgc cctttaactc
    35401 agttatcgcc ttagagttgc atggcggcaa accgctttcg ccactggtaa atgctggcgc
    35461 tattgccacc accagcctga ttaacgctga aaacgccgaa caacgctggc agcgaatttt
    35521 acatatccaa cagcaactgg ctggtgagca ggtagcgctc tctgacgaag tcaaccagtc
    35581 ggaacaaaca accaacttcc ataatcgggc cattgcctgg ctgctgtact ccgccggata
    35641 tctctattgt gatgcaatgg aagcctgtga cgtgtacacc cgtcagtgct ctacgctcat
    35701 caatactgtt gaactggcaa cgcttggcgc gacgctggcg gcgggtggtg tgaatccgtt
    35761 gacgcataaa cgcgttcttc aggccgacaa cgtgccgtac attctggccg aaatgatgat
    35821 ggaagggctg tatggtcgct ccggtgactg ggcgtatcgc gttggtttac cgggcaaaag
    35881 cggtgtaggt ggcggtattc tggcggtcgt ccctggcgtg atgggaattg ccgcgttctc
    35941 accaccgctg gacgaagaag gcaatagtgt tcgcggtcaa aaaatggtgg catcggtcgc
    36001 taagcaactc ggctataacg tgtttaaggg ctgatcatga tgaacacgga aggtaataac
    36061 ggtaacaaac ccctcggtct atggaacgtc gtttccatcg gtattggggc aatggtgggg
    36121 gcggggatct tcgcgctgct ggggcaggct gcgttgctaa tggaagcctc gacctggggc
    36181 gcctttgctt ttggcggtat tgtggctatg ttttccggtt atgcctatgc gcgtctgggg
    36241 gcgagctatc ccagcaatgg cggtattatc gacttttttc gtcgcggatt aggcaacggc
    36301 gtcttttcgc tagcgctctc gttgctatac ctgttgacgc tggcggtgag catcgccatg
    36361 gttgcccgtg cttttggcgc ttatgccgtg cagtttttgc atgaaggcag ccaggaggag
    36421 caccttattt tgctctacgc gttggggatc attgcggtga tgacgctttt caactcctta
    36481 agcaaccatg cggtagggcg gctggaagtg atcctcgtcg gcattaaaat gatgatcctg
    36541 ttattgctga ttattgccgg tgtctggtcg ctgcaaccgg cgcatatttc cgtctctgcg
    36601 ccccccagct ccggtgcgtt cttctcctgt attgggataa ccttccttgc ctatgcgggc
    36661 tttggcatga tggcgaacgc ggcggataaa gtgaaagatc cgcaggtcat tatgccacgg
    36721 gcgtttctgg tggcgattgg cgttaccacg ttgctttata tctcgctggc gctggttttg
    36781 cttagcgatg tatcggcatt agagttagaa aaatatgctg ataccgccgt agcgcaggct
    36841 gcttctccgc tgctcgggca tgtgggttat gtgatcgtcg tcatcggcgc tttactggcg
    36901 acggcttcag gcattaacgc gaacctgttc gccgtgttta acatcatgga caacatgggc
    36961 agcgaacgcg aactgccgaa gctaatgaat aaatccctgt ggcggcagag tacctggggc
    37021 aatatcatcg tcgtggtgtt gataatgctg atgacggcgg cgctaaattt agactcactc
    37081 gccagcgttg ccagcgccac ctttttgatc tgctatctgg cggtgtttgt ggtggcgatc
    37141 cgcctgcgtc atgatattca cgcctcgttg ccgattctta tcgttggtac gttggtgatg
    37201 ttgttggtga tcgttggctt tatctacagc ctgtggtccc agggcagccg tgcgttgata
    37261 tggattattg gtgcaatctt actcagcctt attgtggcaa tggtcatgaa gcgtaataaa
    37321 accgtataac acctctctgt gcgcagtact tcctgtatta ttgtggtggc ggtcgatatt
    37381 cgcactggca aaaaaacgtg cttgaatatc tgttgaaacc ctttaacaaa gcacaggagg
    37441 cgttgcgcga acgatgaaca tcagcgatgt agcaaaaatt accggcctga ccagcaaagc
    37501 cattcgcttc tatgaagaga aggggctggt gacgccgccg atgcgcagcg aaaacggtta
    37561 tcgcacctac acgcagcagc atctcaacga actgacctta ctgcgccagg cacggcaggt
    37621 gggctttaac ctggaagaga gcggcgagct ggtgaatctg tttaacgacc cgcagcggca
    37681 cagcgccgac gtcaaacggc gcacgctgga gaaggtggcg gagatcgaac gacacattga
    37741 ggagctgcaa tccatgcgcg accagctgct ggcactggcg aatgcctgcc ctggcgatga
    37801 cagcgccgac tgcccgatta tcgaaaatct ctccggctgc tgtcatcatc gggcagggtg
    37861 attaagacga gacggctcgg atatgtaggg ttatcccttc tatcgcaatg acttcaacat
    37921 gcgtacctgc accgagatcc tcgctggcgc tgacaggcca tgaactgtca ccgacgcgca
    37981 tatgaccgcg cccgttgacc agcggagatt ccagcacaaa acgtcggcca atcagctgct
    38041 gcccgcgctg gtttaaatga ctatcgctgt gcttttgttc gcgcacccgc cgcgacaacc
    38101 atttccacca cagccaggcg gcgagcagcg tcaggatggc aaacattacc ccttgccact
    38161 cccaacccag cggcaccagc cagaccacca ggccggtaat caccgctgcc acgccgctcc
    38221 acaacaaata accatttccg cccagcatct cggctgccag cagcaacccg ccgagactga
    38281 gccagaaaat atgtggatga acgaccatta actccatcat ggctgagtcc gtttgttggc
    38341 gctgtctttc accagctcgg caatcccggc aatcgacccc atcaggctgc tggcctctaa
    38401 tggcatcatc actactttgc tgttactgga ggaaccgatc tgctgtaacg cttcggtgta
    38461 tttttgcgct acgaagtagt tcaccgcctg aatatcaccg gaggcgatag cttcagacac
    38521 cattttggtg gcgcgggctt ctgcttcggc ggaacgttca cgcgcttcag cctgtaaaaa
    38581 cgccgactga cgttcgcctt ccgctttcag gatttgcgac tgtttttcac cttcggcttt
    38641 gaggatttcc gcctgacgga tcccttccgc ttcaagaatg taagcgcgtt tggtacgttc
    38701 cgctttcatc tgcgcgttca ttgaagagat aagctctgcc ggtgggcgca cgtcgcgaat
    38761 ttcaatacgg gtgactttaa tcccccacgg gttggtggct tcatcgacaa tacgcagcag
    38821 gcgtgagttg atgctgtcgc gctgagagag catttcgtca agttccattg aacccaacac
    38881 ggtacggatg ttagtcatgg tcaggttgat gatcgccagc tccagattgc tgacttcata
    38941 ggcagcgcgt ggcgcgtcaa tcacctgaat aaaacagact gcgtcgatgg taacgttggc
    39001 gttatctttc gagataactt cctgggaagg gatatcgagc acttgctcca tcatattgat
    39061 cttgcgacca atgcgatcca taaacggcac caccagactg agccccggct gtaacgtttt
    39121 ggtatagcga ccaaaacgtt ctaccgtcca ttgatagccc tgcggcacga ttttgacgcc
    39181 cgcgccgaca atgaccagcg cgacaaaaat gagaatcggg ataaagataa gcatcggaaa
    39241 aacctcctgt tgtaccgtcc ataatcagca aaattgctgc ttgattaaac aaattatacc
    39301 tgattactga aagagagttc ccccttattc ctgcgaagga taaactgttt ttagtaaaaa
    39361 tcagaaaaag ggaacagcga tgcaggaaaa tagtcctttg cttcagctac aaaacgtagg
    39421 atatctggcg ggtgatacga agattcttaa taacatcaat ttttcgctgc gtgctggcga
    39481 atttaagtta attaccggtc cttctggttg tggcaaaagt acgctgctaa aaatagttgc
    39541 ttcattgatc agcccaacca gcggaacgtt actgtttgaa ggtgaggatg tcagcacact
    39601 aaaaccagaa atctaccgcc agcaagtctc ttactgcgcc cagacgccga cgctgtttgg
    39661 cgatacggta tacgataatc tgatctttcc ctggcagatc cgtgaccagc agcctgaccc
    39721 ggccattttt ctcgattttc tcgaacgctt cgctttgccg gacagcattt tgacgaagaa
    39781 tatcgccgag ctatctggtg gtgaaaaaca acgcatctca ttgattcgta acctgcaatt
    39841 tatgccgaag gttttattgc tggatgaaat aaccagtgcg ctggatgaaa gtaataaaca
    39901 taacgtcaat gagatgatcc atcgttatgt gcgcgagcaa aatattgccg tgctgtgggt
    39961 gacacacgat aaagacgaaa ttaatcatgc ggataaagtg attacactgc aaccgcatgc
    40021 cggagaaatg caggaagcac gctatgaact cgcataatat tactaacgaa tcattagcac
    40081 tggcattaat gctggtggtg gtggcaatct taattagcca taaagaaaaa ctggcgctgg
    40141 agaaagatat tctatggagc gtcgggcgag cgataattca gctgattatt gtcggctatg
    40201 tgctgaagta tattttcagc gtggatgatg ccagcctgac attattgatg gtgttattta
    40261 tctgctttaa tgcggcgtgg aacgcgcaaa aacgcagtaa atatattgct aaagctttta
    40321 tctcatcgtt tattgccatt acggtcgggg cgggaattac cctggcggtg ctgattctct
    40381 caggggcgat tgaatttatc ccgatgcagg tgatccctgt cgccgggatg attgccggta
    40441 acgccatggt agcggtgggg ttgtgttaca acaatttagg gcaacgggtc attagcgaac
    40501 agcaacagat tcaggagaaa ctgagtcttg gcgcgacgcc gaagcaggct tcagcgatat
    40561 tgattcgcga cagtattcgc gcggctttaa ttccgacggt cgattcagca aaaacggttg
    40621 gcttagtgag tttaccagga atgatgtccg ggctgatatt tgccgggatt gatccggtga
    40681 aggcgattaa atatcagatt atggtgacct ttatgctgct ctcaaccgcc agcttgtcga
    40741 ccattattgc ctgctattta acctatcgta agttttataa ttcgcgccac cagttggtgg
    40801 tgactcaatt gaagaagaaa tgatgcgatg ccggatgcgc agcatcgcat ccggcgttgt
    40861 ggttcatgtg ccgaacaacg gaatatcaat acaacaatgc atacagctgg cggcgatact
    40921 tcgacgccag tgcatcaccc gtacccagcg cagcgaggat ctcctggaac gttttacgcg
    40981 tctgaccgtc tgcggcggtg agatctttac gcagatgccc gaacagcaac tccagcgcct
    41041 cttcattgcg cccaacctga tgcagttgca gcgccagttg cgtcgccagt gcggcatctt
    41101 ctggattctc cgccacctgc tgttgcaact gttgaatttc cggcttatca gccgcctgct
    41161 tcagcagttc gatttgcgct accagcccct ggtagcgggt gtcctgatcc tgcaacggaa
    41221 tggttttcag caccgcttcc gcatcttcag aacggttcag cgcaatcagc gtttctgcca
    41281 gcagcaggcc gatctccccg ttctgattcg acaactgcca ggcgtctttc agcaatggca
    41341 gggcatcggt gtaattgcct tcctgcatca gttgcatcgc ctgctgcgct ttcagctctt
    41401 cttcgcgcgg cagcacttta tccagcaggg cgcggatcgc ctcttccggt tgcggcccct
    41461 ggaagccatc taccggttgc ccgttctgga acagatacac ggtcggaatc gcgcgcagac
    41521 caaactgggc ggcaatcatc tgctccgcgt cgcagtccag cttcgccaga ataaattgcc
    41581 cgttgtactg cgccgcgagg ctttccagga tcggggttaa ctgcaaacag tgctggctac
    41641 gttcagacca aaaatagaac agcaccggag tggtcatcga ctgttcaaga acctgttgca
    41701 ggttagattc gttaatgttg acaatatttt ctacggacat ggagtcgctc tctgttgtcg
    41761 atattttctt tgacatgggg gcttaagcgc gcgcttcaac tcacccctgc aatattttgt
    41821 ccatcacgcg cccaggcagc aggcgctcga gcaccattac tgcccaggtc accagcgtta
    41881 ccggatagcg catcttcggc ttctcgctaa taaaagcatg gcgcactttg tcgaccaccg
    41941 cttccggtcc caacgtaaag cgggcggcga tgccgggatt ttcgactggt ttatcacttt
    42001 gcgtctggtt gacgttgtcg gtgaagcgag tacgaatggg accgggttcg atcaggctga
    42061 ctttaattcc gctgtggcgt agctccatgc gcagcgcgtc tgaccacgcc tccagcgcat
    42121 atttactggc cgcgtaagcg ccacgacccg gcgtggagat taatcccatc accgatgatg
    42181 tcatcacaat acgcccttca ccgtgcggta acatcgcggg taacaggcgc atggtgagct
    42241 ggtgtgcgcc gaaaaagttg gcggaaaact gctgttccat ctgcgcacgg ctgatggtgg
    42301 aaagggggcc atacatgccg aatccggcat tgttaaagat cccatacaga caattatcgg
    42361 tcagggcgat cacctcgtct gctgcgcgat taacactttc tggtgaatcc agatcgatca
    42421 acacgccggt aaatcccatg ttgttcatac gctcaacatc attcggtttt cggcaacctg
    42481 ccagcacatg aaaaccctgg cgttttaatt cgagcgcgct ttccaggcca attccactgg
    42541 aacatccggt aattaagacc gatttttgca taactttacc tgtcaggatc tccgttgctt
    42601 tatgagtcat gatttactaa aggctgcaac tgcttcgcca tccagtcggc aataaacggc
    42661 tgggcgtcgc ggttgggatg aataccgtca tcctgcatcc attgtggctt gaggtagacc
    42721 tcttccataa aaaagggcag cagcggaaca tcaaactctt tggcgagttt ggggtaaatg
    42781 gcgctaaagg cttcattata acggcgacca tagtttgcag gcagacgtat ttgcattaac
    42841 aatggttcag cgttggcggc tttgacatcc tgcaaaatct ggcgcagcgt ttgctcggtt
    42901 tgctgtggct gaaaaccacg caaaccatca ttgcgcccag ttcaaccagc acccaacgcg
    42961 gctgatgctg tttgagcaga gccggaaggc gcgccagccc ttgttgcgag gtgtcgccgc
    43021 tgatgctggc attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc
    43081 aggccgcgct ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg
    43141 tgtccgctgc ggcggcacgg aaggttaaca ggaccaggaa caggaagggc aaatgccagc
    43201 ggaaaacatt gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact
    43261 ctccatcctc accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg
    43321 cgagtcggga tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag
    43381 cagtggcgaa gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc
    43441 aaagttgcgc gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct
    43501 taacgcgctg gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag
    43561 tcgtaacggg gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct
    43621 tccggcacag ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg
    43681 tcgacctgat gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga
    43741 taaaattgcc gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt
    43801 gacccacgac ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca
    43861 gttgcaggag gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta
    43921 ttaattgtct ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc
    43981 agcgatcgca tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg
    44041 gcgttgcgca gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc
    44101 ctgaaagtcg gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag
    44161 ctggcgaacg tcaaagcggt ggatgatatc tacccgatgt atggcggtct gcaaactaat
    44221 ccccctggcc tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg
    44281 cttaacctga aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga
    44341 gaagtgattc aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg
    44401 atgaatctgg cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg
    44461 cgttataaat tcggcggcaa cgagaaccag ctcgacggct atgagaaatg gttgttacct
    44521 cagcttaaac ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga
    44581 tcgatggaac gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca
    44641 gtggcagcgg tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg
    44701 atcctcaaaa cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg
    44761 ttgatggtgc tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac
    44821 gtgttgatgg tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg
    44881 ccgtggctgt gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca
    44941 taccgcttgt tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtcgcgaac
    45001 gtctggccgc tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc
    45061 ggattaatgg gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct
    45121 ttgctgtgcg gtgtgctggg ctggatgctg ctgaatgtac ttcgccgcat gacgctgaaa
    45181 tcgctgcctc tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc
    45241 cagctttcgg cattttcgct ctcctttatg ctgctggcac tgctgctggt gctgcgtggc
    45301 gatctgctcg accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt
    45361 aacatcgcca cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc
    45421 ccggaatcat tttatccggt agtgcgggcg cggctgacgg cgattaatga taagccgaca
    45481 gaaggtaatg aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg
    45541 cccgatcata atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg
    45601 gaagaggggc tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc
    45661 gatacccagg agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg
    45721 cggcctaatt tctattttat tttccctgaa ggggcattag acgggcaacc gcagagctgg
    45781 cttaccagtt tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc
    45841 ccgaccatta gcctgttaga cattggcgcg attttaaaac aggtcggtca ggtgctggag
    45901 caggtaagtc gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg
    45961 ttgctggcac aggtgcaggt gggaatgcgc cagcgtcatc aggagctggt ggtatggcgc
    46021 acactcggtg cggggaaaaa actgctgcgc acgacattgt ggtgtgatgt tcgccatgct
    46081 tgggtttgtt tccggcctgg tggccgcaat tggtgcggaa acggcactgg cagtgttgca
    46141 ggcgaaagtg tttgatttcc cgtgggagcc agactggcga ttgtggattg ttctgccgtg
    46201 cagcggtgcg ctgctgctgt cgcttttcgg cggctggctg ggtgcgcgac tggttaaggg
    46261 taaggcgctg ttcaggcagt ttgtggggtg atgacaggtg ttagattgcc tgatgcgcta
    46321 cgcttatcag gcctacgaag agcgtgcaat atattgattt tgcaagattg tgtaggccgg
    46381 ataaggcgtt tacgccgcat ctggcatgaa cagtgtgcat tttggcaata aatatgaaac
    46441 caactgtcat gtcggttttt ttaaaggccg aaggaggtaa accttcggcc ttagctacgt
    46501 ttcctcagtt attcaataaa ctgacggatc agttccagca gcgcgattag cgccttaaga
    46561 acgatgagaa ctttatcgag aaaagttttc atccgttgct cccttaaggg agaaagacaa
    46621 tcacagctta cccttacgtt gcctataact atgattttct tcatgtgcta gatgaggact
    46681 gtctctctcc aaattagagc accagaatca gcaccacctg tatcctgacc aggacttaaa
    46741 agtacttttc cgcactgcgg taatcccttg aggcagtatc actactagcg tcgggattgt
    46801 atcgccaaca tagctgtttg aatatgtagg catcctgact tataattcac ttgctagatg
    46861 aggactgttt aatttctgcc cggaaccacc aagtcttggg cggaaaaaat aaaaaaaccg
    46921 actgtcatgt cggttttttt atatctgtat tctggtgcaa ccattttgtt ctcacgacaa
    46981 tcgccgtttc agaataccgc ccatcactgc gccttaaccc ataccgccac ttccgcccac
    47041 tcaccgcgaa agacaacttt ttccgctttt ttctcaagct gatagcgata cattgggtcg
    47101 taatattctt taagtaacgg caccagccag gccagatgac cgtcggtgct gccggtggtg
    47161 agttgcgttg tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc
    47221 agccgacgct taatcgccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc
    47281 tgttcgtcgc cataagcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcattc
    47341 aggcgttcaa gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt
    47401 cgctcgcgca gacattccgg caggtgattc gaaccgatca tccggctttc atcttccagc
    47461 acccacaggc gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt
    47521 tcaaaactcg cctggctaag ttgtggttgt aacgtgcgac caaacgccga accgcgatga
    47581 cgcgccagcc cttccagatc aacaccgttt ggctgttgtt gcactaatag cgttttaccg
    47641 ctgccggtac aaccgccaat cagcactatc ggtttttgtg ccagttcaat ggtcgcctga
    47701 atcgcggtct ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct
    47761 tcatgcagcc agcgttgcac aatatgtgaa cgttgaccgc cacgggcgca gcagagaata
    47821 ccttgcggat tttgcaggca cgctgcccgc caggcgtaca tacgctgctg acgaatttca
    47881 cccgccacca gtttatgccc cagcgccagc gctgcgtctg agccttgctg tttatagcag
    47941 gtgccaacgg aggcgcgttc atcgttatcc attaacggca gattgatagc ggcgggcatt
    48001 gcgccttgct caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc
    48061 agggcacgat agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc
    48121 agcttgcgcg ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggg
    48181 tgccgaaaat ttctaaaaat ccgctgattt ccggcctgtg ttgggtaaac aaggtcacaa
    48241 tatcttctac cgctttgcag ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag
    48301 ggcgcatcgt tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa
    48361 gtgattttgg caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca
    48421 tatcgggaac aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg
    48481 tgccggcgct gtcttcaata ttgaccgccg gaaagcgacg caactgcgct tctgttagcg
    48541 gctcttcaac gttcgccagc ggatgatccg ccgccatgac aaatcgccat tgcaccgatc
    48601 ctaagggatc aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca
    48661 gcgaaaaacc ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt
    48721 gaaactgggt aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg
    48781 ggttatagag caggttgttg atgacaatat tcacctggcg ttccacgcca tcattcacct
    48841 gttgcagctc gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta
    48901 gatgctcgcc agccgctgtc aacgttacgc tacgagtcgt acggaaaaac agcgctactc
    48961 cggtattctc ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc
    49021 gttctgccgc ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt
    49081 ctggatcgaa catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc
    49141 ctctgattta tcagtatttt tacatgataa tcctgttcaa tttgtggact aaatctagtt
    49201 ttggaaaaac attccaactt ttgtattaat gttgttctct taaggtttta gattgcctgt
    49261 tattgaaacc aagctgaccg gtcggcggtg gttgaacgga attatgttac aaggacaaaa
    49321 agatgaaact tcaggtatta ccgttaagtc aggaagcctt tagtgcttat ggcgacgtaa
    49381 tcgaaacgca gcaacgggat tttttccata ttaacaatgg cctggtggag cgttaccacg
    49441 atttggcgct ggttgagatt cttgagcaag accgtacgct tatcagcatt aaccgcgcgc
    49501 aaccggcgaa tctgccgctg accattcacg aactcgaacg tcatccgctg ggcattcagg
    49561 cctttatccc aatgaaaggt gaagtgtttg tggtggtcgt ggcgttaggc gacgacaaac
    49621 cagacctgtc aacgctgcgg gcgtttatca ccaacggtga acagggggtg aattaccatc
    49681 gtaacgtctg gcatcaccca cttttcgcct ggcagcgcgt caccgatttt ctgaccatcg
    49741 atcgcggcgg cagtgacaac tgtgatgttg aaagtattcc tgaacaggaa ctctgttttg
    49801 cgtgacgcct gcaaccgact tgcataagat aaactaattg ttcattgttt atgctcactt
    49861 gtaggtcgga gttaacgtag gtatgacgga agttagacgg cgcggcaggc caggacaggc
    49921 ggagcctgtg gcacagaagg gcgcacaggc gttagagcgg ggaattgcga ttctgcaata
    49981 tctggaaaaa agtgggggaa gttcgtcggt tagcgatatt tctctcaatc tggatttgcc
    50041 gctctccacg acctttcgct tgctgaaggt tttacaagca gcggattttg tctatcagga
    50101 cagtcaatta ggctggtggc atataggatt aggtgtcttt aacgtcggtg cggcgtacat
    50161 ccataaccgc gatgtcctct ccgtcgccgg gccgtttatg cgccgcctga tgttactttc
    50221 cggcgaaacg gtcaatgtcg cgatccgtaa cggcaatgaa gcggtattaa ttggtcagtt
    50281 agagtgtaaa tcgatggtca ggatgtgtgc gccactgggc agtcgtctgc cgctgcatgc
    50341 ttccggtgcg ggcaaagcgc tgctttatcc gctggcggaa gaggagttga tgagcatcat
    50401 tctgcaaacc ggtttgcagc agtttacgcc aactacgctt gtggatatgc ccaccttgct
    50461 gaaggacctg gaacaagcgc gtgaactggg ctataccgta gataaagaag agcatgttgt
    50521 aggtctgaat tgcatagctt cagcaattta cgatgatgtc ggtagagttg ttgccgctat
    50581 ctccatctcc gggccttcat caagactgac agaagatcgt tttgtcagtc agggtgagct
    50641 ggtcagagac accgcccgcg atatcagcac ggcgttggga ctgaaagcac atccatagtg
    50701 tctgtcgcat cccgctctgc ggagcgggtt tttttgacaa aatttgaaag ttggaaaatt
    50761 tttccaacta atagaggtag gaataaaatg gcaaaaatga gagccgttga cgcggcaatg
    50821 tatgtgctgg agaaagaagg tatcaccacc gccttcggcg ttccgggagc tgcaattaat
    50881 ccgttctact cagcgatgcg taagcacggc ggtattcgtc acattctggc gcgtcatgtg
    50941 gaaggtgttt cgcacatggc ggaaggttat acccgcgcaa cggcagggaa tatcggcgta
    51001 tgtctgggga cttccggtcc tgcgggcacg gacatgatca ccgcgctcta ttccgcttct
    51061 gctgattcca ttcctattct gtgcattacc ggccaggcac cgcgcgcccg tctgcataaa
    51121 gaagattttc aggccgtaga tattgaagca attgctaaac cggtcagcaa aatggcggtt
    51181 acagttcgtg aagcggcgct ggtgcctcgc gtgctgcaac aggcatttca cctgatgcgt
    51241 tctggtcgtc cgggtccggt actggtggat ttaccgttcg acgttcaggt tgcggaaatc
    51301 gagtttgatc ctgacatgta cgaaccgctg ccggtctaca aacctgctgc cagccgtatg
    51361 cagatcgaaa aagctgtaga aatgttaatc caggccgaac gtccggtgat tgttgccggg
    51421 ggcggggtaa tcaatgctga cgcagctgca ctgttacaac agtttgctga actgaccagc
    51481 gttccggtga tcccaacgct gatgggctgg ggctgtatcc cggacgatca tgaactgatg
    51541 gccgggatgg tcggtctgca aaccgcgcat cgttacggta acgcaacgtt gctggcgtcc
    51601 gacatggtgt ttggtatcgg taaccgtttt gctaaccgtc ataccggttc ggtagagaaa
    51661 tacaccgaag ggcgcaaaat cgttcatatt gatattgagc cgacgcaaat tggtcgcgtg
    51721 ctgtgtccgg atctcggcat tgtctctgat gctaaagcgg cgctgacact gctggttgaa
    51781 gtggcgcagg agatgcaaaa agcgggtcgt ctgccgtgtc gtaaagaatg ggtcgccgac
    51841 tgccagcagc gtaaacgcac tttgctgcgc aaaacccact tcgacaacgt gccggtgaaa
    51901 ccgcagcgcg tgtatgaaga gatgaacaaa gcctttggtc gcgatgtttg ttatgtcacc
    51961 actattggtc tgtcacaaat cgctgcggca caaatgctgc atgtctttaa agaccgccac
    52021 tggatcaact gtggtcaggc tggtccgtta ggctggacga ttccggcggc gctggggggt
    52081 tgtgccgctg atccgaaacg caatgtggtg gcgatttctg gcgactttga cttccagttc
    52141 ctgattgaag agttagccgt tggcgcgcag ttcaacattc cgtacatcca tgtgctggtg
    52201 aataacgcct atctcggcct gattcgtcag tcacaacgcg cttttgacat ggactactgc
    52261 gtgcaactcg ctttcgagaa tatcaactcc agtgaagtga atggctacgg tgttgaccac
    52321 gtaaaagtag cggaaggttt aggttgtaaa gctattcggg tcttcaaacc ggaagatatt
    52381 gcgccagcct ttgaacaggc gaaagcctta atggcgcaat atcgggtacc ggtagtcgtg
    52441 gaagttattc tcgagcgtgt gaccaatatt tcgatgggca gcgaactgga taacgtcatg
    52501 gaatttgaag atatcgccga taacgcagcg gatgcaccga ctgaaacctg cttcatgcac
    52561 tatgaataag ggagataaat gatgttacgt ttctctgcta atttatcgat gttatttggt
    52621 gaatatgatt ttctcgcccg ttttgagaaa gctgcgcagt gtggttttcg cggcgttgaa
    52681 tttatgtttc cttatgacta cgacattgaa gaattaaaac aggtactggc gagtaataaa
    52741 ctcgaacata cgctgcacaa tttaccggca ggtgactggg cggcgggcga gcgcggtatt
    52801 gcctgtattc ctggccgtga agaagagttt cgggatggcg tagc
//
LOCUS       DAJTYH010000014        52616 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTYH010000014 DAJTYH010000000
VERSION     DAJTYH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..346
                     /locus_tag="OO609_000840"
     CDS             <1..346
                     /locus_tag="OO609_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6632357.1"
                     /translation="HHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELE
                     NSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQ
                     IMSRVKIVVMYN"
     gene            complement(384..1283)
                     /gene="nhaR"
                     /locus_tag="OO609_000841"
     CDS             complement(384..1283)
                     /gene="nhaR"
                     /locus_tag="OO609_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6632358.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1349..2515)
                     /gene="nhaA"
                     /locus_tag="OO609_000842"
     CDS             complement(1349..2515)
                     /gene="nhaA"
                     /locus_tag="OO609_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6632359.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3101..3253
                     /gene="mokC"
                     /locus_tag="OO609_000843"
     CDS             3101..3253
                     /gene="mokC"
                     /locus_tag="OO609_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6632360.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3357..4487)
                     /gene="dnaJ"
                     /locus_tag="OO609_000844"
     CDS             complement(3357..4487)
                     /gene="dnaJ"
                     /locus_tag="OO609_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6632361.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4576..6492)
                     /gene="dnaK"
                     /locus_tag="OO609_000845"
     CDS             complement(4576..6492)
                     /gene="dnaK"
                     /locus_tag="OO609_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6632362.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6869..7273
                     /locus_tag="OO609_000846"
     CDS             6869..7273
                     /locus_tag="OO609_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6632363.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7299..8012
                     /gene="msyB"
                     /locus_tag="OO609_000847"
     CDS             7299..8012
                     /gene="msyB"
                     /locus_tag="OO609_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6632364.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8161..8727
                     /gene="satP"
                     /locus_tag="OO609_000848"
     CDS             8161..8727
                     /gene="satP"
                     /locus_tag="OO609_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6632365.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8762..9349)
                     /gene="mog"
                     /locus_tag="OO609_000849"
     CDS             complement(8762..9349)
                     /gene="mog"
                     /locus_tag="OO609_000849"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6632366.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9464..10417)
                     /gene="tal"
                     /locus_tag="OO609_000850"
     CDS             complement(9464..10417)
                     /gene="tal"
                     /locus_tag="OO609_000850"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6632367.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10696..12126
                     /locus_tag="OO609_000851"
                     /pseudo
     CDS             10696..12126
                     /locus_tag="OO609_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12196..12972
                     /gene="yaaA"
                     /locus_tag="OO609_000852"
     CDS             12196..12972
                     /gene="yaaA"
                     /locus_tag="OO609_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6632368.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13125..13421)
                     /locus_tag="OO609_000853"
     CDS             complement(13125..13421)
                     /locus_tag="OO609_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6632369.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13635..14921)
                     /gene="thrC"
                     /locus_tag="OO609_000854"
     CDS             complement(13635..14921)
                     /gene="thrC"
                     /locus_tag="OO609_000854"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6632370.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14922..15854)
                     /gene="thrB"
                     /locus_tag="OO609_000855"
     CDS             complement(14922..15854)
                     /gene="thrB"
                     /locus_tag="OO609_000855"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6632371.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15856..18318)
                     /gene="thrA"
                     /locus_tag="OO609_000856"
     CDS             complement(15856..18318)
                     /gene="thrA"
                     /locus_tag="OO609_000856"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6632372.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18344..18463)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18399..18464)
                     /gene="thrL"
                     /locus_tag="OO609_000857"
     CDS             complement(18399..18464)
                     /gene="thrL"
                     /locus_tag="OO609_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6632373.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18678..19364)
                     /locus_tag="OO609_000858"
     CDS             complement(18678..19364)
                     /locus_tag="OO609_000858"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6632374.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19764..19904)
                     /locus_tag="OO609_000859"
     CDS             complement(19764..19904)
                     /locus_tag="OO609_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632375.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20000..20716
                     /gene="arcA"
                     /locus_tag="OO609_000860"
     CDS             20000..20716
                     /gene="arcA"
                     /locus_tag="OO609_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6632376.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20775..22127)
                     /gene="creD"
                     /locus_tag="OO609_000861"
     CDS             complement(20775..22127)
                     /gene="creD"
                     /locus_tag="OO609_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6632377.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22185..23609)
                     /gene="creC"
                     /locus_tag="OO609_000862"
     CDS             complement(22185..23609)
                     /gene="creC"
                     /locus_tag="OO609_000862"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6632378.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23609..24298)
                     /gene="creB"
                     /locus_tag="OO609_000863"
     CDS             complement(23609..24298)
                     /gene="creB"
                     /locus_tag="OO609_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6632379.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24311..24784)
                     /gene="creA"
                     /locus_tag="OO609_000864"
     CDS             complement(24311..24784)
                     /gene="creA"
                     /locus_tag="OO609_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6632380.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24995..25864
                     /gene="robA"
                     /locus_tag="OO609_000865"
     CDS             24995..25864
                     /gene="robA"
                     /locus_tag="OO609_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6632381.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25861..26508)
                     /gene="gpmB"
                     /locus_tag="OO609_000866"
     CDS             complement(25861..26508)
                     /gene="gpmB"
                     /locus_tag="OO609_000866"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6632382.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26560..27080
                     /gene="yjjX"
                     /locus_tag="OO609_000867"
                     /pseudo
     CDS             26560..27080
                     /gene="yjjX"
                     /locus_tag="OO609_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27165..27491)
                     /gene="trpR"
                     /locus_tag="OO609_000868"
     CDS             complement(27165..27491)
                     /gene="trpR"
                     /locus_tag="OO609_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6632383.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27581..29518)
                     /gene="sltY"
                     /locus_tag="OO609_000869"
     CDS             complement(27581..29518)
                     /gene="sltY"
                     /locus_tag="OO609_000869"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6632384.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29729..31396
                     /gene="ettA"
                     /locus_tag="OO609_000870"
     CDS             29729..31396
                     /gene="ettA"
                     /locus_tag="OO609_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6632385.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEPPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31503..31670)
                     /locus_tag="OO609_000871"
     CDS             complement(31503..31670)
                     /locus_tag="OO609_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632386.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31704..32936)
                     /gene="nadR"
                     /locus_tag="OO609_000872"
     CDS             complement(31704..32936)
                     /gene="nadR"
                     /locus_tag="OO609_000872"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6632387.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32957..34339)
                     /gene="radA"
                     /locus_tag="OO609_000873"
     CDS             complement(32957..34339)
                     /gene="radA"
                     /locus_tag="OO609_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6632388.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34388..35356)
                     /gene="serB"
                     /locus_tag="OO609_000874"
     CDS             complement(34388..35356)
                     /gene="serB"
                     /locus_tag="OO609_000874"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6632389.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35462..36106
                     /locus_tag="OO609_000875"
     CDS             35462..36106
                     /locus_tag="OO609_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6632390.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36134..37149
                     /gene="lplA"
                     /locus_tag="OO609_000876"
                     /pseudo
     CDS             36134..37149
                     /gene="lplA"
                     /locus_tag="OO609_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37181..37444
                     /locus_tag="OO609_000877"
     CDS             37181..37444
                     /locus_tag="OO609_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6632391.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37605..38324)
                     /gene="deoD"
                     /locus_tag="OO609_000878"
     CDS             complement(37605..38324)
                     /gene="deoD"
                     /locus_tag="OO609_000878"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6632392.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38381..39604)
                     /gene="deoB"
                     /locus_tag="OO609_000879"
     CDS             complement(38381..39604)
                     /gene="deoB"
                     /locus_tag="OO609_000879"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6632393.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39656..40978)
                     /gene="deoA"
                     /locus_tag="OO609_000880"
     CDS             complement(39656..40978)
                     /gene="deoA"
                     /locus_tag="OO609_000880"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6632394.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41105..41884)
                     /gene="deoC"
                     /locus_tag="OO609_000881"
     CDS             complement(41105..41884)
                     /gene="deoC"
                     /locus_tag="OO609_000881"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6632395.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42142..43683
                     /locus_tag="OO609_000882"
     CDS             42142..43683
                     /locus_tag="OO609_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6632396.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43655..44518
                     /locus_tag="OO609_000883"
     CDS             43655..44518
                     /locus_tag="OO609_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6632397.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44732..45514)
                     /locus_tag="OO609_000884"
     CDS             complement(44732..45514)
                     /locus_tag="OO609_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6632398.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45511..46584)
                     /locus_tag="OO609_000885"
     CDS             complement(45511..46584)
                     /locus_tag="OO609_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6632399.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46706..46867)
                     /locus_tag="OO609_000886"
     CDS             complement(46706..46867)
                     /locus_tag="OO609_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6632400.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46984..47589)
                     /gene="osmY"
                     /locus_tag="OO609_000887"
     CDS             complement(46984..47589)
                     /gene="osmY"
                     /locus_tag="OO609_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6632401.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47982..49571)
                     /gene="prfC"
                     /locus_tag="OO609_000888"
     CDS             complement(47982..49571)
                     /gene="prfC"
                     /locus_tag="OO609_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6632402.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49662..50339)
                     /gene="yjjG"
                     /locus_tag="OO609_000889"
     CDS             complement(49662..50339)
                     /gene="yjjG"
                     /locus_tag="OO609_000889"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6632403.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50354..50800)
                     /gene="rimI"
                     /locus_tag="OO609_000890"
     CDS             complement(50354..50800)
                     /gene="rimI"
                     /locus_tag="OO609_000890"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6632404.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50769..51182)
                     /gene="holD"
                     /locus_tag="OO609_000891"
     CDS             complement(50769..51182)
                     /gene="holD"
                     /locus_tag="OO609_000891"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6632405.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51285..52316
                     /gene="rsmC"
                     /locus_tag="OO609_000892"
     CDS             51285..52316
                     /gene="rsmC"
                     /locus_tag="OO609_000892"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6632406.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12530 a        14237 c        13191 g        12658 t
ORIGIN      
        1 tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt ttgaacccag
       61 tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca gcatattaaa
      121 aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac tctccaatga
      181 tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc atccccatga
      241 aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca atgtctcaag
      301 cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta tctcccccac
      361 cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc agaataatcc
      421 gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa aatagcatgg
      481 tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc ggcataaaag
      541 tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc aaaagctttc
      601 atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg ggagttaaac
      661 cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag tcgccgttct
      721 tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa actcacgcca
      781 cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat cggacagtca
      841 gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc gtgggtggat
      901 tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag tacgctactg
      961 accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt ggattctttg
     1021 cgatagttca caatatccag catttcctgg cttaaggtga acattttatc ggcatagcga
     1081 tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt aaataatttg
     1141 ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg agttaaataa
     1201 agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa gtaatacaag
     1261 tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga aaagggagcc
     1321 gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga acgcgtaacc
     1381 agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt ttcgcccagt
     1441 taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata gacatagtaa
     1501 aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt ccctcaggca
     1561 gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc agcggtttgc
     1621 caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca tccagcgtga
     1681 cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc agatacgcca
     1741 cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc ttctctttca
     1801 aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc gatttcaaca
     1861 ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt acaccacaca
     1921 gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag gccatcgata
     1981 agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg ataatagcca
     2041 gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc gccagtacac
     2101 caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg gtaatcggat
     2161 cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca atagcggcga
     2221 taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt tcacgtttaa
     2281 cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc cataacagca
     2341 tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc agaaagtcgt
     2401 gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata gcggcaatga
     2461 taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt ttcacttttt
     2521 atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa tcatcgttaa
     2581 aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa tttgcgccat
     2641 cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta agcccagata
     2701 atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata attaatttgt
     2761 tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt gtcgtcaata
     2821 attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat tacgccacaa
     2881 aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa gagagcgata
     2941 taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg cagatagaga
     3001 aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga acacatgtag
     3061 agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc ataaggcgat
     3121 gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg taacgagaaa
     3181 agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt tcacggctta
     3241 cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg tgctcgcata
     3301 tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg gggaggttag
     3361 cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga gcgcgggctg
     3421 ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag ctgtttctgc
     3481 ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc accctgtgcg
     3541 ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc ggtctgggtt
     3601 tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat ttcgccaccc
     3661 agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc ttcacgctcg
     3721 aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg tgcgccatgc
     3781 tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac ccctgccggg
     3841 attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca tttgttgcac
     3901 ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg tacagcgaag
     3961 aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt ctgcggctgt
     4021 gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt cggaatgcgg
     4081 atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt atagcgtaaa
     4141 tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc gccgaaaacg
     4201 tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc catgcctcct
     4261 tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga gtcggtcaga
     4321 acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc accctggtta
     4381 cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc acgctcttcc
     4441 gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt ttttaaattg
     4501 cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca gtataattac
     4561 ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt cgacaacatc
     4621 gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct gggcatgttg
     4681 ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca ttttcgcttc
     4741 gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg cagactcgat
     4801 agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct tacgggtgct
     4861 gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact tacggtcagc
     4921 ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt tcagaccaga
     4981 agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg cggaaacgtg
     5041 caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc cgcgcggtgc
     5101 cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg cacgtttacg
     5161 ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg tagagaacac
     5221 ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca tcacaccgcc
     5281 catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt cttttacgtc
     5341 accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt ccgggttaac
     5401 gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca ttggcatacg
     5461 agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc cagcatcctg
     5521 cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca ggctttccag
     5581 tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg cagtgatgta
     5641 tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt tttctgccgc
     5701 ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct gatctttctt
     5761 gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac ccaggtgggt
     5821 atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt cgatttcgat
     5881 aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac ggttgccagt
     5941 gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga tacgttttac
     6001 ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat cgttaaagta
     6061 tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt cagcggtttt
     6121 cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct ggcctttaac
     6181 ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga tggaaacatc
     6241 acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg caaacagggt
     6301 gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag tttcaccatc
     6361 ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct ccagcacgcg
     6421 aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt cgataccaat
     6481 tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt gtgagtctgt
     6541 aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag actcactcac
     6601 tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat gcaaaaattt
     6661 tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa ttgacagcaa
     6721 ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt gtaataaaaa
     6781 gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg tattcatccc
     6841 cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt ccgccagcct
     6901 ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac tcctcggcgt
     6961 cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg tttgtcgcat
     7021 tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg gtgcatcggt
     7081 ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag aaataaaaga
     7141 agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac gctgcgtctc
     7201 aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc tgaaaattat
     7261 cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat tacctgaatg
     7321 attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat ttcgcccgat
     7381 tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg cgcaacgaac
     7441 tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag ctgattgccg
     7501 gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat
     7561 tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc gacaaagaga
     7621 tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga
     7681 aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc
     7741 tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt gtgtcgcatt
     7801 tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc gtacttgggc
     7861 atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta aatggggtta
     7921 aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa atcgcctgcc
     7981 tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa attttcattt
     8041 tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa
     8101 cactacagtt attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt
     8161 atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc
     8221 accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat tattcttgcc
     8281 atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga gtacaaaaaa
     8341 ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt
     8401 gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca gttccttggt
     8461 gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac tctgaaaggc
     8521 gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc
     8581 ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg tctgatctgc
     8641 ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt tggtcgcacc
     8701 gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg gggggatttt
     8761 tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc acttctggtg
     8821 ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac
     8881 catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct ttaatagact
     8941 tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc acctgacgcg
     9001 aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagccag
     9061 gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgccgggc
     9121 cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc tcacacaacg
     9181 tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca aacggcgtgg
     9241 ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga taaacgccgc
     9301 tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc
     9361 cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggcttc
     9421 agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc agatcgccga
     9481 tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc gccagtttat
     9541 ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg atacgcgccg
     9601 gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct tcgctctccg
     9661 ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc agttccagaa
     9721 tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg tgctctttgt
     9781 agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga gcgtactctt
     9841 tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc gagatcagga
     9901 acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg gtcaggttac
     9961 agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag gtagaagcca
    10021 gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg atcaggcgtt
    10081 ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact tcagttgaga
    10141 tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt ttgtcggtcg
    10201 cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat
    10261 caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg
    10321 tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg gccactacgg
    10381 tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc
    10441 ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc
    10501 gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact gcgttgatat
    10561 cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca agtacctcgt
    10621 agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca
    10681 ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg
    10741 gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac cggatttgtg
    10801 cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca
    10861 ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt gggtagcggc
    10921 aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt cttttggatg
    10981 tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct tgcacaactt
    11041 tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc
    11101 gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat cgcctacggc
    11161 ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt ttcttttgat
    11221 tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc aatcactcgt
    11281 ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc gataatctgg
    11341 gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca cgtcatttgg
    11401 tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc
    11461 ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga ggtgggaatg
    11521 ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc ggcagcacaa
    11581 gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc
    11641 atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt
    11701 atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt taccctcgtg
    11761 gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc
    11821 ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg caccttcgca
    11881 acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc agatatcata
    11941 atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc tgtggttcat
    12001 accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg
    12061 ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga tgtttcgcag
    12121 gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc
    12181 ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga ttaccaaagc
    12241 ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca gcagttgatc
    12301 catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat cagcgacaaa
    12361 ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggcgaat
    12421 gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc
    12481 ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat
    12541 ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt
    12601 gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg
    12661 aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat
    12721 tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat
    12781 gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg cggtctgatg
    12841 agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc
    12901 gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt taaacgctac
    12961 gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat ccggcctaca
    13021 aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac gcgccagcgt
    13081 cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg gtgatgtttg
    13141 cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg cggcggtggc
    13201 ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt ccaccagccg
    13261 tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc gcctatctgt
    13321 aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg agcgaccaga
    13381 accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc cttttatcat
    13441 tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg ttattctccg
    13501 caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc ctgtcgtttt
    13561 tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg cctgattgag
    13621 ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga aaaatcggcg
    13681 ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt tggcagatcc
    13741 aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg cgcggtgccg
    13801 aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata cgctacggca
    13861 gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg ttgcgtggtt
    13921 tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg gcggaacaac
    13981 tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc
    14041 tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt atcgttcacg
    14101 ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag cagacccgcc
    14161 gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg cgcctcctgc
    14221 ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg gctgatgtta
    14281 atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc aaacgcctgc
    14341 ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc gatattgccg
    14401 cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg atagaggata
    14461 accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc ggtatcaccg
    14521 gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag catttgtgcc
    14581 ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa ttccagacaa
    14641 ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc gcgcacgcgc
    14701 tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat cttcgcactg
    14761 cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa ttccggcagg
    14821 tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc ttgcgcaaag
    14881 ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc cagtactcgt
    14941 gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg caggtagttc
    15001 ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag agcgaacaag
    15061 gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac cgcctgccgc
    15121 gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac atctttcatc
    15181 agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc agccagatgt
    15241 cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc ccgggcttct
    15301 gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc aaaccctggc
    15361 acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc aagaaaacac
    15421 ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc gcccatcaaa
    15481 gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt cattcatcgc catcagcgcc
    15541 gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt cttttccaga
    15601 gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca gcactgataa
    15661 acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag tttgttgaga
    15721 ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc atcaacgggt
    15781 gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga agccggggca
    15841 taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca gatcggcaaa
    15901 gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca acggcagcgg
    15961 ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt tgaacagcgg
    16021 atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat caatattgcc
    16081 aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc
    16141 gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt taaactctgc
    16201 gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg tttcacgagc
    16261 gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg ggtccggttc
    16321 ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc cttcgtctaa
    16381 cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca ccaattcatc
    16441 acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc caacgttggt
    16501 gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat gatagtaatc
    16561 catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt cgcgcaggaa
    16621 gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga ccgggttcag
    16681 cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg
    16741 tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga gcagagcctt
    16801 cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc agctttgctg
    16861 acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca caaacacttc
    16921 gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag tggtcgcatc
    16981 atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa tggcgacaat
    17041 gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg
    17101 cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca gcggctccag
    17161 taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag ctcgcacaca
    17221 gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg taatcagcac
    17281 cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc caaccatccc
    17341 tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg aaatgccctt
    17401 gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg cttgaggatt
    17461 tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa tggtgcgggg
    17521 gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg acatcgactt
    17581 caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt caacgtccgt
    17641 ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg agtagtcgga
    17701 accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga aacctgccat
    17761 caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg actcagcaat
    17821 atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg gatcgataac
    17881 ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg acattttctc
    17941 gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca aactaatgcc
    18001 atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca attgcgccag
    18061 cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa tacgttcggc
    18121 atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca tcgccaccag
    18181 gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct gcctggcatt
    18241 gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg atgtaccgcc
    18301 gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa aagcccgcac
    18361 tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg
    18421 gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct
    18481 gtaattttta tctgtttgtg cgctatgcct atattggtta aagtatttag tgacctaagt
    18541 caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta tcagacactc
    18601 tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat
    18661 acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc
    18721 aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca gtcgaccagt
    18781 tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc gcgtaaagct
    18841 tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat taatgttgct
    18901 aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata atccgccacc
    18961 atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag cccggaatct
    19021 tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc
    19081 accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc agtggtggcg
    19141 acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt aatattatca
    19201 ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg tgcctgacta
    19261 tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc tgccccaata
    19321 ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc actcttcttg
    19381 accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg aatttacaaa
    19441 ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct aaactatttt
    19501 ctgactgcac taacggttta attgttaaaa aatgctacat atccttctgt ttacttagga
    19561 taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag ttgtatgatg
    19621 caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca
    19681 tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg
    19741 ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt
    19801 tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg
    19861 ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt
    19921 taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt
    19981 ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt
    20041 aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac
    20101 agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga
    20161 tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa
    20221 tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga
    20281 aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc
    20341 acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt
    20401 tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc
    20461 tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga
    20521 aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg gccgtgagct
    20581 gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc
    20641 tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc gcttctgcgg
    20701 tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt tagcgccgtt
    20761 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
    20821 gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac aaacatcatg
    20881 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc ggcagagttg
    20941 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
    21001 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
    21061 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
    21121 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
    21181 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
    21241 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
    21301 gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
    21361 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg tttggctggt
    21421 agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    21481 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc catattcagc
    21541 tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    21601 tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc aggcgctttc
    21661 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt gcctaagatg
    21721 atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    21781 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    21841 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    21901 gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    21961 acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    22021 tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa cagcaatact
    22081 gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt
    22141 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    22201 tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    22261 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    22321 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    22381 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
    22441 gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    22501 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac atgcaaagtg
    22561 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa taatgccgcc
    22621 acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    22681 gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt aaaacgagcc
    22741 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    22801 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    22861 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
    22921 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
    22981 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat ccccaacaaa
    23041 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
    23101 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat cggcgcggca
    23161 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg cgcaccatac
    23221 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc aacggcttta
    23281 tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg atattcattg
    23341 cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag ctgattaaac
    23401 gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    23461 gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac gcccggttta
    23521 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    23581 cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat cccatgccgc
    23641 gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca cgcagcgttt
    23701 taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    23761 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    23821 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    23881 tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc ttcacccgac
    23941 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
    24001 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    24061 acagtacagg taacgccgga tagagcgcca gtaactggcg gcacaattca aagccactga
    24121 tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
    24181 gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    24241 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    24301 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
    24361 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    24421 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    24481 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    24541 aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta ataccaccgg
    24601 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    24661 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
    24721 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    24781 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    24841 attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa catactctac
    24901 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    24961 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    25021 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    25081 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    25141 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    25201 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    25261 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    25321 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
    25381 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    25441 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
    25501 caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    25561 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
    25621 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    25681 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
    25741 aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    25801 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
    25861 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    25921 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    25981 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    26041 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
    26101 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    26161 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    26221 cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc
    26281 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    26341 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    26401 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    26461 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    26521 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg
    26581 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    26641 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca accctttggc
    26701 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    26761 gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac tttcagttgg
    26821 gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt gccgttacct
    26881 gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag tcgcgttata
    26941 ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac
    27001 tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcacaatg
    27061 ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac attcggtgca
    27121 cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc
    27181 aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct gttagatcca
    27241 cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg actcatttcg
    27301 ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc
    27361 gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg
    27421 tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat
    27481 tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc ccccggttgt
    27541 actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt
    27601 ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcatcataag
    27661 ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca
    27721 cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt
    27781 tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt
    27841 aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg ctgctataac
    27901 caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc
    27961 cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg
    28021 ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa agatcgttgt
    28081 aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa
    28141 cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt
    28201 ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg agattccagt
    28261 acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg tcaacattct
    28321 gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct gcaaccatcg
    28381 ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca gcttcacgtc
    28441 cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca
    28501 tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca agcgccattc
    28561 gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt
    28621 tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata tcgcgcagct
    28681 cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg
    28741 cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa
    28801 aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg
    28861 aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta
    28921 ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat
    28981 cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct
    29041 tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc
    29101 atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg
    29161 ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg
    29221 tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt
    29281 gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc ggataatcct
    29341 tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag gcctgcttga
    29401 tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg
    29461 tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa
    29521 gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct
    29581 aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg
    29641 atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa aactcatcat
    29701 cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt
    29761 cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc
    29821 tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc tgctgcgcat
    29881 tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat
    29941 tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga
    30001 agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta
    30061 cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat
    30121 cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct
    30181 gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt
    30241 ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa
    30301 ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact tcgaaggcac
    30361 cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga
    30421 actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc tggagcagaa
    30481 agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa
    30541 agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct
    30601 ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa ccaacgaact
    30661 gtttattcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa
    30721 atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcgat
    30781 cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctccgg
    30841 tcaggaaccg ccggacagcg gcaccatcac tttgggtgaa acggtgaaac tggcatcggt
    30901 tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg
    30961 gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt
    31021 taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg gtgagcgcgg
    31081 tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc
    31141 aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc
    31201 gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat
    31261 cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga
    31321 agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta tcaagtacaa
    31381 gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta tccggcctac
    31441 aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg gtaagcgtcg
    31501 catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg gtctacaaca
    31561 gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa accttgttta
    31621 atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat tttatacctc
    31681 gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg caccagctcc
    31741 acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa ttcgatattg
    31801 ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg
    31861 aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg
    31921 aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc gtactttttg
    31981 cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt ggcgtatttc
    32041 actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc ggaatactgc
    32101 aatgcgattt catcaccgcc gaggtgtgaa aagacataat cgcggccata ttcccacgca
    32161 ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg
    32221 ccgccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg aatatattcc
    32281 cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc
    32341 ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc atcggcttct
    32401 tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt tttgatgccg
    32461 ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt gaaagcatga
    32521 atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg atccggcacc
    32581 gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa
    32641 cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt
    32701 ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgccgggga
    32761 aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg gcttttaatt
    32821 ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc
    32881 tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct
    32941 ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa
    33001 cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg
    33061 cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg ggcaccgggc
    33121 ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt
    33181 cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt tcggttacct
    33241 tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga ccaccgtgac
    33301 ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc
    33361 gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg
    33421 ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga
    33481 aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca
    33541 gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa cgggagtcgg
    33601 catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc gggccagcca
    33661 gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc gttttggcga
    33721 aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta
    33781 catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt tgctcttctt
    33841 cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga ttgtcagtcg
    33901 gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccggtgacat
    33961 acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc gtggatttcc
    34021 ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg cctcctagta
    34081 cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg
    34141 agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac
    34201 gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg
    34261 cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc
    34321 gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatgacggg
    34381 caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag
    34441 cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc
    34501 ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct
    34561 gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct ttgtactgcg
    34621 cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc tccagttcat
    34681 tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag aaagtaaagc
    34741 cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc agctgcgtta
    34801 agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt
    34861 tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc
    34921 gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt tcatcaatac
    34981 attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt
    35041 gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg cgtgtagccc
    35101 gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa
    35161 caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc
    35221 cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat
    35281 caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc
    35341 aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg
    35401 aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa
    35461 gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc tgttctgtct
    35521 tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca
    35581 gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt
    35641 tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga tcctcgatca
    35701 gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat
    35761 cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg gtaaaaaagc
    35821 aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg
    35881 ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa
    35941 caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac
    36001 ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc
    36061 cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag
    36121 aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt
    36181 taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt
    36241 tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt ggaaagagtg
    36301 taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg gcggtggcgg
    36361 cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta
    36421 cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc
    36481 cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc gcaaagtctc
    36541 aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct tgctactcaa
    36601 tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa
    36661 aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggctcac
    36721 catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg cgagcacgtg
    36781 gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt
    36841 gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc gcatctgctg
    36901 gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc
    36961 acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg
    37021 cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg
    37081 gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg
    37141 gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc tggaaaacca
    37201 ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag ccgccgtgcg
    37261 tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc
    37321 tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca aaatggcaga
    37381 aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa
    37441 atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat atgaaaaata
    37501 atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtcc
    37561 cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc
    37621 agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg
    37681 gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt cagggctttc
    37741 gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac gccgaggatg
    37801 ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag
    37861 aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac tgcgttacgc
    37921 accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat gcggttaact
    37981 ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac gtgcggcaga
    38041 actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt gatcagttct
    38101 ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg
    38161 cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct
    38221 tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg
    38281 tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt atcctttttg
    38341 tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg gctttgccat
    38401 attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc gcgaaggttt
    38461 cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc ggaatgtgtt
    38521 cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg gtgaggatca
    38581 ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg aacagttcca
    38641 gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg aagtcaacga
    38701 agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg gtggcgtcaa
    38761 acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg gcgtagatgt
    38821 ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt ttctgcagca
    38881 cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc tggaagttac
    38941 cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag ccgccgttgg
    39001 tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg aaagtttctt
    39061 catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc ttgccggttt
    39121 tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg cagttaccga
    39181 ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc gggaagctgt
    39241 tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg atttcccagt
    39301 gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac gcgccgataa
    39361 cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg tgtgctttcg
    39421 ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca ttatcagctt
    39481 cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg tcaccaaagc
    39541 gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata aatgcacgtt
    39601 tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta taccgttatt
    39661 cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca agtttaattg
    39721 ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg aggataaccg
    39781 ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta aagccgacgc
    39841 tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact actgccatcc
    39901 ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag actgctttcg
    39961 tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc gggccttttt
    40021 gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc accgcctgca
    40081 atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac atctccacgc
    40141 acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc aggaactgca
    40201 ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga ttcatgtcgg
    40261 tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc gcttcggcaa
    40321 gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc acgtccatca
    40381 ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg atcagcggga
    40441 tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca gccggagcca
    40501 gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg cggaaacggt
    40561 tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc gtaccgccag
    40621 tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct gcgaccatcg
    40681 gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta tcaacaatcg
    40741 ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc atcgccatgg
    40801 tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc gcgagggcgg
    40861 caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa cgaatttctt
    40921 catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg agaaacaagg
    40981 taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac gccttatccg
    41041 gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc gtaaagcatc
    41101 ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc agcaggcttg
    41161 ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca gcaccgaaca
    41221 gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg ccgcccgccg
    41281 gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc atgatgcgag
    41341 cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg aagtccgcac
    41401 ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc agttcgccgg
    41461 tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta caggctttca
    41521 ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg aacaccacgt
    41581 caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg atttcgatgt
    41641 cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg gtgccctgct
    41701 ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata gcggcggtat
    41761 tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg gtgtcgtcgt
    41821 cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct ttcagatcag
    41881 tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg ttagtattct
    41941 aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct acttacaagt
    42001 ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg
    42061 tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg cgcatcacct
    42121 tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca acaacgttgc
    42181 cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt tctggcactg
    42241 gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg cgcgctggat
    42301 gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta
    42361 cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga aggcgcgaaa
    42421 gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc gtcggtcaca
    42481 tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt tagaattcta
    42541 acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga cagaaccctg
    42601 ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac ccgcgcgatc
    42661 ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc tgaaatcacc
    42721 cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa accgcacatc
    42781 gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat tgtgagctgt
    42841 tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa cctgaaagcc
    42901 attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc gcactactgc
    42961 cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca atcacacttc
    43021 tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt tgtgccaatg
    43081 tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga agggattgcc
    43141 gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag cgcgcaactg
    43201 gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc catgttacac
    43261 gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt gccgtatggc
    43321 gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc ttattattat
    43381 tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc gcaggcactg
    43441 gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc caatgtcagc
    43501 ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt agaaaaatat
    43561 cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc acgcaacact
    43621 cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg ctttagtcag
    43681 taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc tgtttttgca
    43741 gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac gttgcaatga
    43801 ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg acggcaaagt
    43861 ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt gtccgcaaca
    43921 tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg tgcgcaaagc
    43981 ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc aactgccgtt
    44041 tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc tcacctgtct
    44101 ggtggacagt aacggtatgt tgagcgaaac cggctgggaa aaattactcc cggtgtgtga
    44161 cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat taaccggacg
    44221 cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca agctggcgga
    44281 actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg aagaactggc
    44341 ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc atgcccacgg
    44401 cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg agccgttggc
    44461 tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct atttgtgaca
    44521 tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg cgtcaagcgt
    44581 cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc cggcctacgg
    44641 ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg cattatgcgc
    44701 cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac accgcatacg
    44761 tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac tcgcaaagca
    44821 cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa ccgttgagcg
    44881 gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt gcgatgacat
    44941 cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt ttgtagccca
    45001 gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca acaccagcac
    45061 gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc cgtgaatgca
    45121 ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt aaccactgct
    45181 gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc accgccacca
    45241 ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca tcgctatgtt
    45301 tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa ttttccgcta
    45361 acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc ttgcctacgc
    45421 ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc gggaaatcaa
    45481 aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt taaatgtcgg
    45541 gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg cgactggcgc
    45601 aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg taagcggcgc
    45661 tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata aacgcccaag
    45721 tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat gtaatggctt
    45781 cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa attgctgaat
    45841 gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca ggctactgtc
    45901 acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt gtgacggcac
    45961 agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa ccggaatcgc
    46021 atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc tacgataaaa
    46081 gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg ttggtaaaaa
    46141 gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt tgccgctgtc
    46201 aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg cctccaccag
    46261 ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga aaaattcgcg
    46321 ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc agataaacgc
    46381 cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat taaactgcgc
    46441 gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc cgccttcgca
    46501 caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa tattaccaag
    46561 cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa agaaagacaa
    46621 agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac tggctttgga
    46681 taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac aaactcacta
    46741 ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg gtaccggcca
    46801 gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg atgccccaac
    46861 gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag cgtgaaaatc
    46921 actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt tcgggaggac
    46981 gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg ctttggcgat
    47041 actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct gaaccacgcc
    47101 atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca gcagtttggc
    47161 tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc cttctttagc
    47221 gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt tcgccacttt
    47281 cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca cgactttttg
    47341 atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca gcgccgcctt
    47401 cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca tagagctatc
    47461 gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt aggcagagcc
    47521 ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct tcagtcttgt
    47581 catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca acacgaggtt
    47641 gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc catcacaaaa
    47701 gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat atcggtagaa
    47761 tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta acgccatcag
    47821 aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg atatcagcga
    47881 aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata aggaagggaa
    47941 attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc gcgggtctgg
    48001 tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat gctggtagcg
    48061 atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt acgcttgaac
    48121 tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac gttgactgac
    48181 tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac atcaaactgc
    48241 agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa cacctgcacc
    48301 gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt cagcggatct
    48361 ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt gaacttcatc
    48421 atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg caggccgagg
    48481 atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa ggtcagcgcg
    48541 tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc tttttcatat
    48601 ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc catgttggcc
    48661 tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt acgggtatcg
    48721 gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat atgatcaacg
    48781 ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc aaggaacagc
    48841 tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg cagctgctgt
    48901 gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt aacaatgcgg
    48961 acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc tttataaagg
    49021 tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg tgcgcagcca
    49081 attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat atcacggtca
    49141 agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc catcagctta
    49201 cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca gtccaccgcc
    49261 gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc gagcaggtta
    49321 accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat cccacgctgc
    49381 ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc ttttactgta
    49441 ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt agttttaccg
    49501 gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt caaataagga
    49561 gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt gttgaaaacg
    49621 aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt acacaggagc
    49681 tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc tggttgctcg
    49741 cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc gagaatatcg
    49801 gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc cgcctgttcc
    49861 agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc ggaaatcacc
    49921 agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg caaggcgcta
    49981 aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag cagagaaacc
    50041 gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc gttaagcaag
    50101 cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg aagctgtaat
    50161 gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc ctgatagtcc
    50221 tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg caggccggtg
    50281 aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc ccacttcatt
    50341 attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct tcgcgaccgt
    50401 ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa ctttcgtaca
    50461 gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc acgccgcgtt
    50521 tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga cgctgatagt
    50581 cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc gtaatcgcaa
    50641 acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg ccctggttgc
    50701 tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg tggtaagccg
    50761 ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat ttgttgccat
    50821 aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga tgccacctga
    50881 gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca gcgactgcct
    50941 tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg gctgacggtt
    51001 aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc gtttgccacc
    51061 atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa cgcgccaggg
    51121 cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg tcgggatgtc
    51181 atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga taccgcgcca
    51241 acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct gcatttaccc
    51301 cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt attctgtttg
    51361 ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc cgtgctcata
    51421 cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac gcccgtttta
    51481 gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac tactggccga
    51541 agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg ccagtgggga
    51601 cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag cagatgctgg
    51661 cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc tattttggtc
    51721 gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac agcgtcgatg
    51781 gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat gtcggtagcc
    51841 agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat gtcggctgtg
    51901 gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt ctcaccttgt
    51961 gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc aacggtgttg
    52021 aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt gatatgatca
    52081 tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg caaacgctga
    52141 ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta gcgaacgcct
    52201 tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt atcgcgcaaa
    52261 ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa ggttgagtat
    52321 ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc cgacctacaa
    52381 aacctgcacg ttagcccttc gtaggccaga taagacgcgc cagcgtcgca tctggcatct
    52441 gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt tccagcaatt
    52501 acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa tgcgcctccg
    52561 tggtagcaat tctttttaag aattgatggt atgcgaaggt ggcggaattg gtagac
//
LOCUS       DAJTYH010000015        49532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTYH010000015 DAJTYH010000000
VERSION     DAJTYH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1061
                     /locus_tag="OO609_000893"
     CDS             <1..1061
                     /locus_tag="OO609_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6632407.1"
                     /translation="IVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNAT
                     YMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPG
                     GNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIE
                     LVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLT
                     DKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAV
                     PETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQ
                     AEKYLLAQFGVDIGEAAQ"
     gene            1058..2023
                     /gene="yggF"
                     /locus_tag="OO609_000894"
     CDS             1058..2023
                     /gene="yggF"
                     /locus_tag="OO609_000894"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6632408.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2045..2554
                     /gene="fumE"
                     /locus_tag="OO609_000895"
     CDS             2045..2554
                     /gene="fumE"
                     /locus_tag="OO609_000895"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6632409.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2551..3264
                     /locus_tag="OO609_000896"
     CDS             2551..3264
                     /locus_tag="OO609_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6632410.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3236..3913)
                     /locus_tag="OO609_000897"
     CDS             complement(3236..3913)
                     /locus_tag="OO609_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6632411.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3907..4584)
                     /locus_tag="OO609_000898"
     CDS             complement(3907..4584)
                     /locus_tag="OO609_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6632412.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4572..4955)
                     /locus_tag="OO609_000899"
     CDS             complement(4572..4955)
                     /locus_tag="OO609_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6632413.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4971..5279)
                     /locus_tag="OO609_000900"
     CDS             complement(4971..5279)
                     /locus_tag="OO609_000900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632414.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5280..5858)
                     /locus_tag="OO609_000901"
     CDS             complement(5280..5858)
                     /locus_tag="OO609_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6632415.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5881..6312)
                     /locus_tag="OO609_000902"
                     /pseudo
     CDS             complement(5881..6312)
                     /locus_tag="OO609_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6684..7703
                     /gene="epd"
                     /locus_tag="OO609_000903"
     CDS             6684..7703
                     /gene="epd"
                     /locus_tag="OO609_000903"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6632416.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7753..8916
                     /gene="pgk"
                     /locus_tag="OO609_000904"
     CDS             7753..8916
                     /gene="pgk"
                     /locus_tag="OO609_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6632417.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9131..10210
                     /gene="fbaA"
                     /locus_tag="OO609_000905"
     CDS             9131..10210
                     /gene="fbaA"
                     /locus_tag="OO609_000905"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6632418.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10401..11261
                     /gene="mscS"
                     /locus_tag="OO609_000906"
     CDS             10401..11261
                     /gene="mscS"
                     /locus_tag="OO609_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6632419.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11400..12035
                     /gene="argO"
                     /locus_tag="OO609_000907"
     CDS             11400..12035
                     /gene="argO"
                     /locus_tag="OO609_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6632420.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12128..12868
                     /locus_tag="OO609_000908"
     CDS             12128..12868
                     /locus_tag="OO609_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6632421.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13035..13985
                     /locus_tag="OO609_000909"
     CDS             13035..13985
                     /locus_tag="OO609_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6632422.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13927..15405)
                     /gene="scpC"
                     /locus_tag="OO609_000910"
                     /pseudo
     CDS             complement(13927..15405)
                     /gene="scpC"
                     /locus_tag="OO609_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15429..16214)
                     /gene="scpB"
                     /locus_tag="OO609_000911"
     CDS             complement(15429..16214)
                     /gene="scpB"
                     /locus_tag="OO609_000911"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6632423.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16225..17220)
                     /gene="meaB"
                     /locus_tag="OO609_000912"
     CDS             complement(16225..17220)
                     /gene="meaB"
                     /locus_tag="OO609_000912"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6632424.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17213..19357)
                     /gene="scpA"
                     /locus_tag="OO609_000913"
     CDS             complement(17213..19357)
                     /gene="scpA"
                     /locus_tag="OO609_000913"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6632425.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19561..20454)
                     /gene="argP"
                     /locus_tag="OO609_000914"
     CDS             complement(19561..20454)
                     /gene="argP"
                     /locus_tag="OO609_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6632426.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20837..21496
                     /gene="rpiA"
                     /locus_tag="OO609_000915"
     CDS             20837..21496
                     /gene="rpiA"
                     /locus_tag="OO609_000915"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6632427.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21752..22984
                     /gene="serA"
                     /locus_tag="OO609_000916"
     CDS             21752..22984
                     /gene="serA"
                     /locus_tag="OO609_000916"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6632428.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23213..23272
                     /gene="ibsC"
                     /locus_tag="OO609_000917"
     CDS             23213..23272
                     /gene="ibsC"
                     /locus_tag="OO609_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6632429.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23605..23664
                     /locus_tag="OO609_000918"
     CDS             23605..23664
                     /locus_tag="OO609_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6632430.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23765..24367)
                     /locus_tag="OO609_000919"
     CDS             complement(23765..24367)
                     /locus_tag="OO609_000919"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6632431.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24388..24571)
                     /gene="ssrS"
                     /locus_tag="OO609_000920"
     ncRNA           complement(24388..24571)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO609_000920"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24613..24942)
                     /gene="zapA"
                     /locus_tag="OO609_000921"
     CDS             complement(24613..24942)
                     /gene="zapA"
                     /locus_tag="OO609_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6632432.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25110..25688
                     /gene="ygfB"
                     /locus_tag="OO609_000922"
     CDS             25110..25688
                     /gene="ygfB"
                     /locus_tag="OO609_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6632433.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25714..27039
                     /gene="pepP"
                     /locus_tag="OO609_000923"
     CDS             25714..27039
                     /gene="pepP"
                     /locus_tag="OO609_000923"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6632434.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27036..28214
                     /gene="ubiH"
                     /locus_tag="OO609_000924"
                     /gene_synonym="visB"
     CDS             27036..28214
                     /gene="ubiH"
                     /locus_tag="OO609_000924"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6632435.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28237..29439
                     /gene="ubiI"
                     /locus_tag="OO609_000925"
                     /gene_synonym="visC"
     CDS             28237..29439
                     /gene="ubiI"
                     /locus_tag="OO609_000925"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6632436.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29513..29596)
                     /locus_tag="OO609_000926"
     CDS             complement(29513..29596)
                     /locus_tag="OO609_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632437.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29887..30981
                     /gene="gcvT"
                     /locus_tag="OO609_000927"
     CDS             29887..30981
                     /gene="gcvT"
                     /locus_tag="OO609_000927"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6632438.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31005..31394
                     /gene="gcvH"
                     /locus_tag="OO609_000928"
     CDS             31005..31394
                     /gene="gcvH"
                     /locus_tag="OO609_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6632439.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31513..34386
                     /gene="gcvP"
                     /locus_tag="OO609_000929"
     CDS             31513..34386
                     /gene="gcvP"
                     /locus_tag="OO609_000929"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6632440.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34653..35396
                     /locus_tag="OO609_000930"
     CDS             34653..35396
                     /locus_tag="OO609_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6632441.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35453..36886)
                     /gene="bglA"
                     /locus_tag="OO609_000931"
     CDS             complement(35453..36886)
                     /gene="bglA"
                     /locus_tag="OO609_000931"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6632442.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            36931..37241
                     /locus_tag="OO609_000932"
                     /pseudo
     CDS             36931..37241
                     /locus_tag="OO609_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37405..38064
                     /locus_tag="OO609_000933"
     CDS             37405..38064
                     /locus_tag="OO609_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6632443.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38260..39240)
                     /gene="ygfZ"
                     /locus_tag="OO609_000934"
     CDS             complement(38260..39240)
                     /gene="ygfZ"
                     /locus_tag="OO609_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6632444.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39483..39749
                     /gene="sdhE"
                     /locus_tag="OO609_000935"
     CDS             39483..39749
                     /gene="sdhE"
                     /locus_tag="OO609_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6632445.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39730..40137
                     /locus_tag="OO609_000936"
     CDS             39730..40137
                     /locus_tag="OO609_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6632446.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40177..40698)
                     /gene="fldB"
                     /locus_tag="OO609_000937"
     CDS             complement(40177..40698)
                     /gene="fldB"
                     /locus_tag="OO609_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6632447.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40810..41706
                     /gene="xerD"
                     /locus_tag="OO609_000938"
     CDS             40810..41706
                     /gene="xerD"
                     /locus_tag="OO609_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6632448.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41731..42441
                     /gene="dsbC"
                     /locus_tag="OO609_000939"
     CDS             41731..42441
                     /gene="dsbC"
                     /locus_tag="OO609_000939"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6632449.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42447..44180
                     /gene="recJ"
                     /locus_tag="OO609_000940"
     CDS             42447..44180
                     /gene="recJ"
                     /locus_tag="OO609_000940"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6632450.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44271..45369
                     /gene="prfB"
                     /locus_tag="OO609_000941"
     CDS             join(44271..44345,44347..45369)
                     /gene="prfB"
                     /locus_tag="OO609_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6632451.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45379..46896
                     /gene="lysS"
                     /locus_tag="OO609_000942"
     CDS             45379..46896
                     /gene="lysS"
                     /locus_tag="OO609_000942"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6632452.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46939..47487)
                     /gene="idi"
                     /locus_tag="OO609_000943"
     CDS             complement(46939..47487)
                     /gene="idi"
                     /locus_tag="OO609_000943"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6632453.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47542..47613
                     /locus_tag="OO609_000944"
     CDS             47542..47613
                     /locus_tag="OO609_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632454.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47610..47735)
                     /locus_tag="OO609_000945"
     CDS             complement(47610..47735)
                     /locus_tag="OO609_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632455.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47737..49185)
                     /gene="uacT"
                     /locus_tag="OO609_000946"
     CDS             complement(47737..49185)
                     /gene="uacT"
                     /locus_tag="OO609_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6632456.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11961 a        12395 c        13335 g        11841 t
ORIGIN      
        1 ttattgtcga agtgggtaaa aatctcactg gcaaatataa aaaaggccag cgttttgtat
       61 tgcaaccggc gatggggtta ccaagcggat attcagcggg ctacagctac gaatattttg
      121 gcggcaatgc cacttatatg attattcccg aaatcgccat taatttgggc tgcgtattac
      181 cgtatcacgg ctcttatttt gctgcggcgt cgctggcaga gcctatgtgc tgcattattg
      241 gtgcttatca tgccaattat cacaccacgc aatatgttta tgagcatcgc atgggcgtca
      301 aacctggcgg caatattgca ctgctggcgt gtgcgggtcc gatgggcatt ggcgctatcg
      361 attacgccat taacggtggc atacaaccgt cacgggtggt ggtggtcgat atcgacgaca
      421 aacgcctggc gcaggtgcag aagctgctgc cggtggatct ggcggccagt aaaggcattg
      481 agctggtgta tgtgaatacc aaagggatga gcgatcctat ccagatgctg cgggcgctga
      541 caggagatgt cgggttcgat gacatttttg tttatgcggc ggtgcctgct gtcgttgaga
      601 tggctgacga attactggcg gaagatggct gtctgaactt ctttgccggg ctgacggata
      661 aaaacttcaa agtgccgttt aatttctaca acgtccatta caacagcacg cacgtagtcg
      721 gtacatccgg cggttcaacg gacgacatga aagaggcgat tgcccttagc gccacaggac
      781 agttacagcc gtcgtttatg gtgacccata ttggtggcct ggatgcggtg ccagaaaccg
      841 tgctcaatct gccggatatc cctggcggta aaaaactcat ttataacggc gtgaccatgc
      901 cgctcactgc cattgccgat tttgccgaaa aaggcaaaac cgatccgctg tttaaagagt
      961 tggcgcggct ggttgaggaa acgcacggca tctggaatga acaggccgag aaatatctgc
     1021 tggcacaatt tggcgttgat atcggggagg ccgcgcaatg atgtccctgg cgtggccatt
     1081 atttcgcgtt acggaacagg cagcgttggc tgcctggccg caaaccggat gtggcgacaa
     1141 aaataaaatt gatggcctgg cggtcaccgc gatgcgccag gcattaaacg acgtcgcttt
     1201 tcgtggacga gtggttatcg gcgaaggaga aattgaccat gcgccgatgc tgtggattgg
     1261 cgaagaggtg ggcaaaggcg atgggccaga agtcgatatc gcggttgatc ccattgaagg
     1321 cacgcggatg gtggcgatgg ggcagagcaa tgcgctggcg gtcatggctt tcgctcctcg
     1381 agatagcctg ttacatgcgc ccgatatgta tatgaaaaag ctggttgtta atcgactggc
     1441 tgccggggcg attgatctgt ctttgcctct ggcggacaac ttgcgtaacg tggcgcgggc
     1501 gttaggtaaa ccgctggaca agctgcgcat ggttacgctg gataaaccgc gtctgagtgc
     1561 cgcgattgaa gaagcaacac agctgggcgt gaaggttttt gccctgccgg atggcgatgt
     1621 cgccgccagc gtgctgacct gctggcagga taatccttac gatgtgatgt acaccatcgg
     1681 cggtgcgcca gaaggcgtga tttctgcctg tgccgttaaa gcgttaggcg gggatatgca
     1741 ggccgagctg attgactttt gtcaggctaa aggggattac acggaaaatc ggcagattgc
     1801 agagcaggag cgcaagcgtt gtaaggcaat gggcgttgac gttaaccgtg tgtactcgct
     1861 cgatgaactg gtgaggggga acgatatcct ctttagcgct acgggcgtga cgggcggcga
     1921 gttggtgaac ggtatccaac agacggcgaa tggggtgcgg acgcagacat tactgatcgg
     1981 cggcgcggac caaacgtgta atataataga ctccctgcat tgatggtgat ctatcggaag
     2041 accaatggcg acgctgacag aagatgatgt gcttgagcaa ctggatgcac aggacaattt
     2101 attttcattt atgaaaactg cgcattctat tttgctccag gggatacgcc agtttctgcc
     2161 gtcgctgttt gtcgataacg atgaagagat cgtcgaatat gcagtgaagc cgttactcgc
     2221 ccaaagcggc ccgcttgacg atattgatgt tgcgctgcgt ttgatttatg cgctggggaa
     2281 aatggataaa tggctgtatg ccgatatcac gcacttttcc cagtactggc attacctgaa
     2341 cgaacaggat gaaacgcccg gatttgccga tgatataacc tgggatttta tcagcaatgt
     2401 caacagtata actcgcaatg cgacgctcta cgatgcgtta aaagcgatga agttcgccga
     2461 tttcgccgtc tggtcagagg cgcgttttag cggaatggtc aaaacggcgc tgacgctggc
     2521 agtaacgacc accttaaagg aattaacgcc gtgaaaattg aattaacggt gaatgggctg
     2581 aacgtgcaag cgcagtacca tgatgaagag atagaacgcg tgcataaact gttgctgcgt
     2641 atgctggcgg ccttacagac ggtaaatccg cagcggcgta cggtggtttt tctttgcgct
     2701 ccgccgggga cgggtaaatc taccctaacc accttctggg aatatctcgc gcaacaagac
     2761 ccggagctgc ccgctattca ggcgctcccg atggatggtt ttcaccatta caatagctgg
     2821 ctggacgcgc atcaattgcg ccccttcaaa ggcgcaccag agacatttga cgttgcgaaa
     2881 ctggcggaaa atctgcgcca ggtggtggaa ggggattgta cgtggccgca gtacgatcga
     2941 caaaagcatg atcctgttga agatgcgttg cacgttaccg caccactcgt catcgtcgaa
     3001 ggaaactggt tgttgctgga cgacgagaag tggtgccagc ttgcgcaatt ttgtgatttc
     3061 tcgatcttta tcaacgcgcc agctgctgcg ttgcgggagc gtctggtggg gcgcaagctg
     3121 gcgggagggg tatcgctggc agatgcagaa gccttttatg accgtaccga cgggccgaat
     3181 gttcgccggg ggctggaaga gagtctgcct gcaaatttga ccttaatgat gaccgctacg
     3241 ggtgaatatc gtctggtgga ttaacgttcc tgattacacc atcttccagc cgcaccacac
     3301 gagagaaatg acgccgggta aatgcggcgt catgactgat tgctaccacg ctcgtgcctc
     3361 gctggcggca cttctccagc cagctttcaa acacgctcaa ccaattttcg tcaaaatccc
     3421 gactcggctc gtccagcagt aatagtggcg gagagagtgc ttccaggcag gcgacggcga
     3481 ccatccgtcg ctgcgcatta tgcagatcca acgggtgcga gttggcagtg tctgctaact
     3541 gacaacattg cagggcagca tgggtacgtt gagtgatttc atccgccggg catttttgca
     3601 gtttcaaacc aaatgcgatc tcgtcagcga cggtgctgtg aaaaagttgg ttttccgctt
     3661 cctgaaacag tacgccaacc tttgccgcgc gctggcggtt tttcaggttt ttcatcgcct
     3721 gttgttgcag cataaccgta ccggctgtgg gcgtaagaag acctgccatt actcgtaaca
     3781 gcgtcgattt ccctgcgcca ttgtcaccgg tcagcgccaa ccattcacct tgtttgagtt
     3841 gcagagaaat gtcgcaaaga caatccgttg ccgcgccagg ccagcgataa gatatcttat
     3901 ttaacgttaa catagtggct ggagcgctcc gttttgcaat tgccacgctt gctggcagta
     3961 gttcaggaaa ggtgtgcgat gacgttcaaa aagaataatc agcgaaccac gttcaaacgc
     4021 ccagtgttgt aagcgctgca acagcatttc gctggcctgc ggtgtcaggc ggctaaaggc
     4081 ttcatcaaga attaagagct ttggctgcat cgcgatggcg caggcgatga tcacgcgctg
     4141 tgtctctcca ccggagagtg ttgccgggtg acgatggcgc aacggctggc actcggtcag
     4201 agtcagggcg gcatcaatcc gcgccataat ctctgcttca gcaagacaca gattttctgg
     4261 cccaaaggca acttcttctt ccacgctaaa ggtacagccg gagagctgca aatagggcga
     4321 ttgctggacg agctggatgg tggcggattg ttcattaagc gggagatgcc caatgggcgt
     4381 accgaggagc gtccccgtgc ctgttatctc tcttggcaga aagtccggat accagcccgc
     4441 catcagctgt gccagcgtac ttttgccact gccgttgtca ccaaagatgg cgaccatccc
     4501 cggtttgaca tagtgaaagt cataacagaa cggaggatgc gttgagtgtg tcgggcaata
     4561 acggaactgt tctaacgtaa ccatatccac gctccgaatt ctgtcagcat cagcaagatc
     4621 atggtgtaac gcgctacgcg ttgtagggtg ctatcggctg gtgcccacaa cgttgttcgt
     4681 cggttgtgga tccgaaatgc ccgcatatcc agcgccgcac cacggacagc cagatcgtta
     4741 agcgcattgt gtgttagcgg aataattaac gcgggcatag cacgtaaacg ctgataccag
     4801 ccctcatcca gcggcacgcc gcgggcacgc tgcgcttcat gaataatcgc taattgccgt
     4861 tttaactgct caacgaccaa taacggcccg gcaaacagat aagcgacgcc tggcggcagg
     4921 cgagaagcaa acagagcgcg aataaaacat tgcacgggga cgtactgcat tcacaactgt
     4981 gaggttgaaa caatcgccag aatcagcaac cagagcgtaa tagcatgcga ccaccgttct
     5041 ggcgagcgtg gcgtgccgct tagccattct ggtagccagc caccgtgtac cagccacagc
     5101 ccggctccca gcgagaacat cagccagacg acatattttg cccgccgccg cgtggccttt
     5161 agcgcaataa ggcagaaaaa ggtcgccgcg ctatagatcg gcaaaatggt ctgcgcgggc
     5221 aggataagcg tggtgcaggc cgctaatgcc cacagtgtta atgaggtaaa tggatgcatt
     5281 tagcgcacgg cagccattgc cggaaaatgg cgcgtggtgc gaatcggcag ctggcgcagt
     5341 aaaagccaga caatcaccgc tgtaagaatt ttatcgacca ggttcgcgcc tatcaccgta
     5401 atcgccaccg actccaccag gttttgcccc atcgagtgca tccaggcaac gaacaaatcg
     5461 gctccgctac cggtgacacc gccgaacagc gcagtacgca gcggtacagc gacgaccgtt
     5521 accgcaagcg taataatcac gccgctgaca acgacttttg gcagggtgcg aaaccaaccc
     5581 gctcgcgcca gccaaccgga gaccaggccg atgaccatcg cgacgggtgc aaatgcggcg
     5641 gcgatcgggt cggtcagcaa tccccagagt aaattggtca gtaagccggt cagcattccg
     5701 atcaccggac caagcagtac cgcgctaata agtgtgccga tggagtcgag aaatataggt
     5761 aatttgacca tgctggccag ttgcccgccg atcatattga tggcgataga tataacgatc
     5821 aacaccagtg cctgactgga aaagtggcta cgcgccataa gaaaacctct ttgaaagtag
     5881 ttatttaacg ggggaaatat gcaactcgtc gtaaccagaa agcgcgcaag gttggcggct
     5941 aaacgccagt tcctcaaggc taccgatcac cagttcaagc gtggtccgca cggtacagcc
     6001 gggcatcata tgaccgccaa gcatcgtgcc gtgtggatcg gaaacgcaga gatgaagatg
     6061 ctcaccactc tgttccagcg tgccgtttag tgagatcact tcgaatgtac cgttcaggag
     6121 cgtggtgcct tcttgcccgg catagcgcaa ggcgacgtca gtcaggctac cggtacagcc
     6181 tgcaatctag gcggcatgaa gttgatgttg ctgtgcaaaa gcgtgtagct gggaaaaaac
     6241 ttcctgcccg gggaggaggc gcagtgcgta aaagcgtgcg gaggaagaat atgagtaggg
     6301 caatgtcatc attggagtac tccgaaactc ggacgtattg cggataatat cgacgctgca
     6361 tcagtcatat tctgatataa gtcagggttt gtgacggcac aacgaaaaga ttttgctacg
     6421 cttattcgtg attctgcaaa gtggaataaa ttccacaggg gcggggctga aaacgtgacg
     6481 taagtcagat aattgctttc tttgactgga caatcattcc ttttattcca cgtttcgctt
     6541 atcctagctg aagcgtttca gtcgattaaa tgttcgacaa ttaaccaatc agtcgcagtt
     6601 tgcgacaggt aaggtttccc cggacgattt gctggattac tctgtctggc aaatttgctc
     6661 agggaaaacc ttgcaggaga tctatgaccg tacgcgtagc gataaatggc ttcggtcgca
     6721 tcgggcgtaa tgtggttcgt gctttgtatg aatccggacg ccgggcggaa attaccgtgg
     6781 tggcaatcaa cgaactggcg gatgctgcgg gcatggcgca tttgttgaaa tatgacacca
     6841 gccatggccg ttttgcatgg gaagtacgac aggaacgcga tcaacttttt gttggtgatg
     6901 acgccatccg cgtattgcat gaacgttcac tgcaatcgct cccctggcgt gaacttggcg
     6961 ttgatgtagt cctcgactgc accggcgtat atggctcccg cgagcatggc gaagcgcata
     7021 ttgccgccgg ggccaaaaaa gtgctctttt cacatcctgg cagtaacgat ctcgacgcga
     7081 ccgttgttta cggcgtcaat caggatcaac ttcgtgcgga acaccgcatc gtttctaacg
     7141 cttcctgtac cacgaattgc ataattcccg tcatcaaatt gttagatgat gcgtacggta
     7201 ttgagtccgg cactgtgacc acaattcact ccgccatgca cgatcaacag gttattgatg
     7261 cataccatcc tgacctgcgt cgcacccggg cagccagcca gtcgatcatt ccggtcgata
     7321 ctaaactggc cgctggtatc acacgatttt ttccgcaatt taacgatcgc tttgaagcga
     7381 ttgcggtacg tgtgccaacc ataaatgtga cggcaatcga tttaagcgtg acggtgaaga
     7441 aacctgtaaa agccaatgaa gtcaacctgt tgctgcaaaa agcagcacaa ggtgcatttc
     7501 atggtatagt tgactatacg gaattgccgt tggtctctgt agattttaac cacgatccgc
     7561 acagtgccat tgtcgatggc acccaaaccc gggtcagtgg cgcacacctg atcaaaacgt
     7621 tggtctggtg cgataacgaa tggggctttg ctaaccgaat gctcgacacg acgttagcta
     7681 tggctactgt tgctttcagg taagacgcaa gcagcgtctg caaaactttt agaatcaacg
     7741 agaggattca ccatgtctgt aattaagatg accgatctgg atcttgctgg gaaacgtgta
     7801 tttatccgtg cggatctgaa cgtaccagta aaagacggga aagtaaccag cgacgcgcgt
     7861 atccgtgctt ctctgccgac catcgaactg gccctgaaac aaggcgcaaa agtgatggta
     7921 acttcccacc tgggtcgtcc taccgaaggc gagtacaacg aagaattctc tctgctgccg
     7981 gttgttaact acctgaaaga caaactgtct aacccggttc gtctggttaa agattacctc
     8041 gacggcgttg acgttgctga aggtgaactg gttgttctgg aaaacgttcg cttcaacaaa
     8101 ggcgagaaga aagacgacga aaccctgtcc aaaaaatacg ctgcactgtg tgacgtgttc
     8161 gtaatggacg cattcggtac tgctcaccgc gcgcaggctt ctactcacgg tatcggtaaa
     8221 ttcgctgacg ttgcgtgcgc aggcccgctg ctggcagctg aactggacgc gctgggtaaa
     8281 gcactgaaag aacctgctcg cccgatggtg gctatcgttg gtggttctaa agtatctacc
     8341 aaactgaccg ttctggactc cctgtctaaa atcgctgacc agctgattgt tggtggtggt
     8401 atcgctaaca cctttatcgc ggcacaaggc cacgatgtgg gtaaatccct gtacgaagct
     8461 gacctggttg acgaagctaa acgtctgctg accacctgca acatcccggt tccgtctgat
     8521 gttcgcgtag caaccgagtt ctctgaaact gcaccggcta ccctgaaatc tgttaacgat
     8581 gtgaaagctg acgagcagat cctggatatc ggtgatgctt ccgctcagga actggctgaa
     8641 atcctgaaga atgcgaaaac cattctgtgg aacggtccgg ttggcgtgtt cgaattcccg
     8701 aacttccgca aaggtactga aatcgtggct aacgctatcg cagacagcga agcgttctcc
     8761 atcgctggcg gcggcgacac tctggcagca atcgacctgt tcggcattgc tgacaaaatc
     8821 tcctacatct ccactggcgg cggcgcattc ctcgaattcg tggaaggtaa agtactgcct
     8881 gcagtagcga tgctcgaaga gcgcgctaag aagtaaaaaa tcacagggca gggaaacctg
     8941 cacttgtttc agcgcgcttt tagagcacgc attttttcat actctaaata attcgagttg
     9001 caggaaggcg acaagagagt gaatccccag gagcttacat aagtaagtga ctggggtgag
     9061 cgaacgcaga cgcagcacat gcaacttgaa gtattatgag tataaggccc gacgatacag
     9121 gacaagagac atgtctaaga tttttgattt cgtaaaacct ggcgtaatca ctggtgatga
     9181 cgtacagaaa gttttccagg tagcaaaaga aaacaacttc gcactgccag cagtaaactg
     9241 cgtcggtact gactccatca acgccgtact ggaaactgct gctaaagtta aagcgccggt
     9301 tatcgttcag ttctccaacg gtggtgcttc ctttatcgct ggtaaaggcg tgaaatctga
     9361 cgttccgcag ggtgctgcta tcctgggcgc gatctctggt gcgcatcacg ttcaccagat
     9421 ggctgaacat tatggtgttc cggttatcct gcacactgac cactgcgcga agaaactgct
     9481 gccgtggatc gacggtctgc tggacgcggg tgaaaaacac ttcgcagcta ccggtaagcc
     9541 gctgttctct tctcacatga tcgacctgtc tgaagaatct ctgcaagaga acatcgaaat
     9601 ctgctctaaa tacctggagc gtatgtccaa aatcggcatg actctggaaa tcgaactggg
     9661 ttgcaccggt ggtgaagaag acggcgtgga caacagccac atggacgctt ctgcactgta
     9721 cacccagccg gaagacgttg attacgcata caccgagctg agcaaaatca gcccgcgttt
     9781 caccatcgct gcgtccttcg gtaacgtaca cggtgtttac aagccgggta acgtggttct
     9841 gactccgacc atcctgcgtg attctcagga atatgtttct aagaaacaca acctgccgca
     9901 caacagcctg aacttcgtat tccacggtgg ttccggttct actgctcagg aaatcaaaga
     9961 ctccgtaagc tacggcgtag tgaaaatgaa catcgatacc gatacccaat gggcaacctg
    10021 ggaaggcgtt ctgaactact acaaagcgaa cgaagcttat ctgcagggtc agctgggtaa
    10081 cccgaaaggc gaagatcagc cgaacaagaa atactacgat ccgcgcgtat ggctgcgtgc
    10141 cggtcagact tcgatgatcg ctcgtctgga aaaagcattc caggaactga acgcgatcga
    10201 cgttctgtaa gtcattcctt tctgcttatc tcaaggcccg ctctgcgggt ctttttttcg
    10261 ccaaaagcaa ggtgattcag atgagaaatc tgtgatctat ttggcaaaat tatgctttat
    10321 tgtttaccct tgtcagactg cccgtcataa ggcggcggag tgtatttctc cattttgagt
    10381 cagttgaaaa ggaatattga atggaagatt tgaatgttgt cgatagcata aacggcgcgg
    10441 gaagctggct ggtagctaac caggcgctgc tgctaagtta tgcagtaaac atcgtggcgg
    10501 cactcgcgat catcatcgtt ggtttgatta tcgcgcggat gatttccaac gcggtgaatc
    10561 gcctgatgat ctcccgtaaa atcgatgcca ctgttgctga ttttctttct gcattagtcc
    10621 gttacggtat tatcgccttt acgctaatcg ctgccctggg acgcgtgggt gtacaaacag
    10681 cgtcagtcat tgctgtactc ggtgccgcag gcttagctgt tggtctggct ttgcaggggt
    10741 cactttctaa cctggccgct ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggag
    10801 aatatgttga cctcggcggc gtagccggta ctgtgctgag tgtgcagatt ttctccacca
    10861 ccatgcgtac tgcagacggt aaaattatcg ttattccgaa cggtaaaatt attgccggaa
    10921 atattattaa cttctcccgc gagccagttc gccgtaacga atttattatt ggcgtggcgt
    10981 atgattccga tatcgatcag gttaagcaga tcctgaccaa tattatccag tctgaagatc
    11041 gcattttgaa agatcgcgaa atgactgtgc gcctgaacga acttggtgca tcgtcgatta
    11101 atttcgtggt ccgcgtctgg agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc
    11161 tggagcgtat taaacgtgaa tttgatgccg ccggtatcag cttcccgtac ccgcaaatgg
    11221 atgtgaactt taagcgggtg aaagaagaca aagctgcgta atcaacgctg aggccagata
    11281 atactctctg gcctctctct tattagttat tctgattgcc aattaatatt atcaatttcc
    11341 gctaataaca atcccgcgat atagtctctg catcagatac ttaattcgga atatccaacg
    11401 tgttttctta ttactttcaa ggtcttgctc ttggggcagc tatgattctg ccgctcggcc
    11461 cacaaaatgc ttttgtgatg aatcagggca ttcgtcgtca gtaccacatt atgattgcct
    11521 tactttgcgc tatcagcgat ttggtcctga tttgcgcggg gatttttggt ggtagcgcgt
    11581 tattgatgca gtcgccgtgg ttgctagcgc tggtcacctg gggcggcgta gtcttcttgc
    11641 tctggtatgg ttttggcgct tttaaaacag cgatgagtag taatattgag ttagctagtg
    11701 ccgaagtcct gaagcaaggc agatggaaaa ttatcgccac catgttggca gtgacctggc
    11761 tgaatccgca tgtttacctg gatacttttg ttgtactggg cagccttggc gggcagcttg
    11821 acgtcgaacc aaaacgttgg tttgcgctcg ggacaattag cgcctctttc ctgtggttct
    11881 ttggtctggc tattctcgca gcctggctgg caccgcgcct gcgcacggca aaatcacagc
    11941 gcattatcaa tctggtagtg ggatgtgtca tgtggtttat agccttgcag ctggcgagag
    12001 acggtattgc tcatgcacaa gccttgttca gttaggcgct atctgatgga aaaataaaac
    12061 agaggcgcta agcttgcctc cagaggtcct gaattttggg caagtcaatt aacacggaga
    12121 gactaacgtg aagttcaaag ttatcgccct ggcggcatta atgggtatta gcgggatggc
    12181 agcgcaggct aacgaattgc cggatggacc gcatattgtc acctccggta cggcaagtgt
    12241 ggatgcggtg ccagacattg ccactcttgc gattgaagtt aacgtggccg cgaaggatgc
    12301 cgctacagcc aagaaacagg cagatgagcg cgtcgcacaa tacatttcct tccttgaact
    12361 caatcagatc gcgaaaaaag atatcagctc agcgaattta cgcacccagc cagattatga
    12421 ctatcaggat ggtaaaagta tcctgaaagg ctaccgagct gtgagaacgg tggaagtcac
    12481 gctccgtcag ttagacaaac tgaattcctt gctggatggc gcgctgaagg cgggtcttaa
    12541 cgaaattcgt tctgtgtcgc tgggcgtggc gcagccggat gcctataaag acaaagcgcg
    12601 taaggcagcg attgataacg cgattcatca ggcgcaggaa ctggcgaacg gctttcatcg
    12661 taaactgggg ccggtatata gcgtgcgcta ccatgtttcc aactatcagc ccagcccaat
    12721 ggtgcggatg atgaaagccg atgccgcgcc ggtgtctgcc caggaaactt acgagcaggc
    12781 cgctattcag tttgatgatc aggtcgatgt ggtcttccag ttagaacctg tggatcaaca
    12841 acccgctaaa acacctgcag cacaataata cttgtagccc gacgtattcg tcgggctaat
    12901 gcctttaccc gatattgcct cctttttctg taattttctg taaaaccatg acgtaatcac
    12961 ctatctttgc tcgcggtttc atcaaggata ggctgactga aacggttaag cggacaagga
    13021 tgcggggagg ggggatggac atttttattt cgaaaaagat gcgcaatttc attttattag
    13081 cgcaaaccaa taatattgcc cgggcggcag aaaaaatcca tatgacggct tcgccatttg
    13141 gtaaaagcat tgccgcgctg gaagaacaaa ttggctatac gctatttacc cgcaaagata
    13201 ataatatcag cctcaataaa gccggtcagg aactgtatca aaaactgttc ccggtttatc
    13261 aacgactttc tgcgattgat aatgaaatcc ataactcagg gcgtcgttca cgagaaattg
    13321 tgattggcat agataatact tatccgacaa ttattttcga tcagttgatt agtctcggag
    13381 ataagtatga aggcgtgacc gcccagccag ttgagttcag cgaaaacggc gttatcgata
    13441 atctttttga ccgtcagtta gattttatta tctccccaca gcatgtatct gcacgagttc
    13501 aggaactgga aaacctgact atcagtgaat taccaccgct gcgattgggc tttctcgttt
    13561 ctcgccgtta tgaagagcgt caagagcaag agctgcttca ggagctgccc tggctacaaa
    13621 tgcgctttca aaaccgggct aacttcgaag caatgattga tgcgaatatg cgaccttgtg
    13681 ggatcaatcc caccattatt tatcgtccct acagttttat ggccaaaatc agcgccgtgg
    13741 agcgcggaca ttttctgacg gtgatcccgc attttgcctg gcgactggtg aatccggcaa
    13801 cgctgaaata ttttgatgcg ccgcacaggc cgatgtatat gcaggaatat ctttattcaa
    13861 tcagaaatca tcgttatacc gccacgatct tcagcatatt gctgaagatc gtgacgggac
    13921 aaaccattaa cccagcatcg agccggttgc aattaaatta cggtgtaagt cgaagacgtg
    13981 gctaagatcg tggtgaatat gtccgccggg cgcattttcc agatagcgat gcagataatc
    14041 ccgatacata ggatgtgcac aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag
    14101 accgcgcaga tcggcgatcc cttgttcggt gatgatcact ttgacgctgt gttcgctgtg
    14161 atcaacatgg ctgcacattg gcacgacggt tgagatcttg ccttctttag caatcgacgg
    14221 agccataaag atcgacagat acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt
    14281 catcagatcg accccggcta cgtgcgttga atttgcatgc ccgtaaatat caaactccag
    14341 gccgacgttc agagcgatga cgcccagacg acggatgatt tccgggttat tggaaatctc
    14401 ctgcggacgc aacacaatgc ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag
    14461 ggaatcggcc gagattgtca ggctggaggc gctggccccg ctgattttgc cggtttccag
    14521 taaatgcacc accgattcct gtagcacttc cgaatacatc atgaacggag gaatttccgg
    14581 gttttccccc agacgtgcca ttaccgcatt attgatattg cccacgccac tttgcagcgg
    14641 cagaaattcc ggcggaatac gcccatgcgc catttcctgc aataagaacg tgaccacgtt
    14701 atcggcaatc tgctggcaca tgggattttg cttatccagc atattaccgg cgtcgggcaa
    14761 gttggtttcc acgacggcga caatcttttt cggatcgatt tgcacatagc gggtaccgac
    14821 gcgatccatt gcatggaaga tcgacacgct attgcgccgc ggtggcgcgc caggaatcac
    14881 aatatctgcc agttctgcaa cgcgcggatc gtgatagtga ttaagttcaa tgatcacttt
    14941 cttcgctcgc agcagctagg tcggcgcatt accgatcccg ctggttaacc agactcgacc
    15001 atccggtgcc agtgccgatg cttcaatgac ggcaacatca atgtcgccga agaaaccgta
    15061 attgaccatt tgcgccactt cgctcaaatg caggtcaacg aaactcaccg cgccctgatt
    15121 gatcttttta cgtaaactgg acgatgtttg atatggcgca cgccaggaaa cagcatcggc
    15181 gtcagaaagt acatcgtcag cggcggcgct gattgacgcc cccgtcagca ggcgaatttg
    15241 atacggcttt ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg cgggtagggc
    15301 tttcggcgaa cccgctgggg taaagccgct aaatgccacc atgtcgttat gctggataat
    15361 ttctgccgct tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt
    15421 cgcagggatt aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcatccct
    15481 tcctggtaat cttcgctgtc ataaaccgcg cggcgcatcc cctgaatacg ttcaaattca
    15541 tcggagttca tggtgtgtgc ttcgcccagt acacgcagct cttctttgat aacggcaatg
    15601 gctaacggcg ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccagttct
    15661 tccacttcca caacatggtt gaggatgccg acagccagcg cgcgctgggc ggtgattggc
    15721 gaagcggtaa aaatcagctc tttgacaatg tggaagcccg cgtcgcgggt caggttgtga
    15781 atgccgacca ggttatacgg gacgccgagg tttacaggcg tcattgagaa ggttgaggta
    15841 ctggcggcga tgatcagatc ggaactcatg atcatttcaa atgcgccacc ccaaacacta
    15901 ccttccacca tcgaaatgat cggtttcggg aatttttgga tcatgcgggt gatttgacgc
    15961 aatggatcat cataggagag cggatcgcga ccgccagacg gcagttcgtg aatatcgtga
    16021 cctgcggaga agactttgga tccacttggc gcccgcaaaa tgatacagcg aatttccggc
    16081 cggttgagat cgcttaacgc ctgcataaga tcatcaataa agactttact taaggcatta
    16141 agttttcgtc catagttaaa ctcaatgacc gccactttgt tgatagtggc aacgttcaca
    16201 tactgataag acataaaaat tcctttaatc aaaatattgc gtctggataa attcactgag
    16261 ctgccgcagg ccggtgcgcg gtgagagcgt attgtttttg accgctaaaa gcgtctggcg
    16321 gtaatagcga tcgaaatctt cattcgcgaa caggtgattc agtacttctt cttcggtctg
    16381 cttacgcagc cattccaccg attgttgttg ccgcacttgt tgtaaacgac cactggcagt
    16441 tagcgcggtt ttgaagtcga tgatggcgtg ccagatctca tcgattccac gtttttccag
    16501 tgcgctacaa gtcagacccc gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag
    16561 ggcactctcg tacatatgcc gggcaatggc gacattggta tggttatcgc catcgtcttt
    16621 gttgataacg agcagatcag ccacttccat cagccctttt ttaatgccct gcagatcatc
    16681 gccgccaccg gcaatttgca acgagataaa acagtccacc atgcgggcga cttccgtttc
    16741 cgactgcgcg atgccaaccg tttcgacaat cactacgtca taacccgccg cttcgcataa
    16801 cagcattaat tcccgtgctc gctgactggc accgcccaga tgaccggagg agggtaccgg
    16861 gcgaataaac gccgcctcgg cacgcgccag gtcattcatg cgggttttat ccccgagaat
    16921 gctaccgcca gtgaccgggc tgctgggatc gaccgcaata acagcaacct ttaatccctc
    16981 acgaatcaac aacatgccaa aggcttcaag aaaggtactt ttccccgcgc cgggtgtgcc
    17041 ggtaacgccc agtcgcaggg cgttaccaca gaacggcata atagcgtcaa gcagctgtgt
    17101 acttagcgcc tgatgacgtg ggtgacggct ttccaccagc gtcatggctt gggcgagcgt
    17161 ggcacgctca ccctgacgta agcggcgaat actttctgcc agcgtggctt cattaatcat
    17221 gatgctggct tatcagattc agtacgtcgc gcacactgtc gagcataggt gtacctggac
    17281 cataaatcgc cgccacgccg cgctcttgca ggaaggcgta atcctgcggc ggaatgacgc
    17341 cacccgcgac cacgcagata tcttcgcgtc cccatttttt cagcgcttcg accagttccg
    17401 ggatcagcgt tttatgaccg gcagccagtg aggatgcgcc cactacgtga acgtcgtttt
    17461 ctacggctag gcgggcgatc tcttcaggtg tagagaacat cgggcttaaa tctacgtcga
    17521 aaccgagatc ggaataggcg ctggcgatca ctttcgcgcc gcgatcgtgt ccatcctggc
    17581 ccatcttagc gatcagaatg cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt
    17641 gcgcaacaat ggcatcgaac tcggaggccg atttctcaga ctgatgatag ctttgcgcaa
    17701 tcacgccggt aacacactgg cttggcacca gataacggtc gaaagcgact tccagcgcat
    17761 cggaaatttc acccagggtg gcgcgaacgc gagcggcatt aacagcggca gccagcaggt
    17821 tttcgttatg ctgtgcggcg tgagtcaggg cgttcaacgc ggcggttacg gcggcatcat
    17881 cacgggtggc gcgaatgcgt tccagcgaag caatttgctc gttacgcacc atcacgttgt
    17941 cgatctcaag tacatcggtt tcgtcttcgt gatccagttt gtacttgttg acaccaacga
    18001 tgacacgctt gccctggtcg atcagcgact gttcgcgcgc tgaggcctct tcgatcattc
    18061 gttttggcag acctgcttcg atcgctttcg ccatgccacc ggcttcgtcg atctgttgga
    18121 taatagctct ggcttgtttg acgatttgat cggtcagcga ctcaatgtaa taggatccgg
    18181 ccagtggatc gacggtgtgg cagagttctg attcttcctg gatgatgatc tgggtgttgc
    18241 gggcaatgcg tgctgagaaa tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat
    18301 gcagtgactg agtaccgccc agcgtcgcag ccagtgcttc aatggtggtg cggataacgt
    18361 tgttatacgg atcctgttca gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg
    18421 ccagtgattt cggatcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg
    18481 ccgcacgcaa catggcgacg ttcataaaca gatccatgcc gataccgaag aataacgaca
    18541 ggcgaggagc gaagtcatca attttcagtc cggcagagat tgctgctttg atgtactcaa
    18601 tcccatcggc gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcccccatgt
    18661 ggtaaccgct gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga
    18721 tgtcggcgat aatgcgcatt gacggttttg gtgggtaaat ataggtgttg cggcagaggt
    18781 actctttgag aatatcgttt tgaatggtgc cggtcagttt atcaggtgta acaccttgct
    18841 cttctgcggc gacgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg
    18901 aaaccgacat tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt
    18961 cgatagcgac gcccgctttg ccgacgtcac ccgccacgcg tgggttatcg gagtcgtagc
    19021 cacggtgggt ggcaaggtca aacgcaacgg aaagaccttt ttgcccggcg gccaggttac
    19081 ggcgataaaa agcgttggac tcttttgctg ttgaaaaacc agcatactga cggatggtcc
    19141 acggttgggc ggtatacata gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa
    19201 gggtacctgt cacctccaga ttatcgagat cggcttcggt atacagcggc ttgatggcga
    19261 tcccttccgc ggtttgctga accagcgagt cgacagtttt ctcccgacgg ctcaattcct
    19321 tgttggcaag ctgttgccac tcctgctcgt tagacattat ttgctccgta aaacgcaatc
    19381 aaaatgaggg tatttgcgct tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa
    19441 aatgcattca gacgtcgttt tttgccgcct gagatttgtc tattttgtga tgtgattcaa
    19501 ggaatacaaa aagaagtgag gacaacgcct gatatgtgct atcaggcatt atttgatgga
    19561 ttaatcctga cgaaggactt tgtgaccata atcgagtaac gcatcagtga ctttacgcat
    19621 catgcggctt tcaggagcaa agcggtgcca gtagagcatc cgtcgttgaa atagcccagg
    19681 cgttaagtca atcagttcac cgctggccag ctctttctcg atttgcaggt gcgggatcat
    19741 acagcaggtg gtgccctggc gagcaagttg tacgaacgct tctgaagaat taacgatatg
    19801 gcagggtacg ctgccaggag gaagatcgaa gttttgctgc aaaaaggcct ggtgcatatc
    19861 gtcaagatgg tcaaacgcga ccactggcgc tttcaataat gccgaacgcg ttacgccgtt
    19921 aggaaaatat ttttcggcaa acggttttga gctgacgaac agatagtcga gcgcaccgag
    19981 tttatcgaca agacaactcg gcagcgcctg atgttgaata ctcaccgcgc cgaccacttc
    20041 gccgcggcgc agacgttcct gagtacgggt ttcatcttct acctgcaagt tgaggcggat
    20101 aggcgaatca gccaacacag gagccagtgc cggaagcaac cacgtcgcca gactgtcggc
    20161 gttgaccgcc agtgaaagca gcagcggagt cgaaccggtt tgttcatcgc ccagccactc
    20221 ttcttccagc aactccacct ggcgcagcag tgccagcagt ttttgccctt gttccgtcgg
    20281 gcgcggcggt acggtacgca ccaacagcgg ctgcccgaac atattttcca gttgcttaat
    20341 gcgctgtgag acggctgatt gtgtaatgca cagcttttgt gccgcgcgct caaatcctcg
    20401 ttcacgtatc accgcatcca gtgcctgtaa tgttctgtag tccgggcgtt tcattgctgt
    20461 tgtctgctcc tggtaaatgc tttttcctgc actatgacac aattttgtgt caggttgcat
    20521 ataccgaagc cgtcggcggg taacatttgt atcaatggtt aaacgcgcag cttaaaagat
    20581 cagggatttg cgctattgca tttcgcttag cgacttcgcg ccctggttat gttgaatggc
    20641 gtggcgttat tgcctctgtt cgcaccgtgg agcggcatac ccagaccgtt gtatgcttta
    20701 tttgcggggc gaaaggggat gcccgccatt gcgcgatatt ttatggatga gttaaccacc
    20761 cggcttgcca acggggtctg aatcgctttt tttgtatata atgcgtgtga aatttcatac
    20821 cacaggcgaa acgatcatga cgcaggatga attgaaaaaa gcagtaggat gggcggcact
    20881 tcagtatgtt cagcccggta ccattgttgg tgtaggtaca ggttccaccg ccgcacactt
    20941 tattgacgcg ctcggcacga tgaaaggcca gattgaaggg gccgtttcca gttcagatgc
    21001 ttccactgaa aaactgaaaa gcctcggcat tcacgttttt gatctcaacg aagtcgacag
    21061 ccttggcatc tacgttgatg gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg
    21121 cggcggcgcg gcgctgaccc gtgaaaaaat cattgcttcg gttgcggaaa aatttatctg
    21181 tattgccgac gcctccaagc aggttgatat tctgggtaaa ttcccgctgc cagtagaagt
    21241 tatcccgatg gcacgtagtg cagtggcgcg ccagctggtg aaactgggcg gtcgtccgga
    21301 ataccgtcag ggcgtggtga ccgataatgg caacgtgatc ctcgacgtcc acggcatgga
    21361 aatccttgac ccgatagcga tggaaaacgc cataaatgcg attcctggcg tggtgactgt
    21421 tggcttgttt gctaaccgtg gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa
    21481 aaccattgtg aaatgatctg acgggggaac ctcccccgtt aaaaaaattc tcttcattaa
    21541 atttggtgac atgtgtcacg cttttaccaa gcaattgtcg attgctctaa ataaatcctc
    21601 taaaccagca tattcatcca agaattacct ttgcgtgata tttcctcaac atcgcgacgc
    21661 aaacgttcat attgccgcaa tattattttt tgatatgttg aaaggcggat gcaaatccgc
    21721 acacaacatt tcaaaagaca ggattgggta aatggcaaag gtatcgctgg agaaagacaa
    21781 gattaagttt ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc
    21841 tggttacacc aacatcgaat ttcacaaagg cgcgctggat gatgaacaat taaaagaatc
    21901 catccgcgat gcccacttca tcggcctgcg atcccgtacc catctgactg aagacgtgat
    21961 caacgccgca gaaaaactgg tcgctattgg ctgtttctgt atcggaacaa accaggttga
    22021 tctggatgcg gcggcaaagc gcgggatccc ggtatttaac gcaccgttct caaatacgcg
    22081 ctctgttgcg gagctggtga ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc
    22141 caatgctaaa gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg
    22201 cggcaaaaag ctgggtatca tcggctacgg tcatattggt acgcaattgg gcattctggc
    22261 tgaatcgctg ggaatgtatg tttactttta tgatattgaa aacaaactgc cgctgggcaa
    22321 cgccactcag gtacagcatc tttctgacct gctgaatatg agcgatgtgg tgagtctgca
    22381 tgtaccagag aatccgtcca ccaaaaatat gatgggcgcg aaagagattt cgctaatgaa
    22441 gcccggctcg ctgctgatta atgcctcgcg cggtactgtg gtggatattc cggcgctgtg
    22501 tgatgcgctg gcgagcaaac atctggcggg ggcggcaatc gacgtattcc cgacggaacc
    22561 ggcgaccaat agcgatccat ttacctctcc actgtgtgaa ttcgacaacg tccttctgac
    22621 gccacacatt ggcggttcga ctcaggaagc gcaggagaat atcggcttgg aagttgcggg
    22681 taaattgatc aagtattctg acaatggctc aacgctctct gcggtgaact tcccggaagt
    22741 ctcgctgcca ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc gtccgggcgt
    22801 gctaactgcg ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg cgcaatatct
    22861 acaaacttcc gcccagatgg gttatgtagt tattgatatt gaagctgacg aagacgttgc
    22921 cgaaaaagcg ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc gtctgctgta
    22981 ctaattcccc ttctctgaaa attaacgggc aggtcactga cttgcccgtt tttttatccc
    23041 ttctccacac cgttgccgcg ttgcgcattc tactttgcga gtctgctcca gaaaataaat
    23101 caaagtcaga tcaatgcgtt gtgtttattt aatggggatt tacgatggcg ggacagcatg
    23161 gggctgtaac ggtaaagccc tcaccgaagc gagggcttaa aggagaaggg ttatgatgcg
    23221 acttgtcatc atactgattg tactgttact gataagtttc ggagcttatt aacagtcagt
    23281 ctcaagggag gagtcatcct cccttaccct tactcactaa attaggtcaa agaatcaacg
    23341 atgtcaatca gggcgatgcg gggtgcatcg ccctgaaccg aactttttga tagccacttc
    23401 atcgcccacg tttactgccg taatagatcc tttttcccaa aaattatctc tgttttgcat
    23461 ggggtttttc gagcacgctc gaagaaaaga tttaaaggta gatcaatgcg ttgtggttat
    23521 ttaaagagag cttacgatag ctgggcagca tggggctgta acggtaaagc cctcaccgaa
    23581 gcgagggctt gaaggagaag ggttatgatg cgacttgcca tcatactgat tgtactgtta
    23641 ctcataagtt tcccagctta ttaacagtca gtcacagggg aggagcaatc ctcccttacc
    23701 cttactcact aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg caggtgtatc
    23761 gcccttacca ctcccagact ttcgacggtg taaccaccgc aggaagaggg atatcccact
    23821 cttcaacggg gagtttttcc accaactgac aatcatgcgc ataacccacc ggttgcgttt
    23881 tatagtgctg ccagttttgt aaggtccgat cataaaaacc accgcccatt cccagacgct
    23941 gaccgtactc atcaaaggcg accagcggtg tgatcagcac gtctaatcgg gaaaggggta
    24001 gcacgtcacg cacatccagt tttggctcat ggatcttcaa cctgttcatc accagttcgc
    24061 tttgcggatg gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa
    24121 gatatacgcg cttaccggcg cgccagagtt gttctattag cggctgggtg tcgagttcac
    24181 catcaaaaga gaggaataca gcgaccgtat gcgccatcac caccggggga taagtcatca
    24241 tccgggtagc ggcttgttga cccatttcca gctgttgttc cggcgttaac gcacgacgac
    24301 gttgccgaat cattttgcgg atttcttgtc gggataatgt cagtgggagc tctggtagtt
    24361 gcgtcatggc tggtgccaga taagaaggga atctccgaga tgccgccgca ggctgtaacc
    24421 cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacgaacc
    24481 gagcatgctc accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg
    24541 actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta
    24601 aaccgaaaag tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag
    24661 taacgcttgt tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta
    24721 gtcacgagtc tttgcttttt cttgcgctaa ctcatagctg atattcaatg cggcaatgaa
    24781 gaccaactgt tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt
    24841 cagatcgtcc gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag
    24901 tgaacggcca aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt
    24961 gactgcgctg ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc
    25021 tgatgagaag agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg
    25081 aatattcctc cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca
    25141 acgaaatgaa ccagtatctg aaccaacaag ggacgggtct gacccctgcc gagatgcatg
    25201 gtttaatcag tgggatgata tgtggcggta acgatgacag ctcatggctg ccgctacttc
    25261 acgacctgac gaacgaaggc atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa
    25321 tgcactctgc caccagcgat gccctgcagg atgacggctt cctttttcag ctttatctgc
    25381 ctgatggcga tgatgtcagc gttttcgatc gggctgatgc actggcaggt tgggtcaatc
    25441 acttcctgct tggtcttggc gttacgcaac cgaagctgga caaagtgacc ggcgaaaccg
    25501 gtgaagccat cgacgatctg cgtaacatcg cgcagctggg ctacgacgaa gacgaagatc
    25561 aggaagagct tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt
    25621 tatgccacga cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc
    25681 tacactaaaa acaaaaaacg taaggagagt gttatgagtg agatatcccg gcaagagttt
    25741 cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc gctgattttt
    25801 gctgcaccag aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac
    25861 ttctggtact tcaccggctt taacgaaccg gaagcggtgc tggtgctgat taaaagcgat
    25921 gacactcata accacagcgt tctgtttaac cgcgttcgcg acctgacggc ggagatctgg
    25981 tttggccgtc gcttaggcca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg
    26041 gcattcagcg aaatcaatca gcaactttat caactactta acggcctgga tgtggtttac
    26101 catgcccagg gcgaatatgc atatgctgat gaaatcgtga acagtgcgct ggaaaaactg
    26161 cgtaaaggtt cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt
    26221 gttcatgaaa tgcgcctgtt caaatcgcca gaagagattg ccgtactccg ccgcgcggga
    26281 gaaatcaccg ccatggcaca tacacgggcg atggaaaaat gccgtccggg aatgttcgag
    26341 taccatctgg aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg ctatccttcc
    26401 tataacacca ttgtaggcag cggtgaaaac ggctgcattc tgcactacac cgaaaacgag
    26461 agtgaactgc gcgacggtga cctggtgttg attgacgcgg gttgtgaata caaaggttac
    26521 gcgggcgata ttacccgcac cttcccggtc aacggcaaat tcacccaggc ccagcgtgaa
    26581 atctacgaca ttgtgctgga gtctctcgaa accagcctgc gcctgtatcg tccgggaact
    26641 tccattctgg aagtcactgg tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc
    26701 ggcatcctga aaggtaatgt tgatgaactg atcgctcaga acgcccatcg tcctttcttt
    26761 atgcatggcc ttagccactg gttaggactg gatgtccatg acgttggcgt ttatggtcag
    26821 gatcgctcgc gcattctgga accggggatg gtactgaccg tagagcctgg tctgtatatc
    26881 gcgccggatg cggatgtgcc agaacaatat cgcggtatcg gcattcgtat tgaagacgac
    26941 attgtgatta ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa
    27001 gaaatcgaag cgttgatggc tgctgcgaga aagcaatgag cgtaatcatc gtcggtggcg
    27061 gcatggcggg cgcgacgctg gcgctggcta tttcccggtt aagtcacggg gcgctgccgg
    27121 tacatttgat tgaagcgact gcgccagagt cacatgctca tccgggcttt gatggacgag
    27181 cgatagcgct ggcggcgggt acctgtcagc aactggcgcg catcggcgtc tggcaatctc
    27241 tggctgattg cgcaaccgcc atcactaccg tgcatgtcag cgatcgtggt catgctggat
    27301 tcgtcaccct cgccgcagaa gattaccaac tggcggcgct ggggcaggtt gtcgaattgc
    27361 ataatgtcgg gcaacggctg tttgcattgc tgcgaaaagc acctggcgta atgctgcatt
    27421 gccccgatcg cgtagctaac gttgcccgta ctcagagtca tgttgaagtg acgctggaga
    27481 gcggcgagat gctgacgggc cgcgtgctga tagccgctga tggcacccat tcagcgttag
    27541 ccaccgcgtg cggcgttgat tggcagcagg agccttacga acaactggca gtgattgcca
    27601 acgttgctac ttctgttgcg cacgaaggcc gcgcttttga acgcttcacg caacatggcc
    27661 cgctggcgat gttgccgatg tctgacggac gctgttcgct ggtctggtgt catccactgg
    27721 aacggcgcga agaggtgctg tcgtggagcg acgagaagtt ttgccgtgaa ctccagtctg
    27781 cctttggctg gcgactgggg caaattaccc acgccggtaa acgcagtgct tatccgctgg
    27841 cattaacccg cgccgccaga ccgattaccc atcgcactgt gctggtgggc aatgcggcgc
    27901 aaactctgca cccgattgcc gggcaagggt ttaacctcgg tatgcgagat gtcatgagcc
    27961 ttgcggaaac cctgactcag gcgcatgagc gcggagaaga catcggtgat tacggcatat
    28021 tgtgccgcta tcagcagcgt cgacagagcg atcgcgaagc aaccatcggc gtcacggaca
    28081 gcctggtaca tctttttgcc aaccgttggg caccgctggt tgtcgggcgc aacatcggac
    28141 tgatgacgat ggaattattc accccggcac gcgatgtgct ggcgcagcgc accctcggtt
    28201 gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga tgtagccatt
    28261 gttggcggcg gcatggtggg gctggcggtt gcctgcggct tacaggggag cggcttacgc
    28321 gttgccgtac tggagcagcg cgtaccggaa cctctggcgg tagatgcact accacaactg
    28381 cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca cccgtcttgg cgtctggcag
    28441 gacattctcg ctcgtagggc cagttgttat cacggcatgg aagtgtggga caaagacagt
    28501 tttggtcaca tctcgtttga cgatcaaagc atgggctata gccatcttgg gcacatcgtt
    28561 gaaaactcag tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc agatattact
    28621 ctgttggccc ccacagaatt acagcaggtc gcctggggag aaaatgaaac cttcctgacg
    28681 ctgaaagatg gcagcatgtt aacggcgcgt ctggtgattg gcgcggacgg tgctaattcc
    28741 tggttgcgca acaaagcaga tattccgctg actttctggg attatcagca tcacgcgctg
    28801 gtagcgacca ttcgcacgga agaaccgcat gatgcggtgg cgcggcaggt tttccatggc
    28861 gaaggcattc tggccttttt accgcttagc gatccgcatc tttgctcgat tgtctggtca
    28921 ctgtcgccag aggaagcgca gcggatgcag caggcaagtg aagacgaatt taatcgcgcg
    28981 ttaaatatcg cttttgataa tcgccttggc ttatgcaagg ttgagagcgc gcgtcaggtg
    29041 ttcccgctga cggggcgtta tgcgcgccag tttgccgcac accgtctggc gctggtgggc
    29101 gacgccgcgc ataccattca cccactggcg gggcagggcg ttaatctcgg atttatggac
    29161 gccgcagagc tgattgctga actgaaacgg ttgcatcgtc agggtaaaga catcgggcag
    29221 tacatttacc tgcgccgcta tgaacgtagc cgcaagcaca gcgcggcgct gatgctggct
    29281 ggtatgcagg gttttcgcga tctcttttcc ggcgctaatc cggcgaaaaa actgctgcgt
    29341 gatattggtt tgaaactggc cgacacgctt cctggcgtta agccgcaact tatccgccag
    29401 gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa aatttctcct ctgttgttta
    29461 tttgataccc atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga
    29521 aataaactaa tttcacctcc gttttcgcat tatattttct aataccatga ttttttgatt
    29581 tagtgttttt tgacattttt ttagctctta atattgtctt attcaaattg actttctcat
    29641 cacatcatct ttgtatagaa attggtgtat tttttggttt tttattctgt cgcgattttt
    29701 gcatttttta accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt
    29761 tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg
    29821 ctgggttttg tggtgataat ttcaccatga aaaagttgtc agccccgctt attcaatgag
    29881 gacaagatgg cacaacagac tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg
    29941 gtggatttcc acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat
    30001 gcggtacgta ccgatgccgg aatgtttgat gtgtcgcata tgaccatcgt cgatctccgc
    30061 ggcagccgca cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc
    30121 aaaagcggca aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgat
    30181 ctcatcgtct actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc
    30241 gaaaaagacc tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt
    30301 cgtgatgacc tttccatgat tgccgtgcaa gggccgaatg ctcaggcaaa agctgccaca
    30361 ctgtttaatg atgctcagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag
    30421 gtgggcgatc tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg
    30481 ctgcccaatg aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg cgttaagccc
    30541 tgcggtctgg gggcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag
    30601 gagatggacg aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa
    30661 ccgacagatc gtgactttat cggtcgtgaa gccctggaag tgcagcgtga gcatggtaca
    30721 gaaaaactgg ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta
    30781 cgctttaccg atgcgcaggg caaccagcat gaaggcatta tcaccagcgg tactttctcc
    30841 ccgacgctgg gttacagcat tgcgctggcg cgcgtgccgg aaggtattgg cgaaacggcg
    30901 attgtgcaaa ttcgcaaccg tgaaatgccg gttaaagtga caaaacctgt ttttgtgcgt
    30961 aacggcaaag ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag
    31021 cagaactgaa atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca
    31081 ccgttggtat taccgaacat gctcaggagc tgttaggcga tatggtgttt gttgacctgc
    31141 cggaagtggg cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcggtaaaag
    31201 cggcgtcaga catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga
    31261 gcgattcccc ggaactggtg aacagcgaac cgtatgcagg cggctggatc tttaaaatca
    31321 aagccagcga tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt
    31381 tagaagatga gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggaaaatt
    31441 tatgcctcac cctcactctc ttcgtaagga gagaggttca caattcactg cacgtttcag
    31501 gaaccatcgc tcatgacaca gacgttaagc cagcttgaaa acagcggcgc ttttattgaa
    31561 cgccatatcg gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt tggtgcacaa
    31621 tcgttaaacg cgctgaccgg ccagattgtg ccgaaagata ttcagcttgc gacaccaccg
    31681 caggttggcg caccggcgac cgaatacgcc gcactggcag aactcaaggc tattgccagt
    31741 cgcaataaac gcttcacgtc ttacatcggc atgggttaca ccgccgtgca gctaccgccg
    31801 gttatcctgc gtaacatgct ggaaaatccg ggctggtata ccgcgtacac tccgtatcag
    31861 cctgaagtct cccagggccg ccttgaagca ctgctcaact tccagcaggt aacgctggat
    31921 ttgactggac tggatatggc ctctgcttct cttctggacg aggccaccgc tgccgccgaa
    31981 gcaatggcga tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt cttcgtggct
    32041 tccaatgtgc atccgcaaac gctggatgtg gtccgtactc gtgctgaaac ctttggtttt
    32101 gaagtgattg tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt cggcgtgctg
    32161 ttacagcagg taggcactac cggtgaaatt cacgactaca ctgcgcttat tagcgaactg
    32221 aaatcacgca aaattgtggt cagcgttgcc gccgatatta tggcgctggt gctgttaact
    32281 gcgccgggta aacagggcgc ggatattgtt tttggttcgg cgcaacgctt cggcgtgccg
    32341 atgggctacg gtggcccaca cgcggcattc tttgcggcga aagatgaata caaacgctca
    32401 atgccgggcc gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc gctgcgcatg
    32461 gcgatgcaga ctcgcgagca acatattcgt cgtgagaaag cgaactccaa catttgtact
    32521 tcccaggtac tgctggcaaa catcgccagc ctgtatgccg tttatcacgg cccggttggc
    32581 ctgaaacgta tcgctaaccg cattcaccgt ctgaccgata ttctggcggc gggcctgcaa
    32641 caaaaaggtc tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt ggaagtggcc
    32701 gacaaagcgg gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg tagcgatatc
    32761 ctgaacgccg tggggatcac ccttgatgaa accaccacgc gtgaaaacgt aatgcagctt
    32821 ttcagtgtgc tgctgggcga taaccacggg ctggagatcg acacgctgga caaagacgtg
    32881 gctcacgaca gccgctctat ccagcctgcg atgctgcgcg acgacgaaat cctcacccat
    32941 ccggtgttta atcgctatca cagcgaaacc gaaatgatgc gctatatgca ctcgctggag
    33001 cgtaaagatc tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac catgaaactg
    33061 aacgccgccg ccgagatgat cccaatcact tggacggaat ttgccgaact gcacccgttt
    33121 tgcccgccgg aacaggctga aggttatcag cagatgattg cgcagctggc tgactggctg
    33181 gtgaaactga ccggttacga cgccgtttgt atgcagccga actccggcgc acagggcgaa
    33241 tacgcgggcc tgctggcgat tcgtcattat catgaaagcc gcaacgaagg gcatcgcgat
    33301 atctgcctga tcccggcttc tgcgcacgga actaaccccg cttctgcaca tatggcagga
    33361 atgcaggtgg tggttgtggc gtgtgataaa aacggcaaca tcgatctgac tgatctgcgc
    33421 gcgaaagcgg aacaggcggg cgataacctc tcctgtatca tggtgactta tccttctacc
    33481 cacggcgtgt atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca gttcggcggt
    33541 caggtttacc ttgatggcgc gaacatgaac gcccaggttg gcatcacctc gccgggcttt
    33601 attggcgcgg acgtttcgca cctcaacctg cataaaactt tctgcattcc gcacggcggt
    33661 ggtggtccgg gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt tgtaccgggt
    33721 catagcgtgg tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc tgcggcaccg
    33781 ttcggtagcg cctctatcct gccaatcagc tggatgtaca tccgcatgat gggcgcagaa
    33841 gggctgaaaa aagcaagcca ggtggcaatc ctcaacgcca actatattgc cagccgcctg
    33901 caggatgcct tcccggtgct gtataccggt cgcgacggtc gcgtggcgca cgaatgtatt
    33961 ctcgatattc gcccgctgaa agaagaaacc ggcatcagcg agctggacat tgccaagcgc
    34021 ctgatcgact acggtttcca cgcgccgacg atgtcgttcc cggtggcggg tacgctgatg
    34081 gttgaaccga ctgaatctga aagcaaagtg gaactggatc gctttatcga cgcgatgctg
    34141 gctatccgcg cagaaattga ccaggtgaaa gccggtgtct ggccgctgga agataacccg
    34201 ctggtgaacg cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca tccgtacagc
    34261 cgtgaagttg cggtattccc ggcaggcgtg gcagacaaat actggccgac ggtgaaacgt
    34321 ctggatgatg tttacggcga ccgtaacctg ttctgctcct gcgtaccgat tagcgaatac
    34381 cagtaattca ctgattcgag tattttctaa aggcgcttcg gcgccttttt agtcagatga
    34441 caaagtacaa aagtgctcag acagtcccct cgcccctttg gggagagggt tagggtgagg
    34501 ggaacaggcc ggcactggcg cgaatattta ccctcacccc gaccctctcc ctgaaagggc
    34561 gagggggaaa agcgtgccaa cattgaagat tgagccagtt tgttagcaat ctcaaagata
    34621 cgtcaacgaa ttgatttttc tcggaaaaac aaatggctat agcacttgtg accggtggca
    34681 gtcgcggcat cgggcgggca actgcattac tgttggcgca agaagggtat acggtggcgg
    34741 ttaattatca gcaaaacctc cacgcggcgc aggaagtgat gaacttaata atgcaagccg
    34801 gtggcaaagc attcgtgctc caggcggata tcagcgacga aaaccaggtc gttgcgatgt
    34861 ttacagcaat cgatcagcac gatgaaccgc tagcagcgct ggtcaataac gccgggatct
    34921 tgtttaccca gtgcaccgtt gaaaacctta ccgcagagcg aatcaaccga gtactttcca
    34981 ccaacgtgac gggatatttt ctctgctgcc gcgaggcggt aaaacgcatg gcgcttaaaa
    35041 atggtggcag tggcggcgct atcgtcaatg tctcttcggt ggcctcacgg ttgggttcgc
    35101 caggggaata tgttgattac gcggcatcga aaggggcgat tgattcgtta accaccggac
    35161 tatcgctgga agtcgccgcg caggggatcc gcgttaactg cgtgcggcca gggtttattt
    35221 ataccgaaat gcacgccagc ggcggcgagc ctggacgcgt cgatcgcgtt aagtcgaaca
    35281 tccccatgca gcgtggtgga caggcagaag aggtcgcgca ggccattgtc tggctactaa
    35341 gtgataaagc ctcttacgtc acgggaagtt ttatcgattt ggcgggcggg aaataaaaca
    35401 gggaagttgt ctgaccggat gcaacaagta ttgcatccgg tacttcatcg acttaaagct
    35461 tctcgccgtt gctggcaatc acctctttgt accagttaaa gctcttctta cgtgaacgcg
    35521 acatatcgcc agtaccgtcg tcatgtttat tcacatagat aaagccgtag cgtttgctgt
    35581 actgcccggt agtgaatgac acgcagtcga tgcagcccca cggtgtgtag cccatcagat
    35641 ccacaccatc gtaagtcact gcttttttca tctcttcaat atgggcgcgc aggtagtcaa
    35701 tgcggtagtc gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt
    35761 tttcgacaat aaacagcggc ctctgataac gctcatacag ttcgcaaagg gcatagcgca
    35821 gacctactgg atcaatctgc cagccccagt cagacgcttt aacatacggg ttcggtacgc
    35881 tgccttcaaa accagagatc gcatcgccgg tgccgccttc ggccttcact gcgttggtca
    35941 tgtaatagct gaaaccaaga taatcgcagg tgccttcgcg cagtacatcg agatcgccgt
    36001 cttccatttt gatattaaat ccgcgacgtt cccactcgtt caatacatag gacgggtaat
    36061 agccgcatag ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca
    36121 tcacatcgtc cgggttacat gagtagggat agagcggcac catcgccagc atacagccga
    36181 ctttcatctc cgggttaatg cgacgcgcgg ctttcactgc cagggcgctg gcgacaaact
    36241 ggtgatgcag cacctggtac atcgtctctt ccgggttgtc atgctcggta tacaccacgc
    36301 cggagcagca gtaaccaaac agcggcgcac gccagttacg ctggttgttt atttcgttga
    36361 aggtcatcca gtatttgact ttatgcttgt agcgttcgaa taccacttcc gcgaaacgga
    36421 caaagaaatc aacgaattta cggttggtcc agctaccgta ttgctgcacc agatgcagcg
    36481 gcatttcaaa gtgggagagg gttatcaccg gttcgatgtt gtatttcagc agttcatcga
    36541 acagagagtc gtaaaacttc agcccttctt cgtttggctg agcttcatca ccttttggaa
    36601 aaatacgcgt ccaggcgatg gaagtccgaa aacatttgaa gcccatttcg gcaaatagct
    36661 tgatgtcttc cttgtagtga ccataaaaat caacggcttc atggtttgga tagtattttc
    36721 ctggcaagac ttctttggtg atttcgcgcg gcacgccgtg tgcgccacag gtcagaacgt
    36781 cacaaatgct cggtcctttg ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa
    36841 cggcgccacc ccataagaaa tctttcggta aggtgagttt tttcactatc ataggctcca
    36901 gatcagtcaa ttcttacagg aaatagtacc atgcagccaa acgacatcac ttttttcaac
    36961 gtttccagga tgacattctg gctgggcgta aaaccatcac catccgcgac gagtctgaat
    37021 cgcacttcaa aacgggtgat gtgcttcgtg tcggacgttt tgaagatgac ggttattttt
    37081 gcacgattga agtcaccgca acctcaaccg taacgctgga tacactgacg gaaaaacatg
    37141 cagagcagga aaatatgacc ctgcctgaac tgaaaaaggt cattgctgac atctatcccg
    37201 gtcagacaca attttatatg attgaattta aatgtcttta aattgaactc acgcctgtta
    37261 gttgaaattt cgcatttaat ttcatgattt ttaggagatt agtctcaaat cagaatttat
    37321 tttagctaac aggtgttcac tggaactatt ctcagttacg ctggagaggt atacatgtgc
    37381 gtgtacgttt ctctggagta agttatggtt cagaagcccc tcattaagca gggatattcg
    37441 ctggcagagg aaattgccaa cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt
    37501 gggctggtgt tgctactggt tcaggcggtg gatctcaatg ccagcgccac agcgataacc
    37561 agttacagcc tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac gctctatcac
    37621 gccattcctc atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac
    37681 ctgttgattg ccggaaccta cacgccgttt ttgctggtgg ggctggattc tccgttagcg
    37741 cgcgggttga tgattgttat ctggagccta gcattgctgg ggattctgtt caaactgacc
    37801 atcgcgcacc gattcaaaat tttatctctg gtgacttatc tggcgatggg ctggctgtcg
    37861 ctggtggtaa tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac cttactggcg
    37921 gtaggcggcg tggtttattc gctcggggtg attttctacg tctgcaaacg cattccatac
    37981 aaccatgcca tctggcacgg cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc
    38041 tatttgtata ttgggcaggc gtaatgggta tgcctttgta ggcctgataa gacgcgtcag
    38101 cgtcgcatca ggcatcggag cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta
    38161 cgggcctcgt ccctgttgtg ataaaggcat caaatcccag acatgataag acgcgtcagc
    38221 gtcgcatcag gcgataaatg cctgatgcga tacagagatt tactcttcga gcgaatacgg
    38281 cagcggctcg atatgcagtg tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc
    38341 catatcgtta ttcatgacga cctgtaccac gacctgacca tcttccagtt ttaccgcagc
    38401 cagcaccgta ccggtacgac gccagttctc gcccattttc agctctaagt cttcaccagc
    38461 ttccggcagt cggctggcac tacctgccag caaccagagc gcgcgtttgt tggcaccacg
    38521 gaattttgct cgcgccacca tctcttgtcc ggtataacag cctttcttaa agctgatacc
    38581 gcccagcgcc tggagattgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat
    38641 caccgggaaa ccagcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc
    38701 ttcaccgcgc agtttatcgg tcagcatatt agcagtagct tcatcggtta cgatcaggaa
    38761 acgttctgcc gggtgttcaa accatagcaa agtggtcgcg ccttctttga ctacctgttt
    38821 ttctttcgaa ggcagttcgc taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc
    38881 aacaccaagc agcacacgct cgtcgtctgg cgcgatggtc actttagaga ataccgcata
    38941 ttttttcagt tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc
    39001 atcgccgtcg cggaacagac gtaaattgct ccacatttta cctttggcgt cgcaatgggc
    39061 ggcgagcagg tgctgatctt ctgccatctg gctgacatct gctgtcacct gaccctgcat
    39121 atatttttcg ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag
    39181 cgtcagcggt aaacgggcag aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat
    39241 aatagagtcc tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc
    39301 aatgcaagtg ctttcactac gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga
    39361 agcccttgaa ttgttgtgaa ttaatttcgc gatcgcgatg gtgtttactg atattcccgt
    39421 aaaagaagtc gaaaaacacg ttacaatagc cgggtactgg attttcgtga gacaggaaga
    39481 agatggacat taacaacaaa gcccgaattc attgggcatg ccgccgtggt atgcgcgaac
    39541 tcgatatttc aatcatgtcg tttttcgaac atgagtacga cagcttaagc gatgacgaaa
    39601 aacgcatctt tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga
    39661 atcacggtaa accggccgat gcagaactgg aaataatggt ccgactcatc cagacacgga
    39721 accgggaacg tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt
    39781 tccttgctga ttcatgggct ggttgccgct gttattttac tcatgccctg gccgctcagt
    39841 tacaccccgt tatggatggt gttactttcg ctggtggtat ttgattgcgt tcgcagccag
    39901 cggtgtatta atgctcgcca gggggaaatt cgcttgttga tggacgggcg tttgcgttgg
    39961 caagggcagg agtggagcat cgtcaaagca ccgtggatga ttaagagcgg catgatgctg
    40021 cgtttacgtt ctgatggcgg taaacggcaa catttatggc tggcagccga cagcatggac
    40081 gaagccgaat ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga
    40141 tgacggcaaa gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc
    40201 gttgaggatt tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt
    40261 ttcatccagc gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt
    40321 aaattcatat ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt
    40381 gtcatgcagc ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag
    40441 cccatacagc gcaataattt taccttcaag gttcaggtcg tcgagctgat cccagacggc
    40501 ttcccagtct tcctggattt caccaaaatc ccaggtcggg atacccagaa tgagcacatc
    40561 gtactgctcc attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg
    40621 gccgataata tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta
    40681 aaaaagaccc atattcataa cgtaagcttc tcgattctgc tgtggctttt gcagctagtg
    40741 tatcagaatc gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa
    40801 ggggcgcaag tgaaacagga gctggcacgc atcgagcagt ttcttgatgc tctgtggctg
    40861 gaaaaaaatc tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg
    40921 gagtggttgc atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca
    40981 ttactggcag aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt
    41041 gcagtgcgcc gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt
    41101 gcgcatctcg cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag
    41161 gtcgaacgtt tattacaggc gccattaatt gatcagccac tggagctacg cgataaagcc
    41221 atgcttgaag tgttgtatgc taccgggctg cgtgtctctg aactggtcgg gctgacaatg
    41281 agtgatatca gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc
    41341 ctggtgccgt taggggaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt
    41401 ccgtggctgc tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag
    41461 atgacgcgac agaccttctg gcaccgcatt aaacattatg ccgtgctggc gggtatcgac
    41521 agcgaaaagc tgtcaccgca tgtgttgcgt cacgcttttg ccactcattt attaaatcat
    41581 ggtgcggatt tacgcgtggt gcagatgcta ctgggccaca gcgatctttc caccacgcaa
    41641 atttatacgc atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg
    41701 gcgtgatgtc tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg
    41761 ttagcggcgt tttcaggctt tgctcaggct gatgacgcgg caattcaaca aacgttagcc
    41821 aaaatgggca tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt
    41881 ctgactaaca gcggtgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca
    41941 atgtatgacg ttagtggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag
    42001 ttgaatgcgc ttgaaaaaga gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc
    42061 accgtgttta ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac
    42121 tataacgcgc tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc
    42181 gatgcagaga aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat
    42241 gatgtgatgg caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat
    42301 tacgcacttg gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc
    42361 acacttgttc cgggttacca gccgccgaaa gacatgaaag aattcctcga cgaacaccaa
    42421 aaaatgacca gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgaag
    42481 tcgatgaaac ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca
    42541 gccggggagt acgcagtgcg caagaactgg aacgcagtgt taaaggtatg ttgccctggc
    42601 agcaactaag cggtgtcgaa aaggccgttg agatccttta caacgctttt cgcgaaggaa
    42661 cgcggattat tgtggtcggt gatttcgacg ccgacggcgc gaccagcacg gctctaagcg
    42721 tgctggcgat gcgctcgctt ggttgcagca atatcgacta cctggtacca aaccgtttcg
    42781 aagacggtta cggcttaagc ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt
    42841 taattgtcac ggtggataac ggtatttcct cccatgcggg agttgagcac gctcgctcgt
    42901 tgggcatccc ggttattgtt accgatcacc atttgccggg cgacacatta cccgcagcgg
    42961 aagcgatcat taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg
    43021 tgggtgtggc gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt
    43081 ttgatgagcg cggcatcgca attcctaacc tggcagaact gctggatctg gtagcactgg
    43141 ggacagtagc ggacgtcgtg ccgctggacg ctaataatcg cattctgacc tggcagggga
    43201 tgagtcgcat ccgtgccgga aagtgccgtc cagggattaa agcgctgctg gaagtggcaa
    43261 atcgcgatgc acaaaaacta gccgccagcg atttaggttt tgcgctggga ccgcgtctca
    43321 atgctgccgg gcgactggat gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca
    43381 tcggcgaagc gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga
    43441 tcgaacaagg aatgcaggtt gaagccctga ccctgtgcga gaaactggag cgcagccgtg
    43501 acacgctacc cggcgggctg gcaatgtatc accccgaatg gcatcagggc gttgtcggta
    43561 ttctggcttc gcgtatcaaa gagcgttttc accgtccggt tatcgccttt gcgccagcag
    43621 gtgacggtac gctgaaaggt tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc
    43681 tggagcgatt agacacactc taccccggca tgatgctcaa gtttggcggt catgcgatgg
    43741 cggcgggttt gtcgctggaa gaggataaat tcgaactctt tcaacaacgg tttggcgaac
    43801 tggttactga gtggctggcc ccttcgctat tgcaaggcga agtggtgtca gacggtccgt
    43861 taagcccggc cgaaatgacc atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg
    43921 ggcagatgtt cccggagccg ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg
    43981 tgggcgaacg tcatttgaag gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg
    44041 gtattgcttt taatgtcgat accgctctct ggccggataa cggcgtgcgc gaagtgcaac
    44101 tggcttataa gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg
    44161 acaatatctg gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa
    44221 tcccgctctt atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa
    44281 ttaatccggt aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt
    44341 atctttgact acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag
    44401 ccggatgtct ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc
    44461 gaagccgttg tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg
    44521 ctggaactgg ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc
    44581 gacgccctgg aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat
    44641 gacagcgccg actgttacct cgatattcag gcggggtctg gcggtacgga agcacaggac
    44701 tgggcgagca tgcttgagcg tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact
    44761 gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc
    44821 tccggcgatt acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt
    44881 aaaagcccgt ttgactccgg cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat
    44941 ccggaagttg atgatgatat tgatatcgaa atcaacccgg cggatctgcg cattgacgtt
    45001 tatcgcgcgt ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt
    45061 acccacatcc cgaccgggat cgtgacccag tgccagaacg accgttccca gcacaagaac
    45121 aaagatcagg ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa
    45181 aatgccgaga aacaggcgat ggaagataac aaatctgaca tcggctgggg cagccagatt
    45241 cgttcttatg tccttgatga ctcccgcatt aaagatctgc gcaccggggt agaaacccgc
    45301 aacacgcagg ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca
    45361 gggttatgag gaaccaacat gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat
    45421 cttaacaatg aactgaaaac gcgtcgtgag aagctggcga acctgcgtga gcagggaatt
    45481 gccttcccga acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac
    45541 ggtaaagaga acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg
    45601 atgacccgcc gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc
    45661 attcagctgt acgttgcccg tgacgatctg ccggaaggcg tttacaacga gcagttcaaa
    45721 aaatgggacc tcggcgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc
    45781 gaactgtcta tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg
    45841 gataaattcc acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc
    45901 atctctaacg atgaatcccg taacaccttt aaagtacgct cgcagatcct ctctggtatt
    45961 cgccagttca tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc
    46021 ccaggcggtg ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg
    46081 tacctgcgta tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt
    46141 gtattcgaaa tcaaccgtaa cttccgtaac gaaggtattt ccgtacgtca taacccagag
    46201 ttcaccatga tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc
    46261 gaatcgctgt tccgtactct ggcacaggat attctcggta agacggaagt gacctacggc
    46321 gacgtgacgc tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag
    46381 aaatatcgcc cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt
    46441 gctgaatcta tcggtatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag
    46501 atcttcgaag aagtggcaga agcacatctg attcagccga ccttcattac tgaatatccg
    46561 gcagaagttt ctccgctggc gcgtcgtaac gacattaacc cggaaatcac agaccgcttt
    46621 gagttcttca tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa
    46681 gatcaggcgc aacgcttcct ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg
    46741 atgttctacg atgaagatta cgttaccgca ctggaacatg gcttaccgcc gacagcaggt
    46801 ctggggattg gcatcgaccg tatggtaatg ctgttcacca acagccatac catccgcgac
    46861 gttattctgt tcccggcgat gcgtccggtg aaataagcat tacgttatgc tcacaacccc
    46921 ggcaaatgtc ggggtttttt atttaagctg ggtaaatgca gataatcgta ttctggcttc
    46981 gcgatttgtc gcctgcatca ccatccacgg actgaacgcc cacggcgtgg catcaatacc
    47041 gcgtaataca tcagctaaat cacaccattg ataatccatc acttcatcat cgttgatctg
    47101 taacgcactg gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgcctct
    47161 cggatcggtg gcgcggtagc gaaagtcagg atagatagat tcaggaggcg taatttccac
    47221 gccaagctca taacggcaac ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg
    47281 gtgcccacaa accgagttag tccacacgcc aggccacgct tttttgctca gtgcgcggcg
    47341 ggtaactaat aattgcccct tggcattaaa cagccaactg gagaacgcga gatgtaagag
    47401 ggtgtctgcc gtgtgtgcgg catacttttc cagcgtaccc gtgggaactc cctgtgcatt
    47461 caataaaatg acgtgttccg tttgcataat ttctcacatg taattctgat cactcatcat
    47521 tatagataat tgaagagtga aatgattaac caagagagcg tttatcgaca accgcccgtt
    47581 ttgagcggat gccgacaggt aaaaaccatt taaaaaagta gcatcatata tttgaagcga
    47641 ctttcaatgg ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac
    47701 aacagactga atatagcgcg cattaaaaaa ttcatattat tctccatgct catttttctt
    47761 cagcttgcgc atcagaagcc acatacgtac tgtcctgact tctaaatctt tatcagagac
    47821 ggattccttc accaggtcag catgatgctg atagccatta aagaagacgt tcagcacaac
    47881 ggcgctaagg gttgcgagca taatgccgct atgtagcagc ggttgcagta cggccggtaa
    47941 cttagaaaag aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac
    48001 aatatagagg ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt
    48061 cgccagtacc atgccgaaca tcactagacc agcgccgccc agcacaaatt gcggaatgga
    48121 ggctaccagc accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga
    48181 aatacacacc cagcggctat gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa
    48241 tgacgtgtgg gggaagctgt taaacgtgcc gcctatcatt gtccctacgc catcgacacg
    48301 cagcccgcaa ataatatcgt gtgaagagag tttacgaccg actatttcac ccagtgccag
    48361 gaacatcccc atcgactcga taaacacgat gattaacacg gcagtcatgg tcagaatgga
    48421 aacgggatcg aaaatcggca taccaaacga catcggggtg acaatcgcaa accacgaggc
    48481 atcatgtagc ccggataaat tgacttcatt catcatccac gaaagtaaaa agccaaatac
    48541 aatccccagt aatacggcga cgttggacat aaatcctttc gcatagcgag taatgagcaa
    48601 gataaaaatt aagacggcaa aggagatacc taaataaacg ggattaccat attgcggatt
    48661 ccctttacct ccagcggccc agtcaatacc cacctgaatg atgctaagcc caatagaagt
    48721 aataaccaca ccggtaacca gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc
    48781 taataatgtg gtgataaaac ccgcggcgat agtggcacca aatatcccca gcaggccgat
    48841 atccgggttc atcccaatgg ctatcattgg tgttacagca gcaaaggtca ccgacataat
    48901 caccggcagg cggatcccca taaagcggcc gataccgata cattgcaata atgtgacgat
    48961 cccgcagcaa aagagatccg agctaatgag catcgcaata gcatcttttg agaggcccag
    49021 tcggtcacca atcataagag gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg
    49081 cagaccgagt atgatcagct ttcctggtga taatatgcgg tcaacctcat cagttgggcg
    49141 gtcttgccca gaagatgagg gaagttggga atctatggcg ctcatcagtg tttctccttt
    49201 cgcccgccag aataaaaagc aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt
    49261 ggagtgccag tttttgcgag gaaggggagt tagagacagg aattgcggag gcaatcacat
    49321 aatcgtgctt aaaccacgaa aatgacaaga gaaactgaca atggaattat ctgtaatttt
    49381 ctcatattga tatttcatta atcattctgg catgtcgcac tctcgcattt aatcgttttt
    49441 atctggatag cgctcttttg atcggcaatg ttgaaataat gaaagcggca tagattgagg
    49501 ggcaataatt tgaactcagt cgcaaaatta ta
//
LOCUS       DAJTYH010000016        48068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTYH010000016 DAJTYH010000000
VERSION     DAJTYH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..223
                     /locus_tag="OO609_000947"
     CDS             <1..223
                     /locus_tag="OO609_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632457.1"
                     /translation="FSSLQSAKEKTTVLQDLRKICTPHASLSDEAWEKLMLSDENNKQ
                     HIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(264..1454)
                     /gene="nepI"
                     /locus_tag="OO609_000948"
     CDS             complement(264..1454)
                     /gene="nepI"
                     /locus_tag="OO609_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6632458.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1665..2117)
                     /locus_tag="OO609_000949"
     CDS             complement(1665..2117)
                     /locus_tag="OO609_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6632459.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2170..3504)
                     /gene="adeQ"
                     /locus_tag="OO609_000950"
                     /gene_synonym="yicO"
     CDS             complement(2170..3504)
                     /gene="adeQ"
                     /locus_tag="OO609_000950"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6632460.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3679..5445
                     /gene="adeD"
                     /locus_tag="OO609_000951"
     CDS             3679..5445
                     /gene="adeD"
                     /locus_tag="OO609_000951"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6632461.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5491..6882)
                     /gene="uhpT"
                     /locus_tag="OO609_000952"
     CDS             complement(5491..6882)
                     /gene="uhpT"
                     /locus_tag="OO609_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6632462.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7020..8339)
                     /gene="uhpC"
                     /locus_tag="OO609_000953"
     CDS             complement(7020..8339)
                     /gene="uhpC"
                     /locus_tag="OO609_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6632463.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8349..9851)
                     /gene="uhpB"
                     /locus_tag="OO609_000954"
     CDS             complement(8349..9851)
                     /gene="uhpB"
                     /locus_tag="OO609_000954"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6632464.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9851..10441)
                     /gene="uhpA"
                     /locus_tag="OO609_000955"
     CDS             complement(9851..10441)
                     /gene="uhpA"
                     /locus_tag="OO609_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6632465.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10516..10806)
                     /gene="ilvN"
                     /locus_tag="OO609_000956"
     CDS             complement(10516..10806)
                     /gene="ilvN"
                     /locus_tag="OO609_000956"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6632466.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10810..12498)
                     /gene="ilvB"
                     /locus_tag="OO609_000957"
     CDS             complement(10810..12498)
                     /gene="ilvB"
                     /locus_tag="OO609_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6632467.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12604..12702)
                     /gene="ivbL"
                     /locus_tag="OO609_000958"
     CDS             complement(12604..12702)
                     /gene="ivbL"
                     /locus_tag="OO609_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6632468.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13267..13356
                     /gene="tisB"
                     /locus_tag="OO609_000959"
     CDS             13267..13356
                     /gene="tisB"
                     /locus_tag="OO609_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6632469.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13636..14820
                     /gene="emrD"
                     /locus_tag="OO609_000960"
     CDS             13636..14820
                     /gene="emrD"
                     /locus_tag="OO609_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6632470.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14828..15325)
                     /locus_tag="OO609_000961"
     CDS             complement(14828..15325)
                     /locus_tag="OO609_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6632471.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15322..15684)
                     /locus_tag="OO609_000962"
     CDS             complement(15322..15684)
                     /locus_tag="OO609_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6632472.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15674..16021)
                     /locus_tag="OO609_000963"
     CDS             complement(15674..16021)
                     /locus_tag="OO609_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6632473.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16130..16543
                     /locus_tag="OO609_000964"
     CDS             16130..16543
                     /locus_tag="OO609_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632474.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16590..18032)
                     /locus_tag="OO609_000965"
                     /pseudo
     CDS             complement(16590..18032)
                     /locus_tag="OO609_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18067..19782)
                     /locus_tag="OO609_000966"
     CDS             complement(18067..19782)
                     /locus_tag="OO609_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6632475.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            19949..20842
                     /locus_tag="OO609_000967"
     CDS             19949..20842
                     /locus_tag="OO609_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6632476.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20839..21653)
                     /locus_tag="OO609_000968"
                     /pseudo
     CDS             complement(20839..21653)
                     /locus_tag="OO609_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21653..23269)
                     /locus_tag="OO609_000969"
     CDS             complement(21653..23269)
                     /locus_tag="OO609_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6632477.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23564..24238
                     /locus_tag="OO609_000970"
     CDS             23564..24238
                     /locus_tag="OO609_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6632478.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24277..25938)
                     /locus_tag="OO609_000971"
     CDS             complement(24277..25938)
                     /locus_tag="OO609_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6632479.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26134..26562)
                     /gene="ibpB"
                     /locus_tag="OO609_000972"
     CDS             complement(26134..26562)
                     /gene="ibpB"
                     /locus_tag="OO609_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6632480.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26674..27087)
                     /gene="ibpA"
                     /locus_tag="OO609_000973"
     CDS             complement(26674..27087)
                     /gene="ibpA"
                     /locus_tag="OO609_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6632481.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27081..27153)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27392..27724
                     /locus_tag="OO609_000974"
     CDS             27392..27724
                     /locus_tag="OO609_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6632482.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27726..28958)
                     /locus_tag="OO609_000975"
     CDS             complement(27726..28958)
                     /locus_tag="OO609_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6632483.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29059..30159
                     /gene="cbrA"
                     /locus_tag="OO609_000976"
     CDS             29059..30159
                     /gene="cbrA"
                     /locus_tag="OO609_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6632484.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30120..31457)
                     /locus_tag="OO609_000977"
     CDS             complement(30120..31457)
                     /locus_tag="OO609_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6632485.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31532..32680)
                     /gene="dgoD"
                     /locus_tag="OO609_000978"
                     /pseudo
     CDS             complement(31532..32680)
                     /gene="dgoD"
                     /locus_tag="OO609_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32677..33294)
                     /gene="dgoA"
                     /locus_tag="OO609_000979"
     CDS             complement(32677..33294)
                     /gene="dgoA"
                     /locus_tag="OO609_000979"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6632486.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33278..34156)
                     /gene="dgoK"
                     /locus_tag="OO609_000980"
     CDS             complement(33278..34156)
                     /gene="dgoK"
                     /locus_tag="OO609_000980"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6632487.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34153..34842)
                     /gene="dgoR"
                     /locus_tag="OO609_000981"
     CDS             complement(34153..34842)
                     /gene="dgoR"
                     /locus_tag="OO609_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6632488.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35120..35776
                     /locus_tag="OO609_000982"
     CDS             35120..35776
                     /locus_tag="OO609_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632489.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(35822..36634)
                     /gene="yidA"
                     /locus_tag="OO609_000983"
     CDS             complement(35822..36634)
                     /gene="yidA"
                     /locus_tag="OO609_000983"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6632490.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36748..37146)
                     /locus_tag="OO609_000984"
     CDS             complement(36748..37146)
                     /locus_tag="OO609_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6632491.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37386..39800)
                     /gene="gyrB"
                     /locus_tag="OO609_000985"
     CDS             complement(37386..39800)
                     /gene="gyrB"
                     /locus_tag="OO609_000985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6632492.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(39829..40902)
                     /gene="recF"
                     /locus_tag="OO609_000986"
     CDS             complement(39829..40902)
                     /gene="recF"
                     /locus_tag="OO609_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6632493.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(40902..42002)
                     /gene="dnaN"
                     /locus_tag="OO609_000987"
     CDS             complement(40902..42002)
                     /gene="dnaN"
                     /locus_tag="OO609_000987"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6632494.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42007..43410)
                     /gene="dnaA"
                     /locus_tag="OO609_000988"
     CDS             complement(42007..43410)
                     /gene="dnaA"
                     /locus_tag="OO609_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6632495.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43707..43787
                     /locus_tag="OO609_000989"
     CDS             43707..43787
                     /locus_tag="OO609_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632496.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44017..44157
                     /gene="rpmH"
                     /locus_tag="OO609_000990"
     CDS             44017..44157
                     /gene="rpmH"
                     /locus_tag="OO609_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6632497.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44174..44533
                     /gene="rnpA"
                     /locus_tag="OO609_000991"
     CDS             44174..44533
                     /gene="rnpA"
                     /locus_tag="OO609_000991"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6632498.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44497..44754
                     /gene="yidD"
                     /locus_tag="OO609_000992"
     CDS             44497..44754
                     /gene="yidD"
                     /locus_tag="OO609_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6632499.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44757..46403
                     /gene="yidC"
                     /locus_tag="OO609_000993"
     CDS             44757..46403
                     /gene="yidC"
                     /locus_tag="OO609_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6632500.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46509..47873
                     /gene="mnmE"
                     /locus_tag="OO609_000994"
     CDS             46509..47873
                     /gene="mnmE"
                     /locus_tag="OO609_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6632501.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11898 a        13010 c        12166 g        10994 t
ORIGIN      
        1 ttttagctca ctgcagtccg caaaagagaa aaccacggta ttacaagatt tacgtaaaat
       61 ttgcacgcca catgcgtcat tatcagatga agcgtgggaa aagttaatgt tgtcagatga
      121 aaacaataaa caacatatac gcgaggccat cgtggcgatg gaacgtaata atcagagcaa
      181 ttattgggaa gcgttgggta aggtagaatg cccggatatg taagaacagc aacaactact
      241 gaccaacgct tctttacctg atatcaggat ttcttcattt tcacctttgc agtaaccaac
      301 aatgcagtca gcaacatcaa tgtgccggac aacatcaacg gcgaagtcag accaatatta
      361 tccagcgcat aaccgccgat tgccgcgcca caggtattag caagctgaat aaccgccacc
      421 tgaatagacc cggctttttc tgcctgatcg gccagcgagc gggtgatcca cgttgaccag
      481 ccgacgggaa ccaatgcaaa agttagcccc cagataatcg ccacgccggt agcaacgatt
      541 ttatcgcttc cccacaacgt cagtaccaac gcactcacag ccagtattaa cggcgcgcct
      601 gctaaggcca gttttaccga acgtttaaga atgaacgacg aaagcgacgt accaataaag
      661 ctggcgatac caaaactcaa cagcaccagc gttaagccat ccacgccgaa tcccgccagg
      721 ttcatataca ccgggcgaat atacgtgaag aaagcaaact gcccggcgaa agacatgaag
      781 atggcgatca tccctgccat cacacccgga cgttgtaata agcggaaagt attttgtttc
      841 tgatgcgagg gttcgcctgg cagtgaaggc aatgatttga taatccagaa aatacacagc
      901 acgcccatca ccgccgccgc attaaagaca ttgcgccaac cgataagctc gcctaaaaaa
      961 ctgcccaacg gcgcggcaat caccagcgca atagaaaccg cgccgaagat caccgacagc
     1021 gccttcggca ccgtacgcgg cggtaccaga cgcatggtca gcgacgccga catcgcccag
     1081 aacccgccca gcgccagccc cagacaggca cgaccgatta aaagcaaact gaatgagtta
     1141 gcaaaggaaa ccagcaagca ggagagcgtc agcaaaacgg caaacaaaat aacaacgtag
     1201 cggcggtcag tggcctgaat tgtctgggta ataaacaaac tggcaaacat tgccacaaag
     1261 gcggtcacgg tcaccgattg cccggcaacc ccttccgaaa tgcctaaatc ctgggccatt
     1321 ggcgtcaaca aactgacggg caaaaactca acgataatca gacaggcgac acaaaacgcc
     1381 accgagaaaa cggctgacca gttcggtcgg gtgatggcat ccgcgccgcg gttttcggca
     1441 ataaattcac tcatggtgtt acccgtggca tgtttcaggg gaaaagccga ccactttaac
     1501 attgaaatgt gtgacgaatt taacgttttt gcaactttta cggtgttgct tgagctggag
     1561 ttaagatcga aacggaacag ggcaaagatt accggatgcg gcgtgaacgt cttatccacc
     1621 cgacaggtca ggcgcggtct gtaggctgat aagacgaaac ggtttcaatg caaccagtga
     1681 acgccctcgg caggctgaaa gaacgcgtta tagcgcatct gaaaagcatt aatatcctgc
     1741 atttcaggtt cggtttcacg cagaaaaccc agcgccagcc tgacctgagc atcggtaatc
     1801 ggcgcggaaa ggcgggcttt ttgtaaaatc cggtgccatt gcagcaggtt ctgttcgcta
     1861 tcatcgacaa tgacaatttg ccagataagc aacacctgcg cggcgttttg caggtgcgac
     1921 tcatccgggc gatgcaaata acgtaaaaat tcgtcaccgg ctgactttcc catttgatcg
     1981 accatcgact ccacgggtac gacatcgata ttcagccgat cgctgagacg gcgaatcgct
     2041 tttctggccc cggatgtcag cacccgaaaa ccaaccacaa acactaccgc cagcgtggcg
     2101 agcattatcc agatcatgag ttctcctgcg taatcagaac tcatgatatc tggaaaccct
     2161 cgccagttac taatccacca gaataatctt caatgcgaac agagctgcga ccaccaccac
     2221 acacaggttc agatcgcgcc agcgcccggt gcacactttc atgatgcagt acgacataaa
     2281 gccgagtgca atcccttcgg tgatcgagaa agtaaagggc atcatcaccg tggtaataaa
     2341 cgcaggcacc gattcggtaa aatcatccca gttaacgcgc gccaggctcg aagtcatcag
     2401 cacgccaaca aagattaacg ctccagcggt tgcgtaagga ggaaccatcg ccaccagcgg
     2461 tgagaagaac ataactaaca ggaacataac gccgaccacg acagccgtta gccccgtgcg
     2521 accaccgact gccacaccag aagtactttc aatataggca gtaacagacg aggtaccgat
     2581 aaatgcgcct gccaccgaac tgacgctatc aacatacagc gccttattca tattggggaa
     2641 tttaccgttg ctatctatta agcccgcttt atcagttaca ccaattaatg ttcctgatga
     2701 atcaaatagg ttgatcagca taaaggagaa aatgataccg gcgagttcaa gtgttaacgc
     2761 gccgctcaaa tctacttcac caatgacgcc gctaatatca ggcggaatgg aatagacgcc
     2821 gctaaaatga acatcaccga aaaataatcc acagcaagac gtcaccacaa tagaaaccag
     2881 caccgcggca tgaaaatgac gtgatgacaa cacggttata ataaaaaacc ctaaaatacc
     2941 taacaacacg ccgtgagaac ttaaatcgcc aatcatcacc agcgtgtctt tattggcgac
     3001 aataacacca gtgtttttta atcccattaa ggcaataaat aatccgattc cactggtgat
     3061 accaatacgt aaacttaatg gaatgttgga gatcatccag taccggatac gaaacagcgt
     3121 gagcaaaaat agcccaattg cgccccagaa tatcgcgccc atcccggtct gccaggagat
     3181 ccccatcgcc cccacgacca cgaacgcaaa gaaggcgttc agccccattg ccggagccag
     3241 cgccacgggt aagttagcaa atatccccat cgcaatactg ccgataccgg caatcaaaca
     3301 ggtggtaaca aacaccactt tcgggtccat ttgtgccgcg ccgaggattt gcgggttcac
     3361 aaaaacaatg tacaccatgg ttaaaaaagt ggtcatcccc gcaatcaatt ctgtgcggac
     3421 ggtggtccca tgctgaggta gtttaaataa tcgcgaaagc gtccctgatt cattactcac
     3481 gtaatcggta ttgtcattat tcattttttt gtccattgga ggagatttaa tcccttgctt
     3541 ttgtaaaaca aatgacaaca acggttcagt gataattatc acatttcaat tgcacattaa
     3601 tagatattct ttaataatct cgcgacgttt ctttatgata aataataatc aaattgataa
     3661 aatcaaaatg agaaaaatat gaataattcc attaaccata aatttcatca cattagccgg
     3721 gctgaatacc aggaattgtt agccgtttcc cgtggcgacg ctgttgccga ttatattatt
     3781 gataatgtct ctattctcga cctgatcaat gccggagaaa tttccggccc aattgtgatt
     3841 aaaggacgtt acattgccgg tgttggcgca gaatacgctg atactccggc tttgcagcgg
     3901 attgatgctc gcggcgcaac ggcggtgcca gggtttattg atgctcacct gcatattgaa
     3961 tccagcatga tgacgccggt cacttttgaa accgctaccc tgccgcgcgg cctgacgacc
     4021 gttatttgcg accctcatga aatcgtcaac gtgatgggag aagccggatt cgcctggttt
     4081 gcccgctgtg ccgaacaggc aaggcaaaac cagtacttac aggtcagctc ttgcgtgccc
     4141 gctctggaag gctgcgatgt taacggtgcc agttttaccc ttgaacagat gctcgcctgg
     4201 cgggaccatc cgcaggttac cggccttgca gaaatgatgg actaccctgg cgtaattaac
     4261 gggcagaatg cgctgctcga taaactcgat gcatttcgct acctgacgct ggacggtcac
     4321 tgtccgggtt tgggtggcaa agaacttaac gcctatattg ctgcgggtat tgaaaactgc
     4381 cacgaaagtt atcagctgga agaaggacgc cggaaattac aactcggcat gtcgttgatg
     4441 atccgcgaag ggtccgctgc cagcaatctc aacgcactgg caccgttgat caacgaattt
     4501 aacagcccgc aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa
     4561 gggcatatcg atgccttaat tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg
     4621 gcatatcgcg tcgccagctg gtcgacggcg cgccactttg gtctgaatca cctcggctta
     4681 ctggcacccg gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg
     4741 cagcaggtac tggtgaaagg cgaaccgatt gatgcgcaaa ccttacaggc ggaagagtcg
     4801 gcgagactgg cgctgtctgc cccgccatat ggcaatacca ttgcccgcca gccagtttcc
     4861 gccagcgact ttgccctgca attcacgccc ggaaaacgct atcgggtcat tgacgtcatc
     4921 cataacgaat tgattacgca ctcccgctcc agcgtctaca gcgaaaatgg ttttgatcgc
     4981 gatgatgtgt gctttattgc cgtacttgag cgttacgggc aacggctggc tccggcctgt
     5041 ggtttgctcg gcggctttgg cctgaatgaa ggcgcgctgg ctgcgacagt cagccatgac
     5101 agccataata ttgtggtgat cggtcgcagt gccgaagaga tggcgctggc ggtcaatcag
     5161 gtgattcagg atggcggcgg gctgtgcgtg gtacgtaacg gccaagtaca aagtcatctg
     5221 ccgttaccca ttgccgggct gatgagcacc gacacggcgc agtcgctggc ggaacaaatt
     5281 gacgccttga aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc gtttattcag
     5341 atggcgtttc tttctctgcc agtgatcccc gcgctaaaac taaccagtca ggggctattt
     5401 gatggcgaga agtttgcctt cactacgctg gaagtcacgg aataataaaa aaagcccggc
     5461 gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt
     5521 ctcgcggcgg attttgcgtt cttccattac cgccactatc gccatcaggc agatacaacc
     5581 aatcgcggcg atatccagcg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg
     5641 cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc
     5701 aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc
     5761 agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc
     5821 cagatagata tattcgttac tggcatgttg atacacaccg agcgtggcga taatcagcgc
     5881 cagcgcgatg caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca
     5941 cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc
     6001 tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg
     6061 taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac
     6121 atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc
     6181 gctgatctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc
     6241 gctgccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa
     6301 catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca cacctgctgc
     6361 gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg
     6421 acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa
     6481 gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact
     6541 ggcactgaag cccagcatac aaatggcaga gaggataagc atgaacggca ggaattgttt
     6601 ggtgtttttg ccgtcggcgt agtatgaaac caacgtttta cccacgccat aagtgatgga
     6661 gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg aaatcatatc
     6721 gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac
     6781 caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag
     6841 gtccagggtc ggcttgcgaa cttggtttag gaaagccagc atgggttact cctgaaatga
     6901 atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     6961 aagaaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat
     7021 cacgcttcac gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg
     7081 gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag cactttcgcc
     7141 agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     7201 gcccctgccg cctctttgtg ggaacactct gccgccgcca taccaattaa catctgcggg
     7261 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     7321 ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     7381 ggcccacggt tgccgttaaa caatttgtcc gagccccaac cggctaccag cgcaccgata
     7441 aatccgccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc
     7501 gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc
     7561 acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc
     7621 tctttacgcg tcaaccctgc cccttcttgt tgttgagcaa tttccagcgc gtcgtgccgc
     7681 cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgtagccg ccagcagaga
     7741 aaaatcccca cgactatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa
     7801 tgcagcgcgc tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg
     7861 ttccataatg cccaccaacc gccgcgctcg gtacgtgaat accaggccgt taacagacgc
     7921 gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag cacggcaaac
     7981 gcccatagcg acgtcgagaa gccaaacaga atgttcatga tgcccgtggc gataagccct
     8041 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
     8101 acgccgtaag taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg
     8161 ccgttagcaa ggatttctgg taccgcggcg ttaaaacttt tccgcgtgaa gtaaaacagc
     8221 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata acgggcatca
     8281 atttcatgtt tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
     8341 atcgaacctt agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
     8401 gaaatgtgta atgtgccgcc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa
     8461 ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac cagcatcaac
     8521 cgttcatcct gctgccagcc ttgcagcgta accgcgctgg catcggcatg tttcacaatg
     8581 ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaatgct
     8641 gattcatcga tacgccattc gagatggctg acaatgccgc gcccttccag ctccatttcc
     8701 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac
     8761 cgtcctaaca aacggcgcac cgcgtcgtaa acgcccagcg acagttgttc gatgagctgc
     8821 ccgctctgct tcacactggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta
     8881 cgaatagcag tgatggtctg accgatatcg tcgtgcagct cacgcgccac gtcgcggcgc
     8941 acgctctctt cggtttctaa caaacgttca gccagatgct gattgcgcgc cagttccttt
     9001 tgtagcgact ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc
     9061 agactttgca ccagcaacga gagtaataaa tccaccagat gatcgcgcca ggtttgactg
     9121 gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ctccttgcca gccatagtgc
     9181 caggccagcg cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtcc
     9241 ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca gaccagatgc
     9301 cgcccgcgcc agttgatggg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta
     9361 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcagacc gccagttaaa
     9421 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
     9481 aacgccgccg ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc acgctgatgg
     9541 cgatagcgcg agatcagcgc tacgggcagt aacgtcagca aactaccgat catcaataac
     9601 ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcaccc
     9661 agcaatacgg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
     9721 ggaaataaca gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccatagg
     9781 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
     9841 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc
     9901 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt
     9961 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac
    10021 gtttggtcag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
    10081 aacagccgcc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt
    10141 tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt
    10201 gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
    10261 agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg
    10321 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
    10381 gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca
    10441 tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt
    10501 tcaagccttg agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga
    10561 ctgattacgc tgcactttca cgacatcttc cagcttagcg atttggctta tcatctgctc
    10621 cagacgctgg tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg
    10681 cagacaaaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt
    10741 cattacgccc ggatggttgc gaacggtgag ctccagaatt acgttgttat gagttgtgtt
    10801 ttgcatggct tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta
    10861 aactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgatgat
    10921 ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa
    10981 tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg
    11041 ctcgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat
    11101 gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag
    11161 gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat
    11221 cgccgcaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg
    11281 gcgattgagc ggataagctt gcgcggtcca catctgatgc tgacccacat cggtggtgat
    11341 aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg
    11401 atcgcacgct ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta ccaactggtg
    11461 ccactctgca cgcggttgcg cttccacccg cgggatcaat tgcgccagca cgtcatcaac
    11521 atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcgc gatcgatatc
    11581 gacatgaata attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc
    11641 aaaacgcgca ccgagcacaa tcaacaaatc cgcctcctgc aaaatatagt tggtactgcg
    11701 cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag
    11761 cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc
    11821 aggcgcattg atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat
    11881 tgtagctgcg tcacgaatgc tttcttcgct aaaggcgggt gcaacggctt tttctgccac
    11941 cgcgggctga gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa tgtctatcca
    12001 caccgggcta gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgtgggag
    12061 ctcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt
    12121 gtccacttct tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca
    12181 aatcagcggg atggagtcca gccgcgcatc agcaatggcg gtcaccaggt tagtcgcacc
    12241 cggcccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc
    12301 gataaacccc gcgccctgtt catggcgagc cagaatatgg cggatttgcg tgctttggct
    12361 taaggcgtcg taaacaggca ggatagaacc gcccggaatg cccgtcacaa tcttaatgcc
    12421 ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt
    12481 tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct
    12541 taaacgaaaa accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag
    12601 tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg
    12661 cgcagttggt agtagttttg cgttgaacat ggaagtagtc attggggacc ttgttggttt
    12721 tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga aaatttttca
    12781 gcaagggaaa attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc
    12841 cgccggagcg aggtttcgtc agccgcctgc ggctggtaac cgcaaagcac actgtattat
    12901 gtcaacactg aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac
    12961 gtatttagtg catagttgag tatcgttcac agtttgcgtt ttgtccaaat attactgttt
    13021 atttatacag taagcttcta taatatcact gtacgcaatg tgttatgcgg gggccgcatc
    13081 gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc
    13141 gtgagcacac tgtgttatgt caacaagcac aacgtttctc cttgagatac cgcgtgcaca
    13201 acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag
    13261 acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg
    13321 caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg
    13381 ggagcgggta accgctcccc ttttatattt agcgtgcggg ttggagtcgg atgcgatgct
    13441 gacgcatctt atccgcccta ccatccctcc cggcaacatt tattgccgct tttgtttaca
    13501 tattctgccg ctaaacaatt ccccattcct ggcgtatatc tggctaacat tcatcaatgt
    13561 gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttattctaat tatttttata
    13621 aaagcatccg tgataatgaa aaggcaaaga aacgtcaatt tgttattgat gttggtatta
    13681 ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg
    13741 cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact
    13801 tacggtgtct cacagctgtt ttatggcccg atatccgacc gtgtcggtcg ccgaccagtg
    13861 atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt
    13921 ttgacggtat tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta
    13981 atggcgcgta ctttgccgcg tgatttatat gaacggacac agttgcgcca tgctaacagc
    14041 ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg
    14101 gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg
    14161 accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc
    14221 ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg
    14281 ctgattggcg gtctggccgg gattgctgcc tttgaagcct gctccggcgt gctgatgggc
    14341 gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggcg
    14401 gcattttttg gggcatggtt tgccgggcgt cctaataaac gcttctcaac gttgatgtgg
    14461 cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc
    14521 gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg
    14581 ctgtttccgc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct
    14641 ggtgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg
    14701 atgttgccgc aaaccggtca gggcagcctg gggttgttga tgaccttaat gggattgttg
    14761 atcgtgctgt gctggctgcc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa
    14821 gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat
    14881 cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa
    14941 gcacgctgtc tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct
    15001 ggcagtcacg gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa
    15061 aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcaaac agggcaatta
    15121 aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa
    15181 attcaagata gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa
    15241 aatcggcagg ttgcagagca tccttgtact ctacaaccag cttatgcctg tcctcttcag
    15301 catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat
    15361 atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc
    15421 acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg
    15481 ggtgtagtgc cagaggatca gcgccacgat ggcgaggatg ccaatcgaaa tccagaataa
    15541 catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag
    15601 catggtacga aaccacgcca gtgatgtgcg ctccggctgc agccctgggt cggcaatgcg
    15661 tctggcttta cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacaacca
    15721 tcaatattaa gctgatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca
    15781 tcgctttttc attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca
    15841 gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt
    15901 gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac gccagaaaag
    15961 tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca
    16021 ttgttgctcc tttgcaattt cactaacgct ctatctttag cggttttttt taagaatttc
    16081 gtccgcccgt ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat
    16141 agcgcaaaat aaaatcagtt cattgggcat gctgttcggt gccatcgctt taatgatggg
    16201 gattatccac ttttcattcg gccctttttc cgcaccaccg ccaacgcttg aaagcatcgt
    16261 ggcggacaaa acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag gcgaaaaaat
    16321 taccactgtg gagaaaaaag aggacatgga tatcgataaa atcctcgatc aaagcggtat
    16381 cgcactggcc attgctgcgc tactttgcgc atttattggc ggtatgcgta aggaaaatcg
    16441 ctggggcatt cgcggcgcgc tggtttttgg tatcggcatc gtgtgcagca ttttgctgat
    16501 attcctgatt ttttccttcc tgaccggcgg ttcgctggtt taagcagcct gaataatgcc
    16561 cgccgggtga tcatcagcgg gcattatcgt cagaatttct gttttttctc ctccaccttc
    16621 actccttgcg tcggtaggcc ggtgtcatag tcgcgtacca ctggcgaata gccatcttgt
    16681 gggcgtgggc gaaacgcccc catccagcgc ggtcgtgcat ctttacgcca cggacgcaga
    16741 ctccattggt aactgcggaa cggatcgcga attttgtcca tgtaatccaa taaggcgtca
    16801 tgcattttgc tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg catttcattt
    16861 gggtcattac ggcgatcgta aagttcatca ctggtgaaga ggttgagcac cagtttaaag
    16921 tcatccgtca cccagcaacg caccggaata aaaccgccaa agctgtcatg ctcaatctcg
    16981 tagcggttaa attccaccat cacgccgcgt ggctctttca cggcaaggat attttccccc
    17041 ggcagaatct ctggtttttc aatatctgcc agcgccatca ttgtcggcag taaatcaata
    17101 tgactgactg gcgtatcgac ctgtcgccgc tccccttgcg gcgaacggat gatcagcgga
    17161 atacgggtga tgtcgtcata catcgccgct cctttactga tcagcttatg tgcgcccatc
    17221 atttcaccgt gatcggaggt ataaatgacc cacgtatttt cacgttgctc tggcgttaag
    17281 gcattgatga cacgtccgat ttggtcatca acaaagtcat tgcaggcaaa atagagcgga
    17341 tggtgataaa gcccgtcatc accgactggc gatggcatcg cctgcgccca taagcggtga
    17401 tgttccggtt tgttcgccag gtcatcctgt gatttctcgc caagttcgta gtaaaaatca
    17461 gcgtatttct ctaaatactc caccggacag gtgaacgggt gatgcggctc atcatacgaa
    17521 accaccatca ggaagggttc ctcggcgcgc acgggctatt gcagaaaatc taccgctcga
    17581 ttgctgatgc ggtgcgccca ggtgaaggtt tcgtcaatat ggttcgcctg taaatcttcg
    17641 acgctgttta ggccattgcg ccacaggctg atctcttttt ccgtcagttc gctaagatag
    17701 ttcgccccat caaaccagta atcagcatcc cactccggcg gacactcgcc agtgccgaaa
    17761 tagtcatgac cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc ggcatcctta
    17821 aagtagcgcc ccatagtgga gatgtttttg cctggcgcga cgttgttggt ccacgggccg
    17881 gactggttag cgtagatacc ggtgaatagt ccggcccgtg caggcgtaca aaccggtgaa
    17941 caagtgtagg cggaattaaa gcgaatacct tccgccgcca gactatcaat attttgcgta
    18001 ttcagcggtt taccgctata gcaaccgacc atattggtgg cctgggtatc ggtcaattgg
    18061 ggcgtttcat aggctatcct tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg
    18121 ccaccagctg tcaaaaatca ggtagacaat cactacggca gcgatgaaat aactgatcat
    18181 cgctaaccag cgcgtaccgt agccgccgaa ttcagccagc ccgacataga cgccaatcat
    18241 cgcgaacagg ataccaattg acgcgatctt gacgtttttc cacggtttca tgtccaccgc
    18301 aaacgcatct ttgaaggtga acggcgtggc gcgcggtttg ataaaaccga tcaccagcat
    18361 cacgaccacg ttgatgcaga acgtacaggc cagcacatag aggaaatgga agtcgaactt
    18421 caccagatag ttgatggtga tgtagctgat tatgccaatc cccatcgcca cttttgccgc
    18481 cagcgccggg atgcgcggaa agaaaaagcc cataatgatg atggtaacca gcggcacgtt
    18541 gtaaatgccg ttgagctgtt tcatccagct atacagcccc tgcggcgcgt tcgcgatcca
    18601 cggcgctacc aaaaccgaaa cgatagcgat aaagaaacca aatttgcgcc cgacggtgac
    18661 cagctgctgc ggctcggcat tctggttaat gatgcgacgg taaataccca tactgaataa
    18721 tgtactggcg ctattcagaa agccgttgaa ggtactgatc accgcaccaa ataacaccgc
    18781 gccgaagaaa ccctccagtg gcactggcag aacgttattg accagcgtcg ggtaggccat
    18841 gtcggctttc ggtagatcct gatacagatg aaacgcaatc aaccctggca gcaccagtac
    18901 cagcgggtcg agcattttca gcaccgccgt taacagcgcc cccttttgcc cttccgccag
    18961 gctttttgac gccagcgtgc gttgcacgat gccctgattt gtacaccagt aaaaggtgtt
    19021 caccagaatt aaaccggtaa atgccgcgcc aatcggcaag ggatcggtcg ggccaccgac
    19081 tgagtttaat ttctcggcgt gaacggtggt aatttgctca atgccctgca taaagctgcc
    19141 cttgcctatc gcgatcaggc caaataccgg caccatcaac ccgccgataa ccagcccaat
    19201 accgttgatg gagtccgcca ctgccattgc gcgcagtccg ccgatcaccg catacaaaat
    19261 tcccgccaga ccaagcaaaa ttaccagtag ccagatagcc gcaccgtgag aaatctgtag
    19321 cgattccccg acgtgaaaca ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg
    19381 cagaaagcag acgccggtgg caatgaggaa gcagaagtcg atgataatac gcgtcgtttt
    19441 atcataacgt tcctccagaa aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg
    19501 taaaaagatt agcgcgagga agatcagcgt caccgctgaa gtcacttccc agcccatcac
    19561 cgacatgccg cttttgtagg cctgcccgga aagaccaacc agttgttccg tggaaaggtt
    19621 ggttaacatt aacgaagcgg caataaccgg cgcttttagt gaacgtccgg caagaaaata
    19681 gccttgttgc gatccggtat ctgttttgcg aaccttccac caggtgatca ccgccaccag
    19741 cagcgtaaaa ccgacaaaac tcaagatttg taacgaattc atctcatagc ccttatttat
    19801 gtttatgtcc cggacgcatg aatataggga gaggtaaaat gcgtcgggtc aggcagtcag
    19861 attcttaaaa tacaaacgtc gtatccctga acggattgtt ttctgttaag ttcaggttgt
    19921 gagcataatt cgccggaggg atattaaaat gaatggaaaa ttgcaaagtt cagatgtaaa
    19981 aaacgaaact ccgtacaata ttcccttatt gattaacgaa aatgtgatct ccagcggaat
    20041 ttctctgatc tcgctctggc atacctacgc cgacgagcat taccgggtga tctggccgcg
    20101 ggacaagaag aaaccgctta tcgccaactc atgggttgcg gtgtataccg tacaaggatg
    20161 tgggaaaatt cttttaaaga atggcgaaca aataactctg catagcaact gtattatatt
    20221 tttaaagcca atggatattc actcttatca ctgtgaaggt ttagtctggg aacagtactg
    20281 gatggaattt acccccacca gtatgatgga tatttccgtt ggtcagcaaa gcgttattta
    20341 taatggcgaa atttataatc aggaactcac cgaagttgct gagttaataa cttcaccaga
    20401 agcaataaaa aataatctgg cagtcgcttt tctgacgaaa attatttatc agtggatttg
    20461 tcttatgtcc gcagatggta aaaaagatcc acaacggcgg caaattgaaa aattaattgc
    20521 cactttacat gccagtctgc aacaacgctg gagcgtagct gatatggctg ccacgatccc
    20581 ctgtagcgaa gcctggttgc gtcgtctgtt tttacgctat accggcaaga cgccgaaaga
    20641 atattacctc gatgcgcgtc tggatctggc gctatcgcta ttaaagcaac aaggaaactc
    20701 ggttggcgaa gtcgctgata cgctcaactt cttcgactcc tttcatttca gcaaagcctt
    20761 taaacataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
    20821 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
    20881 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc
    20941 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
    21001 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
    21061 aggccgtagt agcgcacgcg catctgtttg cgatcttgca gcccaacaat ttgcgccatc
    21121 cggctttcaa taccgattgg catgtcacag atgttgagga ttttcgcatt cgggcgcaga
    21181 cgacgcgtgg cttctgcgac aattgctgcc gggttggagt agttgagcat ctaggcgttt
    21241 ggtgaatatt tttccatata atccaccagt tccaggacgc cgccaatgga acgcatgccg
    21301 tacgctattc cgccgggtcc gcaagtttcc tgaccaacaa caccgtggcg cagtgggatt
    21361 ttttcatcca gttcgcgcat cgggtacttg ccgacgcgga tgtgcgccat gacaaaatca
    21421 acgtcgctga acgccacttc aggatcggtg gtgtaactaa acgcaatgtc cggcgctttt
    21481 tctttaagga tgactttaca ggcttcggca atcacttcct gccgcgcgcc atcgttatca
    21541 taaaatttca gtgcacgaag cgggaaacgg tcctgattcg ccaggagcat caacacgatg
    21601 cctggcgtaa aggtgcttcc accgcctgcg acaaccactg agaatttggt cattatactg
    21661 cctccgtaat ggcaacattt tctgctgatt gatgagaatt aattaagcta tcgagctgtt
    21721 cacgcaattg ggatacatgc agaccaatga tcacctgaat ggcatcgcca ctacggaaga
    21781 cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag cgtttgtgac atgtcatgca
    21841 gtgcaatacg tagacgcgtc gcgcaattgt taatgctgga gatattgccg accccgccca
    21901 gggcttgcag gataccggca gcctgatcca gctctttttt tggctctgca gcggtggttt
    21961 ggcctcgcga ggctttgtat tcggcttttg agtagagttt cacttccgca tcttcacgtc
    22021 ccggcgtgca catgttgaac tgcagaatca gggtgcggaa aaccacgaag tacagcaggg
    22081 taaagcacaa cccaatggcg atttgggtca gcatcatatc cgcgtggttg ctgaacatcg
    22141 ggatccagtt ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg cccaccacac
    22201 caaagagata cattacggtc gacattgagg ccgccagcac ggcgtgtacc gcaaacagca
    22261 acggtgaaat gaacaggaag gtaaattcca gcggttcggt aattcccacc agcatggcgg
    22321 ttaaggttgc gggaatcagc aagcccgcca cttttacccg attttccggt gcggcagtga
    22381 agtacatcgc taaagaaatg cccacggcac caaagatttt tgagttaccg tgcagggcaa
    22441 aaccgccttc cgggaacaac gatttcagtg gctcggcgct cagactgaac tcttgcagat
    22501 gctgcgccca gtacatctga atgccgcctt caacagctgc cggaccaaag ataaacggtc
    22561 cgtagatgaa gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc
    22621 acaccccaag cgcacccgcc gaacgcagga acgcttgcag agattcaatc cccatttgta
    22681 cttttggcca gccgagcaat gtcagccagg cacaggggat catcaccagg aaggcgataa
    22741 tcaccacata agacgtcccc tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca
    22801 gacggttatg cagcgccgtc acaatgccgg aaatgataat tgcgccgata atgctggtat
    22861 cgagggtttt aatcccggcc atcattgtca gaccgctacc tgccaccgcg tcctgagtga
    22921 aatcgacgcc gaagtagctt ccccaggtca ttcccatcgc attgatgaaa tagttccagg
    22981 tcaggaaact caccatcacc gccagacaag cacgtccctg cgcttgctta gcaaggccaa
    23041 tgggtaaacc gacagcaaaa atcagcggca tattacggaa taccgtccaa ccgccctctt
    23101 caataatgtg tacgatttgc gcgaataaac tgttcggatc ggtcagtgat tccccgacaa
    23161 acatcgggtt ttgcagcaag atggcaagac ccaccacaat cccggcgaag ggaaacagca
    23221 gcactggcgt gaacatcgcg ccgccaaagc gttgaatttg actgagcatt tccacatcct
    23281 cttttctcaa ttctgtaagt aggaagcctg attggttatg cggcagacct ggagcgagga
    23341 taggaagatg cgatcggcag aacatgacga cccaatggga ttcgtgatcg ctttcatgct
    23401 tttcgttggg cggtagagat ctacgttttc gcgaaaaagt agacatctgt gagcacaggt
    23461 acacagcatc gaacacatct ttaaaaaaaa gatttttttc aatcgattaa gcagaacttg
    23521 tgggcgcatt acccgtgctt gcaggcaaaa aagagagcta gagatgatct acaaaagcat
    23581 tgcggagcgg ttaagaattc gacttaattc cgcggatttc acgctaaaca gccttcttcc
    23641 cggtgaaaaa aagctggcgg aagagtttgc ggtatcgcgg atgactatcc gtaaagccat
    23701 tgacctgctg gtagcgtggg ggctggtggt ccgccgccac ggcagcggca cttacctggt
    23761 gcgcaaagat gtgctgcatc aaaccgccag cctgaccgga ctggtggagg tgttaaaacg
    23821 gcagggaaaa acggtcacca gccaggtgct gatttttgaa atcatgcctg cgcctccggc
    23881 cattgccagc cagttacgga ttcaaatcaa cgagcagatc tacttctccc gtcgcgttcg
    23941 ttttgtggaa gggaaaccgc taatgctgga agacagctat atgccggtaa aactgttccg
    24001 taacctttcg ctgcaacatc tggaagggtc gaagtttgaa tatattgaac aagagtgcgg
    24061 gattttgatt ggcggtaatt atgaaagcct gatgccggtg ctcgccgata gactgctggc
    24121 gcggcaaatg aaggtagcgg aacacacgcc actgctgcgg atcacctcgt tgtcatatag
    24181 cgagagcggg gagtttttga attattcagt gatgttcaga aatgccagcg aatactaggt
    24241 ggagtaccat ttacggcgac tccacccgga aaaaagttaa ccgatactcc agaagagcac
    24301 cgccagtaat tggggggtga taatgcgcag gaacatcact aacggataga cagtggcgta
    24361 agagagcgcc gccgcaccac tggttggatg aagattatta gcaaacgcca gcgccggagg
    24421 atcggtcatg gaacctgcca gcatcccgca catggtcagg taattcattt tggctaacat
    24481 ccgcgccaga atgccaacag taatcagcgg aacggcggtg atcagggcac cataaccaat
    24541 ccagcttagc ccttcgccat tgaccagggt attcacaaaa tccccaccag atttcagacc
    24601 aacgaccgag aggaacagca cgatccccag ctcccgcagc gcgaggttgg cacttggcgg
    24661 cataaaccag tacagcttgc cgatactgcc gatacgcccg aggatcaacg ccataatcag
    24721 cggaccgccc gccagcccca gtttcaacgc ggccgggaat cctggcacaa agacgggaat
    24781 agaacctaac aatacgccaa gcccgatgcc aataaacact ggcagcatct gaacctgttg
    24841 cagtttttgc tgcgcattcc ccagcacatt ggcaacggca tcaattgcgg acggacgccc
    24901 caccaggttg aggatatcgc cgaactgcag gctgatatcg ccactggcga ccagttcgac
    24961 cccggcacgg ttcaggcgcg agataacaac gtcatagcgt tctttaaagt gcaggtcgcg
    25021 aatacgtttt ccgagcacgt tttcattggt gaccaccaca cgctcgacgc gcaaatcagt
    25081 gcctttcgta gacagcgagg tatcgacctc ctgaccaatc accagttgcg cattatgtaa
    25141 atccgctggc tgccccacca gatgcagcaa atcgcccagt tggataatgg tatctggcga
    25201 aggtactttt agggtttctt ctcgtttcag acgcgagcag ataattttgt cgccgttgag
    25261 aatcggtaca tctttaatgg ctaaatcatg caggttaggg ttctcaacgc gaatattgat
    25321 agtcttgatc agcgcgccgc cattggtgcg tgaagactcg tgctgctgag cttctgtctc
    25381 gacattgacg cggaaaataa cccgcaacat ccacatggtg aacaaaatcc cgcaaatgcc
    25441 gaatggatac gccatcgcgt aactcatccc catctgatcg accatttcca ttggtgtacc
    25501 caggtcgcgc aagatctgct gccctgcccc cagcgctggc gtattggtta ccgcaccgga
    25561 gaaaatcccc agcactaccg gcagtggaat atcaaacagt ttatgcagga tggcggtaac
    25621 cagaccaccg atgatgacga tcagaacagc aaacaggttg aggcgtaatc cggagacgcg
    25681 caatgaggca aagaagcccg gccctacctg aatcccgatg gtataaacga acaggatcag
    25741 gccaaattcc tgaataacat gcagcatatc gctacttaat gtcatccccg cctgagaaac
    25801 aaaatggccg acgatgatcc caccaaacag cacgccgcca atacctaatc ctatgccgcg
    25861 aaatttgacg ttgccgataa acaaaccgac gactgccacc aaagccagaa tactgaccgt
    25921 taatgctata tcactcatcg tccatttctc ttgagataac attttaattg ctacggatta
    25981 tggctgaatc ggtgaagcag atttgcctta tagcgcacat tatcacggga aagaacgtgc
    26041 cgaaatatct taaacagtcg aaatttaacg cattgttgaa gaagaaagac aaaaaaagcc
    26101 ccaccagaat ggcggggcaa agagaatagc tagttagcta tttaacgcgg gacgttcgct
    26161 gatagcgata cgctgcgctg cgatgggttc aggctcatta cgaattaaat caatatgcag
    26221 taaaccgttt acgaaggttg cgccagagac ttccatattt tcagccagcg taaaactcag
    26281 gctaaatggc tgattcataa gcccttgatg cagccatttt ttctcttctt ttggctgctc
    26341 cggcgtgcct tttacgctca ggcgcgtgcc ttccagttga atctctaaat cttcctgacg
    26401 gaaacctgcc agcgcaaggg taatgcggta gtggttatcg tcgcttttct caatgttgta
    26461 cggcgggaag ctctggcttt caccggcgtt ttgcagtgcg ttggccagtt tgtcaaaacc
    26521 gatccattga cgcatcagtg gggataaatc gaagttacgc atagtcattt ctccttctaa
    26581 gaagcgagta agtacctgca aatccgaaga ttcgcatatg ctccctgacg gcgagcatgg
    26641 agatgtcagg ccgcgccagg cggccttagg gaattagttg atttcgatac ggcgcggttt
    26701 tttcgcttcc ggaatcacgc gttcgagatc gatatacagc aaaccattta ccaggttagc
    26761 cccacgaaca tgaatgttct cagctaactg gaatttgcgt tcaaagttgc gttcagcgat
    26821 gccctggtac agataggtgc gctctttttg ttcgtcggcg tgagcacctt tcaccaccag
    26881 cagattatcc tgggcggtaa tttccagttc gctctcagca aaaccagcca cagcgatagc
    26941 aatgcggtaa tggttttcgt ctaccagttc aacgttatac ggagggtagc cgccattact
    27001 ctggctctgg ttgttttcta agtggttaaa caaacggtca aatccaatag cagaacggta
    27061 aagcggggat aaatcaaagt tacgcataat caatagctcc tgaaatcagc gagaatgtaa
    27121 gaccttccac aatggacagg tcaggtagcc agaacaccca tcaggcgcgt cctcatcgac
    27181 tacgatgtaa aatgggtctg gaaatgaact tttcaagcct tatcagcgga cttttttttg
    27241 cagtttatgg tctattgcat agactgaggg ggcagcacga aacgttaaag tgcgatagcc
    27301 gccacagtgc gacgtaatga tgatgttatt ttcagcaagg gatcgctata actcatcagg
    27361 ccaacaccca cgatcaacag atgaatttat tatgataaga aatgtgttgt tagcgttcat
    27421 gatatgcagc ggaatgacat tactcggagg atgctccagc gtgatgtccc ataccggggg
    27481 gaaagaagga acgtatcccg gcacccgcgc cagcgccacc atgattggcg acgatgaaac
    27541 caactggggc actaagtcgc tggcgattct cgatatgccg tttaccgctg taatggacac
    27601 gcttttactg ccgtgggatg tgttccgcaa agacagctcg gtgcgctcgc gcgttgaaaa
    27661 aagcgaagcc aacgcgcaag ccaccaatgc ggttattcca ccggccagaa tgcctgataa
    27721 ctgatttagc gatcagtttc ggtgatccac agctggccac cttccatcca cgccagccat
    27781 tgaccatccg gcgagaagac cacggcgtcc gcagaaggtg gattggcgtg gttttcggtt
    27841 aaatactcaa cctcgccgct ttgcgcatgg gcacaaacaa ttcgattatc cagcacaaag
    27901 cccaaccatt ctcctgacgg atgccagtta aatgcagact gaatatccgt tttgttatgg
    27961 gttaacttgc gcggttcgcc gccctgtggc gagataagcc acagttgcac aatgccgtta
    28021 tcatcacgca ttaaaaacgc gatttgcgta ccctgcggat tacagcgcac ccagtggcgc
    28081 gggacgttga ctaaccccgg ataagcccga tgatgggtaa aggttaaacg tcgctgcacg
    28141 acgccacgcg gtggcgcggg tagggttgtt tccgttccac ttaacggcgc atcacctgcc
    28201 gatttccagc tagcttcatc ttgcggtaac tcaacgataa atagctccgg cactttctcg
    28261 ccctttggcg aaagtgtgtc gccaataaac gccagcgcgt gatttcctac ccatccttct
    28321 tcataagcac gattgatttc atcactgcca ggctgcggcg tgggcgtggt tttactcacc
    28381 agcacacacc agtggctacc gctgtattca cgcggatgtt gcttttgtac gttgaccggg
    28441 ccaaacggtg cagcaacccc gacgtttcgc aaatccagcg ccggatcgag ttgatgcatt
    28501 acatggtcgt tataggtaaa gctccccctt tcaccgttcg ggctaaagac atgcacatgg
    28561 ctgccaccgc gcagcgctcc tggcgtatac ggcgcggtaa tatccattgc atcgagattg
    28621 ctcaccttgc cgccttcagc aatgactccg cgtcgatgat ggaagtcgta atgccatgtt
    28681 tcatcaggat tttcagggcc gtgaataaag acatatttct ctgacttcgg atgaacggtc
    28741 accacgccga cgtgtgcgcc ctgtgacgcg cgatagataa cctcgacctc gcctgtatgg
    28801 atattcacac gctcaatggt ctcgccggta aacgacgccc cagaaggacg cacgtcaaat
    28861 accagccact ggctgtcggg cgtccaggta ttggtatttg tattggtatt ggtattggtg
    28921 agcaggtgat tacggggagc aaaggttatc tgtttcatgg cgcggtcctg atacaaaacg
    28981 gttcccgcca tcatacttca caaggacttc actttcagac aatagtcttt tgccatcgtg
    29041 atgaatgtga gtgaaaagat ggaacatttc gacgtggcga ttattggcct cggcccggca
    29101 gggtcggcgt tggcacgaaa gttagccggc aaaatgcagg tgatcgcgct ggataaaaag
    29161 caccagtgcg gtactgaagg tttcagcaaa ccttgcggcg gtctgctggc accggacgcg
    29221 cagcgatctt ttattcgcga tggactgacg cttcctgtcg atgtgatcgc caatccacag
    29281 attttcagcg tcaaaactgt cgacgtcgcc gcatcgctca cgcgtaacta ccagcgaagc
    29341 tatatcaata ttaatcgcca tgctttcgac ttgtggatga aatcgctgat ccccgccagc
    29401 gttgaggttt atcacgatag cctgtgccga aaaatctggc gtgaggatga taaatggcat
    29461 gtcatttttc gtgcagacgg ctgggagcaa catattaccg cccgctatct ggtcggtgcc
    29521 gatggcgcaa actcgatggt gcggcgacat ctctacccgg atcatcaaat ccgtaaatat
    29581 gtcgctatcc agcagtggtt cgcagagaaa catccggtgc cgttctactc ctgcatcttt
    29641 gataatgcga taactgactg ttactcatgg agtatcagca aagatggtta ttttatcttt
    29701 ggcggtgcct atccaatgaa agacggtcag acgcgtttca cgacgctgaa agagaaaatg
    29761 agcgcctttc agttccagtt tggcaaagcg gtgaaaagtg agaaatgcac ggtgttgttt
    29821 ccctcacgct ggcaggattt tgtctgtggt aaggacaacg cctttctgat tggtgaagcg
    29881 gcgggattta tcagcgccag ctcgctggag gggattagct atgcgctgga tagcgcagag
    29941 attctgcgtg cggtgttcct gaaacagccg gagaagagca acgccgctta ctggcgcgcc
    30001 acacacaaac tgcgtttaaa actcttcggc aagatagtaa aaagccgatg cctgaccgca
    30061 ccggctttaa gaaagtggat tatgcgcagt ggtgtggcgc atattccaca gttgaaagat
    30121 tatccaacgc gcttcacatc acccaccagc aggatgtaag agagcgcgcc aatcaacgcg
    30181 acggcggaga tataaaccag tgcaggcgcg aaaccgtaac cctgcgccag gtaccccacc
    30241 accagcggaa cggtgatgcc gcccagacca ccggcgaagt taaacacgcc gccggttaaa
    30301 ccaatcaggc gcatcggtgc cagagaagag accagcgacc aggtaatcga agcaaaaccg
    30361 ttgccgaaga atgccagcgc catcaggcac ataatcatca tcggatcgtt ggtgtagtta
    30421 gcgcccataa tgcaggtgga gatcagcaag ccgcagataa tcggcgtttt acgcgcaaag
    30481 cccagtgaaa agcccttacg taccagcaga tccgcgaccc agccagagag caggacgccg
    30541 acaaacgccg cgaggaatgg caccgtggtc ataaagcctg ctttcagcgc cgtgattcct
    30601 ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa accacagtgc agaagccacc
    30661 gcaaattgcc caagataaac gccgatcagt ttacgatgga acaccagttt ccagtctttg
    30721 gctgttaacg gctgacgcgc ctctttcttc accggcgcat caccatccac cagaccgccg
    30781 ccatcacgaa tgtaatccag ttcagctttg ctgatacctt tggtcaggcg cggcggctga
    30841 taaaccttaa accaaatcag cgaccagata atgccgatac caccagtgac aatgaacacc
    30901 cagtgccagc tcaacatctc ctgaatccag atcagcagcg gagtcaaaaa cgccagaccg
    30961 acaaactgac cagacgtata aaaaccaacg gcggaagcgc gttcatgttc cgggaaccag
    31021 ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt cgaaaatacc ggttatcgca
    31081 cgcagaccaa ttaatgacat taatcccgtg gcaaagcctt ggaataaagt cgccactgac
    31141 caaccaaata tcgcaataaa ataagtcacg cgagaaccta cgcgatctaa aaaccaaccg
    31201 ccggggatct ggcatagcgt ataaagccag gcgaaggccg aaaatacata gcccatttcc
    31261 gctttcgtaa tgccgaactc ttcctgaata tgggcggaag ccacggccag gttagcgcgg
    31321 tcaacataac aaatgaccac cgtaataaag atcatcacca gcgtcagata acgccgacgc
    31381 cccggctttg ctgcattaac gggaatatcc atagcgagct ttctccagat tttgggcata
    31441 gcgaagccgc tcaccatgcc ctgtaattta cagagggtta tatttttgta ttgctgtttt
    31501 agtgcccgat gaggggctta cgtggcagga attaccactc tgctacgctg ttatcttcat
    31561 gacgccagag cggattacgc cagtccgggg catttttact gaactcaata actttagctt
    31621 cgtcgatttc cacacctaag cccggtttcg ttaacggttt aaagaagccg ccaaccatgc
    31681 tgaagtcttc tttgtttttc acaaagtcga gtaactccgc gcctttgttg taatgaatgc
    31741 ccatactttg ttactgaagt accgcgttat aggaaacgaa gtcgatatgc aggcaagccg
    31801 ccagtgcaat cggtccgaac ggacagtgcg gcgcaagggt cacatcatag gcttctgcca
    31861 ttccagcaat tttgtagcat tcggtaatac cgcctgcatg ggagagatcc ggttgcagaa
    31921 tcgaaatacc gcctgcctcc agcacgcgtt taaaatcgaa acgtgagaac atgcgctcac
    31981 ccgccgccag tggaatatgc gtttgtgccg ccaatttcgg gtagtattcg gcttgttccg
    32041 ccagcaccgg ctcttcaata aacagcgggc gatacggctc cagttcttta atcagcactt
    32101 tcgccattgg cgcgctgacg cgaccgtgga aatcaagacc aaactcaatc tgattgccaa
    32161 aagcttcacg aatttgtgcc acggtgttga ctgccgcatc taccgcgcgg gagttatcaa
    32221 ttagccccag ttcttcacaa ccgttcagtt tgaaggtatc gaagccgatt tcgcgcaggg
    32281 ttttaatgcc gtcgataaca tccgccggac gatcgccgcc gacccaactg taggctttaa
    32341 ttttgtcgcg aaccaggccg cccatcagtt gccagactgg cgcattcagc actttgcctt
    32401 tgatatccca taacgcctgg tcgatcccgg cgatggcgct catcaggatt ggaccgccac
    32461 gataaaatcc ggcgcgatac atcacttgcc ataagtcgtt gatgcgcgaa ggatcctgac
    32521 caatcaaata gtcacccagc tcgtgaactg ccgcttccac cgtacgggcg cggccttcaa
    32581 tcacgggctc gccccaaccg accacgcctt catcggtttc aattttcagg aacatccagc
    32641 ggggaggtaa acgatacgtg gtaattttgg tgattttcat tgcactgcct ctcgatacgc
    32701 cttaacaaat gctgctgcct gctgcgcggt gcgttctacg gactgcccgg cgcgataaag
    32761 atcgctgcct aagcccgccc caacacaacc tgcgtctatc cactgcgcca ggttttctgg
    32821 cgtcacgccg ccaacggcaa agactgcgat gtccggtggt aataccgctt ttaacgcttt
    32881 gatgtattgc ggaccaaaag ccgatgacgg aaatattttc agcgcctgcg cgcccgcttc
    32941 gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg caaacggtca tgccgtagcc
    33001 caccgcgcgg cggatcactt cactatggat attgggcgta acgatgagct gacagcccat
    33061 cctggcgagc gcatcgacct gttcaggttt cagtaccgta cctgcgccaa tcaacgcctt
    33121 gtcgccatac gcatcaacga tggcgggaat gctctgctcc cattgtgggg aattcagcgg
    33181 gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca acatgcgcca gcgcctcgtc
    33241 gggcgtaatg ccgcgcaaaa tggcgatcag cgggagttta gtttgccact gcatgagcga
    33301 tgctccttat accagcctga aatgccgtgt cgcccgccac cgccgccacg tcgcaaccca
    33361 tcgcctgaaa ggcttgctgg tagcgcgcgg tcagcgatgt tccggcgaca agggtgatgg
    33421 cgtgttgatg ggtcacatag tcgcgcatac tggcaacctc tgcgccaatc aacaaaccag
    33481 agagaaattc gctgacctgt tcgcggggaa gtgttcccag cacatgcgag gcgcgaactt
    33541 caaaaagctg cggcaatatg gcgggcgcat taaggccgcg ctcaaggcca gccgcgaagg
    33601 catcggcaga gttttcctgc ggcggcaaac ctgcgccaat caatgagtga tttaacagta
    33661 aatgatgtaa ttcaccggtc atcacggtgc gaaaatcgtt gatttgctgg ctatcggcct
    33721 gcacccattt gcaatgcgta ccgggcataa cataaagaga ggaaggagcc agagctcgcg
    33781 cgccgatcaa ttgtgtttct tcgccgcgca tcacattgtg gttatcgtca tgagagacgc
    33841 ataatccggg aataatccag atattgtcgc caacagacgt taattgttcg ccaatagacg
    33901 aaaaacgggc aggaacagat aaatacggag caactttcca gccgacattg ctgccgacca
    33961 ttcctgccat caccactggc gtattttctt cacgccagtc ggtcgtgact tctgctaaca
    34021 ctgcagccgg agattttccg ttcaggcgcg tgacgcctgc ttctgattgc ctgctctcca
    34081 ggcagtggtc gccctgataa agccaggcgc gcagattggt cgatccccag tcaattgcga
    34141 tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc gagctggcaa tcatggtaag
    34201 cgccgcctgc tctgccgcat cgccgtcctg atgccgtatc gcatcgaaca gcgccttatg
    34261 ttcctggagc gtttgcggca tgttggcctc atcgcccatc caggttcgtt caaaaaccgc
    34321 ccgctgcagc gaactgatcg caatgctaag ttgctgtaac accgggttat gcaccgactg
    34381 cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac gcttcgcggt cctgattgtt
    34441 ggcaatcatc tcgttcagcg ccgattcaat ctgcgccaga tcgctggagg tcgcgcgctc
    34501 tgctgcccaa cgggcaatcg ccggttccac cagatttcgc acttcgctca tggcactgat
    34561 aagccgtggg tcgtagtcgt tttccagcac ccattgcagt acgtcagtgt cgaggtaatt
    34621 ccactggtta cgcggtgcca caaacgcccc gcgataacgt ttcatttcaa tcagccgctt
    34681 cgccatcagc gaacggaaca cctcacggat gatgttgcgc gaggttgcaa actcttcaca
    34741 gagctccgcc tcagccgcaa gtggcgagcc aggtacgtat ttgccgtgaa cgatctgttt
    34801 acccagcgta atgacaatgc gatcggtttt attgagagtc atggagagtc cttgtgctct
    34861 gtatgttctt ctctacttta ccccgatcga tgcataacgc ggcaactttg tagtaccagc
    34921 gtgatgacgt tcgcgtttgc cgcgcgcgta atgtagtaca aacttatatt gttgcgctac
    34981 aatttagatc aaaaaaagaa caacacataa aaaaatgaca tgcgtcgggc agaaatctga
    35041 aaagggatat caggcgctaa acaggaggga aagaagagta tgctttcaac ggcttagcta
    35101 ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga ttttttaagg ctgccggttt
    35161 attcccactg gcgctgatgc tttcaggctg tatctcgtat gctctggttt cccataccgc
    35221 aaagggtagt tcaggaaagt atcaatcgca gtcagacacc atcactgggc tatcgcaggc
    35281 aaaagatagt aatggaacaa aaggctatgt ttttgtaggg gaatcgctgg attaccttat
    35341 cactgatggt gccgatgaca tcgttaagat gctcaatgat ccagcactta accggcacaa
    35401 tattcaggtt gccgatgacg caagatttgt tttaaatgcg gggaaaaaga aatttaccgg
    35461 cacaatatcg ctttactact actggaataa cgaagaagaa aaggcactgg caacgcatta
    35521 tggttttgcc tgtggtgttc aacactgtac caggtcactg gaaaacctaa aaggcacaat
    35581 ccatgagaaa aataaaaaca tggattactc aaaggtgatg gcgttctacc atccatttaa
    35641 agtgcgattt tatgaatact attcacccag aggcattccg gatggtgttt ccgcagcatt
    35701 actgccagtg actgttacac tggacatcat tactgcaccg ctgcaatttc tggttgtata
    35761 tgcagtaaac caataatcag taagcgggca aacgcgttta tgctgtttgc ccgcccacag
    35821 attaattcag cacatacttc tcaatagcaa acgccacgcc atcttcaagg ttggattttg
    35881 tgacaaagtt cgccacttct ttcaccgaag gaatagcgtt atccatcgcc acaccgacgc
    35941 ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc aatcgccatg atttcttccg
    36001 gtttaatacc taacacgtcg gccagtgatt tcacacccgt acctttgtta acgcgtttat
    36061 cgaggatttc gaggaagtac ggcgcacttt tcagcacggt atatttctct ttcacttcct
    36121 gcggaatacg cgcgatagcc tggtcgagga tggcgggttc atcaatcatc atcactttca
    36181 ggaactgggt attggggtcc attttctccg cttcgcagaa caccagcgga attgtggcaa
    36241 cgaaggattc atgcaccgtg tagtagctga tatcgcggtt agcggtgtac agcgtggtgc
    36301 ggtccagggc gtggaaatga gaaccgactt cgcgagagag tttttccagg aaacgatagt
    36361 cgtcatagct gagagcggtt tgcgccacgg tgctaccatc agcggccttc tgtaccagcg
    36421 cgccgttata agtaatgcag tagtcgcccg gctgttccat atgcagctct ttcaggtagt
    36481 tgtgcacacc tgcatacgga cgacccgtcg ttagcacgac attcacgcca cgggcgcgag
    36541 ctgcggcaat cgcattttta acggcgggtg aaatggtgtg atcgggcagc agaagggtgc
    36601 catccatatc gatagcaatg agtttgatag ccatgagttc ccaaggtaga ttagttcctg
    36661 acccatgcta acgcgattcc gctcaaaaat cagtacaaca cccgagggaa aaggggatgc
    36721 aacgcgggtt gcatgctccc catttgctta gcggaagagt ttccctttca gcagttccat
    36781 gcctgcggaa agcagatcgt tattggcttg tgctgacact tcaccttgcg gtgagagcgc
    36841 atcaataatc ttcggcaatt gctctgccag taaactggaa gccgtactgg tatccacgcc
    36901 aagtttttgc ccgagatcgg acaccgcatt tgtgccgagc gccgactcca gttgctcgcc
    36961 gctaactgat tgatttctct gttgattact caaccaggtt gagagaatgg cccctaagcc
    37021 gccactttgc agtttttcca gcagcacctg aatgccaccc tgctcctcaa cccaacttaa
    37081 aatcgcctga tatttccccg catcgccttt cagaaaggca ccgacaactt catcaaaaag
    37141 ccccatgata atcacctgta aagcgttacg tgttgaccca aaaagtatag atctgcggga
    37201 tgataattgc ggattgcaga aataaaaagg gcggagatga tctccgccct tttcttatag
    37261 cttcttgccg gatgcggcgt gaacgcctta cccggcctac aaaatcatgc aaaatcaata
    37321 tattgcagga gtctcgtagg actaataagc gtagcgcatc aggcacgctc gcatggttaa
    37381 cgccattaaa tatcaatatt cgccgctttc agggcgttct cttcaataaa cgcacgacgc
    37441 ggttctacgg cgtcgcccat cagcgtggtg aacaactggt cagcggcaat cgcatcctta
    37501 acggtaacgc gcagcatacg acggctttcc gggtccatgg tggtttccca cagctgttcc
    37561 gggttcatct cgcccagacc tttataacgc tggatggaga ggccacgacg ggactctttc
    37621 accagccagt ccagcgcctg ctcgaagctg gctaccggct gacgacgctc gccacgttcg
    37681 ataaacgcat cttcttccag caagccacgc agtttctcac ccagcgtgca gatacgacga
    37741 tattcgccac cggtgataaa ctcgtgatcc agcggatagt cagtatccac accgtgggta
    37801 cgcacgcgaa caatcggctc gaacaggttt tgttcggcat tggtgtggac atcaaacttc
    37861 cactggctgc cgtgctgttc tttgtcgttc agttcgctga ccagcgcgtt cacccagcgg
    37921 gtaacggtct gctcatcaga aaggtcagct tccgtcaacg tcggctgata gataagctct
    37981 ttcagcattg ctttcggata acgacgctcc atgcggttga tcattttctg cgtcgcgttg
    38041 tactcagata ccagtttctc taacgcttcg ccagccaatg ccggtgcact ggcgttggtg
    38101 tgcagcgttg cgccgtccag cgcgatagag atctggtact gatccatcgc ttcgtcgtct
    38161 ttaatgtact gttcctgctt gcctttcttc actttgtaca gcggcggctg agcgatgtag
    38221 acgtgaccgc gttcaacgat ttccggcatc tgacgataga agaaggtcaa cagcagcgta
    38281 cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga tgatgctgtg ataacgcagt
    38341 ttgtccgggt tgtactcgtc acgaccgata ccacagccaa gcgcggtgat aagcgtcgcc
    38401 acttcctgag aagagagcat cttatcgaag cgcgctttct cgacgttaag gattttaccc
    38461 ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct gcttcgcaga gccgcccgcg
    38521 gagtcccctt ccaccaggta cagttcggaa agcgccggat cgcgttcctg gcagtctgcc
    38581 agtttgcccg gcaggccagc taagtcgagc gcacctttac ggcgggtcat ttcacgcgcg
    38641 cgacgcgccg cttcacgggc acgggcagca tcgataattt tgccaaccac gattttcgca
    38701 tcagttgggt tttccagcag gtattccgcc agcagttcgt tcatctgctg ttcaaccgcc
    38761 gatttcacct cagaagaaac cagtttgtct ttggtctggg aggagaattt cgggtccggc
    38821 acttttacgg aaacgaccgc aatcaggcct tcacgcgcat cgtcaccggt ggcgctgact
    38881 ttggcttttt tgctgtagcc ttctttgtcc atgtaggcat tcagggtacg ggtcatcgcc
    38941 gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg gaatgttgtt ggtaaagcag
    39001 tagatgtttt cctggaagcc atcgttccac tgcagcgcca cttcaacgcc aataccgtct
    39061 ttttcggtgg agaagtagaa gatatttggg tggatcggcg ttttgttctt gttcagatat
    39121 tcaacgaacg ctttgatgcc gccttcatag tggaagtggt cttctttgcc gtcacgcttg
    39181 tcgcgcagac gaatggaaac gccggagttg aggaacgaca actcacgcag acgtttcgcc
    39241 agaatttcat attcgaactc ggtcacattg gtgaaggttt cgaggctggg ccagaaacgc
    39301 accatggtgc cggttttttc agtctcgccg gtaaccgcca gcggagcctg cggtacaccg
    39361 tgttcgtaga tctgacggtg aattttaccc tcgcgctgaa taaccagctc cagtttttgc
    39421 gacagggcgt ttactaccga aacaccaacg ccgtgcagac cgccggacac tttataggag
    39481 ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga tcacttccgc cgccgatacg
    39541 ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt catcctgtac agagacagag
    39601 ttatcggcgt gaatggtgac gataatttct ttacagtgac ccgcgagcgc ttcgtcgata
    39661 gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg tgccgtcatc cgtgtcgccg
    39721 atatacatac ccgggcgctt acgcaccgca tccagccctt tcaggacttt gatactggag
    39781 gagtcataag aattcgacat caacgtttct cgctcattta tacttgggtt aatccgttat
    39841 tttacccttt tccacggtaa acatcttcga attttcgtcc gacatgtcta taacgtgttc
    39901 agcactgatc gcgctgacaa agacctgtga ttgcgtcgct tttaagcggc tggcaagcaa
    39961 cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct atcaggtaga gacaccgccg
    40021 cccgctttca cgagtgagga actctccttg cgccagacgt aaggcgcaca tcaacagctt
    40081 aagctgccca cgcgataagg tatcttccac cggcgcaccg tcggcgcgaa tgcgtaagtc
    40141 cgctttatgc gggccatgcg cggtgtaggt tagctggcga tcgcgttcaa aattacgttc
    40201 cagcacctca gcgtactctg tctctttctc ccagccgcgc tggaaagaga aagtcagaga
    40261 aaactcaggg agaaattgct tacaggtatc atccatatcg gccgcgatac cggcgctata
    40321 ctccgcgcgc caggtgctga tttgctccgc cagcgggatc agttctttat cccacgggcg
    40381 tagctgttcg taacgagtca cctggcgcag cgccgcattg cgctgcttga gcaatcgctt
    40441 gagattgctc caggcggtga aaaatccagg ttcgttgtga aagcatcccc agtcgaggaa
    40501 tgctcttctg tatttggggc cgccgttgag taaagtaaac ccttctggcg ttatcaactg
    40561 catcggcatc aggtgcgcca gttccgcgac tttatgcccg tccgtaccgt caatacggac
    40621 tttgctgtcg ccctgtttgt ctttggttaa gccaatcgcc gtctcgcgct cttcgccctg
    40681 taatcgcccg tggagaacaa atgcctcctg ctcatggcga atgacgcgac caatctgcaa
    40741 actgcgaaac gcccgaccat ggccgagcgt atagatggct tccagcacgc tggttttgcc
    40801 actgccgttg gcacctacca gaaagttaaa gccgggagat aaagcgagat ccgcggtttc
    40861 aatattgcgg aaatcgcgga tcaacaagcg ggtgagggac attacagtct cattggcatg
    40921 acaacatagg cagccgactg tgatgcggca tcttcaatct gcacgctgga aaccgaatcg
    40981 gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca gaacatccag cacatagctg
    41041 acgttgaagc cgatttccat ctccgcaccg ctataggtaa cgtcgaggat ctcttccgct
    41101 tcttcctgtt ccgggttgtt ggcagtaatt ttcagctggt tttcgctgac atacaggcgc
    41161 acaccgcgga atttctcgtt agagagaatc gccgcgcgag caaacgcctg cttgagcaga
    41221 tcgcagcctg cttccagatg tttgtccggg ttcttcggca gaacgcgacg gtaatccggg
    41281 aagcgaccat ccaccagttt cgaggtgaaa ataaagtcgc caacgtgggc acgaatgtta
    41341 ttgctgccaa tctgcacacg cagcagattg tcgccgccgt cgagcatacg catcagttca
    41401 atcacgcctt tacgcggtac gatcaccgaa tggcttggca aagattgacc aattggcatt
    41461 gaacagaccg ccagacggtg gccgtcggtt gccacggtgc gcagttcttc accttcggtt
    41521 tcaaacagca taccgtttaa gtaatagcga acatcctgat gcgccatcga aaactgggtt
    41581 gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa attcgacttc gctctgccag
    41641 tcatcgaggt tcgggaaatc cgccgctggc agggtagaca gcgaaaaacg gctacgcccg
    41701 gagcgcacca gcatccgttc accttccagc tgcacggcaa tttccgcgcc ttcaggcaga
    41761 ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg tcgctcctgg ctcgtgtggc
    41821 tgaaccagcg caacgcgtgc caccatttcc atctcgagat cagtaccggt cagcgacaac
    41881 gtaccgtcag caacctgtaa cagcagatta ccgagaatcg gtagcgtagg acgaccacct
    41941 aacggaccgc tcacctgttg tagcggtttt aataaatgct cacgttctac ggtaaatttc
    42001 ataggtttac gatgacaatg ttctgattaa atttgaaaaa tcttctttga tatcgtggct
    42061 ctcttcacgc aactgctcga tcttacggca ggcatgaagc accgtcgtgt ggtcacgccc
    42121 accaaacgca tcgccaatct ctggcagact gtggttagtc agctctttcg ctagcgccat
    42181 cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga gatccgcgac
    42241 tttgatcttg tagtactccg ccaccgtctt ctgaatattg tcgatggtga ccagtttttc
    42301 ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag tcgatggtga tcgcccgtcc
    42361 ggtaaagttg gcgttagcaa taacgcggtt cagcgcccct tccagctcac gtacgttaga
    42421 tcgtagacgc ttggcgataa agaacgccac ttcacccggc aaacgaatgt cattttcgtc
    42481 ggcctttttc atcaggatcg ccacacgggt ttccagctct ggcggttcga tcgccacagt
    42541 cagtccccaa ccgaagcggg atttcaaacg atcctcaacg ccgttgatct ctttcggata
    42601 gcgatccgag gtgagaatga tctgttgatt accttccagc agggcgttga aggtgtggaa
    42661 aaactcttcc tgagatcgtt ctttattagc aaaaaactga atatcgtcga tcagcagtgc
    42721 atctacggaa cggtagtagc gtttaaactc ttcgatcgcg ttgttttgca gggctttaac
    42781 catgtcctga acaaagcgct cggagtgcat ataaaccact ttggcattcg gcttgcgcgc
    42841 cataatgccg ttacccaccg catgcagcag gtgagtttta cccagacccg tgccgccata
    42901 aaggaacaac gggttatagg caccgccagg gttatccgcc acctggcaag ccgccgcgcg
    42961 cgccagttgg ttagatttac cttcaacgaa gttatcaaac gtgtgtttga cgtttacgtt
    43021 agaacgatag gtcggttctg ccggggccgg gacgttatcc caacctgagc gcgtagaagg
    43081 cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg gccgcgacgt tgctcgtcac
    43141 tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact tcaaaacgca gctgtggggc
    43201 atccgctccg cagaaactgg ttagcagtcc attgatatta ttaaggtact tgtcccgtac
    43261 ccaatcgagg acaaaacggt ttggcgcgta cagggccagc gtgttatcgc tcagttccgc
    43321 ctgcaatggg cgtatccaca tactgaattc tgtggctggt aactcatcct gcaatcgggc
    43381 aagacactgc tgccaaagcg aaagtgacac ggcggactcc actcgaacaa aagtcgataa
    43441 tgactaaggc tgaaacattc atgattgttg acgtacgtcg aaaagaccct gcttgagggt
    43501 gacgcacgaa ccgctgtctg cggttatatg cccgatcaag atcctgcaaa acgatcggga
    43561 ccgcggatca tagcctaaac tgcgcaagag atcttctgtt tctcacagat tttccctatt
    43621 tatccacagg actttccaga actcgctaag tgtaaacgat cctgccgcga ggcgggcacg
    43681 atttacgccg catattggaa aatttaatga ccatagacaa aaattggctt aatcgatcta
    43741 ataaagatcc aggacgatcc ttgcgcttta cccatcagcc cgtataatcc tccacccggc
    43801 gcgccatgct ggtttccact ggtgtgaggt cgtacatttt ccctgcgaaa aggtgcggaa
    43861 aagcgcggta aataaggaaa gagaattgac tccggtgtgt acaattatta caatccggcc
    43921 tctttaatca cccatggctt cggtgtccat cgtttcattt ttcggcggat atccaataaa
    43981 gccattgaat ttattcaagt ttaggtagaa atcgccatga aacgcacttt tcaaccgtct
    44041 gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta tggctactaa aaatggtcgt
    44101 caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa
    44161 agctaacccc tgagtggtta agctcgcatt tcccagggag ttacgcttgt taactcccag
    44221 tcaattcaca ttcgtcttcc agcagccaca acgggctggc acgccgcaaa ttaccattct
    44281 cggccgcctg aattcgctgg ggcatccccg tatcggtctt acagtcgcca agaaaaacgt
    44341 tcgacgcgcc catgaacgca atcggattaa acgcctgacg cgtgaaagct tccgtctgcg
    44401 ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct
    44461 cgataaccgt gctctctcgg aagcgttgga aaaattatgg cgccgccact gtcgcctggc
    44521 tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc tgattagtcc gctactcggg
    44581 ccgcattgtc gtttcactcc aacctgttca agctacggaa ttgaggcatt gcgcaggttt
    44641 ggagtgataa aaggcagttg gttgacggtg aaacgcgtat taaaatgcca ccctttacac
    44701 cctggtggtg acgatcccgt cccgcccgga ccatttgata ccagagaaca ctaacgatgg
    44761 attcgcaacg caatctttta gtcatcgctt tgctgttcgt gtctttcatg atctggcaag
    44821 cctgggagca ggataaaaac ccgcaacctc aggcccaaca gaccacgcag acaacgacca
    44881 ccgcagcggg tagcgccgcc gaccagggcg taccggccag tggccagggg aaactgatct
    44941 cggttaagac cgacgtgctt gatctgacca tcaacacccg tggtggtgat gttgagcaag
    45001 ctctgctgcc tgcttacccg aaagagctga actctaccca gccgttccag ctgttggaaa
    45061 cttcaccgca gtttatttat caggcacaga gcggtctgac cggtcgtgat ggcccggata
    45121 acccggctaa cggcccgcgt ccgctgtata acgttgaaaa agacgcttat gtgctggctg
    45181 aaggtcaaaa cgaactgcag gtgccgatga cgtataccga cgcggcaggc aacacgttta
    45241 ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga
    45301 acgctggcga gaaaccgctg gaaatctcct cgtttggtca gttgaagcaa tccatcactc
    45361 tgccaccgca tctcgatacc ggaagcagca acttcgcact gcacaccttc cgtggcgcgg
    45421 cgtactccac gcctgacgag aagtatgaga aatacaagtt cgataccatt gccgataacg
    45481 aaaacctgaa catctcttcg aaaggtggtt gggtggcgat gctgcaacag tatttcgcga
    45541 cggcgtggat cccgcataac gacggtacca acaacttcta taccgctaat ctgggtaacg
    45601 gcatcgtcgc tatcggctat aaatctcagc cggtactggt tcagcctggt cagactggcg
    45661 cgatgaacag caccctgtgg gttggcccgg aaatccagga caaaatggca gctgttgctc
    45721 cgcacctgga tctgaccgtt gattacggtt ggttgtggtt catctctcag ccgctgttca
    45781 aactgctgaa atggatccat agctttgtgg gtaactgggg cttctccatt atcatcatca
    45841 cctttatcgt tcgtggcatc atgtacccgc tgaccaaagc gcagtacacc tccatggcga
    45901 agatgcgtat gctgcagccg aagattcagg caatgcgtga gcgtctgggc gatgacaaac
    45961 agcgtatcag tcaggaaatg atggcgctgt acaaagctga gaaggttaac ccgctgggcg
    46021 gctgcttccc gctgctgatc cagatgccaa tcttcctggc gttgtactac atgctgatgg
    46081 gttccgttga actgcgtcag gcaccgtttg cactgtggat ccacgacctg tcggcacagg
    46141 acccgtacta catcctgccg atcctgatgg gcgtaacgat gttcttcatt cagaagatgt
    46201 cgccgaccac tgtgaccgac ccgatgcagc agaagatcat gacctttatg ccggtcatct
    46261 tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtact gtactatatc gtcagcaacc
    46321 tggtaaccat tattcagcag cagctgattt accgtggtct ggaaaaacgt ggcctgcata
    46381 gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct aaaataaggg cggtcagttg
    46441 accgcctttt ttcttttcgt agggcggata agcaccgcgc atccgccaca caaagcaaca
    46501 ggaacatcat gagcgataat gacactatcg tagcccaggc cacgcctccg ggacgtggcg
    46561 gcgttggcat cctgcgcatc tccggcttca aagcccgtga agttgccgaa accgtgctgg
    46621 gtaaactgcc taagccgcgc tacgccgatt atcttccgtt taaagacgcc gacggcagcg
    46681 tgctcgatca ggggattgcg ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg
    46741 tgctggaact gcaaggtcat ggcggtccgg tgatcctcga cctgctgtta aaacgcattc
    46801 tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt ttccgaacgc gcgtttctta
    46861 acgataaact tgacttagcc caggccgagg cgattgccga tcttatcgac gccagttcgg
    46921 aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc attctccgca cgggttaatc
    46981 atctggtaga agccctcacc cacttgcgca tttacgtcga agcggcaatt gatttccctg
    47041 atgaagagat cgatttcctc tccgacggca aaattgaagc ccagcttaat gacgtgatgg
    47101 ccgatctcga tgcagtgcgt gctgaagcac gtcagggtag tttgttgcgc gaagggatga
    47161 aagtagtgat tgccggacgt cctaatgccg gtaaatcgag cctgttaaac gcgctggcgg
    47221 gccgtgaagc ggcaatcgta accgatatcg ccggaaccac gcgtgacgtg ctgcgtgagc
    47281 atatccacat tgacggaatg ccgctgcata tcatcgacac tgccgggcta cgtgaggcca
    47341 gtgacgaagt ggaacgtatt ggtatcgagc gcgcgtggca ggaaattgaa caggccgacc
    47401 gcgtgctgtt tatggtcgat ggcaccacga cagacgccgt tgatccggca gagatctggc
    47461 cggaatttat cgcccgtctg cccgcgaaac tgccgatcac cgtggtacgc aataaagccg
    47521 atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct
    47581 cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca tctcaaacag agcatgggct
    47641 ttgacaccaa tatggaaggc ggcttcctgg cgcgccgtcg ccacttacag gcgctggaac
    47701 aggcagcgga gcatttgcag caaggtaaag cgcaactgtt gggagcctgg gcaggtgaac
    47761 tgctggcgga agagttgcgc ctggcacagc agaacttaag cgaaatcacc ggggaattta
    47821 cttcagacga cctgctgggg cggattttct ccagcttctg tattggtaag taaccgcgct
    47881 tacgaagccg tattctgact gtcagatgcg gcttcgcttc attgttagca ctcctgttat
    47941 gcctcaaccc ttttttaaac attaaaattc ttacgtaatt tataatcttt aaaaaaagca
    48001 tttaatactg ctccccgaac gattgtgatt cgattcacat ttaaacaatt tcagaataga
    48061 caaaaatt
//
LOCUS       DAJTYH010000017        47843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTYH010000017 DAJTYH010000000
VERSION     DAJTYH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(263..880)
                     /gene="tdk"
                     /locus_tag="OO609_000995"
     CDS             complement(263..880)
                     /gene="tdk"
                     /locus_tag="OO609_000995"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6632502.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1485..1898
                     /gene="hns"
                     /locus_tag="OO609_000996"
     CDS             1485..1898
                     /gene="hns"
                     /locus_tag="OO609_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6632503.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2042..2950)
                     /gene="galU"
                     /locus_tag="OO609_000997"
     CDS             complement(2042..2950)
                     /gene="galU"
                     /locus_tag="OO609_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6632504.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3152..4165)
                     /gene="rssB"
                     /locus_tag="OO609_000998"
     CDS             complement(3152..4165)
                     /gene="rssB"
                     /locus_tag="OO609_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6632505.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4257..5162)
                     /gene="rssA"
                     /locus_tag="OO609_000999"
     CDS             complement(4257..5162)
                     /gene="rssA"
                     /locus_tag="OO609_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6632506.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5275..5733
                     /locus_tag="OO609_001000"
     CDS             5275..5733
                     /locus_tag="OO609_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6632507.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5783..6625
                     /gene="purU"
                     /locus_tag="OO609_001001"
     CDS             5783..6625
                     /gene="purU"
                     /locus_tag="OO609_001001"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6632508.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6785..6869
                     /locus_tag="OO609_001002"
     tRNA            6785..6869
                     /locus_tag="OO609_001002"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6819..6821,aa:Tyr,seq:gta)
     gene            6916..7047
                     /locus_tag="OO609_001003"
     ncRNA           6916..7047
                     /ncRNA_class="other"
                     /locus_tag="OO609_001003"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7231..7908)
                     /gene="narI"
                     /locus_tag="OO609_001004"
     CDS             complement(7231..7908)
                     /gene="narI"
                     /locus_tag="OO609_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6632509.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7908..8618)
                     /gene="narJ"
                     /locus_tag="OO609_001005"
     CDS             complement(7908..8618)
                     /gene="narJ"
                     /locus_tag="OO609_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6632510.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8615..10153)
                     /gene="narH"
                     /locus_tag="OO609_001006"
     CDS             complement(8615..10153)
                     /gene="narH"
                     /locus_tag="OO609_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6632511.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10150..13893)
                     /locus_tag="OO609_001007"
     CDS             complement(10150..13893)
                     /locus_tag="OO609_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6632512.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14286..15677)
                     /gene="narK"
                     /locus_tag="OO609_001008"
     CDS             complement(14286..15677)
                     /gene="narK"
                     /locus_tag="OO609_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6632513.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16016..17812
                     /gene="narX"
                     /locus_tag="OO609_001009"
     CDS             16016..17812
                     /gene="narX"
                     /locus_tag="OO609_001009"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6632514.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17805..18455
                     /gene="narL"
                     /locus_tag="OO609_001010"
     CDS             17805..18455
                     /gene="narL"
                     /locus_tag="OO609_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6632515.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18456..19850)
                     /gene="ychO"
                     /locus_tag="OO609_001011"
     CDS             complement(18456..19850)
                     /gene="ychO"
                     /locus_tag="OO609_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6632516.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20035..20388
                     /gene="ychN"
                     /locus_tag="OO609_001012"
     CDS             20035..20388
                     /gene="ychN"
                     /locus_tag="OO609_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6632517.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20432..21127)
                     /gene="chaC"
                     /locus_tag="OO609_001013"
     CDS             complement(20432..21127)
                     /gene="chaC"
                     /locus_tag="OO609_001013"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6632518.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21285..21515)
                     /gene="chaB"
                     /locus_tag="OO609_001014"
     CDS             complement(21285..21515)
                     /gene="chaB"
                     /locus_tag="OO609_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6632519.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21785..22885
                     /gene="chaA"
                     /locus_tag="OO609_001015"
     CDS             21785..22885
                     /gene="chaA"
                     /locus_tag="OO609_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6632520.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23290..23391
                     /locus_tag="OO609_001016"
     CDS             23290..23391
                     /locus_tag="OO609_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6632521.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRR"
     gene            complement(23535..24389)
                     /gene="kdsA"
                     /locus_tag="OO609_001017"
     CDS             complement(23535..24389)
                     /gene="kdsA"
                     /locus_tag="OO609_001017"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6632522.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24425..25234)
                     /locus_tag="OO609_001018"
     CDS             complement(24425..25234)
                     /locus_tag="OO609_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6632523.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25238..25630)
                     /locus_tag="OO609_001019"
     CDS             complement(25238..25630)
                     /locus_tag="OO609_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6632524.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25627..26460)
                     /gene="prmC"
                     /locus_tag="OO609_001020"
     CDS             complement(25627..26460)
                     /gene="prmC"
                     /locus_tag="OO609_001020"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6632525.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26460..27542)
                     /gene="prfA"
                     /locus_tag="OO609_001021"
     CDS             complement(26460..27542)
                     /gene="prfA"
                     /locus_tag="OO609_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6632526.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27584..28840)
                     /gene="hemA"
                     /locus_tag="OO609_001022"
     CDS             complement(27584..28840)
                     /gene="hemA"
                     /locus_tag="OO609_001022"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6632527.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29054..29677
                     /gene="lolB"
                     /locus_tag="OO609_001023"
     CDS             29054..29677
                     /gene="lolB"
                     /locus_tag="OO609_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6632528.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29677..30528
                     /gene="ispE"
                     /locus_tag="OO609_001024"
     CDS             29677..30528
                     /gene="ispE"
                     /locus_tag="OO609_001024"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6632529.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30679..31626
                     /gene="prs"
                     /locus_tag="OO609_001025"
     CDS             30679..31626
                     /gene="prs"
                     /locus_tag="OO609_001025"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6632530.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31751..33430
                     /gene="dauA"
                     /locus_tag="OO609_001026"
     CDS             31751..33430
                     /gene="dauA"
                     /locus_tag="OO609_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6632531.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33485..33763)
                     /gene="ychH"
                     /locus_tag="OO609_001027"
     CDS             complement(33485..33763)
                     /gene="ychH"
                     /locus_tag="OO609_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6632532.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34041..34625
                     /gene="pth"
                     /locus_tag="OO609_001028"
     CDS             34041..34625
                     /gene="pth"
                     /locus_tag="OO609_001028"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6632533.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34742..35833
                     /gene="ychF"
                     /locus_tag="OO609_001029"
     CDS             34742..35833
                     /gene="ychF"
                     /locus_tag="OO609_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6632534.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36677..39544
                     /locus_tag="OO609_001030"
     CDS             36677..39544
                     /locus_tag="OO609_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6632535.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39644..41563)
                     /gene="dhaR"
                     /locus_tag="OO609_001031"
     CDS             complement(39644..41563)
                     /gene="dhaR"
                     /locus_tag="OO609_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6632536.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41791..42861
                     /gene="dhaK"
                     /locus_tag="OO609_001032"
     CDS             41791..42861
                     /gene="dhaK"
                     /locus_tag="OO609_001032"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6632537.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42872..43504
                     /gene="dhaL"
                     /locus_tag="OO609_001033"
     CDS             42872..43504
                     /gene="dhaL"
                     /locus_tag="OO609_001033"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6632538.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43515..44933
                     /gene="dhaM"
                     /locus_tag="OO609_001034"
     CDS             43515..44933
                     /gene="dhaM"
                     /locus_tag="OO609_001034"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6632539.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45253..46950
                     /gene="treA"
                     /locus_tag="OO609_001035"
     CDS             45253..46950
                     /gene="treA"
                     /locus_tag="OO609_001035"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6632540.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47029..47468)
                     /locus_tag="OO609_001036"
                     /pseudo
     CDS             complement(47029..47468)
                     /locus_tag="OO609_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12008 a        12313 c        11713 g        11809 t
ORIGIN      
        1 ggccgttttt ttgtttaccc aaagcaactt ttccataaac cgacagcatt agccttcatc
       61 atatttgcga cgatgtataa cgcctaaaca cagggatatt gtactttaca ggtcacaagt
      121 caacgtcggt gcttaagagc cctgtgaggc gtatagcggc gttaaaaaac tgctgagaag
      181 ggtatatagc ccggaagaag tgcgtaaaac gaactgacag gataaaagtg ccctgctcac
      241 cctgtcagta aagaaattct tattaatcgt ggcgatgcct ttcctgaata gccgttaatg
      301 agccgacttg taacgcctct ttatagtgtt tacggcatac agaaacgtat cgttcattac
      361 caccaattac cacctgctca ccttcgttat aaggtctgcc tgcttgatca agacgcagca
      421 ccatgcttgc tttacggcca caaaaacaga tggtttttaa ttcaaccagt ttgtcggacc
      481 atgccagtaa gtattggctg ccaataaata attcacctcg aaaatcggta cgtaaaccat
      541 aacaaagtac gggtatatcg agttgatcga caacctccga taattcatat acttgttgtc
      601 tggttaaaaa ctggcattca tcaaccagta cgcaatgaat tgcctgctgt tcatgttccg
      661 cacgaatctc atcaaataat gatgaatttt ggttaaataa ttttgcaggc gatgacaaac
      721 ctatacgcga actgactttc ccggcaccaa agcgatcatc aatttctgcc gtatatacga
      781 cagtgcgcat gccgcgttcc tggtaattgt atgaagattg caacaatgct gtagacttac
      841 ccgcattcat tgcggaatag tagaaatata gctgtgccat cagccacagg ccctcaatga
      901 tatgaaataa agttggctgg agtttatcat aattcgtcag tttttcagct caacagtatg
      961 cacagaagtg ggtatatgcg ttctccctta cgaagccttg cataatcctt ctgagctatc
     1021 attacaactg cctcgcttgt tataagcggg taaatgactg ctggtaacta ttcacaatct
     1081 ttaacctgtt gcgcaagtaa tagccctctg ttgacctcca ggagatagtg caatactaag
     1141 tccatgctct tattgcgact gttctacttt tcatcattcg cttaataggg aattctcgta
     1201 aacacaacta atacagaaga ctgaaaggtc gtcagcctac gataatctcc ccataaaatg
     1261 tgacatgaat caggaagttt taacctcacg tgctgcgaaa tcatcggtgt aaatagggct
     1321 atatgccgcg tcttttctgg ctaattttat gaaaagatat ttattggcgg cacaaaataa
     1381 agaacaattt tgaattcctt acattcctgg ctattgcaca actgaattta aggctctatt
     1441 attacctcaa caaaccaccc caatataagt ttgagattac tacaatgagc gaagcactta
     1501 aaattctgaa caacatccgt actcttcgtg cgcaggcaag agaatgtaca cttgaaacgc
     1561 tggaagaaat gctggaaaaa ttagaagttg ttgttaacga acgtcgcgaa gaagaaagcg
     1621 cggctgctgc tgaagttgaa gagcgcactc gtaagctgca gcaatatcgc gaaatgctga
     1681 tcgctgacgg tattgacccg aacgaactgc tgaatagcct tgctgccgtt aaatctggca
     1741 ccaaagctaa acgtgctcag cgtccggcaa aatatagcta cgttgacgaa aacggcgaaa
     1801 ctaaaacctg gactggccaa ggccgtactc cagctgtaat caaaaaagca atggatgagc
     1861 aaggtaaatc cctcgacgat ttcctgatca agcaataatc ttttgtagat tgcacttgct
     1921 taaaatcccg ccagcggcgg gattttttat tgtccggttt aagacaattt aataagaata
     1981 agctataaaa aaacggcgtc gattgctcaa cgccgtttcg tggataacac cgatacggat
     2041 gttacttctt aatgcccatc tcttcttcaa gccaggcttt aaattccgtg ccaagggtgt
     2101 tatgacgaat accgtattca acgaaggcct gcatgtaacc taatttatta ccgcagtcat
     2161 ggctcttccc tttcatatga taggcttcca ccgtttcttt ttcgatcagc atatcaattg
     2221 cgtcggtgag ctgaatttca tcaccagctc ccggaggggt ttttgccagc aacggccaaa
     2281 tatccgcgct aagtacgtaa cgacccacaa tagcgagatt agacggcgca acatccgctt
     2341 tcggtttttc taccacacca accatcggta cgctttcacc cggcgctaat tcaacgcctt
     2401 tgcaatccac aacgccatat gcggtcacat cagcaaccgg ttcaaccatg atctggctat
     2461 gacccgtttc atcaaagcgg cggatcatct ctgccaggtt atcctgtgac aaatcggatt
     2521 catattcatc cagaataaca tcaggcaaaa taacagctac cggttcatca cccactaccg
     2581 ggtgagcaca caataccgcg tgtcccaggc ctttcgccag accctgacga acttgcataa
     2641 tagtcacgtg cggtggacaa atagactgca cttcatcaag cagttgacgt tttacacgtt
     2701 tttccagcat tgcttccagt tcaaaactgg tatcaaagtg gttttcaata gagtttttag
     2761 atgagtgtgt aaccagcaca atttcagtaa tgccagccgc aatacattca ttcacgacgt
     2821 attgaattaa tggcttatcg acaagtggca gcatctcttt cgggatggct ttcgtcgccg
     2881 gcaacatcct ggttcctaat cccgcaacgg ggataacggc ttttttgact ttcgtattaa
     2941 tggcagccat ttaaattctc ctggactgtt cgtgttttga acgtgttcat atttctgtat
     3001 cgcatcccag tatatcagca ccccgaaggg gctaagttct gaaaaggagg cgagatagca
     3061 taattaagac aattacttat acgaatggcg acgatagtac caccagccga cattagcagg
     3121 taatgcaaat ttagcccgcg ttatcgtttg ctcattctgc agacaacatc aagcgcagtc
     3181 gaccaccggt tccccatatt tggcattgcc aggcatcgca tcgctggctc aattgattca
     3241 aataagcgtt acctaaagtg cctaacggaa caccattact gatttgcacc tggtgttcgc
     3301 cggtatttaa cgtcgcattc agacccgcag aaaccagaat gagatttttc agttcgcgat
     3361 gataatagcc aactaatagc ggaaactgcc ccggcagatt ggcctgacga agtaaatggt
     3421 ttacctgctt caataacgcg cccaactctg gcaaccgttg attttggtgt gcaagctgtt
     3481 cctgtaataa tccgttaaac aatgcgcgta ataacaaggc agcaagtacg ccattatgtc
     3541 cagctcgggt gacatcaagg caataaaatg ccagatcgtt ttccgaaagt gcggcaatat
     3601 caagcaccag gccgggtttg tccgcggcaa ccaattgacg gtaattaacc cggcaatggg
     3661 aaatcacctg ctgaaccggc ggttgtagtt cctgtaataa tttcgccgct gcggcagggt
     3721 tatcaaccat tgcatcccag tcgcgaaaaa gcctttcctc ttcctcaacg cgcgaattaa
     3781 acatgctggg atagagacag gcaaaaacca tctcgcgcaa gcgattcaga tctttaactg
     3841 gtttcagcaa aacatcttca acgcccagac gtaacgcttt ggcaatatct gccatatttt
     3901 cagtggcaga tatcaccaga actggggtct ggtcgcctct gttacgtata tgctccagca
     3961 gtttaagccc gttcattcgt ggcatcgcga tatcacatat catcaggtct ggagtgaaac
     4021 ctcccagcaa ctcaagggca tccaccccat cagccgccag taccgttgtc gctcccaatg
     4081 aggaaaacca tgaatccaga agcgagcgaa atacctgctc atcttcaaca atgagaatct
     4141 gttttccgac caatggctgc gtcatgttct ctcccctgac tggctttact caatagtggc
     4201 atgctattgc cacctgcgcc tgtcagaatt aacttaagtg tagataaaaa ttctggtcaa
     4261 atgttggtgc gtaccaacgg caaaagttcg tccattttcc tttccactgc cagctgtccg
     4321 gccgcaatgg cagcgtgcgc gcgatggaaa tcaagcgtag atatttgcgg gcaaacaggt
     4381 tgaatcagaa tatcgggcgg atcacctgcc atgcggttcc ttttaaggcg gttctccaac
     4441 acctggatag acgtggtcat aatctctgtt gccgttggcg ctgtcacagc ccgacgtgtc
     4501 gttatgctgc ctaacctttc tttcagacgc gcatgccacg gtagagaatc accattctcg
     4561 ctattttctt cactgacatt aaaggagagc agatcttgtt gcatcaaatg agcatcgtgc
     4621 tgcaggtcga ccgctatcac aatatcagcc cccaatgcac gcgtaagaga aataggaatt
     4681 gggttaacta ctgccccatc aaccagccag tagccgttat gtgcaacggg tgccatgagt
     4741 cctggaatac tgcatgatgc gcgaatagca agatggagat cgccttcagt aaaccataat
     4801 tcacgccccg tacttaaatt ggtggcaaca gccgcaaagc gacgggaaca attttcgatc
     4861 tctgtttccg gcattatttc gcgatattga ttgaagacac gctcgccgcg cagtaaccca
     4921 ccgcgctgcc aggagaggtc catcaggcgt aaaacgtccc aataactgaa agaggttacc
     4981 caatcttcca gcgcagataa tcgatcgcat gcataggccg cgcccaccag cgaaccaatt
     5041 gaacatcctg caacgatatc aatttcaata cccacttttt ttagcgcatt aataacgcca
     5101 atatgcgacc aacctctcgc cgcgccagat cccagcgcca gccctatttt tatctttctc
     5161 attattcccg caagattccc ctggaatgca atcgtagcca catcgctact gctcggttaa
     5221 catagtgcta ctcaggcggt taatacgccg ttattgtttc cagggagatt atttgtgtct
     5281 cagctttgtc cctgtggtag tgctgtcgag tatagcctat gttgccaccc ttatgtgtct
     5341 ggtgaaaagg ttgcacctga tccagaacat ctcatgcgtt cgcgttactg cgcttttgtg
     5401 atgcaagacg ctgattattt aataaagacc tggcatccct cttgtggagc agcggcgtta
     5461 cgtgccgaat tgatgaccgg atttgcgcat actgaatggt tgggattgac cgtatttgaa
     5521 cattgttggc aggacgccga caatatcgga tttgtcagtt ttgttgctcg atttaccgaa
     5581 ggtggcaaaa caggtgcaat aattgaacgt tcgcgatttt tgaaagaaaa cggtcaatgg
     5641 tactatattg acggtacacg tccgcagttt ggtcgcaacg atccctgccc ttgtggttca
     5701 ggtaaaaaat ttaaaaagtg ctgcggccaa taatggttga cggtacggtt tagcaaacac
     5761 tctcaacaag gtttttccag caatgcattc actccaacgt aaagttctgc gtactatttg
     5821 tccggaccaa aaaggtctga tcgcacgtat taccaatatt tgctacaagc acgagttaaa
     5881 tatcgtacag aacaatgaat ttgttgatca ccgtaccggg cgctttttta tgcgcacgga
     5941 actggaaggg atttttaatg attccaccct gctggcggat ctcgatagcg cattgccaga
     6001 aggctccgtg cgtgagctga atcctgccgg tcgtcgccgg atagtgattc tggtcactaa
     6061 agaagcgcat tgccttggcg atttgttgat gaaagccaat tacggcggtc tggatgtcga
     6121 aatcgcggca gttattggta accacgatac tttacgttct ctggttgagc gttttgatat
     6181 tccgtttgag ctggtaagcc atgaagggtt aagccgcaac gagcacgatc aaaagatggc
     6241 ggacgccatt gatgcttatc aacctgacta tgtggtgctg gcgaagtata tgcgggtatt
     6301 aacgccggaa tttgtggcac gcttcccgaa taaaatcatc aatattcacc attcattcct
     6361 gcctgcgttt atcggcgcac gtccttatca ccaggcctat gaacgtggtg tgaagattat
     6421 tggcgcaacc gctcactatg tgaatgacaa tctggacgaa ggcccaatca tcatgcagga
     6481 cgttattcat gtcgatcata cctacacagc tgaagatatg atgcgcgcag gtcgtgacgt
     6541 cgagaaaaac gtcttaagtc gtgcgctata caaagtactg gcgcaacgcg tctttgttta
     6601 cggtaatcgg acgattattc tttaatcgcc agcaaaaata actggttacc tttaatccgt
     6661 tacggatgaa aattacgcaa ccagttcatt tttctcaacg taacacttta cagcggcgcg
     6721 tcatttgata tgatgcgccc cgcttcccga taagggagca ggctagtaaa aagcattacc
     6781 ccgtggtggg gttcccgagc ggccaaaggg agcagactgt aaatctgccg tcatcgactt
     6841 cgaaggttcg aatccttccc ccaccaccat cactttcaaa agccccggaa ttctcaaacg
     6901 aatctgcaat caaatattct gcccaagcgg ggaaggatga gaagcttcga ccaaggttcg
     6961 acttgagcgc cagcgagaga gcgttgccgc aggcaacgac ccgaagggcg aagcgcgcag
     7021 cgctgagtaa tccttccccc accaccatcg cttccttgaa taatcaaaac ctgttactcc
     7081 gagctaattt tgcccgatgg caccacgttt accgggccta caatatcctc ccctgtaggt
     7141 cggataagcg aagcgcatcc gacaactcac cccgcaccgt gcccataaaa aaaccccgcc
     7201 gaagcagggt ctatgtgaac taaaattcgt ttagtgacga gcgcgcacca gctggtactt
     7261 acgtgtcaga tactccaccg gtacgctcca gatgtgtacc agacgcgaga acgggaacag
     7321 caggaataac gtcatcccca gcaccaggtg cagacggaag ataaacgcca caccatcgag
     7381 gtgttgagaa gcgccaccgt ggaaggtcac caccgactgc gcccagccaa ccagtttcat
     7441 catctcgcta ccgtccatat gctgagcgga gaacggaatg gtcagcaggc ccagcgcgca
     7501 ctggataacg agcagcgaca ggatcaggat atccgctccg gtagtggttg cacgcacgcg
     7561 tgggctgaac atacgacgtt tcagcaaaag cacgccgcca atcagacaca gcacgccgct
     7621 ggcaccacca gcaaacatcg ccattttctg tttcacttca atcggcagcc aggcttcata
     7681 catccagtgc ggcgtcaaca taccgaagaa gtgaccgaca aaaatcccca gaataccgat
     7741 atggaacagg ttcgacgcca ggttcatccc tttgcgatcc agcatctggc tggacgccgc
     7801 gcgccaggtg tactgcccgt agtcataacg cagccagcta ccaatcagga agaccgcccc
     7861 ggctatgtac gggtagatat caaagaagaa catattcagg aattgcatta gtgctgtcct
     7921 ccggtggtga tattcagata ttgcggcgcg acggcaccgg caaagcgacg ctgatgagca
     7981 gtgattgctg aatcaccgca gcctttgtca gcaaagaatt taacctgctc ttcttcccag
     8041 acagcatcca gcgcctgcgg cgtatcatcg cgcgcttcgt cggcaatttt ttccgccact
     8101 ttgtcgctgt cgatagcggt attggccaat ttcagcagca gatcaaacat cacggcataa
     8161 cggctttcac gctgttgcag acgcgcgctc agcaatgcca gaatcggcgc gatatctttc
     8221 aaaccttcca cggcttcgct ttgcggcagc tgcgacaggt actccagata cagcggcaga
     8281 tggtccggta attcgcggct gtttaactgc aagccgtgct gctcgtactg cgccagcagg
     8341 tccaccatcg cctggccgcg gtcacgggat tcaccgtgaa catgttcaaa tagcaacagt
     8401 gacgtggcac ggccacggtc gaacagttcg ctgtactgga cctgggcatc gagcggatcc
     8461 atcgccgtta aatcgcgcag gaaaatgccc agcgcatggg catcttcttt cgacagattt
     8521 ttcgacgcag caatcgcctc aaacatctct tgttgatgct gccataaggc agcatccgga
     8581 tattcaagga gacgcgatac aatcacgagt tcgatcatgg atgcggctcc gttttgctgg
     8641 tcacatcgat ggcatcgata cgacggctgt tgaacagatt gaatttggta tctgagccgt
     8701 ggcagccatc accaaaggta aagccgcagc catttttctc cgggaaggct tcccgtgcca
     8761 gttcacgatg actactcggc accacaaagc gatcttcgta gttagcaatc gccagataac
     8821 ggtacatctc ctgtgcctgg gcttcggtca gaccgacctc ttccagcgca cgggtatcaa
     8881 ctttaccgtc aacagtttca gcacgtttgt aatgacgcat cgccagcata cgtttcagcg
     8941 cacgcagtac cggtttggta tcaccggcgg tcagcagatt cgccagatac tgtaccggaa
     9001 tacgcaagct ttcgacgtct ggcagaattc cgttgctgcc cagctcgccc gcgtctgctg
     9061 cagactgaat cggagacaga ggcggcacgt accagaccat cggcagtgtg cgatattctg
     9121 gatgcagcgg cagcgccagt ttccattcca tcgccatttt ataaaccggc gactgctgtg
     9181 cagcttcaat aacgctcagc ggaataccgt ctttaatcgc ctgctcgatg actttcggat
     9241 cgttcggatc gaggaacacg tccagctgac gctggtagag atctttctcg ttctcggtgc
     9301 tggctgcacg ttcaatcgcg tcggcatcgt acagcagcac gccaagataa cggatacgac
     9361 cgacacaggt ttctgagcac acggtcggct gacccgcttc aatacgcgga tagcagaaga
     9421 tgcacttctc agacttaccg ctcttccagt tgaagtagat ttttttgtac gggcatccgg
     9481 tgatgcacat acgccagcca cggcatttat cctggtcgat caggacgatg ccatcttctt
     9541 cacgcttgta aatcgcaccg ctcgggcagg tcgccacaca tgccgggttc aggcagtgtt
     9601 cgcacaggcg cggcaaatac atcataaagg tgttttcgaa ctggctatac atcgccttct
     9661 ggatgttgtc gaagttcttg tctttcgcca gtttatcaaa ctcaccgccc aggtcatctt
     9721 cccagttcgg ccctttttcg attttcgcca tccgttcccc ggtaatcagc gaacgcggac
     9781 gggcaatcgg ctgcgatttg ctgccttccg gcgcggtatg caggttctga taatcaaaat
     9841 cgaacggctc gtaataatcg tcgatccccg gcagatgcgg gttagcaaag attttaccca
     9901 gcagcatggc tcggttaccc atgcgcggtt gcagtttgcc attgatttta cggatccagc
     9961 cgcctttgta tttttcctgg ttttcccagt cagtcgggaa gccctggccc ggcttggttt
    10021 ccacgttgtt gaaccacgcg tattccacgc cttcacggct ggtccagacg tttttacagg
    10081 taactgaaca ggtgtggcag ccgatgcact tatcgagatt cagcaccatg ccgacttgtg
    10141 aacgaatttt cattttacgc tctcctgtac ctggtcattg ccttcgccat ctaaccagtc
    10201 aatgttcttc atcttacgca ctacaacaaa ctcatcgcgg ttagaaccta cggtgccgta
    10261 gtagttaaag ccgtatgcca gatgggcata gccgccgatc atatgcgtcg gtttcggcgt
    10321 gatacgggtg accgagttat ggataccacc acgctgttgg gtaatttccg aaccaggcag
    10381 gttaacgata cgttcctgcg cgtggtacat catggtcatc cctgccggaa cacgctggct
    10441 gacaaccgca cgggcagtca gtgcaccgtt gctgttgaag acttcaatcc agtcgttatc
    10501 ggcgataccc agatctttgg catcggcttc actcaaccag accaccggac caccgcgacc
    10561 taaagtcagc atcagcaggt tgtcgctgta ggtggagtgg ataccccact tctggtgcgg
    10621 cgtcaggaag ttgagcgctt tttccgggtt gccgttggat ttctggccta tcacttcttt
    10681 caccgaacgg gtgtcgatcg gcggacgata aaccagcagg ctttcaccga aatcacgcat
    10741 ccactggtga tcctgataca gttgctgacg accagagagc gtacgccatg ggatcagctc
    10801 gtgaacgttg gtgtaaccgg cgttgtaaga aacgtgttca tcttccagac cagaccaggt
    10861 cgggctggag ataattttgc gcggctgtgc ctgaatatcg cggaagcgga tcttctcgtc
    10921 ttctttattc agcgccagat gcgtatggtc acgaccggta aattcgctca gggcagccca
    10981 ggctttcacg gctacctgac cgttggtttc cggtgccagc gtcaggatca tctctgccgc
    11041 atcaattgcg gtgttcagca tcggctggcc ttttgccgga ccttccgctt tggtgtagtt
    11101 gagcttacgc agcagatcca tctcgctctg ggtgttccag gcaatccctt taccgccgtt
    11161 accgattttc tccatcagcg ggccgataga ggtaaagcgt tcgtaagttg ccggataatc
    11221 gcgctctacg accataatgt gcggtgcagt tttgcctggg atcaggtcgc attcgccttt
    11281 tttccagtct ttcacatcca gcggctgcgc cagttcagcg gcagagtcgt gctggatagg
    11341 cagcgtgacg atgtcggttt ctttacccag atagccgacg cacacttcgg agaatttctt
    11401 cgcgatggct ttgtagattt cccagtcgct tttcgcttcc caggccggat cgaccgccgc
    11461 agacagcggg tgaataaacg gatgcatatc cgaagtattc atgtcgtctt tttcgtacca
    11521 ggtcgccgtc ggcaaaatga tgtcggaata gagacaggtg ctcgacagac ggaagtccag
    11581 cgtaaccacc agatccagct tgccttccag accattgtcc tgccagtcca cttcttccgg
    11641 cttcacgccg ccctgttgcc ccagatcttt accctggata ccgtgctccg tccccagcag
    11701 gtacttgagc ataaactcat gacctttacc ggaagaaccg agcaggttag aacgccagat
    11761 gaacaggttg cgcgggtggt ttttaccgtt ttctggttgt tctgccgcaa aacggatgga
    11821 accctctttc agggatttca ccgtatagtc caccggattc atcccggctt tttccgcttc
    11881 gccagcgata gtcagcgggt tagtgcctaa ctgcggtgca gacggcagcc agcccatgcg
    11941 ttccgcacgg acgttaaagt cgatcaagtg tccggtatag cgggatttgt ccgccatcgg
    12001 tgacagcaac tcttccgccg tgacggtttc ataacgccac tggctggagt ggttatagaa
    12061 ataagaagtg ctgttcatgt gacgcgccgg acgctgccag tcaagggcaa acgccagcgg
    12121 ctgccagccg gtttgcggac gcagtttttc ctgacctaca tagtgcgccc agccgccccc
    12181 gctctgaccg acacagccgc agaaaatcag catgttgatc agaccacgat agttcatatc
    12241 gaggtgatac cagtggttca gccccgcacc gacgataatc atcgaacgac cgtgcgtttt
    12301 atcagcgtta tcggcaaatt cacgggcgat gcgaataatc tggctgcgag aaacgccggt
    12361 aatctgctcg gcccaggccg gggtataagc tttcacatcg tcatagctgg ttgcacagtt
    12421 aacgtcgttc aggccacgtt ccagaccgta gtttgccagc gtcagatcat aaacggtggt
    12481 caccagggcg gtgctgccat cagccagttg caggcgtttc accggcagtt tgtgcagcag
    12541 cacgttttcc agttccactt tgttgaagtg ttccgtgccg tcgccaccaa agtacgggaa
    12601 gcctacctca gcgatctcat cctggctacc cagcaggctc agttgcagct cggtttcttc
    12661 gccagttttg ccgtcgcgct gctcaagatt ccatttgccc ttctcgcccc agcggaagcc
    12721 aatagaaccg ttcggcgcaa ccatttcgcc attggtatta aaggcgacag ttttccattc
    12781 cggattgttt tcctggccca gcgcatcaac cagatcagca gcgcgcagca tacgacctgc
    12841 agcgtagtaa ccgtcgcgtt cttccagcat caccagcatc ggcatgtcgg tgtagcgacg
    12901 cacatagtcg gtgaaatact ggcttgggtt gtcgaggtgg aattcacgca gcattacgtg
    12961 gcccatcgcc agcgccattg ccgcatcggt gccctgtttc ggtgccagcc acagatcgca
    13021 cagtttggcg atttcagcgt agtctggtgt gacggcaaca gttttggtgc ctttgtaacg
    13081 cacttcagta aagaagtgag catccggggt acgcgtctgc ggcacgtttg aaccccaggc
    13141 gatgatgtag ctggagttat accagtcagc agattccggt acgtcagttt gctcgcccca
    13201 ggtttgcgga gacgcaggag gcaggtcgca gtaccagtcg tagaagctta agcaagtacc
    13261 gccaatcagc gagagatagc gtgcacccga tgcgtaagaa accatcgaca ttgccggaat
    13321 tggcgagaaa ccagcaacac ggtccgggcc gtagtttttg atggtgtaaa cgttagatgc
    13381 ggcgatcagt tcgttcacct cctgccaaga agaacgaaca aatccaccgc gtccacgcgc
    13441 ctgcttaaag cttttcgctt tatcggcgtc ttcaatgata gaagcccatg cctcaaccgg
    13501 atcgctatgc agcgccttcg cttcacgcca cattttcatc aggcgtttgc gcatcatcgg
    13561 gtatttcagg cggttggcac tgtaaagata ccaggagtag ctggcaccgc gcgggcagcc
    13621 gcgaggttca tggtttggca gatccggacg ggtacgcgga tagtcagtct gctgggtttc
    13681 ccaggtgacc agaccgtttt tgacgtagat tttccagctg caggagccgg tgcagtttac
    13741 cccgtgggta gagcggacga ttttgtcatg ctgccaacgc tggcgatatc catcctccca
    13801 gtcacggttg gtattgagaa gctggccatg cccatcggca aaggtttcac ccttctgttt
    13861 gaagtagcga aaccggtcca ggaatttact catcggtttt ctcctgtggg agcctgtcgg
    13921 ccctctgata aatcgacatt gctaatgttt aagggcaagg taacgctctg aaacagcgtg
    13981 ggaattgata acgatcaaga gtgatgggga tgaaaaataa agtaaattcc taagataccc
    14041 ctttaggata attaacagtt acagcataac atgctgtaaa ttaagacgtt ttatacctat
    14101 agttatttaa actattaaga tttttatgca aatgggtatt aaggagtatt ccccatgggt
    14161 aacatattaa tggctcttaa tgaaatccgg cgaaataagc catataacac taatatgttg
    14221 taactaaatg acgcataaaa aaagcgcggc atcatgccgc gcaaaggata atcaaatatt
    14281 aagatttatt ttttagaatg ccgaccatat accgcccaag taatcactac gcaggcgata
    14341 tagaaaatca aaaatacttt cattgcacct actggcgaac ccgttaatgc cagcgagcta
    14401 ccaaacgctt tcgggataaa gaagccacca atcgcgccaa tcgcagagat gaaacccagc
    14461 gccgctgccg tgccggttgc cgcttcacgc atcgcacgtt cgtcagaacc cccttctgct
    14521 ttcacgcgat ccattgtcag tttacggaag atcactgaaa tcatctggaa agtggaacca
    14581 ctacccagcc cagctgtcag gaacagcgcc aggaagaccg cgaagaacgc catgaagctt
    14641 ccgccctgcc cgtcagtcgg taaggtcagg aacagcaggc cgctgaaaat cgccatcaga
    14701 ataaagttca ccagcgtgac acgagttccg cccagacggt cagataatgc accacctgca
    14761 gaacgcgcca gcgcaccaat aaacggcccg aagaaagcgt attgcagaat ctgaacatcc
    14821 gggaactgcg tttttgacag catcgcaaag cccgcggaga agccgatgaa ggagccgaag
    14881 gttgccagat acagcaggct cataatccac agatgacccc gtttgagtac cggcaactgc
    14941 tccttgatgg aggctttcga ggtagcaaga tcgttcatgc caaaccacgc cgcaatggtg
    15001 aagatggcaa ggaacggcac ccatatccag gacgcattcg ccagatacag ctcagtccca
    15061 tccggctgtt tgacgccctg gctaccaaat actgcgaaaa tcgacagtga taccaccagc
    15121 ggagcaacca actgcatgac gctgacgccc atgtttccca gaccaccatt cagacccagc
    15181 gcgccaccct gcttctgttt cggaaagaag aagctgatgt ttgccatact ggatgcgaag
    15241 ttcgcgccag caaagccgca tagcagagag atgatgatga agacgctata aggcgtggag
    15301 gtatcctgca cggcaaaacc cagccagacg caaggaataa tcagaatacc ggtgctgaac
    15361 gccgtccagc gacgaccacc gaagatagga accataaagg agtatggaac acgtaataac
    15421 gcgccagaaa ccgaaggcag cgcagtcaac ataaatagct gatcggtcgt aaaattaaaa
    15481 ccgactttcg gtaagttcac cgcaacagcg ctgaacaaca tccatacgca aaacgccagc
    15541 agcagacagg gaacggaaat ccacaggttg cggctggcaa tacgttgacc gcgttgttgc
    15601 cagaacgcag gatcttccgg tcgccaatct gttatgacag ctccagtagc cctttcggag
    15661 gcagatgagt gactcataga cacctctgat actcgtttcg atgtctgcca ccttagtgtc
    15721 tggagctaaa ggcaatttga tgtaaatcaa acgataagat aactttatct atcattgata
    15781 tttatcatta cccatagtga gtacagtgat ttcataaaaa ttatgagatt tttcacggtg
    15841 ctgtagaatc cctaccctta ccgatgtaaa gcgactaacc acacggcaaa taaggagtaa
    15901 ctctttccgg ctatgggtat acttcagcca atagccgaga atactgccat tccagaatat
    15961 atcgtcacat tcattaaggt tattgctcat ttaaagcctg aaggaagggg tttacatgct
    16021 taaacgttgt ctctctccgc tcaccctggt taatcaggtt gcgcttattg tgttgctttc
    16081 tactgctatt ggactggcag ggatggcggt ttctggctgg ctggtgcaag gcgttcaggg
    16141 cagcgcccat gcgatcaaca aagcgggatc gctgcgcatg caaagttacc gtctgttggc
    16201 ggcagtgcca ttaagcgaga aagacaagcc cttaattaaa gagatggaac aaacggcatt
    16261 tagcgccgag ttgactcgag cagcagaacg agacggacaa ctggcgcaat tacagggctt
    16321 acaagattac tggcgtaatg aactgatccc tgcgctgatg cgtgcacaaa accgagaaac
    16381 ggtgtcagcg gatgtcagcc agtttgttgc cgggcttgat cagctggtat ctggttttga
    16441 ccgcaccacg gaaatgcgta ttgagacagt ggtactggtc catcgggtaa tggcggtatt
    16501 tatggcactt ttactggtgt tcactattat ctggttgcgg gcgcgactgc tacaaccgtg
    16561 gcggcaactg ctggcaatgg cgagtgccgt cagccatcgc gattttaccc aacgcgcaaa
    16621 catcagcggg cgcaacgaaa tggcgatgct tggaactgcg ttgaacaata tgtctgcaga
    16681 actggccgaa agttatgccg tacttgagca gcgggttcag gagaaaaccg ccgggctgga
    16741 gcataaaaat cagatcctct cttttttatg gcaggctaac cgccgtttgc attcccgcgc
    16801 cccgctgtgt gaacgcctgt caccggtact caacggctta cagaatttaa ccctgctacg
    16861 tgatatcgaa ctgcgggtgt atgacactga tgatgaagag aatcatcagg agtttacctg
    16921 ccagccagat atgacttgtg atgataaagg ctgccagctc tgcccgcgcg gcgtattacc
    16981 cgttggtgat cgcggcacga ccctgaagtg gcggctggct gactctcata cgcagtacgg
    17041 tattttgctg gcgaccctgc cgcaggggcg tcatcttagc catgaccaac aacaactggt
    17101 ggataccctg gttgaacaac ttaccgccac gctggcgctg gatcgccatc aggaacgtca
    17161 gcaacagttg atcgtgatgg aagagcgtgc caccattgcg cgcgaactgc atgattctat
    17221 tgcccaatct ctctcttgca tgaagatgca ggtgagttgt ttacagatgc agggcgatgc
    17281 gctgccagaa agcagccgcg aactgttaag tcagatccgt aacgaactga atgcatcctg
    17341 ggcgcagttg cgtgaattgc tcaccacatt ccgcttgcag ctcaccgagc ctggattacg
    17401 tccggcgctg gaagcgagtt gcgaagagta cagcgccaaa tttggcttcc cggtgaagct
    17461 ggattatcaa ttgccgcctc gtctggtgcc ttcacatcag gcaatccact tgttgcaaat
    17521 tgcccgtgag gcattaagta acgccctcaa acattcgcaa gcgagtgagg tcgtggtgac
    17581 ggtggcgcaa aacgataatc aggtcaaact gaccgtccag gataacggct gtggcgtgcc
    17641 tgaaaatgcc atccgcagca atcactacgg catgataata atgcgcgacc gtgcgcaaag
    17701 tttacgaggc gattgccgcg tccgccgtcg tgaatcaggt ggcaccgaag tggtggtcac
    17761 ctttattccc gaaaaaactt tcacagacgt ccaaggagat acccatgagt aatcaggaac
    17821 cggctactat cctgctgatt gacgatcacc cgatgctgcg aactggcgta aaacagctta
    17881 tcagtatggc accagatatc accgtggttg gcgaagcgag taatggcgaa cagggtattg
    17941 aactggcgga atctcttgat cccgatctga tcctgttaga tctcaatatg cccggcatga
    18001 acggtctgga aacgctggat aaactgcgcg aaaagtccct ctcagggcgc attgtggtat
    18061 tcagcgtctc taaccatgaa gaagatgtgg tcaccgcact gaaacgcggc gcggatggct
    18121 atctgttaaa agatatggaa ccggaagatc tgctgaaagc attgcatcag gcagctgctg
    18181 gcgaaatggt attaagcgaa gcattaacgc ctgttctggc cgccagcttg cgcgctaacc
    18241 gtgccactac tgagcgcgat gttaaccagt taaccccacg cgagcgcgat attctcaagc
    18301 tgattgccca gggtttgccg aacaagatga ttgcccgccg cctggatatc accgaaagca
    18361 cagtaaaagt gcacgtcaag cacatgctga agaaaatgaa gctcaagtct cgcgtggaag
    18421 cagcggtatg ggtgcatcag gagcgcattt tctgattacg gttcccagcg cagttcgtcg
    18481 tttgacaatg catcgaacgg ttcgacaagc gttagcgtga tctcattgga ggagacacgc
    18541 tgcccctggt tatcttccac caccacagac aatcgccagt gattgcttgc cccttccccg
    18601 ttctgccagt caggcatgat cagcgtccag ccctccgcgc tgttggcttg tgcgcccggc
    18661 gtcaaactta atatctgcgt atcgccctgc caaatcagtt gccgaatacc gtaacgactg
    18721 cggatttgta atttcagcgg cactgtttcg ccaggtttta gatcccacgg cggtgtcgcc
    18781 agaaacaccg ttaacgtttt tcgctgtcgg tactcaagag tcggtagatt atttcgctgc
    18841 ggattgtcat agcgactacc acgtaacgac tgactttcgg caacctcgcc cgcagaaagt
    18901 tgttttttga gcggtacacc aaagcggtaa ttaagattca gcccgaggtt attttgattt
    18961 tcgccacttt ccccctgttt atgctgggcc gtcacagtga ctaatggcac aggggtgtaa
    19021 tttaatccca gactcaacgc gacgggattg tgataacccg taccagagtt aaacaaatca
    19081 acacgatcac caaaatactg ttctaggctg acactggtat tgaggtgttg atagaacggc
    19141 atgcgcatcc gagctgtcag gtcgtacccg cgcgccatcc gttgttcctg cgtggctgtc
    19201 tgttcatgcc atgcagcaaa cggctgataa aagtttgccg ataatcgcaa atattcgccc
    19261 cacgcttcgg caccaaagcc cgctcgctga agattttcgt cctgcaagtt gtcataaaaa
    19321 gtgttataac ccaccagcca gttgccgcgc gcccagcgtt gcccaacgcc cacattgctc
    19381 accaacccat tatcctgctg agtaagacca agctggctcc aggtgagata acgatcatta
    19441 tcttgtaacg gcacaaacca gcttccacga ctgccggtga aatgtccttc gttatccact
    19501 ttgacgtcaa cactggcatt tccccacggt gatagccagg actctacgtg ctgattaacc
    19561 tgttgactaa gcgcgtcgcg gacttttccc aatgcgaaag cttttgcctg ctcgccagta
    19621 tccagcccgt tatcattcat actggtttcg ccgaaatctt tcacaatttc agcaaagtgt
    19681 ttttccccat catgattttc gggagccatg cctaaatccg gcagaccatc attgttatta
    19741 tcaaagggat ttgccgcttt ttgctcgaag gtagattgtg cgttagctgt accgcctgct
    19801 acaagcaaga gtaaataaaa cgggataatg cgaggaacga aacggctcaa aacgtcggta
    19861 acttccggta ataatgaaaa accatagcct taaagaataa cgattactgg caacttttgt
    19921 cgtattttct cgcgctttca ggaaaatcct taaaaatgtg accgattggt cagacgctgc
    19981 agaagcagta agatagaaga tgagtgtcta attccctgaa tacaggagta aataatgcaa
    20041 aaaatagtga tcgttgccaa tggcgcacct tacgggagcg aatccttgtt taacagcttg
    20101 cggctggcca ttgcgttacg agagcaggag agcaatctgg atctgcgtct gttcctgatg
    20161 tctgatgcgg tcacagccgg gttgcgcggg caaaaaccag gggaaggcta caacattcag
    20221 caaatgctgg agatccttac cgctcagaat gtaccggtga aattgtgcaa aacctgtacc
    20281 gacgggcgcg ggattagcac acttcctctg attgatgggg tggaaatcgg tactcttgtg
    20341 gaactggcgc aatggacgct gtcagccgat aaagtgctca cattttaatg tttaaggggg
    20401 cgtctgacgc cccactatga gggaagttta ctcaggcgaa tcccggcctt aacacaccat
    20461 ccggataatt ctccgccagt agttttttta ccgataccag caaatcattc agcccatcgt
    20521 cctgcattcc cagtttgata agctcctgtt ccagtgaaaa caggtattgt gcattggttc
    20581 ccagcggacc gctcgccgcc gcaatcaacg gcgcgatgac ctgagcgcga gtgtcagatt
    20641 catattctgg atgtcgcggg tccataataa acacaatggc gtttactgtg cgtccatcat
    20701 caagatcaag ctgacaccag gttggcagat aacagccggt aatcatctcg cgcttccaca
    20761 acagggttag ttcctgctcc agcgtctctt ctggtagtcg ataggcgacg cctgtggtgc
    20821 gtccgccctc tttcagtgca agcatccgtc ccggctggtg cgcagtcccg cgcccggcgg
    20881 tcaggcgcag gcaaaacgcg cgatgccatc caaccagtgt accggtgcat gattcggtaa
    20941 actccagtgc cggattccac atcaacgatc cataaccgaa gatccacacc ggtccctcat
    21001 caggtcgaca agccagcgtc gccgccagcg aagccgcccg ttgttctgct gaccataaga
    21061 gtgattcttc aatggcacca aatgccgttt tacaatcggc attcatcaag aaatcacgcg
    21121 ttatcacctt acacctccgc cactgcatac ttccctgcga caactttttg gtgtcttccg
    21181 gacattttta ccgctcattt tgaaaccact aacggaacaa tatgcaatcc acggaccact
    21241 tgcaaggcaa caccgcacga atagctttaa ctaagctacc ggttttacga ttttttatgc
    21301 catttatcat catccccttt ggcatattca tgcttcacag cagcccaggc cactttatgc
    21361 gcggtttctt cgcgactggc gtcatcacgc cgatcttctt tatctttata ttgatcccat
    21421 gcgctgttga acgcttcttt atagatatcc tgggcatgag acggtagaac gtgctttacg
    21481 ctttccggca gatcgctttt cgttttatac ggcatcgttg actcctttta caaagttgaa
    21541 cattaagtgt ggtcaacaat atctactctt gccaaaacat aagaattttc atgtcagact
    21601 gttcttaaga aacgctaaaa caaacattgt ctaaacgtaa tacttatttt tatgccccaa
    21661 tgagtaaaaa atcgacagcg gggtaaaaat tcgtaaaatg ttaccgatat ccagacactt
    21721 tctgttattt ttcctaccta ttttttacat tttcgataaa ttcatctgca aagggataac
    21781 cataatgtca aatgctcaag aggcggtaaa aacccgccac aaggagactt cgcttatttt
    21841 cccggttctg gtgctggtag tgctgttcct gtggggaagt agccagacac taccagtggt
    21901 cattgccatc aatcttcttg cgcttattgg tattttaagt agcgccttta gtgttgtccg
    21961 tcatgcggac gtattagccc atcgcctggg agaaccttac ggttcgctta ttcttagcct
    22021 ttcagtggtt attcttgaag tcagtttgat ttcagcttta atggcaaccg gcgacgccgc
    22081 gccaacgcta atgcgtgata cgctctattc aatcattatg attgttaccg gtgggctggt
    22141 tggcttttca ttattgttgg gcggtcgtaa gtttgccacc caatatatga atctgtttgg
    22201 tatcaagcag tatttaattg cgctgttccc cctggcgata atcgtactgg tatttccaat
    22261 ggctctgcct gcggcgaatt tttcaaccgg tcaggcgtta ctggtagcat taatttctgc
    22321 ggcaatgtat ggcgtatttt tgctgatcca ggccaaaacg catcaaagtt tgtttgtcta
    22381 cgagcacgaa gatgacagtg atgacgacga cccgcaccac ggtaaaacgt ctgcccatag
    22441 cagcctgtgg catgctatct ggttgattat ccatctgatt gccgttattg cagtcaccaa
    22501 aatgaacgcc agctcgctgg agacattgct cgacagcatg aatgcccctg ttgcctttac
    22561 tggcttcctg gtggcactgt tgattctgtc gccggaaggt ttaggtgcgt taaaagcagt
    22621 gttgaacaac caggttcagc gcgcgatgaa tctgttcttt ggttcagtgt tagcaacaat
    22681 ttcgcttacc gtacctgtcg tcacgctaat tgcctttatg acgggtaacg aattgcagtt
    22741 tgcacttggt gcgccagaaa tggtggtgat ggtggcctct ttagtgctgt gccatatctc
    22801 cttctccacc ggacgtacta acgtgctcaa tggcgcagcg catctggcac tgtttgccgc
    22861 ctatttgatg acgatatttg cctgacagtt atatctcctc cgatgacgga aatcgggaga
    22921 gataagataa attcctgctt attgtatttc ttcatactac ctggacagct cgttaatcag
    22981 aattgcgagc ctgtaccaca acctgcacga aaccactgca acagctgttg aaaagccgga
    23041 atgtactctt tattgcaaac atttcaggga tgaagtgaag atgaattgag acgtagtatc
    23101 cacaccaagc cggaattatc cggtgaggcg caatgttgcg ggggctttat ccctggtggc
    23161 attggttgct ggaaagagaa aacccccgca cgttgagagg taaaacctga caacaaaacg
    23221 ggggctaatc ttgaaaccag acacttcaag aatagccgca aaatgttgtc attacaacaa
    23281 ggcggctata tgacgctcgc gcagtttgcc atgactttct ggcacgacct ggcagcaccg
    23341 atcctggcgg ggattattgc cgcagcgatt gtcagctggt ggcgtaggtg aaaacgggcg
    23401 tcaggctgcc gtgatggcaa tatgcgcccg aaaccggccg caggggtaac agcctgcggc
    23461 cttttttgta tctggcaccc atgaaaaaag tcttaacgca gaacgctaag actttatttt
    23521 tcaagcaaaa aagattactt gctggtatcc agttcttcga aacctttcac cagatcatca
    23581 atcgctttca tctgcttgag gaacggttcc agtttagcca gcggcagcgc ggatggacca
    23641 tcacatttcg catgttccgg atccggatgc gcttcaataa acagccccgc cagacctacc
    23701 gccataccgg ctcgtgccag ctcagccacc tgagcacgac gaccaccgga agctgcgcca
    23761 aacggatcgc ggcattgcag tgcgtgggtc acgtcgaaaa tcaccggcga gttaccagac
    23821 actttcttca taatgctgaa gcccagcata tcgacaacca ggttgtcata gccgaagtta
    23881 gcaccacgat cgcaaagaat cactttttcg ttgccgcctt ctttgaattt atcaacgata
    23941 ttacccatct gtcccgggct gacaaactgt ggtttcttga cgttaattac cgcaccggtt
    24001 ttcgccatgg cttcaaccag gtcagtctgg cgagcaagaa acgccggcaa ctgaatcaca
    24061 tccacgacat cagcaacggg ctgtgcctga cttggttcgt gaacgtcggt gataattttc
    24121 acgccaaaag tctgcttcaa ctcctggaag attttcatcc cttcttccag gcccggtcca
    24181 cgataagagt ggatggagga gcggttggct ttgtcaaaag aggctttgaa cacgtaaggg
    24241 atacccagtt tctgggtcac agttacgtag tgctcgcaaa tgcgcatcgc cagatcgcga
    24301 gattccagca cgttcatacc gccaaacagt acgaacggca ggtcatttgc tacgttgatg
    24361 tcgccaatgc taaccacttt ttgtttcata ggatcgcctt aatcttgagt aaaatgtcga
    24421 ttaattaatg cagcacaata tgtttatgcg cgatgttatt tatttgcgca cggatcattt
    24481 cgctgatcgg gtcttccgga caccgttcaa cgaaataact taaatcgttc aacgcaacgt
    24541 gttcgcaatc cagttgcgca taaatcaacc cacgatcgcg aatttcatag ggatcttcag
    24601 ggttgaattg taataaagct tcgctggtgc gtaacgccag ctccatctga ttttcttcca
    24661 tcaacgaggc tttgagtgta tccagcaatt tgcggattac ctcaatgtta tcagcttcat
    24721 caaggtcttc ataaaacagt tccgccgacg ggctgatatt tccctttaac catccgtcca
    24781 gcatatgttc gcttaacgat tcaccgttaa aaggattaat cagccaaatt tcgccatccg
    24841 gacattcaat gcgcaatatc agctgcgtag ggaaaatcac cggcagcagc ggcaaatcga
    24901 gacgattcgc gacccataat aaaaccgcac ctaatgatac cgcactgccc tgtcgattct
    24961 ttaacacctg gtccagccat aatgcatcgg aaagacgata aacgccgcgt gaggctttaa
    25021 atccccagtc gccgtaaaac agcgcgatca gtttttccag ctgctcttct aaaggcagaa
    25081 gctggctgat ttcttccttc gccagactaa cgagacgctc cagttcgtcg taaacatctt
    25141 gcgagggaaa atcgcggcgg attgcttcgc aagccaggat catgccttcg cacaatggcg
    25201 ctttattaaa ttcgaaatca gctaacgatc tcatgactta ccccagtaac ggtacttttg
    25261 tggtggcgag tttaatgatg atgtacagca ccaccaacgc cagcgggaag gcaataatgc
    25321 gcgcctgctg actgtgcata cgacgataat cgagtgcaat aaaacccaaa acgatataaa
    25381 tgataactcc aaacagcttt tcagtcagcc actgtgcctg cccggaaaat ggcaggatgt
    25441 gcgctttagc catcaacgca atgccgctta acaataacac cgtatcgaca accggcggca
    25501 caatgcgcgt ccagcgagca aatgcctgtg gatgcttctg ataacgtagc cagaaacgta
    25561 aggttaatag cccaacagaa agcgcgatac taataagatg aacactaagc agtgtagaaa
    25621 aacttgtcat tgataatagc ggccgagcgt tacgcgctcg ttatcaccat agtcacggca
    25681 ggtttcgacg tcatgatacc ccgcgaggat aaatgcttgt cgcaccgctt cgccctgctg
    25741 ccagccatgt tccagaagca gaaagccgcc ggataccagc gcgttacgcg actgttcgat
    25801 gatatgcacg atgtctgcca ttccactgtc tgccgcaacc agcgcagtga gcggctcaaa
    25861 gcggacatcg ccttgttgaa gatgtgggtc ctgctcgtca atatacggcg gattgctgac
    25921 aatcatcgca aactgctgcc cggctagcgc gctaaaccag tcgctttgca gaatgtggat
    25981 atttttgatc gccagatgct gggcattacg ttgtgccagg gagacagcat caggcatacg
    26041 atctacagcg ataatttcgc agtccgggcg ctcgctagcc agcgcaagcg caatcgcccc
    26101 ggtacccgtc ccgagatcga gaatacggca aggttgttca ggcaaccgcg ccagtgcctg
    26161 ctccaccaga cactccgtat ccgggcgcgg aattaaggtc gctggcgaaa caaataacgg
    26221 caacgaccag aattctcgca ccccggttaa atgagcaatg ggttcaccat cgcgacgacg
    26281 tgtcagtagc gcatcaagtt gctgacattg ttcgtcagtc agctgcgttt caccaaaggc
    26341 gagaataaaa gtacgccctc tgccggtaac atgctccagc aggatttcag catcacgccg
    26401 cgggctttcg ctcgcctgaa gttggcttat tgcttcacgt aaccagtgtt gatattccat
    26461 tattcctgct cggacaacgc cgccagttgg tcggcctgat gttcctggat aatcggttca
    26521 atcagcatat ccagcttacc ttccatcact tcatccaggc ggtagagcgt caggttgatg
    26581 cggtgatcgg taacgcgccc ctgcgggaaa ttgtaagtac ggttacggtc gctgcgatcg
    26641 ccactcccca gcaggttacg acgggtagac gcttcggcct gttggcgttt tgccatttca
    26701 gcagcgtgga tgcgagcacc gagcactgaa agcgccttcg ctttgttttt atgttgtgaa
    26761 cgttcgtcct gacattcaac aacaatcccg gtcggcaagt gagtaatacg aattgccgaa
    26821 tcggtggtgt taacgtgctg accacccgcc cctgacgagc ggaaagtatc aatgcgtaaa
    26881 tctgctgggt tgatgtccgg cagttctgcg tcaggcagtt ctggcattac cgcaacggta
    26941 caagcagaag tatgaatacg accctgcgat tccgtagcag gaacacgttg cacgcgatga
    27001 ccgccggatt caaatttcag acgaccatac acaccatcac cgctaatttt ggcgatgatc
    27061 tctttataac caccatgttc accctcgctg gcgctcatga tttctacccg ccagcggcgg
    27121 gcttcggcgt aacggctgta catacggaac agatcgcctg caaacagcgc tgcttcgtcg
    27181 ccgccggttc cggcacggac ttcgaggaag gcgttacgtt cgtcatcagg atcttttggt
    27241 agtaatagaa cctgtaattg ctgttccagt tgctcgcttt tttctttagc ttcgcgcagt
    27301 tcatcctgcg ccatctcacg catttcagga tcgtcgagca tcatctgtgc ggtttcgata
    27361 tcttcctgaa cctgttgcca gtcggtaaaa cagcgcgaaa catcacttaa ctgcgcatat
    27421 tcgcgtgata atgcgcgaaa acgttcctgg tcggcgatag tttgcgcgtc acccagcaac
    27481 gcctgaactt cttcatggcg ttcatgcagg gcttccagtt tggcaacgat agaaggcttc
    27541 ataggcgtaa atgcaccctg taaaaaaaga aaatgatgta ctgctactcc agcccgaggc
    27601 tgtcgcgcag aatattcagg cgttcgttat ccccgtcacg ggcggcctgt tgaagtgatt
    27661 tcgttggcgc atggatcaag cggttagtca gtttccatgc cagatcctgc ataatggctt
    27721 gcgcgtcgcc gccctgctca agggccgcta acgctttggc ggttaactca tcgcgaacgt
    27781 gctctgcctg gctgcgatac tcgcgaatgg tttcgctggc gctttgtgct cgcagccacg
    27841 ccataaattc gctggtttcc tgagcgacaa tagtttccgc ctcaaccgct gcggctttac
    27901 gctgcgccag gttgtgcgaa atgatgcttt gcagatcgtc cacactataa agataagcat
    27961 tcgccagttt gccaacttcc ggctcaacat cgcgcggaac ggcaatatcc accaacagca
    28021 ttggttggtt acgacgactt tttaatgcgc gctccaccat gcctttcccg ataatcggta
    28081 acgggctggc ggtggaactg atgatgatat cggcttcgcg cagacgttcg tcgatctcac
    28141 tcagggcaat cacttccgcg ccaacttcat ctgccagaat ttgggcacgt tcgcgagtgc
    28201 ggttggcgat aatcatcttc tgtactttat gttcgcgcag atgacgcgct accagctcga
    28261 tggtttcgcc cgcgcctacc agcaacactg tgaccgtaga gagcgattca aagatctgcc
    28321 gcgccagcgt acaagccgca aaagcgacag acacagcact ggcaccgata tctgtttcag
    28381 tgcgaacgcg tttcgctaca gagaaagatt tctggaacat gcgttccagt tcgctggcct
    28441 tcatatgacc tttttgcgaa tcgacaaacg cttttttaac ctgaccgagg atctgcggct
    28501 ccccaagaac cagtgaatcc aggccgctgg caacacgcat taaatggcta accgcgtcgt
    28561 tatcctgatg ccagtagagg cttttacgca gatcttcttc attaagattg tgataatcgc
    28621 aaagccagcg gattaacgcc tcttgcaggt tatcctgctc ttcaacgcta agataaagtt
    28681 ccgtgcggtt gcacgtcgac agcaccacgc cgccctgcac catcggctgc gcaagcaggc
    28741 tgtcaagcgc ctgatcgagc ttatccggcg aaaacgatac acgttctcgc agcgatacag
    28801 gtgccgtttt atggttgata ccgagtgcta aaagggtcat gtctgcggga aataatacca
    28861 acgttgatag ggttagtctg cttgcatcat acaggatgcg taagatcaat aaaagagagc
    28921 gccccctttt ggagtaattg ccgaaagccg ttagattctg gcaattaaga caacttgaac
    28981 atagacgata gcggacggta acgctagcat taagggttat aactgcaacg tatctcaagg
    29041 acttgtcatc actatgcccc tgcccgattt tcgtcttatc cgcctgctac cgctggctgc
    29101 tcttgtgctc actgcctgtt ccgttaccac gcccaaaggt cctggcaaaa gcccggattc
    29161 gccacaatgg cgtcagcatc agcaagacgt gcgcaatctt aatcagtatc agactcgcgg
    29221 cgcgttcgct tatatttctg accaacaaaa agtgtacgcc cgctttttct ggcagcaaac
    29281 cggccaggat cgctaccgtc tgctgctcac taacccattg ggcagcacgg aactggagct
    29341 gaatgctcaa ccgggtaacg tgcagttagt cgacaataaa ggtcagcgtt ataccgccga
    29401 tgacgccgaa gagatgattg gcaaattgac cggaatgcca attccgctca acagcttgcg
    29461 ccagtggatt ttaggtttac cgggtgatgc aaccgactac aaactggacg accagtaccg
    29521 cctgagcgaa attacctaca gccagaatgg caaaaactgg aaggttgttt atggtggtta
    29581 tgacaccaaa acgcaacctg cgatgccagc caatatggaa ctcaccgacg gtggtcaacg
    29641 catcaagtta aaaatggata actggatagt gaaataatgc ggacacagtg gccctctccg
    29701 gcaaaactta atctgttttt atacattacc ggtcagcgtg cggatggtta ccacacgctg
    29761 caaacgctgt ttcagtttct tgattacggc gacaccatca gcattgagct tcgtgacgat
    29821 ggggatattc gtctgttaac gcccgttgaa ggcgtggaac atgaagataa cctgatcgtt
    29881 cgcgcagcgc ggttgttgat gaaaactgcg gcagacagcg ggcgtcttcc gacgggaagc
    29941 ggtgcgaata tcagcattga caagcgtttg ccgatgggcg gcggtctcgg cggtggttca
    30001 tccaatgccg cgacggtcct ggtggcatta aatcatctct ggcaatgcgg gctaagcatg
    30061 gatgagctgg cggaaatggg gctgacgctg ggcgcagatg ttcctgtctt tgttcggggg
    30121 catgccgcgt ttgccgaagg cgttggtgaa atactaacgc cggtggatcc gccagagaag
    30181 tggtatctgg tggcgcaccc tggtgtaagt attccgactc cggtgatttt taaagatcct
    30241 gaactcccgc gcaatacgcc aaaaaggtca atagaaacgt tgctaaaatg tgaattcagc
    30301 aatgattgcg aggttatcgc aagaaaacgt tttcgcgagg ttgatgcggt gctttcctgg
    30361 ctgttagaat acgccccgtc acgcctgact gggacagggg cctgtgtctt tgctgaattt
    30421 gatacagagt ctgaagcccg ccaggtgcta gagcaagccc cggaatggct caatggcttt
    30481 gtggcgaaag gcgttaatct ttccccattg cacagagcca tgctttaagc cgggcaagct
    30541 gagtttcggt gacaacgtca ccttgttcca gacgttgcat cgcgctcttt aatacaccgc
    30601 ctggaaagga tcatgcctgg cccgcacagt tttcggcaga ttctttccac caatggacgc
    30661 atgcctgagg ttcttctcgt gcctgatatg aagctttttg ctggtaacgc caccccggta
    30721 ctagcacaac gtattgccaa ccgcctgtac acttcactcg gcgacgccgc tgtaggtcgc
    30781 tttagcgacg gcgaagtcag cgtacaaatt aacgaaaatg tacgcggtgg tgatattttc
    30841 atcatccagt ccacttgtgc ccctactaac gacaacctga tggaattagt cgttatggtt
    30901 gatgccctgc gtcgtgcttc cgcaggtcgt atcaccgctg ttatccccta ctttggctat
    30961 gcgcgccagg accgtcgcgt ccgttccgct cgtgtaccaa tcactgcgaa agtggttgca
    31021 gacttcctct ccagcgtcgg tgttgaccgt gtgctgacag tggatctgca cgctgaacag
    31081 attcagggtt tcttcgacgt tccggttgat aacgtatttg gtagcccgat cctgctggaa
    31141 gacatgctgc agctgaatct ggataaccca attgtggttt ctccggacat cggcggcgtt
    31201 gtgcgtgccc gcgctatcgc taagctgctg aacgataccg atatggcaat catcgacaaa
    31261 cgtcgtccgc gtgcgaacgt ttcccaggtg atgcatatca tcggtgacgt tgcaggtcgt
    31321 gactgcgtac tggtcgatga tatgatcgac actggcggta cgctgtgtaa agctgctgaa
    31381 gcgctgaaag aacgtggtgc taaacgtgta tttgcgtacg cgactcaccc gatcttctct
    31441 ggcaacgcgg cgaacaacct gcgtaactct gtaattgatg aagtcgttgt ctgcgatacc
    31501 attccgctga gcgatgaaat caaatcactg ccgaacgtgc gtactctgac cctgtcaggt
    31561 atgctggccg aagcgattcg tcgtatcagc aacgaagaat cgatctctgc catgttcgaa
    31621 cactaatcga acccggctca aagacccgct gcggcgggtt tttttgtctg taatatccat
    31681 ttgtatgacc tatgcctcct tcacctgcca tttagttgac agatgatgcg ctcacggatg
    31741 aaacattatt gtgaacaaaa tattttcctc acatgtgatg cctttccgcg ctctgatcga
    31801 cgtttgctgg aaagaaaaat atactgccgc acggtttacc cgtgacctga ttgccgggat
    31861 aaccgtcggg attattgcta tcccgctggc gatggcgttg gctattggta gtggtgtggc
    31921 cccccagtac ggtttatata ccgcagctgt tgcggggatt gtcattgctc taacgggtgg
    31981 gtcacgcttt agcgtttccg gtccgactgc ggcatttgtg gtaattctct atcccgtttc
    32041 gcaacagttt ggactggcag gactgctggt tgcgaccttg ttgtcgggaa tctttttgat
    32101 tctgatgggg ctggcacgct ttggtcgtct gattgagtat attccggttt ccgtcacctt
    32161 aggtttcacc tcgggtatcg ggatcaccat cggtaccatg cagattaaag attttctcgg
    32221 tctgcaaatg gcccatgtcc cggaacatta tctacaaaaa gtcggcgcat tatttatggc
    32281 gctgccgacc attaatgtgg gtgatgctgc cattggcatt gtgacgcttg gcattctggt
    32341 tttctggccg cgtctgggca ttcgtttacc cggtcacctt ccggccttgc tggctggttg
    32401 cgcggtgatg gggattgtta acctgctcgg cggacatgtt gctaccatcg gttcgcaatt
    32461 ccactacgtc ctggccgatg gttctcaggg taacggtatt ccgcaactgc tgccgcaact
    32521 ggtgctgccg tgggatctgc ctaattcaga attcacgcta acctgggatt ctattcgcac
    32581 cctgctgcct gcggcattct caatggcaat gctcggggcg atcgaatctc tgctctgcgc
    32641 tgtggtactg gatggtatga ccgggactaa gcacaaggcg aatagtgaac tggtcggtca
    32701 ggggctgggg aatatcatcg ctccattctt tggtggtatt accgctactg ctgccatcgc
    32761 gcgttctgcc gctaacgtcc gtgccggggc aacttcccct atctcggcgg tgatccactc
    32821 tattctggtt attctcgccc tgctggtact ggcaccactg ctctcctggc tgccgctttc
    32881 cgccatggca gccctgctgt tgatggtggc gtggaacatg agtgaagcgc acaaagtggt
    32941 cgacttgctg cgtcatgcgc cgaaagatga catcatcgtc atgctgctgt gtatgtcact
    33001 gaccgtgctg tttgatatgg ttattgccat cagcgtgggg atcgtgctgg catcgctgct
    33061 gtttatgcgt cgtatcgcac gtatgactcg cctggcaccg gtagtcgtag atgttccaga
    33121 cgatgtcctg gttctgcgcg ttattggccc gctgtttttt gctgctgctg aaggcttatt
    33181 cacggacctg gagtcacgtc ttgaaggcaa acggattgtg attctgaagt gggatgccgt
    33241 tccggtactt gatgctggtg gtcttgatgc gttccagcgt tttgtgaagc gtctgcccga
    33301 aggatgtgaa ctgcgcgtgt gcaacgtgga attccagcca ctgcgaacta tggctcgcgc
    33361 aggcattcaa ccgatcccgg gacgcctcgc gttcttcccg aatcgtcgcg cggcgatggc
    33421 ggatttataa gtacgagatt gaccagtcag cgcaaactga ctggtcagca aactgcatta
    33481 tttgttagct atgacggcga ttatcgctac ggcggcaacg tttgtcatag tgacgaaccc
    33541 accagtaacg gtcgcacacc tgttcatgcc cgccaacacg ggcacccgcc agccagagaa
    33601 tggcaccgac gaaaatactt agcactgcac catgtgcgaa atactggggc atattaaact
    33661 gtggtaactg gttgaggatt gaatacccca cgccgaccac cattaccacc agacccaacc
    33721 ccatgagcac gttaccgagt atcgaagcgt ttttgcgttt catatgtcac ctccggaact
    33781 ttctgggttg taacagggaa tacccctctt ccttatgtgt aaagtataga caacaccctg
    33841 tgggttatgt gcgggcgtaa tcacaattac aacccttatt tcaacaaaac tttacaaata
    33901 aacgcctgaa caccttgact ttctaatagt ccactcaggc aattattacg ttatctgcat
    33961 ttgatacgca gttttttgtc aagcggggcc gcaaccagta aactacgcgc cagttatgta
    34021 cacactcagg acaaaaaaac gtgacgatta aattgattgt cggcctggcg aaccccggtg
    34081 ctgaatacgc cgcaacgcga cataatgctg gtgcctggtt cgttgactta ctggcagagc
    34141 gtttgcgcgc tccgctgcgc gaagaggcta aattctttgg ttatacttcg cgagtcaccc
    34201 ttggaagcga agatgtccgc ctgttagtcc cgactacatt tatgaatctc agcggcaaag
    34261 ccgttgcggc gatggccagt tttttccgca ttaatccgga cgaaattctg gtggcccacg
    34321 acgaactgga tctgcctcct ggcgtcgcca aatttaagtt gggcggtggc catggtggtc
    34381 acaatggact gaaagacatc atcagtaaat tgggtaataa ccctaacttt caccgtttac
    34441 gcatcggaat cggtcatccg ggcgataaaa ataaagttgt cggttttgtg ttaggcaaac
    34501 cgcctgttag tgaacagaag ttaattgatg aagccatcga cgaagcggcg cgttgtactg
    34561 aaatgtggtt tacagatggc ttgaccaaag caacgaaccg attgcacgcc tttaaagcgc
    34621 aataagtcgt tgtctgcggc atttttgccg agtgccgtgt ataataggca aagttatttc
    34681 catttctgca atctgttagc aataacaggt tgattattaa gatattaagg tgatttaaat
    34741 catgggattc aaatgcggta tcgtcggttt gcccaacgtc gggaaatcta ccctgttcaa
    34801 cgcgctgacc aaagccggta ttgaagcggc caactttcca ttctgcacca ttgagccgaa
    34861 cacaggcgtc gtaccaatgc ctgaccctcg cctggatcaa ctggctgaaa tcgtaaaacc
    34921 gcaacgtacg cttcccacga ccatggaatt tgtcgatatc gccggtctgg taaaaggcgc
    34981 atcgaaaggc gaaggtctgg gtaaccagtt cctgaccaac atccgtgaaa ccgaagcgat
    35041 cggtcacgtt gttcgctgct ttgaaaatga caacatcatt cacgtttccg gcaaagttaa
    35101 cccggctgac gatattgaag ttatcaacac cgaactggcg ctggcagacc tcgacacctg
    35161 cgaacgtgcg attcatcgcg tacagaagaa agccaaaggt ggcgataaag acgcgaaagc
    35221 tgagctggcg gtcctggaaa aatgcctgcc ccagctggaa aacgcaggta tgctgcgcgc
    35281 gctggattta agcgctgaag agaaagcggc tattcgttac ctgagcttcc tgacgctgaa
    35341 accaacaatg tacatagcca acgtcaacga agatggtttt gaaaacaacc catatcttga
    35401 ccaggtgcgt gaaatcgcgg cgaaagaagg ttctgttgtg gttccggttt gtgctgctgt
    35461 tgaagcagac attgccgaac tggacgacga agaacgtgac gagtttatgc aggagcttgg
    35521 gctggaagaa ccgggcctga accgtgtgat ccgtgccggt tataagctgc tgaacctgca
    35581 aacttacttc accgctgggg tgaaagaagt gcgtgcatgg accattccgg ttggagcaac
    35641 cgcgccgcag gcagcgggca aaatccatac tgattttgaa aaaggcttta tccgtgcaca
    35701 aaccatctcg tttgaagatt tcatcactta caaaggtgaa caaggcgcga aagaagcagg
    35761 caaaatgcgt gcagaaggta aagattacat cgtgaaagat ggcgatgtga tgaacttcct
    35821 tttcaacgtc taattaattt attgtctcat gaggtttcgc taaatctcat gaagagcgca
    35881 aaagtatata aaatccacgc aattgcgtgg atttttcatt tcaccgagtc ctaatcattc
    35941 ttgtaacatc ctacactttt gtgcttattg cctacagtga accacgagaa taattaaaga
    36001 cataagtgtt cagatgactg atatctcatt agcagtgata taacgtcagt ttttgttcca
    36061 ctacacatca gccctcttca cagctgatca atattgatct cgctccaata tgctggcgag
    36121 aaaaggactt acctgaaggg taatatcccg cagataaagt ccccgaataa cgtgattacg
    36181 tgcgatcctg gcaagatgaa aatgataacc agtagcacaa taataacgat aggccagaaa
    36241 atccattgaa caaaaatgaa tatcgatgtt ttttgctttt tcttattagt aatgcaaaaa
    36301 tggcgcatat tgtttttgtt atgcagatac atcaagcgaa aatcccataa ctaattagga
    36361 ttattacaaa aaatcttcac caaatttttt aaccgctgtt tgttgtaata tttttgccat
    36421 attcatagta acgaattgga ataagtaacg acaacttagg gaataactcg atataaactc
    36481 acctttgtaa ggggggggtt aaatatatat taattcctgt gttcctgatt ttgcccggta
    36541 atagagaatt attctcagag aagctcatca tgattgcacc atacagatat catgatgata
    36601 agaggatatt attcattgac ttaaacaaaa tgacaaaatc catagaccca tagcctgggt
    36661 actatatagg agtggtatgg gcatcaaaca acacaatggg aataccaaag ccgatcgtct
    36721 cgctgaatta aaaatccgtt cgccctcaat tcaactgata aaatttggcg ctattggttt
    36781 gaatgcaatt atcttttccc ccctgctgat agctgctgat acaggaagtc aatatggcac
    36841 caatattact attaatgatg gtgacagaat tacaggagat accgccgatc catcaggaaa
    36901 cctctatggt gtaatgaccc cagcaggaaa cacgcctggc aatatcaacc tgggtaatga
    36961 tgtcaccgtc aatgtcaacg acgcctctgg atatgcaaaa ggaatcatta ttcagggcaa
    37021 aaacagctcc ctgacagcta accgactcac agtagatgtt gttggtcaaa cctctgccat
    37081 cggcattaac ttaattggtg actataccca tgctgactta ggcacaggca gcaccattaa
    37141 gagtaacgat gacggcatca ttattgggca tagctcaaca ctaacagcca ctcaattcac
    37201 cattgaaaac tcgaacggta taggcctaac catcaatgac tatggcacca gtgtcgatct
    37261 tggaagcgga agtaaaatca agaccgatgg aagtacaggt gtttatatcg gtggtctcaa
    37321 cggcaataac gccaatggtg ctgcgcgttt tacggcgaca gacctgacaa tcgatgttca
    37381 gggctacagc gccatgggga taaacgtaca gaaaaactct gttgtcgatc tcggaacaaa
    37441 cagtaccatt aaaaccaatg gcgataatgc tcacggcctc tggagctttg gccaggttag
    37501 cgcgaatgca ctcactgttg atgtaactgg agccgcggcc aatggcgtcg aagttcgtgg
    37561 tggtacaacc actatcggtg cagatagcca tatttcttcc gcgcagggcg gtggcctcgt
    37621 caccagtagt tcagacgcga caatcaattt ttctggcacg gcagcgcaac gaaacagcat
    37681 cttttccggc ggttcttatg gtgcctcggc ccagacggca acggctgtta tcaacatgca
    37741 aaataccgat attacggttg atcgtaatgg cagtctggcg ctgggtttgt gggcgctcag
    37801 cggcggtaga ataaccggag acagtttggc tatcaccggc gcggcaggag ccagagggat
    37861 ttatgccatg accaacagcc agatcgacct cacgagcgat ctggtcattg atatgagtac
    37921 acccgaccag atggccatcg caacgcaaca tgacgatggt tatgccgcca gccgcatcaa
    37981 cgcctcgggt cgtatgctta tcaacggtag cgttctttcc aaaggtgggc taatcaatct
    38041 ggatatgcac cctgggtcgg tttggacagg ttcctccctc agcgataatg tcaatggcgg
    38101 gaaactggac gttgcaatga ataacagcgt ctggaacgta acaagtaatt ctaatctcga
    38161 cacgctggcg ctgagccatt caactgtcga ttttgccagc cacgggtcaa ctgccggcac
    38221 atttaccaca ttaaacgtag agaacctgag cggtaacagt acctttatta tgcgtgctga
    38281 tgttgttggc gagggtaatg gcgttaataa tagaggggat ttattgaata tcagcgggag
    38341 tagtgctggt aatcacgtat tggctatccg caaccagggc agcgaggcca caacgggaaa
    38401 tgaagttctg acagtggtaa aaaccactga cggcgcggcc tcgttcagcg cgtcttctca
    38461 ggttgagttg gggggatatc tgtacgatgt gcgtaaaaat ggcactaact gggagcttta
    38521 cgcttccggg acagttccgg aaccgactcc taatcctgaa cccacaccag ctcccgctca
    38581 gcctcccata gtcaaccccg atcctacgcc tgaacccgct cccacgccta aacccaccac
    38641 gaccgcagat gctggcggca attatctcaa tgtcggttac ttattgaact atgttgaaaa
    38701 ccgtacgctg atgcaacgga tgggtgactt gcgaaatcag agtaaagacg gtaatatctg
    38761 gttgcgcagt tatgggggaa gcctggactc ctttgccagt ggcaaactga gcggctttga
    38821 catgggttac agcggtatcc agtttggtgg ggataaacgt ctctctgatg taatgccgtt
    38881 gtatgtcggt ctgtatattg actcaacaca tgcatcgccg gactatagcg gaggcgacgg
    38941 taccgcacgt tcagactaca tgggaatgta cgccagttac atggcacaaa acggttttta
    39001 cagcgatctc gttataaaag catcgcgcca gaaaaatagt ttccacgtac tggacagtca
    39061 gaacaacggc gttaacgcca acggcactgc gaatggaatg agcatctccc tggaagccgg
    39121 gcagaggttc aacctgtccc ctactggtta tgggttctat atagagccgc aaacccagct
    39181 tacatacagc caccagaatg agatggctat gaaggcgagt aatggcctca atatacatct
    39241 gaatcactac gaatcgctgc tggggcgtgc cagcatgata ctggggtatg acatcaccgc
    39301 aggcaacagc cagctgaatg tctatgtgaa gactggcgct atccgcgagt tttcagggga
    39361 taccgaatat ctgttgaacg actcccggga gaagtacagt ttcaaaggta atggctggaa
    39421 taacggcgtg ggagtcagtg cacagtataa caaacagcac acattctatc tcgaagcgga
    39481 ttacacgcag ggtaacctct ttgatcagaa gcaagtcaac ggaggatatc gcttcagctt
    39541 ttaagtgcgg gatagacaag cccttaaacg atgctgaaaa ttgttagcgt gcgccggaat
    39601 gaaatacaga tcactctgat gtatcagaaa tcgtttctgt ctttcatacc cggcgcttaa
    39661 actgccctgc atcaatgcca tgttgcttca ttttccgcca cagcgtagtg cggccgatcc
    39721 caagtaaagc cgacatttcc tgaatgcgac cgcctgtgac ctgggctgcg ttaataattg
    39781 cctctttttc aacttccgca aatgacagac tggtggaaag gcgggtggcg ctgacatcat
    39841 ctgtcgcctg ctcggtaaac agatgttcag gtaaatcact gacgcgaatg cgcccgttat
    39901 cactactcag agcaagattc tcgatgacgc tgtaaagttc aaaatcgttg cctggccatg
    39961 cacaagaaac taggcgagcg agggcatcgt catcaatttt cagccgcgta gagaagcgtt
    40021 tttcaagact gcgtaatttg ttattcacca gcgccggaat gctgccacgc cgcatacgca
    40081 gaggcgggat ggtaatttca aatgcatgca gcgcgtaata cagctggcga ctaaaacgat
    40141 tttgttccac cagcattgcg aggtccgcgg tcgttgttgc aatcactttg acatcaattg
    40201 gtattaaacg ccgcgcatcc agtcgcgtga taaccccctg cttgataacc tgcagcaaag
    40261 cagactgtaa ctccaccgcc agatattcaa tcttttcaag aaacagcgtg ccgccgtgtg
    40321 ccagttccag ccgactcaga cggccatttt cattgtccgt gcgatcgcca ccaataaatt
    40381 cttccgccag cgcagcatca ccatataact cacaattgac ggcgatataa ggacctgcag
    40441 cacgctcgct ttcattatga attgcctgac ttagcagtgc cttgcccacg ccctcttctc
    40501 cacaaagcag gacaggaaag ctactgcgcg ccgcctggcg accaaaatga atcaagcggc
    40561 gggtttgcgg atcgtcctgt ggcatatgag cgaaggtatg gctgactttt cctaattgac
    40621 tggtcattaa ctgccgcatc tgttccacag gatggagcaa caaaataaag ctggttccct
    40681 gcgtttctat tatcggttta agggttatca ccgcatcaat aaactggtgc tggctttcaa
    40741 aggttgcttc tacgtgtttg agcggatgtg ccagttttat tgcttgttgc aatacggcgg
    40801 gtaacgttaa gagttcagtg attgcccgtc cctgacttgc cgtcgcgtca aggcgcaaga
    40861 cccgcgccgc ctgggcatta ataaattgca aattaccctg ctcgtcccag ctaatcacgc
    40921 catcatccat actttctaac agggcattaa gttgatttaa atgacggtta gtttcagcga
    40981 gcaaactgtc cgtcagcagt aaattaccga cctcgcgggc gattgccaac gtcaacggca
    41041 aatcagctgc ggtagtttgc tcaaccggac acgccagcgc tattgttccc gtcaatcggc
    41101 ccttgctgtc aaacaacggc gttgcacaaa aggcccagtt ccagagtgcc tgtttgaaat
    41161 gttgatcggc catcgttttc acggcctgac cagagatagc cgctaacgat agcgcacaag
    41221 taccaataat tccctcggcg caatacgtgc cgtcattgaa tcccagtgca cttagctgct
    41281 gcaaggtttg cggatcgcca ttacggctga gaatgcaggc ggtttcatcg aggataaaca
    41341 gcgcacactc tcgcggtgcc atatattccc aggcatcttc cagcgcagcc tgcccgagcg
    41401 tcagcatcgc tttcttacgc cgataaatag aagcaaatgt cacgccctgg gcctgatgtg
    41461 gtacgttcca tgtctcccgt ttcatcagct tattgcatcg ctcccaggag gttgcaatta
    41521 agggagatat gccccgacca tcgttgttaa aagcgccact catatcccgc atcccttatg
    41581 acaaaaggtg ttccggctcg caattttaag tattaataca cgtaaaagtt tcaatatgga
    41641 acattcatta aagcatatgt ttcactatga aacataattt gggctaattt tcttttccct
    41701 tgcctgatgc acaatggatt catcggcagt cagtggtcgc cgtgtcgttg aacatcatcc
    41761 atgccctacc gtaattgctg gagcaaaata atgaaaaaat tgatcaatga tgtgcaagac
    41821 gtactggacg aacaactggc aggactggcg aaagcgcatc catcgctgac actgcatcag
    41881 gatccggtgt atgtcacccg agctgatgcc cctgttgcag gaaaagtcgc cctgctgtcg
    41941 ggtggcggca gcggacacga gccgatgcac tgtggttata tcggtcaggg gatgctttcg
    42001 ggggcctgtc cgggcgaaat tttcacctca ccgacgcccg ataaaatctt tgaatgctcc
    42061 atgcaaattg atggcggcga aggtgtactg ttgattatca aaaattacac cggcgatatt
    42121 cttaactttg aaacagcgac cgagttactg cacgatagcg gcgtaaaagt gaccactgtg
    42181 gtcattgatg acgacgttgc ggtaaaagac agtctttata ccgccgggcg acgcggcgtt
    42241 gccaacaccg tattaattga aaaactcgta ggcgcagcgg cggagcgtgg cgactcactg
    42301 gacgcctgtg cggaactggg gcgtaagttg aataatcaag gccactcaat aggtatcgct
    42361 ctcggtgcct gtaccgttcc tgccgcgggc aaaccttctt ttacccttgc ggataatgag
    42421 atggagtttg gcgtaggcat tcatggtgag ccaggtattg accgccgccc tttctcttcc
    42481 cttgatcaaa ccgtcgatga aatgttcgac accctgctgg aacatggctc ataccatcgc
    42541 actttgcgtt tctgggatta tcaacaaggc agctggcagg aagaaccaca aaccaaacaa
    42601 ccgctccagt ctggcgatcg ggtgattgca ctggttaaca atcttggcgc aactccgctt
    42661 tctgagctgt acggcgtcta taaccgcctg accacacgtt gccagcaagc gggattgact
    42721 atcgaacgta atttaattgg cgcgtactgc acctcactgg atatgaccgg tttctcaatc
    42781 accttactga aagttgatga cgaaacgctg gctctctggg acgccccggt ccacaccccg
    42841 gcccttaact ggggtaaata aggagaaagc aatgtcactg agcagaactc aaattgttaa
    42901 ctggctcact cgttgtggcg atattttcaa caccgagagc gagtatctta ccgggctgga
    42961 tcgcgagatt ggcgacgctg accacgggct aaatatgaac cgaggcttta gcaaagtggt
    43021 ggaaaaactc cctgctatcg cagataaaga tatcggtttc attctcaaaa ataccggtat
    43081 gacgctgctt tccagcgtcg gtggtgccag tggtccgctg ttcggtacct tctttatccg
    43141 cgccgcacag gcgacccagg cacggcaaag cctgacactg gaagagcttt atcagatgtt
    43201 ccgcgatggc gcggacggcg taatcagtcg cgggaaagcc gaacctggcg ataaaaccat
    43261 gtgtgatgtg tgggtgccgg tggtggaatc gttacgtcag tccagcgagc aaaatctctc
    43321 tgttccggtg gcgctcgaag ctgccagtag catcgccgaa tccgctgcac aaagtacgat
    43381 tacgatgcaa gcccgcaaag gccgcgccag ttatctcggt gaacgcagta ttggtcacca
    43441 ggatcccggc gcgacctcgg tgatgtttat gatgcaaatg ttggcgttag ccgcaaaaga
    43501 gtaaggaatt ggtgatggta aacctggtca tagtttcaca tagcagccga ctgggagaag
    43561 gtgtcggtga attagcccgt cagatgttaa tgagtgatag ttgtaaaatc gccattgccg
    43621 cgggaattga cgatccacaa aatcccattg gtaccgatgc cgtcaaagtg atggaggcca
    43681 tcgaatctgt tgctgatgcc gaccatgtgc tggtcatgat ggatatgggt agcgcattat
    43741 tgagtgctga aactgcgctg gaattgctgg ctcccgagat cgccgcaaaa gtacgtttgt
    43801 gtgctgcgcc gttggtcgaa ggtacactgg cagcaacggt cagcgcggcc tcgggggcgg
    43861 atatcgacaa agttatcttt gacgctatgc atgcgctgga agccaaacgt gaacaactgg
    43921 gtttaccgtc ctccgacact gaaatctctg acacatgtcc tccgtacgat gaagaagccc
    43981 gttctctggc ggtggtcata aaaaaccgta acggcctgca tgtacgtccg gcctcccggc
    44041 tggtttatac cttatcgaca tttaatgccg atatgttgct ggaaaaaaac ggcaaatgcg
    44101 tcacaccaga gagtattaac cagattgcgt tactacaagt tcgctataac gatacgctgc
    44161 gcctgattgc gaaagggcca gaagctgaag aggcactgat cgctttccgt cacctggctg
    44221 aagataactt tggtgaaacg gaggaagtcg ctccacctac tctgcgtccc gttctgcctg
    44281 tttcgggtaa agccttttat tatcaaccag ttttatgtac ggtacaggca aaatcaaccc
    44341 tgaccgcgga agaagaacaa gatcgattac gccaggctat tgacttcacg ttattagatc
    44401 tgatgacgtt aacagcgaaa gcagaagcca gcgggcttga cgatattgcc gcaatctttt
    44461 ctggtcacca tacactgtta ggtgatccgg aactgctggc ggcggcaagc gaactccttc
    44521 agcatgaaca ttgcacggca gaatatgcct ggcagcaagt tcttaaagaa cttagccagc
    44581 aataccagca actggatgat gaatatctac aagctcgcta tattgatgtg gacgatcttc
    44641 tgcatcgcac cctggtccac ctgacccaaa cgaaagaaga actcccgcag tttaactcgc
    44701 caactattct actggcggag aacatttatc cttccacagt actgcaactg gatccggcgg
    44761 ttgtaaaagg tatctgcctt agcgccggaa gtccggtatc ccacagcgcc ctaatcgccc
    44821 gtgaactggg gattggctgg atttgccagc agggtgagaa actgtatgcg atacaaccag
    44881 aagaaacgct aacgctggac gttaaaacgc aacgtttcaa ccgtcagggt taagccaccg
    44941 ttcggatggc atcgttctga tgtcatccgg caatttaccc agcactgatg ttttttaact
    45001 acctgttata ttttttacca atttatgatg atttttcttc gctaatcgcg aaccaaatgt
    45061 cgctggctgg ctaataaaca gacgttattt gatcactcta tttcgccgtt atttatccct
    45121 ttaattccct tttctaaaat gcctgaccgt tcgcagaatg agatttcgat catgcagcta
    45181 gtgcgatcct gaactaaggt tttctgatac ttgaataccg ttttattcgg tttcgccaaa
    45241 ggagaatgat tgatgaaatc ccccgcacct tctcgcccgc aaaaaatggc gttaattcca
    45301 gcctgtatct ttttgtgttt cgctgcgcta tcggtgcagg cagaagaaac accggtaaca
    45361 ccacagccgc ctgatatttt attagggccg ctgtttaatg atgtgcaaaa cgtcaaactt
    45421 tttccggacc aaaaaacctt tgccgatgcc gtgcctaaca gcgatccgct gatgatcctt
    45481 gctgattatc ggatgcagca aaaccagagc ggatttgatc tgcgccattt cgttaacgtc
    45541 aatttcaccc tgccgaaaga aggcgagaaa tatgttccgc cagaggggca gtcactgcgc
    45601 gaacatattg acggactttg gccggtatta acgcgttcta ccgaaaacac cgaaaaatgg
    45661 gattctctgt taccgctgcc ggaaccttat gtcgtgccgg gcggacgttt tcgcgaggta
    45721 tattactggg acaattactt caccatgtta ggacttgccg aaagtggtca ctgggataaa
    45781 atcgcggata tggtggccaa ttttgctcat gaaataaaca cttacggtca tattcccaac
    45841 ggcaaccgca gttactattt aagccgctcg caaccgccct tctttgccct gatggtagag
    45901 ttactggcgc agcatgaagg cgatgccgcg ttgaagcaat acctgccgca aatgcaaaaa
    45961 gaatatgctt actggatgga cggtgttgaa aacctgcaag ccggacaaca ggaaaaacgc
    46021 gttgtaaaac ttcaggatag tacccttctc aaccgctact gggacgatcg cgatacgcca
    46081 cgaccagagt catgggtgga agatattgcc accgccaaaa gcaatccgaa tcgacctgcc
    46141 actgaaattt accgcgacct gcgctctgcc gctgcgtctg gctgggattt cagctcgcgc
    46201 tggatggaca acccgcagca gttaaatacc ttacgcacca ccagcatcgt accggtcgat
    46261 ctgaacagcc tgatgtttaa aatggaaaaa atcctcgccc gcgccagcaa agctgcagga
    46321 gataacgcga tggcaaacca gtacgaaacg ctggcgaatg cccgtcaaaa agggatcgaa
    46381 aaatacctgt ggaacgatca acaaggctgg tatgccgatt acgacctgaa aagtcataaa
    46441 gtgcgcaatc agttaaccgc ggccgccctg ttcccgctgt acgtcaatgc ggcagcgaaa
    46501 gatcgcgcca acaaaatggc gacggcgacg aaaacacatc tgctgcaacc cggcggcctg
    46561 aacaccacgt cggtgaaaag tgggcaacaa tgggatgcgc caaatggctg ggcaccgtta
    46621 cagtgggtcg cgacagaagg attacaaaac tacgggcaaa aagaggtggc gatggacatt
    46681 agctggcact tcctgaccaa tgttcagcac acctatgacc gggagaaaaa gctggtggaa
    46741 aaatatgatg tcagcaccac cggaacgggg ggcggcggtg gcgaatatcc attacaggat
    46801 ggctttggct ggaccaatgg cgtgacgctg aaaatgctgg atttgatctg cccgaaagag
    46861 caaccgtgtg acaatgttcc ggcgacgcat ccgaccgtta agtcagcaac gacgcaaccc
    46921 tcaaccaaag aggcacaacc cacaccttaa ccagcgctta ctccgacaac gtcattcggc
    46981 ttctccggat ggcgttgtcg gcagataaca ccatatctat ggttttttct acacagacgg
    47041 tttttcaagt cctggcggga agtattgcgc aaatgtttgc catacgtggt tgccgaagat
    47101 agcgtcattt ctacgggtaa gtgtttcact ttcatcaccc ggcgatttac gccgacgttg
    47161 gctatataaa gcatcaattg ctaccgaata ttgttacggt gttcactcat caacgttttt
    47221 ttgctttctg gagttaaatt aataatcaca taatccagac actccagaga atttagcaac
    47281 caaaaaaaga attcagacca ttcctcttcg cgtaactgaa gcattgccga acgggtattt
    47341 tggcttaatc tgccagaagg attgatcaat tctaaatgtt caataagcgc agccaaaacc
    47401 gcctcactat ccgccccatt tttaacgcct gactggctac tgtctaaaat atttattttc
    47461 tgaatcacac caaaaacaac gaaacaataa ttaaaaaagg attctgttgt tgttaccggg
    47521 aaccgactta tttcgctcag aaagtcgttc aaccagctcc gcagtgataa gattcgctga
    47581 tgatacagtg tatcctcact gcgtgctgga ggtgccagta taatatgttg atcaatacag
    47641 tatttattgt gttcagtcgt ttcctgatgc gtttggttct ctgcggattg tcgctggcga
    47701 aataaatggc ttgcggtaaa tgccgttaca gagcagcgca acgcgtcaat tccttcggag
    47761 tgcccgactt ttggcgagag ttccggtttt atgcgtccag gtagcggaaa tttcacccga
    47821 atgagaaatg cattaataaa atg
//
LOCUS       DAJTYH010000018        47348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTYH010000018 DAJTYH010000000
VERSION     DAJTYH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OO609_001037"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OO609_001037"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6632541.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO609_001038"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO609_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6632542.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO609_001039"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO609_001039"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6632543.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO609_001040"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO609_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6632544.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO609_001041"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO609_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6632545.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO609_001042"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO609_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6632546.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO609_001043"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO609_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6632547.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO609_001044"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO609_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6632548.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO609_001045"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO609_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6632549.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO609_001046"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO609_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6632550.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO609_001047"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO609_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6632551.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO609_001048"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO609_001048"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6632552.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="OO609_001049"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="OO609_001049"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6632553.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="OO609_001050"
     CDS             11231..12085
                     /locus_tag="OO609_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6632554.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="OO609_001051"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="OO609_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6632555.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="OO609_001052"
     CDS             complement(13548..14687)
                     /locus_tag="OO609_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6632556.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO609_001053"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO609_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6632557.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="OO609_001054"
     CDS             15873..17234
                     /locus_tag="OO609_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6632558.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="OO609_001055"
     CDS             17231..17749
                     /locus_tag="OO609_001055"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6632559.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="OO609_001056"
     CDS             17749..18069
                     /locus_tag="OO609_001056"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6632560.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="OO609_001057"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="OO609_001057"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6632561.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQQAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20105
                     /locus_tag="OO609_001058"
     CDS             18888..20105
                     /locus_tag="OO609_001058"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6632562.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20202..20624
                     /locus_tag="OO609_001059"
     CDS             20202..20624
                     /locus_tag="OO609_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6632563.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20672..21544)
                     /locus_tag="OO609_001060"
     CDS             complement(20672..21544)
                     /locus_tag="OO609_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6632564.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLTPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21556..22650)
                     /locus_tag="OO609_001061"
     CDS             complement(21556..22650)
                     /locus_tag="OO609_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6632565.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22683..23681)
                     /locus_tag="OO609_001062"
     CDS             complement(22683..23681)
                     /locus_tag="OO609_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6632566.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23706..25217)
                     /locus_tag="OO609_001063"
     CDS             complement(23706..25217)
                     /locus_tag="OO609_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6632567.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25240..26222)
                     /locus_tag="OO609_001064"
                     /pseudo
     CDS             complement(25240..26222)
                     /locus_tag="OO609_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26319..29420)
                     /locus_tag="OO609_001065"
     CDS             complement(26319..29420)
                     /locus_tag="OO609_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6632568.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29427..29600)
                     /locus_tag="OO609_001066"
     CDS             complement(29427..29600)
                     /locus_tag="OO609_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632569.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29718..30911
                     /locus_tag="OO609_001067"
     CDS             29718..30911
                     /locus_tag="OO609_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6632570.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO609_001068"
     CDS             complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO609_001068"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6632571.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32689..33879
                     /gene="hmpA"
                     /locus_tag="OO609_001069"
     CDS             32689..33879
                     /gene="hmpA"
                     /locus_tag="OO609_001069"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6632572.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO609_001070"
     CDS             complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO609_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6632573.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO609_001071"
     CDS             complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO609_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6632574.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO609_001072"
     CDS             complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO609_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6632575.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO609_001073"
     CDS             complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO609_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6632576.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO609_001074"
                     /gene_synonym="purI"
     CDS             complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO609_001074"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6632577.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42656..44212
                     /gene="mltF"
                     /locus_tag="OO609_001075"
     CDS             42656..44212
                     /gene="mltF"
                     /locus_tag="OO609_001075"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6632578.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO609_001076"
     CDS             complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO609_001076"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6632579.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO609_001077"
     CDS             complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO609_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6632580.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45614..46462
                     /locus_tag="OO609_001078"
     CDS             45614..46462
                     /locus_tag="OO609_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6632581.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46801..47348)
                     /locus_tag="OO609_001079"
                     /pseudo
     CDS             complement(46801..47348)
                     /locus_tag="OO609_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11288 a        12969 c        11908 g        11183 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtatattaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aacaggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagcgc agcgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca
    19441 tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt acagcgccat cagcaggctg
    19501 gtgtgcgcat tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac
    19561 tgacgctgca aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca
    19621 gcgccaacga gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg
    19681 atgaggcttg ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc
    19741 gtcttgataa tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc
    19801 aaattgccgc tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct
    19861 ggagcgatca gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc
    19921 tttgtcgtgg caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc
    19981 ttatcggtgc ggtcacgctg aatcagggcc gtgagattcg cccaattcgc aaatggatcc
    20041 agagcggcaa aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac
    20101 tgtaatcagg ataattagcg aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt
    20161 gtattacgtt taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgttatt
    20221 ttgattttgg agctgcccgc cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa
    20281 ttttcattat ttttggtttt cccaaaatga tgggctttga cggtacggtc caatatatgg
    20341 cctcgttggg cgcgccaatg ccgatgctgg cagcgattat tgcggtagtt atggaagtgc
    20401 ccgccgcgat attaatcgtg cttggctttt tcacccgtcc gctggcggtg ctgtttattt
    20461 tctacacgct gggtacggcg gtgattggtc accattactg ggatatgacc ggcgatgcgg
    20521 ttgggccaaa tatgattaat ttctggaaga atgtcagtat cgctggcgcg ttcttgctat
    20581 tggcaattac cggaccgggg gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc
    20641 ggcgtgaacg ccttatccgg cattttataa attacagcca ctctacccgc aacgacatct
    20701 ctgaggttgt tgattcccct ggtgctagcg caatgagatc acccccttcc gggcgatgat
    20761 gctcatccgg cgcatggctc atcggttcca gacagaagaa atcaaacgca tatcctttat
    20821 caaacgcagg gtcggaaaca aagatgaaat aacacggtgc aggtggcgtc gtttccatga
    20881 tgatggcata accttcctgc ggctgctcga tgcgggcctg accattccat ccggcaaaac
    20941 cattgttcac ccactgacgc ggcaacggcg tgagctggtt aaaatccagt tcctgcggta
    21001 gctgctcgca aaactcaccc gccagccact gctcccgttc cagccagtaa ccgctcgcct
    21061 gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg gtaccaaatg
    21121 gcagcgtctc tgccccttga ttggtgacag agagcgtcac cgtcagcgta tccgccgtta
    21181 aatgaaacgc ctgacttacc cgatagtgat agacaccgct gcgatgttca tacaccagac
    21241 acaaactatc atcgctgtgc gagacacatt gccactcgcc cagccagcca tcgccgtgta
    21301 gatagtgcgc atcccactca acattcggtt gcagttgata ctcacgcccc tgccagacaa
    21361 aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg
    21421 ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga
    21481 ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc gtgggataag gtatagatgg
    21541 tcatccgcta actccttaat ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc
    21601 catcagcaca taggcatcac ctgcctgttc tagcgagaag cgatgggtaa tggcgttacg
    21661 cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga acagactggt
    21721 cacccaggag ccgataatcc gccgttgatg gtgcatcaga tcagcgctga cctcgaattc
    21781 cacttttccg gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg attgcagtgc
    21841 cagcaagcga cctgcggcat taccggaaca atcgagcgca acatccgcgc caccgtgggt
    21901 aagttcagca attatctgcg gcagaccttc ggtggttgct aaatagccgt gatccatccc
    21961 ccctaactgt tttgccatcg ccagacgttc cggcagcata tcaacgccga tgatccgttt
    22021 tgcaccgcga cctttcgcca gcatcatcgc catcatgccg accggcccca gaccgaccac
    22081 cagcacgtta tcgctgccgg aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac
    22141 gccgcaactg ataaacgcac catcttcgta gctcagcgca tccggcagga ggatcagatc
    22201 tttttcttcc gccagcagat attcggcatg accgccgtca cgctgccagc cgtaagccgc
    22261 ttttcctttg ccagtacaag agataggaaa gccgcggcgg cagttcgggc aaaaaccaca
    22321 gccagaaatg tgatacacca gcacgcggtc gccctcttta aaatggcggc agccttgccc
    22381 catcgccaca atctgcccgc acggttcatg accgttgata aatccctggt ataacggttt
    22441 atcgggtgcc gccgccgtgg cacggtgttg atgatagata tagtggacat cgcttccgca
    22501 aatcccggag gatttcattt tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac
    22561 ttcccgcaga tcgacggttg aatttcctag taaataagca gccagcatcg ttttcataaa
    22621 tccctcatta acaatacgat taattttcat ccctgcagca cccgcgccag gggcgttaag
    22681 gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga tattcgccag caccgccacc
    22741 acgatgatga cgccgcgtac cacctgctgg aaaaaggagt taataccgag cagcaccaga
    22801 ccgttaccga ttagcgtaat caccagcaca ccaagcaatg taccgaacag ggaaccgcga
    22861 ccgccggaaa gtgccgtacc gccgaccacg accgcggcga tgacgtcaaa ctccagaccg
    22921 tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca acaaaatgcc ggtcaccgcc
    22981 gctaataatc ccgaaagggt aaagataaga atgcgcactc gacgaacgtt aataccgcac
    23041 aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg agcgcccgaa ggcggttttt
    23101 cggctaatga acacaaacag cgcaaacaac accatcatga tcagcgcgga tactggcaca
    23161 ccgagaaatt gtccgcccag ccagtccagc acctcgtttt cgtcaatcgg cactggcagt
    23221 gcgttggtca taaacagccc cattccgcgc agagcgctcc acagccccag tgtggcaacg
    23281 aaacttggta cgttaaacac gccgcgcagc accccggcga gcgttcccat cagcgcgcct
    23341 aacagcaaca ccagcagaca agcaatcgcc aacggaactt caaattgcag caaaaatgcc
    23401 aggcacaccg agacaaaagc caccatcggc ccaacgctga catcaatttc accggagata
    23461 ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc
    23521 atctggttat tcaatgagat aaagccaggc gcgttcaggg agaagaccag ataaagaatg
    23581 gcaatcacca ccagcaaacc gatctcatta atatggcgac tgacaaattg tttgagcgag
    23641 acgctcttgc cctgcggcaa tggtaatgac gaagcataca tcttcaggtt cctcggtaaa
    23701 attcatcagt gcacagacag aatggcggac atcagctcat ccacattgac cggcgagtga
    23761 aactcctgcg agaacgtgcc gtgctgtaat aacaggatgc ggtcacacac cagcggcaat
    23821 tcctccactt cactggagat aaacaccacg ctttttcctt cggcagccag ctcacggaca
    23881 atacggtaaa tctgctgttt ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac
    23941 aaaatctggc tggcggcata gacccaacga ccgatcacca ctttttgctg attgccacca
    24001 gaaagcgtgc cgatgggtgt ttcgctactg gcagccttga ccgtcatccg ctgcatcacc
    24061 tcttcggtca ggcggcggat ggtggaccat tgcagcacac cgttggtgct gattttttgc
    24121 cgattggtca gcactgtatt ttcgtcaacg cctagccagg gaatgatccc cgcttctttg
    24181 cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcatg
    24241 attttctcgc cgttgataac aatttcgccc tgttcatacg tctccagccc aacaatcgcc
    24301 ttcagcaatt cactgcgccc tgcccccagc aggccagcaa tgccaagcac ttcgccacga
    24361 cgtagcgtaa agctgatatc ctccagcttg ggcttatggc gtaacgcacg gacttccagt
    24421 acggcctgat ccacaatttc ctgaggggca accggcgcaa tatcaacgtg atcacgccct
    24481 agcattagcg acacaatatg atgcgtggag gtgttttcga gcatcacatc gcccgccacc
    24541 tgaccatcgc gcataacggt ggcataggag gcaatgcggc ggatttcttc catccggtgg
    24601 ctgacataaa tcaccgccac gcccagtgcc gacatctttt tcaccgcact gatcaccagt
    24661 tcaacttctg cactggcaag cgagctggtg ggttcatcaa gaatgaccac gcgcggctcg
    24721 cccttcatca cccgtgcaat ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca
    24781 agttgttcag gactaacgtc aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc
    24841 atttgcaggt aatcaatcat gccgttgcgg cgaggccatt gaccaaggca gaggttttct
    24901 gccaccgtca gcccttccac cagacttaat tcctgataaa ccgcacgaac ccccagttca
    24961 gcggcacggc gagtcaacgt agcttcgtca ccttccagtc gcgtctcgcc aatccagata
    25021 tcaccgctat ccgggcgttc gctgccggta agcattcgaa tgagagtcga tttgcccgcg
    25081 ccgtttttac ctaacagcgc acgaacttcg cctttattga gcgtgaagtt aacgttatcc
    25141 agcgcaacga cgccggggta acgcttattt cctgccacca cttttgctac cgggactgcc
    25201 tctgttgccg tgaacatagg gacctctgcg aatcagcgat tagggcagac catcaacgtg
    25261 cgttgccagc cactgtttac catcttccgt tttggtatag agatcgattg gcacctgaat
    25321 cactttttca ccgtcggctt gtttattgat aaccttcaat gtttgcgcga aaacagcatt
    25381 gcccattttc ttaccggaaa tatccactac cgctttcagc acctgattgt tttccagctc
    25441 ctgagcaatt tcggttgtca tatccgaacc gaaaacagca atttttccgg cctgattttg
    25501 attacgtacc gcttttaccg cgccgagtgt cgcaccgccc gactccccca taatggcgtt
    25561 gagatccggc gtggagataa tcagtttttc accaacggaa atcgctttat ctaaaacagt
    25621 cccttcctgg ttagcgacaa tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa
    25681 tcctttacgt cgctgcacac aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg
    25741 ctggtcaatt ttattggcaa taaaataatc ggcggcagcg ttacccagtt tttaccaaat
    25801 tccagtggat cgccgaccag atacgccgag acatatttat cgacaccctt ttgattaata
    25861 caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta
    25921 ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta
    25981 tcaacaaagg tactttcttt cgaaatatcc ccctgggcgt tagtttcaat taactgtacc
    26041 tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc
    26101 tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct ggcaaatagt
    26161 gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc
    26221 atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc
    26281 ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata
    26341 aaaggcatca cagtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat
    26401 tcagcgcgag cagactcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc
    26461 accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga taacaggtca
    26521 ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac
    26581 tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc
    26641 attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg
    26701 gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc atcgccctgc
    26761 gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg
    26821 ccttcgctta aattctccgg ataatacagc gcggcatgaa gcagttcgct ggcctgctgc
    26881 ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg
    26941 gtgaccttcc cttccccgcc ttcccacggg tggaatttac gcgtggcgag aatgtccgcc
    27001 gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg
    27061 cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct
    27121 ccacttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc
    27181 tgataagcat tatcaagata acgcgcggcc agctcataat cgtgtttctt attccacgca
    27241 tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc
    27301 cataaggcaa tggctttgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg
    27361 cgagcaaacc aacactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg
    27421 aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct
    27481 ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc
    27541 agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga
    27601 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga
    27661 ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc
    27721 tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag
    27781 caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc
    27841 gcgtcgaagt tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta
    27901 ctattgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    27961 cgttcgtaag cactggcgcg aatcaaactt gcttgtccac actgcggttt tttgttcagc
    28021 gcatgtgcac gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg
    28081 ttatattcca gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg
    28141 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    28201 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg
    28261 acgtccggca acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg
    28321 tcggcatcgg agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg
    28381 atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt
    28441 tcgcggatcg ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    28501 ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc
    28561 agagaatgat aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag
    28621 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    28681 ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt
    28741 tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga
    28801 caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc
    28861 acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg
    28921 gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc
    28981 ggtgggaaaa cgctctgatg cccttcaccc cctttcactg ccgggttgac ccaccacaag
    29041 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg
    29101 tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg
    29161 acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    29221 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc
    29281 agcatcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc
    29341 actttatccc atgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg
    29401 tagtcgtttt ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta
    29461 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    29521 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    29581 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    29641 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    29701 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    29761 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    29821 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    29881 aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    29941 tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt
    30001 gtcaggttgc taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg
    30061 taccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    30121 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga
    30181 ccggcgtatc gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc
    30241 tgctggaaga gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc
    30301 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    30361 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    30421 gcggacagat tggtcacacc atcgttaatc cggatggagt cgtctgcgac tgtggacgtt
    30481 acggcagcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    30541 taaaatcaca accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa
    30601 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    30661 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    30721 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    30781 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    30841 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    30901 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata
    30961 aacgaaaaat tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg
    31021 aatttgcaag attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga
    31081 gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt
    31141 cgagaacttt acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt
    31201 cacacatcca gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg
    31261 ccggagtacc tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc
    31321 tgtttttgtt gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca
    31381 ggtttttatc aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt
    31441 tgtagccgcg ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct
    31501 ggtaagtttt gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta
    31561 cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca
    31621 gctcttcgct accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt
    31681 gagtggtggt agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa
    31741 ccaggcccgc aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt
    31801 cacgcatttt cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt
    31861 tcggcttgtg ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat
    31921 cgataccgta aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag
    31981 tcaggtgacc gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg
    32041 cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag
    32101 cgccgaacag ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc
    32161 aaccgccgta gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac
    32221 cctgcgcctg cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct
    32281 cttcctgacg tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa
    32341 tgttcatttc acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc
    32401 ctttataggc ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac
    32461 aggtcttgac aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga
    32521 ataaagagct tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact
    32581 tcatcacatt ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat
    32641 aagatgcatt tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct
    32701 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    32761 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    32821 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    32881 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    32941 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    33001 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    33061 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    33121 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    33181 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    33241 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    33301 cagtactctt tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa
    33361 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    33421 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    33481 atctctaccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    33541 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt
    33601 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    33661 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    33721 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    33781 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    33841 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    33901 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    33961 cgaatgaccc gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    34021 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    34081 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    34141 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    34201 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    34261 atgctattcc ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    34321 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    34381 gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc
    34441 agcttacgca agtagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc
    34501 gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc
    34561 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg
    34621 gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct
    34681 gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt
    34741 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    34801 cgcgccatcg cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca
    34861 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    34921 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    34981 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    35041 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    35101 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    35161 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    35221 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    35281 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    35341 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    35401 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    35461 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    35521 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    35581 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    35641 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    35701 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    35761 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    35821 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    35881 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    35941 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    36001 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    36061 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    36121 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    36181 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    36241 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    36301 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    36361 attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg
    36421 actgcgcgga gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg
    36481 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    36541 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    36601 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    36661 accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc
    36721 tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa
    36781 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    36841 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    36901 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    36961 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    37021 taatcaagca gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc
    37081 accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg
    37141 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    37201 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    37261 gagcgtaact cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc
    37321 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    37381 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    37441 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    37501 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    37561 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    37621 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    37681 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    37741 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    37801 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    37861 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    37921 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    37981 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    38041 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    38101 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    38161 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    38221 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    38281 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    38341 cttattagat tgcaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    38401 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    38461 gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact
    38521 gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat
    38581 gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc
    38641 gaccactttc agtcgtaacc gccgtaatac cgttcggtga accgttcggg ttagccgggt
    38701 aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt
    38761 ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag
    38821 caatcggcat ttgcgagccc accatcccct gcaacagcag agacgggctt tgggttactt
    38881 caaccaggct gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca
    38941 actcactccc cgggatcagt tcacgcaggt tagacatcat ctggcaaccg ttacataccc
    39001 ccagggccag cgtttgcgga cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat
    39061 tgaacaggat agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac
    39121 ctccgcacgc gaccagggcg tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc
    39181 tcatatgcac gtcgatagca tcaaagcccc cacgttggaa agctgccgcc atttcaacat
    39241 gcgagttcac gccctgctca cgcagtacgg caactttcgg acgtgcgcca gtggcaatat
    39301 acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg
    39361 cgtcgttaga tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct
    39421 gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata
    39481 cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat
    39541 ggacgcaatc cgcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg
    39601 cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac
    39661 ccagagaggc gatatccgcg tcaatgccac aatgacctgc aaaggccatt tccgccagcg
    39721 ttaccagcag gcctccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca
    39781 gcgcctgaat cgcgtcatag aagcctttca gttggacaac atcgcgtaca tctgccggtt
    39841 tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt
    39901 tgcccaaatc aatcagcagc agtgcgttat cttcggtaca aagctgcggc gtgatggtgt
    39961 gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt
    40021 cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg
    40081 ggatcgtcag gcccagcgcc ggacaaagtt cttcacccac ggctttaacg gcttcataca
    40141 ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc
    40201 gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac
    40261 gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt
    40321 caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc
    40381 acggccccac catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca
    40441 ccaggaaagt tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa
    40501 tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg
    40561 tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa
    40621 aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg
    40681 gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca
    40741 gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt
    40801 cgcttagaat atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg
    40861 gcatcgcgtt agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat
    40921 caccaagctg ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt
    40981 cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg accagacgcc atggaagacg
    41041 ccgcgccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac
    41101 cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca
    41161 gcatgatcgg tttgtgataa ccgcgcagct cttcgccatt gtggctgttc actttttctt
    41221 cataagtacg gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac
    41281 cgcccagcgg gccttcggtc atgatgtcca gcgcggtaac aatgcgctca ggcttaccga
    41341 aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggatacg gagaaaccaa
    41401 ccaaaccagc tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc
    41461 cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga
    41521 ctttcatcag gatgtgcgcc ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag
    41581 caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga
    41641 gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat cctgaacagc gatttcggct
    41701 gttgttcacc gtcgataatc cagtcggcgt taaaaatttt gtggcggcag tgctcagagt
    41761 tcgcctgagc gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag
    41821 catcctgcag atagtcaatt tcatcttccg ccagagccaa gccaagacgc aggttagcgt
    41881 cgatcagcgc ctgacggccc tgccccagca aatcaacgct tgtgaccgga gtcggttgat
    41941 ggtgggcaaa caactgctct gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt
    42001 cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat
    42061 agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat
    42121 cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt
    42181 tgccttgcgg ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg
    42241 cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa
    42301 tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg
    42361 ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt
    42421 cggtgtaccc aagggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa
    42481 ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt
    42541 aataagttgc catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt
    42601 cactattatt acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa
    42661 aaaattaaag attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct
    42721 ctggccatcc attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg
    42781 gggagagttg cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa
    42841 accttttggc ctggattacg aactggcgaa acagtttgcc gattacctcg gcgtaaaact
    42901 gaaagtgacc gtgcggcaga atatcagcca gctgtttgac gaccttgata atggtaacgc
    42961 cgacctgctg gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg
    43021 ccctacctat tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg
    43081 tacgctgggc aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtagttaa
    43141 cgatctccag accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa
    43201 aaaaggctct gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc
    43261 tgattctgtc gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga
    43321 tatcaccgat gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc
    43381 cgccgccctg ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga
    43441 agagaaatac ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg
    43501 cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat
    43561 tgactggcgt ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac
    43621 ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg
    43681 cattacggat cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga
    43741 tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc
    43801 tgcgtacaat atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa
    43861 agggaatcct gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc
    43921 ctattacagc aagctgactt acggctacgc tcgtgggcat gaagcctacg cttatgtcga
    43981 aaatattcgt aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc
    44041 tacagaagcg gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg
    44101 caaagagaaa tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca
    44161 ttctccctct ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga
    44221 ggattgcgct tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag
    44281 caacgccgcg cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc
    44341 cggatgatgc agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc
    44401 aaagaccacg cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc
    44461 aagcgtgaca tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg
    44521 ccgcagggcc atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca
    44581 gccttcgccg attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc
    44641 atcccaggca cgtttcgcca gcgtcagcgc gtgacgcatc caatattcgt ggctaaattc
    44701 gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta
    44761 tgctttactc tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg
    44821 acagaaataa agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag
    44881 cggcgtgccg attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt
    44941 caatacccaa ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag
    45001 ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca
    45061 gatatcagca tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc
    45121 ggtaacattg tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc
    45181 gctgtgacca aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc
    45241 ttttatcaat aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag
    45301 cggttggcga cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt
    45361 tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg
    45421 actgaaacat cctgattaat tttcgctact acatagccta acagatagat catcactttt
    45481 ccggcaacaa tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc
    45541 aatggaatat aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc actatttcat
    45601 cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag cgttaccaag ggcttgccca
    45661 gagtgataaa aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag
    45721 ctctcagcaa ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca
    45781 aaaactcggc tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag
    45841 ccagccggaa tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg
    45901 gctggtcggc gaaaaactga ttaaagaaaa taccgccgct atgtacgcaa cgctaaacgt
    45961 taatagtgaa gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat
    46021 tctgaccggt attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa
    46081 gattggcttc aatgctgccg cactgcgcga tatgcatgcg ctactcgcaa cagtacaggc
    46141 gtcgtcccct gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa
    46201 cctggcggca gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac
    46261 tccgaatgcc ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc
    46321 gacaaacagt gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt
    46381 cattgcgctg attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc
    46441 gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt
    46501 gttttgagag tttccttatg gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt
    46561 gtgaaatcaa ataaaaacac atttattttc atagtgatac agcaatgtgt tattgaatgt
    46621 gttgttggat ttatggtgca aaaaaatttt gcataagttc agcaacacaa agaacaccac
    46681 tttctttaac gtcctccgcg ccctaaagga cggggcttta cggcgcaaaa ggcctgtgac
    46741 atacaacatt aacttttcag tcaaccgtag cacgttcttg tatacgccgt gtctgcggtt
    46801 ttatataacc ctggctggaa acctcttata caaagtcgat tcgccaacat catagatgat
    46861 cgccaccttc tggcgagaaa ctcctgatgc aattagtcgc ccggcctgcg cccattattc
    46921 tggtgaaagt ttgggacggc gaccgccaat tcgtccctgt gcgcgagcag cttccagtcc
    46981 tgcttttgtt cgttcaacaa tcagttcacg ttccatttca gccagggcac ccatcacatg
    47041 gaagaaaaag cgtcccattg gtgtgctggt atcaattgaa tcagtcagac tacgaaagtt
    47101 gatgcctcgt tcgcgcaacg cctccaccag cacgacaaga tgccgcatac tgcgccccag
    47161 ccgatccagc ttccagacaa ccagtgtgtc gcctgccgat aatgtcttga gcagtttttt
    47221 cagccccggt ctttcggact ttgtaccact tatcttgtct tcaaaaatca gctcgcatcc
    47281 tgcacagttc aacgcattac gttgtagatc ggtattctgg tcatttgttg atacgcgtac
    47341 atagccaa
//
LOCUS       DAJTYH010000019        46303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTYH010000019 DAJTYH010000000
VERSION     DAJTYH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(133..208)
                     /locus_tag="OO609_001080"
     tRNA            complement(133..208)
                     /locus_tag="OO609_001080"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Lys,seq:ttt)
     gene            complement(373..1164)
                     /gene="cpoB"
                     /locus_tag="OO609_001081"
     CDS             complement(373..1164)
                     /gene="cpoB"
                     /locus_tag="OO609_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6632582.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1174..1695)
                     /gene="pal"
                     /locus_tag="OO609_001082"
     CDS             complement(1174..1695)
                     /gene="pal"
                     /locus_tag="OO609_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6632583.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1730..3022)
                     /gene="tolB"
                     /locus_tag="OO609_001083"
     CDS             complement(1730..3022)
                     /gene="tolB"
                     /locus_tag="OO609_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6632584.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3155..4396)
                     /gene="tolA"
                     /locus_tag="OO609_001084"
     CDS             complement(3155..4396)
                     /gene="tolA"
                     /locus_tag="OO609_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6632585.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4461..4889)
                     /gene="tolR"
                     /locus_tag="OO609_001085"
     CDS             complement(4461..4889)
                     /gene="tolR"
                     /locus_tag="OO609_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6632586.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4893..5585)
                     /gene="tolQ"
                     /locus_tag="OO609_001086"
     CDS             complement(4893..5585)
                     /gene="tolQ"
                     /locus_tag="OO609_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6632587.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5582..5986)
                     /gene="ybgC"
                     /locus_tag="OO609_001087"
     CDS             complement(5582..5986)
                     /gene="ybgC"
                     /locus_tag="OO609_001087"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6632588.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6136..6429)
                     /gene="ybgE"
                     /locus_tag="OO609_001088"
     CDS             complement(6136..6429)
                     /gene="ybgE"
                     /locus_tag="OO609_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6632589.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6429..6542)
                     /gene="cydX"
                     /locus_tag="OO609_001089"
     CDS             complement(6429..6542)
                     /gene="cydX"
                     /locus_tag="OO609_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6632590.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6557..7696)
                     /gene="cydB"
                     /locus_tag="OO609_001090"
     CDS             complement(6557..7696)
                     /gene="cydB"
                     /locus_tag="OO609_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6632591.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7712..9280)
                     /gene="cydA"
                     /locus_tag="OO609_001091"
     CDS             complement(7712..9280)
                     /gene="cydA"
                     /locus_tag="OO609_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6632592.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10129..12762)
                     /gene="mngB"
                     /locus_tag="OO609_001092"
     CDS             complement(10129..12762)
                     /gene="mngB"
                     /locus_tag="OO609_001092"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6632593.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12780..14696)
                     /gene="mngA"
                     /locus_tag="OO609_001093"
     CDS             complement(12780..14696)
                     /gene="mngA"
                     /locus_tag="OO609_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6632594.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14866..15588
                     /gene="mngR"
                     /locus_tag="OO609_001094"
     CDS             14866..15588
                     /gene="mngR"
                     /locus_tag="OO609_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6632595.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15691..16560)
                     /gene="sucD"
                     /locus_tag="OO609_001095"
     CDS             complement(15691..16560)
                     /gene="sucD"
                     /locus_tag="OO609_001095"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6632596.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16560..17726)
                     /gene="sucC"
                     /locus_tag="OO609_001096"
     CDS             complement(16560..17726)
                     /gene="sucC"
                     /locus_tag="OO609_001096"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6632597.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17820..19037)
                     /gene="odhB"
                     /locus_tag="OO609_001097"
     CDS             complement(17820..19037)
                     /gene="odhB"
                     /locus_tag="OO609_001097"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6632598.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(19052..21853)
                     /gene="sucA"
                     /locus_tag="OO609_001098"
     CDS             complement(19052..21853)
                     /gene="sucA"
                     /locus_tag="OO609_001098"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6632599.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22154..22870)
                     /gene="sdhB"
                     /locus_tag="OO609_001099"
     CDS             complement(22154..22870)
                     /gene="sdhB"
                     /locus_tag="OO609_001099"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6632600.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22886..24652)
                     /gene="sdhA"
                     /locus_tag="OO609_001100"
     CDS             complement(22886..24652)
                     /gene="sdhA"
                     /locus_tag="OO609_001100"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6632601.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24652..24999)
                     /gene="sdhD"
                     /locus_tag="OO609_001101"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24652..24999)
                     /gene="sdhD"
                     /locus_tag="OO609_001101"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6632602.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24993..25397)
                     /gene="sdhC"
                     /locus_tag="OO609_001102"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24993..25397)
                     /gene="sdhC"
                     /locus_tag="OO609_001102"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6632603.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25778..25885
                     /locus_tag="OO609_001103"
     CDS             25778..25885
                     /locus_tag="OO609_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632604.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26091..27374
                     /gene="gltA"
                     /locus_tag="OO609_001104"
     CDS             26091..27374
                     /gene="gltA"
                     /locus_tag="OO609_001104"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6632605.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27765..28331
                     /locus_tag="OO609_001105"
     CDS             27765..28331
                     /locus_tag="OO609_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6632606.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28382..30841
                     /locus_tag="OO609_001106"
     CDS             28382..30841
                     /locus_tag="OO609_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6632607.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30856..31587
                     /locus_tag="OO609_001107"
     CDS             30856..31587
                     /locus_tag="OO609_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6632608.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31584..32660
                     /locus_tag="OO609_001108"
                     /pseudo
     CDS             31584..32660
                     /locus_tag="OO609_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32812..33858
                     /locus_tag="OO609_001109"
     CDS             32812..33858
                     /locus_tag="OO609_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6632609.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(33855..34646)
                     /gene="nei"
                     /locus_tag="OO609_001110"
     CDS             complement(33855..34646)
                     /gene="nei"
                     /locus_tag="OO609_001110"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6632610.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34682..35416)
                     /gene="pxpA"
                     /locus_tag="OO609_001111"
     CDS             complement(34682..35416)
                     /gene="pxpA"
                     /locus_tag="OO609_001111"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6632611.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35406..36338)
                     /gene="pxpC"
                     /locus_tag="OO609_001112"
                     /pseudo
     CDS             complement(35406..36338)
                     /gene="pxpC"
                     /locus_tag="OO609_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(36332..36988)
                     /gene="pxpB"
                     /locus_tag="OO609_001113"
     CDS             complement(36332..36988)
                     /gene="pxpB"
                     /locus_tag="OO609_001113"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6632612.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(37011..37754)
                     /gene="ybgI"
                     /locus_tag="OO609_001114"
     CDS             complement(37011..37754)
                     /gene="ybgI"
                     /locus_tag="OO609_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6632613.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38025..39506
                     /gene="dtpD"
                     /locus_tag="OO609_001115"
     CDS             38025..39506
                     /gene="dtpD"
                     /locus_tag="OO609_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6632614.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39656..41074)
                     /gene="phrB"
                     /locus_tag="OO609_001116"
     CDS             complement(39656..41074)
                     /gene="phrB"
                     /locus_tag="OO609_001116"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6632615.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(41071..41580)
                     /locus_tag="OO609_001117"
     CDS             complement(41071..41580)
                     /locus_tag="OO609_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6632616.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            41709..41996
                     /locus_tag="OO609_001118"
     CDS             41709..41996
                     /locus_tag="OO609_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6632617.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(42051..42431)
                     /locus_tag="OO609_001119"
     CDS             complement(42051..42431)
                     /locus_tag="OO609_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632618.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(42434..45820)
                     /locus_tag="OO609_001120"
     CDS             complement(42434..45820)
                     /locus_tag="OO609_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6632619.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(45839..46042)
                     /locus_tag="OO609_001121"
                     /pseudo
     CDS             complement(45839..46042)
                     /locus_tag="OO609_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        11120 a        12609 c        11187 g        11387 t
ORIGIN      
        1 actacgctgg atactacaac taactatgtt agtgggggtc gtgcaggatg actcgacttc
       61 gtctcgccct tcggcgccgt tgctgatgca acgttatcct tcacgtttta ctaccgcttc
      121 caccttagcg attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
      181 actgctctac caactgagct aacgacccac tttttcgttg ctttcggttt gtttgatatc
      241 ccgtggcaac ggcggcatat attactgatt tcagacttga gcgcaacaaa aatttcgatg
      301 cagatcactc aactgcttat gattcgcacg acacgaccag aaataatgcg acttctggtc
      361 gtgtgttatg cattacatcg cgttcagacg tttttgtgcc tgtttagcgc catcagtacc
      421 agggtattta ctgataacct gctggtatac ggctttcgct tttgcggtgt cacctttgtc
      481 ctgcatgatg acgccgactt taaacatcgc atcagcagcc tttggtgact tcggatagtt
      541 tttcactacc gaagcaaaat agtacgccgc atcatctttt ttacccttgt tgtagtttaa
      601 ctgacccagc caataattgg cgtttggcag gtaagttgaa tcagggtaat ttttgatgaa
      661 attctgaaat gccaccattg cgtcatcctg gcgggattta tcctgcacca gcgcaatagc
      721 tgcattgtaa tccgtgtttg catcgccgct tttcaccggc gcgccagcat tcgcagtacc
      781 agcatcagct gtcggcgtcg ttgatgccgt cgcaccgctt tgatcgctgc tggttgattg
      841 cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac aggatctgct tttgccgctc
      901 cacgacctga ttcagttgat actgattttc ctgaatctga ccacgcaggg aatcaatatc
      961 ggattgatta tcagaaagtt gttgctggag ttgggttaaa agctggctgt gagcattaga
     1021 aatacgctca agttgagtga cgcggtcttc gaccgagcct gagctgacac tactgattgg
     1081 tgcctgagca aaagcggccc agggggccgc tataccaacc agtaacgaca gactcaatag
     1141 ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa accagtaccg cacgacggtt
     1201 tttggagtat gccgcttcgt catgacccag tactgcaggt ttttctttac cgtaagaaac
     1261 gatggagatc tggtctgcag aaacgccttt accctgcagg tacatcttaa cggcgttcgc
     1321 acgacgttca cccagggaga tgttgtattc cggagtacca cgttcgtccg cgtgaccttc
     1381 tacggtgact ttgtaagacg ggttgctacg caggaagttt gcatgtgcat ccagcatttg
     1441 agcgaagtca gaacggatat cgtacttgtc cagatcgaag taaacgatgt tgttctgctg
     1501 cagctgttgc atttgcagac gagcctgctc ttcggaagac atgttgccgt tgccgccgtt
     1561 cgcatccata ccagtgccgg cacccagcat gccttcgctg ccgtcattgc tggcgttctt
     1621 gttggaagaa catgccgcaa ttgccataac aggcagagca atcatcagcc ctttcagcac
     1681 tttgttcagt tgcatttcaa tgattccttt actattcaat taattattat cacagatacg
     1741 gcgaccaggc agggaatttg acctgtccat cagttgccgg aagacgcgct ttgaaacgcc
     1801 catctgtaga aaccaaattc agcacggatc ccatcccctg agaagagctg tagattacca
     1861 tagtgccgtt aggtgccaga cttggcgttt catccaggaa cgtggacgac agaacttgta
     1921 cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg cccaccattg gagctgacca
     1981 ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg gttctgcgaa ccttcccagg
     2041 taatacgttg tggcgcaccg ccgttgatat tcactttata aacctgcgga cgaccggcct
     2101 ggtcagaagt aaatgccagg ttctggctat ccgggaacca ggtcggttcg gtattgttac
     2161 tgcgaccatc agtcacctgg cggatctgac cagaagccaa atccattacg tacaggttca
     2221 gactaccggt tttcgacaag gcgaatgcca gtttgctgcc gtctggcgag aatgcaggtg
     2281 caccgttgtg acgcgggaat gaagccacct gacgtacagc gccatttgcc agcgtctgaa
     2341 taaccagcgc ggaacgaccg ctttcgaagg tcacataagc cagtttagaa ccgtctggtg
     2401 accacgccgg agacatcagc ggctgcggtg aacggtgaac gacaaactgg ttgtaaccgt
     2461 catagtcaga tacgcgcagt tcatacggga actgaccgcc gttggtctga acaacgtagg
     2521 caatacgggt acggaacgca cctttaatgc cggtcagttt ttcaaacact tcatcactgg
     2581 cggtatgacc agcataacgc agccactgct tgttcacttt gtacgagttc tgagcaagta
     2641 cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc aacattgtaa gaaccatccg
     2701 gattcggagt gacttgaccg acaactacag cgtcaatgcc cagtgcggac catgcagctg
     2761 gttgtacttc ctgcgcacta cccggctgct gtggcagacg agcgcgatct aacggattaa
     2821 atttaccgct gttacgcaag tcagcagcaa cgatgccgcc aatatcttca ggtgccgcac
     2881 caggccccgc ccactggaaa ggaacaacac caataggacg accggaatct acaccgctgt
     2941 cgatcacaat gcggacttca gcatgcagaa ctgatgccca cagtatgaga aaaccaaatg
     3001 ctactcgtaa tgcctgcttc atcatatctc ccttatctgg accgatggcc cacgataatt
     3061 tagcagaatg ttaacaaact aaaatacaca aaactaccag aaccccgtgg caaccggtgc
     3121 ctgatgttga ccgtccgaac agtcaacatc gcgattacgg tttgaagtcc aatggcgcgt
     3181 ttttgaacac ttcatatact gcctggcttg gtggtttcgg gatcttcgca agtttagctg
     3241 ctgccaacgc agcctgacaa agtgcgggat cgccaccttc aggtttgata tccagtaaca
     3301 taccatcggg tgccagtttt atgcgcagcg tacaggtttt gcctgcatag gacgatgcgt
     3361 catagaactt actttcgata gcagatttaa tctgcccggc atagttattg atatcggccc
     3421 ctgatgcgcc attgttttta gtattaccac tcccggcagg cgaagcattg ttccctttcg
     3481 cccctccccc cgttttcggt gcattcttac cagagcttag ctcaccgaaa atatcatctg
     3541 cctctgcggc agcttttgct gcagcggctt tttcagctgc tgcttttgcc gctgccgctt
     3601 ttttgtctgc ggcggctttt tctgccgctg ctttcttatc agctgctgcc ttatcagcag
     3661 ccgctttttc tgctgctttt ttgtcggctg cagctttctc tgctgccgct ttcttatcag
     3721 ctgcagcctt ttcagcagcc gctttcttct cagcttctgc cttggctttt tcagcagctt
     3781 cagttgccgc tttctttctt gcttcagctg cagctgcttc tgccgcttcc gctttcttct
     3841 tcagtgccgc agcggctacc tcggcttttt tctgcgcttc ggctgcggct ttggcggctt
     3901 ctgcttctgc ttttttcttc gcgtctgcag ccgctttctt cgctgcttct tccgcagctt
     3961 tagcatctgc cgccgctttc gctgccgcca cttcagcttg cttctgcttt aactcggcct
     4021 gttttgcggc ttcttcagcc tgctttttct gctcctgagc cgctaaccgc tctttctcaa
     4081 gttgcttcag gcgttcctgt tcagccgctt gtttctcacg cagttcttca gcagcctgct
     4141 gttccttcat cttgcgctgt tcatcagaac gcttcgcgct tgattcctgg ctttgcatgc
     4201 gtttgtactg ctcaactacc gcacctgaat caaccatgac agcgtcgatg gacgaaccac
     4261 cgccgcctcc ggctgaagct tctatattct catcgaacga actccagatc agcgccgcaa
     4321 ataagatgac atgcagcact gctgaaataa ttatcgcccg cttgagcttg tcgttttgtt
     4381 cggttgcctt tgacactctc ggtttccaaa aactgttcgc ctgttacccg ctctctttca
     4441 agcaagggaa acgcagatgt ttagataggc tgcgtcatta aaccaaccga tttcacaccc
     4501 gcactatgta acaagttcag tgctttaatt atttcatcgt aaggcacatc ttttgcgcca
     4561 ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc tggacacttc cgccaccacc
     4621 tgctctggtg gtaaacgctc caggcgatct ttctcaacca ccacggtgta ctgaccaata
     4681 ccagacactt caacaatcac tggcggatta tcgttactgc tcaccgcctg tgattcagta
     4741 gcgtctggca gatcgacctc cacgctctgg gtgatgatgg gcgctgtcgc cataaagatc
     4801 agcaacagca ccagcagtac gtccagcaac ggaacaatgt tgatttcgga cttgagatcg
     4861 cgacgacctc gtccacgcgc tctggccatg gcttacccct tgttgctctc gctaacggta
     4921 aacgcctggc ggtgcagaat cgcggtaaac tcttccataa agttgtcgta attcagttcc
     4981 agtttgttta cgcgctggtt gagacggtta taagccatta ccgccggaat tgcggcgaac
     5041 agaccaattg ccgtcgcaat caacgcttct gcgataccgg gcgcaaccat ttgcagtgtt
     5101 gcttgtttta ccgccccgag ggcgataaag gcgtgcatga tcccccagac cgtaccaaac
     5161 agaccaatat acgggctgat ggagccaacc gtaccgagga acggaatgtg cgtttccaga
     5221 ttttcaagtt cacggttcat ggagatacgc atagcacgcg acgccccttc cactacggct
     5281 tccggcgcat ggctattggc acgatgcagg cgcacaaact ctttgaaccc gctgtaaaag
     5341 atttgttccg aaccagtcag attatcccgt ttcccctgac tctcttgata gaggcgagag
     5401 agttcgattc cagaccagaa tttatcttca aacgcttcgg cttcacgcgc cgctgcgtta
     5461 agaatacggg ttcgctggat aataatggcc caggatgcga ttgaaaaacc aatcaaaatc
     5521 aacatgataa gtttaaccag aaggctagcc ttcaggaaca aatcaaggat attcatgtca
     5581 gtcactgctt aaactccgcg acaatagact tgggaagcgc acgaggcttc attttgagtg
     5641 ggtcaacgca aacaaccaga acctctgctt cattcagcaa agtattctcg gcgttgacaa
     5701 tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt tatttcagtc tgtatttcga
     5761 gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt cattttacgt accacaaagg
     5821 caacgcgttc agccatcagc gcctgctgac tgaagtgatg atgacgcagc atctctgtgc
     5881 gtgctctttc ataaaaagcg acgtaactgg cgtggtacac cacaccaccg gcatcggtat
     5941 cttcatagta gacgcgaacc ggccatcgaa acagcgttgt attcacttta catcccggta
     6001 atgcaacaaa agttagagct tttaaacgct gctactatac gcgcgcgatt gaggtttggg
     6061 aatgagggca agttaagggc gcgtaaaaat ttatgggcct agagcggccc ataatttttt
     6121 aaagagaatt ctgatttaaa agaagaagaa aatcagcccg acaatgagaa caatatcggc
     6181 aagcaatggg caaaaaatcc cttgccaaag aaccttctgc ggacgaaagc ccacgccgtg
     6241 aatcacacca gcacagacgg cccacatcag caataaacca tgccagattt ccagttcact
     6301 ggtcttcgcg gcaaaacgag atgggtccca aaacatacat cctgctaaca gaagcgccat
     6361 cacgaaggaa agcgcccgca gggggcgctt gtccattacc gcatacaagg tcgcgataat
     6421 cttactcatc agatgtcttc ttgaccggct ttgcctgatt cgacgtgttc aagcgccagc
     6481 gcggtgatta ccccaaacga acaggcaaga agcgttccca gaatccatgc gaaataccac
     6541 atttttagct ccttacttag tacagagagt gggtgttacg ttcaatatct tctttggtga
     6601 tacgaccgaa cattttccag taacaccagg cggtgtagag cagaatgatc ggtaccagaa
     6661 ccaccgcaac ccaggtcatg acgttaagcg tcagctggct ggaagttgca tcccacattg
     6721 tcagacttgc gttcatcatg gtgctggacg gcatcacaaa cgggaacatt gcgataccgg
     6781 ctgtcaggat gatgcaggcc agcgtcaggg aggagaacac aaacgcccac gcggctttat
     6841 ccatacgtgc agtcaggatg gtcagcagca gcagaaccac acccagtgcc ggaatagccc
     6901 acagaattgg cgtgttgttg aagttaacca gccatgcgcc agcttcacga accacttctt
     6961 tattcagtgg gttagaggct gcgtaatggt ccattgtcga tttcacgaca taaccatcga
     7021 taccgtacat cacccatacg ccagccagtg cgaaacagac cagtgtcacc agcgcagcca
     7081 cctgagccgt tgcacgggta cgcaggtgca gttcgcccac ggtacgcatt tgcagatagg
     7141 ttgcgccctg agtaatgatc atccctacgc tcaccacgcc tgccagcagg ccgaacgggt
     7201 taagcaactg gaagaagtta ccggtgtagt acagacgcag atattcatca acgttgaacg
     7261 gtacgccctg caacaggtta ccgaacgcta caccaattac cagcggcgga acgaagctac
     7321 caatgaagat gccccagtcc cacatgttac gccagcgggt ttcttcaatc ttggagcggt
     7381 agtcaaaacc gaccggacgg aagaacaaag acgccagcac gaggatcatc gccacataga
     7441 agccggagaa cgcagcggca tagaccatcg gccaggcagc aaagagtgcg ccgcccgcgg
     7501 tgatcagcca aacctggtta ccgtcccagt gtggtgcaat ggagttaatc ataattcgac
     7561 gctcggtgtc gttacgaccg aggaaacggg tgagcatgcc cacccccatg tcgaaaccgt
     7621 cagtgactgc aaaaccaatc agcagaacgc caaccagcag ccaccagata aaacgcaata
     7681 cttcataatc gatcatttga cgactcctgt cttagcgtgc cggctgagta gtcgtggaag
     7741 actgctcaaa gtgatagcga ccggttttca ggctgcttgg gccgaggcgt gcaaacttga
     7801 acattaagaa caattctgcc accaggaaca gggtatacag gccgcaaatc agcaccattg
     7861 agaagatgag atcgcctgcg gtcagtgacg aattcgccac agctgtcggc agcacttcac
     7921 cgatagccca cggttggcgg ccatattcag ccacgaacca gcccgcttct acagcaatcc
     7981 acggcagcgg aataccgtac agcgcggcgc gcagaagcca ttttttctcg ccaatgcggt
     8041 tgcggatgac actccagaaa gagagcgcga tgattgccag cagcaggaag ccacacgcca
     8101 ccatgatacg gaacgcaaag tacagcggcg ctacacgcgg gatggagtct ttggttgcct
     8161 gttgaatctg cgcttcagtc gcatcagcca cgtttggcgt atagcgtttc agcagcagac
     8221 cgtaaccgag gtctttcttc atgctattga actggtcacg aaccgtctgg tcggtagaac
     8281 cagaacgcag ttgttcgagc agagagtacg ccttcatccc gttacgaatg cgttcttcat
     8341 gctgcaccat cagctctttc aggccgataa ccggggtatc cacggaacgc gttgcaatga
     8401 tgcccagtgc gtaagggatc tgaatcgcaa atttgttcgt ctcctcttcc tgatcaggaa
     8461 tgccgaacaa agtaaaggca gcaggcgcag gttgcgtttc ccactcggct tcaatagcag
     8521 ccagtttggt tttctgcacg tcgcccattt cgtagccgga ttcatcaccc agaacaataa
     8581 cagacagaac agcagccata ccgaagctgg cagcgatagc aaaggagcgt ttagcgaagg
     8641 cgaagtcacg acctttcagc atataccatg cgctgatacc gaggatgaac atcgcgccag
     8701 tcacataacc agacgctaca gtgtgaacga atttcacctg agcaaccggg ttaagcacca
     8761 gctcggagaa gctcaccatc tccatacgca tagtttcaaa gttgaaatcg gacgcgattg
     8821 ggttttgcat ccagccgttc gcaaccagaa tccacagtgc ggacaggttt gaaccgagcg
     8881 ccaccagcca ggtgacacac atatgctgaa ctttacccag acgatcccaa ccgaagaaga
     8941 acagacctac aaaggtggat tcgaggaaga aggccatcag accttcgatt gccagcggcg
     9001 caccgaagat atcccctaca tagtgggaat agtaagacca gttagtcccg aactggaact
     9061 ccatggtcag accggtagcc acacccagag cgaagttgat accaaacaac ttgccccaga
     9121 acttggtcat atctttataa atctgtttgc cggagaggac gtagaccgtt tccataatgg
     9181 ccaggaggaa cgccataccg agcgtcagtg gcacaaaaag gaagtggtac atcgcggtca
     9241 aggcaaactg taagcgcgac agttcgacta tatctaacat catgactcct tgctcatcgc
     9301 atgaagactc cgagagtgaa ccccgttaga aagggtcaca cgcatgcccc aatagaaatt
     9361 tgtttgaaca ctttcatcat accatgtgaa tgcacacaaa aacggcgtaa agaaaggttg
     9421 caaacatgtt aataaaaact caaattgatc ccacgtatat attacgccgc aaaatcctta
     9481 caataaacag gtttttgttg aagcaaattt gcatttttcg acggtgatca atttatagct
     9541 aaattgccgc ctttcagcca atttgatcga gaacaattta tctctttttg atgcccattt
     9601 ccaagactta tacattgata aatatcaatt tctacaaatt atgttaatca tgtttcaaca
     9661 aggtaacttt agtgatattt atgtttaaaa tgtgtgaatt ataagactga aattagtttc
     9721 ggttggcaat taaagataaa aagagacgaa gcaaaaacag cacggttgac cttaattctt
     9781 tttttggtca ctggctaata cgacgcagag tttcaggatt attaataaag gcataaatct
     9841 ccctcccgcc gatattaaca tttaattaac ttataattat attataatta actcacccgt
     9901 ggttattatt ttgttaaaaa taaagccaca tatacgtagc ttgagagtga agataaacac
     9961 aaaattacct gatgcgctac gcttgccagt gtctacatca tctctgcaat atattgaatt
    10021 tgcctgcttt tgtagaccga ataaagcgtt catgccgtat ccggcatgaa caaagcgccc
    10081 tttgtcaaca atctggccgc gcatatgtgg cttcggtttg attgctattc aggcaagccg
    10141 gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc gatagatttg aaccttgatt
    10201 ttcctcggtg gtaatgtgtt catccatcat cgtttctgag caagaaatca cctcgcgact
    10261 gaaagcaaca gtcgcatcac aggttgctga ttcagccgga ttaaacagcc gtaaaatcac
    10321 ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg catcccactg ggggcatttt
    10381 caacaaacta taactttccg gcacgttgaa tccggctttg ttgagcttca tcgcatccca
    10441 tgggattttg ttgtaacact gtactggagt cagccatgct cgcgcctgct gagctacacc
    10501 agcggcggtt ggcgtaccgg tataactcaa taaacttagg cgacaagaaa gcagaccacg
    10561 tagttgtgag tccgggactg gcattttaat tcccgaaggc cgcctaggcc ttaaaagcag
    10621 atcttctttc cccagtaagc ccacgccgcg cagcaacgta atggcaaagg ttttcttctc
    10681 ttcaccgatg acttcaaatt cacgtagccc ttcgctaaag acagccatgc cgttacgccc
    10741 ttcctgtaag gctgcatagt tgagcatatt ccataccgga accggcgctt ctttccagct
    10801 ttcttgctgc cagttgttca ttgcactgtc gttcacgggg cgcgttagcg aaccaaactg
    10861 ggtatctgcc agaacactat cggtgttaaa agatgtaggg atcaggacgc gaaggcgatg
    10921 atcgtcagcc tggttatcaa ggttgatatc cacatcaatg cgcctgttgt tatgactaag
    10981 agtgacaacc aactcgacat ctactctgcc agtggattgt cttgcgctgc gttgttgcag
    11041 attacgcggc accgccattt catagcggat aacagccctg ctctgccagg cctcatgaat
    11101 aatctcgcat tgcggtttcg cattcgccga agtaattacc cactcttctt ttgcgggtga
    11161 atagtcatat tcatcaccat catcagagct ttcttcaatt tgcaacaccc ggtcatagcg
    11221 cacaccgcta tctttatcta ccagttgtag cgttccatcc tcattgagcg caatttgcca
    11281 gaaagcattt tccagtatcc cttcagcgtc actttttgcc gcaatcacgt tgccaggctg
    11341 attcgcttcg atataaagcg tgcgatagcc catagaaggg acaatctggt tgatctgtat
    11401 atcaaactcc ataaagggat cgtaattacc gtaatgaact atttgccgat cgattaggcc
    11461 tggatcgatc tcacgggcat ggcgaataaa atacggtaca ggctgaccgc gatcgtctcg
    11521 tagattaaac tggctggcgc gcagccgcac agtggtgttg atcacttctt cccgcggcca
    11581 gggcatcagg ttaaacagga cgagtttgtc ggcgtcgctc tgcggcatgt tgtcggcgat
    11641 tttacgcata tagaaacgca gcagattatc cgccatgtct tcagccagtt cgaagcgggc
    11701 gacgatttcg cgatgaactt tgtcacgaca gcagcagccg atgctgtcgt gggcatgatt
    11761 ttttaagatc tctttccaca ttttctccag caagccgtgg tggtatccaa aacccaacgt
    11821 cctggccagc gttgccagcg gttccagcag attaacaatc ttattttcaa tacgtgcgtg
    11881 ggcaactttg atatccatgc gcgtggaacc gatggtgcga tgcacgcgca tatatttgcc
    11941 atcaataaat tcccctttca gggttgccag attatctcgc tgcgcttcga tcttctcaaa
    12001 tacctcttca aagcggctca tcacaaactt tcgttgagga taaatttcgc gcagtttctc
    12061 catcacttcg aagatatttt gctgcaatgg catctgatca tgcccattcg gcagcaaaat
    12121 ctctttggtt acagacgctt tttccagcac gtcaaaataa ctgtcgaggc gtttgcgtaa
    12181 tccgttttcg tccgcaggta agtacttacc gatggcataa cccaacggca tcacctgcgc
    12241 cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg gttttatccg taccgtggcg
    12301 ctcagaacat ccacgccaga acatggtgca ggtaatgcca aatccattgt agatatgcgg
    12361 cagttgcccg gacatgccaa aggaatcggg taaataacct attttcatcg gctcgccaaa
    12421 cgcgaggcag tcacgcattc cgtacatcag attacggacg atggattccg cagaaacaat
    12481 cgtggtatcg gtctgggtat accaggggcc gataatcaac ttgccggctt ctaccagttt
    12541 cttcaagcgg tctttgtttt ccggtttcac cgcgaaataa tcttcgagga tcgccgtttg
    12601 cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc agtcggcaca ggatctcttc
    12661 catattattg accagcagaa tgcgtgactc ttcggtggtg aaataccact cccgatccca
    12721 gtgcatatgc ggggtgatgt gaacgcgaga taccgctttc atcgtcgttt cctttttgtt
    12781 tatggcatta cgccatcagt cagatagttg ccatgcttaa ccgcgtgacg ccgccagatc
    12841 aggagaattg cagtcgagat tgcagccccc accaatgccg caccaaacca gccaattgcc
    12901 gccataacac cgcccgcgcc attatcatga agtaaaaaga gcgagaaaat gccagcaccg
    12961 ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc ccgttaccat agagcccagc
    13021 acaaacgaac caataacccg cagcggatct tcaatcgcca tcgggatcgc cccttcggta
    13081 atacctgcca gccctaacag ccaggtggat ttcccggtct caatttcaaa ctctttaaac
    13141 aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg ccgaaaccat tttgacggag
    13201 gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg cgcccaggca gaatgcataa
    13261 gcggctttat tcactggccc tccaaggtca aaggaacaca taaaaccgag aatggcaccc
    13321 agcaacagcg cgttacttcc tgacagaccg ttcagccagg cggtaagcga gttattgatc
    13381 caggcgacag gttcccccac cacaaacagc atcagactgc ccgctcccaa tgtaccgagc
    13441 accgggtaga gataaaaagt caggaagcca ttgaatttac tgctaagacg caagtgattt
    13501 ttcatccagc gtatcaagta accggctatc aatccgccaa cgaccgcgcc gagaaacccg
    13561 gagccgatca tgttggcggc aagtccagcc gcaaagcctg gcgctaacgc cggtttatct
    13621 gccagcgaat aggcggtata ggctgccagc accggtacca tcaaaattcc gagcagcccg
    13681 ccgcccagtt tgcggtacat ccacagccag gagttttctt cattaaacag atcttgtagc
    13741 ccgaagattt gcgacagtaa taccgcgacc gccagcaccg tcccccccgc gacaatcaac
    13801 ggtacggcaa aagagatccc gctcaacagt gcctgtttca gctccgtttt gacgcttttc
    13861 accggttgcg tatcttgctg taaggtacgc gtctcatcgc cacgctcgag ggtaagcgat
    13921 tgttggatca acgcttctgc atggcgaatc ggctcggcaa caggcacaga aagcgcagga
    13981 atgccgttaa aacgctcact ctccttgatg gcgacttcag ccgcaaaaat acaggcagtt
    14041 gcactattga gttgatccgc cgttaaacgc ccttcaatac cgttagcacc ttgcttttca
    14101 acgtagacat tcacgccgag tttgcgtccg gctttttcca gatactccgc agccatgtag
    14161 gtgtgagcaa tacccgccgg gcaggccgtt acgcaaacga tagttggcgc gttggaaaaa
    14221 gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg agagcaattc atcaggcgtc
    14281 gtcgccgact gtatacgcgc ccgaatctca tcatccgcca ggcgcgtggt cagcgctgtc
    14341 agcagttgca tgtgcgtagt acccgcttca ttgaggggaa tcgccagcaa caccactaaa
    14401 tcaaccgctt ccgggccatc cacgccttcc cactgaagcg gctcgctgag tgtggcgacc
    14461 gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg gcacagccaa cccttcactt
    14521 aaggcggtcg ggccaaggct ttcacggcga tacacttctt tcagaaattg ctcagtactg
    14581 gaaattttcc ccagagcagc aagacgttga gtcaacgcgt gaatggcctc ttcacggctg
    14641 gtaaagcgcg cattcagaca caacgcatcg cggtgggtca gagtcgtcag gttcatcatg
    14701 atcctcactt gatcgttttt atagtcgcgc cagtgtgccc gacaaaacaa taccattgcg
    14761 taatcaattt cacttgattg aaattatttg cattttattt gtattgttta caggatgagt
    14821 ttatcgggca taatggctaa attcgtatta atgagatgct aaataatggg acacaaaccc
    14881 ttataccgac agattgctga tcgcattcgt gagcaaattg cgcgtggcga gcttaaacct
    14941 ggcgatgcgt tacctaccga atcggcatta caaacagagt ttggcgtcag ccgggttacg
    15001 gtgcgtcagg ctttgcgcca gttagtcgag caacagatcc tcgaaagcat tcagggcagc
    15061 gggacttacg tcaaagaaga gcgggtcaat tacgatattt ttcagttaac cagttttgat
    15121 gaaaaactgt cggaccgtca cgtcgataca cacagtgaag ttctgatatt tgaagtgatt
    15181 ccggctgacg attttcttca gcaacagcta caaatcaccg tgcaggatcg tgtttggcat
    15241 gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc tggaagaaac ctggatgccg
    15301 cttgcgttgt tcccggatct cacctggcag gtcatggaaa attcgaaata tcactttatt
    15361 gaagaagtga agaagatggt tatcgatcgt agcgaacagg aaatcattcc tctgatgcca
    15421 accgaagaga tgagccgtct gcttaatatc agccagacaa agccgattct tgaaaaagta
    15481 tcgcgtggat atttagttga tggtcgggtg tttgaatata gccgcaacgc ttttaatacc
    15541 gatgactata aatttaccct gatagcgcag cgaaaatcat cgcgataagc acaaaaaggc
    15601 catcatacga tggccaacca tgtcgaaacc gacagccctt cgccattcct gtagagggaa
    15661 gcggcgaggg ctatttctta ttacagatat ttatttcaga acagttttca gtgcttcacc
    15721 gatatccgcc aggctgcgaa cggttttcac gcctgcggct tccagagcag cgaatttctc
    15781 atccgcagtc cctttcccac cggcaatgat cgcacccgcg tggcccatac gtttgccttt
    15841 cggcgcagtc acaccagcga tgtaacccac aactggcttg gtaacatgct ctttgatgta
    15901 cgctgccgct tcttcttcag cgctaccgcc gatctcaccg atcatcacga tcgcttcggt
    15961 ctgcggatct ttttcgaaca tttcgagaat gtcgataaag ttagagccag ggatcgggtc
    16021 accgccgata ccgacacagg tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc
    16081 ttcataggtc agtgtaccgg aacgggaaac gatacccact ttacccggtt tgtgaatgtg
    16141 acccggctgg ataccaattt tgcattcacc cggggtgata acgcctgggc agttcgggcc
    16201 gatcatacga acgcctgctt cgtccagttt cactttcacg gtcagcatat ccagcgtcgg
    16261 gatgccttca gtgatggtga taatcagttt gatgcctgca tcgatagctt ccagaatgga
    16321 gtctttgcag aacggtgctg gtacgtaaat aacagacgcg gaagcgccag tagcagcaac
    16381 agcttcacgc acggtgttga acaccggcag gccgaggtgg gtagtgccgc ctttacctgg
    16441 agttacgcca ccaaccattt tagtgccgta tgcaatggcc tgttctgagt ggaaagtccc
    16501 ctggctaccg gtaaagccct ggcagataac cttggtgttt ttatcaatta aaatggacat
    16561 tatttcccct ccactgcggc aacaacctgc tgagctgcat ccgtcagacc ttttgctgca
    16621 ataatattca ggccgctgtc agccagtttc ttcgcgccga gttcggcgtt gttaccttcc
    16681 agacgtacca cgaccggtac gttaacaccc acttccgcta ccgcgccgat gataccgtca
    16741 gcgatcaggt cgcaacgaac gataccgccg aagatgttaa ccagaacggc tttcactttg
    16801 tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt ctttggttgc gccgccgcca
    16861 acgtccagga agttagccgg ttcgccgccg tgcagtttaa ctatgtccat cgtacccatc
    16921 gccagacctg cgccgttaac catacaaccg atgttaccgt ccagcgcaac gtagttcagt
    16981 tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg actggtcacg catttcgcgc
    17041 agatcaggct ggcggaacag ggcgttgcca tcagcgccca gtttgccgtc gaggcaaatc
    17101 agatcgccct gtttggtgat gaccagcggg ttgatttcga tcagcgccag gtcgcgctcc
    17161 aggaaaatgg tcgccaggcc catgaagatt ttggtgaact gctgaaccag tttaccttcc
    17221 agacccagtt tgaacgccag ctcgcgtccc tgatacggca tcgggccagt cagcggatca
    17281 agcgcaactt tatggatcag gtgcggagtt tcttccgcca ctttttcgat ttccacgccg
    17341 ccttcggtgg aggccataaa gaccacgcga cgggaactac ggtctacaac ggcaccgaga
    17401 tacagctctt tagcgatatc ggtcgccgct tcaaccagaa tctggttaac cggttggcca
    17461 ttggcatctg tttgatacgt taccagacgc ttgcccagcc agttttctgc aaaagcacgg
    17521 atgtcttctt tgctgtttac aactttcaca ccgcccgctt taccgcggcc accagcgtga
    17581 acctgacatt tcactaccca cggaccggca ccgatttttg aagcggcttc ttctgcttcg
    17641 cgcggagtag tacaggcata acccaccggt gctggtaagc catagcgggc aaacagttgt
    17701 tttgcctgat attcatgtaa gttcatgtgt tctgtccatc cttcagtaat cgttatcttt
    17761 taaaccgtag accgacaccc tatccacgtc ggtctacagt gcaggtgaag cttaaactac
    17821 tacacgtcca gcagcagacg cgtcggctct tccagtaact ctttgatcgt taccaggaag
    17881 cccacggatt cgcgaccatc gatcagacgg tgatcgtagg acagcgccag gtacatcatc
    17941 ggcaggatct caacctgacc attcaccgcc atcggacgat ctttgatagc gtgcataccc
    18001 agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca tcagggaacc gaacacacca
    18061 ccgttggtga tggtgaagtt accaccggtc agatcttcaa ccgtcagctt gccatcacgg
    18121 cctttgactg ccagctcttt gattttcttc tcgatgtctg ccatgccgag ggtatcgaca
    18181 tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa ccgccatgct gacgtcgaaa
    18241 tagttgtggt aaaccacgtc atcgccgtcg atagacgcgt tcacttccgg gtaacgtttc
    18301 agggcttcaa ccaccgcttt cacgtagaag gacataaagc ccagacggat gccgtggcgt
    18361 ttttcaaacg cttcaccgta ctgcttacgc agatccataa tcggcttcat gttgacttcg
    18421 ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca gcagacgctc tgccacacgc
    18481 ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg cagccagagc cggttgcgcc
    18541 gccggagcag ccgctgccgg agcagactct ttcgccgggg ctttcgccag atgtttttcc
    18601 acatcttcac gagtcagacg accacccaca ccggtgcctt taatggcgct ggcgtcgaga
    18661 ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg catcgttgtt ttgctcttcc
    18721 agagacgcct gctggcgttg cgccggagtg gacgctttct cttcagattt ggcgctggtt
    18781 tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga tctgacgaga cgttaccgtt
    18841 gtaccttcat cttccagaac cgcatccaga atgccgtctg ctgatgccgg tacttccagt
    18901 accactttgt cagtttcgat ttctaccagc acttcatcac gtacgactgc gtcgccgggt
    18961 tttttatgcc aggttgcgac ggtggcatcg gctacggatt caggcaggtc agggaccaga
    19021 atatctacgc tactcattgt gtatccttta tttattcgac gttcagcgcg tcattaacca
    19081 gatcttgttg ctgtttctgg tgaacggaca tataccctac cgccggagag gcggaggccg
    19141 ggcggcctgc ataacgcaga gaagccccaa acggaatcac ttcacggaaa tgatgctggc
    19201 tgcagtacca tgcgccctgg ttgagcggct cttcctggca ccagacaaaa tccttgacgt
    19261 gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg gaacgggtag agttgctcga
    19321 tacgcacaat ggcgacatcg tgttgattgt tcttacgacg ctgttccagc aggtcgtaat
    19381 aaaccttacc agaacacatc actacgcgct tcacgccctt cggatcaagc tcgtcgattt
    19441 caccgatggc tggcaggaag gtgccgttcg ccagttcttc gaggctggaa accgccagcg
    19501 gatgacgcag cagggatttc ggcgacatca cgaccagcgg acgacgcatc ccgcgcagcg
    19561 cctgacgacg cagcatgtgg taaacctgtg ccggggtaga cggtacgcaa acctgcatgt
    19621 tttgctcagc acaaagttgc agataacgtt ccagacgcgc ggaggagtgc tccggcccct
    19681 gcccttcgta accgtgcggc agcaacatca ccagaccaca catccggccc catttctgct
    19741 cgccagagga gatgaactgg tcgataacca cctgcgcacc gttggcgaag tcaccgaact
    19801 gcgcttccca gatggtcagg gtgcgtggtt ctgcggtggc ataaccatat tcaaacgcca
    19861 gcacggcttc ttctgacagt acggagtccc agacacggaa cgcgccctga ccgttatgga
    19921 tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt gtggatcacc gcgtggcggt
    19981 ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg aaccggaatg ccttcatcaa
    20041 ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc gaacagtttc tcaccggctg
    20101 ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga ctgcatttca actgcttctg
    20161 gtaccgtact gatacgttta gccagttcct gcaggcgctt catctcaact ttgttcgggt
    20221 actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg
    20281 gacgccactc tgccactacg caatcgccag catccagcgc atcgcggtac aggttaacca
    20341 tctcggtggc atcttccagc gtcgccactt tttcctgctc cagcttgtca gcgtagattt
    20401 tgcgcggtgt cggatgtttt ttgattttct gatacatcag tggctgggtt gcgctcggct
    20461 cgtcggcttc gttgtggccg tgacggcggt agcataccag gtcgatgaag acatcacgtt
    20521 taaaggtgtt acggaaatcg agcgccagac gggtcacaaa ggcaacggct tccggatcat
    20581 ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc gatatcagta cagtacggtg
    20641 tagaacgggc atccagcggg ttagaggtgg tgaaaccaac ctggttgttg ataacgatac
    20701 gtaccgtacc gccaacttca taaccacgcg ctttcgacat gttcagagtt tcctgaacca
    20761 cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat tggcagcact ttgttgctgt
    20821 tcggctcatc aagtctgtcc agacgggcac gaacagagcc gataacgacc gggcttacaa
    20881 tctcaaggtg agacgggtta aacgccagcg ccaggtgcac caggccgcca tcggtctgga
    20941 agtcagacga gaagcccatg tggtatttca cgtcgcctgt gccgaggtgt tctttatgtt
    21001 taccggcgaa ctcgtcgaac aagtcttgcg gttttttacc cagcacgttc accagcacgt
    21061 tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg ggtgccgctg ttgccagcgt
    21121 ggcggatcat ctctttaagc atcgggatta acgcgtcacc gccttccagc gagaagcgtt
    21181 ttgcgccagg gaattttgcg ccgaggtaac gctcaaggcc ttcagcggcg gtcagttcgc
    21241 ttaagaagcg ttttttctct tcgctattga aagtcgcgcg accagactcg atacgctgtt
    21301 ggatccagcg tttttcttcg gtgctggtga tgtgcatata ctcggcacca atcgggccgc
    21361 agtaggtttg cttgagggcg gaaataagct cgccgagttt catggtttct ttgccgctgg
    21421 caaatgaacc gacgttgaag gtttcctgga agtctgcttc ggtcagatcg tggaaagacg
    21481 gatccagatc ggccacttta tcttgctgcc acagtcccag cggatctaga ttcgcatgct
    21541 ggtgaccacg gaagcggtat gcgttaatga gctgcaggac tttaacctgc ttcacattgg
    21601 tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat
    21661 attcacgcgt ttgagagtgg aattgatccg gtttgactcc cgtaccaggt aactgctgga
    21721 acgtcgaacg ccagttagcg tcaaccgagt caggatcggt taagaagtct tcatagagct
    21781 gttctatcca gctctggttt gcgccagaga ggtaagaaga gtccaaccag gctttcaaag
    21841 cgctgttctg catcgtgatc ccttaagcat cttttttatg cttacttcgc cgtggatact
    21901 accacgcaca gtgatgtgcg tgccggggtt cacttgcgag ctcttgtcta cgtagtggct
    21961 cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca gtttttaaag atttcctgca
    22021 tttctttgtt acgcctgatg cgctgcgctt atcaggcctg cgggtaatga cttgtaggcc
    22081 ggataaggcg ttcacgccgc atccggcact ggttgcctga tgcaacgctt gcgcgtctta
    22141 tcaggcctac agtttacgca ttacgttgca acaacatcga cttgatatgg ccgatggcgc
    22201 gcgtcgggtt cagccccttc ggacatacac tgacgcagtt catgatgctg tgacagcgga
    22261 atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta tcacggctat
    22321 cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc gataaactta tcgggattcc
    22381 accagaaaga cggacaagag gttgaacaac atgcgcagag aatacattca tacagcccgt
    22441 cgagtttttc gcgctgctct ggcatctgta aatgctcgcg agctggcgga ttttgtccat
    22501 tattcaacag gtaaggctta attttctcat attgcgcata gaattgtccc atgtctacca
    22561 ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat cacaatcttc ttgcccggct
    22621 ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt cttgccgttc atgttcagac
    22681 cgtcggaacc gcacacacct tcacggcagg agcggcggaa cgacaggctg ggatctttct
    22741 ctttcagctg gataagcgca tccagcagca tcatgtcgcg accttcttcc gcttccaggg
    22801 tgtaatcctg catacgcgga gcatcatcaa catccgggtt atagcgataa attgaaaact
    22861 cgagtctcat tttcctgtct ccgcattagt aagtacgaat cttcggcggg aatgccgggc
    22921 gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga ttccgactca ggcagataca
    22981 gggagtggca cagccagttt tcatcatcac gatccgggaa gtcgaagcgg ctatgtgcgc
    23041 cacggctttc ggtacggaag ttggcagaaa cagccgttgc atacgccgtt tccatcaggt
    23101 tatccagttc caggcactca acgcgctggg tgttgaactc gctggaagtg tcatccagac
    23161 gggcattttt cagacgctcg cggatcactt tcaactgctc aagccctttc gccatcgcat
    23221 caccttcacg gaagaccgag aagttatgct gcatacattc ttgcagcgct ttacggatcg
    23281 ccaccggatc ttcaccgtta cgattattgt tccaccggtt caggcgatcc agagacgctt
    23341 caacatcaga ctcgctggca tcgtgcagtg cgccctgctc ggcgatagac tcttgcagat
    23401 gcagacctgc cgcgcgacca aagaccacca ggtccagcag cgagttgccg cccagacggt
    23461 tagcgccgtg taccgataca caagcgattt caccaacggc aaacagtccc ggaacaacca
    23521 catcttcgcc tttctcattc acagtcagcg cctgaccggt aactttggtc ggaataccgc
    23581 ccatcatgta gtgacaggtt gggataaccg gaatcggctc tttcaccgga tcgacgtgag
    23641 cgaaggtacg ggaaagctcc aggatacccg gcagacggga ttcgagaact tctttaccca
    23701 ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc atcacagccg cgaccttcac
    23761 ggatttcaat catgatggaa cgcgccacca catcacggcc cgccaggtct ttggcgttcg
    23821 gcgcataacg ctccataaag cgttcgccat gtttgttcag cagataaccg ccttcaccac
    23881 ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc ggtcgggtgg aattgccaca
    23941 tttccatatc ctgcaccggt acgcctgcgc ggatagccat gccgacaccg tcgccagtgt
    24001 taatgtgggc gttagtggtg gactgataaa tacgccctgc tccgccagtc gccagcacgg
    24061 tagcgcgggc tttgaaataa accacttcac cggtttcgat gcacagtgcg gtacaaccca
    24121 ccaccgcgcc atcctggttt ttcaccagat ccagcgcata ccactcggag aaaatggtgg
    24181 tgtggttttt caggttctgc tgataaagcg tgtgcaacag tgcgtgaccg gtacggtcag
    24241 cagccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga ccgccaaacg
    24301 gacgttgata gatacgacca tcatcgagac gcgagaacgg caggcccatg tgttcgagtt
    24361 ccagaatcgc ttccggcccg gttttacaca tatattcaat cgcgtcctgg tcaccgatat
    24421 agtccgaccc ttttacggtg tcgtacatat gccattccca gttatcttca tgggtattac
    24481 ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc gggaagactt
    24541 tagagagcag ggcacaggtc tggccgctct gggaaatttg cagcgccgcg cgcatacctg
    24601 cgccaccggc accaatcaca actgcatcaa attctctgac tggcaatttc atcacacacc
    24661 ccacaccaca acgaatccat aaatcacgta aaccaccagt gcaacgacaa tcaccagttg
    24721 cagcatcagg cgcaaggcca gcggtttaac gtagtcggtc aacacctgcc acatgccgat
    24781 ccaggcatga atcaagatag aaaacagcgc cagcagggtg aacactttgg tgaacgcaga
    24841 ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca ctggtggcga aaaaaccgac
    24901 catataaatg atgtagagcg tcaggacgat agcggtagcg cgaacgagga tgaaatcatg
    24961 tacgccattg cgtcctaatg cggaggcgtt gcttaccata cgaggactcc tgcgagaagt
    25021 gaaagcacga cagtaataac aaaggagatt ttggcggagc gtttacccgc ttcgaatgtt
    25081 tcttccagat agccaaaatc catcatcatg tggcgaatac ctacgacgac gtgatacgcc
    25141 agagcggtaa ggatgcccca catgataaat ttgacgaaga agctgcccat aatcgcggaa
    25201 gcttgctcga aaccttcagg ggaagagagg ctggtaccca gaagccactg caggatgccc
    25261 actgcaacaa aggtgatcac accggaaacg cgatggagaa tggacgctat cgccgtgatg
    25321 gggaaccgga tggtctgtag gtccagatta acaggtcttt gttttttcac atttcttatc
    25381 atgaataacg cccacatgct gttcttatta ttccctgggg actacgggca cagaggttaa
    25441 ctttctgtta cctggagacg tcgggatttc cttcctccgg tctgcttgcg ggtcagacag
    25501 cgtcctttcc ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa aacgacacgt
    25561 tacaacgctg ggtggctcgg gattgcaggg tgttccggag acctggcggc agtataggct
    25621 gttcacaaaa tcattacaat taacctacat atagtttgtc gggttttatc ctgaacagtg
    25681 atccaggtca cgataacaac atttatttaa tttttaatca tctaatttga caatcattca
    25741 acaaagttgt tacaaacatt accaggaaaa gcatataatg cgtaaaagtt atgaagtcgg
    25801 tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac
    25861 agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta
    25921 ttcgtcatca atggattctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc
    25981 tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc
    26041 aaatttaagt tccggcagtc ttacgcaata aggcgctaag gagaccttaa atggctgata
    26101 caaaagcaaa actcaacctc aacggggaca cagctgttga actggatgtg ctgaaaggca
    26161 cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg
    26221 acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg
    26281 aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc
    26341 tagaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat
    26401 ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccacg
    26461 ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg
    26521 cgttctatca cgactcgctg gatgttaaca atcctcgtca tcgtgaaatt gccgcgttcc
    26581 gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc
    26641 catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct
    26701 ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc
    26761 tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct
    26821 cttcgggggc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg
    26881 cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac
    26941 acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct
    27001 tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc
    27061 atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg
    27121 aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt
    27181 tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt
    27241 tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac agttacggta
    27301 tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg
    27361 atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg
    27421 cgggttaatt tttatatgcc taaacacaaa aaattgtaaa aataaaatcc actatcggac
    27481 attcatatat tctaaaaaaa aatacatcgg tataaattat aaacaaatag atgattttta
    27541 atcatctatc ttcctctcag ttacatgaat aaaccaccaa atatagcgca cccctataat
    27601 tcccaatact ttattcatca aacatttaat tcataccatc aattaattac ttttacattt
    27661 aataaaatta ataatatttt taagaaaaac taaaaacatt cgacaatcaa ataagatatt
    27721 cttgcaccga tatacttttt tcttttctgt taaaggactc agttatgttt aaaggacaaa
    27781 aaacattggc cgcactggcc gtatctctgc tgttcacagc acctgtttat gcggctgatg
    27841 aaggatccgg tgaaattcac tttaaaggtg aagttattga agcaccgtgt gaaatacatc
    27901 aggatgatat tgataaagag gttgaactcg gtcaggtgac caccagccac attaatcagt
    27961 cacatcacag cgatgccgtt gctgtcgacc tgctcttagt caactgtgat ctggaaaact
    28021 ccagcaacgg ttccggtggc aagatttcta aagttgcagt cacctttgat agctcagcga
    28081 aaaccaccgg cgcagatccg attctcaaca acaccagcac gggtgaagcc accggcgtcg
    28141 gcgtacgttt aatgaataaa gatcaaagca atatcgtttt aggcaccgct actccagata
    28201 tcgacctggc tccgacctcc agcgaacaaa cgctgaattt ctttgcctgg atggaacaaa
    28261 ttgatcaggc tacacctgta acgccaggcg cagttacggc gaatgcaacc tacgtgctgg
    28321 attataaata agtattactt tggcggcgct atcgccgcct ttcataaaat atttatcagg
    28381 tatggacacc gtgaatattt atcgactctc ttttgtatcc tgcctggtcg tggcgatgcc
    28441 ttgcgcattg gcggtcgaat tcaatctgaa tgttctcgat aaatcaatgc gcgaccgcat
    28501 tgatatttca ttattaaagg aaaaaggagt cattgctccc ggtgagtatt ttgttagcgt
    28561 tgcggtaaat aataaccaaa tcagtaacga gcaaaagatt aactggcaca aaaatgacga
    28621 taaaaccatt ccgtgcatca atgatttact ggtcgataaa tttggcttaa aacctgaagt
    28681 ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt agctcccgac ctgaaatgct
    28741 cttcaatttc gatcaagcca atcagcaact gaatattagt attccgcaag cctggctggc
    28801 gtggcactca gaaaactgga ccccaccctc cacatggaaa gaaggtgtcg ccggtgtcct
    28861 gatggattac aacttgtttg ccagcagcta ccgcccacag gatggcagca gcagcactaa
    28921 tcttaacgcc tatggaacag ccggaatcaa cacgggggca tggcgcttac gcagtgatta
    28981 tcagttgaat catactgata gcgatgataa ccatgaacag tcaggcgaaa tatcacgtac
    29041 ctatcttttt cgcccgttac cgcaattagg ttcaaaatta accctcggcg aaacggattt
    29101 tagtcccaat attttcgacg gtttttctta taccggcgcg gcactggcaa gtgacgatcg
    29161 aatgttgcca tgggaactac gcggctacgc cccacaaatt agcggtattg cacagaccaa
    29221 tgccacggtg acgatcagtc aatcaggccg cgtcatttac cagaaaaaag tcccaccagg
    29281 cccatttatc attgacgacc ttaatcagtc tgttcagggc acactggatg tcaaagtgac
    29341 ggaagaagat ggtcgggtga acaatttcca ggtttcagca gcatcgacgc ccttcctgac
    29401 tcgtcaggga caggttcgct ataaactggc cgcgggtcag ccacggccct ccatgtcaca
    29461 tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc tgggggatgc tgtcaaacac
    29521 ctcgctgtac agcggcctgc tgctttctgg tgatgactac cattctgccg caatgggtat
    29581 tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat gtcacgtggg ccagtagcca
    29641 ttttgatact cagcaggacg agcggggctt aagctaccgt tttaattaca gcaaacaagt
    29701 ggatgccact aacagcacga tttccctcgc cgcttatcgt ttctccgatc gtcattttca
    29761 cagctacgcc aactatctgg atcacaaata caacgacagc gatgcgcagg acgaaaaaca
    29821 gacgatcagc ttatctgtgg gccaaccgat taccccacta aacctcaatc tttacgccaa
    29881 cctgctacat caaacctggt ggaatgcaga cgcctccacg accgccaaca tcacggcagg
    29941 ttttaatgtt gatattggtg actggagaga tatctctatt tctacgtcat tcaatacaac
    30001 ccattacgaa gataaagatc gcgacaacca gatttacctg tcgatttcgc tccccttcgg
    30061 taacggtggt cgggttggct atgacatgca aaacagtagc cacagcacca cacaccgcat
    30121 gtcgtggaac gatacgctgg atgaacgtaa tagctggggc atgtctgccg gactgcaatc
    30181 cgaccgtcct gacaatggag cccaggtgag cggtaactat cagcacctga gttcagcggg
    30241 tgagtgggat atttctggta cctatgccgc caatgattac agttccgtca gcagcagctg
    30301 gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt catcgccgca gctccaccaa
    30361 tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat attccggttc agggcaatct
    30421 cgactacacc aaccattttg gcattgcggt ggtgccgttg atttccagtt accagccttc
    30481 caccgtggcg gtgaacatga atgacttacc cgacggcgta acagttgcag aaaacgttat
    30541 caaagaaacg tggattgaag gcgcgatagg ttacaaatca ctggcttccc gttccggtaa
    30601 agacgttaac gtcatcattc gcaacgccag cggtcagttt cctcccctcg gagcggatat
    30661 ccgccaggat gacagcggca ttagcgtggg gatggttggc gaggaaggac atgcctggtt
    30721 aagcggagtc gctgaaaatc aaaagtttac cgtggtctgg ggtgatagcc agcattgctc
    30781 gctccatctt cctgaacata tggaagacac cgcaaatcgc ctgattttac cttgtcatta
    30841 attaaggaaa tgaaaatgac atttatgaaa ggactacccc ttttgttgtt ggttgccagt
    30901 ctgtgcagcc acgctgcact acaacccgat cgcactcgta ttgtgtttaa cgctaatgat
    30961 aaagctacca gtctgcgggt ggataatcgt agcgataaac tcccctacct cgcttattcc
    31021 tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc tggtcgccct gccgccgata
    31081 caacgtctgg aaccgaaagc aacaacacag gtacggatag taaagcaagc ctcgaccaca
    31141 aaattaccgg gcgatcgcga aacgctattt ttctacaata tgcgtgaaat tcctcccgcc
    31201 ccggaaaaaa acagcgacca tgccgttctg caagtcgcca ttcagagtcg tatcaaggtg
    31261 ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa aggtcgaact gcagttacag
    31321 gtcagccagc agggcaacca actgacactg aaaaatccta ccgcgtatta tctgaccatt
    31381 gcctacctgg gacgaaatga aaaaggcgtt ctccccggct tcaaaaccgt gatggttgcg
    31441 ccttttagta cggtcaacac aaacaccgga aattatagcg gcagccagtt ttatctcggt
    31501 tatatggatg attacggcgc gttacgcatg acgacgctca actgcagcgg acaatgccat
    31561 ttacaagccg tggaggcgaa gaaatgagtg ctggcaaggg agtgttgctc gtcatctgtc
    31621 tgttatttct gtcgttgaag tctgcgcttg ccctgaactg ctactttggt tcatccggtg
    31681 gctcggtaga aaaatcagaa gcgattcaac cttttgctgt accaggcaat gccaaactcg
    31741 gcgataagat ctgggaatct gacgatatta aaataagatc tgggaatctg acgatattaa
    31801 aattcccgtc tattgtgaca acaataccaa cggcaacttt gaaagcgaac atgtctacgt
    31861 atgggtaaac ccctacccgg gggtgcagga tcgttattat caactcggag taacttacaa
    31921 tggcgttgat tatgacgcca accagggaaa aagccgcatc gacactaacc agtgtatcga
    31981 cagtaagaac attgacattt atacccctga tgggatggca gaacaaaatt tgctcgggtg
    32041 atcccgctaa tattcacatg tcgcgaacct ttctcgctcg catgcggtta tacgtcaaaa
    32101 tacgagaaat gccgccgcat gattatcaaa gtacgcttag cgactatatc gtcgtgcaat
    32161 ttgacggtgc cggtagcgtt aacgaagacc ctactgccca aaacctgaaa tatcatatta
    32221 ctggtctgga aaacattcgc gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa
    32281 cacagatgat tgattttggt aaatttaatt tgctggatat tcgtcgccac acaatgtcga
    32341 aaacattcag tattaagacg acaaaatcac aaaatgacca atgcaccgac ggatttaaag
    32401 tcagttcctc cttttatacc gaagaaacgt tggttgaaga agataaagca ttgttgattg
    32461 gtaatggtct gaagctacgt ttgctggatg aaaacgcctc gccttatacc ttcaataaat
    32521 atgctgaata cgccgatttc accagcgaca tgttggtcta cgaaaaaacc tatacagctg
    32581 aactttcgtc cattgcaggt acccccatcg aggctgggcc cttcgataca gtggtgcttt
    32641 ttaaaattaa ctataactga tttgccatga tgccctttgc agccggaaag ggcatctctt
    32701 tcagaaaatt gctgattttc cccttttcct tgattgctgt tattttttcc tagcccggct
    32761 ttctttatgc tgtatcgata caccaaagag aaaataatga gggagcgaag gatgccagtt
    32821 ttgcagtggg gaatgttatg tgtgctatca cttctccttt ctattggttt cctcgcggta
    32881 catctcccgg cggcgctatt acttgggccg atgatcgccg ggatcatctt cagtatgcgc
    32941 ggaattactc tgcaacttcc ccgctccgct tttctcgccg cgcaggccat tcttggctgc
    33001 atgattgcgc aaaacctcac tggttcgatt ctcaccacgc tggccgccaa ctggccgatc
    33061 gtgcttgcta ttttactggt gacgctgctt tccagcgcca tcgtgggttg gttattggtg
    33121 cgctatagct cactgcctgg aaataccggt gcctggggtt cttctccagg cggcgcagcg
    33181 gcaatggtcg ctatggcgca agattacggc gcagatattc gcctggtggc gtttatgcaa
    33241 tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg tcacccgcat gatgctgggt
    33301 gataacgctg aagcggttaa ccagcaaatc gtctggttcc cgccagtgag cattaatctc
    33361 ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg gatgcatgct gcgtcttcct
    33421 tccggcacga tgctcatccc gatgctggcg ggcgcagtgc tccagtctgg tcagctcatc
    33481 accatcgaac taccggaatg gctgctggcg atggcgtata tggcaattgg ctggcggatt
    33541 ggtcttggtt tcgataaaca aatcttactg cgggcactac gcccgctacc gcaaatcctg
    33601 ctgtcgattt ttgctctgct ggctatttgt gcaggtatgg cgtggggact gacccggttt
    33661 atgcatattg attttatgac tgcctacctc gccaccagtc ccggcgggct tgataccgtg
    33721 gcggtcatcg ccgcagggag caatgccgat atggcgctta ttatggcgat gcaaaccctg
    33781 cgcctgttca gtattttgct gacggggcca gccattgcgc ggtttatttc aacctatgcg
    33841 ccgaagcggt cggcctagtg ctggcagcct ggacaccagt aaaacgggcg cgaggacaac
    33901 gtggttttct caatgatgcc gccacagcgt tcgcacagtt cgccatcacg atgaaaaacc
    33961 ttaaagcgaa acagcgcccc atgatgctta ttctcatcca cctgcccccg cgtagcgtaa
    34021 gaaaaccgag gaatctccag taacgcgtgt gcgagtgcat ccagttgcgc cgcattgaga
    34081 tctttcgctt tatgatttcc agtcaaccca acctgccaga ggatctccac ccgcaaataa
    34141 ttgccaagcc cagccagaaa cgcctgatcg agcagtaatc ctgcaaactg acggttacga
    34201 aagcgcggcg acaataaccg ttcctttacc acctccggcg tcagattcgg atccagcaca
    34261 tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt gttccggcct caacatctca
    34321 atatccgagg cgctataaag cagaatggtt ttgtcagccg tttgcagttt tacccgcaat
    34381 actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc gccagacgcc gtaaagctga
    34441 ttatggctgt agagcgttaa accgttggga aaatgagtta acaacgcctt accacgcgtt
    34501 tccacatggg taacgtgttg accgataagt tgtgattgat aagtttttaa ctgcgggaag
    34561 gcaaaccaga catcagttag tggtttgcct ttgatcgccg cctccaggtt atccgctgca
    34621 cggcggatct ccgggccttc aggcatggtg atatcctttt ttcattcttt attaagcggg
    34681 gttatgctgc gacaacaatc ccctttttgg caaatgtagc tcgtaatcgg cgggcgaacg
    34741 ccagagcgtg ctcgccgtcg ccatgcaggc agaccgtttg cgccgcgacc gttgcccatt
    34801 cgccggtgat acttttgact ctgccgtgtt gcaccatctc cagcgtttgc gccaacgcct
    34861 gttcttcgtt ttcaatcaac gcgcctggct gacttcgcgg caccagcgag ccgtcagcct
    34921 gataaccgcg atcggcaaac acttcctcgc gcgttgtcag gccatattgc ttgcctgcac
    34981 gaatcagctc gcttcctgcc agcccaacga gaaccaatgc cggatcgcaa gcgtataccg
    35041 ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc ctggttgtac aacatgccgt
    35101 gcggtttgac atgacgcatt acgccgcctt gcgcacgggc aatcgttgcc agcgcgccaa
    35161 tttgatacag cgtctgggcg taaacggttt ccggcggcag ctgcatggcg ctgcgaccaa
    35221 aattttccct gtcgggaaaa ctcgggtgag cgccaatcgc gacaccattt tttattgctt
    35281 cacgcacgca agcctgcatg gtttgcgcat cgcctgcatg aaatccacaa gcaatattgg
    35341 cagaggaaac cagcgttaat agctctgcgt cgctggcgca gccttcgccc agatcggcgt
    35401 tcaggtcaat tttcattgtg cagccgccac gctaattgtt caaaataacg ttgctgatct
    35461 tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa aatgaatcgg ctgaccgagc
    35521 ggaatttgcg ccagatggta catatcagcc tcaatgatac aggcaatacg tggataacca
    35581 ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc cgttatgcgg cacctgcacc
    35641 acgcccggta acaaaccgtg agataacagt tcgcgatcgg tgatgcgttt taaaatttgc
    35701 ccctgtaaac gatagcccat tcggttactt tgcgagctaa gctgccaggg cgaacgccag
    35761 aatgcatcct gcgaggcgcg atcgaactca tgatattccg gccccggcaa ggcgcgaatg
    35821 cggttgcccc acagcagctg tttaacgccc tgcgcttcca taaaatcacg cttcgatttg
    35881 ccaatcggga gtcagtcacc atccttcagt aaacggcctt ccagcccgcc aatccccact
    35941 ttgagatcgg tgctgcatga ccccattacc ggcggaacat caataccacc cgcgaccgcc
    36001 agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta aacgctggcc tgctttcatc
    36061 ggcaatcgcc agccggtcca gacggcgtta tcatccaacc gcgcttcgca acctgcaccc
    36121 gtcagggcaa accacccatc agtttcgaac tcaactgtta actgaccgag cgtgatctcc
    36181 agcgcggggg cattggcgtc attacccacc agcaggttag caatgcgcag cgcaggcata
    36241 tccagtgcgc cacagtggct gatacccgac tggcaaaaac cgtgacgacc gccatcctgc
    36301 acagtggtgt acatgcccgc acgaataatc ttcagcatat tccctccttc tgcggtacaa
    36361 atcgcacgct gtctcccgga cgtaataaga tgggttcgtc acgcgccgga tcaaacaggc
    36421 tgagtgaggt atgaccaatc aactgccagc cacccggcgt tgccagcgga taaacaccag
    36481 tctgcggccc gccgatcccg acagaacctg ccggaacgag caagcgcggt tcagcgcgcc
    36541 gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg gaagcccggt tgaaaaccta
    36601 aaaaccagac cacgtattcc acggaggagt gcaattcaac aacctgtttt tcgctcaacc
    36661 cgcaatacgc cgcgaccacc gccaaatccg gtccgcctgc accaccgtaa accaccggaa
    36721 tttcaataaa gcgagactca ggttccaggg cctcgctctc ctcccaccag cgttgcaaac
    36781 gctctatggc atccagcgcc agcgactcag gattacgcaa aatcaccgtg atattgttca
    36841 tgccgggaat ggcttcaacc acattcggca tatccaccag acgctgcgcc agtcgccaga
    36901 tccgtttctg gctagccagc gtcaccggcg gttccagttc cagcactacc gccgtttcac
    36961 ctatcagata acaacgcgct cgttgcactt tcgatccctc tgattattcg ttatgcagga
    37021 ttaggaatat caataaaggt cacatcaaga tcggtatttt cattcagcca ctcgctcaat
    37081 gcgcgaatac caccgcgttc agtggcatgg tgacctgcag cataaaaatg caatccctgc
    37141 tcgcgggctg aatgaatggt ctgttcagaa acttcgccag taataaaagc atccacgcca
    37201 aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg tgcaccaggc gacgcgctgg
    37261 accacctcag gtccggtatc gccacaccat aacggcttac gtcccagacg cgcttcaatc
    37321 caggaggcta gttccattcc cggcacaggc atggtcagct ctccccacgg caccaacggc
    37381 tcaatttcgc ccatgaccgt gatccccagt aacgccgcca gctgtgcgtt attgcccagc
    37441 tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt tgatatcatt cgccagcaac
    37501 gtttttaaac ggttacgctt cattccgcga atgaccggag actctccttt ccagaagtag
    37561 ccatgatgca caatgaccgc atccgcgccc agacgcactg cctcatcgag caaagcctgg
    37621 ctggcggtga caccggtaac aattttttgc accgtctctt tgccttccac ctgcaaacca
    37681 ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt cgttaatcag ttgttccagt
    37741 tcggtgtttt tcatcttcgc gccttccgta ttctgatagc tacaccgtta acatagcgtt
    37801 acatcgcctg ttcgtcgata gccaatcttt tgacatatta agaaacaaac ccgacgccaa
    37861 aagacgcaaa ttgtttatat catcacgctt cagctaatgt atatttatgc aatcagacag
    37921 catattgcat aaataatcag atctgtatca aagttttaac tttaaggaaa cacaagcacg
    37981 ccgcgcaact attacgtgac gttgccgtca attgtggaat tattatgaat aaacacgcat
    38041 cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat ctgggaatat tttagctttt
    38101 acggcatgcg tgccctgctg attctctatc tcaccaatca actaaaatac aacgatactc
    38161 acgcctacga gttatttagc gcctactgtt cgctggtgta tgtcacgcca atcctcggtg
    38221 gctttttggc ggataaagtt ctcggcaatc gcatggcggt gatgctgggg gcgttgttga
    38281 tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca tccgtcattc ctctatctgt
    38341 ccctggcgat tatcgtctgc ggctatggcc tgtttaaatc taacgtaagc tgtctgctcg
    38401 gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg tttctcgctg atgtatgcgg
    38461 cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg ttacgcccag gaagagtaca
    38521 gctgggcgat gggctttggc ctggcggcgg ttggaatgat cgcgggtctg gtcattttct
    38581 tatgtggcaa tcgtcatttc actcataccc gcggcgttaa caaaaaagta ctgcgtgcga
    38641 caaactttct cctgccgaac tggggatggc tgctggttct gctggtggca acgcctgcac
    38701 tgattaccgt gctgttctgg aaagagtggt cggtatacgc cttaattgtc gcgactatta
    38761 ttggcctggg tgtactggca aaaatttatc gcaaagcaga aaaccagaaa cagcggaagg
    38821 agctggggct gattgtgacg ctcaccttct tcagtatgtt gttctgggcc ttcgcacaac
    38881 agggcggtag ctcgattagc ctttatatcg accgcttcgt taaccgcgat atgtttggtt
    38941 ataccgttcc gaccgcgatg ttccagtcga ttaatgcctt cgcagttatg ctgtgcggtg
    39001 tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa tcgtaccgtg cgcatctggg
    39061 gaaaatttgc tcttggcctt ggcctgatga gcgccggatt ctgcattctg accttaagcg
    39121 cccgctggtc cgcaatgtat ggtcactctt ctctgccact gatggtgtta ggcctcgcgg
    39181 tgatgggctt tgcggaactg tttatcgacc cggttgccat gtcgcaaatt acgcgcattg
    39241 aaattccggg cgtgaccggc gtattaaccg gcatctacat gctgctttct ggcgcgatcg
    39301 cgaactatct ggcgggcgtg attgccgatc agacatcgca ggcttcgttt gatgcttccg
    39361 gggcgatcaa ctactccatc aatgcatata ttgaagtatt tgatcaaatt acctggggcg
    39421 cactggcgtg tgtaggactg gtactgatga tttggctgta tcaggcgctg aaattcagaa
    39481 accgcgcgct ggcgctggag tcataatagg ccagcaccga tgcctgatgc gacgcttgac
    39541 gcgtcttatc aggcctacgt atttcctgca atttattgaa tttgcacaaa tttgtaggct
    39601 ggattaggcg ttcacgccgc atccggcatc tgggctcgat tgcctgacgc gtctgttatt
    39661 tccccttccg cgccgcctca tacgctgcca acgtttgtac tctcgcctct ttgtgctcga
    39721 ctatcggttg cggataatcc agcttcacac ctgctttctg cgcccacttc cacggctcat
    39781 gcaccgcttt ccctggtaca tcgcgcagct ccggtaacca tcgacgaata aactcgccct
    39841 cacggtcaaa tttctcgccc tgagttatcg gattgaaaat acgaaaatac ggcgcagcat
    39901 cagtacccgt agaagccgcc cactgccagc caccgttatt agctgccaaa tcaccatcaa
    39961 tcagctgcga catgaaatat cgctcgcctt cgcgccagtc gatcaacaaa tctttcacca
    40021 gaaaactggc tgtaatcatc cgtagccggt tatgcatcca gccagtgctg ttaagctgac
    40081 gcatagcggc atcaataatc gggtatcccg ttttgccttt ctgccaggcc tgtaaatgtg
    40141 cgggattgct ttgccactgc acacgatccg tccaggcaat aaatggacga tgtttacaca
    40201 acgaggggta atacgtcatc agatgacggt agaactcgcg ccagatcagc tcactaagcc
    40261 agacactacc ggccccaccg tccagcgcct gcggctgttc cgccaacaag cgatgcaaac
    40321 actggcgagg cgataacccg cccgttgcca agctggcgga caaacggctg gtgccttcca
    40381 ctgccggaaa atctcgttgt tgctcatact ctccagcacc gttctcgcaa aactggcgta
    40441 attgcgcaat cgccgctttt tcttccaccg ggaaatgcgc agtatcgaaa gactgacgag
    40501 gataattcag cgtaatggat ggcgcgggct ttatcgatcc gctactacga acttttggtg
    40561 cagcgacgca ctccggcatc ccttcccgca gccgtttcag ccaggcattc ttaaaaggcg
    40621 taaagacttt gtacatctcg tgattgccgg tcatcaccgc ccccggcggc aggatcacgc
    40681 tgtcatcaaa tccttcacac accacgttac gcagcgctct ttcaacgtcc acatcccgcg
    40741 ctcgctcatt cacctcatac tgatagttat aaaaaagatg agtaacgctg ttttccgcgc
    40801 acacctgttt aactatttcg acactggcga caaagtcatc cacttcacgg aacaataaag
    40861 gaataccttt ttccgccagc gcgatttgta gcccattcag ttgagcattg ataagttcag
    40921 cctgacgcgg cgacatgtta tgcgtctccc acttgcgtgg tgtagcgata tacagcgcca
    40981 gcacgcgtgc agacgaattg cggcaggcgg cagccagtgc gagattatcg tgcagacgta
    41041 aatcctggcg aaaccaggcc agatgggtag tcataaaact cctgaatggc gcaagttaat
    41101 cacacgcggc caaagttcga aataacgctg ccctgaaagc caggcgtcag gataaagcgc
    41161 catatagtgt ttgatacgca tcagcggcgc aagaagtggt tgctcgccac ggcgataact
    41221 gtcgataagc gcagccagct gctggcgttc tgtggaatca atatgcgggc gaaaataacc
    41281 ctgaacgtgc atcaaaacgt tggtgtgatc gcgcgggttg gcgggatgag aaagcaaaac
    41341 aattacgcgt tggcggtact ggttgtaaaa atcatccaga ttttgccact gatgaatatc
    41401 agccacaaac gggccaagtt tgcggtactc gggctgagaa tgggccagaa agacgagttt
    41461 atagcggctg tgataatcga gaagcgcccc gcgcgttaag ccatgttctt tcagtcgatg
    41521 taactcatga agggcgaaga tacggattag ggtgctgtca tcaaatcgtt gtagattcat
    41581 tactgctcct gaacggtata caggagtaag tgtaggcgtt ggtaaatgat gtttgtgaag
    41641 ttaatccgct agcagggcaa gattacgaaa gcgcgctccc cgcaagcaac taatcaaggt
    41701 agttaatcat ggctgaacac gatattacgc cagaagtcac tatttccaaa acacagcgcc
    41761 gctataaagt cggctatgtc agagcccgcc accaggaccg caaaaccggt atagcccggt
    41821 attattcaca acatcccagt ctgcacctta agggcgactg gctgaaagag gccggatttg
    41881 ataccggacg gggtgtcacg gtgaagattt cggagggggg cctgaccatc atcgctgaca
    41941 gtgacgaggt gcaggaactg cgggatggga tgtttagtgt gctgaacgaa agttaaatat
    42001 taaaaaagct gggaagattt aaaaccttcc tagcttttta ttctgtctta ttataatgga
    42061 tactcatcat cttccgatga atttataaat tgctccaagt ttacatgaag agtacttaac
    42121 ctagttttta actcattaag tgaagcgata atttcatatt ttccaacata atcatatgat
    42181 ggatcgtatt cattatctat gtcagaagct aaaaatgatg atattcgtag tttaaattta
    42241 gcttcatctt tattacattc attggcataa ggtgaatgcc ctatcatagc atattcatct
    42301 aatctaaaag gataagatgg ttttaattca tcgatcaatg aaatttcaat ccatgttcta
    42361 tatccaggca ttaatggact aatttctttg agcaaaacct ttccacttaa taatgtattg
    42421 agattgttca ttattatgtc tcccacggga aaaatatatg tctaacactt ccaccatgtt
    42481 gatgaacttg taaatgtggt aaatcaccat caggatttcc tggcccatga cctgacactc
    42541 tatatgaagg ttttgttaca gaaggatctt ttatatcatc ccaatgatat gaacctgctt
    42601 ttgttttatt ttcagtaaga tattccatta agttcttagt gctggggcct gactcacctg
    42661 tcatattttt ataattatga cctaaatatc tatccataaa caattgttca gcgtcacttc
    42721 tagacctaac agtaactttg gtttcactac tatctaataa acgattcgct ttttggttac
    42781 taacagcaca tggagttaat ccaagtggat cagcatattt tagaggatta ggtgcataac
    42841 tgtagagatt taaaccgcct ctcaacccta tcgggtcagt taccatgaac cgcccgacat
    42901 ccggatcata gtacctgtgc agattgtagt gcagccccgt ttccctgtcg aggtactgtc
    42961 cctggtagcg caggttctgc tccaccccgc cgtggtcctt ctcctgtatc gtgttacccc
    43021 agacctgata ccccgtctcc cacacgattt tcccgtcgct gtccgtcatc tcttccggcg
    43081 cgccgttcac atccgtgtga aaatacagta tccggttcgg gccctctttg cccgccttat
    43141 ccacccgcgc cagcggttca taactcccct ggtccctgta gatgtacagg ctgcctgttc
    43201 cctccggcct gctctcctgc gccagccgca ttccgtccca taaaaatttt gtccggttgt
    43261 acggcttacc gtcagggctg atgtgctgtt tttccgtccg cctccccagt gcatcgtaca
    43321 cataccggta tgtcccccgc gctgtcgtca cttccaccat ccggtgttcc gcatcataac
    43381 ggtaactctg cgttccgctc cggccctcct tttccaccgc acggccaaat tcgtcataac
    43441 ggtagtgcac accacggtaa gacatcagcc ggttgaacgg caccacgctg ttttctgccg
    43501 tggccctttc ccccgcgcgc ctccccagca ggtttcccgc cgcgtccacc tccgtttccg
    43561 gctggtgcca caccgggtgc cactgcgcct gctccacccg ccccagcggg tcgtgcgtct
    43621 ccgtgatatg cccctgcagg tcatacacat agcgccattt gccgccctga gggtcggtca
    43681 tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt cgtcacctgt ccggcttcgt
    43741 cctggtacgc cgtcacctga ttctgcgcat cccactggtg ataacgtgtg cgccccagtc
    43801 cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc cagccggtac tgttcaccgc
    43861 tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc cggaccttca taccagcccc
    43921 actggtaata atacccttcg ccgcccggct ggcggtgcag cgtcatgcag tggttttcgt
    43981 cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta cacgccgttc aggtggccgg
    44041 cctcgtcata gctgtaacga cgcagcagta aaggtgcagt ctcagggaaa tgctgataaa
    44101 tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata cagacggatg cacggacgct
    44161 gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc gtagtacaga ttcagacagt
    44221 tgctgttacg gtcgcccagc attttcagcc gctggcgctg cgggtggtgc gggtcagcct
    44281 caaacagaaa acactgcccg tcatcgctgc tgataagcca gtgcccctgc tcaccacgcc
    44341 ttaccgccag cccttcaccg gggctgtaaa acccgccccc cggcagtacg gcttccatat
    44401 ccaagcgtcg ccccatgccc gacacaaagg tcatgcagtt ttcgtctgcg ctacccggtg
    44461 tgcacacgag gctgacttca tacattacac tccagccctg accaaacatt ccttccgtac
    44521 gttcgtcacg actgctgtac acgcgctgcc agtccagcgg aaagtggccc ggcagactga
    44581 aatcgaagtc atcgtcatcg gccaggtatt tggccccggt ggcggcattg accggatgcg
    44641 gaagggcact ggtggccttc tgcgtcagca tgctcatccc gacactggca gcaaaacagg
    44701 cgattttaga aagcattttg cccgggcgaa gaaacatcag cgccacggtt gccaccaggg
    44761 ttatctgcga cctgccgctt ctgatatccc tgaccaccag taacggaccg ccaatgcgca
    44821 catccggtga cacaaacgcc ccgttccccg gctcattcac caccctggcc tcgcaggtac
    44881 agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg cacatcccgc gcgccttccg
    44941 cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa cagattactg atgctgccaa
    45001 taaccgcatt cactccctga aaggcggttt ccagcatacc gcccggattc agcaggttaa
    45061 gggccgttcc cagggggtca tccagaaagg cggctttttt ttgtgcgaga aactgcgtca
    45121 gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg ggtcggggtg gatcccgggg
    45181 ccgcactggc cacagtgggc tgccatagtt ccgacacaaa atgtgtcacg ccatccacca
    45241 tttcagaacg cccttcttcc gtactgagct ggtcgtgcag agcccgggac atcagcacag
    45301 gccctgtcac cgggttaacc gccagggcta tggcccccgc catttccaga tattccccgg
    45361 ccacatcccg gaacgttctg gcatcctcct gacgctgcgc ttcttcctgt tctgccttcg
    45421 cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc agtcgccgct gcccgcgcgg
    45481 caggtttgct gttgatccat gtgtcaccag agccggtgag gatgtaacct tcaatctgag
    45541 gcgggaaaag catatttgcg agggctgaac aggcttcgct gaccttttct gtcacgccgg
    45601 tgtacaccat cgccacaccc gccacaaaac cggtcagcac gacggccgtg caccccatcg
    45661 tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac aatgagggca tcaataacga
    45721 catttgcggc cacctccagc accgccccca gcacatctgc cataaacgac gtatgcagca
    45781 gggcatcacc ctgtcttgcc gcccagaatg cctcttccat gtcccgttcc ctgtatgatt
    45841 aattcgtcct gacttcctgc cgcatcctga agcgcagatt ctttttaatt tcagcccagc
    45901 gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta ggtgaaggcc atcatgaccc
    45961 gctcacccgg caatacaaat gccacctgac actggtacac ctgctcgtta ccccggagaa
    46021 actggctctg tatttccaaa ccgcctcgtt gagataatat tccgtcataa tgtaaatagt
    46081 ccgttttaat aaaggaatta tttattgaca ggttctgttt ttttaatcat gaatgtaata
    46141 cccatcaccg ctttacagca cactttatct ttccagtcat aatcactaat aatattaagc
    46201 aattagcaat atttacaacg accagagcgg atacatagtt atgtatttaa caatcccaat
    46261 atattcacta tgcaaataaa accactagta acagacacgc tac
//
LOCUS       DAJTYH010000020        45237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTYH010000020 DAJTYH010000000
VERSION     DAJTYH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            61..1749
                     /locus_tag="OO609_001122"
     CDS             61..1749
                     /locus_tag="OO609_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6632620.1"
                     /translation="MQSPLPYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNL
                     SDSKFISSFKSWFESGKIKNIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGE
                     FITYYENGAIESKRHFNNDKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGK
                     LMQENFYQQSVLIGNDTSYYQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKD
                     GKQISVQTWYENGQKEEEKHFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEW
                     YENGIPESLYPYKNGKTDGIAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKE
                     TLYVNGIRQGVEKEFNDRTGKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLS
                     SLDNPTQIKDNATTGDSSAQFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATA
                     YKGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAA
                     QGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            1802..3532
                     /locus_tag="OO609_001123"
     CDS             1802..3532
                     /locus_tag="OO609_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632621.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(3592..4083)
                     /gene="yajQ"
                     /locus_tag="OO609_001124"
     CDS             complement(3592..4083)
                     /gene="yajQ"
                     /locus_tag="OO609_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6632622.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4251..5162
                     /gene="panE"
                     /locus_tag="OO609_001125"
     CDS             4251..5162
                     /gene="panE"
                     /locus_tag="OO609_001125"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6632623.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5125..5715
                     /gene="yajL"
                     /locus_tag="OO609_001126"
     CDS             5125..5715
                     /gene="yajL"
                     /locus_tag="OO609_001126"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6632624.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(5769..7217)
                     /gene="thiI"
                     /locus_tag="OO609_001127"
     CDS             complement(5769..7217)
                     /gene="thiI"
                     /locus_tag="OO609_001127"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6632625.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7423..7665
                     /gene="xseB"
                     /locus_tag="OO609_001128"
     CDS             7423..7665
                     /gene="xseB"
                     /locus_tag="OO609_001128"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6632626.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            7665..8564
                     /gene="ispA"
                     /locus_tag="OO609_001129"
     CDS             7665..8564
                     /gene="ispA"
                     /locus_tag="OO609_001129"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6632627.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            8589..10451
                     /gene="dxs"
                     /locus_tag="OO609_001130"
     CDS             8589..10451
                     /gene="dxs"
                     /locus_tag="OO609_001130"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6632628.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10506..11423
                     /gene="yajO"
                     /locus_tag="OO609_001131"
     CDS             10506..11423
                     /gene="yajO"
                     /locus_tag="OO609_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6632629.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(11534..12052)
                     /gene="pgpA"
                     /locus_tag="OO609_001132"
     CDS             complement(11534..12052)
                     /gene="pgpA"
                     /locus_tag="OO609_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6632630.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12030..13007)
                     /gene="thiL"
                     /locus_tag="OO609_001133"
     CDS             complement(12030..13007)
                     /gene="thiL"
                     /locus_tag="OO609_001133"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6632631.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13085..13504)
                     /gene="nusB"
                     /locus_tag="OO609_001134"
     CDS             complement(13085..13504)
                     /gene="nusB"
                     /locus_tag="OO609_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6632632.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13524..13994)
                     /gene="ribE"
                     /locus_tag="OO609_001135"
     CDS             complement(13524..13994)
                     /gene="ribE"
                     /locus_tag="OO609_001135"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6632633.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14083..15186)
                     /gene="ribD"
                     /locus_tag="OO609_001136"
     CDS             complement(14083..15186)
                     /gene="ribD"
                     /locus_tag="OO609_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6632634.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15190..15639)
                     /gene="nrdR"
                     /locus_tag="OO609_001137"
     CDS             complement(15190..15639)
                     /gene="nrdR"
                     /locus_tag="OO609_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6632635.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            15790..16329
                     /locus_tag="OO609_001138"
     CDS             15790..16329
                     /locus_tag="OO609_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6632636.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            16627..17511
                     /gene="tsx"
                     /locus_tag="OO609_001139"
     CDS             16627..17511
                     /gene="tsx"
                     /locus_tag="OO609_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6632637.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(17688..18035)
                     /locus_tag="OO609_001140"
     CDS             complement(17688..18035)
                     /locus_tag="OO609_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6632638.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18164..19135)
                     /gene="secF"
                     /locus_tag="OO609_001141"
     CDS             complement(18164..19135)
                     /gene="secF"
                     /locus_tag="OO609_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6632639.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19146..20993)
                     /gene="secD"
                     /locus_tag="OO609_001142"
     CDS             complement(19146..20993)
                     /gene="secD"
                     /locus_tag="OO609_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6632640.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21021..21353)
                     /gene="yajC"
                     /locus_tag="OO609_001143"
     CDS             complement(21021..21353)
                     /gene="yajC"
                     /locus_tag="OO609_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6632641.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21376..22503)
                     /gene="tgt"
                     /locus_tag="OO609_001144"
     CDS             complement(21376..22503)
                     /gene="tgt"
                     /locus_tag="OO609_001144"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6632642.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22558..23628)
                     /gene="queA"
                     /locus_tag="OO609_001145"
     CDS             complement(22558..23628)
                     /gene="queA"
                     /locus_tag="OO609_001145"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6632643.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            23721..24294
                     /gene="acpH"
                     /locus_tag="OO609_001146"
                     /pseudo
     CDS             23721..24294
                     /gene="acpH"
                     /locus_tag="OO609_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24299..26116)
                     /gene="malZ"
                     /locus_tag="OO609_001147"
     CDS             complement(24299..26116)
                     /gene="malZ"
                     /locus_tag="OO609_001147"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6632644.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26272..27645)
                     /gene="proY"
                     /locus_tag="OO609_001148"
     CDS             complement(26272..27645)
                     /gene="proY"
                     /locus_tag="OO609_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6632645.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(27721..29040)
                     /gene="brnQ"
                     /locus_tag="OO609_001149"
     CDS             complement(27721..29040)
                     /gene="brnQ"
                     /locus_tag="OO609_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6632646.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29447..30742)
                     /gene="phoR"
                     /locus_tag="OO609_001150"
     CDS             complement(29447..30742)
                     /gene="phoR"
                     /locus_tag="OO609_001150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6632647.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(30800..31489)
                     /gene="phoB"
                     /locus_tag="OO609_001151"
     CDS             complement(30800..31489)
                     /gene="phoB"
                     /locus_tag="OO609_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6632648.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            31679..32881
                     /gene="sbcD"
                     /locus_tag="OO609_001152"
     CDS             31679..32881
                     /gene="sbcD"
                     /locus_tag="OO609_001152"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6632649.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            32878..36021
                     /gene="sbcC"
                     /locus_tag="OO609_001153"
     CDS             32878..36021
                     /gene="sbcC"
                     /locus_tag="OO609_001153"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6632650.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36147..37328
                     /gene="araJ"
                     /locus_tag="OO609_001154"
     CDS             36147..37328
                     /gene="araJ"
                     /locus_tag="OO609_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6632651.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37471..38379)
                     /gene="mak"
                     /locus_tag="OO609_001155"
     CDS             complement(37471..38379)
                     /gene="mak"
                     /locus_tag="OO609_001155"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6632652.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38504..39415
                     /gene="rdgC"
                     /locus_tag="OO609_001156"
     CDS             38504..39415
                     /gene="rdgC"
                     /locus_tag="OO609_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6632653.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39493..39948)
                     /locus_tag="OO609_001157"
     CDS             complement(39493..39948)
                     /locus_tag="OO609_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632654.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(39945..40280)
                     /locus_tag="OO609_001158"
                     /pseudo
     CDS             complement(39945..40280)
                     /locus_tag="OO609_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40435..40719)
                     /gene="ppnP"
                     /locus_tag="OO609_001159"
     CDS             complement(40435..40719)
                     /gene="ppnP"
                     /locus_tag="OO609_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6632655.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(40791..41468)
                     /locus_tag="OO609_001160"
     CDS             complement(40791..41468)
                     /locus_tag="OO609_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6632656.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(41726..41917)
                     /gene="yaiA"
                     /locus_tag="OO609_001161"
     CDS             complement(41726..41917)
                     /gene="yaiA"
                     /locus_tag="OO609_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6632657.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(41967..42491)
                     /gene="aroL"
                     /locus_tag="OO609_001162"
     CDS             complement(41967..42491)
                     /gene="aroL"
                     /locus_tag="OO609_001162"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6632658.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(42674..43132)
                     /locus_tag="OO609_001163"
     CDS             complement(42674..43132)
                     /locus_tag="OO609_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6632659.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43252..44061
                     /gene="proC"
                     /locus_tag="OO609_001164"
     CDS             43252..44061
                     /gene="proC"
                     /locus_tag="OO609_001164"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6632660.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44078..45193)
                     /gene="adrA"
                     /locus_tag="OO609_001165"
                     /gene_synonym="dgcC"
     CDS             complement(44078..45193)
                     /gene="adrA"
                     /locus_tag="OO609_001165"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6632661.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11710 a        11834 c        11120 g        10573 t
ORIGIN      
        1 tctactgtat ggctcgatga aactttaaaa ccaactgcta aacgtctggc aatttatttt
       61 attcaatctc ctttacccta tgacgaggag aaaaaacaat ggaaagtcac cggatggtat
      121 atctcatcaa ctgataatca gtcttcaact aaacagattg catttgaagg atatacagac
      181 acagataacc tctccgattc taaatttatt tcttcattta aatcctggtt tgaaagtgga
      241 aagataaaaa acattgctaa ctataatcca aaaggagaaa gagatggaaa atacgaaagt
      301 tattacaata gcaatggtaa cgtctatgaa tctctaagtt tttctaatgg tcttatcaac
      361 ggtgagttta ttacatatta tgaaaatggg gccattgaaa gtaaacgtca ttttaataac
      421 gacaaaatta aagatggtga atgccctcat ttttatgaag atgggaaaat caaacagcaa
      481 catagctatc tgaatagtaa actcgatggt cctgcatatg aatattatcc tgatggcaaa
      541 ctcatgcagg aaaatttcta tcagcaaagt gtgttgattg gaaacgatac cagttactac
      601 caatccggaa aaataagctc tatacacaat agaaattccc gtggtcagta tgatggagct
      661 aacgaacgtt atagtgaaga gggtaagtta ctgtctaaat ccgtctataa agacggaaaa
      721 caaatttctg ttcaaacctg gtatgaaaat gggcagaaag aagaggagaa acattttgat
      781 gagcaaggcc aattaaatgg tctggttaag caatggtata aaaatggtaa tcttgcaaaa
      841 tcacaaaact ataaacatga catactcgat ggtaacagtg aagagtggta cgaaaatggc
      901 atccctgaaa gtttgtaccc ttataagaat ggaaaaaccg acggcattgc caaatcatgg
      961 aataagtatg gtaaattaac ctattcaatc gaatacaaaa atggcgtaga gaatggggca
     1021 tatcgtaact ggagcaaaaa cacaggtaaa ctaaccaaag aaaccctgta cgtcaatggt
     1081 atcagacagg gagtggaaaa agagtttaac gatcgcacag gtaaactatt aacctccacg
     1141 caatatgtta ataataaaag acatggtacg gaggaaactt atgatcaaaa cggaataaaa
     1201 tatattacat gctatcaaaa tgatcagaaa ttatcgtctt tagacaatcc aacacaaata
     1261 aaagataatg caacaacagg cgattccagt gctcaatttg cattagggaa atacgaattc
     1321 atctgtgcaa atatagatga agggattaaa tggttaacca aatctgcaga gcaaaaaaac
     1381 actgatgcga tatacttttt agctacagcc tacaaaggca atggtatacc tgcaaacaat
     1441 gaaaaatacc tagcatatct gcaacaagct gcaacactgg gcagtagtaa tgcccaggcg
     1501 gaaattggct atttatatct catcggaaaa gaactaccac agaatcttcc tgatgctggt
     1561 gtctggttca aaaaagcagc tgcacaaggt aattttgccg cacatttcta tctgggaagg
     1621 atgtatcaga atggtgacgg cgttgagaga aatatggaaa aagccaggtt tcatctatct
     1681 aatgcggcag aaggtggaat agaacctgcg cttaaagcat taaatgaact tgaacatcaa
     1741 acaaaataaa gcgctcaggg accacgtaag gttcatcaaa cagaaaagga tatagtttgc
     1801 gatgaaagcg aaatccactt tgttattaat gattttatct gttttttatt ctcatcaggt
     1861 tttatccagc gaacccactc agccggatgt ttattctgtc gtcgagaaaa aactaaactc
     1921 tgctttacct ctggaagaaa ataccacctt cagatcgcag gctgaatggt ttaatttgca
     1981 atatgaatta ttagtatctg gctaccctga acgtgcttac aatttactga gcaaacttga
     2041 gacagaatcg aaaaacgcta aatattatct ggatttaact gcctccagag tcgctgaaga
     2101 agaagcacca gagacagcct taactttttt atcaaaaatt gggttaaata aaccggctac
     2161 tcttacatcc tttgagagct atatcaatag ctggataaaa agcaaacaac ccgaaaaggc
     2221 tatgcagctt ctcagcctcg atgagaatgc cctgaattat tatttaccga ctgtgttagt
     2281 cgcatatcag atcgttcctg ataaggcaat aagtatttat aattctgtat atggagataa
     2341 tgttgttata ccctatcagc aagtcaccat gttacttaca gttgcggagg gttatcaaac
     2401 aaaaggcgat ataaaaaacg ccattcatta tgccgataag gcgctgaata tgtttgataa
     2461 agctattgct gtagatagca gtcgtgaatc ccattatagt gatgagtatt tgaaactcat
     2521 gaatatctac gccgccaatg gcaacaaaga aaaagcggtg gttttatctc aacgtctaca
     2581 caaagccatt accgaatctc aatcgaatta cttaagtact ctggaaggaa ttttattgtt
     2641 ttatagaaat aatgatatgc agcaagacta tcaaaacagc ctttcaaatt acatagtatt
     2701 tatcgataaa atttttgcct tttccccatc aacaagaagt gaattaagcc tcattaattt
     2761 attaaattca ttaaatgagg tggaattgat gagaaaacgt ctggcgtttc tgatgacagc
     2821 accagaatac gcttgttata atagtcagta ttgttatgaa gacaaagtca gcgcattaaa
     2881 aatcctgtat cgtcatcatg acaatgagct aatggacaaa tatttcacga ctcttcttaa
     2941 agagatcagt aatcaaccat tagaagactg ggatataata ataagtaata ctggtgaaaa
     3001 atttagtgaa gtgggtctaa ccaaatatgc tcaacatctc gcatctttcg ctgaagaatt
     3061 ttatataaaa gagcaaaaat cacattctga aagtgaaatg agaggactat tttcatcact
     3121 tgccgaatta tatagttttg gtaatgatac tgccagcgca aaccgagtgt tccatcagca
     3181 tgtccctgcc ttcaatacag atcatatgat tcaatatttt ataaatgcca aagaatggaa
     3241 caatgccagg gaattattga taaaagagga aatgttgggt atgcataatc tgatattgtt
     3301 ggagaatatt tgtatgcaaa aaaatgcaga atgcatggca cacattacct ttacgcttaa
     3361 taaattaaca acacaaccag caataacaaa aatagatagc gtgggtaatg agcaacttta
     3421 tcagattggc aaaatctacc ataagttgga aattaaacct gagccagagc aacaggtttt
     3481 aattcagtcg ctttatgacc gggcatcagg aagcgcctct gcaactcaat aaatgcatgg
     3541 tcaaaagggc caccacggtg gccctgacaa tcagtagcta tatccagaaa gttaatcgcg
     3601 gaagtttttg aactggaacg gctgaccgag atcgccaccg cgcaccatcg ccatgacagc
     3661 ctgcaaatca tcacgagatt tgccagtcac gcggatctca tcgccctgaa tttgcgcctg
     3721 cactttcagt ttgctgtctt tgatcatctt gacgattttt ttctgagtgg cgctctcaat
     3781 gccctgtttc agtttcgctt ccacaaacca ggttttaccg ctatgaacga tattttccgg
     3841 tacatccagt gaactgcctt caatgccgcg cttcagcagc ttggcacgca gaatatccag
     3901 caactgattg acctggaagt cggactcgct caacactttg atggttttgc tggcgtcgtt
     3961 cagctcaaat gaggcttcaa cgttacggaa gtcaaaacgg gactccactt cgcgactcgc
     4021 gttatcgacc gcgttacgtg cttcctgaag atcaacttca gagacaatat cgaaagatgg
     4081 catcttttcc tctcccttca tttttgatgc gaagcataat acctgcaaag ttaaataacc
     4141 aacagtcctg caccaggacg tatcccttaa ttgtgcatag tcgcaacagc atgcacaatt
     4201 aagggatagc cttattgaac acccggagtg gttgcgggtg aggaggaaca atgaaaatta
     4261 ccgtattggg atgcggtgcc ttagggcaat tatggcttac agcactttgt aaacagggtc
     4321 atgaagttca gggctggctg cgtgtaccgc aaccttattg tagtgtgaat ctggttgaga
     4381 cagatggttc gatatttaac gaatcgctga ccgccaacga tcccgatttt ctcgccacca
     4441 gcgatctgct cctggtgacg ctgaaagcat ggcaggtttc cgatgccgtc aaaagcctcg
     4501 catccacact gcctgtaact acgccaatac tgttaattca caacggcatg ggcaccatcg
     4561 aagaattgca aaacattcag cagccattac tgatgggcac caccacccat gccgcccgcc
     4621 gcgacggcaa tgtcattatt catgtggcaa acggaatcac gcatatcggt ccggcacggc
     4681 aacaggatgg cgattacagt tatctggcgg atattttgca aaccgtgttg cctgacgttg
     4741 cctggcataa caatattcgc gccgagctgt ggcgcaagct ggccgtcaac tgcgtgatta
     4801 atccactgac tgccatctgg aattgcccga acggtgaatt acgtcatcat ccgcaagaaa
     4861 ttatgcaaat atgcgaagaa gtcgcggcag tgatcgaacg cgaagggcat catacttcag
     4921 cggaagattt gcgtgattac gtgatgcagg tgattgatgc cacagcagaa aatatctcgt
     4981 cgatgttgca ggatatccgc gcgctgcgcc acaccgaaat cgactatatc aatggttttc
     5041 tcttacgccg cgcccgcgcg catgggattg ccgtaccgga aaacacccgc ctgtttgaaa
     5101 tggtaaaaag aaaggagagt gaatatgagc gcatcggcac tggtttgcct cgcccctggt
     5161 agtgaagaga ctgaagccgt caccacgatc aacctcttgg ttcgcggcgg tatcaaagtc
     5221 accactgcca gcgtcgacag cgatggcaac ctggcaatta cctgctcgcg cggcgtgaag
     5281 ctgctggcgg atgcgccgct ggtcgaagtg gctgatggcg aatatgacgt gatcgtgctg
     5341 cctggtggca ttaaaggcac ggagtgtttt cgcgatagca ctctgctggt tgaaaccgtt
     5401 aaacagttcc accgttccgg gcgtatcgtc gcggctattt gcgccgcgcc agccaccgtg
     5461 ctggtgccgc acgatatctt tccgattggc aatatgaccg gcttcccgac gctgaaagac
     5521 aaaattcccg ctgaacaatg gcaggacaag cgcgtcgtct gggatgcacg ggtaaaattg
     5581 ctgaccagcc aggggccggg tacagctatc gactttggtc tgaaaattat cgacctgctg
     5641 gttgggcgcg agaaagccca tgaagtggca tcacaactgg tgatggcggc agggatttat
     5701 aattattacg agtagtgtcg gatgcggcaa acgtcgcatc tgaccagatg cgacgtaaaa
     5761 actacaaatt acgggcgata taccttcaca ttgttaaagc cttgctcgcg cagatagagc
     5821 gcctgcagac ggctcatcac cccgcgctca caccacagca gccaggtttt gttctggtcg
     5881 agatcgccaa atttggtgct cagtttatag aacggcagag aaaccacatc aatcccttcg
     5941 actttcagtg gcttatcttc ctgttcatcg acagaacgga tatcgagaat cacgtcgttc
     6001 gggccgaagc cgttgacggt ttccacttcc accacttcct gctcggtctg ctgggcgatt
     6061 tcgcggatat caacgttatt cgcttcctca accactttat cgagaatact gaagtcgaac
     6121 ttctcttctt ccgcttcaat cttcgattta accgctttca ccgtcgggct tttggagatc
     6181 acgccacaat attccggcat cgtgcgagca aagtcttcgg tgccaatctg gcgagccagg
     6241 ttgatgatgt gctctttgtc gtaagaaatc agcggacgca ggatcagcgt gtcagagacg
     6301 ttatcaatca ggcgcaggtt ggtcagcgtc tgactggaca cctggccgag cgcttcgccg
     6361 gtgaccagcg cctgtacgcc gtaacgttca gccactttag atgcggcacg caccatcata
     6421 cgtttgagga taacgcccat ctgaccgtcg tcgattttct cgagaatttc cccgacaacc
     6481 ggttcgaaat taatggcgac aaaacgcacg cggtgggagc tgccaaagcg gttccacaga
     6541 taatgcgcca cctgacgcac gccaatttca tgcgctgcgc cgccgaggtt aaagaagcag
     6601 taatgcacgc ggcagccgcg acgcatcaac atataactgg aaacaccgga gtcgaaacca
     6661 ccggaaatga gcgacagcac atcttcctgg gtgccaatcg ggaaaccacc aataccttcg
     6721 tagcgacctt taatcagcag gaggcgatcg tcttccactt ccagatgaac agttacatcc
     6781 gggttagtca gcttcacgcg cgcggattca atatgctgat ttaaaccgcc gccgacgtaa
     6841 cgttccacat caatcgagct aaaatcatgt ttgccacggc gcttcacgcg tacgcagaag
     6901 gttttgcctt ccagctgatc gcgatactga accaacgctt tctcaaaaat atcgtgcatg
     6961 tcggtaaacg gcacgtcttc gacttcgaga atatggtgga tacccggaat acgggtcagg
     7021 gcgtcgcgaa tagtcagacg ctggttttca tctttcgcgc gaacttcgat gttatcccag
     7081 tggcggacga cagcgagcgt ctcatcatag tgctttaaaa cgttacgaat gttcccggta
     7141 aggattttta taaagcgcaa gcgcacagat tggcttttga tggtgatttc cgggaacaat
     7201 ttaatgataa acttcatggc ggcaatggtt cgttggcaag ccttaagcga cttgtatagg
     7261 gaaaaataca gcagcccaca cctgcggctg catccaggcg cggaagtata ccactaacat
     7321 cgctttgctg cgcacatcac cttaccattg cgcgttattt gctatttgcc ctgagtccgt
     7381 taccatgacg gggcgaaaaa tattgagagt cagacattca ttatgccgaa gaaaaatgag
     7441 gcgcccgcca gctttgaaaa ggcgctgagc gagctggaac agattgtaac ccgtctggaa
     7501 agtggcgacc tgccgctgga agaggcgctg aacgagttcg aacgcggcgt gcagctggca
     7561 cgtcaggggc aggccaaatt acaacaagcc gaacagcgcg tacaaattct gctgtctgac
     7621 aatgaagacg cctctctaac cccttttaca ccggacaatg agtaatggac tttccgcagc
     7681 aactcgaagc ctgcgttaag caggccaacc aggcgctgag ccgttttatc gccccactgc
     7741 cctttcagaa cactcccgtg gtcgaaacca tgcagtatgg cgcattatta ggtggtaagc
     7801 gcctgcgacc tttcctggtt tatgccaccg gtcatatgtt tggcgttagc acaaacacgc
     7861 tggacgcacc cgctgctgcc gtagagtgta tccacgctta ctcattaatt catgatgatt
     7921 taccggcgat ggatgatgac gatctgcgtc gcggtttgcc gacctgccat gtgaagtttg
     7981 gcgaagcaaa cgcgattctc gctggcgacg ctttacaaac gctggcgttc tcgattctaa
     8041 gcgatgccaa tatgccggaa gtgtcggatc gcgacagaat ttcgatgatt tctgaactgg
     8101 cgagcgccag cggtattgcc ggaatgtgcg gtggtcaggc attagattta gacgcggaag
     8161 gcaaacacgt acctctggac gcgcttgagc gtattcatcg tcataaaacc ggcgcattga
     8221 ttcgcgccgc cgttcgcctt ggtgcattaa gcgccggaga taaagggcgt cgtgctctgc
     8281 cagtactcga caagtacgca gagagcatcg gccttgcctt ccaggttcag gatgacatcc
     8341 tggatgtggt aggagatact gcaacgttgg gaaaacgcca gggtgccgac cagcaacttg
     8401 gtaaaagtac ctaccctgca cttctgggtc ttgagcaagc ccggaagaaa gcccgggatc
     8461 tgatcgacga tgcccgtcag gcgctgaaac aactggctga acagtcactc gatacctcgg
     8521 cactggaagc gctagcggac tacatcatcc agcgtaataa ataaacaata agtattaata
     8581 ggcccctgat gagttttgat attgccaaat acccgaccct ggcactggtc gactccaccc
     8641 aggagttacg actgttgccg aaagagagtt taccgaaact ctgcgacgaa ctgcgccgct
     8701 atttactcga cagcgtgagc cgttccagcg ggcacttcgc ctccgggctg ggcacggtcg
     8761 aactgaccgt ggcgctgcac tatgtctaca acaccccatt tgaccaattg atttgggatg
     8821 tgggacatca ggcttatccg cataaaattt tgaccggacg ccgcgacaaa atcggcacca
     8881 tccgtcagaa aggcggtctg cacccgttcc cgtggcgcgg cgaaagcgaa tatgacgtat
     8941 taagcgtcgg gcattcatca acctccatca gtgccggaat tggtattgcg gttgctgccg
     9001 agaaagaagg caaaaatcgc cgcaccgtct gtgtgattgg cgacggcgcg attaccgctg
     9061 gcatggcgtt tgaagcgatg aatcacgcgg gcgatattcg tcctgatatg ctggtggtcc
     9121 tcaacgacaa tgaaatgtcg atttccgaaa atgttggcgc gcttaacaac catctggctc
     9181 agctgctttc cggtaagctt tactcttcac tgcgcgaagg cgggaaaaaa gttttctctg
     9241 gcgtgccgcc cattaaagag ctgctcaaac gtaccgaaga acatattaaa ggcatggtgg
     9301 tgcctggcac gttgtttgaa gagctgggat ttaactacat cggcccggtg gacggtcacg
     9361 atgtgctggg gcttatcacc acgctaaaga acatgcgcga cctgaaaggc ccgcagttcc
     9421 tgcatatcat gaccaaaaaa ggtcgtggtt atgaaccggc agaaaaagac ccgatcactt
     9481 tccacgccgt gcctaaattt gatccctcca gcggttgttt gccgaaaagt agcggcggtt
     9541 tgccgagcta ttcaaaaatc tttggcgact ggttgtgcga aacggcagcg aaagacaaca
     9601 agctgatggc gattactccg gcgatgcgtg aaggttccgg catggtcgag ttttcacgta
     9661 aattcccgga tcgttacttc gacgtggcta ttgccgagca acacgcagtg acctttgccg
     9721 ccggtctggc gattggtgga tacaaaccca ttgtcgcgat ctactccacc ttcctgcaac
     9781 gcgcctatga tcaggtgctg catgacgtgg caattcaaaa gctaccggtc ctgttcgcca
     9841 ttgaccgcgc gggcattgtt ggtgctgacg gtcaaactca ccagggcgct tttgatctct
     9901 cttacctgcg ctgcatacca gaaatggtca ttatgacccc gagcgatgaa aacgaatgtc
     9961 gccagatgct ctataccggc tatcactata acgatggccc gtccgcggtg cgctacccgc
    10021 gcggcaacgc ggttggcgtg gaactgacgc cgctggaaaa actaccaatt ggcaaaggca
    10081 ttgtgaagcg tcgtggcgag aaactggtga tccttaactt tggtacgctg atgccagaaa
    10141 cggccaaagt cgccgaatcg ctgaacgcta cgctggtcga tatgcgtttt gtgaaaccac
    10201 ttgatgaagc gttaattctg gaaatggccg ccagccatga agcgctggtc accgtagaag
    10261 aaaacgccat tatgggcggc gcaggtagcg gagtgaacga agtgctgatg gcccatcgta
    10321 aaccagtacc cgtgctgaac attggcctgc cggacttctt tattccgcaa ggaactcagg
    10381 aagaaatgcg cgccgaactc ggcctcgatg ccgccggtat ggaagccaaa atcaaggcct
    10441 ggctggcata atccctactc cactcctgct atgcttaaga aattattcat ggcaggagtg
    10501 gcagcatgca atacaacccc ttaggaaaaa ccgaccttcg cgtttcccga ctttgcctcg
    10561 gctgtatgac ctttggcgag ccagatcgcg gtaatcacgc atggacactg ccggaagaga
    10621 gcagccgtcc cataattaaa cgcgcgctgg aaggtggcat aaatttcttt gataccgcca
    10681 atagctattc cgatggtagc agcgaagaga tcgttggtcg cgcactgcgg gatttcgccc
    10741 gtcgtgaaga cgtagtcgtt gccaccaaag tgttccatcg cgttggtgat ttaccggaag
    10801 gattatcccg tgcacaaatt ttgcgctcta tcaacgacag cctgcgacgt ctcggcatgg
    10861 attatgtcga tatcctgcaa attcatcgct gggattacaa cacgccgatc gaagagacgc
    10921 tggaagccct gaacgacgtg gtaaaagccg ggaaagcgcg ttatatcggc gcgtcatcca
    10981 tgcacgcttc gcagtttgct caggcgctgg aactacaaaa acagcactgc tgggcgcagt
    11041 ttgtcagtat gcaggatcac tataatctga tttatcgcga agaagagcgc gagatgctgc
    11101 ctctgtgtta tcaggagggc gtggcggtga ttccgtggag cccgctggcg cgggggcgac
    11161 tgacgcgtcc atggggagaa actaccgcac gactggtgtc tgatgaggtg gggaaaaatc
    11221 tctataaaga aagcgatgaa aatgacgcgc agatcgcaga gcggttaacg ggcgtcagtg
    11281 aagaactcgg tgcaacacga gcacaagttg cgctggcctg gttgttaagt aaaccgggca
    11341 ttgccgcacc gattatcgga acttcgcggg aagaacagct tgatgagctg ctgaacgcgg
    11401 tggatatcac tttgaagcca taacagattg ccgagctgga aacgccgtat aaaccgcatg
    11461 cggtcgtagg atttaaataa gagtccaggc ctgatgagac gtgacaagcg tcacatcagg
    11521 catcggtgca caactacgac agaataccca gcggccagtg atgaccgata aagtacagga
    11581 tgcctgcgga aatcaccccg gcgacaatat cgtcgatcat gatccccatg ccgccatgta
    11641 cattgcgatc aaaccagcgg atcggccacg gcttccacat atccagaata cggaaaatca
    11701 caaacccggc ggcaacccac tgccagtcat tggtcggcag cgccatgagc gtgatccaca
    11761 taccaataaa ttcgtcccag acaatgctgc cgtgatcgtg cacgcccata tctttcgccg
    11821 tttgatgaca aagatagacg ccgatacaga tccccaacat caccaccagc gagtagagct
    11881 gccagggtaa aaaggtcatc agataccaga aagggatcgc cgccagcgat cccatcgagc
    11941 caggaacgat tgggcttaat ccacttccga atccgacagc aagcagatgc cacggattac
    12001 tcatcttcag gcgacttttc gcgacatctt tatggcgtga caaaatggtc atatcctttc
    12061 cagtcaaacg tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc
    12121 atttgcccga tacaggtaaa cggtacgcca aggtgtccga gagcaacatc cagcgcgcca
    12181 cggttcagtt ccggcaccgt gaaacacaac tcgtaatctt caccgccaga gagcgcccag
    12241 cgcagcgcct gttccggttc aacatggcga gaaagcgcat cagaaaacgg cagcaatgcc
    12301 aggtcaatac gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga
    12361 ccgtcagaga gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata
    12421 cgcggcgatg gacggagatg acgtttgatc aagtagtcag catctttagc atcggcaacc
    12481 tgcaaacggt tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt accggtcaca
    12541 tagatccagt cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag
    12601 ccgtggatac ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc
    12661 atatcgtaat aattgagaag atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg
    12721 tctacgtccg gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc
    12781 agatcgctta ggttcaccgc cagtgcttta taagccagat cagcaggatc gatatcaggg
    12841 aggaaatggt tgcccgccac cagcgtatca gtgctgatcg ccagggtctg tttctcgggg
    12901 atattgagaa gagcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt
    12961 acacggtcaa aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca
    13021 gcaggtaaaa aagaaaaggc cggcgatcat ggaacagtct tccgtgaatc taccggcctg
    13081 gatattactt tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga
    13141 acttatggct gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca
    13201 ctttgtatgg cacatcacta cgtttagaca gttcgtacag cgcaatgcgc agtactgctt
    13261 tttctacctg gcccagttct tccagcaggc gggacaggta gggcttcatc agtccgtcga
    13321 ggtatgcggt gttagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt
    13381 cttttacatc ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca
    13441 actgccagga gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt
    13501 tcacggattt ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca
    13561 agcgcggtca gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc
    13621 gcttgttcaa tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg
    13681 gcaacatgcg ccaggccgtt gcttgcacca ccagcgacat attcaaagtg ggcagtgcca
    13741 ccacgaataa ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca
    13801 cccgccgcca gcggcagctc ataggcacca ggcacccaaa caacggtaat gttttcatct
    13861 tttacctgac cgatacgttt cagtgcgtca attgcacctt ccagcaggct gtcattgata
    13921 aagttgttga aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct
    13981 tcaataatgt tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt
    14041 agcataatat ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg
    14101 caggcaaaca tccgggccta catgacgtat ctctttgaat ttaaattggg gggcgtcggc
    14161 taatttctca agccctggca gcgagcataa tccacgggcg tcgctgccta atagtttagg
    14221 tgcgatatag acaatcagct catcgactaa acccgcctgt agcaatacgc cagcgagcgt
    14281 tggccccgct tccacccaga tgctgttaat ctgctgttta cccagttgca tcatcagtac
    14341 aaccagatcc agatgacctt tatgctctgg aatcagcaag gtacgcaccg tttccggcca
    14401 ctcacgagaa tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgcg
    14461 atgttccggc gtcacgcgat tttggctatc aatcacaata cgtactggct ggcggagatt
    14521 ttgttgcggg tagagcgcct gagtttgttc atccagttca gaccaacgca ctgttaaggc
    14581 tggatcatcc gccagcaccg tggcgctgct ggttaaaatg gcatgacttt gtgcgcgctg
    14641 tcgttgtacg tcgcgccgcg cctgaggcga agtgatccac tggctttcgc cgctcgccat
    14701 cgccgtgcgg ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg
    14761 catccgcttg agaaagcctt tattcaattg ctcggcttca ctcatcatca gcccgtggct
    14821 gacgtcaatg ccagcctgtt gcagacggta aagtccacgc ccagcgacct gcgggttagg
    14881 atcttgcatc gctgcgacca cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg
    14941 tggcgtacgg ccatgatggc tacagggttc gagtgtcaca tacgccgttg cgcctttggc
    15001 tttttcaccc gccatacgca acgcgtgtac ttcggcatgt ggttcacccg ctcgttggtg
    15061 gtaaccttca ccgacaattt cgccatcttt gacaatgacg cacccgacat tcgggttggg
    15121 gtgggtggta aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc
    15181 ctgcacggct tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa
    15241 actgcggtag acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc
    15301 catcaccaga ttgccaatca tcttgctcgg tacttcgcgc tcaccggtgg cgcgcagctg
    15361 cgatttaata tgattgattg ccatttcaac gtaatcggaa ctcaccggac gtttttccag
    15421 cgcccgcagc attccgctac gcaatttctc ttcattaaac ggttcacgca cgtcgttgct
    15481 ttttacaaca cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca
    15541 caccagacac tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat
    15601 taccttagtg tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt
    15661 taacggaaat ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat
    15721 aaaggatcga tgctttcgct gcttttaccc tctaccatta ggctaattgc gacgagaaag
    15781 ggacgaccta tgaatactaa cgtttttcga ctgctcctgc tgggaagcct gttcagcctt
    15841 agcgcctgtg tgcagcaaag tgaagtgcga cagatgaaac acagcgtcag cacgctgaac
    15901 caggagatga cgcaactcaa tcaagaaaca gtcaaaatca ctcagcaaaa caggctgaat
    15961 gcaaaatcca gcagtggggt ttaccttctg cctggagcga aaacaccggc aagactggaa
    16021 agccagatcg gtactttacg tatgtcgctg gtgaatatta cgcctgatac agatggcacc
    16081 acccttacac tacgtattca gggtgagtcg aatgatccct tgcccgcatt cagcggaact
    16141 gttgaatatg ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtgcagaat
    16201 caattgatta atgcccctgc cagcgtcctc gcccccagcg atgttgatat tccgttacaa
    16261 ttaaagggga tttctgtgga tcagttaggc tttgtacgta tccacgacat tcaacctgtt
    16321 atgcagtaaa cgtatttcgg gacgattttg tgcgtcccgc aacatcttcc ccgtcatttt
    16381 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    16441 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    16501 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    16561 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    16621 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    16681 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    16741 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    16801 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    16861 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    16921 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    16981 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    17041 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg atatcgacac tggcctgccg
    17101 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatgg cgcagcgaac
    17161 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    17221 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    17281 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    17341 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    17401 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    17461 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    17521 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    17581 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    17641 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    17701 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    17761 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    17821 tcatgatcgt ggcaatagag acacaacaat tcccagttac tgccatcttc cgggttattg
    17881 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttagaata aacaaactcg
    17941 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    18001 tctaaccgtg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    18061 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    18121 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattaaggc agaattgacg
    18181 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    18241 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    18301 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    18361 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    18421 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    18481 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    18541 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    18601 gcgtcagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    18661 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtcagccc
    18721 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    18781 taatcacctt cagaacttgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    18841 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    18901 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    18961 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    19021 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    19081 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    19141 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    19201 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    19261 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    19321 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    19381 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    19441 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    19501 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    19561 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    19621 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    19681 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    19741 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    19801 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    19861 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    19921 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    19981 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccagt cagaattacg
    20041 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    20101 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    20161 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc gggcagttca
    20221 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    20281 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    20341 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    20401 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    20461 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    20521 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    20581 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    20641 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    20701 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    20761 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    20821 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    20881 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    20941 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    21001 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    21061 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    21121 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    21181 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    21241 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    21301 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    21361 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    21421 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    21481 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    21541 gcaacggtcg agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    21601 ctcaggatcg agtgggccag tatcgctctt atatttcgca ttgcggattt tcaccacgcc
    21661 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    21721 atcgataccg cgacgtacgc cttcaaccag gtcttccggt ttaccaacgc ccatcaggta
    21781 acgcggtttg tctgccggaa tttgcggaca cacatgctcc agaatacggt gcatatctgc
    21841 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaagccga tatctaccag
    21901 acctttaaca gatatatcac gtaaatcttc gtaaatactg ccctgaatta taccaaataa
    21961 cgcatttttg ttaccaagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    22021 catctccata gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    22081 gatcatgacg atatccgaac caagatcgta ctgaatctcc atcgactttt ccgggtcgag
    22141 gaaaatcggg tcgccgttga tcgggttacg gaagtgaacg ccctgttcgg tgattttacg
    22201 aatatcacca aggctgaaga cctggaagcc gccggagtcg gtaagaatcg gtcctttcca
    22261 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    22321 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    22381 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    22441 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    22501 cattttttct ccactacgtc agaaaaacag tccaacgttt aaaccagcgc cgcggaatta
    22561 ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg catcaccgta
    22621 actaaaaaag cgatatttct cttctaccgc cgctttatag gcattcatgg tgtgttgata
    22681 accggcaaag gccgaaacca gcataatcag cgtcgactct ggcaagtgga agttcgtcac
    22741 cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg tatcgtcaaa
    22801 gaacggttcg ataagatcgt tttttgccgc ctgtgccgcg ctttccagcg aacgtactga
    22861 agtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta ccgcgtctac
    22921 cacatcctgc ggtacttcag cgtattccga gtgcatgatg tgatcttcaa tggtgtcgac
    22981 gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc acaaacgcca tctccacgcc
    23041 tttggcgcgt aatttttcca gcaaaggctc gtcaaaatgc agaccagcgg tcggggctgc
    23101 aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag cgtcttcgtc
    23161 cggacgatcg atatacggcg gcagcggcat atggccgatg ctgttgagga tatccagtac
    23221 cgagcgttca tcattaaatt cgacttcaaa cagtgcgccg tggcgcgcgg tcattgttgc
    23281 gttgatactt tcgtcatcgc ccagcaacag ttctgcgcca ggttttggcg ctttcgaggc
    23341 gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgc tcaaccagca cttcaatctt
    23401 gccgccgctg gctttacgcc caaacagacg cgccgggatc acgcgggtat tattaaaaac
    23461 cagaagatcg ccggggttga gcttatcgag taaatcggtg aaagtaccgt gcgtcagcgc
    23521 gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca tgggatagtg
    23581 ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga cactgactca
    23641 gactaaaata agagtcgggt agtctagtgc cggggcgctc tccctgcaac ctttaccccc
    23701 ctttggatag aatataatcg atgaattttt tagctcacct gcatttagcc catctcgcgg
    23761 aaagctcgct ttctggcaat ttactggctg atttcgtacg cggaaatcct gaagaaagtt
    23821 ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tattgactga caatctgccg
    23881 gaagtccgcg aagcacagga gtggtttcgt agtgaaacgc gccgcgttgc gcctattacg
    23941 ctggatgtca tgtgggatca ctttctttcc cgccactggt cgcagctgtc gccggacttt
    24001 ccgctacagg aatttgtctg ttatgcccgc gagcaagtga tgacgatttt gccggactca
    24061 ccgccacgtt ttatcaatct gaacaattac ttgtggtcag agcagtggct ggtgcgctat
    24121 cgcgatatgg atttcatcca gaacgtgtta aacggcatgg caagccgccg cccacgtctg
    24181 gatgccctgc gtgactcctg gtacgattta gacgctcatt atgacgccct cgaaacccgc
    24241 ttctggcagt tttatccgcg gatgatggcg caggcgtcac acaaggcgtt ataaaacgtt
    24301 agttcatcca taccgtagcc gaaatggcag gcaaagccag aatcccgtca gtcagttgcc
    24361 catgcccttc tttgcattgc cattgcacgg cattgagaaa cggtgacgcg ggtagcacca
    24421 cttcactggc ctcgccacgg ttgattgcca ccagtacacg ttgctgattc agcacgcgga
    24481 caaataccac tacgttatct tccgcataca gcacctgaca gccgccacga cgcagcgcct
    24541 gacttttctt acgcagcaca atcattcgcc ggtacagcgc gaataacgcc gtatcctgct
    24601 tttccacctg ccaggggaac ggtttacggc aaaacggatc gtttttacca tccagtccca
    24661 cttcatcacc gtaataaatg cacggtacac caggccaggt gaacagccag accaccgcca
    24721 gcggcaggcg cgcaatatcc cgaccgagca gcgttttaaa tcgcgcagta tcgtggctat
    24781 cgagctgatt aaacatacgt aattgttgtt gatgagaaag ccctgcgcgg taattatcca
    24841 tccaggccat acaggtttgg gcgtcaattt gctgcggatc ataagagatg tcggtattgg
    24901 caagaaatcc ccacaacggg aatgtgaagc cacgatagtt catggcagca tcttccacat
    24961 cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc gacaatatac gcttccggct
    25021 gggtttcttt cgccgcttcg gtgatcccgg caacgtgctg catattattg cgcgccccac
    25081 ccgcctcccc cagcatatgc accacatcca gccgccagcc gtccatattc cacggcgctt
    25141 tcagccagtg gcggacaata ctgtcttccc cgcgataaat ttcattcacc agactttccg
    25201 actgataatc cagcttcgtc aagctggcat agccaagcca gtcgagcgcc gtgccatcat
    25261 cactaaacga gtaccagtcg cgccagggcg attcagagtt gtgacaagct ccccccgtgc
    25321 caagattgtg cctgtcaaac caggcatggg aatcgccact gtggttaaac acgccgtcca
    25381 gcaccagccg cattcccagc tgctgcgtat tgtgtcgcaa acgcagcaac gccccatcac
    25441 cgccaaactg cggatcgaca tggcgataat cctcggtatc gtatttatgt acgctgggag
    25501 ctttaaacac cggattgaga tacagcgctg tcacgccaag ctttttcaga tacggcagtt
    25561 tttcgcttat cccgtccaga tcgccgccat agaacgttga tccgcccgcc tgcgccgtga
    25621 ccggttcatc ccagtcacgc aagatgatct cttgtccggc tgcatgatgg taatagacat
    25681 gatcctgttc agcttcacga ggaagactac gcgcaaaacg atcagggaag atctgataaa
    25741 aaatctgatc cgcagcccat tgtgggccga tatcaggtac atcgacggca aactgctcca
    25801 gtcgtgccgg cggcattcgg ctgaagccct gcggtgtaaa ccaacgctgg cgatcgtgcc
    25861 acagcagttt gaaactgtaa cgccgccggg gttgtccgct ggagagatca atcgccgcac
    25921 gccatgcggt gacgcctggc tgcggctgac tgcgctgctt atgcattggt actgacattt
    25981 cttcgttatc gtgttctgta cgcagcataa tgcgctgcgg tgggtcttcg cccgtcagcc
    26041 acagtgtaat gagcagttga tctttgcttt gtttaacaaa tgggggcacc ggcaggtgcc
    26101 atgcatttaa catcataatt cccctgtgat gaaaatctga tcaccttgcc atagcctaat
    26161 gcaggatcac tcatcatctg aaggatattt gggggaggag gacgggggcg gataactttc
    26221 ggaatagccg gatcaggatt tcctcctgaa ccggcttaca gaacgcaagt attattggtt
    26281 ttcagccagc tgacgatcgt ggcggcgttt aaacatccag ccaatcaaca gcacaacaat
    26341 ccacgcgaaa ccgacataca gcgagatacg cgtatccggg tgataaccaa tcaacccgat
    26401 aataaagagc aggaaaatca gcccgccgat ggtcgttgct accccacccg gcactttaaa
    26461 tttcagcgcc ttaacttctt ctggcggcaa acggcgacgg aaggcgattt gcgacagcag
    26521 gatcataatc cacacccata ccgtggcgaa ggttgccagc gaagcgatca ccaggaagac
    26581 gttttccggc atgatgtagt tcagatacac cgcaaacagc agcgcggtag tcatcaccag
    26641 caccgtaacc cacggaatac cgcgacgcga cgttttgctg aaaattttcg gtgcgctgcc
    26701 ctgctctgcc ataccgtgga gcatacggcc tacgccaaat acgtcactgt taattgccga
    26761 cagcgaagcg gtcagcacaa caaagttaag aatgctggcg gcaaaggtaa tgcccatatg
    26821 ctggaacgtc agtacgaacg ggctaccggc agtgccaacc tgattccacg ggtagataga
    26881 cataatgacg aacagcgtac cgacgtagaa caccagaata cgcatcggca cggagttaat
    26941 cgcacgcgga atcgatttct caggatcttt cgcttcaccg gcggtaatcc cgataatttc
    27001 gatcccaccg taagcaaaca tcaccatttg caacgacatt accatgccaa gccagccgtt
    27061 actgaagaag ccgccgttgc tccacaggtt atgaataccg gtcggttgcc cgccgttgcc
    27121 aatcccccag atgatgatgc cgaaaccggc gacaatcatg atgatgatgg tggcgacttt
    27181 aaagaacgag aaccagaatt ccagctcacc gaagaccttc acgctcatca ggtttacggc
    27241 gcaaatgatc agcaccacgc tcagtaccca aatccagtgc ggcaccgtcg ggaacaagac
    27301 acccatatag ataccaaaag cggtcacatc ggcgatggcg acaataagga tttcaaaaca
    27361 gtaggtccat ccggtaatgt aacctgccag cgggccaagg ttttcctgcg cataacgcga
    27421 gaaagagctg gcggccgggt tatgtaccga catttccccc agcgcacgca taatgatata
    27481 cgccgcgata ccaccgataa tataggccaa caacacgctc ggaccggcca ttttgatggc
    27541 gtctgccgaa ccgtaaaaca gcccggtgcc aattgctgaa cccagtgcca taaagcgtat
    27601 gtggcgggta cttagcccac gctttagctt attcttactt tccatcaatt cctaacccat
    27661 caacacaata aaaaaccacg gggcatcgca gccccgtggt taaaacaaat gttcagtgat
    27721 ttagtgagcg ctggaggtca cctgacgacc tgccgcgcga tcccagataa tggccagaac
    27781 caccatcacc actgttggca ttaaccacgc cagaccttgt tcggccagcg gtaaacgctg
    27841 cgcccaggac ggtaagatat cgctgaatgc agatgctttg atcccgtcga gaataccaaa
    27901 aagcaggctg ataaacatcg gcggagcaat cacgcgggac gaattatgcc accatgagcg
    27961 tgtaaaactt aatacaacca gtgcgataca cggcggataa atagcggtca gcaccggtac
    28021 ggagatctgg atcagctggc ttaagccgag gttagacacc accatcgaga agccgccgag
    28081 gataaacacc agcgtacgat aagagagcgg tacgtactgg gcaaagaatt ctgcacaagc
    28141 acaggtcagg ccaaccgccg tgaccagaca ggcgatgaag attaacgccg ccagcaggaa
    28201 gctaccgccg ccgccaaagg tgtgctgaac gtaagcatgc agaatagcag cgccgtttgc
    28261 agactgatcg accagcgacg cgctgtctga acccagacag aacagcgcca ggtacagcaa
    28321 agtcagacca acacccgcca tcaggccagc ccagacggta taacgggtca gcagacgcgc
    28381 ttcggtaacg ccacgagaac gcgccgcgtt aacaataacg ataccaaaca ccattgcgcc
    28441 cagcgtatcc atggtcagat agccgttaac gaagccgtta gaaaatgcag cgttttgata
    28501 agcctcagtc gccgtgctga tagaacccgc cggccagaca atcgcgacaa cagacaggat
    28561 gaccagcgcg ataattttca gcggcgcaag gaagttgccc acggtatcca gcagcttgcc
    28621 cggatagagc gaaaccagaa taacgatagc gaaatagacc aggctgtaga taaacagcgg
    28681 cagcgcggaa tcacccgtca gcggcgcaat ccccacttca aaggaaacgg tagctgtacg
    28741 cggcgtagcg aaaagcggcc ccaccgccag gtaacaaacc gttgccagca gtacgccagc
    28801 gactttaccg attggcgtgc tgaggctgtc aacaccgccg ccaacttttg ccagcgccac
    28861 taccgttaat accggcaggc caacggcagt aatgaggaag ccgaatgccg cagtccagac
    28921 gtgttcgcct gcctgtaagc cgaccattgg agggaaaata atgttacctg cgccgacgaa
    28981 caacgcaaat gtcataaagc ccagagcgat gatatcgcgc gatcttaatt gatgggtcat
    29041 aaatcatact gcctgtggat gtggtgttgt aacgttaaat ttttgtgcca atccctgggg
    29101 atgatacagc ggtatattta aacaatttta gcgggaaata ctcccaacca ggcgcggcga
    29161 agaatagttt ttcgatttac cgcgttaatg cagtcagtat gacagcacct gaagcgcaat
    29221 ttaaacgctt ataacgtttt aaagcaagga gggatctaaa aaccagaggt atatagcgat
    29281 aagaaatcac caaccagtat aaaacactat ttccgtgaaa aagacatggc taatcatgcg
    29341 aacaaaaaat caccaaccgt tgtaaaatct aacgcccgta ttttgactgc aaagaaaata
    29401 agccagcatc ggctggctta tggcaataaa agatgacaaa ggcggattaa tcgctgtttt
    29461 tggcaattaa acgttccggg ataacaaaac tgaaacgtgt tccttttcct actgtactct
    29521 caatattcag gcgactttcg tgatgattca ccgcatgttt cacgatcgct aaccctaatc
    29581 cgctaccgcc ggtttgccgg gaacgcgctt tatcaacgcg ataaaaacgc tcggtcaggc
    29641 gcggaatatg ctccggtgca atgcccggtc cgttatcttc aacgctaaat tcggcaccgt
    29701 gcggcactcg ctgccagcgt acggtgatat gcgtgccttc cggcgtatga ttcacggcgt
    29761 tatagaccag gttcgaaatc gcactgcgta gctgatcttc gttgccagac accttgaggc
    29821 cgttatctat ctcaaaggta aatgtctgtt ttttctgact cagagtctga gcctcgcgct
    29881 caacaacgcg cagcatcatc ggcacatcaa ccttttcatt gagcaattgc gtcggcgcgg
    29941 cttcaatttt cgacagcgtt agcaactgct tcaccagccc ttccatccgc tgggtctgct
    30001 cgcgcatggt gtgcaacgct ttttcgcgta ccgcgccttc cagcggctgc tcatccatca
    30061 tctccaggta accctgtaac acggtcaatg gcgtacgtaa ctcatggctc acgttggcaa
    30121 aaaagttacg ccgcgcccct tccagttgat gcatttgcgt gacatcacgc gccaccatca
    30181 gcaactgttt gtgggtataa ggcatgacgc gaatttccag atgccgcccg gtgttgagca
    30241 ccagattgag cgggcgagaa aaatcacgcg ttttcagata ttgcgtaaac tccgggtaac
    30301 gcagtaggtt aaggatgttc tgcccgttat cttccggcca gcgcaaacca agaatttgtt
    30361 gcgccagacc gttacaccag aaaataccgc cctcttccgt ggtcagcacc accgcgtcgg
    30421 gcagcgactc cgcgccgcta cgaaagcgtt taatcagatt gcccagttca cggcggcgtt
    30481 ttttatttcg cagctgcatc tggtgtaagc cgtatagtag cggttcccag ctaccacgcc
    30541 ccggtggcgg ggtcatactg cgatccaccc acagccacca tgaaaggcgc aataaattcc
    30601 agaaatgcca gataagcagt cctgttaccg atgccagcaa aaaccagggc aggtaaccaa
    30661 aaaatgcacc caggatgaaa gccgggaggc agcaaagtag cagctccagc accagccttt
    30721 tccacgacag ccgttccagc acgtaagata ctccagttaa gaaatcataa gccctgctct
    30781 gcgtccgatg agcaaggcgt taaaagcggg ttgaaaaacg atatcctgta ccgcgcacgg
    30841 tctgcaccat gcggtcatgc ccgccgggct ccagtgcttt acgcaggcga cgaatgtgga
    30901 catcgaccgt gcggtcttcc acatacacgt tagttcccca gacgtggttt aacagctgct
    30961 cgcggctgta cacgcgctca ggatgcgtca taaaaaagtg cagcagttta aattctgtcg
    31021 gccccatctc cagcggctct tcgcccgcca tcactcggtg agatgtcggg ttgagactta
    31081 atccctgcat cttaatcacc tcttccaccg ccattggcga aatacggcgc attaccgctt
    31141 tgattcgcgc caccagctcc ttcggcgaaa atggcttggt gatatagtca tccgcgccgg
    31201 tttcaaggcc gcgcacgcga tcttcttctt cccctctggc ggtcaacatc accactggaa
    31261 tatcccgggt catcgactcg cgcttgaggt gtttgatgaa ctggataccg gagccgccag
    31321 gtaacatcca gtcaaggaga attaaatccg gccagggttc attcagttga ttcacagcac
    31381 tgtcataatc ttccgcttcg accggctgaa agccattttg ttcgagcacg aagcagacca
    31441 tttcgcgaat tggagcttca tcttctacga ccagaatacg tctcgccatg atttgccctg
    31501 ttgtaataaa taggttgcga tcattaatgc gacgtcatta tgcgtcagat ttatgacaga
    31561 tttatgaaaa gctcgtcgca catatcttca ggttattgat ttccgtggcg cagaaaaaag
    31621 caaatggcac atctgtttgg gtataatcgc gaccatgctt tttcgccagg gaaccgttat
    31681 gcgcatcctt cacacctcag actggcatct cggccagaac ttctacagta aaagccgcga
    31741 agctgaacat caggcttttc ttgactggct gctggagaca gcacaaaccc atcaggtgga
    31801 tgcgattatt gttgctggtg atgttttcga taccggctcg ccgcccagtt acgcccgcac
    31861 gttatacaac cgttttgttg tcaatttaca gcaaactggc tgtcatctgg tggtactggc
    31921 aggaaaccat gactcggtcg ccacgctgaa tgaatcgcgc gatatcatgg cgttcctcaa
    31981 tacaaccgtg gtcgccagcg ccggacatgc gccgcaaatc ttgcctcgtc gcgacgggac
    32041 gccaggcgca gtgctgtgcc ccattccgtt tttacgcccg cgtgacatta tttccagcca
    32101 ggcggggctt aacggtattg aaaaacagca gcatttactg gcagcgatta ccgattatta
    32161 ccaacaacac tatgccgatg cctgcaaact gcgcggcgat cagcctctgc ccatcatcgc
    32221 cacgggacat ttaacgaccg tgggttccag taaaagtgac gccgtgcgtg acatttatat
    32281 tggcacgctg gacgcgtttc cggcacaaaa ctttccacca gccgactaca tcgcgctcgg
    32341 gcatattcac cgcgcacaga ttattggcgg catggaacat gttcgctatt gcggttcccc
    32401 cattccactg agttttgatg aatgcggtaa gagtaaatat gtccatctgg tgacattttc
    32461 aaacggcaaa ttagagagcg tggaaaacct gaacgtaccg gtaactcaac ccatggcggt
    32521 gctgaaaggc gatctggcgt cgattaccgc acagctggaa cagtggcgcg atgtatcgca
    32581 ggagccacct gtctggctgg atatcgaaat cactactgat gagtatctgc atgatattca
    32641 gcgcaaaatc caggcattaa ccgaatcatt gcctgtcgaa gtattgctgg tacgtcggag
    32701 tcgtgaacag cgcgagcgtg tgttagccag ccaacagcgt gaaaccctca gcgaactcag
    32761 cgtcgaagag gtgttcaatc gccgtctggc actggaagac ctggatgaat cgcagcagca
    32821 acgtctgcag catcttttca ccacgacgtt gcgtaccctc gccggagaac acgaagcatg
    32881 aaaattctca gcctgcgcct gaaaaacctg aactcattaa aaggcgaatg gaagattgat
    32941 ttcacccgcg agccgttcgc cagcaacggg ctgtttgcca ttaccggccc aaccggtgcg
    33001 gggaaaacca ccctgctgga cgccatttgt ctggcgctgt atcacgaaac tccgcgtctc
    33061 tctaacgttt cacaatcgca aaatgatctc atgacccgcg ataccgctga atgtctggcg
    33121 gaggtggagt ttgaagtgaa aggtgaagcg tatcgcgcgt tctggagcca gaatcgggcg
    33181 cgtaaccagc cggacggtaa tttgcaggtg cctcgcgtag agctggcgcg ctgcgccgac
    33241 ggcaaaattc tcgccgacaa agtgaaagat aagctggaac tgacagcgac gttaaccggg
    33301 ctggattacg ggcgcttcac ccgttcgatg ctgctttcgc aggggcaatt tgctgccttc
    33361 ctgaatgcca aacccaaaga acgcgcggaa ttgctcgagg agttaaccgg cactgaaatc
    33421 tacgggcaaa tctcggcgat ggtttttgag cagcacaaat cggcccgcac tgagctggag
    33481 aagctgcaag cgcaggccag cggcgtcacg ttgctcacgc cggaacaagt gcaatcgctg
    33541 acagcgagtt tgcaggtact tactgacgaa gaaaaacagt taattaccgc gcagcagcaa
    33601 gaacaacaat cgctaaactg gttaacgcgt caggacgaat tgcagcaaga agccagccgc
    33661 cgtcaacagg ccttgcaaca ggcgttagcc gaagaagaaa aagcgcaacc tcaactggcg
    33721 gcgcttagtc tggcacaacc ggcacgaaat cttcgtccac actgggaacg catcgcagaa
    33781 cacagcgcgg cgctggcgca tattcgccag cagattgaag aagtaaatac tcgcttacag
    33841 agcacaatgg cgcttcgcgc gagcattcgc caccacgcgg cgaagcagtc agcagaatta
    33901 cagcagcagc aacaaagcct gaatacctgg ttacaggaac acgaccgctt ccgtcagtgg
    33961 aacaacgaac tggcgggttg gcgtgcgcag ttctcccaac aaaccagcga tcgcgagcat
    34021 ctgcggcaat ggcagcaaca gttaacccat gctgagcaaa aacttaatgc gcttgcggcg
    34081 atcacgttga cgttaaccgc cgatgaagtt gctaccgccc tggcgcaaca tgccgagcaa
    34141 cgcccactgc gtcagcgcct ggtcgcgctg catgggcaga ttgttcccca acaaaaacgt
    34201 ctggcgcagt taatggtcac tatccagaat gtcactctgg aacagacgca acgtaatgtc
    34261 gcacttaacg aaatgcgcca gcgttataaa gaaaagacgc agcaacttgc cgatgtgaaa
    34321 accatttgcg agcaggaagc gcgcatcaaa acgctggaag cccagcgtgc acagttacag
    34381 gcgggtcagc cttgtccact ttgtggttcc accagccacc cggcggtcga ggcgtatcag
    34441 gcgctggagc ctggtgttaa tcagtctcga ttactggcgc tggaaaacga agttaaaaag
    34501 ctcggcgaag aaggtgcggc gctgcgcggg caactggatg cattaacgaa gcagcttcag
    34561 cgcgatgaaa acgaagcgca aagcctccga caagatgagc aagcacttac tcaacaatgg
    34621 caagccgtca cggccagcct caatatcacc ttgcagccac aggacgatat tcaaccgtgg
    34681 ctggatgcac aagatgagca cgaacgccag ctgcggttac tcagccaacg acatgaatta
    34741 caagggcaga ttgccgcgca taatcagcaa attatccagt atcaacagca aattgaacaa
    34801 cgtcagcagc agctcttaac ggcattggcg ggttatgcac tgacattgcc acaggaagat
    34861 gaagaagaga gctggttggc gacacgtcag caagaagcgc agagctggca gcaacgccag
    34921 aacgaattaa ccgcgctgca aaaccgtatt cagcagctga caccgattct ggaaacgttg
    34981 ccgcaaagtg atgatctccc gcacagcgaa gaaactgtgg cgctggataa ctggcggcag
    35041 gttcatgaac aatgtctcgc attacacagc cagcagcaga cgttacagca acaggatgtt
    35101 ctggcggcgc aaagtctgca aaaagcccaa gcgcagtttg acaccgcgct acaggccagc
    35161 gtctttgacg atcagcaggc gttccttgcg gcgctaatgg atgaacaaac actaacgcag
    35221 ctggaacagc tcaagcagaa tctggaaaac cagcgccgtc aggcgcaaac gctggtcact
    35281 cagacagcag aaacgctggc acagcatcaa caacaccgac ctgacgggtt ggctctcact
    35341 gtgacggtgg agcagattca gcaagagtta gcgcaaactc accaaaagtt gcgtgaaaac
    35401 accacgagtc aaggcgagat tcgccagcag ctgaagcagg atgcagataa ccgtcagcaa
    35461 caacaaacct taatgcagca aattgctcaa atgacgcagc aggttgagga ctggggatat
    35521 ctgaattcgc taataggttc caaagagggc gataaattcc gcaagtttgc ccaggggctg
    35581 acgctggata atttagtcca tctcgctaat cagcaactta cccggctgca cgtgcgctat
    35641 ctgttacagc gcaaagccag cgaggcgctg gaagtcgagg ttgttgatac ctggcaggca
    35701 gatgcggtac gcgatacccg taccctttcc ggcggcgaaa gtttcctcgt tagtctggcg
    35761 ctggcgctgg cgctttcgga tctggtcagc cataaaacac gtattgactc gctgttcctt
    35821 gatgaaggtt ttggcacgct ggatagcgaa acgctggata ccgcccttga tgcgctggat
    35881 gccctgaacg ccagtggcaa aaccatcggt gtgattagcc acgtagaagc gatgaaagag
    35941 cgtattccgg tgcagatcaa agtgaaaaag atcaacggcc tgggctacag caaactggaa
    36001 agtacgtttg cagtgaaata actattcagc aggataatga atacagaggg gtgaattatc
    36061 tcttggcctt gctggtcgtt atcctgcaag ctatcacttt attggctacg gtgattggta
    36121 gccgttctgg tggttgtgat ggtggtatga aaaaagtcat tttatctttg gctctgggca
    36181 cgtttggttt ggggatggcc gaatttggca ttatgagcgt gctcacggag ctggcgcata
    36241 acgtaggaat ttcgattcct gctgccgggc atatgatctc gtattatgca ctggtggtgg
    36301 tggtcggtgc gccaatcatc gcactctttt ccagccgcta ctcactcaaa catatcttgt
    36361 tgtttctggt ggcgttgtgc gtcattggca acgccatgtt cacgctctct tcgtcttacc
    36421 tgatgctcgc cattggtcgg ctggtatccg gctttccgca tggcgcattt tttggcgtcg
    36481 gagcgatcgt gttatcaaaa attatcaaac ccggaaaagt caccgccgcc gtggcgggga
    36541 tggtttccgg gatgacagtc gccaatttgc tgggcattcc gctgggaacg tatttaagtc
    36601 aggaatgctg gcgttacacc tttttattga tcgctgtttt taatattgcg gtgatggcat
    36661 cggtctattt ttgggtgcca gatattcgcg acgaggcgaa aggaaatctg cgcgaacaat
    36721 ttcacttttt gcgcagcccg gccccgtggt taattttcgc cgccacgatg tttggcaacg
    36781 caggtgtgtt tgcctggttc agctacgtaa agccatacat gatgtttatt tccggttttt
    36841 cggaaacggc gatgaccttt attatgatgt tagttgggct agggatggtg ctgggaaata
    36901 tgctaagtgg caggatttca ggacgttatt caccactgcg cattgcagca gtgactgact
    36961 ttatcattgt actggcactg ctgatgctct ttttctgcgg cggcatgaaa acaacgtcgc
    37021 ttatttttgc ttttatttgt tgcgcgggat tatttgccct ttcagcacct ctgcaaatat
    37081 tgttactgca aaatgccaaa ggaggagagt tattaggtgc cgcaggtggg caaatagcgt
    37141 ttaacctcgg tagcgccgtc ggcgcatatt gcggaggtat gatgctgacg ctggggctgg
    37201 catataatta cgtggcgctg cctgccgccc tgctttcgtt tgctgcgatg tcatcgttgc
    37261 tgctgtatgg tcgctataag cgccagcaag cggcggatag tccggtgctg gcgaaaccac
    37321 tggggtagat tactgtcacg gtgcgcgttg cttattgccg gatgcggcgt gaacgcctta
    37381 tccgccctac gcggttctgg cacattttgt aggcctgata agacgcggca agcgtcgcat
    37441 caggcatgat gcgccaattg cctacgtttt ttactcttgt ggccataacc acgcagcgcc
    37501 gcgtacgccg ctggaatcac cgtgcttcgc cttactcacc ggcgtttcac attcgccgcc
    37561 gaagacaaat tgtttaatca actgcccaac cgtttgatat aaacggtcta cattgctcat
    37621 cccgcccccc aggacaatca catccggatc gagaatattc acgacatgtg ccagcgattt
    37681 tgccagccgc agctcgtagc gacgcaatgc cagttccgct accggatcgc tttcttcaac
    37741 caggcggata atttcactgc ctttcagcgc atgtccgctc aaacgacgat aatccgtcgc
    37801 gaatcccgtg cccgaaataa aggtttcaat acaaccttgt ttaccgcaat aacaagggac
    37861 ttcctcgcga taacgcagtt cgtcttcgtc catccacggt agcggattgt gtccccactc
    37921 acctgccgtg ccattgccgc cgatatgcgt ccgcccattg aatgccacgc ccgcgccgca
    37981 tcccgtgccg ataatcacgg caaataccgt ctgcgctccc gctgccgcgc catctactgc
    38041 ttctgaaacc gccagacagt tggcatcatt tgccagccgc acttcccgct gcaacctcgc
    38101 gcttaagtct ttatcgaatg gctgaccgtt gagccaggtt gaattgacat tcttcaccac
    38161 accggtgtag ggcgaaatcg aacctggaat gcccatacct accgttccgc gctgccctgt
    38221 cgcctgctcc gccatatcaa ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc
    38281 acgcggcgtg ggcagacgat ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac
    38341 ttcagttttg gtgccgccta aatcgatacc tatacgcact gtactctcct tatttttttc
    38401 aatatcaata gcgtagagac ggacaaccgg attggcaatg caaggccgcc gacaattcgt
    38461 tatcatgccc gctaaattta acgacaaggc cgtggaaatt atcatgctgt ggttcaaaaa
    38521 tttaatggtt taccgtctta gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca
    38581 gctagcctcg atggcattta ccccatgcgg cagccaggac atggcgaaga tgggctgggt
    38641 tcctccgatg ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat
    38701 ctgcgcgcgc aaagaagaaa aaatcctccc gtctccggtg attaaacagg cgctggaagc
    38761 gaaaatcgcc aaactggaag cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc
    38821 gctgaaagac gaagtgctgc actctctgct gccgcgtgct ttcagccgtt ttagccagac
    38881 aatgatgtgg ctcgacacgg ttaacggttt gattatggtg gactgcgcca gtgccaaaaa
    38941 agcggaagat acgctggcat tactgcgtaa aagcctgggg tcgttaccgg ttgtaccatt
    39001 gagcatggaa aacccgattg aactgacgct gaccgaatgg gttcgctccg gtagtgcggc
    39061 acagggcttc cagctgcttg atgaagccga gctgaaatcg ttgctggaag atggcggcgt
    39121 gatccgcgcg aagaaacaag atctgaccag cgaagagatc accaatcaca ttgaagccgg
    39181 aaaagtggtg actaaactgg cgctcgactg gcagcagcgc attcagtttg tgatgtgcga
    39241 cgatggttcg ctcaagcgtc ttaagttctg cgacgagctg cgcgatcaaa acgaagatat
    39301 cgaccgtgaa gatttcgccc agcgttttga tgccgatttc atcctgatga ctggtgaact
    39361 ggcagcgtta attcaaaacc tgattgaagg attaggtggc gaagcacaac gttaattgct
    39421 gattttcctt taatgccgga tgcgacgcct gccgcgtctt atccggcgta cgaagccaca
    39481 ccaggcatat aattattcgc tacggcgagc aataattttt agcgcagcaa tattatgcgt
    39541 tttacgccgt aactgatcta atcgcctcat ccctattcgc ctgtcagcag cacgcagaat
    39601 attcactaac ccccatttat cgctggtaaa ttgttttgtt aacagctccg cttttggggt
    39661 atcaatatat tcacgcaata aatcagaaat tgaacaaata tccgtcaggt aaggatacca
    39721 gcaagtttca ccgtgaaaat tacgcactcc agcgttacct gcaataaagt ctttcgggta
    39781 atcccagatc acgtttttat ccaacgtgat ccaataacgc ggtaaatcgg tacttccacc
    39841 attttggctg cgcatgggat aacgggtaca gtgaatttgc aggttgatcg ttggcgtcag
    39901 taagtcatag atctcccgct gtaacttgct ccatggacgt tgtgtcattg tttttcctca
    39961 agccggtgac gcgcttcact gcgaattttc cgcgttgccc cggttttcac taaatcacgt
    40021 aactgcgaaa gatcgggttg ttcgacaaat aagagtaatg atggatcctc ttttaaccac
    40081 gccgttttca catcggcagc cagctgtgga ttattaatag ccagcgaccg atcctgaggc
    40141 tcagtcaatg tcgttaacaa cgacgccggg gtatgtgcgt tgcgtaatgc cgtttggcgc
    40201 aaatgatcat tgcgactttg cagcaatgca cgccaccatt catcatctga tttatctttt
    40261 atcgtagcca gttccgcaaa aaatacctgg agatcttcac tgatatttat atcaaccttg
    40321 atgaattatg aatatatcgg aaattgaaaa aatcgcagca aaggaaatat tattatgggt
    40381 taaagagagg atatttcaga aaaatgccct cccgttcaag gggaaggcga ggaattacag
    40441 atagcggcac agataagagg taggttcggc aacttgaaga tgaaactcac tgtgaccggg
    40501 aacattaaac accgaaccgg cttcatacac ttgccagtcg gtcgcgtcag gcagtaacac
    40561 attcagcgcg ccactgatta ccgtcatctc ttccggctca gcggtgctga aggtgtattc
    40621 gccttcaacc ataacaccca cgctggcgcg accagtgctg ctgctggaaa agccgatcga
    40681 tttcactttg ccggaaaagt actcattact ttgaagcata aactggccct gtctggtagt
    40741 tgaacatcta cttcactata ggggccagag gcgctggctg tcacgcaaaa ttacaccagt
    40801 aattccgcag ccagccgtgc aatcaatacg ttagagagca agacaggaac atcgagctgt
    40861 ttttgcagta aatcgcgatg acgttggtga aatcctaaac aatccagcat gatgacatca
    40921 gccccttttg ccagtaattc tttcccagca tcaatgattt tttgttctga atcatgaatg
    40981 gggttaccca atgaaaatac cggcgatttc tgcaaaattt gccatttttg cgcctgaacg
    41041 ggcagcatct cctcaaccgg aacgataacc cccacctgat gatcttcaac aatagaggaa
    41101 accagtggag gcaatattcg cgacggctca agaaagatcg tattacgcgc agtcatacta
    41161 ctaatgtttg ctgtactcat taataaaatg acgtcataac cctgattatc gagcacttca
    41221 accacacctt gcagatctcg ctccacttta cgacgcgaaa catgggccag ctggttgtca
    41281 tttaataatg tgagaatggt gtcttcgcct gcttctggcg cgtactccgc catcacttct
    41341 tcacgactta acttccccag caggctatga tgggaaatat tatcttcgtc aatgtattcc
    41401 gtcaggagcg gcaaaacctc ctgcataggt acaatgccga tggtcaggat cgccaacgac
    41461 gcactcattg ttcatccacc ttacttttct ttcgtcgtta attaccgggc aagtgtgaag
    41521 caccactgct gacacttact tgtgctacaa atgacaaaaa gcgtagcagc agacgtgcac
    41581 ggcataatgt aaagattcca aatgattcca gtaatggaat gttattgttt aatattctaa
    41641 ttattagaaa aacataaatt atgaaaaatg tgacgcagat cccttgtagt ggcactcaga
    41701 atcccttccg agcagtctga tgcggtcact ctttgtcagg gtcctcatag cgttttttcg
    41761 catccatcgc ttcctgagcc gtcggatgtt cactgatcaa cgagtctggt ttaggatgat
    41821 cggctctgag ttggtaccag gttaccgtct gtcctggctt tcctttttca atcgtcacta
    41881 tatatgcttc acgaggataa ggcggtttcg ttggcatcgt tcttccttat ttcacgggat
    41941 gaacgttaag tataggcgct ctaaaatcaa caattgatcg tctgtgccag ggcgctacga
    42001 atttcagaaa tcacctggct gggttcgttt gttgcgtcga tgataatatg cgcaacttcg
    42061 cgatatagcg catcgcgttc ttccagcact tcctgaactt cttcgctcag cggttttccc
    42121 gttaaggttg gccgtaaatc ttcttccggt gcagcttgca gtcggttaac caggactgat
    42181 actggcgcac acaaataaac cacgatcccg ttattttgca tgaagtgacg attaaattcc
    42241 gtcagaataa tgccgccgcc tgtagcgata acggtggatg ccgcagttac cgcttccagc
    42301 gccgccgttt ctctggcgcg aaatcccgcc cactcttccc tttcgacgat ctccgcgacc
    42361 gtcatattga gctgtgattg caaccactga tcggtatcga caaaccgacg gttaagcgaa
    42421 tcggcaaggg ccattccgac cgttgtttta ccacagcccc gaggcccgat cagaaaaaga
    42481 ggttgtgtca tcgtgggttt tccccaatag gtcgcaatgc ggcgaaagcc ggtgtcatga
    42541 gaatagcgat cataccatca aactagtaca atttcgattg taaagaaaaa attccactta
    42601 aagtaaaaat ctcaatacat cccttactat accaataaat attcaagaat gaagtgtaaa
    42661 taataaatta catttagcca cgactacgtt gcacttccag ccaccacttc tccagctccg
    42721 cggcaaaggc ctggcggtca cgttgtgaaa ggctatctgg tccgccggtc tggatcccac
    42781 tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg ctcacgaatg gtcgctggcg
    42841 tgtaacgttc gccgcgcgaa ttaagcgccg ctgcgccttt ctcgatggct tcagcagcca
    42901 aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca ctgccggaca atttcgttat
    42961 cggcaacgtc gaaacctgcc gcgacgcgca gcgtacgaat aaatcgcgat ggcggcacgc
    43021 gtaaactctg gtttgctacc agtaccagcg gcatctgcat acgttccgcc gcgcgataca
    43081 aaatctcttt aattacattg ggacacgcgt cggcatccac ccaaattgtc ataaagtcat
    43141 cctttgttag gtaatcctct attgtgtcgc gcttttgcct tccggcatag ttctgtttat
    43201 gcttctgcca gcgattatca aaacaatgaa tttcacggca ggagtgaggc aatggaaaag
    43261 aaaatcggtt ttattggctg cggcaatatg ggaaaagcca ttctcggcgg tctgattgcc
    43321 agcggtcagg tgcttccagg gcaaatctgg gtatacaccc cctccccgga taaagtcgcc
    43381 gccctgcatg accagttcgg catcaacgcc gcagaatcgg cgcaagaagt ggcgcaaatc
    43441 gccgacatca tttttgctgc cgttaaacct ggcatcatga ttaaagtgct tagcgaaatc
    43501 acctccagcc tgaataaaga ctctctggtc gtttctattg ctgcaggtgt cacgctcgac
    43561 cagcttgccc gcgcgctggg ccatgaccgg aaaattatcc gcgccatgcc gaacactccc
    43621 gcgctggtta atgccgggat gacctccgta acgccaaacg cgctggtaac cccagaagat
    43681 accgctgatg tgctgaatat tttccgctgc tttggcgaag cggaagtaat tgctgagccg
    43741 atgatccacc cggtggtcgg tgtgagcggt tcttcgccag cctacgtatt tatgtttatc
    43801 gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac gcgcccaggc gtataaattt
    43861 gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg aaacgggaga acatccgggg
    43921 gcactgaaag atatggtctg ctcaccggga ggcaccacca ttgaagcggt acgcgtactg
    43981 gaagagaaag gcttccgtgc cgcagtgatc gaagcgatga cgaagtgtat ggaaaaatca
    44041 gaaaaactca gcaaatcctg atgactttcg ccggacgtca ggccgccact tcggtgcggt
    44101 tacgtccggc tttctttgct ttgtaaagcg ccaaatctgc cgatttcaac cactcacgat
    44161 agtgactcat ttgtgggttc agcggcgcaa cccccacact aatccgtaaa gttacctgtg
    44221 gcgtattcgg caagcgtaac gtgtttagcc cttcacgcac ccgtaacatg gcggtaatgg
    44281 cgctctcagc tggcgtaccg gacatgatta ctgcaaactc atcgccgcca aaccgaccaa
    44341 tcacatcgct accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat
    44401 cgcccacatc atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca
    44461 gtaacgttgc atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt
    44521 cccaatgacg tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc
    44581 tgcgtttatg ttccgccagt ttggttgccg tctggtagct gacccagcca aacatcagag
    44641 gataaacgac aataatggga agggagagcc accattccag cggagcacta ttgaacgaca
    44701 cggtaatgcc cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg
    44761 cgacaaacag acgggggccg cctgccccca tcaaattcag acacagaatc atcaacatag
    44821 cggtggaagg cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat
    44881 cggtttttaa gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc
    44941 aggctaaatg cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg
    45001 gcgggtgcga aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac
    45061 ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag
    45121 ttaaaggagg ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta
    45181 tttttgggaa cattcagaaa caattttccc aaattataga gacggggcta aaggcag
//
LOCUS       DAJTYH010000021        43479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTYH010000021 DAJTYH010000000
VERSION     DAJTYH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="OO609_001166"
     CDS             complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="OO609_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6632662.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1474..2898
                     /gene="miaB"
                     /locus_tag="OO609_001167"
     CDS             1474..2898
                     /gene="miaB"
                     /locus_tag="OO609_001167"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6632663.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3051..4091
                     /locus_tag="OO609_001168"
     CDS             3051..4091
                     /locus_tag="OO609_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6632664.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4088..4555
                     /gene="ybeY"
                     /locus_tag="OO609_001169"
     CDS             4088..4555
                     /gene="ybeY"
                     /locus_tag="OO609_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6632665.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4645..5523
                     /gene="corC"
                     /locus_tag="OO609_001170"
     CDS             4645..5523
                     /gene="corC"
                     /locus_tag="OO609_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6632666.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5548..7086
                     /gene="lnt"
                     /locus_tag="OO609_001171"
     CDS             5548..7086
                     /gene="lnt"
                     /locus_tag="OO609_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6632667.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7483..8391
                     /gene="gltI"
                     /locus_tag="OO609_001172"
     CDS             7483..8391
                     /gene="gltI"
                     /locus_tag="OO609_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6632668.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8561..9301
                     /gene="gltJ"
                     /locus_tag="OO609_001173"
     CDS             8561..9301
                     /gene="gltJ"
                     /locus_tag="OO609_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6632669.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9301..9975
                     /gene="gltK"
                     /locus_tag="OO609_001174"
     CDS             9301..9975
                     /gene="gltK"
                     /locus_tag="OO609_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6632670.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            9975..10700
                     /gene="gltL"
                     /locus_tag="OO609_001175"
     CDS             9975..10700
                     /gene="gltL"
                     /locus_tag="OO609_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6632671.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            10818..11753
                     /gene="rihA"
                     /locus_tag="OO609_001176"
     CDS             10818..11753
                     /gene="rihA"
                     /locus_tag="OO609_001176"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6632672.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            11837..13507
                     /gene="hscC"
                     /locus_tag="OO609_001177"
                     /pseudo
     CDS             11837..13507
                     /gene="hscC"
                     /locus_tag="OO609_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13567..15018)
                     /gene="djlC"
                     /locus_tag="OO609_001178"
     CDS             complement(13567..15018)
                     /gene="djlC"
                     /locus_tag="OO609_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6632673.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15015..15722)
                     /locus_tag="OO609_001179"
     CDS             complement(15015..15722)
                     /locus_tag="OO609_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6632674.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            15824..16378
                     /locus_tag="OO609_001180"
     CDS             15824..16378
                     /locus_tag="OO609_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6632675.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16388..17815)
                     /locus_tag="OO609_001181"
     CDS             complement(16388..17815)
                     /locus_tag="OO609_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6632676.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(17812..18519)
                     /locus_tag="OO609_001182"
     CDS             complement(17812..18519)
                     /locus_tag="OO609_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6632677.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            18683..19660
                     /locus_tag="OO609_001183"
     CDS             18683..19660
                     /locus_tag="OO609_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6632678.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(19730..20212)
                     /locus_tag="OO609_001184"
     CDS             complement(19730..20212)
                     /locus_tag="OO609_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6632679.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20447..23029
                     /gene="leuS"
                     /locus_tag="OO609_001185"
     CDS             20447..23029
                     /gene="leuS"
                     /locus_tag="OO609_001185"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6632680.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23044..23625
                     /gene="lptE"
                     /locus_tag="OO609_001186"
     CDS             23044..23625
                     /gene="lptE"
                     /locus_tag="OO609_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6632681.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23625..24656
                     /gene="holA"
                     /locus_tag="OO609_001187"
     CDS             23625..24656
                     /gene="holA"
                     /locus_tag="OO609_001187"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6632682.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24658..25299
                     /gene="nadD"
                     /locus_tag="OO609_001188"
     CDS             24658..25299
                     /gene="nadD"
                     /locus_tag="OO609_001188"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6632683.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25323..25934
                     /locus_tag="OO609_001189"
     CDS             25323..25934
                     /locus_tag="OO609_001189"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6632684.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26194..26511
                     /gene="rsfS"
                     /locus_tag="OO609_001190"
                     /gene_synonym="rsfA"
     CDS             26194..26511
                     /gene="rsfS"
                     /locus_tag="OO609_001190"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6632685.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26515..26982
                     /gene="rlmH"
                     /locus_tag="OO609_001191"
     CDS             26515..26982
                     /gene="rlmH"
                     /locus_tag="OO609_001191"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6632686.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27013..28914
                     /gene="mrdA"
                     /locus_tag="OO609_001192"
     CDS             27013..28914
                     /gene="mrdA"
                     /locus_tag="OO609_001192"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6632687.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            28917..30029
                     /gene="mrdB"
                     /locus_tag="OO609_001193"
                     /gene_synonym="rodA"
     CDS             28917..30029
                     /gene="mrdB"
                     /locus_tag="OO609_001193"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6632688.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30040..31128
                     /gene="rlpA"
                     /locus_tag="OO609_001194"
     CDS             30040..31128
                     /gene="rlpA"
                     /locus_tag="OO609_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6632689.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31267..32478
                     /gene="dacA"
                     /locus_tag="OO609_001195"
     CDS             31267..32478
                     /gene="dacA"
                     /locus_tag="OO609_001195"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6632690.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32589..32852
                     /locus_tag="OO609_001196"
     CDS             32589..32852
                     /locus_tag="OO609_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6632691.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            32953..33594
                     /gene="lipB"
                     /locus_tag="OO609_001197"
     CDS             32953..33594
                     /gene="lipB"
                     /locus_tag="OO609_001197"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6632692.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            33853..34806
                     /locus_tag="OO609_001198"
     CDS             33853..34806
                     /locus_tag="OO609_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6632693.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35015..35980
                     /gene="lipA"
                     /locus_tag="OO609_001199"
     CDS             35015..35980
                     /gene="lipA"
                     /locus_tag="OO609_001199"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6632694.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36081..36284)
                     /gene="tatE"
                     /locus_tag="OO609_001200"
     CDS             complement(36081..36284)
                     /gene="tatE"
                     /locus_tag="OO609_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6632695.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36413..37201)
                     /locus_tag="OO609_001201"
     CDS             complement(36413..37201)
                     /locus_tag="OO609_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6632696.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37294..37677
                     /gene="crcB"
                     /locus_tag="OO609_001202"
     CDS             37294..37677
                     /gene="crcB"
                     /locus_tag="OO609_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6632697.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(37731..37940)
                     /gene="cspE"
                     /locus_tag="OO609_001203"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(37731..37940)
                     /gene="cspE"
                     /locus_tag="OO609_001203"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6632698.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38115..38675)
                     /gene="pagP"
                     /locus_tag="OO609_001204"
     CDS             complement(38115..38675)
                     /gene="pagP"
                     /locus_tag="OO609_001204"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6632699.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39264..40649
                     /gene="dcuC"
                     /locus_tag="OO609_001205"
     CDS             39264..40649
                     /gene="dcuC"
                     /locus_tag="OO609_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6632700.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40690..41031)
                     /locus_tag="OO609_001206"
                     /pseudo
     CDS             complement(40690..41031)
                     /locus_tag="OO609_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(41369..41878)
                     /locus_tag="OO609_001207"
     CDS             complement(41369..41878)
                     /locus_tag="OO609_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6632701.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            42041..42286
                     /locus_tag="OO609_001208"
     CDS             42041..42286
                     /locus_tag="OO609_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6632702.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            42261..>43479
                     /locus_tag="OO609_001209"
     CDS             42261..>43479
                     /locus_tag="OO609_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6632703.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKH"
BASE COUNT        10933 a        10881 c        10838 g        10827 t
ORIGIN      
        1 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
       61 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
      121 acggctggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
      181 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
      241 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
      301 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
      361 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
      421 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
      481 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
      541 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
      601 gccatattca tattctgcaa ctggcgaata cgcgtcggag agtcatacca caccagcgat
      661 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
      721 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatgcacga ctgcgcatac
      781 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gagaatttgc gccgtcggca
      841 ccaatcacca gtttcgcgcg gatcgtttca ccgcccttca gttccagctc ctgaagatca
      901 ttatctcgat gtaatgcaat cagcgaggtt ggcacacgta acgttacttt cggatgcgct
      961 tccagcgcct gccacaacgc ctgttgcagg actgtgtttt ccaccatgta gccaagcaac
     1021 ggcaacttta attcggctgc gtcaaatacc acatgtgccg tttcccactc ccaggtttcc
     1081 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
     1141 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
     1201 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
     1261 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
     1321 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
     1381 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
     1441 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
     1501 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
     1561 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctactgct gaacacctgc
     1621 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
     1681 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
     1741 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
     1801 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
     1861 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
     1921 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
     1981 cgtggtgaag aggtaagccg tccgtctgac gatattctgt ttgagattgc ccagcttgcg
     2041 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
     2101 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
     2161 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
     2221 gaagtgtacc gcgacacgcc tgagctggtg agcttcctgc atctgccggt acagagcggt
     2281 tccgatcgca ttctgaacct gatgggacgt acccatacgg cgctggagta caaagcgatc
     2341 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
     2401 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
     2461 ttcgacatga gctacagctt tatcttctct gcacgtccag gaacgcctgc cgccgatatg
     2521 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agaacgtatc
     2581 aatcagcaag cgatggcatg gagccgtcgg atgctcggca ccacccagcg tattctggta
     2641 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
     2701 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
     2761 gtctacccga actctctgcg cggtaaagtg gtgcgaactg aagatgaaat gggtctgcgc
     2821 atggcagaaa caccggagtc ggtgattgcc cgcacccgca aagaaaacga ccttggcgtg
     2881 ggttattatc agccgtaatt ctcaggcctg ccgttccggt gggccttgtt ttttctctct
     2941 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
     3001 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
     3061 acactcgcga aatcaccctg gagccagcag acaatgcgcg tctgttgagc ctgtgcggcc
     3121 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
     3181 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
     3241 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
     3301 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
     3361 agtacggcaa agcggtcaat atcaaaacta aacgcggcgt aattaagccg cgcacgccaa
     3421 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
     3481 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
     3541 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
     3601 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccactgtac gacgcgctgt
     3661 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
     3721 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
     3781 gccagaacac taccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
     3841 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
     3901 tacgtcatgc cattgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
     3961 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcttat gaagcctggg
     4021 aagaagcaga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
     4081 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
     4141 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
     4201 gtcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
     4261 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
     4321 ggagatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
     4381 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
     4441 tttgttaggt tacgatcaca ttgaagatga cgaagcagaa gaaatggaag ccctcgaaac
     4501 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
     4561 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
     4621 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
     4681 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
     4741 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
     4801 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
     4861 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
     4921 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
     4981 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
     5041 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
     5101 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
     5161 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
     5221 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
     5281 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
     5341 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
     5401 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
     5461 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
     5521 taaaaccgaa actggataga taactacatg gattttgcct cattaattga acgccagcgc
     5581 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
     5641 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgt
     5701 cgcccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
     5761 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
     5821 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
     5881 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccac ccctgctctc
     5941 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
     6001 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgctaatggg cgtggaagcc
     6061 attaacttcc tgctgatgat ggttagtggc ctgctggcac tggcgttggt caaacgcaac
     6121 tggcgtccgc tggtggtggc cgtcgtgctg tttgccctac ccttcccgct gcgttacatc
     6181 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
     6241 ccgcaatcgc tgaaatggga cgaaggccag cttcttaata cgctaaagat ttactacaac
     6301 gcaacggcac cgctgatggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
     6361 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
     6421 ggtagctcgc tggtaaccgg gattgtcgac gcgcgtctca ataagcagaa ccgctacgat
     6481 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
     6541 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
     6601 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
     6661 cagccgccgc tgtcggcaaa tggtattgag cttactgcgg ctatttgcta cgagattatt
     6721 ctcgccgagc aagtgcgcga taacttccgc ccggataccg actacctgct gactatctcc
     6781 aacgatgcgt ggtttggtaa atctattggt ccatggcaac acttccagat ggcgcgaatg
     6841 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
     6901 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcgcccgcga ggtgttaacc
     6961 actaacgtga cgccgaccac cggactcaca ccatacgcac gtaccggcaa ctggccgctg
     7021 tgggtgctga cggcactgtt tggttttgct gctgtgttga tgagtctgcg tcagcgacgt
     7081 aaataatccc ttcttatcgt gccggaatac atcccatatt tcggcacgct caccggatgc
     7141 ggcatcagtg cattatccgg ataacaatat tctcctatcc aactttggca catctattgc
     7201 tttgttatac aaggcaaagc ttgaaccagc atcagtacaa cccggttgca ccacagcaat
     7261 ccaccggtag caccaaaaga gtgcatttta agaattttgc ttcagaatgg tgcggtgcac
     7321 ctcgcaaaaa caaacaataa aacatcaata tctttacatt tagccaaact aaatgttacc
     7381 taacaggcac aacactgcac aataaagttg cagacgataa caacacaaac actcacaacg
     7441 ggtatccatg cgttcttaac gcagaagata aaggtgttgg atatgcaatt acgtaaacct
     7501 gccacagcaa tcctcgccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
     7561 ccggcagcag gcagtacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
     7621 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggttactcg
     7681 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
     7741 caggtaaaac tgattccgat tacctcacaa aatcgtattc cactgctgca aaacggcact
     7801 ttcgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
     7861 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggcgg cgatatcaaa
     7921 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcactac ctctgaagtt
     7981 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
     8041 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
     8101 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga tatcgtcggc
     8161 aagccgcagt ctcaggaagc ctacggttgc atgttgcgta aagacgatcc gcagttcaaa
     8221 aagctgatgg atgacactat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
     8281 gataaatggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
     8341 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
     8401 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
     8461 atggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
     8521 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
     8581 gggtattttt ttacaacaag ccccgttcgg caataccacc tatcttggtt gggtctggag
     8641 tggttttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
     8701 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
     8761 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtatctggt
     8821 gatcccggag ctgctgccgg agaaaatcgg catgtggttt aaggccgagc tggatcccaa
     8881 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
     8941 cgaacaggta cgcgccgcta ttcagtcgct accgcgtggg cagaagaatg ccgcgctggc
     9001 gatgggcctg acgctaccgc agacttatcg atacgtactg ctgcctaatg cttatcgcgt
     9061 tatcgtcccg ccgatgactt ccgagatgat gaacctggtg aaaaactccg ccatcgcctc
     9121 gaccatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgact actcggctca
     9181 cgcctgggag tcgtttaccg ccatcacgct tgcgtatgtg ttgattaacg cttttatcat
     9241 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
     9301 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
     9361 gtgatcaccc tgaaaatcac cgtcacggca gtcgtgattg gtattttgtg gggtaccatg
     9421 ctggcggtga tgcgcttgtc cagcttcgcg cctgtttcgt ggtttgccaa agcctacgtt
     9481 aacgtattcc gctcgattcc tttagtcatg gttttgctgt ggttttactt gattgtgccg
     9541 ggatttctgc aaaatgtgct gggattatcg ccaaaaaatg atattcgttt aatttcggcg
     9601 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgccggtatt
     9661 caaagtattt cccgtggtca gtcgagcgcc gcgctggcgt tggggatgac tcactggcag
     9721 tcgatgaaac tgattattct gccgcaggct ttccgcgcga tggtgccgct gctgctcact
     9781 cagggcatcg tactgttcca ggatacctcg ctggtgtatg tgttatctct ggccgatttc
     9841 ttccgtaccg cctcaaccat tggtgagcgt gatggtacgc aggtcgagat gatcctgttt
     9901 gccggatttg tttatttcgt tattagcctt agcgcgtcgt tgttggtcag ctacttgaaa
     9961 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    10021 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt ggtggtggtt tgcggcccgt
    10081 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaacca gtgcagcaag
    10141 gtgaaatcac cgtcgatggt atcgtggtta acgacaagaa aaccgatctg gcaaagctgc
    10201 gctcccgcgt cgggatggta ttccagcatt tcgagctgtt ccctcatctg tcgattatcg
    10261 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcga taaagcgccc gctcgtgaaa
    10321 aagcattgaa attgctggag cgcgtgggac tttccgcaca tgccaataaa tttccggcgc
    10381 agctttccgg cggtcagcag cagcgcgtgg caatcgctcg cgcattgtgt atggctccta
    10441 ttgcgatgct gttcgacgaa ccgacatcgg cgctggatcc ggagatgatc aacgaagtgc
    10501 tggacgtgat ggtggaactg gcgaacgaag gaatgaccat gatggtggtg acccacgaaa
    10561 tgggctttgc ccgcaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    10621 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    10681 tcgcgaaaat cctgcattaa tcactctggc gcacataccc aattgcgcgc catctcacgc
    10741 tttatcgttt cgcccctcgt atctgttaca tcctggcgtt acccttgtcg caaagaagca
    10801 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgatg
    10861 acgctatcgc aatagttctc gcactcgcct caccagagct tgatgtcaaa gcaattacgt
    10921 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    10981 tgcttaatcg caccgatatt ccggtggcag gcggcgcggt aaaaccgtta atgcgtgagt
    11041 tgattatcgc ggacaatgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    11101 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgcgaaa
    11161 gtgcggaacc tgtcacgatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    11221 atagccatcc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    11281 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    11341 aaattgtctt ccagtcaggg atcccggtgg tgatggccgg tctggatgtt actcataaag
    11401 cgcaaatcca cgttgaagac accgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    11461 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    11521 tcggcgcacc gctgcatgac ccatgcacca tcgcctggct gttgaaaccc gagttgttta
    11581 ccactgttga gcgctgggtt ggcgtggaaa cacaggggaa atatacccag ggtatgacgg
    11641 ttgttgatta ttattatctg actggcaata aaccgaatgc cactgtaatt gtcgatgttg
    11701 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taacactcgt
    11761 cattgattaa gatcaccacg cgttacctgg gtggtcttaa tctatttatc tctttttctt
    11821 tcaagatata aatgcaatgg ataatgcaga actcgccata ggtatcgatc tcggcactac
    11881 caatagttta attgccgtct ggaaagacgg tgccgcgcaa ttaattccaa ataagttcgg
    11941 tgaatattta acaccatcca taattagcat ggatgaaaat aatcatattt tagtcggaaa
    12001 accggctgta tcacggcgta cttcgcatcc ggataaaacg gcagcgttat ttaaacgtgc
    12061 aatgggcagt aataccaact ggcggttagg cagcgacaca tttaacgcgc cagaactgtc
    12121 ctctttggta ttacgctcat taaaagaaga tgccgaagaa tttctgcaac gtccgattaa
    12181 agatgtggtg atctccgttc tggcttattt cagcgatgaa caacgcaagc atacccgttt
    12241 agcagcggac ttagccgggt taaatgcggt acgcttaatt aatgaaccca cagcagctgc
    12301 gatggcgtat ggcctgcata cccaacaaaa tacccgttcg ctggtgtttg atctcggtgg
    12361 cggcacgttt gacgttacgg tgcttgagta cgccacgccg gtgattgaag ttcacgcctc
    12421 cgctggcgac aacttcctcg gtggcgaaga ttttacccat atgttggtcg atgacgtttt
    12481 aaagcgcgcg gatgtcacaa ggaccacgct taacgagagt gaactggcag ccttgtacgc
    12541 ctgtgtggaa gcggcaaaat gtagcaatca atcgccaatg cacattcgct ggtagtacca
    12601 ggatgaaacg cgggaatgcg aattttacga gaacgaactg gaagatttgt ggttgccgct
    12661 gctcaatcgc ttgcgagtgc cgattgaaca ggcgttgcgc gatgcgcgtc tgaagccgag
    12721 tcaaatcgac agtctggtgc tggttggcgg cgcgtcacaa atgccgctgg tgcagcgaat
    12781 cgccgtgcgt ctgtttggca aattaccgta tcaaagttac gatccgagca ccattgtcgc
    12841 gctgggcgca gcaatccagg ccgcctgccg cttacgcagt gaagatattg aagaagtgat
    12901 cctcactgat atttgccctt actcgttggg cgttgaggtt aaccgccagg gcgtttccgg
    12961 cattttctcg ccgattattg aacgaaacac cactgtgccc gtgtcgcgtg tagaaactta
    13021 ttcaaccatg cacccggaac aggattcaat tacggttaac gtctatcagg gagaaaacca
    13081 caaagttaaa aacaacattc tggtggaatc cttcgatgtg ccgttgaaga aaaccggggc
    13141 ttatcagtcg attgatattc gctttagtta tgatatcaac gggttgcttg aagttgacgt
    13201 gcttctggaa gacggcagcg ttaagtccag agtgattaac cacagcccgg taacattgag
    13261 cgcgcagcag attgaagaga gtcggacgcg gttatccgca ttgaaaattt atccgcgcga
    13321 tatgctcatc aatcgcacct ttaaagccaa actggaagag ttgtgggcgc gggcgctggg
    13381 tgacgagcga gaagagattg gccgggtgat caccgatttt gatgcggcgc tgcagtcaaa
    13441 cgatatggcc cgcgtcgatg aggtcaggcg gcgggcgagc gtttatttag ccattgagac
    13501 ctcataatca cggctgccgg atgcggcgta aatgctgcat ccggcatcgg cgcaaaaaac
    13561 acatcgtcaa ctaaacaaca tcgcgatttt gccgagagca taacctataa ccagcaccgt
    13621 taaactgatt ttgataatgc tggtaatgct ctgtggtttt tcaccctcat catttttttc
    13681 ttcatggttt tcaggtgttg gctcatcgac atcttcatcc tccggctcga gttttacttt
    13741 cgtcaacagc atccgggtga taatggcctc taatggatga gcatctccgc cttccagcct
    13801 tgtattaaga cgggccagca tctcttgttt cggaaacgct tcaagtacta cctgttccgc
    13861 tacccgatac aaatccagta acggtgaatc ttcttctgtc tcgctaccga ctctcgattt
    13921 ccaccacgcc catgctttgc gtttgaagaa taacgactca ataaacgatt gacctaacgg
    13981 cgataatttt ccgctatgta aaatttccgc ccataacagc cgcgtccggg cgctctcacc
    14041 aggaacatat ttcagcggtt gaccttgcgc atctaccatg ctccgcgctt cgataaccat
    14101 cactaataac gcgaaataat ccgggcaatg acgcttgctc cactgcaaca acaaattatc
    14161 agcttgtgta gatgggtaac tttcccagta cgcgccgaga tcagcaagca ggctttcccc
    14221 ctcgccgcag tacaaacgtt gtgcacactg gtaataccgg gcatcttcgc tttctggttc
    14281 ttccgcttgc cactgtttgg ctacaggaat gagttccacc atacctgttt gcaccgagct
    14341 gtaccagcgc aacagtttgc gatgtaactt tgcatcatca ggaatgaacc acggtccttc
    14401 catcgccaaa aacgcagtga catagtcagg atggtaacgg cagcaacgtt ccagcgcgaa
    14461 gtaatattca acagtctggt tctgtaacgc gacgggatat tccagaaggc taaggaaatc
    14521 gaaacaatca ccagcctgaa tggcctcaag aaaagcctcc ctttcttcct catccattcc
    14581 ttcgctgtca ttaaacgaat gccagttcaa acgctctgcc aacaaagaag cacaatttaa
    14641 tgacagatat cgcgcttcta tagcgattgc acacagcggc cagcgtaatt gatcgacatc
    14701 ctccatgttc caggtattta attgctgaat aaatttctgc caggcggagg gctgaaaacg
    14761 atcactttct gaatccagta atgtcctgaa tgcacgtagt atttcatgtt cagcggtggc
    14821 atctttttct tcatcatctg cttcctcgac aggatttacc gccagtcgca gcgcctcttc
    14881 ataggcctgg cgtaatgctt taaacccctg cggatcggtt tcgggatgac acaacggtaa
    14941 gcgagcaaga taagcctggc ggataatgtc tatttgcgtc gtgctttcaa tttcgaggat
    15001 ttgccaacat gttttcatag gtcgctcaca tccttaagcg ttaccgggca ttcaggctgg
    15061 acagttaatg gctgccaggg aagcgtggca tagaaacggt cgcttcccgc tgccagctct
    15121 tcgaacatcc gactcccggc gctatcgtat ttgtacaggg ttagcgcctc gcgtagcgaa
    15181 gcgttgtctt cgcactgaga agattgccag tagagacgcc ctagtgaata ggcagaaaaa
    15241 tagtgcatcc agctgtgata gtaatggtga gctcgtgcat agacgcgcga ttgcagccat
    15301 aaactctctt cttccgtcag ccagttgttg agcacaccgg cccgtgacag aaatcccatt
    15361 cgaacataat cccaggcttt gattccccct tctccacagc acatagcggt ttgggcaacg
    15421 aattgggtat aaatcaaccc tctctccgag ccaccagcaa ttaatgcttt ccactcttca
    15481 ggggaagagc gttgccatgt cagatataag ccagctaaat cattggcatg accatcatca
    15541 gtcatctgat aaatagtctg aattaaatca tcacgattat ttattcccca ggatgatgag
    15601 agatcgaatt cttttatccc ggtatacatt tttggcgagt catgcgcggg tttgcaactc
    15661 tgattaagta tttccatggg cgcagaaagc gcaaaaagaa gatattgttc ttccttattc
    15721 attttttaat aatcccttaa gtgcattaac tgactacttt tttattattg atgagtataa
    15781 acttgacgct ttcaaaataa aaagaaaatc gaagcattca cacatgaata aaaaattaat
    15841 gtatatattc gcaattttta tagttgcagc aattacctgt attagccaac ccaagaaaac
    15901 gatgttgcgt gataaagcca tggtgaatta tgcctttgat tatttaagat caccaggcag
    15961 tctaccgtta accacggcag ccacggagct ttccgcgatt catggtcact caacatcgca
    16021 atatcgcctt ggcgaatttt atcttcatgg tagcgacggt aaaccactgg attatacaca
    16081 ggcgagatac tggtatgagc aatcagcaga acaggaaaat ccgcgcgcac aaagcaaact
    16141 ggggtggatc tacctgaaag gcctgggagt caaacccgat acccgtaaag caattctctg
    16201 gtataaggaa gcagctgaac aagggtatgc tcatgctcaa tatactttgg gtttgatcta
    16261 cagaaatggc actggtatta atgttaacca ttatgaatct caaaaatggt taaaactggc
    16321 cgccaaacaa cattacaaaa atgcggaaag attacttgcc ggacttcccg cacattaacg
    16381 tcgactgtca cccgaagaga tggagtattt tccctatcaa accggcaaag ataaaaatgt
    16441 agaaaataat tttgatgacg cccagacagc ccagcccgcc atcatcaacg ggcattggct
    16501 gcggttgcgg ctccggctcg acggtttcct gaccggtaaa attcgccgcc tgaattaata
    16561 atcgacgaat aattaatgct tccagtggac aacgatcttc tgcatcaagg cggctcacaa
    16621 tcggcattaa tgattcttct gtcggttggc tggcctgcac taattgtttg gcaactaaat
    16681 ataatggcca taagggatgt tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt
    16741 tgtgcaaaac catctcgacc agtgaacgcc ccagcgcaga gagtttttca tcgctgagta
    16801 tttcgtataa atgaaaacgc gtacgggcgc tgtcgccggg gacataaagt agcggttttc
    16861 cctgatcgtt aaccagatca cgcgcttcaa tcatcatcac cactaatggg taataataga
    16921 cccgatgctg acggcaccat tgcaacatta aagcatccgc ctgggtggag ggatactcgc
    16981 gccagtagta acaaagttct gccagcaggc tttcgccttc tccacaatag acgcgttgtg
    17041 cgtattcata ataacgcgca tcttcattat caggttcagc ttcccgccac gaccgggcgt
    17101 aatccaggag ttcggggatt cccaggcggc ttgcgctata ccagcggagt aaatttctgt
    17161 gcagttttgc atcatcgggg ataatcactg cgcgatgttg cgctaaatac aatgtcagcc
    17221 attctggatg cgatgaccaa atccgagcga acatttggta aaaatcgatg gtgtcatttt
    17281 gcacggcaac tggcagatgc agaatggtct ggaagttaaa aacattcccc ttcgcaatgg
    17341 catataaaaa ggattccagt tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc
    17401 accgcaatct ttctgctaat aacaccacgc actcgaagga taaatgggca gtgttcatgg
    17461 cgattgtgca cagcgaccag cgtaattcat caatctcttc catcgagcaa taatttaatt
    17521 gctgaatgaa tcgctgccag gcggagggca gaaaacgttc gctatcagag gcaagtaacg
    17581 cgcgaaaggc gagcagaatt tcatgttctg ctacctcata ttcttctggt tgccaaacag
    17641 atttagcagg cgactgcgca atccgtagcg cctcctcata cgcctgacga agttgtttaa
    17701 aaccctgcgg atcggtttct ggatgaaagg acggtaacag cgccagataa gcgcggcgga
    17761 taatatcgac gtcagtcgtt tcctctatat cgaggatctt ccagcaattt ttcataagtc
    17821 gctcatatcc ttaagcgttt ccgggcatgt ggggtagtca gccagtggtt gccaggacaa
    17881 ggtagggtaa tacgcatcat cactggcaaa taattgataa aacatatcat tgccggaatc
    17941 gtcgtattct ttacgcgcta agaattcgcg taataaaggc agatgatcat cttcgggaga
    18001 ctgccaatat tgccgaccaa aggtataacc ggcaaagtat tgccgccagt tactataata
    18061 acgtagagcc cgtagatgaa tacgcgactg gatccataga ctttcttctt cactcaacca
    18121 gttattaagt acgcccattc gacttaaaaa acccatccgg acataatccc atgctttaat
    18181 tcctccctca ccacagcatt cggctgtgct ggcgacaaat tgcgcataag cctgaccttg
    18241 ctcatttaat tcagcaagat agtcacgcca ttcacatgga gaataacgaa accagcggtg
    18301 ataaaaccct gccaaccgag cggcatgacc gttctcgatc attcggtgaa ttatgcgcag
    18361 caggtcatca cggtcatcaa ttccccatga ggaagaaagg tcaaaaaagc atgtacctaa
    18421 atacattttg ggggatgaat gcgaaggtaa acagcattca ttgcgtattt ccatcggagt
    18481 ggaaagtgca aacaatattt tttgcgaccc catgtccata taatttatcc ttaaacattc
    18541 atttaatgaa tgtaaactac atttcgaggc tcagttattt atacttttct gacgcaacat
    18601 aatacgcata aaataaggct tacgcaaaaa tggaacacat aaaaatataa tatattttat
    18661 ctacaaccat aaggaaatga taatgatttt tacgtcaagt tgctgcgata atttatcaat
    18721 agatgagatt atcgaacgtg ctgaaaaagg cgattgcgag gctcaatata ttgttgggtt
    18781 ttattataat cgcgatagcg caattgactc tccggacgac gaaaaggcct tttactggct
    18841 gaagctggcc gctgagcaag gtcattgtga agcacagtat tccttagggc aaaagtatac
    18901 cgaggataaa agccgtcata aagataatga gcaagccatc ttctggctga aaaaagctgc
    18961 cctacaaggc catactttcg cttccaacgc ccttggctgg atactggatc gtggagaaga
    19021 ccccaactat aaagaagcgg ttacctggta tcagatagcc gcggagagcg gaatgtctta
    19081 tgcgcaaaat aatcttggat ggatgtacag aaatggcaac ggagtcgcac aagactatac
    19141 gctggcattt ttctggtaca aacaagctgc attacaaggc catagttacg cacaagacaa
    19201 tctggccgat ctttataaag acggagaagg cgttgctcaa aacaagacac tcgccgcatt
    19261 ctggtatttg aaaagcgcac agcagggtaa tcggcacgcc cagtttcaaa ttgcatggga
    19321 ttataacgct ggcgaagggg tggaccagga ctataagcaa gcgatgtact ggtatctgaa
    19381 ggctgccgct caggaaagcg tcggcgctta cgtcaacatc ggttatatgt ataaacacgg
    19441 acaaggcgtt gagaaagatt atcaggctgc ctttgaatgg tttacgaaag ccgctgaatg
    19501 caatgacgcc actgcctggt ataacctggc cattatgtat cactacggag aaggaagacc
    19561 tgtcgatctc cgacaggctc tcgacctgta tcgtaaagtt cagtcatccg gaaccagaga
    19621 tgtcagtcag gaaattcgtg agactgaagc tttactgtag aaataaaaac aagggccggg
    19681 gaatttcatt ctgcccatat tctctttatt gctgtgcgaa atcagactat tacggctcaa
    19741 acgggcgtct ctggaactgg tcataaccac atttcgggca tagcgtcagc acttccggtg
    19801 tgtagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc ccgaccactt
    19861 ctccgctgtg ataaacccca tgatgattga ggtcctggaa aatttcgcgc cattcaagct
    19921 gcgttttatc ggtgatgtct gccagctcct gccacaagct ttctttaatc acccgcataa
    19981 agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac tcttccaggt
    20041 cacgtctgac agctcgcgtc agctcatcga tctcggttcg cgttaactcc cctgttttta
    20101 ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc
    20161 gttcgttcag tgacgcgacc agttcacggt aatattgagc aaccttgttc atcgtttcat
    20221 ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct ttgctaatac
    20281 gcccacaaac tctgtaaatt aatgtacgaa taatccgggc atcatttcaa cgtcgagtgc
    20341 gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga aaaaccacct
    20401 caacgctaca tttgtagccg tattgaaaac aggaccactg gctgccatgc aagagcaata
    20461 ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc gcacatttga
    20521 agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc cctatccttc
    20581 tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga tcgcccgtta
    20641 ccagcgcatg ctgggcaaaa acgttctgca gccgatcggc tgggacgcgt ttggtctgcc
    20701 tgcggaaggc gcggcggtga aaaacaacac cgctccggca ccgtggacgt acgacaacat
    20761 cgcgtatatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga gccgcgagct
    20821 ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg agctgtataa
    20881 aaaaggcctg gtatataaaa agacttctgc ggtcaactgg tgcccgaacg accagaccgt
    20941 actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca aagttgaacg
    21001 taaagagatc ccgcagtggt ttatcaaaat cactgcttac gctgacgagc tgctcaacga
    21061 tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta actggatcgg
    21121 tcgttccgaa ggcgtggaga tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt
    21181 ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtag ctgcgggtca
    21241 tccgctggcg cagaaagcgg cggaaaataa tcctgaactg gcggccttta ttgacgaatg
    21301 ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac
    21361 tggctttaaa gctgttcacc cattaacggg cgaagaaatt cccgtttggg cagcaaactt
    21421 cgtattgatg gagtacggca cgggcgcagt tatggcggtt ccgggtcacg accagcgcga
    21481 ctacgagttt gcctctaaat acggcctgaa catcaaaccg gttatcctgg cggctgacgg
    21541 ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg
    21601 cgagttcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata aactgactgc
    21661 gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg tttctcgcca
    21721 gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg taatgccaac
    21781 cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg gcattaccag
    21841 cccgattaaa gcagatccgg agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg
    21901 tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc gctacacttg
    21961 cccagagtac aaagaaggta tgctggattc cgaagcggct aactactggc tgccggtgga
    22021 tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc gcttcttcca
    22081 caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg
    22141 tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg aacgtaactg
    22201 ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa
    22261 agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca agtcgaagaa
    22321 caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg ttcgtctgtt
    22381 tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg gtgtggaagg
    22441 ggctaaccgc ttcctgaaac gtgtctggaa actggtgtac gagcacacag caaaaggtga
    22501 tgttgcggca ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt
    22561 gcataaaact atcgctaaag tgaccgatga tatcggccgt cgtcagacct tcaacaccgc
    22621 aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccgaccg atggcgagca
    22681 ggatcgcgct ctgatgcagg aagcactgca ggccgttgtc cgtatgctca acccgttcac
    22741 cccgcacatc tgcttcacgc tgtggcagga actcaaaggc gaaggcgata tcgacaacgc
    22801 gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca
    22861 ggttaacggt aaagtccgtg ccaaaatcac cgtcccggtg gacgcaacgg aagaacaggt
    22921 tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg ttactgtacg
    22981 taaagtgatt tacgtaccag gtaaactcct caatctggtc gttggctaag cgcgggagga
    23041 agcgtgcgat atctggcaac actgttgtta tctctggcgg tgttaatcac cgccgggtgt
    23101 ggctggcatc tgcgtgacac cacgcaggtt ccttccacta tgaaggtcat gatcctggac
    23161 tcaggcgatc cgaacgggcc attaagccgt gcggtgcgta accagttacg tctgaatggt
    23221 gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccctgcg tttgggtaaa
    23281 gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc agagtatcag
    23341 atgatcatga cggtgaatgc gaccgtgttg atccccggcc gtgatatcta cccgattagc
    23401 gccaaagtct tccgttcatt cttcgataac ccgcaaatgg cgttagcgaa agataacgaa
    23461 caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat tcgtaagctg
    23521 ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac cacaacggat
    23581 actccggcaa cgcctgcacg cgtctccacc atgctgggta actgatgatt cggttgtacc
    23641 cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta
    23701 acgatcctct gttattgcag gaaagccagg acgctgttcg ccaggtagct gcggcacaag
    23761 gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat gcgatctttt
    23821 cgttatgcca ggctatgagt ctgtttgcca gtcgacaaac gctattgctg ttgttaccag
    23881 aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga cttctgcatg
    23941 acgacctgct attgatcgtc cgcggtaata aattaagcaa agcgcaagaa aatgccgcct
    24001 ggtttactgc tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc
    24061 agcttccccg ctgggttgct gtgcgcgcaa aacagctcaa cttagaactg gatgacgcgg
    24121 cgaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct caggcactgg
    24181 agcgtttatc gctgctctgg ccagacggca aactgacatt accgcgcgtt gaacaggcgg
    24241 tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg atgggaaaaa
    24301 gtaagcgcgc gttgcatatt cttcagcaac tgcgtctgga aggcagcgag ccggttattt
    24361 tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc cagtctgccc
    24421 atacgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc cggggcatga
    24481 tgggcgaggc gttaaatcgc ttaagtcagc cgcagttacg tcaggccgtg caactcctga
    24541 cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca gagctggaag
    24601 ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac ggttgatatg
    24661 aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg tcatctaaaa
    24721 cccgtggaaa cgctggcgaa tttgattggt ctgacgcggg tcacaatcat ccctaataat
    24781 gttcctccgc atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa
    24841 ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa gcgcaatgcc
    24901 ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaaggacc ggacgtgccg
    24961 ctggcgttta ttattggtca ggattcattg ctgacctttc cgacctggta cgaatacgaa
    25021 acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg
    25081 gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc ggaagatctt
    25141 caccttcagc ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa catctcggcg
    25201 accatcatcc gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg
    25261 gtattgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt acggagggca
    25321 tgatgcgact gtggttaatt cgtcatggcg aaacacaagc gaatgtcgat ggtctgtaca
    25381 gcggtcatgc gcccacaccc ctgaccgcac gcggtattga gcaagcgcaa aatctgcata
    25441 cactgctaga tgatgtttcc tttgacttgg ttttatgcag tgaactggaa cgggcacagc
    25501 ataccgcgcg actggttctc agtgaccgcc agctccccgt tcatatcatc cctgaactca
    25561 acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg caagaagatg
    25621 ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg aacggtgaag
    25681 gatttcaggc attttcgcaa cgtgtggaac gctttatcgc aaggcttagt gaatatcagc
    25741 actatcagaa tattttaatc gtcagccatc agggcgtact gagtctgtta attgctcgtt
    25801 taattggcat gcctgccgag tctatgtggc attttcgcgt tgaccagggg tgttggagcg
    25861 ccattgatat caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg
    25921 tcgagaacgc atgacttttc tgttttttta cgggtaagcc gcaacgacca ttgacagccc
    25981 cgggcaggct gatattctcc gcagccagac tttttccgcc agacacgact ttgtagaaat
    26041 tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc ccactttcga
    26101 aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac tgacagacca
    26161 tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact ccaggatttt
    26221 gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga cgttcaaggc
    26281 aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg tcatgttatg
    26341 tccattgctg accacgttgt gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta
    26401 gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc
    26461 atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag
    26521 ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg ttttaccgag
    26581 tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc ggccggcaaa
    26641 cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca gatgttggcg
    26701 gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg ggatacgccg
    26761 cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtaag tctactgatt
    26821 ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg ctgagcagag ctggtcgctg
    26881 tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg
    26941 gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag ctttgagtag
    27001 aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga gtccgcgctg
    27061 tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg cgtgcttatc
    27121 gccaacctgt ataatctgca aattgttcgc tttaccgact accagacccg ctctaatgaa
    27181 aaccgcatta agctggtgcc tatcgcgccc agccgcggca ttatctacga ccgtaacggt
    27241 atccctctgg ccctcaaccg cactatctac cagatagaaa tgatgccgga gaaagtcgat
    27301 aacgtgcagc aaacgctgga cgctttgcgc agcgtggtag atctgaccga tgacgatatt
    27361 gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctctattcc ggtgaaaact
    27421 aacctaaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt tccgggtgtc
    27481 gaagttaaag gctataaacg tcgttactat ccttacggtt cggcgttgac ccacgtcatc
    27541 ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga cggcaaactg
    27601 gccaactatg cggcaacgca tgatattggt aagctgggca ttgagcgtta ctatgaagac
    27661 gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc
    27721 cgccagttaa aagaagtacc accgcaagcc ggacacgata tttacctgac gctggatctc
    27781 aaactccagc aatatattga aacgctgctg gcgggtagcc gcgcagctgt ggttgttacc
    27841 gaaccgcgta caggtggggt gctggcgctg gtttccacgc ctagttatga cccaaacctg
    27901 tttgttgacg gtatctccag caaagattat tccgccttgc tgaacgaccc gaatacaccg
    27961 ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa accctatgtg
    28021 gcggtttcgg cattaagcgc cggggtgatc acgcgcaata cgacgctgtt tgacccaggc
    28081 tggtggcaac taccagggtc ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg
    28141 cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta tcaggtggcc
    28201 tacgatatgg ggatcgatcg cctctccgaa tggatgggta aattcggtta tggtcattac
    28261 accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga atggaaacag
    28321 aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat cggccagggt
    28381 tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat taatgacggt
    28441 atcgtgaagg ttccgcattt gctgatgagc accgccgaag acggcaaaca ggtgccatgg
    28501 gtacaaccgc atgaaccgcc cgtcggcgat attcattccg gttactggga gctggcgaaa
    28561 gacggtatgt acggtgttgc taaccgccct aatggtacgg cgaataaata ctttgccagc
    28621 gcaccgtaca aaattgcggc gaaatctggt accgctcagg tcttcggtct gaaagcgaac
    28681 gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact gatgaccgcc
    28741 tttgcgccat acaacaatcc gcaagtggct gtcgccatga ttctggagaa cggcggcgcg
    28801 ggtccggcgg ttggtacgct gatgcgccag atcctcgacc acattatgct gggtgataac
    28861 aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca ttaatcatga
    28921 cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc acaatgctgc
    28981 tgattttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc agcggtcagg
    29041 atattggcat gatggagcgt aaaatcggcc aaatcgcgat gggtctggtc atcatggtgg
    29101 tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc tatatcatct
    29161 gtattatttt gctggtggcg gtagatgctt tcggagccat ctctaaaggc gctcaacgct
    29221 ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata gccgtaccac
    29281 tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag aatactggca
    29341 tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct gacctgggaa
    29401 catcaatcct cgttgcgctt tccggtctgt ttgtactgtt cctctctggc cttagctggc
    29461 gtctgattgg cgtcgcagta gtgctggtag cggcgttcat tccgattctg tggttcttcc
    29521 tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct ggacccggaa tcagacccac
    29581 tcggcgcggg ctatcacatt attcagtcta aaattgctat tggctccggc ggattacgcg
    29641 gcaaaggctg gctgcacggc actcagtcac agcttgaatt tctccccgaa cgccatactg
    29701 actttatctt cgcggtactg gcggaagagc tgggattagt gggcattctg attctgctcg
    29761 ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg caaaccacct
    29821 ttggtcgcgt catggctggt ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata
    29881 ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg gtcagttatg
    29941 gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca atccacaccc
    30001 acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg
    30061 atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca gcaacagacg
    30121 gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag cggggcggac
    30181 ccgcgtttcg aaccactgaa cgcgacggca aatcaggatt accagcgcga cggtaaaagc
    30241 tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc aatctatgat
    30301 gccgaaccag gcagtaacct gacggcctct ggcgaagctt tcgatccgac aaagctgacg
    30361 gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct ggctaacggg
    30421 cgaatgatcg tggtgcgcat taatgatcgc ggcccttacg gcaacgaccg cgttatttcg
    30481 ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat
    30541 ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg taccacagtc
    30601 gccaaacaga cttacgccct gcctgcacct cccgatttaa gcggtggcgc gggaacaagt
    30661 tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct aaaaagcgaa
    30721 gatccgaccg gcgcgccggt aaccagtagc ggtttcctcg gcgctccaac gaccttagcg
    30781 cctggtgtac tggaaggcag cgaaccgacg cctgcgccac agcccgttgt tacagcttcg
    30841 tcgacaacgc ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc
    30901 agcggcaact ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc gcaacagtac
    30961 caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc
    31021 tggcggatcc agcttggccc attcgccagc aaagccgaag ccagtacctt gcagcaacgt
    31081 ttgcaaaccg aagcccaatt acagtcattt attactaccg cgcagtagcg taaagcaggc
    31141 atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag tcagatgcct
    31201 gccggtagtg catttgctat agtagggcac ttttttaatt ccatcacgga tgtcgtagtt
    31261 cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct caccacggct
    31321 ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa aactatgatc
    31381 ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa ctccggcaaa
    31441 gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ccagcctgac caaaatgatg
    31501 accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc
    31561 actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg
    31621 ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat taacctgcaa
    31681 tcgggtaacg atgcttgtgt cgccatggcc gattttgccg ctggtagcca ggacgctttt
    31741 gttggcttga tgaacagcta cgttaacgca ctgggcctga aaaataccca cttccagacg
    31801 gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc gctgatcggc
    31861 caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa agaatttacg
    31921 tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag cctgaatgtc
    31981 gacggcatca aaaccggaca cactgacaaa gcaggttaca atcttgttgc ttctgcgact
    32041 gaaggccaga tgcgcttgat ttctgcggtg atgggtggac gtacttttaa aggccgtgaa
    32101 gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt taacccactg
    32161 aaagtaggta aagagttcgc ctctgaaccg gtttggttta gtgattctga tcgcgcttcg
    32221 ttaggggttg ataaagacgt gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa
    32281 gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc
    32341 ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg
    32401 caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa attaatgttc
    32461 catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc ccatatacta
    32521 agcatcagta aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg
    32581 gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca
    32641 aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc
    32701 atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg
    32761 tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactgg
    32821 gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc ggctcctggt
    32881 cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac
    32941 ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac
    33001 gagccaatct cccgggctat gcatgaattc accgataccc gcgataatag tacccttgat
    33061 gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag
    33121 cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact
    33181 tatcacgggc cgggacaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc
    33241 ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg
    33301 ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt
    33361 tgctcactgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc attaaacgtc
    33421 aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg
    33481 gctaaaatat cacaatggaa acccgaagcg acgactaata atattgctcc acgtttactg
    33541 gaaaatattt tagcgctact aaacaatccg gacttcgaat atattaccgc ttaattccat
    33601 acatcaatgg cccaatttac actgggtcat tactcataat acctgctaat gctttacatc
    33661 tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcggtgccac gccttgctcc
    33721 gtaactcacc ccattgatta acctattgaa atggttgatt atggtgagca aaagcgttcc
    33781 gttccaatta gacataagat tattgtaatt ttttaaatat tatttttcca tgaataaaat
    33841 tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag aaagtcgtca
    33901 gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct tctgactatt
    33961 tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc
    34021 ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt
    34081 attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc
    34141 agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa
    34201 cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc tgtcatctat
    34261 cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg
    34321 gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat
    34381 cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcaaggga
    34441 aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactt
    34501 ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga gatgtttccg
    34561 gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact ggttgccaac
    34621 agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg ttgctggccg
    34681 ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac
    34741 aacaaattca tcctgcgtga tcccatcctg cacggagtaa tagatgttat ccgtaatgca
    34801 ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca
    34861 acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata
    34921 ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct
    34981 ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg
    35041 cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac
    35101 agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc
    35161 tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga
    35221 ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat
    35281 cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt
    35341 agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg
    35401 ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccaacactt
    35461 tgcggattgt attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt
    35521 gccggatttc cgcggtcgaa tggatcgtgc tctggatatt ctgactgcaa cgcccccaga
    35581 tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cggcaggtac ggcctggcgc
    35641 agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc
    35701 gaccaagtct ggtctgatgg tgggtctggg tgaaaccaat gaagaaatta ttgaggtaat
    35761 gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag
    35821 ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc
    35881 cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta
    35941 ccacgccgat ttgcaggcga aaggaatgga agttaagtaa tcctgtaata attacttaca
    36001 tctgtctgat aaaaaacccg ctttttgaag cgggtttttt gtatcgaaca agatattgag
    36061 ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg aagatcaacg
    36121 tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa ccctttaatg
    36181 gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag cagaacgacc
    36241 agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac cttcttgaca
    36301 tataaccggc aacagaacgc gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc
    36361 gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa catcataata
    36421 attgcggcgg cgcaaagcga cggttgttta agatgggcag ttgcgcgcgc acctgacgca
    36481 cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct gccgcaatgg
    36541 tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg cactcccccg
    36601 ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt gaccagtgat
    36661 gctctttgag cggcccacga acccaggcgg caggaagtac caggatttca gctccctgta
    36721 aagcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc agaccgacct
    36781 tcatcccttc cacctccagt aacggagcta tttcattacc agcatcaaca tggcgtgatt
    36841 cctgaatggc aaatgcatca tagagatgca gtttggcata acgggcgacg atgtttcctg
    36901 cctgaagtgc caccagcata ttccatgcgc gccccggcgt tgaaggaaga tgaatcgtca
    36961 gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acgtccgagg aattcgcctt
    37021 ccagcagctg tgctgattta accgatagat ctgcatcatg atcatcgcgc gccagcaatg
    37081 cttccggcag gacaaacagc gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac
    37141 aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgca gcaaccagca
    37201 ttgccacctc ctgtcagagt cgtaaaattc gccaacgacg attattcggc gttacactta
    37261 tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt agcagttttt
    37321 attggcggtg gtacgggaag cgtagcgaga tggctgttaa gtatgcgatt taacccgctg
    37381 catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt catcatagga
    37441 atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa agtattaatc
    37501 accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga agtggtgttt
    37561 ttgttacaag agggccgctt tggctgggca ttactgaacg ttttcgtcaa ccttctgggg
    37621 tcttttgcca tgaccgcact ggcattctgg ctgttttcgg cctcaaccgt acactaaaga
    37681 ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc ttacagagcg
    37741 attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc tacgcgctga
    37801 ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac gaacacgtct
    37861 ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa ccacttaacg
    37921 ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac aaatgctgtg
    37981 tcggttatca gtacaccaat tgtggtacgc tttgtccagt gcaactttac taaaaagtga
    38041 taaaagtcgc gttatttttg atgttgctta ttttagttat tgattttaaa gaagttacta
    38101 aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt gccattgttg
    38161 taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac ggaggccaat
    38221 ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac gccagcggtg
    38281 aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact ttcccatccg
    38341 tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc atacaggcca
    38401 tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca cggtcgctcg
    38461 ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt gatggcagga
    38521 atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc tctaaacgtt
    38581 gtcatcctct catctgcgtt agcaaaaact ttaccaacgc tgattaactg aataaaaaca
    38641 aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac atttatcaaa
    38701 aatctactac tagcatagca aagctacaat taacataacc ttaatagacc caacataaag
    38761 aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat ttccgcgcat
    38821 cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caaaaaagaa aaaccaagtc
    38881 ttttttgatg acaaaatgca atcaaggaaa aattatttta tttttttaac gagatacaac
    38941 aatcatctta acgaagtata taatatatat cgtcaaccca tgctcatatt agattatttt
    39001 ttgagcgcac agccgcagct aacaccattg caattaacaa atttgcatca atccaccatc
    39061 aatttacaca cattattatg tgataattgc caaccgctaa atatgcgttt tgttatctat
    39121 gtataaaaac agcaacttca atgtcttaat ggcagttttt cttgatttta atcagcattc
    39181 atcgccaatt tattgggcat attttttcct taagctttag gaagttttta ttatttactt
    39241 tgggcctgga gacaggaaaa attatgctga cattcattga actccttatt ggggttgtgg
    39301 ttattgtggg tgtggctcgc tacatcatta aagggtattc cgccactggt gtgttatttg
    39361 tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg ttaccgtcca
    39421 gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa atattactaa
    39481 tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggcttt gccgcttaca
    39541 tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca ttgcagtata
    39601 ttaactcccc ttacctgctg atgattgccg cctattttgt tgcttgtctg atgtcactgg
    39661 ccgtctcttc cgcaaccggt ctgggggttt tgctgatggc aaccctgttt ccggtgatgg
    39721 taaacgttgg tatcagtcgt ggcgcagcag ctgccatttg tgcctccccg gcggcgatta
    39781 ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga
    39841 ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc ggcatggcga
    39901 tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct catgaaatgt
    39961 tagatgtcag tgaaatcacc accactgccc ccgcgtttta tgccattttg ccgttcacgc
    40021 cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac atcatcacta
    40081 ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc tttaataccc
    40141 agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg tttgctaacg
    40201 tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc atcggcttta
    40261 ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc ctgatgctgg
    40321 tattggtgat tctgaccatg ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt
    40381 atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac ccggcgtatt
    40441 tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg
    40501 tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct
    40561 cggtgccggt gcttgttggg ctagtgattg ttatcgttgc tacagagctg atggtgccag
    40621 gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc
    40681 tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat
    40741 ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg cagtggtgcg
    40801 gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa
    40861 cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg
    40921 ttcaccgcga gcataggcat taaacatctc atcaatttgc ttctggctgg cgctatcaat
    40981 actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca gtgtattgat
    41041 tcacttgaag tacgaaaaaa ccgggagaac attggattat tcgggatctg atgggattag
    41101 atttggtggg gcttgcaagc ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa
    41161 agatagtaaa aaattgcttt tgtttcattg aaaaaacgaa aaacaaaaac actataaatc
    41221 atttcaatca tagcttcaaa aaacgttcaa aaccgataac aaccaggctg tcatcaaatg
    41281 actcatatca caaatcagct catgccgttt aggtatgtta catgtgtgat tatgtgaggt
    41341 gaagtatgtt ttagctggct catggttgtt atacggcttt tttcgcatct tgtggttcca
    41401 acaaaggagc tacaggctct tcctgtttat gaacaccata cattgacaaa atctcttggc
    41461 gagtttctgg aggtaattta tgcagcgcca aagctgtttg tagtgtttgg cgatcaatac
    41521 ccagcgttgc caatagggtg tcaacggaga tggaggggcg ttcttgcatt aatccggaaa
    41581 gcccatattt agccatgttt agctctatct ctcggacttc attttctcct agtttattga
    41641 ttaacaggga gaacattttc aatgtatcag cattcatttc ttcgatagca attttttgca
    41701 tagccggtat tgccgggata tattcaaccc caggcccgcg acgcccttct tgtgaacgct
    41761 tcggccatcc ttctttcttt gctcgtttat gcaccccctg tattgtccca ggcattccat
    41821 ccagttcagt aatttgtttg ggtgtcatcc actggtttat ctcatttgcg cttttcataa
    41881 actaagttta ccagatttat ctatataaaa atcaatggat tacaaaataa accacattaa
    41941 ctgatataaa ccggtttggt ttatttggta atgtataaaa cagataatca agacgaatta
    42001 tttgttttgg taacccttta tcatacgcaa tatggttggt gtgcgaaaga tgagtaacga
    42061 ttggcatcgg gccgacgtga ttgcggccct caaaaaaaca ggaacccctc tggcaaaatt
    42121 gtccagacgt tcaggttatt caaccgggac gcttgcaaat gcattagtca ggccatggcc
    42181 taaaggtgaa cgcattattg ctgacgcctt aaacatgaaa cctgaggaaa tttggcctag
    42241 tagatatcta agggatcaca atgagagagt ggatatcagc aattgaatgc atgggtttat
    42301 ctggtttacc tggttcatta atgggggttc acaaaagagc tcagagagaa ggatggctca
    42361 agagaagaag agaaggaatt agaggggttg cttacgaata ccatatatct tctcttccta
    42421 acattgctcg agctgaagtt ttgctccaac aaggaaaatt taaaaccagt cagggatgtt
    42481 ttgaaatcgc ccgccccacg ctggaagctc atgattatga tcgtgaggca ctatggagca
    42541 aatgggataa agccagcgat tcccagcgca gcctggctga aaaatggttg ccctcaattc
    42601 aggccacaga cgaaatgctg aaccagggga tttcaacgaa aacggctttt gcgaccgttg
    42661 cagggcatta ccaggtcagc gcatccactt tgcgggacaa gtattaccag gtacagaagt
    42721 ttgcgaagcc tgactgggcg gctgcacttg ttgatggacg tggagcatcc cgtcgcaatg
    42781 ttcacaaaag tgaatttgac gaggatgcct ggcagtttct gattgcagat tatctgcgac
    42841 cggaaaaacc cgccttccgc aaatgttatg agcgtctgga actggcagcc cgcgagcatg
    42901 gctggagtat tccctcccgt gccacggcct ttcgccggat tcagcaactg gacgaggcaa
    42961 tggttgttgc ctgtcgtgaa ggtgaacatg cactgatgca tctgataccg gcacagcagc
    43021 gaactgtgga acacctggac gccatgcagt ggatcaacgg cgacggttat ctgcataacg
    43081 tctttgtacg ctggtttaac ggtgatgtga tccgtccgaa aacatggttc tggcaggatg
    43141 tgaaaacccg aaaaattctg ggctggcgct gcgatgtgag cgagaacatt gattcaattc
    43201 gcctctcgtt catggatgtt gtgactcgct acagtatccc ggaggatttt cacatcacca
    43261 ttgataacac ccgtggtgct gcgaataaat ggctgacggg aggcgcgccc aatcgctacc
    43321 gctttaaggt aaaagaggac gatccaaaag gactgttttt actgatgggt gcgaaaatgc
    43381 actggacaag cgttgttgcc ggtaaaggct ggggccaggc aaaacctgtt gaacgtgctt
    43441 tcggtgttgg tgggcttgag gaatacgttg ataagcatc
//
LOCUS       DAJTYH010000022        42461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTYH010000022 DAJTYH010000000
VERSION     DAJTYH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..306)
                     /gene="ssb1"
                     /locus_tag="OO609_001210"
     CDS             complement(<1..306)
                     /gene="ssb1"
                     /locus_tag="OO609_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6632704.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     "
     gene            560..3382
                     /gene="uvrA"
                     /locus_tag="OO609_001211"
     CDS             560..3382
                     /gene="uvrA"
                     /locus_tag="OO609_001211"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6632705.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3417..3773)
                     /locus_tag="OO609_001212"
     CDS             complement(3417..3773)
                     /locus_tag="OO609_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6632706.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(3777..4193)
                     /locus_tag="OO609_001213"
     CDS             complement(3777..4193)
                     /locus_tag="OO609_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6632707.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4304..5017)
                     /gene="aphA"
                     /locus_tag="OO609_001214"
     CDS             complement(4304..5017)
                     /gene="aphA"
                     /locus_tag="OO609_001214"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6632708.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5419..5623
                     /locus_tag="OO609_001215"
                     /pseudo
     CDS             5419..5623
                     /locus_tag="OO609_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6126..7319)
                     /gene="tyrB"
                     /locus_tag="OO609_001216"
     CDS             complement(6126..7319)
                     /gene="tyrB"
                     /locus_tag="OO609_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6632709.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7572..8651)
                     /gene="alr"
                     /locus_tag="OO609_001217"
     CDS             complement(7572..8651)
                     /gene="alr"
                     /locus_tag="OO609_001217"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6632710.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8704..10119)
                     /gene="dnaB"
                     /locus_tag="OO609_001218"
     CDS             complement(8704..10119)
                     /gene="dnaB"
                     /locus_tag="OO609_001218"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6632711.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10202..11185
                     /locus_tag="OO609_001219"
     CDS             10202..11185
                     /locus_tag="OO609_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6632712.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11351..11593)
                     /gene="pspG"
                     /locus_tag="OO609_001220"
     CDS             complement(11351..11593)
                     /gene="pspG"
                     /locus_tag="OO609_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6632713.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11727..12764)
                     /gene="dusA"
                     /locus_tag="OO609_001221"
     CDS             complement(11727..12764)
                     /gene="dusA"
                     /locus_tag="OO609_001221"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6632714.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13127..14112)
                     /locus_tag="OO609_001222"
                     /pseudo
     CDS             complement(13127..14112)
                     /locus_tag="OO609_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14430..14945
                     /gene="zur"
                     /locus_tag="OO609_001223"
     CDS             14430..14945
                     /gene="zur"
                     /locus_tag="OO609_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6632715.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(14987..15196)
                     /locus_tag="OO609_001224"
     CDS             complement(14987..15196)
                     /locus_tag="OO609_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6632716.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15312..16637)
                     /gene="dinF"
                     /locus_tag="OO609_001225"
     CDS             complement(15312..16637)
                     /gene="dinF"
                     /locus_tag="OO609_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6632717.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16710..17318)
                     /gene="lexA"
                     /locus_tag="OO609_001226"
     CDS             complement(16710..17318)
                     /gene="lexA"
                     /locus_tag="OO609_001226"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6632718.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17428..17796)
                     /gene="dgkA"
                     /locus_tag="OO609_001227"
     CDS             complement(17428..17796)
                     /gene="dgkA"
                     /locus_tag="OO609_001227"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6632719.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17967..20390
                     /gene="plsB"
                     /locus_tag="OO609_001228"
     CDS             17967..20390
                     /gene="plsB"
                     /locus_tag="OO609_001228"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6632720.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20545..21417)
                     /gene="ubiA"
                     /locus_tag="OO609_001229"
     CDS             complement(20545..21417)
                     /gene="ubiA"
                     /locus_tag="OO609_001229"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6632721.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21430..21927)
                     /gene="ubiC"
                     /locus_tag="OO609_001230"
     CDS             complement(21430..21927)
                     /gene="ubiC"
                     /locus_tag="OO609_001230"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6632722.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22150..23730)
                     /locus_tag="OO609_001231"
                     /pseudo
     CDS             complement(22150..23730)
                     /locus_tag="OO609_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(23959..24879)
                     /gene="malM"
                     /locus_tag="OO609_001232"
     CDS             complement(23959..24879)
                     /gene="malM"
                     /locus_tag="OO609_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6632723.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25031..26371)
                     /gene="lamB"
                     /locus_tag="OO609_001233"
     CDS             complement(25031..26371)
                     /gene="lamB"
                     /locus_tag="OO609_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6632724.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26443..27558)
                     /gene="malK"
                     /locus_tag="OO609_001234"
     CDS             complement(26443..27558)
                     /gene="malK"
                     /locus_tag="OO609_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6632725.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            27923..29113
                     /gene="malE"
                     /locus_tag="OO609_001235"
     CDS             27923..29113
                     /gene="malE"
                     /locus_tag="OO609_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6632726.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29267..30811
                     /gene="malF"
                     /locus_tag="OO609_001236"
     CDS             29267..30811
                     /gene="malF"
                     /locus_tag="OO609_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6632727.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            30826..31716
                     /gene="malG"
                     /locus_tag="OO609_001237"
     CDS             30826..31716
                     /gene="malG"
                     /locus_tag="OO609_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6632728.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32087..33562
                     /gene="xylE"
                     /locus_tag="OO609_001238"
     CDS             32087..33562
                     /gene="xylE"
                     /locus_tag="OO609_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6632729.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33606..34016)
                     /gene="psiE"
                     /locus_tag="OO609_001239"
     CDS             complement(33606..34016)
                     /gene="psiE"
                     /locus_tag="OO609_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6632730.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34142..34285)
                     /locus_tag="OO609_001240"
     CDS             complement(34142..34285)
                     /locus_tag="OO609_001240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632731.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34286..34564
                     /locus_tag="OO609_001241"
     CDS             34286..34564
                     /locus_tag="OO609_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632732.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34611..36707)
                     /locus_tag="OO609_001242"
     CDS             complement(34611..36707)
                     /locus_tag="OO609_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6632733.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAISWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(36707..37444)
                     /locus_tag="OO609_001243"
     CDS             complement(36707..37444)
                     /locus_tag="OO609_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6632734.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37441..38079)
                     /locus_tag="OO609_001244"
     CDS             complement(37441..38079)
                     /locus_tag="OO609_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6632735.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38193..38435)
                     /locus_tag="OO609_001245"
     CDS             complement(38193..38435)
                     /locus_tag="OO609_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632736.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(38898..40547)
                     /gene="pgi"
                     /locus_tag="OO609_001246"
     CDS             complement(38898..40547)
                     /gene="pgi"
                     /locus_tag="OO609_001246"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6632737.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41072..42421
                     /gene="lysC"
                     /locus_tag="OO609_001247"
     CDS             41072..42421
                     /gene="lysC"
                     /locus_tag="OO609_001247"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6632738.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10572 a        10905 c        10426 g        10558 t
ORIGIN      
        1 gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc catttacggg tacgcagctg
       61 accttcgata taaacctgag aacctttacg cagatattcg ctcgccactt ctgccagttt
      121 gccgaacagc acaacgcggt gccattcagt ctgttctttc atctcgccgg tcgctttatc
      181 acgccaggat tcggaagtag ccagcgtaat gttggcaact gcgccaccat ttggcatgta
      241 gcgtacttcc gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct
      301 ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa gtgtaaacgc gcgattgtac
      361 cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa acctggccag
      421 acattgttac acaacactcc ggataatgca ttccaatact gtatattcat tcaggtcaat
      481 ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg caaaaaagcg
      541 tttaatccgg gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca cccataatct
      601 caaaaacatc aacctcgtta tcccccgcga caagctcatt gtcgtgaccg ggctttcggg
      661 ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc gaagggcagc gccgttacgt
      721 tgaatccctt tccgcctacg cgcggcagtt tctgtcactg atggaaaagc cggacgtcga
      781 tcatattgag gggctttctc ctgccatctc aattgagcag aaatcgacgt ctcataaccc
      841 gcgttctacg gtggggacaa tcaccgaaat ccacgactat ttgcgtttgt tgttcgcccg
      901 cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg gcggcgcaaa ccgtcagcca
      961 gatggtggat aacgtgctgt cgcagccgga aggcaagcgt ctgatgctgc tcgcgccaat
     1021 cattaaagag cgcaaaggcg aacacaccaa aacgctggag aacctggcaa gccagggtta
     1081 catccgtgct cgtattgatg gcgaagtctg cgatctttcc gatccgccga aactggaact
     1141 gcaaaagaaa cataccattg aagtggtggt tgatcgcttc aaggtgcgtg acgatcttac
     1201 ccaacgtctt gcggagtcgt ttgaaaccgc gctggaactt tccggtggta cagcggtagt
     1261 ggcggatatg gacgacccga aagcggaaga gctgctgttc tccgccaact tcgcctgccc
     1321 aatttgcggc tacagtatgc gtgaactgga gccgcgactg ttttcgttta acaacccggc
     1381 aggtgcctgc ccgacctgtg acggccttgg cgtacagcaa tatttcgatc ctgaccgcgt
     1441 gatccaaaac cccgagctgt cactggctgg cggtgcgatc cgtggctggg atcgccgcaa
     1501 cttctattac ttccagatgc tgaaatcgct ggcagatcac tataagttcg acgtcgaagc
     1561 gccgtggggc agcctgagcg cgaacgtgca taaagtggtg ttgtacggtt ctggcaaaga
     1621 aaacattgaa ttcaaataca tgaacgatcg tggcgatacc tccatccgtc gtcatccgtt
     1681 cgaaggcgtg ctgcacaata tggagcgccg ttataaagag acagaatcca gtgcggtacg
     1741 tgaagaatta gccaagttta tcagcaatcg cccatgcgcc agctgcgaag ggacgcgtct
     1801 gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg ctgcctgcta tctccgacat
     1861 gagcatcggt catgcgatgg aattcttcaa caatctcaaa ctcgctggtc agcgggcgaa
     1921 gattgcggaa aaaattctta aagagatcgg cgatcgcctg aaattcctcg taaacgtcgg
     1981 cctgaattac ctgacacttt cccgctcggc agaaacactt tccggcggtg aagcccagcg
     2041 tatccgtctg gcgagccaga ttggtgcggg cctggttggc gttatgtacg tgctggacga
     2101 gccgtctatc ggcctgcacc agcgcgataa cgagcgcctg ttgggtacgc ttatccatct
     2161 gcgcgatctc ggtaataccg tgattgtggt ggagcatgac gaagacgcaa ttcgcgccgc
     2221 tgaccatgtg atcgatatcg gtcctggtgc gggtgttcac ggcggtgaag tggtcgcaga
     2281 aggtccgctg gaagcgatta tggcggtgcc tgaatcgttg accgggcagt acatgagcgg
     2341 taaacgcaag attgaagtgc cgaagaaacg cgttccggcg aatccagaaa aagtgctgaa
     2401 gctgacaggc gcacgcggca acaacctgaa ggacgtgacg ctcacgctgc cagtcggtct
     2461 gtttacctgc atcacagggg tttcaggttc cggtaaatcg acgctgatta acgacacact
     2521 gttcccgatt gcccaacgcc agttgaatgg tgcgaccatc gccgaaccgg caccgtatcg
     2581 cgatattcag ggactggagc atttcgataa agtgatcgat atcgaccaaa gcccaattgg
     2641 tcgtactcca cgttctaacc cggcgaccta taccggcgtg tttacgcctg tgcgcgaact
     2701 gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg ccaggacgtt tcagctttaa
     2761 cgtccgtggc gggcgctgcg aagcctgtca gggcgacggt gtgatcaaag tggagatgca
     2821 cttcctgccg gatatctacg tgccgtgcga tcagtgcaaa ggtaaacgct ataaccgtga
     2881 aacgctggaa attaagtaca aaggcaaaac catccacgaa gtgctggata tgaccatcga
     2941 agaggcgcgt gagttctttg atgcggtgcc agctctggcg cgtaagctgc aaacgttgat
     3001 ggacgttggt ctgacgtaca ttcgcctggg gcagtccgca accacacttt ctggtggtga
     3061 agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc agacgctgta
     3121 tattctcgac gagccgacca ccggtctgca cttcgccgat attcagcaac tcctcgacgt
     3181 gctgcataaa ctgcgcgatc agggcaatac cattgtggtg attgagcaca atctcgacgt
     3241 gatcaaaacc gctgactgga ttgtcgacct gggaccagaa ggcggcagtg gcggcggcga
     3301 aatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc gaagcttcgc atacggcacg
     3361 cttcctcaag ccgatgctgt aatcgttaag gccgctttct gagcggcctt ttcctttcag
     3421 agttgtacca gcaatttacg cttttcttcc ggtagtaaat tcactgcctg ctgataagac
     3481 gcatccacca gataatagat ttgcgaatcc ggcagcgaac cgtcgagata cacggtgctc
     3541 cagtgcgctt tattcagatg gcggctagga cgcacatcgc tgtgctgctg acgtagcagc
     3601 tccgccagct ccgggctggt tttcagcgaa acagctgggc gattttctac ttctttcacc
     3661 atcgcaaaca gtacatcttc cactttgatc tgcgtcgctt tccagtcgtt atgcacgctc
     3721 tgttctgcgc ttggttttgc catgcaatat tgtagcaact ccgaaatggt cattttttac
     3781 tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg ttcccccagc
     3841 cagatgcctt gccaagtgcc ggtctgaata cgccctttat gtaccggcaa tacaagcgat
     3901 gttcccagca ttgaggattt gatatgagaa ggcatatcgt ctgctccctc atagtcatgc
     3961 tcataatttc cgttgtcggg aacggtgcgg aggaaaaaac actccatgtc gtggcgtacg
     4021 gtgggatcgc agttctcatt aagtgtcaga gaggcggagg tatgttgcag caacagatgc
     4081 agtaagccga tgttaacgcg cggcatatca gccagctgat tcagaatttc atccgttacc
     4141 agatgaaacc cacgagattt ggcgctaagc gtgagcgtct tttgatacca catgtgctgc
     4201 tccttgataa aactctctta atcagtttgc agcaagacag cgaaaggata aaggtgtgat
     4261 taaaaaaaca gcattcggga gagcatcact ctctcccgct ctgtcagtat tctgaattga
     4321 cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta gagttggagg
     4381 cgcgcagaat gcggatacca cgagcgccga catcgcgtgc ggcggtaata tcgttatcgg
     4441 aatcgccata aaaaattcgg atatttttat cctgcagcca ttgcgatttt gtattttgcc
     4501 ctggtttatc gcccgcaaag attaccggat tcatgttggt ggcaggaata tgaaaattat
     4561 ccgccagcgt ttttgaaacc gtttctgttt tcgtcgggct acgaccagtc acaaagaaga
     4621 tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg agcgacctct tttggaatgc
     4681 tgaattcatc ccagccatta ttcatttttt cccagaacac aggatttttc agataatctt
     4741 cgctttctgg cgagaaggtt tttttgccgc gccagaaccc cggactggaa aaaagtaccg
     4801 tgtcatcgat atcaaacccc accgccattg gcggacgccc tgcgaggcta ttttcaattt
     4861 gtgcgaccga aacccaatga attggtgcct gttcagcaag cctggcaacg ttagtcccag
     4921 ggttaagcgg tgaaggagat gaggccaggg caacagcgga actgtttaga gcgaacaata
     4981 agcaaacggc actgattgcc tgtgtgatct tgcgcatatt tttccctaaa tagtcagtat
     5041 gttgaaactt tttattgtga gatttgttgc aaaaaccatc tgaccataac gccagctgcc
     5101 ggggaaagga aggattatct gcggtttttg tgatgaaaag caggatgatt atatttaacc
     5161 cagtaaatac gttcctgtct ttttttgtat aaaaaatgtt tattactcga tgtaaggata
     5221 cattaatttt acgtttatta tgatgaatcc tcggcaaatg agcgaaattt gatagaacat
     5281 ttatctgctg tacgatttaa tgaacttaat gatacagcat tacaaaaaca atcgaagttt
     5341 ataaagatga tttctgattg accagccccc taccctacaa tggtgattaa ttttattact
     5401 cgcgatggag atgatgatat gaatatatca tatgtgaata gcaacaaaac gacatcttta
     5461 cctgttgagc tagatgcact aaataataaa gatatctctt atgccaaaga tttcttttta
     5521 tacatagaaa cacagttaaa aattgcaaaa gatttttgta gacctggaga agaagtatca
     5581 agttctattg caagtaaagt ttttcacgca tttattgatt tagttaacaa aatcaggggt
     5641 aagaaagact ttatgtatat ttgcacgctt tgctgttttg ccgaggaggt taaaggtgat
     5701 tattctcatt acaggacctt tttatttgat attggtaatc aatataaggt taaacttaca
     5761 caaagcggaa aaaagagttc tctttaacat tagaatttaa cgatactata attgaatctc
     5821 agaaagtcac aggcaataag gcaaagcata ttcttgagga tatagaaata ttctatcgta
     5881 acaaacccga tacttattat taaatttaat aatggttaga tgtgcagaaa ttattactcc
     5941 acacgaaaaa atagggaaag gaattccaga taattgggat aaacttaaca ataaaagaac
     6001 tgacaacgtt ttattatctc tcacgtagaa caacggcatc aaaaaaatca tctacaaaga
     6061 aatagtgaaa gcgacgacag tttaatttca ctgcaggctg ggtaactcca gcctgctttc
     6121 ctgcattaca tcaccgcagc aaacgccttt gccacacgtt gtacatttgc cgtatttaac
     6181 ccggcgacac acatgcgacc gctggcgatg agatagacac caaattcttc acgtagtcgg
     6241 tcaacctgag cggcacttaa accggtataa ctgaacatgc cgcgctgatt aagcagataa
     6301 tcgaaattgc gttctggcat ctctgtgctt aataccttca ccaattcctg acgcattgcc
     6361 agaatgcgag tacgcatctc ttctacttcc gccagccagc tggctttcaa tgcctcgtca
     6421 ttcagcactg cagccaccac ctgcgcacca aaattcggcg ggctggagta gttgcggcga
     6481 actgttgctt tcaattgccc cagtacgcgg ccagcggctt cggcatcttc acacagaaca
     6541 gaaagtccgc cgacgcgctc gccgtaaagg gagaaaattt tcgagaacga attgctcacc
     6601 agagcgggta atccagcact ggcaatggcg cgaatagcgt aggcatcctc ttccataccg
     6661 gcactaaatc cttgataggc aatatcgagg aatgggataa gctcgcgggc tttgagaatt
     6721 tcaatcaccg catcccactg atcattagtg agatcggcac ccgttgggtt gtggcaacat
     6781 ggatgcagca acacaatact gcgggcaggt aatgttttca gcgtcgccaa caggtcatta
     6841 aagcgcacgc tgttagtcac ttcgtcatac caggggtaag tactcacttc gaatccagcc
     6901 ccggcgaata ttgctacgtg gttttcccaa gtaggatcgc tgacccagac gcctgattcc
     6961 gggaagtagc gtttcaggaa atccgcgccc actttcaatg cccctgagcc gccaagggtt
     7021 tgaatggttg ctacgcgctg ttgttgcagt accggatggt ccgcaccaaa cagcagcggc
     7081 gcaatggcat ggcgatagct gttaagccct tccatcggta aataaagcga agcgccatgg
     7141 ggctgcgcat tcaggcgcgc ttccgcctcc gccacggctt tcaattgtgg aataattccg
     7201 tcttcgttgt agtacagacc gatacttaaa ttcactttgt cgctgcgagg gtcttcttta
     7261 aaacgctcca taagcgtaag aatcgggtcg ccagcgtagg cgtcaacttt ttgaaacacg
     7321 cgatggttct ccaggtttac gggcaggtgg ttaaaacaca ataaaccgga agaaggcgaa
     7381 gatcgagtgg atgttcagga gcgaacggca attagcaaca gagtgagact aatgataaac
     7441 gtacatccgc cagaggcacg acctttataa gagcatgaaa gaacatcaac ttattgaatt
     7501 tttaggattt tattggccgg ataaggcatt cacgccgcat ccggcacaga caatcaaata
     7561 ttacagaacg attaatccac gtatttcatc gcgacccttg aagtcaggcg cgtaataagt
     7621 tcgtaagcgc ttactttcgt catttcagcg atacgttcta cgggcaaacc ttcgccccat
     7681 aaaatgaccg gatccccggc tttgtcctgc gcctgtggac ctaagtctac gcagatcata
     7741 tccatcgcca cgcgcccgac aatcggtact tcgcgaccgt tcaccagcac tggcgtaccg
     7801 gacggcgcgg cgcgcggata accatcgcca tagcccatcg cgactacgcc aagacgggta
     7861 tcacgttcgc ttacccaggt tccaccataa ccaacaggct ctccggcttt atgctcacgc
     7921 acggcaatca ggctggaggt tagtgacatc actggctgac agccaaaatc ggcaccggtg
     7981 gagcgatctt ccagcggcga gacgccataa agaatgatgc ccgggcgcac ccagtcaaaa
     8041 tgcgactgtg gccacagcag aatgccaccc gacgcggcaa tggaacgttg accaggtttg
     8101 ccttcgcaaa aggtattaaa gatagcgagt tgtttctcgg ttgcgccaca ttttggttca
     8161 tccgcgcgcg caaaatggct gacgatattc accggctgac gaacgttttt gcactgggtc
     8221 aggcgatgat aaaacgcctc agcctgttcc ggccttacgc ccagacggtg cataccggta
     8281 tcgagtttca tccagacggt aaccggctcg tccaagctag cctcttccag cgcagccagc
     8341 tgttcttcgt tatgcacggc ggtatgaaaa tgttgcgcag aaatcgtcgg cagatctctg
     8401 gcatcaaaaa agccttcgag taacagtaca ggtttggtga ttccccccgc acgcagtcgc
     8461 agagcttctt cgagacgggc tacgccaaag gcgtcagcat cggggagcgt tcgcgcggtc
     8521 tcaagaagac cgtgaccata agcgttcgct ttcaccaccg caaccatttt actggcaggt
     8581 gccagttcac gaagacgttg caggttgtgt cgcagagcgc ggcggttaat cacaacagtt
     8641 gccgcttgca tttgtgttcc ttgataattg tttgctttaa ttacctaatt cataaaataa
     8701 ttattattcg tcgtcgtact gcggccccgc atagttgtcg aagcgcgacc attgaccgtt
     8761 aaaggtcagg cgtaccgtcc cgattgggcc gttacgttgt ttaccgataa taatttccgc
     8821 gatgcctttt aaatcactgt tttcgtgata cacctcatca cgatagataa acatgatcaa
     8881 gtccgcatcc tgctcgatag agccagattc acgcaggtcg gagttgaccg gacgtttgtc
     8941 ggcacgttgt tccagagaac ggttcaactg ggacagcgcc accaccggca cgttcagttc
     9001 tttcgccagt gctttcagcg agcgggagat ttctgcaatt tccagcgtac ggttatcgga
     9061 aagcgccggt acgcgcatca gttgcaggta gtcgatcatg ataagcccga tgccgccgtg
     9121 ttcacgggca atacggcgtg cgcgggaacg cacttccgtt ggcgtcaggc cggaagagtc
     9181 atcgatatag atattgcgtt tttcgagcaa aatacccatg gtgccggaaa tgcgcgccca
     9241 gtcttcatca tcgagctgcc cggtacggat tttagtctgg tcaacgcgcg acagcgacgc
     9301 cagagaacgc atcatgatct gttctgatgg catctccagc gagaagataa gtactggttt
     9361 atcctgcaac attgccgcgt tttcgacgag gttcatcgca aatgttgttt tacccatcga
     9421 cggacgcgcg gcgacgatga tcaaatccga cggctgcaag ccagcggttt ttttgttgag
     9481 atcgtcataa ccggtgttta ccccggtaac gccatcgtgt ggctgctgaa acaactgctc
     9541 aatacgcgcc acggttgcgt cgagcacatc ggcgatgttc ttcggccctt cgtctttgtt
     9601 tgcacgactt tcggcaattt taaagacgcg ggattcagca aggtccagca gatcttcgct
     9661 ggtacgcccc tgcggatcaa aaccggcttc agcaatctca ttcgcaaccg agatcatctc
     9721 acggacaacg gcacgttcac gcacgatgtc cgcataagca ctgatgttcg ccgcacttgg
     9781 cgtatttttt gacagctctg ccagataagc aaaaccaccg acgctatcga gttgcccctg
     9841 gcgttccaac gattccgcaa gggtaatcag atcgatagga ctaccgcttt cctgcaaacg
     9901 cgccatttca gtaaagatat gacggtgtgg gcgggtgtaa aaatcgtctg ctaccacacg
     9961 ctcggctaca tcatcccagc gttcgttatc cagcattaaa ccgcccaaca ccgactgctc
    10021 cgcttcgatc gagtgcggag gcactttcag cccggcaact tgtggatcgc gttcgcgggg
    10081 ttcagcctgc tgtttgttga agggtttatt tcctgccata gtgaatggag ttaccgaaat
    10141 aaagaatggg tcgaaacttt accatatgaa gcagaccctg acgatacgtt ctggaggaca
    10201 catggcaaca cgaattgaat ttcacaagca cggtggcccg gaagtacttc aagccgtaga
    10261 gttcactcct gccgatccgg cggagaatga aatccaggtc gaaaataaag ccatcggcat
    10321 caattttatc gacacgtata tccgcagcgg cctttacccg ccgccatcgc tacccagcgg
    10381 attaggtacc gaagcggcag gcatcgtgag taaagtcggc agtggtgtaa agcatattaa
    10441 ggctggcgat catgtagtct atgcgcagtc ggcgttaggc gcttacagct ctgtgcataa
    10501 cattaatgcg gataaagcgg cgattctgcc tgcggcaatt tcttttgagc aagctgcggc
    10561 atccttcctg aaaggcttaa cggtttatta tctgctgcgc aaaacctatg aaattaaacc
    10621 cgatgaacag ttcctgttcc acgcagcggc tggcggcatt ggcttaattg cctgccagtg
    10681 ggcaaaagcc ctgggcgcga aactcatcgg caccgtagga accgcgcaaa aagcgcagag
    10741 cgcgctaaaa gccggcgcgt ggcaggttat taactatcgt gaagagaatc tggtcgagcg
    10801 attgaaagag atcaccggcg gtaagaaagt gcgcgtagtg tacgattccg tgggcagaga
    10861 cacctgggaa cggtcgctgg attgcctgca acgccgcggc ttaatggtca gttttggcaa
    10921 ctcatcaggt gcggttaccg gtgtgaactt aggcattctc aatcaaaaag gctcgttgta
    10981 tgtgacacgc ccttccctgc aaggctatat caccacgcgg gaggaattaa ccgaggccag
    11041 taatgaactg ttctctttga ttgccagcgg tgtgattaag gtcgatgtcg ccgagcagca
    11101 gaaatatccg ctgaaggatg cgcagcgtgc gcatgagatt ctggaaagcc gggcgacgca
    11161 aggttccagc ctgctgattc cataaaagaa atagggcttc cacctgggaa gccctttctt
    11221 tttatagttc ggctgtatgt agggtacagc acgatgaatc tgttagaggc gcaatagtga
    11281 cagatttgat tatcaattcc tattttgttc taaggataaa accttaggtt gtgatcatcc
    11341 gcacaatccc ttagtaacgc cagcggtcat aacgctgata tttcggcatt tttggtgctt
    11401 taatcgcctt aataacccac accaccgcaa tcgccagtag taaccacggc agcaacttaa
    11461 tcatcaatgc cagcataccg ccgaggaaca taatggccgt cgccacaacc agcgcggcga
    11521 taatgcccag caacgaaacg ccggtgacca tcagcatgac aaaaaagcca atcacaaaaa
    11581 gtagttccag catgatgctc tcccaaatat gaaatctctt gctggcatta caagaatcat
    11641 gccaaaaata atctattgat ttaacagcaa aacgccccgc gacggtgcgc agggcgtggt
    11701 gaatttgact actttttggt gaaaagttaa cgcttatccg ccaccagttt gagcgcgtgt
    11761 tccagcacgt taatgtcagc acccgcttta tgggcatttt cacttaaata acgccgccac
    11821 tgccgcgcgc caggaatacc ctggaacaaa cccagcatat gccggataat atggccgaga
    11881 tacgtcccct ggctgagttc atgctcaatg tacggataca tggcacgcac tactgccacc
    11941 ggatcggcat cggtatccga ggaaccaaag atctcccggt ctaccgccgc cagaataccc
    12001 ggattctgat acgcctcgcg cccgaccatc acgccatcca tatgttgcaa atgcgcttta
    12061 gcttcttcca gcgacttgat accaccgtta atcgacattg tcagatgcgg gaagtcacgc
    12121 ttcagttgat acacacgcgg ataatcgagc ggcgggattt cacggttttc tttcgggctt
    12181 aaccccgaaa gccaggcttt acgtgcgtgg atgatgaaca tctcacactc gcctttgccg
    12241 gaaacggtat tgatgaaatc gcagagaaat tcatagctgt cctggtcgtc gatgccaata
    12301 cgcgttttca ccgtcaccgg aatcgacacc acatcgcgca tcgctttcac gcagtcggca
    12361 accagctgcg cattacccat cagacacgca ccaaacatgc cgttctgcac ccggtcagac
    12421 gggcagccga cattcaggtt gatctcatca tatccgcgcg cttctgccag ctttgcacac
    12481 tgcgccagcg ccgccggatc gctaccgccc agttgcaacg ctaccggatg ttcttcttca
    12541 ctgtacgcca gataatcacc tttaccgtga attatcgccc ctgtggtcac catttcggta
    12601 taaagcaacg tattgcggga aagcagacgc aagaaatagc ggcaatgtct gtccgtccag
    12661 tcgagcattg gtgcaacact aaaccgacca ctccagtgaa cgtcagtttt ttcaggcatt
    12721 gcgctggttt ggttgatttt ttgcatttca gaattaccgt gcattttcaa atgtagagat
    12781 tattttatcg atatatcatg gggttatgtt attcagcttc actgttcagg aggctcaata
    12841 gcggggtact ataccataac aacaggaagc gcctgtctca ttgcaaaaga aaattgagat
    12901 cctctcaagg catgaagctc tcacgaagtg atggaaataa tcttattagc cgttaacttt
    12961 gttaaggcca atgataaaca atccaggttc gacgataaat aaaaaatcac acattaaact
    13021 ctggtgatat atctccctgc taatagcatt gatagagaaa aaagaaccca ataaatattg
    13081 ggtcctttta tataatgcct tccatactat cgaagaactt cacatattat ttctccgatt
    13141 taaccccgaa caagtcataa attaatttag aagtgtctgt aagtatttta atctcttcct
    13201 ttgaggttgg gtcaaacgac ttcgcgaagc taattaattg tggtgcagca tctcgcattt
    13261 tgcttaaaat atcaggatcg agcgttcctt cattcaccag atgtgacatc ttatccagat
    13321 agccatcaaa attcatttct cccccatctg ccagacgctt catctctccc aggtacttct
    13381 tcatatcacg ttgatataat ttttcaaact gagctttcag gtagctctca ttattttcat
    13441 taatatacac tgtttctgat aaatctgcgt aggcactaga atatgaaagt gaaattaaca
    13501 gcgaagcaaa taattttaag ctgttgtcat catgttcctg gcaggcatta acaaacgtta
    13561 aaaaaccaga acctattgct tgaaaatgta tattcgctaa aggttcatta tccttagatt
    13621 cctcataaaa tagctgaacc gtggagggaa ggccattctt agtttctaca tcaaaagtac
    13681 attgttcaat gggcattgca tctttattat ccccaggatt acatatacta ttaatcgcga
    13741 aatgaaaaat agcccggtca acaactgatg caataaagtc acgatcgttt aaatcctcat
    13801 cagtgcaatc gtcagtatac ttattgaccc acattttgag atcatttatg tgctcaatta
    13861 aaaacacatt tttttacgtg catgccataa attatgcgtg attcgttgaa taaacaaatc
    13921 tatttttgca ataattatat cccgatgacg cagcgctgca taatcaatag atttaccata
    13981 tatattattt aatttacctt tgattgaacc tctattatcc aatttatctt tggttgcatc
    14041 tttattttcg aattgatttg aattgagtgt tttattaaag tatccagttg caccgggtat
    14101 tatcttcaac atgattcatc tacctttttt gtcgtaccgt gtcctcctta taattctttt
    14161 gccatctggt cactatcaaa aatcgctcat aatttctgaa ctacaggtat gcgtttaaac
    14221 ccagttaaat aaatagcgag ttaacttgaa gcaatttatt taaaaatgaa ttattaatta
    14281 ttatcgtgtt gataatggta cgataaggca acacaacacc ttacctacaa aacagaaaat
    14341 caacattacg gcaacaataa gggttctcgg tgtttttcct ggaacatggt aaagtaagga
    14401 cattcttaac ccccactttg aggtgcccga tggaaaagac cacaacgcag gagttattag
    14461 cgcaggctga aaaaatctgc gcgcagcgta atgtgcgcct gaccccacag cgcctggaag
    14521 tgttgcgcct gatgagtctg caggatggcg ctatcagcgc ttatgatctg cttgatttac
    14581 tgcgcgaagc tgaaccgcaa gccaagccgc caacggttta tcgcgcgctg gattttctgc
    14641 ttgagcaagg ttttgtgcat aaggtggaat ccaccaacag ttatgtgctc tgtcatctgt
    14701 tcgaccagcc cacgcatacg tcagccatgt ttatttgcga tcgctgcggc gcggtgaaag
    14761 aagagtgtgc agaaggcgtg gaagacatta tgcatacgct ggcggcaaaa atggggtttg
    14821 ccctgcggca taatgtgatt gaagcacatg ggctctgtgc ggcatgcgtg gaagtggaag
    14881 cgtgtcgtca tcctgaacag tgccagcatg atcactctgt gcaggtgaaa aagaaaccgc
    14941 gttaagaggg cgtacatcct tgtacacgtc gggcaggagg gattaattac cagcgatatt
    15001 cattgcgggt ttcccaatcc acgacctctt tttctgcctg atctttctga taaccataac
    15061 gttcctggat tttaccgacc agttgatcac gtttaccttc aatgatcgtc atatcatcat
    15121 cggtcagttt gccccattgc tctttcactt tacctttaaa ctgtttccag ttaccgccgg
    15181 cttcatcttt attcataatc aagacctcat cgttaggttg tgaatgagag tacgttcact
    15241 tttcttctga acgtgagatt aagtgtagtc agcaatttgg cttaggataa tttattcaga
    15301 atttttaacc gtcacgttgc ggcaaaccag gtaccgttgc gccagtgacg ccgccagata
    15361 gccgccagag aaagcccgcg caacgccaga aagacggtta atgccagcca caaaccatga
    15421 ttacccagcc acggcagcgt aaggagcgtc agcgcaaaac ctgcggcggc caccgccata
    15481 ctgttacgca tttcggcggc acgcgttgcg cctataaaca tgccgtccag cagataacac
    15541 cagacgccaa ccaacggcaa aatcacctgc cagataagat agcggtcagc cagctgctga
    15601 atctgggtta acgacgtcag caacgcaatg atgtgttccc ctgccagcaa ataaaccacc
    15661 gaaaatagta acgctacaat ccccgactgg cggcacgctg cccgccatac atccagcaac
    15721 tggctaccgt cgcgcgcacc gtatgcctga ccggagtgcg cttcaaccgc gtaggcaaaa
    15781 ccatcaagcg cataggcggt aaaggtgagt agcgtcatca gaaccgcgtt aacagcgata
    15841 atgtcactcc ccagtcgcgc accaagtacg gtgatcgcgc cgaaacagag ttgcaacaac
    15901 agcgaacgca gcatgatatc gcggttaagc gccagcaagc gacggaagtt tcctcgccag
    15961 gcagttttca gcatttcgcc ggaaattccg cgtagtttga ggattttacg caccattagc
    16021 agaccaatca gcaatgttgc atattccgca ataaccgtcg ccagcgccgc gccctgcacg
    16081 ttcatatgca gccccatcac cagccagaca tccagcacaa tgttgaggat attgccgacc
    16141 actaacaaaa ttactggcgc acgggcatat tgcacgccga gtaaccaacc gagtaatacc
    16201 agattcgcca gcgacgccgg agcgcttaac cagcggattt caagaaagcg ccgcgcctgt
    16261 tccaggaccg cttcactacc gccaacaata tgcagcgcca gatcgataat cggcgtacgc
    16321 aacagcgcaa ttaacgcccc ggcccccaac gccaacagca acggttgcac cagcgcacgg
    16381 gctaatgcct gaggattttt ggcaccataa gcctgcgcag tcagcccggt ggtgctcatg
    16441 cgtaaaaaca gcaacagcat aaagagaaag ctggtcgccg ttgcaccaac cgccacgccg
    16501 cccaaataaa ccggactatc aagatgacca attaccgccg tatcgaccag tcccagcaac
    16561 ggaacggtga tattggagaa aatcatgggt aaggcgagat gccagagtgc tttatcagat
    16621 gaagtgagga atgccatgca gacaagcctg atgaagagag atgaaaaaca aaccgcgacg
    16681 ccaagcggca tcgcggtctc agagatatgt tacagccagt cgccgttgcg aataacccca
    16741 accgccagcc cttcaatggt gaagctctgc tgacgaaggt caacgacaat tggtttaaac
    16801 tcgctatttt ctggcaacag ttcgacttta ttgccctgtt ttttcaggcg cttaacggta
    16861 acttcgtcat caatacgtgc gacaacgacc tgaccgttac gtacatcctg agttttatgc
    16921 actgccagca agtcgccatc cataatgccg atatctttca tcgacatccc gctgacgcgc
    16981 agcaggaaat cagcattcgg cttgaacaag gaaggatcga cctgataatg accttcaata
    17041 tgctgttgcg ccagaagcgg ttcaccggca gccacacgac ctaccagcgg caacccttct
    17101 tcctcttcct gcaacagacg aatcccgcgt gatgcgccgg aaacaatttc aataacgcct
    17161 ttgcgtgcca gcgccttcag atgttcttca gccgcgtttg gggaacggaa ccccaaacgc
    17221 tgcgcgattt ccgcacgcgt cggcggcata cctgtctggc tgatgtgatc acggatgaga
    17281 tcaaacacct cttgttgcct ggccgttaac gctttcattc cgccccctgg gtgtatatac
    17341 agttatactg tgagtatata cagcaaaagg cgattttgga accataaact gcacaataaa
    17401 ccagagattt atcgaaatct ggaagggtta tccaaaatgc gaccataaca ggatgcacca
    17461 ggtaatcacg gcgacgataa tggcaatcag caccgcggcg gatcccatat cttttgcgcg
    17521 tccggaaagc tcatggtatt cagagccaat tcggtcaacc actgcttcga tggcgctatt
    17581 gaggatttcc acaatcatca ccagcatcac ggagctgata agcagcacgc gggtaattgc
    17641 gtccacatcc agccagcagg cgatgaccac cgccaacaat accgctacgc cttcctgacg
    17701 gaatgccgct tcgttgatcc atgcagcgcg taaacctttc caggaatagc cagcagcttt
    17761 gataattcgg gtgaatccag ttgtattatt ggccattgaa agaacctttt tacattatga
    17821 gcgtcaatat cagtgtaccg ttaaatccga tgattcgggt ttgcgtaacg ccgcgcgaaa
    17881 catgagcgga taccacagaa tttcccatga ctttctgcta tccttgccgc gcatttgcat
    17941 tattaaccag aggctttaca tcgtttatgt ccggctggcc acgaatttac tacaaattac
    18001 tgaatttacc attaagcatc ctggtaaaaa gcaagtctat tccggcagat cctgccccgg
    18061 aactggggct ggatacctct cgtccaatta tgtacgtttt accgtacaac tcgaaagcag
    18121 atttgctgac gttgcgcgcc cagtgtctgg cacatgattt gcctgacccg ttagagccgc
    18181 tggaaatcga cggcacgcta ctgccgcgct atgtgttcat tcacggcggg ccgcgtgtat
    18241 tcacctatta cacgccgaaa gaagagtcta ttaagctttt ccacgactat ctcgatttgc
    18301 accgtagcaa cccaaatctg gatgtgcaga tggtgccagt atcggtgatg tttggtcgcg
    18361 cgccggggcg tgaaaaaggc gaagtgaacc cgccgctgcg tatgcttaac ggcgtacaga
    18421 aatttttcgc tgtactgtgg ctcggtcgcg acagttttgt gcgtttctcg ccgtcagtgt
    18481 cgctgcgccg tatggcggat gaacacggca cggataaaac tatcgctcag aaactggcgc
    18541 gcgtggcgcg tatgcacttt gcccgtcaac gtctggctgc cgtaggccca cgtctgcctg
    18601 ctcgtcagga tctgtttaat aagctgctcg cctcccgcgc cattgccaaa gcggtagaag
    18661 atgaagcgcg cagcaaaaaa atctcccatg aaaaagcgca gcagaacgcg attgcgctga
    18721 tggaagagat tgcggcgaat ttctcttacg agatgatccg cctgactgac cgcattctgg
    18781 gcttcacctg gaaccgattt tatcagggca tcaacgtcca taacgccgag cgcgttcgcc
    18841 agctggccca cgacggtcat gagctggtgt atgtgccttg ccaccgcagt cacatggact
    18901 acctgctact ttcttacgtg ctgtaccacc agggactggt gccgccgcat atcgccgccg
    18961 ggatcaacct gaacttctgg ccggccgggc cgattttccg ccgtctgggg gcgttcttta
    19021 ttcgccgtac ttttaaaggc aataaacttt attccaccgt tttccgtgag tatctcggcg
    19081 aactgttcag ccgtggttat tccgtcgagt acttcgtgga aggcggtcgt tcccgcacgg
    19141 ggcgtttgct ggatccgaaa accggtacgc tgtcgatgac cattcaggcg atgctgcgtg
    19201 gcggcacgcg tccgattacg ctgattccga tctatatcgg ttatgagcac gtcatggaag
    19261 tgggtactta cgccaaagaa ctgcgcggcg cgacgaaaga gaaagagagc ctgccgcaga
    19321 tgctgcgcgg tttaagcaag ctgcgtaatc tcggtcaggg ttacgtcaac ttcggtgaac
    19381 caatgccgtt gatgacctac cttaaccagc acgtaccaga ctggcgtgaa tctatcgatc
    19441 ccatcgaagc ggtgcgtccg gcctggttaa cgccgacggt caataatatt gctgccgatc
    19501 tgatggtacg cattaacaac gcaggcgcgg caaacgccat gaacctgtgc tgtactgcgc
    19561 tactggcatc acgtcagcgc tcactcaccc gcgagcagtt aaccgagcaa ctcaactgct
    19621 acctggatct gatgcgcaac gtaccttact ccacggactc taccgttcct tcagccagcg
    19681 ccagcgagct tatcgatcac gcgctgcaaa tgaacaagtt tgaagtcgag aaagacacta
    19741 tcggcgacat catcattctg ccacgcgagc aagcggtgct gatgacctac tatcgcaaca
    19801 acattgcgca tatgttggtg ctgccttcgc tgatggcggc aattgtcacc cagcatcgcc
    19861 acatctcccg cgacgtattg atggagcacg tcaatgtgct ttacccaatg ctgaaagcgg
    19921 agctgttcct gcgctgggat cgcgacgagt tgccggacgt tattgatgcg ctggcaaatg
    19981 agatgcaacg tcaggggctg attaccctgc aagatgatga gttgcatatc aacccggcgc
    20041 attctcgcac cctacagctg ctggccgcag gcgcgcgcga aacgctgcaa cgttatgcca
    20101 tcaccttctg gttgttgagt gccaacccgt cgatcaaccg cggtacgctg gagaaagaga
    20161 gccgcaccgt tgcgcaacgt ctctccgtgc tgcacggcat caacgcgccg gagttcttcg
    20221 acaaggcggt gttcagttct ctggtgctga cgctgcgtga tgaagggtat atcagcgata
    20281 gcggcgatgc cgaaccagca gaaacgatga aggtttatca gttgctggcg gagttgatta
    20341 catcagacgt gcgtttgacg attgagagtg cgacgcaggg cgaagggtaa tcagagagaa
    20401 ttgccggatg cggcgaaaac gccttatccg gcctaccatg acctgcaaat tcaataaatt
    20461 gcgattcacc gggtaggcct gagaagcgca acgcatcagg caacccagaa gaaagccgga
    20521 tgatcatccg gcttttttac atcatcagaa atgccagtaa ctcattgcca gtcctaaaaa
    20581 cagtaccaga ccaacatagt tattattcat aaatgcttta aagcaggctt cacgctcgcg
    20641 gttggcaatc agtttttgtt gataaacaaa cagcgcgcca gccaccacaa ttgaccagta
    20701 atatccccag cctaagccat ttaactcacc gatgatcgcc atcagtgcca gtacgccaat
    20761 ctgcaaaata ccaataatca atttatcgta ttggccgaac aggattgccg tggatttaat
    20821 gccaatcttc acatcatcat cgcggtcaac catcgcatac tgcgtgtcgt aagccaccgc
    20881 ccagagaata ttggcgagga acattaacca gcaactcaat ggcaccgact cactcacagc
    20941 ggcaaaagcc attggaatcg accagccaaa cgccgcgccc agcaccactt gcgggagatg
    21001 ggtataccgc ttcataaacg ggtacaccca cgccaatgcc agagcggcaa tcgacaacag
    21061 aatggtcatc gtattcagcg tcagaaccag taaaaacgaa atcagtacca gcactacaaa
    21121 cagcgcgcgc gcctctttct ctgttaccgc gccgctggga agtggtcggt tcgccgtgcg
    21181 cttaacatga ccatcaaact tgcgatcagc ataatcattc accacgcaac cagcagcgcg
    21241 catcagccag acacccgcga caaacaccgc catgatccag agctgaggaa cgcccggtgt
    21301 cgccacccac aacgcccata atgttggcca gagcagcagt aacgcgccaa ttggcttatc
    21361 cgtacgcatt aagcgatgaa acgccagcag cttattctgc gtcagactcc actccatatt
    21421 tttttcctct tagtacaacg gtgacgccgg taaaaacagt tctgttagca acagcggttt
    21481 accgcttaat cgcaggcggg aacgtcgccc ccacagtccg gcatcacggc ctatctcgat
    21541 aaaatcccgg gttagtgtcg atgatgtgaa cagatagcgt cctaacggcg ttttacccaa
    21601 tttttgtaac gctagttccg gcccgcttaa tgttgataca ggaacgacgg tacgaccggc
    21661 aagccacggt tcaccatcgg cacataacaa aatttcacgt aaccagtaac gagactcttt
    21721 cggcagcagc ggcagttctt cggggatttc attctgctcg acaaacccct cgcggatcat
    21781 cgtcacactc accgtttttc cctgctgttc aaaacgtttt gtcatggaat cctccagcaa
    21841 cagccagtcg agcagttgcg gatccagggc agggatctct ttaaaatagc gcagcgcacg
    21901 cagttgcgtt aacgcggggt gtgacatgcc gaactctccg ttacataacg taaaggcatt
    21961 gtatcgcaga aaagtgttta cgggagatgt aaagcaaatt aagtgatcta atgcgcaaca
    22021 aaaacgcaac aatcgcatga agtggtaaaa cgctgttgaa ccttacgctt tgccgatgcc
    22081 cgtgctggcg agggcaacca taggggcatc accgcccatg atgctaggaa cggtgatgtt
    22141 ttcctttact taaatacctt tattctttat acgagtagct cgccaactcg cagtaaaaat
    22201 tgggaaaagc gcatatttac catgagattt ggagctgaaa agtgtttcct gagtaagatt
    22261 aataggcaac atcttatgtc tggatttata gatacacgtg gaattccaat tggtatcttt
    22321 tgtaggattc aacatatcat gtaagcgctg ggagcttacc accataacag gcccatcatc
    22381 gacagaggag aacaggataa atatattatc gtttcgtgtt aatttattag tgttaaaggt
    22441 tcttaacttt tcttcagttt ctataaatat aggtgaaaca tcatcactac gaatccactc
    22501 tttataaaga taccaggcat ttgctttgat catatcatcc gttgatcgta aggcctgatc
    22561 gataagttgg ataaaaaaat tgttattttt atcaaccagc cctttattca tattaaatct
    22621 gttaataaaa tggatcatct gttgggtatc gaaattcacc ttactgaatc gatgttgacg
    22681 cataaaatta tcgaacttgt catcttcccc agaatgtata taactacgta taattggatc
    22741 ttttatataa ggacaatggc taaatgtttt cttcatattc tcaataaagt ttttattaac
    22801 aatattgaga atttttaatt gcatcaccag ttttactgcc agaacggatt tcaatatcat
    22861 tgcattatct ggcaatgtat caatcatttt tataatgtta ttgatataac catcgagatc
    22921 ttcattccca aatatatctg aataatcaat agttaattcc aattgttctg taatgcaatc
    22981 ttaaaaattc cctgtcgaca ttcgcacatg atcaaacata acagctgtca aattcgattt
    23041 aataaatgat atttctttgg cagacagaaa ctctgctctg gtgaagtgcg aagcgtaaag
    23101 ataacaatta tcgaagtttg aatttctaat ctgacattcg cgaaaattac tatgaccaag
    23161 ttgactctgc gagaagtcaa ctgtatcaag aatattttgg ctaaaatcaa cacattcgcc
    23221 actcgttttg agaaaaatca cattgtgtaa acgtcgacat ttaaatatga cattatcaac
    23281 tgttgcctta gtaaaattgc aattctcaat ttctttacat tcgaaagtta catgttgtag
    23341 atttgatgat aaaaaagaca ccttagaaat aacgtcacct ttaaaatgac aatttgttaa
    23401 atcaaaatca gacaggtcga tatacccctg cgcatcttta tccatcagaa aattacaagc
    23461 gcattctatt ttttcatttc tgtagaattt tacacttata gtatcattac taattgagac
    23521 ttcacgtaac atatttattg catgattttt ttcgttccgt tgttttatat ttaaaaatat
    23581 atcactgata taaataaagt aatctctgtt taaacctgtc accccattga aattacgtag
    23641 taaatcaaga tctactatat tatttccttg caccacatca tgtttcatca aagagttatt
    23701 gccgccagaa tttgtgatat gacctatcat aaaacctctg taatacagaa ataaaataac
    23761 ctggaaaagt agcctgagcg tccagacact accggcgaaa ggaaaagaat ctctgataat
    23821 gcattgaaat aatggacatt cttagtgaag aagaagtgaa gtactataaa aaatcgctca
    23881 tatcccagga aggaagcata aaaaaaggtg cgccaggaga cgcaccagtt gttgcaaaat
    23941 cagcactgtg gggcgtaatt accccttgcc ttttacactg ctgataaagg tggaacgggc
    24001 agatgtcgat cccaggcgtt cagcttcatc aagcagtttt aacgccttat caacatcacc
    24061 tttcgcgaca gcgtttttga tcgcggtatt aaaataactt tccgtgtcgt tgagcatcgg
    24121 ctcgcttttc ttcaccggca ccggagcagg tgcagccaca gctggtgccg ccgtgttgcc
    24181 taccgtaacc ggagctggag cggaagaacc aaatagcggt cctaccaaca cgctggagct
    24241 ggagttcgtt ttgactttca gtttcagtaa gccatcgctg gtatgacgag caaccggatc
    24301 gggaatatcc gggatcgagt taccgacgcc tttggcatag gctttagccg ggtcgagcag
    24361 ttgggtcgtc tgctggagat ctttttccgt ggtaaagacc agaacataaa gtttttgctg
    24421 ccccaacgcc ggtgtcaggc gcataacgcc ttccagccga tctgcactca tcacgcctag
    24481 ttcctggtag gtgaaataac tgctggggaa gaaggctgat ggggtcatgt tctgatcaag
    24541 aatcagcacg ttcggcgcaa aaacgctggt ttgtttgttc acttcgctgg tcagcgtcag
    24601 ggtcagttcg ccaatgtttg ccgggacgct gtacgcagca accggaccac tgatgccggg
    24661 aacgttcagt tgttggccgc cggtcgccag ttgggtggtc tgggttttag attgatcgac
    24721 cggtgtccag gtgagttgtt gcagcgcggc agatggaatg gctggcgcgt cgctggtgtt
    24781 ttgcggtacg tagttaacat cggcaaggct aattccaggc gcgcttgcca gtaaccctgc
    24841 tgataaacag agggcgatga gacttttatt cattttcatt gttttcacct caaaatctgg
    24901 agctcagcgg tagccaggca atagcgcgct aaacccgata atcagagggg ctttcgcccc
    24961 tttcagataa tgacaacctg tttttatgcc ggatgcggcg taaacgcctt atccggccca
    25021 ggttttgcta ttaccaccag atttccatct gggcaccgaa ggtccactcg tcgctgtcgc
    25081 cacgaccgaa gctgccgccg ttgaaatcag caggaacggc tttgccgtag ttcgggttaa
    25141 ccttgctatc gccgttgtag tcgtaacccc atttctcatc ccacttggcg taggttgcga
    25201 agacacgaat agccgggcgt gaccagatgc tgtcgccagc ctgccattgt tgtgcgaggg
    25261 taattttgta ctgattgttc ttgtcgccgg tgcgctggga ttcgacgttg tcgtagccga
    25321 tttccatcac ggtgctcatg attggcgtcc acttgtacat cgggcgaata ccaacggtcc
    25381 accacttggt gccgttgtcg ttatcccagt tgatatcctg gtacataccc acgtacatca
    25441 tgtcccagtt gtcgcccatg gagatcgcac cgtggtcgag gatacgcagc atgtgaccgt
    25501 tgttgttgat attgtaggca aatttttcgt tatcaaacgc gacgccagaa ccctgcgaca
    25561 gacctttacc ctgcgaggtc atcgagtcag tagcgtactg aacaacaaac ttgttaaagc
    25621 ccttcaggac actctgagta tgttcagcag tgaataacca gccgtctttc gatgcgccat
    25681 caaccagacg atagttatca cgcaggttgg cacgaccgta gtcgacaccc agttctaatg
    25741 tgccgcccgg gttgatttcc atctgcgcta aacgcacatc gaaaacgtcg ttcgcggttt
    25801 cgttggtata gtcataaata ttgttgctgg caaaagagga agaaccacca gcttcagagg
    25861 agcgggttgc tgccagagag agtttaccga agccaacatc gatgttttcc agaccggcac
    25921 caggaccaga aatatcccag tagtagaagt cgatcatatg aacgtcatga cgttggtaga
    25981 agcgcttacc tgcccagatg gtggaacctg gcagccattc gatcaggttt tttccctgca
    26041 cgtttgcttc acggaaggcc ggatcggtag cttcccagtc attctgttgc gcgacggaat
    26101 aggccacgtt agtgtcgaaa tagaagctct tatcgccctc tttccacact tcctgaccca
    26161 attttaattc agcataagtt tcacattcgt tgccaagacg gtatttactt tgagcaccgg
    26221 tagtctggaa acactgttgt tcaccgccgc tacctgtcca gccaataccg gaacgtgcat
    26281 agccgtggaa atcaacagcc attgcctgag cagacattac gcccgctgcg acggcaaccg
    26341 ccagaggaag tttgcgcaga gtaatcatca ttctatctcc tgagtcattg cttttctttt
    26401 ttcacatcac ctgtgacagg ctttgtgtgt tttgtggggt gcttaaacgc ccggctcctt
    26461 atgcagtcga cgacatgcag tgccatcctc acggaacaga tggcaacgct ctggcggcag
    26521 gccgatagcg aatgtggcac cttcttctac caacaccacg tcgttctggc ggtacaccag
    26581 gttttgacga atggaaggga tctggatata gatttgggtt tcgttgccga gttgctcgac
    26641 gacctgaact tcaccctcaa ggatgacgtc agcgatatca ctcggcagta gatgttccgg
    26701 gcgaataccc agcgacatat tggctccaac ctggacatca cggctttcaa caggcagcca
    26761 gacttgttga cgatttggca tcggcagctc cacctgcact tgatcgattg cggtggcggt
    26821 cacttttacc ggcaggaagt tcatctttgg cgaaccgata aatccggcga caaaacggtc
    26881 tgccggatag tggtacagtt ccagcggttt cccaacctgt gccacgcgac cggcgtccag
    26941 caccacgatt ttgtcggcca gcgtcatcgc ttcgacctga tcgtgggtga cgtaaatcat
    27001 tgtgcggccc aggcgtttat gcagacggga gatttcgata cgcatttgca cacgcagtgc
    27061 agcatcgagg ttggagagcg gttcatcgag caaaaatacg cttggctcgg ccaccagcgt
    27121 acggccaatc gccacgcgct gacgctgacc accggagagc gctttcggtt tgcgatccag
    27181 caaatgcgcc agttgcagca cttccgccac ctggttgacg cgttggttaa tcacctcttt
    27241 ttttgcgcca gccagtttca ggccaaatga catgttttct gctactgaca ggtggggata
    27301 gagcgcgtaa gactgaaaca ccataccaac gccgcgttct gctggcggag tgtcattcat
    27361 ccgtttctca ccgatgaaca ggtcgccgct ggtgatcgtc tcaagcccgg caatcatgcg
    27421 cagtaaagtc gatttaccgc agccagacgg tccgacaaac accacgaatt caccttcatg
    27481 gatatcgaga ttgatatctt tcgataccac gacctcgccc caggctttcg ttacattttg
    27541 cagctgtacg ctcgccatgc ccttctccct ttgtaacaac ctgtcatcga cagcaacatt
    27601 catgatgggc tgactatgcg tcatcaggag atggcttaaa tcctccaccc cctggctttt
    27661 ttatggggga ggaggcggga ggatgagaac gcggcttctg tgaactaaac cgaggtcatg
    27721 taaggaattt cgtgatgttg cttgcaaaaa tcgtggcgat tttatgtgcg catccccaca
    27781 ttaccgccaa ttctgtaaca gagatcacac aaagcaacgg tggggcgtag gggcaaggag
    27841 gatggaaaga ggttgccgta taaagaaact agagtccgtt taggtgtttt cacgagcact
    27901 tcaccaacaa ggaccataga ttatgaaaat aaaaacaggt gcacgcatcc tcgcattatc
    27961 cgcattaacg acgatgatgt tttccgcctc ggctctcgcc aaaatcgaag aaggtaaact
    28021 ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt
    28081 cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt
    28141 ccctcaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcgc acgaccgctt
    28201 tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga
    28261 caagctgtat ccgtttacat gggatgccgt acgttacaac ggcaagctga ttgcttaccc
    28321 aatcgctgtt gaagcgttat cgctgattta taacaaagac ctgctgccga acccgccaaa
    28381 aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct
    28441 gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta
    28501 tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg
    28561 cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga
    28621 caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa
    28681 cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact
    28741 gccgaccttc aagggtcaac catctaaacc gttcgttggc gtgctgagca ccggtattaa
    28801 cgccgccagt ccgaacaaag agctggcgaa agagttcctc gaaaactatc tgctgactga
    28861 tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta
    28921 cgaggaagag ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg
    28981 tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg tatgccgtac gtactgcggt
    29041 gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactcg
    29101 tatcaccaag taatgctgtg aaatgccgga tgcggcgtga acgccttgtc cggcctacaa
    29161 aactgaaacg catgtaggcc tgataagacg catcagtgtc gcatcaggca gttgttgtcg
    29221 gataaggcgt gaaagcctta tccgtcctgg aatgaggaag aaccccatgg atgtcattaa
    29281 aaagaaacat tggtggcaaa gcgacgcgct gaaatggtca gtgctaggtc tgctcggcct
    29341 gctggtgggt taccttgttg ttttaatgta cgcacaaggg gaatacctgt tcgccattac
    29401 cacgctgata ttgagttcag cggggctgta tattttcgcc aatcgtaaag cctacgcctg
    29461 gcgctatgtt tacccgggaa tggctggaat gggattattc gtcctcttcc ctctggtctg
    29521 caccatcgcc attgccttca ccaactacag cagcactaac cagctgactt ttgaacgtgc
    29581 gcaggaagtg ttgttagatc gctcctggca agcaggcaaa acctataact ttggtcttta
    29641 cccggcgggc gatgagtggc aactggcgct cagcgacggc gaaaccggca aaaattacct
    29701 ctccgacgcg tttaaatttg gcggcgagca aaaactgcaa ctgaaagaaa cgaccgccca
    29761 gcccgaaggc gaacgcgcga atctgcgcgt gattacccag aatcgtcagg cgctgagtga
    29821 cattaccgcc attctgccgg atggcaacaa agtgatgatg agctccctgc gccagttttc
    29881 tggcacgcag ccgctctaca cactcgacgg tgacggcacg ttgacgaata atcagagtgg
    29941 cgtgaaatat cgtccgaata accaaattgg cttttaccag tccattaccg ccgacggcaa
    30001 ctggggggat gaaaagctaa gccccggtta caccgtgacc accggctgga aaaactttac
    30061 ccgcgtcttt accgacgaag gcattcagaa accgttcctc gccattttcg tctggaccgt
    30121 ggtgttctcg ctgatcactg tctttttaac ggtggcggtc ggcatggttc tggcatgtct
    30181 ggtgcagtgg gaagcgttgc gcggcaaagc ggtctatcgc gtcctgctga ttctgcccta
    30241 cgcggtgcca tcgttcattt caatcttgat tttcaaagga ttgtttaacc agagcttcgg
    30301 tgaaatcaac atgatgttga gcgcgctgtt tggcgtgaag cccgcctggt tcagcgatcc
    30361 gaccaccgct cgcacgatgc tgattatcgt caatacctgg ctgggttatc cgtacatgat
    30421 gatcctctgc atgggcttgc tgaaagcgat tccggacgat ttgtatgaag cctcagcaat
    30481 ggatggcgca ggtccgttcc agaacttctt taagattacg ctgccgctgc tgattaaacc
    30541 gctgacgccg ctgatgatcg ccagcttcgc ctttaacttt aacaacttcg tgctgattca
    30601 gctgttaacc aacggcggcc cggatcgtct tggcacgacc acgccagccg gttataccga
    30661 cctgcttgtt aactacacct accgtatcgc ttttgaaggc ggcgggggtc aggacttcgg
    30721 tctggcggca gcaattgcca cgctgatctt cctgctggtg ggtgcgctgg cgatagtgaa
    30781 cctgaaagcc acgcgaatga agtttgatta agggagataa caaaaatggc aatggtccaa
    30841 ccgaaatcgc aaaaagcacg tttgtttatt actcacctgc tactgctact ttttatcgcg
    30901 gcgattatgt tcccgctgct gatggtcgtc gctatctcgc tgcgtcaggg aaacttcgcg
    30961 accggtagcc tgatcccgga gcaaatctcc tgggatcact ggaaactggc gttaggtttt
    31021 agcgttgaac aggctgatgg tcgcattacg ccaccgccat tcccggtact gctgtggctg
    31081 tggaactcgg taaaggtcgc tgggatttcc gcgattggca ttgtggcgct ctccaccacc
    31141 tgcgcctacg ctttcgcccg tatgcgcttt ccaggcaaag cgacgctgct gaaaggaatg
    31201 ctgattttcc agatgttccc ggcagtactt tcactggtcg cgttgtatgc gttgtttgat
    31261 cgtctgggtg agtacattcc attcattggc ctgaatactc acggcggcgt aattttcgcg
    31321 tatctgggtg ggattgcgct gcatgtctgg accatcaaag gctatttcga aaccatcgac
    31381 agttcgctgg aagaagcggc tgcgctggat ggtgcgacac cgtggcaggc cttccgcctt
    31441 gtcctgttgc cgctgtcagt accgattctg gcggtggtat tcatcctgtc gtttatcgct
    31501 gccattactg aagttccggt cgcgtcgctg ttactgcgtg acgtaaacag ctacaccctg
    31561 gccgtgggga tgcagcaata cctcaacccg caaaactacc tgtggggtga ctttgccgcc
    31621 gctgccgtga tgtctgcatt accgatcacc atcgtcttct tgctggctca acgctggctg
    31681 gtcaacggcc tgacggcagg tggtgtgaaa ggttaaagat gttgttctgc caatgttatg
    31741 ccgctgcacc ctcaacttac gttatcccaa cttgtgacta ttattcggcg ctccacggag
    31801 cgcctttttt tctttcgtct gcaatctgaa tcgttcgccg gttaatattc ccatcataga
    31861 acttattatt tttacgttat ttgttttccc acttacgata attctctttc gtgctctgag
    31921 tcacggcaat agtattgttt catcaatttt tgataattat cacaattaag atcacagaaa
    31981 agacattacg taaacgcatt gtaaaaaatg ataattgcct taactgcctg acaattccaa
    32041 catcaatgca ctgataaaag atcagaatgg tctaaggcag gtctgaatga atacccagta
    32101 taattccagt tatatatttt cgattacctt agtcgctaca ttaggtggtt tattatttgg
    32161 ctacgacacc gccgttattt ccggtactgt tgagtcactc aataccgtct ttgttgctcc
    32221 acaaaactta agtgaatccg ctgccaactc cctgttagga ttttgcgtag ccagcgctct
    32281 gattggttgc atcatcggcg gtgccctcgg tggttattgc agtaaccgct tcggccgtcg
    32341 tgattcactt aagatttctg ctgtcctgtt ttttatttct ggtgtaggtt ctgcctggcc
    32401 agaacttggt tttacctcta taaacccgga caacacagta cctatttatc tggcaggtta
    32461 tgtcccggaa tttgttattt atcgtattat tggcggtatt ggcgttggtt tagcctcaat
    32521 gctttcacca atgtatattg cggaactggc tccagctcat attcgcggaa aactggtttc
    32581 atttaaccag tttgcgatta ttttcgggca acttttagtt tactgcgtaa actattttat
    32641 tgcccgttcc ggtgatgcca gctggctgaa tactgacggc aggcgttata tgtttgcctc
    32701 ggaatgtatc cctgcactgc tgttcttaat gctgctgtat accgtgccag aaagtcctcg
    32761 ctggctgatg tcacgcggca agcaagaaca gacggaaagt atcctgcgca aaattatggg
    32821 caacacgctt gcaactcagg cagtacagga aattaaacac tccctggatc atggccgcaa
    32881 aaccggtggt cgtctgctga tgtttggcgt gggcgtgatt gtaatcggcg ttatgctctc
    32941 catcttccag caatttgtcg gcatcaatgt ggtgctgtac tacgcgccgg aagtgttcaa
    33001 aacgctgggg gccagcacgg atatcgcgct gttgcagacc attattgtcg gagttatcaa
    33061 cctcaccttt accgtactgg caattatgac tgtggataaa tttggtcgta agccactgca
    33121 aattatcggc gcactcggaa tggcaatcgg tatgtttagc ctcggtaccg cgttttacac
    33181 tcaggcatcg ggtattgtgg cgctactgtc gatgctgttc tatgttgccg cctttgccat
    33241 gtcctggggt ccggtatgct gggtactgct gtcggaaatc ttcccgaatg ctattcgtgg
    33301 taaagcgctg gcaatcgcgg tggcggccca gtggctggcg aactacttcg tctcctggac
    33361 cttcccgatg atggacaaaa actcctggct ggtggcccat ttccacaacg gtttctccta
    33421 ctggatttac ggttgtatgg gcgttctggc agcactgttt atgtggaaat ttgtcccgga
    33481 aaccaaaggt aaaacccttg aggagctgga agcgctctgg gaaccggaaa cgaagaaaac
    33541 acaacaaact gctacgctgt aatcttcctg tccagcacgc cgcgccattt cggcgtgctg
    33601 tctttttact cccgcttcag ccgtttcgaa ttacacagcc acagggtgat caccagtaac
    33661 aggatcgcag ccgagtagat caacacatcc agtggcgatt tatgatcgac gatgatcaag
    33721 cgcacaatgg cggtgatccc aatatagaca aagtaacgta gcgggaagtg aaaaccggac
    33781 tgaaagtact tcacaatcag cgcgataaat tcgaaataga gaaagtaaac caccagtcct
    33841 tctaccagct catatttgct ggtttgttct ggcgcgaaca gcacatcagc cagatgcacc
    33901 gtttctttgc cgaggaagac aaccaaaatc aggccgaggc acagcaggcc aagattgagt
    33961 acggtctgca aaatggtgga gataaactcc acgcgcggac gagagagtga cgtcatagca
    34021 gtacctcctt cacagtggcg agcttcctgt ataacgcaaa aatgtgacgg agatctatat
    34081 tttttgtgta tgttttgttc aacctggatt tggagcgcgc ctccaccaaa gccggatata
    34141 cctacccgta ggccggataa gccgcgcctg gcggcgcatc cggcaagaca aaccgcacca
    34201 aagccggtaa ttcaaaaatc acccattcag gtgattccca gccaccagca atgcgcgaaa
    34261 aatcgacaat gttcctctgg aacaaatgaa acgtaacctg ataaaggtgg taaaaatgaa
    34321 gccttatttt gctgctttga tgttatcagt ctctgttctt cctgcttatg ctggtccgct
    34381 cggtactgct gataaagccg acctaccgca aagcaatgtc tccagcccaa tgatggccca
    34441 gtcgctcagg caaccagacc tgcaaccgat atcgacggac aggaagacag aatgttttcg
    34501 cctttacaca ccagatcgta aaccgggggt gaactgcgtt cctgatggct caacaggtca
    34561 ttaagaatga gggtgccagt gagtgcgaaa actggcacct ttcccatgac ttagcggaaa
    34621 tttacgctac ggtcgctggt catgtcatac aactggaact tacgcccaag ttgctgacca
    34681 ccgtcacgcg tcagcggcgt ccagctaatc gccgcacggc tgcgtgtcgg gccagacgag
    34741 aagagatcca acggtaccga gacatacacg cctttggtga agtccccttc gccgtactcc
    34801 tctttcgaaa cgttggtgat cgtggcatag ccacccacca ccacgccgct gtcaaagcgt
    34861 ttagcgatct ccagcgtgcc gcctttatcc cctgccagat actgcccgac gctggctttc
    34921 accaacacgt cctgggcgaa agatggcgtc cagtaggcgg tcagatgtcc ggttttcacg
    34981 ctgtagtcgg tgaatttcat catatctttt gcactacgcc agtcgcgctg tttaacgtag
    35041 ttggcatcca gaccaaacgc ccagttactg tccagcggac gatacaacac ttcggccccc
    35101 gcaccgccaa acatggtttc gagataacct ccatagacct gaccgtagaa gccgttgccc
    35161 agatgctgga agtagttggc ttgcaggtta ttcacataga catcgttctg cacatactcg
    35221 cgcacatggg tacgaacgcg cggtaagtgc gagtcctgcg gtggattggt gtagttaaac
    35281 ttgtcgtagt tgttggcaag atttgcaaac aggctgccgg tggtcagtag atggtccgtc
    35341 agccacaaat ctgccgttcc catcacgccc agctgataca tgtaaaagtt ttccgggcca
    35401 ccgaccgact ggttcagcac cggatcgata tggaaatcaa agcgtgattt gtcgatatac
    35461 cagccctgct cggtggactt cggaaccact ggctcgacgc gtttgtgcgc cagcgtcgtt
    35521 tcgtggccca acggctctcc ggcgagatga tttttcaggc tggcgacatc ggtttccgtc
    35581 gtcacttgcg gcatgttaag gcgattttcc gtaatgcgga tcgttttgat cccatccggc
    35641 agatcgttca tcacgatccg attagcacgg atgatccctt cgcgcgaatc acgatatttc
    35701 acctgctcgc cggtaacata cagcgtatcg cctttcgcct ggatctgcgg atcggcaagt
    35761 ccggcgttgt atttcagcaa cgttaactga ttcgccacca ccgaatgctg caaaatggca
    35821 tcctgcggct gcggttgata ttgcgggcgg gcgttatcga tgtaggacgg gcgcagatcg
    35881 ttaaagttgg tgcgcagcgt gacgccaaac ataaaggtgt tgccacgttc atagctaagg
    35941 ttaacgtcgg cccaatcggt aacgcgataa atcgccccga cgttaaactt acttttttgc
    36001 tccagcttcc cggcaaaatc ctgctgataa ttattacctt catactccag tttcagacgc
    36061 agcggttgcc agggcgtctg gtattccacg ccgccaaaca gtgaggcagg accgtggaac
    36121 atctggctac cgtcgataga tcccgcctgt ttgtagctgt tatcgcgata gcaatattta
    36181 tcactggctg aacagagcgg attttttaca ttaccgctgg tgcccagata tccccagccc
    36241 aggccgagcg taaaatcgaa cggcccccag gctttgctgg caacaagata ttccgcatca
    36301 aacagccccg taccgccgat atcccgcgca ccaaccgcca cttgcggcag ccagtaactc
    36361 tcttcccaca aacgcagttt gagatcgaag gctttatctt tatacgtttg atcgccagag
    36421 aacgcttcga cgctgctgta ctgccgggtg cgcacgtcgg tgtagcgcag cgttgtttcc
    36481 agccacggga agagttgcac tgaagctgag taataacggt actgatcgtt atcgcgatag
    36541 ttcagactca actccccttc ccgtgccata cgcgcggtgg gagtttgtaa taatcctacg
    36601 ccaccgaaat ccgactgcga cggaccaatg ggcgcaggat atgtttcgcc gtagcaagct
    36661 gtgctaatgc ccagcgccag taagctaagc agatgtcttt ttttcattat tggggtatcc
    36721 gctgcgtcag agtctgaaga atgtcggcgt taagggcatc aggcgtctca ctccagacgg
    36781 aatctgcgag gccaacataa ataatgctgc cgggcatcgg ctctacgtga cgcttgttcc
    36841 agtaagccac cggcgctttt tgcgtgcgtc cgtccgggta aacgacccac gcgtagctgc
    36901 gatccgcacc gctgagcagg ctctggtcag agagatagct cgccacgtcg cgacctggag
    36961 tgaatggctg attgccagga tggctgataa gcccgaacaa cgtgaccgtg gacggcggtg
    37021 gtccgaccca cagcgtgtag ttgccctgca acggcgggtt accgcgttcg gcaacgcgga
    37081 cgatatcggg atcaagattg attttttgcc tgcctgtcac tttcaatgcc tggatttgct
    37141 ggcgtaaggc gttaatggcg gcggcatcgt cggtgctgga atctgcgccc tgttctgcca
    37201 gccgtgtcag caacgcctgc tgctgacgca atgctgccgc cgttgccagc tcttcgctaa
    37261 tcaccgcacc aggccaccag ctgtttgcca gtcgaggttg ccctaccaga tcgattaaat
    37321 gctctgcgcc cgttagcgtt ttggcctcac tgctgccatt gctgaacacc ttgacggttc
    37381 ctgccgcgaa gaccgatgac gctcccacac tcaaaagcaa cgcgacaata gtttgtttaa
    37441 tcattgtttt gccgccttga tgagagtggt tttcaccggg aaataatccg cgccgagata
    37501 ctgttccgac tggcgaattt gcccttcgct gtcgatccag tagcggttat gccagcgcgc
    37561 ctggtcggtg gagacttctt cgtccagcac gcgaaccagg gtttcatcgc tgccgacctt
    37621 gacggtatcg gtgccatccc atttgaagac tgagcgtgcg gtggcgtagc gtacctgctg
    37681 gtactcggtc cagcccatcg tgcgcgtcca tgttgcacca tcaacaattt gtgcgggttt
    37741 aatcagcggg tcggcggcaa ggttattcac ttcaatcagg ttgtcgccgc caagcaaagt
    37801 cttcaccaga cgaccatgtt gtgtgacaag agtggcttga tcctgagtga cccatttttg
    37861 ttgtccgtct tcagcgaagg ccagcaccac aaacaattgc ggcccgccat taagctgcat
    37921 gtactggctg gcgtagggca tattttgaat atcatcgtcc gtcagctgta cgcctggcgt
    37981 gccgaacaga ctgtcccaca gtgaattgcc cagctcttta gtcgtggctg aacaggcctg
    38041 taatagcagg cagataagaa tgagtgcagg tcgcttcacg actcttctcc agaggggcag
    38101 aataaccaca ccggagtgtg gttatggtaa atcccttcat acttcaagcc acagatgcaa
    38161 tgactcaaat tattttgggg atacatactt tattactggg tactggtggt cgtggttgtg
    38221 gtggtcccgg tattagaacc atcaccgcca ccggttgccg cgagcgcgac acccaccgcc
    38281 gagcttacgg tgctgacgct ggtcgcggag gaactccccg ccgaaaccga cgttgctgcc
    38341 gactctgccg cttcgcccac ctgtacaggt gcagcccacg cagaagtcgc cgcaagcgca
    38401 gatatggcaa aaatgccata cagaactttt ttcataacaa tttcccttca ttgaatgaat
    38461 ggagatttac ccaaaaacat ttcgggcggg atcgagtata cacaactaaa gcatgcgaat
    38521 tacggcgcgg ggaattagcg atggagtttt aggataattg gaatgaatat caaatataag
    38581 tttcaactta tatttaaata acaataaaaa tcataagctt aaaataaaaa acaaaagtaa
    38641 ttaatctgcg tattctccga aataaacaac gattaaaaat ctattttagg attaacctca
    38701 cggtatgatt tccgttaaat cacagacaag gggatttatc tgataaaaca atgccgactt
    38761 attgtcggca ttgtttttaa ggttatttaa ctcctcatac tatgcattaa tgtcgatatt
    38821 ggcatcaggc tccggttgcc ggatgcggcg taaacgcctt atccggccta catatcggca
    38881 tcaggcacgc atgatgatta accgcgccac gctttatagc ggttaatcag accattggtc
    38941 gagctatcgt ggctgctgat ttctttatca tctttcagct ctggcagaat acggtttgcc
    39001 agttgtttac ccagctccac gccccactgg tcgaaggtga agatgttcag gatcacgccc
    39061 tgagtaaaga ttttgtgctc atacagcgca atcaacgcac ccaggctgaa cggagtgatt
    39121 tcgcgcagca ggatggagtt ggtcgggcgg ttaccttcga acactttgaa cggcaccacg
    39181 tagtcaagcg ttgccggatc tttaccctga tcgcgatatt cctgctcaac cacttcgcgg
    39241 gatttaccaa acgccagcgc ttcggtctgg gcgaagaagt tagacagcag tttctggtgg
    39301 tgatcagaga gcgggttatg ggtgatagcc ggggcgatga aatcgcacgg taccattttg
    39361 gttccctggt ggatcagctg gtagaacgcg tgctgaccgt tagtgcctgg ttcaccccag
    39421 ataatcgggc cagtctggta atccacaacg ttaccgttac ggtcaacata cttaccgttg
    39481 gactccatat tgccctgctg gaagtacgcc gcgaaacggt gcatatactg gtcatacggc
    39541 agaatcgctt cagtttccgc accaaagaaa ttgttgtacc agatgccaat cagcgccagc
    39601 aggacaggca ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat tgcgtgtgcg
    39661 ccagaaagca gttcaacgaa gttatcaaag ccgatggaga gaacaatcga caggccaatc
    39721 gctgaccaca aagagtaacg gccgccgacc cagtcccaga actcgaacat gttggcagta
    39781 tcaataccaa actcgccaac ggctttggca ttggttgaga gcgccgcaaa atgtttcgca
    39841 acgtgttttt catcacctgc cgctttcagg aaccagtcac gcgcgctatg ggcgttggtc
    39901 atggtttcct gagtggtgaa ggttttagat gctaccagga acagcgtggt ttccgggttt
    39961 acttttttca gcacttccga gatgtgagtc ccatcgacgt tagaaacaaa gtgcatgttc
    40021 aggtggtttt tgtacggacg cagtgcttcg gtcaccatgt acgggccgag gtcagaaccg
    40081 ccgatcccga tgttcactac gtcagtgatt gctttgccgg tataaccctt ccactcaccg
    40141 gagataatcg cttccgagaa ggttttcatc ttctccagca ccgcgttgac ttctggcata
    40201 acatctttgc catcaaccag aatcggggta ttgctacgat tacgcagcgc tacgtgcagc
    40261 acggcgcggt tttcagtgcg gttgatcttc tcgccagaga acatcgactt aatcgcgccc
    40321 gccagatcgc actctttcgc cagatcctgt aatttcgcca gcgtctcttc agtgatgcgg
    40381 tttttggagt aatccaccag catctgatcg tcgaaggttg cggagaactt agaaaaacga
    40441 tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc gaagtgtttc
    40501 tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt ttttcattag caatactctt
    40561 ctgattttga gaattgtgac tttggaagat tgtagcgcca gtcacagaaa aatgtgatgg
    40621 ttttagtgcc gttagtgtaa tgttgagtgt aaacccttag cgcagtgaag catttattag
    40681 ctgaactact gaccgccagg agtggatgaa aaatccgcat gaccccgtcg ttgacaaccg
    40741 ccccgctcac cctttattta taaatgtact acctgcgcta gcgcaggcca gaagaggcgc
    40801 gttgcccaag taacggtgtt ggaggagcca gtcctgtgat aacacctgag ggggtgcatc
    40861 gccgaggtga ttgaacggct ggccacgttc atcatcggct acaggggctg aatcccctgg
    40921 gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac ttctgggtga
    40981 aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc gctcttccct tgtgccaagg
    41041 ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa attgttgtct ccaaatttgg
    41101 cggtaccagc gtagctgatt ttgacgccat gaaccgcagc gctgatattg tgctttctga
    41161 tgccaacgtg cgtttagtag tcctctcggc ttctgctggt atcactaatc tgctggtcgc
    41221 tttagcggaa gggctggaac ctggcgagcg attcgaaaaa ctcgacgcta ttcgcaacat
    41281 ccagtttgcc attctggaac gtctgcgtta cccgaacgtt atccgtgaag agattgaacg
    41341 tctgctggag aacattactg ttctggcaga agcggcggcg ctggcaacgt ctccggcgct
    41401 gacagatgag ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg tcgaaatcct
    41461 gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt aaagtgatgc gtaccaacga
    41521 ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg gaactggccg cgctgcagct
    41581 gctcccacgc ctcaatgaag gcttagtgat cacccaggga tttatcggta gcgaaaataa
    41641 aggtcgtaca acgacgcttg gccgtggagg cagcgattat acggcagcct tgctggcgga
    41701 ggctttacac gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct acaccaccga
    41761 tccacgcgta gtttccgcag caaaacgcat tgatgaaatc gcgtttgccg aagcggcaga
    41821 gatggcaact tttggtgcaa aagtactgca tccggcaacg ttgctacccg cagtacgcag
    41881 cgatatcccg gtctttgtcg gctccagcaa agacccacgc gcaggtggta cgctggtgtg
    41941 caataaaact gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca atcagactct
    42001 gctcactttg cacagcctga atatgctgca ttctcgcggc ttcctcgcgg aagttttcgg
    42061 catcctcgcg cggcataata tttcggtaga cttaatcacc acgtcagaag tgagcgtggc
    42121 attaaccctt gataccaccg gttcaacctc cactggcgat acgttgctga cgcaatctct
    42181 gctgatggag ctttccgcac tgtgccgggt ggaggtggaa gaaggtctgg cgctggtcgc
    42241 gttgattggc aatgacctgt caaaagcctg cggcgttggc aaagaggtat tcggcgtact
    42301 ggaaccgttc aacattcgca tgatttgtta cggcgcatcc agccataacc tgtgcttcct
    42361 ggtgcccggc gaagatgccg agcaggtggt gcaaaaactg catagtaatt tgtttgagta
    42421 aatactgtat ggcctggaag ctatatttcg ggccgtattg a
//
LOCUS       DAJTYH010000023        40672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTYH010000023 DAJTYH010000000
VERSION     DAJTYH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(74..1327)
                     /gene="amiC"
                     /locus_tag="OO609_001248"
     CDS             complement(74..1327)
                     /gene="amiC"
                     /locus_tag="OO609_001248"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6632739.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1559..2890
                     /gene="argA"
                     /locus_tag="OO609_001249"
     CDS             1559..2890
                     /gene="argA"
                     /locus_tag="OO609_001249"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6632740.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2952..4778)
                     /gene="recD"
                     /locus_tag="OO609_001250"
     CDS             complement(2952..4778)
                     /gene="recD"
                     /locus_tag="OO609_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6632741.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4778..8320)
                     /gene="recB"
                     /locus_tag="OO609_001251"
     CDS             complement(4778..8320)
                     /gene="recB"
                     /locus_tag="OO609_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6632742.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="OO609_001252"
     CDS             complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="OO609_001252"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6632743.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11377..14745)
                     /gene="recC"
                     /locus_tag="OO609_001253"
     CDS             complement(11377..14745)
                     /gene="recC"
                     /locus_tag="OO609_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6632744.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14758..15108)
                     /locus_tag="OO609_001254"
     CDS             complement(14758..15108)
                     /locus_tag="OO609_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6632745.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(15093..15500)
                     /locus_tag="OO609_001255"
     CDS             complement(15093..15500)
                     /locus_tag="OO609_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6632746.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15497..16060)
                     /locus_tag="OO609_001256"
     CDS             complement(15497..16060)
                     /locus_tag="OO609_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6632747.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16051..16521)
                     /locus_tag="OO609_001257"
                     /pseudo
     CDS             complement(16051..16521)
                     /locus_tag="OO609_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16705..17499)
                     /gene="thyA"
                     /locus_tag="OO609_001258"
     CDS             complement(16705..17499)
                     /gene="thyA"
                     /locus_tag="OO609_001258"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6632748.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17506..18381)
                     /gene="lgt"
                     /locus_tag="OO609_001259"
     CDS             complement(17506..18381)
                     /gene="lgt"
                     /locus_tag="OO609_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6632749.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18532..20778)
                     /gene="ptsP"
                     /locus_tag="OO609_001260"
     CDS             complement(18532..20778)
                     /gene="ptsP"
                     /locus_tag="OO609_001260"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6632750.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20791..21321)
                     /gene="rppH"
                     /locus_tag="OO609_001261"
     CDS             complement(20791..21321)
                     /gene="rppH"
                     /locus_tag="OO609_001261"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6632751.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22006..22695
                     /gene="mutH"
                     /locus_tag="OO609_001262"
     CDS             22006..22695
                     /gene="mutH"
                     /locus_tag="OO609_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6632752.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22764..23477
                     /locus_tag="OO609_001263"
     CDS             22764..23477
                     /locus_tag="OO609_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6632753.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23615..23833
                     /gene="ygdR"
                     /locus_tag="OO609_001264"
     CDS             23615..23833
                     /gene="ygdR"
                     /locus_tag="OO609_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6632754.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23941..24981
                     /locus_tag="OO609_001265"
     CDS             23941..24981
                     /locus_tag="OO609_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6632755.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25014..26204)
                     /gene="lplT"
                     /locus_tag="OO609_001266"
     CDS             complement(25014..26204)
                     /gene="lplT"
                     /locus_tag="OO609_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6632756.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26197..28356)
                     /gene="aas"
                     /locus_tag="OO609_001267"
     CDS             complement(26197..28356)
                     /gene="aas"
                     /locus_tag="OO609_001267"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6632757.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28942..29973
                     /gene="galR"
                     /locus_tag="OO609_001268"
     CDS             28942..29973
                     /gene="galR"
                     /locus_tag="OO609_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6632758.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29980..31242)
                     /gene="lysA"
                     /locus_tag="OO609_001269"
     CDS             complement(29980..31242)
                     /gene="lysA"
                     /locus_tag="OO609_001269"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6632759.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31364..32299
                     /gene="lysR"
                     /locus_tag="OO609_001270"
     CDS             31364..32299
                     /gene="lysR"
                     /locus_tag="OO609_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6632760.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32286..32978)
                     /gene="ygeA"
                     /locus_tag="OO609_001271"
     CDS             complement(32286..32978)
                     /gene="ygeA"
                     /locus_tag="OO609_001271"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6632761.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33107..34525)
                     /gene="araE"
                     /locus_tag="OO609_001272"
     CDS             complement(33107..34525)
                     /gene="araE"
                     /locus_tag="OO609_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6632762.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34840..35601)
                     /gene="kduD"
                     /locus_tag="OO609_001273"
     CDS             complement(34840..35601)
                     /gene="kduD"
                     /locus_tag="OO609_001273"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6632763.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35631..36467)
                     /gene="kduI"
                     /locus_tag="OO609_001274"
     CDS             complement(35631..36467)
                     /gene="kduI"
                     /locus_tag="OO609_001274"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6632764.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36754..37935)
                     /locus_tag="OO609_001275"
     CDS             complement(36754..37935)
                     /locus_tag="OO609_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6632765.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38190..39419
                     /locus_tag="OO609_001276"
                     /pseudo
     CDS             38190..39419
                     /locus_tag="OO609_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            40054..40672
                     /locus_tag="OO609_001277"
                     /pseudo
     CDS             40054..40672
                     /locus_tag="OO609_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT        10105 a        11004 c         9882 g         9681 t
ORIGIN      
        1 ttattgaaca ccctaacggg tgtttttttg tttctgatct cccataaaaa aagcgccatt
       61 cagcgccttt ttatcatccc cttctcgcca gcgttgcccc atcggcaaaa tacgctttaa
      121 tccccgcaag aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct
      181 cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct
      241 ttagtacggc aaacccggcc tgttcaactt gatttttatg cagcttgttg atttttccga
      301 gcttattcag taccgcttta ccaaacttaa ggctgtcagc aatagtcagc gactgcacca
      361 tatcgaacat ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt
      421 ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgcg cctttggttg
      481 agagcgcaaa caccgaggaa ccgctcggct gacgactggt aaaggcgtcg gcatggatag
      541 agacaaacag gtcagcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga
      601 agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctcttcctcg atcagagagc
      661 gcaaacggcg agctatttgc aataccacat ctttttcgcg cgttttgtat ttccccaccg
      721 caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc
      781 ctgctttacc cggttgtgga ccactttgcg ctggcggcac ctgcttttcg aggtcgcctt
      841 tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat
      901 agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg
      961 gttttacgtt ttgctttaat tcaaaaacca tgcgtacggt ttgcgggtca aattgcccga
     1021 cgcgcgccga cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta
     1081 ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttcagggtta ctcaacgcga
     1141 actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag
     1201 gccagacgcg caccgccacg acctggctaa ccgcagccag gctgacctga cttacgctca
     1261 atagccacat agcgcccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc
     1321 ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt
     1381 gaccggaaac tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt
     1441 ttatattcac ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc
     1501 tgcgaaaaaa cagaataaaa atacaataat ttcaaataat catgcaaagg ggtgtgccgt
     1561 ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa
     1621 tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa
     1681 tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt
     1741 ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct
     1801 gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc
     1861 gggaacattg caactggata ttactgctcg cctgtcaatg agtctcaata acacgccgct
     1921 gcagggcgcg catatcaacg tcgttagtgg caattttatt attgcccagc cgctgggcgt
     1981 cgatgacggc gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgct
     2041 ccatcgtcaa ctggaaagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac
     2101 tggcgagagc tttaatctga cctcggaaga gattgccact caactagcca tcaaactgaa
     2161 agctgaaaag atgattggtt tttgctcttc ccagggcgtt actaatgacg acggtgatat
     2221 tgtctccgaa cttttcccta acgaagcgca agcacgggta gaagcccagg aagagaaagg
     2281 cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg
     2341 cgtgcgccgc tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt
     2401 ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc
     2461 aacaatcaac gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat
     2521 tctggtgcgc cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca
     2581 gcgcgataac acgactattg cctgcgccgc gctctatcca ttcccggaag agaagattgg
     2641 ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct
     2701 gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac
     2761 cacgcgcagt attcactggt tccaggaacg tggatttacc ccggtggata ttgatttact
     2821 gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga
     2881 tttagggtaa gcgatgaaaa tcaccggatg cggcaggcgt aacactcgtg cgtcgcatcc
     2941 ggcaattacg tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta
     3001 cgagtggcga ttgccgcact taatatacgc tcatcggcat acagcgacag acgacgacgc
     3061 gcgcgggtca ccgcggtgta taccagctct cgcgttacta ccggcgtgcg ttggctcggt
     3121 aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac
     3181 gtagtttcgt gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc
     3241 gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac
     3301 aacccaagcg cgctgtcatt acgggcaatc atcaccggtc ggccttcgta ccaacgagag
     3361 tgcggatgac gatgaatttt gcgcttctgt tgcataaact gttcaattcg ctcattcagt
     3421 ccagccacgc caaacggccc ttcccgcagg gcgcacaaaa gctggtactc attgaacgcc
     3481 tgaatgatta aatccggctc ggcacgcgct tgcagcagat ccagataacg tccgtaaccc
     3541 gcaagagctt cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag tcgtttttcg
     3601 atatcagtaa aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc
     3661 gccgcagcta actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg
     3721 cagagactgt cgcgcaaaga tgccgcttct gtgccggttc ctgccggaac gtgagtcccc
     3781 gtcaggcggc ttagctgcct ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag
     3841 atatcgccca gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag
     3901 atcactcgcg catgatctgg caaggcgtcg atcagtctcg acatcatagg cagatcgatc
     3961 attgacgctt catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt
     4021 aaacgctggc tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga
     4081 atgcgtttct tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg
     4141 gttaagcgcg cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg
     4201 tcggccattt gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt accggtgcct
     4261 gggccgccgg aaatcaccga gatccgccgc gtcagcgcca ctgccgctgc aactttttgc
     4321 cagttaattt catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca
     4381 tcaacctcaa tggtatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac
     4441 cacatgcgat tcaaataaag acgatcgcca cagaggatca tcggcgtggg ttcatctccc
     4501 ctgctgaccg cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca
     4561 ctgacacagg tcgccaacag cggattcgac gcctcgttat tttccagtcg tgaaagcggc
     4621 aaacagacgt gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca
     4681 ggatgttcat ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg
     4741 tgctccacag cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac
     4801 cggcaaacat ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc
     4861 cttgttgcgg atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt
     4921 gaagatcata gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat
     4981 aaagctgata ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg
     5041 tgtaagccga actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc
     5101 cttcgtggcg gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact
     5161 ccagcggcgg gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc
     5221 tggcgatgag cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt
     5281 cggaaagctg actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga
     5341 tccactcggt caacaccggt tcccactgcg attcaaagcc gccgagttcc agtttttcct
     5401 gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga
     5461 atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga
     5521 cgctgaccac gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt
     5581 gaccacgctg ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt
     5641 gtagtgtcct cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat
     5701 caccagtttg tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc
     5761 gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt
     5821 ggacgtcggt gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac
     5881 tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac
     5941 gttcgacctc cgcgaggtcg acgctttctg gcgcagcatt aagatccaaa actgcctcaa
     6001 acgagtggcg atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca
     6061 accagaccag cggatattcc agccctttcg atttatggat cgtgacaatc tgcaccagat
     6121 gtttatcact ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat
     6181 gttgcgataa ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgaa
     6241 gcagttcgct gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca
     6301 agttttctgc aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt
     6361 tgcgccagat ttgccgataa ccatcgaact cttcgactac cgcatcccac gcatgttcgt
     6421 cattattcag cgtttcaata tccagcgcat tcagccccat cattgacgtt gccagcgcac
     6481 tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct
     6541 gcgcttccag agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca
     6601 gcaacgttaa ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacgctga
     6661 tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct
     6721 gtccggcttg tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat
     6781 agtcgccaac gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt
     6841 cacctttaaa tacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg
     6901 gtatttcgcg aaacatgaac gtgtcatcag tctggctgaa aagcttgttc acactgttca
     6961 ccattccagg tgcggagcgc cagttggtgt ctaaagtgta gtgggcatga acttcactac
     7021 gcgccttcat ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt
     7081 cgccaatcag caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt
     7141 actgctgggg gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga
     7201 tcgccgctgc caacacctcg ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca
     7261 tgtcatcaaa acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct
     7321 cagccaatgc gcgggtgatc aacagatcgc ggatcgacaa tggttctgca agcagttgat
     7381 cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg cgatcttcta
     7441 agaaacgctg ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt
     7501 ctgcccaggc gctgatcttt tcgatccatt tagcctgatt gctacggtta aacttgcgtc
     7561 gatcaatacc agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct
     7621 gttttaccgt atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat
     7681 cgggcggcgg tgctttgata accggcggtt cgccttgcag ataacgatta atatcgcgca
     7741 gcaacgcctg cggccctttc caggtttcaa agacgacctg ggctatttca cgcggcagcg
     7801 ggtagcagtg gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt
     7861 caatcagctg ctgctcaaac agcatgccgg attcaaaggc attcaggttg agcatgcgct
     7921 ggcaaaagcc gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca
     7981 accactgcgc ggcttgcgct ttatcgtcga tctcttccag caggcgttcg tacagtggat
     8041 tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac
     8101 cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacgatca
     8161 gcgggcgggg aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg
     8221 caatcgtaaa ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg
     8281 gcaagcgcaa aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc
     8341 attgtttgct gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat
     8401 tcggctttcc cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg
     8461 accaccgcct gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc
     8521 acgattttat cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc
     8581 gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg
     8641 atttgcgcaa actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac
     8701 gccttgtaac gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc
     8761 atgccccact gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct
     8821 tcggtacgca actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag
     8881 gctgagctgg tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg
     8941 gtgctgttac cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg
     9001 tttcgacacc actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt
     9061 gttgcctggg cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt
     9121 aaggcgtcgc gataggccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt
     9181 tcatctcgcg agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca
     9241 aacgctttac ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca
     9301 agctgctctt ccgtagcggt atagctaaag tacccctcga gcaatgcctg gaacagctgc
     9361 ggcagacgct gggtgtaacc attagcatta atcataaggc cgttgttagc gttggtggaa
     9421 aaacttatgc caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga
     9481 taatcattga gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc
     9541 aaaatcaggc tgacatcagc tttgggctcg ctggcaaaat aacggcttgg cgcatacacc
     9601 acgcgcagat tcgactcatc aacaatcagc tctggatggt cgtatttctt ctctgactta
     9661 atcagcgaga aatcatcagg aatataaggg ttaagctctg gcaaagagag cgcaatgtcg
     9721 gcggcttttt tctgccagtc ggcgaaagtt tgtgcgctga ttttatcgac ctgatacggc
     9781 gcatcgacaa agtaagccgt tttgttgtgc ggctctttcg ggctgatata ccagatacgc
     9841 gcattctgcg gcgtcatcat cgccagacgt tcctttactg ctttagcatc gtaccgatcg
     9901 gcaatattga ctgcatccag cgtatgctca acaggaacgc gaatcatggt atctgccagc
     9961 cattcgacgt aatccatatc acgggtgatc gacggataac ggaagtcgat atccagcaca
    10021 ttcgccagtt catcgaagta ttgtttatca atgccttttt cacgtaacag attgagatag
    10081 ctaaaaattg ccgccacaac ctgatcgcga ttagccaggc ctttatcagt taaagacgca
    10141 gagatcgcta atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc
    10201 tcaactaatc cctgcttttg cagccagtca gaaagtgtac ctgggctgcg attgccaatc
    10261 agataggtaa tcaattcatc ggttttacta cggaacttcg ctgagttgtt atcgatgcga
    10321 aactcaacgc gcaacacttt acgcggcagc gcagggacgt aatgaatgat aatgcccttt
    10381 tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg
    10441 cgaccaaagg tgtccgccgc catttttgcc aactccggca gcggtttatt actgtaaata
    10501 accgccttca tcaaattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc
    10561 accggattac caggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg
    10621 gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta
    10681 cgcgccatgg ttaattcagc gttcactgca ttacgctcac gttcggcgta tttcttgtcg
    10741 agcaagggtt ctgcaatagc atcggccagg cggtctaccg caccgggcaa ggcgtcgttc
    10801 tcaacttcca gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg
    10861 tgcattttga gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga
    10921 ctcatatgtt caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca
    10981 acgggcacta ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc
    11041 accataccgt tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg
    11101 atggtttcct gaatcggctg ccatcccgtt tctgcctgac ttaatggtgc ccagagggca
    11161 acaaacaaca ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa
    11221 ctttattttc tggacagatt caacaggtta acgcaaatca tctgccagaa tttaatcttg
    11281 tgctgcacga gccagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa
    11341 agtaagcaga tagattgcgc aatttttata caacactcat gactgattaa agcgaaacag
    11401 cggtaacagg aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg
    11461 ccgccagagc cgttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc
    11521 gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc
    11581 atcgttttgc gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag
    11641 caatggcgcg gacattcctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc
    11701 ctgttcggct gcaagcggcg gaaaacgcca ctcaccgtct ttgcgtagaa aaaggcgact
    11761 ttcgccatta ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg
    11821 cgccacactt aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa
    11881 ccagccagtt atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg
    11941 gcgacaggca atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa
    12001 aatttcacca aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg
    12061 ttcggcatca tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct
    12121 aagtccttcc agaataaatg gctcggtgtc tgggatttcg ctgtcttcag tatggaagtt
    12181 cacctgcaaa cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt
    12241 ttccagcggc acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc
    12301 tttaccagcc tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg
    12361 ctggtagttt tgcggatcaa acggcatccg ggtatggagg caagtaagat gcgcttttac
    12421 ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc
    12481 gatgtagtcg atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat
    12541 ggaacgcccg atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa
    12601 caggtagcgg tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag
    12661 gtcaaagccc aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca
    12721 aaccactttg aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc
    12781 gagaaaacgc tggctgatac gttcttgatc cagacgctgt gccagttcat cacgcaatag
    12841 tgacagcggc accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca
    12901 ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt
    12961 gagcatatcg cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg
    13021 acgccagatg tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa
    13081 gccgctcgat tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc
    13141 gtagcccagc aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag
    13201 ttccagctcg cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca
    13261 ctggcgtaaa taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac
    13321 atccagcagc gccagcacat cctctgacac aaagcgactg tcaggcagtg ataacaggct
    13381 gataaacgct tccagtaccg gatgtgactg ccgcgcacga cggtcggaaa tggcgtaagg
    13441 caggtaacga tccgcaggtg cgctaccaaa cactgcctga ataaacggac tgtagctgtc
    13501 gatatcagcc accatcacga tgatgtcgcg cggagtaagt gtcggggctt cctccagcat
    13561 cgccagcaag cgatcgtgta aaacttcaac ttcacgctgc gggctatggc aaacgtggaa
    13621 tgtgatactg ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc
    13681 gatgttcaca cccgcaacgg cgcggttttc cagttccaga atgtcagact gaatgttatg
    13741 cagcaggtta tctggcgtca catcgacaaa agcgtccagc tcctggctgc tctccaggtc
    13801 agaaaggaga taaatgtagt cgcgcccaag cttcccccat gaggccagca gcgagttgcc
    13861 gacatcctgt tcaccatcgc tgttaaagag ctgtccggca ttttcgctat cgcgaaatag
    13921 cggcaattcg cgatcttcaa aactgtgtcg acgctgacga gtcagcagtt tcgccagata
    13981 agcaggatct ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat
    14041 ttcaatatgt ttacccagcg cctggagcgc ctggagataa acaggcggta acgcggaaat
    14101 accgcatata aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt
    14161 ttcgataaag cgctgataga gattcgcgcg gtgccagcgc ggttgtccga gttcatgggt
    14221 atattccacc agcgccttcc acaacggggc ttgccaggcc tgtgcttctc ccagcccttc
    14281 aaccaaatgt cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc
    14341 aaacaggtcc gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagt
    14401 cagataatgc cgcaacaggg taaagtcttc gcgctccagc aattgcggca gcagagtcat
    14461 cagtttccag ctcatgctct gtttgttaaa ggcgctctct ttggggattt ccggtaacac
    14521 ccggacgaac atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat
    14581 accaaacttt tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag
    14641 aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa
    14701 cgcttccagc acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta
    14761 ctgacaattc gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag
    14821 cgtaacgctg atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt
    14881 ggcaggtggc gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagcttttg
    14941 gtactggttt ctgctggcga gactgttcat tgatgttcgc tggatacccg ataacgcagt
    15001 gacaatcatc accatcagca ccatcagcac catcagcacc atcagcacca tcgcaaccat
    15061 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa tgccccctct
    15121 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatttttcc atcaatgact
    15181 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    15241 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    15301 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    15361 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    15421 attccttgca atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    15481 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    15541 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    15601 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    15661 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    15721 tcagacgaaa tccaatctgg tcggactctt ttaccggttc gcgatcccag ataccgttac
    15781 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    15841 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    15901 gcagccagat ttcatcttcc agtgccagct tacgagtgct cgttaaactt tcacgctgta
    15961 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    16021 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    16081 ataaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    16141 gaagcgaata tgcacggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    16201 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    16261 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    16321 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    16381 ccgcgcctag ctggcggttt gccaaagccg ttgcgactgc tgccagtatt gccagccata
    16441 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    16501 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    16561 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    16621 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaccgacgc acactggcgt
    16681 cggctctggc aggatgtttc gtaattagat agccaccggc gctttaacgc ccggatgtgg
    16741 atcgtagcct tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg
    16801 tttgataatc aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc
    16861 catatggttg ctgtacaggt gcgtatcgcc accggtccag acaaaatcac ccacttccag
    16921 atcgcactgc tgcgccatca tatgcaccag taacgcgtag ctggcaatgt tgaacggcag
    16981 gccgaggaag acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac
    17041 atagaactgg aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt
    17101 ccacgctgaa acaataatgc ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt
    17161 agtaatctgg tcaatatgac gaccatctgg cgttggccag gcgcgccact gtttaccata
    17221 cactggcccg aggtcgccat tttcatcggc ccattcgtcc cagatggtga cattgttttc
    17281 gtgtagataa gcaatgttag tatcgccctg caggaaccac agcagttcgt ggatgatgga
    17341 acgcaggtgg caacgtttgg ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat
    17401 ctgatgacca aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc
    17461 gtcgagcact ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg
    17521 ctgtgggctg cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat
    17581 ggaaagaatt tgccccatgc tgatgtactg cacccaggca ccggtaaact gcgcgtcggg
    17641 ctggcggaaa aactcaacaa tgatgcgaaa cgcgccgtag ccaatcagga acaagcctga
    17701 gacagctccc attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac
    17761 accttccagc agcagttcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc
    17821 gaaaatggat tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc
    17881 tgggaacagc atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat
    17941 aaagttgccc agacgcccgg caccaagacc aaacggaatg agtggtgcga taaaatcaga
    18001 gacctggaag aaggaacgtt tagtacggcg ggcgaagata atcatcacca cgataacgcc
    18061 aatcaggccg ccgtggaaag acatgccgcc gtcccagaca cggaacagat acagcggatc
    18121 ggccataaac tgcgggaaat tgtagaacag aacataacca atacgtcccc cgaggaagac
    18181 gccgaggaag cccgcataga gtaagttttc aacttcattt ttggtccagc cactgcccgg
    18241 acgattcgcc cgacgtgttg ccagccacat tgcaaaaatg aaccccacca gatacatcag
    18301 gccgtaccag tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa
    18361 atgcagatag ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac
    18421 agcgattgaa atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag
    18481 gggacctggc tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc
    18541 tccgcgaatc agcccgccca tgccgcgacg ctccataaag gctgcaacct gatgacgaac
    18601 ttcggtcgcc agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cggcaaaatc
    18661 aatgcgccgc agcaggtatt ttacccgcgc tacagaacgt ccgttcatag acagatggcg
    18721 atacccaagc ccaatgagga ttgccacgca catgggatcg cccgccattt caccgcacaa
    18781 acgaagatcg ataccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat
    18841 tgcaggatga agactgtcgt aaatgttcgc cacccgggta ttgttgcgat caacggccag
    18901 aatgtattga gtcagatcgt tggtgccaac agagatgaaa tcgaccctct ttgccagatg
    18961 cggcagcata aataccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat
    19021 ttcgtaaccg atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc
    19081 gtcaacttca tcgaggctgg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc
    19141 attagcacgc agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat
    19201 gcgaatccca cgccaaccca ggcatggatt ctcttcgctg atcggcatgt aaggcagctg
    19261 cttatctgct ccgacatcca gcgtacgcaa ggtgacgggt ttatcattga acatttgcag
    19321 catcccctga tactgcgcca cctgttcttc ttccgacggg aaaccacttt gcagcatgaa
    19381 tgggatttca gtgcgataaa gaccgatgcc atcaatacag ctgcccagtt tttcttcatg
    19441 ttccgggctt aaaccagcat tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc
    19501 gggtaaattg acgtcatctt ccgccagacg gctaagctca atctcttcac taattagccg
    19561 ctgatattct tgcagcagta ccggctccgg atcgaccagc aattcaccgc gatagccatc
    19621 aacgatcagc gtcctacgat gcagcaccga aggctgaata tccgcgccca tcacggtagg
    19681 gatccccagc gcacgtacca tgatcgcagc atgggagttg gctgcgccat ctcgcacgac
    19741 aacaccgact aagcgatcct ggggcagctc agcaagagtt gtcgctgaca gttcatctgc
    19801 caccagaatg aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa
    19861 cagcaatcgc tgacccagcg cacgtaaatc accagcccgc tctttgagat agttatcgct
    19921 tagcgcggca aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac
    19981 cgagccttta tcaacctcgg caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg
    20041 cgagtaaaga tcgaaaatag ccgccgtttc tttttgtgct ccggcggcaa agcgtttgct
    20101 gtagcggcga aactcgtttg ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag
    20161 agccggatcc agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg
    20221 ccagccttcg gcaatcgcca caccaggtgc tgccggtaat gcgcggattc gcgtctggcg
    20281 atattgccca aacagggcag tcaactgcga ctgagaaaga atagctgcca tctgggtggc
    20341 aagcgtcacc aggaaggatt cttcactttc gtcatactgg cgcaactctc gttgctgtac
    20401 caccagtaca ccaagcaact ggcgacgttg aataattggt acgcctaaaa acgcgcggaa
    20461 acgttcttct tttacggagg ggatgtattt gaagctgggg tgcttttgcg catctgcaag
    20521 gtttatcggt tccgccagcc tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt
    20581 tacagtgcga ccgcgtggtt ttttcagccc ccgggtcgcc atcaggtagt aacaacgtcg
    20641 atcatgatcg gccaggtaga ccgaacagac ctcggtatcc atcgcaagac agatgtcggt
    20701 aaccagaata tttaacgcct cattcaggcg tggtgcgctg gctacctttt cgactatttc
    20761 gcgcaggcga gtgagcataa ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc
    20821 gttttgtggt tttggcgtat tttcctgcag tgacatcacc acactcgcga actctttcat
    20881 taccctacgg tagacatcac gtttaaatga caccacctgt ctgaccggat accagtaact
    20941 tacccatcgc cagccgtcaa actctggtgt actgctggtt tgcatattga tttctgcatc
    21001 gccgcttacc agctgcaaga gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt
    21061 gtcccaacgc accaaacgtt tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag
    21121 gattcgaacg tctttgcggc ttaatcctac ttcttcaaac aattcacggt acatcgcctg
    21181 ctctgcggat tctccggggt tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa
    21241 tcgccgggcc cacattacct gcccctggcg attacaaatc acgataccta cgtttgggcg
    21301 gtagccatcg tcatcaatca ccggactacc tcaaaataaa gctttatata cgaatgattg
    21361 tttcatactc caggaagacg gtaaaccact ctctgcaggg cattacacac taataacaat
    21421 tgaataactc acagttatgt gcagagttat aaacagagga aggggtggat agccgttttt
    21481 attcaccttt tctgtggata gagttgtgaa gaactacgga attactacgg gaaaacccgg
    21541 agcattctga ataagcccga tatagacatt ttaaaatatt cattattagt caatgcatta
    21601 catgtaattt gagtaatgaa aaagagtaat tcgtgaccca ggtcacacct ctcatttacg
    21661 ggttggcgaa agatcaacca atgccgtgtt tatccacaga atgtgccact aagttaagca
    21721 ctgaaccact aaaaactgga gtttcgtcgc acgtcaaggc tgtaaatgaa aacagtagtg
    21781 gaggtttttc acagttatcc cagctttctg tggataacat ggtgtaagat cctgtttatt
    21841 ttcagtgacc agatttggaa aacctattgc agtgttgcgc aactcgatta ccggcagcct
    21901 aaaaagcaat ataaatcaga gaattgaaca acacatgtgg aaaaagttac actgcgaata
    21961 ttcggcacat aattgctgtt tgttttttaa ccaaggtatc atgacatgtc ccaacctcgc
    22021 ccactgctct ctcctcccga aactgaagaa cagttgttag cgcaagcaca gcaactttct
    22081 ggttatacat tgggagaact ggcggcactt gacgggctgg ttacgccaga gaatttaaaa
    22141 cgcgataagg gctggattgg cgtgttactg gagatctggc taggtgccag cgcagggagt
    22201 aaacctgagc aagattttgc tgctctgggc gtggaactta aaactatccc tgtggatagt
    22261 cttggtcgtc cgctggaaac aacattcgtt tgtgttgccc ggttaacggg taatagcggg
    22321 gtgacctggg aaaccagcca tgtgcgccac aagctcaaac gcgtactgtg gataccggtt
    22381 gaaggcgagc gcagcatccc gctggcgcag cgtcgcgtag gatcaccgtt gctgtggagc
    22441 ccgaatgaag aggaagaccg acagctacgc gaagactggg aagaattaat ggatatgatt
    22501 gttctcggtc aggttgagcg gatcaccgct cgtcacgggg aatatttaca gataagaccg
    22561 aaagcagcga atgcgaaagc gcttaccgaa gccattggtg cccggggcga acggattctg
    22621 acgctgccac gcggctttta tttgaagaag aatttcacca gtgcactact ggcccgtcat
    22681 tttctgatcc agtagccatc gctttgacct gccgctttcc gggcatataa ttaccgcttc
    22741 atttttttgg cagggctttt tagatgttat ttgcatggat aaccgatcct aacgcttggc
    22801 ttgcgctcgg tacgctgacg ctgctggaga tcgttcttgg gatcgacaat attattttcc
    22861 tttctctggt ggtggcaaag cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt
    22921 tggcgggagc catggttatg cgtctggcgc tgctggcatc aatctcttgg gttacgcgcc
    22981 tgacaaatcc gctttttaca atattcagtc aggaaatttc tgcccgtgat ttgattctgc
    23041 ttctgggtgg cttgttcctt atctggaaag ccagcaagga aatccacgaa tccattgaag
    23101 gtgaagaaga agggctgaaa acacgcgttt catcattcct cggcgctatc gtgcagatta
    23161 tgctgctgga tattatcttc agcctcgact cggtgattac cgctgtgggt ctgtcagatc
    23221 acctgtttat tatgatggca gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc
    23281 gctcgattgg tgattttgtc gaacgccatc cttcggtaaa aatgctggcg ctctctttcc
    23341 tgattctggt gggctttacc ctgattctgg aaagtttcga catccacgta ccgaaaggtt
    23401 acatctactt cgcgatgttc ttctctattg cggttgaaag cctcaacttg attcgcaaca
    23461 aaaagaatcc gctctgatac tccgtacgct ctcctgcggg agggcgtact cactcctttt
    23521 accctcacag tttaagattt tcctgctttc aagatatata acgtcggttt ataaacagac
    23581 tattatcata ggtaggccaa atgaggataa ccggatgaaa aaatgggcag taataatttc
    23641 tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt gattacgtca tggcgaccaa
    23701 agatggccgt atgattttga ccgatggaaa acctgaaatt gatgatgata ccgggctggt
    23761 gagttatcac gatcagcaag gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat
    23821 tattgaacgt taacaaataa ggtcagcatc cggctggcct taagattttt ctctttccct
    23881 tttcccttcc ctctgccatt tttatattcc ttatgtcgtg attataaaaa ggaaacggct
    23941 atgcaatatc accgtatacc ccacagttcg ctggaagtca gcacgctggg gcttggcacg
    24001 atgacgtttg gtgaacagaa cagcgaagcc gacgcccacg cacaactcga ctatgccgtc
    24061 gctcagggca ttaaccttat cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa
    24121 acgcaagggt taaccgaaac ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa
    24181 aagttaatta tcgcctccaa agtgagcgga ccgtcgcgca ataatgacaa gggcatccgc
    24241 ccggatcagg cgctggatcg gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgc
    24301 ctacagactg attacctcga tctttatcag gtgcactggc cgcagcgccc gaccaactgc
    24361 ttcggcaaac tcggttatag ctggacggat tctgcgcctg cggtttcgct gctggatacg
    24421 ctggacgcac tggcagagta ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac
    24481 gaaactgcat ttggcgtaat gcgctacctg catctggcgg acaaacacga tctgccgcgt
    24541 attgtcacca ttcagaaccc ttacagtctg ttaaaccgca gttttgaagt aggtctggca
    24601 gaagtcagcc agtatgaagg ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg
    24661 ctgaccggga aatatctcaa tggtgcaaaa cccgctggcg cacgtaatac gctctttagt
    24721 cggttcaccc gctatagcgg tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc
    24781 gccagacgtc atggccttga tcctgctcag atggcgctcg cgtttgtacg ccgtcaaccg
    24841 tttgttgcca gcactctgct gggcgcaacc acgatggatc agctgaaaac taacatcgaa
    24901 agtttgcatc tggagttaag cgaagacgta ttagctgaaa ttgaagcggt acatcaggtt
    24961 tatacttatc ctgcaccata aagattaacg ccggttaaaa ccggcgttaa atattaatga
    25021 cggcgctgcc agatccacag cgccgttatt gccagcgcaa atagcgcacc gaagccaatg
    25081 ccaatgggca cgaccgggat gcctaccatt accgccagcg agtaaatgcc cagcatcaac
    25141 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    25201 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccagcacg
    25261 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga gaaaatcggt
    25321 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaacc
    25381 agcttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    25441 gtcggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    25501 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    25561 ccattgcacc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    25621 caggactgcc ccggacgcgc cgccagtttg ggaatgtaga tattggcaac gaccgcacca
    25681 ccgtaggcca gtgcgcatgc ggccagagcg accaggacat gccagtcagc cagcacacca
    25741 ccggctacgg aaccgagtaa aatcgccgct atggcagaag cttccattaa accgttagct
    25801 tttactaact tactacccgt ggttaattcg ccgagaatac cgtatttcgc cggtgaatag
    25861 gctgcagcac caacacccac cagcgtatag ccgagaaacg gattgatacc aaagcagata
    25921 ctggctgcgc ccagcagctt caggccgttg gcaaacatca tcacccggcc tttggcgaag
    25981 ctatccgcca cctgcccgac aaacggcgcc aaaagaatgt aagcacctac aaacaccatt
    26041 tgcaggatgg gctggctcca ctccggatag aactgcgctt tcagtaacgc cagagtggca
    26101 aacagtagcg cgttatcgcc aaacgcagag agaaactgcg ccacgataac cgctttcatc
    26161 cccttcgacc acaacgaagt gttagtgtgc actgactcac tcatcgtgtt gttccgcttc
    26221 gtctacccag cttttcaacg tgacaaagtc aggtttgccg ctgccaagta atggcatctg
    26281 tttcagatag cgaatatcgc gcggtacagc aagctccggc acgccgtgct cgcgggcata
    26341 ctgttgcaac ttatcgcgcg tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc
    26401 tttgctggca tcgctcttaa tcgcagtggc atggacttta tctggcgaaa caccaagtgc
    26461 cagttgttcc accatttcca gcgacaccat ttcgcctgca attttggcaa agcgttttgc
    26521 gcggccctga atctgcacaa agccctgctc gtcaaaacgc acaatatcgc cagtgtcata
    26581 ccagccgcgc tccatttcgc cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg
    26641 cttctccacc cgcagatagc cgttcattat gttcggccct ttcagttgca ggcgtccgcc
    26701 ctcttcgata ccagggaccg acaacaggcg tgcatccatt cctggtagaa tacgccctac
    26761 cgtaccgggt ttcgccgcca tcggtacgtt gatagaaacg acaggcgcgc attcggtcac
    26821 accgtagcct tcaaggatgc gcaggccaaa tttatcctgc caaagctgtt tggtactttc
    26881 ttgtagtttt tctgcgcctg ccaccacata gcgtagacga tagaagtcat acgggttggc
    26941 gaaacgcgcg tagtgaccga ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc
    27001 atacaccaag tccggcacaa tgcggtaatg cagcgggctt ggataaagga acacttctgc
    27061 acctgtaagc agtggcgtaa acaggcctac cgtcagccca aaggagtgaa acagcggtaa
    27121 tgccgacata aagcgatcgt tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt
    27181 cgccagaatg cttttatggc tatggacgac gccttttgga tggccttcag aaccggaggt
    27241 aaagaggatc agcgcctctt cttccggctg ctgttttacc tgcgccaggc gcggcatcag
    27301 caaatgagcg aagatccata ctttgtcggc agtggtgaca tctgctttta aatcttccag
    27361 atagacccag cgcacctgag taagttgctc cggcagatgc cagagtttgc ctttatcgag
    27421 aaactggcgg gaagtgaaga tggttttgat ttcagccgcc gtaatagcac tggtcagccc
    27481 ttttaccccg gcagtgtagt tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa
    27541 aatcactgcc gcactgatgc ctgcattggg cagcattaag ccgatgcgtt cgccttcaac
    27601 actgtatttt tcaaggatgc gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga
    27661 gtctggggta aagttgacgt cttcgacaca tttcttcccg gctccgaagc ggtacattgc
    27721 actcagtaaa gattcgtaca gagtttcacg cgggcgcacc gccatacgcg cttccatcat
    27781 tatttgatgc agcatttcgc cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg
    27841 catcgccacc tgcgttggag gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt
    27901 aaccagacct ttcaggcggc tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat
    27961 aaccgttgca ccagacttcg ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga
    28021 gcctgtagtg gtgatgcgtc cttcagggaa aatcaccact ggtcggccct gttccaccag
    28081 acgtaccaga tgtttaatag ccataggttg cgtcgggtcg agaggaacaa agtcgataaa
    28141 tgatttcagc caacgcatat accactgttg gcttattgag gtgtaaacgg caaacactgg
    28201 acgcacaggt aaaaacagtc caagcaaaat gccatcaata aaagagacgt gattaggcgt
    28261 aattagtacg cgctcgccct tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa
    28321 aacacggcac aaatttcgaa aaaagctaaa aagcattcca actccctttg ctctgattca
    28381 gtaaaagcga atggagggag attacacgag ataaagaacg cgagcgacag taaattaggt
    28441 gcgaaaaaaa acctgcgcat ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta
    28501 ccgaataatc tcaccaatca atacctctgg gatcttgatt gtggtctgca cgacgactct
    28561 tcgccagcga gaaaacgcaa aggaatgaag ggaaatgcaa cgaggtgtgt aaattgtcgg
    28621 ttactgttac agattgatga ccggcaaaaa aaaacctgcg catctgcgca ggctggtgta
    28681 attcatgtgc tcaacacgaa gttgacttca cctatcaata cctctgggat caccacttta
    28741 gcaacctgaa gccaaacgcc accagcggtc aatcgcaaca gcgtttcgca aagtgtaacc
    28801 aaaggtttgc aatctctttt tttgtcttac ttatttgcgt ttggctcaca ttcccacgat
    28861 gaaaacacgc caccccttga accaacgggc gttttccgta acactgaaac aatgtaagcg
    28921 tttacccact aaggtatttt catggcgacc ataaaggatg tagcccgact ggcaggcgtt
    28981 tcagtcgcca ccgtttcccg cgtcattaat aattcaccca aagccagcga agcttcccgg
    29041 ctggctgtgc atagtgcaat ggagtctctt agctatcacc cgaacgccaa cgcccgtgcg
    29101 ctggcgcagc agaccactga aacggtcggt ctgatcgttg gtgatgtttc cgatccgttt
    29161 ttcggtgcaa tggtgaaagc ggtcgaacag gtggcttatc acaccggtaa tttcttattg
    29221 attggcaacg gttaccacaa cgaacaaaaa gagcgtcagg ctattgagca actgatccgc
    29281 catcgctgtg ctgcgttggt cgtccatgcc aaaatgatcc cagatgccga tttagcctca
    29341 ttaatgaaac aaatgcccgg tatggtgctg atcaaccgta tcctgcctgg ctttgaaaac
    29401 cgttgtattg ctcttgacga tcgttacggt gcctggctgg caacgcgtca tttaattcag
    29461 caaggtcata cccgcattgg ttatctgtgc tctaaccact ctatttctga cgccgaagat
    29521 cgtctgcaag ggtattacga tgcccttgct gaaagtggta ttccggccaa tgatcggctg
    29581 gtgacatttg gcgaaccaga cgaaagcggc ggcgaacagg caatgaccga gcttttggga
    29641 cgaggaagaa atttcactgc ggtagcctgt tataacgatt caatggcggc gggcgcgatg
    29701 ggcgttctca atgataatgg tattgatgta ccgggtgaga tttcgttaat tggctttgat
    29761 gatgtgctgg tgtcacgcta tgtgcgtccg cgcctgacca ccgtgcgtta cccaatcgta
    29821 acgatggcga cccaggctgc cgaactggct ttggcactgg cggataatcg ccctctcccg
    29881 gaaattacta atgtatttag tccgacgctg gtacgtcgtc attcagtgtc aactccgtcg
    29941 ctggaggcaa gtcatcatgc aaccagcgac taaccgcagt taaagcaatt ccagcgccag
    30001 taattcttcg atggtctggc gacggcgaat caaccgcgcc tgaccattat caaacagaac
    30061 ttctggtaac agcggacggc tattgtagtt ggatgacatt gatgcgccat atgcccctgt
    30121 atcatgcagt accagataat cacctgcctt cacttccggc aaggcgcggg tttcaacatt
    30181 tcccccttcc tgctgggtaa agacatcgcc cgattcacat aacggcccgg cgacgacggt
    30241 ttccaccgtt ggcgcgtgtt ccagagaacg accatcagct gccagggcac tgatatggtg
    30301 gtaactaccg tacattgccg ggcgcatcag atcgttgaac ccggcatcaa ccagcacaaa
    30361 gtggcggcta cccatttgtt tgacgctccg cacctgagta attaatacgc cagactgcgc
    30421 taccaggaag cgacccggtt caatttccag tttcacaggg tggcccaaat ggcgagcgat
    30481 ttgctcacgc gcggcattcc acagaccata ataatgttcg gtatcaaccg cctcttcacc
    30541 ctgttgataa ggaatagaaa gcccaccgcc cgcagaaata gcctgtaaat cctgaccgaa
    30601 ttcgataacc tgacgcacca tagcaccaca cacctgctcc agatgggcat aatcaacgcc
    30661 agaaccaatg tgcatgtgaa tgccgactag ctgcagatga tgacgttgta tcacgtccag
    30721 tgcggcgggc agatcggtgt accagatacc gtgcttgctg ttttcgccac cggtattggt
    30781 tttttggcta tgtccgtgac caaaccccgg attaacgcgc agccataccc gatgccctgg
    30841 cgaaacctgg cccagttggt cgagcgtatc aacagaaccc gcattcaccg gaatttgcaa
    30901 ttcactgacg cgttcaagcg tcgcctgatc gataacatct gccgtaaaaa caatatcatc
    30961 ggggtgcgtt tgcggattgt aacccgccgc caacgcacgc tctatttcgc ctaacgagac
    31021 ggaatccact ttcacgccct gctcacgcat taagcgcaaa atatgaatat tggaacaggc
    31081 tttctgtgca aagcgcacca cttcaaactg tttcagcgct gcaatctgcc gacgaataat
    31141 ttgcgcatcg tagacccaca ccgggcagcc aaattcagcg ggcaaacgca gcagattttc
    31201 ggcggtgaga tcggtatcgg tgctgaacag tgaatgtggc ataacaaact ccagatgagt
    31261 gcttttttat gattacgcca catcataaaa agaataaaaa atatcgattt atgtcgagtc
    31321 tatgcaaaaa tgatatggat taccggattg cgagagagcg ctaatggccg ccgttaactt
    31381 acgtcatatt gaaatttttc atgcggtaat gaccgccgga agcctgactg aggcagcaca
    31441 cctgctacac acctcacagc caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat
    31501 cgggctgaaa ttgtttgagc gcgtacgtgg acgattacat cctaccgtgc aaggactgcg
    31561 tctgtttgaa gaagtgcaac gatcctggta cggactggat cgcattgtca gcgccgcaga
    31621 aagtctgcgc gagtttcgcc agggagaact gtctattgcc tgcctgccgg tcttttcgca
    31681 atctttttta ccgcagctcc tgcaaccctt tctggcacgt tatcccgatg tcagcttaaa
    31741 tatcgtgccc caggaatcac cgctacttga agagtggctc tcggcccagc gtcatgattt
    31801 aggactcact gagacactcc atacgcctac gggaacagaa cgtaccgaat tactctcttt
    31861 agatgaagtg tgtgtgttac ctccgggtca tccgctggcg gtaaaaaagg tattaacgcc
    31921 ggatgatttt cagggtgaga attacatcag cctttcccgt actgacagct atcgccagtt
    31981 gctggatcag ctatttactg aacatcaggt taaacgacgc atgatcgtag aaacccacag
    32041 cgccgcgtca gtctgcgcaa tggtacgggc gggggtaggt atttcggtgg ttaacccgct
    32101 caccgcacta gattatgcgg caagcggttt agtggtgcgg cggttcagta ttgcggttcc
    32161 gttcaccgtc agcctgatcc gccccctgca ccgcccgtca tcagcgctgg ttcaggcgtt
    32221 tagtgggcat ttacaagcgg ggttaccgaa actggtcact tctcttgacg ctattttgtc
    32281 gtcagctacg acagcataaa agcgacagca tcctcggcat ggatcgccgc ggtatcaaac
    32341 acaggcaaaa cactgcgctc ttctggcacc agtaaaccaa tttctgtgca gccaaaaatg
    32401 acgccctgtg cgccctgttc tgcaaggcga gcaatcactt gcgcataata agcgcgtgac
    32461 gcttcggtaa attgccccag acacagttct tcaaaaataa tctgattaat tttcgcccgt
    32521 tcatccgctt caggaataag acaattgatg gaaaattgtt ccgtcagccg cccgcgataa
    32581 aaatcctgtt ccatggtgta acgcgtaccc agcagcgcca cacgagtcat tccggccccg
    32641 gtaattgcac gtccggtggc atccgcaatg tgtaagaaag gcagagtgca acgtgactca
    32701 atggcatccg ccactttatg catcgtattg gtacataaca caataccttc tgcgcccgcc
    32761 cgctgtaagc caagcgccgc ctcagccaga atgtccccgg ttttatccca ttccccgcga
    32821 cgctggcact cttctatttc atgaaaatcg acgctatgta gcagcatttg cgcagagtga
    32881 agcccaccaa gccgctgttt aatgccttca tttatcagac gatagtaagg aatggtggat
    32941 tcccagctca ttcctcccag caaaccaatt gttttcattc ctctctcctg atgtgtgtta
    33001 ccgcaacaag gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct
    33061 attaacgaaa aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat
    33121 ttctcaactt ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg
    33181 tttccggaat gagccagaaa gtaataccca caaacgcaat gttcagcgca gtgtagagcc
    33241 agaacgtacc ggcagcgcca atgctatcaa gcagtgtcag gaaggccgcg ccgataatca
    33301 tattcgacac ccagtttgtc gtcgtcgaac aggtaatacc gaaatcgcgg catttcagcg
    33361 gctgaatttc agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa
    33421 tacacatcat cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat
    33481 caaactgcat caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa
    33541 ttttcagagc cggtttacga cctgctttat ctaccgtaaa gaccgcaatg aaggtcgcga
    33601 acataaaggt cagtcccacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc
    33661 ccgccatttt gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct
    33721 gcatcgcctg caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa
    33781 acagtgccca accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc
    33841 gcgctttttc cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc
    33901 ccttttccgc cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagaa
    33961 ctgctggtaa agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg
    34021 tatcggataa aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca
    34081 tcttaccgcg aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg
    34141 cgatcccgac agcaatgccc agcaccacac gagcggcgat taacatctct acgctggtcg
    34201 caaaagcgga ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt
    34261 tacgccccag gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca
    34321 tcatgctact aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga
    34381 acggcaacgc tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag
    34441 cagctaccga aacaaacata ttcatacgcc gcgtatcacg caaagaacgt ggcgttaaag
    34501 cagattccgt attgatagta accatttttt cctgccagca gagagtaaga cattgtgaaa
    34561 aaatacgtga acaattcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt
    34621 gctgcgacat gccgttatgt gatggatatt ccaattttaa aattaagttg aattattgag
    34681 attattatta acctcctaat tttaaagcag ataaaattca taaagttcat taattgataa
    34741 ttgatatgga ttatttcata accatgatat ggattatgat gatctagaga tataaaaaac
    34801 cctgccatgc ggcagggtca taaaagtaag aggaatgaat taacgcgcca gccaaccgcc
    34861 atccacagca atggtataac catttacata atctgaagcg ctggaggcaa gaaacactac
    34921 cggccccatc aggtcactcg gcagtcccca acggccagct ggaatgcgat cgagaatttc
    34981 cgcgctacgt tgttcatctg cccgcagttg ttgggtattg ttggtcgcca tgtaacccgg
    35041 agctatcgca ttaacattaa tgttgtgttt agcccattcg tttgccatca atcgcgtcac
    35101 acccatcacg ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga
    35161 gagcattgac gcgatattga taatcttgcc gccattgcct tgcgcgataa agtgtttcgc
    35221 cgctgcctga gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc
    35281 tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat ccggcgttat tcaccaggat
    35341 atcaatatga ccaaattcag ctaccgcgcg atccagcagt ccaggaatgc catcaatctt
    35401 tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gctcgatggt
    35461 ttcagtcggt tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa
    35521 cgccatcccc tggcccagcc cagtatcaca accagtgacg accgcaactt taccttggag
    35581 agaaaatgca cttaaaatca taacaacacc ttattcgttt atgcccacaa ctagcgcaaa
    35641 tctttaacgc ccacatggtc catatcatta aagacctggt tttcaccgac catgccccag
    35701 ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctcgg ggagatcacc
    35761 gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat catgtggaaa
    35821 acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg ctcgtgggtg
    35881 tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc catactcaat
    35941 tgacaggttt ccagtacatc cgggacaaaa tatttgttaa tcgtgcgacg gttactggtg
    36001 aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt ggtgggatac
    36061 gtcgtatgcg cgggtgcgca attgtaataa aacttcgccg gagtggcggt atcaatactg
    36121 gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg accgatttca
    36181 tagcattgac catcgacagt aatcgtaccg gcaccgccaa tattgataac acctaactcg
    36241 cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc aacggaaacc
    36301 gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct gtaaaccatg
    36361 gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag cccttgggta
    36421 tccagcgttt ttgcgtgcgc accgtggatg ctctgtctta cgtccacatc aacctccgat
    36481 aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt tctctataaa
    36541 gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt tttattttta
    36601 atttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg
    36661 agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg
    36721 gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa tggtcaatgc
    36781 cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat tacgtttcac
    36841 catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag
    36901 tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac
    36961 cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat cagccaactg
    37021 ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc
    37081 cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta aattcaacgc
    37141 tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta tggatgatgc
    37201 attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg ctaaaccttc
    37261 tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct gaccgttact
    37321 ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc
    37381 tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc catattcgcg
    37441 agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc acaacccatc
    37501 atgcaccaga ctgtcaacca actggctatt gccaagctgt tcaccggtgc ggctatcagt
    37561 cagaacatgt ggtgcgcggc tcatgttttc ctggccacct gcgatgacaa tatcagcctc
    37621 gccacactgt atcgcctgag tagccagatg cagtgcttta agaccggaac cgcaaacgtc
    37681 attaatcgtg attgcagaaa cgctgttagg aagaccacct ttaatagccg attgccttgc
    37741 cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat ccaccgcata
    37801 tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa gttccacggc
    37861 ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg ccccgacaat
    37921 cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa tgattgttaa
    37981 ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa tatgcctgag
    38041 cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac caatagatgg
    38101 gtagataggg cgttaatctc ccatacttaa cttggtttat ggtaaattgc cctccatttt
    38161 gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa gaagaaactc
    38221 tgtgaagttt cgcgctctat ggcacagccg ttgatgcagg cacgctattc cttcctattc
    38281 agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc tggcctttaa
    38341 catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca tcagcaggtg
    38401 aagggataac gggcgcggta acacactact atggcaagaa gattggtaat ctgattacca
    38461 cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca attaccaact
    38521 cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt atgttggtga
    38581 gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat gccactattc
    38641 gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt tccatttacc
    38701 ttgtcggtag ttggcaacct gatctattaa caacccaggt agagttcaat cagaataccc
    38761 ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat acgcccatta
    38821 tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg gataaatgca
    38881 aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc tttgtcttta
    38941 gctgcctgct ttctattcca tcttcgtata ttgaagcggc taaagaagaa ggggtcacca
    39001 ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt tccgggatta
    39061 ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc gttattgaag
    39121 gtgccacaga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa agtcgtgcat
    39181 ttaaccacgc actatcaatt atgttggtat cgctgattac cttcattgtt tgttgcatta
    39241 acccgaacgc gatttcgatg atttacgcga tcagcggccc gctcattgcc atgatacttt
    39301 tcatcatgcc tacgctgtca acgtatctca tcccggcgct taaaccctgg cgttccatcg
    39361 gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat
    39421 atccgttgtg gatggttcaa aattgctaca attttgaacc atcccatttt tcataatatt
    39481 catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta tcagttagtt
    39541 aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat aacttagatt
    39601 cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct cataccatga
    39661 gatagttatc tggaccacgt tcgaccatgc aagattaaat cagtgaaaca taatcatatt
    39721 taataacgag aaatgcattt ttaaatatct taatcagaat tatagcaaat acagattaat
    39781 cgcaattttt cccagcaaaa ttacaaaatc aaggtgtatt gacatcatca atcaccaatg
    39841 aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt agtcgttata
    39901 aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa gagtcaccgc
    39961 gtttgcagta ctgcgcaaac tcgtgttttg atcttaccca gcaatagtgg acacgcggct
    40021 aagtgagtaa actctcagtc agaggtgact cacatgacaa aaacagtatc aaccagtaaa
    40081 aaaccccgta aacagcattc gcctgaattt cgcagtgaag ccctgaagct tgctgaacgc
    40141 atcggtgtta ctgccgcagc ccgtgaactc agcctgtatg aatcacagct ctacaactgg
    40201 cgcagtaaac agcaaaatca gcagacgtct tctgaacgtg aactggagat gtctaccgag
    40261 attgcacgtc tcaaacgcca gctggcagaa cgggatgaag agctggctat cctccaaaag
    40321 gccgcgacat acttcgcgaa gcgcctgaaa tgaagtatgt ctttattgaa aaacatcagg
    40381 ctgagttcag catcaaagca atgtgccgcg tgctccgggt ggcccgcagc ggctggtata
    40441 cgtggtgtca gcggcggaca aggataagca cgcgtcagca gttccgccaa cactgcgaca
    40501 gcgttgtcct cgcggctttt acccggtcaa aacagcgtta cggtgcccca cgcctgacgg
    40561 atgaactgcg tgctcagggt taccccttta acgtaaaaac cgtggcggca agcctgcgcc
    40621 gtcagggact gagggcaaag gcctcccgga agttcagccc ggtcagctac cg
//
LOCUS       DAJTYH010000024        40261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTYH010000024 DAJTYH010000000
VERSION     DAJTYH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..683)
                     /locus_tag="OO609_001278"
     CDS             complement(<1..683)
                     /locus_tag="OO609_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6632766.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSS"
     gene            929..1555
                     /gene="gstB"
                     /locus_tag="OO609_001279"
     CDS             929..1555
                     /gene="gstB"
                     /locus_tag="OO609_001279"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6632767.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1552..2666)
                     /gene="yliI"
                     /locus_tag="OO609_001280"
                     /pseudo
     CDS             complement(1552..2666)
                     /gene="yliI"
                     /locus_tag="OO609_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2777..3160)
                     /gene="bssR"
                     /locus_tag="OO609_001281"
     CDS             complement(2777..3160)
                     /gene="bssR"
                     /locus_tag="OO609_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6632768.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3373..4698
                     /gene="rimO"
                     /locus_tag="OO609_001282"
     CDS             3373..4698
                     /gene="rimO"
                     /locus_tag="OO609_001282"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6632769.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4745..6073)
                     /locus_tag="OO609_001283"
     CDS             complement(4745..6073)
                     /locus_tag="OO609_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6632770.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6081..8423)
                     /locus_tag="OO609_001284"
                     /pseudo
     CDS             complement(6081..8423)
                     /locus_tag="OO609_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8601..9512)
                     /gene="gsiD"
                     /locus_tag="OO609_001285"
     CDS             complement(8601..9512)
                     /gene="gsiD"
                     /locus_tag="OO609_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6632771.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9515..10435)
                     /gene="gsiC"
                     /locus_tag="OO609_001286"
     CDS             complement(9515..10435)
                     /gene="gsiC"
                     /locus_tag="OO609_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6632772.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10453..11991)
                     /gene="gsiB"
                     /locus_tag="OO609_001287"
     CDS             complement(10453..11991)
                     /gene="gsiB"
                     /locus_tag="OO609_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6632773.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12011..13882)
                     /gene="gsiA"
                     /locus_tag="OO609_001288"
     CDS             complement(12011..13882)
                     /gene="gsiA"
                     /locus_tag="OO609_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6632774.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13869..14834)
                     /gene="iaaA"
                     /locus_tag="OO609_001289"
     CDS             complement(13869..14834)
                     /gene="iaaA"
                     /locus_tag="OO609_001289"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6632775.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15038..16273
                     /gene="moeA"
                     /locus_tag="OO609_001290"
     CDS             15038..16273
                     /gene="moeA"
                     /locus_tag="OO609_001290"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6632776.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16273..17022
                     /gene="moeB"
                     /locus_tag="OO609_001291"
     CDS             16273..17022
                     /gene="moeB"
                     /locus_tag="OO609_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6632777.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17201..17863)
                     /gene="fsa"
                     /locus_tag="OO609_001292"
     CDS             complement(17201..17863)
                     /gene="fsa"
                     /locus_tag="OO609_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6632778.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            17994..18893
                     /locus_tag="OO609_001293"
     CDS             17994..18893
                     /locus_tag="OO609_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6632779.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            18899..21331
                     /locus_tag="OO609_001294"
     CDS             18899..21331
                     /locus_tag="OO609_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6632780.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21477..22292
                     /gene="ybiV"
                     /locus_tag="OO609_001295"
     CDS             21477..22292
                     /gene="ybiV"
                     /locus_tag="OO609_001295"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6632781.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22444..23709
                     /locus_tag="OO609_001296"
     CDS             22444..23709
                     /locus_tag="OO609_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6632782.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(23950..25542)
                     /locus_tag="OO609_001297"
     CDS             complement(23950..25542)
                     /locus_tag="OO609_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6632783.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25761..26681
                     /gene="ldtB"
                     /locus_tag="OO609_001298"
     CDS             25761..26681
                     /gene="ldtB"
                     /locus_tag="OO609_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6632784.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26739..27857)
                     /locus_tag="OO609_001299"
     CDS             complement(26739..27857)
                     /locus_tag="OO609_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6632785.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27854..28321)
                     /gene="mntR"
                     /locus_tag="OO609_001300"
     CDS             complement(27854..28321)
                     /gene="mntR"
                     /locus_tag="OO609_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6632786.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28507..28635
                     /gene="mntS"
                     /locus_tag="OO609_001301"
     CDS             28507..28635
                     /gene="mntS"
                     /locus_tag="OO609_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6632787.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            28907..30490
                     /gene="opgE"
                     /locus_tag="OO609_001302"
     CDS             28907..30490
                     /gene="opgE"
                     /locus_tag="OO609_001302"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6632788.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30538..31053)
                     /gene="ompX"
                     /locus_tag="OO609_001303"
     CDS             complement(30538..31053)
                     /gene="ompX"
                     /locus_tag="OO609_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6632789.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31106..31171)
                     /locus_tag="OO609_001304"
     CDS             complement(31106..31171)
                     /locus_tag="OO609_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632790.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31406..32293
                     /gene="rhtA"
                     /locus_tag="OO609_001305"
     CDS             31406..32293
                     /gene="rhtA"
                     /locus_tag="OO609_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6632791.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32592..33095
                     /gene="dps"
                     /locus_tag="OO609_001306"
                     /gene_synonym="pexB"
     CDS             32592..33095
                     /gene="dps"
                     /locus_tag="OO609_001306"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6632792.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33499..34245
                     /gene="glnH"
                     /locus_tag="OO609_001307"
     CDS             33499..34245
                     /gene="glnH"
                     /locus_tag="OO609_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6632793.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34384..35043
                     /gene="glnP"
                     /locus_tag="OO609_001308"
     CDS             34384..35043
                     /gene="glnP"
                     /locus_tag="OO609_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6632794.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35040..35762
                     /gene="glnQ"
                     /locus_tag="OO609_001309"
     CDS             35040..35762
                     /gene="glnQ"
                     /locus_tag="OO609_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6632795.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36023..38247
                     /gene="ybiO"
                     /locus_tag="OO609_001310"
                     /pseudo
     CDS             36023..38247
                     /gene="ybiO"
                     /locus_tag="OO609_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38244..39170)
                     /gene="rlmF"
                     /locus_tag="OO609_001311"
     CDS             complement(38244..39170)
                     /gene="rlmF"
                     /locus_tag="OO609_001311"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6632796.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39446..39706
                     /locus_tag="OO609_001312"
     CDS             39446..39706
                     /locus_tag="OO609_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6632797.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            39971..>40261
                     /locus_tag="OO609_001313"
     CDS             39971..>40261
                     /locus_tag="OO609_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632798.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKN"
BASE COUNT        10023 a        10632 c         9806 g         9800 t
ORIGIN      
        1 ttgctgctcc acagcagacg gttacggtta ggctgacgaa ttttgttgaa ggtgaactct
       61 ttctctttat gaatggcgta ctcttccggc acatcgtgga tcaatgcttt acccagcaat
      121 gccatatcgc gcgcggtact gaactgcccc ggcgcatcca ggccgtgcac cgtctggaag
      181 gtagtgttgg tcagccccag ttttttggca taaccattca tcagaccaat aaaggactcc
      241 tggctcccgg caacgtaatc tgccagcgca atacaggcgt cattaccgga ctggataatc
      301 acacctttgt tcaagtctgc caccgaaacc tgatcgcccg gtttgaggaa cattaccgat
      361 gaaccacgca gtgccggatt tcccgtcgcc caggcatctt taccgaccgt caccatatcg
      421 gtgagtttaa tcttatcggc cttaagcgcc tgcccaacca catagctggt catgatttta
      481 gtcaggctcg caggatccag tttctcatcc gcattgcctt ctgccagcac tttaccgctg
      541 gcgtaatcca ttaaaatcca tgcacgcgca tccacgctcg gcgcttcaac ggtttgttcg
      601 gccgcgaatg ccgttggggc aaaaaggaat aaaaatgcag aacccgctgc aagaccacga
      661 aggagagagg agtattgcgt cataaatgcc acccgagtat ccattcaaaa aaatacgtta
      721 tatcgccgta tgataagttc ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa
      781 gttggtaaag tttttaaagt ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt
      841 tttttgccag ctgttgctta aaagttaact ttacttcacc atcggagtcc tgttcagtct
      901 gtggctgcct gtcggaaaag gggtgagtat gattacgctg tggggtcgga ataattcaac
      961 taacgtaaaa aaagtattgc tgacgctcga agaactggaa ctaccttatg agcaaattct
     1021 cgcgggccgt gagtttggga taaatcacga tgctgatttt ctggcgatga accctaacgg
     1081 gctggtgccg ttgttgcgtg acgacgaaag tgatctcatt ctttgggaat caaacgccat
     1141 tgtccgctat ctggcggcac agtacgggca aaaacgcctg tggatcgact caccggcacg
     1201 tcgtgcggaa gcggaaaaat ggatggactg ggcaaaccag acgctcagta atgctcatcg
     1261 cgggatcctg atgggattag tcagaacacc accggaagag cgcgatcagg ccgccattga
     1321 tgccagttgc aaagagtgcg acgccctgtt tgccctgctc gatgcggaac tggcaaaagt
     1381 aaaatggttc tctggcgacg agtttggtgt gggcgatatc gctatcgcac cgttcatcta
     1441 caatttgttt aacgttggcc tgacctggac accgcgtcca aatctgcaac gctggtatca
     1501 gcaactcact gaacgccccg cagtgcgcaa agtggtgatg attcccgtta gctaattgcg
     1561 tgggctaact ttaagtaatt ccccactgga ctcgtcggtg agaacgtata aataaccgtc
     1621 gggtccagtg cgaacatcac gaattcgctg ccctctgtcc gttaaaatac ggccatcttc
     1681 tgtcactttg tcgccgttga cgctcatcac aatgacatct ttatctttca gctcccaata
     1741 aataattttt gctgccactg ggggaattta tcgctgttat agaaggccat gccgctcaca
     1801 gcgggcgaat ctttccagta aaaaacaggt tgctcggtcc cggcgacgat ctcccctttc
     1861 gcttccggta tcttaaagcc tgaatagttg attccccagg ttgccagcgg ccagccgtag
     1921 tttttgcctt tttgcgggat attaatttca tcaccaccgc gcgggccatg ttcattcagc
     1981 cacagtgcat tactccacgg attcatcgcc attccttgcg gattacgaat gccataagac
     2041 cagatctcgg cgcgcgcacc ggattccttt ataaaaggat tatcatccgg gatttcgccc
     2101 tggtcggtca gacgcaccag tttgccctgt aatttatcca gatcctgcgc cgtcgggcgc
     2161 tgattgtttt cgcccagagc aataaaaaga taacctttac cgtcgaatac cagccgcccg
     2221 ccaaaatggt tgccggtaga cagttttggc atctggcgga agacggtgcg gaagtcggtc
     2281 acttttgaga gatcatcact taagcggcca taacccacag cagttccggc tttgccatca
     2341 tcgccaactt cggaataact taaccagatg cggcgagact gagcaaaatc aggcgctaaa
     2401 accacgtcca gcaggccgcc ctgcccgtgc gcccaaacgt ccggaactcc ggaaagcggc
     2461 gcagataatc cttttcctgc ttgccagtga cgcaactcgc cgccgcgcag agtgattaac
     2521 ataccgtgat tatcgggtaa aaaggccagt gcccagggat ggtcgagttt gtcttgcagt
     2581 acttcgacat ttaccgttgc aggagccgcc cagagagcgg aagaaagaca aataaggggc
     2641 acaaggaaaa aggattgtcg atgcataacg cctccggatt gactctggct taaagcgtag
     2701 tcagtggagg agataacaaa ttcattttta caaaaactta aacatgaagg gggagacgct
     2761 ttctccccct tagttttcag gccttctcaa gcatggcgtg cttctgcagg ctctggatac
     2821 tcagcgttaa gctcatcaga caattttcaa gcttatcggc gttgacggta ataacagtcg
     2881 ggcaatcatg gtgcccactc atcaaacata ctgcggctgt cgctaatgct tcttcagcat
     2941 gatgaagagc actccactct tcctgatcca gatgaagatt caaccgcagc gatttatcgt
     3001 gcagttcgcg attcagttta aaaaagttat ctcgtagatg attgctttcg ctgacggaca
     3061 tgtatccttt tgccttcctc agttgcacgt atgcagcgag gtcaacgcgt gcgtcgatca
     3121 accggttcag cagatcgatt cgctgtctgt caacgaacat acttcgatcc tcctctttcc
     3181 agccttgttg caattaaata gtatggctat tttttgagca aaatgctgtc caggatcaat
     3241 tcttcacgcc gaattaacaa aatttacatt tccttcgcct ccgccagcga atgagattgc
     3301 gatacaaatt ttatccagaa ctctgtagaa tccctgccct gacctttcct gtaactgaat
     3361 attttttaag ctatgagcaa agtaactccc cagccgaaaa tcggctttgt ttcccttggc
     3421 tgtccgaaaa accttgtcga ttcagagcgt attctcaccg aactccgcac tgaaggttat
     3481 gacgtggtac cgagctatga cgatgcgaac atggtgatcg tcaacacctg cggctttatt
     3541 gacagcgcgg tacaagaatc actggaagcc attggtgaag cgttgaatga aaacggcaag
     3601 gtaattgtga ccggttgtct gggggcaaaa gaagatcaga tccgcgaagt ccacccgaaa
     3661 gtgctggaaa tcaccgggcc tcatagctat gagcaggttc tggagcacgt tcatcactac
     3721 gtgccaaaac cgaaacacaa cccattcctg agcctggtgc cagaacaagg tgtgaaactg
     3781 acgccgcgtc attatgccta tctgaaaatt tcggaaggct gtaatcaccg ctgcaccttc
     3841 tgcattattc cgtctatgcg cggcgacctg atgagccgtc cgattggcga agtgttaagt
     3901 gaagcgaaac gtctggtaga tgcgggcgtt aaagagattc tggtgatctc gcaggatact
     3961 tccgcctatg gcgttgatgt taaacatcgt actggcttcc acaacggcga gccggtaaaa
     4021 accagcatgg tcagcctgtg cgaacagtta tcgaaactgg ggatctggac acgtctgcac
     4081 tacgtttacc cttatccgca tgtggacgac gtcatcccac tgatggcaga aggcaaaatc
     4141 ctgccgtatc tggacattcc gttgcagcac gccagcccgc gcattctcaa actgatgaag
     4201 cgtccgggtt ctgtagatcg ccaactggcg cgcatcaaac agtggcgcga aatctgcccg
     4261 gaactgaccc tacgctcaac ctttattgtc ggcttccctg gcgagacgga agaagatttc
     4321 cagatgctac tcgacttcct gaaagaagca cgtctggatc gcgttggctg ctttaaatac
     4381 agcccggttg aaggtgcaga cgccaatgcc ctgcctgacc aggttccgga agaagtgaaa
     4441 gaagaacgct ggaaccgttt catgcagctg cagcagcaga tttccgccga gcgcctgcaa
     4501 gagaaagtgg gccgtgaaat tctggtgatt atcgacgaag tggacgaaga aggcgcgatt
     4561 ggtcgcagca tggcagatgc accggaaatc gacggcgcgg tttatctcaa tggtgaaacc
     4621 aacgttaagc cgggtgatat cctgcgtgtg aaagtcgagc acgccgatga gtacgatttg
     4681 tggggtagcc gggtttaatt aattcaggcc gggcaaaatc atttgtccgg cctgcgaata
     4741 atgattactg ccagctaatc tgtgccctgc cgtttttctt actcttatac agccgctcat
     4801 ccgccagcat tttcaagcgt tcgacggtag tacattcatt tgaatgcgct acaccgatac
     4861 tggtgcttaa attaatgctt cccacctgga aatgaacgat ttcttttagt atcgatagca
     4921 actcttccag cgtgccgcca gtataaatca ccgcaaattc gtcacctccg aagcggtaga
     4981 ctttaccttt atctaccggg atctgtgatt taaggatttt caccactttc atcaacactt
     5041 catcgcccag caaatgaccg aaggtgtcgt taatgttttt gaatttatcg acgtcaaaca
     5101 gacacaaata accgcctttt tccggcagcg cttttaaatc ttcatcaaag gattttctgc
     5161 ccattgcttt ggtcaggtcg tccaggaacg ccgagttata catactgtag agttttttga
     5221 catcggcagc cgaaaagaaa agcccgccat ccagtacgcg ttcaaaatgg ttattaatga
     5281 tggcttcttt ctccacaata ccacgattaa tcactctgcg taccagatag aggcaactgg
     5341 cacccaatat cactgccacc aatgcggcaa agaaaataat caatgtacaa acccgcaaat
     5401 aaacatcttt tttatacgca aatacgtaca atccggtcgg caaataggcg aagtgctcca
     5461 tcgcttccga aacggtgaag atggcgtttt tagcattata ttcatcaccg gtcaccggtt
     5521 taccgttctt aaatagagag aacatcaggt tctcgctgtc catcaccagc gattttgaca
     5581 gtaaagaaaa acgctccaga ccaagcaaaa atatcagact acgattgtga gttaccggat
     5641 agaggtaata gacgaaggtt ttaccgtcaa aagagataaa ccctgtattt ttgacatgtc
     5701 gatacttcac aacctgcata atatctgcac aataattttt ctcgaactcc tttacctgaa
     5761 tacattcgtc actggaaatc agataaggac cgacatcgta caaataagag ggggaaaccg
     5821 agtcggcgta agctgtgcgc aaaccaacca acagctgttc ctccgtggga tttagcgtgt
     5881 tgataagatc aattgccacg ctacgccgat attcagcaat atttgccacc gcaaaagcag
     5941 tatcaataac ggactgtttc aggctataca cccgttcctt taccatttga gtgtagatcc
     6001 cgcaggcaac tgctgaaatc atgataaaaa tcagcagact gactgtaagt acgaacttat
     6061 tgattctgga catacgtaaa ctactctttt actaattttc cacttttatc ccaagcggag
     6121 aatggcatgg gtttctggta cagatacccc tgagcctgaa cacatcctaa tttaattaat
     6181 tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca ccgggatccg gttatagcgt
     6241 gccagatcga aaataatgcg cacaattttt cccgcagtac cgaaaatatc gccggtaaat
     6301 tgcgagtcca cctttatgct gtctggatta atttcataaa aacgtttcag gtttgacaaa
     6361 ccggtgccaa agtcatcaat cgatatccgt accccttgtt tacgcagttg ttcaatattg
     6421 agataaaaat gttcattaat tcgctcgatc acggtttcag tgatctcaac acagaggctg
     6481 ttcgccttca ggccattttc tttcatgatg gtggtaaaac gctcatgaaa gtgcggttcg
     6541 tttaactgcc agggcgaaac attaatatgc agtaagaaat catccttcag cgacgcacgt
     6601 tgactaaaat gtctgaatta acccattgcc gttttaataa tctgataacc cagctcattg
     6661 ataaacccga tatcttcagc aatggtaatg aacttcagcg gtgaaatgat cccccgctcc
     6721 gcagaaaccc aacggcacaa cgcctccgcg ctcagaatgg cgcgggtgtt aatatcaacg
     6781 atcggttgat agacgaggaa tagttcgcca atttccattg catggcgaat attacgcgca
     6841 atccagatat tctcttctat ttcgttgagt ttcgcgtcat cacagaaaat aaactcggag
     6901 aacatatggt cacggatact ggaaatcgcc agtttcagtt tttctgcgta actccaggcg
     6961 ttttccccct gcaaaggttc acaaatggca gacttatgga tgcgcagatt gcggtagcca
     7021 aagctgctgt caaaatcgtt taccatcgct attttacggg taaatgtctg tacattttcc
     7081 cgacacagaa gtaaatagag atcggcattg aatgtcacga tatcgcagca ttgcgcatat
     7141 ttctcggcaa ttcgctgaca caatttcctg acgattttat tgcgttcatc aacaccaaac
     7201 accgcctcca cggcttccag accacacagt ttgatgatcc ccacagccag atactgatta
     7261 ttgttggact caataatcgc catattttta ataaaaccgc tttcctgatc gtaagagatc
     7321 agtgagagca atttatttga ccgttcaatt tcggcatctt tcatttccgc gatcgaattt
     7381 ttgatgctga taatctcttt cgcatcgcct tcgcgaattt tcagagcctc gatctgatcc
     7441 gttgagtcgg gcaaaaacgc cagcgtatta accagctcgg tcatttcaga aaattgccga
     7501 cggaacacac gagtggtcat gaaatatacc accagcaaca cgataataat gaacgcaaac
     7561 ggtagataaa aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag
     7621 atttcgaaca atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca
     7681 gaatcaataa tattataatt accttcgcta ttataaataa tcagtttttc acgagcgagt
     7741 tgattcttgg caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg
     7801 atttgatcaa gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc
     7861 gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc
     7921 ggacgatcaa agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc
     7981 gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca
     8041 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa
     8101 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
     8161 tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag
     8221 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
     8281 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
     8341 atcatgaaaa aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
     8401 atcttttcgt ataaactcag catgttaata catttaacaa aataactatc tgaacgctgt
     8461 gccgctgcaa aaatagtccc ttgctgcggc tttttaacca aattacccga ttctacaaaa
     8521 tcagcagaaa gttacaacac ttgcttaggg caataatttc ctgtgaggtg attacccttt
     8581 caagcaatat tcaaacgtaa ctatccttta attttcggat ccagcgcatc gcgtaaacca
     8641 tcgcccaaca aattgaacgc cagtacggtc agaaaaatag ccagggccgg aaaaacagcg
     8701 acatgcggcg cgataaccat atccgctcgt gcctcattga gcattgctcc ccactctggt
     8761 gtcggcggct gcgcaccgag gccgagaaat gacaggctgg cggcagagat aatcgaggtg
     8821 ccaatgcgca tggtgaaaaa caccacgata gaagagacgg tcccaggcag gatatgacgc
     8881 aataaaatgg tcatatcgct ggcaccaata ctgcgtgctg actcaataaa ggtttgctgt
     8941 ttcaacacca gcgtgttgcc gcgcaccagg cgggcaaacg cggggatgga aaaaatggcg
     9001 actgcgataa tcacgttagc aatgccgctt cccaacaccg caacaacagc gatcgccagt
     9061 aaaatacccg ggaaggcaaa cagcacatcg caaatgcgca tgatcagccg atcccaccag
     9121 ccttcataat atccagccaa caagcccagc aacgtcccta tcgccacacc gataaacacg
     9181 gcaaacacgc ccgccgccag cgagatttgc gcaccaacca ggacacggct gaaaatgtcg
     9241 cgccccagtg aatcgacgcc aaaccagtgc tgcaaagaag gtccgttatt cagattgtca
     9301 taatcaaaat aattttcggc gtcataggga gcgatccagc gtgcaaaaat ggccaccaca
     9361 atcaataaaa taacgaataa tgcggcggtc atcgccatat gctgacggcg aaatcgtcgc
     9421 cagaattcat gccacggtgt acgtacctgg tcaggtttga ccagtggcat ggcgtttaac
     9481 accgcctgac gtcgccagtt aaatagtcgc atccttactt gtacctgata gccgggttaa
     9541 tggcggcgta aagcacatcc accactaagt tgataagaat aaattccagc gagaaaagca
     9601 gaatttccgc ctgaatcacc gggtaatcgc gcatttctac agagtcaacg agtaagcgtc
     9661 caagtccggg ccagttgaaa actttctcca ccacgataga accaccgagc aaaaagccaa
     9721 actgtaagcc catcatggtc actaccggga tcatcgcgtt gcgtagcccg tgtttgagga
     9781 caacccaggt ttcgctcacc cctttcgccc tcgcggtacg catataatct tcgcttaaaa
     9841 cgtcgacaaa tgacgcacgg gtaaagcgcg ccatcacggc ggccactgcc gcgccgagcg
     9901 tcagggaggg taaaatgtag tgctgccaac tgtctgctcc cacagtaggc agccagccca
     9961 gttcaacgga gaatacctga attaaaaaca tccccagcgc aaatgccgga aacgagatcc
    10021 ccgacaccgc aatggtcata ctcaatcgat ccggccaacg attacgccag acggcggcga
    10081 taattcccgc cgccatacca aatatcaccg cccagaccat actggttatg gtcagccata
    10141 gcgttggcat aaagcggctg gcaatctcat cggcaaccgg acgacgcgac accatcgaca
    10201 ggccaaaatc cccctgcaca gcattgctga tatagtgcca gaactggtga tacagcggct
    10261 gatccaaccc cagctgctga cgcaccagtt ctataacctg cgcatcagct tcgggcccgg
    10321 caatcaatcg cgccggatcg ccgggcagca tgtggacaaa taaaaacacc agcaccgaga
    10381 cgataaacag cgtcggaatc aaccccagta atcgtttgat aacgtaataa agcattccac
    10441 tccctgcgtt gcttattgca aatccgcgtc ttcaaagctg aagccggtat ctggcatgat
    10501 ccaaaaaccg gtcaggtttt tactgtgtgc cgacaccagt ttttctacca ccagcgggat
    10561 ccacggcgat tcttgccaga tgatatcctg cgccgcctta tataagcggg tcttttccgc
    10621 cggatcattc gttttcagtg cctgagccag gaagtcatcc acctgtttat tgctgtaaaa
    10681 cgcggtatta aacagcgtcg gtggccagtt ttgcgaggca aacagcggcg atagtgccca
    10741 gtcggcttcg ccggttgaag ccgaccagcc agtgtagaac atccgcacgc cactctcttt
    10801 ttgcccttta ccttcaactt ctgccgcccg ctgtccggca tccatcgcgg tcacctgggc
    10861 tttaatcccg acctgcgcta actgctgctg ggtaaattgc agcactttct gcgcggtgct
    10921 gtggttatgt gacgaccaca gcgtggtact gaaaccgttg ggatatcccg cctctttcag
    10981 taattcgcgc gctttcactg gatcgtaagg ccacggttta taactttgcg cgtaggcgat
    11041 acttggcggt accacaccag tagctggagt tgcatagccc gcaaaggcaa ctttcaccag
    11101 cgccggacgg ttaatggcgt aattcagcgc ctcacggacc ttcgggttat cgaacggctt
    11161 ttgcgtcacg ttcatactga tataacgctg cataattgac ggactggcca tcaactcgat
    11221 atttttgttt ttctccagca gtgtggcctg ctcgtaggga atggggaaag caaactgcgc
    11281 ttcaccggtt tgcagcattg ccgcgcgggt gttgttatcc gccaccggac gccaggttat
    11341 gctgtccagt ttgggcaatc ctggctgcca gtaacccgcg aattttttca ccttcacaaa
    11401 atcggtctga ttccaggtat ccagttcata cggtccggtt cccaccggat gaaaaccaat
    11461 ctccttgcca tatttttcca gcgctgtcgg tgaaatcatc gcggtcgccg gatgggcaag
    11521 aatattaata aacgctgaga acggctgttt gagggtaatc tttaccgttg tcggatcgat
    11581 cgcctccgtt ttggcaatat tcttatacag gttatagcgt ttaagatgat tcgccggatc
    11641 gctggcccgg tccagattcg cttttaccgc cactgcgttg aaatcggtgc catcctggaa
    11701 tttaattcct tcccgcaatt tcacggtgta agtaatgccg tcatcggaaa cggtataact
    11761 ctccgccagc acgtttttca gtttcatctc tttatccaga ccgaacagcc cctggtaaaa
    11821 cgatttcgct acggcctgag ataacgtgtc attcgcgtca tacggatcga gcgtggtgaa
    11881 attcgatcct accgccacca ccacatcttt ggcagcgaat gcacaagatg ccatcaacgc
    11941 tgtcgcaatg cccagcgcca ctaacccact acggtgtaca gctcttgcca ttttattctc
    12001 cgcctgaatg ttatctacgc atgaatgcgt attctgattg tggttgtgcg acgtaatgcc
    12061 ccggcccgac gcattgcaac gagacggctg ccacctcttc gccacgcaga tgaatattgc
    12121 tgggaagatc gtccgacagc agtacacgct gcggtcgttg tcgggacggt tcagcgaccg
    12181 gaactgccgc cagtaattta cgcgtataag gatgctgcgg gttttcgaag accgcgcacc
    12241 gtgaaccaat ttcaacaatt tgcccgagat acatcaccgc cacacgatga ctaatccgct
    12301 ctaccacagc catatcgtgg gagataaaca gatacgcaat gccgaaatca cgctggagat
    12361 cgagcaacaa gttgataatc tgcccgcgaa tagaaacatc cagcgccgaa acggcttcgt
    12421 cggcaatgat cacttttgga ttcaatgcca acgcgcgagc aatgcagatg cgctggcgct
    12481 gaccgccgga aaactcatgc gggtaacgcc aggcatgttc aggtaacagg cccacgcgct
    12541 ccagcaacca cgcaacgcgt gcagccgcgt ctttacctgg caataaaccg tgtacacgca
    12601 gcggttcgat aatcgaatca ccgatggtct gacgtgggtc cagcgaagcg taagggtcct
    12661 gaaaaataaa ctgaatatcc cggcgcaatg cctgaagttt gccaggtgac aaggtatcga
    12721 ttcgctgacc gttaaagata atttcgccgc cctgcgattc gaccaggcgc agcaacgccc
    12781 gcccggtagt ggatttaccg ctgccagact cgcccaccag cgatagcgtt tcgccaggcc
    12841 agagatcaaa actgactttc tcaacggcat gcacttcccg cgttacgcga ttcaacaaac
    12901 cgctgcgcaa agggaaacgg gtgacaagat tacgcacccg taaaacaggt tcgccatcca
    12961 ccaccgtttt ctgctcgatg gggggttcct gtttcgctgg atgttcaagc gatatcaatg
    13021 ggaaacgtcg gggataatct aaccctttca tcgcaccaag ttgcggaaca gcagctaaca
    13081 gcgcacgggt gtaaggatgt tgcggtgcat gaaaaatctg ttcgacggta cccgtttcca
    13141 ccgcctcgcc ctgatacatc accagtaccc gatcggcaat ctctgccacc acgcccatat
    13201 cgtgagtgat aaagataacg cccatcgaca tctctttttg caatactttg attaattgca
    13261 ggatctgcgc ctgaatagtg acatccagcg cggtggttgg ctcatcggca atcagcaccg
    13321 ccgggcggca tgacagcgcc atcgcaatca tcactcgctg gcgcatcccg ccagagagtt
    13381 gatgcgaata acgtgaaaga atggtttgtg cctccggaat gcgtacctga tccagcatcc
    13441 gcttcgcctc gaccatcgct tcttcacgac tggcgttctg atgcagacga attgattcgg
    13501 caatctgttc acccacagta aatactgggt tcagcgatgt catcggctcc tgaaatatca
    13561 tcgccatatc cgcaccgcgc acatggcgca tttgtgcagc gctctgctcg ctaagttcaa
    13621 tcacatcacg actgcgccgc cgcaacagca ttttatcgca ctgtactaaa ccgcccgcct
    13681 gttccaacag acgcatcaat gccagcgcag tcactgactt accggagccg gattcgccta
    13741 caattgccag cgtctcaccg cgttgcagac taaaagagag attgcggacc gcagctattt
    13801 tctgctggtc ctgcataaag gcaatattaa gattttcaac cgccagcaca ttaccggcat
    13861 caagttcatc actgtgtggc aacggtgtcc cctttttcac ggtagatacc ggtggttggc
    13921 gtatcgcctg cgtagcccca ggcgcgatac attccttcgg tgttaaacgg tagcgcgaca
    13981 ttcccttcat ggtcgatagc gattaagcca ccgctaccgc caagcgcagg gagtttttcc
    14041 attactaccc gctcgcaggc ttccgcgaga cttaatccgc cgtaatccat taacgcggcg
    14101 atgtcatatg ccgccagcgc gcggatgaag acttcgcctg tgccggtaca agaaaccgcc
    14161 acactggcgt tattggcgta gcatccggca cccactaagg gactatcgcc aactcgtccg
    14221 ggtaatttat tggtcattcc gcctgtggac gtggctgccg ccagattgcc gtctaaatcc
    14281 aacgccacgg cccccacggt gcccattttc tgtttttcat ccagtggcgc accgctatgg
    14341 tcgaggactg ttgccccttc ctcgcgcgct gccagtagtt gttcataacg caaaggcgtg
    14401 gagaaaatct ccggtgagac gcgctccatg ccatgagcaa acgcaaaatt ttctgcccct
    14461 tcgccaatca tcatcacatg cgggctttgc tccatcacca gccgggcggc aagaaccgga
    14521 ttacgcagat gactaacgcc cgccaccgca ccggctttca gggtgttacc atccatcaca
    14581 caggcgtcca gttcatgggt ttcatcacgc gtaaagacag cgccaattcc ggcgttaaac
    14641 agtggacact cttccagcag acgcaccgct tccgtcacca catccagcgc actttcgccg
    14701 gcttccagca ttttctgccc ggtttcaaca atggcagaca acgcctcgat gtagcgtaat
    14761 tcctgttgca gactcatctg cgcgcggcta attgcacctg cgccaccatg aattgcaatg
    14821 actgctttgc ccatagtatt cgtccattaa gggtggctcg ccacttttgc tataaatatc
    14881 agaattttta tgaattactc attcaatttt tgaatataga aagatggata tggcatgtaa
    14941 aggcaggagc caacgctacg cccgaggtgg catacagtct atggcgtttt tctgacataa
    15001 taggcaaatt cgattttgcc tccgcaggag tgttttcatg gaatttacca ccggattgat
    15061 gtcgctcgac accgcgctta atgagatgct ttctcgcgtt accccactga ccacccagga
    15121 aacgctgcca ctggttcagt gttttggtcg tattctggcg agcgatgtcg tttcgccact
    15181 ggatgtcccg gggtttgata actccgctat ggacggctac gcggtgcgtt tagccgatat
    15241 tgcctccggg caaccgctgc ccgttgccgg taaatccttt gccggtcagc cataccatgg
    15301 tgaatggcct gcgggtacct gcattcgtat tatgaccggt gcgccggtgc cggaaggctg
    15361 cgaagcggtg gtgatgcagg agcagactga acaaacggac aatggcgtgc gttttactgc
    15421 tgaagtgcgt agcgggcaaa atattcgccg tcgcggtgaa gatatctctg caggtgcggt
    15481 tgttttcccg gcgggaactc gcctgactac cgcagagctg ccagtaattg cttcactggg
    15541 gattgccgaa gttccggtga ttcgtaaagt gcgtgtagcg cttttttcta ccggtgatga
    15601 actccagttg cccggtcagc cgctgggcga cggccaaatc tacgacacca accgtctcgc
    15661 cgtacacctg atgttagaac agctgggttg cgaggtaatc aacttaggga ttatccgcga
    15721 cgatccacat gccctgcgcg ccgcatttat tgaagccgac agccaggcgg atgtagtgat
    15781 cagttccggc ggtgtttcag tgggtgaagc ggattacacc aaaatgattc ttgaagagct
    15841 gggggagatc gccttctgga agctggcgat taaaccaggt aaaccgttcg cgttcggtaa
    15901 actcagcaat agctggttct gcggcctgcc gggcaacccg gtttcagcga cgctgacctt
    15961 ctatcaactg gtacagcctt tgctggcaaa actaagcggc aacaccgcca gcggcctgcc
    16021 cgcgcgccag cgcgtacgca cagcgtcccg cctgaaaaaa acgccaggac gtcttgattt
    16081 ccagcgcggc gtgctgcaac gcaacgccga cggtgaactt gaagtgacga ccaccggaca
    16141 tcagggttca catatattta gctcctttag tctcggcaac tgctttatcg tgctggaacg
    16201 cgatcgcggc aatgtggacg tgcgcgaatg ggtggaagta gaaccgttta acgcgttgtt
    16261 cggaggcctg taatggcgga actcagcgat caggagatgc tgcgctacaa ccggcaaatc
    16321 attctgcgcg gttttgattt tgacggtcag gaggcgctaa aagattctcg tgtgctggta
    16381 gtcggcctcg gtggactcgg ctgcgcagct tcgcagtatc tggcaagcgc cggtgtcggc
    16441 aacctgacgc tgctcgactt cgacacggtt tcgctctcga atctgcaacg ccagacactg
    16501 cacagtgatg ccacggtcgg gcaaccgaag gtggaatccg cccgtgacgc cctgacgcgg
    16561 atcaacccac atatcgcgat tacgccagtc aatgcactgc tagatgacgc agaacttgca
    16621 gcattgattg ctgaacacga tctggtgctc gactgtacgg ataacgttgc ggtacgtaat
    16681 caactgaacg caggctgttt tgccgcgaag gtgccgctgg tttccggcgc ggcaattcgt
    16741 atggaagggc aaatcacggt cttcacttat caggacggcg aaccgtgcta tcgctgcctt
    16801 agccgtttgt ttggcgagaa cgcgttaacc tgcgtagaag caggcgtgat ggcaccgttg
    16861 ataggcgtaa ttggttcgtt gcaggcaatg gaagcgatca aactgctggc aggttatggc
    16921 aaaccggcca gcgggaaaat cgtcatgtac gatgcgatga cctgtcagtt tcgcgaaatg
    16981 aaactgatgc gcaatccggg gtgtgaggtg tgcgggcagt aattgccacc tccaagcctg
    17041 ataagacgcg acaagcgtcg catcaggcat ttgctccaga cgccagatgc ggcgtgaacg
    17101 ccttatctgg cctacggttc gggcacagat ttccaagcgc ggcaggcatc ggtgcactcc
    17161 gccacgtcat cagcaattta tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa
    17221 acgctccctg ccagtcctgc tcaaacttcg tcacagcggc ttccaccgcc ggatagctaa
    17281 tcatctgttg tgccacatcc agtggcagag taattgattc acatccggcc agtaagcagt
    17341 ccagcgcctg acgcggggtt ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca
    17401 ttttcaataa ctggtgtaag tcggtcacag tctgaatgcc gctaccgccc tgagcatcaa
    17461 tacgattaac gtaaggcgca acatattccg cacctgccag cgccgacagc agcccttgtg
    17521 ccgcgccata taccgcggtt cccagcgtcg gaatcccttc cgcttttaac atcttaatcg
    17581 ctgccagccc ctcggctgtc accggaactt tcaccacgat atccgcaata atagaacgca
    17641 gcttaagcgc gtcattcacc atcccttcag cagtggtagc cattacctgg gcaaacagac
    17701 gcccctgacc gcccatcgct tcatgaagtt gcggaagcac aacatccagc ggttttttac
    17761 ccgcggcgat aatgcttggg ttagtggtca cacccgccag cggaaaaata cgtgacagcg
    17821 ccttcaccgc aacaacgtct gaagtatcca gatacagttc cataaccatc ctcaaaatgt
    17881 actttttgtc tgaatgtaga ctatcacggg aaaactctct gaacagttga cgcacatcaa
    17941 tctaactttc attcgaaagt attttaatgt ttgaacgaaa cagagaggca actatgattt
    18001 tcaatattca gcgctactcg acccatgatg gccccggtat ccgcacggtc gtatttctta
    18061 aaggctgttc gctgggctgc cgctggtgtc agaacccgga aagccgcgcc cgtacgcagg
    18121 atctgctgta tgacgcacga ctgtgtctgg aaggctgcga gctgtgcgct aaggccgcgc
    18181 cggaagtgat tgagcgcgcg ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg
    18241 agcatctgac ggcgttaacc gactgctgtc cgacacaggc gttaaccgtg tgtggtgaag
    18301 tgaaaagcgt tgaggagatc atggcgaccg ttctgcgcga taaaccgttt tacgatcgca
    18361 gcggcggcgg cttaacgctt tcgggtggtg aacccttcat gcagccggaa atggcgacgg
    18421 cgctgctgca agcctgccac gaggcaggca ttcatactgc ggtagaaacc tgtctgcatg
    18481 tgccgtggaa atatatcgcc ccttctctgc cctatatcga tctgtttctt gccgatttaa
    18541 aacacgttgc cgacgcgccg tttaaacagt ggaccgacgg taacgccgcc agggtgctgg
    18601 ataacctgaa aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc
    18661 agggctttaa tgccgacgaa acctctgtaa aagccattac cgattttgcc gccgacgagc
    18721 tgcacgttgg cgaaattcat tttctgccct accacacgct gggcatcaac aaatatcact
    18781 tacttaatct gccctatgac gccccggaaa aaccgcttga tgcgccagag ctgctcgatt
    18841 ttgcccagca gtatgcctgc cagaaagggt taaccgcgac cttacgagga taacaaccat
    18901 gaccacactg aaactggaca cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt
    18961 gcatattgtg aagccgccag tctgtaccga gcgcgcgcag cactataccg agatgtatca
    19021 acaacatctc gataagccga tcccggtacg tcgcgcgctg gcactggcgc atcacctggc
    19081 gaatcgcacc atctggatca aacacgatga gttgatcatt ggcaaccagg caagcgaagt
    19141 tcgcgccgcg ccgatcttcc cggaatatac tgtctcgtgg atcgaaaaag agattgatga
    19201 tctggcagat cgtcccggtg ctggctttgc ggtgagcgaa gagaacaaac gtgttctgca
    19261 tgaagtgtgc ccgtggtggc gcggtcagac ggtacaggat cgctgctatg gcatgtttac
    19321 cgatgagcaa aaaggtctgc tggcgaccgg catcattaaa gcggaaggca atatgacctc
    19381 cggcgatgcg cacctggcgg tcaatttccc gctgctgctg gaaaaagggc ttgatggtct
    19441 gcgtgagaaa gtagcggaac gtcgctcgcg catcaatctg acggtgctgg aagatttgca
    19501 cggcgaacag ttcctgaaag cgattgatat catgctggtg gcagtcagtg aacacattga
    19561 acgtttcgct gccctggcgc gtgcaatggc cgcgaccgaa acccgcgaaa gccgtcgcga
    19621 tgaactgctg gcgatggcag aaaactgcga tcttatcgcc caccagccgc cgcagacttt
    19681 ctggcaggcg ctgcaactgt gttacttcat ccagttgatt ttgcagattg aatctaacgg
    19741 tcactcggtg tcgtttggtc gtatggatca gtatctctac ccgtactatc gccgcgacgt
    19801 tgaactgaac cagacgctgg atcgcgaaca cgccatcgaa ttgctgcata gctgctggtt
    19861 gaaattgctg gaagtgaaca aaatccgctc cggctcccac tcaaaagcct ctgcgggaag
    19921 tccgctgtat cagaacgtca ccattggcgg acaaaatctg gttgatggtc aaccaatgga
    19981 cgcggtgaat ccactctctt acgcgatcct cgaatcctgc ggtcgcctgc gttccactca
    20041 gcctaacctc agcgtgcgtt accatgcagg aatgagtaac gatttcctcg acgcctgcgt
    20101 acatgtgatc cgctgcggct tcgggatgcc ggcattcaac aacgacgaaa tcgtgatccc
    20161 ggaatttatt aaactcggta ttgaaccgca ggacgcttat gactacgcag cgattggttg
    20221 tatagaaacc gccgtcggtg gcaaatgggg ctatcgctgt accggcatga gctttatcaa
    20281 cttcgcccgc gtgatgctgg cggcgctgga aggcgggcgt gatgccacca gcggcaaagt
    20341 gttcctgcca caagaaaaag cgttgtcggc aggtaacttc aacaacttcg atgaagtgat
    20401 ggacgcgtgg gatacgcaaa tccgttacta cacgcgtaaa tcaatcgaaa tcgaatatgt
    20461 cgtcgacacc atgctggaag agaacgtgca cgatattctc tgctcggcgc tggtggatga
    20521 ctgtattgag cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact gggtttctgg
    20581 cctgcaggtc ggtattgcca acctcggcaa cagcctggcg gcagtgaaga aactggtgtt
    20641 tgaacagggc gcgattggtc agcaacagct ggctgccgca ctggcggatg acttcgatgg
    20701 cctgactcat gagcaactgc gtcagcggct gattaacggt gcgccgaagt acggcaacga
    20761 cgatgatacc gtcgatacgc tgctggctcg cgcttatcag acctatatcg acgaactgaa
    20821 acagtaccat aacccacgct acggtcgtgg tccggttggc ggcaactatt acgcgggtac
    20881 gtcgtctatc tccgctaacg taccgtttgg cgcgcaaact atggcaacgc cggacgggcg
    20941 taaagcccac accccgctgg cagaaggcgc tagtccggcc tctggtactg accatctcgg
    21001 ccctactgcg gtcattggct cagtgggtaa actgcctacg gcagcgattc tcggcggcgt
    21061 gttgctcaac cagaaactga atccggcaac gctggagaac gaatctgaca agcagaaact
    21121 gatgatcctg ctgcgtacct tctttgaagt gcataaaggc tggcatattc agtacaacat
    21181 cgtttcccgc gaaacgctgc tggaggcgaa aaaacatccg gatcagtacc gcgatctggt
    21241 agtgcgtgtc gcgggttatt ccgccttctt caccgcgctc tctccagacg ctcaggacga
    21301 tatcatcgcc cgtactgaac atatgctgta atctcttcgg cccggctgcg gcgtcgccgg
    21361 gctaaatcat tcacatcatc aatttcatcc ttactttcat tcgaaatata atttgtgctc
    21421 tgcgtcacat tgttattaca ctgttacggt ttcattttcg agccaggagc acacctatga
    21481 gcgtaaaagt tatcgtcaca gatatggacg gtacttttct taacgacgcc aaaacgtaca
    21541 accaaccacg ttttatggcg caatatcagg aactgaaaaa gcgcggcatt aagttcgttg
    21601 tcgccagcgg taatcagtat taccagctta tttcgttctt tcctgagcta aaggatgaga
    21661 tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg
    21721 gcgaactgac ccgacatgaa tcgcggattg ttattggcga gttgctaaaa gataagcaac
    21781 tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat
    21841 ttgtcgcact gatggcaaaa cactaccatc gcctgaaacc tgtaaaagat tatcaggaga
    21901 ttgacgacgt actgttcaag ttttcgctca acctgccgga tgaacaaatc ccgttagtga
    21961 tcgacaaact acacatagcg ctggatggca ttatgaaacc cgtcaccagt ggttttggct
    22021 ttatcgacct gattattccc ggtctacata aagcaaacgg tatttcgcgg ttactgaaac
    22081 gctgggatct gtcaccgcaa aatgtggtag cgattggcga cagcggtaac gatgcggaga
    22141 tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa
    22201 tcgcccgtta cgctaccgat gataataatc atgaaggcgc gctgaatgtg attcaggctg
    22261 tgctggataa cacatcccct tttaacagct gacctctcta ccggagccat gcgctccggt
    22321 tttcccttct ttttacatcc tgtacatcac atcaaatcat taaacttgca ttaacttatt
    22381 tataacttgt attgtcacat gcaaattagc atagtttcga atgaaagtta atgcaaggtg
    22441 gttatggctt ctacttttac cagcgacaca ttgcctgccg atcacaaagc agctatccgt
    22501 cagatgaagc acgcgctgcg ggcgcagctt ggcgatgtcc agcagatctt taatcagcta
    22561 agcgatgaca ttgccacgcg agtggctgaa atcaacgcac tcaaagcaca gggcgatgcc
    22621 gtctggccgg tgctgtctta tgccgatatc aaagcaggtc atgttactgc agagcagcgc
    22681 gaacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc atttcccccg cgaacaagcg
    22741 ctaggctggg atcagtcgat gctggactat ctgaaccgca accgctttga cgaggtctac
    22801 aaaggccccg gcgataattt cttcgggacg ctcagcgctt cacgtcccga gatttacccc
    22861 atctactggt cgcaggcgca aatgcaggcc cgccagagtg aagaaatggc gaatgcgcag
    22921 tcgtttctca atcgtctgtg gacatttgaa agtgatggaa agcaatggtt taacccggat
    22981 gtgagcgtca tctaccctga ccgtatccgc cgccttccgc ccggaacgac ctccaaaggt
    23041 cttggagcgc ataccgactc cggggcgctg gaacgctggc tgcttccagc gtatcagcac
    23101 gttttcgcca acgtctttaa tggcaatctg gcgaaatacg atccctggca tgcggcacat
    23161 cgtacggaag ttgaagagta cacggtggac aacaccacca aatgttccgt gtttcggaca
    23221 ttccagggct ggactgcgct ctcggatatg ctgcctggtc aggggttgct gcacgtcgtg
    23281 cccattcctg aagccatggc gtacgtactg ttacgtccgc tgcttgatga tgtgccggag
    23341 gatgaactgt gcggcgtagc gcccggaaga gtgttgccgg tatcagagca atggcatcca
    23401 ctgttaattg aggcgttaac cagcattcca aaactcgagg ccggagactc cgtctggtgg
    23461 cactgcgacg tcatccattc cgttgccccc gttgaaaatc aacagggttg gggcaacgtg
    23521 atgtacattc ctgcggcacc gatgtgcgag aaaaatcttg cctacgcgca caaggtgaag
    23581 gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc gcgaggacta tgaaacaaac
    23641 tgggaaggac gctttacgct tgccgacctc aacattcacg gtaagcgagc gttgggcatg
    23701 gatgtttgag agttaatgga agtgaattga tgtgggatta agaaaatcga ttcgacgtta
    23761 ctcagattgc tgacaagtgc gctttcttaa tgcctgatgc ggcgttaacg ccttatccgg
    23821 cctacaaagt cctgcagatt caatatatta caggtactgc gtaggcctga taagcgtagc
    23881 gcatcaggct attttgcatt tgtcatcagc cgtcgtcacc ccgacagtat cggggtgacg
    23941 gataatcact tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc
    24001 gatcacgcgt tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg
    24061 gtcgtgtgaa acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc
    24121 gatggattcc atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg
    24181 catcattaac ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg
    24241 ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc
    24301 gtcatcgcct tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa
    24361 ctcatattcg tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt
    24421 gccgctgtcc ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc
    24481 gttggtaccc agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa
    24541 cagtggaccg ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag
    24601 tttcttatcc tgttcaaaac ggataaacgg gttctgacgg ctggaggctt tcacctcttc
    24661 cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt
    24721 ggcgctaaag cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt
    24781 atcggccagc agacgttcac gcgcttgggt cgccgccgtc atgtactcat cgtagttacc
    24841 cggataaacg cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag
    24901 gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc
    24961 cagccagcga atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc
    25021 cggctcagca aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc
    25081 actcatcggg ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc
    25141 gcgagcttcc gcagagtaac cgtccatttc gccgtatttc acttccagat cggccacttt
    25201 atagccgtct tcttcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac
    25261 ttcccacaac tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc
    25321 aaactgatcc tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccag
    25381 cgtcggctca aggtcgccgc cgagaatctt cataaaggtg gatttaccac taccgttcgc
    25441 gccaatcagg ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg
    25501 cttactgccg aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga
    25561 gatatgacaa accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag
    25621 gtctacactc aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag
    25681 ctggaatgaa cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca
    25741 ggcacaaaat ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg
    25801 ctgttgtcgg cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga
    25861 gtcgcctggt tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg
    25921 agtattttgc cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg
    25981 tggatacctt cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc
    26041 cggataccgt tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc
    26101 cgaaagggac caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata
    26161 cgcctatcaa ctggaccacc aaagttgagc ataagaaagc aggcccgacc tggacgccga
    26221 ccgccaaaat gcacgcagag taccgcgctg cgggcgaacc gctaccggct gtcgttccgg
    26281 caggtccgga taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc
    26341 atggcaccaa cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc
    26401 gtaacgaaga catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta
    26461 ttgatgagcc ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata
    26521 acccgctgtc taccaccgaa gcccagtttg aaggtcagga aatcgtgcca attaccctga
    26581 cgaagagcgt gcagacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag
    26641 cgatcaaaaa tcgttccggg atgccggttc gcctgaatta atcgtcaaaa cgtaaaacaa
    26701 aggcggactc ataatccgcc ttttttattt gccagacctt accaggccgg gatcataact
    26761 aacaaaacgt atcccaccag cgccgcccac aacagcatcg gtattgaata gagatggaaa
    26821 cgccaccaga tgcggcgatc gttcgccata cgtagcgcaa tcaaatttgc cagcgatcct
    26881 ggtaataaac caaagccccc cacgtttacc gcccatgcca gtaataaaga cggaggtaca
    26941 taattgagca gcaaaatggt actcggcacg ttactgatca cctgcgataa accgactgcc
    27001 gttaaccata acccgggttc agataaatga ctcacgttac ccaacacgcc ttgcaacgct
    27061 ggaagctggg tcagtaaatg gacgtcgata aacatcgcca taaacaccag cagcaacgtc
    27121 cagtccacac tcaacaccac gcgacgcgcc agcagcacaa agcccgccgc cacaatcacc
    27181 agtccccaca gctcttgttt gaactccagc gccgtcagaa agacgatata cagccccaga
    27241 caattccaca ccagccgcgg tttccactcc ggtgtttgca cccccgtatg gtattgcagc
    27301 gcctttccag ggaaacaaca ccagcacaac agcaggagtg tcagcatcat tgcgccagcc
    27361 atcggtgcca tttgggcaat aaatccggca aacgaaagac cagaacgtcc ccagataaga
    27421 atattttgcg ggttgccaat tggcgtcagt agcgaaccag cgttgactgc cagcgcctca
    27481 aaaataatca gccgattaac cgggatctca cacagtcttt ttagcgtgat agtcagcgga
    27541 acaacaataa acagcgcgac atcgttggtc agaaaggtag aaagcagcgc cgccgccagc
    27601 accataaaca tcgccagccg acgctccgta gcaaagcggc gcaccatttt gcgccccagc
    27661 acatcaaaat aaccgcttaa ctccacacct ttggtcagca gcatcaggcc gcttaaggtg
    27721 atgatggtgt gccagtcgat agcagcaggc caggatttcg gtgcaaaggg cacgaaaaag
    27781 cttaatccga taccaacaag aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt
    27841 aaaaaaggca ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg
    27901 tctcttcact aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt tccggactga
    27961 cgcccaacac caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca
    28021 gcttctctcc ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca
    28081 tggttgccag ccgcttaagc attttagcca ccgtcggttg cgaaactccc agacgagcag
    28141 ccatgtccac ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt
    28201 aatcatcaat aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct
    28261 cttccacgtt cactaactgc gtcacttttt ttgctattgg cgtacctgcg cgacgactca
    28321 ttgtgcttcc tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa
    28381 accagagttg cgaaggtaca aaaaattaac gttttagcaa tagctatata atatagcctg
    28441 tgctatatct gtatgtaatg caatcatccc tcaaggatcg acgggattag caagtcagga
    28501 ggtcttatga atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta
    28561 cggttaatgc tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaagataaa
    28621 ccttcagata aatagcggcg tcgcggtacg ccgcttcact cctgctttta tgcaggcata
    28681 acgcgttttg gtctgaaaaa aaccactttt tgtcggattt gcaatcccct tcgcaaaaga
    28741 tttgttcgtc agtagttgac ctgaacggcg gctcgctcta tcttcttgca gccctgcgta
    28801 tattgcggct cgcggatgcg gacccctttc ccctcttcac gcactcttgc aggtattgac
    28861 ccttgacgcc agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct
    28921 caaagaatcg cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt
    28981 acagtcgcta ttaattaacc tcggcttagg ttaccccttc agtttgctct acaccgctgc
    29041 gtttacggct attttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt
    29101 cggtgtcagt tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc
    29161 taatttcaat acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct
    29221 gacgattttt ccgtggtaca gctacctggt cggcttattt atttttgcgc tcggcgtaat
    29281 agcaatcagg cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg acagcctgtg
    29341 tctggtattc agtgtggcga cattttttgt tgctcccgtg caaaacctgg cctggggtgg
    29401 cgtatttaaa ctgaaagata ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt
    29461 caataataac gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga
    29521 tacctggacg gtcactgccg ttaagccgaa gtatcagacc tatgtggtag tgatcggtga
    29581 aagcgcgcgt cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc
    29641 cagcagcgtt aacggtttga tatttgctga ctacattgct gccagtggct ccacgcagaa
    29701 atcgcttggc ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc aggataactt
    29761 tgtcaccctg gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggccaaat
    29821 cggcgaatac gatactgcta tcgccagcat cgccaaacgt gcagatgaag tgtacttcct
    29881 gaaagaaggt aattttgaag cagataaaaa cacgaaagac gaagcgttac tggatatgac
    29941 cgctcaagtg ctggcgcaag agcactcgca accgcagctg attgttctgc atctgatggg
    30001 ctcgcatccg caggcctgcg acaggacaca aggcaaatac gaaacctttg tgcaatcgaa
    30061 agaaacgtcg tgctatctct ataccatgac gcaaacggac gatttactgc gcaagctgta
    30121 cgatcagtta cgcaacagcg gcagcagctt ctctctggtt tacttttctg accacggtct
    30181 ggcctttaaa gagcgcggca aagacgtgca ataccttgcc catgatgata aatatcagca
    30241 aaatttccag gtgcctttta tggtcatttc cagcggcgat aaagcgcatc gggtgattaa
    30301 agcccgccgc tcagccaatg acttcttagg ctttttctcg cagtggacgg gaattaaagc
    30361 gaaggaaata aatatcaaat acccgtttat atctgagaag aaagccgggc cgatatacat
    30421 caccaacttc cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc
    30481 gaaaccttaa aaacaaaaat ccgccccgag aggcggattt ttatatcacc aaagtgatta
    30541 gaagcggtaa ccaacaccgg caatccaggt gcctacgtca acgctacgaa tacggctctg
    30601 ctcgtaagag aagtccagag caacgttttc catcgggttg aactgcagac cagcaccgta
    30661 ggagaaaccg tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt
    30721 accataaccc acacccacta caccgtagat gcttgcccag tcgttaatgc ggtaagccgg
    30781 accagcagtg atgccgtagt actggttttt gttgtagtca ccagagcttg cagtacggct
    30841 tttctcggtg taagtgaaag aaccgatcac acccagcggg ctgttgtctt cttcatagcg
    30901 gtatttcagg ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc tctgtgcgta
    30961 accgccagtt acggtagaag tcgcagctac ggaagtacct gcggtgaaag ccagaactgc
    31021 ggccagtgct gaaagacatg caattttttt cataaccacc tcaaatgtga ttcaaataag
    31081 tcctaagttt taaatatatc aaaaattaat gggaaactct tcgcgatttg tgatgtctaa
    31141 cgggccattt catgtaacag aacgtttcca tacaccgcta tccatctaaa tttaaatcac
    31201 tttttcagag aactgcgtaa gtattacgca tgttttccct gtcattcatc cagattattc
    31261 ctaatcacca gactaatgat tccatcaatc ctggcgcatt ttagtcaaaa cgggggaaaa
    31321 ttttttcaac aaatgctcaa ccagcattgg gtatatccag tacactccac gctttactta
    31381 agtctagata tttgtgggag aaaggatgcc tggttcatta cgtaaaatgc cggtctggtt
    31441 accaatagtc atattgctcg ttgccatggc gtctattcag ggtggagcct cgttagctaa
    31501 gtcacttttt cctctggtga gcgcaccggg tgtcactgcg ctgcgtctgg cattaggaac
    31561 gctgatcctc atcgcgttct ttaagccatg gcgactgcgc tttgccaaag agcaacggtt
    31621 accgctgttg ttttacggcg tttcgctggg tgggatgaat tatctttttt atctttctat
    31681 tcagacagta ccgctgggta ttgcggtggc gctggagttc accggaccac tggcggtggc
    31741 gctgttctct tctcgtcgcc cggtagattt cgtctgggtt gtgctggcgg ttcttggtct
    31801 gtggttcctg ctaccgctgg ggcaagacgt ttcccatgtc gatttaaccg gctgtgcgct
    31861 ggcactgggg gccggggctt gttgggctat ttacatttta agtgggcaac gcgcaggagc
    31921 ggaacatggc cctgcgacgg tagcaattgg ttcgttgatt gcagcgttaa ttttcgtgcc
    31981 aattggagcg cttcaggctg gtgaagcact ctggcactgg tcggttattc cattgggtct
    32041 ggctgtcgtt attctctcga ccgctctgcc ttattcgctg gaaatgattg ccctcacccg
    32101 tttgccaaca cggacatttg gtacgctgat gagcatggaa ccggcgctgg ctgccgtttc
    32161 cgggatgatt ttcctcggag aaacactgac acccatacag ctgctggcgc tcggcgctat
    32221 catcgccgct tcaatggggt ctacgctgac agtacgcaaa gagagcaaaa taaaagaatt
    32281 agacattaat taaatttaca tttctgtatg gttatgcata accatgcaga atttctcgct
    32341 acttttcctc tacaccgtat ttatatatcg aattatgcaa aagcatattt attccgaaaa
    32401 ttcctggcga gcagataaat aagaattgtt cttatcaata tatctaactc attgaatctt
    32461 tattagtttt gtttttcatg cttgttacca ctattagtgt gataggaaca gccagaatag
    32521 cggaacacat agccggtgct atacttagtc tcgttaatta ctgggacata acatcaagag
    32581 gatatgaaat tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata
    32641 cccgcaacga tgtctccgac agcgagaaaa aagcaacagt agagttgctg aatcgccagg
    32701 ttatccagtt tattgatctt tctttgatta ccaaacaagc gcactggaac atgcgcggcg
    32761 ctaacttcat tgccgtacat gaaatgctgg atggcttccg caccgcactg atcgatcatc
    32821 tggataccat ggcagaacgt gccgtgcagc tgggcggtgt agctctgggg accactcaag
    32881 ttatcaacag caaaaccccg ctgaaaagtt acccgctgga catccacaac gttcaggatc
    32941 acctgaaaga actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg
    33001 gcgaagcgaa agatgacgac accgcagata tcctgaccgc cgcgtctcgc gacctggata
    33061 aattcctgtg gtttatcgag tctaacatcg aataaatcca tcgctgatgg tgcagaactt
    33121 tagtacccga taaaagcggc ttcctgacag gaggccgttt tgttttgcag cccacctcaa
    33181 cgcacttatt tagtgcatcc atctgctatc cccagctgat taagtaaatt ttttgtatcc
    33241 acatcatcac acaatcgtta cataaagatt gttttttcat caggttttac gctaaataat
    33301 cactgtgttg agtgcacaat tttagcgcac cagattggtg ccccagaatg gtgcatcttc
    33361 agggtattgc cctataaatc gtgcatcacg tttttgccgc atctcgaaaa atcaatgagt
    33421 tgcaaaattg gcacgatttt ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga
    33481 tagaaaaaag gaaatgctat gaagtctgta ttaaaagttt cactggctgc actgaccctg
    33541 gcttttgcgg tttcttctca tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc
    33601 ttcgttccgt ttgaatttaa acagggcgat aaatatgtgg gctttgacgt tgatctgtgg
    33661 gctgccatcg ctaaagagct gaagctggat tacgaactga agccgatgga tttcagtggg
    33721 atcattccgg cactgcaaac caaaaacgtc gatctggcgc tggcgggcat taccatcacc
    33781 gacgagcgta aaaaagcgat cgatttctct gacggctact acaaaagcgg cctgttagtg
    33841 atggtgaaag ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt
    33901 gctgtgaaga gcggtactgg ctccgttgat tacgcgaaag caaacatcaa aactaaagat
    33961 ctgcgtcagt tcccgaacat cgataacgcc tatatggaac tgggcaccaa ccgcgcagac
    34021 gccgttctgc acgatacgcc aaacattctg tacttcatca aaaccgccgg taacggtcag
    34081 ttcaaagcgg taggtgactc tctggaagcg cagcaatacg gtattgcgtt cccgaaaggt
    34141 agcgacgagc tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact
    34201 tacaacgaaa tctacaaaaa atggttcagt actgaaccga aataataacg ctacacctgt
    34261 aaagcgcact ggcagttccc tctcccccct ggggagagga ttagggtgag gggcgcaaac
    34321 ccgctccggg gccattaatt accctgaatt tgattattta caccacggta acaggaacaa
    34381 catatgcagt ttgactggag tgccatctgg cctgccattc cgcttctgat tgaaggtgcc
    34441 aaaatgaccc tgtggatttc ggtcctcggt ctggctggcg gtctggtaat cggattgctg
    34501 gcaggttttg cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt
    34561 gaagtgatcc gcggcacacc tatcgtcgtc caggtgatgt ttatctattt cgccctgccg
    34621 atggcgttta acgacttacg catcgaccca tttactgcgg cggtggtcac catcatgatc
    34681 aactccggcg cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat ccacaaaggt
    34741 tttcgtgaag caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt
    34801 ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt
    34861 aaagacacct cgctgtttat tgttatcggc gtggcggaac tgacccgtca ggggcaagaa
    34921 attattgccg gtaacttccg cgcccttgag atctggagcg ccgtggcggt gttctatctg
    34981 attattaccc tggtgctgag ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg
    35041 tgattgaatt taaaaacgtc tccaagcact ttggcccaac ccaggtgctg cacaatatcg
    35101 atttgaacat tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat
    35161 cgaccctgct gcgctgcatc aacaaactgg aagaaatcac ctccggcgat ctgattgtcg
    35221 atggcctgaa ggtcaacgat ccgaaagttg acgagcgcct gattcgccag gaagcaggta
    35281 tggtgttcca gcagttttac ctcttcccgc atctgacggc gctgggaaac gtcatgtttg
    35341 gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg cgtgagctgc
    35401 tggcgaaagt cggtctggca gaacgtgcac atcactaccc ttccgaactt tctggtggtc
    35461 aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg atgctgtttg
    35521 atgaaccgac ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg
    35581 atctggctga agaagggatg acgatggtga tcgtgaccca cgaaatcggt tttgccgaga
    35641 aagtagcttc gcggctgatc tttatcgaca aaggccggat tgcggaagat ggcaatccgc
    35701 aggtgttgat caagaacccg ccgagccagc gcttgcagga atttttgcag cacgtctctt
    35761 aataagacac attgcctgat gcgctacgct tatcaggcct acaggatatc tggcaactta
    35821 ttaaaattgc atgaacttgt aggacggata aggcgttcac gccgcatccg gcaaaaaagc
    35881 ccgcacgttg tcagcaacct gcttaatatc ccttcctccc tttcacccga aagggaggca
    35941 caccagattc ctctcattta aaatcgcccc tcccccagca tctatactta tctttttgct
    36001 ctgttttctc actggaggag tcatgcggtg gatcctgttc atcctcttct gcctgctggg
    36061 cgcacctgcc cacgcggtat ccatacccgg cgttacaacc acaacgacaa cggactcaac
    36121 gactgaaccg gccccggaac cggatatcga acaaaaaaag cggcctatgg cgcactggcg
    36181 gatgtgctgg ataacgacac ctcgcgtaaa gagttgatcg accagttgcg tactgtcgcc
    36241 gcgacgcccc ctgcggaacc ggtcccgaag atcgtgccgc cgacgctggt tgaagagcaa
    36301 accgtgctgc aaaaggtcac cgaagtcagc cgccattatg gtgaagccct ttccgcccgc
    36361 ttcgggcaac tttatcgcaa tatcaccggc tccccgcata agccatttaa tccacaaacc
    36421 ttcagcaatg cgctgagcca tttttcaatg ttagcggtat tagtgtttgg tttttactgg
    36481 ctgattcgcc tgtgcgcact gccgctatat cgcaaaatgg gtcagtgggc gaggcaaaaa
    36541 aatcgtgagc gcagcaactg gttgcagctt ccggcgatga ttattggggc gtttattatc
    36601 gacctgctgt tactggccct gacattattt gtcggccagg tattaagcga caacctgaat
    36661 gcaggcagtc gcaccatcgc tttccaacaa agtttgtttc tcaacgcctt tgccctgatt
    36721 gaatttttca aagccgtact acgcctgatt ttttgcccaa acgtggcgga gctgcgcccg
    36781 ttcacgattc aggacgagag cgcccgttac tggggtcgtc gcctgagctg gttaagcagc
    36841 ctgattggct atggcctgat tgtggccgtg ccgattatct ctaatcaggt gaatgtacag
    36901 ataggtgcgc tggcgaacgt catcattatg ctgtgcatga ccgtctgggc gttgtacctg
    36961 atcttccgaa ataaaaaaga gattacccag catatgctca actttgcgga gcattcgctg
    37021 gcttttttca gcctgtttat ccgcgccttt gcgctggtgt ggcactggct ggcaagcgcc
    37081 tattttatca tgctgttttt cttttcgttg ttcgatccgg gcaacagtct gaaatttatg
    37141 atgggtgcaa cggtgcgcag cctggcgatt attggtatcg cagcgtttgt ttccggtatg
    37201 ttttcccgct ggctggcgaa aaccatcacc ctctcgccac atactcagcg taactatccg
    37261 gagctacaaa aacggctgaa tggctggctt tcggcggcgc tgaaaacggc gcgtattctg
    37321 accgtctgcg tggcagtaat gttgctgttg agcgcatggg gattgttcga tttctggaac
    37381 tggctgcaaa acggcgcggg gcagaaaacc gtagatatcc tgatccgcat cgcactcatt
    37441 cttttcttct cggcggttgg ctggacggtg ctcgccagtt tgatcgaaaa ccggctggct
    37501 tcggatattc atggccgccc gctacccagc gcccgtacgc gtaccctgct gacgctgttc
    37561 cgtaacgcgc tggcggtgat tatcagtacc atcaccatca tgattgtgtt gtcggaaatc
    37621 ggcgtcaata tcgcgccatt gctggcaggt gccggggcat taggtctggc tatctcgttt
    37681 ggttcgcaaa cgctggtaaa agatattatc accggggtat ttattcagtt tgaaaacggc
    37741 atgaacactg gagatttggt gactatcggg ccgttgaccg gcacagtgga acggatgtcg
    37801 attcgctccg tgggcgtgcg ccaggatacc ggggcgtatc acatcattcc gtggtcttcg
    37861 ataaccacct ttgctaactt cgtccgcggt attggttcgg tagtggcaaa ctacgatgtt
    37921 gatcgccatg aagatgctga taaagccaat caggcactga aagatgcggt aacggaattg
    37981 atggaaaacg aagaaattcg cgggctgatt attggtgaac cgaactttgc cgggattgtc
    38041 ggcttaagca ataccgcgtt tacactgcgt gtttcgttca ccacgctgcc actcaaacag
    38101 tggacggtac gctttgccct cgacagccag gtgaaaaaac atttcgacct ggcgggcgtt
    38161 cgcgcgccag tgcagactta tcaggtgctg cctgctccgg gcgcgacccc ggctgaaccg
    38221 ctgccgccgg gggagccaac gctttaacgt tggcgattaa caaaacggcg gcgctgctcg
    38281 tcgttcataa aggtccaggc aataaagcga ctctgctttt gcccctgggc catctctttt
    38341 tttaccacct tcaccgcgcc cacgtcggtc agagcacgat acaacggcgg taagttttca
    38401 ccacgagata ccagcgaggt aaaccacatc acctgcttcg cgaagccttt gctctcttca
    38461 atcatctttt tgataaagtt gacttcaccg ccttcacacc acaactcctg ttgctgacca
    38521 ccaaagttca gtgcatcgtc tttgttcagc cccaggttac ggcgtttacg ctcactacct
    38581 gcccgcgccg cagcggcgga atcgtggaat ggcgggttac acaaggtcgc gtcgtattgc
    38641 tcgtttttgt ggatgatgcc gttaaaaatc gccccactct ctttttgccg acgcagacga
    38701 atggcgcggt taagccccgg attagcactg ataatcgcct gcgcactgct taacgcctgg
    38761 ctgctggttt cactaccggt aaaacgccag ccatattcat gtacgccaat cagcggataa
    38821 atacagttcg caccaacgcc gatatccaga atgctggcat tcgctggaat tgttccgctt
    38881 gcttcagcca gtaaatcggc aaggtgatga atataatccg cccggcccgg tactggtggg
    38941 cagagaaaac cgtcggggat atcccagttc gctacggcgt aaaaatgggc cagcaacgcc
    39001 ttattgagcg ccttcaccgc cagcggattg gcaaagtcta cgctttgctc ccctgcgggt
    39061 gtaagcgtga ggaattgcct gagttcagga ttgacctgac aaagcgtggc gagatcgtag
    39121 cggctgtgat gacggttgcg cggatgcaac cccggtttct gggcggacat agcattctcc
    39181 tttaagcagc ggcgtaagat acccgcataa cgcgacaggg taaatatctg gctgcgttta
    39241 tcattcccca aaaccttaac atggctatgg tttatggcaa ttcatcataa aaatcagcat
    39301 tatgcatgtc attgatttgt cttattttgt aaaaatatat cgttcaaaaa ataagcattt
    39361 ctctgtcaat tttttgcgtc atttgcatcg cgcaggcgac ctacacttat caggcactac
    39421 ccgttgtatt tatgaggatg tcattatgaa aaagtgcctc acactactga ttgccaccgt
    39481 cctgagcgga atctctctca cggcttatgc cgcgcaaccg atgagtaacc tggacagcgg
    39541 tcagctacgg cccgccggta ccgtttcggc aactggcgca tcaaacctaa gcgatctgga
    39601 ggataaactg gcagaaaaag cgcgcgaaca aggcgcgaaa ggttatgtca ttaattccgc
    39661 aggcggaaat gaccagatgt taggtactgc aaccatctac aaataacccc accaacgtaa
    39721 ctgcattcgc ctctggatgc agttttcgcc tgttttgtaa tcaaaaaaca tcaaatatga
    39781 acgcaatgta aataaatgta tttctttttc gcgcaatggg tgatagaaaa tcgcatcaag
    39841 tgataatgct tatcaaaatt attatcactt gcacgaacac tatcacggga ttaacagtgg
    39901 catcgcatcc gcagagatgc tttctcgtgg cagtgaaaat ttcaacatat aagaaaaagt
    39961 cacctgcaaa atggaaaaca atcgcaattt ccctgccaga caatttcatt cgctcacgtt
    40021 ctttgccggt ctttgtattg gtattacgcc tgtggcacag gcactcgccg ccgaagggca
    40081 agctaacgcg gatgacacgc tggttgtcga agcatcaacg ccttcgcttt atgcgccaca
    40141 acaatctgcc gatccgaaat tctcgcgtcc ggtagcggat actacccgca cgatgacggt
    40201 gatttctgaa caagtgatta aagatcaggg cgcaaccaac cttaccgacg cgctcaaaaa
    40261 c
//
LOCUS       DAJTYH010000025        40245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTYH010000025 DAJTYH010000000
VERSION     DAJTYH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            149..1582
                     /gene="cscA"
                     /locus_tag="OO609_001314"
     CDS             149..1582
                     /gene="cscA"
                     /locus_tag="OO609_001314"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6632799.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1590..2648)
                     /locus_tag="OO609_001315"
     CDS             complement(1590..2648)
                     /locus_tag="OO609_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6632800.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2816..3034
                     /locus_tag="OO609_001316"
                     /pseudo
     CDS             2816..3034
                     /locus_tag="OO609_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3052..4380
                     /gene="dsdA"
                     /locus_tag="OO609_001317"
     CDS             3052..4380
                     /gene="dsdA"
                     /locus_tag="OO609_001317"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6632801.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4488..6026)
                     /gene="emrY"
                     /locus_tag="OO609_001318"
     CDS             complement(4488..6026)
                     /gene="emrY"
                     /locus_tag="OO609_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6632802.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6026..7189)
                     /gene="emrK"
                     /locus_tag="OO609_001319"
     CDS             complement(6026..7189)
                     /gene="emrK"
                     /locus_tag="OO609_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6632803.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7605..8219
                     /gene="evgA"
                     /locus_tag="OO609_001320"
     CDS             7605..8219
                     /gene="evgA"
                     /locus_tag="OO609_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6632804.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8224..11817
                     /gene="evgS"
                     /locus_tag="OO609_001321"
     CDS             8224..11817
                     /gene="evgS"
                     /locus_tag="OO609_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6632805.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11873..13018)
                     /gene="yfdE"
                     /locus_tag="OO609_001322"
     CDS             complement(11873..13018)
                     /gene="yfdE"
                     /locus_tag="OO609_001322"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6632806.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13092..14036)
                     /gene="yfdV"
                     /locus_tag="OO609_001323"
     CDS             complement(13092..14036)
                     /gene="yfdV"
                     /locus_tag="OO609_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6632807.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14106..15800)
                     /gene="oxc"
                     /locus_tag="OO609_001324"
     CDS             complement(14106..15800)
                     /gene="oxc"
                     /locus_tag="OO609_001324"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6632808.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15854..17104)
                     /gene="frc"
                     /locus_tag="OO609_001325"
     CDS             complement(15854..17104)
                     /gene="frc"
                     /locus_tag="OO609_001325"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6632809.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17617..18240)
                     /locus_tag="OO609_001326"
     CDS             complement(17617..18240)
                     /locus_tag="OO609_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6632810.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18537..18812
                     /locus_tag="OO609_001327"
     CDS             18537..18812
                     /locus_tag="OO609_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632811.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18889..19131)
                     /locus_tag="OO609_001328"
     CDS             complement(18889..19131)
                     /locus_tag="OO609_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6632812.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19484..20404
                     /gene="lpxP"
                     /locus_tag="OO609_001329"
     CDS             19484..20404
                     /gene="lpxP"
                     /locus_tag="OO609_001329"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6632813.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20759..20830
                     /gene="ypdK"
                     /locus_tag="OO609_001330"
     CDS             20759..20830
                     /gene="ypdK"
                     /locus_tag="OO609_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6632814.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20895..22133)
                     /gene="alaC"
                     /locus_tag="OO609_001331"
     CDS             complement(20895..22133)
                     /gene="alaC"
                     /locus_tag="OO609_001331"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6632815.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22510..23996
                     /locus_tag="OO609_001332"
                     /pseudo
     CDS             22510..23996
                     /locus_tag="OO609_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24011..24745
                     /gene="ypdB"
                     /locus_tag="OO609_001333"
     CDS             24011..24745
                     /gene="ypdB"
                     /locus_tag="OO609_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6632816.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24758..25615
                     /locus_tag="OO609_001334"
                     /pseudo
     CDS             24758..25615
                     /locus_tag="OO609_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25618..28113)
                     /gene="ptsP"
                     /locus_tag="OO609_001335"
     CDS             complement(25618..28113)
                     /gene="ptsP"
                     /locus_tag="OO609_001335"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6632817.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28138..29175)
                     /gene="ypdE"
                     /locus_tag="OO609_001336"
     CDS             complement(28138..29175)
                     /gene="ypdE"
                     /locus_tag="OO609_001336"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6632818.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29175..30258)
                     /gene="ypdF"
                     /locus_tag="OO609_001337"
                     /pseudo
     CDS             complement(29175..30258)
                     /gene="ypdF"
                     /locus_tag="OO609_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30273..31520)
                     /locus_tag="OO609_001338"
     CDS             complement(30273..31520)
                     /locus_tag="OO609_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6632819.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31542..31868)
                     /locus_tag="OO609_001339"
     CDS             complement(31542..31868)
                     /locus_tag="OO609_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6632820.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32087..33052)
                     /gene="glk"
                     /locus_tag="OO609_001340"
     CDS             complement(32087..33052)
                     /gene="glk"
                     /locus_tag="OO609_001340"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6632821.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33256..34512
                     /locus_tag="OO609_001341"
     CDS             33256..34512
                     /locus_tag="OO609_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6632822.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34627..34953
                     /locus_tag="OO609_001342"
     CDS             34627..34953
                     /locus_tag="OO609_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6632823.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35094..36332)
                     /locus_tag="OO609_001343"
     CDS             complement(35094..36332)
                     /locus_tag="OO609_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6632824.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36668..37870
                     /gene="nupC"
                     /locus_tag="OO609_001344"
     CDS             36668..37870
                     /gene="nupC"
                     /locus_tag="OO609_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6632825.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37920..40032)
                     /locus_tag="OO609_001345"
                     /pseudo
     CDS             complement(37920..40032)
                     /locus_tag="OO609_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT        10739 a        10006 c         9300 g        10200 t
ORIGIN      
        1 tttactaaaa catgcatatt cgatcacaaa acgccatagt taacgttaac atttgcgata
       61 ttcatcgcat ttatgaaagt aagggactat atttttataa aagttaacgt taataattca
      121 ccaaatttgc ttaaccagga tgattaaaat gacgcaatct cgattgcatg cggcgcaaaa
      181 cgcactagca aaacttcatg agcaccgggg taacactttc tatccccatt ttcacctcgc
      241 gcctcctgcc ggatggatga acgatccaaa cggcctgatc tggtttaacg atcgttatca
      301 cgcgttttat caacatcacc cgatgagcga acactgggga ccaatgcact ggggacatgc
      361 caccagtgac gatatgatcc actggcagca tgagcctatt gcgctagcgc taggagacga
      421 taatgacaaa gacgggtgtt tttcaggtag tgctgtcgat gacaatggtg tcctctcact
      481 tatctacacc ggacacgtct ggctcgatgg tgcaggtaat gacgatgcaa ttcgcgaagt
      541 acaatgtctg gctaccagtc gggatggtat tcatttcgag aaacagggtg tgatcctcac
      601 tccaccagaa ggaatcatgc acttccgcga tcctaaagtg tggcgtgaag ccgacacatg
      661 gtggatggta gtcggggcga aagatccagg caacacgggg cagatcctgc tttatcgcgg
      721 cagttcattg cgtgaatggg ccttcgatcg cgtactggcc cacgctgatg cgggtgaaag
      781 ctatatgtgg gaatgtccgg actttttcag ccttggcgat cagcattatc tgatgttttc
      841 cccgcaggga atgaatgccg agggatacag ttaccgaaat cgctttcaaa gtggcgtaat
      901 acccggaatg tggtcgccag gacgactttt tgcacaatcc gggcatttta ctgaacttga
      961 taacgggcat gacttttatg caccacaaag ctttttagcg aaggatggtc ggcgtattgt
     1021 tatcggctgg atggatatgt gggaatcgcc aatgccctca aaacgtgaag gatgggcagg
     1081 ctgcatgacg ctggcgcgcg agctatcaga gagcaatggc aaacttctac aacgcccggt
     1141 tcacgaagct gagtcgttac gccagcagca tcaatctgtc tctccccgca gaatcagcaa
     1201 taaatatgtt ttgcaggaaa acgcgcaagc agttgagatt cagttgcagt gggcgctgaa
     1261 gaacagtgat gccgaacatt acggattaca gctcggcact ggaatgcggc tgtatattga
     1321 taaccaatct gagcgactta ttttgtggcg gtattaccca cacgagaatt tagacggcta
     1381 ccgtagtatt cccctcccgc agcgtgacac gctcgcccta aggatattta tcgatacatc
     1441 atccgtggaa gtatttatta acgacgggga agcggtgatg agtagtcgaa tctatccgca
     1501 gccagaagaa cgggaactgt cgctttatgc ctcccacgga gtggctgtgc tgcaacatgg
     1561 agcactctgg ctactgggtt aacataatat caggtggaac aacggatcaa cagcgggcaa
     1621 gggatccgcg tcactcttcc cccttcacga ccttcaataa tatgcaatgc agcttcccgc
     1681 ccgataatgt catgtggaag ctgaattgtg gtcagcggcg gtaaaaacag atgcccgacg
     1741 ccaaccagat tatcaaagcc cattacagcg acatcctgcg ggatacgtac ccccttcgcc
     1801 aaaagaacct gataagccac aaaggctgcg cgatcgttac cacatatcag aacatcaaaa
     1861 tctggtttgc ccgatttgaa gtgagcattg agtaaacttg cgagatcggt gtagtgatca
     1921 tcacctgttg ccatgtgaaa ttgtttcacc tcagccagat ctcgtccagc atcacgccaa
     1981 gcctgctcaa atccctgccg acgataccct gttgccaacg cactttccgg tagccagaag
     2041 cataatggtt gacgatagcc cgccgcgagc aaatgctgtg ttgattcata ttgtgcagtg
     2101 taatcatcag ggatataact gggtaacgct gggtcatccg ccacacagtt cgccaataca
     2161 atattttcac catacagaga ctcaggcagc gtgatatgtc gcagccccat tgtagtatag
     2221 ataatgccat ccggacggtg ggcaagcagc tgacgtgccg cgcgggcagc gtcatcttca
     2281 gaaaaaatat tgattaaaaa actattccag ccgaactcgc tggcggtttg ctcaatggca
     2341 agcagaatat caacagagaa aggagtggta gccgtgtcct gcgccagcac ggcgagagtc
     2401 gacggcttac gtccttgagc gcgcatctta cgggcggaaa gatcaggaac ataattcagg
     2461 gtctggattg cctgcaatac gcggtcacgc gttgcaggac gcacagattc tgcattatgc
     2521 atcacccggg agactgtcat catcgacact cccgccaggc gtgcgacatc ctttaatgaa
     2581 gccataccca agccgtttgc cgtaaaacgg gcactgtagc agaaacagac gtcactggcg
     2641 agatccaacg ccctatcacc tgacacagca atacaataaa aaataacaat aattcccgga
     2701 caattgtccc caattccgcc tctgttctcg ccaacgagta accatatagg caagtaagta
     2761 aaacatgcca actggcatcg ccaacctgga gaagaaaata taaatatgga tcattgtgct
     2821 gccactgtat cccgacatca gcccggaaat tattgcgatt gctatcggtt caggtgcaat
     2881 tggctgcacg atcgttacgg actcgctttt ctggctggtg aagcaatatt gcggcgctac
     2941 gctcaatgaa acatttaaat actatacgac agcgacattt atcgcttcag tcgtcgctct
     3001 ggcgggcaca ttcctgctgt catttatcat ctaagcgcaa agagacgtac tatggaaaac
     3061 gctaaaatga actcgctcat cgcccagtat ccgttggtaa aggatctggt tgctcttaaa
     3121 gaaaccacct ggtttaatcc tagcacgacc tcattggctg aaggtttacc ttatgttggc
     3181 ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc accctatctg
     3241 gcaaaagcat ttgctgaaac agctgccact ggggggatta ttgaatcaga actggttgcc
     3301 attccggcta tgcaaaaacg gctggaaaag gaatatcaac aaccgatcag cgggcaactc
     3361 ctactgaaaa aagatagcca tttgcccatt tccggctcta taaaagcacg cggcgggatt
     3421 tatgaagtcc tggcacatgc agaaaaactg gctctggaag cggggttgct gacgcttgat
     3481 gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca atacagcatt
     3541 gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc ccgcattggc
     3601 tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc gaaactgcgc
     3661 agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt cgaggaagga
     3721 cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa ttcccgcacg
     3781 ttgttccttg ggtattccgt tgctggccag cgtcttaaag cgcaatttgc ccagcaaggc
     3841 cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt tggcggtggt
     3901 cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca ctgctttttt
     3961 gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt acacgatcag
     4021 atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct tgcagttggt
     4081 cgcgcatcat gctttgtcgg gcgggcaatg gagcgtctgc tggatggctt ctataccctt
     4141 agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg tattcgtctt
     4201 gaaccttcag cactggcggg tatggccgga cctcagcgcg tgtgtgcatc agtaagttac
     4261 caacagatgc acggtttcag cgcagaacaa ttgcgtaatg ccactcatct ggtgtgggcg
     4321 acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa aggccgttaa
     4381 taacgtttca acgcaacatc gcaatccttt ccctgggtga gcgatgctgc cgatggcgca
     4441 gacttaagat ccccggtctt acccgctata accccctcct tttatcatca cccaacgcct
     4501 ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat aaaagcaata
     4561 gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt tatttcatta
     4621 ttaatttcat taagaactcc tgaaagcgaa ccatagtatt tatccataat ttgtgacgat
     4681 gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg gtgtaacgat
     4741 tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga caaggtacga
     4801 aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc tgaaaacgaa
     4861 atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctggaaaaa ctgtggcaaa
     4921 atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta atagcaaacc
     4981 gcatacatca aaaaactgaa tgtcactaac acccgcatgt ctattttatt gccataacgt
     5041 cctatcaaag gtgaaatcaa tagtggcatg atgccgatgg gcgcataagc aagcccggcc
     5101 catatcgcat tataccccat cgttttctgg agtaactgcg gcataaggac gatcgctcca
     5161 gagtaaaata aatacgcgca ggtgatactc acaataccaa tggtgaaatt acgggactta
     5221 aacaaactga gatcaagaat cgggttctct gaggtcgatt cccaaatgac taaagagatc
     5281 agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa ccaatccaga
     5341 tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa cagggtcagt
     5401 cttggtagat tcatttttac cggtgaagtt tcagtttctc ttcctttaag taaggttaag
     5461 cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca accccagcta
     5521 aagttatcgc aaatatagcc gcccaatatc ggcccacata tcggagcgat aatcacggtc
     5581 attgaccata atgccagagc aaatgttctt ttttctggtg gataattcct taataataaa
     5641 ctctgtgaca gtggaattaa cggccccgcc attaatccct gaacgactct aaaaaatatc
     5701 agcacatcaa gattggtcga taaactacac attaaggaag acagcgaaaa aaaagtgact
     5761 gaaagtaaaa ataaccttaa ttcgcctatt ctttgtgcca gcctgccggt aacagggatc
     5821 gcaatggcat ttgctacgcc aaacgaggtg ataacccagg tgccttcgtc tgttgatgct
     5881 cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc caacatttgc
     5941 ataaacgtcg ctaatgacaa tgcaatagta acgcaccata acgtcccacc ggttaatggt
     6001 gccggagttg atttagtgat tgccattaaa gttgtccatt atgcgaaata atgttgctaa
     6061 tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa gcaggcatgg
     6121 aggtcacggt tgaagccaga tcaggcatct cagcaatgtc ttcgtccttc gtatcaatag
     6181 ttgcagtcat cgataaacca atgcgcaagg ggtgttccat gagttctttt ggatcaagag
     6241 aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca ttttgtgcag
     6301 gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga ccatgaaaca
     6361 caacattttc accataaaga tcgctgataa tattgactga ttgaccaatc cgtacatccg
     6421 tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt acgacagcca
     6481 ttaacgattg tccggaactc actgtttcgc cgacctgaac acttcgctgg gcaatatagc
     6541 cagtaaccgg actccttata tccgtacgtt taagcaccaa ccaggcttct ttggttgcat
     6601 ccgccgcttc aactacttgt ggctgacggt ttaatggtgt gttcattact aaagctttat
     6661 tcgctttata agcctggata gcggcattca gtgccgcttt gctacttatt aacgtatctt
     6721 tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaaaggc acacgacggt
     6781 tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg gcactgtatt
     6841 gtttatcctg taagtacagt ttattcgttt gccgaacaat atttgccaga ttatttttag
     6901 ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg tcaccctgtc
     6961 gaacgtagtt cgtatcttta tgattaacta cagtgacact acctgagact tgtgcagaaa
     7021 ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct tctaattcca
     7081 ttgaccaata ggcataggca cctgaaaacg caataaataa aactatcacc aataaagaaa
     7141 agtatttttt tctgttagac tgttttttat ttgaattaat ctgttccact attatctctc
     7201 atttcgcata gatgaaattt atgaattgaa tgaaaagagt attcattctt catatcaaac
     7261 acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa atgaggtgca
     7321 aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg cctgtaggat
     7381 tagtgagaag acttatagtg ctaacttgaa actataaatc atcggtataa tccctgattt
     7441 tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga attatcttaa
     7501 aggaaactca gattttctta tttttattga gaaaatgaga tgacgcctta tgtctgtatt
     7561 actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac gcaataatta
     7621 ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa aacgatattg
     7681 aaatcttagc ggagttaact gaaggcggaa gcgccgttca gcgggtggaa acacttaagc
     7741 ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag gtgttagaaa
     7801 cgctgaggaa gcgccaatat agcggaatta ttattatcgt ctccgctaaa aatgaccatt
     7861 tttacgggaa acattgtgct gatgctggcg ctaatggttt cgtgagtaaa aaagaaggca
     7921 tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat ttccccttct
     7981 ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac tccttatcga
     8041 aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat gacattgcgg
     8101 aaaaaatgtt catcagcaac aaaactgtca gcacgtataa aagtcgcctg atggaaaaat
     8161 tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa atcggctaat
     8221 cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc gaccataagt
     8281 ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg tgtcacactt
     8341 gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa aaatcttgtg
     8401 attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca gcaacaggtt
     8461 cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat caaattaaca
     8521 ctccgggaat acgcagatca tcaaaaagca atggacgcgc ttgcagaagg tgaagtcgat
     8581 atagtgttat cacatttagt tacttcgccg cctcttaata atgacattgc tgcaaccaaa
     8641 ccattgataa ttacctttcc ggcgctggta accacccttc acgactcaat gcgaccgctt
     8701 acctcaccaa aaccagtaaa tattgctcgg gtagcaaatt accccccaga cgaggtaatt
     8761 catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca ggcattagca
     8821 tccgtctcag ctgggcacaa tgattacttt attggtagta acatcattac cagcagtatg
     8881 atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc gccgcgtcaa
     8941 tataattttt tcttgaccag aaaagaatct gtcattctta atgaagtact caatagattt
     9001 gttgatgctt taacaaatga agttcgctat gaagtatcac aaaattggct tgatacagga
     9061 aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca gtggattaag
     9121 cagcatccca atttaaaggt gctggaaaat ccttactcgc ccccctattc tatgacggat
     9181 gaaaatggct cggttcgggg cgttatgggg gacattctta atattattac cttgcaaaca
     9241 ggtttaaatt tttctccgat caccgtttca cacaatatcc atgctggaac acaacttagc
     9301 cccggaggat gggatataat acctggcgct atttatagtg aagatcgaga aaataatgtt
     9361 ttatttgctg aagccttcat aacaacgcct tacgtttttg tcatgcaaaa agcgcctgac
     9421 agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta tgagctgcat
     9481 tcgcaattaa aagagatgta tccggaggtt gaatggatac aggtcgataa tgccagcgct
     9541 gcatttcaca agattaagga aggtgaactt gatgctctgg tcgcgacaca gctaaattcg
     9601 cgttacatga tcgatcatta ctatcctaat gaactttatc attttcttat tcctggcgtt
     9661 ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa ggatattatt
     9721 aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga aaaatggatt
     9781 aaaatgccca atgtgaccat tgacacatgg gacctatata gcgagcaatt ttatattgtt
     9841 acgacattat ccgttttatt agttggcagt agccttttat ggggattcta cctgttacgc
     9901 tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc attccgaaaa
     9961 gcactctcgg attccttacc gaatccaact tatgttgtaa actggcaagg taatgtcatt
    10021 agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa tgcaatgtta
    10081 ccattagaaa acagtgattc accctttaaa gatgtttttt ctaatgcgca tgaagtcaca
    10141 gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga taatggcatc
    10201 gagaaaagat gcattaatca ctggcataca ttatgcaatc ttcctgcaag tgacaatgca
    10261 gtatatattt gtggttggca agatattact gaaacgcgtg atctaattaa tgcactcgag
    10321 gtagaaaaaa ataaagcgat aaaggctacc gtagcaaaaa gtcagtttct ggcaacgatg
    10381 agtcacgaaa taagaacacc aataagctct attatgggct tcctggaact tctgtcgggt
    10441 tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc caccggacaa
    10501 tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc gggtaactat
    10561 caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg tcactctttc
    10621 ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt tcctgatcat
    10681 tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt actgagtaat
    10741 gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggg tcacattgat
    10801 gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt atcgcaggaa
    10861 gaacaacaac aactgtttaa gcgctacagc caaacaagtg caggtcgtca gcaaacaggt
    10921 tctggtttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg cgatttatca
    10981 ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt agaaattagc
    11041 cagcaagtgg cgactgtcga ggcaaaagca gaacaaccca tcacactacc tgaaaagttg
    11101 agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg ccagctaaat
    11161 ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca caaagtcagt
    11221 atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga tggttttgag
    11281 ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct tacagccaac
    11341 gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg tttgttcaaa
    11401 ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt
    11461 gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc gaatgatcta
    11521 caactgatgc aggagattct catgactttc cagcatgaaa cgcataaaga tctacccgct
    11581 gcgtttcatg cactagaagc tggcgataac agaactttcc atcagtgtat tcatcgcatc
    11641 cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca tcagttagaa
    11701 ataacacctg cttcagatga cagtaagcct gaaattcttc agctgctgaa ctctgttaaa
    11761 gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa tgactaaatt
    11821 gcggctccca caatgttcta atgtgggagc tatttaccag cacatcttac atttatgatg
    11881 agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat
    11941 gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt cctccggctt
    12001 caatcaacat atttctcgcc tgagtttgtg gcaaattaat ggcctcagcc acacttaata
    12061 acggcgcgac gggcacacca acttcatgta ttttggctaa ccaaacttca gctgcctgcg
    12121 tttttaacgt tcgctcaata tattgtttaa gaatagcctg gttttgtacg cgtaaaatat
    12181 tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag
    12241 aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta tcgaaaacat
    12301 caaaaggtgc catgtaggga tggcgatttc ccaggcgttg tggtgacttc cctgtcgcaa
    12361 tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc gctatatcga
    12421 catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt ccgctgaata
    12481 aatagacacc gccgcataga tccgcaagag aggtaccaac acgcactggc ggagcatcag
    12541 ggtaccctgt ttccatcatt atcccactca ttgcctgaat gatggtatcg taggcaggag
    12601 catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg aggcgcgggt
    12661 tgatttcttg taacctttcc catgaaaacc ccagtttttc cattgtacct aggcgaaaat
    12721 tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta tcgtgatcat
    12781 tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa tagagtgact
    12841 gtccatccac atagggacca aatgtgcggg tatcatcacc atgacctggt ggctcaactt
    12901 taattaccct tgcgcccata ttataaagaa gttgagttcc gaaaggtcca ttaaggacat
    12961 gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta tttgtcattt
    13021 gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg taaaatcaat
    13081 aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc cgtgacgaca
    13141 aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac attaaaccgg
    13201 ctggcaataa tgattccgga gaatgccggc ggtaatgcgc ctgccagtac catcatctgc
    13261 aaatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagcgccag tggcatcaga
    13321 atcaacttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt atgtgcagcc
    13381 agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat cagattaaag
    13441 gttgggtccc atgctgccgg aatttttacc ccaaccagca ccaggatcgt tgccagaaca
    13501 ggtgcccata ctactggctc ctttgccgca gaaattaatg cgctcagatt actattcttc
    13561 ttaccatccg ctcctgagga aggattcagc aaatacagac caataggaat agtaattgcg
    13621 ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc accataaata
    13681 ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacccgcaat taatgcacat
    13741 acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccaaacca ggagaaaaag
    13801 aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc aaaaatcatt
    13861 tccctgtttg cccgggtaat agaaacaaat agagccgcag gaagagcgta gtttaatacc
    13921 agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc gctaaaataa
    13981 cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt tagcataata
    14041 gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg agacgatgtc
    14101 agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc actttcagtt
    14161 cctgctgcag ggtcgataac cacattaata atggtcggtt tgcgcgactg aataccggtg
    14221 gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc acgaaacgca
    14281 tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggtgatgg tgcaccagcg
    14341 ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat aacgatcgtc
    14401 accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc aaaagcacta
    14461 tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat ggcatagccc
    14521 ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg tttatacata
    14581 tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa ataaatatcc
    14641 tggttctcgc gcaatacgtc gcgcacagca cttaatgcat taaagtaatt taatggttga
    14701 gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg gatatttaaa
    14761 atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc cagcatacct
    14821 tgcatactgg atgcaatatc accaacgact ggcacagcaa tggggcggtt gctgtcaatt
    14881 tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc ttttttaccg
    14941 tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc atttgccagg
    15001 gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat ccctttcgcc
    15061 atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag ctgttcatca
    15121 gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc ttttgctaaa
    15181 agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga cggattttca
    15241 actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag gacatttgct
    15301 ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat agcgcgtgcc
    15361 aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc atacggtttt
    15421 gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac gatcgcgcgg
    15481 tcgctggagc cgctaatcat gataatcgga aaaccattta ccgttgcgtt ggccaatgcg
    15541 gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg tttttgggta
    15601 agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa accaatataa
    15661 cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc tacaacacca
    15721 taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat accatctgtc
    15781 atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg ggagccagaa
    15841 gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca tcgtcgctat
    15901 aacccagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca gctttaatat
    15961 ccggcgtaaa ggcagagaat ttcattggac aaccaacggt cagatatttt ccacgcaacg
    16021 gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt gaaatttctt
    16081 tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc accgcttcat
    16141 gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa atatgtggct
    16201 gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg ccgatggctt
    16261 tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg ttaggatcgg
    16321 tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct gcattaccac
    16381 cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct tccagataac
    16441 ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc aatacggcat
    16501 cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc agcaaagcag
    16561 caagtaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct gcagcgctta
    16621 ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct gcctgagcaa
    16681 cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct ttgatcgaac
    16741 caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc atgtgatcaa
    16801 tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc ttttccatta
    16861 cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta ctgttaagca
    16921 tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ttggtgacgc gttacgtcac
    16981 caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc atttgagtac
    17041 aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga agtggagttg
    17101 acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat ttaaggcata
    17161 acgccctcta taaataatct gcaaaatatc ttcgtagcgg cactcgatat ttttaaattc
    17221 ctgtatcaag tactgaaaat tattttatcg ccacttccaa ttcctttctg catggtaagt
    17281 agtgaggacg aaaagaagaa tatgataaat attaattcgt ggtaatcaga gagagtcgcg
    17341 acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa gtaagagtat
    17401 gattacgctt tttaaagcgt accataaagc ataacataat aaatgatttg tgatgacacg
    17461 ggattattgc ggtttacttt gaggaaaata aaatgcgtgt ttatagaacc gcgagcgcca
    17521 aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg gctggaggga
    17581 gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca gtgcttcgct
    17641 atcgacgatg attccatctt ccgcaccttt aagcgccagg tttgcctcgt agtattgctt
    17701 tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa gcggcatcag
    17761 atattggttt tccattacgc caacaccggc caggcgaagc tcttccattg cgcccttttt
    17821 atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt cgggtgttgc
    17881 tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt catcattgag
    17941 attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat ctgacagcaa
    18001 agcggaagct tcattggtca gttcttttgc cttttccgga tcgccatgaa acagagccag
    18061 gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac gctctgctgc
    18121 tgccgttttc tgggtttgct gagtttgctg ttgtgccgct accggtgcat tatcagcagc
    18181 ccatacagtt gaagatgcca gaattgctgt gaccatcgta gccataatta aacgtttcat
    18241 acttacctca ttctttaaca gttttagaaa acgccgccat catattgggt gagcatgaaa
    18301 tgagaaatca gagagcaacg gtcattttga aagggttatc ttacaattgt agttattatc
    18361 gcaaagcggc atttagtctt acagttgtag gaatatgcct cctgtcgcag agaactattc
    18421 ttatatacca caaagagatg tcatttatta tattgatgta aatattgatt aattatatgt
    18481 aaatacagcc ataacatcgt atggttctca gtgaaccatt ttcaggataa ctttctatga
    18541 aagtaaactt aatacttttc agcttatttt tattggtctc tattatggca tgcaatgttt
    18601 ttgcattttc catttctggt ggtgtaagtg agaggagcta taaagagact gaaaaaacat
    18661 cagcgatgac gaccacacac tctacaaaac ttcagccatc acaggcgatt ttgtttaaga
    18721 tgagagaaga tgtgccacca ttaaacctca cagaagaaat cacgcctact tatccgacaa
    18781 aggcgaatta tcttattcat cctgtgcgat agcgtctgtc acatggcagg tgaacaatca
    18841 tgtctgatgc tgatgtgaac acctgcccaa atgagccatc ctgacccact atcgcctgcc
    18901 ataccccacc ataaagcgaa tcatcaagcc acaagccggg gcaagaaaca taaatatccc
    18961 cgtcaccagt tcattaccgc cagttaacca catggtgaag taataaatga gtgcagcgag
    19021 gatcaccatg ccgaagaaag aagagaccgc cgataccacg ttcagtacct gaccgatgta
    19081 gccgcttacg cagacaatac acagggcgag aaacatccat aaataaatca tagctcaacc
    19141 tcctgccgcg ctaaaaaacc tgtaacttca gtcactttaa tagtaagaca atccttaacc
    19201 tctggcaatt tttgctaagc atcaatgaaa acagcacgtt aaatgcaacg ctattgcgtg
    19261 cggaaaattt tttactttgc acgattaata atcagtagct gaaagcagtc agccagtgat
    19321 aaagtagagc caccgcattc atgctcctcg tggttatgtc ctgactagtc tttacactct
    19381 ttacaggaac cattgtcgta catgatggcc caaccaattg agtgtaccgc tgtcgacatg
    19441 gaatcttctg cgatacaaca attcgtatct acagaaggta actatgtttc cacaatgcaa
    19501 attttcccgc gagtttctac atcctcgcta ctggctcaca tggtttgggc ttggtgtact
    19561 ctggctttgg gtacagcttc cttatcctgt tctctgcttt ctcggcacgc gtattggcgc
    19621 aatggcgcga ccattcctga aacgtcgtga atctatcgcc cgtaaaaacc tggaactttg
    19681 tttcccgcag cattctgcgg aagaacgcga gaagatgatt gccgaaaact ttcgttcact
    19741 cggcatggcg ctggtagaaa ccggcatggc atggttctgg cccgacagtc gcgtacgtaa
    19801 atggtttgat gttgaagggt tggataacct taaacgcgca caaatgcaaa atcgcggcgt
    19861 aatggttgtc ggcgtccatt ttatgtcgct ggaactgggc ggccgcgtga tgggactgtg
    19921 ccaaccaatg atggctacct atcgtccaca taataatcag ctgatggaat gggtgcagac
    19981 ccgtgggcgc atgcgctcta acaaagcgat gatcggcaga aataatctgc gcggcattgt
    20041 cggtgcactg aagaaaggtg aagcggtatg gtttgctccc gatcaggatt atggtcgtaa
    20101 aggcagctcc ttcgcgccgt tctttgcggt ggaaaatgtc gccacaacca atggcaccta
    20161 tgttctctcc cgtctctctg gcgcagccat gttgaccgta acgatggtaa gaaaagcgga
    20221 ttacagcgga tatcgtttgt tcatcacccc agagatggaa ggctacccga cagatgaaaa
    20281 tcaagccgct gcttatatga acaagattat cgagaaagag atcatgcgcg caccggagca
    20341 gtacctctgg atccaccgtc gctttaaaac gcgcccggtg ggagaatcgt cgttgtacat
    20401 ttaacaaact gctcttactc tttccctcct caaatcggcc ataataacct catgattttt
    20461 atagcttttt tcttaagttt tttccttcgc tgaatatcta attattaccg tcagaaatta
    20521 aactgtcgcc gatatgagac acttgtaagt tactaaaagt aaataagaaa atgcctcttg
    20581 tctcgtttca gttttaggcg tacattagcg ccgtctcgag cagtttagct cagaattctg
    20641 gggtgatgcc cacggtaaaa tacagggctt ttaaggtcgc tttgcccggt gtcaactcac
    20701 tgtatcaggt gtaatgaagt cattcaggcg taacagtaat tacgcggaga gatgtaaagt
    20761 gaaatatttc tttatgggca tttcttttat ggtcatcgtt tgggccggta cttttgccct
    20821 gatgatctaa aagcaaactg tgcaatgaaa aacaggagcc atcaggctcc tgtttgcatt
    20881 tctgacgacg gtgtttattc cgcgttttcg tgaatatgtt tgctgctgac gggtagtaaa
    20941 ccgtcggcac ggaacatcgc tttaatccct ctgatggcct gacgaatacg gtcgtggttt
    21001 tcaatcagtg caaagcgaac atgcgtatca ccatagtcgc caaagccaat ccccggcgag
    21061 acacaaacct tcgcttcgtt aaggagcttc ttggcaaatt ccagcgatcc catggccgca
    21121 tatggttccg ggattttcgc ccagacatac atcgaagcct tcggcatttc gaccatccag
    21181 cccgcttcat gcagcccttt aaccaataca tcacgacggc gtttgtactg ttcggcaatg
    21241 tcgcgcacgc actgttgatc gccctcgagt gccgcaatcg ccgccacctg caacggcgta
    21301 aaagtgccgt aatcgtgata gcttttgatt cgcgccaggg cactgaccag cgttttgttg
    21361 ccgaccataa agccgatacg ccagcccgcc atgttgtagc ttttcgacag cgtaaagaac
    21421 tcgaccgcca catcgcgtgc acccggcacc tgcataattg acggcgcttt ccagccatcg
    21481 tagacgatat cggcataggc caggtcatgg accaccagca catcgtagca tttcgccagc
    21541 gccacaacct tttcgaagaa ctccagttcc acgcattgcg cggttgggtt cgacgggaag
    21601 ccgaggatca tcatcttcgg tttcggataa ctttcacgaa tggcgcgctc cagttcgttg
    21661 aagaaatcga caccttccac cagcggcact gagcgtacct gcgccccggc aatcaccgcg
    21721 ccataaatat ggatcgggta acttggattc ggcaccagca ccgtgtcacc atgatccagc
    21781 gtcgccagca tcagatgcgc caggccctct ttcgaaccaa tagtgacgat ggcttctgat
    21841 tccgggtcga tttcaacgtc gtagcgatcc tgataccagc gggaaatggc gcgacgtaac
    21901 cgcggaatgc cgcgtgaagt ggagtaacca tgcgtgtccg ggcgctgggc cacagtacat
    21961 aatttttcga cgatatgcgg cggagtcgca ccgtccgggt tacccatgct gaaatcgata
    22021 atatcttcgc cgcgccgacg cgcagccatt ttcagttcag cggtgatgtt aaaaacgtag
    22081 ggcgggagac gatcaatgcg cgtaaagcga cgttcagggc gagtgtcagc cataatttcc
    22141 tcagattaac gttagcgccc ggaccgtccg agcgacgctg ccacaatggt ggctgttttg
    22201 aaaatagcct gattaatttc tgtctgtcca gcgttttaga acatttttat caatggattt
    22261 aaatgaggaa ctccacttca aaaacaggaa atttttctac gctaacaaga aaatcaccac
    22321 caattttatt aacattataa tttcaataaa tttaccagcc agaagtgcat tatcaagaga
    22381 tacattgcta atacaccatt tatcttttaa tcccctttta aatttatggt ttttccttaa
    22441 aaacggcctg ctggtcatca gccttcaggt tacctatcat agaggtttaa tccttattca
    22501 gagtcacccg tgcacgaaat attcaacatg ctgctggcgg tcttcgatcg ggcagcgtta
    22561 atgcttatct gcctgttctt tctcatccgt atccgcctgt ttcgcgaact gttgcacaag
    22621 tcggcgcact ccccaaaaga attgctcgcc gttaccgcca ttttctcgct gttcgccctg
    22681 ttcagcacct ggtccggcgt tcccgtagaa ggctcgctgg tgaacgtacg tattatcgcg
    22741 gtgatgtccg gcgggattct gtttggcccg tgggtaggca tcattaccgg cgtgattgcg
    22801 ggtattcacc ggtatttaat tgatatcggc ggcgtgacgg cgatcccctg ctttatcacc
    22861 agcattctgg cgggttgtat atcaggctgg ataaacctga aaatccccaa agcacagcgc
    22921 tggcgctgga tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat
    22981 tacgcaaggt ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta
    23041 ataccgatca tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg
    23101 acttcattag cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca
    23161 atgatgaagc ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga
    23221 aaggggtcgt gaccggaacg ctgaaaattt actactgcca cgcgcatcag atcacctcgt
    23281 cattacagga aatggcggtc ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac
    23341 gcgccgagca gctacgtgaa atggcaaata aggcagagat tcgcgccctg caaagcaaaa
    23401 ttaatcccca ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc
    23461 cggataccgc tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat
    23521 taaaagacga tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg
    23581 ccattgagca ggcccgcttt ggcgacaagc tgacggttat ctatgatatt gatgaagagg
    23641 tgaattgctg cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg
    23701 gtattcagcc ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc
    23761 gggtacgcat tgcggtgcga gataccgggc acggcatcgt tccaaaggtg attgagcggg
    23821 tcgaagcgaa tgaaatgccg ggcaataaaa ttggcctgct gaatgtccat catcgcgtga
    23881 agttattgta tggcgagggg ctgcatatcc gccgcctgga gccggggacg gaaattgcgt
    23941 tttacattcc taaccaacgc accccagtcg cctcacaggc tacgttattg ctttgagccg
    24001 gagtgatatt gtgaaagtca tcattgttga agacgaattc ctggcacaac aggaactgag
    24061 ctggctaatt aaagagcaca gccagatgga gattgtcggc acctttgacg acggtctgga
    24121 cgtgttgaag tttttgcagc ataaccgcgt cgacgccatt tttctggata tcaatattcc
    24181 gtcgctggat ggcgtgttgc tggcgcaaaa catcagccag ttcgcccata aaccgtttat
    24241 tgtgttcatc accgcgtgga aagaacatgc ggtagaagcg tttgaactgg aggcgtttga
    24301 ctacattctc aaaccgtatc aggagtcacg tattaccggg atgctgcaaa aactggaagc
    24361 ggcctggcaa caacagcaga ccagcagtac gcctgccgcg acggtaacgc gtgagaatga
    24421 caccattaat ctggtgaaag atgagcgaat aatcgtcacg ccaattaacg atatctatta
    24481 cgccgaagcg cacgagaaaa tgacctttgt ctatacgcgg cgtgaatcct acgtaatgcc
    24541 gatgaacatt accgaatttt gcagcaaact gccgccgtcg cattttttcc gctgccatcg
    24601 ctcattttgt gtcaatctga acaaaatacg cgaaatcgaa ccgtggttta ataacaccta
    24661 cattctgcga ctgaaagatc tggattttga agtgccggtc agccgcagca aagtgaaaga
    24721 atttcgccag ttaatgcatc tttaatcggg aactttcatg aaagcaccag gcttgcccgc
    24781 cgatcaacaa tttttcgccg atctgttcag cggcctagtg cttaacccgc aactactggg
    24841 gcgcgtctgg tttgccagcc agcctgcctc gttaccggtg ggcagtttat gtattgattt
    24901 tccccgtctg gatatcgtgc tgcgcggcga atacggcaac ctgctggaag caaagcagca
    24961 gcgaatggtg gaaggagaaa tgctgtttat tccggcacgc gcggctaatt taccgatcaa
    25021 caacaaaccg gtgatgctgt taagcctggt gttcgcaccg acctggcttg ggttatcgtt
    25081 ttacgatagc cgcaccacgt cgttgttgca tcctgctcgc cagacccagc ttcccagcct
    25141 gcaacgcggt gaaggtgaag cgatgcttac cgccctcacc catcttagcc gttcgccgct
    25201 ggagcagaat atcattcagc cactggtgtt aagtttgctg catctttgcc gcaacgtggt
    25261 gaatatgccg ccgggtaatt cacaaccgcg cggcgatttt ctctatcaca gcatttgtaa
    25321 ctgggtttag gataattatg cccagccgct cacccgcgag agcgtggcgc agttttttaa
    25381 tatcacgccc aatcatctgt caaaactgtt tgctcagcat ggaacgatgc gttttatcga
    25441 gtatgtgcgt tgggtgcgaa tggcgaaggc gaggatgatt ttgcagaaat atcatctgtc
    25501 aattcatgaa gtggcacagc gttgcggttt tccggatagc gactattttt gtcgcgtttt
    25561 ccggcgtcag tttagtctga cgccgggaga gtacagcgcc cgttttcagg gctaacgtca
    25621 gaaggttaat tctgtttcca gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa
    25681 cgactggcgg aagtttttat tcaccagttt acgcgccagc tgcgagaaga ctttcacatg
    25741 attcatccct tcgttagcac ccagcgtcag catgatcacc agttcgactt cgcccatttc
    25801 tgactgccag tcaaccggtt tcgccagccg ggcaatgctg atactggaat gacggatcca
    25861 ctgagatttg gtgtgcggga tcgctacgcc aaaaccaacg ccggtggtaa caatctcttc
    25921 ccgctgccag acatcttctt ccagctcaaa cggatgttca gtgcgcccgt taacgccgag
    25981 gttgccgcac aggaactgga tcgcctgctc tttattgcta aaatcctgat caacaaagat
    26041 attctccagc gccagcagtg ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc
    26101 ttcaatttcc tgcgcactgc ggcattcaca tgcctgacgc gccagttccc gacacgcctc
    26161 gctatccagt tgacgaagct ggcttttcac cgccggaata cgcgggctac tcatactcag
    26221 ctcatccagg cccagcccaa gcagtagcgg cagataacgg ctttcaccgc ccagttcacc
    26281 gcaaatgcct acccatttgc cccgctgatg cgcagtggta actatttgct gcaacatgcg
    26341 caggaacgat ggcgtaatcg ggttatatag cggcgatacg cgcgggttat tacgatcgac
    26401 cgcatacaga tactgggtca tatcgttgga gccgatactg aagaaatcga cctcatcgca
    26461 gaagtggtcg atgatgtagc acaccgacgg cacttccacc atgatcccaa gcgtaatcgt
    26521 ctctgcatga cgcaggccat cgcgcttaag ctcaacgatc gctttttgga tctcgccttt
    26581 cacccataag atctgatcga ggctgtgaac catcgggatc atcaactggg cgttgccgaa
    26641 actggcggcg cgcaaaatgg cccgcagttg agtgcggaac aggccagcaa attccgggta
    26701 aatacgtacc gcgcgatagc cgaggaacgg gttctcttcc tggggaatat tcagataagg
    26761 aatgctttta tcgccgccga tgtccatcgt gcggaagata atcggcttgt cgcccgccgc
    26821 cagtagcacc tgctggtagg cttcaaattg ctcctgctcg tccggcgcgc tgtcgcgatc
    26881 catatagagc atttcggtac ggaacagccc gacaccttcc gcgccgttgg caaacgcgcc
    26941 tggcgcttcc agagcggtgc cgatattcgc cgcaatatca atacgcttgt tatcacggga
    27001 ataggccagc tgcgcggcag cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg
    27061 cgcgacctga taataaccgc tcaccgcgtc attcgggtta atcgccagca cgccgcactg
    27121 ggcgtcaagc actgcaggtt gcccggcata acgggcaatc gcatccagcg gcaagccact
    27181 cagtaccggg atcgccgagg cacgggccag aatcagtgta tgcgaggtgc gcccggtttt
    27241 ctccagaatc atacccgcaa gatttttcaa atcaaggctc aaaaactggc ttggggttaa
    27301 atcttcagcc accagaatgg tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc
    27361 cggccaggtg atatgcagca actgttcgct gatatcgcga atgtcgctga cccgctcgcg
    27421 cagataatcg ctggcagaag cagatagttt ggcgcaaacc tgctccatat tgctgatgat
    27481 cgccgccccc agtccctgat gctgttctgt catcaggcga cggatattgc ctgcaaattc
    27541 atcatcctga atcagcgaca aatgagcgct gaggatagtt ttgctttcgc cgtcacgctc
    27601 acgcaattgc tgattcagtt gctcggcaag cgttgccagg ctgtgctcca gccgggtgga
    27661 atcttgcgca ctggcgggga ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt
    27721 cagcgtaccc acgccgacgc cgcttgccag cacattgccg tacagtaaat ccgggttaag
    27781 gcggcttaat gaacgcggca gcggatgcgc cgtcagttct gcctgcgtcg gctgaacgct
    27841 gtcgctgtcg ataaagcgca ccaggatgta ctcttccagc acgcgccgcg cctgctcttc
    27901 atcgctgccg ctaatgttca ggctgcaact gtcattaaat aaggtgccag ttccaatcaa
    27961 cgccagcgag cttttagcat cagcctttgc gttctggcga tggttgataa aggtgatttc
    28021 gctttgccac tggctgcact gttctttaag ttcccacgcc ggacgagcat gtagaccgtt
    28081 aggcaaagga cagagaaatt gaatcgttaa cataaccgct ccgggttaat caggagatca
    28141 tctgaaatcc gtcagttgaa caaccgtctc acgcgtaaga cgttgaataa gggcagataa
    28201 aagttgctcc atctgcaaaa tgtcgcggca atcggcaatc gatgccgcgc aatgtccgtg
    28261 gcgggtggca ggccccatta ccaatgtggg tacgccagta ccggttaagt gcaccgctcc
    28321 accgtccgtg ccgccgttac tgaacatatc cgcctgcaac ggaacgccaa tttctgccgc
    28381 cacggtttca atccacgcgg taagttttgg cggcgcaatc agcgatttat cgcttaacac
    28441 cagcatcggc ccgttaccaa tctgacgatg gttagccgcg ccataatcaa agtttttcgc
    28501 ccagcaggcg gtatcaagca caatggcgac gtccggcgac accgcgcggg tagcagtttg
    28561 cccgccgcgt aatcccacct cttcgctgga acttgccacc agccacactt ccgcaggcag
    28621 ttcggcgtcg tgcagctcac gtagtaacgt caccagcaga tagcaactga ggcgatcatc
    28681 aaaggctttg cccatcaccc gctggtgagg gaggacctga aaagtggtat cgaaggtgac
    28741 gcgatcgcct ggacgaattc ccgcctgcat cacttcgtca taggtgcgcg cgccaatgtc
    28801 cacgcgcatg gcgctaacgt cattcccctg ccggtcgccg tcaagcaggc ctggaatttt
    28861 gcactcttca cgggtggtga tgcgcaccgg ctgcagctgg cgggcagcca tgcgcacgtt
    28921 gccaaccggc agcacgtcaa tagcgccttc gcgggagatg ctgcgcacca taaatcccac
    28981 ttcgtccata tgggcgcaga tcatcacctt cggacctgtc gattcattga ggcggatcag
    29041 caccgatccc agaccatcaa atcgcacttc tttttgcagg cgagccgctt cttccagcag
    29101 gatctgccgc acttcctgtt ccgaggaggc gatcgcatct gcctcgctca acgcttttaa
    29161 tagcgataaa tccattatgc ctctcccgtg agcaacactg ttttcggcat ggcgtagagc
    29221 acttctgcgc cttgcggggt aaccagcaca acatcttcga tgcgcacgcc cccttgccct
    29281 agcaaataaa tccccggctc cacggtcagc aacatgcctg gctgtagcgt cgtggtgtcc
    29341 cgcggtgaaa aacgcggatc ttcatggact tcaatgccga tagcgtgacc agtgttatga
    29401 ccgaaatagt cgccaaatcc agcctcggta atgacccggc gcgcggcgtc gtcaatctgc
    29461 tggcagcgca cgccggggcg aattgcagag attgccgcga gctgtgcctg caggacaatc
    29521 tgatagacgt caaacagcgg gtgagattcg gcgctcaccc cttcgccatt caccagcaag
    29581 gtgcgcgtca tatcagcagt agccctgata cagcgcacca aaatcgagag tgacaaactc
    29641 gcccgctgca acaatcttgt cgctggcttt gccgtgcggc agcgccccgc gccagccgct
    29701 ggcgacaatg gtgtcaaaag aggctttttc tgcgccctgc tggcgcataa accactccag
    29761 ttcagcggct atctcgcgct cgctcatccc cgcctgaata aagcggcgaa tatgctccgc
    29821 tccgcgatcg gcaatcccac aggcgaggcg gattatctcc acctcctctg gcgttttgat
    29881 ttgccgcagc acatccggcg tggcgctaac cagtttcgca ttgagttcag acttccagcg
    29941 gtgcgcggtt tcccaactca cctgctggcc ttcaaaaccg agcgtttgca actgctcatc
    30001 ggcaatgatt tgattgacga tagtggtaag cgtgttcgtt gcgtcaagca aatgcagctg
    30061 gtagccttgc gcacgggctt ccacctcaac gaaatagcgc gaatctacca gaatgtgcgc
    30121 actttcacgg ctaataacca catagcctga tccggtggag atccccagat gcggctgttt
    30181 gttctgccgt gaggagagaa gcaccgcatc cagttgttgc accttaagcc agttgcgcag
    30241 cgaagcgagt aatgtcatct ggattttcct tcttacaggc tatcgattaa caatttgcct
    30301 ttgcggaaca tcatcagacg caggaacacc accatcagcg cagtaatgat tgcccccagc
    30361 gcgatccccg ccatataaac gcccaagtta gtgaccagcg gccatgccca gatagcggat
    30421 tccgggaacc attgcactgc gcccagccag acagctgcgg ttgagccgac aatcgcgccg
    30481 accatatatg acggaatagc ggtgatgggg ctttccagcg caaacggaat cgcaccctca
    30541 ctaatcccca taaaggccag gaacatcgcg gtcttaccct gcggataaag ttgagcgttg
    30601 aacaggcgtt tgccggttaa acgacggtca ataatggtcg ccagacccag accaatcggc
    30661 ggaataacga tagcgataga acgagcggtg accggtaata cgtggtcagt ggtaaagctg
    30721 aaggcaacga aacctgcggc tttgttgatc gggccaccaa ggtcgattgc tgtcgccgcg
    30781 gcgatcccca ttgcgtacat cagcgcgccc ttctcacctg cggcagtcag cacagtacgg
    30841 ataccgccgt tgatccagcc accaaaggga gtgatgacgt agtacatcgc cagcatgaca
    30901 aaaatggcgg aaagaatcgg tagcaaaaat gtggttttga acgctaacag gaaatccggt
    30961 aactggattt tctggttcat ccatttcaca aggtaaccgg caacgataga aataatcagc
    31021 gcaccaatga aggtggacgg caccggcgaa gaggtcgccc attgcatagt gctgggatcg
    31081 aagttcagca gttgcgtcgg ctgggtagac atcaacccgc cgataaaacc agccgggaac
    31141 gccagtttgc caccgataga gttcgccaca aatgcagcaa acatcggaat ggcaaaacca
    31201 aacaacacgc cgccgaagga ctgcgacagc caggcaaatt taagtaagga gaggtcgaaa
    31261 ccggagaatt tcccgctatt aagagcatcc atgatgccga tctcagcagg aattttaagc
    31321 cagctataag caatcagctg gctgaaagcg aggatcacac cgcccataat taaggtcggc
    31381 accatgcgtg aaatccctga catcacatgc tgcgggagtt caccccagaa gctgctttta
    31441 gaggcctgcg gattcttaac tgccgctgcc gcaccggatg cgccaggcac aacggttgca
    31501 ctgcgttttt taatggccat aacaattatc cctgtcgaat attactgttg ttcagaggca
    31561 atcatctctt cgatttcttt gatgatgccc gcagcgtttt taattgcgtc ctgcaaagtg
    31621 atttcataaa cgtcgcgtga ttcgaaacgt tcgttatctt ccggggtaac tgccacggaa
    31681 tggatgatga tggtcgcttc ggcgatatcc tgcgccgtca ggcgattctg gatgccgtcc
    31741 gcgccctggg tttcaatttt cacttcataa ccggcttcta ccgccgcttc ttccagcacc
    31801 tgagcggcca taaaagtgtg agccaggccc attgggcagg cacataaggc aatcagtttc
    31861 ttactcatcg ttaattcctc gttcagtgat agttgactag tgaaattatg aacgtgcaaa
    31921 acaaatcgcc gttaccagac aaataatgca tttactggag aattaatccc gctgtacgga
    31981 agtgtgacgc aggtcggcct tgtgagggga cacaaagaaa aattgaatta actgtatgat
    32041 ttaaaagatt atcgggagag ttacctcccg atataacagg aaggatttac agaatgtgac
    32101 ctaaggtctg gcgtaaatgt gcaccggaac cgagaagacc cggatagtca tggacgatga
    32161 gatacaccgg aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac
    32221 ggaaaccgga ggctttgaag aactcaagga agcgcggcac gataccgccc gcaataaaca
    32281 cgccgccaaa tgtcccgaga ttgagcgcca gattgccgcc aaaacggccc ataatgacgc
    32341 aaaacagcga caatgcgcgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa
    32401 tatcttttgg cttgagattt tctggcaggc ggttgtcagc tttcacaatt gcgcgataca
    32461 aattcaccag cccagggcca gaaagcacgc gctccgccga aacatgacca atttccgcac
    32521 gcaatatttc gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc
    32581 cttcgcccgg caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc
    32641 ccgttccggc accgtaaacc gcaataggct taccttcgac cggttctgcg ccaccaaact
    32701 gaatcagatg ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa
    32761 taatttccag atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg
    32821 tatggttggt cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt
    32881 ctttcacctc gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt
    32941 aatcaagccc tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag
    33001 caagacgtgc gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac
    33061 tgctccgcta aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac
    33121 aacgccggaa aggcaggctc cctgtaaata tcgatctggg tcacacaatt actttatcgt
    33181 ttcagcacca attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg
    33241 cataaggaat agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc
    33301 gccgtggcaa ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg
    33361 gtattacaga atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca
    33421 cccctctgga tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt
    33481 ttcagccagg gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccggtt
    33541 ccgccctctg cgctacctag acttatcgta gcattaattc tcggtcttgc tggcggcgtc
    33601 agcctggggc cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc
    33661 gctcgtctgt taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc
    33721 atcggcgcgc tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat
    33781 ggcagtagtg aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct
    33841 ggtgcactta ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac
    33901 ggacagatgg aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca
    33961 gcagggatgg tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa
    34021 aatccggtgc tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt
    34081 ggaccagttt cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct
    34141 ttcagcacca gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct
    34201 gccgccagtg gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta
    34261 gggttgatgc tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct
    34321 attctcggca tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca
    34381 gtcgttgtac ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg
    34441 ctgttattag caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac
    34501 gataatgttt agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg
    34561 gctttacctc cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga
    34621 ataacaatgt tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca
    34681 gcaaatgcag gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat
    34741 cattacggta attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat
    34801 tatgaatggc gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat
    34861 aagcgtaaag catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc
    34921 ggaaaaggcc gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc
    34981 tgatgcgacg ctagcgcgtc ttatcaggcc tacaaaccgg ctctttaacg cgtagggcgg
    35041 ataaggcgtt tacgccgcat ccgccaatag tgccagatgc gacgctcatt caactacaat
    35101 cccagcgccg tccccaccaa caaccagata ttcagcgcca cgaccagcac tacaatcacc
    35161 cagcctgtct gttttacgcg tttgctgttc accagatcgc ccatcaactt gctgtcactg
    35221 gtgaaaatca gcagtggaac cagcgccagg gcgataccaa aacttaacag cacctgactc
    35281 ataaccagaa tccgtgtcgg atctaatccc atcagaatga caataaatga cggcaacatg
    35341 gtgactgtac gacgcaccca cagcgggata tggaagcgaa tgaatccctg catcaccacc
    35401 tgccccgcca gtgtccccac caccgttgag gacagtccgg cagcaaccag acttaaccca
    35461 aagaccgttg ccgcagcatg gcttaacagc ggttgcagcg tcagataagc ctcatcaaga
    35521 tcggcaacac cagtatgacc agaaaagtgg aacgccgccg cagctgtagc catcatcacc
    35581 agattgacaa aaccggcaat cgtcatggcg atagccacat cccatttggt ggcggaataa
    35641 cgttgttgac gcgaaccgcc atgtaaatgc tgagtgagcg aagagtgcaa ataaatcaca
    35701 tgcggcataa tcgtcgcccc taacacgcct gctgccagga agaccgcctc cgaagtaggt
    35761 aaactcggga tcaccattcc tttacccagc tgcgccaggt taggctggga gaaaatcaac
    35821 tcgacaatgt aagccgcggc aacaaacaac agtaacccgc caatcacttt ctccagcggt
    35881 ttttgcccgc gacgttgcag cattaaaatc aggaaagtcg cgatccccgt cagcaccgcg
    35941 ccctgcaaca acgaaacacc aagaatgagt ttaaaaccga tcgccgcacc aataaattcc
    36001 gccaggtcgg ttgccatcgc aataatttct gcctgaaccc aatagaacca cactacggga
    36061 cgcggatagt gatcacgaat ctgctccgcc agatttttac cggtggcaat ccctagtttg
    36121 gcagagagga tctgaatcag catcgccatc aggttggccc aaacgacaac ccacagtagc
    36181 tgatagccga agctagcacc cgcctgaata ttggtcgcaa agttaccggg atcgatataa
    36241 ccaatcgccg caatgaacgc aggtcccatt aatgcgagcc tcatcttacg cgccgcccgt
    36301 ccgctgctac tctcaacgcg atagttcgtc atcttgtgcc tctaaaacat agcctttgct
    36361 atgtttcatg ctatgctaaa cgagaatgat tatcaaattc atttaaatgg attgtggtga
    36421 tttctctgat agaccaagat tatgattacg aaaagattga tgacttgaat gttatggtta
    36481 tgtttaatgt tagcacattt acataacttt cagcttccat acacaacata gcagaaatgt
    36541 atgacagatc actatttttg aagcctgtca caggacgtca ttatagtgtg tgtcagatct
    36601 cgttttcctt aaccatgtta catagaatgt gcacggaaat ttaacctgcc tcatatttgg
    36661 agcaaatatg gaccgcgtcc ttcattttgt actggcactt gccgttgttg tgattctcgc
    36721 actgctggta agcagcgacc gcaaaaaaat tcgtatccgt tatgttattc aactgcttgt
    36781 tatcgaagtg ttactggcgt ggttcttcct gaactccgac gttggtttag gcttcgtgaa
    36841 aggcttctcc gaaatgttcg aaaaactgct cggatttgcc aacgaaggga ctaacttcgt
    36901 ctttggtagc atgaatgatc aaggcctggc attcttcttc ctgaaagtgc tgtgcccaat
    36961 cgtctttatc tctgcactga tcggtattct ccagcacatt cgcgtgttgc cggtgattat
    37021 ccgcgcaatt ggtttcctgc tctccaaagt caacggtatg ggcaaactgg aatcctttaa
    37081 cgccgtcagc tccctgattc tgggtcagtc agaaaacttt attgcctata aagatatcct
    37141 cggcaaaatc tcccgtaatc gtatgtacac catggctgcc acggcaatgt ccaccgtgtc
    37201 gatgtccatc gttggtgcat acatgaccat gctggaaccg aaatacgtcg ttgctgcgct
    37261 ggtactgaac atgttcagca cctttatcgt gctgtcgctg atcaatcctt accgtgttga
    37321 tgccagtgaa gaaaacatcc agatgtccaa cctgcacgaa ggtcagagct tcttcgaaat
    37381 gctgggtgaa tacattctgg caggtttcaa agttgccatt atcgttgccg cgatgctgat
    37441 cggctttatc gccctgatcg ccgcgctgaa cgcactgttt gccaccgtta ctggctggtt
    37501 tggctacagc atctccttcc agggcatcct gggctacatc ttctatccga ttgcatgggt
    37561 gatgggtgtt ccttccagtg aagcactgca agtgggcagt atcatggcga ccaaactggt
    37621 ttccaacgag ttcgttgcga tgatggatct gcagaaaatt gcttccacgc tctctccgcg
    37681 tgctgaaggc atcatctctg tgttcctagt ttccttcgct aacttctctt caatcgggat
    37741 tatcgcaggt gcagttaaag gcctgaatga agagcaaggt aacgtggttt ctcgcttcgg
    37801 tctgaaactg gtttacggct ctaccctggt gagtgtgctg tctgcgtcaa tcgcagcact
    37861 ggtgctgtaa aaccatacat aaaaaagccg gggatcattc cccggctttt ttacgcctgt
    37921 taatcagcta atggctgcgg gcgaccaatc aaataccctt gcagatattg caccccgagc
    37981 ttatgcaata gcgcctgctg ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac
    38041 ttcgctttcg ccagatcggt aattgatctc acaatcatcg catccagcgt attcgtgaca
    38101 atatctttca caaagacgcc atcaattttg atgatatcag cctgcaaacg ctttaaccgt
    38161 tcgtagttgg catatccggt gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc
    38221 agctgctcga tgttgtacat gctgctttct gcgttagaaa acgcctgctc ctcggtgatc
    38281 tcaagaatga ccgcctgcgg ggagatgtga taacgcttaa acagacgaat aatccgcccg
    38341 gcaatattct tttgcagcag cgtgagcggc attaaattga ctgaaaagcg cggtcctttt
    38401 ttgtcgcaag ggtgtgttgc cagccacttc aacaaggatt ccagcacttg caaatcaaaa
    38461 cgcgcgctaa ggttaaactg agcaataagg ggcagaaact tatccggggt cataatgccg
    38521 ccgtcatatt tcagtcgcgc gaggatctca tcataacctt caccctcttt gttgcgaatt
    38581 ggctgggcgt agagcagcaa atcaccctga tctaacgccg tgcgaatggt attcagcaat
    38641 agcacctgtt tagtggtctg cccggaaacc atctcctctc tgctatccag cgccagcaca
    38701 tgatgatgtg cgcaggattg ctccgccagc cagcttaact gccccaacaa gggttgcagg
    38761 gtttcctgat ttccatcaaa acgcccccag gcagcaccat agcccatatc cagcccggta
    38821 ttgttccagt gaatttgccg actattcagg atgttaacca tatgctggag tcgcccttcc
    38881 gtttccggcc cgctcagcac taacagcagt tcactacccg gcaattgata caacttttcg
    38941 ttttcctgca tcaacggcag cagcgtacgg caaattgagc ggatacagtg aacgcgcatc
    39001 attaagccgt aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaaactc
    39061 ttgcccgcct cttgctccgg cgcttgttcc aacgcacgaa agttgggtag gagcgttaac
    39121 ggatctgtta gcgcctgcaa atgccagcgg cgattaagcc attcactgcg atgataaatg
    39181 cgcaccatat agagcaggca aacgctaaag gaaatcagca ccgcgagaat aaatgccagc
    39241 gaatattcgg tttccacccc ttgcaaaaag ttctgattgt aattcagaag gcaaagcgtt
    39301 gaaacagccc aggtgagatt taaaaacgga tagcgaagat taccgacccc gagggtaaag
    39361 atgatgaaga aaacgggtac caggtaaccg gcaataaagt cgttttcata aggtgtgcac
    39421 aacagaagta gcagcacgct taacgctgcc agccagctta aggtaaatgc gagtgagata
    39481 gtagaaaagc atgttgtaaa tcagcactgc ggtgaaaagg cttagtaaat caacgaccgt
    39541 gaaaatggca tccgcatcgc cgaaaaaggt ggatatcttt agcgggaaat caaagaagct
    39601 ccccacaaga tacatgctgc atttgatgcc gatcggcgtc accaaaccaa gccagaacag
    39661 acgttgccag acatatcggc tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg
    39721 taatattgcg caggcacaca acacgacaaa cgtctggctg ccgagcatga ccgcagtctg
    39781 taaaaaagag agatgaaagt tccacagatt ggtgcaaaac atcccgcata aaacgggaac
    39841 gacaccgcgc cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc
    39901 cagaaagata taactggaat ggataatggc aagtggcgaa ataaaacggg atagctgaat
    39961 aagtaccacg gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg
    40021 ctccacaaac atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa
    40081 gtttattcgc atcgcgaagc aaacaggggc cagcctgttg ccgatttttc atccaatgat
    40141 gctttcacca ggcggcgcta acccggacgt agactgtatt acaaaagcgg caaaaagcag
    40201 agacaaaaaa cccccgcttt gcagcgaggg ttggaaattt ggtgg
//
LOCUS       DAJTYH010000026        38767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTYH010000026 DAJTYH010000000
VERSION     DAJTYH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            183..1886
                     /gene="uidA"
                     /locus_tag="OO609_001346"
     CDS             183..1886
                     /gene="uidA"
                     /locus_tag="OO609_001346"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6632826.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            1892..2917
                     /gene="uidB"
                     /locus_tag="OO609_001347"
                     /pseudo
     CDS             1892..2917
                     /gene="uidB"
                     /locus_tag="OO609_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            2956..4221
                     /gene="uidC"
                     /locus_tag="OO609_001348"
     CDS             2956..4221
                     /gene="uidC"
                     /locus_tag="OO609_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6632827.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(4447..5955)
                     /locus_tag="OO609_001349"
     CDS             complement(4447..5955)
                     /locus_tag="OO609_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6632828.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(6056..7231)
                     /gene="manA"
                     /locus_tag="OO609_001350"
     CDS             complement(6056..7231)
                     /gene="manA"
                     /locus_tag="OO609_001350"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6632829.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7430..9076
                     /gene="fumB"
                     /locus_tag="OO609_001351"
     CDS             7430..9076
                     /gene="fumB"
                     /locus_tag="OO609_001351"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6632830.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            9219..10622
                     /gene="fumC"
                     /locus_tag="OO609_001352"
     CDS             9219..10622
                     /gene="fumC"
                     /locus_tag="OO609_001352"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6632831.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(10619..11548)
                     /gene="tus"
                     /locus_tag="OO609_001353"
     CDS             complement(10619..11548)
                     /gene="tus"
                     /locus_tag="OO609_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6632832.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(11624..12925)
                     /gene="rstB"
                     /locus_tag="OO609_001354"
     CDS             complement(11624..12925)
                     /gene="rstB"
                     /locus_tag="OO609_001354"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6632833.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(13104..14423)
                     /locus_tag="OO609_001355"
     CDS             complement(13104..14423)
                     /locus_tag="OO609_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6632834.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(14648..15391)
                     /gene="rstA"
                     /locus_tag="OO609_001356"
     CDS             complement(14648..15391)
                     /gene="rstA"
                     /locus_tag="OO609_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6632835.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            15520..15855
                     /locus_tag="OO609_001357"
     CDS             15520..15855
                     /locus_tag="OO609_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6632836.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(15852..16574)
                     /gene="folM"
                     /locus_tag="OO609_001358"
     CDS             complement(15852..16574)
                     /gene="folM"
                     /locus_tag="OO609_001358"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6632837.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(16611..17993)
                     /locus_tag="OO609_001359"
     CDS             complement(16611..17993)
                     /locus_tag="OO609_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6632838.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(18179..19123)
                     /locus_tag="OO609_001360"
     CDS             complement(18179..19123)
                     /locus_tag="OO609_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6632839.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            19647..21179
                     /gene="pntA"
                     /locus_tag="OO609_001361"
     CDS             19647..21179
                     /gene="pntA"
                     /locus_tag="OO609_001361"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6632840.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            21190..22578
                     /gene="pntB"
                     /locus_tag="OO609_001362"
     CDS             21190..22578
                     /gene="pntB"
                     /locus_tag="OO609_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6632841.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(22603..23637)
                     /gene="tqsA"
                     /locus_tag="OO609_001363"
     CDS             complement(22603..23637)
                     /gene="tqsA"
                     /locus_tag="OO609_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6632842.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            24048..24413
                     /gene="mdtJ"
                     /locus_tag="OO609_001364"
     CDS             24048..24413
                     /gene="mdtJ"
                     /locus_tag="OO609_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6632843.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            24400..24729
                     /gene="mdtI"
                     /locus_tag="OO609_001365"
     CDS             24400..24729
                     /gene="mdtI"
                     /locus_tag="OO609_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6632844.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(24768..25589)
                     /locus_tag="OO609_001366"
     CDS             complement(24768..25589)
                     /locus_tag="OO609_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6632845.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(25689..25772)
                     /locus_tag="OO609_001367"
     CDS             complement(25689..25772)
                     /locus_tag="OO609_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632846.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(25865..26200)
                     /locus_tag="OO609_001368"
     CDS             complement(25865..26200)
                     /locus_tag="OO609_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6632847.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(26597..27850)
                     /locus_tag="OO609_001369"
                     /pseudo
     CDS             complement(26597..27850)
                     /locus_tag="OO609_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            27957..28868
                     /locus_tag="OO609_001370"
     CDS             27957..28868
                     /locus_tag="OO609_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6632848.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            29003..30223
                     /gene="mlc"
                     /locus_tag="OO609_001371"
                     /gene_synonym="dgsA"
     CDS             29003..30223
                     /gene="mlc"
                     /locus_tag="OO609_001371"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6632849.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            30348..31043
                     /gene="bioD"
                     /locus_tag="OO609_001372"
     CDS             30348..31043
                     /gene="bioD"
                     /locus_tag="OO609_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6632850.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(30996..32288)
                     /gene="clcB"
                     /locus_tag="OO609_001373"
     CDS             complement(30996..32288)
                     /gene="clcB"
                     /locus_tag="OO609_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6632851.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(32447..33061)
                     /gene="dmsD"
                     /locus_tag="OO609_001374"
     CDS             complement(32447..33061)
                     /gene="dmsD"
                     /locus_tag="OO609_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6632852.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(33104..33958)
                     /locus_tag="OO609_001375"
     CDS             complement(33104..33958)
                     /locus_tag="OO609_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6632853.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(33960..34577)
                     /gene="dmsB"
                     /locus_tag="OO609_001376"
     CDS             complement(33960..34577)
                     /gene="dmsB"
                     /locus_tag="OO609_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6632854.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(34588..36626)
                     /locus_tag="OO609_001377"
                     /pseudo
     CDS             complement(34588..36626)
                     /locus_tag="OO609_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(36687..37796)
                     /locus_tag="OO609_001378"
     CDS             complement(36687..37796)
                     /locus_tag="OO609_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6632855.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            37779..>38767
                     /locus_tag="OO609_001379"
     CDS             37779..>38767
                     /locus_tag="OO609_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6632856.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRA"
BASE COUNT         9848 a         9403 c         9944 g         9572 t
ORIGIN      
        1 ttaatgcgat ccatatcacg ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc
       61 agttcttttt atttccattt ctcttccatg ggtttctcac agataactgt gtgcaacaca
      121 gaattggtta actaatcaga ttaaaggttg accagtattt ttatcttaat gaggagtccc
      181 ttatgttacg tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg
      241 cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg ttggtgcgaa agcgcgttac
      301 aagaaagccg ggcaattgca gtgccaggca gttttaacga tcagttcgcc gatgcagata
      361 ttcgtaatta tgtgggcaac gtctggtatc agcgcgaagt ctttataccg aaaggttggg
      421 caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca
      481 ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc gatgtcacgc
      541 cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaac gaactgaact
      601 ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt
      661 acttccatga tttctttaac tatgccggga tccatcgcag cgtaatgctc tacaccacgc
      721 cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg
      781 cgtctgttga ctggcaggtg gtggccaatg gtgatatcag cgttgaactg cgtgatgcgg
      841 atcaacaggt ggttgcaact ggacaaggca ccagcgggac tttgcaagtg gtgaatccgc
      901 acctctggca accgggtgaa ggttatctct atgaactgtg cgtcacagcc aaaagccaga
      961 cagagtgtga tatctacccg ctgcgcgtcg gcatccggtc agtggcagtg aagggcgaac
     1021 agttcctgat taaccacaaa ccgttctact ttactggctt tggtcgtcat gaagatgcgg
     1081 atttgcgagg caaaggattc gataacgtgc tgatggtgca cgatcacgca ttaatggact
     1141 ggattggggc caactcctac cgtacctcgc attaccctta cgctgaagag atgctcgact
     1201 gggcagatga acatggcatc gtggtgattg atgaaactgc agctgtcggc tttaacctct
     1261 ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga actgtacagc gaagaggctg
     1321 tcaacgggga aactcagcag gcgcacttac aggcgattaa agagctgata gcgcgtgaca
     1381 aaaaccaccc aagcgtggtg atgtggagta ttgccaacga accggatacc cgtccgcaag
     1441 gtgcacggga atatttcgcg ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc
     1501 cgatcacctg cgtcaatgta atgttctgcg acgctcacac cgataccatc agcgatctct
     1561 ttgatgtgct gtgcctgaac cgttattacg gttggtatgt ccaaagcggc gatttggaaa
     1621 cggcagagaa ggtactggaa aaagaactgc tggcctggca ggagaaactg catcagccga
     1681 ttatcatcac cgaatacggc gtggatacgt tagccggcct gcactcaatg tacaccgaca
     1741 tgtggagtga agagtatcag tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg
     1801 tcagcgccgt cgtcggtgaa caggtatgga atttcgccga ttttgcgacc tcgcaaggca
     1861 tattgcgcgt tggcggtaac aattgaccta catgggcctc gggctttgct acagcctggt
     1921 gaatattcct tatggttcac ttgctaccgc gatgacccaa caaccacaat ctcgcgcccg
     1981 tctgggcgcg gctcgtggga ttgccgcttc attgaccttt gtatgcctgg catttctgat
     2041 aggaccgagc attaagaact ccagcccgga agagatggtg tcggtatacc atttctggac
     2101 gattgtgctg gtgattgccg gaatggtgct ttacttcatc tgcttcaaat cgacgcgtga
     2161 aaatgtggta cgtatcgtgg cgcagccgtc attgaagatc agtttgcaaa ccctgaaacg
     2221 gaatcgcccg ctgtttatgt tgtgcatcgg tgcgctgtgt gtgctgattt cgaccttcgc
     2281 ggtcagcgcc tcgtcgttgt tctacgtgcg ctatgtgtta aatgataccg ggctgttcac
     2341 tgtgctggta ctggtgcaaa acctggttgg tactgtggca tcggcaccgc ttgtgccggg
     2401 gatggtcgcg aggatcggta aaaagaatac cttcctgatt ggcgctttgc tgggaacctg
     2461 cggttatctg ctattcttct gggtttccgt ctggtcactg ccggtggcgt tggttgcgtt
     2521 ggccatcgct tcaattggtc agggcgttac tatgactgtg atgtgggcac tggaagctga
     2581 taccgtagaa tacggtgaat acctgaccgg cgtgcgaatt gaagggctca cctattcact
     2641 attctcattt acccgtaaat gcggtcaggc aatcggaggt tcaattcctg cctttatttt
     2701 gggattaagc ggatatatcg ccaatcaggt gtaaacgccg gaagttatta tgggcatccg
     2761 cacatcaatt gccttagtac cttgcggatt tatgctactg gcattcgtta ttatctggtt
     2821 ttatccgctc acggataaaa aattcaaaga aatcgtggtt gaaattgata atcgtaaaaa
     2881 agtgcagcag caattaatca gcgatatcac taattaatat tcaataaaaa taatcagaac
     2941 atcaaaggtg caactatgag aaaaatagtg gccatggccg ttatttgcct gacggctgcc
     3001 tctggcctta cctctgctta cgcggcgcaa ctggctgacg atgaagcggg actacgcatc
     3061 agactgaaaa acgaattgcg cagggctgat aagcccagtg ctggcgcggg aagagatatt
     3121 tacgcatggg tacagggagg attgctcgat ttcaatagtg gttattattc caatattgtt
     3181 ggcgttgaag gcggagcgta ttatgtttat aaattaggtg ctcgtgctga tatgagtacc
     3241 cggtggtatc ttgatggtga taaaagtttt ggctttgccc tgggggcagt aaaaataaaa
     3301 cccagtgaaa atagcctgct taaattaggt cgcttcggga cggattatag ttatggtagc
     3361 ttaccttatc gtattccgtt aatggttggc agttcgcaac gtacattacc gacagtttct
     3421 gaaggagcat taggttattg ggctttaacg ccaaatattg atctgtgggg aatgtggcgt
     3481 tcacgagtat ttttatggac tgattcaaca accggtattc gtgatgaagg ggtgtataac
     3541 agccagacgg gaaaatacga taaacatcgc gcacgttctt ttttagccgc cagttggtat
     3601 gatgatacca gtcgctattc tctgggggca tcggtacaga aagatgtttc caatcagata
     3661 caaagtattc tcgagaaaag cataccgctc gacccgaatt atacgttgaa aggggagttg
     3721 ctcggctttt acgcgcagct cgaaggttta agtcgtaata ccagccagcc caatgaaacg
     3781 gcgttggtta gtggacaatt gacctggaat gcgccgtggg gaagcgtatt tggcagtggt
     3841 ggttatttgc gccatgcaat gaatggtgcc gtggtggata ccgacattgg ctatcccttt
     3901 tcattaagtc ttgatcgtaa ccgtgaagga atgcagtcct ggcaattagg tgccaactac
     3961 cgtgtaactc cgcaatttac gctgacattt gcaccgattg tgactcgcgg ctatgaatcc
     4021 agtaaacgag atgtgcggat tgaaggcgcg ggtatcttag gtggtatgaa ctatcgggtc
     4081 agcgaagggc cgttacaagg gatgaatttc tttcttgctg ccgataaagg gcgggaaaag
     4141 cgcgatggca gtacgctggg cgatcgcctg aattactggg atgtgaaaat gagtattcag
     4201 tatgacttta tgctgaagta agaaataacg ccggagagaa aaacctccgg cgtttcagat
     4261 tgttgacaaa gtgcgcgttt tttatgccgg atgcggcgta aacgccttat ccagcctaca
     4321 aaaactcata aattcaaagt gttgcaggaa aaggtaggcc tgataagcgt agcgcatcag
     4381 gcaatctctg gtttgttttc agatgaaaac gccggagtga aaattctccg gcgttttggc
     4441 cgtgaattac tgctgcggaa ttgccggtac agccggaacg ttaagagctg gcatcgcaaa
     4501 catgccaaca aaatcttcta acgacatttt ctgcccattt aacgttatct gaccgttagc
     4561 atattgcagg ctggtggtga tggtattgtc ctgcaaggtg gtcagacgga acatctgccc
     4621 cattgctgat gcaccttcaa cttgctgttt cgccagtttt ttcgcttgat cttcctgata
     4681 accttccagc ttcgctacct gagtcatcaa ctcagttgcc atatccaccg gaatggtcag
     4741 tttcgcatcc agagatttaa ccgaacgatc tacttcctgc gccagcgttt gcggcgcttc
     4801 tttagtcgtt gccggatctt tcaggaacag cgacagattc agcgcacttt caccctgact
     4861 gtttttccag cttagcggcg cgatagtaat caccggatcg cctttcaaca tcagcggcag
     4921 ggcgctaaag aaggcttccg tcactttctc ctgataaagt tcgggattgt tggcaatttc
     4981 tggctgtgcc agcagcgcct gagtttgcgc gttatattgc tggctaaact gatgccaggc
     5041 ttcaccatca atctggccga cttttaaagt cagcttgccg ctgcccagat cctgattctg
     5101 taccttcagg ctgtttagcg agtaatccag ttggctattg atcgttttac cgtcattgac
     5161 cagatccgat ttaccgctga tctccatgcc ttccagcagt gccagttctt tgccttccac
     5221 tgaaatggtc attttttcca gtgacagttt ttggtttcct acgcgctcac caaaacttcc
     5281 cagcgtgctg gaaccgtcgg ttttcagatt attaaaggtc aactgcactt tctggttgta
     5341 ttcgttaact gcgtctatcc gaccactttg cgcctctccg gaaagggaga tggctttgcc
     5401 gtctctgtca gcatttaact ggaactcgcc gccgctaaag gcgacttttt catccttttg
     5461 ctcgtaattc agtggattga gcgaaatatc ggaactggaa tcaccgctgt aaccaatgcg
     5521 cgagttaatc tcaaaaggcg tttcaccttt tgccatatca aacagtggtt tgcttacttc
     5581 gttattaacc agcgtggttt gaattgatgc catcgacggg atcaggttca gttttttaag
     5641 ctgggcaagc gggaagggac catgatcaac cgattcgttg aagatgacgc tctgaccgct
     5701 tttaatccac ggattttctt tcccggcaat gggtttcacc aacagttgca actggctgct
     5761 gaatacgccg cgatgatagt tttgataact cacttccagg ttggattcag gagctgtcag
     5821 tttgagttgc gcgttcgcct gcgcgaccat gtcttcgaga tgggtttcaa tcttcttgcc
     5881 tgtataccat gcgccgcctg tccagactac gcctagcgca acaatgacgc ctaccgctac
     5941 cagcgattta ttcataatga ttatccataa aatgaaatca ggcggactgg ccgcctgaag
     6001 gtgttataag cctttaataa gcttagcaag agatgttaat tttttcagta agctcttaca
     6061 gcttgttgta aacacgcgct aaacggccgt ggcctttgac agtcaccggt gattcgttgg
     6121 cggcaataaa cgctgattca cccggtttaa gctgtaactg ctgagaacct ttccacaacg
     6181 ttgcatcgcc ttcgacgcag aacaaaatgg cggcactctg ctggctgatg gtggtttctt
     6241 tatcactaag gtcatgcagc gagaaggcaa aatcatccac aggaatcggg aagtccagtt
     6301 ctgcaccttg tttcaccggc tgggtcaaca actggttagc cggtttggct tcgaatttca
     6361 cattggcaac cagttccgga atatcaatgt atttaggcgt cagacctgca cgcagcacgt
     6421 tatcggagtt cgccatcact tccagcgcca cgccttgcag gtaagcgtgc ggtgtttcag
     6481 cgaacaggaa catcgcttcg ccaggattca atttcaccac attcagtaac agcggggaga
     6541 acaggccgct gtcttccggg taaaattcag aaattaaacg aatcgtttgc cacggttcac
     6601 cctgctgact gtcgagcgcc gattttaaaa tcgccagcgc gcgggatttt tcttcaccct
     6661 gcatattcaa caggttggcg aacagttcgc ttaaacgttc ggcatgtggc tgttgtaaaa
     6721 agtgagcaat cgccggatgt gcaccggcga ccggctggag tagggagaca atctcggaaa
     6781 attcacgaaa cgcgttcatc gcaaggaaag gcgtcagcgc aaaaaccagc tccggcttgt
     6841 ggttaggatc tttatagtta cgctcggcgg catccatcgg gatacctgcg gcattttctt
     6901 tggcaaaacc gatttcagaa ttgtgtttgt ttggatgaac ctgaatggag agtggctgtg
     6961 ctgcgcataa tactttgaac aggaaaggca gttcgccaaa gcgtttggca acggcctctc
     7021 cgagcagagt cgatttatca ctctcaatca catcacgcag tgaaacgata tctccggcgg
     7081 cattctgcac tcgtgaactg cttttcggat gtgcgcccat ccacagctcg gccatcggct
     7141 ggctggacgg attttccata ccataaagtt cagtcaacgc cgttttgcta ccccaggcat
     7201 agttttgcac tgagttaatg agtttttgca tgatcaatcc ctgttttaat gtggaaatta
     7261 atcccgctat taaagcaaga atcctacggg aagtaacctg gagccgcaaa aagtcgtact
     7321 agtctcagtt tttgttaaaa aactgtgtag gatattgtta ctcgctttta acagggcaac
     7381 ggaacacccg cccagagcat aaccaaacca ggcagtaagt gagagaacaa tgtcaaacaa
     7441 accctttcat tatcagtctc cttttccact caaaaaaaat gatactgagt attacctgct
     7501 aaccagtgaa cacgttagcg tatctgaatt tgaagggcag gagattttga aagtcgcacc
     7561 cgaagcgtta actctgttgg cgcgtcaggc gtttcatgat gcgtcgttta tgctgcgtcc
     7621 ggctcaccaa caacaggtgg ccgacattct gcgtgacccg gaggccagcg aaaatgataa
     7681 atatgtggcg ctgcaattcc tgcgtaactc cgacatcgcg gcgaaaggcg ttctgccaac
     7741 ctgtcaggat accggcaccg cgattattgt tggtaaaaaa gggcagcgtg tatggaccgg
     7801 tggtggtgat gaagcggcgc tggcgcgcgg tgtctataac acttatatcg aagataatct
     7861 gcgctactcg caaaacgcgc cgctggatat gtataaagaa gtgaataccg gcaccaatct
     7921 gccagcgcag atcgatcttt atgccgttga tggcgacgaa tacaaattcc tctgtatcgc
     7981 aaaaggtggt ggttcggcaa acaagacgta tctctatcag gaaaccaaag cgttactgac
     8041 gccggggaaa ctgaaaaatt acctggttga gaagatgcgc acgctgggta ccgcggcctg
     8101 tcctccgtat catattgcgt tcgttattgg tggaacttct gcagaaacga accttaaaac
     8161 ggtgaaactg gcttccgcga aatactatga tgaactgcca acggaaggga atgagcacgg
     8221 tcaggcgttc cgcgatgtgg aactggaaaa agaattactg atcgaagcgc aaaatctcgg
     8281 tctgggtgcg cagtttggtg gtaaatactt cgctcacgac atccgcgtga ttcgcctgcc
     8341 acgtcacggc gcatcctgtc cggttggtat gggtgtttcc tgctctgctg accgtaatat
     8401 caaagcgaag atcaaccgtc aggggatctg gatcgaaaaa ctggaacata atccaggtaa
     8461 atatatccct gaagagctgc gtaaagcggg agaaggcgaa gcggtgcgcg ttgaccttaa
     8521 ccgtccgatg aaagagatcc tcgcacagtt gtcgcagtat cccgtttcta cacgcttatc
     8581 gcttaacggc acgattatcg ttggtcgtga tattgctcac gccaaactga aagagcggat
     8641 ggataacggt gaagggctgc cgcaatacat taaagatcat ccgatttact acgcgggtcc
     8701 ggccaaaacg ccggaaggtt atgcctccgg ttctcttggc ccaacgaccg ccggacggat
     8761 ggattcttat gtcgatcaac tgcaagcgca gggcggaagt atgatcatgc tggcgaaagg
     8821 caaccgcagc cagcaggtga cggatgcctg taaaaaacac ggcggcttct accttggcag
     8881 tatcggtggt ccggccgctg tattggcgca gggaagtatt aagagcctgg aatgtgttga
     8941 atatccggaa ctgggaatgg aagccatctg gaaaattgaa gtggaagatt tcccggcgtt
     9001 tatccttgtg gatgataaag gaaatgactt cttccagcag atacaactca cacagtgcac
     9061 tcgctgtgtg aaataaacag agccgccctt cggggcggtt tttttacatg gcacgaaaga
     9121 ccaaacattt gttatcaaat agtaaataat gagtgagcta aaagttgctt aacgaaagca
     9181 aaacagaaag aaaaaattaa tcaggtgagg agcaggccat gaatacagta cgcagcgaaa
     9241 aagattcgat gggggcgatt gatgtcccgg cagataagct gtggggcgca caaactcaac
     9301 gctcgctgga gcatttccgc atttcgacgg agaaaatgcc cacctcactg attcatgcgc
     9361 tggcgctaac caagcgcgca gcggcaaaag ttaatgaaga tttagacttg ttgtctgaag
     9421 agaaagcgag cgccattcgg caggcggcgg atgaagtact ggcaggacag catgacgacg
     9481 aattcccgct ggctatctgg cagaccggct ccggcacgca aagtaacatg aacatgaacg
     9541 aagtgctggc taaccgggcc agtgaattac tcggcggtgt gcgcgggatg gaacgtaaag
     9601 ttcaccctaa cgacgacgtg aacaaaagcc aaagttccaa cgatgtcttt ccgacggcga
     9661 tgcacgttgc ggcgctgctg gcactgcgca agcaactcat tccgcagctt aaaaccctga
     9721 cacagacgct gagtgaaaaa tcgcgtgcat ttgccgatat cgtcaaaatc ggtcgaaccc
     9781 acttgcagga cgccacgccg ctaacactag ggcaggagat ttccggctgg gtagcgatgc
     9841 tcgagcataa tctcaaacat atcgaataca gcctgcctca cgtagcggaa ctggctcttg
     9901 gcggtacagc ggtgggtact ggactaaata cccatccgga gtatgcgcgt cgcgtagcag
     9961 atgaactggc agtcattacc tgtgcaccgt ttgttaccgc gccgaacaaa tttgaagcgc
    10021 ttgcgacctg tgatgccctg gttcaggcgc acggcgcgtt gaaagggttg gctgcgtcac
    10081 tgatgaaaat cgccaatgat gtccgctggc tggcctctgg cccgcgctgc ggaattggtg
    10141 aaatctcaat cccggaaaat gagccgggca gctcaatcat gccggggaaa gtgaacccaa
    10201 cccagtgtga agcattaacc atgctctgct gtcaggtaat ggggaacgac gtggcgatca
    10261 acatgggtgg cgcttccggt aactttgaac tgaacgtctt ccgtccgatg gtgatccata
    10321 atttcctgca atcggtgcgc ttgctggcag atggcatgga aagttttaac aaacactgcg
    10381 cagtgggtat tgaaccgaat cgtgagcgaa tcaatcaatt actcaatgaa tcgctgatgc
    10441 tggtgactgc gcttaacacc cacattggtt atgacaaagc cgcggagatc gccaaaaaag
    10501 cgcataaaga agggctgacc ttaaaagctg cggcccttgc gctggggtat cttagcgaag
    10561 ccgagtttga cagctgggta cggccagaac agatggtcgg cagtatgaaa gccgggcgtt
    10621 aatctgcaac atacaggtgc agccgtggaa tgatcaaacg aagcggctgc gcctgaggtt
    10681 tataacggtg ctgcacattg tcggcatcgt aatttaacaa ctcaccaacg tccggcacgc
    10741 ccgcgccatt atcccgatta atcagtgcaa tcagtggtgt agggcaggcg tgttggactt
    10801 gtttttgatc tcctttgtac cagacgcggg caatcggctg caccttcact ggacgtttga
    10861 tttttaactt cgcgttctgt ggcagggcag cgatatcctg atactctcgc tccagttttc
    10921 tttgccactc ctcgcgcgtc cacggtgcga cactgcgtgg tgatttcagg cttttttcca
    10981 gctgtgccag gacttcatca cgatgcaaat tcttaatgat atgtttatta gcccaaccaa
    11041 agcgtaaagt ggcggggtcg tgcagaacgg tgagcgtgcg gtaagcatta agggtgatca
    11101 gccccggcaa atgacgatgc acccattcaa aacgtgccgc ggtggggagt tctgattcaa
    11161 ccgtgacgat atgctcgaac gtggtcttga gtttattgat gtgctgaata tgactgacca
    11221 acgctgcttg cgaaaggtta tcgacctggt aacacaacac gccaggcaga cggacagcgg
    11281 ccttgctgct gcgattttcg gactgttgtt gaataaataa atggcggaaa tgacgcaacg
    11341 ccagcgactg cgcgtcgttg ccgagatgtt gttttacctc aatacgatta agcggattat
    11401 gctcatcctc tttttttacc tccggcaaag agaacacgcg ggcaaccaat agcttgtgtt
    11461 gctcaagatg agcggcaaat gtagccagct cttgttccat ctggcgaaag gtagtgttga
    11521 gtcggtctac gagatcgtaa cgcgccataa tattaaccac tttagttaca acatacttat
    11581 tttatagcac agtcgtggtg acttgaccat cgtgcgcgta gtgtcaggca gaggtaaatt
    11641 gcgggatgtt atgccataac ggccagctaa acgagaagcg agcaccaccc agttcgctga
    11701 tgtcacaatt aaccgtaccg cccattgtca gtgctattga gtggacaatt gccagcccca
    11761 gcccgcagcc gccggttgag cgatcccggc tgggatcgag gcgaacaaaa ggttcaaaga
    11821 tatgttcgcg gttttctggg gcaatccctg ggccatcatc ctcaacaatt aatgtcgctc
    11881 tattccccga cagtagcagg ctggtttcaa ccgttgaatg gcagtagcgc agggcgttat
    11941 tgagcaaatt atccagcacg cgctccatta agcgcatatc caacgccgca taatggcctt
    12001 gcatgagcgt ttttatccgt accgttttat cgggcgttac tgcctgaata tctgccagat
    12061 gcgttgacag ccacaacggc aggtctggtt cgctaagatg aagctcgttt tgtgggcgat
    12121 cgagtcgggc ataagtcagc agctcttcaa ttaaagcttc aagttgactg atatcacgat
    12181 tcaacgcctg ggattcggcg gcgctcaggt tatcgctcat ctccagtcga taacgcaggc
    12241 gcactaacgg tgttcgcagt tcgtgagcga taccgtcaat aagctgtttt ttgctggcaa
    12301 ttaaggcgtt gatattgtcc gccatctggt taaatgcgat accaagtcgt tcaaagctcg
    12361 aaccctcatc aaagtggata cgttcattga gatgcccatc gccaaatcgt tgcgccgctg
    12421 cttccagttt taacatatcc tgccagtgcg gacgcatcca gataaacacc ggaaaggcga
    12481 gggaaatagc aataaaagcg atcagggcga tatccagcaa tcgcatttga tggaggtaat
    12541 aaagataagg aacaggacca actgccagca cgtagtggct gcgcgggata cgctgcaaaa
    12601 acgtgtactg atcgtccagg gcgacaattt cgccgccacg cagtcggtgc atggaaatat
    12661 catcaagatg gtatttactc agtggctcga cacgcagatc gaaagagaga tttaaatcca
    12721 tctctttcag cgtcttaccc cagtcgtgtg gggggatctc tcgcaattcg ctgcgcatca
    12781 gatacagcga actgttcatc aaatcatcca gcgactgttt gcccgcgcgt tcggcggtaa
    12841 atttgtacac cagcccaacc agcagagaca tcacaaggaa gcagacaaac aataacaggt
    12901 aaaactggat aaacaatttt ttcattggca tcgcccgcaa cagaatacta ttcgtattaa
    12961 ttcacgactg tggagcaatg taaaccgcca actggataag accttgtttg gaagataatg
    13021 tccggaacag acggatgtat ccccggtgta ttcatattca gtaaattcaa ttttccaccg
    13081 gggcttgttt attatgctaa ggtttaattt aacgtgacgc tttgttgttc tttagtctct
    13141 ctatccttaa gaagacgggc tgatattaca ccggacatta ttgaaccatt cacattaagc
    13201 gctgttcttg ccatatcaac gatcggctct atggcaataa aaataactac taatgcgaca
    13261 gggaaattaa gtgttgaaag gacaatcagc gccgcgaatg tcccaccact acctacgcct
    13321 gctaccccta ttgagccaag cgcgatggca ggtaaaagag ttgcgaggaa ttgcaatgag
    13381 aagggatcga tacccattgt tggagcaatc atcgccacca tcacggcagg ataaatccca
    13441 gcgcagccat tttgtcccat gctggaacca aaagacgctg caatattagc aatcgtattt
    13501 ggaaccccca atttttccaa tgcagaaata gccagaggaa ttgaagccgc actactgcga
    13561 gaaacaaacg caaaggtaag tacttgccag acggtcttgt aatagttaac ggggttttta
    13621 cctattaaca gcaaaatgcc cgagtgaaca ataaacatca caaaaacggc cagataacag
    13681 gcgacgatga acccgagcaa attggtaaac tgtgaaagct gatacgttga gaagactttc
    13741 gccattagtg ccattacccc ataaggcgtc agcgcaatga caatacggac cattttcatt
    13801 acccatattt gaatcgcgtt gatccccgcg ctcaattttt cgccctctgc tggtgcatct
    13861 ttctttactt ttaacagcgc aataccagca agaattgtaa agatgacgat gccaataacc
    13921 gaaacacttc ttgccccggt cagatcaagg aagaggttgg ttgggatcaa tgacgtaatg
    13981 agtcgcggta aagataccgc agctgttttc gagacgtcat cgctggttaa agaggattgc
    14041 atatgaccaa aggcgctggc atcaaggcca attgcactgg caatagccag gccaactaaa
    14101 cctgcaatca tgactaaaca caacatacag gcaacaatag ttaaagacat cttgccaata
    14161 ccagcgctat tttcgagctt attaatcgct gaaagaatcg aaatgaatat gagtggaacg
    14221 gcaacaagtt ttaataaatt aacataaccg ttaccagcaa tattatacca ttcgataacg
    14281 gtttcactgc ttgtctcagg aatgaaatgc agggagatac caatgatagc ccccaggaaa
    14341 atcgcgataa aaacactttt ggaaagggat tcgctctttt gatccgtttt gaaaatcccc
    14401 catgagcaga tcaaaaaaag cacaatatat gtgaaagttg taaccgtttt ttaagcctct
    14461 tgcctcattc agcacctgaa atgccggaaa gagtttgttg taaaaaatct gcgccataag
    14521 acgcagggac gaggcgatta tagagaaatt ttttctcatt ttcatatcaa atgtaggggg
    14581 tttggaattt gaataagctc ttattaattg aattcgttaa ttgttctctt tttcagatga
    14641 acaaatatta gtcatctgat tgtttattgt cactatccca tgcatgaggc gcaaaaagat
    14701 agcctttgtt acgcacagtt ttaatgcgat aaggttctgc ggcgttatcg agcagttttt
    14761 ttcttaaccg cgaaatagcc acgtccacgc tacgatccag tccgtcataa ctgacgccgc
    14821 gtaaattttt cagcaatgca tcgcggtcca tgatttgccc ggcatgcgta gctagttccc
    14881 acaataattc gaaatcagct gtcgagagcg agatttcagt gttagccagg gtgactacgc
    14941 ggttgatggg gtcgatagtt aacgtgccga aatgcagggc tttgtaggga gtaagagacg
    15001 tttcctgaag acctttggtc agtgtggctt gctcattctg acgcaaatgc aaacgtaaac
    15061 gcgctagcaa aacagcaggg ggcgtcgttt tgagaatata gtcgcaggca cccatttcca
    15121 gtgccaggat gtggttcata tcgctatcga gagaggttag aagaacaatc ggtccagacc
    15181 actttgcgcg taaatcacga caaatggtca tgccgtcctt gcctggtagc atgatgtcga
    15241 gtaacaccaa atccggattt tctcgcaaaa tggtttcttc ggcctggtcg ccgcgcggtt
    15301 ctacggtaac ctgcatatca tgttttgcca ggtacgcggc aatcagtgaa ccgacttccg
    15361 catcatcttc cacaaatacg atagtgttca taacattcac ggtagatata aaaacgtcac
    15421 aatacaccgc gctgttttta cttactatca atcgtttcta aacaagtttt catcaggtat
    15481 tctactgatg acttgttatg gaagtgttaa ggtaaaaaga tggggctggt aatcaaagcc
    15541 gcgctcggtg cgctggtagt gctgttgatt ggtgttttag caaaaacgaa aaattattat
    15601 atcgccgggc tgattccact tttcccgacc tttgcgctta tcgcgcatta tattgttgcc
    15661 agcgaacgcg gcattgaagc cttacgcgca accatcattt ttagtatgtg gtcgattatt
    15721 ccctattttg tttacctggt gtcgctgtgg tatttcaccg gaatgatgcg cttgcccgcc
    15781 gcttttgttg gttctgttgc gtgttggggg ataagcgcat gggtgttgat tatatgctgg
    15841 ataaagctgc attaacgcag atgacgaccg ccatcaagtg ggaaactgcg tccggtgaca
    15901 aagcaactgg taagtaagta atcgaccagg tcgatcactt ctttctcgcc aggcgcggtt
    15961 ttcatcagtg atttattcag cgcctgttgt cgatattcgg catcatcatg ttcattaaac
    16021 aggatcagcg atggcgcaat agaattcact ttcacttccg gtgccagctt gcgggcaaac
    16081 gagcgggtca tattatccag tgccgctttg cttgcagcat acgcaacatg tttgtcgcta
    16141 ccgcgctcca ccacataatc ggtaaagtga atgatatcgc tggcggcgtg tccgtgccca
    16201 cgcagtaatc tttccagcgc atggttgagc aggtatgggg tattaacgtg gatctgcatc
    16261 atgcaagcca gtacgtcggc cagtggcgca cccggttttt ccgcaatcca cgcactggcg
    16321 ttatgcaaaa tagcacgcag accatgggtg gtttttagta cttcatcggc aaacgccatc
    16381 acaccgtcgt tggtcgaaaa atcagcctga atacactgcg cacctgcatt aatcagtcca
    16441 tcaatggctg gatagtgtgt ccgatagctg acaatcaccg gttgcttttg attaatgaaa
    16501 tgccatgcga gggcgaggcc gatgcgacga cctccgccag taattaatat tggcaagggc
    16561 tgggttttac ccatcgttat ctcctttgct atccaacgat gggatgaacg ttatcccacc
    16621 agcatccagg tcgccggaac tgaggcaatt aacagcatgc caatcagcac catttcctga
    16681 cggttcagta cattatcatg tgtatgcgtt ttgcgcgcat acaggaaaac cagcagcccc
    16741 ggtgcataca gaacaacgga taacagcaag tgcattggac cagaggcgta caataaccat
    16801 aagccataaa tgcaggcacc gacacctacc gctttatgca gtggacgggt tgcgattttc
    16861 agcaagaacg cgccgaccag gaaatagggc accagaatca tttctgaggc gatggtcagc
    16921 aacgtgttgt aatcggaacc ggtcagccag atgagtacca gacaaatttg cacgcagatg
    16981 ttggtcaacc acagtgaggc ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgt
    17041 gggaatgctt tatgagtggc agccaggaac ggaacttctg ccgccatgat ggtccagctt
    17101 aagtacgcgc cgcaaacgga aacgatcaaa ccggcagcga tgatgatttc gccccatggt
    17161 cccatcattt ccaccatcag accggccatt gacgggttac gaatttcagc cagttcagga
    17221 cgtgccacca cacccagcga aagcagcgtt accagcaagt aaacgcccag agcggagaga
    17281 accgccagca gtgtcgcttt gccaacatca cgtttattac gcgcacgcgc agaaacaacc
    17341 acggcacctt ccacaccaat gaaaacccac agggtgatca gcatggtgtt tttcacctgt
    17401 tcccaaacag gtacgccaag tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg
    17461 aacatcatca tcgccagcac aacaaacaga cccagcggca acaattttgc cagtgtcgcc
    17521 accaggttaa tgctggcagc ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac
    17581 aataaagccg atgcaccgac tatcgattgc caggtattac catcgccaaa caggcgcaat
    17641 tccggcgtgt ccgtaaaaaa gcttaacgcg gaaaaaacga tcaccagata ggagacgttg
    17701 gcgatgaccg cgcacagcca gtatccccat gcggaacaaa agccgattag ctcgccaaac
    17761 ccttcgcggg cataggtaaa gataccgcca tcaagttcgg gacgaatgcg cgtgaggatc
    17821 agcatggcaa aggccagcaa taaaatgcca gcgccagtaa taccccagcc gatgagcagt
    17881 gctgccgggc tggcaactgc cgccatattt tgcggcagac tgaaaacacc cgcgcccagc
    17941 attgagctta ataccagcgc ggtgagtgcg ctcagtccca gtttcttttc catcgatatc
    18001 cagtgattaa aacgtgataa gaagaataaa tattagggca aaacgcataa aaatggagtt
    18061 tgccactgaa gcgcgggatt ttacgaagag atgtgtctgc atgcaatggt cgcggggaaa
    18121 tttgtgacta aaaatgcgaa aggcggcatc aagccgcctt ataggagtaa ctgaatagtt
    18181 atttatacag atctgcgctg acggtcaggt tattaccacg tttctgccac tggcgggtga
    18241 tgtggtaata cttagcacct ttcttcgcgg cacgtttcgc aacctgatag gagacttcgg
    18301 tcatgttgcc gtagttgcca gagaatttga tgctgtcgaa cgggaccatc atcgctgctg
    18361 tcgctttgtt cagttcttcg actttagtgc catccgggag cgtgacggtg taacgcccgc
    18421 cttttgatga ctgggtttca aagaagcgac cgacttcaga acttggtgat gcggtagtcg
    18481 caacacccgg gatctcaact ttcttcgcgg cttcgccacc ggcagccaga gctgcacgtc
    18541 ctgcttcgga atctgccggg atcacatccg ggctctggac gatacgtttc ttagcatctt
    18601 ttttatagat gaatgcagta atacgctggt tgccgccctg gttggcatcg atttgacgaa
    18661 cgatgtagaa agagtaggca cctttcgctt ttgccgcttt ggtgatggca tcattgactt
    18721 ctggctggct acgatagaag ccctggacgg tgaccgtgtc aaacggttca atcagaacag
    18781 cctgatcttt cggcagttcg acaacaccgt taattacgcg attacttgtt tcttctgctt
    18841 tttcagcatc ggctttatag aggtcagcga ccacacgcca gttaccgctg ttaccaaaat
    18901 cagaagtgtc gacaacataa aaagaggcgg caccttcttt atctgcgcga cgagaaacgg
    18961 ctttcaccgc ttcgccaata gcattaaaac gaccggtaac cactacacgg tcaaaaggtt
    19021 taaccgctgc cgcttgctcc ggtgtcagtt ctgttgctgc gttaacggag aatgccgtag
    19081 cagaaagcag tgccgacgcc aggagggtgt tcttaagctt cataaaaata atccttcgcc
    19141 ttgcgcaaac caggtactgg tattgttatt aacgagaaac gtggctgatt attgcattta
    19201 aacggtgtaa ctgtctgcgt catttttcat atcacattcc ttaaaccaat tttaatcctg
    19261 ctcaaatgac cgtctatgct taaaaaacag ccgtatcagc atcattacta ctgaagcaac
    19321 tgaattgtat aagttaattt aatgttaagt agtgattcgt gccggggcga tgtctcgttt
    19381 tacccgaccg tcgaagacaa ttatcagtct ttatccggcg ttctaaggtg tttatcccac
    19441 tatcacggct gaatcgttaa tattttgcga gttcacgccg aaatactgat ttttggcgct
    19501 agatcacagg cataattttc agtacgttat agggcgtttg ttactaattt attttaacgg
    19561 agtaacattt agctcgtaca tgagcagctt gtgtggctcc tgacacaggc aaaccatcat
    19621 caataaaacc gatggaaggg aatatcatgc gaattggcat accaagagaa cggttaacca
    19681 atgaaacccg tgttgcagca acgccaaaaa cagtggaaca gctgctgaaa ctgggtttta
    19741 ccgtcgcggt agagagcggc gcgggtcaac tggcaagttt tgacgataaa gcgtttgtgc
    19801 aagcgggcgc tgaaattgta gaagggaata gcgtctggca gtcagagatc attctgaagg
    19861 tcaatgcgcc gttagatgat gaaattgcgt tactgaatcc cgggacaacg ctggtgagtt
    19921 ttatctggcc tgcgcagaat ccggaattaa tgcaaaaact tgcggaacgt aacgtgaccg
    19981 tgatggcgat ggactctgtg ccgcgtatct cacgcgcaca atcgctggac gcactaagct
    20041 cgatggcgaa tatcgccggt tatcgcgcca ttgttgaagc ggcacatgaa tttgggcgct
    20101 tctttaccgg gcaaatcact gcggccggga aagtgccacc ggcaaaagtg atggtgattg
    20161 gtgccggtgt tgcaggtttg gccgccattg gcgcagcaaa cagtctcggc gcgattgtgc
    20221 gtgcattcga cacccgcccg gaagtgaaag aacaagttca aagtatgggc gcggaattcc
    20281 tcgagctgga ttttaaagag gaagcgggca gcggcgatgg ctatgccaaa gtgatgtcgg
    20341 acgcgttcat caaagcggaa atggaactct ttgccgccca ggcaaaagag gtcgatatca
    20401 ttgtcaccac cgcgcttatt ccaggcaaac cagcgccgaa gctaattacc cgtgaaatgg
    20461 ttgactccat gaaggcgggc agtgtgattg tcgacctggc agcccaaaac ggtggcaact
    20521 gtgaatacat cgtgccgggt gaaatcttca gtacggaaaa tggtgtcaaa gtgattggtt
    20581 ataccgatct tccgggccgt ctgccgacgc aatcctcaca gctttacggt actaacctcg
    20641 ttaatctgct gaaactgttg tgcaaagaga aagacggcaa tatcactgtt gattttgatg
    20701 atgtggtgat tcgcggcgtg accgtgatcc gtgcgggcga aattacctgg ccggcaccgc
    20761 cgattcaggt atcagctcag ccgcaggcgg cacaaaaagc ggcaccggaa gtgaaaactg
    20821 aggaaaaatg tgcctgctca ccgtggcgta aatacgcgtt gatggcgctg gcaatcatcc
    20881 tgttcggttg gctgtcaagc gttgcgccga aagagtttct tgggcacttc accgttttcg
    20941 cgctggcctg cgttgtcggt tattacgtgg tgtggaatgt atcgcacgcg ctgcatacac
    21001 cgttgatgtc ggttaccaac gcgatttcag ggattattgt tgtcggagca ctgttgcaga
    21061 ttggccaggg aggctgggtt agcttcctta gttttatcgc ggtgcttata gccagcatta
    21121 atattttcgg tggcttcacc gtgactcagc gcatgctgaa aatgttccgc aaaaattaag
    21181 gggtaacata tgtctggagg attagttaca gctgcataca ttgttgccgc gatcctgttt
    21241 atcttcagtc tggccggtct ttcgaaacat gaaacgtctc gccagggtaa caacttcggt
    21301 atcgccggga tggcgattgc gttaatcgca accatttttg gaccggatac gggtaatgtt
    21361 ggctggatct tgctggcgat ggtcattggt ggggcaattg gtatccggct ggcgaagaaa
    21421 gttgaaatga ccgaaatgcc agaactggtg gcgatcctgc acagcttcgt gggtctggcg
    21481 gcagtgctgg ttggctttaa cagttatctg catcatgacg cgggaatggc accgattctg
    21541 gtcaatattc acctgacgga agtgttcctc ggtatcttca tcggggcggt aacgttcacg
    21601 ggctcggtgg tggcgttcgg caaactgtgt ggcaagattt cgtctaaacc gttgatgctg
    21661 ccaaaccgtc acaaaatgaa cctggcggct ctggtcgttt ccttcttgct gctgattgta
    21721 tttgttcgca cggacagcgt cggcctgcaa gtgctggcat tgctgataat gaccgccatt
    21781 gcgctggtat tcggctggca tttagtcgcc tccatcggtg gtgcagatat gccggtggtg
    21841 gtgtcgatgc tgaactcgta ctccggctgg gcggctgcgg ctgcgggctt tatgctcagc
    21901 aacgacctgc tgattgtgac cggtgcgctg gtcggttctt cgggggctat cctttcttac
    21961 attatgtgta aggcgatgaa ccgttccttt atcagcgtta ttgcgggtgg tttcggcacc
    22021 gacggctctt ctactggcga tgatcaggaa gtgggtgagc accgcgaaat caccgcagaa
    22081 gagacagcgg aactgctgaa aaactcccat tcagtgatca ttactccggg gtacggcatg
    22141 gcagtcgcgc aggcgcaata tcctgtcgct gaaattaccg agaaactccg cgctcgtggt
    22201 attaatgtgc gtttcggtat ccacccggtt gcggggcgtt tgcctggaca tatgaacgta
    22261 ttgctggctg aagcaaaagt accgtatgac atcgtgctgg aaatggacga gatcaacgat
    22321 gactttgctg ataccgatac cgtactggtg attggtgcta acgatacggt taacccggcg
    22381 gcgcaggatg atccgaagag tccgattgct ggtatgcctg tgctggaagt gtggaaagcg
    22441 cagaacgtga ttgtctttaa acgttcgatg aacactggct atgctggtgt gcaaaacccg
    22501 ctgttcttca aggaaaacac ccacatgctg tttggtgacg ccaaagccag cgtggatgca
    22561 atcctgaaag ctctgtaacc ctgacggcct ctgcggaggc cgtcactctt tattgagatc
    22621 gcttaacaga acggcgatgc tttgacctcc cgctgtttgt tcaagcgcaa ttttgacaat
    22681 aattgtcaac ggcacggaaa gcagcatacc caccggtcct aacaaccatc cccaaaaaat
    22741 caacgacaaa aataccacca atgtcgaaag ccccagccca cgtcccatga tgcgcggctc
    22801 cagaatattg ccgaagacca gattaatcag cagatatccc gccagcacca gcaacgcttc
    22861 gtagaagcca ttaaacacca gtacctgagc gatagggggg attgccgcga gaactgaacc
    22921 aatattaggg atgtaattaa gcgcaaaggc cagcaatccc cagacaaaag cgaagcgaac
    22981 atcgagtgcg gcgagcatcg cccaggcgac caggccggtg atgatgctga tggctgtttt
    23041 cagcaccaga taatgagaaa cactgtcaat cgcacgttga atcgccgcca tcccttcaac
    23101 cggacgcgcc atcatttgct gaaattttcc gggcaattgt ggcacttcga gcagcataaa
    23161 cagcaccgtc agcagcaata aaaatattga tgacatggca ttagataact gcgtcaataa
    23221 gttggtgagc aacgtcatcg ccgcgttcgg atcaatgtaa tgcgccagtt ggtcaactga
    23281 gacgtcaatc cctacgcgtt gcaacaacgg ctcaagagct tgcagcggcg tcataataga
    23341 gttgcgatat tgcggtaacg tccgcgtcaa ctcgttgagc gcggaaccca gataagctaa
    23401 tagcaacacc atcgccatca cgatgattgt catcaaaatc gacactgcca gtacacgcgg
    23461 cacacgccag cggaccatat gttgcaccag cgggttaaga ataacagcaa taaataatgc
    23521 gagaataaac ggcacgatga tctcggcggc aaaacggata ccgcagagaa taatgaccag
    23581 cattcccaac ataatgacga tttttaggcc attgagcgtg atgatcggct ttgccatgct
    23641 tgtccccgtt tttcttcctt tgctaataat aatgtctttt tcgctccgtc atcataacga
    23701 aaatcaaacc gctcaggtaa agaagtgaaa atattttgag ttaattctta agctatgata
    23761 caaatcaggc gtgttcaact accgaggaca attatcatct gcgatgacga gaagcaacac
    23821 tgcggataat tgtaatatta tggacaattt gttcaggacg ttattttcta catttaccca
    23881 tcttcgtacc tcatccacta tcttgctcgt aggtgagcag cactggcgca acgcgcttta
    23941 gtcctttctt ctgcctaagc ttgccctatg cgcagggctt gaccacttta ctttggtttc
    24001 gctgaattaa gcgaaaatta aaatagttct cttgcaggag aaggacaatg tatatttatt
    24061 ggattttatt aggtctggct attgctacag aaattaccgg tacgctgtca atgaaatggg
    24121 cgagcgtcag tgagggaaat ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt
    24181 atatatttct ctctttcgcc gttaaaaaaa tcgccttagg cgtagcttat gcgctgtggg
    24241 aaggtatcgg tattttattt attaccttgt ttagcgtttt gttattcgac gaaagtttat
    24301 cgctgatgaa aattgccggg ttaaccaccc tggtcgccgg gatagtgttg atcaaatcag
    24361 gtacccgtaa agcgcgtaaa cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt
    24421 cacgccgcct ggctggcatt ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa
    24481 ttttctgacg gctttcgtcg caaaatattt ggcttgctct ccctggcggc ggtgctggcg
    24541 gcctttagtg cgctttctca ggccgttaaa gggatcgact tgtctgtcgt ttatgcattg
    24601 tggggcgggt ttggtattgc cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg
    24661 ttaaatcgta aaggctggat tggcctggtc ttgctgttgg ctggaatgat catggtgaaa
    24721 cttgcctgat gaagacgctg cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac
    24781 agttgatcca gcttgtcgcg aaaaccggta acagaaatgg cccggttatc ggcgcgccag
    24841 cgatctttcg cggcaggagc cgaactttgc accccaatta attgccagcc gtcatcggta
    24901 tgcaacatca gaggcgaacc gctgtcaccc ggcaaggtat cgcactgatg tgacatcacc
    24961 gacgtttgcg cccagccagt cacttcacag ttttgatgac tgtataacgt atcgagatga
    25021 tcttcagggt atcccgcctg agtcacttta cgaccagccg attttaatgc ggcagtaagc
    25081 gcggctttat ctccctcaaa taacggcaac ggtgtaatgc cagaaggggg attacgtagc
    25141 acaatcaatc cgaagtccca cggtgcagct gcgggaggaa caatccaacc atccccatct
    25201 gcttttaacc gctttcccag tgtcggatca acgcggcctt ctatgtcgtg gatctcatag
    25261 cgccaaagac ctttatttga cacaaaacgc agcgctaccg ccttatccgc tttaccctta
    25321 ggcggcgtca ataaacagtg tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc
    25381 gtacataaat tgccgctggc cgtttccagt tgcccaaccg catcccacgg tgattgggtc
    25441 gtgtcattca ctggcacacg atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt
    25501 gccgatttgg caacgtctgg tttatctgca aacacaaaag cagacgtcaa actaattgca
    25561 cccaacacta cagcaatggt tgtacgcata tcacactctg gtgggtaatt atgattatta
    25621 aaagcaaacc ctcataaata ctatagacgg gacgggatga aagtgggagt aaaatcagat
    25681 aactacagtc aggaaagata aaaacgggcg gcaatcagtg cgcataaaat aaggatgata
    25741 agaatgaact caaagcgata acggcccacc atactaccct ccgctaaagg cggtgctaaa
    25801 cgttacccgc ttagcaccgt aattttcagg cgcgaggggg cgcgccagca ttactgttga
    25861 aaacttacgc tgcgggttgt gcagcaggtt ttgccggttg ctgatggccg tgtttcccgg
    25921 cgtgtttctt cgctgcctgc gctttttgtt caggggcttt ctgttcagct ttcgtatttt
    25981 tatgatgctt tttagccgcc tgcgctttct gggcaggtgc tgctttatgc tgttttttat
    26041 gatgtgtagt tttcgctggc gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg
    26101 cagtctctgc agcaaaggcg gcagaagaca gacccatagc agcggcaaca accagagcta
    26161 atactttttt cattgtcata ccctcaattt gttttttcat ttaaccccac tgcggggccg
    26221 ttgaaataac tatatccctg ggaatggcag acttccgtga gtggttggtt tcagcgtgta
    26281 acgatatgta caaacgctga ataaattaca gcgttgataa taacgtattg tgacttaagg
    26341 aaaatttagc taccaataat agtagtcttg atcgggttaa ctattatact acttacaaag
    26401 cggtagaata actgcgcatc agtaataaat gacacacagc aaaatgaatc cgtttatttg
    26461 ggtacttata atcctgatga cgctagatgc gctgcgggaa ttggctggcg cttcgtctat
    26521 tttaggatgg ctattaacgt tggtttgagc aggcaataag tccggacggg tatttaccgc
    26581 agtccggact tatttttcag gcgtgcagac gacgatgcaa acgcgtcccg accagcagag
    26641 caatgaccag catcagagca ataaatgcgc cgacgccgtt ccagccatag ttatgccaga
    26701 aaacaccacc cagcgtcccg gcaatactcg accccagata gtaactgaac agatacagcg
    26761 aggaggcctg gcctttagcg cgttttgcgc gggggccgat ccagctgctg gctactgagt
    26821 gggctgcgaa gaatccggct gagaagagta acattccggc aaagatcagc cacagcgagc
    26881 taaataaggt catcagtaaa ccaaacggca taactcccgt cgaaaacaac atcaccggac
    26941 cgcgcccata gcgggtggtc atggttccgg ctttgggtga gctccatgta ccggtcaaat
    27001 aagccagcga taataagcca accacggcct gactgacatg ctagggggaa agcatcaacc
    27061 gatagccgat gtaattaaac agcgtgacga acgaccccat cagcaaaaag ccttctgcga
    27121 acaataacgg taatccccgg tcacgccagt gcagacgaaa gttgataaat aacgtcttag
    27181 ggcgcagcga agtcgggcaa aaatggcgtg attcagggag gattttccag aacatcaacg
    27241 ccgaggccag cgcgaaacaa ccgattgccg ccagagcaat tcgccagttg aaaaagtccg
    27301 tgaagacacc actaattaag cgtccgctca tgccgccaat tgagttgccg ctgatataca
    27361 accccatcga aaaggccacg aaactggggt ggatttcctc gctaagataa gtcatgccaa
    27421 cagctgccac gccacttaac gaaagcccaa tcaaggcgcg cataatcaaa atgccgtgcc
    27481 agctggtcat cattgtcgaa agtaacgtac aaatggaggc caacagtagc gccgtgacca
    27541 tcactggttt gcgaccaatg gcatcggata gcgggccagt aaatagcaaa ccgatagcca
    27601 acatcgccgt ggaaatggac agtgaaatac tactgttcgc gggcgttaag ccaaactcct
    27661 gcgaaagcac cggaaggata ggctgcacac aatagagaag tgcaaaggtt gccagtccgg
    27721 cagagaacag cgccagggtg acgcgcataa attgcggcgt accgcgttta ataaattgat
    27781 ttggctgaga aatgctttgc ttgtcagtgt cgcttgccgg agcgccatca acagttgtag
    27841 tacggctcac ttgaaatcct tgctaaatat gcctgtggat caggcttata catagggtag
    27901 gaaaatcgaa ttgttctgtc taatatatta ataatctcaa ataagatgtt ttaaatatga
    27961 atattgaact tcgtcatctg cgttactttg ttgctgttgc ggaagagctg cattttgggc
    28021 gcgcggctgc ccgcctgaat atttcgcaac cgccgctaag tcagcagatt caggcactgg
    28081 agcaacaaat cggtgcccga ctgctggcac gaactaatcg cagtgtattg ctgacggcag
    28141 caggaaaaca gtttcttgca gatagtcggc aaatcctgtc tatggtggat gacgctgccg
    28201 ctcgcgctga aagactgcat cagggtgaag cgggggagtt gcgtattggt tttacttcgt
    28261 ccgctccttt tattcgggcg gtgtccgata cgttatcgct gtttcgccgt gattatcctg
    28321 atgtccattt acaaacccgc gaaatgaaca ctcgcgagca aatcgctccg ctcattgaag
    28381 gaacgctgga tatgggattg ctgcgtaaca cagcgttacc ggagacgctt gaacacgcag
    28441 tcatcgtcca tgaaccgctt atggcgatga tcccgtacga tcatcccctg gcaaataatc
    28501 cgagtgtaac actggctgaa ctggctgaac tggctgaact ggcgaaagaa ccctttgtct
    28561 tttttgatcc gcacgtcggg actgggctgt atgacgatat tctcggactg atgcgacgtt
    28621 accatttgac gcccgtcatc actcaggagg tgggcgaggc aatgaccatc atcggtctgg
    28681 tttccgccgg tctgggtgtt tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg
    28741 aaatgcgctg ggtgccgatt gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc
    28801 cgaaacatca tgaacaaagt ccagctgcgc gtaactttcg tattcatctg ctgaatgctc
    28861 tcaggtgagg gaaatttcag cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa
    28921 gtaaaaattt gacgacacgt attgaagtgc ttcaccatag cctacagatt atttcggagc
    28981 gcgaaaatat agggagtatg cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat
    29041 aaagcagacc aacgcgggcg cggtttatcg cctgattgat cagcttggtc cagtctcgcg
    29101 tatcgatctt tcccgtctgg cgcagctggc tcctgccagt atcactaaaa ttgtccgtga
    29161 gatgctcgaa gcacacctgg tgcgagagct ggaaatcaaa gaagcgggga accgtggccg
    29221 tccggcggtg gggctggtgg ttgaaactga agcctggcac tatctttctc tgcgcattag
    29281 tcgcggggag attttccttg ctctgcgcga tctgagcagc aaactggtgg tggaagagtc
    29341 gcaggaactg gcgttaaaag atgacttgcc attgctggat cgtatcattt cccatatcga
    29401 tcagtttttt atccgccacc agaaaaaact tgagcgtcta acttcgattg ccataaccct
    29461 gccgggaatt attgatacgg aaaatggtat tgtacatcgc atgccgttct acgaggatgt
    29521 aaaagagatg ccgctcggcg aggcgctgga gcagcatacc ggcgtaccgg tttatattca
    29581 gcatgatatc agcgcatgga cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg
    29641 cgatgtgatt caggtggtta tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca
    29701 tctgctacac gcaggcagca gtagtctcgt ggaaataggc cacacacagg tcgacccata
    29761 tggtaaacgc tgttattgcg ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag
    29821 tattcttgag ctggcacagc tgcgtctcaa tcaatccatg agctcgatgt tacatgggca
    29881 accgttaacc gtggactcat tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga
    29941 catcattatc ggggtgggcg cgcatgtagg gcgcattctt gccatcatgg tgaatttatt
    30001 taacccacaa aaaatactga ttggctcacc gttaagtaaa gcggcagata tcctcttccc
    30061 ggtcatctca gacagcatcc gtcagcaggc ccttcctgcg tatagtcagc acattagcgt
    30121 tgagagtact cagttttcta accagggcac gatggcaggc gctgcactgg taaaagacgc
    30181 gatgtataac ggttctttgt tgattcgtct gttgcagggt taacattttt taactgttct
    30241 accaaaattt gcgctatctc aatttgggcc aggaaagcat aacttagact ttcaaggtta
    30301 attattttcc tagtttatat ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt
    30361 tctttattac cggtacagac acttctgtag ggaaaacggt ggtttcccgc gcattgctac
    30421 aagcgttagc ctcccaggga aaaagggttg cgggatataa acccgtagcg aaggggagca
    30481 aagagacacc cgaagggctg cgtaataaag atgccttggt gttgcagagt gtttcaacca
    30541 tcgaactgcc ttatgaagca gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg
    30601 cgcacagttg cccaatcaat tacaccctca tttcaaacgg cctggcaaac ctgaccgata
    30661 aagtcgatca tgtcgtggta gaagggactg gcggctggcg cagtctgatg aatgatttgc
    30721 gtccactctc tgaatgggta gtgcaggaac aactgccggt gttgatggtt gtcggtattc
    30781 aggaaggttg cattaaccat gcactgctaa cagctcaggc gatcgccaac gacgggctgc
    30841 cgctcattgg ctgggtggct aaccgaatca acccaggact ggcgcattat gcggaaatca
    30901 ttgatgtgct gggtaaaaaa cttccggcac cgctcattgg tgaactgcct tatctgccgc
    30961 gcgccgaaca gcgtgaactg gggcaataca tccgcttagc tatgctgcgc agtgtgctgg
    31021 cggtagatag agtcacggtg taacgtccgc gaaattaccg acgcaattac gcaggcaatc
    31081 aataaaccgg ggagtagctg atactccccg gtcatttcac atatcatcaa cgtcgacata
    31141 atcggcgcgt gcgtggttgc cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc
    31201 gtaatttctt cgccatcagg gaaccataat cccaggctac gaccatacaa catgccaatg
    31261 gcaagaccga taaatagcgt cggtgtaaag acaccaccgg gtgcgccgga accgctactc
    31321 gccagcacgg cacacagttt acaaaggaag atcccggcaa tgatcattaa cagtggtggg
    31381 gcggttaaaa aggattgtac ggtgctatag ccgttgcccc acactgcagg tgtgaacagg
    31441 gaaagcagac ccacgatcaa cccgcctagt gccagttgcc agggcggcgc aagtttgaga
    31501 ctcacaaatc cacgatgaca ggcgttcatt aacgttaaca acagtggtcc gcacagacct
    31561 gccagcacac ctgtactgat aatcagcgca tagtcacgag cctgaaccgt cactgagagt
    31621 tgtacgttgt agagtaacgc gtcgctatga ttaatcagat tgctaaccag ccatgccacg
    31681 atggcggaaa taatcaccgg gccgagagag gccaacatca tagtgccaaa cagcacttcg
    31741 gctataaata aactgccagc aagcggggca cgataggccg cagccattcc cgccgcggcc
    31801 ccacaggcga tccataattt ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag
    31861 gcggcaaggg cagctaaaag aatcatcgca ccttcgcgac caattgcact gccgctggtt
    31921 actaccagca gcgaggcaag cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg
    31981 gtttgcaacg cttccatgta atcggtcggc gcatgagggc gttgttgggt aaatttctgc
    32041 cagcccatca gcaacaaacc cgccgccagt ccgccgagcg ccggagttag cagccgtcgc
    32101 caggaggaaa ggtttgtcgc tgcattgacc agactgccgg agtcattatt gaggaacaac
    32161 cactccagta gcagcatcgc atgacgaaac ccggcaacgg caaagacagc gagaataccg
    32221 acgactgttg cgataagcag acggcggaac atagtgcgca gatccggata gatgtgtaga
    32281 tgatgcatga gcagaaatgg gcaatgaaac acgggcgact attttgcggc aaaagaggcc
    32341 aaccgcaaaa gcagtgcgtg ctttaagaga taaaaaaacg tgacattgta acgtattatt
    32401 gccgaatcgt gcgtaatcgt caccatccgg caatattacg gtgatcctat cgaaagagtg
    32461 gtttaaccgc gacaggaatc aacagttgtg attgccattg cgccagcgtt aagcgagcca
    32521 gttcacccag tgcacggtag aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac
    32581 gtgttgacca cggaaaaagg tgccatgcca gcaattcttc gcattctgtc tggcgaccat
    32641 tctccgccaa ccacgcagcc atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt
    32701 gcttcatttc aaactgaatg cctttctcgc gcatccactg acgaagtgcc aatgttgaat
    32761 cgccaaacag cacagattcg cgatccagcc agaccgaccc ccacggcgga gacggcagtg
    32821 cccacgggcc gacaaacaaa cgctgccagg cctgggcgtg agtctcttca cactgtgtct
    32881 gaaaagccgt caccagcggc gctaatgacg cttctggtag aggccactga gtttcccagc
    32941 cgtcactggt gagtaccgca accagaggtg cggcttccgc actctctgga gcgtaataaa
    33001 acagcgcgcc caacacgcgc gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca
    33061 ttcctaacat cctgaaatgt gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca
    33121 ttcctactgt catgtgcagg ccataaaaga gcacacgccc gatcatctca ccaacaagta
    33181 tgaggattag ccccagaaca agtccggcaa cgtgaggttc acggcgacga atgagcggac
    33241 aaagccagca tccaagacct gcgcataaca ataccacacg ccagacctgt aaggaggcgt
    33301 aatctggtac cagtgcgctg gcttgttgca cggatgagtg aatagacgcc agagacaaac
    33361 cttgcagcac aatcacctcg gcacaagcga tcagcgccag aacgctaatg atggcaaatg
    33421 gtgtggtatt aaatgtaacg cgggctgccc gcagaattgc ggcagcaagt attggaccgc
    33481 tcaacaatac cgtcaggaag aaagccagcg tagtgtaacc gttatgccag gttggcacgg
    33541 tgtcgatttg atacacgcag gtcatcatcc agacgaaaat gacgccgagc gccatgctga
    33601 caagtaacca gagtttcccc aacgcttgcg gcattttacc gatgaccgcc accagccacc
    33661 atagtccgcc aacggcaaaa aatatggaac cggcagcaat ctcgttactc agaccagaag
    33721 ctccgattcg attcagcgag ttaaacgctc gcagtggcga tcccaggtgc atgacagagg
    33781 caataaaccc gatgcccatc aacagccaga ggaaaaacat gccacgaaca atgtgctgac
    33841 ggtctgcgtc atttttggcg gcgaaccagc caattccgct aacaatcagc gcgcctacta
    33901 cgcattgacc caatacggta aagataacca gtggccattc atgccatcca tttcccattt
    33961 tacacttcct ccggattagc cagataacca gtggtatcgc cggtcgggcg gctgttggcg
    34021 ttaggtttga taacgatatt gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca
    34081 gccagagtgc cgtgcttctg gcgcagctct tcaattggac cgaactcaag tgcacgcagc
    34141 gggcaggatt cgacgcatat gggttgtttc ccctcggcga cgcgcgaata acaaccatcg
    34201 cacttcgtca tgtgcccttt ttcagcattg tactgtggtg cgccgtacgg acaagccatg
    34261 tggcagtagc ggcagccgat acaaacatct tcatcaacca ccacaaagcc atcttcacgc
    34321 ttatgcattg cgccgctggg gcagactttt gtacatgcgg ggtcatcgca atggttacag
    34381 gagatggaga gataataagc gaacacgttc tggtgccaga tgccattatc ttcctgccag
    34441 tcgccgccag catattcata aatacggcgg aaactgactt ccgggccaag atctttaaaa
    34501 tctttgcacg ccagttcgca ggttttacag ccagtacagc ggctggaatc gataaaaaat
    34561 ccatattgtg tggtcatggg ctactcctta aaccttttcg atctggacaa gattgctgtg
    34621 cgacgggttt ccctttgcca gcggtgaagg gcggtgagag gtcagaatat tgatactgcc
    34681 gccgtgatcg acccggtcac caaacatatc cgctttaagc cacgcacctt gcccgatggc
    34741 ggtaacgcca ggcagaatac gcggagtcac ttttgcggca atcagcattt ctccattatt
    34801 gttaaatacc cgcacggtat cgccatgacg gataccgcgt gcctgagcat caatggggtt
    34861 gatccacacc tcttgtgggc aggcctgctg taacacatca atattgccgt agctggagtg
    34921 ggtacgcgct ttggtttggc gacttcactc agcatccagt agatgggttt gcgttcaaat
    34981 tttgctgagg ttgcgggttg ggcgaggatc acatagccca tattccctgc agattcatga
    35041 gagataaggt cttcttgctc tgttggcatc aggtcaggca acaggatatc gcaatactta
    35101 gccgaggccg tcatgaagtg gtcaatgcca acaatcatct cgcacttgct gtcatcctga
    35161 agcacctcat gggtgtgatt gatgtcgcca tgttgattga tcaatgtgtt actggcgtag
    35221 caccataaaa acttgatggg gacatccagt ttttcttttc cacgaacacc atcacgggtc
    35281 gcggtcattt tcgtaccatg gtcgatggca tctgtccatg taaaaacgga aatctgcgtt
    35341 ttaacaggat tctcgagcat cgggaaccat tctaccccca gatcccagct accttcgcgt
    35401 acacctgagt tgccgccgtt tatgccgacg ttaccggtaa gaacggaaag catggcaata
    35461 gcgcgggacg tttgttcgcc gttggagtgt cgttgtggcc cccaaccctg acaaatataa
    35521 gcaggttttg ctgaaccgat ctctcgtgcc aactggataa ttttttctgc cgggatgctg
    35581 gtgatttttg ctgcccattc cggcgtttta gctatgccgt caggcccttc gcccagaata
    35641 taggctttat aatgcgcgtt acgtggtgcg ttggcgggca gcgttttttc atcgtaacca
    35701 acacaatatt tgtcgagaaa tggctgatcg accatgtttt cagtaatcag tacccaggca
    35761 atcgcacagg ccagtgcgcc atcggtgcca gggcgaatgg gcagccattc atcttcacgc
    35821 ccggcagctg tgtcgttata gcgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg
    35881 cgggcttgct cgacgtagta agtgacacca ccgccgctca tccgcgtttc tgccgggtta
    35941 tttccgaaca taacgaccag tttcgtattg gcgatatcat ccgggctgtt gccatcattg
    36001 gcaccgaaca tataactcat tgcggcactg atctgtgcgg tactgtagct gccatagcga
    36061 ctgagaaaac ccccgcaaga gttcatcaga cggtacggga cgttcgagtt ggtgatattt
    36121 ccgccatcta cgcctgttcc gtacaggaca tgtacagcct cattgccgta atctttcagg
    36181 atccgccgaa gattatcact gatggtatcc agggcttcgt cccaacttat ccgttcaaat
    36241 ttaccttcac cgcgctcgcc gacgcgcttc atgggatatt tcaacctgtc aggatgattc
    36301 atccgtcggc ggatagagcg cccgcgtaaa catgcccgaa cctgatgatt accgtaaacg
    36361 tcgtcacctg tcgtatcaga ctccacccag tacacggtgt catctttcac atgcaaacgt
    36421 agcagacagc ggctcccgca gttaacggtg caggaactcc agaccgtttt ctcttctacc
    36481 ggagcctctg ccgcccggac catttgggaa aatggcagag tgaaagcact gcttgccagc
    36541 gcgagactgc caagtgcgga ggttttcatc agacttctat ggctgatttc agccttcatg
    36601 agcgcctctg tggtatggat tttcatcatt actcacttat tgcttttcaa acaaaatgtc
    36661 atgccagaat ttatggttgt agtgggttat attttttcga tctcgaccag attagtgtgc
    36721 tgcgggtttc ccttcgccag tggtgaaggg cgcagagtgg ttagcgtatt cacacatccg
    36781 ccatggtcga ttttatcgcc agacatattg ggctcgtgcc aggctccctg gcccatagcg
    36841 ctaactccag ggagaatgcg tggtgttact ttggctggta gccgaacttc gccacgatgg
    36901 ttaaacaccc gcaccatatc gccgttggca atcccacgtt tctgtgcatc tatagggttg
    36961 atccacacct cctgacggca ggctgccttc aggacatcaa tgttgccgta ggtcgagtga
    37021 gtacgggatt tgtaatggaa accaaacagt tgcagtggga aggttctacg ttcaggggag
    37081 tcccagcctt caaaggttga ggcataaact ggcagtgggc ttatcacttc atctttttcc
    37141 agttcccagg tacgggcaat ttccgccagc ttgctggaat aaatttcaat cttaccggaa
    37201 ggcgttttaa gtggatttgc ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatgg
    37261 ccattgggat ctttacgctt atagataccc atttttttca gttcgtcgta agacggtaac
    37321 gccggatctt tggcaagcat tttggcgtac agatgttgta accattgttc ctgcgtgcga
    37381 ccttctgtga acttttgata gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc
    37441 cagtaaatcg gtttacgttc gaatttttcg ctggtgactg gctggaggaa aatgagatat
    37501 cccatgttac cggcgtagtc gttaggaata atatcttcct gctcaacggt catcaggtct
    37561 tccagcagaa tgtcggcata ttttgccgat gaggtcataa agttttcgat gaccacaatc
    37621 atttcgcatt tcgattcgtc ctgcagaatt tcatgcgttt tgttgatgtc agaatgctga
    37681 ttaacgaggg tatttcccgc gtagttccag atggactcca ttgccagacg gatggatgga
    37741 cttgaagtag ttgactcaat gctacttaag gtcctgatat gactgacatt aatcatgata
    37801 acactgtata tccatccagt tctttctctg ggtctcccta tcggtatcag caagtctaca
    37861 cctcaagccc tgacacaagg ctgacacaaa tcaatctact cagcgtagct aagcctcagt
    37921 tagtccgcag agccaatggt cgatatacta tccgcttcag actcaaggga cagaccacac
    37981 cgtttctttc agtatcgacc cgcagcacag acaggagagt agccacaatg cgtcagagag
    38041 agcttgcagc caccgctaag gccttcatgt tggataggcc tgaagtctca ctacaggagc
    38101 ttacagagca tctccggtct atggctgagc agttcctgac agacgctagt gacgattact
    38161 ggaatggact tgaggtcgct actctggtcg acgagaagtc caacctgaag gagctagccg
    38221 ctacgcaagc cttgagtctg gaccagcaga agggcataag gttagcgctg gaagtcctga
    38281 ccgcagcaca gcagagagtc gacactggtg atacctctgg tctgattaag ctgattgatg
    38341 ataataacct tactgatgac tcaactatag gcgactctac gtcaattctg aataatgagc
    38401 agggggatag accggcagtt ttcacacagg agcgccagtc atctgtagtt ttttcaagcc
    38461 tagtgtcctc gctgctggct gagaaggtcc agaccctgaa gacatccagc tacaaggacc
    38521 tctcgtcctc actcaatact gtctcccggt tcctgcctga agacatggac ctgatgtccc
    38581 gctctgggtg gctggctgtc agggactcta tgctggcttc tgaggtgaga ccatcgacaa
    38641 tcaacaagct gctgaccaaa gcgaagatgt gtctcgatta tggcctgatg aatgggcaac
    38701 ttgagggccg caaccctatt gaacggatga agctgaccaa ggatatcgac tccaagcgca
    38761 gagcgtt
//
LOCUS       DAJTYH010000027        38201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTYH010000027 DAJTYH010000000
VERSION     DAJTYH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..237)
                     /gene="yefM"
                     /locus_tag="OO609_001380"
     CDS             complement(<1..237)
                     /gene="yefM"
                     /locus_tag="OO609_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6632857.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEK"
     gene            520..561
                     /gene="hisL"
                     /locus_tag="OO609_001381"
     CDS             520..561
                     /gene="hisL"
                     /locus_tag="OO609_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6632858.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    537..660
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            707..1606
                     /gene="hisG"
                     /locus_tag="OO609_001382"
     CDS             707..1606
                     /gene="hisG"
                     /locus_tag="OO609_001382"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6632859.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1612..2916
                     /gene="hisD"
                     /locus_tag="OO609_001383"
     CDS             1612..2916
                     /gene="hisD"
                     /locus_tag="OO609_001383"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6632860.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2913..3983
                     /gene="hisC"
                     /locus_tag="OO609_001384"
     CDS             2913..3983
                     /gene="hisC"
                     /locus_tag="OO609_001384"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6632861.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3983..5050
                     /gene="hisB"
                     /locus_tag="OO609_001385"
     CDS             3983..5050
                     /gene="hisB"
                     /locus_tag="OO609_001385"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6632862.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5050..5640
                     /gene="hisH"
                     /locus_tag="OO609_001386"
     CDS             5050..5640
                     /gene="hisH"
                     /locus_tag="OO609_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6632863.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5640..6377
                     /gene="hisA"
                     /locus_tag="OO609_001387"
     CDS             5640..6377
                     /gene="hisA"
                     /locus_tag="OO609_001387"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6632864.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6359..7135
                     /gene="hisF"
                     /locus_tag="OO609_001388"
     CDS             6359..7135
                     /gene="hisF"
                     /locus_tag="OO609_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6632865.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7129..7740
                     /locus_tag="OO609_001389"
     CDS             7129..7740
                     /locus_tag="OO609_001389"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6632866.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7926..8903)
                     /gene="wzzB"
                     /locus_tag="OO609_001390"
     CDS             complement(7926..8903)
                     /gene="wzzB"
                     /locus_tag="OO609_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6632867.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9049..10169)
                     /gene="ugd"
                     /locus_tag="OO609_001391"
                     /pseudo
     CDS             complement(9049..10169)
                     /gene="ugd"
                     /locus_tag="OO609_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10419..11825)
                     /gene="gndA"
                     /locus_tag="OO609_001392"
     CDS             complement(10419..11825)
                     /gene="gndA"
                     /locus_tag="OO609_001392"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6632868.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11934..12293)
                     /locus_tag="OO609_001393"
     CDS             complement(11934..12293)
                     /locus_tag="OO609_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632869.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12290..12796)
                     /locus_tag="OO609_001394"
     CDS             complement(12290..12796)
                     /locus_tag="OO609_001394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632870.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12942..13505)
                     /locus_tag="OO609_001395"
     CDS             complement(12942..13505)
                     /locus_tag="OO609_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632871.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13712..14650)
                     /locus_tag="OO609_001396"
                     /pseudo
     CDS             complement(13712..14650)
                     /locus_tag="OO609_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14713..15087)
                     /locus_tag="OO609_001397"
     CDS             complement(14713..15087)
                     /locus_tag="OO609_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6632872.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15091..16239)
                     /gene="wzy"
                     /locus_tag="OO609_001398"
     CDS             complement(15091..16239)
                     /gene="wzy"
                     /locus_tag="OO609_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6632873.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16259..17164)
                     /gene="rfbG"
                     /locus_tag="OO609_001399"
     CDS             complement(16259..17164)
                     /gene="rfbG"
                     /locus_tag="OO609_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6632874.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17157..18047)
                     /locus_tag="OO609_001400"
     CDS             complement(17157..18047)
                     /locus_tag="OO609_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6632875.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18077..19333)
                     /gene="wzx"
                     /locus_tag="OO609_001401"
     CDS             complement(18077..19333)
                     /gene="wzx"
                     /locus_tag="OO609_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6632876.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19349..19894)
                     /gene="rfbC"
                     /locus_tag="OO609_001402"
     CDS             complement(19349..19894)
                     /gene="rfbC"
                     /locus_tag="OO609_001402"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6632877.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19899..20777)
                     /gene="rfbA"
                     /locus_tag="OO609_001403"
     CDS             complement(19899..20777)
                     /gene="rfbA"
                     /locus_tag="OO609_001403"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6632878.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20836..21735)
                     /gene="rfbD"
                     /locus_tag="OO609_001404"
     CDS             complement(20836..21735)
                     /gene="rfbD"
                     /locus_tag="OO609_001404"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6632879.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21735..22820)
                     /gene="rfbB"
                     /locus_tag="OO609_001405"
     CDS             complement(21735..22820)
                     /gene="rfbB"
                     /locus_tag="OO609_001405"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6632880.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23193..24086)
                     /gene="galF"
                     /locus_tag="OO609_001406"
     CDS             complement(23193..24086)
                     /gene="galF"
                     /locus_tag="OO609_001406"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6632881.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24261..25655)
                     /gene="wcaM"
                     /locus_tag="OO609_001407"
     CDS             complement(24261..25655)
                     /gene="wcaM"
                     /locus_tag="OO609_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6632882.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25666..26886)
                     /gene="wcaL"
                     /locus_tag="OO609_001408"
     CDS             complement(25666..26886)
                     /gene="wcaL"
                     /locus_tag="OO609_001408"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6632883.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26883..28163)
                     /gene="wcaK"
                     /locus_tag="OO609_001409"
     CDS             complement(26883..28163)
                     /gene="wcaK"
                     /locus_tag="OO609_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6632884.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28343..29821)
                     /gene="wzxC"
                     /locus_tag="OO609_001410"
     CDS             complement(28343..29821)
                     /gene="wzxC"
                     /locus_tag="OO609_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6632885.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29823..31217)
                     /gene="wcaJ"
                     /locus_tag="OO609_001411"
     CDS             complement(29823..31217)
                     /gene="wcaJ"
                     /locus_tag="OO609_001411"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6632886.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31272..32642)
                     /gene="cpsG"
                     /locus_tag="OO609_001412"
                     /gene_synonym="manB"
     CDS             complement(31272..32642)
                     /gene="cpsG"
                     /locus_tag="OO609_001412"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6632887.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32835..34271)
                     /gene="cpsB"
                     /locus_tag="OO609_001413"
     CDS             complement(32835..34271)
                     /gene="cpsB"
                     /locus_tag="OO609_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6632888.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34274..35497)
                     /gene="wcaI"
                     /locus_tag="OO609_001414"
     CDS             complement(34274..35497)
                     /gene="wcaI"
                     /locus_tag="OO609_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6632889.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35494..35973)
                     /gene="gmm"
                     /locus_tag="OO609_001415"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35494..35973)
                     /gene="gmm"
                     /locus_tag="OO609_001415"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6632890.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35973..36941)
                     /gene="fcl"
                     /locus_tag="OO609_001416"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35973..36941)
                     /gene="fcl"
                     /locus_tag="OO609_001416"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6632891.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36944..38065)
                     /gene="gmd"
                     /locus_tag="OO609_001417"
     CDS             complement(36944..38065)
                     /gene="gmd"
                     /locus_tag="OO609_001417"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6632892.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38091..>38201)
                     /locus_tag="OO609_001418"
     CDS             complement(38091..>38201)
                     /locus_tag="OO609_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6632893.1"
                     /translation="DGTVVGARSSVFKSLPANVVCRGNPAVVIRERVETE"
BASE COUNT        10202 a         9576 c         8604 g         9819 t
ORIGIN      
        1 cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc tccgggcgtt
       61 agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt cgagtgacat
      121 cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat cttcaacggc
      181 tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg tacgcatgta
      241 aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact atttattaat
      301 tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct ctttttggtg
      361 ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt cattaaacaa
      421 atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag tggtttaggt
      481 taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt tcaatttaaa
      541 caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat tcagatcttc
      601 cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat
      661 tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga cagacaacac
      721 tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac gcgaattact
      781 ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa
      841 catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg taatggacgg
      901 cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg
      961 cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg gcggctgccg
     1021 cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct taaacggtaa
     1081 acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga aaggtatctc
     1141 ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg ggctggcgga
     1201 tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc tgcgcgaagt
     1261 cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa tggaagaatc
     1321 taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc aggcgcgcga
     1381 atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca tcgccctgct
     1441 gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc gcgtagcgat
     1501 gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga aagcactggg
     1561 tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc catgagcttt
     1621 aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct gttaatgcgc
     1681 ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct cgataacgtg
     1741 aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa aaccacggtt
     1801 accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct gagcgacgag
     1861 ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac tgcgcaaaaa
     1921 ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt cacgcgcccg
     1981 gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc aacggtatta
     2041 atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg ctcaccgccg
     2101 ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga cgtgtttaac
     2161 gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt gccgaaagtg
     2221 gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca ggtaagccag
     2281 cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct ggtgattgct
     2341 gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc tgaacacggc
     2401 cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg cgttgccgag
     2461 gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca ggcactgaac
     2521 gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc caaccagtac
     2581 ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga tggcatcacc
     2641 agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga ctacgcctcc
     2701 ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag cctcgggctg
     2761 gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt ctccgcgctg
     2821 gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa aaatgccgtt
     2881 actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt actgatttag
     2941 cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg ggcggtaacg
     3001 gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag cttactcagc
     3061 aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat tacgcgcaat
     3121 atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac
     3181 tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc ccgccaacgt
     3241 acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca gtgccgacgc
     3301 tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc gtaaaagtgg
     3361 tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag gattttcgca
     3421 ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct tatatcgagt
     3481 tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg gcgattttgc
     3541 gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg ctggcaaacg
     3601 aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg acgccggttg
     3661 ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc gaacgggtgg
     3721 cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc ccctgcgtgg
     3781 agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc tccagtgcag
     3841 tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa caaccctctt
     3901 taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc gtcattgacg
     3961 ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc gcgatggaac
     4021 cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac tcgcctttga
     4081 accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc tggtgatgat
     4141 cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg atggcccgca
     4201 caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag tgctgatttg
     4261 tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac tggtggaggg
     4321 ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg atcgcgcgac
     4381 cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc gcgaaaccct
     4441 gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc atgtagtgcg
     4501 caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag gtggcagcaa
     4561 gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca cccacggcgg
     4621 tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc acaccgtcga
     4681 agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca aacgcggtat
     4741 ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg cactggatat
     4801 ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg tgggcgatct
     4861 cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg gcgtgacact
     4921 acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt tcaaagcctt
     4981 tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct cgtcgaaagg
     5041 agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa ctctgtgaag
     5101 tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga cattgtgttg
     5161 ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat ggatcaggtg
     5221 cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct gggcatctgc
     5281 ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga cttgctgggc
     5341 atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc acatatgggc
     5401 tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga agacggcgcg
     5461 tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat cgcccagtgt
     5521 aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg cgtgcagttc
     5581 cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct ggagatgtga
     5641 tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat cagggcgatt
     5701 acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat tacgctgcgc
     5761 aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg gctaagcgtc
     5821 aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag gttggcggcg
     5881 gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg cgcgtggtgg
     5941 tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa cgtttcggtg
     6001 ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac aagcaagtgg
     6061 cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg gaaacctatc
     6121 tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc acgctggcgg
     6181 gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg gcgtttcagt
     6241 cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact ggcgtgcgcg
     6301 gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag gccatcgcat
     6361 gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt
     6421 acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac gctacgccga
     6481 agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg gccgtgtggt
     6541 agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt gtgtggcggg
     6601 tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg ataaaatttc
     6661 catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg atcgctttgg
     6721 cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg gtaaatatca
     6781 tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg aaacgctcga
     6841 ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca tgatgaatca
     6901 ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg aagtttgcca
     6961 cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg aagccttccg
     7021 cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa taatcaatat
     7081 tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat gttaacagaa
     7141 caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt gattgtgcaa
     7201 cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc cttagacaaa
     7261 accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact gtggatcaaa
     7321 ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg cgacaacgac
     7381 acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac cagtagctgc
     7441 ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact gctcgccgaa
     7501 cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag cggcactaaa
     7561 cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc aacggtacat
     7621 gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct ggtgctgttg
     7681 caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg gcatcagtga
     7741 gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc cgatgcgcaa
     7801 acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc gccagcatcg
     7861 catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt aaatgcacaa
     7921 taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat ccccgcgcca
     7981 accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg actatcgcga
     8041 cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag atcatcaaac
     8101 tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg tgagaacacc
     8161 aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc tagaaggaac
     8221 aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac ctgcgcctga
     8281 ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgcg
     8341 accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc aatgttgtcc
     8401 ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat gtattgggcc
     8461 aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa tggtaattgc
     8521 tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt atccagcgtt
     8581 tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg caaatccgat
     8641 actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc agcaatttgc
     8701 cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc tactgccaaa
     8761 taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat cttgccacgc
     8821 cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa
     8881 acattattat tttctactct catcattacc ctaactgacg gaaatttcca agtccgcata
     8941 gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg aattattgcg
     9001 gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt agtcgctgcc
     9061 aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg ccatacggtt
     9121 ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca ggcgagagtt
     9181 gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc ctttcgcctt
     9241 gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc cgctcttcat
     9301 aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga gatcaggtta
     9361 ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag acaataacca
     9421 ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag acaaacgcct
     9481 tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag ttcattaaag
     9541 tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc tgcttcagtg
     9601 gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa caacgcagca
     9661 aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg gagattatcg
     9721 taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc agtacgatat
     9781 ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac cataaccgca
     9841 taaggattta tctcaactac gtctttaatt actgattcta cactggatgt attgaaataa
     9901 ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc atcccggtag
     9961 gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa aaactgctga
    10021 atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg tgacggtaaa
    10081 atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga caagcctaca
    10141 tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact taactgtgaa
    10201 tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg ttgtcagatg
    10261 gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg tttattaagt
    10321 taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc ccggtgcaat
    10381 atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca ttcagtatgg
    10441 aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc acgctgtgcc
    10501 tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc aaccgcagcg
    10561 gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc gcgcagcgcc
    10621 tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt agcgatctgc
    10681 ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat gatgcagcca
    10741 gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc ttcagacgca
    10801 gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata cagcgcactg
    10861 cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg accagagaga
    10921 actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc aaacacagac
    10981 tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt ccatttaccg
    11041 gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc gtcttcatct
    11101 tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc accgttattc
    11161 cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc tttgagcaga
    11221 gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg aaccatcttc
    11281 acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc gtcttctgcc
    11341 acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt ctgcccgcca
    11401 ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc ggtaccgatg
    11461 aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg gaagaaggta
    11521 ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga atcaatcgca
    11581 gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt ttccagagat
    11641 tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt ttcagcaatc
    11701 acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc acgactttcg
    11761 atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat ctgttgcttt
    11821 gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta tattattaac
    11881 cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc ttcttacttt
    11941 atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg atacttttct
    12001 ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt aaaagatttc
    12061 atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact aatagtccaa
    12121 tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata gaagtaaata
    12181 ccatcctcca agcctaattg gataagatta tctccatata actcactata ttcacttgct
    12241 ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt caagtattca
    12301 gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata atacacacaa
    12361 ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt aaacttgcaa
    12421 ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac ctgtcacctt
    12481 gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat acccaaagta
    12541 ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca tcactgccaa
    12601 ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt cgagcttgtt
    12661 ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat atatttcctc
    12721 cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa taaatgccac
    12781 caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt ttcgctatca
    12841 atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca caagcaattc
    12901 caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt tctgagcgaa
    12961 tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat atatgcataa
    13021 ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat aatgcctgga
    13081 taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat tacaaaaaaa
    13141 cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc taaagccatt
    13201 aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac aacaattgca
    13261 tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc ccaatcaccg
    13321 ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa taaaataaca
    13381 aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg acgtttaata
    13441 atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt aaattgcccg
    13501 accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca taggcaataa
    13561 aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat gacaatacaa
    13621 tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca tacaaataca
    13681 acggcaattc aatcatagta aaatactcta tttacattat cagtttatag tttttttcag
    13741 aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc aaaataatgc
    13801 cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag gcagtcggaa
    13861 atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct aaaattatag
    13921 acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt tcaagcgagt
    13981 aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat agtttgctca
    14041 cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc atacgtaatt
    14101 ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga catggaaaag
    14161 atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta aaattccctt
    14221 taaagatagt ctgaacagta ccagtcaaca atttatttcc tcagcgatgc ccttttctgt
    14281 atgtattttg atctccattc tcttgacgac atcctacaac catatccaat tgatttttta
    14341 tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct ccatcaacca
    14401 ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc cccttgcctt
    14461 tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct gtactgtcag
    14521 tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg aaatcattta
    14581 tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg atattaaaag
    14641 agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat tgtgttttta
    14701 acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta aaaccaaact
    14761 aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagat caaataaaac
    14821 gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc tagccttggt
    14881 tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg ctgcacatat
    14941 aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac acgcataaaa
    15001 taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa aaaaagaact
    15061 ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga ggttattact
    15121 aatttggata ttttctatag aaaataccca taaaactaca acgcctataa taaaaattcc
    15181 catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa aaagtattaa
    15241 cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa agataagcaa
    15301 cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta tctcaagagg
    15361 tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc caaacaagaa
    15421 cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa cagagtcaga
    15481 atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc tgcccaatat
    15541 tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata acagtgataa
    15601 tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca ctattatgag
    15661 acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa aaaagtagaa
    15721 ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca atgataagtg
    15781 ttcataaatt aaattaaaat ttaacaaacc gaaattcaac aaaattatgt atgaaatata
    15841 taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct cacatgaaaa
    15901 tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa aaaggtatgg
    15961 ttgcacatca aacagtatat catttatttt attaccagct aataagccga caaagaagcc
    16021 ataaaaagga aataaaagca accatataaa aagactgaca catttttggg tgattgaaaa
    16081 attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata ataaatatcg
    16141 caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa tcagttcaat
    16201 acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt gatgtttttt
    16261 aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt ttccgcctta
    16321 acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc aattgattat
    16381 tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt cgataccact
    16441 tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt gctcccactt
    16501 ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac cacgaataat
    16561 ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct tgtgcagcat
    16621 ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt ataacatatg
    16681 tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac tgagccttat
    16741 caactaaatt ttttcgccct gatggataat atgcaattac attgctggaa taacaattcg
    16801 cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac caaatatcgt
    16861 cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta attaatctat
    16921 aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt ataattgagg
    16981 gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat aaatcatctg
    17041 atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat tgttcctcaa
    17101 tccagcattc gccattatac gtagccataa gtacagctac tcttttttga tcagaactat
    17161 gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc caaaatatgt
    17221 tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt atgaattaaa
    17281 gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct aagcaaaaga
    17341 aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat gttaatccca
    17401 cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg aatgatatga
    17461 actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata atcaataaaa
    17521 agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat ggttccggat
    17581 gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat tttctctcga
    17641 agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct atcaataaaa
    17701 actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt tttgataaac
    17761 tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc tttttcaaga
    17821 gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctacc aagacaaatt
    17881 aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt cctattatcg
    17941 actactacaa cgtaacaatt ttgctccttt agttcagtga tcagtgcatt cagattttta
    18001 agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat ccaagtgact
    18061 cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa agccattgta
    18121 ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc agtaacccat
    18181 atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca caatgaatca
    18241 aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc attgccagcg
    18301 cattgaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta atttgataat
    18361 tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg attatcaccg
    18421 gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta aattctttaa
    18481 agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca ggtattattc
    18541 ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct gcccccataa
    18601 tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata ttacttactg
    18661 tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa tgaacccccg
    18721 aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata actcttgcaa
    18781 atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat atggccggaa
    18841 ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca aatttttcat
    18901 ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg caaatagcca
    18961 acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt agataattaa
    19021 caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat agcaccaaaa
    19081 aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga tgaattgcaa
    19141 tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac ccaacaagtg
    19201 caattgcaat tgtataaaca ccgaaacctt caggccccaa acctcgagcg agaaatccta
    19261 gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag acactatttt
    19321 ttattatgct cattattaca tttctttatc atataaaata atcagcatct acaaattttt
    19381 gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct atatttatac
    19441 ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta tttgttttgt
    19501 agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct tccggtatcc
    19561 ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca aatgttggtg
    19621 atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag cgtaccagtt
    19681 ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc ttagttgatt
    19741 tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg aaaactttct
    19801 gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt tcaaaaatta
    19861 gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt aatcattttc
    19921 agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt cacttgctca
    19981 gcatcaataa agcctttacg gtgagcaatt tcttccgggc aggaaacttt cagcccctga
    20041 cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg tgtccccgtg
    20101 tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg gtccatataa
    20161 atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag gtttttcgcc
    20221 atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt actttttggt
    20281 tcctgcggtt tttcttccag actaattgcc gtaccgttat catcaaattc caccacaccg
    20341 tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc tctgttaaca
    20401 gcggtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc aagtaccagc
    20461 gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc cagaccatcc
    20521 ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc accaagcaat
    20581 tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg aatacccgcc
    20641 aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg tagcagttgt
    20701 ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc cagaataata
    20761 cctttacgcg ttttcatttc accattcctt ttaattcatc tcgctctagc atcatgagcg
    20821 agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca tacgtttcac
    20881 gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt cggtattgag
    20941 gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca cggcattaag
    21001 tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca gagcggcata
    21061 atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa cttctggttt
    21121 atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag cccccgttgg
    21181 tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca ggcgcaacat
    21241 cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga aaataagatg
    21301 ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac cgtagacatt
    21361 tagcggtgct gttgcatccg tttccagcca tggcgtgtca ccgttgcctg ggaaaacata
    21421 atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg caatcgcttc
    21481 gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt tgtctactgc
    21541 agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg tttcagccac
    21601 accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga gcgcaatcaa
    21661 gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg ttttgccgaa
    21721 aagcaggata ttcattactg acggccctca tagttttgtt caatccacga ctgataagta
    21781 ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac cgttttacga
    21841 atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat tttatcggca
    21901 tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat ttgctcacga
    21961 taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag cactacgtcg
    22021 atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt accttcggtt
    22081 acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg aatttgatcc
    22141 cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac caatggaata
    22201 agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac aatggttggt
    22261 aaaccataag tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt agacgcagaa
    22321 tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc ttcgttatta
    22381 tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat atgatgaaag
    22441 cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc cgcttctaaa
    22501 agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt tattgaacgg
    22561 tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg tgcgaaaata
    22621 cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg ttcagaatca
    22681 gaaacatcag cgagtgattc caggttcccg gcgtatgtta atttatcgac attaacaaca
    22741 ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa ccctgcgcca
    22801 ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc tgtgaaatag
    22861 actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg gcttaacagc
    22921 taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg cgccaaatcc
    22981 gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa aatcctggaa
    23041 aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata aaaacggcaa
    23101 cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg ttataatttt
    23161 cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt ctcaatacct
    23221 ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa cgcctgcata
    23281 tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc atcaacggat
    23341 tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca tgcaccaggc
    23401 tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc tacggccatg
    23461 atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac aatgcggctg
    23521 actttaccct cacggtccag cggctcttta gtctggatga cggagtattc agagaggtca
    23581 cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg tgcaatcatg
    23641 gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac gtctggcagt
    23701 accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga gtggcctaaa
    23761 cctaaaggtt cgccctgacg cacgttcata atgatcacgc ccggcggaca gatggactgt
    23821 acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa ctcataagag
    23881 gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag gatttctttg
    23941 atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc gacgattggt
    24001 agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag acccgctaca
    24061 ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt tgctgaatta
    24121 tagcttgttc gatttttttc gccagcatca attaccctga attgattact gaattacttg
    24181 tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat ggctccattt
    24241 ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt aatcttttcc
    24301 acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc tctttcattc
    24361 accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa gacgccgcga
    24421 acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga ttcctgcatc
    24481 acattgatat ttcgcataaa aagatgttgc ggcttgttgt gtaactccag cgaggcacgc
    24541 ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga gatttggatg
    24601 ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac tcggaaattt
    24661 tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat cccggcgcta
    24721 ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc gactgtcgcg
    24781 ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt gatattacga
    24841 ataacaaaat gtttaccatt ttcaacatgg atcaactgcc gacaatccga tcccgtgata
    24901 ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc ataagtgctt
    24961 cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt gatacgctcg
    25021 atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca ttcaatggcg
    25081 tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat ctggttatga
    25141 aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc gatggtcagg
    25201 ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg gccaaagccg
    25261 ctcatcgcca gccctttgat ggtgcagtca gaaccacgca catccagggt aatgttatgc
    25321 ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa tcgccctctg
    25381 ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat gaagatgccg
    25441 gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc ttcgctgaag
    25501 gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt aacgctttgt
    25561 cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga gcttgccgtc
    25621 aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata aagcctgcag
    25681 caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga ctttttcgca
    25741 cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg ccgccagacg
    25801 ttgtgccagt gcgcgagcat cgttctcagg cagcagccag ccggatttat cggcctccac
    25861 cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg ccatcgcttc
    25921 cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg atggcaacag
    25981 gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa agcccggcat
    26041 ctctaccaca tcttccagtt gatattgttc gatgatggtg cgcaggcgtc gttcccacag
    26101 gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact gacggcaggc
    26161 ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata tgatttccag
    26221 cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca cgcccatacg
    26281 cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg cccacagatc
    26341 gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg gagtgtagtg
    26401 gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa ttttgccgcg
    26461 aatgataccc agttcgcgta gttttgctgc ggttaccccc gccgggccaa aatgagcgat
    26521 gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca aaatcaggtt
    26581 ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt tacgatgaat
    26641 gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcccg caggttcgtc
    26701 ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt gggtgttctc
    26761 tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa taaacgcggt
    26821 aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga agaagccgac
    26881 cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat tcccgtctga
    26941 cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg gcctaaggta
    27001 tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat cgccatctct
    27061 ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat ggcaatcgcc
    27121 ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac ggtgagttca
    27181 caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac tacgtggtaa
    27241 cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc gtctttgtta
    27301 tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc atcgagaatg
    27361 cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc gagacgtttg
    27421 tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc ggcaacgtcc
    27481 agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc gaccagccac
    27541 gcggtatcga cgccatgttc cacttttgcg gtggtgatat ttctgcgttt catcaaatca
    27601 aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata gttcgccagt
    27661 tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa cagcggcttt
    27721 ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag atcgacaaaa
    27781 aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac gaagtcggtg
    27841 aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg tgagagcaat
    27901 acctgatgct ggtagcggcg acggaggact tttttaatgc gcccgacaac gcccgccgcg
    27961 ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg gttgagcagc
    28021 caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg attgaggatg
    28081 ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc gcaagtgtgg
    28141 ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt gaggctgtgt
    28201 ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta tcaggcctac
    28261 aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc cggcaaccgt
    28321 tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt ttttcgctgc
    28381 gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca atcatcacca
    28441 caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc gccagttgcc
    28501 ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag agataaaacg
    28561 gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt ttaatcatca
    28621 cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc gtgacgccga
    28681 ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat gttttgaata
    28741 cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa ccaatcgggt
    28801 tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga ataatgctgt
    28861 tccacttttc gccgaatacc agcggtacga agttattcga caccaccatc agccccagca
    28921 gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta acacgcagct
    28981 tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg atgattgggt
    29041 tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc cctgccacgc
    29101 ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga tagttgatga
    29161 tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac gccagcgaga
    29221 agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt gttctcaccg
    29281 cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa tgggcgctaa
    29341 ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg attttgttga
    29401 actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc accacaaacg
    29461 ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg tcgccgatga
    29521 catcactcaa caaaaacacc gccacacata ccacaagccc cagcccgacg ttcagccagt
    29581 acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag ttagcgatac
    29641 cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc agcaggccga
    29701 actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg aggccgatga
    29761 tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca cgtaagctca
    29821 tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac gattttgata
    29881 tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg tttttccatt
    29941 ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc ggtaatgccc
    30001 ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg ttcgttatgc
    30061 gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac attgataaac
    30121 tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt gacgcgcgga
    30181 tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat ggaacggaac
    30241 ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa aataaccggc
    30301 cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga gatcagcagc
    30361 agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt aacccccgaa
    30421 agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag acgtgaatgg
    30481 agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt atccgccagt
    30541 tgatggacca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat atagacgtta
    30601 tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc ccagtcgtta
    30661 gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc taaccacggc
    30721 tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccgc tacggcgacc
    30781 atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat acatgaacgg
    30841 caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt cagttgcgtg
    30901 tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa ggtccagttt
    30961 tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg ataaaaatcg
    31021 gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa caggtgcatg
    31081 tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa catgatggtg
    31141 atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc tcgctcgcgc
    31201 ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt ggggtaaggg
    31261 aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc ttccatcagc
    31321 ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt ggaggtgcgc
    31381 aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc caccgccagc
    31441 gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg cgccagtttg
    31501 ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag ttcgcccagc
    31561 gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat gccgctgtcg
    31621 cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta gatggcgtct
    31681 tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac cggcgtgccg
    31741 cctgcagcag tcaccacatc aatagtgttc caagagagac gcggatcatg gatgatcttc
    31801 gcgccgggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta gtagccctcg
    31861 ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc aaaggcaatg
    31921 cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca ttccggcagc
    31981 agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac tttgattaat
    32041 tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac cggacccgct
    32101 gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac attgatataa
    32161 ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg accgcgtttg
    32221 gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc gcgcagtccg
    32281 gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt gtaatccatc
    32341 ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa cgtggcgaaa
    32401 tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc atcctgtaaa
    32461 ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc gcctaacaca
    32521 atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc gatatcttca
    32581 ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt taattttttc
    32641 atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccaa ttttattttt
    32701 gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc gcgtcttatc
    32761 aggcctacaa aaccgaaccg taggtcggat aaggcgttca cgccgcatcc gacaaacagc
    32821 gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac atcatcctct
    32881 tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc cgggttttcc
    32941 aggcagtgcg tcgcccccag cggaatataa atggactcgt tttcaccaag cagtttgata
    33001 tcaccatcaa tggtgacttt tgccgttccc gcgaccacta cccagtgttc cgcgcggtga
    33061 tggtgcatct gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt cacctggtag
    33121 cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc gcgatgcacc
    33181 cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac atcctgtacc
    33241 gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag atctttcacc
    33301 ccgacggtgg tgaccaggcc tgattcggca tacacatagc tgttttcagt tttgtgatta
    33361 atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc ccataatgaa
    33421 gaccaggaac caacatcgct ccagcccgca tccatcggca ccacaacagc atctgccgta
    33481 ggttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc ttcttcatcc
    33541 acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcgca ggcatcgagg
    33601 atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa caggaacatg
    33661 ccgctgttcc agtaatattc gccgcttgct acataggcct gggcggtttc cagattcggt
    33721 ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccac agacacttca
    33781 ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc aaaggtcacc
    33841 agcttgcccg cttcggcata aggcatggca ttacgcacgg cggcacggaa cgcgtcttca
    33901 tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc cgggctatga
    33961 cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc tgccggttcg
    34021 agaataatgt tctcggtgag tttgttcagt tgacgcagct gttccgcgac aataaagcgg
    34081 tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag gcggcagatg
    34141 gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg gtaaagtacg
    34201 cgggaaagcg gccataaacg gctaccggag ccacctgcca tcacaactgg atagagtttc
    34261 gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg ttctctttat
    34321 cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc aggagcgcct
    34381 gacgaatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc gcaatacccg
    34441 gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc accgcgttac
    34501 cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt
    34561 tttgcaccac cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg tcatacgatt
    34621 gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt tccagccgcg
    34681 ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc aggagatcgg
    34741 cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag taaagaatga
    34801 tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg gcgtctgcaa
    34861 catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgtta tccgccgcca
    34921 cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg ttatcgacgt
    34981 tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgccg cctttgcctt
    35041 ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata tgcagcacgg
    35101 tacgcgcacc agagagcttc gccagcaggc gcattcccgg cgtgcaaaac agcgttggca
    35161 ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc catcagtgga aagaaactgc
    35221 tgacggcaaa actgccgaga tgcaacaagc gtttcagggt gctcggctgt tttggcacgt
    35281 acagcgggca gcgccacacc gtggcggccc cttcttctcg tttgtagcgc caggcggaat
    35341 agttctcacc cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc cgcacctcat
    35401 gaccttgtgc cgccaaccat tccaccatct cgccggtgta tttgccgatg ccggttaact
    35461 ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc cggcacgctt
    35521 ctcagcgaga aaataggctc ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc
    35581 cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct cttcttccgc
    35641 tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc cggagaagtt
    35701 atcgtcataa aaatgctgcc agacaccgta aaactggcct gctgttatcg gcagacgcag
    35761 ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt ctttctgcac
    35821 gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt tgccaagcag
    35881 aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg tggagcgcac
    35941 tacagtggca aagtcttcct gacgtaaaaa catcattccc cccgaaagcg gtcttgattc
    36001 tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc gtgataccag
    36061 ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc cggtttgctg
    36121 gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg cgccagctcg
    36181 cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg ttgggtattc
    36241 tccagccaga cttcatgcgc cagctccatg acatgaatgc tcgccgccgc catatcatcg
    36301 acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc cggcgcattc
    36361 tgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta tcacatggga gttactcggg
    36421 tggaagttgt catgcggccc gtacaggttg gtcggcatga cagtgcggta atcgcgtcca
    36481 tactggcggt tgtaagattc gcacagtttg atcccggcga ttttggcaat ggcgtaaggc
    36541 tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc
    36601 agtttcgggt agatgcagga cgatccgaga aacatcagtt tgttcacgtc gttctgatgc
    36661 gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc cgccggatag
    36721 gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg gtcaatgcgt
    36781 tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca ggttcagttc atcgcgggta
    36841 cgtaatacca gctccacatc accgcgctgt tcgagctgcc gcttgatggc agaaccgacc
    36901 atcccacaat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac tccagcgcga
    36961 tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct tcgaggtcat
    37021 tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag cccagttttt
    37081 cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg aagtaacgcg
    37141 gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg
    37201 aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg atgcccagct
    37261 gtgctgccgc catttccaca aactgacgta cggagtactg aacgccggtc gcgataacga
    37321 aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag tctttggcgt
    37381 ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc agcccctggg
    37441 cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg
    37501 attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg cggtagttaa
    37561 cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg tagaacggcg
    37621 tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg gaagcctgat
    37681 agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac
    37741 ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca acgtggctca
    37801 ttgcgcctag gttgtacact tcatccggct gtacttcacg caaaatgcgc gtcaggttag
    37861 aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct
    37921 gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta ataccatgca
    37981 cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac
    38041 cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat ttattcagtt
    38101 tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt tgccggaagc
    38161 gatttaaaaa cactgcttcg tgcacccacg acggtgccgt c
//
LOCUS       DAJTYH010000028        38043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTYH010000028 DAJTYH010000000
VERSION     DAJTYH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..708)
                     /locus_tag="OO609_001419"
     CDS             complement(<1..708)
                     /locus_tag="OO609_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6632894.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERS"
     gene            complement(911..1147)
                     /gene="feoC"
                     /locus_tag="OO609_001420"
     CDS             complement(911..1147)
                     /gene="feoC"
                     /locus_tag="OO609_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6632895.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1147..3468)
                     /gene="feoB"
                     /locus_tag="OO609_001421"
     CDS             complement(1147..3468)
                     /gene="feoB"
                     /locus_tag="OO609_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6632896.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3485..3712)
                     /gene="feoA"
                     /locus_tag="OO609_001422"
     CDS             complement(3485..3712)
                     /gene="feoA"
                     /locus_tag="OO609_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6632897.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4150..6471)
                     /locus_tag="OO609_001423"
     CDS             complement(4150..6471)
                     /locus_tag="OO609_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6632898.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6568..7044)
                     /gene="greB"
                     /locus_tag="OO609_001424"
     CDS             complement(6568..7044)
                     /gene="greB"
                     /locus_tag="OO609_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6632899.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            7272..7991
                     /gene="ompR"
                     /locus_tag="OO609_001425"
     CDS             7272..7991
                     /gene="ompR"
                     /locus_tag="OO609_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6632900.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7988..9340
                     /gene="envZ"
                     /locus_tag="OO609_001426"
     CDS             7988..9340
                     /gene="envZ"
                     /locus_tag="OO609_001426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6632901.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9416..11038)
                     /gene="pckA"
                     /locus_tag="OO609_001427"
     CDS             complement(9416..11038)
                     /gene="pckA"
                     /locus_tag="OO609_001427"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6632902.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11417..13168
                     /locus_tag="OO609_001428"
     CDS             11417..13168
                     /locus_tag="OO609_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6632903.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            complement(13312..14181)
                     /gene="hslO"
                     /locus_tag="OO609_001429"
     CDS             complement(13312..14181)
                     /gene="hslO"
                     /locus_tag="OO609_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6632904.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(14206..14607)
                     /gene="hslR"
                     /locus_tag="OO609_001430"
     CDS             complement(14206..14607)
                     /gene="hslR"
                     /locus_tag="OO609_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6632905.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14618..15286)
                     /gene="yrfG"
                     /locus_tag="OO609_001431"
     CDS             complement(14618..15286)
                     /gene="yrfG"
                     /locus_tag="OO609_001431"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6632906.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15351..17486)
                     /gene="igaA"
                     /locus_tag="OO609_001432"
     CDS             complement(15351..17486)
                     /gene="igaA"
                     /locus_tag="OO609_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6632907.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            17806..18366
                     /gene="nudE"
                     /locus_tag="OO609_001433"
     CDS             17806..18366
                     /gene="nudE"
                     /locus_tag="OO609_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6632908.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18531..21083)
                     /gene="mrcA"
                     /locus_tag="OO609_001434"
     CDS             complement(18531..21083)
                     /gene="mrcA"
                     /locus_tag="OO609_001434"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6632909.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21203..21982
                     /gene="hofM"
                     /locus_tag="OO609_001435"
     CDS             21203..21982
                     /gene="hofM"
                     /locus_tag="OO609_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6632910.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21982..22521
                     /gene="hofN"
                     /locus_tag="OO609_001436"
     CDS             21982..22521
                     /gene="hofN"
                     /locus_tag="OO609_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6632911.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22505..22945
                     /gene="hofO"
                     /locus_tag="OO609_001437"
     CDS             22505..22945
                     /gene="hofO"
                     /locus_tag="OO609_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6632912.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            22935..23341
                     /gene="hofP"
                     /locus_tag="OO609_001438"
                     /pseudo
     CDS             22935..23341
                     /gene="hofP"
                     /locus_tag="OO609_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            23253..24491
                     /gene="hofQ"
                     /locus_tag="OO609_001439"
     CDS             23253..24491
                     /gene="hofQ"
                     /locus_tag="OO609_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6632913.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24895..25416
                     /gene="aroK"
                     /locus_tag="OO609_001440"
     CDS             24895..25416
                     /gene="aroK"
                     /locus_tag="OO609_001440"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6632914.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25473..26561
                     /gene="aroB"
                     /locus_tag="OO609_001441"
     CDS             25473..26561
                     /gene="aroB"
                     /locus_tag="OO609_001441"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6632915.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26653..27945
                     /gene="damX"
                     /locus_tag="OO609_001442"
     CDS             26653..27945
                     /gene="damX"
                     /locus_tag="OO609_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6632916.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            28052..28888
                     /gene="dam"
                     /locus_tag="OO609_001443"
     CDS             28052..28888
                     /gene="dam"
                     /locus_tag="OO609_001443"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6632917.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28906..29583
                     /gene="rpe"
                     /locus_tag="OO609_001444"
     CDS             28906..29583
                     /gene="rpe"
                     /locus_tag="OO609_001444"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6632918.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29576..30334
                     /gene="gph"
                     /locus_tag="OO609_001445"
     CDS             29576..30334
                     /gene="gph"
                     /locus_tag="OO609_001445"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6632919.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30327..31331
                     /gene="trpS"
                     /locus_tag="OO609_001446"
     CDS             30327..31331
                     /gene="trpS"
                     /locus_tag="OO609_001446"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6632920.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31601..32506
                     /locus_tag="OO609_001447"
     CDS             31601..32506
                     /locus_tag="OO609_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632921.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32523..32885
                     /locus_tag="OO609_001448"
     CDS             32523..32885
                     /locus_tag="OO609_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6632922.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            32969..34132
                     /locus_tag="OO609_001449"
     CDS             32969..34132
                     /locus_tag="OO609_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6632923.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            34133..35359
                     /locus_tag="OO609_001450"
     CDS             34133..35359
                     /locus_tag="OO609_001450"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6632924.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            35356..36234
                     /locus_tag="OO609_001451"
     CDS             35356..36234
                     /locus_tag="OO609_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6632925.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            36245..36598
                     /locus_tag="OO609_001452"
     CDS             36245..36598
                     /locus_tag="OO609_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6632926.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36610..37914
                     /locus_tag="OO609_001453"
     CDS             36610..37914
                     /locus_tag="OO609_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6632927.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37926..>38043
                     /locus_tag="OO609_001454"
     CDS             37926..>38043
                     /locus_tag="OO609_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR6632928.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGD"
BASE COUNT         8875 a         9588 c        10389 g         9191 t
ORIGIN      
        1 tgaacgttcg gcaacgccgt cgataaaatc gttaaaacgt acagcgtcgc cagtttgcag
       61 tatgtaatta aacagccctt tgatttgtct gtcattagcg tatccactac ttaataagca
      121 ggccatttgc tctaccagcc ccatcagatc gcgttgacga atatgttttt gaattaactc
      181 cagcagcgcc atacgtcggt gctgcatgat ttcatcatca ggcatgacgg tgacatcaat
      241 cagcggaaat gcggaggcat aaagctgcct tgccagtttg ggatcggcga aacaatccag
      301 ccaacacagc gaatagggat aggggctttc aataccgtgg taaaacaaca atggcaccac
      361 catcggcaac gttttgtatc cagcatcaag gtgattttgc attgcggcaa tagcgtaacg
      421 catcatgcga aatgcgatca gtttgtttga ggtgctttga tgttcaatca gacaatagat
      481 gtatcctggt ccttgttccg ttttcaccga ccacagcaca tcggaatagc tttcacgcag
      541 atcgtcatca ataaagctcc tcgactccag cttgagggtt ttcatatcac agagcgcgtg
      601 aatgggtgcc ggtaaatgaa acgcaagaaa atcgcgtgcc gtctccggtt ggcgtaaaaa
      661 gagtttgaac agcgcatcat gtggggtgga actctgcttt ttactcatgg cgttccttca
      721 cctgttgttt gaatgccgcg actctacgcg ccactccaca tctgtgcatt agcgagtttt
      781 catccctgcg agacgctttc agaggaactt acgttgcgct gcaagaattg catttttatg
      841 gaaagccgca cgcaaaaaag cccggatgca aacgcatacc gggcttttca aaacaggcga
      901 tggagtatgg ttaacgcagc gcccaccact cgcgcagaca ggctttgcct tccgggcagc
      961 ttttacaact gccagagagg cagccgtcag gttcttcctg aatccgcacg gctttgccca
     1021 tgctttccag ctgttgcagc atggcgttaa tcattggctg cggagtgttc aatgtctggc
     1081 ttatctgggt cgcttccata cggccccgta acgccagcaa atcacgtacc tgaataagtg
     1141 aagccattaa tggcaatcac cggtggtgct ggctgcgcag caactgctta ccgacttgcg
     1201 ggttgccagc agttcgatat ccacccggct acgcgcgcgg cgcagcagac cgatagctac
     1261 gatgttaaac aggattaccg ccagaatgca caccaggctg taagttgggt gctgactgta
     1321 gctggcgacc tgatagaaca atgttgccag tgagtaagcg atattcagcc cccacaggat
     1381 ggagaagccc atccagccac ggcttgattc acgggcgatg gctcccatca ccgagataca
     1441 tggtacatac agcaggacga aaatcaggta gctgtatgcg gcagctgcgc taccgaattt
     1501 ctgatccatc acgcccatcg ccccggtacc catttcaccg ttgcctttgc tagcttcaat
     1561 ggggttcatc agtacgctaa ggctgaaggt gtctttcagg ctctgccagg tttcatctac
     1621 cgcactgaac agctcttcac cgaggttgaa ttccgccgga ttgaactctt cgtcctgaat
     1681 attttctgcg gtgtagaggg tgttgagcgt acccaccacc acttcttttg ccattgcgcc
     1741 cgtaaacagg ccaaccgttg cctgccagtt atcttcatgt acgccaatcg gcttgaagac
     1801 aggggttatc acccggctga cggaagccag cgccgagtcg ttgatgttat cgacgatttt
     1861 cccgctcaac gagaagctgt tgaaagcgct caggaaaatg ctgacgatga taatcacttt
     1921 accagcacgc agaacgaagc ccttcagacg ctgccaggtc tggataatca ggcttttaac
     1981 gtgtggtaca tggtagaccg gcagctccat gacaaacggc gtcgcttcac cgcgcatgat
     2041 ggtgtacttg agcatcaggc cagtcagcac cgccatcacg ataccgagca tatacagcga
     2101 gaaaaccgcc agggcaccgt tctgcccgaa gaaggcagcc gcgaatactg cgaagatagc
     2161 cagacgcgcg ccgcaggaca taaacggtgc catcatgatg gtcatcagac gttcacgcgg
     2221 tgcatcaagc gtacgtgcgc ccattaccga cggtacgtta caaccgaaac cgacgatcag
     2281 cggcacaaag gatttccccg gcaagcccag cgcctgcatc aggcggtcca tcacaaacgc
     2341 tgcacgcgcc atatagccgg agtcctcaag gaaggagagg aacaggtaca tcatgccgat
     2401 ctgcggcacc agtggcagca cggtgttaat accgccaccc aggccctggg cgaggaagat
     2461 agtcagccag tccgggaagt ggagcgtgta gccaatccat tgaataccat gcacaaatag
     2521 cgccacggag ccgacgttaa acagcggctg taacgccccg ccgatgttga tagccagcag
     2581 gaacatcagg tacatcacaa agaggaaaat cggcagaccg aggaaacggt tgagcacgat
     2641 tttatctacc gcagtggtga aacggctggg ttctgccgtc agggtgttgc ttaccacatc
     2701 acagatggca gcaatgcact ggtaacgcgc atcggcaatg tgcagcgccg gatcatccat
     2761 ttcattacgc agacgggcga gggtggcatc cagatgctgc gacgcttcac cggcgtaggc
     2821 gcggctgtag atatcgcctt ccagcatttg caggcccagc cagcgacgtt gtttcagcgg
     2881 gatgtcggaa ggcatcactt ttgccagtga atctgcttcg ttgagtagcg gctgtgcgta
     2941 atgcaccagt tccacattct cgttagcttt atagcgatca atcgccagct tgagcgcttc
     3001 aataccgcga ccacgggttg aaaccagcgg gatcaccggg cagccaagac gtgccgatag
     3061 agcatcaatt tcaatacgaa tattttgctt ctcggcaatg tcgagcatgt tcagcgccac
     3121 gatgcaggga atgccgagtt ccagcagttg cagcgtcagg tacaggttac gctcaaggtt
     3181 agacgcatcc accacgttaa tcagcaggtc ggcgtcgcca ctcaaaatgt agtgacaggc
     3241 gatttgctca tcgagcgagg tctgcgatga gatggtggtc agagaatagg tgccgggcag
     3301 gtccaccagc gtgacctgat gatcggtggt ggagaattgc ccttctttac gttcgacggt
     3361 aacgccagcc cagttaccta cacgctgacg tgcgccagtg agctggttaa ataaggttgt
     3421 cttgccagaa tttggattac caattaagcc aatggttaat tttttcattg ttgttatcac
     3481 cgtattaaca ggaaaccgct tccacttcta ataaggccag atcttttttg cgtaatacca
     3541 ggctcacacg acgggtttcg atatgaatgg ggtcgccgag tggagcgacg cgcaccacat
     3601 caaaagagga gccaggtaac atgcctagag aaagcagttt ttggcgatat gccgggctga
     3661 tttcacggga aaagccagtg attttccacg cagtatctgg agtgtattgc ataggtgcct
     3721 acttgtttct cattaactgg ataactacct catggggcgt ttgaccgcga catgagacca
     3781 gatgatttgc caacaaagga gagattaaat aatcaacaac acaatgataa tgagaatggt
     3841 ttctatcagc aatacttaaa atgtgtgcga atgttggttt gaataataat ctgactcaat
     3901 gttgatgtgg ctcaaggtta tatcgcagcg aaaatataat tcgaatagtg agttacccgg
     3961 actggctaat gtttaattaa ggtttcagtt gttaatgaag tgataaatat taacggagta
     4021 tattgtgagt ttggctgtgg aataaaagtg ccggagatat tctccggcac ttttatgtca
     4081 ggcaaagcgt tcgttgccag atgcggcgtg aacgccttat cgcgcaggtt taggggccag
     4141 tccgggcgtt taacgttttt tacccatcgc tgccgccagc gcatccatca tcgcgctatt
     4201 accggcaggc tgcgcttcac gaccgcgtgg tttggctgcc gggcggttgt tttgcgggcg
     4261 ttcattaccg ccgccgcgac gagagttggt ttcgccaggc tgctcgtcca gacgcatagt
     4321 cagggcgata cgtttacgct gaagatccac ttccagcact ttcaccttca caatgtcgcc
     4381 cgctttcacc acggtatgcg gatcttccac aaacttgttc gacaatgaag agatgtgaac
     4441 caggccgtcc tgatgcacgc caatatcgac aaacgcgcca aagttggtga cgttggtcac
     4501 tgcgccttcg aggatcatac ccggttgcag gtcgttcatt gtctcgacgc catcggcaaa
     4561 ctgagcggtt ttaaattccg gacgcggatc gcgacccggt ttttccagct ctttgatgat
     4621 gtcagttact gtcggcacac cgaatttttc atcagtaaag tcagacgctt tcaggttacg
     4681 cagttcgctg ctgttaccca tcagatcttt cagtgcctgc tgtgttgctg ccagaatgcg
     4741 ttccaccacc ggataggctt ccggatgaac ggtagaggcg tccagcgggt tatcaccgtg
     4801 gttaatgcgc aggaagcccg cgcactgctc gaaggctttc ggccccagac ggctgacttt
     4861 cagcagttgc tgacggttct ggaactggcc gttctcatcg cgccaggcca cgatgttttg
     4921 cgccatcatg cgcgtcaggc ccgccacgcg ggttaatagc ggaacagaag cggtgttgag
     4981 atcgacgcca acggcgttta cgcagtcttc tactactgca tccagtttgc gggccagttg
     5041 cgtctggctg acgtcatgct gatactgacc tacgccgata gatttcggat cgattttcac
     5101 cagctccgcc agcggatcct gcaaacggcg ggcgatagat accgcaccac gcagcgaaac
     5161 gtcgagatcc gggaactcct gcgctgccag ctcggaagct gagtaaaccg acgcgccagc
     5221 ttcgctgacg atcactttct gtgcggtcac tttcgggaac tgcttctgca cgtcgagata
     5281 gaaacgttca gtttcgcggg aagctgtacc gttgccgatc gccaccagtt caacgttatg
     5341 tttttcacac aaggcagcaa cggtcatcgc tgcttttgcg gcttgtccgg tgtgcgggta
     5401 aatggtatcg gtcgccacca gtttgccagt ggcatcgacc accgccactt ttaccccggt
     5461 acgcagaccc ggatcgaggc ccatcgttgc acgcagtccg gcaggggccg ccatcagcag
     5521 atcgtgcagg ttacgggcaa agacgttgat tgcttcatct tccgcgcgtt cgcgcacggt
     5581 acccatcagt tcggtttcca gatgcatcag caccttgatg cgccaggtcc agctcactac
     5641 gcttttgcgc cagctatccg ccggggcatt gttcaggcgc aggccaaggt gatccatgat
     5701 gatttgctcg caatagctct ctttgggcgg ctcatcgaac tgcggatcgg cattcagcga
     5761 aagctggagt acgccttcgt tacgcccacg gaacatcgcc agcgcgcggt gagaaggcac
     5821 cgtggacaac ggttcgtgat gatcgaaata gtcgcggaat ttcgcccctt cctcttcttt
     5881 accgctcacc accgtagaaa ccaaatgcgc gttcttccac agataatcac gcactttcgc
     5941 cagcagcgtg gcatcttcgg caaaccgttc catcaggata tagcgcgcgc catccagcgc
     6001 ggctttggta tctgccacgc ctttatcggc atcaacatat tgtgcagcgg cgacttctgg
     6061 cgtgtgtgac ggatcgctcc acagcaggtc agccaacggc tcaagccctg cttcaatggc
     6121 gatttgcccg cgggtgcggc gtttaggttt gtagggcagg tagaggtctt cgagttcggt
     6181 tttgcttagg gtggcgttga tggccttcgc cagatcatcg gtgagtttgc cttgctcgga
     6241 aatggatttg aggatcgcct gacgtctctc ttccagctcg cgcagatagc tcagacgcgt
     6301 ttccagatta cgcagctgcg tgtcatccag accgccggtg atttccttac gataacgtgc
     6361 gataaacggc acggtattcc cttcgtcaag caggcgaacg gcagcgtcaa cctgttccgg
     6421 gcgcgcctga atttcacccg caataatgcg gcagaacgaa tcattcatca tggtttgagt
     6481 tcatcttttc ggatcaaaaa tcaggggata gttatacgga ctggctggca aaaatgccag
     6541 ccatcggcag gaggttaaga ctcttcctta cggtttcacg tactcgatag cattaacata
     6601 ccagttcgct tccccggcag gggtattcac caccgccaga tcgccgactt cttttttcag
     6661 caatgcgcgg gccatcgggg aatcgataga gatgtaatct ttacggccaa aaatttcatc
     6721 gtagccgaca atacggaaac ggtgagtcac gccatcgtcg ttttcaatct ccacccacgc
     6781 gccaaaaaag actttgcctt cctgctgagg ggagtaatcg acgattttga gattttccag
     6841 gcatttagtg agatagcgca cgcgacggtc gatttcacgc agacgctttt tattatactg
     6901 atagtcagca ttttcgctgc ggtcgcccag acttgcggcc caggtcacct tttttgtgac
     6961 ctccgggcgt tcttcacgcc agagataatt aagctcttgt ttgagttttt catacccttc
     7021 ccgggtaacc aggggcgttt tcatctcgtt gattcccttt gtctgtttga taatgcgcac
     7081 attgggtata acgtgatcat atcaacagaa tcaataatgt ttcgccgaat aaattgtata
     7141 cttaagctgc tgtttaatat gctttgtaac aatttaggct gaaattcata ccagatttag
     7201 ctggtgacga acgtgagctt ttttaagaat acacgcttac aaattgttgc gaacctttgg
     7261 gagtacaaac aatgcaagag aactacaaga ttctggtggt cgatgacgac atgcgcctgc
     7321 gtgcgctgct ggaacgttat ctcaccgaac aaggcttcca ggttcgaagc gtcgctaatg
     7381 cagaacagat ggatcgcctg ctgactcgtg aatctttcca tcttatggta ctggatttaa
     7441 tgttacctgg tgaagatggc ttgtcgattt gccgacgtct tcgtagtcag agcaacccga
     7501 tgccgatcat tatggtgacg gcgaaagggg aagaagtgga ccgtatcgta ggcctggaga
     7561 ttggcgctga cgactacatt ccaaaaccgt ttaacccgcg tgaactgctg gcccgtattc
     7621 gtgcggtgct gcgtcgtcag gcgaacgaac tgccaggcgc accgtcacag gaagaggcgg
     7681 taattgcttt cggtaagttc aaacttaacc tcggtacgcg cgaaatgttc cgcgaagatg
     7741 agccgatgcc gctcaccagc ggtgagtttg cggtactgaa ggcactggtc agccatccgc
     7801 gtgagccgct ctcccgcgat aagctgatga accttgcccg tggtcgtgaa tattccgcaa
     7861 tggaacgctc catcgacgtg cagatttcgc gtctgcgccg catggtagaa gaagatccag
     7921 cgcatccgcg ttacattcag accgtttggg gcctgggcta cgtctttgta ccggacggct
     7981 ctaaagcatg aggcgattgc gcttctcgcc acgaagttca tttgcccgta cgttattgct
     8041 catcgtcacc ttgctgttcg ccagcctggt gacgacttat ctggtggtgc tgaacttcgc
     8101 gattttgccg agcctccagc agtttaataa agtcctcgcg tacgaagtgc gtatgttgat
     8161 gaccgacaaa ctgcaactgg aggacggcac gcagttggtt gtgcctcccg ctttccgtcg
     8221 ggagatctac cgtgagctgg ggatctctct ctactccaac gaggctgccg aagaggcagg
     8281 tctgcgttgg gcgcaacact atgaattctt aagccatcag atggcacagc aactgggcgg
     8341 cccgacggaa gtgcgcgttg aggtcaacaa aagttcgcct gtcgtctggc tgaaaacctg
     8401 gctgtcgccc aatatctggg tacgcgtgcc gctgaccgaa attcatcagg gcgatttctc
     8461 tccgctgttc cgctatacgc tggcgattat gctattggcg ataggcgggg cgtggctgtt
     8521 tattcgtatc cagaaccgac cgttggtcga tctcgaacac gcagccttgc aggttggtaa
     8581 agggattatt ccgccgccgc tgcgtgaata tggcgcttcg gaggtgcgtt ccgttacccg
     8641 tgcctttaac catatggcgg ctggtgttaa gcaactggcg gatgaccgca cgctgctgat
     8701 ggcgggggta agtcacgact tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat
     8761 gatgagcgag caggatggct atctggcaga atcgatcaat aaagatatcg aagagtgcaa
     8821 cgccatcatt gagcagttta tcgactacct gcgcaccggg caggagatgc cgatggaaat
     8881 ggcggatctt aatgcagtgc tcggtgaggt gattgctgcc gaaagtggct atgagcggga
     8941 aattgaaacc gcgctttacc ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa
     9001 acgcgcggtg gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct ggatcaaagt
     9061 cagcagcgga acggagccga atcgcgcctg gttccaggtg gaagatgacg gtccgggaat
     9121 tgcgccggaa caacgtaagc acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac
     9181 cattagcggc acgggattag ggctggcaat tgtgcagcgt atcgtggata accataacgg
     9241 gatgctggag cttggcacca gcgagcgggg cgggctttcc attcgcgcct ggctgccagt
     9301 gccggtaacg cgggcgcagg gcacgacaaa agaagggtaa ataaacggga ggcgaaggtg
     9361 cctcccgttt tgctttctat aagatactgg atagatattc tccagcttca aatcattaca
     9421 gtttcggacc agccgctacc agcgcggcac ccgcaggggt gtcggtgtat ttatcgaagt
     9481 tgtcgataaa cagtttcgcc agggtttcgg ctttttcctg ccactgttcc ggagaagcgt
     9541 aggtgttacg cggatcgaga atcttcgtgt ctacgcccgg cagttcggtt gggatcgcca
     9601 ggttaaacat cggcagagtg aaggtttctg cattatccag cgaaccgttg aggatggcgt
     9661 cgataatggc gcgggtatct ttaatcgaga tacgtttgcc agtgccgttc cagccagtgt
     9721 taaccagata agcctgcgcg cccgccgcct gcatacgttt caccagcact tctgcgtact
     9781 gagtcgggtg cagcgacagg aacgccgcgc cgaagcaagc ggagaaggtt ggcgtcggtt
     9841 cggtgatgcc acgctcagta ccggccagtt tggcggtgaa gccagagagg aagtgatact
     9901 gggtttgatc ggcagtcagg cgagaaaccg gcggcaacac gccgaaagca tcagcagtca
     9961 ggaagataac cttagtcgcg tggcccgctt tggaaaccgg cttaacgatg ttatcgatgt
    10021 gatagattgg ataagaaacg cgggtgttct cggtttttga accatcatca aagtcgatag
    10081 tgccatcttc acgcacggtg acgttttcca gcagcgcatc acgacggata gcgttgtaga
    10141 tttcaggttc cgcttctttc gacagcttga tagtttttgc gtagcagccg ccttcgaaat
    10201 taaacacgcc gtcatcgtcc cagccgtgtt cgtcatcgcc aatcaggcgg cgtttcgggt
    10261 cggtggaaag ggtggtttta ccggtgccgg aaaggccgaa gaacaccgca acatcgcctt
    10321 tctcgccaac gttggcggag cagtgcatag aagcgatacc tttcagcggc agcaggtagt
    10381 tcatcatcga gaacatccct ttcttcattt cgccgccgta ccaggtgccg ccaatcagct
    10441 gcatgcgctc ggtcaggtta aacgccacga agttttcgga gttcagaccc tgttctttcc
    10501 actgcgggtt agtgcacttc gcgccgttca taacgataaa gtctggtttg aaacctgcca
    10561 gctcttcatc gctcgggcga ataaacatgt ttttgacaaa atgcgcctgc caggccactt
    10621 cggtgatgaa acggacggaa agacgagtat ccgggttcgc accacagaaa gcgtcgacaa
    10681 cgaacagacg tttgccggaa agctgcctgg tcaccaggcc tttcagatgc tgccaggttt
    10741 ccggagagag aggtttgttg tcgttcttac ctttgccttt gtctgcccac cagaaagtat
    10801 cgcgagtggt atcgtcacgg acgatatact tatcttttgg tgaacgaccg gtgaagatcc
    10861 cggtatcgac ggcaacggca cccagattag ttaacacccc gcgctcataa cctgtcaggc
    10921 tcggatcgag ctcttcctga tacagcaggt cgtagcttgg gttgtaaacg atatcatgta
    10981 cgtcactgat accataagcc tcgagttctt gcgggggcaa accattgtta acgcgcattt
    11041 cactgctcct tagccaatat gtattgcctg aatagtaaag tctttttggg ggtgttaacc
    11101 gcgacaaggc tcatagattt acgtatctgg agaaattcat atttgacaaa aaactccgtg
    11161 attcctgtca cgaaacggtt gctattatcg cagaaatagc attttaggtg tggaaaatgt
    11221 tctcaaaagg ttaaatcttg gtttatatgg cgggaatatt gtgagtggaa tcgcattcct
    11281 gaatgaaatt gatgcaaatt caaccggtaa agtatcgatt caccacgcta actttttttg
    11341 attattttct gaaaaaagca aaatactact gcccggatgc gataattaac ccaccttttg
    11401 attctggata accaacatgg ataacgttga actttcaccg gcaacacgct gggggatgat
    11461 tgctaccgga ttacttcagg ggatgattgc taccggatta cttcaggggc tggtttgcta
    11521 cctgcttatc gcctggcttg ccggaaaaaa tcacagttgg attgtttatg gcgtgcctgc
    11581 gaccgtcgcg ttttcatccg ttttactctt ctccgtgatc tcttttaaac agaagcgcct
    11641 ttggggatgg ctggcgctgg tgtttattgc cacgacaggg atgagcggtt ggctgaagtg
    11701 gcaaactgat ggcatgaacc cctggagagc tgaaaaggcg atttgggatt ttggctgcta
    11761 tctgctgttg atggcaatgt tgttgctacc gtggatacaa caaagccttc gtatccgcaa
    11821 tggcagtagc cgctatagct acttttacca gtcagtatgg cataacgtac ttatattact
    11881 ggtgattttt ctctccaatg gcctgacgtg gctggtgctt ttactgtggg gtgagttgtt
    11941 taaacttgtt ggcatcacgt ttttcaatac gctctttttt gcaaccgact ggtttatgta
    12001 tcttacattt ggtctggtta ccgcactggc ggtgatcctc gcgcgtacac agtcacgttt
    12061 aatcgactct attcaaaagt tgttcacgct aatcgccacg gggttgctgc cgttagtatc
    12121 attgctaacc ctgatgttta tcatcaccct gccgtttacg ggcctgagcg cgatttctcg
    12181 ccacatctcc gccgccgggt tgctgttaac gctggccttt ttgcaattga tcttaatggc
    12241 tattgtccgc gatccgcaaa aagcgtcact tccctggacg gggccgttgc gttgcctgat
    12301 taaaaccgct ttgctggttg ctccgctgta tgtgttcgtc gccacctggg ggttatggct
    12361 gcgggtcgct cagtacggct ggactgtcga ccgcttgcag ggcgcgctgg cggagctggt
    12421 gttactggtg tggtcgctgg ggtattttgt cagtatcgtc tggcgtaatg ggcaaaatcc
    12481 ccttgttctt cagggcaaag tgaaccttgc ggtttcgtta ttggtgttgg tgatactggt
    12541 gcttcttaat tcgccggtgc tggacagtat gcgtattagc gtgaacagtc atatggcgcg
    12601 ttatcagagc ggcaaaaaca cgccagacca ggtaactatc tacatgctcg aacagagcgg
    12661 tcgctatgga cgtgcggcgc ttgagtcgct gaaaagcgat gccgggttta tgaaagaccc
    12721 gaaacgcgcg cgggatctgc tgatggcgtt agatggagag caacatcttc aggaacaggt
    12781 atcggaaaaa gtattcgccg aaaatgtgtt aattgcccct ggttctgtta aaccggatgc
    12841 gacattctgg tcggccttaa tccaggatcg ctataacgtg atgacctgta ttgaaaaaga
    12901 cgcctgcgta ctggtcgagc aagatctgaa tagtgatggt caggcggagc ggatcctgtt
    12961 tgcttttaat gatgacagag tcattgtcta tggctttgac tcagacagaa aagaatggga
    13021 cgcgcttgat atgagtttac ttccgaacga aataacgaaa gaaaaattac tcacagctgc
    13081 gaaggatgga aaactgggga cgaggccgaa agcgtggcgc gatctgacgg tagatggtga
    13141 aacgctggaa ataaacttaa gtaagtgaca acgcttttat cggatacgac gctgtatctg
    13201 accaaaataa tttgcgattt atttcatagg cgctggtaag cgcagcacat caggccatta
    13261 agtcgtaaaa tccggcagag ccctctctgc cggacatact cattaatgaa cttacggatc
    13321 tgccggagac gcgttgttgc ggatttcagc aatatccatc gcattgaaca gatagtggtt
    13381 accgcagtaa tcacaatgca tgtcaatttc gccatcttcc gccaggatgc tatcaacttc
    13441 ttcatcaggc agcgttttca gcgcatcggc gcaacgttca cgcgagcagg tgcatttgaa
    13501 ctccacatcc tgcggatcgt aaaccgtcac ctcttcttcg tgatacaaac gccacaacac
    13561 ttcgtttgcc ggtaaggtca gcagttcttc ggttttgatg gtttcggtta gcgtcgccag
    13621 gtggtcaaag tcgtcctgtt gggtattttg cgcaggcatt acctgcaaca acataccgcc
    13681 tgcagccggt ttgccgtcta cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc
    13741 agaacgcata aagtaatctt ctaggcaggc cgccagggta tcaccttcca gaccaactac
    13801 gccctgatag cgttcgcctt cgctcggggt aatggtgatc accacgtaac cattgccgac
    13861 cagcgttttc aggtcggcat tttctggaat ttcgccctgc acgcgcgcca caccgcgcat
    13921 ttgctggtta ttgttaccgt taataaccgc cagattcatc ggaccgtcgc cctgcagctg
    13981 tacggtgata tcaccatcaa acttcagcgt agcggttaac aggctggtcg caaccagcag
    14041 ttctgccagc acgtttttaa cgggctgcgg ataatcgtgg ttctcaagga tctgttgcag
    14101 ggtttccgaa acggttacca gttcgccgcg aacggcaaaa ttttcaaaca gatagcgatg
    14161 taattggtca tgttgcggca taatcatctc tcttgcaggt gacagttatt cactgtcgcc
    14221 gtgtttaaat cgtaacaggt cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg
    14281 catggttaag gcattaagtt tacgtgccat cgccattttt tcgcgtttct ctacactttc
    14341 cgcagtctct tcatacagca aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc
    14401 ctttacaatc accgtgcgtt cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc
    14461 gacgattttg ctcggcttgc tgcgctgccc gttgtaatgc accttaccgc cttcaatcat
    14521 ttcacgggcc agcgcgcggg ttttataaaa acgggcagcc catagccatt tatccagtcg
    14581 aacctcaaca gcaggtttct ctttcatggc gtctccttca cattagcgag gggatcaggc
    14641 ggcggtagtc attcagtgac ggatggcgtt gatactgttt ctcggcaatc ccggaatcag
    14701 gattagtcac gccgaggcag taacgaatac caaattgcgc ggcagcatcg agaatcgctt
    14761 cgctgtcatc aataaacagc gttctttcag ctttcagacc cgtagcttcg gccaccgcat
    14821 gccataaccg ctgatcctct ttcggataac caaatgtgtg ggtggaaagt aataaatcaa
    14881 ggtgtgcgtc cagacccgta tgctcaagtt ttaccgccag gttgtgcgga tgagcattgg
    14941 tgagcaaaat tcgctgctta ccgctggctt tcagtgcctc aagaaacgga atggtatctt
    15001 cacgcagtac ggcgcgcggt cccatctcgg tggtcatcgc acagatatcc agacccagtt
    15061 gctcactcca gtaatcaaga cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct
    15121 ggcgcatata ttccatcgct tcctgtggcg taaccccgtt tttcgcgccc catgtttcag
    15181 gcaccagctt ttgccagaaa tagttatcga aggcgaggtc gagcaacgtg ccgtccatat
    15241 ccagcagaac ggtatctacg tcctgccagg caatgttgat atgcatgagg gaaatctcca
    15301 gagtgaagca atttgcgcga cagggtagca taacctgccg cgcaaacgtg ttattcgata
    15361 aggctttctg aaggggtgat cagttgcggg ttcaggcagc tttcataata ggcctgaatc
    15421 tccatcatgc gagtgcgatg acgctggtaa cgacgccagg cctgtacgcc attgtaaatg
    15481 gcgctaccca gcatcgtcag tagcagcaat gtggtgctga gatagcgcca caggccggaa
    15541 cgatccggga tcggatgaag gccaatatgc tgcgtaccat tggcgtcagt gaagattttt
    15601 gtgacgatac cttcggcgtt aaacggcgta tgcatcagca tttgtgccag tttctggaaa
    15661 gcgttccact gttcttgcgg cgggtagtcg taaagtgatg ccgaaggcca gggctgatca
    15721 acaaaatcgc tgccttcgtc gctgacaatc aggaatccgc cgggggccgg actgtttagt
    15781 gattgtgccg ctcgcgccgt ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg
    15841 ttatccagcg attcagcact caccgggcgt aataacacat tcacgccatc gagcttcccg
    15901 gcgttggcgc gttttaccag cgcgtcccag tctttactgt tgccgagatt gaccagcgca
    15961 tttttcagtc gcacacagtc atctttggca gaacataaat ccgctgtctt cagtacaatg
    16021 tcgccaaaat catcaagcaa taccatgccg gatttttgaa tcgctgaacg taatgaggca
    16081 ctgacgcgag attcatcttc cggtttaggg tgcagctggc gattaactgc ttcagtcaat
    16141 gccgtcgctt tgttgaccag ttcagattct ggtaatggta atgagcgggc gtcattccag
    16201 atgatctgcg agcagtcaaa cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg
    16261 gaagtgcgaa tattacacat tcccgtaccg ctaatacgca atgtatcgcc tacccgcacg
    16321 ccagcgtcag ccagttgttt tacgctggtg gcttcaatgg tctgcgcgcc tttcatccat
    16381 gagagagtga atttcagcgg catatccagc gggatccaga ataacagcat aaacagcact
    16441 agcagcgagc ccgcagcgat aatcgtactg cgcagccagt gctgtaacgg aaagtttttt
    16501 acttcatcat gcagcgaaag atatcgcccc tgacgcacta cgtggcggtc gagatagata
    16561 tcaatatcgg tttgttgacc gagatcctga gcaatgtatg gctgccagtg tgcgggatag
    16621 accaggtcga taataccgag tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac
    16681 aatccccaac gacggggtgt accgcgcaga caatgaattt ctcgcaggga ggattttgcc
    16741 gggggcgcga atagccccca cagacctgcg cccagcagca gtaatgcgcc gcctgccagc
    16801 cacggaacaa atacatccgg ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg
    16861 atcagcaacg cttcacgcag cccgcgcgga cgactcagcg catattcttc atgcgtttct
    16921 ttgcgaatat tgagtagctc gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg
    16981 gacggaacag gttgcaaggc atagccgcgc gtttcctgca tgtactcctg cagcgtatga
    17041 ccgttgagtg aaataaccaa cggcagcgaa tcggtatgaa tcagttcaac ggtattctca
    17101 tcgttgatgt actgttccca gaaggggggc aggtggactt ctaccgaatc gaggtagtaa
    17161 cgccatttat tcggatcgtc ggtagagatg ccgtaacgtg tgatagcgtg tgtcagcatc
    17221 atgacgttgt tgctttcggc attcagcgcc agagagatcg gtgccgcgct agcttccgtt
    17281 gggccaggca cctgtaatac ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt
    17341 tcttcaggtg tgagtttacg cgtttgcgca tccgcgaagg cgttggtcca gggcagctga
    17401 cgccgtctgg atcgcacttt tatcagccat cccgcaagta gtgagcaggc cagcaaagca
    17461 gctaaaaaaa tcacaatggt gctcatgctt tccccatctt acttagtctg tcaggcgtgg
    17521 tcagtcgcac cggacacaat acatgattct gcggttggct atcggcaagc gtggagctta
    17581 acttgagcat cattaagcac atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg
    17641 gaatatcagc aaattcctgg agttatttca acctattgac ttttaatttg atttgaggac
    17701 gattatttat taagttacat aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt
    17761 ccgatatcgc acaataaccg ctgatttcac agcatagatc ccacgatgaa caaatcatta
    17821 caaaaaccca ccattctgaa tgttgaaact gtagcccgtt cccgactgtt taccgtcgag
    17881 agcgtggatc tggagttcag caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc
    17941 aaccgggaag cagtgatgat tgtgccgatt gtggacgatc acctgatcct gatccgcgaa
    18001 tacgcagtgg gaactgaatc ctacgaatta ggtttttcga aaggattaat tgatccgggt
    18061 gaaagcgtct acgaagccgc taaccgcgag ctaaaagaag aggttggatt tggtgcgaac
    18121 gatctgactt ttttgaagaa gctcagcatg gcaccgtctt acttttccag caaaatgaat
    18181 atcgtggtcg cgcaagatct ctacccggaa tcgctggaag gcgatgagcc agagccgcta
    18241 ccacaggtgc gctggccgct ggcgcatatg atggatttgc tggaagaccc tgacttcaac
    18301 gaagcgcgta atgtcagcgc gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga
    18361 gtgtaaatgc gtgaagtttg cctgatgcac aacgcttatc aggcttatgg ggttcctgca
    18421 atatattgat ttttcacgat tttgttagcc ggataacgcg ttcacccgca tccggcataa
    18481 acaaatgcac tttgtcagcc aactgaaaag gcgccgaagc gcctttttaa tcagaacaat
    18541 tcctgtgcct caccattatc gataatggtc gttcccacct cgtgcaccgc ctgttgtgtc
    18601 ggctgcgtac cttcgatgaa atactcttcg cggctgttgc caccattagc taactgcccg
    18661 gtgctgcgat cgatattcac cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc
    18721 acaccttcaa gaacggcttt catataagcg tcccatgcag gctgggcact cttggcaccg
    18781 ccttcgtaac ctgagatctg atctttaatc gctccggaag ccgttgtatg accgagatta
    18841 cgacggtgat catcaaagcc aatccagacc gaggtcacaa cgcccggacc gtaacccgag
    18901 aaccacgcat ctttcgaact gttagtggta ccggttttcc cgccgatatc gcgacgctgc
    18961 aaatcacgac ctgcacgcca gccggtaccc tgccagcctg gttcaccaaa gatattggtg
    19021 ttcaaagcac tcttaatcag gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc
    19081 tgcgcgccag tcttcgccac taacgcctga tttgcctgct ccagctgcgg cattggtaca
    19141 gaaacattct gctgctcgcg ggagatagcg acatcttcaa catcgttatt ttccagcacg
    19201 ttcgatttct gcgtatcacc gtaaatcact ggaatatcgc attccgggca ggctactttc
    19261 ggtttcgctt cgaaaatcac gccgccctga tcgttttcaa ttttgctgat aaaccacggg
    19321 tccactagga agccgccgtt cgccatgacc gcgtagccgc gcgccacctg cattggggtg
    19381 aaggacgctg aacccagcgc cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg
    19441 aagcgttgca gatattctgc agcgtagtcg acgcccatcg cccgcattgc acgtaccatc
    19501 accacgtttt tcgactgacc cagcccctga cgtaagcgaa ttggaccagc atactgcggt
    19561 ggtgagttct tcggctgcca gtcagaaccg gcacctgcat cccagcgaga aattggcaca
    19621 tcgttcaaca tacttgccag cgtcagacct ttatccatcg ccgcggtgta gaggaacggt
    19681 ttgatgttgg aacccacctg acgcagtgcc tgggtggcgc ggttaaactt gctctgattg
    19741 aaatcaaagc caccgaccag cgccataacg gcaccatttt gcggattgat cgacaccagc
    19801 gccgagttca cttccggcac ttgtgccagc caccatgcat cgccaacctg acgaacccag
    19861 atttgctgac ccgtttgcag aacatcggtc actttacgcg gcgtcggtcc ctgctgagta
    19921 tccgaacggt aaggacgcgc ccagcgaacg ccttccatac tcaatgcgac ggtcgacccg
    19981 tccgccagca tcgccgtcgc tccctgagga ttggcgctgg tgactgcggc aggcagcagc
    20041 ggaccatagg tcggcagcgc cttcagcgta tcggtaatct tgttgttatc ccacgccgac
    20101 tcgcccactt tccacagcac atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc
    20161 agcacgttat tacgtaccgc ctgctgcgcg gcctgctgca ctttgcgggt gatggtggtg
    20221 taaatgcgat aaccgtcttc ataggcactt tcgccataac ggttatacat ctcctggcgc
    20281 accatttcgc tcaggtacgg cgcagagaaa gcaatctccg gcgcgtgata attagcgtta
    20341 atcgcctcag tgcgtgtctg atcgaactgt tgctgggtga tatacccttc atccagcatc
    20401 cgcgacagta cgacgttacg ccgcgcgacg gcacgatcca ttgagtagag cggattaaag
    20461 gtggaaggcg ctttcggcaa cccggctatc accgccattt cgttcagcgt cagttggtcg
    20521 accgttttac cgaaatagac ttgtgccgca gcaccgacac cataggcgcg gtaaccaagg
    20581 taaatcttgt tcagataaag ctcgaggatt tcgtctttcg tcagcaactg ttcaatgcga
    20641 atcgcgagga agacttcctt aatcttacgc atcatcgtgc gttctggact gaggaagaaa
    20701 tttctcgcca gctgctgggt aatggtactc gccccttgtg acgcgtggcc ggagaacagc
    20761 gccacacttg ccgcacggaa gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg
    20821 ctgtcttccg tcgcgataaa ggctttcacc atctccggtg ggatttgatc caacgtaacc
    20881 ggaatacgac gtttctcacc gtattgagca atcagctcgc catcggcgct gtaaatctgc
    20941 atcggaattt gcaggcgaac atcttttaat gtcaccacat ccggcagttg tggctcgatg
    21001 tagcggtata ggccataaat cgagcctgct cccagcagaa tgcaacagac tgcaaggatc
    21061 aaaaaatact ttacgaactt cactggaaat ttcccattta gtttcatttg ggcagtttat
    21121 aaacaaacgc gcggtagtat aaaggcaagc cagacgcatt gatatacccg tcagagtgac
    21181 gggtgataag gagatcatca caatgacatt taagatctgg caaattggtt tgcatttaca
    21241 acagcaagaa gcggcagcgg ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg
    21301 ctggtggcgg ttgccgctgg cgcacgacat tatcaaagat gggcggattg ttgatgcgca
    21361 gcagctggct aaaacgttgt taccgtggag tcgcgaactg ccgcagcgtc atcacattat
    21421 gttggcgttt cccgccagtc gcacattaca gcggtcattt ccgcgcccgt cgatgtccct
    21481 tggtgagcgg gagcaaacgg cctggctgtc agggacgatg gcccgcgagc tggatatgga
    21541 tccggactcc ctgcgcttcg attacagcga agactcactc agccccgctt ataacgtgac
    21601 tgccgcgcaa agcaaagagc tggcaacgct gcttacgctg gcagaaaggt tgcgtgttca
    21661 tgttagtgcg atcaccccgg atgccagtgc attacagcga ttcctgcctt ttttaccttc
    21721 tcatcagcaa tgtctggcct ggcgtgataa cgaacagtgg ctgtgggcga cgcgctatag
    21781 ctgggggcgc aaactggcgg tggggatgac tagcgcgaag gagctggcgg cagcgttatc
    21841 cgttgatccc gacagcgtcg cgatatgtgg cgaaggcgga tttgacccct gggaggccgt
    21901 ttctgttcgt cagccgccgc taccgccgcc tggtggagac tttgccatcg cgctggggct
    21961 ggcgcttggg aaggcgtact gatgaatccg ccaattaatt ttttgccctg gcgacagcaa
    22021 cgccggaccg cttttctgcg tttctggttg ctgatgttcg ttgcgcctct gctgctggcc
    22081 gtcgggataa cgctaatact gcgtctgaca ggcagcgccg aagcgcgcat agacgcagtt
    22141 ttgcttcagg cggaacaaca actcgcccgc agcttacaga taacgaagcc acgtttgctg
    22201 gagcaacagc agttacgcga acagcgttcg caacggcagc gccagcgaca atttacccgc
    22261 gactggcaat ctgcgctgga agcactggcg gcgcttttac ccgagcacgc ctggctgaca
    22321 acgataagct ggcagcaggg aacgctggag atcaaggggc tgacaacaag cattaccgcg
    22381 ttaaacgcac tagaaacgtc actccgccag gatgcgtctt ttcatctcaa tcagcgggga
    22441 gccacgcagc aggatgcgca gggacgctgg caatttgagt atcagttaac aaggaaggtt
    22501 agcgatgaac atgttctttg actggtggtt cgccacatca ccccgcctcc gccagttttg
    22561 ctgggcattc tggttgctga tgttagttac gctcattttt ctatcatcga cacaccatga
    22621 agagcgcgac gcattaattc gactacgggc aagtcatcac cagcagtggg ccgcactgta
    22681 tcgcctggta gacaccactc ccttcagcga ggaaaaaacg ctgccctttt cgccactgga
    22741 ttttcagtta tccggcgcgc aactggtttc ctggcatcca tccgcgcagg gaggtgagtt
    22801 ggcgttgaaa acgctgtggg aagcagtgcc gtcggcattt acacggctgg cagagcgcaa
    22861 cgtcagcgtg agccgttttt cgttaagcgt ggaaggtgat gatcttttgt tcacgctaca
    22921 actggagatg ccgcatgagg gttaaacgct ggttgttggc aggtattgca ttgtgccttt
    22981 taaccggtat gcgtgaccct tttaaatcgc cggaagatct atatgccgga ttagcgaact
    23041 tagccagtgg cgctatcagg ggatggtagg gcgaggcgag cgcatcatcg gtgtaataaa
    23101 agacgggcaa aagaaatggc gacgggtgca gcaaaacgat gtgctggaaa acggctggac
    23161 aattttacag ctgacgccag acgtactaac gctgggtacc gggacaaact gcgaaccgcc
    23221 acaatggttg tggcaacggc aaggagatac aaatgaagca atggatagcc gcactactgt
    23281 tgatgctgat acccggcgta caggcggcaa agccgcaaaa agtgacgctg atggtggatg
    23341 acgttccggt agctcaggtg ttgcaggcgc tggctgaaca ggagaagttg aacctggtcg
    23401 tgtcgccaga cgtcagcggt acggtgtcgt tacatctaac agatgttccc tggaagcagg
    23461 cactacaaac tgtagtgaaa agcgccggac tgataacgcg gcaggaaggc aacattctct
    23521 cagtgcattc cattgcctgg cagaatgaca atatcgcccg ccaggaggcg gagcaggcgc
    23581 gggcgcaggc aaatctgccg ctggaaaatc gcagtataac cctgcaatac gccgacgcgg
    23641 gagaactggc gaaagcgggg gagaagctac tgagtgccaa agggagtatg accgtcgata
    23701 aacgcaccaa tcgccttttg ctacgagata acaaaacggc gttaagcgcg cttgaacagt
    23761 gggtagcgca aatggatctg ccggtcgggc aggttgagct gtcggcgcat attgtcacca
    23821 ttaatgaaaa aagtttgcgt gagttaggcg tgaaatggac gctggccgat gcgcaacacg
    23881 ctggtggcgt tgggcaagtc accacgcttg gtagcgacct ctccgtagcg acggcgacaa
    23941 cgcatgtcgg ttttaacatt gggcgcatca acggacgctt gctggatctt gagctttccg
    24001 cgctcgaaca aaaacagcag ctggatatta tcgccagtcc gcgtctgctg gcctcacatc
    24061 ttcagcctgc cagcattaaa caggagagcg aaattccata tcaggtttcc agcggggaaa
    24121 gtggcgcgac gtcggtggaa tttaaagagg ccgtcctggg gatggaggtc acgcccacgg
    24181 tgttacaaaa aggtcgcatc cggctgaaat tacacatcag ccagaacgtt ccggggcagg
    24241 tgctacagca ggccgatggc gaagtgctgg cgattgataa gcaggagatc gaaacgcagg
    24301 tcgaggtaaa aagcggagaa acgttggcgc tgggcggcat ttttacccgt aaaaataaat
    24361 cgggtcagga tagcgtaccg ttgcttggcg acattccctg gttcgggcaa ttatttcgtc
    24421 atgacggaaa agaagatgaa cgacgcgagt tagtggtgtt tatcacgcca cgactggttt
    24481 ccagtgagta aacagccgta aaagcggtaa tgtttttatg ctgaacgtgt ttcatctatt
    24541 tgacgcgcgc aggtatttag catacaagga gtaccgattt gagagttggt gctcttcgct
    24601 gcctgcgttc catgatgatg atttatcatt caggcggcat tttgctgtct tttttacgct
    24661 aatcttaccc ggcggtgatt tatcgccaga gcggtggtag caaggcagcg cgcttgcagc
    24721 gaccagatat gcagagggat gggtgattta ttcagttgcc aaacccgctg gagtattgag
    24781 ataattttca gtctgactct cgcaatatct tatgaggttt cagttcatgt cctgcggcgc
    24841 tctctgagcg aagcgggttt atcattaacg aatagtctta gtagtaccga aaaaatggca
    24901 gagaaacgca atatctttct ggttgggcct atgggtgccg gaaaaagcac tattgggcgc
    24961 cagttagctc aacaactcaa tatggaattt tacgattccg atcaagagat tgagaaacga
    25021 accggagctg atgtgggctg ggttttcgat ttagaaggcg aagaaggctt ccgcgatcgc
    25081 gaagaaaagg tcatcaatga gttgaccgag aaacagggta ttgtgctggc tactggcggc
    25141 ggctctgtga aatcccgtga aacgcgtaac cgtctttccg ctcgtggcgt tgtcgtttat
    25201 cttgaaacga ccatcgaaaa gcaacttgca cgcacgcagc gtgataaaaa acgcccgttg
    25261 ctgcacgttg aaacaccgcc gcgtgaagtt ctggaagcgt tggccaatga acgcaatccg
    25321 ctgtatgaag agattgccga cgtgaccatt cgtactgatg atcaaagcgc taaagtggtt
    25381 gcaaaccaga ttattcacat gctggaaagc aactaattct ggctttatat acactcgtct
    25441 gcgggtacag taattaaggt ggatgtcgcg ttatggagag gattgtcgtt actctcgggg
    25501 aacgtagtta cccaattacc atcgcatctg gtttgtttaa tgaaccagct tcattcttac
    25561 cgctgaaatc gggcgagcag gtcatgttgg tcaccagcga aaccctggct cctctgtatc
    25621 tcgataaggt ccgcggcgta cttgaacagg cgggtgttaa cgtcgatagc gttatcctcc
    25681 ctgacggcga gcagtataaa agcctagctg tactcgatac cgtctttacg gcgttgttac
    25741 aaaagccgca tggtcgcgat actacgctgg tggcgcttgg cggcggcgta gtgggcgatc
    25801 tgaccggctt cgcggcggcg agttatcagc gcggtgttcg tttcattcaa gtcccgacga
    25861 cgttactgtc gcaggtcgat tcctccgttg gcggcaaaac tgcggtcaac catcccctcg
    25921 gtaaaaacat gattggcgcg ttctaccagc ctgcttcagt ggtggtggat ctcgactgtc
    25981 tgaaaacgct tcccccgcgt gagttagcgt cggggctggc agaagtcatc aaatacggca
    26041 ttattcttga cggtgcgttt tttaactggc tggaagagaa tctggatgcg ttgttgcgtc
    26101 tggacggtcc ggcaatggcg tactgtattc gccgttgttg tgaactgaag gcagaagttg
    26161 tcgccgccga cgagcgcgaa accgggttac gtgctttact gaatctggga cacacctttg
    26221 gtcatgccat tgaagctgaa atggggtatg gcaattggtt acatggtgaa gcggtcgctg
    26281 cgggtatggt gatggcggcg cggacgtcgg aacgtctcgg gcagtttagt tctgccgaaa
    26341 cgcagcgtat tataaccctg ctcacgcggg ctgggttacc ggtcaatggg ccgcgcgaaa
    26401 tgtccgcgca ggcgtattta ccgcatatgc tgcgtgacaa gaaagtcctt gcgggagaga
    26461 tgcgcttaat tcttccgttg gcaattggta agagtgaagt tcgcagcggc gtttcgcacg
    26521 agcttgttct taacgccatt gccgattgtc aatcagcgta acaacaagaa aggtcaggcc
    26581 gcttatcaag cggtctatta gcttcaggtt aattgcaacg tggtaagcat taacctttta
    26641 gtggggtgtt aaatggatga attcaaacca gaagacgagc tgaaacccga tcccagcgat
    26701 cgtcgtactg gtcgttctcg tcaatcttct gaacgttctg agcgtactga acgtggcgaa
    26761 ccgcagatca attttgatga tattgaactt gatgacactg acgatcgccg tccgactcgt
    26821 gcgcaaaaag agcgcaatga ggaaccggaa atcgaagaag aaattgacga atccgaagat
    26881 gaaaccgtgg atgaagagcg cgtagagcgt cgtccgcgta agcgcaaaaa agcagccagt
    26941 aaacccgctt ctcgtcagta tatgatgatg ggcgtcggca ttctggttct actgctgttg
    27001 atcatcggta tcggttctgc gctaaaagcc ccctcgacca cttccagcga tcaaaccgcg
    27061 tctggcgaga agagtattga tcttgctggc aatgcgaccg atcaggcgaa tggcgtgcag
    27121 ccagcgccgg gaaccacgtc tgcggaaaat actcagcagg atgtttctct gccaccgatc
    27181 tcttctacgc cgactcaagg gcaaaccccg gtggcaacgg atggtcaaca acgtgttgaa
    27241 gtgcagggtg acctgaacaa tgcgctgacc cagccacaaa atcagcaaca gctgaacaat
    27301 gtggcggtca attccacgtt gccgactgaa cccgcaacgg ttgcgcctgt tcgcaatggc
    27361 aatgcatcgc gtgacacggc gaaaacgcaa accgctgaac gtccgtccac tacgcgccca
    27421 gttcgtcagc aggcggtgat tgaaccgaaa aaaccgcaag caaccgtgaa aacggagccg
    27481 aagccggtag cacagacgcc gaagcgtact gaacctgaac cagctgcccc tgtggcgagc
    27541 acgaaggcac cggctgcgac ttctacgcca gcaccaaaag agacggcgac tacggctcca
    27601 gtacagacgg catccccggc gcaaaccacg gcaacaccag ccgctggagg gaagaccgca
    27661 ggtaatgttg gttcgttgaa atcggcaccg tccagccatt acactctgca gctgagcagt
    27721 tcctctaact acgacaacct gaacggttgg gcgaagaaag agaatctgaa aaactacgtt
    27781 gtctatgaaa cgacgcgtaa tggtcagccg tggtatgtcc tggtttctgg cgtgtacgct
    27841 tcgaaagaag aggcgaaaaa agcggtatct acattgccag cagatgttca ggccaaaaac
    27901 ccgtgggcga aaccgctgcg tcaggtacag gccgatctga agtaatcaag gttatctccc
    27961 gcaatggttt atcgttgcgg gagctgcctg aagcgctgga tgctgtcgga gctttctcca
    28021 cagccggaga aggtgtaatt agttaatcgg catgaagaaa aatcgcgctt ttttgaagtg
    28081 ggcagggggc aagtatcccc tgcttgatga tattaaacgg catttgccca agggcgaatg
    28141 tctggttgag ccttttgtag gtgccgggtc ggtgtttctc aacaccgact tttctcgtta
    28201 tatccttgcc gatatcaata gcgacctgat cagtctctat aacattgtga agatgcgtac
    28261 tgatgagtac gtacaggccg cacgcgagct gtttgttccc gaaacaaatt gcgccgaggt
    28321 ttactatcag ttccgcgaag agttcaacaa aagccaggat ccgttccgtc gggcggtact
    28381 gtttttatat ttgaaccgct acggttacaa cggcctgtgt cgttacaatc tgcgcggtga
    28441 gtttaacgtg ccgtttggcc gctacaaaaa accctatttc ccggaagcag agttgtatca
    28501 cttcgctgaa aaagcgcaga atgccttttt ctattgtgag tcttacgccg atagcatgga
    28561 gcgcgcagat gatgcatccg tcgtctattg cgatccgcct tatgcaccgc tgtctgcgac
    28621 cgccaacttt acggcgtatc acacaaacag ttttacgctt gaacaacaag cgcatctggc
    28681 ggagatcgcc gaaggtctgg ttgagcgcca tattccagtg ctgatctcca atcacgatac
    28741 gatgttaacg cgtgagtggt atcagcgcgc aaaattgcat gtcgtcaaag ttcgacgcag
    28801 tataagcagc aacggcggca cacgtaaaaa ggtggacgaa ctgctggctt tgtacaaacc
    28861 aggagtcgtt tcacccgcga aaaaataatt ctcaaggaga agcggatgaa acagtatttg
    28921 attgccccct caattctgtc ggctgatttt gcccgcctgg gtgaagatac cgcaaaagcc
    28981 ctggcagctg gcgctgatgt cgtgcatttt gacgtcatgg ataaccacta tgttcccaat
    29041 ctgacgattg ggccaatggt gctgaaatcc ttgcgtaact acggcattac cgcccctatc
    29101 gacgtacatc tgatggtgaa acccgtcgat cgcattgtgc ctgatttcgc tgccgctggt
    29161 gccagcatca ttacctttca tccagaagcc tccgagcatg ttgaccgcac gctgcaactg
    29221 attaaagaaa atggctgtaa agcgggtctg gtatttaacc cggcgacacc tctgagctat
    29281 ctggattacg tgatggataa gctggatgtg atcctgctga tgtccgtcaa ccctggtttc
    29341 ggcggtcagt ctttcattcc tcaaacactg gataaactgc gcgaagtacg tcgccgtatc
    29401 gacgagtctg gctttgacat tcgtctggaa gtggacggtg gcgtgaaggt gaacaacatt
    29461 ggcgaaatcg ctgcggcggg cgcggatatg ttcgtcgccg gttcggcaat cttcgaccag
    29521 ccagactata aaaaagtcat tgatgaaatg cgcagtgaac tggcaaaggt aagtcatgaa
    29581 taagtttgaa gatattcgcg gcgtcgcttt tgatcttgat ggtacgctgg tcgacagtgc
    29641 tcctggtctt gctgctgcgg tagatatggc gctgtatgcg ctggagttgc ccgtcgcagg
    29701 tgaagaacgc gttattacct ggattggtaa cggcgcagat gttctgatgg agcgcgcatt
    29761 gacctgggcg cgtcaggaac gtgcgactct gcgtaaaaca atggggaaac cgcccgttga
    29821 tgacgacatt ccggcagaag aacaggtacg tattctgcgt aaactgttcg atcgctacta
    29881 tggcgaggtt gccgaagagg ggacgttttt gttcccgcac gttgccgata cgttgggcgc
    29941 gttgcaggct aaaggcctgc cgctaggctt ggtcaccaac aaaccgacgc cgttcgtcgc
    30001 gccgctgctc gaagccttag atattgccaa atactttagc gtggttatcg gcggcgatga
    30061 tgtgcaaaac aaaaaaccgc atccggaccc gctgttactg gtggctgagc ggatgggaat
    30121 tgccccacaa cagatgctgt ttgtcggcga ctcacggaat gatattcagg cggcaaaagc
    30181 ggcaggttgc ccatcagttg gcttaaccta cggatacaac tacggcgagg ctatcgatct
    30241 cagccagcct gatgtaattt atcagtctat aaatgacctt ctgcccgcat tagggcttcc
    30301 gcatagcgaa aatcaggaat cgaaaaatga ctaagcccat cgtttttagt ggcgcacagc
    30361 cctcaggtga attgaccatt ggtaactaca tgggtgcgct gcgtcagtgg gtaaacatgc
    30421 aggatgacta ccattgcatt tactgtatcg ttgaccaaca cgcgatcacc gtgcgccagg
    30481 atgcacagaa gctgcgtaaa gcgacgttgg atacgctggc cttgtatctg gcttgtggta
    30541 tcgatcctga gaaaagcacc atttttgttc agtcccacgt accagaacat gcgcagttag
    30601 gctgggcact gaactgctat acctacttcg gcgaactgag tcgcatgacg cagtttaaag
    30661 ataaatctgc gcgttatgcc gagaacatca acgctggtct gtttggctat ccggtgctga
    30721 tggctgcgga catcctgctg tatcaaacta atctggtacc ggtgggtgaa gaccagaaac
    30781 agcacctgga actgagtcgc gatatcgccc agcgtttcaa cgcgctgtat ggcgagatct
    30841 ttaaggtgcc ggagccgttt attccgaaat ctggcgcgcg cgtaatgtcg ctgctggagc
    30901 cgaccaagaa gatgtccaag tctgacgata accgcaataa cgttatcggc ctgctggaag
    30961 atccgaaatc ggtagtgaag aaaatcaaac gcgcggtcac tgactccgac gagccgccgg
    31021 tagttcgcta cgatgtgcag aacaaagcgg gcgtttccaa cctgctggat atcctttctg
    31081 cggtaacggg ccagagcatc ccagaactgg aaaaacagtt cgaaggcaag atgtatggtc
    31141 atctgaaagg tgaagtggct gatgccgttt ccggtatgct gactgaattg caggaacgct
    31201 atcaccgttt ccgcaacgat gaagccttcc tgcaacaggt gatgaaagac ggcgcagaaa
    31261 aagccagtgt acacgcttcc cgtacgctga aagcggtgta cgaagcgatt ggttttgtgg
    31321 cgaagccgta atatcaaaac gctcggacgg tcccctcgcc cctttgggga gagggttagg
    31381 gtgaggggag caggcaagcg ccagtgggaa ttaccctttg ctcgcattgt cgaataaatc
    31441 acttcgtcat cactctgaaa ccggggaaac ccggtttttt tatcctcttt tcgcaacaac
    31501 ccgaaaaaat gtgaagcgcc tcgccgtttc cacatctcgt cgtcgccact tatcttcatt
    31561 ttaatgaaga tataaatatt cattttattg aaaatatatt atgcgtcgaa cgtttatcaa
    31621 aaaagaaggc gtcgtcatca cgacactggc ccgttatttg ttgggtgaaa aatgcggtaa
    31681 tcgactgaaa accatagatg agctggcaac tgaatgccgt tcatcggttg gcctgacgca
    31741 ggccgcgttg aaaacgctgg aatcaagcgg agcgatacgg attgaacgcc gtgggcgcaa
    31801 tggcagttat cttgtcgaga tggataacaa agcattgctg agtcatgtgg atatcaacaa
    31861 cgtggtatgt gcgatgcctt tgccctatac ccgcttgtac gaaggtttag cgagcggatt
    31921 gaaagcccag tttgatggca ttccttttta ctatgcgcat atgcgcggcg cggatattcg
    31981 cgtggagtgt ttacttaacg gtgtctatga catggcagta gtttcccgcc tggcggcgga
    32041 aagttatctc agccaaaaca atttgtgcat cgccctcgag cttggcccgc atacgtatgt
    32101 tggtgaacat caactgatat gccgcaaagg tgagtctgga aatgttaagc gagtaggtct
    32161 cgatagtcgt tcggcggatc agaaaatcat gaccgatgtc ttttttggcg atagtgatgt
    32221 ggaacgagtc gatctctctt atcacgagag tttacaacgc attgttaaag gcgatgtcga
    32281 tgcggtgatc tggaacgtgg tggcggaaaa cgaactgacc atgctgggat tagaggcgac
    32341 gccgctaaca gacgatccgc gatttttaca ggccaccgaa gccgtggtcc tgacgcgagt
    32401 cgatgattac ccaatgcaac aattgctgcg tgctgttgta gataaacacg ccctgcttgc
    32461 ccatcaacaa cgggtggtga gtggggaaca ggaacccagt tattaaacga aaggcatctg
    32521 atatggaaac cagactcaac ctgctttgcg aggcaggcgt tattgataag gacgtctgca
    32581 aaggcatgat gcaggtcgtc aacgtactgg aaaaagagtg ccatctgccg gtgcgcagtg
    32641 agcaaggaac gatggcgatg acacatatgg cgagtgcact gatgcgcagt cgccgtggtg
    32701 aagaaataga gccgctggat aacgagttgc tggcggaact ggcgcaatcc agccactggc
    32761 aagccgttgt gcaattgcat caggagttgt tgaaggaatt cgctctggaa gttaacccgt
    32821 gtgaagaagg ctattttctg gccaatcttt atggattatg gatggctgct aacgaagagg
    32881 tttgaagctc ccgacaaatc cacgtcgcac aacctaaata ttcaaaaaaa tgaatattta
    32941 attcaaggaa cagaaacgag gcaacaagat gtttgtagaa gcattgaaac gccagaaccc
    33001 ggcgctgatt tccgccgcac taagtttgtg gcagcagggc aaaatcgccc cggacagctg
    33061 ggtgatcgac gtggatcaga tactggaaaa cggtaagcgg ctcattgaga cggcgcggct
    33121 ttacggcatt gaactgtatc tgatgaccaa gcaatttggt cgtaatccgt ggctggcgga
    33181 aaaattgctg gcattaggct acagcggcat tgtggcggtg gattacaaag aggcgcgagt
    33241 catgcgccgc gctggtttgc cagtggcgca tcaggggcat ctggtacaaa tcccttgtca
    33301 tcaggttgct gacgccgttg aacagggcac cgacatcatc accgtgttta ctctcgacaa
    33361 agcgcgggaa gtttctgcgg cagcggttaa ggccggacgg gtccagcctg tgctgctcaa
    33421 agtttatagc gacgatgatt ttctttatcc ggggcaggag agcggttttg tacagcattc
    33481 gctgcacgag gtgattgctg aaattaagaa actgccgggg ctgcatttag ccggactaac
    33541 ccatttccct tgcctgcttt gggatgaggc tgctgggaaa gttttgccaa caccgaatct
    33601 tcacacgctg gtacaagcac gggatcaact ggcgaaatct ggtattgcga ttgagcaact
    33661 gaacgtacct tcagcgacca gctgcacttc gctgccattg ctggcggaat acggtgcgac
    33721 tcatgccgaa cccggtcatg cgctgaccgg tactattccg gcaaaccagc agggcgatca
    33781 gcctgaacgt atcgcgatgc tctggttaag tgaaatctcc catcatttcc gtggcgacag
    33841 ctactgctac ggcggcggtt actaccgtcg tggtcatgcg caacatgcgc tggtgtttac
    33901 gccagaaaat caaaagatta ctgaaaccaa tctcaaaact gtggatgaca gcagtatcga
    33961 ctacaccctg ccgctggcag gcgagtatcc ggtgagcagt gcagtggtgc tctgttttcg
    34021 cacgcagatt tttgtcaccc gtagcgatgt ggtgctggtg tccggtattc agcgcggcga
    34081 accagaaata gttggtcgtt atgacagcct cggcaaccct ctggaggcat aaatggcgcg
    34141 atttgtggtg ttagtgattg atagctttgg cgtaggggca atgaaagatg tcacgctggt
    34201 gcgcccgcaa gatgccgggg cgaatacctg tggtcacatc ctgagccagt tgccgcattt
    34261 gcagctacca acgctggaga agctggggct aatcaacaca ttgggttatg cgccaggcga
    34321 tatgcagccg tcagattccg caacctgggg cgtggcagag ctgcaacatg aaggtggcga
    34381 tacctttatg gggcatcagg aaattttagg cacgcgcccg ttaccgccgc tgcggatgcc
    34441 ttttcgcgat gtgattgacc gtgttgagca ggcattagtt tccgctggct ggcaggtgga
    34501 gcgccgtggc gatgatctgc aatttctgtc ggtcaatcag gcggttgcga ttggcgataa
    34561 tctcgaggcg gatttaggcc aagtctataa cattaccgcc aatctctctg tgatctcttt
    34621 tgacgacgca atcaaaattg gtcgtatcgt gcgtgaccag gtacaggtcg gtcgggtcat
    34681 tacatttggc ggcctgttaa ccaacagtca gcgtattctc gatgccgcag aaagcaaaga
    34741 agggcgcttt attggtatta atgcgccgcg ttctggcgct tatgacaacg gcttccaggt
    34801 cgtgcatatg ggctacggtg ttgatgaaaa agtgcaggtg ccgcaaaaac tgtatgaagc
    34861 gggcgtgcca accgtgctgg tgggtaaggt ggcagatatc gtcagcaatc cttatggtgt
    34921 gagctggcaa aacctggtgg atagccagcg gattatggat atcaccctcg acgaatttaa
    34981 cacccatccg acggcgttta tttgcatcaa tatccaggaa accgacctcg ctggtcatgc
    35041 agaagacgtc gcacgttatg ccgaacgttt gcaggtcgtt gaccgtaacc ttgcccggct
    35101 tgttgaggcg atgcagccag atgattgcct ggtcgtgatg gcggatcacg gtaacgatcc
    35161 gaccattggt cacagccacc atacccgtga agtggtgcca gtgctggttt atcagcaagg
    35221 gctggtcgcc acgcagctcg gtgtgcgcac cacgctttct gatgtgggag ctaccgtgtg
    35281 tgaatttttc cgtgcgccac cgccacaaaa tggtcgctct tttctttcct ccctccggtt
    35341 tgcaggagac accctatgag ttttgatccg acgggttaca ccctggccca tgagcatctg
    35401 catattgatc tctccggctt taaaaacaac gtggactgcc gccttgatca gtatgcgttc
    35461 atttgccagg agatgaacga cctgatgacc cggggtgtac gtaatgtgat tgagatgacc
    35521 aaccgttaca tggggcgcaa tgcgcaattt atgctcgatg taatgcgcga aaccgggatc
    35581 aacgtggtgg cctgtactgg ttattaccag gatgcgtttt tcccggaaca tgtggtgacc
    35641 cgcagcgtgc aggaactggc gcaggagatg gtcgatgaaa ttgaacaggg tatcgatggc
    35701 acggagctga aagccgggat catcgcggag atcggctcca gcgaaggaaa gattacgccg
    35761 ctggaagaga aggtatttat tgccgctgcg ctggcgcata accagaccgg acgcccgatc
    35821 tccacgcata cgtcgttcag caccatgggg ctggaacaac tggctctgtt gcaagcccac
    35881 ggagttgatc tttcgcgcgt caccgttggt cactgcgatc tgaaagacaa cctcgacaac
    35941 attttgaaga tgatcgatct cggcgcgtac gtgcagttcg acaccattgg taagaacagt
    36001 tactacccgg acgaaaagcg cattgcgatg ctccacgcgc tacgtgaccg tgggttgctg
    36061 aaccgcgtca tgctgtcgat ggatattacg cgccgctccc atttaaaagc caatggtggt
    36121 tatggctatg actatttatt gaccaccttt attccgcaat tgcgccagtc aggattcagt
    36181 caggccgatg tggatgtgat gttacgtgaa aatccctctc aatttttcca ataaggacag
    36241 actcatgaaa aagattggcg ttgcaggctt acagcgtgag cagattaaaa aaaccattga
    36301 agcgacggct cctggctgtt ttgaagtttt cattcacaac gacatggaag cggcaatgaa
    36361 ggtgaaatcc gggcaactag attattacat tggcgcgtgt aataccggtg cgggcgcggc
    36421 attgtcgatt gccatcgcgg tgattggcta taacaaaagt tgcaccattg ccaaaccagg
    36481 cattaaagcg aaagacgagc atatcgccaa aatgatcgct gaagggaaag tggcgtttgg
    36541 cctttccgtt gagcacgtcg aacacgcgat tccgatgttg attaaccatc tgaaataaaa
    36601 ggcacgacta tggatctgta tattcagatt atcgtggtgg cgtgcctgac gggtatgaca
    36661 tcgcttctgg agcatcgctc ggcggctgtt tttcatgacg gcatccaccc gatcctgccg
    36721 caactgattg aaggctatat gaaccgtcgc gaggcgggga gtatcgcttt tggtctgagc
    36781 attggttttg tggcctcggt ggggatctct tttaccctga aaaccgggct gctcaacgca
    36841 tggttactct ttcttcctac cgatatcctc ggcgtactgg cgataaacag cctgatggcg
    36901 tttggtcttg gcgctatctg gggcgtgttg atccttactt gcctgttgcc agttaaccag
    36961 ctgctgaccg cgctgccggt ggatgtatta ggtagcctcg gggaattaag ctcgccggtg
    37021 gtttctgctt ttgcactgtt cccgctggtg gcgattttct accagtttgg ctggaagcaa
    37081 agtctggtcg ccgccgtggt tgttctgatg acccgtgtgg tagtcgtgcg ctatttccca
    37141 catcttaacc ctgaatccat cgaaatcttt attggcatgg tgatgctgct ggggatcgcg
    37201 ataactcacg acctgcgtca tcgtgatgaa aatgacatcg atgccagcgg gctttcggtg
    37261 tttgaagagc gcacgtcgcg gattatcaaa aacttacctt atatcgccat cgtgggagca
    37321 ttgattgccg ccgttgccag tatgaagatt tttgctggta gcgaagtttc gatcttcaca
    37381 ctggagaaag cgtattccgc aggcgtgacg ccggaacaat cgcaaacgct gattaatcag
    37441 gcggctctgg cagaatttat gcgcggactg gggtttgtgc cgttgattgc caccaccgcc
    37501 ttagcaacgg gtgtgtatgc agttgcgggc tttacctttg tttatgcggt gggctatctc
    37561 tcgccgaatc cgatggttgc agcggtatta ggcgcagtgg ttatttcggc ggaagtcttg
    37621 ctgcttcgtt cgatcggcaa atggctggga cgctacccgt cggtgcgtaa tgcgtcggat
    37681 aacatccgta acgccatgaa tatgctgatg gaagtggcgc tgctggtcgg ttcgattttc
    37741 gcagcaatta agatggcggg ttataccgga ttctctatcg cggttgccat ttacttcctc
    37801 aacgaatccc tgggccgtcc ggtacagaaa atggcggcac cggtcgtggc agtaatgatc
    37861 accggtattc tgctgaatgt tctttactgg cttggcctgt tcgttccggc ttaaggaagg
    37921 cacctatgaa gacgtttcct ttgcaaagcc tgactcttgc tgaagcgcag caaaagcagt
    37981 ttgcgctggt ggacactatt tgccgccatt ttccaggcag cgagtttctt gctggcggtg
    38041 att
//
LOCUS       DAJTYH010000029        37194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTYH010000029 DAJTYH010000000
VERSION     DAJTYH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..176)
                     /locus_tag="OO609_001455"
     CDS             complement(<1..176)
                     /locus_tag="OO609_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6632929.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO609_001456"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO609_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6632930.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO609_001457"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO609_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6632931.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO609_001458"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO609_001458"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6632932.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO609_001459"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO609_001459"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6632933.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO609_001460"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO609_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6632934.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="OO609_001461"
     CDS             complement(5180..5422)
                     /locus_tag="OO609_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6632935.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO609_001462"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO609_001462"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6632936.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO609_001463"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO609_001463"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6632937.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="OO609_001464"
     CDS             complement(8339..9313)
                     /locus_tag="OO609_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6632938.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="OO609_001465"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="OO609_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6632939.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="OO609_001466"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="OO609_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6632940.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="OO609_001467"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="OO609_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6632941.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="OO609_001468"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="OO609_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6632942.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="OO609_001469"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="OO609_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6632943.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="OO609_001470"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="OO609_001470"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6632944.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="OO609_001471"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="OO609_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6632945.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="OO609_001472"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="OO609_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6632946.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO609_001473"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO609_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6632947.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="OO609_001474"
     CDS             23180..23383
                     /locus_tag="OO609_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6632948.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="OO609_001475"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="OO609_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6632949.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="OO609_001476"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="OO609_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6632950.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="OO609_001477"
     CDS             26388..26660
                     /locus_tag="OO609_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6632951.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO609_001478"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO609_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6632952.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO609_001479"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO609_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6632953.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="OO609_001480"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="OO609_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6632954.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO609_001481"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO609_001481"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6632955.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO609_001482"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO609_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="OO609_001483"
     CDS             complement(31907..32305)
                     /locus_tag="OO609_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6632956.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="OO609_001484"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="OO609_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6632957.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="OO609_001485"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="OO609_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6632958.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="OO609_001486"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="OO609_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6632959.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="OO609_001487"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="OO609_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6632960.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="OO609_001488"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="OO609_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6632961.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..>37194)
                     /gene="dcuC"
                     /locus_tag="OO609_001489"
     CDS             complement(36260..>37194)
                     /gene="dcuC"
                     /locus_tag="OO609_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6632962.1"
                     /translation="FPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVAGMKI
                     ATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAIL
                     PVMPLILMLGSLFLAHVGLMQSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAF
                     FDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAI
                     CAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVVTS
                     GIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8994 a         9295 c         9796 g         9109 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatgac ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgactgcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aata
//
LOCUS       DAJTYH010000030        36062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTYH010000030 DAJTYH010000000
VERSION     DAJTYH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..876)
                     /locus_tag="OO609_001490"
     CDS             complement(<1..876)
                     /locus_tag="OO609_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6632963.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNG"
     gene            complement(1023..1099)
                     /locus_tag="OO609_001491"
     tRNA            complement(1023..1099)
                     /locus_tag="OO609_001491"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1063..1065),aa:Pro,seq:tgg)
     gene            complement(1142..1228)
                     /locus_tag="OO609_001492"
     tRNA            complement(1142..1228)
                     /locus_tag="OO609_001492"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1192..1194),aa:Leu,seq:cag)
     gene            complement(1249..1324)
                     /locus_tag="OO609_001493"
     tRNA            complement(1249..1324)
                     /locus_tag="OO609_001493"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1289..1291),aa:His,seq:gtg)
     gene            complement(1383..1459)
                     /locus_tag="OO609_001494"
     tRNA            complement(1383..1459)
                     /locus_tag="OO609_001494"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Arg,seq:ccg)
     gene            complement(1562..2947)
                     /locus_tag="OO609_001495"
     CDS             complement(1562..2947)
                     /locus_tag="OO609_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6632964.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3138..3878)
                     /gene="rffM"
                     /locus_tag="OO609_001496"
     CDS             complement(3138..3878)
                     /gene="rffM"
                     /locus_tag="OO609_001496"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6632965.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(3881..5233)
                     /gene="wzyE"
                     /locus_tag="OO609_001497"
     CDS             complement(3881..5233)
                     /gene="wzyE"
                     /locus_tag="OO609_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6632966.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5230..6309)
                     /gene="rffT"
                     /locus_tag="OO609_001498"
                     /gene_synonym="wecF"
     CDS             complement(5230..6309)
                     /gene="rffT"
                     /locus_tag="OO609_001498"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6632967.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6306..7556)
                     /gene="wzxE"
                     /locus_tag="OO609_001499"
     CDS             complement(6306..7556)
                     /gene="wzxE"
                     /locus_tag="OO609_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6632968.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7558..8688)
                     /gene="rffA"
                     /locus_tag="OO609_001500"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7558..8688)
                     /gene="rffA"
                     /locus_tag="OO609_001500"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6632969.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(8693..9367)
                     /gene="rffC"
                     /locus_tag="OO609_001501"
                     /gene_synonym="wecD"
     CDS             complement(8693..9367)
                     /gene="rffC"
                     /locus_tag="OO609_001501"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6632970.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9345..10226)
                     /gene="rfbA"
                     /locus_tag="OO609_001502"
     CDS             complement(9345..10226)
                     /gene="rfbA"
                     /locus_tag="OO609_001502"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6632971.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10245..11312)
                     /gene="rffG"
                     /locus_tag="OO609_001503"
     CDS             complement(10245..11312)
                     /gene="rffG"
                     /locus_tag="OO609_001503"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6632972.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11312..12574)
                     /gene="wecC"
                     /locus_tag="OO609_001504"
     CDS             complement(11312..12574)
                     /gene="wecC"
                     /locus_tag="OO609_001504"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6632973.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12571..13701)
                     /gene="wecB"
                     /locus_tag="OO609_001505"
     CDS             complement(12571..13701)
                     /gene="wecB"
                     /locus_tag="OO609_001505"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6632974.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(13757..14803)
                     /gene="wzzE"
                     /locus_tag="OO609_001506"
     CDS             complement(13757..14803)
                     /gene="wzzE"
                     /locus_tag="OO609_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6632975.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(14815..15918)
                     /gene="wecA"
                     /locus_tag="OO609_001507"
     CDS             complement(14815..15918)
                     /gene="wecA"
                     /locus_tag="OO609_001507"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6632976.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16158..17417)
                     /gene="rho"
                     /locus_tag="OO609_001508"
     CDS             complement(16158..17417)
                     /gene="rho"
                     /locus_tag="OO609_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6632977.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17502..17603)
                     /gene="rhoL"
                     /locus_tag="OO609_001509"
     CDS             complement(17502..17603)
                     /gene="rhoL"
                     /locus_tag="OO609_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6632978.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(17744..18073)
                     /gene="trxA"
                     /locus_tag="OO609_001510"
     CDS             complement(17744..18073)
                     /gene="trxA"
                     /locus_tag="OO609_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6632979.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18204..19469
                     /gene="rhlB"
                     /locus_tag="OO609_001511"
     CDS             18204..19469
                     /gene="rhlB"
                     /locus_tag="OO609_001511"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6632980.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19603..21087
                     /gene="gppA"
                     /locus_tag="OO609_001512"
     CDS             19603..21087
                     /gene="gppA"
                     /locus_tag="OO609_001512"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6632981.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21134..23155)
                     /gene="rep"
                     /locus_tag="OO609_001513"
     CDS             complement(21134..23155)
                     /gene="rep"
                     /locus_tag="OO609_001513"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6632982.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23242..23523
                     /gene="ppiC"
                     /locus_tag="OO609_001514"
     CDS             23242..23523
                     /gene="ppiC"
                     /locus_tag="OO609_001514"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6632983.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23569..25044)
                     /gene="ilvC"
                     /locus_tag="OO609_001515"
     CDS             complement(23569..25044)
                     /gene="ilvC"
                     /locus_tag="OO609_001515"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6632984.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25194..26084
                     /gene="ilvY"
                     /locus_tag="OO609_001516"
     CDS             25194..26084
                     /gene="ilvY"
                     /locus_tag="OO609_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6632985.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26081..27625)
                     /gene="ilvA"
                     /locus_tag="OO609_001517"
     CDS             complement(26081..27625)
                     /gene="ilvA"
                     /locus_tag="OO609_001517"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6632986.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27628..29478)
                     /gene="ilvD"
                     /locus_tag="OO609_001518"
     CDS             complement(27628..29478)
                     /gene="ilvD"
                     /locus_tag="OO609_001518"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6632987.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(29543..30472)
                     /gene="ilvE"
                     /locus_tag="OO609_001519"
     CDS             complement(29543..30472)
                     /gene="ilvE"
                     /locus_tag="OO609_001519"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6632988.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30492..30755)
                     /gene="ilvM"
                     /locus_tag="OO609_001520"
     CDS             complement(30492..30755)
                     /gene="ilvM"
                     /locus_tag="OO609_001520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6632989.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30752..32398)
                     /locus_tag="OO609_001521"
     CDS             complement(30752..32398)
                     /locus_tag="OO609_001521"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6632990.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32401..32451)
                     /locus_tag="OO609_001522"
     CDS             complement(32401..32451)
                     /locus_tag="OO609_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632991.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32537..32635)
                     /gene="ilvL"
                     /locus_tag="OO609_001523"
     CDS             complement(32537..32635)
                     /gene="ilvL"
                     /locus_tag="OO609_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6632992.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            32987..34466
                     /locus_tag="OO609_001524"
                     /pseudo
     CDS             32987..34466
                     /locus_tag="OO609_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(34491..34829)
                     /gene="maoP"
                     /locus_tag="OO609_001525"
     CDS             complement(34491..34829)
                     /gene="maoP"
                     /locus_tag="OO609_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6632993.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34948..35787
                     /gene="hdfR"
                     /locus_tag="OO609_001526"
     CDS             34948..35787
                     /gene="hdfR"
                     /locus_tag="OO609_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6632994.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(35883..35958)
                     /locus_tag="OO609_001527"
     tRNA            complement(35883..35958)
                     /locus_tag="OO609_001527"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35923..35925),aa:Trp,seq:cca)
     gene            complement(35967..36043)
                     /locus_tag="OO609_001528"
     tRNA            complement(35967..36043)
                     /locus_tag="OO609_001528"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36007..36009),aa:Asp,seq:gtc)
BASE COUNT         8807 a         9868 c         9097 g         8290 t
ORIGIN      
        1 gccgttgtgc tcaacaatca ccgagcgccc acaggttggc tgatgatggc aaaccgcacc
       61 cggcgcaccg acaaaattgg caaacgccca ttcgatattc atcacgaaaa tcttgccgac
      121 gtcgcgtttg atccagtggt caaaaatcgc caccagaaat tcaccaaatt catcggggcg
      181 cacagaccat tccgtcagtt ccccctgaat atcaccaggc gcatgtagtt tcagtccatc
      241 gcggacagct gtttcatcag ccaggcgctc gaccaccgga ataaactgga tgaattcgac
      301 tcccgcatcg cacaaaaaat cataaacctg taacggttgc tgcgcgctgg tgcggttgac
      361 gcagaccagc acgttatagt cgacatgatg tttttgcagg agcgtcaggg cacgcatcac
      421 cagtttatgc gtgggtctgc cgcctttggt cacgcgatat tgattgtgga tctcagccgg
      481 accatccagc gataacccaa caagaaaatt attttctgcc agaaatgcac accattcgtc
      541 atcgagcagc acgccgttag tctggaaact gttacttatc ttcctgccag caccatattt
      601 cgcctgtagc gccacggcac ggcggtaaaa ctccagcccg agtagcgttg gttcgccgcc
      661 ctgccaggta aaagccactt cgttttgcag ttcgctggcg gcgatatagt gacggatata
      721 cgcttccagc gtgtcatcgt ccatatgcgt gactggcttt tcgcggtaaa gggattgttt
      781 ttcgagataa aaacagtagt cacagttcag attacaatcg gaaccgctcg gtttcgctat
      841 cacatgaaaa gcacgcgttg gaacctgttg cagcatggtc gctccttgcc tgaagtcatc
      901 cttcaatacg ttaagggcga aatcataccg tggggcaatg agttattacg ggcgtttatt
      961 gcgaagggag ggtgacgaaa tgcacagaaa acaaaaaacc ccgccgaagc ggggttcaaa
     1021 attggtcggc gagagaggat tcgaacctcc gacccactgg tcccaaacca gttgcgctac
     1081 caagctgcgc tactcgccga aatactgctt tttgaatttt tagttcaatt ctttaaagtc
     1141 gtggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta
     1201 gcgcgtctac caattccgcc accttcgcat tgtcacaact tctaataatg gggtggctaa
     1261 tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac tgagctatag
     1321 ccaccactac aaatcttgtt acgcggtatt actaccaccg cagctcaagc gctgggacta
     1381 aatggcgcgc ccgacaggat tcgaacctga gacctctgcc tccggagggc agcgctctat
     1441 ccagctgagc tacgggcgct tagcgccgtt gcgggggtgg ataatacgga cttcccaccc
     1501 ctctgtctag tcccttttta aataaattgc tcgtttggtt acggtttgtg cgttttgctg
     1561 cttattcctc cagtttatgc gctttaccgt ggcgattaag cccaaaaact ttataaatcg
     1621 ccgtcaccgc cagcataaag atgatgccga caaacagcga catacgcgta tcttcattaa
     1681 agtacatgcc gatcaaaacg caaatcagga atgccattgt tacgtagttg gcccacggga
     1741 acaggattga gcggaacgga tggctggcaa tcgccgcttt atgtgcacgc cgaaaacgca
     1801 gctggcttat caatatcaca aaccatggca ccatccccgg aagcacgctg gcactgtaga
     1861 cgtagacaaa cacacgctgc ggattgggaa tgatgtagtt caggcatgag ccaattagca
     1921 gaatagcaat agatactgcc acacccgcaa ccggtacgcc gtgacgggaa acttttgcca
     1981 ttgccgccgg taactgacgg tttttcgcca gtgcgtagag catacgtccg caactgtaca
     2041 tgccgctgtt acagccagag agcgcagccg tcagcaccac aaagttgata atgcccgccg
     2101 ctgcggtaat accgattttg gcaaaagtca gtacgaacgg gctgccgttg ctgccaattt
     2161 cattccacgg gaagatggtg acgataacga aaatcgcgcc tacgtagaaa atcaggatcc
     2221 gccacagcac tttgcctacg gcactgcgca gcgtcacctg cggattcttc gcttcaccgg
     2281 cagtaatgcc aatcagctcc acgccctggt aggacgccac cacaatacac agagcggtca
     2341 ggaacccttt ccagccaccc gcaaagaaac cgccatgctc tgtgagattg ctaaaaccaa
     2401 tcgactgccc gccattgcca aagccaaaga aaatcacgcc caggccaatg acaatcatca
     2461 cgataatcgt ggtgactttg atcatcgcga accagaactc gatttcgccg tacaaccgta
     2521 ccgccgccag attcgccaac gccaccagcg ccaccgcgat caatgcgggt atccactgcg
     2581 ccatctccgg ggaccaaaac tggacataaa cgccaatggc ggtgatttct gagatcccca
     2641 ccgccatcca cataaaccag taagaccagg cggtgagata gccaaaaaac gggctcatat
     2701 aacgatgcgc ataaacggcg aacgaaccgg taaccggttc gaggaacaac atttcgccca
     2761 ttgaacgcat gatgaaaaaa acgaacagcc cggcgatgat ataggccaac aatacggatg
     2821 gcccggccca tttcagggta ctggcggccc ccataaacag gccgacgcca atggtgcccc
     2881 ccagggcgat gagttcgata tgtcgagctt ccagcccacg ctgtagctct ggtttgttat
     2941 ctgccataaa tcctcgtgtt gtgtttgcat gctttccggt gttaccggtt atcgttatgg
     3001 gtacatcgag tgttgcgaat gttttcgtaa ttcaggagaa atggcaaata aagcattaaa
     3061 aatttgaatg ctttgtgtaa taaaaaagca gacaggcgac gaagtgacca ctccgtcgct
     3121 ttacaaagag aggaaaatca gaggttgccg gtgtagtgcc agcgtaaata acgcagcaaa
     3181 cgaagctgac gcttaatgcg gctcggctgc gaaagcaggc ggtagagcca ctccagtccc
     3241 agcgtttgcc agattttcgg tgcgcgtttt acgtgaccgg tgaaaacatc gtaagtcccg
     3301 ccaacgccca tatacagcgc atctggatgc accagacggc agtcgcgcat gaagatctcc
     3361 tgctttggcg atcccatcgc aacggtgacg atttgcgctc cgctggcatg aatgcgttca
     3421 aacagcgcct gacgctgctc gggtttaaaa taaccatcct gactgccaac gatattcaca
     3481 ttccattggt tgcgcagttt agcttcagtt tgcgccagca cttcaggttt accgcccaca
     3541 agaaataccg gcgtcccttc tttgcctgcg cgcgccatca gctcttccca gagatcggca
     3601 ccggcaacgc gggaaacctg cgcctgcggg tactttttac gtactgaacg tacaatgctg
     3661 atgccatccg catatttaaa ttcggcagcg ttaattaact ccctgacctc ggcgttatct
     3721 tcaatggtca gcattttttc ggcattaatg gcaaccagcg ttccctgctt aagctgcccg
     3781 tcagcaaaca gataatcgag ggcatgctgc atatcacgcc aaccaatcaa ctgtaagcca
     3841 cgcagcgtat aggtcggtgc cgtggtgttg ttattcattg ttatccttca acctgcgtcc
     3901 ggagcgatga ttttgtacgt ttatgaatga gtccggcgct ttcaaaaagc cagtacaaca
     3961 gttttgcgat catcagacat gcgccgaaga ccacgataaa aaagactacg cgtgagacaa
     4021 acgaatccag cccttcacgt gccagcacga tcatattgaa gatcgcccca aagcagaaac
     4081 tgtgcaatat cgcagcctta tagcgattag tctcgcggtt gcccagctca tacagccagt
     4141 cgaaccattt gatgatcagt ccaaccacaa tcgccccgag tgggatgaac aacgcgccgc
     4201 ccatcaccac cagtgagcct ataagcgtag gcgagatcgc cagtccggag tggttattca
     4261 gtacttccca ggtaaagtaa ttggctgagt tcagcaccat actcgggcgc cccggccaca
     4321 gccaggaagg gataaagaca tagaaatcgc ggacaattgg agccaggccc tggaagtcga
     4381 tgttgtcgta gttctgcaac agcaacgcca gattctccca cggtgaaaag gtgtcgcgag
     4441 tgagatagag aaacgtatag aacgcttcat cgccgctcac attcattcca tagcgtttta
     4501 acgccagcca gaacatgcca acaatcccca gcacgcccgc cgccgccagc atccacaacg
     4561 aaatccagcc gcgaataatg ccaataaaca ggaagatagc gaatgcgatg atgatattgg
     4621 cgcgagtgcc gccgacaatc atataagtca gcaagccaaa ggcgaccgtg ctgacgagga
     4681 aaaacagcca cgctttgctg tcctggcgca gaaagtagac caccagcatc gccgggatga
     4741 aaaagtaaaa gaagcgtttt aacgccacgc cggagacttc actggaaaag atctgactgt
     4801 aggagttaag ccggaacagc aaaaagccgt tgtgcatgaa gaagatgccg acgcttacca
     4861 gcgcgatacc catcaggatc acccacgtaa gattggtctc cacgcggttc atggtaaaca
     4921 gcggacggcg cggtacatca gcaacgcgtt tgcgtaggcg ggttttgtag gtgacatagt
     4981 aaaccgcgta gaagcagccc gcagaaagca acacctgcaa caagatttct ggcggcgcga
     5041 caccaacatc aaagcgaaat accagcacgc tggtcagcgg gaagccgaag aaaaaggtga
     5101 gcaaaaacag caatgaaaag aagacattga agttaaagcg cacacggcga aactcaaacc
     5161 aggtcagcgt ggcaataaac agcgtgcaga gcagccagac cacaaacagg ccactgaatt
     5221 gcagcagact catgcgacct ccccggcggc aatcgccaac gcccgctgcc agccttgtag
     5281 atagttaggg ctaaagaagg caatggtgtt tttatccgcc gacgccaact ggcgctgcgc
     5341 ttcacgcaca atatcctcgt tgagatcgtc ggtagtaaac agcactggca aatgctgttc
     5401 cgtcatatcc tgccagaacg gattttccct gttaagcaca caaggaatgc ccgcctgaat
     5461 cagtaagcaa agcgtaccaa tgccctgctg gcgggcaaaa ataaagtaac caagatcgca
     5521 ctgacgaagt agcgccagat aggcgtcaaa ttccagtttt tcgctcaaaa tttgcagatt
     5581 ttcttcgctg aataactcca gccccgcctg acgaacttcc tcaatgtacg cttcgttatt
     5641 aggcggatat cccatcggca ccaccacttt taccgtatcg ccaaattgct gatgaacggc
     5701 acgtaaagcg gcgacatgct cattgctgcg gtcgccggag ttccccacca gaatggtcat
     5761 ttttccttca cgttgccgat cgttcgccat cgtattgaga gaagggtcca tccgcgtcgg
     5821 gaagtacagc agttctcccc gcacctttgg gtgtgttttg gcaaaaaagc tcaaatcgcc
     5881 gcgggtggca aatacacagc cgactcgctt ttgcgccagg cgacgtagtg ggtaaaaaag
     5941 cttatatctc aagccactgg aaagctcata caggtctgcc ccccagatat gccagaaaaa
     6001 ctggctgggc ttaatgccac cactcagcag agccagccac agtgtgggat tgaactgacc
     6061 gtggaaaaaa aatcgctgct gacggttagc ttttgctttc gcgatgaccg cttccgccag
     6121 cgattttttc ccagggaaaa attgcacaga aagcgccgga cagctatcac ttaagccgtc
     6181 gtccttgcca acaaccataa actcgcgcgc gtgctcgctc gtcgcggcca gcgcgtcatt
     6241 gaaaaaccgc aaaacggttc ggttatggtg agggatatcc gatcccagta cgtgaatcag
     6301 tacagtcatg cccgcctacg ccagagtaaa aacacgccac aacaaagaga aaaatagacg
     6361 atataagttg ccatatatgc ctgcgccgcg cccagtgcac catgcgcagg gattagccag
     6421 tgggcaaata ccatcaataa agtgaactgg ctgacttccg ccagaatata aaaccgcagt
     6481 gacgctttgg cgatcaccag ataaccaaag acataagcgc ccacttttaa cacatcaccc
     6541 actaactgcc aggcaaagag atcgcgcata gcggtaaatt tattcgacaa cagcaaccag
     6601 atagcaaaat cacgcagcaa ccagacggta aaactcgctg ccgccactgc cggtaagacg
     6661 aatttcagcg atttaaccac ttcccgggtg atatcgcgct tttccgttag ccgcgacaac
     6721 gtgggcagca aatatacgct gaacgatgcc gtaataaatt gcaggtaggc atcggaaata
     6781 ctgctcaccc cttgccagat ccccacttca tcccagctat actgcgccgc cagcagttta
     6841 cgcatcatga tgtaagcaac aggcaaggtc accgacgtaa tcaacgccat gagcgtaaat
     6901 ttgctcaact gccctgccag accgttatcc cagctgggtt tcagatagct taacgggatg
     6961 acaccacgtt taatcaacat gatggcggca ggaattacca ccagcgcggg aatcagcgcc
     7021 agacccagca acgccccttc ataaccgccc aaacggtaac tgacgtagta cgcgagaacg
     7081 ccaatcaagc tgccgacaat cagcgataac gcattacctg cggcatcgcg aaagcctttc
     7141 atcagcgcca gtaacaggtt gccccaggcg atccccattt gcaccagcgc cactaaacgc
     7201 accagcccct gatagtcggt attaccaaac aatccctggc tgattggcgc agctgccagc
     7261 acaaaaacca gcgccatcag cgtagagaaa ccaagtacca tcgctgatga agtgccgacc
     7321 acgcggcgca gctgttgcgg attatcatgg tactgggcaa cgtatttggt tacaccgtta
     7381 aagatgccag ccccggcaag cacgccgagc acggtaatca actggcggaa atttgccgcc
     7441 agcccaagcc ccgccggacc aaatgacacc gccagcaact taccgaccag taacccggca
     7501 ccaatcttga ccagtgtact ggccgccgtc cacaaggacg cttttgccaa cgacatatca
     7561 ggagaagtag ttcaacaaag tcgcaattac cgtacgctga ttgacgggcg acaggttgta
     7621 gaacagcggc aggcgcagca ggcgctcgct ctctttggtg gtgtagcgat cttcaccgtg
     7681 gaactcacca aagtgttccc ccgcagggca accgtgcagc ggaatgtaat gaaacaccgc
     7741 catgatttcc gcttctttca gaaagttaat caacgcgctc cggtcatcaa tatcccgcag
     7801 tttaatgtag aacatatgcg cgttctgcac gcagccatcg ggaatcgacg gcagctcgat
     7861 acgcccggct ttcgccagag gcgctaacgt atcgtagtag ttttgccaca gcgccagacg
     7921 ttgctggttg atacgatccg ctgcttccag ttgcgcccac aggtacgcag cttgcagatc
     7981 ggacatcaaa tagctggagc caatatcgcg ccaggtatat ttatcgacct gaccacggaa
     8041 gaactggctg cggttagtgc ccttttcacg gatgatctcg gctcgttcga ttaacgcttt
     8101 atcgttaatc agcgtcgcgc cgccttcacc acccgccgtg tagtttttgg tttcatggaa
     8161 gctaaagcag ccaatatgac caatggttcc cagtgcacgc cctttgtaag tggacatcac
     8221 gccctgagcg gcatcttcca ccacaaacaa attatgcttt ttcgccaacg ccataatggt
     8281 gtccatttcg caggccacac ccgcgtaatg gaccggcacg ataacgcgcg ttttgtcggt
     8341 gatcgctgct tcaatcaacg tttcgtcgat gttcatggtg tccgggcgaa catccacaaa
     8401 gacgattttc gcgccacgca gcacaaaggc attggcggtg gagacaaagg tgtagctcgg
     8461 catgatcact tcatcgccag gctggatatc gagcagcagc gccgccatct ccagcgaagc
     8521 ggtgcaggac ggcgtcagta acactttggc gctgccaaaa cgttgctcca gccactgctg
     8581 gcagcgacgg gtaaaaccgc catcgccaca cagtttgccg ctacccattg ccgactgcat
     8641 atagtcgagt tcggttccca ccaccggcgg tgcgttaaat ggaatcatgt gatcacctgt
     8701 ataaccagta cgcggtgctt tctacattcg caccactttg tatgtatcgt ttaagcgcgg
     8761 cggtgttgcc catttgggtc gccacccgca aagttgtttt accgcgagca tacgcccagt
     8821 ttagcgccgt ttgcatcagc tcagcacctg caccgcgtcc agccagcagg ccaattcgcg
     8881 catctgtcgc attgagttcc cgtaaagaga catagccgcg aatatcgccg gacgccgcac
     8941 gtagaatcag acattgatga tcaaaggtgc cgcgcacggc attttcaatc cactgtgcat
     9001 aaaagcgacc gctggcgtca ggcgcatacc acggcgcacg aaaacggctt tgcgcaaatg
     9061 cggcgctagc taactgacgt aatgcgggaa tatcggtctc ttgtgccacg acagcaccgc
     9121 tatcactgac attgttcacg ggtagcgcca aatcaacttc accttctacc agggagaatc
     9181 ccagctgttg cagggcatcc agttcccccg tatttgatgc cgcaattttg gcctgcaccc
     9241 gtgaccatgg cgctaaggcg tctgacgtca ggagcggtgc ttcagacgta atgcgcacga
     9301 tggcgctgtt aacaccaaag aaggcgtttt cccaggttag tggctcaata ctggcgcgga
     9361 cgggcacgaa gtaactccag cagatattgg ccgtagccag ttttcgctaa tgaactggca
     9421 gcacgcttca caccctcgtc atcgagccag ccgttacgcc aggcgatctc ttccaggcag
     9481 gcaatcttaa agccctggcg tttttctact gtctgcacaa aagtgctggc ctcaatcagg
     9541 ctgtcgtgag tgccggtatc cagccaggca aatccgcgcc cgagcagttc aacggtcagg
     9601 ttgcccgcct cgaggtacat ctggttgatg gaggtaatct ccagttcacc gcgctccgac
     9661 ggcttcacct gctttgcgta ctccacgact ttactgtcgt agaaataaag cccggtcacc
     9721 gcccagtttg acttcggctg ttttggcttt tcttcaagcg agatagcgcg gaaattgtcg
     9781 tcaaattcca ccacgccaaa gcgttccggg tccatcacct gatagccaaa aaccgtcgcc
     9841 ccttccgtgc gcgccgcaac atgacgcagc ttcggactga agccctgacc gaagaagatg
     9901 ttatcgccca gcaccagaca agaaggttca ccattaagga aggtttcacc gatgataaag
     9961 gcctgcgcca gaccgtccgg gctgggctgt tcggcatatt ccagctggat accgaactca
    10021 ctaccatcgc ccagcaggcg ctggaaataa cctttatctt ccggcgtagt gatgatgaga
    10081 atttcgcgga taccggccag catcagcacc gacagcggat agtaaatcat tggcttatcg
    10141 taaatcggca acagttgctt cgatacgccg cgcgtaatcg gatgcaatcg ggtaccggaa
    10201 ccgcccgcca ggataatacc cttcatgttt gcctccgctg aaaattagcc tttcagacct
    10261 aaacgctcac cctgatagct gccgtcctgc acctgcttcc accagctttc attagccaga
    10321 taccactgaa ccgttttacg cattccactt tcaaaggttt cctgcggcag ccagccaagt
    10381 tcacgggcaa ttttcgaagc atcaatggca taacgcagat catgccccgg acggtcagcg
    10441 acaaaggtga tcaggtcacg atagtacgcc acgccgtgcg gcttgttcga agccagttct
    10501 tccagcagct cgcaaatggt ttccacaaca tcgagattct tacgctcgtt gtgaccacca
    10561 atattatagg tttcaccgac tttcccggtg gtcgccacgc aatacagcgc gcgggcgtga
    10621 tcttccacat acagccagtc acggatttgc tgcccgttgc catataccgg cagcggttta
    10681 cccgccagcg cgttgaggat catcagcggg atcagttttt ccggaaagtg gtaaggaccg
    10741 tagttattcg agcagttggt gatcagcgtc ggcagaccgt aggtccgcag ccaggcgcgc
    10801 accagatggt cgctgctggc ttttgacgcg gaataagggc tgctcggcgc atacggcgtg
    10861 gtttcggtga agaaatcatc cgtcgagtgc aggtcaccat atacttcgtc ggtggagata
    10921 tgatgaaaac ggaacgctga ttttttatct tccgtcagcg tattccagta agcccgcgcc
    10981 gcttcaagca atgtataagt cccgacaatg ttggtttcaa taaacgctgc cgggccgtca
    11041 atagaacggt caacatggct ttcggctgcc agatgcatga cacagtctgg ctgatgctca
    11101 gtgaatacgc gtgccagttc tgcccgatcg cagatatcaa ctttctcaaa ggcaaagcgc
    11161 tcgctttgcg cgaccggtgc cagcgacatc aggtttccgg cgtaggtcag cttatcgacc
    11221 actaccaccg catcgctcgt ttcgttgatg atataacgca ccagcgccga gccaataaac
    11281 ccggcaccac ctgttatcag aatttttctc atcagcgcca gactcctttg gcatcgacga
    11341 catactgctg atggacattg tcgccattga taactttgaa ctgactatga tcgaccagca
    11401 tcaccagcac gtctgccgtt gccagcgcct cgtcaagctg cgccagagta caaagcccgg
    11461 tcagtttttt cggcaactgg tggatgttag gctcaacaac cagcgtttcg ccgctgtgcc
    11521 actgggcgat cagttcagcg atttccatcg ccgggctttc gcgcaggtca tcaatattcg
    11581 gtttaaacgc cagaccaaag caggcgattt tcagttcact ggctcgttta tcggtagcag
    11641 caaggcagtc ggcaaccgcc gctttcacct gatcgataac ccagaacggt ttgtgatcgt
    11701 taacttcgcg cgcggtacgg ataagccgcg cctgctgggg gttctgtgcc acgataaacc
    11761 acggatcaac agcaatgcag tgaccgccca cgccagggcc aggctgaaga atattaacgc
    11821 gagggtgacg attcgccagg cgaatcagtt cccagacgtt aatcccctga tcggcacaaa
    11881 tcagcgacaa ttcattagca aaagcgatat tcacatcgcg gaagctgttt tcggtgagct
    11941 tacacatttc cgccgtccgc gagttagtga cgacacactc accttcgagg aaaattttgt
    12001 acaattcgct ggcgcgggcc gaacaaaccg gcgtcatacc accaatcacg cgatcgtttt
    12061 taatcagctc gaccataacc tggcccggta atacacgttc cgggcagtaa gcaatgttga
    12121 cgtccgcctg ctcgcccacc tgctgtggga aagtcagatc cgggcgcatc tctgctaacc
    12181 attctgccat cttctcggtt gaccccaccg gcgaggtgga ttcaaggatc accagcgcgc
    12241 cttttttcag cactggcgca atggagcgag cagccgattc aacgtaggtc atatctggct
    12301 catgatcgcc tttaaacggc gtaggtacag caatcagcca ggcatccgct tcaactggcg
    12361 tcgtgctcgc tcgtaaaaaa ccgccttcta cggcagtttt tactacgctc gccaaatcag
    12421 gctcgacgat atggatttcg ccacgattga tggtatcaac cgcgtgttgg ttgatatcga
    12481 caccaatcac ctgtttttgc cgtgaggcaa acgctgctgc tgtaggcagc ccgatgtaac
    12541 ccagtccgat aacagaaatg gtcgcaaaac tcatagtgat atccgattat tttttaacgc
    12601 ttccagaatg cgagagcatg cctgaccatc accatacggg ttatgggcgc ggctcatagc
    12661 ttgatattcg ttttcgtctt ttaaaagacg cgtcacttcc tcgacaattc gctgcttatc
    12721 cgtgcctacc agacgcaccg tacccgccgt caccgcttcc ggacgctcag tggtatcgcg
    12781 catcaccagc accggtttcc ccagcgaagg cgcttcttcc tgaatgccgc ctgagtcagt
    12841 caaaatcagc caggcgtggt tcatcagcca gacaaacggt aaatactcct ggggatcgat
    12901 cagaatgaca tttttcacat gccccagaat gcgattgacc ggctctctga cgttcgggtt
    12961 gagatgcacc ggatagacaa tctggatgtc ctggtgcgtg gtggcgatgt ctgccagcgc
    13021 gtggcagatt tcttcaaagc cacgaccgaa actctcacgc ctatgaccgg tcaccagaat
    13081 catcttttta tcgggggcga taaacgggta atttgccgcc agttctgaac gcagcgtgtc
    13141 gctgctcatc acctggtcac gcacccataa cagtgcatca atgactgtat taccggtaat
    13201 gaagattcgg ctatccgcaa cgttttcacg cagcaagttt tgccgggaag tttcggttgg
    13261 agagaagtga tacatcgcca gatgcccggt caatgtacgg ttagcctctt ccggccacgg
    13321 cgaatagaga gcgcccgtgc gcagaccagc ctcaacgtga ccaacaggaa tacgctgata
    13381 aaacgccgcc aggctggttg ccagcgtcgt cgtcgtatcg ccgtgaacca gcacgacgtc
    13441 tggtttgaac tcggcaagaa taggttttag cccttccaga atccgacagg ttatctctgt
    13501 caggccctgt cctggctgca ttatgttgag atcgtagtca ggtacaatgg aaaagagttt
    13561 cagtacctga tcaagcatct cccgatgctg cgcagtgacg caaactttag cctcaaaaaa
    13621 aggatctttt gccaacgcat gcaccaacgg agccatcttg atggcttccg ggcgcgtacc
    13681 aaatacagtc agtactttca catcgattct cttcgaataa gcggcgagcg cctttgcgct
    13741 caccgcagca gtgttgctat ttcgagcaac ggcgggttaa tgcgacacca gccccgatca
    13801 gccccccgac aatgccccac ataatcatca ggaaggcacg acgtgggcta tcgcgtttta
    13861 ccggttcttc cggcgtacgc aaatagcgat aggtctgaaa acgcggatcc agggttggac
    13921 caacattcag ggtgtttaac atggctcgat tctgatcata gtcgagatca aaggccggac
    13981 cgacggcctg taaattttcc agtcgagcct ggagcattgg acgcccaagc aggaacattt
    14041 ctgaatcagg taattcctcg gcaggcacat ctgtcgcact gcgcgaaata ttatgctgct
    14101 cagcaatttt cagcgcctgc tcaatactgt tcatccggcg gtcgtagatg gctttcgcca
    14161 cctcttcctg acgcttcacc tgagctttca tctggatggt gcgtgccgcc catgcgcctt
    14221 tcagctcatc attcagatgg ctggctgcac gctggctggc aaaagcaaca tactgacgta
    14281 acaggttatt agcgtcaggc gcggtttcgg caataagctt cacgctgtca ttgaccgcgc
    14341 gagtaaagtc tccggggata aactggatgt tgttaatcat ttcatccagc aacgccgcat
    14401 cggctttgct gttgcccacc atccgctgtt tgtaatagtc ggtttgcagc cagaactctc
    14461 tgcgggtatc ccacgaggcc agttgcataa caaactcttt ataggcttcg tccatgaccg
    14521 atggttgatc ggcagaagcc atgtttgaac ggacatccag gttacgcaaa aattgctgct
    14581 gcgagtaata tccccccagc atattcaccg ttggacgatc ggtaatcgcc gtcgagctcc
    14641 actcctgacg agcaaaaaaa gtatacgcca gcgcgattaa cgcaaacgcc agccccatgc
    14701 caataatcca tagcttccca gcccacaagg tacgaaacaa cccacgaata tccagttcat
    14761 tttcagcgtc ttcggccggt ttcccaggca ttggttgtgt catcacatcc tcatttattt
    14821 ggttaaattg gggctgccac cacgatttct acgcagtctg cgttttacgc gcttaataaa
    14881 gcgagcaact ttccaggcac gcttaatgca atatccatag aggaagaatg ctagcaaaaa
    14941 gagcaccagc atgacccact ccgggacaaa atgagaatat tctgccagca cgccaatgga
    15001 agcgagcagt gctgcggcaa gggtaatcag cacaaacgcc tgacgggaag taaacccggc
    15061 acgcatgatc aaatggtgaa tatgctgacg gtcaggagag aatgggctca tgcctttacg
    15121 caggcgacgg tacataatcg ccaccatatc cattagcgga atggcgatta tccacaaagc
    15181 ggtaaccggg ctgatgggat gggttttgcc ctgggtcgtt tcgagcagga tccagataac
    15241 ggtaaaacca atcagcgtac tgcccgcatc acccataaag actttgtagc ggcgacccag
    15301 gataccaagg ttaagcatga tgtatggcag gatggcggcg atcatcgcaa agcaccagat
    15361 tgcgaggctg gtttgcccgt cgaaccacaa aatcataccg attgctgcaa acgagacgca
    15421 ggacaacccg cccagcaagc catcaatgcc atcaaccatg ttgaacgcat taatggccgc
    15481 ccagacggca aatagcgtca ggaagtaacc aaacggtccg agcaccatct cccaggagcc
    15541 aaagatataa cccaggctac tgagataaag tttgccgaac accatcataa caatgccaac
    15601 agcggcctgt atggtggcac ggatttttac gctgatatca aaacgatcat ccagcgcgcc
    15661 aatgaaaaca agcacaccgg cacaagcgag atagagagat gcatgcggaa tatagtaatc
    15721 gacaattccg aacgtgaagc aaatccctgc gtaaaccgaa atccccccaa cgagaggtat
    15781 caatccctgg tgacgtttgc ggaagtttgg tttatccact aaaccgactt tttttgccac
    15841 cttacgggca aaaaacagaa acagtgtcgt gaataaaaaa atactgatga gatcagtact
    15901 cactgtcagt aaattcacaa tgcatgctct cagagaaaat tattagcaga agtataacca
    15961 cgaagacctt tattcagaag ggaaagtccg ttccgaaccc accagccctg tgcaaaaatc
    16021 aaccatttga tatgcaaatt gcataacatg ccagcaaatt ccaggaattt tcctaacatc
    16081 gccagcgcga cattaagatt acagatataa aagcaaaacg ccacgtaaac acgtggcgtt
    16141 tttggcataa gacaaattta tgagcgtttc atcatttcga agaaatcgtc attggtcttg
    16201 gtcatggcca gtttattaat gaggaattcc attgcatcga tttcgcccat cgggtgaatg
    16261 attttgcgca ggatccacat tttctgcagt tcttcctgag tcgtgagcag ctcttcttta
    16321 cgggtaccgg aacggttgta gtcgatagcc gggaagacgc gtttttcagc gatcttacga
    16381 gagaggtgca gttccatgtt gcctgtacct ttaaactctt cgtaaataac ttcgtccatt
    16441 ttagaaccgg tatcgataag cgccgtcgcg ataatggtca ggctgccgcc ctcttccacg
    16501 ttacgcgccg caccaaagaa gcgtttcgga cgatgaaggg cattggcgtc cacaccaccg
    16561 gtcaacactt tacctgacgc cggaacaaca gtgttgtaag cgcgcgccag acgagtgatg
    16621 gagtcgagca gaatgataac gtctttcttg tgctcaacca ggcgtttggc cttctcgatc
    16681 accatttccg caacctgaac gtggcgagat gcgggttcgt caaaggtaga agcaacaact
    16741 tcacctttta ccagacgctg catctcggtt acttcttccg gacgttcgtc gatcagcaga
    16801 accatcagca cacaatccgg gtggttgtaa gcaatgctct gagcaatgtt ctgcagcagc
    16861 atagttttac cggctttcgg cggtgccaca atcagaccac gctgaccacg accgataggt
    16921 gatgccagat ccagtacgcg agcagttaaa tcttcagtag aaccgttacc acgttccata
    16981 cgcagacgag agtttgcgtg cagcggggtt aagttctcaa agaggatttt gttgcgggcg
    17041 ttttcaggtt tgtcgaagtt aacttcgtta actttcagca gcgcaaaata gcgttcacct
    17101 tctttcggcg ggcgaatctt accagagatg gtatcaccag tgcggaggtt gaaacggcgg
    17161 atttggctag gggaaacgta gatgtcatca ggaccggcga ggtaggagct gtctgcggaa
    17221 cggaggaaac caaatccatc ctgcaatatc tccagtacgc catcaccaaa gatatcttcg
    17281 ccactctttg cgtgctgctt caggatggca aaaataatgt cctgcttacg catgcgagcc
    17341 aggttttcca gccccatatt ttcgccgaga gtgatcagct cagaaaccgg cgtattcttt
    17401 aattcggtaa gattcatagt ggtgtgagtt cttaaacttg gggtaaatct cgaacttaat
    17461 gttgtgaatg gtatggcagg atcatccatg cctgtttacg gtcatcgact catgtctttt
    17521 cgctgcctgg tcacaggaga gtacgcagaa ctgaaacgac aagacggatt gagtgacgag
    17581 ccagaaatct gttcacttcg catttaagat aaaacgggaa gcgttggaaa ttacaagatt
    17641 caaacttaat aaggtatgtt taatacgaag tcaacactaa gttagcatga ctcacgccgg
    17701 gcgtccagtt tttagcgacg gggcacccga acatgaaatt cccttacgcc aggttagcgt
    17761 cgaggaactc tttcaactga cctttagaca gtgcgcccac tttggttgcc gccacttcac
    17821 cgtttttgaa cagcagcaga gtcgggatac cacggatgcc atatttcggc gcagtgccag
    17881 ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc ctgatattcg tcagcgattt
    17941 catccagaat cggggcgatc attttgcacg gaccgcacca ctctgcccag aaatcgacga
    18001 ggatcgcccc gtccgctttg agtacatccg tgtcaaaact gtcgtcagtc aggtgaataa
    18061 ttttatcgct catatataac tccacaggaa taagcctggc gtgttggttt cgttgttggt
    18121 gtaacattaa ccaactaaag gttgacttta tttcaccgga tacgctttcg taaagcaata
    18181 gtaagctgat attctaccac actatgagca aaacacattt aacagaacag aagttttccg
    18241 acttcgccct gcatccgaag gttgtagaag cccttgaaaa aaaagggttt cataactgta
    18301 cgcccattca ggcactggcc cttccgctga cgctggcggg tcgtgacgta gccgggcagg
    18361 cgcaaaccgg taccgggaaa acgatggcgt ttcttacgtc aacgtttcat tatcttctct
    18421 ctcatcctgc gattgccgat cgcaaggtga atcaaccgcg tgccttaatt atggcaccga
    18481 cgcgtgaact tgccgtgcag atccacgccg acgcggaacc gctggcggaa gctaccggcc
    18541 tgaagctggg cctggcttac ggtggtgatg gctatgacaa acagctgaaa gtgctggaaa
    18601 gcggcgttga cattctgatt ggcaccacgg gtcgtttaat tgactacgcc aagcagaacc
    18661 acattaacct cggtgccatt caggtagtgg tactggacga agccgatcgc atgtacgatc
    18721 taggctttat taaagatatc cgctggctgt tccgccgtat gccgcctgca aatcagcgcc
    18781 tcaacatgct gttctccgcc acgctttcgt accgggtacg tgaactggcg ttcgagcaga
    18841 tgaacaatgc cgaatatatt gaagtggaac cggaacagaa aacgggccac cgtattaaag
    18901 aagagctttt ctacccttct aacgaagaaa aaatgcgttt gctgcaaacg ctgatcgaag
    18961 aagagtggcc agacagagcg attattttcg ccaataccaa acaccgttgt gaagagatct
    19021 ggggccacct ggcggcagat ggtcatcgtg tcggtttgtt gacaggcgat gtcgcgcaga
    19081 aaaaacgtct gcgtattctt gatgaattta cccgtggcga tctggatatt ctggttgcca
    19141 ccgacgttgc cgcgcgtggt ttgcatattc cggcagtgac gcacgtcttt aactacgatt
    19201 tacccgatga ctgtgaagat tacgttcacc gtattggtcg tacaggtcgc gcaggcgcaa
    19261 gcggtcactc tatcagcctg gcgtgtgaag agtatgcatt gaatttgcct gctattgaga
    19321 cctatattgg tcactcaatt ccggtaagca aatacaatcc ggacgcattg atgaccgatc
    19381 tgccaaaacc gctgcgcctc acgcgcccgc gcacaggcaa tggtccgcgt cgtactggcg
    19441 ctccgcgtaa tcgtcgtcgt tcaggttaaa taaaatgtca tactcaaaat cattcaaact
    19501 gtatcaagat ggcaagagaa tgaatcccga tgagcttact gtagtaagtg attcgggtga
    19561 aagaatgcag ccaacacaga gacagattga aggatgaaga gtatgggttc cacctcgtcg
    19621 ctgtatgcag ccattgatct cggttcgaat agttttcata tgctggttgt gcgcgaggtg
    19681 gctggaagca tccagacgct gacgcgaatt aaacgcaaag tgcgtctggc tgctggcctg
    19741 aatagcgaaa atgacctctc taatgaagca atggagcgcg gttggcaatg tctgcgcctg
    19801 tttgctgaac gtctgcaaga tatccctccc tcgcaaattc gcgttgtcgc tacggcgaca
    19861 ttacgacttg ccgtcaatgc gggtgatttt attgccaaag cgcaggaaat cctcggttgt
    19921 ccggtacagg tgatcagcgg tgaagaggaa gcgcgtctga tttatcaggg cgttgctcac
    19981 accactggtg gtgccgatca gcgcctggtg gtggatatag gcggtgccag tactgaactg
    20041 gtaaccggca cgggtgcaca aaccacctcg ttgttcagcc tgtcgatggg ctgcgtcacc
    20101 tggctggaac gctattttgc cgatcgtaat ctggggcaag aaaattttga tgctgcagaa
    20161 aaagcggcac gcgaagtgtt acgtccggtt gccgatgaat tacggtatca cggctggaaa
    20221 gtgtgcgttg gtgcttccgg caccgtgcag gcgttacagg aaatcatgat ggcacagggg
    20281 atggatgaac gcattaccct ggaaaagttg cagcaattga aacagcgagc cattcattgc
    20341 ggtcggctgg aggaactgga gattgacggg ctgacgctgg aacgtgcatt agtgttcccg
    20401 agtggtctgg cgatcctgat cgccattttt accgaactga atattcagtg tatgaccctg
    20461 gcgggcggtg cgctgcgtga aggcctggtc tacggcatgt tgcatctggc cgtcgagcag
    20521 gatattcgca gccgtacact gcgtaatatt caacgccgct ttatgatcga tattgatcag
    20581 gcacagcgcg tagccaaagt tgcggctaac ttcttcgatc aggtggaaaa cgaatggcat
    20641 cttgaagcaa taagccgcga tttgctcatc agcgcctgtc agcttcatga aatcggcctg
    20701 agcgttgact tcaaacaagc tccgcaacac gctgcttatc tggtgcgtaa cctggatctt
    20761 cccggtttta cccccgcaca gaagaaactg ctggcgacgc tactgctcaa ccagactaac
    20821 ccggtcgatc tctcatcgct gcatcagcaa aatgccgtac caccgcgcgt cgcagaacaa
    20881 ctctgccgtt tactccgcct ggctatcatt tttgccagcc gtcgccgtga cgatctcgtg
    20941 ccagagatga cattacaggc taaccatgaa ctgttgacct tgacgcttcc gcaaggttgg
    21001 ctaacccaac atccgctggg taaagagatt attgctcagg aaaaccagtg gcagagctat
    21061 gtccactggc cgctggaagt gcattaatga gtaagtgccg gatgcgatgc tgacgcatct
    21121 tctccggcct tgattatttc cctcgttttg ccgccatcat cgctttcaga ttcgccagat
    21181 ggctttgccc tttctgcatc cgttcttcgg cactgaccac tttgcgctcc tgttcccaaa
    21241 tcagatcatc ctgcggcagc tccagcaaaa agcggctcgg ctccgggcgc accagttcgc
    21301 cgtactgacg gcgttcttta cacagcgtaa aggtcaattc cttctgggcg cgggtaatgc
    21361 cgacataggc cagccgccgc tcctcatcga tattatcttc atcgatactg ctctggtgcg
    21421 gcaaaaaccc ttcttccata ccgaccatgt agacataagg aaactccagc ccttttgacg
    21481 cgtggagagt catcagttgc acctgatcca gctcttcttc actctcacca cgctccatca
    21541 tgtcgcgtaa agtaaagcgc gtcaccacct gggtgagcgt catcggctca tccagttcgc
    21601 tgccttccag catctccgtc atccagctaa acagttggtt gacgttcttc atgcgcattt
    21661 cggcagcttt cgtgctgggc gatgtttcgt acagccagga ttcataatcc atgccgtgga
    21721 tcagatcgcg caccgcggca atcggctccc gctccgccag acgctggatt tctgccaacc
    21781 agtgagtgaa gcgggtcaat gcttcataac cacgtccgct aagcgtctga ctcaggccca
    21841 tatcaaagct ggcggtaaac atgcttttat tgcgcgtcat cgcccactca cccagctttt
    21901 tcagcgtcgc cgggccaatc tctcgcttcg gcgtgttaac gatacgcaga aatgcgctgt
    21961 catcgtccgg gttagtcagc acgcgcagat aagccagcaa gtccttgatt tcaggacgag
    22021 agaaaaacga cgtaccacca gaaattttgt acgggatgcg gttttgcatc aggaattttt
    22081 caaacacccg cgactgatag ttaccgcgat aaagaatagc gtaatctttg tactgcgttt
    22141 tattgacgaa gtgatgcgca atcagttcac cagtgacgcg ctcggcttca tgttcttcgt
    22201 tattcgcgct taaaactttt agctctgtgc cgtaacccag ttcggagaac agacgctttt
    22261 caaagacgtg cgggttattg gcgatcagga tgttcgccgc tttcaaaata cgtccggaag
    22321 agcggtagtt ctgctcaagc ttaatcacct tcagcgccgg aaaatcctgg ctcagcagca
    22381 ccaggttttg cggacgtgcg ccgcgccagg agtagatcga ctggtcatcg tcacccacca
    22441 cggtaaagcg cgcgcggctg cccaccagca gtttcaccag ttcatactgg ctggtgttgg
    22501 tgtcctgata ttcgtccacc agcaggtagc gaattttgtt ctgccagcgc tcgcggactt
    22561 cttcattgcg ttgcagaagt agcgtcggca ataaaatcag atcatcgaag tcgagcacat
    22621 tacaggcttt caggtgtgca tcatacagcc cataacaatg ggcaaaaata cggtcccgct
    22681 cgccaatcgc actggctgcc gcctgggacg gtgttttgag atcgttcttc cagttagaga
    22741 tggtcgaaat caactgttgc aggagaactt tgtcatcttc aatcagccct tcggtcaact
    22801 ctttgagcaa cgccagctga tcggtatcgt caaacagcga aaagttcgcc ttcatcccca
    22861 gcgccgcata ctcacgcttg atgatatcca gccccagcgt gtggaaggtt gagatcatca
    22921 gcccatgcgc ctctttgcga cccagcgtct gtccgacacg ctctttcatc tcgcgcgccg
    22981 ccttattggt aaaggtcacc gccgcgatat gtcgcgcctg ataaccgcac ccgcggatca
    23041 gatgggcgat tttattggtg ataacacgag ttttaccgga acccgcaccc gccagcacca
    23101 ggcaggggcc ggtaacgaat tcgacagctt gttgttggcc ggggtttaga cgcataggaa
    23161 aatcgctcaa tgaaagtcgg gaggggaaaa taacagcgtg gtagtatagc cagcctcatc
    23221 cttaccactc aaggcacgat catggcaaaa acagcagcag cactgcatat ccttgtaaaa
    23281 gaagagaaac tggctctgga tcttctcgtg cagattaaga acggtgccga tttcggcaag
    23341 ctggcgaaga aacactccat ttgcccatca ggcaaacgcg gcggtgattt aggtgaattc
    23401 cgccagggtc agatggttcc ggcgttcgat aaagttgtgt tctcttgtcc ggtactggag
    23461 ccaaccggcc cgctgcacac ccagttcggt taccacatca ttaaggtgct gtaccgtaac
    23521 taatagcaag gccttctccg ggagaaggcc ttgaactctg aatcgctatt aacccgcaac
    23581 agcaatacgt ttcatatccg tcatatagcc gcgcagtttc ttacctactt gctcaatcgc
    23641 atggctgcga atcgcttcat ttacatcacg cagctgcgcg ttatctaccg caccttccgg
    23701 aatagcttta cccaaatcgc ccggttgcag ttctgccata aacggtttca gcagcggcac
    23761 acaagcgtaa gagaacagat agttaccgta ctcggcagta tcagagataa ccacgttcat
    23821 ttcatacaga cgcttacgga cgatggtgtt ggcaatcagc ggcagttcgt gcagtgattc
    23881 atagtaagca gattcttcga tgatgccgga atcaaccatg gtttcgaacg ccagttcaac
    23941 gcccgctttc accatcgcga tcatcagtac gcctttatcg aagtactcct gctcgccgat
    24001 tttgccttca tactgcggcg cggtttcgaa ggcggttttg ccggtctctt cacgccaggt
    24061 cagcagtttc ttatcatcgt tggcccagtc cgccatcata ccggaggaga attcgccgga
    24121 gatgatgtcg tccatatgtt tctggaacag cggtgccatg atctctttca gctgttcaga
    24181 caacgcgtaa gcacgcagtt tcgccgggtt agagagacgg tccatcatca gtgtgatacc
    24241 gccctgcttc agcgcttcgg tgatggtttc ccaaccgaac tgaattagtt tttctgcgta
    24301 tgccggatcg gtgccctctt ccaccagctt gtcgaagcac agcagcgagc cagcctgcaa
    24361 cataccgcac aggatggttt gctcacccat caggtcagat ttcacttccg caacgaagga
    24421 cgattccagc acgcccgcac ggtgaccacc ggttgctgcc gcccatgctt tagcgatcgc
    24481 catgccttcg cctttcggat cgttttccgg gtgaacggca atcagcgtcg gtacgccgaa
    24541 tccacgtttg tactcttcac gcacttcggt gccagggcat ttcggcgcaa ccatgactac
    24601 ggtgatgtct ttacggatct gctcgcccac ttcgacgatg ttgaaaccgt gcgagtaacc
    24661 cagcgccgcg ccgtctttca tcagtggctg tacggtgcgc actacatcag agtgctgctt
    24721 gtccggcgtc aggttaacca ccagatccgc ctgtgggatc agttcttcgt aagtacccac
    24781 tttaaaacca ttttcggtcg ctttacgcca ggacgcgcgc ttctcggcaa tcgcttcttt
    24841 acgcagagcg taggagatat cgagaccaga atcacgcatg ttcagaccct ggttcagacc
    24901 ttgtgcgcca cagccgacga tgactacttt tttaccctga aggtagctcg cgccatcggc
    24961 gaattcatcg cgccccataa agcgacattt gcccagctgt gccagctgct gacgcagatt
    25021 cagtgtattg aagtagttag ccatggtgat tcctcgtgat gttgtgcttc ttattgttcg
    25081 gttcgcgttt gcgagattga attcactata tgacaggaaa tttattgcgg aaattgatat
    25141 attcacaacg tcacattgca atttctgcaa cgtcaacatt gagggctgcg ccattggatt
    25201 tacgcgatct gaaaaccttc ctgcatctgg cggaaagccg ccattttggc cgcagcgcgc
    25261 gggcgatgca cgttagcccg tccacgctct cacggcagat tcagcgcctg gaagaagatc
    25321 tcggccagcc gctgtttgtg cgcgataacc gcacggtgac gctgactgaa gcgggcgaag
    25381 aactgcgcgt tttcgcccag caaacgctgt tgcagtatca gcagctgcgc cacaccatcg
    25441 accagcaagg gccgttgctc tatggcgaat tacatatctt ctgctcggtg accgccgctt
    25501 acagccatct gccgccgatt ctggatcgct tccgcgcgga acacccgtcg gtggagatca
    25561 aactcactac cggtgatgcg gcagatgcaa tggaaaaagt cgtcacaggt gaagctgatc
    25621 tggcgattgc cgggaagcca gagactctgc ccggcgcggt ggcgttttcg atgttggaga
    25681 atctggcagt ggtgttgatt gcacccgcat tgccctgccc ggtgcgtaat caggtttctg
    25741 tcgaaaagcc ggactggtca acggtaccgt ttatcatggc caatcagggg ccggtgcgcc
    25801 gccgcattga gttgtggttc cgacgcaata aaatcagtaa cccgatgatt tacgccacgg
    25861 ttggcgggca tgaagcgatg gtatcgatgg tggcgctcgg ctgtggcgtg gcattgttgc
    25921 cggaagtggt gctggaaaac agccccgaac cggtgcgtaa ccgcgtgatg attttagagc
    25981 gcagcgatga gaaaacgccg tttgagctgg gcgtgtgtgc gcagaaaaag cggctacatg
    26041 agccgctaat tgaggcattc tggaagattt tgccgaacca ttaacccgcc aaaaagaacc
    26101 tgaacgccgg gttattggtt tcgtcgtggc aatcgtagcc cagctcattc agccgggttt
    26161 cgaaatccgg ttcatggtcg ccaagttcga acgccgccag tacgcgcccg tagtcggtgc
    26221 catggctgcg atagtggaac aaagaaatgt tccagtgcgt acccagcgtg ttgaggaagc
    26281 gcagcagcgc gcccggtgat tccgggaatt cgaagctgta gaggcgttcc tgcaacggat
    26341 gtgacggacg cccgccgacc atatagcgca catgcaactt cgccatttcg tcgtcggaga
    26401 gatccaccac gctgtagccg ccgtcgttga gcatctgcaa aatttctttg cgctcttcga
    26461 ggccgcggct caggcgcacg ccgacaaaga tgcaggcgtt tttggcatcg gcaaaacggt
    26521 agttgaactc ggtgaccgat cgcccgccaa gcagttggca gaatttgagg aagctgcctt
    26581 tttcttccgg aatggtcacc gccaacaacg cttcacgctg ttcgcccagt tcgcagcgtt
    26641 ccgagacgta gcgcaggccg tggaagttca cgttggcacc ggaaagaata tgcgccagcc
    26701 gttcgccgcg aatgttgtgc tgggcgatat attttttcat tcccgccagc gccagcgcgc
    26761 cagagggttc cgccaccgca cgcacatctt cgaataaatc cttcatcgcc gcacagatcg
    26821 catcgctatc gacggtgatg atgtcgtcga gatactcctg gcataaacgg aaggtttcgt
    26881 caccgatgcg ttttaccgcg acaccttcgg cgaacagtcc cacgcgtggc agatcaaccg
    26941 gatgacccgc atccagcgct gctttcaggc aggcggagtc ttccgcttct acggcgatca
    27001 ctttgatttg cggcatcagt tgtttgatca gcaccgctac gcccgccgcc agaccgccgc
    27061 cgccgactgg cacaaatacg cggtcgagat gggcgtcctg ctggagcagt tccagcgcca
    27121 gcgtgccttg cccggcaatc accatcggat ggtcgaacgg cggcacccag gtgaacccct
    27181 gctgctgtga cagttcgatc gctttagctt tcgcttcgtc aaagttcgcg ccatgaagca
    27241 gcacttcgcc gccgaatcca cgtaccgcat cgactttgat gtcggcggtg gcggttggca
    27301 taacgatcag ggccttcacg cctaaccgcg cagaagaaaa cgcgacgccc tgcgcgtggt
    27361 tacccgcaga agcagtgatc acgccgtgcg ctttttgttc ttccgtcagg cccgccatca
    27421 tggcgtatgc gccgcgcagc ttaaagctgt gcactggctg gcgatcttcg cgcttcacca
    27481 gaatgacgtt atcaagacgc gacgacagtt tttccatttt ttgtagcggc gtaacctgcg
    27541 ccgcctcgta aaccggcgcg cgcagcactg ctcttaaata ttcggcacct tccggagcac
    27601 cggacagggg ttgcgagtca gccattatta accccccagt ttcgatttat cgcgcaccgc
    27661 acctttgtcg gcgctggttg ccaggctggc gtaggcgcac agggcaaagg aaacctgacg
    27721 ttcacggttt ttcggcgtcc aggctttgtc accccgagct tcctgcgctt cacgacgcgc
    27781 cgccagttcg gcatcgctta cctgtaactg aatgccacgg ttcgggatgt cgatagcgat
    27841 caggtcacca tcttcaatca gaccaatgct gccgccgctt gccgcttccg gtgagacgtg
    27901 gccgatggaa agaccagagg tgccgccaga gaaacgaccg tcggtgatca gcgcacaggc
    27961 tttaccgaga cccattgatt tcaggaagct ggttgggtag agcatttcct gcatgccagg
    28021 gccgcctttc gggccttcat agcggatcac caccacatcg cccgcgacaa ctttgccacc
    28081 gaggatcgct tctaccgcgt cgtcctggct ttcgtacact ttcgccgggc cggtgaattt
    28141 gaggatgctg tcatcgacgc ccgcggtttt cacgatgcag ccgttttcag caaaattacc
    28201 gtagagcacc gccaggccgc cgtctttgct gtaggcgtgt tccagcgagc ggatacagcc
    28261 gtttgcgcga tcgtcatcga gagaatccca acggcaatcc tgcgagaatg cctgtgtagt
    28321 acgaatgccc gcagggcctg cgcggaacat attttttacc gcgtcatcct gggtcagcat
    28381 aacgtcgtat tgttccagag tttgcggcaa cgtcaggcca agtacgtttt tcacatcacg
    28441 gttcagtaac cccgcgcgat ccagttcacc gagaataccg ataacaccac cagcacggtg
    28501 aacatcttcc atatggtatt tttgggtgct cggcgcaact ttgcataact gcgggacttt
    28561 acgcgaaagt ttgtcgatat cactcatggt gaagtcgatt tccgcttcct gcgccgccgc
    28621 cagcaggtga agtaccgtgt tggtagagcc gcccatcgcg atatccagcg tcatggcatt
    28681 ttcaaacgcc gctttgctag caatattgcg cggcagtgca ctttcgtcgt tttgctcgta
    28741 gtaacgtttg gtcaattcaa caatgcgttt accggcattg aggaacaact gcttacggtc
    28801 cgcgtgggtt gccagcagcg agccgttgcc cggctgcgac aggcccagcg cttcggtcag
    28861 gcagttcatt gagttagcgg taaacatccc ggagcaggag ccgcaggtcg gacacgcgga
    28921 acgttcaacc tgatcgctct gggagtcaga aactttcggg tctgcgccct ggatcatcgc
    28981 atcaaccaga tcgagtttga ttatctgatc ggacagtttg gttttcccgg cctccatcgg
    29041 gccgccggaa acaaagatca ccggaatatt caggcgcagg gaagccatca gcatccccgg
    29101 ggtgattttg tcgcagttag agatgcagac catggcgtcg gcgcagtggg cgttgaccat
    29161 atactcaacg gaatcagcga tcagttcgcg agatggaagt gaataaagca tccccccgtg
    29221 gcccatggca atcccatcat ccaccgcaat ggtgttgaac tctttggcaa cgccgccagc
    29281 cgcttcaatt tgttcggcga ccagtttacc gagatcgcgc agatggacgt gacccggtac
    29341 aaattgggtg aacgagttca caaccgcgat aatcggcttg ccaaaatcgg cgtcggtcat
    29401 tccggtggcg cgccacagcg cacgagcacc cgccatatta cgaccatgag tggtggtggc
    29461 ggaacggtac ttaggcatac tttatttact cccagtgtct gtctcgtaaa tgggacggtg
    29521 cgtgccgtcc cattttttgt atttattgat taacttgatc taaccagccc catttatctt
    29581 cggtttcgcc agtgaagagg ccgaagaagg cttgctgaat gcgtttggta accgggccac
    29641 aacggccttc gccaatctga ataccgtcta cgctgcgcac tggcgtgatt tctgccgccg
    29701 taccggacat aaagacttca tccgccaagt acagggattc gcgcgacagc acctgctcac
    29761 gcacttcaat tcccagctct ttcgccagtt tgatgatggc atcacgggta atacccggca
    29821 gcgcggagga ggtgaacggt ggggtgaaca gcacaccatc tttcacttca aacaggtttt
    29881 cgcctgcgcc ttcagagata taaccgttca catccagcgc gataccttcc tgataaccgt
    29941 ggcggcgcgc ttcgctaccc accagcaggg aagagagata gttaccaccg gcttttgccg
    30001 cagttgggat ggtgtttggt gctgcgcggt tccaggagga aaccatcgca tcgatcccct
    30061 gctccagcgc ttctgcgccc agatacgctc cccacgggaa agcggcgata atcacatcgg
    30121 ttgagtatcc cgctggcggg tttactccca tgccaacatc accgacgaag atcagcggac
    30181 ggatataggc gctggtgaga ttgtttttgc ggatcacgtc acgacaagct tccatcagct
    30241 catcaatgct ctgtgaaacc gggaagcgat agattttggc ggagtcatgc agacgctgca
    30301 tatgctcacg atggcggaat acaaccggtc ctttgtgtga gtcgtagcaa cggatgcctt
    30361 caaaaaccga ggtgccatag tgcagcgcgt gcgacatcac atgcaccttc gcgtcttccc
    30421 agcgaaccat ctccccattg aaccaaatgt aatcagcttt cttcgtggtc atttttatat
    30481 tccttttgcg ctcaggcgcg gatttgttgt gatgtggttg tgctctggca gatggcaacg
    30541 tgtgcgacgt ccaccagttt atttaactga ctaaacagta agtcgaccga ccgtgggctg
    30601 gcaacggtca attcgatatt tatattttgt gcatcgctgg cggcagccat attcattgag
    30661 cagacgtgga aaccacgatg acgcaccacg cgtaaaacac gttctaaggt ttccggattg
    30721 aagcgagccg atacattgac ctgatgttgc atcatgataa tttctccaac atttctgaat
    30781 tactggcacc tggcggcacc agcggccaga cgttctcaag ttcgtcgatt gagacatgaa
    30841 gcaggtatgg cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc tggtctttac
    30901 gggtgatgtg ttggccaggg atgccgaagg cgctggctaa catgaggaaa tcggggttat
    30961 cggtaagggt ggtttcgctg tatcgttcct gaaaaaacag ttgctgccat tgtcgaacca
    31021 tccctaaccg ttggttatcg agtaagacga ttttcaacgg taactgcttg cgttttacgg
    31081 tgcccagctc ttgcacattc atcatgaaag agccgtcacc ggagatacag acaacggtat
    31141 cgttcggtcg cgcgacttgt gcgccaactg ccgccggtaa accaaaaccc atggtaccta
    31201 agccgctgga ggtgatgaaa ttttccgggc gagtgtgggc gatgtgctgc gcagcccaca
    31261 tctggtgctg ccccacatct gtggtcacga cgcaatccgc aggtttacga tccgacagtt
    31321 gttttaacaa caacggagcg tagatagcgt caccgggatg gtcgtaacgc caggaatgtt
    31381 catcacgcag ctgcgcgcag tgttgctgcc agtcattgat atttaacggc tgctgtaatg
    31441 ctggtaacag agcatttaaa tcaccttgta atgccacatg tgcctgacgc agcttgttca
    31501 tttctgccgg gtcgatatcc atatggataa cactggcgtg tggtgcgaag gtgttcagtt
    31561 tgccggtcac ccggtcatca aaacgtgcac ccacggcgat cagcaggtca cactcctgca
    31621 ccgcgaagtt tgccgctttg gttccgtgca tccccagcat gcccagatag tacggataat
    31681 ctgcttctac tgcgcccagc cctttcagcg tacaggtggc aggcattttt gtggcagcga
    31741 gaaattcacg caaagccgga actgcctgcg ccatccccac gccaccgcca acgtacagca
    31801 tcggtttttg cgcttttgcc agcatctggc gcgcttgctc gacttcggca tgtgggaaag
    31861 tcacttcgtt ttcaacggtg gtgaaccacg gttccagatc gccgctggct aactggatat
    31921 cttttgggat atcgaccaga accggaccag gacgacctga gctggcaacg tcgaatgctt
    31981 cagccatgat gcgcggcaac tcttccagag actgcaccag aaagctgtgc ttggtacagg
    32041 ctaacgacaa tcccaggaca tccacttcct gaaatgcgtc ggtgccgata aacggtgcgg
    32101 acacctgacc agtgatggca acgacaggga tggaatctaa cagtgcgtcc gcaagcccgg
    32161 ttatcaggtt ggttgcgccc ggaccagacg tggcgataca tacgccagtt ttaccggtag
    32221 cacgggcata accgatagcc gccattgccg caccctgctc atgtcggcac agcaagtgct
    32281 ccacgccgcc gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc ggataaccga
    32341 aaacggtgtt cacaccctgt gcccgcaacg catgtaccac ccactgtgcg ccattcatag
    32401 ttagttcccc gtcctgaatc ttgagaaaca gaattttgtg ctgttattca ttgtctgctc
    32461 ctcggttatg tttttaaggt caaaaaaacc cccggacctt tcggtgcggg ggtcttagtt
    32521 cgttaaggct tgatctctaa gcctttcctc gtccaagtgc agccccgcac ggtgggataa
    32581 taatcaccac cacgctaatc acgaccaggc taatcactcg tagaagggct gtcattttgt
    32641 cttttcttgc atcttgttcg aaggaatgcc taaagagtta ccatagattt tgcgaatagc
    32701 acaagatatt tttgggccta tttttatgat ggctttaata atttcaatag aaattgttgt
    32761 tttttaaaaa gaaaatagtg agtgaaaaaa ttcaatttca gtataaatgt tgttcccttc
    32821 ctcgtagttc atcctttgtc cgctgcattt ttgcgagcat cattccaccg ccagaacaac
    32881 tcaacttcac ttgcacaaat aaacaggaat cagccactaa aattcacgaa ttagcgggct
    32941 acagcagcga tatcttatgc acataatggt gactcaagga gggtttatgt cactgtcaat
    33001 tgttcatacc cgcgcagccc tggaagtaaa tgcgccccca atcactgttg aggtacatat
    33061 cagtaaaggt ctacccggct taacgatggt gggcttaaca gaaacaacgg tgaaagaagc
    33121 tcgcgatcgc gtgcgcagcg ccattatcaa tagcggatat gaatatccgg cgaaaaaaat
    33181 caccatcaac cttgctccag ccgatctgcc aaaagaaggg ggacgatatg atttacctat
    33241 cgccattgcg ttgctggcgg cctcagaaca gcttacagcc aataagttag atgaatatga
    33301 attagtcgga gaactagcgc ttacaggcgc tctgcgtggc gttcccggtg caatctccag
    33361 tgcaactgaa gctattaagt cgggcagaaa aattatcgtc gcgaaagata acgaagatga
    33421 agtggggcta attaacggtg aaggatgcct gatagccgat catctgcagg ctgtctgtgc
    33481 gtttctggaa ggtaagaaca ctctcgaacg cccgaaacca actgatgcag tatcccgggc
    33541 gctacaacat gatctcagtg atgttgtcgg tcaggagcaa ggaaaacgag gactggaaat
    33601 taccgccgct ggtgggcaca accttttact gattgggccg ccgggaacag gtaaaacaat
    33661 gctcgccagc cgtattaatg gcctgttgcc agatttaagc aatgaagagg cactggagag
    33721 cgctgcgata ttaagtctgg taaatgctga atcagtacaa aaacaatggc ggcagcgccc
    33781 gttccgctca cctcatcaca gtgcgtcgtt aactgcgatg gtgggcggtg gtgcaattcc
    33841 agggccaggt gaaatttcgt tggcgcataa cggcgtgctt tttcttgatg agctacctga
    33901 atttgaacgg cgtacactgg atgccttgcg agagccgatt gaatccgggc agatccatct
    33961 ttcacgcaca cgagcaaaaa taacctatcc agcccgtttc cagcttgttg cggcgatgaa
    34021 tcccagccct accggacatt atcagggaaa ccataaccgc tgcacgccag aacagacatt
    34081 acgttatctc actggagatc ccattaccac cccccggcat tttgagtaaa acggtagtgc
    34141 cgggagaaag cagcgccacc gttaaacaac gcgtaatggc cgccagagag cgccaattta
    34201 agcggcagaa taaactgaat gcctggctgg atagtccgga aatacgccaa ttctgcaaac
    34261 ttgagagcga agatgcgatg tggttggaag aaacgctgat ccatctgggg ttatcgattc
    34321 gtgcctggca gcggttactg aaagttgcac gaaccattgc tgatattgat cagtctgaca
    34381 ttatcacacg tcagcatttg caggaggcag ttagctatcg tgcgattgac cgtttgctca
    34441 tccatctgca aaaactactg acataaaaaa agggcatttc gcccttttta ttaatcgtca
    34501 gaatcggtgt agtcttcagc accttcaacc tgcggtttac cgccggaaag ggtgtgaaaa
    34561 cgttttggac gcttaatacg cgtcatatac ttggaccaca cgcgttctgc ttctgtcact
    34621 ggctcacgtt cgccacggca tactgctacg aagagtttct cttcctcggt aaccggctcg
    34681 cgtttgccaa gatccaactc attgaaggca taaccatgac gctcaagcag ttgtgcctct
    34741 ttgatggtga aatcaccatg acgagagaat ccacgtggat aatgtttatt gtcgaaatat
    34801 cgattagtcg tcgtaaagct ttccgccatc ctgcacgctc ctaattcttt gaccgagcta
    34861 gttatggcgc ggagtattag ttacgcttga cagagtgtaa aacaaaacat ttaaatcata
    34921 acgacaaata attttgcgga gagcactgtg gatacggaat tgttaaaaac tttcctggaa
    34981 gttagccgaa cgcgtcactt tggtcgagcg gctgaatcgc tctatctgac ccagtcagca
    35041 gtgagctttc gaatcagaca actggaaaat caactgggtg tgaacctttt cacccgccac
    35101 agaaacaata tccgtttaac cgctgccggt gaaaaactac tgccttatgc agaaacgctc
    35161 atgagcacct ggcaggccgc ccgcaaggag gtggcgcata cctcacgaca taacgagttt
    35221 tctatcggtg ccagcgcctc gttgtgggaa tgtatgctta atcagtggtt gggacgcttg
    35281 tatcaaaatc aggatgccca tacaggctta cagttcgaag cgcgaattgc ccaacggcag
    35341 tctctggtaa aacagctgca tgaacgccag cttgatcttc ttattaccac tgaagcgccc
    35401 aaaatggacg aatttagtag tcagttgctg gggtatttca ctttagcgct ttataccagt
    35461 gccccttcta aactaaaggg agatcttaac tatctgcgac tggagtgggg gccagatttt
    35521 caacagcatg aggcaggttt gatcggtgct gacgaagttc ccattctgac aaccagttct
    35581 gctgaactgg cacagcaaca gattgcgatg cttaatggtt gcacctggct acccgtcagc
    35641 tgggcgcgta aaaaaggcgg cctgcatacc gttgtcgata gcacaacact ttcacggccg
    35701 ctttatgcca tatggctgca aaatagcgat aaaaatgcgt tgattcgcga tcttttgaaa
    35761 attaacgtgc tggatgaagt gtattaatct gaatggctgg caaggatgcc ggtagaagga
    35821 tttacttcgg agaggattat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
    35881 tctggcaggg gcggagagac tcgaactccc aacacccggt tttggagacc ggtgctctac
    35941 caattgaact acgcccctaa ttagggtggc ggaacggacg ggactcgaac ccgcgacccc
    36001 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa
    36061 cc
//
LOCUS       DAJTYH010000031        35416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTYH010000031 DAJTYH010000000
VERSION     DAJTYH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..235
                     /locus_tag="OO609_001529"
     CDS             <1..235
                     /locus_tag="OO609_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6632995.1"
                     /translation="RGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRP
                     LIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(282..1040)
                     /gene="deoR"
                     /locus_tag="OO609_001530"
     CDS             complement(282..1040)
                     /gene="deoR"
                     /locus_tag="OO609_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6632996.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1098..1694)
                     /gene="ybjG"
                     /locus_tag="OO609_001531"
     CDS             complement(1098..1694)
                     /gene="ybjG"
                     /locus_tag="OO609_001531"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6632997.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1979..3205
                     /gene="mdfA"
                     /locus_tag="OO609_001532"
     CDS             1979..3205
                     /gene="mdfA"
                     /locus_tag="OO609_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6632998.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3246..3530)
                     /locus_tag="OO609_001533"
     CDS             complement(3246..3530)
                     /locus_tag="OO609_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6632999.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3616..4431)
                     /gene="ybjI"
                     /locus_tag="OO609_001534"
     CDS             complement(3616..4431)
                     /gene="ybjI"
                     /locus_tag="OO609_001534"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6633000.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4431..5639)
                     /locus_tag="OO609_001535"
     CDS             complement(4431..5639)
                     /locus_tag="OO609_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633001.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5723..6259
                     /gene="rcdA"
                     /locus_tag="OO609_001536"
     CDS             5723..6259
                     /gene="rcdA"
                     /locus_tag="OO609_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6633002.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6434..8119)
                     /locus_tag="OO609_001537"
     CDS             complement(6434..8119)
                     /locus_tag="OO609_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6633003.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8389..8766
                     /locus_tag="OO609_001538"
     CDS             8389..8766
                     /locus_tag="OO609_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6633004.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8796..9053)
                     /gene="grxA"
                     /locus_tag="OO609_001539"
     CDS             complement(8796..9053)
                     /gene="grxA"
                     /locus_tag="OO609_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6633005.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9213..9500
                     /locus_tag="OO609_001540"
     CDS             9213..9500
                     /locus_tag="OO609_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6633006.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9484..10206
                     /gene="nfsA"
                     /locus_tag="OO609_001541"
     CDS             9484..10206
                     /gene="nfsA"
                     /locus_tag="OO609_001541"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6633007.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10267..11169
                     /gene="rimK"
                     /locus_tag="OO609_001542"
     CDS             10267..11169
                     /gene="rimK"
                     /locus_tag="OO609_001542"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6633008.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11257..11733
                     /locus_tag="OO609_001543"
     CDS             11257..11733
                     /locus_tag="OO609_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6633009.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12085..13197
                     /gene="potF"
                     /locus_tag="OO609_001544"
     CDS             12085..13197
                     /gene="potF"
                     /locus_tag="OO609_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6633010.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVHENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13292..14425
                     /gene="potG"
                     /locus_tag="OO609_001545"
     CDS             13292..14425
                     /gene="potG"
                     /locus_tag="OO609_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6633011.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14435..15388
                     /gene="potH"
                     /locus_tag="OO609_001546"
     CDS             14435..15388
                     /gene="potH"
                     /locus_tag="OO609_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6633012.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15385..16230
                     /gene="potI"
                     /locus_tag="OO609_001547"
     CDS             15385..16230
                     /gene="potI"
                     /locus_tag="OO609_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6633013.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16290..16778
                     /locus_tag="OO609_001548"
     CDS             16290..16778
                     /locus_tag="OO609_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6633014.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16819..17946
                     /gene="rlmC"
                     /locus_tag="OO609_001549"
     CDS             16819..17946
                     /gene="rlmC"
                     /locus_tag="OO609_001549"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6633015.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18121..18852)
                     /gene="artJ"
                     /locus_tag="OO609_001550"
     CDS             complement(18121..18852)
                     /gene="artJ"
                     /locus_tag="OO609_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6633016.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19144..19812)
                     /gene="artM"
                     /locus_tag="OO609_001551"
     CDS             complement(19144..19812)
                     /gene="artM"
                     /locus_tag="OO609_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6633017.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19812..20528)
                     /gene="artQ"
                     /locus_tag="OO609_001552"
     CDS             complement(19812..20528)
                     /gene="artQ"
                     /locus_tag="OO609_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6633018.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20535..21266)
                     /gene="artJ"
                     /locus_tag="OO609_001553"
     CDS             complement(20535..21266)
                     /gene="artJ"
                     /locus_tag="OO609_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6633019.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21284..22012)
                     /gene="artP"
                     /locus_tag="OO609_001554"
     CDS             complement(21284..22012)
                     /gene="artP"
                     /locus_tag="OO609_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6633020.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22230..22745)
                     /locus_tag="OO609_001555"
     CDS             complement(22230..22745)
                     /locus_tag="OO609_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6633021.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23394..25163
                     /locus_tag="OO609_001556"
     CDS             23394..25163
                     /locus_tag="OO609_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6633022.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25374..25697
                     /locus_tag="OO609_001557"
     CDS             25374..25697
                     /locus_tag="OO609_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6633023.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25694..26524
                     /gene="amiD"
                     /locus_tag="OO609_001558"
     CDS             25694..26524
                     /gene="amiD"
                     /locus_tag="OO609_001558"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6633024.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26521..27534)
                     /locus_tag="OO609_001559"
     CDS             complement(26521..27534)
                     /locus_tag="OO609_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6633025.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27633..29063)
                     /locus_tag="OO609_001560"
     CDS             complement(27633..29063)
                     /locus_tag="OO609_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6633026.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29074..30075)
                     /gene="ltaE"
                     /locus_tag="OO609_001561"
     CDS             complement(29074..30075)
                     /gene="ltaE"
                     /locus_tag="OO609_001561"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6633027.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30112..31860)
                     /gene="poxB"
                     /locus_tag="OO609_001562"
     CDS             complement(30112..31860)
                     /gene="poxB"
                     /locus_tag="OO609_001562"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6633028.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGT
                     PTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLM
                     GDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITG
                     IRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIIS
                     GRGDEVIELAKTNWLR"
     gene            complement(31993..32961)
                     /gene="hcr"
                     /locus_tag="OO609_001563"
     CDS             complement(31993..32961)
                     /gene="hcr"
                     /locus_tag="OO609_001563"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6633029.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32973..34625)
                     /gene="hcp"
                     /locus_tag="OO609_001564"
                     /gene_synonym="priS"
     CDS             complement(32973..34625)
                     /gene="hcp"
                     /locus_tag="OO609_001564"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6633030.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34769..>35416)
                     /gene="lysO"
                     /locus_tag="OO609_001565"
     CDS             complement(34769..>35416)
                     /gene="lysO"
                     /locus_tag="OO609_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6633031.1"
                     /translation="RGLPWRNHHQQEKLPSRIAMALESLKLCGVVVIGFAIGLSGLAF
                     LQHATEASEYTLILLLFLVGIQLRNNGMTLKQIVLNRRGMIVAVVVVVSSLIGGLINA
                     FILDLPINTALAMASGFGWYSLSGILLTESFGPVIGSAAFFNDLARELIAIMLIPGLI
                     RRSRSTALGLCGATSMDFTLPVLQRTGGLDMVPAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8778 a         8773 c         9109 g         8756 t
ORIGIN      
        1 gcgtgggcag ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc agcttaccgc
       61 accgctgaaa aaaggtcagg ttgtcgggac cattgatttc cagcttaacg gtaaatccat
      121 tgagcagcgt ccgctgatcg tgatggaaaa tgtggaagag ggcggattct ttggtaggat
      181 gtgggatttc gtgatgatga aattccatca gtggttcggc agctggttct cttaatcttc
      241 tgataaccgg atggcgcgaa acgtcatccg gttatacgtc attaatacat caacttaata
      301 cggtgcgtct gcgcgtactt cacatactca tcttccgggc aacaatcact caccacaata
      361 tcaaagcgtt tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt actgtggtcg
      421 acaaccagca catgcttttg cgccatcgac atggcccagt gttttaccgg caactcttca
      481 agattaaaac aggtagcgcc tttactgaca tgcacgcccg ccgcggaata aaaagcgata
      541 tccgggcaaa aattattcaa cgtttgctga aaatcgatgg gtttgaaaat ggcgttgctg
      601 gcgtgaaatt caccaccgca cagaaatgcg cggcaatggg gtttctcttt cagcgccaga
      661 aaggtattta gcgaataaca aacggcggta aaagggattt cattatcaat cgcttcaata
      721 atccacggcg tggtggtgcc acagtcaaaa aagagggtct gatcgggttc taccagtgtc
      781 gcagccagtt ttgccgcccg gcgtttttct tccaccaggc gggatttttg atcgcttaac
      841 aggtaatggc tggcactgcg cggttccaga acaatatagc cgccgagcaa aacgacgggc
      901 gcactgtggt tgttcagatc gcgacgaatc gtcatctccg aaaccccaag cagggcggcg
      961 gcgtctttaa gatgtaactt atcgctgcgt tttaattctt gcagcagctg cccgatacgc
     1021 tcttcgcgac gtgtttccat aatccctctg aatagttatt gaagcgagcc gctcaatact
     1081 acacttttta gcagagatca gtcacgcacc cagcctttgc ggatcggtaa tgcaaaacag
     1141 acgcgatacc acgattgcag acgttgatag agtttatgcc ccatcgcttg ccagataatc
     1201 tgggcactaa ggcagccaat catacctgcc agcaatccac cgagcatatc cagcggccag
     1261 tggacgccaa gataaacgcg cgaccaggca atgacgacgg ccagcaccat taaaagtgag
     1321 ccggaccaca ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat caccgtaccg
     1381 tgatcgctcg ggaatgagtc atccgccgca tgatgcagga agttatagcc gatattttcg
     1441 acaaagggtc ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa caggctgacc
     1501 gccagcgcga tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc ccacaaccaa
     1561 agtaccgcgg ccagcaacgg caccacggta atcaaatctt tagcaataaa aatcgccaac
     1621 gagatcatcc acggagccga gtctggcgtc gcgttaataa gagagaatag agagagattc
     1681 aaattttcca gcataacttc ccgacgcaaa gtgattaaaa gggagccaat acaggcaagt
     1741 cgttgagaat aaagtgcagg ttaaactggg taaagcggca tcgtcttatt tccctcaagc
     1801 ggcctgttta cggtgggtga ttgtaacggg cataggttaa ataaaactta aagaaagcgt
     1861 agctatactc gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac aaaattcgct
     1921 ttctcgttag ctgcgctttt attaaactct gcgcgattat tattggcgaa gaaattgcat
     1981 gcaaaataaa ttagcttccg gtgccaggct tggacgtcag gcgttacttt tccctctctg
     2041 tctggtgctt tacgaatttt caacctatat cggcaacgat atgatccaac ccggtatgtt
     2101 ggccgtggtg gaacaatatc aggcgggcat tgattgggtt cctacttcga tgaccgcgta
     2161 tctggcgggc gggatgtttt tacaatggct cctggggccg ctgtcggatc gtattggtcg
     2221 ccgtccggtg atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg caatattact
     2281 ggcgcaaaac attgaacaat tcaccctgtt gcgcttcttg cagggcataa gcttctgttt
     2341 cattggcgct gtgggatacg ccgcaattca ggaatccttc gaagaggcgg tttgtatcaa
     2401 gatcaccgcg ctgatggcga acgtggcgct gattgctccg ctacttggtc cgctggtggg
     2461 cgcggcgtgg atccatgtgc tgccttggga ggggatgttt gttttgtttg ccgcattggc
     2521 agcgatctcc tttttcggtc tgcaacgagc tatgcctgaa accgccatgc gtataggcga
     2581 gaaactgtca ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga acggccgctt
     2641 tgtggcgggg gcgctgggat tcgttagcct gccattgctg gcgtggatcg cccagtcgcc
     2701 gattatcatc attaccgggg agcagttgag cagctatgaa tatggcttgc tgcaagtgcc
     2761 tattttcggg gcgttaattg cgggtaactt gctgttagcg cgtctgacct cgcgccgcac
     2821 cgtacgttcg ctgattatta tgggcggctg gccgattatg attggtctgt tggtcgctgc
     2881 tgcggcaacg gttatctcat cgcacgcgta tttatggatg accgccgggt taagtattta
     2941 tgctttcggt attggtctgg cgaatgcggg actggtgcga ttaaccctgt ttgccagcga
     3001 tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg caaatgctga tctttaccgt
     3061 cggtattgaa atcagcaaac atgcctggct gaacgggggc aacggactgt ttaatctctt
     3121 caaccttgtc aacggaattt tgtggctgtc gctgatggtt atctttttaa aagataaaca
     3181 gatgggaaat tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga tcaggcattc
     3241 tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc acctacgctg
     3301 gcaagatggc gaacataagg atgcgggcgg taagccggag ctggcgcagg agccacatag
     3361 acggtttgcg gcacagtcgc gacgctgacc gcttgtggaa cgctgactga atcaggaacc
     3421 acaaccactt tgtatccact cggcacatcg acggtgacac tttgcgccat cgccacacca
     3481 gtaaagccca ttaaaagcgc gcccaacagt agacaatttt tcataatcat ttcgcctgaa
     3541 ataatgcagc gggttaattt ccgcgaatta tgcagccttc attcaggcat tgtgttgccg
     3601 ggcgtgctgc cgtttttatt ggtcaaatgg cgcttcgtgt ttaagaactt tatcgatcac
     3661 atccagtacg ccttcacggt tattgtagcc tgcccggtat tttgccgctg cgacgactgc
     3721 gctgccggca ttttccattg caaaactaaa gcctgcctga cgcagcatct caatatcgtt
     3781 accgccatcg ccaaagacca ccacttcgct gtcgtctatt ccccataatt tctgcagttg
     3841 gcgaaggcca ttggctttat gtacgccggg gataatcaga tcgatgctgc cgttgccggt
     3901 gtggactggc accataatat cgccgatggc ctcatgtaat gctttttgta cttgtggaat
     3961 cagttcatcg gaaagattca ggccaaactt aaagaaaata tcttctaagt tgtcaaagtt
     4021 atcgacgtat tccagacggt gataatacat ttccgccacc gttttcatgg catcgtcata
     4081 ttttttgagt gtataggcgc tattttttcc gcaggcaata atttccactt ccgggcgcgt
     4141 cagcaaatgt tccacgacag tagcaaacgc atccttcgac agctcgccat taaaaacatc
     4201 tttgccttca ctcattaccc agccgccgtt ttcagccaca aaggcaattt cattcgcaat
     4261 ttcagggaag aaagagatca actgataata ttgattcccg ctggcgacca caaagcgaat
     4321 gccttgtgct ttcatttgct gatactgagc cataaaccgc tcacggttat aggttttttg
     4381 atcgcttaag aaagtaccat ccatgtctac cgcaattaat ttaatgctca tcaactattc
     4441 tccatcgccg tctgcgtttt ggtatcgggt ttggcgacgg ctttcgcgac aatagccgcg
     4501 agaataacca gcgccagtac aaccagcatt gcactacgta atccataatg ttcgccgaga
     4561 tagcccagca gcggcggccc gacgaggaaa gccagataac cggtcgttgc taccacactg
     4621 acgcgggttg gtgcatcggg gccggtatcg ctggcggcag aaatggtcag cgggaagccc
     4681 agcgaggcac ccagtcccca aagtacaaca gacactcctg cgacccaggc gctatcgaca
     4741 aaaataatca gcccaatacc caacgccccc attagtgcac tggcccgaac cacggcaacg
     4801 cgactgtaac tgtcgatgaa ccaaccgccg gtgaagcgtc caacggtcat ccccagggta
     4861 aaaccggcat aaatcagtga gccggaagtg gggctaaaac cgtgaccatc aaccattaat
     4921 aagggtaacc agtcgttggc agaaccttcg gcaaaggcca tcgccagcac tacaacacca
     4981 atcaacagca actggatatc gcgataaaaa ggaatgcctt tttcgccatg ctgggtgcca
     5041 tcggcagcat ttttgcccgt accgtcaggg attgcctgaa tggcgatata aataggcgcg
     5101 atacctacca gcgccgccaa taaaatgtgc accgttgccg gaacgccaaa ggccgtcagt
     5161 gccatcccga caccagcgcc tgccagcgtg cccaggctat aaaaaccgtg catcatcggc
     5221 aaaaccgttt tattcatttc tcgctcaacg gcggcacctt caacgtttat cgccacttcc
     5281 gcagaaccaa aacttgcccc aaagacgccg agaccaacgg caaagagcag gggcgatgtc
     5341 agccagagtg ccagacttaa tatcatcatc ccgatcaatg cgcaggacat cgtgaccagg
     5401 atgacattac gtgtcccaaa gcgtttcact aaccacgccg agcagagaat accgctcatc
     5461 gaaccgatcg acagaccaaa gagaacaccg cccatttcag cgatcgagac agagagaata
     5521 tcgcggatag caggcgtacg ggttgcccag gacgccatta acaggcctgg caaaaagaag
     5581 aacataaaca gcgcccaggt tcggcgtttc aatgcattac gtgaagaatt tacggtcata
     5641 gatcacgtca aaataagaag agggaagaca acattagcaa ggttgtgtac atttgtacac
     5701 aattgcagac agaggaaatg acatgcgtcg cgctaacgat ccgcaacggc gagaaaaaat
     5761 tatccaggcc acgctggagg cggtgaaact ttacggaata catgctgtta cgcaccgcaa
     5821 aattgctacc cttgccgggg taccgttggg atcgatgacc tactattttt caggaattga
     5881 tgagttgttg ctggaggcgt tcagcagttt tactgagatt atgtcccggc aatatcaggc
     5941 attttttagc gatgttagtg atgctcaagg cgcatgccag gctatcaccg atatgatcta
     6001 cagctcacag gttgcaacgc cggataatat ggagctgatg taccagctct acgcgctggc
     6061 tagccgaaaa ccgctattaa aaacggtaat gcaaaactgg atgcagcgca gtcagcaaac
     6121 gctcgaacaa tggtttgaac ccggaaccgc ccgcgcgctt gatgcgttta ttgaggggat
     6181 gacgctgcat tttgtcaccg accgtaagcc gctatcgcgc gaggagattt tgaggatggt
     6241 tgagagggtt gcagggtagt agataaattt tagataacaa aaaacccatc aaccttgaac
     6301 cgaaatggcg gggttgatgg gctccacaaa atggggacat caaagaaaag cagtggcact
     6361 aattaagact gatgccctgc ggaaaagttc tgcggttgtg caaaaaaatt tcattttcag
     6421 ggcaacttca gttttatcct aatcctggcc ataccatgac gatgattgtc cctgccagcg
     6481 tcagcaggac gttggcgatt gcgtaggtgc ccgcatagcc cagcgcaggg atgttactgc
     6541 gagctgtatc actgatgatc tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc
     6601 cgaacaacag tgcgcggttc attcgcaata cataagcacc gaacaagaaa cagataacca
     6661 cgggcacaag actgacaatt aatccggcaa tcaacatctg accgccaatc gcgcccaggc
     6721 cgttattaat accgctaccg gcgctcagac caacgcctgc cataaacacc atcaagccga
     6781 actctttcac catgcttaat gcaccttgcg gaatgtaacc gaaggtcggg tggttagcac
     6841 gcataaagcc cagcataatt ccggcgaata acaacccggc agcgttcccc atgccgaaac
     6901 tgaatgtgct gaactggaag gtgatcatcc cgatcatcag cccaataaca aagaaggcgc
     6961 aaaatgccag caggtcagtg acctggctgt gaatcgagat aaagccgatg cgatcggcga
     7021 tggtttttac gcgacgggca tcaccgctga cttgtaaaac gtcacctttg ttaagcacga
     7081 cgttgtcatc tatcggcatc tcaatctggc tacgaatgac gcggttaagg aagcaaccgt
     7141 gatcggtcaa cttcagttgt gcgagacgtt tacctacagc gttatggttt ttaacgacca
     7201 cttcttcagt gacgatacgc atgtcgagaa ggtcacgatc gaaaacttct ttaccgttac
     7261 ggaagctggg atcgagtcgg gcatgggcgt cgggatagcc taccaacgct atttcatcgc
     7321 ccatttgtag cacggcatca ccgtctggat ttgccagaat cccgttacgt cgaatacgtt
     7381 caatgtagca gccggtttgt cgataaatac ccagttcacg cagatttttg ccgtcggtcc
     7441 aggccaccag ctccgggccg acgcgatagg cgcggatcac cggtaaataa accttacggt
     7501 tggcatcagt gtccaggcca cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct
     7561 gatgctgcaa tttcggcaag taacgcgcac caacaatcaa actcaccaga ccgattaaat
     7621 aggttaaggc atacccgagg ctcagattat ccagtgccag tgagagctgc ctgctttcca
     7681 tgccggaatg atgcagtgta tcgccagcac cgaccagaac cggtgtcgac gtcatagagc
     7741 ctgctaacat accggccgtc aggccaatat cccagccaaa cagcttacct aaccctaagg
     7801 cgatcaccag cgcactgcca accatcacca gtgctaacat taggtaattt ttcccatcgc
     7861 gaaaaaaaat ggaaaaaaag ttcggtccgg cttcgacccc gacgcagaaa ataaacagca
     7921 taaagccaag attaagcgca tcggtgttaa tgctgaaatg ttgttggcct aataacagcg
     7981 atacgactaa aacgccaatg gaattaccca gttggatcga accaagtcgt aactttccga
     8041 gacatagccc aagcgcgagg accacaaata ataacagaat gtaattccca tttaacaatt
     8101 cggcgacgtt tatattcacg gaggctaact tcttgtttac tagtaagctg ttgaaagaaa
     8161 tgctaattta cgataatgtg ttttaccaga attcagggcg cagattcatt cagcgcacct
     8221 aaacgatagt aaagtaacaa tatattttac tagtgtaatc acattaggta tcaacggcta
     8281 tatgaattgc gttggcctat attagcatgg aatgcgaagc ggctttatct tactgaacgc
     8341 cacactggcg aaaaatgtgt tcgatagacg cagtgtcagg aggaacgagt gaaacataaa
     8401 caacgttggg cgggggcaat ctgctgtttt gtcctcttca ttgtggtgtg cctttttctg
     8461 gcgacgcaca tgaaaggcgc ttttcgggct gccgggcatc ctgaaatcgg cttgctgttt
     8521 ttcattcttc ctggagcagt tgccagtttc ttttcacagc gtagagaagt cctgaaacct
     8581 ctatttggcg caatgctggc ggcaccctgt tcaatgctca ttatgcggct gtttttttca
     8641 ccgacgcgct cattctggca agagctggca tggttactaa gcgcggtgtt ctggtgtgcg
     8701 ctgggggcac tgtgtttctt atttatcagt agtttgttta aaccacagca cagaaaaaat
     8761 cagtaaagcc ctcaacgcga gggcttgtca gacgatcagg cgtccagatt ttctttcacc
     8821 catgcagcaa aatcggtata gccgccgata tgttgctgat cgacaaaaat ctgcggcacg
     8881 gtttctacgg gtttacctgc cttttgttgt agatcttctt tagtgatccc ttccgcacga
     8941 atatctacat actgatactg aaaatcatcg cgttcattgc tcaatttctc agccagatct
     9001 tttgcacgca cacagtaagg gcaacccgaa cgaccaaaaa taacggtttg cattatttct
     9061 ctcctcatag atttatgcct gtaatgatca cgctaaaatg tattcgctga aagtaggttt
     9121 aacctgttgc attaattgct aaaagctata actgttaaac acaatacagt gaaaagtttt
     9181 agactgaagg ctcactttgc agagggaagc gtatgcgcgc gatcggtaaa ttgcctaaag
     9241 gcgtgttgat actggaattt atcggaatga tgctactggc ggtggcgctg ctgtcggtaa
     9301 gcgactccct gtcgctgcct gagccatttt ctcggccaga agtgcagatt ctgatgattt
     9361 ttctcggtgt tttgctcatg cttcccgctg cggtggtggt tattcttcag gtggcaaaac
     9421 gtcttgcccc acagctgatg aactgtccac cgcaatattc acgttcagaa agagaaaaag
     9481 ataatgacgc caaccattga acttatttgt ggccatcgcg ccattcgcca tttcactgat
     9541 gaacccattt ccgaagcgca gtgtgaggcg attattaaca gcgcccgtgc gacgtccagt
     9601 tccagttttt tgcagtgtag tagcattatt cgcattaccg acaaagcatt acgtgaagaa
     9661 ctggtgacgc tgaccggcgg gcaaaaacac gtagcgcaag cggcggagtt ctgggtgttc
     9721 tgtgccgact ttaaccgcca tttacagatc tgtccggatg ctcagctcgg cctggcggaa
     9781 caactgttgc tcggtgtcgt tgatacggca atgatggcgc agaatgcatt aaccgcagcg
     9841 gaatcgctgg gattgggcgg ggtatatatc ggcggcctgc gcaataatat tgaagcggtg
     9901 acgaaactgc tgaaattacc gcagcatgtt ctgccgctgt ttgggctgtg ccttggctgg
     9961 cctgcggata atccggatct caagccgcgt ttaccggcct ccattttggt gcatgaaaac
    10021 agctatcaac cgctggataa agacgcactg gcgcagtatg acgagcaact ggcggaatat
    10081 tacctcaccc gtggcagcaa taatcgctgg gatacctgga gcgatcatat ccgccgaaca
    10141 atcattaaag aaagccgccc atttattctg gattatttgc acaaacaggg ttgggcgacg
    10201 cgctaaaacc gccacgtcga tgtatgatac gcgggctttt gaccaggtct gacagagagg
    10261 tgcagggtga aaattgccat attgtcccgg gatggaacgc tctattcgtg taagcggctg
    10321 cgtgaagccg ctatacagcg cggtcacctg gttgaaattc ttgatccgct ttcttgctac
    10381 atgaacataa atcctgcggc gtcttctatt tactacaaag gccgcaagtt accccatttt
    10441 gacgcagtga tcccgcgtat tggcaccgcc attacctttt atgggacggc ggcactgcgc
    10501 cagttcgaga tgctggggag ctatccgctc aatgagtcgg tcgccattgc ccgggcgcgt
    10561 gacaaattgc gttccatgca actgctggcg cgtcagggca tcgacctgcc tgtcacgggc
    10621 attgcgcatt cgccggatga taccagcgat ttaatcgaca tggtcggtgg tgcgccgctg
    10681 gtggtcaagt tggttgaagg cacgcaggga attggcgtcg tgctggcgga gactcgtcag
    10741 gcggcggaaa gcgtgattga cgctttccgc ggtctgaacg cgcatattct ggtgcaggaa
    10801 tatatcaaag aggcgcaagg gtgcgatatc cgctgtctgg ttgttggtga tgaagtggtc
    10861 gctgcgattg aacggcgggc gaaagagggc tattttcgtt ccaatttgca tcgtggcggc
    10921 gcggcaagcg tcgccagtat cacaccacag gagcgtgaaa tcgcgataaa agccgcgcga
    10981 acgatggcgc tggacgttgc tggtgtggat attctgcgtg ctaatcgcgg gccgttggtg
    11041 atggaagtga atgcgtcgcc ggggctggaa ggaatagaaa aaaccaccgg tatcgatatc
    11101 gcgggtaaaa tgatccgctg gatcgaacgc cacgctacga cagaatattg cctgaaaacg
    11161 ggtggttagt cgcaatcaca ttactgatca tggttttgcc tgcgcttttt gcgtaagctg
    11221 tgccggtctt tttatcgaaa gaggttgtac aaaattatga catcgctggt cgttcctggt
    11281 ctggatacgc tgcgtcaatg gctcgatgac ctggggatga gtttttttga atgtgataac
    11341 tgtcaggctc tgcatctgcc ccatatgcag aatttcgacg gtgtctttga tgccaaaatc
    11401 gatctgatcg ataacacgat cctgttttct gccatggcgg aagtccgacc ttcagccgta
    11461 ttgccgctgg cggcggattt atctgccatc aatgccagtt cgctgaccgt gaaagcattt
    11521 cttgatatgc aggatgataa tctgccaaag ctggtggttt gccagtcttt atccgttatg
    11581 cagggcgtaa cctatgagca gtttgcatgg ttcgtgcgtc agagcgaaga gcagatttcg
    11641 atggtcattc ttgaagctaa tgcccatcaa ctgctgttac cgactgacga tgaagggcaa
    11701 aacaacgtta ccgaaaacta ttttctccac tgataattcc tttcgagcac gcagtcgctg
    11761 ccgcagtgac tgcgcgctgc aaaattatct gctgttttta accttttctt aaagattatt
    11821 tcacttctct tgtgtcgatt tggctttatc acatagagca aatacgcata aaaatttgtt
    11881 aaataccgtt ttttaatccg agctatagtc tcaaaccctg gctaaagtta ttcttgcgat
    11941 gcttctatat agcgagcagt gctggccggg agagagttct cttttcttac accgcgccga
    12001 taaaaaaata tgcacgttta ttgcatatct ttcagtgtga caacttttgt tcgtttgtta
    12061 acgaactttc agaaggaaag agatgtgacc gccttaaata aaaaatggct atcgggtctg
    12121 gttgcgggtg ctctgatggc cgtctctgtc ggcacgctcg cggctgaaca aaaaacactc
    12181 cacatttata actggtctga ttatatcgcc cctgacacgg tggccaattt tgaaaaagaa
    12241 accggtatta aagtcgtcta cgatgttttc gactctaacg aagtactgga aggcaaatta
    12301 atggcgggta gcaccggctt tgacttggtg gttccatctg ccagctttct agaacgccag
    12361 ttgactgcgg gagttttcca gccgctggac aaaagcaaat tgccggagtg gaagaatctc
    12421 gacccggagc tgctgaagct ggtcgccaaa cacgatcccg acaataaatt tgctatgccc
    12481 tatatgtggg cgacgaccgg gattggctat aacgttgata aagttaaagc tgtgctgggc
    12541 gaaaacgcgc ccgtcgatag ctgggacttg atcctcaaac ctgaaaatct ggaaaaactg
    12601 aaaagctgcg gtgtctcttt cctggatgcg ccagaagaag tttttgctac cgtgttgaat
    12661 tatctcggca aagatcccaa cagcactaaa gcggatgatt acaccggacc ggcaaccgat
    12721 ctgctgttaa agctgcgccc gaacattcgt tatttccatt catctcaata cattaacgac
    12781 ctggcaaacg gcgatatttg cgtcgctatc ggctgggcag gtgatgtctg gcaggcgtca
    12841 aaccgcgcga aggaagcgaa gaatggagtg aatgtctcgt tctcgattcc aaaagaaggg
    12901 gcgatggcgt tctttgatgt attcgccatg ccagcggatg ccaaaaacaa agacgaagcc
    12961 tatcagttcc tgaattacct gctgcgcccg gatgtagtag cgcatatttc cgaccatgtg
    13021 ttctatgtca acgccaataa agcagccacg ccgctggtga gtgcggaagt ccatgagaac
    13081 ccgggtattt atccgcctgc ggatgttcgt gcgaagctgt tcactctgaa agtgcaggac
    13141 ccgaaaatcg accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg aaaataatcc
    13201 gcagccttag atgccgggcg ggcgcaccac acccgccggc aattcgcacc atcatggtgc
    13261 gcttgcacac attcaatgcc ggagagcagc cgtgaatgac gctatccctc gcccgcaggc
    13321 gaaaacccgt aaggcgctga cgccgctatt agaaatccgc aacctcacca aatcctacga
    13381 tggtcaacat gcggtggatg atgtcagcct gaccatctac aaaggtgaaa tcttcgcgct
    13441 gctgggcgca tccggctgtg gcaagtccac gctgctgcgt atgctggcag gtttcgaaca
    13501 accttctgcc ggacagataa tgcttgatgg cgtcgatttg tcacaggttc cgccttacct
    13561 gcgccccatc aatatgatgt ttcagtctta cgcgctgttt ccgcatatga ccgtggaaca
    13621 gaacatcgct tttggcctga aacaggacaa actaccgaaa gcggaaattt ccagtcgggt
    13681 caatgagatg ctcggcctgg tgcacatgca ggagttcgcc aaacgtaaac cgcatcagct
    13741 ttctggtggt cagcgacagc gtgtggccct ggcccgaagc cttgccaagc gcccgaaact
    13801 attactgctc gacgagccga tgggcgcgct ggataaaaag ctgcgtgacc ggatgcagct
    13861 tgaagtggtg gatattctgg agcgcgtcgg tgtgacttgt gtgatggtca cccacgatca
    13921 ggaagaggcg atgaccatgg cggggcgcat cgccattatg aatcgtggga aatttgtcca
    13981 gattggcgaa ccggaagaga tctacgagca tccgactacc cgctatagcg ccgaatttat
    14041 tggctcggta aacgtctttg aaggcgtact caaagagcgt caggaagatg gcttggtgct
    14101 tgattcgccg gggctggtgc atccactgaa agtcgacgcg gatgcctcgg tggtcgataa
    14161 cgtgccggta catgtggcgc tgcgcccgga aaaaatcatg ctttgcgaag agccgcccgc
    14221 caatggttgt aacttcgcgg tgggggaggt gatacacatt gcctatctcg gcgatctttc
    14281 ggtgtatcac gttcgtctga aaagtgggca gatgattagt gcccagctac aaaacgccca
    14341 tcgtcatcgt aaagggttac cgacctgggg cgacgaagtg cgtttgtgct gggaagtgga
    14401 cagctgtgtg gtgctgacgg tttaaggagc aaagatgaat acacttgaac ctgctgccca
    14461 gtcgaaaccg ccgggcggat ttaagctgtg gttgtcgcag ctgcaaatga agcatgggcg
    14521 caaactggtc attgcgttgc catatatctg gttgatcttg ctgtttctgc tgccatttct
    14581 gattgtcttt aaaataagcc tggcggagat ggcgcgcgct attccacctt ataccgaact
    14641 gatggagtgg gctgacgggc aactgtccat cactcttaat ctcggtaatt ttctgcaact
    14701 gaccgacgat ccgctctatt tcgatgctta tctccagtcg ctacaggtgg ctgcgatttc
    14761 gacattttgc tgtttactga tcggctatcc gctggcgtgg gcggtggcgc acagtaagcc
    14821 ttcgacccgt aatattttat tactactggt gatcctgccg tcgtggacct cgtttctgat
    14881 ccgcgtttat gcttggatgg gaatattaaa aaacaacggt gtgctgaata attttctgct
    14941 gtggctgggg gttatcgatc aaccgctgac cattcttcat accaatctgg cagtttatat
    15001 tggcattgtt tacgcctacg tgccgtttat ggtgctgccg atttataccg cgttgattcg
    15061 tattgattat tcgctggtgg aagcagcgct ggatctcggt gcacgaccgc tgaaaacgtt
    15121 ctttactgtg atcgtgccgc tgactaaagg tgggattatt gccggatcga tgctggtgtt
    15181 tatcccggct gtgggcgagt ttgtgatccc ggaactgctc ggtggcccgg acagcatcat
    15241 gatcgggcgc gtgctatggc aagagttttt caataaccgc gactggccgg tggcctcggc
    15301 ggtagcgatc atcatgttgc tgctgctaat tgtgccgata atgtggttcc acaaacacca
    15361 gcaaaaaagc gtgggagaac acggatgaat aatttaccgg tagttcgttc gccctggcgg
    15421 attgtgattt tgctgctggg ctttaccttt ctctacgcgc caatgctgat gctggttatc
    15481 tattcgttta acagctcgaa gctggtgacg gtgtggggcg gctggtcaac gcgctggtat
    15541 ggtgagttat tgcgcgatga cgcgatgatg agtgcggttg gttcaagcct gacaattgct
    15601 gcctgtgcgg caacggcggc ggcgatcctc gggactattg cggcggtggt gctggtgcgc
    15661 tttggcaggt ttcgcggatc aaatggcttt gcctttatga tcaccgcgcc gctagtaatg
    15721 ccagatgtca tcacgggctt gtcgctgttg ttattattcg tcgcgcttgc tcatgccatt
    15781 ggctggcctg cggaccgcgg tatgcttacc atctggctgg cgcatgtcac tttctgtacg
    15841 gcttatgtgg cggtcgttat ttcgtcgcgt ctgcgggaac tggatcgctc gatagaagaa
    15901 gccgcgatgg atctcggtgc gacgccgctg aaagtgtttt tcgtcattac gctaccgatg
    15961 atcatgcccg cgatcatttc tggctggtta ctggctttta ctttgtcgct tgatgatctg
    16021 gtgatcgcca gctttgtttc cgggccggga gccaccacgt taccgatgct ggtcttttcc
    16081 agcgtgcgga tgggggtgaa tccggaaatc aacgccctgg caacattgat cctcggcgcg
    16141 gtcggaattg tcggatttat cgcctggtat ctgatggcgc gcgcagaaaa acagcggata
    16201 cgcgacatcc aacgtgcaag acgtggctga agacactaaa atttgccaac ctggctacat
    16261 aatgccgcgc atgtcgcggc attgttttca tggaagacga aacgttggga ttttttaaga
    16321 aaacatcttc atctcatgct cgcctgaatg tgcctgcgct ggtgcaggtg gcggcgctcg
    16381 ccattattat gatccgtggc ctcgacgtgc tgatgatttt caatacgctg ggcgtgcgcg
    16441 gtattggcga gttcattcat cgcagcgtac aaacctggag tttaacgctg gtctttttaa
    16501 gcagtctggt gctggttttc attgagatct ggtgtgcgtt ttcactggtg aaagggcgtc
    16561 gctgggcgcg ctggctatat ctgctgacac aaatcaccgc cgcaagttac ttgtgggcgg
    16621 cttcgctggg gtacggttat ccggagctgt tcagcattcc cggtgaatca aaacgtgaaa
    16681 tcttccatag cctgatgctg cagaagctgc cggatatgct catcctgatg ctgctgttcg
    16741 ttccctcgac cagtcggcgg ttcttccagt tgcaataatg tgtataatcg tcgcccctga
    16801 tgatgtgaag gtcaatgtat gcagtgcgca ctttacgacg cgggtcgctg tcgttcctgt
    16861 cagtggataa cgcagccgat tccagagcaa ctctccgcta aaaccgccga tcttaaaaat
    16921 ctgctcgccg attttccggt tgaggaatgg tgcgcgccgg tgtcaggccc ggaacaaggg
    16981 tttcgtaata aagccaaaat ggtggtgagt ggtagcgttg aaaaaacact gctcggtatg
    17041 ctgcatcgcg atggcacgcc ggaagacctt tgtgactgcc cgctttatcc agcctcattt
    17101 gcgcccgttt ttgcggcgct aaaaccgttt atcgcccgag cggggttaac gccctacaac
    17161 gtggcgcgta agcgtggcga actgaaatac attctgctga ctgaaagcca gagtgatggc
    17221 ggcatgatgc tgcgctttgt actgcgttct gataccaagc tggcgcaact gcgtaaggcg
    17281 ctgccgtggt tacacgaaca actaccgcag ctgaaagtta ttaccgtcaa tattcagccg
    17341 gtacatatgg cgattatgga aggggagacg gagatctacc tgaccgaaca acaggcactg
    17401 gcggagcgtt ttaatgacgt accgctgtgg atccgtccgc aaagtttctt ccagactaat
    17461 ccggcggtcg ccagccagtt gtacgccacc gcgcgcgact gggtacgaca gctgccggtt
    17521 aaacatatgt gggatctgtt ctgcggtgtg gggggctttg gtttacactg cgcgacgcct
    17581 gacatgcagt taaccgggat cgaaattgcg ccagaggcca ttgcctgtgc aaagcagtca
    17641 gccgctgaac tgggcttaac tcgtttacaa tttcaggcgc tggactccac tcagtttgcc
    17701 actgctcagg gggaggtgcc ggagctggtg ctggttaacc cgccgcgccg cggcattggt
    17761 aaaccgctgt gtgattatct ttcaacgatg gcaccgcgtt ttattatcta ctccagctgt
    17821 aacgcccaaa ccatggcgaa agatatccgc gaactgccag gttaccgcat cgaacgggta
    17881 cagctttttg atatgttccc gcacaccgcg cactatgaag tgttgacgct gctggtgaag
    17941 caataaaaaa gccacaggtg cggcttcaga ttgctgatca ggtgcgcttt gttcatgccg
    18001 gatgcggcgt gaacgcctta tccggcctac aaaatcatgc taaatcaata cattgcagag
    18061 ttgatgtggg ccggataagc gcagcgcatc aggcaatctt acttttgtca tcagtctcaa
    18121 ttactgcgga aaccactggt cattgatttt ctgatatgta ccgtcagctt taattgctgc
    18181 cagcgcgtta ttcagttttt ccagcagagc tttgttatcc ggacgtacag cgatacccag
    18241 gccggtgccg aagtattgcg gatcggtcac tttttcagtc gcaacgccca gttgcggatt
    18301 ggttttcagc cattcgttta ccaccgcggt gtcaccaaat accccatcaa tacgaccatt
    18361 tttcagatcg ataaaggcat tctgataact gtcataggag acagttttca cttccgggtg
    18421 ctgatcctga atatatttct ggtgcgtcgt accgttttcc atcccaatgc gtttgccttt
    18481 caggtcggca aacgttttgt aggtatcttt tttggaaatc acgacggctg agttttcata
    18541 gtagggtgtg gtaaacgaaa cctgcttgct acgctccggg gtgatatcca taccggagat
    18601 tacggcgtca tattttctga atttcagtga cgggatcagg ctgtcgaacg cgtgattagt
    18661 aaaagtacat tctgcctgca tttgtttgca caaggctttt gccagatcga tatcaaagcc
    18721 gacaatctca ttattagcac ctatagattc aaagggtgga taggtggctg aaacgccaaa
    18781 attgattttc tctgcggcag aagcaccgaa agtaaaggaa gcaagtaaag cggcaagaac
    18841 taactttttc atgatggaac tcccgtctgt caatcttatg atttttggcc gtgtctgcgg
    18901 catgggataa caatgccatc aagtgaattt atatgcaata aacatgatta aataatttaa
    18961 atgaaataaa aaaagacgga taacttagtg agttgtccgt cttcattata agaatttatg
    19021 cactatgtag gccggataag gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc
    19081 tgatgcgacg ctggcgcgtc ttatcagacc tacaaacccc cccggcgaat gtacgcagct
    19141 acattaattc cgccgttcga atgccagtgc tttgcgctcg atcagacgca tcatcagcgt
    19201 cagcaggccg ttgacgacca agtaaataat ccctgccgca ccgaacacca ttacatcgta
    19261 ggtgcgtccg tacaacaact ggctgtatcc catcacttcc atcagcgtaa tggtgtaagc
    19321 cagagaggta cttttgaaca ccagcaccac ttcgttggaa taagaagaga gcgagcgttt
    19381 aaaggcatac ggcagcagga tcgccagcgt atcttttttg ctcattccca gggcgctaca
    19441 ggactgccac tgaccttctg ggatcgcacg aattgcaccg taaaacagct gcgtggtata
    19501 cgccgcactg ttcagcgata acgcaatcag cgcacataac cacggttctg acaacaaatg
    19561 ccacagtgcc ggatactcct gcaacgtcgg aaactggccc ggcccgtaat aaatcaggaa
    19621 gatctgcacc agcagcggcg taccggtaaa cagcgtgata taaccccgca ccagccacac
    19681 cagcaccggc gttttcagcg tcaggatgat ggtaaaaatc aatgccagaa tcagtgccac
    19741 aatcagcgag gcaacggtta gcgtcaggct ggtgtgcagc cctttcatca gctcgggtaa
    19801 atactcaaac attagctggg cctccgctca aaacgtgtcg cgcgcaggtc aatgcgtttg
    19861 agaatgtact gactgagcag ggtgatcacc aggtaaatcg ccgccgccac aatgtaccag
    19921 gtaaatggtt cctgggtacg agtagcaatg cttttggttt gtaacattaa atcattcaca
    19981 ctaatcaaac tgaccagcgc ggtatctttc agcagcacca gccactggtt accgaggcca
    20041 ggcagcgcat gacgccacat ctgcggcatc accagacgga aaaagatagc cgatttcgac
    20101 agccccagcg cctgaccaga ttcccactgg cccaccggta cagctttcaa cgcgccgcgc
    20161 agcgtttgcg aggcataggc ggcatacagc agtgacagag cgatgacacc acaaaggaac
    20221 ggactcacgt cgaagttctc aatgtccatc tgcaccggga tctgcacgaa cccaagattg
    20281 atagtgaagc catccgaaag cgtcagcagt aactgcgagg agccaaaata gataaacagc
    20341 actaccagaa tttctggcag gccacgcaga atggttacca gcgctgaacc tgcccacgcg
    20401 acaggacgcc atttcgctga ctcccatacc gcaaagaaca tcgccagcgc cagcccgaca
    20461 atcaatgcac aaacggcaag gccgacggtc atcccggcgg cgcttgctaa aggaaaaaat
    20521 tcattcatca ggaattactt ctggaaccat ttgttgtaga tggtttcgta agtgccatct
    20581 ttcttcactt tttccagcgc agtgttgagt ttctgctgca gttcagtgtt tccctgacgt
    20641 accgcgatgc cgaggccagt accgaaataa tctttatcgg tcactttatc gcccactgcc
    20701 gccagttttg ggttatcttt cagccactca gtgaccactg cggtgtcacc aaatacgctg
    20761 tcgatacgcc cgttttgcag atccagtttt gcgttctggt agctgtcata cggaacggtg
    20821 gtgatttccg ggtgcttatc cataatgaac ttctggtgtg tcgtcccgtt ctgaacgccg
    20881 acttttttgc ctttcagctg atcaacactg gtgtatttgc cttgctgacc cacaaacagg
    20941 gcagagttgt catagtacgg ggtagtaaac agtacctgct tttcacgctc cggagtgata
    21001 tccatgcccg ccatcactgc ttctacgcga cggaatttca ggcttgggat caggctgtca
    21061 aacgcctggt tagagaaagt acaggttgca tcaatctctt tacacagtgc ctgcgccagg
    21121 tcgacgtcaa aaccaacgat ctggttgttt gcatcaatcg attcaaacgg aggatagggg
    21181 gcttcggtag caaaacgaat ggtttcggca gctgtggcgg aaagactaaa acctgcaatt
    21241 aacgcggcaa tcagaacttt tttcattgtt gttatcccga atcttagtga gagagatagt
    21301 ttttaaatgc ttcggtttgc ggctcggtaa agcagctcgc gtcgccttgt tctacgatat
    21361 gaccattttc catatacacc actcggctgg cggttttacg cgccacttca acttcgtggg
    21421 tgacgatcac ctgggtaata ttcgtttctg ccagctcacg aatgatgctg acgatttgtg
    21481 ccgtaatttc cgggtccagt gcggcggtcg gttcatcgaa cagcagtacc tgcggttcca
    21541 tcatcaacgc acgggcaata gcaacacgct gctgctgacc accagaaaga tgcagcgggt
    21601 aacgatcgct ataaggtttg agacgcagac gttccagcag tttttctgca cgggccagcg
    21661 cctgatcttt actcaacccc agtacacggc agggcgcttc aatcaggttt tgctgcacgg
    21721 tcagatgcgg ccacaggttg tattgctgaa acaccatgcc aacgttacga cgcaaatcgc
    21781 gaatcgcttt gtcagagggt gttttggtga aatcgaaatg gttgcctgca atgttgagcg
    21841 taccggagcg cggcatctca agcagattga gtacacgcag cagcgagctt ttacccgcgc
    21901 cgctggggcc aagtaacacc agcgtttcgc cctgtgggca atccagcgtg atatcgaaca
    21961 gcgcctgatg cgcgccgtag aagcaattaa tgccgtttaa ttgaatactc attgacactc
    22021 gtatactggc agtctgatag ctattgaggt cgaagatagt acctttaaca gaataattat
    22081 gcaatattta tgctttaaaa gttaaaagca aagcgcatta ttcaataaac atagcacaaa
    22141 ataacggggg cggtggtcgg tgagcataaa tgtcggcatt cctcacgaaa tgccggataa
    22201 tttacggggt ttattggttg atcaaggcgt tagcgattct cgatggactg acggagcgta
    22261 cccgccgtgg catgaacgct accgcctaag tagcgcacat cgtcgatgac ccaacactgg
    22321 ccttcctgaa tcattaacac ttcatcctgc cagccctggt caccctgttt gagatccacc
    22381 cgcaatggaa tgttacgggc atcacgatta gggatagtcg atgcactggc aacgtgggcg
    22441 ctgtctggca aggtggttcg actggagaac ggatcgctgc ttaacaattc acgatgacta
    22501 ttatcgcggc tggcatcgct aagttgggtt gccagtttgt cactcaaata ggggcgcagg
    22561 gcggtaatgt cattgctgcg atgttgaatg cggtagtcgt agaattgttg agccacactg
    22621 tcaggccctc cctggacaca ggaaccggtg cgggggccgt tatctttata agctggcgtg
    22681 acggtggtac aggcactcag cagcagtgca catggaataa gtagagatag tgaacggtag
    22741 cgcataatca tttccttatt agcttgctgt ctgcgtcaca ctacgaccaa acgctatcag
    22801 attatttaat acattgtttt taatacaatt ctgaaacaat ctgaacacct gattgggttt
    22861 gagtccattg ctggcgaccc actcaaggtt gctcgtagct gacttactta aattacccaa
    22921 acaatagaca gtgtaaacca tttgtgaggt tgagcgtgtt atatggctga aatgatcatt
    22981 taaggtaaat atgatagttc tgaaatggtg gtataaatcc tcctgtagag gttaagagca
    23041 tctgcaagaa gccagtttca catttataac gcgtgataaa tgaatgcggt tattgtctaa
    23101 tcattacaaa atttttcaca tgcgataaga gatcctgcga ttaagatgat tgccaaaatt
    23161 acgttgtatt tttgaaaatg agcgaaattt gatagtccca gctttgactc tcgataatat
    23221 tatttatgct acaattgtgg gtaaagaatt taagctaatg aatttgagtg aagaataaat
    23281 taaattgctt cagtatttcg ttaattaata taaatacaaa aggcgttgtg cttttataat
    23341 tataaagcgc gaggagttcg gtggattatc gtttttcttg ggtgattttt aatatgataa
    23401 acaatatatc tgatcaagca tcatcatttc caggcacaca actaaatcaa agtgataatt
    23461 tcctggattc actgagggaa tttttcgcga tattaaatcc cagtcgtaag ggtgagttgt
    23521 ctacatggga taccatctac ttgcatctga tattagcaat aaatgctgac agtgatctca
    23581 taaaaaatga tgtgttgctc gcggaaaata ttccatcagc gaattaccaa tttaatacat
    23641 tttttagcaa tactttcgag attgatgtta aaaaatatct gggtaaatca gaagataacg
    23701 aagttgaaat aaaagcgggt aatgaaagaa tctctattgg aatacgtaat gtatcgaatg
    23761 gaaaacttga aagacaacag tttcttttcc cactggacta tgagaataaa ttacaagaac
    23821 aattagataa gtattttacg attgaatcgc acccattatt atacaggtat accattggta
    23881 gtaagattgc gaatgtgatt tttgaaaagt tatacagtcg tattgacttt aacaaagaac
    23941 aatatatatc ctttataaag gacgcattta ttcattttta tgattatagc aggcgatatg
    24001 ctattagtga gaatatcgat aaagacgctg taacaaacaa tatcgcattg atgagcactt
    24061 tttatgacag tgataatacg tcaggtgagg ttttaaataa tgattttaca gaagaagagt
    24121 catttgaaac tgcgttagat gtagagcatg caatagtgct tggatttgct gatgataatt
    24181 ttgaaacaaa acctgttcat tatcaggatt tattaacacg atttagtgcc tttcaagata
    24241 ccgtgtttaa tctgtttcct gaaatgcact cttcgcatta ccacgatatt tgtagcgtaa
    24301 gtgtggatat gacaaaggga acgcaatgca tgattcactt gatggtgaat gaagaagttt
    24361 tcatgtctct gcctgttcca gttgccacga tggtacgtga agatgcaagt aatttagtca
    24421 atcttaaaac actactcaat gatggttgtt tcattaaata tagtcatttt aatgacgtgg
    24481 cattaataaa gcaaaacata agtaatctct atttatcaca cactgttgtg aatgaaagca
    24541 tattgaaaaa atgttgtttt gaaaacggca gtttaggtga tgttaaaata acaaatagca
    24601 atgtaataaa cagtgcattc aaaaatattt cgtttagatc tgttaaaatt aataacgtaa
    24661 atactcattc tttaaaattt attaactgca attttttcaa tgttgatatg ataagagtta
    24721 atctcagcaa atgtcttttt catgaatgta gtatgcatgg tgtcaagata aaaccctggt
    24781 tgcctgtaaa atggacaaaa gaattaataa gtgattattt gtatggctgt ttactatctc
    24841 tgtactctat ctgtgcaagg gatatatata atatgaacgc cgggaataac gtgaaggttg
    24901 ctgcggatgc tttccttgaa attattttct ctcttaaaaa taaatattgt attaaactat
    24961 taagcgctca ggatcgggcg tttatatacg agtttgcaag aatgatcttc gcttatatta
    25021 atgacaaaag cattgagatc ttattacttt cttgttttgc ggcggctgac caaaaagcaa
    25081 tccagcggta tagacctcag tcacaagatg gggaagactt taggagtcat ttacaatata
    25141 agttgcctct ttccgctcat taatgttgtt ggtgcaaaat aataagacag gtaagcggta
    25201 ccctataatg aaaccaatat cgggttatgc aacggataat cgtaaagact taagtgggtg
    25261 tttttgacac aatgagatga tttcctaaac tgatcaaaac cttcttccca acatggcata
    25321 tcagtccgat aatgctttag agatcgaagg cttaacaaac aaggagatcg atcatgcaat
    25381 tttctacaac cccaactctg gaaggcctga ccatcgttga atattgcggt gtggtgaccg
    25441 gcgaagcgat tttaggtgcc aatattttcc gtgatttctt tgccggtatc cgcgatatcg
    25501 ttggcggacg ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag atcgcctttg
    25561 aggaattagg ctcccaggcg cgggcgctgg gggccgatgc cgtagttggt attgatatcg
    25621 actacgaaac ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc ggtacggcgg
    25681 tgaaaacgcg tcgatgagaa gagtcttctg gctggtcgct gccgctctgt tactggcagg
    25741 gtgtgcaggc gaaaaaggca ttgtcgagaa agagggatat cagcttgata cccgacacca
    25801 ggcgcaggcg gcgtatccgc gcattaaagt gctggtgatc cactacaccg cagatgattt
    25861 tgatagctcg ctggcgacac tgactgataa gcaggtcagc tcgcattatc tgatccctgc
    25921 ggtgccaccg cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg aacaagaact
    25981 ggcctggcat gcggggatta gcgcctggcg cggggcaacg cgccttaacg acacctctat
    26041 tggcattgag ctggaaaacc gtggctggca aaaatcatcc ggagtgaaat attttgcccc
    26101 gtttgaacag gcacagattc aggcgcttat tccgctggcg aaagatatta ttgcccgtta
    26161 tcacatcaag ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc gcaaagacga
    26221 tccggggcca ttatttccct ggcagcaact ggcgcagcag gggattggtg cctggccgga
    26281 tgcgcagcgg gtgaactttt accttgccgg gcgcgcgccg cacacaccgg tagataccgc
    26341 gtcattgctg gagcttttgg cgcgctacgg ttatgacgta aaacctgata tgacaccgcg
    26401 cgagcagcgg cgcgtgatta tggcattcca gatgcatttc cgcccgacgt tatataacgg
    26461 cgaagcggat gcagaaactc aggcgattgc cgaagcattg ctggagaaat acgggcagga
    26521 ttagcgcggc agttttccgt ggtcgcgtag ccaggcggca gttttctcga tgccttcatc
    26581 cagggtgatg accggctgat aacctaactc ttcctgcgca cgcgtaatat ccagcgtaaa
    26641 gtcaaaattc aacttggagg cgccgtagtg ggtcagcggc ggctcttttg ctgacttgcg
    26701 gcctaaacgc tccatgctgc gggcgatcat atccagcatc gggtaaggga cggaacgaat
    26761 acgacagtca atattcaact cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg
    26821 ctcgccgttg gtgatgttgt acacacgccc ggaaggtagc ttatcgcagg cttcctggct
    26881 tgccagccac attgcgtgca cggcattttc atagtaggtc atatcgacca gcgcactgcc
    26941 gccatgcggt aacagaatac tgccgtagtg gtgcatcata tgcgccagac ggggaataaa
    27001 gactttatcg tgcggtccga acagactttg tgggcgcaga atagtaaagc gcgtttgtgg
    27061 attcgcctgc gaaagcatat tgatcacttc ttcgctggct gctttgctgc gggcaaactc
    27121 gttggcgaag cggtgagggc gaaaatcttc tttaatattg cgatggtggt gataatcgaa
    27181 gtacagggag ggggaagaga tatgaataaa gttacgcaca ccccaggcga cagcccattc
    27241 acccaggcgg cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg
    27301 tgaggtaaag ctggagcagt gccacagcgt atcaatgccc gcgagcatca ctttagcttg
    27361 tgatgaaacc agctcggtca gatccgtcgg aacaaactct gcgcccattt tttccagcaa
    27421 tttacccatt gcttcgttgc gaccgctcgc acgcacgctg atgcctttct ggcataaaaa
    27481 ctctaccgcg tttcgaccta agccgctggt ggcgccggta accagtacct tcatatcaat
    27541 ccactgttgt tgagaaaatg acgtgcgcat tcttccgtga tttcccccat gatgcaatgg
    27601 gaaacatgaa agaataacgc aggttttgtc gattaatctg tgctttgttc tgccagtctg
    27661 gcgatttgtt ttgccattcc gcgaaaaata aacagatgcg cggggatcat caataaccag
    27721 taaaacagcc ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat agtacgatag
    27781 tcacctttat cttccagggt aaaacacagt cgtcccagcc ccggcgcttt catgccaaat
    27841 aacaacgtaa gttgcttttt cggttcaacg acaatcactt tccagctatc caccgcatcg
    27901 ccggtctgta aatattcgcg ctccgggcgg cctttcgcca gattatgacc gatcgcgcgg
    27961 tccatcaacg cccgtgtctg ccacaaaata ttgccaaaga aataacgctc tttaccgccg
    28021 atttggttca ccaccagcca taaagccgca aggctggcgg acgttttaac ggtaaacccc
    28081 gcctgtttgg cgaaataacc gtactccggt cgccagcggg caaaggcctg agcgtcgtag
    28141 ccccagtcgc tggagttgac cagtttttct tcctctttca acgtgctacg taccgcgtca
    28201 tcgaaagcga tcagccgttg tgggatgagt gcacgtagcg cggtatcatc tgccagcaga
    28261 tcgtgtttca gcccctgaat caacgccctg gcggtggtgg gcggtacgga agtaatcaca
    28321 ttgagaaacc acaccgaaat ccagcgggtg gggaggggga tggggatcaa ccagcggcgc
    28381 ttaccgctca ccgccataaa atgttcaaac tgttgctgat aactgagcac ctctggtccg
    28441 gcggcttcga agatgcggtg ttcgttggct ggatggtcta acagcgccac cagatagtgc
    28501 agcaagtttt ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg cggcgttaac
    28561 actggcaggt tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc tgcgccaacg
    28621 ataattccgg cccgaagttc ggtcactggt acattcgctt cacgaagaat gtccgccgta
    28681 gcctgacgag ctcgcagatg atccgactgc tcatgtggcg gggcctgcaa cgaactgaga
    28741 aagattaatt gcttaactgg tacttcacgt agcgcatcgc ggacgttgag agccagctgg
    28801 cgctcctgag cgataaaatc gccgccttcg cccatgctgt gcaccagaaa atagacggta
    28861 tcgatatcct gcaacagggc cggaaggtta tccggccagc tgagatcgac tttatggcaa
    28921 ctgacgtttg ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc cgctaggatc
    28981 tgatgccctt gctggctgag tgtgcgcacc agatgctgac caatgtagcc actggcaccg
    29041 agaactaaaa tgcgttgcgg cacgtctctc tccttaacgc gccaggaatg cgcgccagtg
    29101 ggcggcgact tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca ggcggacaat
    29161 cggcgaggcg ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg cggcagcatt
    29221 ttcttccccg acgcgaacaa acagcatatt agtgtcctga cgcatcacat ccgcgcctgc
    29281 ttcacgcagt tgctccgcca tccaggcggc gttgtcgtga tcttcctgca accgtgcgac
    29341 gttattttcc agggcataca tcccggcggc agccagaatc ccggactgac gcatcccgcc
    29401 accggccatt ttccgccagc ggatggcacg tttaatgtaa tcacgattac cgacgagtaa
    29461 tgaaccgact ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat cacaatattg
    29521 cgtgatctct ttcagttcgc agccgtaagc caccacggca ttaaagatgc gcgcaccgtc
    29581 aacatgcagc gccagattgc gctcgcgggt aaattcccat gcttctttca ggtattcacg
    29641 cggcagcact ttgccgttgt gggtgttttc cagactgagt aatttggtgc gggcgaaatg
    29701 gatatcgtcg ggtttgattt tcatcgccac tttatccagc ggtagcgtgc cgtcggcagc
    29761 cgcgtcgatg ggttgcggct gaatactgcc cagcaccgct gcgccaccgg cttcaaacag
    29821 atagttatgc gcggcctgac cgacaatata ctcttcgcca cgttcgcagt gactgagcag
    29881 agcgaccagg ttggcctgag tgccggtagg cagaaaaatg gcggcttctt taccggaaag
    29941 ctctgctgca tagtcctgca gagcattaac ggtagggtcg tctccgtaaa cgtcgtcccc
    30001 aaccggggcg gccatcatcg cttcgagcat ggcgcggctc ggtcgggtaa cggtatcact
    30061 gcgtaaatca atcatggcat gtccttatta tgacgggaaa tgccaccctt tttaccttag
    30121 ccagttcgtt ttcgccagtt cgatcacttc atcgccgcgt ccgctgataa ttgcgcgcaa
    30181 catatacagg ctaaaacctt tggcctgttc gagtttgatc tgcggtggaa tggctaattc
    30241 ttctttggcg accaccacat ccaccaacac cggaccgtcg atagagaagg cgcgttgcag
    30301 ggcttcatca acttcagagg ctttttctac acggataccc gtaatgccgc acgcttcggc
    30361 aatgcgggca aagttggtgt cgtgcagctc agtaccgtct gtcaggtaac ctccggcctt
    30421 catctccatc gccacaaagc ccagcacgct gttattaaag acgataattt tcaccggcag
    30481 tttcatctgc actactgaga ggaaatcgcc catcaacatg ctaaaaccgc catcgccgca
    30541 catggcgacc acctgacgtt ccggctcggt tgcctgcgca cccaacgcct gcggcatggc
    30601 gttagccatc gaaccgtggt taaacgaacc taacaggcga cgcttgccgt tcattttcag
    30661 ataacgtgcc gcccataccg ttggcgtgcc gacgtcacag gtaaagatgg cgtcatcggc
    30721 ggcaaaatga ctaatttgct gcgccagata ttgcgggtga atggctttct cgctcggttt
    30781 agctaaatcg tccagccctt tgcgggcatc gcggtaatct tccagccctt tgcgggcatc
    30841 gcggtaatct tccagcgctt tatccagaaa cttgcgatcg gttttttctt ccaccagtgg
    30901 cagcaatgcg cgcagggttg atttgatatc gccgaccagc gccatatcta ccttgctatg
    30961 cgcgccgatg ctggctggat taatatcaat ctgaataatt ttggcatcgg tcgggtagaa
    31021 cgcacggtag gggaactgag taccgagtag cactaacgtg tcggcgttca tcatggtatg
    31081 gaaacctgac gagaatccga ttaacccggt cattccaaca tcatacggat tatcgtattc
    31141 gacatgctct ttaccgcgta gggcatgaac aataggtgct ttaattttcc cggcaaactc
    31201 aactaactct ttatgcgccc ccgcgcagcc gctgccacac atcagggcga tattgctgga
    31261 ataacgcagc agttgcgcca gtttgcgtaa ctcttcttct tccggcatca cgactggctg
    31321 tggcgcatga taccagtgcg tggttgcccc ttctggcgca ggttttaacg ccacgtcgcc
    31381 cggtaataca acaacggaaa cgccacggtt aagcaccgct ttgcgcatag caattgccag
    31441 cacttgtggg atctgctccg ggctggaaac cagctcgcaa tagtgactac attcgcggaa
    31501 tagctcttgt gggtgggttt cctggaaata gccgctgcca atttcgctgg agggaatatg
    31561 agcggcaatc gccagtaccg gaacgttatt gcggtggcaa tcgaataggc cgttgatcaa
    31621 gtgcaggttg cctgggccgc acgatccggc acagaccgcc agttctcctg aaagttgtgc
    31681 ttcagcgcca gcggcaaacg ccgccacttc ttcatggcgg gtggacatcc actcgatggt
    31741 gcccatgcga ttaagactgt cgctaagacc gttcagagag tcgcccgtga ctccccagat
    31801 gcgtttcacg cctgccgatt cgagtgtttt ggcgatataa gctgcaaccg tttgtttcat
    31861 ggttctccat ctcctgaatg tgataacggt aacaagttta gttcatctga cggaggggga
    31921 agggatggga gagaaaggag gcactaacgg ttaaatagcc cgatgaaagg aatatcatcg
    31981 ggcataaggc gattatgcga gaaccaaatc cccctgcgga tggcaggagc aggccagtac
    32041 gtaaccttca gcgatttcgg cgtcggtcag cgtcattgta ctgctcaccg tatattcacc
    32101 ggaaaccact tttgtcttac agcagccgca aacacccgca cggcaggcag cgacaaccgg
    32161 aacgttattg ctttccagcg cctccagtag cgtggtgcca accggggcgt aaaattctcg
    32221 tgccggttgc agtttggtga atttcagacc gctggtcgcc gcttctgcta ctggggtgaa
    32281 gaatttctct ttaaagaaac gcgtcacgcc gagcgctttc acttcctgct ctacccaatc
    32341 catatacgga gccgggccgc aggtcatcac ggtacgtgaa gctaagtcag gtacatctgc
    32401 cagcagttcg cgagtgaggc gaccagcgat aaagccttcg gtaacgttat tttctgccac
    32461 cagcgttacc ggatagttac gccactcatc ggcgaaaata acatcctgcg gcgtacgcac
    32521 gttgtagatc acccgcacat cggcctgtgg acggttcttc gcaagccagc gacgcatcga
    32581 cataatcggc gtgacgccgc agcctgccgc cagcaacagg aatttatctt ctgctttatc
    32641 gtcgcaggta aattccccca tcgcgtccga aagccagaga taatcaccgc gttttacatc
    32701 gcgcgtcagc cactgggagc cgacaccgtc atcaatccgc cgcacggtca gggtgatata
    32761 ttcactcacg cctggcgtgg aggaaatggt gtaagcacgc agcgtttccg ctgagtttcg
    32821 cacgctgacc agtgcatatt gcccggcgcg atatgggtag tgatcgtggc aaatcaggga
    32881 aatcgtccac acatccggcg tttcttgcgt aatgtgatga acctgtatcc gccacgggca
    32941 ttgattcgtt ggcatcgtca tcgacaaact ccttacgcgt tcaacagttg cttcatgtct
    33001 tcttcaacag tggtgataga acgcaggccg aatttctcgt tcagcaccgc cagcaggtct
    33061 ggtgtcagga aaccaggtgc agtcgggccg gtgacgatat ttttcacgcc cagagaaagc
    33121 agcgtcagca gaatgacaat cgctttctgt tcaaaccagg agagcaccag cgacagcggc
    33181 agatcgttca caccacagcc cagtttctct gccagagtga cagccagaat aattgctgag
    33241 taagcatcgt tacattgacc tgcatctacc agacgcggca gaccttcgat atcgccaaac
    33301 tcaagtttgt taaagcgata tttaccacag gcgagggtca ggatcaggca gtcatccggc
    33361 acgctggtgg cgaaatcggt gaagtagtgg cgctcgccgc gtgcgccgtc acagccacca
    33421 agcaggaaga tatgacgcag tttttcacgg ctaaccagat caatcagcgt atcggctgca
    33481 ccaagcagcg tctggcgacc aaagcccacg gtgatcaggt gaggaatttc gctgtacggg
    33541 aagcccgcca tttgttgcgc ctgggcgatg accgcagaga aatcttcgcc atccagatga
    33601 cgcacgccag gccagccaac gatgctgcgg gtccagatac gatcgtcata agcgcctacg
    33661 gttgggtcga tgatgcagtt cgaggtcatc acgatggggc cagggaaacg agcgaactcc
    33721 acttgctgat tctgccagcc gctgccgtag ttaccgacca gatgcttgaa tttacgcagc
    33781 tccgggtagc catgcgcagg cagcatttcg ccgtgggtgt agacattaac gcccgtgcct
    33841 tcggtctgtt ccagcaggtt gtagagatct ttgagatcgt gaccggaaat cagaatgcat
    33901 ttacccgccg tcgctttgac gttgacctgg gttggcgtcg ggtgaccgta tttaccggtt
    33961 tcgcctgcat ccagaatgct catcactttg aagttcatct ggccgatttc cattgaacac
    34021 tcaagaagcg cgttcatatc ggcaggccag gtccccagcc acgccatgat tttatggtac
    34081 tgggcataaa tatcgttgtc gtattgaccg agaacatgcg cgtgttccat ataggccgcc
    34141 gcacctttca ggccatacag gcacagcaga cgcaggccga gaatgttttc gccaatcgcc
    34201 gctttatctt tgttaggggt aaattctgct gcctgacgtt gcagctcgcc gagatcatcg
    34261 ctcaccagtt gcaggtcagc catcgggtta tcgacgcgcg cgttggcatc tacagccagg
    34321 cattgtgctt tcagcgcctc gcgcaaggca atcgcttcac gagcgtagcc gacaatacgc
    34381 ggagaatcga agttaacgtt ggtcagggtt gagaaaaagg cacgtggcgc gaagctgtca
    34441 acatcgtggt tgatgatgcc gtattcacgc gcttttaccg cccaggcaga aagcccttgc
    34501 agcgccgcga tgagtaaatc ctgaaggtca gaagtttccg ccgttttacc acacatcccc
    34561 tgcgcgtatg agcagccgtt ttctgccgga gtacggatag tttgttcaca ttgcacacaa
    34621 aacatgatca caccttttaa agttatattt aatatacatg tttaagggta agacgcttaa
    34681 cgcggggata aaagggattt ttcatgcaac tttaagggag attgatttag cgcaattttg
    34741 gcggcaggga actaccgcca gagaggtatt acgcagagaa aaaggcgatg aggatcggca
    34801 ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg gaccatatcc agcccgccag
    34861 tacgttgaag aacgggcagg gtgaaatcca ttgatgtggc accgcataag cccagtgcag
    34921 tagagcggct gcggcgaatc agcccaggga tcaacataat agcaatcagt tcacgggcca
    34981 gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa agattcggtc aataaaatac
    35041 cggaaagaga ataccagccg aaaccggagg ccattgccag cgcggtattg atggggagat
    35101 caagaataaa ggcgttaatt aaaccaccaa ttaatgaact gacaaccacc accacggcga
    35161 caatcattcc ccggcgatta aggacaatct gctttaaggt catgccatta ttgcgcaact
    35221 gaataccaac gaggaaaagt agcaaaatta acgtgtattc actggcttcg gtcgcgtgtt
    35281 gtaagaaagc cagtccactc agaccaatgg caaaaccaat cactactacg ccgcacagtt
    35341 ttagcgactc cagcgccatc gcaatacgcg acgggagttt ttcttgctga tggtggttgc
    35401 gccacggcag gcctcg
//
LOCUS       DAJTYH010000032        35310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTYH010000032 DAJTYH010000000
VERSION     DAJTYH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..1065)
                     /gene="setB"
                     /locus_tag="OO609_001566"
     CDS             complement(<1..1065)
                     /gene="setB"
                     /locus_tag="OO609_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6633032.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWII"
     gene            1433..2563
                     /gene="fruB"
                     /locus_tag="OO609_001567"
     CDS             1433..2563
                     /gene="fruB"
                     /locus_tag="OO609_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6633033.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2563..3501
                     /gene="fruK"
                     /locus_tag="OO609_001568"
     CDS             2563..3501
                     /gene="fruK"
                     /locus_tag="OO609_001568"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6633034.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3518..5209
                     /gene="fruA"
                     /locus_tag="OO609_001569"
     CDS             3518..5209
                     /gene="fruA"
                     /locus_tag="OO609_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6633035.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5633..6574
                     /locus_tag="OO609_001570"
     CDS             5633..6574
                     /locus_tag="OO609_001570"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6633036.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6562..7500
                     /gene="psuG"
                     /locus_tag="OO609_001571"
     CDS             6562..7500
                     /gene="psuG"
                     /locus_tag="OO609_001571"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6633037.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7594..8844
                     /locus_tag="OO609_001572"
     CDS             7594..8844
                     /locus_tag="OO609_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6633038.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8915..9609)
                     /gene="yeiL"
                     /locus_tag="OO609_001573"
                     /pseudo
     CDS             complement(8915..9609)
                     /gene="yeiL"
                     /locus_tag="OO609_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9740..10681
                     /gene="rihB"
                     /locus_tag="OO609_001574"
     CDS             9740..10681
                     /gene="rihB"
                     /locus_tag="OO609_001574"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6633039.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10781..12031
                     /locus_tag="OO609_001575"
     CDS             10781..12031
                     /locus_tag="OO609_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6633040.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12087..13175)
                     /locus_tag="OO609_001576"
     CDS             complement(12087..13175)
                     /locus_tag="OO609_001576"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6633041.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13178..14035)
                     /gene="nfo"
                     /locus_tag="OO609_001577"
     CDS             complement(13178..14035)
                     /gene="nfo"
                     /locus_tag="OO609_001577"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6633042.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14109..15158)
                     /locus_tag="OO609_001578"
     CDS             complement(14109..15158)
                     /locus_tag="OO609_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6633043.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15257..16138
                     /locus_tag="OO609_001579"
     CDS             15257..16138
                     /locus_tag="OO609_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6633044.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16344..17813
                     /gene="lysP"
                     /locus_tag="OO609_001580"
     CDS             16344..17813
                     /gene="lysP"
                     /locus_tag="OO609_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6633045.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18105..20096
                     /gene="cirA"
                     /locus_tag="OO609_001581"
                     /pseudo
     CDS             18105..20096
                     /gene="cirA"
                     /locus_tag="OO609_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20128..20963)
                     /gene="yeiG"
                     /locus_tag="OO609_001582"
                     /pseudo
     CDS             complement(20128..20963)
                     /gene="yeiG"
                     /locus_tag="OO609_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21221..21889
                     /gene="folE"
                     /locus_tag="OO609_001583"
     CDS             21221..21889
                     /gene="folE"
                     /locus_tag="OO609_001583"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6633046.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21906..22982
                     /locus_tag="OO609_001584"
     CDS             21906..22982
                     /locus_tag="OO609_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6633047.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23124..24164
                     /gene="galS"
                     /locus_tag="OO609_001585"
     CDS             23124..24164
                     /gene="galS"
                     /locus_tag="OO609_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6633048.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24444..25442
                     /gene="mglB"
                     /locus_tag="OO609_001586"
     CDS             24444..25442
                     /gene="mglB"
                     /locus_tag="OO609_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6633049.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25503..27023
                     /gene="mglA"
                     /locus_tag="OO609_001587"
     CDS             25503..27023
                     /gene="mglA"
                     /locus_tag="OO609_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6633050.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27039..28049
                     /gene="mglC"
                     /locus_tag="OO609_001588"
     CDS             27039..28049
                     /gene="mglC"
                     /locus_tag="OO609_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6633051.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28085..29298)
                     /gene="preA"
                     /locus_tag="OO609_001589"
                     /pseudo
     CDS             complement(28085..29298)
                     /gene="preA"
                     /locus_tag="OO609_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29292..30530)
                     /gene="preT"
                     /locus_tag="OO609_001590"
     CDS             complement(29292..30530)
                     /gene="preT"
                     /locus_tag="OO609_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6633052.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30724..30963)
                     /locus_tag="OO609_001591"
     CDS             complement(30724..30963)
                     /locus_tag="OO609_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6633053.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(30966..31685)
                     /gene="sanA"
                     /locus_tag="OO609_001592"
     CDS             complement(30966..31685)
                     /gene="sanA"
                     /locus_tag="OO609_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6633054.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31835..32719)
                     /gene="cdd"
                     /locus_tag="OO609_001593"
     CDS             complement(31835..32719)
                     /gene="cdd"
                     /locus_tag="OO609_001593"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6633055.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32849..33544)
                     /locus_tag="OO609_001594"
     CDS             complement(32849..33544)
                     /locus_tag="OO609_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6633056.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33541..33939)
                     /locus_tag="OO609_001595"
     CDS             complement(33541..33939)
                     /locus_tag="OO609_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6633057.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34178..35125
                     /gene="dusC"
                     /locus_tag="OO609_001596"
     CDS             34178..35125
                     /gene="dusC"
                     /locus_tag="OO609_001596"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6633058.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         8673 a         8614 c         9336 g         8687 t
ORIGIN      
        1 gatgatccag cccacgcgcg acgtgttggt atagagcgtg gtggctgaac ctgcctgacc
       61 gggcatcaaa tcctgaaaat agagcatccc gatgccgccc agaatgccaa taaaaatagc
      121 atttagcagc tgcaagccca acagaatggc aggtgaatgc gccatcagca ttcctgcgta
      181 aaaacagacg ccacccacgg cagcaacgcg cattaagaaa cgcttaccca gacgtttggc
      241 gaaatatccg gcaatcaaca tcgtcgggat ttccagcccg gcggcggtcc ccatcatcac
      301 accggccagt ttctcgggaa gatgcagttc gttgataata aatagcggca tgttgatgat
      361 gtacaggctg ttcgagcccc acatcaatgt acaaatgaca aacagcagta gcgtatcgcg
      421 acggttacgg cgcggcgctt caaccgtgcc ggtcgccagc ggaagctctt tttgcatcga
      481 cggtaaaaac agccacacca tcacgccgca aacaataaac gctaccgctg cgctcagata
      541 cattaccgta aagctgaaac ccatcgctaa cgcataagcc agcggtgggc caatgaccca
      601 tgccagtgaa acctgagcgc gtaaaaaaga gctgaacatc accgcctcac gtccggtttt
      661 gtcggcatgt tcacgggcaa gggcaaacat ttgcgggtta gcggtcgagc caaagctgct
      721 aagaaagacg ccaacgaata gcaaaacaaa gtagttgcga ttccaagcaa aaagggtgca
      781 ggccagcacg cctaacaggc agcaaaagac aatcagcgat ttgcgatcgc cgcgcttatc
      841 agagcgccca gcgagaaact gacttaccag aatcccaatg acagcgctgc cggtaaagaa
      901 gaatcccacc atcgccggac gggcatgtac ttcatcggta aggaaaatac tgagtgtcgg
      961 ggtttgcaga gcacccgcaa taccggtgag aaaggcaacg attaaaaacg ccgtcgaggt
     1021 caggtcaaac gatttcgcgc tggagactgc gggggagtta tgcatgctgg tcgatcactg
     1081 gaagaggaag acgcggagtt tacgctgctt gtcagaaaat aaacagacag cgaaatcaaa
     1141 agcgccacga ataatcaaaa tgccatttca ctgttgctga ttggctgctg acgctgctga
     1201 aatgaaattg ctgataacgg attttcccat cagcaattag gaaaaatgac aaaaaattgt
     1261 gcagcacatc aaacttttgc tcataacttt acggctttcc ttgcgtgctg aaacagaagt
     1321 attatgcttt cttgaaacgt ttcagcgcga tcttgtcttt aaccctaagc taggttggcg
     1381 ctttttttct catagaggct gaatcgtttc aattcagcaa gagaggagaa ctatgttcca
     1441 gttatccgta caggacatcc atccgggcga aaaggccgga gacaaagaag aggcgattcg
     1501 ccaggtcgct gcggcgctgg tgcaggccgg taatgtagca gaaggctacg tcaatggcat
     1561 gctggcgcgc gaacagcaaa cctcaacgtt cctcggcaat ggtattgcta ttccacacgg
     1621 cactaccgac acccgcgatc aggtgctgaa aaccggcgtt caggtatttc agttcccgga
     1681 aggcgtcacc tggggtgacg gtcaggtagc gtacgtggca atcggtattg ctgccagctc
     1741 ggataaacat ctgggcctgc tacgccagct gacccacgta ctgagcgatg attccgttgc
     1801 tgaacaactg aagtcagcaa caacagcaga agaacttcgc gcattactga tgggcgaaaa
     1861 gcagagtgag cagctgaagc tcgacaacga aatgctgacg ctggatatcg tcgccagcga
     1921 tctgctgact cttcaggcgc tgaacgctgc gcgtctgaaa gaggcgaggg cagttgacgc
     1981 cactttcgtc accaaagcca tcaatgaaca accgctgaat ctcggacagg gtatctggct
     2041 gagcgatagc gccgaaggca atctgcgtag tgcgattgcg gtaagccgtg cggcaaatgc
     2101 ttttgatgtg gacggcgaaa cggcagccat gctggtgagt gtggcgatga atgacgatca
     2161 gcccatcgcg gttcttaagc gtctcgctga tttgttgctc gacaataaag ctgaccgctt
     2221 gctgaaagcg gatgcggcaa cgttgctggc gctgctgacc agcgatgatg cgccgaccga
     2281 cgacgtgtta agcgcggagt ttgtggtgcg caatgaacac ggcctgcatg ctcgtccagg
     2341 taccatgctg gtcaatacca ttaaacaatt taacagtgat attaccgtga caaaccttga
     2401 tggcaccggc aaaccggcaa acggacgtag tctgatgaaa gttgtggcac ttggcgttaa
     2461 gaaaggtcat cgcctacgct ttaccgccca gggtgcagat gctgaacagg cgctgaaagc
     2521 cattggcgac gctatcgctg ctggtcttgg ggagggcgca taatgagcag acgtgttgct
     2581 acgatcaccc ttaatccggc ttatgacctt gttggtttct gcccggaaat tgaacgcggc
     2641 gaagtgaacc tggtgaaaac caccggtctg catgcggcgg gtaaaggcat caacgtggcc
     2701 aaagtattaa aagacctggg aattgatgtc accgttggcg gcttcctggg taaagacaat
     2761 caggatggtt ttcagcaact gttcagcgag ctgggcattg ccaaccgttt ccaggttgta
     2821 caggggcgca cccgaattaa cgttaagctg acggaaaaag acggcgaagt gaccgacttc
     2881 aacttctcgg gtttcgaagt cacccccgcc gactgggaac gctttgtgac tgattctctg
     2941 agctggctcg gtcagttcga tatggtctgt gtcagcggaa gcttaccgtc aggcgtcagc
     3001 ccggaagcgt tcaccgactg gatgactcgc ctgcgtagtc agtgtccttg cattatcttt
     3061 gatagtagcc gtgaagcgtt agtagcaggt ttgaaagcgg caccgtggct ggtgaaacct
     3121 aaccgccgcg agttggaaat ctgggcaggc cgtaaactgc ctgaaatgaa agatgtgatt
     3181 gaagctgcac atgcgctacg tgaacaaggt attgcgcatg ttgttatttc actgggtgcc
     3241 gaaggcgcgc tttgggttaa tgcctccggc gaatggatcg ctaaaccacc gtcagtcgat
     3301 gtcgtaagca ccgttggcgc aggggattct atggttggtg gcctgattta tggcttgctg
     3361 atgcgtgaat ccagtgaaca cacactgcgt ctggcgacag ctgttgcagc cctggcggta
     3421 agtcaaagca atgtgggtat taccgatcgt ccgcagttgg ccgcaatgat ggcgcgcgtc
     3481 gacttacaac cttttaactg acagcaggag aggcataatg aaaacgctgc tgattattga
     3541 cgctaatctc ggtcaggcac gcgcctatat ggcgaagacc ctgctgggcg cggcggcgcg
     3601 aaaagcaaaa ctggaaatca tcgacaatcc gaacgacgca gaaatggcga ttgttctcgg
     3661 tgattccatc ccgaatgaca gcgcgctgaa cggtaaaaat gtctggctgg gtgatatttc
     3721 ccgggcagtt gcgcaccctg agctgttcct gagtgaagcc aaaggccatg cgaaacctta
     3781 cactgcgccg gtcactgcga cagcaccggt tgccgccagc ggtccgaaac gcgtagttgc
     3841 ggtgactgct tgcccgactg gcgtagcaca cacctttatg gcggctgaag ccattgaaac
     3901 cgaagcgaaa aaacgtggct ggtgggtgaa agttgaaacc cgtggttctg ttggcgcggg
     3961 taatgcaatc actcccgagg aagtagccgc agcggatctg gtgattgtgg cggcagatat
     4021 cgaagtggat ctggcgaaat ttgctggtaa accgatgtat cgtacctcta ccggtctggc
     4081 gctgaagaaa acctcgcagg aactggataa agcggttgct gaagcaacgc cgtatgaacc
     4141 ggcgggcaaa gctcaaacgg cgaccactga aggtaagaaa gagagtgcag gcgcttatcg
     4201 tcacttgcta acgggcgtct cttacatgct gccgatggtc gttgctggtg gtctgtgtat
     4261 cgcgctttct tttgcttttg gtatcgaagc gtttaaagag ccgggtacgt tggcagcggc
     4321 gctgatgcag attggtggtg gttcagcctt tgcgctgatg gtgccggtac tggcaggtta
     4381 tattgccttc tccattgccg atcgtccggg cctcacgccg ggtctgattg gcggtatgct
     4441 ggcggtcagc accggttctg gcttcattgg cggtattatt gcgggcttcc tggctggtta
     4501 cattgcgaag ttaatcagta cgcaactgaa actgccgcag agtatggagg cgctgaaacc
     4561 gatcctgatc attccgctaa tttccagtct ggtggtcggt ctggcgatga tctacctgat
     4621 cggtaaacca gttgctggca ttctcgaagg gctgactcac tggctgcaga ccatggggac
     4681 tgcgaatgcg gttctgctgg gggcgatcct cggtggcatg atgtgtactg acatgggcgg
     4741 tccggtaaac aaagcagcgt acgcattcgg tgtgggtctg ctgagtactc aaacctatgg
     4801 cccgatggcg gcgattatgg cggcaggtat ggtgccaccg ctggcaatgg gcctggcaac
     4861 aatggtggcg cgtcgcaaat tcgacaaagc gcagcaggaa ggtggcaaag ccgctctggt
     4921 attgggactg tgcttcattt cggaaggtgc aattccgttt gctgctcgtg atccgatgcg
     4981 tgtgctgccg tgctgtatcg tgggtggggc gctgactggc gcaatctcaa tggcgattgg
     5041 tgcgaaactg atggcaccgc acggtggtct gtttgttctg ctgatccctg gcgctattac
     5101 gccggtattg ggttacctgg tagcaattat tgccggtacg ctggtggcgg gtttggccta
     5161 tgccttcctg aaacgtccgg aagtggacgc agtagcgaaa gcagcgtaat aaaaggtgtg
     5221 ttacagggca gaaatttcct gccctgtaac atttgatgag cgcaaagaga atgaccagct
     5281 tgtgaatgta attttatgaa tttaaatggt tttatctttc agtagaaact tcaaacgttc
     5341 atcaaacact aaggttattt cctcaagggc agattatttt ctgccctttt ttagtgttcc
     5401 tgttctgtgc atcccgtcac aaattcctcg cttttgtaat tcaggcattt aatcgcattt
     5461 tgtgagcctg ctcaaatctg cccattttgc ctctctggcg gtaatttact ttacgtaaaa
     5521 catgcatact atgagcacat gtttaaatat gaacacatgt ttaaaccgtc tttgcagtat
     5581 ctatattctt gtggattgac agggttggta attttttacc agaggacgtg ctatgcgcga
     5641 aaaggattat gtcgtaatta taggttcggc gaatattgat gtcgccggat attcacatga
     5701 atcattaaat tatgcggatt caaatccagg taaaataaaa tttacgcctg gtggagtagg
     5761 gcgcaatatt gcacaaaacc tggcgttgct gggtaacaaa gcctggctac tgagcgccgt
     5821 aggcagtgat ttttatggtc aatcgctgct aacgcaaacc aatcaatctg gtgtttatgt
     5881 cgataaatgc ctgattgtgc cgggagaaaa tacgtcgagt tatttatcat tactcgataa
     5941 taccggtgaa atgctggttg ctataaatga catgaatatt agcaacgcta ttacagctga
     6001 atatctcgca cagcaccgtg aatttattca gagggcaaag gtcattgtcg cggactgtaa
     6061 tatcagtgaa gaggcactgg catggattct ggataatgcc gccaacgtac ccgtatttgt
     6121 cgatccggtt tcagcgtgga aatgtgtcaa agtgcgcgac cgtctaaatc agaaccacac
     6181 tctgaagcca aaccgccttg aagcggaaac cctgagtggg attgcgctgt cagggcgtga
     6241 cgatgtggca aaagttgctg cctggttcca tcaacatggc ctgaaccgac tggtattgag
     6301 catgggcggc gacggcgttt attacagcga tatcagcggt gaaaatggct ggtctgcgcc
     6361 gatcaaaacc aatgttatta atgttaccgg agcgggcgat gccatgatgg cgggacttgc
     6421 ttcgtgttgg gtagacggaa tgccgtttgc cgaatctgtt cgtttcgcac agggatgttc
     6481 gtcaatggcg ctctcctgtg aatacaccaa taaccccgat ttatcgattg ccaacgttat
     6541 atcgttagtg gagaacgcag aatgtctgaa ttaaaaattt cccctgaatt attacaaatt
     6601 tctccggaag tgcaggacgc tttaaaaaac aaaaaaccgg ttgtggcgct ggaatcgacc
     6661 attatttctc acgggatgcc gttcccacaa aatgcccaga ccgcaattga agtagaagaa
     6721 actattcgta aacagggcgc agtacctgcc actatcgcca ttattggcgg cgtgatgaaa
     6781 gtgggtttaa gcaaagaaaa aattgaatta ctgggtcgtg aagggcataa cgtgaccaaa
     6841 gttagtcgtc gcgatttacc ttttgttgtt gccgccggaa aaaatggcgc aaccactgtg
     6901 gcttcaacga tgattattgc ggcgcttgcc ggaattaaag tatttgccac cgggggaatt
     6961 ggtggtgtgc atcgcggggc ggaacatacc ttcgatattt ctgccgattt gcaagaactg
     7021 gcaaatacta atgtcaccgt tgtttgtgcc ggggcgaaat ctattctcga tttaggatta
     7081 accactgagt atttagaaac cttcggtgtg ccgttaattg gctatcagac taaagcgctg
     7141 cctgcgtttt tctgccgtac cagctcgttt gacgtcagca ttcgtctcga cagcgccagt
     7201 gaaattgccc gtgcaatggc ggtgaaatgg caaagcgggc tgaacggtgg cctcgtggta
     7261 gcgaacccga tcccggaaca gtttgcgatg ccggaggaat ctatcaatgc agccatagat
     7321 caagccgtcg ccgaagccga agagcagggc gttattggta aagaaagtac accgttcctg
     7381 ctggcgcgcg ttgctgagct gaccagcggt gacagcctga aatccaatat ccagctggtg
     7441 ttcaacaacg ccattctggc gagtgaaatt gccaaagaat accagcgtct cgcgggttaa
     7501 tttctgacgg gcagaaagcc gtcgcccgta ctcatcaagc ataccaaaac ctggcgctga
     7561 cgccgggttt actggcatga agggaaaatc attatggata taatgagaag tgttgtgggg
     7621 atggtggtgt tactggcaat agcatttctg ttgtcagtga ataaaaagag catcagtttg
     7681 cgcacggttg gagccgcact gctgctgcaa atcgctattg gtggcatcat gctctacttc
     7741 ccaccgggaa aatgggcagt agaacaggcg gcattaggcg ttcataaagt gatgtcttac
     7801 agtgatgccg gtagcgcctt catttttggt tcgctggttg ggccgaaaat ggatgtcctg
     7861 tttgacggtg cgggttttat cttcgccttt cgcgtacttc cggcgattat tttcgttact
     7921 gcgctcatca gtctgctgta ctacattggc gtgatggggc tgctgattcg catccttggc
     7981 agcattttcc agaaagccct caacatcagc aaaatcgaat cttttgttgc ggttaccact
     8041 attttcctcg ggcaaaatga gatcccggcg atcgttaaac cgtttatcga tcgcgtgaat
     8101 cgcaacgagt tgtttaccgc aatttgtagc gggatggcgt ccattgctgg ttcggtgatg
     8161 attggttatg ccggaatggg cgtaccaatt gactacctgt tagcggcatc gctgatggcg
     8221 atccccggcg gtattttgtt tgcacgtatt cttagcccgg caaccgagcc ttcgcaggtc
     8281 acatttgaaa atctgtcgtt cagcgaaacg ccgccaaaaa gctttatcga agcggcggcg
     8341 agcggtgcga tgaccgggct gaaaatcgcc gctggtgtag cgacggtggt aatggcgttt
     8401 gtcgcaatta ttgcgctgat caacggcatt atcggcggaa ttggcggctg gtttggtttc
     8461 gccaatgcct ctctggaaag tatttttggc tatgtgctgg caccgctggc gtggatcatg
     8521 ggtgtggact ggagtgatgc caaccttgct ggtagcctga ttgggcagaa actggcgatt
     8581 aacgaattcg tcgcttacct gagtttctcc ccatacctgc aaacgggcgg cacgctggaa
     8641 gtgaaaaccg tggcgattat atcctttgcg ctttgtggtt ttgctaactt tggttctatc
     8701 ggtgttgtcg ttggcgcatt ttcggctatt tcgccaaaac gtgctccaga aatcgcccag
     8761 cttggtttac gggcgctggc agcagcaacg ctttccaacc tgatgagtgc gactattgcc
     8821 gggttcttta ttggtctggc gtaatgctcg atttgtagac cggataaaac gtattaagcg
     8881 ttgcatccgg caatcgcgct attgcaaata atttttactg catcatcccg gagaatttat
     8941 tctccgggtc catctccagc gccagtcctg acaactgctt tctgttttta attagatatc
     9001 ctttctttta attaataaac cgtcgtgaat gaattgcgcg agaacatata aaagatgtcg
     9061 ataagaaacg cctaaatact ctgccgcttg cgtatgcttt tcgtgataaa gatcaccttc
     9121 ttgtgagagt aatataaatg ctgccaggcg attcactaac ggaaatgatt gattctgagt
     9181 taaagaaaca atattacgat aatttttatg acttaaggtg acgcaaagtt ttcgtaaaaa
     9241 tagggtgtcg tttaataaca gcggacggta atgtttcata gggagcgcaa ggcaccaaca
     9301 ctcttcaata gcctgcaccg cacgcggttc atggtctttg tcgattaact caatctcgcc
     9361 aataaaacag ggagcggcaa agaaatcgat cagcgacacg cgaccattgg caagcgtggc
     9421 gtaaagcctg gcgcggcctc gcgtcagata aaacagccat gacggctgtt gcccttcctg
     9481 cacaatgtag tcacgcgcta aaaaatgaaa cagccgcgta tcggctgaaa catccgttga
     9541 aaagcaatcc ttaaacgcag attcactcat caattgctgc ttaaggtcat tattgtggat
     9601 ttctttcacg atgtttatcc gctatgagat ttctcatatt tagcatacgt acgctgttgt
     9661 taagattcaa tcattggccc ggtcgaattt tctttgctgt agaaaaaaat aacgcaactg
     9721 gaaacagagg aaataaacaa tggaaaagag aaaaattatt ctggattgtg agccgggtca
     9781 tgatgatgct attgcaatga tgatggcagc gaaacatccg gcaatagatt tattaggcat
     9841 cactattgta gcgggtaatc agacgcttga taaaacatta attaatggcc tgaatgtttg
     9901 ccagaaactg gagattaatg ttccggttta tgcggggatg ccgcaaccca ttatgcgtaa
     9961 acaaatcgtt gccgataata ttcacggtga aaccggactg gatggcccgg tattcgagcc
    10021 gctgacccgc caggcagaaa gtacccatgc ggtgaaatat atcatcgata ccctgatggc
    10081 aagcgatggc gatatcactc tggtgccggt tggtccgctt tcaaatatcg cggtggcaat
    10141 gcgtatgcaa cccgcaatcc tgcccaaaat ccgtgaaatt gtgctaatgg gcggcgctta
    10201 cggtacaggc aacttcaccc catctgccga gttcaatatc tttgccgacc cggaagccgc
    10261 ccgcgtggtg ttcacctccg gcgttccatt agtgatgatg ggcctcgatc ttaccaacca
    10321 gaccgtttgc accccggatg taattgctcg gatggaaagg gtaggcggcc ctgccggaga
    10381 gctgttcagc gacatcatga acttcactct caaaacgcag ttcgaatact acggcctggc
    10441 tggcggcccg gtgcatgacg ccacctgcat tggttatctg atcaaccctg atggcattaa
    10501 aacccaggat atgtacgtcg aagtggacgt caacagcggc ccttgctacg ggcgtacggt
    10561 ctgcgacgaa ctgggcgttc ttggcaagcc cgctaatacc aaagtcggca tcactattga
    10621 taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc ggctacatca aaacccatta
    10681 agtgttgata acaagccggg acagtatcgc aagatgcagg cccggctatt tctgatgggc
    10741 agaaagccgt cgcccaaaat gacatgaaga gataatcact atggatgtca tgagaagtgt
    10801 tctgggaatg gtggtactgc tggcaattgc gtttttactg tcagtaaaca agaagaagat
    10861 cagcctgcgt accgttggcg cggcgttagt gttacaggtc gtgattggcg ggattatgct
    10921 ttggttaccg ccagggcgtt gggtcgcaga aaaagtcgct tttggcgtcc acaaagtgat
    10981 ggcgtacagc gacgcaggta gcgcatttat cttcggttct ctggtcggac cgaaaatgga
    11041 taccttattt gatggcgcag gatttatctt tggtttcagg gtattaccgg caattatctt
    11101 cgtcactgca ctggtgagta ttctctacta catcggtgtg atggggatct taattcgcat
    11161 tctcggcggt atcttccaga aagctttaaa tatcagcaag atcgagtcat tcgtcgcggt
    11221 caccaccatt ttcctcgggc aaaacgaaat tccggcgatc gtcaaaccct ttatcgatcg
    11281 tctgaatcgc aatgaattat ttacagcgat ttgtagtggc atggcctcga ttgctggttc
    11341 gacaatgatt ggttacgccg cactgggcgt acctgtggaa tatttgctgg cggcatcgtt
    11401 aatggcgatc cctggcggga tcttgtttgc ccgcctgtta agcccggcta cggaatcttc
    11461 gcaggtttct tttaataacc tctctttcac cgaaacaccg ccaaaaagca ttattgaagc
    11521 cgatgcgaca ggggcaatga ccgggctgaa aatcgccgcc ggtgtagcga cagttgttat
    11581 ggcatttgtc gccatcattg cgttaattaa cggtattatc ggcggcgttg gcggctggtt
    11641 tggttttgaa catgcctcgc tggagtccat tttaggttac ctgctggccc cacttgcgtg
    11701 ggtaatgggt gttgactgga gtgatgcaaa tcttgccggg agtttgattg gacagaaact
    11761 ggcaataaat gaatttgtcg cttatctcaa tttctcaccc tatctgcaaa cggctggcac
    11821 tctggatgct aaaaccgtgg cgattatttc cttcgcgttg tgcggtttcg ctaactttgg
    11881 ttctatcggg gtggtggtgg gggcgttttc tgcggttgcg ccacaccgtg cgccggaaat
    11941 cgcccagctt ggtttacgcg cgctggcggc ggcgacactt tctaatctga tgagtgccac
    12001 cattgccggg ttctttattg gtttagcgta gtgtgcaatt tatgagccgg attagatgcg
    12061 gtcaagcgtc gaatccggca acattgttac accattggca ctaatgacag cgcgttatcg
    12121 gcagacaggg tagggttgtt taagctgccg ctggcgcgtg aaattgccgc gcaggccatc
    12181 gcaaaacggg cgctgtcacg gaaattgttt ccctccagaa agctatatac cagacccgcc
    12241 ataaaaccat cgtcagcacc aaaactgtct accgtcgtat gcgctggcac agtcagcaaa
    12301 aattgttcac catccttttc gctgcaataa actgactcat cgggcaaata aacaaacagt
    12361 tgctgaacac cttgctgatg caatgcattc actgcggcat tacggtcagc atcgctggtg
    12421 atcgcctgtc cccataaaat ttccagctcc ggtaaagtgg gtttcagggt gtgaatatgc
    12481 gccagccagt gtttgatttt gcccgctttg aattctgaaa cggtatcgac aaacaccggg
    12541 atctcatcag cgagggtaaa gacccattcc agcgcctcgg ctgtcaggtt gcaatcagcc
    12601 agtaccacgc ccgcatgacg aagtaaatcg cgcgacccgt tcagtagttg cggtgtcaac
    12661 tgttccagca gatgggtatc gttaattgcc agcacggttt gatcgtctcg attggcaatt
    12721 gccagatacg tcgatgtgct ttgaccatgc aaacgaacgc agccggagac attcacgccg
    12781 gcacggcgcg tttcttccag gagcatttcg ccataaaagt catcgccaat cactgaaagc
    12841 aaatggacgt cacggcctaa cagcgccaga ttgtgggcga tattgcgtcc aacgccgcct
    12901 gccgaacaat gaattgtacc gggatgagaa gccgcttgcg ggtaatggat atccgccatc
    12961 ccgcgaatat ccatattgat tgtccccacc actacgcagt attcctgctc ggtgagaatg
    13021 taacctttgt ctttaatccg gcctttgcgc attaaatcca taatatgcgc cgcaacgcgc
    13081 gaacgactga tttgcagcat gtccgcaatt tcgttctgct gaatcagcgg gttacgccgt
    13141 aaaattgcaa ggatctcctt ttcccggtta tttatcttca ggctaccgct ttttcagttt
    13201 gttgcgcttt cagccaggcg atctcttctg cccagatatc cgggttgatg gtttcaagga
    13261 tcagcggaat gccgtcgaaa cggtcgttct gcataatcca gcggaacgca tcatgaccga
    13321 tattgccttc accgaggcta tgatggcggt caacgcggct gccaaaggta cttttcgcat
    13381 cgttaaggtg catcccgcgc aaatacttaa agccgacaat acgggcaaaa tcagcgaacg
    13441 ttttttcgca ttcggctgga gtacgcaaat catacccggc agcgaaagca tggcaggtat
    13501 caatgcagac gccgacgcgg gatttatctt ccacgccgtc gataatcgcc gcgagatgtt
    13561 cgaatttaaa ccctaagtta ctgccctgac cggcggtgtt ttcaatcacc gctgttacac
    13621 cttgagtttt atccagcgca atgttgatgg attcggcaat acgcgcaagg caatcctctt
    13681 ctgaaatctg catcagatgg ctgccagggt ggaagttgag caaagaaagc cccagttgtt
    13741 cgcaacgctg catttcatct ataaaggcat cgcgcgattt ttccagagct tcagtgaccg
    13801 gatgtccgag gttgatcaga taactgtcgt ggggaagaat ttgcgccgat gtgtagtgat
    13861 atttttcaca ggcggctttg aattcatcga tggtttgcgt cgtgagcggt gcggcacgcc
    13921 actgacgttg gtttttggtg aacaaggcaa acgcggttgc gtcgatttcg gcggcgcgaa
    13981 ttgcggcatt tgccagaccg ccagcagcac taacgtgcgc tccaatgtat ttcatgcgag
    14041 gactcctgtt aaacccgctg gaggaaaacg gtaatgatag cgggttaaca ggagcaagaa
    14101 gtagtggttt atgcgatgac gctttgaatc acatagttaa tcgcaccacc accaacaatc
    14161 agccaggcaa acagtaccag tgccatcagc agaggtttcg ccccagcttt tttcagcgcg
    14221 ctgacgtgag tggtcagacc cagcgccgcc atcgccattg ccagcaggaa ggtatccagc
    14281 gttaccagca tgttcaccac gctctgcggt aacaggtgga acgagttaaa gatggcaact
    14341 acgatgaaca agatggcaaa ccacggaata gtgattttgc ttttctcgcc gctgttcgcc
    14401 ccagacagct gtttaacacg cgccgccagc aggatgagga acggagccag catcatcacg
    14461 cgcagcattt tggaaataac tgctgcgttt tctgcatccg ggctgatggc atgacctgcc
    14521 gccaccacct gcgccacttc gtgcacagta gaaccaatgt agataccgaa agtttccgga
    14581 ctaaaccatt gagacatcag cggatatatc gccgggtaga ggaaaatcgc gacggtcccg
    14641 aagataacaa cggttgcaac agccacggtt actttactgg cttccgcttt cactaccggc
    14701 tcagtcgcca gtaccgcggc agcaccacag atactgctac cggcaccgat caaccaactg
    14761 gtgtgcttat ccagaccaaa cactttctgc cccaggaagc aagccagcag gaaggtactg
    14821 gacagcgtca gcacgtcaat gatgatccca ctgataccga catcggcaat ttgcgagaac
    14881 gtcagacgga agccataaag aatgataccc agacgtaata aatattgctt ggcaaacagc
    14941 acaccaccgt cacagctttt ccagatgtgc ggatagatgg tgttgcctaa aaccatcccc
    15001 aacaagattg cgagggtgag ggcactaaac ccggcacccg caaccgcggg aatggaacca
    15061 ccccacaggg cgaccccggt gataactgca ctcagggcta accccggaat aaaatgccac
    15121 agtgtacgat gttgtttctg taaggtgata ttcgtcataa ccctctcctt taaccgggca
    15181 taaggttacg actaactggt ttaaaaataa aattgattat atatttataa ttaatcttta
    15241 taagtggtaa gcgactatgc acatcaccct ccggcagttg gaagtttttg cagaagtatt
    15301 gaaaagtgga tcaaccaccc aggcgtcggt gatgctggcg ttgtcgcaat cagcagtgag
    15361 cgcagccttg actgacctgg aagggcagct tggcgttcaa ctgtttgatc gcgtggggaa
    15421 aagactggtt gttaatgaac acgggcggct gctctatccg cgagcgttgg cactgcttga
    15481 acaggcggtt gaaatcgaac aactgtttcg cgaagacaac ggcgcgattc gtatctatgc
    15541 cagtagtacc atcggtaact acattctgcc tgcagttatc gcccgttatc gccatgatta
    15601 tccgcagttg ccgattgaac ttagcgttgg gaacagccag gacgtgatgc aagcggtgct
    15661 ggatttccgc gttgatattg gctttattga aggaccgtgc cacagcactg aaatcatttc
    15721 tgaaccgtgg ctggaagacg agctggtggt tttcgccgcg ccgacttcgc cgttggctcg
    15781 tggtccggtc accttagaac agctggccgc tgcgccgtgg atcctgcgtg aacgcggttc
    15841 cggcacgcgg gatattgtcg attatctgtt gctgtcacat ttaccgaagt ttgagatggc
    15901 gatggaatta ggtaactccg aggcaatcaa acatgcggtg cgtcatgggt tgggaattag
    15961 ttgcctgtcg cgacgtgtga ttgaagatca attgcaggca ggcacattaa gtgaagttgc
    16021 ggtccctctg ccgcgcctga tgcgtacgtt gtggcgtata catcatcggc aaaaacacct
    16081 ttccaacgcg ctacggcgct ttctggacta ttgcgatccc gcaaatgtgc cgcgttaagt
    16141 tgctgtacaa gaacatgctg gtgctgtgtc gatttcgtga cgcagcgcct tcagcatgca
    16201 ttcgccagaa aagagattgg ctgctttact tataatccct gggcgatcat gaaggtgtct
    16261 tataaccgtg tatttctgcc ggaaggattg ccaatcgtct gctacaatcg cgccatcatt
    16321 tttaagatgg atagcatttt tgtatgggtt ccgaaactaa aactacagaa gcgccgggct
    16381 tacgccgtga attaaaggcg cgtcacctga cgatgattgc cattggcggt tccatcggta
    16441 caggtctttt tgttgcctct ggcgcaacga tttctcaggc aggtccgggc ggggcattgc
    16501 tctcgtatat gctgattggc ctgatggttt acttcctgat gaccagtctc ggtgaattgg
    16561 ctgcatatat gccggtttcc ggttcgtttg ccacttacgg tcagaactat gttgaagaag
    16621 gctttggctt cgcgctgggc tggaactact ggtacaactg ggcggtgact attgccgttg
    16681 acctggttgc agcacagctg gtcatgagct ggtggttccc ggatacaccg ggctggatct
    16741 ggagtgcgtt gttcctcggc gttatcttcc tgttgaacta catctctgtt cgcggctttg
    16801 gtgaagcgga atactggttc tcactgatca aagtcacgac agttattgtc tttatcatcg
    16861 ttggtgtgct gatgattatc ggtatcttca aaggcgcgca gcctgcgggc tggagcaact
    16921 ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc gatgatcggc gtagctatga
    16981 ttgtcggctt ctctttccag ggaaccgagc tgatcggtat tgctgcaggc gagtccgaag
    17041 atccggcgaa aaacattcca cgcgcggtac gtcaggtatt ctggcgaatc ctgttgttct
    17101 atgtgttcgc gatcctgatt atcagcctga ttattccgta taccgatccg agcctgctgc
    17161 gtaacgatgt taaagacatc agcgtcagtc cgttcaccct ggtgttccag cacgcgggtc
    17221 tgctctctgc ggcggcggtg atgaacgcag ttattctgac ggcggtgctg tcagcaggta
    17281 actccggtat gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac ggtaaagcgc
    17341 cgcgcatttt cgctaaattg tcgcgtggtg gcgtgccgcg taatgcgctg tatgcgacga
    17401 cggtgattgc cggtttgtgc ttcctgacct ccatgtttgg caaccagacg gtatacctgt
    17461 ggctgctgaa cacctccggg atgacgggtt ttatcgcctg gctggggatt gccattagcc
    17521 actatcgctt ccgtcgcggt tacgtattgc agggacacga cattaacgat ctgccgtacc
    17581 gttcaggttt cttcccactg gggccgatct tcgcattcat tctgtgtctg attatcactt
    17641 tgggccagaa ctacgaagcg ttcctgaaag ataccattga ctggggcggc gtagcggcaa
    17701 cgtatattgg tatcccgctg ttcctgatta tttggttcgg ctacaagctg attaaaggaa
    17761 ctcacttcgt acgctacagc gaaatgaagt tcccgcagaa cgataagaaa taagtttcct
    17821 cccttccttg ctaagccctc tcaaccgaga gggctttttc aattccattt ccctgacaaa
    17881 tcatgcggat ataaaattta acatttggat tgataattgt tatcgtttgc attatcgtta
    17941 cgacgcaatc aaaaaaggct gacaaatcag aggctgttcc ggctttctgg gatgatcacc
    18001 tgcatacaaa taagtccacc gcgatgctgc cgtacgcaag gggacgtaaa gaagatgtga
    18061 gcgataccca ttttattttc gtagttacct catggagata tggaatgttt aggttgaacc
    18121 ctttcgtacg ggtcgggctg tgtttgtccg ctatttcttg tgcatggcct gtgttagcgg
    18181 tcgatgatga tggcgaaacg atggttgtca ctgcatcttc cgtggaacaa aatcttaaag
    18241 atgcacccgc cagtatcagc gtcattaccc aggaagacct gcagcgaaaa ccggtacaga
    18301 atctgaagga tgtcctcaaa gaagtgcctg gcgtacaact gacgaacgaa ggggataacc
    18361 gtaagggcgt tagtattcgt ggtctggaca gcagctacac cctgattctt gtcgacggta
    18421 aacgcgttaa ctcccgcaat gccgtcttcc gccacaatga tttcgatctg aactggatcc
    18481 cggtcgattc catcgaacgt attgaagtgg ttcgtggccc gatgtcgtcg ctatacggct
    18541 ccgatgcgct cggcggtgta gtgaatatca tcaccaaaaa aatcggtcag aaatggtcgg
    18601 gtaccgttac cgtcgatacc accattcagg aacatcgcga tcgcggtgat acctataacg
    18661 gtcagttctt taccagtgga ccattaattg atggtgtgct gggaatgaaa gcttacggca
    18721 gcctggcaaa acgtgaaaag gatgacccgc aaaactcaac gaccaccgat accggagaaa
    18781 cgccgcgtat tgaaggattc tccagccgcg acggcaatgt cgaatttgcc tagacaccga
    18841 atcaaaatca cgattttact gccggatacg gtttcgaccg tcaggatcgt gattccgatt
    18901 cgctggacaa aaaccgcctg gaacgccaga actactgcgt cagccataat gggcgttggg
    18961 attacggcac cagcgaactg aaatcctacg gtgagaaagt cgagaacaaa aaccctggca
    19021 acagcagccc gataacttcc gaaagcaata cggtcgacgg caaatacacg ttgccgctga
    19081 cggcgattaa tcagtttctc acggttggcg gtgaatggcg tcacgacaaa cttagcgatg
    19141 cggtgaacct gaccggggga accagctcca aaacgtctgc cagccagtac gcgctgtttg
    19201 tggaagatga atggcggatc ttcgagccgc tggcgctgac gaccggcgtg cgtatggacg
    19261 atcacgaaac ctacggtgaa cactggagtc cgcgtgccta catggtttat aacgccaccg
    19321 acaccgtaac ggtgaaaggg ggctgggcga cggcatttaa agcaccttct ctgttgcaac
    19381 ttagccctga ctggacgagc aattcctgcc gtggcgcatg taagattgtg ggtagcccgg
    19441 atctgaaacc agaaaccagc gaaagttggg agctggggct ttactacatg ggtgaagaag
    19501 gctggctgga agggggtgaa tccagcgtta tcgttttccg taacgatgtg aaagatcgta
    19561 tcagcatcag ccgtacgtct gacgtcaacg ctgcaccggg ctaccaaaac tttgttggtt
    19621 ttgagacggg cgctaacgga cggcgcatac cggtatttag ctactacagc gttaacaaag
    19681 ctcgtattca gggcgtggaa accgaactga aaattccgtt caacgatgaa tggaaactgt
    19741 cgatcaacta cacctacaac gatggtcgtg atgtcagcaa cggcgaaaac aaaccgctat
    19801 ccgatctgcc gttccatact gctaacggta cgctggactg gaaaccgctg gcgctggaag
    19861 actggtcatt ctatgtttct ggtcactata ccgggcagaa acgcgccgac agcgcgacgg
    19921 ctaaaacacc gggcggttat accatctgga ataccggcgc ggcctggcag gtgactaaag
    19981 acgtcaaact gcgcgcaggc gtgctgaacc ttggcgacaa ggatctcagt cgtgacgact
    20041 acagctataa cgaagacgga cgtcgttact ttatggcagt ggattatcgc ttctgatgag
    20101 aagatgcccg gcgaaccggg cggactttca cttcagtaaa tactgcgcat ggaagcgcag
    20161 gtgatcctct ataaaagagg cgatgaagta gtaactgtga tcatatcccg gctgaatacg
    20221 cagcgtcatc ggccacgctt tctggcgcgc ggcttctgcc aataccgcag gttgcaactg
    20281 gtcggcaaga aactgatcat tatcgccctg atcgataagc gtcgggatcg catcctgcgc
    20341 gttactggca tacatcagcg cgcaactatc ccattccagc atgcattttt gtcctcacct
    20401 aaataggtgc taaacgcttt gattccccac gggacgctgc acggattcac aatgggcgca
    20461 aaggccgaaa cgctggtgta tttacccgga tttttcagcg ccataatcag cgcaccgtga
    20521 ccacccattg agtgaccgct aatggcgcag cggtcgctga cattaaattg cgactgaacc
    20581 agcgccggta attcatcgcg cagataatca tacatccggt aatgcgtcgc ccacggcggt
    20641 tgcgtggcat taagataaaa gcctgcgccc tggcctaaat cgtagccatc gtcgttggca
    20701 accttttcgc cgcgcgggct ggtgtctggc atcaccagta caatccccag ttccgccgct
    20761 acccgctggg cacccgcctt ggtggtgaag ttctcgtcat tgcaggttaa tccggaaagc
    20821 cagtacagca ctggtggcgg ggtgtgatca cgaggggggg ggagaaagat actgaacgtc
    20881 atcgggcagt ttaaggtact ggagtcgtgt cgccagcgtt gctgccagcc ttcaaaacag
    20941 cggtgctctt cgagcatttc catgcgtggc tccttgttgt gttgtttgca atttcttcat
    21001 cataccgttt attcaatgtg ctgtgagtaa ctttcacttc cgtatttgca taacgatgtt
    21061 ttaacatctg ctgatgaaag gcagcggcaa ttacaataat tatcgctgtg aatactggat
    21121 tatgtgcgcc gcctcacgca caataatcaa gctgtaaatc agcttaataa ctttgccccc
    21181 acgcagggtg gaggcgtcac acctgcagga gaaatcataa atgccatcac tcagtaaaga
    21241 agcggccctg gttcatgaag cgttagttgc gagaggactg gaaacaccgc tgcgcccgcc
    21301 cgtgcatgaa atggataacg aaacgcgcaa aagccttatt gctggtcata tgaccgaaat
    21361 catgcagctg ctgaatctcg acctggctga tgacagtttg atggaaacgc cgcatcgcat
    21421 cgctaaaatg tatgtcgatg aaattttctc cggtctggat tacgccaatt tcccgaaaat
    21481 caccctcatt gaaaacaaaa tgaaggtcga tgaaatggtc accgtgcgcg atatcactct
    21541 gaccagtacc tgtgaacacc attttgttac catcgatggc aaagcgacgg tggcttatat
    21601 cccgaaagat tcggtgatcg gtctgtcaaa aattaaccgt atcgtgcagt tctttgccca
    21661 gcgtccgcag gtgcaggaac gtctgacgca gcaaattctt attgcgctac aaacgctgct
    21721 gggcaccaat aacgtggctg tctcgatcga cgcggtgcat tactgcgtga aggcgcgtgg
    21781 catccgcgat gcaaccagtg ccacgacaac gacctctctt ggtggattgt tcaaatccag
    21841 tcagaatacg cgccacgagt ttctgcgcgc tgtgcgccat cacaactgat taaaaggcag
    21901 gaaccatgga gcgcaacgtc acgctcgatt ttgttcgcgg cgtcgccatt ctggggatcc
    21961 tgctattaaa catcagcgcc tttgggctac caaaggcggc ttatctcaat cccgcctggt
    22021 acgatgctat tacgccgcag gatgcatgga cctgggcatt tctcgatctc atcggccagg
    22081 tgaaattcct cacgcttttt gcgctgctgt ttggtgctgg cctgcaaatg ttgctgcccc
    22141 gtggcagacg ctggatccag tcgcggttaa cgctgttagt cttgttgggc tttattcacg
    22201 gtttattgtt ctgggacggc gatattctgc tggcttacgg gctggtgggg cttggcgttt
    22261 tgctgttatt ggggctgatt tccgacagcc agaccagccg cgcctggacg ccggatgcat
    22321 cggctatttt gtatgaaaaa tactggaagc ttcacggcgg cgttgaagcg atcagtaatc
    22381 gtgccgatgg tgttggcaac agtttactgg cactgggcgc acagtatggc tggcaactgg
    22441 cagggatgat gctcattggt gcggcgctga tgcgcagcgg ctggctgaaa gggcagttca
    22501 gcttacgtca ctatcgtcgt actggttttg tgctggtggc gattggggtg accattaacc
    22561 ttcctgccat cgccctgcaa tggcagctgg actgggcgta tcgctggtgt gccttcttac
    22621 ttcaaatgcc gcgggaactg agtgcgccgt ttcaggcgat tggctatgcg tcgctgtttt
    22681 atggcttctg gccgcaattg agccgcttta agctggtgct tgcgatcgcc tgcgtcggac
    22741 ggatggcgct gaccaactat ctattgcaaa cgctgatttg taccacgctt ttttaccacc
    22801 tcggtttgtt tatgcatttt gaccgcctgg agctgctggc gtttgttatt ccggtatggc
    22861 tggcgaatat cctcttctct gttatctggc tgcgttactt ccgccagggg ccggtggaat
    22921 ggctctggcg tcagttaact ttgcgtgctg ccggaccggc aatatctaaa acatcaagat
    22981 aacgatctgg attacattcg ttaacaaaac ggctgtaacc gtttccattg ctgtgactcg
    23041 attcacgaag tcctgtattc agtgctgaca aaatagccgc cagcaagcag tcatttactg
    23101 caatctcata acaggtagtg aatatgatca ccattcgtga tgtagcgcgt caggctggcg
    23161 tctctgtggc aacggtttcc cgggtgctca ataacagcac gctggtcagt gccgacacgc
    23221 gtgaagcagt aatgaaagcc gtgagtgagc tggattatcg gccaaacgcc aatgcccagg
    23281 cgctggcaac tcaggttagc gacaccattg gcgtggtggt gatggacgtt tctgatgcgt
    23341 ttttcggcgc gctggtaaaa gcggtggatc tggtcgccca gcagcatcag aaatacgtgc
    23401 taatcggcaa tagctatcat gaagcggaaa aagagcgtca cgccattgag gtgttaattc
    23461 gccagcgttg taatgcgttg attgttcact caaaagcatt gagtgacgat gaactggcgc
    23521 aatttatgga taacattccc ggtatggtgt taatcaaccg cgttgtgccg gggtacgccc
    23581 atcgttgcgt ttgcctggat aatctcagcg gtgcccgaat ggcgacgcgc atgttgctga
    23641 ataacggtca tcaacgtatt ggttatcttt cttccagcca cggcattgaa gatgacgcca
    23701 tgcgtaaagc aggctggatg agtgcgttga aagagcagga tattattccg ccggaaagct
    23761 ggattggcac tggtacgccg gacatgccgg gcggtgaggc ggcgatggtt gaactgctgg
    23821 ggcgcaatct acaacttacc gctgtatttg cttataacga caatatggcc gctggcgcac
    23881 tgacagcatt aaaagataat ggcattgcga ttccgttaca tctctcaatc atcggtttcg
    23941 atgatattcc catcgcccgt tacaccgacc cgcaattaac gaccgtgcgt tatcccattg
    24001 cttcaatggc taaattagcc accgaactgg ccttgcaggg ggcagcaggc aatattgatc
    24061 ctcgtgccag ccactgtttt atgccgacgt tagtgcgtcg ccattctgtc gcaacgcgcc
    24121 agaatgcggc ggcgatcact aactcaacaa atcaggcgat gtaaccgctt tcaatctgtg
    24181 agtgatttca cagtatctta acaatgtgat agctatgatt gcaccgtttt aatgttgtaa
    24241 cccgtatgta acagtgaata atcactttca ccgaggtaat agcgtcataa caacaattaa
    24301 agccgttttc tggagcgtta ccgggcatgg aagaacgaat tttaaaaagt gagcttcggc
    24361 gttcagtaac acttcattaa ctctactgcc ccgccgagca tttatctcaa gcactaccct
    24421 gcataagaaa aaccggagat accatgaata agaaggtgtt aaccctgtct gctgtgatgg
    24481 ccagcatgtt attcggtgcc gctgcacacg ctgctgatac tcgcattggt gtaacaatct
    24541 ataagtacga cgataacttt atgtctgtag tgcgcaaggc tattgagcaa gatgcgaaag
    24601 ccgcgccaga tgttcagctg ctgatgaatg attctcagaa tgaccagtcc aagcagaacg
    24661 atcagatcga cgtattgctg gcgaaagggg tgaaggcact ggcaatcaac ctggttgacc
    24721 cggcagctgc gggtacggtg attgagaaag cgcgtgggca aaacgtgccg gtggttttct
    24781 tcaacaaaga accgtctcgt aaggcgctgg atagctacga caaagcctac tacgttggca
    24841 ctgactccaa agagtccggc attattcagg gcgatttgat tgctaaacac tgggcggcga
    24901 atcagggttg ggatctgaac aaagacggtc agattcagtt cgtactgctg aaaggtgaac
    24961 cgggccatcc ggatgcagaa gcacgtacca cttacgtgat taaagaattg aacgataaag
    25021 gcatcaaaac tgaacagtta cagttagata ccgcaatgtg ggacaccgct caggcgaaag
    25081 ataagatgga cgcctggctg tctggcccga acgccaacaa aatcgaagtg gttatcgcca
    25141 acaacgatgc gatggcaatg ggcgcggtag aagcactgaa agcacacaac aagtccagca
    25201 ttccggtgtt tggcgtcgat gcgctgccag aagcgctggc gctggtgaaa tccggtgcac
    25261 tggcgggcac agtactgaac gatgctaaca accaggcgaa agcgaccttt gatctggcga
    25321 aaaacctggc cgatggcaaa ggtgcggctg atggcaccaa ctggaaaatc gacaacaaag
    25381 tggtccgcgt accttatgtt ggcgtagata aagacaactt ggctgaattc agcaagaaat
    25441 aagactgatt gggcgcagtc gtttactgcg cccttattgt atggccaaca aggtaaaatt
    25501 atatggtcag ctcaacgact ccgtcttccg gggaatactt gttggaaatg agcggtatca
    25561 acaagtcctt tcctggtgtt aaggcacttg ataacgttaa tttaaaagtc cggccacatt
    25621 ctatccatgc attaatgggg gaaaacggtg caggaaaatc gacattatta aaatgcctgt
    25681 ttggtattta tcaaaaagac tccggcacca ttttattcca gggtaaagag atcgatttcc
    25741 attccgccaa agaggcactg gaaaatggta tttcgatggt acaccaggag ttaaacctgg
    25801 tattacaacg ttcggtgatg gataacatgt ggctggggcg atatcccacc aaaggcatgt
    25861 ttgtcgatca ggacaaaatg taccgcgaaa ccaaagcgat ttttgatgaa ctggatattg
    25921 atatcgatcc gcgtgcgcgc gtcggcacat tatccgtttc gcaaatgcag atgatcgaaa
    25981 tcgccaaagc gttttcctat aacgcgaaaa ttgtgattat ggatgaaccg acttcttcgt
    26041 taaccgaaaa agaggtcaat catctgttca ctattattcg taaattaaaa gagcgcggct
    26101 gcggtattgt ttatatctcg cataaaatgg aagaaatctt ccagttatgt gatgaagtta
    26161 ccgtattgcg cgacggtcag tggatcgcca ccgaaccgct ggcaggactg acgatggaca
    26221 agatcatcgc catgatggtt gggcgttctc ttaatcagcg ttttcctgac aaagaaaaca
    26281 agccgggcga agtcatcctc gaggtgcgta acctaacgtc actgcgccag ccgtcgattc
    26341 gcgatgtctc gtttgatctg cataaagggg agatcctcgg tattgccggg ctggtggggg
    26401 cgaaacgtac cgatattgtt gagacgttat ttggtattcg cgagaaatcg gctggcacca
    26461 ttacgttgca cggcaaacag atcaataacc ataatgccaa cgaagccata aaccacggat
    26521 ttgcactggt aactgaggag cgccgctcaa cgggaattta tgcctatctg gatattggtt
    26581 ttaactcgtt aatttccaat attcgcaact acaaaaataa agttggttta ctggataact
    26641 cgcggatgaa aagcgatacc cagtgggtga ttgattcgat gcgggtaaaa acgccgggtc
    26701 atcggacgca aattggttcg ctctccggtg gtaatcagca aaaggtgatt attggtcgct
    26761 ggctactaac gcaaccagaa atattaatgc tcgatgaacc gacgcgcggt attgatgtcg
    26821 gggcgaagtt tgaaatttat cagttaattg ccgaactggc gaagaaaggc aaggggatta
    26881 ttattatctc ctctgaaatg cctgagttgt tagggataac agaccgtatt ctggtcatga
    26941 gcaatggtct cgtttccgga attgtcgata caaaaacaac aacgcaaaac gaaattctgc
    27001 gtcttgcgtc tttgcacctt taagattagg ggcttcccat gagtgcgtta aataagaaaa
    27061 gttttcttac ttacctgaaa gagggcggta tttacgtcgt tcttttagtt ttgctggcga
    27121 ttattatttt ccaggaccca acatttttaa gtctgttgaa cttaagtaat attctcaccc
    27181 agtcatcggt gcgtattatt atcgcgctcg gtgtggcagg gttaattgtc acccagggga
    27241 ccgatctttc tgctggtcgt caggtagggc tggcggcagt ggtggctgcg acattattgc
    27301 agtccatgga taacgccaac aaagtgttcc cggaaatggc gacgatgccg attgcgctgg
    27361 ttattctgat tgtctgtgcc attggtgcgg tgatcggttt gatcaacggt ctgattatcg
    27421 cttatctcaa cgtgacgccg ttcattacca cgctcggcac gatgatcatc gtctatggca
    27481 tcaactcgct ctattacgac tttgtcgggg cgtcgccaat ttctggtttt gacagtggct
    27541 tctctacctt tgctcagggc tttgtcgcgc tggggagttt ccgtctctct tacatcacct
    27601 tctacgcgtt gattgcggtg gcgttcgtct gggtgttgtg gaacaaaacc cgcttcggta
    27661 agaacatttt tgccattggc ggtaacccgg aagcggcaaa aatatctggt gtcaacgtcg
    27721 gcctgaacct gctgatgatc tacgcgttgt ctggcgtgtt ctatgccttt ggcgggatgt
    27781 tagaagccgg acgtatcggc tctgccacca acaacctcgg ctttatgtat gagctggatg
    27841 ctatcgcggc gtgcgtggta ggcggtgtat cgttcagcgg cggtgtgggg acggtgattg
    27901 gcgtggtgac cggggtaatt atttttaccg tcatcaacta tggcctgacg tatatcggcg
    27961 taaacccata ctggcagtac atcatcaaag gggcgattat tatcttcgcc gtagcgctgg
    28021 attcactgaa atacgcgcgt aagaagtgat gtgattgcgg atattgccgg atgcgtctct
    28081 ggttttacaa cgttaccggg tgttctttct cgcctttctt aaacttcact tccccgagct
    28141 caatacaacc caccgggcag acgtgaccac aaagcagaca acccacacat ttctcggtat
    28201 tacaatgcgg tgtgcgggtt ttctcgctcc attccatcgc ctggtgaccg ccgtcgtaac
    28261 aggaaatata acagcgtcca cagccaacac atttgtcaag attgatacgg ggatagacaa
    28321 tataactgcg gtctaaatct tccgccggga caatattgtt attcgccaga cctaccatct
    28381 cctgcaacga atcaaatccc tgatcggcga gataatgcga taacccgctc gccatatctt
    28441 ctacgatgcg atagccgtac tgcataatgc cggtggtgac ctgtaacgtt gctgcgccga
    28501 gcaataaaaa ctccgccgca tcttcccagg tttcaatgcc gccgataccg ctgattggga
    28561 aatcgcgcag ttctggatgg gtgcgcattt gctgaatgaa gcgcagggcg atcggtttta
    28621 ccgctttacc ggaatatccg gaaatactcg atttaccgtt aacgatcggc ataccgattt
    28681 tctgattaag atcgatattg gtgatggatt taacggtgtt aattgccgca atgccatctg
    28741 cgccgccgcg cttcgccgcc agcgccactt cgcacatatc accgatattc ggcgtcatct
    28801 tcgccagcat tggcagcgtg gaaccccgtt tcactgcccg acaatatttt tctaccagct
    28861 ccgggctttg cccgacatcg ctacccatcg catgagaagt catttgcgga caggagaagt
    28921 tacactcgat catatccgcg ccagctgctc ccattgctgc tcattttccc ccatgatcga
    28981 agcgatcaat actttgtccg ggtaatcttc cttcagccga cgcagggcgg tcagattctc
    29041 ttccaacgga tgttcagcaa tctgctccat atttttgaag ccgataaaac cggtatcttc
    29101 tttcaccaga tgatcaaaac gcggcgagac ttcgttggcg ataaaaaagc cgatcgtttt
    29161 aaacaccaca ccgccccaac ctgtgtcgta ggctttggca cacatctcat agcagttgcc
    29221 taccggcgaa gaagagaggc agaacgggtt gggaaagttc acgccgcaaa aagtaatcga
    29281 aagatctttc gttaacatga gcaagctccc tctaaatagt gatgaatcgc ctcggcggct
    29341 tctttcccgg ttttcacggc atagaccacg gttttgtcac cctcaacaat atcgccagca
    29401 gcaaagactt gcggatcgcg gatctgataa ttttgtgttt tgatggtgtt acgctgctgc
    29461 tctaactccg caaaggcatc cagtctggca tgctgaccga cggcgagaat aattttatct
    29521 gccgccatcg tcagttcgcc tgataaccgt acatgcttaa acgtgacttt attgccttcc
    29581 acggctactg gcgtgaatcc atcgatgatc gaaacgccca gctcccgggc gctggcaaac
    29641 tctttttcgc ttgccggaaa ctcatctaac tcttcacgtg ctacgcaagt taccgcctga
    29701 cagccgagaa ctttcagcgt gctggctacg tccatcgcga catcaccgcc gccgataatt
    29761 aatgcgcttt gtggaatgct gatatcgcct tgcgcctgtc gtacacgttg caggaagtcg
    29821 acggcaatct caacgtcgct atgctcaaac agcggtagcc cggaaccgct tgataacccc
    29881 acggtgacca gtaccgcgcg gttttccgct ttcagttgct ccagggtgag tgttttaccg
    29941 atttcgttat tgcacttaat ggtcacgccc attttttcaa tacgggcgat ctctgcatcc
    30001 agcactgatt gcggtaaccg gaattgcgga ataccgttac gcaaccagcc accggggtgc
    30061 gcttctttct cataaatcgt gacgtcataa ccctggtttg tcagtgtcac actggcctgc
    30121 aatccggcag gacctgcgcc aataatcgcg actttgccga gcgttttagt accgggctga
    30181 taaatttcca tcccggtttg ttgttcaaaa tcagtaacaa aacgctgtaa gcggccgata
    30241 tcaatgggag catcaacacc ggcacgggta caaccgcttt ggcataattt ctccgtcggg
    30301 cacactctgg cacaaacggc accgagggca ttattttcgc gaattgtctc ggcagcgcct
    30361 ttaaaattac gaaagtagat tgagcgaata aatttccccg gatcggtctg tgccgggcaa
    30421 gcctgactac agggagcgtc gtgacataat aaacagcgag aggcttcttt aatcgccagt
    30481 aacggcgtaa aagccggagt gagttcatcc agataatttt gttgcggcat gtgatcttcc
    30541 ttaatgctat gagtaattat ggcgaacgct aaataaatat tgcgtgctga tattaaggct
    30601 atgcgcgtgg ggaaatttaa gctgtgaaag gattcacaaa tagctgcggt tcagaatgtt
    30661 atatccgcag cttattgagt gaagtaataa ttattctcaa taattaacag aaaagagaaa
    30721 atattaacga tagaaaataa gataaacaat catgccgata gataaaattc cgaagccaat
    30781 atatgccacc atataagcga agaagagtcc gggatttttt gctcgccaga catacaccgg
    30841 ttccggtgca tttttaaccc gtttatcaat cgcgcctgcg cgccagcctt tccatgcttc
    30901 gcggaaacaa aatatcggga taaggaaaaa aacggcaacg acaaaaaact gctgtacatc
    30961 cataactact ttccttgttt cttttgtaat tcaagtaact gttcgggtgt aacggcggga
    31021 tacccctgcg catcttccgg cacctggtgc atagccggaa tagggaccag cggccctaaa
    31081 aaacgcggtt cacgtttaaa aatataaagg tcagccagcg caccgaaacg ggcggcaaat
    31141 tcgcgaatac gtactgacag catatctttc ggtgacggta cggcataaca ctgagcctga
    31201 atccccatat gcagcgcaat aaataatgct cgctcacagt ggaaacgttg ggtgataata
    31261 atgaaatcat tagtatcgaa aactttgcgt gtacgcacga tggaatccag cgtacgaaag
    31321 cctgcgtaat cgagaacaat atctgatggg tcgacaccag cagcgattaa atctttgcgc
    31381 atggtcatcg gctcgttata actttgcaat gcgttatcgc cgctcagtaa tagataattt
    31441 accttaccgc tgttataggc attaatcgct ccttgaatgc ggtagcgata atactgatta
    31501 ataacgccgg tacgataata ttttgctgtg ccgagcacca cgccgacctg gcggtagggg
    31561 agatcctgca attcgtcgta gatataaggc gcggttttcc agctcatcca gcgatcgagg
    31621 ccgagcacag tcaacagcag caagccgatc aggactaaca ggctgaggaa cacgcgcttt
    31681 aacatgcagg agactcgagc taaggaaaat ttccatcagg ctactttacc cgctggtttt
    31741 gcgcaagaaa cggcaaactg tttgccggga atttcgacac caggagaggg ctggcaagcc
    31801 ggacaaggcg ttcacgccgc atccggcacc aggcttaagc gagaagcacg cggtcgatac
    31861 tgtgacagcc gagagctttc agcgttgcgg aggtggcatc ccactgaatc aacggcgcat
    31921 cggctttttc tgccagaacc gcgcgctgga tatccgggta atcataaccc ttgagattca
    31981 acagaattaa cgccccctgc aacggcggca gagtcgggtt gaatgcggcg ttttcagcgt
    32041 agctgccgct gaaaatacga ccgtctttac attccagcgc gacaccgctt ggtgacttac
    32101 tgtaaggcat gtgcgaacgg tttgccgctg caatcgccgc ctgagaaagc gcatcaccag
    32161 tcagcgcata gccgtgatcc tgctcgtcca tcagcagcgt tttaatctcc agatctttcg
    32221 gcccaaaggc atccggcaga tagtcacgca gcgcgtgtgc ctcgcggccc ggcaaatgaa
    32281 tacgcagatc cagaccgctg ttcagttcat tcataaactg acggcagtga ccacaaggcg
    32341 tatagttaac ggtgatggct gcaagcgctt tttcaccact caaccaagcg tggctgatcg
    32401 cgctttgttc ggcatgaacg gtttgctgca ttgttgcacc aataaattcc atattggcac
    32461 caaaatacca ggttccgctc acaccgcgcg caattgcgcc aacattaaaa ttcgacaatg
    32521 gcgtacgcgc acaggccgcc gccagcggaa gtagtgcgaa tgccagcgcg tcttcgtcca
    32581 gtcccgttgc gctcttcagc gatgagactt gctccccggt caacaaagcg gggaagtact
    32641 tgtctgccag aataggttcc agtgcagatt gcaagttatc cgcaagttgg gcaaaagcgg
    32701 tttgaaaacg tggatgcatg gcgttgcctc ataatcatgt aatgggataa cagtttacga
    32761 acccgttatg gctttatatg tgatctgaat ctcattaatt atgcaacacg tgaaacttac
    32821 atcaaaaatg cgcgacgtat cgcaaatttt agcccattac tgccagaata atcgggaaaa
    32881 ggaacggtgc gatcagcgaa gtaattatcc cgcataacac cagcgctagc gaactaaatg
    32941 caccttcctg ataatccagc tcggcgcagc gcgccgtacc gagggcgtgc gaggcagtcc
    33001 ccattgccag accgcgcgca gctttggtac gaatacgcat cgcattaagc aatgtatggc
    33061 caaataccgc gccgaggatg ccgacgaaaa tcacgcaaac tgcgctgatt gccggaatac
    33121 cgccaatact gccgccaacc gccattgcaa taggcgtggt gactgatttc ggcaggattg
    33181 acgcggcgat ttccggtgaa gcgcccatca acaatgccac ggaagtcccc gtcaccatcg
    33241 caaccacgct gccgataaaa cagatggtga tgatcgattt ccagcgcgcg cggatctggt
    33301 gtagctgctc atataaagga taggccagcg cgacgaccgc cggttgcagc agatcgttta
    33361 gcacttcgct acctttaaag taactgtcgt aagagatgcc ggtcagcatt aaaaaaggaa
    33421 tgatcaccac cattgctacc agcaacgggt taagcagggg aaacttatac cgtgccgcca
    33481 gtttgcgggc ggcaaaaaag acaatcaacg tcagcggtaa tgaccaccag atattcgcca
    33541 tcattcttct gatcctttct gacctacgac tttacgttca ccgtgtacca gttgtgaact
    33601 ccagctcacc accagaaaaa ccaccagcgt actgacagca caggaaacca ctaccgggcc
    33661 aaactgcgcg cggagcaaat caaaatattg catgacgcct acgccaatcg gcacaaacaa
    33721 tagcgccata tagcgaatca gtacgtagca ccccggattg acccatttcg ccggaagaat
    33781 ttgcaaggcc agcaggacaa acaggatcag catcccgatg atgctgcccg gaatggttac
    33841 cggtagcagg gaagcaatga aaatgcctgc atacaggcag gcataaatca ggacgaaagc
    33901 gcgtaaatat tgccagataa tgttcagtgt cttactcata atcaacaccc cagatgagat
    33961 ggattcatca tacaattaat tcgtaaaacg tgctaccgat cacattaaga aatcagcgct
    34021 tattttttcg ctacccggta ttacgcaatg agcagcgaac tcaggcatac atcaagcata
    34081 gagagggatt gcagtttgca gcaatccctc tttgcagacc tttaatccgc tgaccatagg
    34141 cgctaacata gcgcctcatt tttttgcggg tgatgatatg cgtgtgttac tggcaccgat
    34201 ggagggagtg cttgactctc tggtgcgtga attgctgacc gaagttaacg actacgatct
    34261 gtgcatcacc gagtttgtcc gcgtggtgga tcaactgctg ccggtaaaag tctttcatcg
    34321 catttgccct gagctacaaa acgccagccg gacaccatct ggtacgctgg tgcgcgtgca
    34381 gttgttaggt cagttcccac aatggctggc agagaacgcc gcccgtgcgg tggagttagg
    34441 tttctggggc gtggatctca attgcggctg cccgtcgaaa acggttaacg gtagcggcgg
    34501 cggggcgacg ttactcaaag atcctgaact catctaccag ggggcaaaag cgatgcgtga
    34561 agctgtaccg gcgcatttgc ctgtcagcgt gaaagtgcgt ctgggctggg acagcggtga
    34621 gaagaaattt gaaatcgccg atgcggttca acaggctggc gctacggagc tggtggtgca
    34681 tgggcggacg aaagagcagg gttaccgcgc ggagcatatt gactggcagg cgattggcga
    34741 gattcgccag cggctgaata ttccggtgat tgccaacggt gaaatctggg actggcagag
    34801 cgcgcaacaa tgcatggcga tcagcggctg cgacgcagtg atgattggtc gcggggcgct
    34861 caatattccc aacctgagcc gggtggtaaa atataacgaa ccgcgaatgc cgtggccgga
    34921 ggtggttgct ttgctgcaaa aatatacccg tctggaaaag cagggcgata ccgggttata
    34981 tcacgttgcg cggattaaac agtggttgag ttatttgcgt aaagaatacg atgaagcaac
    35041 ggaattattt cagcatgttc gggtgttgaa taactcccct gatattgcaa gggctattca
    35101 ggcaattgat atcgagaaac tctaaaaacc atagtgatgt ttttgacaat gtgttagctc
    35161 tttatcaata ggccgcacgg ttgtttggtg gcgtcaaaat cgccgaggcg ttccctgaag
    35221 gccggggcag cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg
    35281 cccgacccgg tagccgtaag ggataagccg
//
LOCUS       DAJTYH010000033        34957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTYH010000033 DAJTYH010000000
VERSION     DAJTYH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..773)
                     /locus_tag="OO609_001597"
     CDS             complement(<1..773)
                     /locus_tag="OO609_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6633059.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNP"
     gene            865..951
                     /locus_tag="OO609_001598"
                     /pseudo
     CDS             865..951
                     /locus_tag="OO609_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1356..1832)
                     /locus_tag="OO609_001599"
     CDS             complement(1356..1832)
                     /locus_tag="OO609_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6633060.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1891..3180)
                     /gene="bioA"
                     /locus_tag="OO609_001600"
     CDS             complement(1891..3180)
                     /gene="bioA"
                     /locus_tag="OO609_001600"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6633061.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3267..4307
                     /gene="bioB"
                     /locus_tag="OO609_001601"
     CDS             3267..4307
                     /gene="bioB"
                     /locus_tag="OO609_001601"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6633062.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4304..5458
                     /gene="bioF"
                     /locus_tag="OO609_001602"
     CDS             4304..5458
                     /gene="bioF"
                     /locus_tag="OO609_001602"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6633063.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5445..6200
                     /gene="bioC"
                     /locus_tag="OO609_001603"
     CDS             5445..6200
                     /gene="bioC"
                     /locus_tag="OO609_001603"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6633064.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6193..6870
                     /gene="bioD"
                     /locus_tag="OO609_001604"
     CDS             6193..6870
                     /gene="bioD"
                     /locus_tag="OO609_001604"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6633065.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7449..9470
                     /gene="uvrB"
                     /locus_tag="OO609_001605"
     CDS             7449..9470
                     /gene="uvrB"
                     /locus_tag="OO609_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6633066.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9662..10570)
                     /gene="yvcK"
                     /locus_tag="OO609_001606"
     CDS             complement(9662..10570)
                     /gene="yvcK"
                     /locus_tag="OO609_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6633067.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10835..10979
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10967..11956
                     /gene="moaA"
                     /locus_tag="OO609_001607"
     CDS             10967..11956
                     /gene="moaA"
                     /locus_tag="OO609_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6633068.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11978..12490
                     /gene="moaB"
                     /locus_tag="OO609_001608"
     CDS             11978..12490
                     /gene="moaB"
                     /locus_tag="OO609_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6633069.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12493..12978
                     /gene="moaC"
                     /locus_tag="OO609_001609"
     CDS             12493..12978
                     /gene="moaC"
                     /locus_tag="OO609_001609"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6633070.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12971..13216
                     /gene="moaD"
                     /locus_tag="OO609_001610"
     CDS             12971..13216
                     /gene="moaD"
                     /locus_tag="OO609_001610"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6633071.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13218..13670
                     /gene="moaE"
                     /locus_tag="OO609_001611"
     CDS             13218..13670
                     /gene="moaE"
                     /locus_tag="OO609_001611"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6633072.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13807..14511
                     /locus_tag="OO609_001612"
     CDS             13807..14511
                     /locus_tag="OO609_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6633073.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14716..15429
                     /locus_tag="OO609_001613"
     CDS             14716..15429
                     /locus_tag="OO609_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6633074.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15465..16421)
                     /locus_tag="OO609_001614"
     CDS             complement(15465..16421)
                     /locus_tag="OO609_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6633075.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16421..17662)
                     /gene="clsB"
                     /locus_tag="OO609_001615"
     CDS             complement(16421..17662)
                     /gene="clsB"
                     /locus_tag="OO609_001615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6633076.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17659..18420)
                     /locus_tag="OO609_001616"
     CDS             complement(17659..18420)
                     /locus_tag="OO609_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6633077.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18553..18963
                     /locus_tag="OO609_001617"
     CDS             18553..18963
                     /locus_tag="OO609_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6633078.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18925..20031)
                     /locus_tag="OO609_001618"
     CDS             complement(18925..20031)
                     /locus_tag="OO609_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6633079.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20042..21175)
                     /locus_tag="OO609_001619"
     CDS             complement(20042..21175)
                     /locus_tag="OO609_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6633080.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21168..22904)
                     /locus_tag="OO609_001620"
     CDS             complement(21168..22904)
                     /locus_tag="OO609_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6633081.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22897..23892)
                     /gene="hlyD"
                     /locus_tag="OO609_001621"
     CDS             complement(22897..23892)
                     /gene="hlyD"
                     /locus_tag="OO609_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6633082.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23895..24566)
                     /gene="cecR"
                     /locus_tag="OO609_001622"
     CDS             complement(23895..24566)
                     /gene="cecR"
                     /locus_tag="OO609_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6633083.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24795..25982
                     /gene="rhlE"
                     /locus_tag="OO609_001623"
                     /pseudo
     CDS             24795..25982
                     /gene="rhlE"
                     /locus_tag="OO609_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26189..26671)
                     /gene="ybiA"
                     /locus_tag="OO609_001624"
     CDS             complement(26189..26671)
                     /gene="ybiA"
                     /locus_tag="OO609_001624"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6633084.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26791..28941
                     /gene="dinG"
                     /locus_tag="OO609_001625"
     CDS             26791..28941
                     /gene="dinG"
                     /locus_tag="OO609_001625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6633085.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28969..29931
                     /gene="ybiB"
                     /locus_tag="OO609_001626"
     CDS             28969..29931
                     /gene="ybiB"
                     /locus_tag="OO609_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6633086.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30072..31157
                     /gene="hcxB"
                     /locus_tag="OO609_001627"
     CDS             30072..31157
                     /gene="hcxB"
                     /locus_tag="OO609_001627"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6633087.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31386..31646)
                     /locus_tag="OO609_001628"
     CDS             complement(31386..31646)
                     /locus_tag="OO609_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6633088.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31911..32177)
                     /gene="ybiI"
                     /locus_tag="OO609_001629"
     CDS             complement(31911..32177)
                     /gene="ybiI"
                     /locus_tag="OO609_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6633089.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32251..32928)
                     /gene="ybiX"
                     /locus_tag="OO609_001630"
     CDS             complement(32251..32928)
                     /gene="ybiX"
                     /locus_tag="OO609_001630"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6633090.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32970..34957)
                     /locus_tag="OO609_001631"
                     /pseudo
     CDS             complement(32970..34957)
                     /locus_tag="OO609_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8285 a         8965 c         9441 g         8266 t
ORIGIN      
        1 aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat actgtctggc
       61 aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg aagttcgggc
      121 agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg aaggggcggt
      181 agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg taattctggt
      241 aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg aagatcaggt
      301 agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg aagagaaggc
      361 aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ccaggtgagg cggaagagcc
      421 gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg agggagttct
      481 ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg gggcaggtat
      541 gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa agccagacat
      601 attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc cttggcccag
      661 ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa acgggatata
      721 catcctaaac cacaaaagga gcggaaaaaa gaaggattgt gggcaggttt catttattat
      781 tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat tctttaaaaa
      841 aacgcgcctg taaatagtta agaacaggaa gaactgttgg ggatgtggct ggccgaaaat
      901 gaaggggtga agttctggct caatgtgctg actgaactga aaaaacgcgg ttggtcgccc
      961 taccttttca tcaagccagt agtctgccca ccacagcatc atttctctgc gcttatcgag
     1021 atactgagca tggttgtaaa ttcagctaac ccagtgcgtt gacaaaaaat tagcgcaaga
     1081 ggacaaaaaa tcaccttgcg ctaatgctct gtatcaggtc actaatacca tctaagtggt
     1141 tgattcatag tgactggata tgttgtgttt tgcagtatta tgcagtctat tttttaagct
     1201 aaatgtatta taatgcattg atattaatga tttttactgt ttctcggtca gcttttttat
     1261 actaacttga gcgaaacggg aaggtaaaaa gacaaaaagt tgtttttaat acctttaagt
     1321 gataccagat ggcattgcgc catctggcag agtgattaac taaacatcgc ggtaatcgat
     1381 gcgcttgcca gagagtggaa atgaacgtta aacccgacca tcgcgccgct ggctccttca
     1441 tcgacatcaa tacgctctac atccagcgcg tgaacggtaa aaatgtagcg atgggtttcg
     1501 cctttcggcg gcgctgcgcc atcgtatccg gttttaccaa aatcggtacg cgtctgcaaa
     1561 acgccgtctg gcattgctac cagaccagag ccaaaccctt gcggtaatac gcgggtatca
     1621 gcgggtaaat taacaactac ccagtgccac cagccggagc cggttggcgc atccgggtcg
     1681 tagcaggtga caacaaaact tttcgttccc gcaggaacat catcccacgc cagatgcggt
     1741 gaaatattat cgccatcgta acccatgccg ttaaagacat gacgatgcgg caacttatcg
     1801 ccatcgcgca gatcgttact gatgagtttc atgaaccctc ctttcttgtt tgcagaaagt
     1861 gtagccagaa accctcacgc tgacttcccg ttattggcaa aaaaatgttt catcctgtac
     1921 cgcgcggtta accgctgcgg tcagacgctg caactgttgc gggagaataa tatagggcgg
     1981 catcaggtaa atcagtttgc caaaaggccg gatccatacc ccctgttcga caaagaattt
     2041 ttgcagcgcc gccatattca ccggacgagt ggtttcgacc acaccgattg cccccagtac
     2101 gcgcacatcg gcaaccattt cggcatcacg ggctggtgcc agttgctcgc gcagctgcac
     2161 ttcaatagcc gccacctgct gctgccattc gccggattcg ataatcgcta ggctggcgtt
     2221 tgctgccgcg caggccagcg gattgcccat aaaagttggc ccatgcataa agcagccggc
     2281 ttcgccgtta ctgatggttt ctgcaacctc gcgcgtggta agtgtggcgg aaagggtcat
     2341 tgtgccgccg gttaaggctt taccgaggca caaaatgtcc ggcgcgattt ctgcatattc
     2401 acaggcaaac agtttgccgg tacgaccaaa tccggtggcg atctcgtcgg caatcagcaa
     2461 gataccttcg cgatcgcaca ttttgcggat tcgttttaac cattccggat ggtacatgcg
     2521 catcccgcct gcgccctgga caatcggctc aatgatcacc gctgcgattt catgacgatg
     2581 cgccgccatc aggcgggcaa aaccaaccat atcgcgttca tcccattcac catccatgcg
     2641 gctttgcggg gcgggagcaa acaagttttc tggcagatag cctttccaca ggctgtgcat
     2701 tgagttgtcc ggatcgcaca ccgacatcgc gccaaaggta tcgccatgat aaccattgcg
     2761 gaaggtcaga aaacgctggc gcgcttcgcc tttggcttgc cagtactgca acgccatttt
     2821 catcgccact tccaccgcta cggaaccgga gtccgcgaga aaaacgcact ccagcggttg
     2881 cggcgtcatt gccaccagtt tgcggcatag ctcgatggcg ggcgcatggg tgataccgcc
     2941 aaacatcaca tgcgacatgg catcaatttg cgtcttcatc gccgcattaa gctgcgggtg
     3001 attgtagccg tggatcgccg cccaccagga cgacataccg tcaaccaggc gtctgccgtc
     3061 agacaaaatt agctcgcaac cttcggcgct cgccaccgga taaaccggca gaggggaggt
     3121 catggatgtg tatgggtgcc agatatggcg ttggtcaaag gcaagatcgt ccgttgtcat
     3181 aatcgacttg taaaccaaat tgaaaagatt taggtttaca agcctacacc gaattaacaa
     3241 caaaaaacac gttttggaga agccccatgg ctcaccgccc acgctggaca ttgtcgcaag
     3301 tcacagaatt atttgaaaaa ccgttgctgg atctgctgtt tgaagcgcag caggtgcatc
     3361 gccagcattt cgaacctcgt caggtgcagg tcagcacgtt gctgtcgatt aagaccggag
     3421 cttgtccgga agattgcaaa tactgcccgc aaagctcgcg ctacaaaacc gggctggaag
     3481 ccgagcggtt gatggaagtt gaacaggtgc tggagtcggc gcgcaaagcg aaagcggcag
     3541 gatcgacgcg cttctgtatg ggcgcggcat ggaagaatcc ccacgaacgc gatatgccgt
     3601 acctggaaca aatggtgcag ggggtaaaag cgatggggct ggaggcgtgt atgacgctgg
     3661 gcacgttgag tgaatctcag gcgcagcgcc tcgcgaacgc cgggctggat tactacaacc
     3721 acaacctgga cacctcgccg gagttttacg gcaatatcat caccacacgc acttatcagg
     3781 aacgcctcga tacgctggaa aaagtgcgcg atgccgggat caaagtctgc tcaggtggca
     3841 ttgtgggctt aggcgaaacg gtaaaagatc gcgccggatt attgctgcaa ctggcaaacc
     3901 tgccgacgcc gccggaaagc gtaccaatca acatgctggt gaaggtgaaa ggcacgccgc
     3961 ttgccgataa cgatgacgtt gatgcctttg attttattcg caccattgcc gtagcgcgga
     4021 tcatgatgcc gacctcttac gtgcgccttt ctgccggacg cgagcagatg aacgaacaga
     4081 ctcaggcgat gtgctttatg gcaggcgcaa actcgatttt ctacggttgc aaactgctga
     4141 ccacgccgaa tccggaagaa gataaagacc tgcaactgtt ccgcaaactg gggctaaatc
     4201 cgcagcaaac tgccgtgctg gcaggggata acgaacaaca gcaacgtctt ggacaggcgc
     4261 tgatgacccc ggacaccgac gaatattaca acgcggcagc attatgagct ggcaggagaa
     4321 aatcaacgcg gcgctcgatg cgcggcgtgc tgccgatgcc ctgcgtcgcc gttatccggt
     4381 ggcgcaagga gccggacgct ggctggtggc ggatgatcgc cagtatctga acttttccag
     4441 taacgattat ttaggtttaa gccatcatcc gcaaattatc cgtgcctgga agcagggggc
     4501 ggagcaattt ggcatcggta gcggcggctc cggtcacgtc agcggttata gcgtggtgca
     4561 tcaggcgctg gaagaagaac tggccgagtg gcttggctat tcgcgggcgc tgctgtttat
     4621 ctctggtttt gccgcgaatc aggcagtcat tgccgcgatg atggcgaaag aggaccgtat
     4681 tgttgccgac cggcttagcc atgcctcatt gctggaggct gccagtttaa gcccgtcgca
     4741 gcttcgccgt tttactcata acgatgtcgc tcatctggcg cgactgcttg cttccccctg
     4801 tccggggcag caactggtgg tgacagaagg cgtgttcagc atggacggcg atagcgcgcc
     4861 actggcggaa atccagcagg taacgcaaca gcacaatggc tggttgatgg tcgatgatgc
     4921 ccacggcacg ggcgttatcg gggagcaggg gcgcggcagc tgctggctgc aaaaggtaaa
     4981 accagaattg ctggtagtga cttttggcaa aggatttggc gtcagtgggg ctgcggtgct
     5041 ttgctcaagt acggtggcgg attatctgct gcaattcgcc cgccacctta tctacagcac
     5101 cagtatgccg cccgctcagg cgcaggcatt acgtgcgtcg ctggcggtca ttcgccgtga
     5161 tgagggggat gcacggcgcg aaaaactggt gtcactcatt gcgcgttttc gtgccggagt
     5221 acaggatttg ccgtttacgc tggctgattc atgcagcgcc atccagccat tgatcgtcgg
     5281 tgataacagc cgtgcgttac aactggcaga aaaactgcgc cagcaaggct gctgggtcac
     5341 ggcgattcgc ccgccaaccg tacccgctgg tactgcgcga ctgcgcttaa cgctaaccgc
     5401 tgcgcatgaa atgcaggata tcgaccgtct gctggaggtg ctgcatggca acggttaata
     5461 aacaagccat tgcagcggca tttggtcggg cagccgcaca ctatgagcaa catgcagatc
     5521 tacagcgcca gagtgctgac gccttactgg caatgcttcc acagcgtaaa tacacccacg
     5581 tactggacgc gggttgtgga cctggctgga tgagccgcca ctggcgggaa cgtcacgcgc
     5641 aggtgacggc gttagatctc tcgccgccaa tgcttgcgca agcccgccat aaagatgccg
     5701 cagatcatta tctggcagga gatatcgaat ccctgccgtt agcgactgcg acgttcgatc
     5761 ttgcatggag caatctcgcg gtgcagtggt gcggtaattt atccacggca ctccgcgagc
     5821 tgtatcgggt ggtgcgcccc ggaggcgtgg tcgcgtttac cacgctggtg cagggatcgt
     5881 tacccgaact gcatcaggcg cggcaggcgg tggacgagcg tccgcatgct aatcgctttt
     5941 taccgccaga tgaaatcgaa gagtcgctga acggcgtgcg ttatcaacat catattcagc
     6001 tcatcacact atggtttgat gatgcgctca gtgccatgcg ttcgctgaaa ggcatcggtg
     6061 ctacgcatct tcatgaaggg cgcgacccgc gaatattaac gcgttcgcag ttgcagcgat
     6121 tgcaactggc ctggccgcaa caacaggggc gatatcctct gacgtatcat ctttttttgg
     6181 gagtgattgc tcgtgagtaa acgttatttt gtcaccggaa cggataccga agtggggaaa
     6241 accgtcgcca gttgtgcact tttacaagcc gcgaaggcag taggttatcg gacggcaggt
     6301 tataaaccgg tcgcctctgg cagcgaaaag accccggaag gcttacgtaa tagtgacgcg
     6361 ctggcgttac agcacaacag cagcctgcag ctggattacg caacggtaaa tccttacacc
     6421 ttcgcagaat ccacctcgcc gcacatcatc agcgcccaag agggcaggcc gatagaatca
     6481 ttggtaatga gcgccggatt acgcgcgctt gaacaacagg ctgactgggt gttagtggaa
     6541 ggtgctggcg gctggtttac gccgctttct gacactttca cttttgcaga ttgggtaaca
     6601 caggaacaac tgccggtgat actggtagtt ggtgtgaaac tcggctgtat taatcacgcg
     6661 atgttgactg cgcaggcaat acaacacgcc ggactgactc tggcgggttg ggtggcgaac
     6721 gatgttacgc ctccgggaaa acgtcacgct gaatatatga ccacgctcac ccgcatgatt
     6781 cccgcgccgc tgctgggaga gatcccctgg cttgcagaaa atccagaaaa tgcggcaacc
     6841 ggaaagtaca taaaccttgc cttgttgtag ccattctgta tttggttaaa ttgcgagcga
     6901 gatcgcgtct tcgattgact gcaatttaac caattaaatt ctaaaataat cacgaaaaaa
     6961 attttacttc cgcctcatgc ggcgaatgtg ggaattgccc aggcggcggg ggataggggc
     7021 tggagacagt tatccactat tcctgtggat aaccatgtgt attagagtta gaaaacacga
     7081 ggcaagcgag agaatacgcg gcttgcacgc gaattggcgt taaagacggc tcaaagaaat
     7141 atcttttatt ttttaactgg ttagataaat gcaatggcag tcactgaaca ggcatctctt
     7201 gccataaaac tgtcatcact catcttgaca aatgttaaaa aagccgttgc tttggggata
     7261 acccggtaag gctggagttt tatctcgcca cagagtaaat tttgctcatg attgacagcg
     7321 gagtttacgc tgtatcagaa atattatggt gatgaactgt ttttttatcc agtataattt
     7381 gttggcataa ttaagtacga cgagtaaaat tacatacctg cccgcccaac tccttcaggt
     7441 agcgactcat gagtaaaccg ttcaaactga attccgcttt taaaccttct ggcgatcagc
     7501 cagaggcgat tcgacgtctc gaagaggggc tggaagatgg cctggcgcac cagacgttac
     7561 ttggcgtgac tggctccggg aaaaccttca ccattgccaa tgtcattgct gaccttcagc
     7621 gcccaaccat ggtacttgcg cccaacaaaa cgctggcggc ccagctgtat ggcgaaatga
     7681 aagagttctt cccggaaaac gcggtggagt atttcgtctc ctactacgac tactatcagc
     7741 cggaagccta tgtaccgagt tccgacacct tcattgagaa agatgcctcg gttaacgaac
     7801 atattgagca gatgcgtttg tccgccacca aagcgatgct ggagcggcgt gatgtgattg
     7861 tagtggcgtc tgtttccgcg atttatggtc tgggcgatcc tgatttatat ctcaagatga
     7921 tgctccatct cacggtcggt atgattatcg atcagcgcgc gattctgcgc cgactggcgg
     7981 agctgcaata cgctcgtaat gatcaagcat tccagcgtgg tactttccgc gttcgtggcg
     8041 aggtgataga tatcttcccg gcagaatcgg atgacattgc acttcgcgtg gaactgtttg
     8101 acgaggaagt ggaacgattg tcgttatttg acccgctgac cgggcagatt gtttccacta
     8161 ttccacgttt taccatctac ccgaaaacgc actacgtcac accgcgcgag cgcatcgtac
     8221 aggcgatgga ggagatcaaa gaagaactgg ccgccagacg caaagtgctg ttggaaaaca
     8281 acaaactgct ggaagagcag cggctgaccc agcgtaccca gtttgatctg gagatgatgg
     8341 acgagctggg ctactgttcg gggattgaaa actactcgcg cttcctctcc ggtcgtggac
     8401 cgggtgagcc accgccgacg ctgtttgatt acctgcctgc cgatgggctg ctggtcgtcg
     8461 atgaatctca cgtcaccatt ccacaaattg gcggcatgta tcgcggtgac cgggcgcgta
     8521 aagagacact ggtggagtac ggcttccgcc tgccatcagc gctggataac cgtccgctta
     8581 agtttgaaga gttcgaagca ttagcgccgc aaaccatcta tgtttcggcg acgccgggta
     8641 attacgagct ggaaaaatcc ggcggcgatg tggtggatca ggtggtgcgt ccaaccggat
     8701 tgcttgaccc gattatcgaa gtgcggccgg tggcgacaca ggttgatgat cttctttcgg
     8761 agattcgtca gcgagcggca attaacgaac gcgtgctggt caccacactg accaagcgaa
     8821 tggcggaaga tcttaccgaa tatctcgaag aacacggtga gcgcgtgcgt tatcttcact
     8881 cagatatcga caccgtcgag cgtatggaga ttatccgcga cttgcgtctg ggtgagttcg
     8941 acgtattggt agggatcaac ttactgcgtg aaggtctgga tatgccggaa gtgtcgctgg
     9001 tggcgatcct cgacgctgac aaagaaggct tcctgcgttc cgaacgttcg ttgatccaga
     9061 ccattggtcg tgcggcacgt aacgttaacg gtaaagcgat tctctacggc gataggagca
     9121 ctccatcaat ggcgaaagcg attggcgaaa ccgaacgtcg ccgtgagaaa cagcagaagt
     9181 acaacgagga acacggcatt acgccgcaag gcttgaacaa gaaagtggtc gatatcctgg
     9241 cgctggggca gaacattgcc aaaaccaaag cgaagggcag aggaaaatcg cgcccgattg
     9301 ttgagccgga taatgtgccg atggatatgt tgccgaaagc gttgcagcag aaaatccatg
     9361 aactggaagg gctgatgatg caacacgcgc agaatctgga gttcgaagaa gcggcacaaa
     9421 ttcgtgacca gttgcatcag ttgcgtgagt tgtttattgc cgcatcgtaa caggatagcg
     9481 aagaagactg atgacaaacg gaaaacagtc tgatgcgcta cgcttatcag gcctacattt
     9541 tctccgcaat atattgaatt tgcgcggttt gtaggccgga taaggcgttc acgccgcatc
     9601 cggcataaac aacgcgcact ttgtcttcaa taggaagccg gaattttcct tcggatttcc
     9661 gttaacctaa agcctgtaac gccttttcca gcgcgttatg taacaactgg cggtcatgac
     9721 gatacggaat atcgctggcc tccagtacct cctggatcac aatccgctct ttcaccgccg
     9781 agacatccac ttttggcccg acgatgaccg catcaatgac ctttttacca acatactgct
     9841 ccataattgc cagcttgctt tccagcttca aattagccgc aggtaaactc aactcacgcc
     9901 ccagattgcc gatataaacc atcggcgctg gcgtgcggcg taatgcctgg gcgatttcct
     9961 tcagcagcag aattggcatc aggctggtat aaaaactgcc aggcccaata atgatgagat
    10021 ccgcttcatt gatagcgtga accgcctcac gcgttgcggg tacattaggc gttaacaata
    10081 actcttgaat cggcgtagtt aactggtcga tattgacctc gccgtaaact tcatgcccct
    10141 gatcgtcaat cgccatcaga tcaacaggat gctctgacat tggaatcaaa tgcgtatcca
    10201 ctttcagcag attacgaatt aaattgatgg cttccagagg ccgcacgcta aggtgatcca
    10261 gcgcctttaa catcaagttt ccgagattat gaccggaaag ttcgccattg ccgccaaaac
    10321 ggtattcaaa catcgcggag gcgacgttcg gttccgttat cagctggttg aggcagttgc
    10381 gcatatcgcc ccaagcaatg ccgccttctg aacggcgaat acgccccgtc gagccaccat
    10441 tatccgtggt ggtgacgata cccgttaaac gagaacccaa agacgaaagt gatgagagaa
    10501 cgcgtcccag tccatgccct ccgccgagag caacgacacg atcaagatca gccagcgtac
    10561 gattgcgcat atagtttcct ggagtcagat tatccgcgct acagtagcgc aaattgcggg
    10621 gaaacagcaa ttaacctgcc aatattgagg atacaaacta ttttcttcta tctctaaagg
    10681 acgatgcacg ctatgcctcc ctgatgatgt atatcaaagt aaaaccgcca ttttccctta
    10741 ttctgtagcg aaatagcacg atcatgacgc tatatacatg attacatagc gaaagtgtgg
    10801 atggtaaaaa tctcatttac acgctagtat cggcttaacc actaaacact ctagcctctg
    10861 cacctgggtc aactgatacg gtgctttggc cgtgacaatg ctcgtaaaga ttgccaccag
    10921 ggcgaaggaa gaaatgactt cgcctcccgt atctggaaag gtgtacatgg cttcacaact
    10981 gactgatgta tttgcgcgta agttttacta cttgcgcctg tcgattaccg atgtgtgtaa
    11041 ctttcgttgc acctactgcc tgccggatgg ctacaaaccg agcggcgtca ccaataaagg
    11101 ctttcttacc gtcgatgaaa ttcgccgggt tacgcgcgcc ttcgccagtc tgggcaccga
    11161 aaaagtgcgc ctgacaggag gagagccgtc tttacgccgc gactttaccg atatcatcgc
    11221 cgctgtgcgg gaaaacgacg ctatccgcca gattgcggtc accaccaatg gttaccgtct
    11281 ggaacgcgat gtggcgaact ggcgcgatgc gggacttact ggcattaacg tcagtgtcga
    11341 cagtttggac gcccgccagt ttcacgctat taccgggcag gataaattca accaggtcat
    11401 ggcagggatt gatgctgcat ttgaggccgg ttttgagaag gtcaaagtca ataccgtgct
    11461 gatgcgtgat gttaatcatc accagctcga cacctttctg aactggatcc agcatcgccc
    11521 tatccagctg cgtttcatcg aactgatgga aacgggcgag ggcagcgagc tcttccgtaa
    11581 gcatcacatc tctggtcagg ttctgcgtga cgagctactg cgtcgcggct ggatccacca
    11641 attacgtcaa cgcagcgacg gtcccgcgca agtcttttgc catccagatt acgccggaga
    11701 gattggcctt atcatgccgt atgaaaaaga cttctgcgcc acttgcaacc gcctgcgcgt
    11761 ttcctccatt ggtaaactcc atctctgcct gtttggtgaa ggcggcgtta acctgcgcga
    11821 tctgctggaa ggcgataccc agcaacaggc gctggaagcg cgtatttcag cggcgctgcg
    11881 ggagaagaaa cagacccatt tcctgcatca aaacaacacc ggtattacgc aaaacttatc
    11941 gtacattggc ggctaaaacg tcaaaaggag agatcagatg agtcaggtaa gcactgaatt
    12001 tatcccgacc cgtattgcta ttcttacggt ttctaatcgt cgcggtgaag aagacgatac
    12061 ctccggtcac tatctgcgcg attcggcgca agaagcgggc catcacgttg tcgatgaagc
    12121 catagtgaaa gaaaaccgct acgctattcg cgctcaggta tctgcgtgga tcgccagcga
    12181 cgatgtacaa gtggtattga ttacgggtgg tactggcctg acggaaggtg atcaggctcc
    12241 cgaagcgctg ctgccgttgt tcgaccgtga ggttgaaggt tttggtgaag tgttccgtat
    12301 gttgtcgttt gaagagattg gcacttccac gttgcaatct cgtgcggtag cgggcgtcgc
    12361 taacaaaacg ctgattttcg ccatgccggg ttcgaccaaa gcgtgccgta ccgcatggga
    12421 aaatatcatc gcgccgcagc tggatgcccg tacgcgtccg tgtaatttcc atccacattt
    12481 gaagaaataa gtatgtcgca actgacccat atcaacgccg ctggcgaagc gcacatggtg
    12541 gatgtctccg ccaaagcgga aaccgtgcgt gaagcgcggg cggaagcctt tgtcaccatg
    12601 cgcagcgaga cgctggcgat gattattgat ggtcgccacc acaaaggcga cgtatttgcc
    12661 actgcgcgta ttgccggtat tcaggcggca aaacgcactt gggatctgat cccgctgtgt
    12721 cacccgctga tgctcagcaa agttgaagtc aatttacagg ccgagccgga gcacaatcgg
    12781 gtgcgtatag aaaccttatg ccgcctgacc gggaaaaccg gtgtcgaaat ggaagcgtta
    12841 accgcggcct ccgtggcggc gctgaccatt tatgacatgt gcaaagcggt gcaaaaagat
    12901 atggtgattg gtccggtacg tttgctggcg aagagcggcg gcaagtcggg tgactttaag
    12961 gtggaagcgg atgattaaag ttcttttttt cgcccaggtg cgcgagttgg tgggaacaga
    13021 tgcaaccgaa gtggctgcgg attacccaac cgttgaagcg ttacgccagc acctggctgc
    13081 gcagagcgat cgctgggcgc tggcgctgga agatggcaaa ttgctggctg ccgtcaacca
    13141 gacgctggtg agttttgacc atccgctgac tgacggcgac gaagtagctt tcttcccgcc
    13201 ggtaaccgga ggttaagatg gcagaaacca aaattgttgt cggcccgcag ccgttcagcg
    13261 taggagaaga gtacccgtgg ctggcggagc gtgacgaaga cggtgcggta gtcaccttca
    13321 ccggaaaggt gcgcaaccat aacctgggcg acagcgtcaa agcattaacc ctcgaacact
    13381 atccggggat gactgaaaaa gcactggcag aaattgttga tgaagcgcgt aaccgctggc
    13441 cgctggggcg cgtcacggtg attcaccgca tcggggaatt atggccgggc gatgaaatcg
    13501 tttttgtcgg tgtcaccagt gcgcatcgca gcagtgcgtt tgaagccggg cagtttatta
    13561 tggattatct caaaacccgc gcaccgttct ggaagcgcga agccacgccg gaaggcgacc
    13621 gctgggttga agcccgggag agcgatctgc aggcggcaaa acgctggtag ttttttgtta
    13681 gccggataag gcaccgctgc gtccggcaat ttcaccgctc agtacatgcg tttacgctgc
    13741 gtcgatgcac agcctcatca ttttgcagta tccttaagat attccttata tcttcaggag
    13801 atcgtcatgg acagattccc acgttctgat tcaatcgtac aaccctgggc tggcttgcaa
    13861 acttatatgg cgcaagtcta tggctggatg accgttggct tgttgctgac cgcatttgtt
    13921 gcctggtatg cggctaattc cgcggccgtg atggagctgt tgttcactaa ccgtgtcttt
    13981 ttaatcggtc tgatcatcgc gcaattagca ttggttattg tgttatcagc gatgattcaa
    14041 aagctgagcg caggtgtaac gacgatgctc tttatgcttt attcggcgct gacgggtctt
    14101 acgctttcca gtatattcat tgtctatacc gctgcttcta tcgccagtac tttcgtcgtt
    14161 actgccggga tgttcggcgc aatgagcctg tacggttaca ccacgaagcg cgatttaagt
    14221 ggctttggca atatgctgtt tatggcgtta atcggcattg tgctggcatc gctggtcaac
    14281 ttctggctga aaagcgaagc attgatgtgg gcagttacct acatcggcgt gattgtcttt
    14341 gtcggattga cggcgtatga cactcagaaa ttgaaaaata tgggtgagca gattgatacc
    14401 cgcgacacgt cgaacctgcg caaatattcc attcttggtg cgttaacctt gtatctggac
    14461 ttcatcaacc tgttcctgat gttgttacgg atcttcggca accgccgtta attctttttc
    14521 accatcacta tcccggccat gtgtcgggat ttttttctta ctaaaaacga cacttaacaa
    14581 gtaccaggta taaaatatgc cttatgagcg agcggaatcg ttttccgtat ccataaatac
    14641 aaaagtattt tgtatgcgtg gttttaaatt tattgatggt gaatattaat gttttttcta
    14701 aggacaacta aaagaatgga atcatactcg caaaacagca ataaattaga ttttcagcac
    14761 gaggccagga tattaaacgg tatatggctc attaccgctt taggtttggt ggcaaccgca
    14821 ggactagcct ggggagctaa gtatctcgaa attacggcaa ccaaatatga ttcaccacca
    14881 atgtatgtcg ccatagggtt attattactt tgtatgtatg gcttaagtaa ggatatcaac
    14941 aagataaatg ccgccatcgc gggcgtaata tatctgtttt tactctcttt ggtggcgatt
    15001 gtcgttgcaa gtttagttcc tgtatatgcc attatcatcg tgttcagcac tgcgggcgcg
    15061 atgtttttaa tcagtatgct ggccggttta ttatttaatg ttgatcctgg ttctcaccgt
    15121 tttatcatta tgatgacgtt gacagggttg gccctggtaa tcatcgtgaa tgcggcatta
    15181 atgagtgaac ggcccatttg ggtaataagt tgcttaatga ttgtgttatg gccaggcatt
    15241 atctcgcatg gacgaaataa gctccttgaa ctggcaggaa aatgccatag tgaagagttg
    15301 tggagtccgg ttcgttgcgc ttttacaggt gcattaacac tctattacta ttttatcggc
    15361 ttctttggga tacttgccgc gatagctata acgcttgtct ggcaaaggca tacgcgtttt
    15421 tttcattagt cgtaaatgca gatactaaag aacttaacta aacttcacat cgccgcttca
    15481 tttttcgccc gtagcttctt cgcttggctt tccagtagca ggtaacagat cagcgccagc
    15541 agcagcggga taaagtaata caggacgcga taagcgagca gggcggcgat aatgctccct
    15601 ttcgaggtat gctccccagc cagtagtgcg ataaacaccg cttccagcac accgatcccc
    15661 gccggaatat gcacgatgac gccagcaata ctactaacca gtaacacgcc cagtacaaag
    15721 aaatagttca cgctttgacc aagtaacagc cagataatcg cccccattac catccagtta
    15781 acgctggaaa tcagcatctg agcgagggcg aatttccatg aaggcagcac cagcttttgc
    15841 cctttgatgg tcatatggcg gtgcttcgcg aaagcgcaaa accacaaata aaccgcgata
    15901 atcatcagta agccaatgcc gagaatacgc agcgtagttt gatcgacata ccagtaatcc
    15961 ggcaactcca ccacgcctgc ggtaaagata atccctgcca gtaaaatata gcccagccag
    16021 ttggtggtaa tactgagcga ggaaatccgc gtaatagtgc tgcccggtaa ccctagtcgg
    16081 gagtacaaac gatagcgcat accaatgccg ccgacccagg tactgagcgt caggttgaag
    16141 gcgtagcaga taaacgacac cagcatcacc tggcgcttcg ccagtttgtg accgcagtaa
    16201 aagcgggcga gcaggtcata gcagccgtaa atcagatagc tgacgaccac cagcccgacc
    16261 gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc ttcccagtcc
    16321 acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag ccaggtgagg
    16381 atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcaggggttt acccccgtgt
    16441 tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggggga tcaacctgcg
    16501 tcagacgtgg tgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg cgtaaaaagt
    16561 ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc atggtttcat
    16621 ccacctgctg acaatctgcg gcaataatgc cgttcagatt atcgcgcagc gtctggttaa
    16681 aatgacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg agcggatcga
    16741 gattactgga ccctactgtc gcccagtgat cgtccatcaa tgccactttg ccgtggagcg
    16801 ggcggcggcg gtactcaaaa acctgaacgc cgcctttaac cagatagtta tacagcaagc
    16861 gcgcaccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt ttgatccgca
    16921 ccccgcgccg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg aagaagtagg
    16981 cgttggcgat aatcacttcc cgctgcgcct gagtgagcat tttcagataa tggcgttcaa
    17041 tatcatcgcg atgttcttcg ttatcgcgcc agaccagcaa tacctgcgct tctcccggct
    17101 ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg ctctgtccag
    17161 gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca aggcgtaccg
    17221 cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag taattcagcc
    17281 cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc cggcgaaaca
    17341 cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac actacgccag
    17401 ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag ccatccagca
    17461 agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc agctgtttgc
    17521 cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt gcctcgccaa
    17581 tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg atcttattgc
    17641 cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat gatcagaaag
    17701 gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat ttttgacgta
    17761 gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg ggcgtccgtg
    17821 ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat tagctttttg
    17881 ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta gctcattcac
    17941 ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc ccagatgtac
    18001 gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt agagcacgcc
    18061 gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa tgggataacg
    18121 cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac cgtaggcaaa
    18181 atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc agttttccac
    18241 atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa tatcggcgct
    18301 gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa acgcggtaaa
    18361 gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt gatcgggcat
    18421 aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc acaaaaaggt
    18481 gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga taaattcttc
    18541 aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct aagttgctgg
    18601 cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat gagtaagact
    18661 ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt gatgatgctg
    18721 gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct ggaagagctg
    18781 accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc ccgcgtactg
    18841 gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc ggcggtagtg
    18901 tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg cgtacgccgc
    18961 tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca caatatccag
    19021 actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa tagggttaat
    19081 ccacgtcaga ttttgcagcc acactggcat gttttccacc ggcgaaacgt aaccggaaag
    19141 gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt gtgtcgagca
    19201 gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca ccatcgtaaa
    19261 gtagaacagc gctagcgatc cggcgaaggg gatttgatac gcccagatac caatcgccag
    19321 cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc cgatgaagat
    19381 ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt cacgttcgcg
    19441 ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg cgatcagcga
    19501 cggcaccaca aaccatttgt agtcgagatt cgggttatac cagttgcgta ccaccagctc
    19561 gctgttgttg ggtttcggtt ttccttccag cagctcctgc tgataatttt tgacgatctg
    19621 ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga ggatcaactg
    19681 caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga aacgcaccag
    19741 tagtaacgcc ttttgtgtgt cgatggtcgg gcggatctcc tgtgggcttt tcagcagcag
    19801 cacatgtgta aaggcgctgg cgcgggcaaa acgttgagta agttccaccg aatgctcgcc
    19861 gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg cggcgaacgg
    19921 gaacaggatc acctgaatta gcacgggtaa aatcagaatc gcgcgggttt gtggttcgcg
    19981 cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca tgctcttctc
    20041 cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc accgccgaag
    20101 cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg aggaacaggc
    20161 tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg cggatcaccg
    20221 cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc agaaaagcgg
    20281 cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa atcagcagcc
    20341 ccattcccag ggtactgagt aaaaacaggc tggagataaa aaacagaatc agcagcgaac
    20401 cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcaac atcgccagca
    20461 tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg gtaatctccg
    20521 tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc agcgaggtga
    20581 gaatcgcgcc gatgaccgtc atgataatgg tcaccgcacc cgggataata aagtgctggc
    20641 ttatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt tcaaaagtct
    20701 gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg acatacccct
    20761 gcacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc ggtgcggtgg
    20821 cgttggcgcg ctccatctgt tcggcgaaat ccacgggaat aaccaccaga ccgcgaattt
    20881 tccccgactg cattttggcg atcagttcct gacggttatc gctgatggtg gcgtcgatgt
    20941 agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc tgttccagca
    21001 aaatcccgac ccgcagcttg ctggagtcga ggttaatgcc gtaaccaaaa ataaacagca
    21061 gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg atctgccgcg
    21121 tctctttgac gcacagcgcc cgcacgcgat gccaggaaag gatcgggtta ctcattgcta
    21181 tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt gggatcgggt
    21241 tgctcatcgt tagctgactg tgctttcaaa tcgtccggcg taccgctggc gattaatttc
    21301 ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa gtgggtggtg
    21361 accatcaccg tgacgccttt ctctaccatg ctgttgatgt gcagccaaaa ttcacggcgg
    21421 gtgagggggt caacgccgga agtcggttcg tcgagaaaga gaatatccgg ttcatgcatc
    21481 agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc ggtggcgtgt
    21541 gaggcaatac ttttcaggcc aaatgcctcg ctcatacggg agattttttc gttctgcgcc
    21601 cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac cgtcaggttg
    21661 ccgtagagcg aaaatttttg cgccatatag ccgagatgct ggcgcgcttt accggaactc
    21721 tctttcagat ccatccccag caccagcgcc tggccggaag tcggcaccag caaaccgcac
    21781 atcatcttaa aggtggtcga tttacccgcg ccgtttggcc ccagcaaacc aaaaatctcc
    21841 ccacgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa tttcttggtc
    21901 agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg caatattgca
    21961 cccagcggcg attctgaggt tccggcaccg cccagcaaat caataaacgc atcttcaaaa
    22021 cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg aatatcgtct
    22081 ggtgtggcct cttttttgag gatcagacgt accgatttcc cctgaatcat gccatcgctg
    22141 acctgcggca gtttcaaggc gcgttgcaac agtttgcggt tgccctcgtg tggactggtc
    22201 atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttctcc ctgatacagc
    22261 aactcgcctt cgttcatcag taacacgtca cggcactgct cggcttcgtc gagatacgag
    22321 gtactccaga ggattaacat cccttcgccc gccagctcat gcaccatctg ccacagttcg
    22381 cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac tttcggttcg
    22441 cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag cttgcccgcc
    22501 aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa agtttgctta
    22561 cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc catcaccgtg
    22621 agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg cagcgcgccg
    22681 tcgtttttga tcggatcaaa gccaatcacc gtggcactgc cgctgtcggg tttcagtaat
    22741 cccgccaaca tccgcatcag tgtggtttta cctgcaccgt ccggccccac caaccccgtc
    22801 acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc catgcccgga
    22861 aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg cctcgtcacc
    22921 gaattgtacc gtcactggca ttccctggcg taacgcatca tcggcgtcgg tcaccacaat
    22981 acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt tcggggtaaa
    23041 ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg ggcgaccatc
    23101 ggtataaagc agcactttgc gccccggctg ggcctggtca agattccgtt catcaacata
    23161 agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac cttcattgag
    23221 aaccgtgcct ggctctaccg cgcgcgttaa cagcgtgcca tcagacggtg ctatcaacgt
    23281 tgagtcctgt aaattcagct ccgcctgcgc cagttgcgcc tgcgcctgtt cgaggctggc
    23341 tttcgcctga gcgatgtcct gttcacggtt accggaacgg tactgacgca atttatcctg
    23401 tgctgatttc agcgttgcct gtgcctggtc gcgcgaggag cgggcatttt ccaggtcatt
    23461 tgccgaaatg gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt tctgcgcata
    23521 gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt cttcatcgcg
    23581 atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg ctttcgcctg
    23641 catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc ccgctttgat
    23701 agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga aactaagatt
    23761 caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc ggctttgata
    23821 ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtc cgatcacgac
    23881 aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag gtcgatatga
    23941 caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt ccagccggta
    24001 cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat caacgcatgg
    24061 gtatgaagga tcatgcgggt gtcattagcg tcgcagccgg tccaggcggc aatcaggcgt
    24121 gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta ggctgccgtg
    24181 ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc ctgggtgagc
    24241 agcttaatca tgttcctgca ggcgcgaagg atcagttcac ggatggcagc ccgatcaggc
    24301 tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg ctcgccaata
    24361 aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc gaagtagtag
    24421 gtgatggcag cgatattctg cccggcctgg gcggctatct cgcgggtggt ggcgttcatt
    24481 ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc ctgttcaccc
    24541 ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat gattgattaa
    24601 gattatgact ccatatggga gttgtccagt atggctaaga attttagcta cgccagtcac
    24661 agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctccctc atgacattgt
    24721 ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa cggtcggggc
    24781 ggttcggagt agttatgtct ttcgattctt tgggtttaag ccctgatatc ctgcgcgccg
    24841 ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc cctgctgtgc
    24901 tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca gcgggcttta
    24961 cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg cgtcgtccgg
    25021 tacgtgcgct cattcttacc ccgacccgtg aactggcggc acagattggc gaaaacgtcc
    25081 gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggcggt gtcagcatta
    25141 acccacaaat gatgaagttg cgcggcggcg ttgatgtgct ggtggcaacg ccgggacgtt
    25201 tgctggacct ggaacatcaa aatgcagtga agctggatca ggttgaaatc ctcgtcctcg
    25261 atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc gtgttaacaa
    25321 aactgccagc gaaacgccag aacctgctgt tctctgcgac cttctctgac gatattaaag
    25381 ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc cgcaataccg
    25441 cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa cgcgaattgc
    25501 tgtcgcacat gattggtaaa gggaactggc agcaggtgct ggtgtttacc cgtaccaaac
    25561 acggcgctaa ccatctggct gagcagctca ataaagatgg catccgtagt gtggcgatcc
    25621 acgggaataa atcgcaaggt gcgcgtaccc gtgcgctggc tgattttaaa tcgggtgaca
    25681 ttcgcgtact ggtggcgacc gatatcgctg cgcgcggtct ggatatcgaa gagttgccgc
    25741 acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccaccgt atcgggcgta
    25801 ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat gaacacaaac
    25861 tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg attccgggct
    25921 atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag caacgtggcg
    25981 gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacgt
    26041 gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg tttacgccgc
    26101 atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa aaatttatct
    26161 gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt tcgcgcaact
    26221 ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct ccccagtaag
    26281 catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag agcacacgca
    26341 gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt tctttaaccg
    26401 actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg gcgaccatgg
    26461 gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt tgtgcctgaa
    26521 aatagtgttc ggaggtgggc caggtattcc cgtcaacctt gatgggcctg gcggcaaagt
    26581 tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg tcttgcatga
    26641 cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt ggttattgcg
    26701 agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg ccacaatatt
    26761 ggctgtttat acagtatttc aggttttctc atggcattaa ccgccgcgct taaagcgcaa
    26821 attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc ccgtgcgccg
    26881 cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg gcggcatctg
    26941 gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc cggcatcgcc
    27001 attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc gttgcaggat
    27061 cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct taaattcact
    27121 gccgctttcg ggcgtgggcg ctacgtttgt ccacgtaatc tgacggcgct cgccagcact
    27181 gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc aaataatcag
    27241 gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa atgggatggt
    27301 ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt gagtaccgac
    27361 aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt ttttgttact
    27421 cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt gatggcggcg
    27481 atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct ggacgaaggt
    27541 catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat caccgtgccc
    27601 tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat ggagcagttt
    27661 cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca ttgtgaagag
    27721 ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc tgccgggcag
    27781 gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga gatctgccag
    27841 cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct taacgattta
    27901 agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt acagatgaac
    27961 cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc tctggcgcaa
    28021 tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg gcagctacac
    28081 ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct gctgtggcgc
    28141 agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag tttttcgcgt
    28201 ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc gctggattcc
    28261 ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt tgagccttcc
    28321 atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga acaggtggag
    28381 agcaaaaaat atctcggtat gttggtactg tttgccagcg gacgggcgat gcagcgcttt
    28441 ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca gccgcgttat
    28501 cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt gctggtgggc
    28561 ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca ggtgcatatc
    28621 cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg ggaatggctg
    28681 aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc gtttaacctg
    28741 attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt ggttatctac
    28801 gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt accggtattt
    28861 ccgatagagc aaccggaagt ccctgaaggt atagttaaaa agaaagaaaa aacgaaatcc
    28921 ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat ggactatcgc
    28981 aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga ccgggatact
    29041 gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga gttgggcggc
    29101 gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg cttttacgaa
    29161 gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat gccgattgtc
    29221 atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt ggcgattctg
    29281 ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc aactcgcgtg
    29341 ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg cgggcaggcg
    29401 caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt ttgcccgccg
    29461 ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc acataccctg
    29521 gcgaaactgg cgacgccatt tgccgaaggt gaggcactgt gtctttccag cgtttcgcat
    29581 ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg ggcgttacta
    29641 atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca gatcaatctc
    29701 attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg tagcgagtta
    29761 ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg cctggctggc
    29821 agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt ggctacgggt
    29881 gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcatttta atttttatct
    29941 cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat tcctgcatct
    30001 ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca taacataaac
    30061 aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac agttttattc
    30121 aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt gccgatcatt
    30181 taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc ccaagctatg
    30241 tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc gttaaagagg
    30301 cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg gcacacgaag
    30361 cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg gcgctacata
    30421 actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg gcggggtttg
    30481 tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc cacggtcgcg
    30541 acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat aatttcccgc
    30601 tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc gcctggcata
    30661 aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg acgaccaatc
    30721 cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa cataaaggct
    30781 acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt aaaacgacgc
    30841 atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc actatcatca
    30901 tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc tttgccgagt
    30961 gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc gagtgggaag
    31021 tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga agctggcagg
    31081 ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag gctttctgtc
    31141 agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca agggtaacat
    31201 aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga aatacttgtg
    31261 ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg cctgataaga
    31321 cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct tatccgtcct
    31381 acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt ggcggaagtg
    31441 atgctgtatc cactggcacc ggctgccgcc gctttctcag ccagtttcgc ttccagggca
    31501 tcgagggtgg atgcgccatc ggcagaaacc acgccgattt tattcatgtt ctgtgcctgg
    31561 gatgccgtta ccggttccgc cgcgaacacg ccaaatgaca gagttgaaag agccatagca
    31621 gcaacaacag tattgatagt tttcataatt aatctctcgc aggtgatttt tgtataagtg
    31681 acgttgtttc gtcgatgtga tgagtatcac gtttttttac gagagataaa atcgaagaga
    31741 attgaccgcc ttgttcaaat aaattgattg atatctaaca tactgataaa ccatttattt
    31801 gttgtactta tggacttgtt ttatgccgcg tggcagtcac agcagcgtta aaggcacgga
    31861 atgccacgca aaatacaaat tatactcgct atgcatctcg ccagttgtag ttaacgtaac
    31921 tgtctgtctt tcgaacctct gcgattatat cctgtatacg ctggtttttg taaatctttc
    31981 tcctgctgac aatgaataca taagcgcacg ccaggaatgg cttcccgacg ggcttgcggg
    32041 atgggggcac cgcactcttc acattcatac aggctttcgc cacgcggaat ttcaccccga
    32101 gcgcgggcaa tcgcatcttc aattgtactg ttgatctgtt cgttgacggc gtcatcgtta
    32161 gcccaagcgg atgccatatc gtcctcccca tatcaatgct tgtacagtta agtgtagcta
    32221 atatagggac gaacctgggc agttcaagcg tcagatctcc gaccattccc gcagcagatt
    32281 atgataaaga ttgagcagcg acaggatctc ttcattttca ccatagcggc ttttcagcga
    32341 ctgaatattt ttgtccagtt caaacagcat ggcgcgcttt ttatcatcgc ggatcatcga
    32401 ctggatccac ataaatgatg ccactcgtac gccgcgggtt acgggtgtca cgcaatgcag
    32461 gctgctggag ggatacaaca ccagatcacc tgccgggagt tttacccgat gttgtccaaa
    32521 ggtgtcatta acgaccagtt cgccgccgtc gtagctttgt ggatcgctta aaaacagcgt
    32581 ggcagaaagg tcagtgcgca tccagccgtt ttgtggatgg ctgcgtactg cgccatccac
    32641 atggaaacca taggtttcat tgttctgata gcgattaaac agcggcgtgg aaagggtacg
    32701 cggcaaggca gcggcaaaga acaatgcatg ttggttaacc gcgttcagca cctcattttg
    32761 cagtgcggcg tataacgcgc tgcgggtgtc tacttgctga ttgttcttaa cttgtgcacc
    32821 ttgtgctccg gtggtgacgc gtccatccac ccattcggct tgttccagtt gttcgcgaaa
    32881 atgggcgacg tcctgtggcg ataacacgcc gggaatgtgg tacatcatac aagttctcca
    32941 aaaagtgggg cctgcgcccc acatctgaat cagaaatgca tattggctgt gagcaagaag
    33001 gttcttggct cgcccgggtg ataacggtag ccgctcttgt tgattgaggc gacgtaatcg
    33061 gtatcaaaca ggttgtaaac gtttagctgg aagtcgagat tgcgattaac ccgatacccc
    33121 agtttggcat cggcgaccca gtaaccttct gtaaatgctg gcgttcccac cgcgccgtct
    33181 gaacctttat gtatactgcc gatatagcgt gcgcccgcgc caacagagat atcatcggtt
    33241 gcctgatatt ggctccataa ggtgaaggcg tgctccgggg tatacggcag cgatgaggaa
    33301 ccatcctggg caacatcttt gccgtttttg atggttgctt tttgctgggt atagccgcca
    33361 atcacctgcc acgcgggagt gatattccct gccacggata tctcatagcc ttcgacgcgt
    33421 ttcttaccgt attgcgagta agtcccgtca tcattttgct caacttcatt ttcgatatca
    33481 gtacggaaca gcgcggcggt gagcaacagg cgtttatcca gaacctgcca tttggtgcca
    33541 atctcgctgg tgttggcttt ttgcggttta aaatcggtgc ggttggcact gttaccactg
    33601 ccagactgcg caagggcgaa gttgttgccg cccggaggct gctgggaaac ggcatagtta
    33661 atatagacat tgccgttttc cgtcaggtgg tacagcgccc cggctttcca gttcaccaga
    33721 ttgcccgact tggcggtgtc gacggtggtg actggagaac cttttgccac accagctggg
    33781 caggtgatgg caccgcgtcc gctgccgccg caggcggtgg cactgtcata ttcagtatga
    33841 taattatcca gacggatccc gccgttcagc tcaaaatcac gggtgatttg cagcgtatcg
    33901 aaggcgtaaa ttgcgaaggt atccgtctga ccattggcgt ttgcgccgtt gcgcgtcagg
    33961 ccgccgggat gaattctgct gtcaggatga taaatattta ccgccggtaa cgtcaccgga
    34021 ttaacgccat agttagtctg cgtttcacgg gtaaattcca cgccggtact gacatcatga
    34081 ccgatagaac cggtatagaa cgtcgaggtc aggttggtct ggttggtgag aattttatta
    34141 ctcacatctt tggtattcgc cgtgcgtgac taggtccagc tattcacatc gctggtgggc
    34201 tgagtaatat tcgacgcccc gcccataatc gccgtcatca ggtaatcctg ctttacgcgc
    34261 gaccaacggg tagtattgcg aatggtggtg ttatcgttga tgtcgtgctc aaaacgcatg
    34321 gtggcggtgt cggtggtcga atcgtcgtaa tcggaatccg tgccgtaaaa gttatgagta
    34381 tcaacttttc cggaatgatt cagggccgcc gctcccgcag atggggcaga atagcccggc
    34441 aaaccgatgg tcggaatgcc gccgtctggc gtgttgtgct gagtgacatg cagataatta
    34501 agatacaaac gatttgctgt accaaggcca aaagcgacag aaggggcgac gccgtaacgc
    34561 tcatttttga ctttgtcgcg tccggcatcg tgggtttttt cgcccatcac attcaggcgc
    34621 accgcggtag tatcaccaat gacctgattg acgtccagcg tgccgcggcg gaaccaggcg
    34681 ctgccaatac tggcggaggc gtcaatgtcg gaatcattgc gcggctgctt gctgatcata
    34741 ttgatcgagc ctgtcggtgc gctgcgcccg tagtcggtgc cggacggccc tttaatcact
    34801 tcgacctgct cggtattgaa ggtgtcgcgc gagacgctgc cgatatcgcg aataccatca
    34861 atataaatac tgttagaggt atcggcacca cgcatataaa tggcgtcgcc agtggtggag
    34921 ttaccgttct cacccgcaaa aaacgcaccc acgccgg
//
LOCUS       DAJTYH010000034        34712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTYH010000034 DAJTYH010000000
VERSION     DAJTYH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            677..2962
                     /gene="nrdA"
                     /locus_tag="OO609_001632"
     CDS             677..2962
                     /gene="nrdA"
                     /locus_tag="OO609_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6633091.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3196..4326
                     /gene="nrdB"
                     /locus_tag="OO609_001633"
     CDS             3196..4326
                     /gene="nrdB"
                     /locus_tag="OO609_001633"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6633092.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4326..4580
                     /gene="yfaE"
                     /locus_tag="OO609_001634"
     CDS             4326..4580
                     /gene="yfaE"
                     /locus_tag="OO609_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6633093.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4634..5284)
                     /gene="inaA"
                     /locus_tag="OO609_001635"
     CDS             complement(4634..5284)
                     /gene="inaA"
                     /locus_tag="OO609_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6633094.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5463..5705
                     /locus_tag="OO609_001636"
                     /pseudo
     CDS             5463..5705
                     /locus_tag="OO609_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5747..6823)
                     /gene="glpQ"
                     /locus_tag="OO609_001637"
     CDS             complement(5747..6823)
                     /gene="glpQ"
                     /locus_tag="OO609_001637"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6633095.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6828..8186)
                     /gene="glpT"
                     /locus_tag="OO609_001638"
     CDS             complement(6828..8186)
                     /gene="glpT"
                     /locus_tag="OO609_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6633096.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8459..10087
                     /gene="glpA"
                     /locus_tag="OO609_001639"
     CDS             8459..10087
                     /gene="glpA"
                     /locus_tag="OO609_001639"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6633097.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10077..11336
                     /gene="glpB"
                     /locus_tag="OO609_001640"
     CDS             10077..11336
                     /gene="glpB"
                     /locus_tag="OO609_001640"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6633098.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11333..12523
                     /gene="glpC"
                     /locus_tag="OO609_001641"
     CDS             11333..12523
                     /gene="glpC"
                     /locus_tag="OO609_001641"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6633099.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12716..13666
                     /locus_tag="OO609_001642"
     CDS             12716..13666
                     /locus_tag="OO609_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6633100.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13707..14177)
                     /locus_tag="OO609_001643"
     CDS             complement(13707..14177)
                     /locus_tag="OO609_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6633101.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14232..15434)
                     /locus_tag="OO609_001644"
     CDS             complement(14232..15434)
                     /locus_tag="OO609_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6633102.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15521..16063)
                     /locus_tag="OO609_001645"
     CDS             complement(15521..16063)
                     /locus_tag="OO609_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633103.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16342..16767
                     /gene="nudI"
                     /locus_tag="OO609_001646"
     CDS             16342..16767
                     /gene="nudI"
                     /locus_tag="OO609_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6633104.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16806..17408)
                     /locus_tag="OO609_001647"
     CDS             complement(16806..17408)
                     /locus_tag="OO609_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6633105.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17716..18855
                     /gene="arnB"
                     /locus_tag="OO609_001648"
     CDS             17716..18855
                     /gene="arnB"
                     /locus_tag="OO609_001648"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6633106.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18859..19827
                     /gene="arnC"
                     /locus_tag="OO609_001649"
     CDS             18859..19827
                     /gene="arnC"
                     /locus_tag="OO609_001649"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6633107.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19827..21808
                     /gene="arnA"
                     /locus_tag="OO609_001650"
                     /pseudo
     CDS             19827..21808
                     /gene="arnA"
                     /locus_tag="OO609_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21805..22695
                     /locus_tag="OO609_001651"
     CDS             21805..22695
                     /locus_tag="OO609_001651"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6633108.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22695..24347
                     /gene="arnT"
                     /locus_tag="OO609_001652"
     CDS             22695..24347
                     /gene="arnT"
                     /locus_tag="OO609_001652"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6633109.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24344..24679
                     /gene="arnE"
                     /locus_tag="OO609_001653"
     CDS             24344..24679
                     /gene="arnE"
                     /locus_tag="OO609_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6633110.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24679..25065
                     /gene="arnF"
                     /locus_tag="OO609_001654"
     CDS             24679..25065
                     /gene="arnF"
                     /locus_tag="OO609_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6633111.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25059..25331)
                     /gene="pmrD"
                     /locus_tag="OO609_001655"
     CDS             complement(25059..25331)
                     /gene="pmrD"
                     /locus_tag="OO609_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6633112.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25436..26791)
                     /gene="menE"
                     /locus_tag="OO609_001656"
     CDS             complement(25436..26791)
                     /gene="menE"
                     /locus_tag="OO609_001656"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6633113.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26788..27750)
                     /gene="menC"
                     /locus_tag="OO609_001657"
     CDS             complement(26788..27750)
                     /gene="menC"
                     /locus_tag="OO609_001657"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6633114.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27750..28607)
                     /gene="menB"
                     /locus_tag="OO609_001658"
     CDS             complement(27750..28607)
                     /gene="menB"
                     /locus_tag="OO609_001658"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6633115.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28622..29380)
                     /gene="menH"
                     /locus_tag="OO609_001659"
     CDS             complement(28622..29380)
                     /gene="menH"
                     /locus_tag="OO609_001659"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6633116.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29377..31047)
                     /gene="menD"
                     /locus_tag="OO609_001660"
     CDS             complement(29377..31047)
                     /gene="menD"
                     /locus_tag="OO609_001660"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6633117.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31136..32431)
                     /gene="menF"
                     /locus_tag="OO609_001661"
     CDS             complement(31136..32431)
                     /gene="menF"
                     /locus_tag="OO609_001661"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6633118.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32510..32815)
                     /gene="elaB"
                     /locus_tag="OO609_001662"
     CDS             complement(32510..32815)
                     /gene="elaB"
                     /locus_tag="OO609_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6633119.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32870..33331)
                     /locus_tag="OO609_001663"
     CDS             complement(32870..33331)
                     /locus_tag="OO609_001663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6633120.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33396..34313
                     /gene="rbn"
                     /locus_tag="OO609_001664"
     CDS             33396..34313
                     /gene="rbn"
                     /locus_tag="OO609_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6633121.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34504..34692
                     /locus_tag="OO609_001665"
                     /pseudo
     CDS             34504..34692
                     /locus_tag="OO609_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8482 a         9299 c         8604 g         8327 t
ORIGIN      
        1 tatataagac ggcatattta aacgcattat acaatatggg ttatctttct tttgtaaatt
       61 gtccaacccg atgcaatggc tattttgtta ttatttgatg aataatatca gtgcgtcata
      121 attcaagtta ataaccttaa gggataccag ttatatttaa actaaattaa agtaatgaat
      181 aattttctta taatataagg taaattaaca aaatggcttc gcatttaaca ataaccgaat
      241 agaaaacaac catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact
      301 gatacatcag attatgtgat gactcgtgct tagatcaatt tttgcaatca ttagcaaaaa
      361 gattaataaa ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga
      421 acagttattt ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc
      481 taaggtgcgc gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa
      541 gagggtcatt ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt
      601 ctaagcagct tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa
      661 aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga
      721 gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa
      781 cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac
      841 ctctgacatc cacgaaacta ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc
      901 ggattatcag tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg
      961 ccagtttgag ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata
     1021 cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat
     1081 cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata
     1141 tctagtacag aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc tttatattct
     1201 tgttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat acgtgaagcg
     1261 tttttatgac gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt
     1321 gcgtaccccg actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga
     1381 ttccatcaac gccacctcca gcgcgattgt gaaatatgtt tcccagcgtg ccgggatcgg
     1441 catcaacgcc ggacgtattc gtgcgctggg cagcccgatt cgcggtggcg aagcgttcca
     1501 taccggctgt atcccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg
     1561 cggtgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag
     1621 cctgctggtg ttgaaaaaca accgtggggt tgaaggcaac cgcgtgcgtc atatggacta
     1681 cggggtacaa atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag atatcaccct
     1741 gttcagcccg tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt
     1801 tgaacgcctg tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc
     1861 cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca
     1921 gaacgttgac cactgtaata cccatagtcc gtttgatccg accatcgcgc cagttcgcca
     1981 gtctaacctg tgcctagaaa ttgccctgcc gaccaaaccg ctgaacgacg tcaacgacga
     2041 gaacggtgaa atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaatagcct
     2101 ggatgaactg gaagagctgg cgattctggc ggttcgtgca cttgacgcgc tactggatta
     2161 tcaggattac ccgatcccgg ccgccaaacg tggagcgatg gggcgtcgta cgctgggtat
     2221 tggtgtgatt aacttcgctt actatctggc gaagcacggt aaacgctact ccgacggcag
     2281 cgccaacaac ctgacgcata aaaccttcga agcgattcag tattacctgc tgaaagcgtc
     2341 taatgagctg gctaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa
     2401 agggatcctg ccgatcgata cctataagaa agatctggat gccatcgcta atgagccgct
     2461 gcattacgac tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactccac
     2521 gctttctgcc ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat
     2581 tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt
     2641 ggtgccggac tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa
     2701 cgatggctac ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc
     2761 taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct
     2821 gaaagacctg ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg
     2881 tgacggcgct gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga
     2941 aagcggcgca tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg
     3001 cctacggctc ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc
     3061 cggatgcagc gtgaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg
     3121 cgccagcgtc gcatcaggca caggatgcgg cgtaaaatgc cttatccggc attaaactcc
     3181 caacaggaca cactcatggc atataccacc ttttcacaga cgaaaaatga tcagctcaaa
     3241 gaaccgatgt tctttggtca gccggtcaac gtggctcgct acgatcagca aaaatatgac
     3301 atcttcgaaa agctgatcga aaagcagctc tctttcttct ggcgtccgga agaagttgac
     3361 gtctcccgcg accgtataga ttaccaggcg ctgccggagc acgaaaaaca catctttatc
     3421 agcaacctga aatatcagac gctgctggat tccattcagg gtcgtagccc gaacgtggcg
     3481 ctattgccgc ttatttctat tccggaactg gaaacctggg tcgaaacctg ggcgttctca
     3541 gaaacgattc attcccgttc ctatactcat atcattcgta atatcgttaa cgatccgtct
     3601 gttgtgtttg acgatatcgt caccaacgag cagatccaga aacgtgcgga agggatctcc
     3661 agctattacg atgaactgat cgaaatgacc agctactggc accttctggg cgaaggcacc
     3721 cacaccgtta acggtaaaac cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat
     3781 ctctgcctga tgagcgttaa cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt
     3841 tcctttgcct ttgcagaacg cgaactgatg gaaggtaacg ccaagattat tcgcctgatt
     3901 gcccgcgatg aagccctgca cctgaccggt actcagcata tgctgaatct gctgcgcagc
     3961 ggcgcggatg atccggagat ggcggaaatt gccgaagaat gtaagcagga gtgctatgac
     4021 ctgtttgtcc aggcggcaca gcaagagaaa gactgggcgg attatctgtt ccgtgacggt
     4081 tcgatgattg gtctgaataa agatattctc tgccagtacg ttgaatacat caccaatatc
     4141 cgcatgcagg cagtcggttt ggatctgccg ttccagacgc gctccaaccc gatcccgtgg
     4201 atcaacacct ggctggtgtc tgataacgtg caggttgctc cgcaggaagt ggaagtcagt
     4261 tcttatctgg tcgggcagat tgacgcggaa gtggacaccg acgatttgag taacttccag
     4321 ctctgatggc ccgcgttacc ctgcgcatca ctggcacaca actgctgtgc caggatgaac
     4381 acccttccct tctggcggcg ctggaatccc acaatgtggc ggttgagtac cagtgccgcg
     4441 aaggttactg cggctcctgt cgcacccgac tggtcgcagg tcaggtggac tggattgccg
     4501 aaccgttagc ctttattcag ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg
     4561 atattgaaat cgagttgtga tcgggtacta gcgccagata cgacgcttac gcgtattatc
     4621 tggcctacaa cgattacatg gcgtagtaat acgctttcac ctgctcccag tccgctttag
     4681 ggatcggctc cagatatttt tccagctggc ggaagtcatg attaatcgct ttatcgcgac
     4741 gcaagcggcg acgacttttc tccagatcaa gaaaaccagc ttctgcctta ccttctgttt
     4801 tcacatagat atggcgaaca tagcaacagc catgctgacg attaacgcta tgcattttct
     4861 taaacgccag tgccactgct ttcaacatgg cttgccgtac ttcgtcagaa taaggcgata
     4921 ctgcatgccg ggcataccag tcagcaatgc tgatgaaccc cgccatatct tcagtcacca
     4981 gcaacgcgcg ccattcaccc tcaattttca ccgcttcacc aaaaacgatc ttcggcacga
     5041 tgacaccggc ccgttccagt tctttaatta ccgcaacctc acggacaatc gttggtcggc
     5101 cgaacggata acgtactgaa tgaaacagat gatgcgtcat gcgctttaca tacagctttt
     5161 tgccgttgcg ctcgacgcat tgcaccccgc tcataccatt acggcgatag ttaggctctt
     5221 caacccagtc gccctctgtt gcccaccagt gattaaattc gtcgtacttt gctgaaactg
     5281 ccatacccta tcgcctgtca tttttattaa cgacaatgac tataggtggt tacctgagga
     5341 aaatcttaat gaaacgtgtc gtattaatga atttcttttg ccgctacagg tgtgacaccg
     5401 cttttgccct tcatttgcag cgcatagatt ccaccagcca ctaccactaa tagtgcgccc
     5461 agcagataaa atgtgaattt aatcagtcgg atacagcgat gaattttatt cgacagggat
     5521 taggcatcgc cttacaacca gagttaacgc tgaaaagcat tgcaggtgaa ttgtgttccg
     5581 ttcctcacga accaaccttc tatcgacaga tttcgttgct gactaaagaa aagccggtag
     5641 aaggcagtcc actgttttta ctacaaatgt gcatggaaca attagtggcg attggaaaaa
     5701 tttgatatga gacaggatgg cgcatgaacg ccatcctgca gtaacattac tctttattaa
     5761 gaaattttac tgccttatca gggaaatcag taaacagccc atttacaccc gctttgttat
     5821 acagagcatc atataactga ttcacatcag gagtgtattc aggcagttta tctgaccgca
     5881 cggtataagg atgcactacc agtttattct gctgggcatc ttgcaccatg ccagtgagtt
     5941 tgatattacc cggctgcgat gtctcctcaa tcaacatatg gtaatccgga ccaataccat
     6001 ctgcatattc cgccacctgt ttcatggctc ccggcttaaa catccagtcg tagttgtaat
     6061 taacccagct tccatccggc tgtttctgct gcgtttcatt ccagtcggta taggcaatca
     6121 gctgtaccag attgagctcc atgcccattt tgggttccag ctcattctta atacgcttca
     6181 gctcatcagc atcaaaacat tgcaaataaa ctttatcgtc tttaccggtg taaccatatt
     6241 tcttcagcac ttccagcgtt tttgccgcaa tatccttccc ttcctgatga tggaaccacg
     6301 gcgctttgat ttctggatag ataccgatat ttttcccggt agagtgattt aacccctgaa
     6361 caaattcaat ctcttcttca aaggtgtgca cccggaagtc ggacttaccc attgggaaac
     6421 gccccggata agtctgtact tttttaccgt tttcaatatc gaaaccttcg gtaaatttca
     6481 gcgacttaat ttcatccagc gtgaaatcta tcgcgtagta acgaccgtct ttgcgcgccc
     6541 gatccgggaa acgatcggca acatcagtaa cacgatcgag ataatggtca tgcagaacaa
     6601 ccagatggtc gtctttggtc atcaccaaat cctgttccag ataatccgct ccctgcgcat
     6661 acgccatcgc ttttgctggc agcgtatgct ccggcaaata tccactggca ccgcgatggg
     6721 cgatgactat tttttcgttg ctgtccgccg ccattgcact gcttcccatg actatagtgc
     6781 tcatcatgat cgccatgcta aggtttttca gcttcaattt catgccatta gcctccgttg
     6841 cgtttttgca gtaattgttc atggcgacgt ttttcgccaa tcatcacaac aatcaacaag
     6901 ataaccgcca gaatgctgcc gccaatcatt accataaagc cgccatccca gccgaagaag
     6961 tccacggtgt agccaacaat cgcgctcgcc gccaccgaac cgcccaggta accaaacagc
     7021 ccggtaaagc ccgctgccgt acctgccgct tttttcggtg ccagttccag cgcatgcaga
     7081 ccgatcagca tcacaggacc gtagatcagg aagccgataa caatcataca aatcatatcg
     7141 acggttgggt tacctgccgg gttcatccag taaacgatag tcgcgatggt caccagtgtc
     7201 ataaagaaga cgccggttgc cccacggtta ccacggaaga ctttgtccga catccagccg
     7261 cacagcaggg tgcccggaat acccgcatac tcatagaaga agtaggccca ggaggattta
     7321 tccagcgcga aatgcttaac ctctttcaga taagtcggtg accagtcgag gatgccgtaa
     7381 cgcagcagat aaacgaacac gttggcgatg gcgatgtacc acagcagttt gttcggcagc
     7441 acgtactgca tgaagatttg cttcgcggtc agctcctgtt ccgctttttc gttatagtcg
     7501 tccggataat catttttgta ctcttcgatc ggcggcaagc cacaggattg cggggtatcg
     7561 cgcatcatcg caaaggcgaa taatgccacc agaatggcgc agaaagcagg catatagagc
     7621 gccgcatgcc agtcattgaa ccaggccatc cccagcagga acagcagcgg cggaatacca
     7681 ccaccgacgt tgtgcgcaca gttccacact gacacaatgc cgccacgttc tttctgcgac
     7741 caccagtgca ccatagtacg accacacggc ggccacccca tcccctggaa ccaaccgcag
     7801 aggaacaaca gtacaaacat caccgcaatg ctcgacgtcg cccatggcac aaagcccata
     7861 aacaacatca ctgccgccgc cagaatcaaa cctgcgggca ggaaaacgcg cggattcgag
     7921 cgatccgata ccgaacccat gatgaatttc gaaaatccat aagcaatcga gatccccgaa
     7981 agggcaaaac ctaaatcacc gcgtgagaat ccctgctcaa ccagataagg catagcaagc
     8041 gcaaagttct tacgaaccaa atagtaagcc gcatagccaa agaatatccc caggaaaatt
     8101 tgccagcgca atcgacgata agtcggatcg atctccgcgg caggtaagcg cgctttgtgt
     8161 ggcgctggtt taaaaatact caacattgat agcctccgtg gcccgtggtc ttatttatga
     8221 ttaacagcct gattcagtga gagaacctgc cgtttcttga gttgccgcga tgttaagaaa
     8281 acattcataa attaaatgtg aattgccgca cacattatta aataagattt acaaaatgtt
     8341 caaaatgacg catgaaatca cgttttactt tcgaatcatg agcgaatatg cgcgaaatca
     8401 aacaattcat gtttttacta tggctaaatg gtaaaaaacg aacttcagag ggataacaat
     8461 gaaaactcgc gactcgcaat caagtgacgt gattatcatt ggcggcggcg caacgggagc
     8521 cgggattgcc cgcgactgtg ccctgcgcgg gctgcgcgtg attttggttg agcgccacga
     8581 catcgcaacc ggtgccaccg ggcgtaacca cggcctgctg cacagcggtg cgcgctatgc
     8641 agtaaccgat gcggaatcgg cccgcgaatg cattagtgaa aaccagatcc tgaaacgcat
     8701 tgcacgtcac tgcgttgaac caaccaacgg cctgtttatc accctgccgg aagatgacct
     8761 ctcattccag gccactttta ttcgcgcctg cgaagaagca gggatcagcg cagaagctat
     8821 agacccgcag caagcgcgca ttatcgaacc tgccgttaac ccggcactga ttggcgcggt
     8881 gaaagttccg gatggcaccg ttgatccatt tcgtctgacc gcagcaaaca tgctggatgc
     8941 caaagaacac ggtgccgtta tccttaccgc tcatgaagtc acggggctga ttcgtgaagg
     9001 cgcgacggtg tgcggtgttc gtgtacgtaa ccatctcacc ggcgaaactc aggcccttca
     9061 tgcacctgtc gtggttaatg ccgctgggat ctgggggcaa cacattgccg aatatgccga
     9121 tctgcgcatt cgcatgttcc cggcgaaagg atcgctgctg atcatggatc accgcattaa
     9181 ccagcatgtg atcaaccgct gccgtaaacc ttccgacgcc gatattctgg tgcctggcga
     9241 taccatttcg ctgattggta ccacctcttt acgtattgat tacaacgaga ttgacgataa
     9301 tcgagtgacg gcagaagagg ttgatattct gctgcgtgaa ggggaaaaac tggcccccgt
     9361 gatggcgaaa acgcgcattt tgcgggccta ttctggcgtg cgcccgctgg ttgccagcga
     9421 tgacgacccg agcggacgta acgtcagccg tggcatcgtg ctgctcgacc atgctgaacg
     9481 cgatggtctg gacggattta tcaccatcac cggtggcaaa ctgatgacct atcggctgat
     9541 ggctgaatgg gctaccgacg cggtatgccg caaactgggc aacacgcgcc cctgtacgac
     9601 tgccgatctg gcactgcctg gttcacaaga acccgctgaa gttaccttgc gtaaagtcat
     9661 ctccctgcct gccccgctgc gcggttctgc ggtttatcgt catggcgatc gcacgcctgc
     9721 ctggctgagc gaaggccgtc tacaccgtag cctggtatgt gagtgcgaag cggtaactgc
     9781 gggtgaagtg cagtacgcgg tagaaaattt aaacgttaat agcctgctgg atttacgccg
     9841 tcgtacccgt gtggggatgg gcacctgcca gggcgaactc tgcgcctgcc gcgctgccgg
     9901 actgctgcaa cggtttaacg tcacgacgtc cgcgcaatct atcgagcaac tttccacctt
     9961 ccttaacgaa cgctggaaag gcgtgcaacc catcgcctgg ggagacgcac tgcgcgaaag
    10021 cgaatttacc cgctgggttt atcagggact gtgtggtctg gagaaggagc agaaagatgc
    10081 gctttgatac tgtcattatg ggcggcggcc tcgccggatt actctgtggc ctgcaactgc
    10141 aaaaacacgg cctgcgctgt gccattgtca cgcgtggtca aagcgcgttg catttctcat
    10201 ccggttcgct ggatttgctg agccatctgc cagatggtca acccgtggca gacattcaca
    10261 gtggactgga atctttgcgt cagcaggcac ctgcacatcc ttactctctt ctcgggccac
    10321 aacgcgtgct cgatctcgct tgccaggcgc aggcattaat cgctgaaagc ggtgcgcaat
    10381 tgcagggcag cgtagaactg gctcaccagc ggattacgcc gctcggcact ttgcgtgcaa
    10441 cctggttaag ttcgccagaa gttcccgtct ggccgctacc cgcgaagaag atatgtgtag
    10501 tggggataag cgggctgatg gatttccagg cgcatcttgc ggcggcttcg ttgcgcgaac
    10561 tggatctttc ggttgaaacc gcagaaatag agctgccgga actggatgtc ctgcgcaata
    10621 acgccaccga atttcgcgcg gtgaatatcg cccgtttcct tgataatgaa gaaaactggc
    10681 cgctgttact tgatgcgctt attcctgtcg ccaatacctg cgaaatgatc ctgatgcccg
    10741 cctgcttcgg cctggccgat gacaaactgt ggcgttggtt gaatgaaaaa ctaccttgtt
    10801 cactgatgct tttgccaacg ctgccgcctt ccgtgctggg cattcgtctg caaaaccagt
    10861 tacagcgcca gtttgtgcgc caaggtggag tgtggatgcc gggcgatgaa gtgaaaaaag
    10921 tgacctgtaa aaatggcgta gtgaatgaaa tctggacccg caatcacgcc gatattccgc
    10981 tacgtccacg tttcgcggtt ctcgccagcg gcagtttctt tagtggcgga ctagtagcgg
    11041 aacgtaacgg cattcgcgag ccgattctcg gccttgatgt gctacaaacc gccacgcggg
    11101 gtgaatggta taagggcgat ttttttgcgc cgcaaccgtg gcagcagttc ggtgtaacca
    11161 ctgatgagac gctacgcccg tcgcaggcag ggcaaaccat tgagaacctg tttgccatcg
    11221 gttcggtgct gggcggattt gatcccatcg cccagggatg cggcggcggt gtttgtgccg
    11281 tcagtgcttt acatgccgct caacagattg cccaacgcgc aggaggccaa caatgaacga
    11341 caccagcttc gaaaactgca ttaagtgcac cgtctgcacc accgcctgcc cggtgagccg
    11401 ggtgaatccc ggttatccag ggccaaaaca agccgggccg gatggcgagc gtctgcgttt
    11461 gaaagatggc gcactgtatg acgaggcgct gaaatattgc atcaactgca aacgttgtga
    11521 agtcgcctgc ccgtccgatg tgaagattgg cgatattatc cagcgcgcgc gggcgaaata
    11581 tgacaccacg cgcccgtcgc tgcgtaattt tgtgttgagt cataccgacc tgatgggtag
    11641 cgtttccacg ccgttcgcac caatcgtcaa caccgctacc tcgctgaaac cggtgcggca
    11701 gctgcttgat gcggcgttaa aaatcgatca tcgtcgcacg ctaccgaaat actccttcgg
    11761 cacgttccgt cgctggtatc gcagcatagc ggctcagcaa gcacaatata aagaccaggt
    11821 cgctttcttt cacggctgct tcgttaacta caaccatccg cagttaggta aagatttaat
    11881 taaagtgctc aacgcaatgg gtaccggtgt acaactgctc agcaaagaaa aatgctgcgg
    11941 cgtaccgcta atcgccaacg gctttaccga taaagcacgc aaacaggcaa ttacgaatgt
    12001 agagtcgatc cgcgaagctg tgggagtaaa aggcattccg gtgattgcca cctcctcaac
    12061 ctgtacattt gccctgcgcg acgaataccc ggaagtgctg aatgtcgaca acaaaggctt
    12121 gcgcgatcat atcgaactgg caacccgctg gctgtggcgc aagctggacg aaggcaaaac
    12181 gttaccgctg aaaccgctgc cgctgaaagt ggtttatcac actccgtgcc atatggaaaa
    12241 aatgggctgg acgctctaca ccctggagct gttgcgtaac atcccggggc ttgagttaac
    12301 ggtgctggat tcccagtgct gcggtattgc gggtacttac ggtttcaaaa aagagaacta
    12361 ccccacctca caagccatcg gcgcaccact gttccgccag atagaagaaa gcggcgcaga
    12421 tctggtggtc accgactgcg aaacctgtaa atggcagata gagatgtcca caagtcttcg
    12481 ctgcgaacat ccgattacgc tactggccca ggcgctggct taaactccct tctgatgccc
    12541 ggtaagcatg tggttaccgg gcatttttgc gtacacgatt ccgtgcccaa tgtatgcgtt
    12601 gcaacgcagt gaaaattcct ctgaaaacgt ctcgcaaagg ctgaaactgg cagatgtcaa
    12661 aggcctggga taaccgtaat gtcgcgtcat cataaatatc aggtgacgga caaccatgac
    12721 cgaatcaaca acctcctccc cgcatgatgc ggtatttaaa acctttatat tcacacccga
    12781 aaccgcacgg gattttctcg aaatacattt accagaacca ctgcgcaagc tttgcaacct
    12841 gcaaacctta cgcctggaac ccactagttt tattgaaaaa agtttacgcg cttactactc
    12901 ggatgttttg tggtccgtgg aaaccagcga cggtgacggc tatatctact gcgtgattga
    12961 acatcaaagc tctgcagaaa agaatatggc ttttcggcca atgcgctatg ccactgccgc
    13021 catgcagagt cacctagata aaggctatga cagagttccg ctggtggtgc cattgctgtt
    13081 ttatcatggc gaaacatcgc cctacccgta ctcacttaac tggctggatg agtttgacga
    13141 tccgcaactt gcccggcagt tgtacaccga agcttttccg ttggtggata tcaccatcgt
    13201 acctgacgat gagatcatgc aacatcggcg tatagctctg ctggaactga ttcaaaagca
    13261 tattcgcgac cacgatttaa tcggcatggt cgacaggatc accacgcttt tggttagagg
    13321 cttcactaat gacagccagc tacaaacact gtttaattat ctgctgcaat gcggcgatac
    13381 ctcccgtttc acccgtttta ttcaggagat tgccgaacgt tcaccactac aaaaggagag
    13441 attaatgact attgctgaac ggctacggca ggaaggacat caaattggct ggcaagaagg
    13501 taaattagta ggtttgcagc aaggtaaatt agaaggtttg caggaaggta tgcatgaaca
    13561 agccattaaa attgctttgc gcatgctgga acagggcatt gatcgtgacc aagtgctcgc
    13621 ggccacccag ctaagcgaag ccgatctggc agcgaataac cactaattaa cacaggccca
    13681 cagccgatcc ccatgggcct ttgatatcaa taactacctt ttatgcgtgg cccatttttg
    13741 cctgatttaa acatcgccag tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg
    13801 ccaacagcga caaagttcgc tccccacgcc aggcattgct gcgccatatc aggagccaca
    13861 gccagaaaac cagccgcttt acccgcagca cggatccgcc gaatgctggt ttcaataatt
    13921 cgctgcactt ccgggtgccc ggcgttatcc gggtagccca acgacgcaga aagatccgct
    13981 ggtccaataa acacgccatc aatcccttcg acgtcgagga tctcgtccag gttatccagt
    14041 gccgttttac tttccacctg cactaacaga caaagcgaat cgttgacttg cgccatgtaa
    14101 ttctcaatgc gtccccagcg cgcagcccgt gccacactgg ccccaacacc acgctcaccg
    14161 tagggagggt tggacaaatt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca
    14221 gagtggcgaa atcagacgga taaagtcatg gactcaacaa cctcaatcca gccatgctca
    14281 ctagcgatgt cctggccgtt taaccaacgg cgtagcatat tcagtgccat cattgcgcac
    14341 acttcctgac ggatagccag gctgtagcgt gtagtgctga aacgcacacg cagagcgaaa
    14401 gtgccgtctg gcgtggctag cgcaaagttg agatgctcgt tctcgaaacc cgaaacagcc
    14461 agtgccagcc cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc
    14521 agggtttctt cctgtgaagg aatcacttca cacgccagca atggagcacc tgcgcgagaa
    14581 agttgcaaag ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg
    14641 ttttgcaatt cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc
    14701 tgtccggcaa cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc
    14761 ggtccggtga gtttcagttc gatgatcggc attgaggagc gatagcccat tgttacgccc
    14821 ggcggcagtt gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta
    14881 gttaaacgca gacaaaccgg cggctgtggt aaagaaaaac gctcgcgcag gcgcggcagg
    14941 atttcgtgct cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg
    15001 caacgattaa gctgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca
    15061 gcactggcag gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg
    15121 tggaaatagc gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt
    15181 gctgtcgcag cggcgagtgc gcttaaatca tcgctggtcg gtcccagccc gccgttaacg
    15241 atcagcacat cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta
    15301 tcccccaccg tattgcggcg agataatggc agcccctgat gaaagaaaaa atcggccagc
    15361 caggcagcgt tagtgtcaac gatttgcccg tataacactt catccccggt ggatagcatt
    15421 tccactttta acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag
    15481 ataaggcgta gtaagaaact gaatgccgcg ccggaaggga tcagaaactg gcgttaaccc
    15541 caacatacag gccgtcagcc acagcgttgt cgcggttacc gtctttaccc gacagattca
    15601 ggtagcgata acccgcctca atactgactg gacgcataat ggtgtaacgc gcgccagcat
    15661 tcgcttcctc ataactttga ataccgctgg agagcgaatc cggagagtag taatactcgc
    15721 caaacaaacg gaagctgttg ccaattttcc actgcaaacc acctcctacc gctgcggcat
    15781 aaccttcatc gccgtaattc gggttggtgt acacgccttt tccgccaacg gtcgccatta
    15841 acggcccgag aggcagattc aaccccagcc ccacgcccgc gacgtcaccg tcgtcgtcgt
    15901 tatgtgtcca gttaccgctt aaagccaggc ccgtcgattc agtcccaaaa ccgacaccaa
    15961 tattggtgta ttctttaccc gcctggccgc tgatgctcat tgcattgacc gatgcagaaa
    16021 ccaacagcat tccggccaga cctgctagcg caattttttt cattatcccc tttcccacaa
    16081 gcaattaatc aaaattccca aagctgcggg attgtactgc ctggcacaga ggattcaatg
    16141 agcgaaaaaa gggatctagt cacttattgt aactaaatac ttcttaaata attaaacgcc
    16201 gaaaaaataa acacacttta ccgatacaca caaaaataat aataagtgca gttatcattg
    16261 caaaagagat tactgtcact ttcacatatt tctgagttac cgtattctca tggtttcttt
    16321 ttatttataa ggaatacccc tgtgcgacaa cggactattg tatgcccttt gattcaaaat
    16381 gatggtgctt atttgctgtg caaaatggcc gacgatcgcg gcgttttccc cggtcaatgg
    16441 gcgctttcgg gtggcggcgt ggagtctggc gaacgaattg aagaggcact acgccgcgaa
    16501 attcgcgaag aactgggaga acagctgctt ttgacagaaa tcacgccgtg gaccttcagc
    16561 gatgatattc gcaccaagac atatgcagat ggtcgcaagg aagagattta tatgatttac
    16621 ctgatttttg actgcgtttc tgccaaccga gaagtgaaaa taaacgaaga gtttcaggac
    16681 tacgcgtggg taaaacctga agatctggtg cattatgatt taaatgttgc cacccgaaaa
    16741 acgttacgtt tgaaaggtct tctttaacaa tgatgacagc catcactggc tgtcagattg
    16801 aaaatttagt ggttaacgaa ttccccatcc agataaactt tgcctttttc cacatgcatg
    16861 actaaaccat ccagataatc aggtttaaat atcgcatcac gcttattttt agcaatatat
    16921 gtcaggcaat gattatgggt gaaaataacg atatttttat caggcgcttt gctttgtaag
    16981 tccttaattg cactataaat ctcattaccg cactgaagaa gtcgtttatc taccgtcagc
    17041 tttttacccg cggaaaacca ggtcgccgac tgaatggtcc ggacggtatt actggaataa
    17101 agatcgaaat cagagatgtc agcgctaaaa gcgttgccca gttcacgggc atcctgggta
    17161 cctttaaccg taatacctgt tttatctgac aagcattgat tggttgaacg atcgcaacgt
    17221 tcagcatggc gaaacaaaac gacaaccggg tgctgctgtg ccagtctggc caatgtttta
    17281 ttgtcgatac gtggcaaacc attgctactc caggcggcat gagtacccag tccggcaatt
    17341 gcagcgagcg ccagtaaaat gatgaaatat ttttttgact tcaatgatga gcggcaaaaa
    17401 gctaacacgt aaactccacc tatagacaag cgcaaccaga caattaccgt gaaattgagc
    17461 gacatttctg gcgataatta gcagttggtg taatattaaa aatcctacga tgtcggcaaa
    17521 atgcctcaaa tttttgccaa atgcaaagcc taaataagaa aaaatataaa aatttcaata
    17581 tttacgtcta atattagttt cttaaggtta agttaatatt ctatccttaa aatttcgctc
    17641 caaatggcaa aatatacaca acactcttta tagcaaatat aagtggacag gtattcaatg
    17701 gcggaaggaa aagcaatgtc agaatttttg cctttttcgc gaccagcaat gggcgtggag
    17761 gaactcgctg cagttaaaga ggttctcgaa tccggttgga tcacaaccgg tccgaagaat
    17821 caggcgcttg agcaagcctt ttgccagttg acgggaaatc agcatgccat cgcggtcagt
    17881 tcagccaccg ccggaatgca tatcacgcta atggcgttgg aaattggcaa gggcgatgaa
    17941 gtgattacgc cttccctgac ctgggtttca accctcaata tgatttcctt gctgggtgca
    18001 acgccggtaa tggtggatgt cgaccgcgat acgctgatgg tcacgcctga agcgatcgaa
    18061 tcagccatta cgccacgcac taaagccatc attccggtgc attatgccgg tgcgccagca
    18121 gatattgacg ccattcgcgc cattggcgaa cgttacggca tcgcagttat cgaagatgct
    18181 gcccatgccg tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg taccgctatt
    18241 ttttcatttc atgccattaa aaatattacc tgtgctgaag gtggcctgat tgtaactgat
    18301 aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc acggtctggg tgtcgatgcc
    18361 tatgacagac aaacctgggg ccgtgcaccg caggctgaag tcttaacacc gggctataag
    18421 tacaatctga ccgatattaa cgccgcgatt gccctgacac agttagtcaa attagagcac
    18481 ctcaacaccc gtcggcgcga aattgcccag caatatcagc aagcactggc agctctcccc
    18541 tttcagccat taagccttcc cgcctggccg cacgttcacg cctggcatct gtttattatt
    18601 cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc gttaaaagaa
    18661 agaggcattg gtaccgggtt acatttccgc gccgctcaca cacaaaaata ttatcgcgag
    18721 cgttttccca cgctgtcgtt accgaatacc gaatggaata gcgaacgcat ctgttcgttg
    18781 ccgctgttcc cggatatgac taccgccgat gccgaccgcg tcatcacagc ccttcagcaa
    18841 ctcgcaggac aataagccat gtttgaaatc caccctgtta agaaagtctc ggtggttatt
    18901 cccgtttata acgagcagga aagcttaccg gaattaatca ggcgcaccac cacagcctgt
    18961 gaatcgttgg ggaaagagta tgagatcctg ctgattgatg acggcagtag cgataattcc
    19021 gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc atattgtgtc tattttgatt
    19081 aaccgcaatt acgggcaaca ttcagcgatt atggcgggat tcagtcacgt tactggcgac
    19141 ttaattatta cccttgatgc cgatctccag aatccgccag aagaaatccc ccgcctggtg
    19201 gcaaaagccg atgaaggtta cgacgtggta gggactgtac gccagaaccg ccaggacagc
    19261 tggtttcgta aaacagcttc gaagatgatt aaccggctta ttcagcgcac cactggcaaa
    19321 gcgatgggtg actacggttg tatgctgcgc gcctatcgcc gtcatattgt cgatgcgatg
    19381 ttgcactgcc atgaacgcag cacctttatc ccgattctgg cgaatatctt cgcccgccgt
    19441 gcaattgaaa ttccagtaca tcatgccgag cgtgagtttg gtgaatccaa atacagtttt
    19501 atgcgcctga ttaatttgat gtacgacctg gtgacctgcc ttaccacaac gccgctacgt
    19561 atgctgagtc tgctcggcag cattattgcg attggaggtt ttagcattgc ggtgctgctg
    19621 gtgattttac gcctgacctt cggaccacaa tgggcggcag aaggcgtctt tatgctattt
    19681 gccgtgctgt ttacttttat tggcgctcag tttatcggca tgggattact cggtgaatat
    19741 atcggcagga tctacaccga tgtccgcgcc cgcccccgct attttgttca gcaagttatc
    19801 cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac cacgatatgg
    19861 gatgcctcgg tattgaagct ctgctggctg ccggttacga aattagcgcc atttttaccc
    19921 ataccgataa tcccggtgaa aaagcctttt atggttcggt ggctcatctg gcggcggaaa
    19981 gagacattcc ggtttatgcg ccggataacg ttaatcatcc gctgtgggtg gaacgcattg
    20041 cccaactgtc accagaggtg attttctctt tttattatcg ccatcttatt tgcgacgaga
    20101 tttttcagct cgctcccgca ggtgcattta atctgcacgg ttcgctgtta ccaaaatatc
    20161 gtggtcgcgc gccgctgaac tgggtgctgg tgaacggtga aacggaaacg ggcgttacat
    20221 tgcaccgaat ggtaaacgtg ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat
    20281 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
    20341 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
    20401 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa
    20461 accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
    20521 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
    20581 caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg
    20641 cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
    20701 agcgcagatg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
    20761 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
    20821 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
    20881 gataagccgt tttctgaatc atccacattt tcactttgtc gaaggcgata tcagtattca
    20941 ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
    21001 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
    21061 gaatctgcgc attatccgct actgcgttaa gtaccgtaag cgaatcatct tcccgtcaac
    21121 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
    21181 cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat tacttgatcg
    21241 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc tcccgtttaa
    21301 ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat
    21361 tacgcaactc attctcaatc tggtagaagg ttcaccaatt aagctaattg atggcggaaa
    21421 acaaaaacgc tgctttactg atatccggga cggtatcgag gcgttgtacc acattatcga
    21481 aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg agaacgaagc
    21541 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
    21601 tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agctgctact acggcaaagg
    21661 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg
    21721 ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
    21781 cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatgcc
    21841 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
    21901 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
    21961 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
    22021 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
    22081 atcattcgtg aagcggctaa acatcacgaa gtcggtctgc acgcctggga tcaccatgcc
    22141 tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt
    22201 cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg
    22261 cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
    22321 tgtcgtgggg ccataccgtt ccgtccactg cttgaatcag gaaaccctgg cactgcgcaa
    22381 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
    22441 tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
    22501 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca
    22561 gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc
    22621 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
    22681 gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg catcttcgcg tttattgcct
    22741 gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg
    22801 cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
    22861 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
    22921 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
    22981 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
    23041 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
    23101 cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
    23161 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
    23221 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
    23281 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
    23341 tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact
    23401 attttttctg ggttgagcat attcaacgct ttgcactaga tgatgcccaa catagagctc
    23461 cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
    23521 ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
    23581 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg cccacctata
    23641 ttctttcttg ctttgcacct ctggcaatgc tgttggcgca ttacgcgttg ctggcagcaa
    23701 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg
    23761 gcattattgc cacatttgtg atctccccct ggggaccaat gaacacgccg gtgtggcaaa
    23821 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
    23881 gctggtacac cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg
    23941 ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc
    24001 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
    24061 gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc
    24121 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
    24181 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg
    24241 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
    24301 tcgttcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
    24361 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
    24421 tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
    24481 tcttggtctt gccatgatgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
    24541 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
    24601 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat
    24661 cctcgggagt acggtgtaat gtgcctgata tggggattat tcagcgtcat tattgcctca
    24721 gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg
    24781 gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg
    24841 ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc
    24901 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
    24961 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
    25021 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct
    25081 gtacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta
    25141 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
    25201 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
    25261 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gtgccagcca
    25321 ttccattgca ttatcctgtt tgctaagagt tttcacatca atttacagat taacagataa
    25381 actctttatc aacggcaatg gaatggcaga aatgagaagt gaaacggagg gttaattatt
    25441 gctgacgttg cacccactct tttagcgcct gacgtgaaat tttaataccg ccgtttttca
    25501 gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa acgagccagc ttatctttca
    25561 cccattcgcc aagatcaacg gtttgctggt catactccac caccgctacc ggccgatgac
    25621 caaactcctt gtcggcaacg gaaacgataa acacctgcaa aaccgcagga tgtgcagcaa
    25681 taacacgctc gacttcttcc ggctgaatac cctcaccgcc actgaagaac agattgtcta
    25741 aacgtccgac aatggtcagc ttgccattat gcatctcacc gcgatcgcgc gtagcgtacc
    25801 agccttcgtc attaaccagt gaaaccagtt gcccgttacg ccagtaacct tctgccatac
    25861 tggcagcccg cagccacact tcattattaa cgattttcac ttcccgaccc ggcagcggca
    25921 aaccaacgtc tgccaggccg tcggcttctt tcgcacatac cgtggaggca aactcggtca
    25981 gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg
    26041 ggatagccgc gccgccaagt aacaccgctt tcagggaaac ggaactgcgg ttaaccagca
    26101 aacgccagag ttgtgttggc accagtgaag cgtgagtaca gcctgccagc atttgctcca
    26161 atggctgttt atcacgcacc gtcatccgcg caccagcgta taaccagcgc cacataattc
    26221 cctgaccgga gacgtgaaac agcggtaaag agagcaacca gtcatcgtga tcgccaaacg
    26281 gaatcagcga taacacacct tgcgcactgg caagatgggc ctgataagta tggacagcgg
    26341 ctttcggcaa accggtagaa cccgaggtca acgtcattga gcacagacgc gtcggctgcc
    26401 acgcagcggc atgtacgcct tcaaccagct gaatgtgcag cgacgttaat gccggaaacg
    26461 tgttttcccc atccggcacc agcgcaaatt gcagcgtcag attgggcagc aattcttcaa
    26521 gcaacggttg cggcagctga gggttcacgg gcaacacccg cgccccgcat tgcagtaacg
    26581 ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg caacatcacg ccgctgccct
    26641 caaccacccc ctgcgccgca aagcccgtag ctaactcatc gacgcgagca caaagctcgc
    26701 gccagttgag ttgctcgtca ttaagacgta aggcgatggc ttctccccgc acttgccgcc
    26761 agtgacgcca cggccagtca gagaagatca taacaactgc tcctgtacat cagcgtccac
    26821 taacggcagt gggctccccg gccagcgacg aacctgctgc gcctgcatca gatccagcgt
    26881 gtccagtccc ggaatggtgt ctggcgttaa ccaggcagca atccgcgcca gttgcgttag
    26941 acctaagctc gactcaatgg aagaactgat caccgccgtc agccccagcg catgcgccgc
    27001 ctgtacctgc tcgcgtactt tttccagact gcccgtgagc gtgggtttga taactaccgc
    27061 gcgcacgccc tcttcagcca caaaggcaaa atccggctcg cgcaggcttt catcccaggc
    27121 aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc
    27181 ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca tatttggcaa actgctgacc
    27241 tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg gaattgcctc
    27301 cagcaacaga ttcaccacca tgccgtcgcg caccgcttcg tacaatccca ctttgacctt
    27361 cgccactttc tcgcccggca tatcggcaag tttgaggatc agatcgtccg gatcgccatt
    27421 acacagcggt gccgcacggt agttggctgc ttgcggcaac gtatctgtca gttctgccaa
    27481 tgcacagctt acgccaaagg ccacggaagg catctgcggt agctcgcaat cgcctgccag
    27541 ccagttattt acccaggcaa gcagcacgct ttgcgcctct tcccagtttt cctggctgaa
    27601 gcccggcagt ggggagatct ccccccaccc ttcgcgctcg ccctcacgca ggcaaacgta
    27661 cagcccgtcg cgggttttta accgcctgtc gcgcagaacc acccccgcgt ccatggggat
    27721 ctgccagcgg tatacctgcg cgctacgcat tacggattcc gtttaaattt gctgaaatca
    27781 ggctgacgtt tctggttgaa agcgttgcga ccttcctgac cttcttccgt catgtagaac
    27841 agcatggtgg cgttgcccgc cagctcctgc agccccgcct gcccgtcaca gtcggcgttc
    27901 agcgcagctt tcaggcagcg cagcgccatc gggctgtttt gcagcatttc gcggcaccaa
    27961 cggacggttt ctttttccag atccgccagc ggtaccacgg tgttgacaag gcccatatcc
    28021 agcgcctgtt ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc ttttttctgc
    28081 ccgacgatac gagccatgta ggaagcgccc cagccgccgt cgaaggaacc gactttcggg
    28141 ccagtctgac cgaagatggc attatctgct gcgatagtca ggtcgcacat catgtgcaga
    28201 acgtgaccac cgccgatgga gtagccagcc accatcgcga caaccggttt cggacaggta
    28261 cggatctgac gctggaagtc cagcacgttc aggtgatgta cgccggaatc atctttatag
    28321 ccgccgtaat caccacgcac tttctggtca ccaccggagc agaacgcttt atcgcctgcg
    28381 ccagtcagaa tgatcacgcc gatattgtcg tcataacgcg catctgccag tgcctggatc
    28441 atctctttta ccgtcagagg acggaaggca ttgcgcacct gcggacgatt aatggtgatt
    28501 tttgcgatac cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg
    28561 tgccattcaa ccggtgcgta aggcattgct tcatcaggat aaatcatatt gtgtccttta
    28621 gtcagaaacg caagatctgc gccagacttg cgattacgcc agcgggattt tcccgatgcg
    28681 cgttatgtcc ggcgcgagga atgacatggc agtcggcagc cagttccgcc gccagggcgc
    28741 ggaatttgct gtcacgttca ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt
    28801 tagcacgtaa atcaggctgg acggcgagag aagtcgcctc cagcatggcg gcaagggttg
    28861 cgccattatt gttgctgcgc agcgccacca gctcccggcg ttgatcgtca ttgagcgagg
    28921 caaaaacagg ctgttgatac cagtcggcaa ataccgccgt taacggttct gtgcgaaaac
    28981 gctgcgccca ttggcgatcg gaacgctgac gttccgcacg ttgttcagca ttttgcagcc
    29041 ccggatgccc gccttcgaca ataaccccac aaagccccgc cagcccctgg caagccgcca
    29101 tcatcgccac ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg atgttgtaac
    29161 taaccaaggt tttacgcagt aagtcggtga catcatcaaa tccatcgacg ctaatcgccg
    29221 ccgaaccacc atgacctagg agatcaacat acaaccgtga gtagtcggca aacgcctcgc
    29281 ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc agccagggta
    29341 aacctagttt tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta cctgcgccag
    29401 aagttgctgg agcgtttgcg caccatcggt gtcgttaacc accatttcaa tcaccgtggt
    29461 ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt tttgcggatg
    29521 atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag
    29581 atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt gcccgccgtt
    29641 gttgttcacc acaattaata ccagcggcgc agaaacctga cgcaataacg ccagcgcgtt
    29701 gagatcgtaa agcgcggaga gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg
    29761 ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc ggttgctgta
    29821 caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt
    29881 accaacaaac aattgcccct gttcaggcag atagtcgctg atgcgatgcg ccagttgcgc
    29941 ttcgccaaac gcatcacggc gggcaataac cgcctgcatt gcctgttccg ccaggcgcgg
    30001 gatttcaacg caccagggct ggcgtttttc tgccggatgc agctccagcc agtcggcaat
    30061 attggcaatt aagcgacgtc cgcgatggtg tgccggatca agtcgccctt caatgtcatc
    30121 aacaatccag tactcttctg gttcacagct tgcctgccat tgcaggagcc gtttccccgt
    30181 caggctactt cccagttgca ccacaatttg cgcctgctgc agctcgctgg tcgctttggc
    30241 attgcctaac caaagatcgg cacacggcag cggctgcccg gtttgtgaca gcacatcgcc
    30301 aatcagcggc cagccaagag tttgcgccca cagggcaact tttttgccct cttccgcact
    30361 catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt
    30421 ttcactttcc agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc accagtcacc
    30481 cagacgctgt tgccagctaa gcccggtatc gtccatttcg ccatacagcg gttcagcaaa
    30541 cgggcagttg atatggactc cccccgcatg aagcgtaccg agagcgtggt cgatggtaga
    30601 aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac tgtgcgtggg
    30661 gtgagaggcg aacattcccg gctggcgaat tgcctgattc gcgccgcagt caattagctc
    30721 cggcgggcga tcggcggtta agagaatcag tttttctccg gttaacccgg cttcaatcag
    30781 tgccggatag agatttgcca ccgccgtgcc ggaggtcaca atcaccgcca ccggctgctt
    30841 gctgactttc gccagcccca gcgccagatg ccccaaccca cgctcatcga aatgggtgtg
    30901 gtgaatgaat gcggaattct ccgccgccgc taacgttaac ggtgtagaac gcgagcctgg
    30961 ggcgatacag atgtgtctga cgccgtgacg cgttaatgct tccagaatga ccgccgccca
    31021 gcgtcggtta aatgcgctta ctgacatgag tttgtccggt atcaatattg cggctaagta
    31081 taaggagcta caaaaataga ttattgatat gaatcggtaa tgatgcgact cattactatt
    31141 ccatttgtaa taaagtacgc agccctgccg ctttgttgtc gatttcctgc cactcttgct
    31201 cggggtcgga accacggaca atgcccgcgc cagcatataa tcgcacgaca ttgccgctaa
    31261 tttttgctga gcgcagggaa acgcagaatt cgctttgttg taatgagaga tagcccgcag
    31321 aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc gataaactgt cgcgccagat
    31381 cgcgcggtaa gccagcaact gctgccgttg gcagcaactg atgtaaacag atcacatcat
    31441 ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg ctgcacttta cgcagacgca
    31501 gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt tggcagatat
    31561 cttccaccac cagcatgttc tcgcgctggt ttttatcatc cgccatcagc cactctccta
    31621 actgctgcgc ctgcttatca tcaggatgat ttgctactgt tcccgccagc gcttcagtac
    31681 gcagcgcttt gtcacgccgc cgccataacc gttccggtga agagccaaga aaagcatttt
    31741 cgccatcaaa ggccatgtaa aaatggtagc aattcagatt cagtcgacga ctggcagcca
    31801 tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc agttgcccga gcgagcacca
    31861 ctttatcgag ctcaccttcg gcgatggttt tcgttgccag ttcgattaat tgcgtccagc
    31921 ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa atgtaaccca ggcaagggct
    31981 tgatactcac cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc tgaagggagc
    32041 tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc cattccaggc
    32101 ggggtaaaag taaattgccc tgcgacgggt caaaagcatt cagcccccaa atgcgtaagt
    32161 cagcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg
    32221 taatcgcgcc caggacggca gcttcttcat caccattacg ttgttgccag tagaattgcg
    32281 ggtacgtttg ctgactggcc agccagctca aggcatcaaa agcgtctttg agagggaaag
    32341 gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta
    32401 gattttccag cgccgtagta agtgattgca cgcggacctc tccctgttaa aagcctcaca
    32461 ttatacgggg tactacaaaa aaaatgcagt accccggtgt agggaggttt taacggcgtg
    32521 ccagcaacag tcctagtacc agcccgacgg ccgcgcccac accaattcct tgccagggtt
    32581 tttcgtggac gtagtcatca gcacgataaa cagcctgctt cgcccgatag taataactgt
    32641 ctgaagcctg gctaacccgt tttttcacat catccagcgc tttttctgca tgcgctttca
    32701 gctcaacata tttctgatcg gcgggatcgc cagaggagcg gagcacctct tccagtgttt
    32761 cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta gacattctcg
    32821 ttctccattt gcgtaaaacc tgtcactaac tatagacaag agttgttgat tacgcctgaa
    32881 ttacctcgcg tgccatccca atgtgtggga taccatcttc ttcatagacc tccgtcacag
    32941 ggataaaacc gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg aggtaaacag
    33001 gcttatcagg ccagtgatgc gtacatgttt cgagtgtttt actcatcagc tgctgcccca
    33061 ctttttcacc acgcaacgct tcactgacaa tcacccgacc tataacgacc ggctcaagat
    33121 catcatcact tttcagaatc ctcgcatacg ccaccagttc atcatttttc caaccgagaa
    33181 tatggcgatt atcccccgtc aaatcatcac catcgatatc ctgataaggg caattctgct
    33241 cgacaacaaa caccgcgcaa cgcagttgta ataaggcata aagctgggaa accgataatt
    33301 cagagtgatg cagatcttgc cattcaatca taatgctctc cttttagatg tatgccttat
    33361 actagccgga atattttttg aaacgtgatg aactcatgga attaattttt ttaggtactt
    33421 cagccggtgt gccaacccgc acgcgcaatg tcacggcaat attgcttaac ctgcaacatc
    33481 cgacccagag cggactttgg ttgtttgact gcggtgaagg cacccagcat cagctactgc
    33541 ataccgcctt taaccctgga aaactggaca agatttttat cagtcacctt catggcgatc
    33601 atctttttgg tttacccggc ttgctgtgca gtcgttctat gtcaggcatt atccaaccct
    33661 taacgattta tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg cggattagcg
    33721 gctcatggac cgattatccg ctggaaattg tcgaaattgg cgctggcgaa attctcgatg
    33781 atggcctgcg caaagtaacc gcttatccgc tggaacaccc actggaatgt tatggctatc
    33841 gtattgaaga acatgataaa ccgggtgcat taaatgccca ggcattaaaa gctgctggcg
    33901 tgccgcctgg cccactgttt caggaattaa aagcgggcaa aacaatcacg ctggaagatg
    33961 gaaggcagat taacggcgca gattacttag ctgctccagt gccaggtaaa gcgctcgcta
    34021 ttttcggcga taccggcccc tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca
    34081 tggtgcacga agcgacgctg gatataacca tggaagccaa agccaatagt cgcggccata
    34141 gctctacacg ccaggctgcg acactagccc gtgaggctgg agtcggcaag ctaatcatta
    34201 cccacgtcag ctcgcgctat gatgacaaag gttgtcaaca cctgttacgt gaatgcaggt
    34261 caattttccc ggcgactgaa ctggcgaatg atttcaccgt gtttaacgtt taactctgct
    34321 tattattaaa aacagggtga aacttgccct gttatagcaa cccgcgctac atatatccac
    34381 ccacacaggc atattgagcg atttttgata tttattcatc tccttacgca ctatattcag
    34441 agcaataagt tgcctctttt catcttgatt cagcacaacc tttgcaacta aaaggagccc
    34501 atgatggtta cagttgtcag caattattgt caattatctc aaacccaact cagtcagaca
    34561 tttgcagaaa aatttactgt gaccgaggaa ttactgcagt ctttaaaaaa aacagcgtta
    34621 tccggagatg aagaaagtat tgagttactg cataatattg cgttaggtta tgatgaattt
    34681 ggaaaaaaag ctgggtaatg ctgccaactt ac
//
LOCUS       DAJTYH010000035        34535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTYH010000035 DAJTYH010000000
VERSION     DAJTYH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO609_001666"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO609_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6633122.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO609_001667"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO609_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6633123.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO609_001668"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO609_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6633124.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO609_001669"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO609_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6633125.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO609_001670"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO609_001670"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6633126.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO609_001671"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO609_001671"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6633127.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO609_001672"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO609_001672"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6633128.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO609_001673"
     CDS             complement(4598..5320)
                     /locus_tag="OO609_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6633129.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO609_001674"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO609_001674"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6633130.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO609_001675"
     CDS             6520..6738
                     /locus_tag="OO609_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6633131.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO609_001676"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO609_001676"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6633132.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO609_001677"
     CDS             complement(7472..7672)
                     /locus_tag="OO609_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6633133.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO609_001678"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO609_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO609_001679"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO609_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6633134.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO609_001680"
     CDS             10101..12014
                     /locus_tag="OO609_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6633135.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO609_001681"
     CDS             12014..13036
                     /locus_tag="OO609_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6633136.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO609_001682"
     CDS             13030..13248
                     /locus_tag="OO609_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6633137.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO609_001683"
     CDS             13302..14171
                     /locus_tag="OO609_001683"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6633138.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO609_001684"
     CDS             complement(14226..14630)
                     /locus_tag="OO609_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6633139.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO609_001685"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO609_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6633140.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO609_001686"
     CDS             15615..17705
                     /locus_tag="OO609_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6633141.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO609_001687"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO609_001687"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6633142.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO609_001688"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO609_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6633143.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO609_001689"
     CDS             complement(19673..20275)
                     /locus_tag="OO609_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6633144.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO609_001690"
     CDS             complement(20265..20432)
                     /locus_tag="OO609_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6633145.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO609_001691"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO609_001691"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6633146.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO609_001692"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO609_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6633147.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO609_001693"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO609_001693"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6633148.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO609_001694"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO609_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6633149.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO609_001695"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO609_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6633150.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO609_001696"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO609_001696"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6633151.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO609_001697"
     CDS             28269..28436
                     /locus_tag="OO609_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6633152.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO609_001698"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO609_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO609_001699"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO609_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6633153.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO609_001700"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO609_001700"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6633154.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO609_001701"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO609_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6633155.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO609_001702"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO609_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6633156.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..>34535)
                     /locus_tag="OO609_001703"
     CDS             complement(33764..>34535)
                     /locus_tag="OO609_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR6633157.1"
                     /translation="VIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQD
                     SYVLADVLATLRAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVV
                     GDADVISRIRATLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAV
                     AEVKSAVIANAQSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLS
                     GTFRQANPQLEHCAIRINPNRSGEETVLRILRESIASI"
BASE COUNT         8299 a         9272 c         8757 g         8207 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacc
//
LOCUS       DAJTYH010000036        34435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTYH010000036 DAJTYH010000000
VERSION     DAJTYH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..322)
                     /locus_tag="OO609_001704"
     CDS             complement(<1..322)
                     /locus_tag="OO609_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633158.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIEN"
     gene            692..1642
                     /gene="corA"
                     /locus_tag="OO609_001705"
     CDS             692..1642
                     /gene="corA"
                     /locus_tag="OO609_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6633159.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1683..2063)
                     /locus_tag="OO609_001706"
     CDS             complement(1683..2063)
                     /locus_tag="OO609_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6633160.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2077..2493)
                     /locus_tag="OO609_001707"
     CDS             complement(2077..2493)
                     /locus_tag="OO609_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633161.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2552..3442)
                     /gene="rarD"
                     /locus_tag="OO609_001708"
     CDS             complement(2552..3442)
                     /gene="rarD"
                     /locus_tag="OO609_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6633162.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3494..3961)
                     /locus_tag="OO609_001709"
     CDS             complement(3494..3961)
                     /locus_tag="OO609_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6633163.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4126..4995
                     /gene="pldA"
                     /locus_tag="OO609_001710"
     CDS             4126..4995
                     /gene="pldA"
                     /locus_tag="OO609_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6633164.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5128..6957
                     /gene="recQ"
                     /locus_tag="OO609_001711"
     CDS             5128..6957
                     /gene="recQ"
                     /locus_tag="OO609_001711"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6633165.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7021..7641
                     /gene="rhtC"
                     /locus_tag="OO609_001712"
     CDS             7021..7641
                     /gene="rhtC"
                     /locus_tag="OO609_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6633166.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7703..8323)
                     /gene="rhtB"
                     /locus_tag="OO609_001713"
     CDS             complement(7703..8323)
                     /gene="rhtB"
                     /locus_tag="OO609_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6633167.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8434..9456
                     /gene="pldB"
                     /locus_tag="OO609_001714"
     CDS             8434..9456
                     /gene="pldB"
                     /locus_tag="OO609_001714"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6633168.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9464..10264
                     /gene="yigL"
                     /locus_tag="OO609_001715"
     CDS             9464..10264
                     /gene="yigL"
                     /locus_tag="OO609_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6633169.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10340..11239
                     /gene="bioP"
                     /locus_tag="OO609_001716"
     CDS             10340..11239
                     /gene="bioP"
                     /locus_tag="OO609_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6633170.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11127..12080)
                     /gene="metR"
                     /locus_tag="OO609_001717"
     CDS             complement(11127..12080)
                     /gene="metR"
                     /locus_tag="OO609_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6633171.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12316..14577
                     /gene="metE"
                     /locus_tag="OO609_001718"
     CDS             12316..14577
                     /gene="metE"
                     /locus_tag="OO609_001718"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6633172.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14616..15431)
                     /locus_tag="OO609_001719"
     CDS             complement(14616..15431)
                     /locus_tag="OO609_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6633173.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15693..16454
                     /gene="udp"
                     /locus_tag="OO609_001720"
     CDS             15693..16454
                     /gene="udp"
                     /locus_tag="OO609_001720"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6633174.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16595..18022
                     /gene="rmuC"
                     /locus_tag="OO609_001721"
     CDS             16595..18022
                     /gene="rmuC"
                     /locus_tag="OO609_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6633175.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18117..18872
                     /gene="ubiE"
                     /locus_tag="OO609_001722"
     CDS             18117..18872
                     /gene="ubiE"
                     /locus_tag="OO609_001722"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6633176.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18886..19491
                     /gene="ubiJ"
                     /locus_tag="OO609_001723"
     CDS             18886..19491
                     /gene="ubiJ"
                     /locus_tag="OO609_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6633177.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19488..21128
                     /gene="ubiB"
                     /locus_tag="OO609_001724"
     CDS             19488..21128
                     /gene="ubiB"
                     /locus_tag="OO609_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6633178.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21207..21476
                     /gene="tatA"
                     /locus_tag="OO609_001725"
     CDS             21207..21476
                     /gene="tatA"
                     /locus_tag="OO609_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6633179.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21480..21995
                     /gene="tatB"
                     /locus_tag="OO609_001726"
     CDS             21480..21995
                     /gene="tatB"
                     /locus_tag="OO609_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6633180.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21998..22774
                     /gene="tatC"
                     /locus_tag="OO609_001727"
     CDS             21998..22774
                     /gene="tatC"
                     /locus_tag="OO609_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6633181.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22816..23598
                     /gene="tatD"
                     /locus_tag="OO609_001728"
     CDS             22816..23598
                     /gene="tatD"
                     /locus_tag="OO609_001728"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6633182.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23595..24083)
                     /gene="rfaH"
                     /locus_tag="OO609_001729"
     CDS             complement(23595..24083)
                     /gene="rfaH"
                     /locus_tag="OO609_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6633183.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24250..25743
                     /gene="ubiD"
                     /locus_tag="OO609_001730"
     CDS             24250..25743
                     /gene="ubiD"
                     /locus_tag="OO609_001730"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6633184.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25789..26490
                     /gene="fre"
                     /locus_tag="OO609_001731"
     CDS             25789..26490
                     /gene="fre"
                     /locus_tag="OO609_001731"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6633185.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26682..27845)
                     /gene="fadA"
                     /locus_tag="OO609_001732"
     CDS             complement(26682..27845)
                     /gene="fadA"
                     /locus_tag="OO609_001732"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6633186.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27855..30044)
                     /gene="fadB"
                     /locus_tag="OO609_001733"
     CDS             complement(27855..30044)
                     /gene="fadB"
                     /locus_tag="OO609_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6633187.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30234..31565
                     /gene="pepQ"
                     /locus_tag="OO609_001734"
     CDS             30234..31565
                     /gene="pepQ"
                     /locus_tag="OO609_001734"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6633188.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31565..32179
                     /locus_tag="OO609_001735"
     CDS             31565..32179
                     /locus_tag="OO609_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6633189.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32218..33669
                     /gene="trkH"
                     /locus_tag="OO609_001736"
     CDS             32218..33669
                     /gene="trkH"
                     /locus_tag="OO609_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6633190.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33681..34226
                     /gene="hemG"
                     /locus_tag="OO609_001737"
     CDS             33681..34226
                     /gene="hemG"
                     /locus_tag="OO609_001737"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6633191.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8107 a         8694 c         9300 g         8334 t
ORIGIN      
        1 cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg ccgttcatcg
       61 ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac gttccccacg
      121 cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga ttaacggtat
      181 acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca acagcaaaca
      241 agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc attcctttac
      301 caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta tcctgttagc
      361 gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg ttctgttgcg
      421 tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa gaggacattc
      481 gccttggaca cacccagtag atactggctc actatcctgt catccaggat caactcctaa
      541 ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt ttcccgtcgc
      601 gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc caccgaactg
      661 tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac tggaaaataa
      721 ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag tatggattga
      781 tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg gccagagcct
      841 ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg aagacgacga
      901 cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg ccggtaactc
      961 cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc gtgaactgcc
     1021 cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg gtaacgccta
     1081 cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg aaattgaaaa
     1141 tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc agggcgatga
     1201 gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga aagttcgcct
     1261 gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc gtttaccggg
     1321 tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc tgccgcataa
     1381 cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt ttatcaacat
     1441 cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc tgccgccaac
     1501 gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga agtggagttt
     1561 cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt atctgtactt
     1621 taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa gccacctgtt
     1681 attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca tttgttcgtg
     1741 ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat taatgcacca
     1801 acaaatagag atagaatgcc aagtaataga actataattg cttttttcca taagcccaga
     1861 acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat agttaatcgt
     1921 tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc ccagaatcca
     1981 ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact taatgaatca
     2041 tcgttcatat attccttact cataaatact ccataactat tgttttgatg aatcagtagg
     2101 tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca tcgaaccatt
     2161 gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat atactgctaa
     2221 tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc gttggcaatt
     2281 catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa aacctgcttc
     2341 cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa taaataaaaa
     2401 tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac gtaacataaa
     2461 ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct catacaagag
     2521 tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc tgcgtataaa
     2581 tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc accatcttat
     2641 cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc gtcgggccaa
     2701 tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca gtaaaacaca
     2761 acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc gacatcgggt
     2821 tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc gccgccacgg
     2881 gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca atcttcttgc
     2941 gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata ggtagcgaac
     3001 caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc agccattgca
     3061 tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc agcgggttaa
     3121 taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag ataaacagta
     3181 gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc tgtggcgtct
     3241 gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc accataaaga
     3301 aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag taaatcagct
     3361 tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt aatacgccct
     3421 gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca gcagtttacc
     3481 tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata taaagctgtt
     3541 cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc aacaggctac
     3601 tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga agatcaatgg
     3661 tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg tggcgggtta
     3721 aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca atgactccgc
     3781 cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta aaggccagct
     3841 gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta aatggcatgt
     3901 gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt acggcagaca
     3961 tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt tgttgttggt
     4021 ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa tagcccgcag
     4081 aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg gactctgcag
     4141 ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga ggcaacggtg
     4201 aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat gctgcaggag
     4261 catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta cacccaaacc
     4321 agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc gcgtaaggat
     4381 gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt aggcccaaac
     4441 tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa tagcgaagag
     4501 tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt tgccaccgat
     4561 taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca cgactctaac
     4621 gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct gatggcagaa
     4681 aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac tgacgataac
     4741 ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca cctcggtgat
     4801 gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg cgcggagtta
     4861 ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta cagcggctat
     4921 ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg ggttatgcta
     4981 aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag catccaggat
     5041 taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg
     5101 tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa tctggagtcc
     5161 ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc cggccaggaa
     5221 gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc cactggtggc
     5281 ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac cgtggttgtt
     5341 tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa cggcgtggcg
     5401 gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat gacaggctgc
     5461 cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct ggataacttt
     5521 cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc gcactgtatc
     5581 tcccaatggg gtcacgattt ccgcccggaa tatgccgcgc tcggtcagtt gcgccagcgg
     5641 ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac gcgccaggat
     5701 atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg
     5761 aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat gcgctacgtg
     5821 caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa agtagaagac
     5881 accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc cgggctggaa
     5941 aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca aattgtggtg
     6001 gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt ggtccacttt
     6061 gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg gcgtgatggc
     6121 ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct gcgccgttgt
     6181 ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact caatgcgatg
     6241 ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta ttttggcgaa
     6301 gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa acagtacgac
     6361 ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca gcggtttggg
     6421 atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg cgactatggt
     6481 catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca ttgggtgagc
     6541 gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca gcattctgcc
     6601 ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt gcaacttgcc
     6661 gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat
     6721 gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga tgaaagtaat
     6781 gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga acagatgccg
     6841 atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct ggaacgcttt
     6901 ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga agagtagtca
     6961 gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg agtcaaatgt
     7021 atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat gagccccggt
     7081 cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga agcgatgatg
     7141 ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt gcttggcctg
     7201 catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg cggtggcctg
     7261 tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga ggctgtatct
     7321 gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa aggcttactg
     7381 accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc attgtttgtc
     7441 ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat tgtcgaaaca
     7501 ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg ccgtggttat
     7561 caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg atttggcatt
     7621 catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga taaggcgttt
     7681 acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc gctcccacca
     7741 gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt cctttaatcc
     7801 atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata tcgaccacaa
     7861 tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc atgatgaatt
     7921 gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc acaaaaactg
     7981 cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga tcaattgcgc
     8041 cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct gcccacttta
     8101 acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg ccaaccagca
     8161 caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg ccgcgataac
     8221 cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac aggcttaaaa
     8281 tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac tcccggtgtg
     8341 tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg cttaaaaaaa
     8401 cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa agactgggaa
     8461 acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt ctggcgtcag
     8521 cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt ccgttttcgc
     8581 gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag ctacgtaaaa
     8641 tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat catcgaccat
     8701 cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca tgttaatcgc
     8761 tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca gcccggtccg
     8821 tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac attatttctg
     8881 caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt tgggatcgtg
     8941 attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc gcatccacgt
     9001 ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt tgctatcaac
     9061 gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc tgatgaccca
     9121 acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct ggctggcgaa
     9181 caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca agctgaagag
     9241 gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac cgccgcgggc
     9301 catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga gatccttttt
     9361 gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt caacaggcat
     9421 aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc aggttgttgc
     9481 gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt acgccaaaga
     9541 aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg gtcgtcacca
     9601 cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga ttacctccaa
     9661 tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc tggatcgcga
     9721 cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta ctaacgttta
     9781 tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct tttttaaaga
     9841 agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag gcatcagcaa
     9901 agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg cgattaacgc
     9961 tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg aagtgatggc
    10021 gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg gctacagcct
    10081 gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt cgatggcggg
    10141 gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc cggagctgga
    10201 agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac tctatttatc
    10261 gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg gatactcgtt
    10321 aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt gggccttctc
    10381 ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg cggtgctggt
    10441 gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta gcaatagcct
    10501 gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga tgtatatgct
    10561 gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca ccgtactgac
    10621 gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc gctggggcta
    10681 tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg atcaggtcac
    10741 cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt ttgccatcgg
    10801 catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata acgcctttgc
    10861 gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc tgggaaatgc
    10921 gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg gcgtggtggc
    10981 ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg ccggaacgct
    11041 gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg ctatctggca
    11101 ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg cctcactgtg
    11161 ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc gcaggcgtga
    11221 ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc atcgcgcacg
    11281 gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac cagaccctgg
    11341 cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg cgcggcaacc
    11401 atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac gcctgccggc
    11461 tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat taatagcgtc
    11521 tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg gtcaggtgct
    11581 aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact gcgcggcagg
    11641 atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg cgggtcaaac
    11701 gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa atttcctaaa
    11761 gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag acgcgtctgc
    11821 tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg gtttgccagt
    11881 tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg cacaaatagc
    11941 cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga ttgcgtctga
    12001 tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg tagcgttttc
    12061 aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt gcgcttgagg
    12121 aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa atcattcgtc
    12181 ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact tcagtaagct
    12241 cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga tttacatata
    12301 attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg cgttggcctg
    12361 cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac gcgtgaagaa
    12421 ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa gcaagcgggt
    12481 atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac caccagtttg
    12541 ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga tatcgacacc
    12601 ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc agcggaaatg
    12661 accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa aggccaacag
    12721 ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc gctgagccac
    12781 aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa agtgaaaggt
    12841 gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca gcaagtgctg
    12901 gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc gctggtactg
    12961 gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct ccagggacag
    13021 gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga cacgattact
    13081 gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa cgttgttgaa
    13141 ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa tggtcgtaac
    13201 gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt cggcaaacgt
    13261 gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct gagcgtggaa
    13321 acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa atgccatgaa
    13381 ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc agagtggagc
    13441 gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt agaaaagcgt
    13501 ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt gcgtgctgaa
    13561 gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg ttccttcccg
    13621 caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct cgacgccaac
    13681 aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca ggaacgtttg
    13741 ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga atactttggc
    13801 gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta cggttcccgc
    13861 tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat taccgtggag
    13921 tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct gacggggccg
    13981 gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac catcgccaaa
    14041 cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg aattggcatc
    14101 atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag cgactgggat
    14161 gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc gaaagatgac
    14221 acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga ttcgattgcg
    14281 gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga gttgctggag
    14341 tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga cattcactcg
    14401 ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc aaaacgcatt
    14461 ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg ctggccagaa
    14521 acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg ggggtaaatc
    14581 caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt cccaccatac
    14641 tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc atgatagctc
    14701 gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat ctctgctttc
    14761 gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg aatgctgtta
    14821 tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat atcaacaggt
    14881 tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc cactgcggct
    14941 tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca gcagaatccg
    15001 gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc gacatgatcg
    15061 agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag caacgtgggg
    15121 atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc gatagccaga
    15181 tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt
    15241 tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct ggcatggtaa
    15301 gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc
    15361 ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc agattgttgt
    15421 gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc
    15481 agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga
    15541 agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac gcatttgcgt
    15601 catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct ttttgcttct
    15661 tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt tttcatctcg
    15721 gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc gacccggatc
    15781 gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct caccgcgaat
    15841 tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct accggtatcg
    15901 gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt cgcaccttcc
    15961 tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat gtcctggtta
    16021 ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg gaattcccgg
    16081 ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct attggcgcga
    16141 caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag gaacgttacg
    16201 atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag tggcaggcga
    16261 tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt gcaagtcagg
    16321 gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa gagatcccga
    16381 atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg gaagcggcgc
    16441 gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc ttttgtattt
    16501 ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc acaattctat
    16561 tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca tggtttacgc
    16621 agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc aacatgcgca
    16681 gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa gcgcggcaaa
    16741 acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca ataacgaagt
    16801 gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag taaccacgcg
    16861 gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta acagcgagca
    16921 gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca gcaatcgccg
    16981 ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac gtgaacaact
    17041 ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag aacgccatac
    17101 cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc aggaagcgat
    17161 caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg gcgaggtagt
    17221 attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg aaacccaggt
    17281 cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc ggctgccgca
    17341 gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac gctattttaa
    17401 cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt cggtgcgtaa
    17461 ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc gaactctgga
    17521 ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg accgccagcc
    17581 ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga ctacgctgct
    17641 ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc gtaacgccca
    17701 gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca tcgatgacat
    17761 gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg caatgaaaaa
    17821 actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg gtttaggagt
    17881 agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc aggatgaaga
    17941 gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac gcgatgatga
    18001 atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga aatctagggc
    18061 atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc attgagatgg
    18121 tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag gaacaaaaag
    18181 cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc atgaatgatt
    18241 tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc agcggcgtac
    18301 gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca gcgaaattct
    18361 cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa tccatgctca
    18421 aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag tatgttcagg
    18481 cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc atttcgtttg
    18541 gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc gtgctgaaac
    18601 ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg ctgagcaaag
    18661 cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc gcgaacgacg
    18721 ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag gataccctga
    18781 aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg acggcagggg
    18841 ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc ttttaaacct
    18901 ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc acccgcgctg
    18961 aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa aggcttttcg
    19021 acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga atgggcaggc
    19081 gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg cgatcgccag
    19141 cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat tcaggtggtg
    19201 caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact gctggcccct
    19261 tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg cgcaaagttc
    19321 ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac tgaagagtgg
    19381 cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc tgccgtcgag
    19441 cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg acgccaggtg
    19501 aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt gatgaactga
    19561 tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta ttctggatgc
    19621 caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg caagaactgg
    19681 ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt tttccaccgc
    19741 atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat ggcaagctgg
    19801 cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg tttgacgatt
    19861 ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg cgattgaaat
    19921 cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg gtcattaaag
    19981 cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg ccggatggtc
    20041 gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt gatgaactga
    20101 atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa gacagcccga
    20161 tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg gtgatggagc
    20221 gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc actaacatga
    20281 aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc gacagctttt
    20341 tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg gaaaacccga
    20401 aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat aaacgctatc
    20461 tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca gagctacacg
    20521 tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt gccattcgta
    20581 cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga catgtactgt
    20641 taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa ctggtgttac
    20701 tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg cagctcgatt
    20761 tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc ggtattcctg
    20821 ctctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg ccagaactgc
    20881 ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt gttgataaga
    20941 ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat tttctcggaa
    21001 ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct gagtgggggc
    21061 tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt tggcgcaaaa
    21121 cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg ctttgtttaa
    21181 tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag ttattgatta
    21241 ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc ggttccgatc
    21301 ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca aagcaggata
    21361 aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag gcggatacga
    21421 atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag gtgtaatccg
    21481 tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc gtcgttctgg
    21541 ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc gcgttgcgtt
    21601 cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag gagtttcagg
    21661 acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa ctgaaagcgt
    21721 cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt gcaaacgatc
    21781 ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa gataatgaag
    21841 ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg ccggaacaga
    21901 agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa ccgaaaaccg
    21961 ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag atactcaacc
    22021 gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta tcgcggtgat
    22081 cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg tatccgcgcc
    22141 attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg cctcgccgtt
    22201 ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc cggtgattct
    22261 ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc gcctggtggt
    22321 gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg cctactttgt
    22381 ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg tgcaggtatc
    22441 caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt ttggtgtctc
    22501 ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct cgtcagaaga
    22561 cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga tgttgctgac
    22621 gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc tgtttgaaat
    22681 cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag aggaaaacga
    22741 cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca gggcggttgt
    22801 catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca atttgcgaaa
    22861 gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct actcatcacc
    22921 ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta ttcgtcctgt
    22981 tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc gactgaagaa
    23041 gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg tggtctcgac
    23101 tttaaccgta acttttcgac gccggaagag caggaacgcg cttttgttgc tcagctacgc
    23161 attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca cgagcggttt
    23221 atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca ttgctttacc
    23281 ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg cattaccggt
    23341 tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt gattccggcg
    23401 gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct cacgccaaag
    23461 ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg tattgcgcac
    23521 tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt caaaacactg
    23581 tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg cttaatctct
    23641 ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc ggtgaaaatg
    23701 gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg ataagggggg
    23761 gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac cgccgatggg
    23821 actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt agcgctgatg
    23881 gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg gaataacggt
    23941 tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat cggagcgagg
    24001 caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc gcgcttgcag
    24061 tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc gcaaggatag
    24121 caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt cagagttcac
    24181 gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg cgcagattaa
    24241 gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac gctgcttgaa
    24301 cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga aatcactgaa
    24361 attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa ccctaaaggc
    24421 tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc gatgggcatg
    24481 gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt tctgaaagag
    24541 ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt taagcaggtg
    24601 ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat cgtctctggc
    24661 gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga tgccgcgccg
    24721 ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca gaatctgggc
    24781 atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct gtcgcatcgc
    24841 ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg tttcccggtt
    24901 tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc cgttccggat
    24961 acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga agtagtaaag
    25021 tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga agggtatatc
    25081 gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta ctataatgaa
    25141 gtcgataatt tcccggtgtt taccgtgacg catattaccc agcgtgaaga tgcgatttac
    25201 cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt ggcgttgaac
    25261 gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt ttacctgccg
    25321 ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta cgccggacac
    25381 gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta cactaaattt
    25441 gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat ttgggcgatt
    25501 accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc tattgattat
    25561 ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga tgccacgaat
    25621 aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga tccagatgtt
    25681 gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg taaaagcgcc
    25741 tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat gacaacctta
    25801 agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt ccgcatcgtg
    25861 ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat ggatgagcgc
    25921 gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat cgagctgcat
    25981 attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat cctcaaagat
    26041 catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga tgaagagcgt
    26101 ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat tttgctgaca
    26161 gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg tgaagagcag
    26221 catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg tctgcaagtg
    26281 gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac cgtgttaacg
    26341 gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc cggacgtttt
    26401 gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg ggaagatcgc
    26461 ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc tgacaggcgg
    26521 gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca tccggcaatt
    26581 ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg gtgccggatc
    26641 ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg ctcaaacacc
    26701 gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg aacgtctttg
    26761 cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc cagcggatga
    26821 cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc cattagcccg
    26881 agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc aaacacgccg
    26941 atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg aaccgggccg
    27001 taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg agcgcgcggc
    27061 ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc tgcgccatcg
    27121 gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa cgccggacgc
    27181 agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta attaaactgc
    27241 ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt aaatgcggcc
    27301 gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca
    27361 cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat gcccgccgct
    27421 ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat cggcacatgg
    27481 cctatatgct ccacgccgcc aaccagacat gcctgcgtat cgccagtcat gatcattcgt
    27541 gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt aaccgccggg
    27601 acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa accctgctcc
    27661 agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc cagcgccggg
    27721 ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg cacgttacga
    27781 aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat gacaacctgt
    27841 tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg ctcaaccgga
    27901 ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac ttcatacagc
    27961 gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc gagggtgtcc
    28021 agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac cagcgccata
    28081 tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac ttcgttgacc
    28141 atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt cggttggctc
    28201 acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt acctttgctg
    28261 tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt ggcatcaaac
    28321 agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc tgctgccatg
    28381 acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata tgccgggccc
    28441 atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc cgcgccgtcg
    28501 cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac aaagaagccg
    28561 gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc gacaactttc
    28621 gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg cattcggtgg
    28681 accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc gttggccagt
    28741 tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg tactttttgt
    28801 tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc ttctaccaca
    28861 atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga gatcacgcca
    28921 gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag tttcgcggct
    28981 tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac cggcacgcct
    29041 ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag cacagcagcc
    29101 tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac atattgatcg
    29161 ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg gacaaaactt
    29221 ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc aatggttttt
    29281 actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat tcctttagcg
    29341 atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg cttcggctga
    29401 cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc catcgcccct
    29461 tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag cgcctgatcc
    29521 gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc cagcatacgt
    29581 ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc cggcagaccg
    29641 atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc gcagccacca
    29701 cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc caggcgatta
    29761 aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac gaggaacagg
    29821 gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga acgcagcagc
    29881 agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag gctggcgacg
    29941 gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc ggcaatgcca
    30001 tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt ctcctgaatc
    30061 cgcaaggtaa tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt aaaaatatgc
    30121 aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac gctctggatg
    30181 aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa aaaatggaat
    30241 cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact cgcgatgcgc
    30301 tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc aacgtttttc
    30361 tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg gtgccggtaa
    30421 ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa ctgtggttct
    30481 atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc tggactgaag
    30541 atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg cctgctgcgc
    30601 gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg ggtattgagg
    30661 ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc tccttcaaaa
    30721 ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat ggtcatcgtg
    30781 cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt gcctatctga
    30841 ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg cttaacgaac
    30901 acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag atgcgcagct
    30961 tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc cgtacctggt
    31021 cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat gaacaactgg
    31081 cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc cagttccatc
    31141 agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt gaagaagcga
    31201 tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat ccgctgggcc
    31261 tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac ctcgcggcac
    31321 cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg gtgttaacca
    31381 tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa gggcagttca
    31441 gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc attcgtatcg
    31501 aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat ctgaaactgg
    31561 cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag agatcaaaaa
    31621 gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga aagcgtttgt
    31681 tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt gggtggcggg
    31741 tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg cgggaacggc
    31801 aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta ccgctgtggt
    31861 agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag cgtatggcgg
    31921 cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat taaccgaata
    31981 tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg ggcagtgtga
    32041 cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg cgcttccggc
    32101 ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt cattgcaatt
    32161 gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc ggcagagatg
    32221 cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc agggaccatg
    32281 attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc ttttacccag
    32341 accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg caaagagaag
    32401 ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac cgtgctgggt
    32461 agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat taccgatgcg
    32521 ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt ggggctggat
    32581 tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg cgggatgggg
    32641 atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat gcagctctat
    32701 cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat tgcggaaacg
    32761 gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct ggcgttgtgg
    32821 tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat cgctattggc
    32881 ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat taacactatc
    32941 attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc actgttaagt
    33001 gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat cggcgtacag
    33061 tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta cagttcggcg
    33121 ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac cgccgggttt
    33181 acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt atgttcagct
    33241 tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg catcctgctg
    33301 ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc cgtctatagc
    33361 attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg gggatttttc
    33421 tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac gggcgtggat
    33481 gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc agggcttggc
    33541 gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct gattgccaac
    33601 atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc gactttctgg
    33661 cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg gacggacaaa
    33721 cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc cagacggatg
    33781 tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt gtggtcattg
    33841 gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc aaaaaacatg
    33901 cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg gcgcgcaaac
    33961 cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg aactcgcaat
    34021 ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt tatcgctggt
    34081 acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg gatacgcgca
    34141 aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa atcgcccatt
    34201 taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg tcagaaaatt
    34261 attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg
    34321 gaacaacggc aaacacgcag ccgggtcagc ggggttctcc tgagaattcc gacagagaaa
    34381 gcaaaaataa atgcttgact ttgtagcggg aaagcgtatt atgaacaccc tgcgc
//
LOCUS       DAJTYH010000037        33853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTYH010000037 DAJTYH010000000
VERSION     DAJTYH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1871
                     /locus_tag="OO609_001738"
     CDS             <1..1871
                     /locus_tag="OO609_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6633192.1"
                     /translation="QQGTPVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPP
                     GSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLG
                     VRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAISASVFRNDDGGKGDMQ
                     QANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGY
                     TCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVF
                     HRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRYSEQDGDQTSWQLSHT
                     FYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFT
                     GTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQ
                     LGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSA
                     SFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLK
                     CPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            1868..2956
                     /locus_tag="OO609_001739"
     CDS             1868..2956
                     /locus_tag="OO609_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6633193.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3044..4342)
                     /gene="exuT"
                     /locus_tag="OO609_001740"
     CDS             complement(3044..4342)
                     /gene="exuT"
                     /locus_tag="OO609_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6633194.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4827..6239
                     /gene="uxaC"
                     /locus_tag="OO609_001741"
     CDS             4827..6239
                     /gene="uxaC"
                     /locus_tag="OO609_001741"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6633195.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6254..7741
                     /gene="uxaA"
                     /locus_tag="OO609_001742"
     CDS             6254..7741
                     /gene="uxaA"
                     /locus_tag="OO609_001742"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6633196.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7824..8375
                     /locus_tag="OO609_001743"
     CDS             7824..8375
                     /locus_tag="OO609_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6633197.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8380..9624)
                     /gene="sstT"
                     /locus_tag="OO609_001744"
     CDS             complement(8380..9624)
                     /gene="sstT"
                     /locus_tag="OO609_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6633198.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10220..11185)
                     /gene="alx"
                     /locus_tag="OO609_001745"
     CDS             complement(10220..11185)
                     /gene="alx"
                     /locus_tag="OO609_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6633199.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11456..12454)
                     /locus_tag="OO609_001746"
     CDS             complement(11456..12454)
                     /locus_tag="OO609_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6633200.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12533..13225)
                     /locus_tag="OO609_001747"
     CDS             complement(12533..13225)
                     /locus_tag="OO609_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6633201.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13302..13805)
                     /locus_tag="OO609_001748"
     CDS             complement(13302..13805)
                     /locus_tag="OO609_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6633202.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            13890..15026
                     /gene="rlmG"
                     /locus_tag="OO609_001749"
     CDS             13890..15026
                     /gene="rlmG"
                     /locus_tag="OO609_001749"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6633203.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15311..15625
                     /gene="higB"
                     /locus_tag="OO609_001750"
     CDS             15311..15625
                     /gene="higB"
                     /locus_tag="OO609_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6633204.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15622..16038
                     /gene="higA"
                     /locus_tag="OO609_001751"
     CDS             15622..16038
                     /gene="higA"
                     /locus_tag="OO609_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6633205.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16083..18101)
                     /gene="fadH"
                     /locus_tag="OO609_001752"
     CDS             complement(16083..18101)
                     /gene="fadH"
                     /locus_tag="OO609_001752"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6633206.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18327..20678)
                     /gene="ygjK"
                     /locus_tag="OO609_001753"
     CDS             complement(18327..20678)
                     /gene="ygjK"
                     /locus_tag="OO609_001753"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6633207.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20695..21114)
                     /locus_tag="OO609_001754"
     CDS             complement(20695..21114)
                     /locus_tag="OO609_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633208.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21092..21445)
                     /locus_tag="OO609_001755"
     CDS             complement(21092..21445)
                     /locus_tag="OO609_001755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633209.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21446..21766)
                     /locus_tag="OO609_001756"
     CDS             complement(21446..21766)
                     /locus_tag="OO609_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633210.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(21900..23333)
                     /locus_tag="OO609_001757"
     CDS             complement(21900..23333)
                     /locus_tag="OO609_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6633211.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23396..23845)
                     /locus_tag="OO609_001758"
     CDS             complement(23396..23845)
                     /locus_tag="OO609_001758"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6633212.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23842..26928)
                     /gene="ebgA"
                     /locus_tag="OO609_001759"
     CDS             complement(23842..26928)
                     /gene="ebgA"
                     /locus_tag="OO609_001759"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6633213.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27111..28094)
                     /gene="ebgR"
                     /locus_tag="OO609_001760"
     CDS             complement(27111..28094)
                     /gene="ebgR"
                     /locus_tag="OO609_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6633214.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28312..28643
                     /locus_tag="OO609_001761"
                     /pseudo
     CDS             28312..28643
                     /locus_tag="OO609_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28685..30064)
                     /gene="ygjG"
                     /locus_tag="OO609_001762"
     CDS             complement(28685..30064)
                     /gene="ygjG"
                     /locus_tag="OO609_001762"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6633215.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30482..32002
                     /gene="aer"
                     /locus_tag="OO609_001763"
                     /pseudo
     CDS             30482..32002
                     /gene="aer"
                     /locus_tag="OO609_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32156..32779)
                     /gene="nfeR"
                     /locus_tag="OO609_001764"
                     /gene_synonym="yqjI"
     CDS             complement(32156..32779)
                     /gene="nfeR"
                     /locus_tag="OO609_001764"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6633216.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33056..33801
                     /gene="nfeF"
                     /locus_tag="OO609_001765"
                     /pseudo
     CDS             33056..33801
                     /gene="nfeF"
                     /locus_tag="OO609_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
BASE COUNT         8258 a         8734 c         8885 g         7976 t
ORIGIN      
        1 gccagcaagg aaccccagta atggttctgc tttcgcgcaa ttctcgtgtt gacgcttatc
       61 gtaatgagca acttttggga tcgttttatc tcaatagtgg ttcgcaattt attgatacca
      121 gttcctttcc gccaggcagc tacagcgtgg cattaaaagt ctacgaaaat aaccaactca
      181 cccgcaccga gcttgtgccg tttaccaaaa ccggcggtct gactgacgga aatgcgcaat
      241 ggttcttaca ggcaggtaaa actacatcac aggtttctga tgatgaaagt tcagcttatc
      301 agctaggagt acgcctgcca ttacatccgc aatatgagct ctacgcaggg ctggcgaatg
      361 ctgatgatgt gagtgctttc gagttaggta ataactggac ggcagattta ggcagggtgg
      421 ggaatcttgc aatcagcgcc agcgtgttcc gtaacgatga cggcggcaaa ggtgatatgc
      481 aacaggccaa ctggagtaat ccgggatggc cgacgttggg cttttatcgg accaactctg
      541 acggcgatgc ttgtacaacc gacagcagag agagctataa cgccttaagc tgttatgaaa
      601 gtatttccgc gacagtttca ctgaattttg tcggctggaa tatgatgctg ggttatacct
      661 gcacacaaaa taacactgat gatagtttgc gttgggataa acagcagagc tttgaaaata
      721 actatcttcg ccagacaaca gcgcaaagta tctctgaaac tgtacaactt agcgcttccc
      781 gcgctattgt gatgcgtgac tggattttga gtacgtcagt cggtgttttc catcgtaatg
      841 acaacggtgg cgataacgac gacaacggct tgtacttatc gttttcgtta tctgacacgc
      901 caacgatgga tagcaataac aacagccatt caaccaatgt gcctacggat tatcgttata
      961 gcgaacagga tggcgatcaa acgtcatggc agttatcgca taccttttat aacgattcat
     1021 tcagccataa agaacttggc gtaaccgtcg gaggcctgaa caccgatacc ataaacagcg
     1081 cggttaacgg gcgttgggat gggcaatacg gaaatgtcta cgctaccgta tctgatagtt
     1141 atgaccgtaa gaatcatgat catctttcgg cctttacggg cacttatagc tctacgctgg
     1201 ctgtcagtcg ctatggcgtt aatttgggag ccagtggtac agacgatttg ctgggtgcgg
     1261 tactggtgga tgtgaaaggc ttctctgaac aggatgaaga gagtcaggat ctgcaactcg
     1321 aagcgcgggt ggcaggcagc cgaactttgc agcttggtca aagtgacagt gtgttgttcc
     1381 cttatcctgg atttcagtct ggttttgttg aggttaacga cagtagccag ggcaatcagc
     1441 aggggacaac aaacatcatt aacggtgcag ggaatcgtga attaatgttg ttgcctggca
     1501 agctgcgcta tcgcgaagtg tctgccagct ttaactacaa ctatatcggt cgtttattac
     1561 tgccggcagc ggtgaaaaaa ttcccgattg tggggctgaa cagcgccatg ttactggtag
     1621 ctgaagatgg cggatttaca cttgaaatta acggcagcga aaaagagctc tatctgcttt
     1681 ccgggcagca attccttaag tgtccactga gtgttgtaaa gaaacgcgcc agcattcgtt
     1741 acagcggaga tgttacttgt agtgtggtga cttattcaca attaccggag tcgattcagg
     1801 ttcaggcaca gttgaaacag cctaaattac gtggaaacgt tcagacggcg caaagggagg
     1861 ttgcaccatg agaaatcgat tgattgcggc gatattgggt ttgtttggca cgctcactgg
     1921 cgttcaggca gctcctgacg tgaccagtga aattacgtat gatttggcat ctggtagagc
     1981 ggattattac ttctggaagg atgaagcgtc ggcaggaaat aatggatata tgtggtatga
     2041 atgttcgtat cctgacctcc aacaaacctg tacagctaat ggaaatatat cgacagtaca
     2101 aatctattta actgaacaac gcagtgggat gcgttggccg gtaaaactca aaggatttaa
     2161 aacagccatt gtaagtagcg atgaagcgcc gctaggatgc aaggggggca aagggcttca
     2221 gacgaatctt aaggattcta atagatcttc atgtacagaa gatggtcaac attattatat
     2281 atacgataca aagtttctta cgctctacct tgagcagaca gagatgaaga atttgccgat
     2341 tggtggcgtc tggaagggga aagttaaatt acattcgaac agcccggccc aggactattt
     2401 cgcaaatatt accctgaata cgctcgaccc caaccatatt gacgtgttct tcccggagtt
     2461 cgcccacgcc acgccaaggg tgcagttaga cttgcatcca acaggaagcg ttaacggcag
     2521 caactacgcg caagatctga ccatgttgga catgtgcctg tacgatggtt ttaacggtaa
     2581 tgccatcagt tatgaaatca tgctcaaaga tgaagggcga ccagctgcag ggcgcagaga
     2641 cggttacttc tctatctatc gtcagggagg gaccaccacc gacgagggag aacgcattga
     2701 ttaccgggtc aaaatgtaca acccggaaac cggtgggcaa attgatgtgc gcaataatga
     2761 aaatatggtc tggaacagca ttaacctgaa acgtgtgcgt ccggtggtac tgcccggtat
     2821 tcgctatgcc gtgatgtgtg tgccgacacc cttgactctg gcagtcgata aattcagcgt
     2881 gatggataaa caggccgggt actacatggg caaattgtca gtaatcttta cgccttcctt
     2941 gccaaccatc aattaaccct gcaaaccgga caaacgcttt tttctatata aacgacgaag
     3001 ccgcctgata aggcggcttc gtcgttataa cttacgttaa acgttaatgt tgcggtgcgg
     3061 gatcgttatg tgtttcctgc gccacctcaa tcgccggttt gttctgcaat acggtccaga
     3121 taaccagcgc acccagcagg tcaaatactg ccagaactgc gaacagcggg ctgaagccga
     3181 tggtgtcagc cagtgcaccg acaaccagcg caaacagcgt acttgccagc catgcggaca
     3241 tcccggttaa accgttcgcc gtcgccactt cgttacgacc gaacacgtca gaagagagcg
     3301 taatcagcgc gccagacagt gcctggtggg caaaaccacc gatacacagc agcataattg
     3361 cgacatacgg gttggtgaac agaccgatca tacccgggcc aatcatcagc acagcaccca
     3421 gcgttacgac catcttacgg gaaacgatca ggttcacacc aaaccaacgc tggaacagcg
     3481 gcggcaggta accaccgagg atacaaccga ggtcagcaaa cagcatcggc atccaggcga
     3541 acatcgcgat ctctttcagg ttaaagccgt aaactttaaa catgaacagc gggatccacg
     3601 cgttaaaagt accccaggcc ggttctgcaa ggaaacgcgg cagtgcgata ccccagaact
     3661 gacggttacg caggatctga ccaacggaca ttttcttcgc cgtgctcacc tggtgctggg
     3721 cttcctgacc attaataata tagtcgcgtt cttcatcggt cagatgcttc tggtcgcgcg
     3781 gatgtttata gaaaatcagc catgccatcg cccagataaa gctcaatgca ccggagatga
     3841 taaatgccat ctgccagctg tgcatcacga ttgcccatac caccagcggc ggcgcaatca
     3901 tcgcaccaat cgaagaacct acgttaaagt agcctactgc gatggaacgc tctttcgccg
     3961 ggaaccattc ggagctggct ttcagacccg ccgggatcat cgctgcttcc gcggcaccga
     4021 ccgcaccacg agcaacagct aggccacccc agctacctgc cagcgcggtt gcaccacaga
     4081 acacggccca cagcacagca aacattgcat aaccgatttt cgtacccagc acatccagta
     4141 cataacctgc taccggttgc atgaccgtat aagcagcaga ataggctgcg atgatatagg
     4201 aatactgttg ggtggagatg tttaactctt ccatcagagt tggcgcagct gccgccacag
     4261 tgttacgcgt caggtaacca agcacggtgc cgagcgtcac cagtgcgatc atataccaac
     4321 gtaacccttt aattttacgc atgtaaaacc tcatcgtttt gttatatccg caggaagcgc
     4381 ggccatcatc ccgccagagg ctgaagatgt tagttacgac cgggtaaccg aaaaaaaaaa
     4441 ccggcacgcc ccgaacgttg ccatgaaagt ttcgtgctaa ttcggcttcc gtaccggtta
     4501 acccgattca tctaacaaat gaatgaatcg gcaatttgca ttccgtcgac ttatgatttg
     4561 cgacggcaga aagataactt gtcatacaac cttaaaaggt gagagccatc acaaatgtgc
     4621 gaatatttgt agggacatta cctgacgaca gcgaggccag tactggcgcg gcctgcagcg
     4681 agatttacca ctttgagagt aattttttta actacgttta ttgatctaac tcacgaaaat
     4741 atcttcggac tctggaaatt ggtgtgataa ctttgtcagc atcgcaccat aagcaagcta
     4801 gctcactcgt tgagaggaag acgaaaatga ctccgtttat gactgaagat ttcctgttag
     4861 ataccgaatt tgcccgccgt ctgtatcacg actacgcaaa agaccagccg attttcgatt
     4921 accattgcca tttgccgccg cagcagattg cggaagacta tcgttttaaa aacctgtatg
     4981 acatctggtt gaagggcgat cactacaaat ggcgcgctat gcgtaccaac ggtgtggctg
     5041 agcgtctgtg tactggtgat gcgtctgacc gtgaaaaatt tgacgcctgg gcggcgactg
     5101 ttccgcacac tatcggcaac ccgttatacc actggacgca cctcgaactg cgtcgtccgt
     5161 tcggtatcac tggcaaattg ctttctccgt caactgccga tgaaatctgg aacgaatgta
     5221 acgaactgct ggcgcaggat aacttctccg cgcgcggcat catgcagcag atgaacgtga
     5281 aaatggtcgg caccaccgat gacccgatcg attctctgga gcatcacgca gagatcgcca
     5341 aagacggctc tttcaccatc aaagtgctgc cgagctggcg tccggacaaa gccttcaata
     5401 tcgaacaggc gacctttaac gactacatgg cgaagctggg cgaagtttcc gataccgaca
     5461 ttcgccgctt tgctgacctg caaactgcct tgaccaaacg tctggatcac ttcgccgctc
     5521 acggctgtaa agtgtctgac catgcgctgg acgtggtgat gtttgctgaa gcgaacgaag
     5581 cggaactgga cagcattctg gcgcgccgtc tggctggcga aaccctgagc gagcacgaag
     5641 tggcacagtt caaaactgcg gtactggtgt tccttggtgc cgaatatgca cgtcgcggct
     5701 gggtacagca gtaccacatt ggcgcgctgc gtaataacaa cctgcgtcag ttcaaactgc
     5761 tggggccgga tgtaggcttt gactccatca acgaccgtcc gatggcggaa gagttgtcta
     5821 agctgctgag caagcagaac gaagaaaacc tgctgccgaa aaccattctc tactgcctga
     5881 acccgcgcga taacgaagtg ctgggcacca tgatcggtaa cttccagggc gaaggtatgc
     5941 cgggcaaaat gcagttcggt tccggctggt ggtttaacga tcagaaagac ggtatggaac
     6001 gtcagatgac ccaactggcg cagctcggtc tgctgagccg ctttgtcggt atgctgactg
     6061 acagccgtag cttcctgtca tacacccgtc acgaatactt ccgccgcatt ctgtgccaga
     6121 tgatcggtcg ctgggtggaa gcgggcgaag caccggcgga catcaacctg ctgggcgaga
     6181 tggtgaaaaa tatttgcttt aacaatgcgc gtgactactt cgccattgaa ctgaactaag
     6241 gtctggggtt gatatgcaat acatcaagat ccatgcgctg gataacgtcg cggtcgcttt
     6301 agcagatttg gctgaaggca cagaagtcag tgtcgataac cagactgtta cgctgcgcca
     6361 ggatgttgct cgtggacata aatttgcgtt aacggatatc gcaaaagggg ccaacgtcat
     6421 caaatatggc ctgccgattg gttatgcatt ggcggatatt gcggcggggg aacacgtcca
     6481 cgcccacaat acgcgcacga atctgagcga tctggatcag tatcgctatc aacttgattt
     6541 tcaggatctg cctgcgcaag cggcagatcc tgaagtgcag atctatcgtc gcgctaacgg
     6601 cgatgtcggg gtgcgtaacg agctgtggat cctgccaacc gtgggctgtg tcaacggcat
     6661 cgcgcggcaa atccagaacc gtttcctgaa agagaccaac aacgccgaag gtaccgacgg
     6721 cgtgttcctc ttcagccaca cctatggctg ctcacaactg ggcgacgatc acatcaacac
     6781 ccgcaccatg ctgcaaaaca tggtgcgcca cccgaacgcg ggcgcagtgc tggtgattgg
     6841 tctgggctgt gaaaacaacc aggttgccgc attccgtgaa acgttgggcg atatcgatcc
     6901 tgaacgcgtt catttcatga tctgccaaca gcaggatgat gagatcgaag ccgggatcga
     6961 gcatttgcat cagctgtata acgtgatgcg caacgataaa cgcgagccag gcaaactcag
     7021 cgaactgaag tttggtctgg agtgcggtgg ttctgacggt ctttctggta ttactgctaa
     7081 cccgatgctg gggcgtttct ctgactacgt gattgctaac ggcggtacta ccgtactgac
     7141 cgaagtgccg gagatgtttg gcgcagagca gttgctgatg gaccattgcc gcgacgaagc
     7201 aacgtttgaa aaactggtca ccatggtcaa cgacttcaaa cagtacttta ttgcccatga
     7261 tcagccgatc tacgaaaacc catcaccggg gaacaaagcg ggcggtatca ccacgctgga
     7321 agacaaatca cttggctgta cccagaaagc gggttccagt gtcgtggttg acgtgctgcg
     7381 ttacggcgag cgtctgaaaa cgccagggct gaacttgtta agtgcgccgg gtaacgatgc
     7441 cgtagcgacc agcgccctgg cgggtgcggg ctgccatatg gtgctgttca gtactggtcg
     7501 cggcacgcca tacggtggat ttgtgccgac ggtgaaaatc gccaccaaca gtgaactggc
     7561 ggcgaagaaa aaacactgga tcgactttga cgcgggtcag ctgatccacg gtaaagcgat
     7621 gccgcagttg ctggaagaat ttatcgacac catcgttgag tttgccaacg gtaagcaaac
     7681 ctgcaacgag cgtaacgact tccgcgaact ggcgatcttc aaaagcggcg taacgctata
     7741 aagtcgaaat gagttgttaa taaaaaacgg cgtttcataa tgtgaagcgc cgtttgcttt
     7801 tccatacctg ctaaggattt atcatgaccg cgtattggct ggcccagggc gtgggtgtca
     7861 tcgcctttct gattggtatc acaacatttt tcaatcgtga cgaacgtcgc ttcaaaaagc
     7921 agctttcggt ctatagcgcc gttattggcg tacatttttt tcttctgggc acctatcccg
     7981 ctggtgccag tgccatcctt aatgccattc gtacattgat taccttacgc acgcgcagct
     8041 tatgggtaat ggcgattttt attgtgctga ctggcggaat tggcctcgcg aagttccatc
     8101 atcctgtcga actattgccg gttatcggta cgattgtcag tacctgggcg ctgttccgct
     8161 gtaaagggct caccatgcgc tgcgtgatgt ggttttcaac gtgttgctgg gtgattcaca
     8221 acttctgggc ggggtcgata ggcggcacga tgattgaggg tagttttctg cttatgaatg
     8281 gcctgaatat cattcgtttc tggcggatgc agaaaagggg aattgatccg tttaaagtag
     8341 agaaaacccc tcccgccata gacgaaaggg gttaaacaat taattacgca gggcgctatt
     8401 tgccagacga tcgtcttccg cctggcaagc tgccgcagtg aacagcacgt cagttgaaga
     8461 gttcagcgcg gtttcgcaag agtcctgcaa tacgccgata ataaagccga cggcaaccac
     8521 ctgcatggcg atatcgttcg aaataccgaa catattacag gccagtggga tcagcagcaa
     8581 agaacccccc gccacgccgg atgcgccaca ggcacacaga gaagccacta cgctcaacag
     8641 cagcgctgtg ggcagatcga ccggaatacc cagcgtatta accgcagcca gcgtcaacac
     8701 ggtaatggtg attgctgcgc ccgccatatt gatggtggct cccagcggaa tagaaactga
     8761 ataggtatcg cgatccagat tcagcttttc acacagcgcc atattcaccg gaatgttcgc
     8821 tgcagagctg cgggtgaaga aggcatacac accgctttcg cgcaggcaca gcagcaccag
     8881 cgggaacggg ttacgacgaa ttttccacca caccagcaat gggttaacca ccagcgccac
     8941 cagtaacata cagccaacca gcacgaccag cagttgcgcg tagccccaca gtgtggagaa
     9001 accggtggtt gccagggtag aagaaaccag cccaaaaata ccgaacggtg cgaagcgaat
     9061 gaccagtttc accataaagg taacggcatt cgacatatca ttgaccaggt ttttggtggt
     9121 ctcgttaccg tgacgcagtg cgaagccgag gccgatcgcc cacaccagaa tcccgatgta
     9181 gttacctttc agcagggcgt cgatggggtt ggaaaccatg ctcattacca gtccgcgcat
     9241 cacttcgaca atgcctgacg gcggcgaaat atcacccgcg ctactggaca agtgcagggt
     9301 agaagggaag gcaaagctga agactactgc ggccagagct gcagagaagg tgcccagcag
     9361 atagaggaac aaaataggac ggatattggt tttctgcccg tgctggtggt tagcaataga
     9421 tgccattacc agcatcaaca ccaggatggg ggcaacagct ttcagtgcgc cgacgaacaa
     9481 agtacctaac agaccaacag cttccgccgc gggttttgag atccatgcca gaagaatccc
     9541 cagaacaagg ccgaccagga tttgttttac caggctgcca tgagccagac gccggaatag
     9601 ccccggtgaa cgttgcgtag tcatttttcg atcctttcat tgtgttgtcg cacatccctg
     9661 gtgcgttctg tcgacgcatc tttcaggatg taacaaagtg tttgcaaggt cgagtataag
     9721 gaaagttaca gcttcgggaa gcgaaaaatg ctggatttta aagagaggtc atgttttgca
     9781 acttaatggt tgcatttatt taacataaac agagggaagg actggctact aaggctgatt
     9841 agataacata tgggcaaaac tatttggaat atgagaacgg ttgcgctcct ttaacgtgtt
     9901 atgccatata aagtcactgg cgcagggagc gcgcaggggg cggccaatcg ccgccgcccc
     9961 ctgcaccccc aggctctggc gaacaaaatc gccgctgcgc ggttccctca gcctatccct
    10021 tgcggctacc gggtcgggcg caaggtaacg tccctgtaag atgcgccctc gccccggcct
    10081 ccagggaacg cttcggcgat tttgacgcca ccaacacccg tgcggtcttt ttgtaaagag
    10141 ctataagccg attacataat taatagctca cctttgtctg gaatgagtta tctctaggaa
    10201 tggcttaata tacagcgaat cattctcccc gctgcttatc atgccgataa ttcacccagg
    10261 cgttgataat aaacgtcatc accagaatgc caaacaccac gcccagcgag acggcgattg
    10321 gaatatggta gaagtcgaca atcagcatct tgataccgat aaacaccaga atcaccgcca
    10381 ggccatattt gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa tacatcgcac
    10441 gcaggccgag gattgcaaac aggtttgagg tcagcacaat gaacgggtca gtggtcacgg
    10501 cgaagatagc cggaatgcta tccacggcaa aaatcacgtc gctcaattcc accagaatca
    10561 gtaccagcat cagcggtgtg gcgtacagca atccattctt acgcacaaag aaatgctcgt
    10621 tgtcgatggt gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc ggcttgtcgc
    10681 caatgcctga ttcatcttca tgggcgagcg ccatcttcac gccggtaaac agcaggaagg
    10741 caccgaagat atacaggatc cagtcgaact gcgaaatcag ccagctgcct gtgaagatca
    10801 tgatggtacg cagaacaatc gccccgagca cgccatacac cagcacgcga cgttgtaatg
    10861 ccgccggaac ggagaaatag ctgaacaaca tcagccagac aaagacgtta tcgaccgcca
    10921 gcgatttctc aatcagataa cccgtgagaa aggccagggc ctgtggatcg gcaacggcgc
    10981 gaccttcggt ttgcaccaga taccaccaga aagcggcgtt aaacagtaac gacagcgtca
    11041 cccagaccag cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc ccacgacgcc
    11101 cctgcaacaa caggtcgata gccagcataa tggcgacaac aacagcgaat ccgccccata
    11161 gcaacggcgt gccgacagta ttcatagaag ttccttacac ataaaaaaac ggccaacgtc
    11221 ggaagacgtc agccgctgct ttttatgcat agacctcgcc ttccggcaag gtctcactta
    11281 caacaaaaaa ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc ggatgtggtt
    11341 tttcactcat cgtaatgacg atcgaccggc aatgaagtta ctcccctttg caggtaacaa
    11401 agtatgacag accattcagt cagtcaatgg tctgttacat accttttaca ctgttttact
    11461 ttggcaagta tagttttacg ctatctgccg gaaaaatcac cccagtttgg cggcggatct
    11521 cggtcagcag tttggctgtg atgcgactga ccgccagccc tggatgatcc accagatgct
    11581 catccaccag ggtagcgaac agctctgctt cgtagagcat ggtattaata tgctgcggct
    11641 gggtgagatc ctgcatctgg ctgccacgcg gcacaaagca gactttctgg cattcagaca
    11701 gtttttcaat caccagcgat cccgcttcgc cctgaatctc gcttcccagg acagaatcac
    11761 tgacctttga gtgctgcaag gtgacgctga aatcaccgta atccatcacc accacgccgt
    11821 gggcgtcaac gccgcttgcc agcaaactcg ccgttgcctg cacgcttttc ggctcaccaa
    11881 ataacgccac cgccgacgcc aggcagtaaa agccgatatc cataattgaa ccgttagaga
    11941 atgccggatt aaaggtgttg ggattctcac cgtccaggta acgttgatac cgcgaggaat
    12001 actggcaata gttgaaaaag actttacgca gtttgcctgc tttcggcagc gcctggcgca
    12061 acaaatgaaa gttcggcagg caggcggttt taaatgcctc aaacagcacc acctgatttt
    12121 cgcgcgcaca ggcaatggcg gcatccactt ccgccagatt cgacgccagc ggtttttcgc
    12181 aaatcacatg aattttatgg ctaaggaaaa gttgtgtctg ggaaaagtgc agggaattcg
    12241 ggctggcaat atacaccgcg tcaatggcat cgctttccgc catcgcttcc agcgacgtaa
    12301 acagatgctc gacagaaaaa tcattggcga agtgctgagc ctgctcaagg ctgcgggaat
    12361 atacggcggt taacttgtat ttaccgctct catgggcggc ctcgacgaac tggcgagtga
    12421 tccagttcgt accaatcaca gcgaaacgta tcataacgcg tggatactcc ataaagggat
    12481 tctgtcgtca atgtagcacg cctttttcat cttcgacgtg cgcagatacg cattattctt
    12541 tcgccggaca gccatgctca ctaaacggcc caatcatgac cgatggcccg agatatttcg
    12601 gctggcgatg gaggattttt gcgtcaatca ccgcgctgac acgagcgagt ttctcccgca
    12661 gttgagtata aaaaccgtgc ttcatattga ggtctggcgc gttaaacccg atagcgtcga
    12721 tgccgtactg ttttgccagc cagatggtgc gttggttatg aaattcttgc gagataatgg
    12781 tgatatggtt ttccccaaac acctttttgg cacgcactac cgaatccagc gttgagaatc
    12841 cggcatagtc gcaaaagatc actttcgccg gtacgccttt agcgatcaaa gcctgctgca
    12901 tacctgatgc ttcatcgtaa tttttacgtc cgttatcgcc actcaccagt agccatttca
    12961 cttttccggc gtggtacaac gctgctgcgg tatcaatacg tcgggtaaag tagcgattac
    13021 ccggcctcgc ccccagcaat aaacccacgt tacgcgccgg gacggcgttg acatcgcccc
    13081 aggtcagctg cttgctggcg ttgaccataa cgcgatcggc gataaaaatg gtcgctcctg
    13141 ccacaagtaa cagtaagcag gcaatcttta gagtccggcg agaaaaacaa atacggagaa
    13201 gaaggcgggc aaatgcgcgc agcatggtcg ttatccttaa cgaaaggggg gaggtcgatt
    13261 ttaggcgcta aagaaagaaa agataacgcc tgacatcatt ttcagatttt gtcctgccca
    13321 agcgacaact gcgttagcca cagccgggtg tcgaactcaa gctggtggta ctgcggctcc
    13381 atatggcagc acaattgata gaacgctttg ttatgctctt tctccttcag gtgcgccagc
    13441 tcgtgcacca cgatcatgcg caaaaacggt tccggcgcgt tgcgaaacac ggtagcgacg
    13501 cggatctctg cttttgcttt cagcttgctg ccctgcacac gagaaacagc ggtatgtaac
    13561 ccgagcgcgt tttttagcac atggatctta ttgtcataca tcactttatt gatcggcggg
    13621 gcattacgca gaaactgatt tttcagatcc tgagtatatt gccagagagc tttatcggtg
    13681 gcgtagtcgt gtgttcccgg atagcgtttt gccagcacat cgcccagacg ctgttcgtta
    13741 atcagtgttc gcacctgcga aagcagctgc tcgggataac cctgaagata agtaagattg
    13801 ctcataaccg cccacgaaaa agagaaaaag ggtatactca cgcacccttt tcaggggcaa
    13861 cgtcgaaatt ttatcattca ggagggccga tgagccactt agacaacggt ttccgttcac
    13921 tgacactaca acgttttccg gcgacggatg acgttaaccc gctacaggcg tgggaagcgg
    13981 cagatgaata tttgctgcaa cagttggacg acacagaaat cctcggcccg gtgttgatcc
    14041 tgaatgatgc ctttggtgcg ttaagctgcg cgctggcgga acataagccg tacagcattg
    14101 gcgactcata catcagtgaa ctggcgacgc gcgagaattt acgcctaaac gggattgatg
    14161 agtcgagcgt gaagtttctc gacagcaccg ccgactaccc gcaacagccg ggtgtggtgc
    14221 tgatcaaagt gccgaaaaca ctggcattgc tggaacagca actgcgtgcg ctgcgcaaag
    14281 tggtcacgtc ggatacacgt attattgccg gtgccaaagc ccgtgacatt cacacttcca
    14341 cgctggaact gttcgaaaaa gtgctcggcc cgaccaccac cacgctggca tggaaaaaag
    14401 cgcgcctgat taattgcacg ttcaatgaac cgccgctggc cgatgcgccg cagaccgtta
    14461 gctggaagct ggaaggtact gactggacta tccataacca tgcgaatgtc ttctcccgca
    14521 ccgggcttga tatcggcgcg cgcttcttta tgcaacatct gccagagaat ctcgaagggg
    14581 agattgtcga tctcggttgc ggtaatggcg ttattggtct gacgctgctt gataaaaacc
    14641 cgcaggcgaa agtggtgttt gtcgatgaat cgccgatggc ggttgcttcc agccgtttga
    14701 acgttgaaac caacatgcca gaggcgttgg atcgcagcga gtttatgatc aacaacgcgc
    14761 tctccggcgt ggagcctttc cgctttaatg ctgtgctctg caaccctccg tttcaccaac
    14821 aacatgcgct gaccgataac gtcgcctggg agatgttcca ctacgcccgc cgctgcctga
    14881 aaatcaacgg cgagctgtat atcgttgcca accgccatct ggattacttc cataaactga
    14941 agaaaatttt cggcaactgc accactattg cgacgaataa taaatttgtg gtgctgaaag
    15001 cggtgaagct gggacgtcgt cggtaagatt gggagttacc tgccggatgc ggcgtaaacg
    15061 ccttatccgg cctacgttca gcacgttacc cccaggccgg ataagacgcg gcaagcgtcg
    15121 catccggcaa tgtctgctca gttctggaat cgcgcttcca taaaacaaaa aatccctgtc
    15181 accatcctct acattctctt gtttagcgtt tttctacgtt tattcttccg tcacacagat
    15241 aaattcatcc gttgccaatc tgtcaacgga tgttatcata ttgccaattt gtcaacggac
    15301 gtgatgaaga atgcacctga taactcaaaa agcattgaaa gatgctgcgg aaaaataccc
    15361 gcaacataaa acggagttgg tggctctggg gaacacgatt gctaagggat atttcaaaaa
    15421 acctgagtca ttaaaagcag tattcccatc tctggataac ttcaaatatc tggataagca
    15481 ttatgttttc aatgttgggg gcaatgaatt acgtgttgta gcaatggtct tttttgaatc
    15541 gcaaaagtgc tacatacgtg aagttatgac gcataaagaa tacgatttct ttaccgctgt
    15601 tcatcgtact aaggggaaaa aatgattgcg attgccgaca tcttgcaagc aggagaaaag
    15661 ctaactgctg tggcaccttt tctggcgggt attcagaacg aggaacaata cacccaggcg
    15721 ctggaactgg tagatcatct gctgctcaac gatcctgaaa accccttgct ggatctggtg
    15781 tgtgccaaaa taaccgcgtg ggaagaatca gcgcccgaat ttgcggaatt taatgccatg
    15841 gctcaagcca tgcctggcgg tatagccgtg attcgtaccc ttatggatca atatggttta
    15901 accctttccg atctgccgga aattggcagt aaatctatgg tgtcacgcgt tttgagcggg
    15961 aagaggaaat taacgctgga acacgctaaa aaattggcaa cgcgattcgg catttctccc
    16021 gccttgttta ttgattaacg cgacgtgccg aatgcgttgc atccggcgct tcaaataacg
    16081 atttaaatct ccagcgccag ccgtgttccc tgggcaattg cccgtcgtgc gtccagctcc
    16141 atagccacat cgcagccgcc aattaaatgc acagttttcc cgctatcaat cagcggttgc
    16201 gccagcgcgc ggtttggctc ttgccctgcg cagatcacca cattgtccac tgctaatacc
    16261 tgcgtttcgc cgttgatcac cacatgcagc ccgtcatcgt caatcttctg ataacttacg
    16321 cctgggatca ttttcacacc ccgcgagagc agggtggtgc gatggatcca gccggtggtt
    16381 ttgcctaacc cctgtcccgg tttgctggct ttgcgctgga gcatcacaat ttgccgtggg
    16441 ctacgcggga tctgcattcc ctgcgggctt aagccaccag cctgttgtag gctgctgtcg
    16501 atcccccact cattacagaa cctggcgata ttctggctgg tggattcgcc cggctgactt
    16561 aaatacatcg ccgtatcaaa accaatcccg ccacaaccga tgatggcaac cttgttgcct
    16621 accggcgctt tgtcgcgcag tacatcgaga taactcaata ccttcggatg atcgatcccg
    16681 tcgataggcg gaacacgcgg cacgatccca ctggcgagga tcgtttcatc gaacgcctgt
    16741 aactgatccg ccgtcacggt gtgattgagt ttcagcgtca cgcccgtcac ttcgatcatc
    16801 cggcggtagt agcgcagcgt ttcgtaaaac tcctctttgc cggggatctg tttggcgata
    16861 ttaaactgcc cgccgatctc gctgtgcgca tcaaacaatg ttacctgatg cccacgcgcc
    16921 gccgcgttaa tggcaaacgc cagcccagca ggtccagcgc cgaccaccgc cagatttttt
    16981 ttctgcacgg cgggaaggat tggcattttg gtttcgtggc aggcgcgagg attcaccagg
    17041 cacgaggtga ctttgccaac gaagatctga tcgaggcagc cctggttgca gccaatacaa
    17101 gtgttgatct catcggctcg ccctgattgc gcttttgaca gcagttccgc atccgcgaga
    17161 aacggtcgcg ccatcgatac tatatcggca tcgccgcgcg agagaatatc gtcggcaacc
    17221 tgcggatcgt taatccggtt ggtggttacc agcggcagcg agacgtggcc tttcagtttg
    17281 cgcgtgaccc agctaaatgc gccgcgcggc acgggcgtgg cgatggtcgg aatccgcgct
    17341 tcgtgccagc ctattccggt gttgataatg gtcgcgcccg ccgcttcaat ggcctgcgcc
    17401 agttctaccg tttcggcaaa agtgccgccg ccttcgacta ggtcgagcat cgacaaccgg
    17461 taaataataa tgaagtcgtt gccgacgcgt tcgcgcaccg tacgcactac ttctacggca
    17521 aatcgcatcc ggttgcggta atcgccgccc cactggtcac tacgctgatt ggtgcgcagc
    17581 gtcagaaatt cgttgatcaa atacccttca gagcccatca cttcgacgcc atcgtatccg
    17641 gcctcccgcg ccagttgcgc gcagcgggcg aaatcgtcga tcagttgcag gatctcttca
    17701 tgggtgagtt cgtgaggaac gaaacggttg atgggggcct gcaatgcgga cggtgcgacc
    17761 agatgcggtt ggtagctgta gcgcccggta tgcaaaattt gcagggcgat tttgccgcct
    17821 tcctgatgta cggcttcggt aatggtgcga tggtgtggga tctggctggc gtcgttgagc
    17881 attgcgccac cttccatgcc aacgcctgtt aaatctggtg caatgccgcc gctgacaatc
    17941 agcgccacgc cgtgacgggc gcgttcggca taaaacgcag ccagccgttc agcaccgtcc
    18001 gggtattcct ccagcccggt gtgcattgaa cccatcaaca cgcggttttt taacgtggta
    18061 aaacctaaat ccagcggggc gaacagcgac gggtagctca taaaattgtc cagtatgtaa
    18121 aataattgtt atgtagtcgg atgagttcta atttagccgt cgatggagaa aagggaaaag
    18181 gggagtgcgg ggtttgtgat gggattcaaa aagaatacgc ccggtggtaa cgccggatgc
    18241 gacgcttgcc gtgttttatc cggcttgggg gattgcacat gtagggcgga taaggcgttc
    18301 acgccgcatc cgccagccgt tgcaaattac tgcttacgga aaaaatcgtt atacaacata
    18361 tacaaatgag cggcactcca ggagaaattt ggtgcgcctt gctgcgcgcc tgtcagcggg
    18421 ttgtaattct cctgaattgg gccatcggcg gttaatcctt tggcgtgcct gaagaacgta
    18481 tccgccagct tcagggcatc atcacgataa ccgtaacgct ccatcccttt cagaccaaac
    18541 cagaactgat ccacccatac gcgcccgcgc cagtagatat cagcgccaaa agccggattg
    18601 gttaacgccg ccgttcccag cggaacaaat gtgttgaact ctttaggatc gagcatcacc
    18661 ttcaccaccg cgtcggcatt ggcctgcgtt gccgcaccgt taaacagcgg cgaccagcct
    18721 tccggccctt taccgcgctc aacaatcggt ttgcccgcgc agccgttcgc cagtggttta
    18781 tcttcaatac gtacgtcata gtagaactgg gtagtcgggt cgaacataca ggtgttgatg
    18841 tagtccgcga gctgctgtgc caactggcga tagcgtttgg cctcttctgg cttaccgagg
    18901 atggttgcca tctccgccag ataatggtta tcgctgtaca tatagctggc ctgatccacc
    18961 gactcctgca atagcgagta gcccagcaac gttccgtcct gactgcggtt ttcggcgaat
    19021 ttcaccgtcc agtcgctacg tttgccgcca ttagcgacat atttatccaa ctgttctttg
    19081 tcgataaaac caaagacggc ggcatcatca cgccccgatt cccacgacgc agcgacctgt
    19141 gccggaattt ccagactgtc gtactggcct ttctccacca cgcgggcgta gttgttcagc
    19201 ccagactgcg tctcttcttt atcgcctttt ttcaccgtaa acagcatctc gccgctctcg
    19261 gtgttgtggg ctttgtcgcg ggtcgcgcca tattccggca ccccgttgcc gttatgatcg
    19321 cggttacgta accaccagtc gtgataggcc accagtttcg ggtacatctc tgccaaccag
    19381 gctttatcct gggtgacgtt gtatacttcc atcaccgacc aggcggcaag gctgggtttg
    19441 gtattacgtt cgttccagtt gccgccatcg ccaccacgct cggggctaag gttccacgct
    19501 atcaggtcgg ggacaaagcc cacatcctgc ggacgcacgc tgtcgccagg ctggatctgc
    19561 caggagaaga ccgcgcggat attctctttg gcgatatccg gattgaaatg cgccatcgca
    19621 aacgcctgct tccaggtatc ccacggccag gtctgattgc cggagaacca gcgtccggtc
    19681 accgacggcg tgacggtgtt ataattcacc gcaccgcccg gtgagcgcca gttaccgttg
    19741 agcgtttcga tcgctttcac cgcgacgcgc gtctgttccg gcgtcgcatc cggattggtt
    19801 aaccctttct tcaggtattc ttcccagcgt tgctgcgagg cggtgagata aaacgccgga
    19861 cgcgccagaa tatcgcggat ctgcatttgc tctttgctaa cttcctgagc ggtcagcaga
    19921 tgggaatagg tggtgtagag cgtggtcgaa ccgttgatat gcgccttact ggtaaagcga
    19981 ttaccgttga tttcagtctg caccggcagg gatttatgca cctgatattc cgattcgccg
    20041 gaggtcagca gatcccaggt ggcgcgcact ttgccaaagg tgactttcag gccatcacgg
    20101 gtggcgctga ttttgcgctg atagtcaggg tattcgccag caatggtttt atcggaaagc
    20161 ggtttccctt ctttcgcttc cagtttttcc agcagttcgc catcccacac cagatccagc
    20221 ggtttattgc tgatgatttt agtttccagt agtgacgtgc gcggcgtggc gaagcgcaga
    20281 gtcatttcga cctgcacatc ttttgctgtc agtttttgca ccagcgcgcc gggaatactg
    20341 tatgcctcca gcgtgaagtt gactttcttg ccgtcttgcc agacagtcag gcggtcgaaa
    20401 ttgctggcca taaagttgat gtattcttcc gtcagcagcg caacgcccgg aaagccgccc
    20461 atggtgttcg ggccgtctgg caacagatga ccatgccagg ctccgagatc gaaaaacgga
    20521 ttaaaacgct ggtgatcgtc gtaatcgtaa tccttcatgt actgcggcgc accagtacgg
    20581 ttaatcacgt ttttataatt gtcggcgtta gcggcatgag cggaaaaact tatcagcaga
    20641 gcgcaggtta ctggcgttaa aatagttttg attttcatga gattacagtc cttattacca
    20701 gtagaaatat tgcatcagga agatttggtc gctgggatcg ttagaggcat ttttcatgta
    20761 gaagcggcta tcaaaagccg tggcgaaacg tccgccgcca tattcgtacc agataccaaa
    20821 ctgcatgaaa gaggtggttt catcgtcagc gtggtcaggg cgatgtttgg cgtaggtgta
    20881 agcggtggag agataaacgc cttttaacgc ttcatacatg gcgctcacca tcatcccgct
    20941 ttcacttccc ggtacgtcca gcccgtcggc acgtccggta tggtgccagg cgcgagcggc
    21001 aaagttaggg gtaaacaggc ccgtgagata gagttgtcct gtgccgtcgg taatttccag
    21061 accattcatc caggtgatgc cttcggcaag gtcatactgg atgtaaccgt tcatccattg
    21121 ccggataggt gtatttgtcg tcgtagcgat cgtacttgcc aaaatgtagc caggctttgc
    21181 tttcgtcatg atgtccggca ggtgtggcgg tgacgctata acgaaagttg ccgcttaagt
    21241 tctgtacctt cagcgcggtc ataatgtcgc gggtattggg aatgacgtag cccaaatcgg
    21301 gtgtgaagtc gccccaccat tgcaggtcat ccagcgagga atcctgacgc agactgagca
    21361 tgatttcggt gccatcattg gtacgataac cgccatagaa acggttaatc ccaccttcga
    21421 aaccacccca gctatggtca ggcacctagg cattaccgtc gtggtcggcc tgcacggtcc
    21481 agccttcgcc gtaaatcagg ccgaaccagg agccgtactt catctccagg ccaccttcaa
    21541 tataggtgcc gtcgctgtat ttatgtttat tctcaccctc taaatccata tatccaccaa
    21601 gccctaattc gccgtaaaaa cgaaatgaag gagaagatgt cgaggtaatt gcgggtgttt
    21661 ttgtgtcgtc atgttctgcc ggacgtgctt cttctcctgc cgcagaaaag gcgtagcaaa
    21721 acggcagagc aagtaatgct ctgcatggtg cagtgattaa tttcattgtg ttaatccatg
    21781 agctaaagaa aaagtacagc cttttatcgt tgtaattata aaaatataaa ggcagaaact
    21841 attttccctc tcctcatcca ggaagaggga aaatgcctgc tcatttttta ttagtctgct
    21901 taatcaacat tgctggctgg cgtcgcttca atatggcgct cttcagccgt taatcccttt
    21961 atatatttgc tgtatttcca ccacgcaaag ccgaggaaga taacaatacc gccgacgtta
    22021 taaaaaatga tggtcagaat attcgcgcca gtcgggaatg tcgaagcgac aaaccctacg
    22081 gcaaatatcg caatcagcat tgaaacaacg ataataccgg tgcggcggga gcccatacgg
    22141 aaatcgcgtg gcaggtgatc taatttggcg cgtaaattca ggtaagccag catgatgaat
    22201 aacggcggaa gcatggacgc tgcggcggtc atattaataa tggtattcat cagatcctgc
    22261 acggtattgg aaccgagcat cgggataatc atcagcggga tgacgatcag aaactggatc
    22321 catgctgcgc gcgccggaac gccgttttcg ttaagttcga cggttttctt accaaagatg
    22381 ccttccggga tttcggagaa gaaaattttc accggcgttg cggtccacat cagcagggaa
    22441 ccgaacattg cggtaaagga caccagaccg acaaagcgat tcatcaacgc ttccggcaga
    22501 ccaaaatacg ccgccatgcc gtggaatacc tgcaccgatc cgccggtaaa tttcaactct
    22561 ttgctgctga cgaagacgtt aatcagcacc gaggagacgg aatacagtac gccgataaaa
    22621 atcccggcga ggatgatcac tttaacgaac gatttcgaac cgcctttgac gtcgttaacg
    22681 tacaccgcga cggactccgc gccacctgcg gccataaaga tccaggtggt aacgccgagg
    22741 aacgcccagt tgaagttcgg gatcatcgcg tcaacggtga tcggatcggc aggctgtacg
    22801 ccgccaacca gtgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc
    22861 gttgaagtga cggaggtaat tggccccagc attttcgccc cgttggtgga aacccaggtg
    22921 gagaaggcga acagcaccat actgataacg gtggtggcaa ccggcgtcat aatatattcg
    22981 tagccgagga aggcatacga agcataagca ataacgcgcg gcaacagcga ggtgaaaaag
    23041 aacagattta cgaaccagta agtgtaggca gtaataaatg cccaacgtcc gcccagcgaa
    23101 ctttttaccc acgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg
    23161 atgatcagac agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag
    23221 gccagtccaa gctcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa
    23281 gtcagcgaga gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat
    23341 ctccgaaaat gtgtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt
    23401 tgttatggaa ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat
    23461 tgcagataaa ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct
    23521 ctacggtttc tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca
    23581 ctacctgtaa tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat
    23641 gcacttcaaa atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tcattttcca
    23701 cacggtaggt caatgaatca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat
    23761 tttcaatcgc ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact
    23821 gttctaagtt atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa
    23881 ccctgcgccg aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg
    23941 cagcaacgta aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc
    24001 gctgccccag gagttggagc cgaggccaag tagctggtgg tcgaggttca gggtgatgtc
    24061 atcactgcgc tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct gggtatagtg
    24121 ccaggcgctg aagttaattg ggcgctgcgg aaccaccagc agaccgttgc cgtggcggtt
    24181 agtcagtgcc gtccagcgga catgctgacg gttgccgttg ttctgcggga agggatagtt
    24241 ctcgaacatg gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc
    24301 ggcgtagttt tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat
    24361 ccccatggtg aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga
    24421 aagcgccacg ttaacctggc catcggcagc gatgcgccag atgtaggtac agcgcatccc
    24481 gaagtcaaac accggcggtg caataactgt gcggctgatg atcaacactt caccatcgct
    24541 ctgttctacg gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca
    24601 caacccttcg tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg
    24661 ctcgcgggtc agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt
    24721 tgagaaggtg atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag
    24781 cgtcagcgga cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag
    24841 cgggaactga taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt
    24901 ggtcaccgta atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa
    24961 gggggcttcg ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc
    25021 accttcggcg cgcacctctg cgtgcagggt gtagtcatca agcgtggtaa accacagttt
    25081 attttcgact ttcaactcgc cgcgagtcag cgcgtggatt tttaccggcg cgataacctg
    25141 tttgtactct ttcaggcccg atcccggcgt ctgatcggaa tagatcaaac catcaagaca
    25201 gaagttatag ttgttgggat agtcgccgta gtcgccgccg aatttatacc agacattgcc
    25261 gtggtcgtcc tgtgcctgga tcccgtggtc gcaccactcc cagacataat gaccctgaat
    25321 gcaatcgtgc ttatagaaga cgttctggta ctccgtcagc ccgcccggtc cgttccccat
    25381 cgcatgagca tattcacaga tgatgcgcgg cttcggatgc gggtattcac caaactcatt
    25441 catcagcggc acgcgggtgt acatggtgga aataatatcg accacttcag catcgcgatc
    25501 ttcttcgtaa tgaaccagtc gcgtgtcatc cagcgctttc gccgcatggt acatcgcgcg
    25561 gatgttacag ccatagccgg attcattgcc cagcgaccag atgatgatcg acggatggtt
    25621 tttctgtgcg tggatatggc gaacaatgcg ctcgacgtag actttttccc actgcggatc
    25681 gtcggtaata cggctgatat cgccgacatt agcaaagccg tgcgattcga cgtcggtttc
    25741 cgccatcaca aacaggccgt agatatcaca cagttcgtaa aaacgcggat cgttcgggta
    25801 gtgagcggta cgcacggagt tgatattgtg ctgcttcatc aactggagat ctttctcgac
    25861 gcgatccatt ccaacggcgc ggcctttgcg atgatcgttg tcgtgacggt tgacgccgtg
    25921 cagcatcaca taacggttat tgatccagaa cagaccgtcg cgcactttga tatcacggaa
    25981 gccaacgcgt tgtggcacca cttccagaac gttgccgttg gcgtctttca gcgtcatgac
    26041 cagatggtaa agataagggg attctgctga ccattgctgc ggctgttcga cagtaaaagc
    26101 aaagctggcg ctggtcagtt tttcaattgc caaatgatca atggcgctgc tgtgcaccac
    26161 gcgttcgcca tcaaacaggg tatattccag cgtcgtgacg acaggggagg cggcgagatt
    26221 ttccagcacc acttcgcagg aaagcgtggc atcgcaatag gcttcgtcaa agtcggtacg
    26281 cacagtgaaa tcgttaatat gcgttaggtg ttttccgacc agataaacat cgcggaagat
    26341 ccccgccgac caccacatat cctggtcttc cacgtaggta gagtccgccc actgcatcac
    26401 gcgcacacac aacaggttgt cgccggtttt aaccatcgcg ctgatgtcaa actctgcggt
    26461 caggcgactg cccttgctga aacccacata ctgaccgtta acgtagactt caaaataggt
    26521 ttcgacgccg tcaaatttaa tcagcgtctg tttaccctgc cagccgtcgc tgagggtgaa
    26581 aatacgttga taggcaccgg ttgggttatc gctggggaca aacggcacat cgatggggaa
    26641 cggaaaacct tcgtcggtat attgcagttt gccgtgacct tccatttgcc acatggcggg
    26701 gacggtaata tgcccccagt cagccattaa ctcagaggtg aaggcttctg gtacttgcag
    26761 cggatggtca aaaaagtgga aattccactg actgcttaag ggcagaaaca ggctgctggt
    26821 ttcgcgggca aaggtacgcg cttgcgcaac agaatcatat gaaaaaaagt acgcacgcgg
    26881 cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag cgattcatag ggcctcccgg
    26941 tagagagaaa tcggaaccat tcatggtgat ggtagtttgt gtaattgctg ggggtttagt
    27001 aaaattttta ctaaaaagca gcaggtaaat gcatttactt taactggatc acgaaaataa
    27061 acggcagcac tgtccggcaa aaatgattgc cgaacagtgt gtaaggggat ttaacgggtc
    27121 gtgccgcgca gttttaattt gctgggaacg aagactagca gcggcagcgc gcgaccatcg
    27181 cgggcttttt catacaccag gttaacgccc tgacttccca tcatctcgga atggatgcgc
    27241 acggtggaga gcggcggaaa ggtaaatcgc gcggtgggga tatcgttaac gctgataagc
    27301 gaaatatctt gtgggatgtt caggccacgt tcatgaattg cccgcagtac gccgatagca
    27361 atggaatcgg aagcaacaaa cagtgccttc ggatagtctt cccgcgccag catttgtttt
    27421 gccagttcat aacccgacga actggaaaaa ccgccgcgcc agatatcctc ttcgcgtacc
    27481 acttgtttca gtcggccata ttccgcaaag gcgacctcac gaatatccgc cttgccaggc
    27541 tcatcttcac cgccaataaa accaatacga ttaacgccct ggttgatata gaagtcgatg
    27601 atttctttac tgatgcgtgc cagatcgata tccaccgcat cgtaaccgct gccgggttcg
    27661 tgaaagtcga taaaacagat attgtcggtc aacgcgctgg cagcggcgcg cagggcgggc
    27721 gtgggtttgc cgacaattaa aataccggtg acgtttttaa tgtctggtaa tccgttgtgt
    27781 tcataacagt tggtcagttc gatcgccagc ttttcgcact gagtttcaat gccgtggcgg
    27841 atcgccagat agtaaggatc gttgatctcc agctcctgct ggtagctgta gatagccaga
    27901 atatggtgtt ggttgactgc gcctgtctgt agtttacggg cgctactggt cttgtactcc
    27961 agcttttcgg cgatctcgag aatgcgatgt ttcgtctctt ctttcacatt caatgtcgga
    28021 tcgtcattta agaccctgga tactgtcgcc agggatacgc cagcttcgat tgcgatgtct
    28081 tttaatgttg ccatgtttac cttttttact ttccctgatg tgcgccccca ttgtagagga
    28141 agtgcttatc gggtcacctg ttttcagtaa ataaagcgcg tttctttgac gctgtaacgg
    28201 taacacagcg gcttaggaat gtgatcacgc ctgcattctc catttttagc ctacaggatt
    28261 gagaaaaact tttctatagt ttagtaaaat tttactcgaa gggaggaagc aatggaaacc
    28321 gtggcttacg ctgattttgc acgtctggaa atgcgcgtcg gaaagattgt ggaagtgaaa
    28381 cgccatgaaa acgccgacaa gctgtacatc gtacaggttg atgtggggaa aaaacactgc
    28441 aaaccgtgac cagcctggtg ccgtactaca gcgaagagga gctgatgggg aaaacggtgg
    28501 tggtcttatg caatctgcaa aaagcgaaaa tgcgtggtga aacgtcggaa tgcatgctgt
    28561 tgtgtgcgga aaccgatgac ggcagtgaaa gcgtgttgtt aacgccggag cggatgatgc
    28621 ctgcgggcgt acgcgtcgta taaaaagatc ggatggcgac gtcgtatcgc catccgattt
    28681 gatattacgc ttcttcgaca cttactcgca tggccgccag cgccttacgc gccgctttga
    28741 tcaccagttc acactgttca atggtcagtg tcagtggcgg ttcaatgcgg atcgttttgg
    28801 cgttattgag cgttccggcc accagtacgc gctggcggaa catctcgctg gcaaagttat
    28861 agccgatttc gttatcaaca aactcaatcg ccatcaacat ccctttacca cgcgcttcct
    28921 gtaccagatc gggatattcc cgcgccagtt gacggaaacc gtccagcaac atatcgcctt
    28981 tttgctcagc ctgagccggt aagttctgct ccagcaacac attgatggtc gccagcgccg
    29041 ccgcacaggc cagcgggttg ccgccaaagg tggtggtatg caggaatggg ttgtcgaaca
    29101 gaactgaaaa cacctcttca gtggcgatgg tcgcgccaat cggcatcacg ccgccgccga
    29161 gggcttttgc cagacacaga atgtccggct gaacgttctc atgttcacag gcaaacatct
    29221 tgcccgtgcg ccccatgccc gtttgtactt catcgaggat catcagtgcg ccgaactcat
    29281 cgcacagctt acgcacggcg gtgagatagc ccggcggcgg cagaattacg ccaccttcgc
    29341 cctgaatcgg ttcgaggatc accgcagcca catcatcacc ggtttttttg cactcattaa
    29401 gagccgtgcg catggcttcg atattgccaa acggcacatg acggaaccct ggcagcagtg
    29461 gcataaacgg tttgcggaag gtcgatttcg ccgtggccga cagcgcgcca agtgatttac
    29521 cgtggaacac gccgctggtg gcaataaaag taaatttgcc gcgcggcgac tgataagcct
    29581 tcgccagctt cagcgccgct tcaacggact cggtgccgct attacagaag aagctgtatt
    29641 tcagtttacc gggcgttagc gcagcaaggg ttttcgccag catagcccgt aacggatcga
    29701 gtagctcctg gctgtgcagt ggttgtttcg caagttgatt ctgtacggcg gaaaccacaa
    29761 ctggattacg gtgccccacg ttgaaaattc caaaacctcc caggcagtcg ataaactcct
    29821 gtccctgggt gtcgacaagc gtatttaaac ctcccgcttg ccactctacg gctccgtaat
    29881 ccccgccggc ggtaacagat ttgcgatact ctaaaaaccc cggattgaca tgctctttga
    29941 agtattcaat cacctctcgg ttaagtgctt tcatctcctc atgatccagc gttcgcttct
    30001 caatgagatt cagggcgtgg gcgctgcacg ctaaagccga tgcgctcgaa ggtaacctgt
    30061 tcaaaatatg ctccggaggc tcgcgtatca catgataccg atttaagtat tgcagggatt
    30121 gcgccactcc ggctgcgatc gcgaaaatcg ggataaacac aaatgcggtt atcatttgtg
    30181 caatatttaa tgctatccgt taacatttgt tgttattttg cacggttttt gtcgggtatg
    30241 gatttgatga ttgctgcgca aatgcacgtt ttcagggcaa taattgcctg atttttgggc
    30301 aagataatta acgttttcgt atagtaacag agtgaaagta ttttaaatca aagaattaga
    30361 tttgtgcttt aatcgcaaat tgcgatctaa atcaaattaa tcggttaaag ataaccgcag
    30421 cggggccgac ataaactctg acaagaagtt aacaaccata taacctgcac aggacgcgaa
    30481 catgtcttct catccgtatg tcacccagca aaataccccg ctggcggacg ataccactct
    30541 gatgtccact accgatctgc aaagctatat cactcatgct aatgacactt ttgtgcaggt
    30601 gagcggcttt accttgcaag agttacaagg gcagccgcac aacatggtgc gtcacccgga
    30661 tatgccaaaa gcggcgtttg cggatatgtg gttcaccctg aaaaaagggg agccctggag
    30721 cggcatcgtg aaaaatcgcc gcaaaaatgg cgaccattat tgggtgcggg ccaatgcggt
    30781 accgatggtg cgcgagggaa aaatcagtgg ctatatgtcg attcgtaccc gggcgacgga
    30841 tgaagagatt gcggcggtgg agccgctgta caaagcgttg aactccggac gtaccagtaa
    30901 gcgtattcat aaaggcctgg tggtgcgtaa aggctggctg ggtaaactgc cttcattacc
    30961 gcttcgctgg cggacgcgtg gagtgatgac cctgatgttt atcttgctgg cggccatgct
    31021 ttggtttgtt gctgccccgg tggtgacgta tttcctctgt gtgttagtgg tattgttggc
    31081 aagcgcttgt tttgaatggc agattgtgcg cccgatagaa aatgtcgccc gtcaggcact
    31141 gaaggtggcg accggagagc gtaatagtgt tgagcacctg aatcgcagcg atgagctggg
    31201 gctgacatta cgcgcggtag ggcagcttgg cctgatgtgc cgttggctaa ttaacgatgt
    31261 ctcaagccag gtgtccagtg tcagaaatgg cagtgagacg ctggcgaaag gcaccgatga
    31321 actgaacgaa catacccagc agacagttga taacgttcag caaacggtgg cgaccatgaa
    31381 ccaaatggcg gcgtcggtga aacagaactc tgccacggcg tcggctgccg ataaactttc
    31441 tatcaccgcc agtaatgcgg cagtgcaggg tggggaggcg atgaccacgg tgatcaagac
    31501 aatggacgat atcgccgaca gtacctagcg cattggcacc attacttcgc tgattaacga
    31561 tattgcgttt cagaccaata ttctggccct gaatgcggcg gtggaagcgg cgcgtgccgg
    31621 cgaacagggc aaaggttttg cagtggtggc aggggaagtg cgtcatttag ccagccgcag
    31681 cgctaatgct gccaacgata ttcgcaagct gattgatgcc agtgctgata aggtgcaatc
    31741 cggttcgcag caggtacacg ccgccggacg gacgatggaa gatattgtgg cacaggtgaa
    31801 aaacgtcacc cagttgatcg cccagattag ccattcaacg ctggaacagg ccgatgggct
    31861 ttccagcctg acccgtgcag tggatgagct taacctgatc acccagaaaa atgccgagct
    31921 ggtggaagag agtgcgcagg tgtcggcgat ggtgaaacac cgcgccagcc gactggaaga
    31981 cgcggtgacg gtgctgcatt aatcggtatg ccggatccgg cggttggagc acaatgcctg
    32041 atgcgatgct ggcgcatctt atcaggcctg cggggtgtgt agcgggtgta ggccggataa
    32101 ggcgcgtcaa gcgtcgcatc cggcaattgc accgcgccac tggcggatgc ggcgattaat
    32161 ccagttgcgt aatatcaaaa gcggcgcggt cgatcaccgc aatgatcttt tttatttgtg
    32221 catcactgat atcgctctgg ttgacgcgta aatccagcac cgctttaaaa ttatccagcg
    32281 cccgcttcat ttgcgggttc tggcgcagcg ccgcgccaac gcaacgcgct ttgatgcgtt
    32341 cttcaatcat ctccacttgt tcgcggtttt cttccagcca ctgcgcgcct tgttcggtca
    32401 gcgcaatctg cttcttacca ccttcctctt cgcggatggt aatcagcgac tgctcctgca
    32461 gaaaatccag cgtcgggtag atgacgcccg ggcttggggt gtaattcccc tgggttagat
    32521 tctcaatcgc tttaatcaat tcgtaaccgt ggctgtcatc gcgcgagaga atatccagaa
    32581 tcaccagacg caattcaccg tgaccaaaga aacgttgccg acgaccgccg ccgcgaccgt
    32641 ggcgaccacc gcaacattga ccgtgttcat gctggtgacc gtgtccgcag tgctcgtgtt
    32701 ctgacttctc acctttgcag cagccctcat ggcgtggctg gccttcatgt ttacaacacc
    32761 cttcgtgatg atggctcata tcagcctcct tttgcttgat tagatatatc taatttatat
    32821 ctgatgtgag cattactgca agtacaaaaa tgatttttaa acgtggaagc acctcacatc
    32881 taggccggat aagacgttca cgccgcatcc ggcagcattg ctatggcact gaatgccttc
    32941 acatacttca gacatatcag ttctacaaat cgcttgcatt tatcatgatt aacaatcatt
    33001 atcatttgcg aattttattt agatatatct gattaccatc acgaaggcga taacaatgaa
    33061 taacaccccc cgctacccgc agcgcgttcg caatgatctg cgcttccgtg aactgactgt
    33121 gctacgcgtt gagcgcatca gcgccggttt tcagcgcatt gtccttggcg gcgaggcgtt
    33181 ggacggtttt acatcgcgcg gctttgacga tcacagcaaa ctcttctttc ctcaacctga
    33241 cgctcacttt gtgccgccaa cggtaacgga agagagcatc gtctggccgg aaggaccacg
    33301 cccaccgtcg cgtgactata cgccactgta tgacgaacta cgccatgaac tggcgatcga
    33361 tttctttatt cacgacggtg gggtcgccag cggctgggcg ataaacttac ggtggcaggt
    33421 ccgcgcggtt cgctggtggt gccggaagat tacgcgtatc agctgtatgt ctgcgatgaa
    33481 tccggaatgc ccgcactgcg ccgccgcctg gaaacgttga gcaaacttgc cgttaaaccg
    33541 caagttagtg cgctggttag cgtgcgggat aacgcctgtc aggattatct cgcgcacctt
    33601 gatggtttta atatcgaatg gctggcacat gatgagcagg cggtagatgc gcgtctggcg
    33661 cagatgcaaa tccctgccga tgattacttc atctggataa ccggcgaagg taaagtcgtt
    33721 aagaatttaa gccgccgctt tgaagcggaa cagtatgacc cacagcgggt acgtgcagcg
    33781 gcttactggc acgcaaagta actgatatat aaaaagaaaa aggctgacga tttctcgtca
    33841 gcctttgcta tat
//
LOCUS       DAJTYH010000038        32405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTYH010000038 DAJTYH010000000
VERSION     DAJTYH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(2..94)
                     /locus_tag="OO609_001766"
     tRNA            complement(2..94)
                     /locus_tag="OO609_001766"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO609_001767"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO609_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6633217.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO609_001768"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO609_001768"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6633218.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO609_001769"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO609_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6633219.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO609_001770"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO609_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6633220.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO609_001771"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO609_001771"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6633221.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO609_001772"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO609_001772"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6633222.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO609_001773"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO609_001773"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO609_001774"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO609_001774"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6633223.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO609_001775"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO609_001775"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6633224.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO609_001776"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO609_001776"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6633225.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO609_001777"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO609_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6633226.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO609_001778"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO609_001778"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6633227.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO609_001779"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO609_001779"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6633228.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO609_001780"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO609_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6633229.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO609_001781"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO609_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6633230.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDTLELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO609_001782"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO609_001782"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6633231.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO609_001783"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO609_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6633232.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO609_001784"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO609_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6633233.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO609_001785"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO609_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6633234.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO609_001786"
     CDS             complement(20900..21019)
                     /locus_tag="OO609_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6633235.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO609_001787"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO609_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6633236.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO609_001788"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO609_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6633237.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OO609_001789"
     CDS             complement(23682..25499)
                     /gene="hycC"
                     /locus_tag="OO609_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6633238.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKS
                     VLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIY
                     QSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATC
                     APLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACP
                     LLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKW
                     LNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OO609_001790"
     CDS             complement(25536..26147)
                     /gene="hycB"
                     /locus_tag="OO609_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6633239.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OO609_001791"
     CDS             complement(26272..26733)
                     /gene="hycA"
                     /locus_tag="OO609_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6633240.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26945..27295
                     /gene="hypA"
                     /locus_tag="OO609_001792"
     CDS             26945..27295
                     /gene="hypA"
                     /locus_tag="OO609_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6633241.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27299..28171
                     /gene="hypB"
                     /locus_tag="OO609_001793"
     CDS             27299..28171
                     /gene="hypB"
                     /locus_tag="OO609_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6633242.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28162..28434
                     /gene="hypC"
                     /locus_tag="OO609_001794"
     CDS             28162..28434
                     /gene="hypC"
                     /locus_tag="OO609_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6633243.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28434..29555
                     /gene="hypD"
                     /locus_tag="OO609_001795"
     CDS             28434..29555
                     /gene="hypD"
                     /locus_tag="OO609_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6633244.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29552..30562
                     /gene="hypE"
                     /locus_tag="OO609_001796"
     CDS             29552..30562
                     /gene="hypE"
                     /locus_tag="OO609_001796"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6633245.1"
                     /translation="MNNIQLAHGSGSQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30636..>32405
                     /gene="flhA"
                     /locus_tag="OO609_001797"
     CDS             30636..>32405
                     /gene="flhA"
                     /locus_tag="OO609_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6633246.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLS"
BASE COUNT         7639 a         9045 c         8435 g         7286 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacact
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagcaggat aatgccgata
    24661 ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt gttccatcaa cgcatacaga
    24721 ccaccgacga acgcggtgat catgccgagc accagcagcg cgatccccca ccacagcggt
    24781 gcattaccgc ccagcagtga cagggttaac atgcccagca ggccaatttt catgactacc
    24841 gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg cacccagccg
    24901 tgcagcggaa taatcccagc cagcaggcca aagccaatca caccgagcag ccagatatcg
    24961 gaaccgagcg gcagctgttg catacgcata tccagcaggc gcagatccag cgtgccataa
    25021 cgctgccaca gcagccagca agcaatcacc agcagcagag tgccaagacg cctcagcgca
    25081 aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacaa ggccatgact
    25141 tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac ggcggcagcc
    25201 atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg ccagtcaatg
    25261 ttatagaggc tgacaaacag accgcaaagg ccgagggtaa tcagccaaat cgcgttaagc
    25321 ggggagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc aaccgcgcca
    25381 gtcagtacag tgaagcccgc ggctgccgta tacagactac caaccgcgcc gccaattccg
    25441 gctatccagc cacttagcgc tttttgaaaa gagaaatgcc agaacagcgg cggcgacaaa
    25501 ccatgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    25561 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    25621 ctgacgcggg cgatatcggt gttatccacc agatgcaggg ctttagtcgg gcacatccgc
    25681 acacaggccg gaccttgttc atcaaagcta caaaggtcac atttgacggc gatcgcgcga
    25741 atacctggca cccagtcaag caatgtgctg acacgcgccg gagcaggcgg tgccggtggc
    25801 gctttcgggg tattggcgtt tgccggaata tccagcggac ggctgccgga aaattcaatt
    25861 gcgccaaacg ggcaggcgat gccgcacagt ttgcagctta cgcacagact ttcattcaac
    25921 tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca
    25981 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattgagcat cactctcagg
    26041 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaggtg
    26101 tggcagccga tacagagcgt ggagtcggca attacaaaac gattcaccag gcattcctca
    26161 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    26221 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    26281 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatggcggt
    26341 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    26401 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    26461 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    26521 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    26581 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    26641 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    26701 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttactcgttt aacagagatg
    26761 ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga gatttttaag
    26821 ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    26881 cgcagggata atcaacactg gcgcaattat tgcttgtagc tggcaatagt taatgggagg
    26941 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    27001 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    27061 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    27121 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    27181 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    27241 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    27301 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    27361 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgcaga tgaaaatcac
    27421 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    27481 tcatggcgaa gcgggcactc acacaccggg catgagccag cgtcggatgc tggaagtcga
    27541 aattgacgtg ctggacaaaa acaaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    27601 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    27661 gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc gcggttattg aaggcgacca
    27721 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggt acaccagcga ttcaggtgaa
    27781 caccggtaaa ggctgccatc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    27841 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    27901 cttcgatctc ggtgaaaaac acaaagtagc ggtgctttcc gttaccgaag gtgaagacaa
    27961 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    28021 cctgttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgccc gcgaagtcaa
    28081 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    28141 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    28201 ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    28261 ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt tcacgttggc
    28321 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    28381 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    28441 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    28501 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgtggcg
    28561 gtcataccca cgccattttt aaattcggcc tcgaccagtt actgcctgaa aacgttgagt
    28621 ttatccacgg tccgggttgc ccggtgtgcg tactgccgat gggtagaatc gacacctgcg
    28681 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac
    28741 cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    28801 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgcaaa gtggtgttct
    28861 tcggcttagg ttttgaaacc accatgccga ccaccgccat cactctgcaa caggcgaaag
    28921 cgcgtgatgt gcagaatttt tacttcttct gccagcacat tacgcttatc ccgacgctgc
    28981 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    29041 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    29101 tggtcgctgg tttcgaaccc cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga
    29161 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg ggtggtaccg gatgccggta
    29221 acctactggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac agcgaatggc
    29281 gcggcttagg cgtgattgaa tcttctggcg tgcacctgac gccggattat caacgattcg
    29341 atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg cgcgcgcgtt
    29401 gtggtgaggt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    29461 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    29521 ggtatcagta tcgtcagcag gagagtgaag cgtgaataat atccaactcg cccacggcag
    29581 cggcagccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    29641 gtggctggca gagcaggaag atcaggcacg tcttgatctg gcgcagctgg tagcggaagg
    29701 cgaccgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    29761 taatatcggc aagctggcga tttgcggcac cgcgaatgac gttgcgggca gtggcgctat
    29821 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    29881 agccgtagtg accagcatgg cagaaaccgc ccgcacggca ggcattgcca tcgttactgg
    29941 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    30001 gggcgcaatt ccggcgaata ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt
    30061 gctggttagc ggtacactcg gcgaccacgg ggcgactatc cttaacctgc gtgagcagct
    30121 ggggctggat ggcgaactgg tcagcgactg cgcggtgctg acgccgctta ttcagacgct
    30181 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt
    30241 ggttcatgag ttcgcggcag cctgcggttg tggtattgaa atttctgaat cagcgctgcc
    30301 ggttaaacct gccgtgcgcg gcgtttgcga attgctggga ctggacgccc tgaactttgc
    30361 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggcagc
    30421 gttacattcc catccactgg ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa
    30481 aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc ctcgatttac cacacgccga
    30541 accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    30601 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    30661 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    30721 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    30781 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcgtcgcgt gaaaaagaca
    30841 cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc agcattttgt
    30901 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    30961 ccgcaggtgg gctatacccc aaatttggtc actattgcct gatgccactg gcggcgggag
    31021 ggcatatttt tggtggctgt gaatttattc gttatgacga gcgtccctgg agcgaaaaag
    31081 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    31141 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    31201 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    31261 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagcatt gtcttacgca
    31321 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    31381 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    31441 aagagatgct gctgatcaat ctctacgagc gggacgattt agccccctat gaacgcatgt
    31501 tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    31561 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    31621 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    31681 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    31741 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    31801 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    31861 gtgaaactgg ctcgggtaaa gagctgattg cccgtgcgat ccataatctc agtgggcgta
    31921 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg
    31981 atctgtttgg tcatgagcgt ggggctttta ccggtgccaa cgcccagcgc attggtcgtt
    32041 ttgaactggc ggataaaagc tctctgttcc tcgacgaagt gggcgatatg ccactggagt
    32101 tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca
    32161 aaatcattca gacggacgta cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg
    32221 tcgccgaccg agagttccgt agcgatctct attaccgcct gaacgtattc ccgattcacc
    32281 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    32341 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ctgcgcacct
    32401 tgagc
//
LOCUS       DAJTYH010000039        31532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTYH010000039 DAJTYH010000000
VERSION     DAJTYH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(433..639)
                     /locus_tag="OO609_001798"
     CDS             complement(433..639)
                     /locus_tag="OO609_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6633247.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            952..1041
                     /gene="kdpF"
                     /locus_tag="OO609_001799"
     CDS             952..1041
                     /gene="kdpF"
                     /locus_tag="OO609_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6633248.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1041..2714
                     /gene="kdpA"
                     /locus_tag="OO609_001800"
     CDS             1041..2714
                     /gene="kdpA"
                     /locus_tag="OO609_001800"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6633249.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2737..4784
                     /gene="kdpB"
                     /locus_tag="OO609_001801"
                     /pseudo
     CDS             2737..4784
                     /gene="kdpB"
                     /locus_tag="OO609_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4793..5365
                     /gene="kdpC"
                     /locus_tag="OO609_001802"
     CDS             4793..5365
                     /gene="kdpC"
                     /locus_tag="OO609_001802"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6633250.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5358..8038
                     /gene="kdpD"
                     /locus_tag="OO609_001803"
                     /pseudo
     CDS             5358..8038
                     /gene="kdpD"
                     /locus_tag="OO609_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8035..8720
                     /gene="kdpE"
                     /locus_tag="OO609_001804"
                     /pseudo
     CDS             8035..8720
                     /gene="kdpE"
                     /locus_tag="OO609_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8995..9099
                     /locus_tag="OO609_001805"
     CDS             8995..9099
                     /locus_tag="OO609_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6633251.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9410..11607
                     /gene="speF"
                     /locus_tag="OO609_001806"
                     /pseudo
     CDS             9410..11607
                     /gene="speF"
                     /locus_tag="OO609_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11604..12923
                     /gene="potE"
                     /locus_tag="OO609_001807"
     CDS             11604..12923
                     /gene="potE"
                     /locus_tag="OO609_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6633252.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12979..14619)
                     /gene="pgm"
                     /locus_tag="OO609_001808"
     CDS             complement(12979..14619)
                     /gene="pgm"
                     /locus_tag="OO609_001808"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6633253.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14645..15190)
                     /gene="seqA"
                     /locus_tag="OO609_001809"
     CDS             complement(14645..15190)
                     /gene="seqA"
                     /locus_tag="OO609_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6633254.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15375..16139
                     /gene="ybfF"
                     /locus_tag="OO609_001810"
     CDS             15375..16139
                     /gene="ybfF"
                     /locus_tag="OO609_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6633255.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16279..16572
                     /gene="ybfE"
                     /locus_tag="OO609_001811"
     CDS             16279..16572
                     /gene="ybfE"
                     /locus_tag="OO609_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6633256.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16712..17242
                     /gene="fldA"
                     /locus_tag="OO609_001812"
     CDS             16712..17242
                     /gene="fldA"
                     /locus_tag="OO609_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6633257.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17452..17538
                     /gene="uof"
                     /locus_tag="OO609_001813"
     CDS             17452..17538
                     /gene="uof"
                     /locus_tag="OO609_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6633258.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17531..17977
                     /gene="fur"
                     /locus_tag="OO609_001814"
     CDS             17531..17977
                     /gene="fur"
                     /locus_tag="OO609_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6633259.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18061..18387)
                     /locus_tag="OO609_001815"
     CDS             complement(18061..18387)
                     /locus_tag="OO609_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633260.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18424..19830)
                     /gene="chiP"
                     /locus_tag="OO609_001816"
     CDS             complement(18424..19830)
                     /gene="chiP"
                     /locus_tag="OO609_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6633261.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20409..22073)
                     /gene="glnS"
                     /locus_tag="OO609_001817"
     CDS             complement(20409..22073)
                     /gene="glnS"
                     /locus_tag="OO609_001817"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6633262.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22276..24222)
                     /gene="nagE"
                     /locus_tag="OO609_001818"
     CDS             complement(22276..24222)
                     /gene="nagE"
                     /locus_tag="OO609_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6633263.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24555..25355
                     /gene="nagB"
                     /locus_tag="OO609_001819"
     CDS             24555..25355
                     /gene="nagB"
                     /locus_tag="OO609_001819"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6633264.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25415..26563
                     /gene="nagA"
                     /locus_tag="OO609_001820"
     CDS             25415..26563
                     /gene="nagA"
                     /locus_tag="OO609_001820"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6633265.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26572..27792
                     /gene="nagC"
                     /locus_tag="OO609_001821"
                     /gene_synonym="nagR"
     CDS             26572..27792
                     /gene="nagC"
                     /locus_tag="OO609_001821"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6633266.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27840..28592
                     /gene="nagD"
                     /locus_tag="OO609_001822"
                     /pseudo
     CDS             27840..28592
                     /gene="nagD"
                     /locus_tag="OO609_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28989..30653
                     /gene="asnB"
                     /locus_tag="OO609_001823"
     CDS             28989..30653
                     /gene="asnB"
                     /locus_tag="OO609_001823"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6633267.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31032..31108
                     /locus_tag="OO609_001824"
     tRNA            31032..31108
                     /locus_tag="OO609_001824"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31066..31068,aa:Met,seq:cat)
     gene            31117..31201
                     /locus_tag="OO609_001825"
     tRNA            31117..31201
                     /locus_tag="OO609_001825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31151..31153,aa:Leu,seq:tag)
     gene            31225..31299
                     /locus_tag="OO609_001826"
     tRNA            31225..31299
                     /locus_tag="OO609_001826"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31257..31259,aa:Gln,seq:ttg)
     gene            31334..31408
                     /locus_tag="OO609_001827"
     tRNA            31334..31408
                     /locus_tag="OO609_001827"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31366..31368,aa:Gln,seq:ttg)
     gene            31424..31500
                     /locus_tag="OO609_001828"
     tRNA            31424..31500
                     /locus_tag="OO609_001828"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31458..31460,aa:Met,seq:cat)
BASE COUNT         7513 a         7963 c         8209 g         7847 t
ORIGIN      
        1 taacatctta ttggattaga tatgttaaca attaattatt cttaatatat caataaagta
       61 tatattactc tctttactat ctgtttttat tgcggtagcg atactaataa caaaattcgg
      121 aatttattta gatgctggaa ttagaaaaag ggatgacggc tttctcacct taccctgaag
      181 tttcacgaaa gtgtgtaagg aagtatgcaa tctaactgga gatttctgat gatctagtcg
      241 cttagagcga caatgaaggt gaccgcatcc tgggtagccc tatgaaagga tactattgaa
      301 acgtgatgct aaaacagtga tgaaatgaat aatacgatgg aaacgtcttc catgttaatt
      361 atcatggaag acgttgatgt tgaacaccta aaccagcccc ctgatacgag gctggcttaa
      421 gcgcgcaggc aatcagtaaa aatcaccggt tgccttttcg gcctgatcca tccacaccgg
      481 tttatcgctg gttttcgacc agacgcggtg caggtagctg taaaagcggg cgcggtcttt
      541 ccagaaaagc atgaaaggca gcgccagaac gcccgctgca accgcatatg tacggcgtaa
      601 aaagataagc catgcaggat attctctgta gagttccata cttttctccc ccttttgtat
      661 ctacccggtg aatagcaccg gaaaaatgaa tttgtttatc tgatgaaaat agtaccgcct
      721 tttgtgtaat tttactactc atccgaccac ttatttttgc ttattgatgg tttatttaca
      781 ttcacactgt aattaaatta cacaaaagtt aaattaatac taaccatcag ttaaatcatg
      841 gcctttgcca tttttatact ttttttacac cccgtccgca gatttttgcg aaatctttgc
      901 agccagaatt ctactcttcc ggtatcactt ttaggccact ggaggtgcac tgtgagtgca
      961 ggcgtgatag ccggcgtgtt gctggtgttt ttattactgg gctatttggt ttatgccctg
     1021 atcaatgcgg aggcgttctg atggctgcgc aagggttctt actgattgcc acgtttttac
     1081 tggtattaat ggtgctggcg cgtcctttag gcagcgggct ggcgcggctg attaatgaca
     1141 ttcctcttcc aggtacagcg ggcgttgaac gtatactctt tcgtttacct ggcgtctctg
     1201 accatgagat gaactggaag caatatcttt gtgccattct cggcctgaac atgctggggc
     1261 tggcggtgct gttttttatg ttgctcggtc agcactatct gccgcttaat ccacagcagt
     1321 tgccggggct gtcgtgggat ctggcgctga ataccgccgt cagctttgtc acgaatacca
     1381 actggcaatc ttatagcggt gaaaccacgt tgagctattt cagccagatg gcaggcttaa
     1441 cggtacaaaa ctttctttct gccgccagcg ggattgcggt gatttttgcc ttcatccgtg
     1501 cgtttactcg ccagagcatg agcacactcg ggaatgcctg ggtcgatctg ctacgcatca
     1561 cgttatgggt gctagtccct gtggcgttgt tgatcgcact gttttttatt caacaaggtg
     1621 cgcagcaaaa ctttctgccc tatcaggcag tgaataccgt tgaaggagcg caacagctgt
     1681 tacccatggg accagtagct tctcaggaag cgatcaagat gctcggtact aacggcggtg
     1741 gcttctttaa tgccaactcg tcgcatccgt ttgaaaaccc aaccgcactg accaacttcg
     1801 tgcagatgct ggcgatcttc ttgatcccaa cagcgctgtg ctttgccttt ggtgaagtga
     1861 cgggcgatcg ccgccagggg cgcatgttgc tgtgggcgat gtcagtgatt tttgtcatct
     1921 gcgtaggcgt ggttatgtgg gcagaagttc agggtaatcc tcatctgctg gcgctcggcg
     1981 cagacagcag catcaatatg gaaggtaaag agagccgttt cggcgtgctg gtcagtagcc
     2041 tgtttgcggt cgtgactacg gcggcgtcct gtggcgcggt gattgcgatg catgattcgt
     2101 ttaccgctct cggcggcatg gtgccgatgt ggctaatgca aattggtgaa gtggtgttcg
     2161 gcggtgtcgg ttctggtctt tacggcatga tgctgtttgt cctgctggcg gtgtttattg
     2221 ccgggctgat gattggtcgt acaccggaat atctgggtaa aaaaatagac gtacgcgaga
     2281 tgaaactgac tgcactggcg attctggtta ctccaacact ggtactgatg ggcgcggcgt
     2341 tggcgatgat gaccgacgcc ggacgtagcg ccatgcttaa ccctagcccg catggtttta
     2401 gcgaagtgct gtacgccgtg tcgtccgccg ctaacaacaa cggcagcgcc tttgccggat
     2461 taagcgccaa ctctccgttc tggaactgtt tactggcgtt ctgcatgttt gtcggtcgct
     2521 tcggggtgat tatcccggtg atggcaattg ccggttcgct ggtgagtaaa aagagccaac
     2581 ccgccagctc cggcacgctg ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca
     2641 ccgtgttgct ggttggcgca ctgaccttta tccctgccct ggcgcttggt ccggtggcgg
     2701 aatatctctc ctgatgatat tgagtgagca ctgaatatga gtcgtaaaca actggcgcta
     2761 ttcgaaccaa cacttgtcgt tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag
     2821 gcgcaatggc gcaatccggt gatgtttatc gtctggatcg gcagtctgct gaccacctgt
     2881 attagcatcg cgatggcaag cggtgcgatg cctggcaatg cgctgtttag cgcggcaatt
     2941 agcggctggc tgtgggtcac cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc
     3001 cgcagtaaag cgcaggccaa cagtcttaaa ggggtgaaaa aaactgcctt tgcccgcaag
     3061 ctgcgtgagc cgaaatatgg cgctgcggcg gacaaagttc ctgccgatca actgcgtaaa
     3121 ggtgacatcg tgctggtaga agctggcgat attatcccct gcgatggtga agtcattgaa
     3181 ggcggcgcat cggtcgatga aagcgccatc accggggaat cggcaccggt gatccgtgaa
     3241 tccggcggcg attttgcctc cgttaccggc ggcacgcgta ttctttctga ctggctggtg
     3301 attgagtgta gcgttaaccc cggtgaggca tttctggatc ggatgatcgc gatggtggaa
     3361 ggcgcacagc gacgcaaaac accgaacgag attgccctga ctatcctgct gattgctctg
     3421 accatcgtct ttttactggc aaccgccacg ctgtggccgt tttccgcgtg gggcggtaat
     3481 gccgtgagtg taacggtact ggtggcgctg ctggtctgtc tgatcccaac cactattggt
     3541 ggcctgttgt cagcgatcgg cgtcgccggg atgagtcgga tgctgggcgc gaatgtgatt
     3601 gccaccagcg gacgtgcagt tgaagcggca ggtgacgttg acgttctgct actggataaa
     3661 accggcacca tcacactcgg taaccgtcag gcgtcggagt ttatccccgc gcagggcgtg
     3721 gatgaaaaaa cgctggctga cgccgcacaa ctggcttcgc tggctgatga aacgccggaa
     3781 ggtcgcagta ttgtgatcct cgccaagcaa cgttttaacc tgcgcgagcg cgatgtgcag
     3841 tcgctccatg ccacctttgt accgtttact gcgcaaagcc ggatgagcgg gatcaacatc
     3901 gacaaccgca tgatccgtaa aggttctgtc gatgccattc gccgccatat tgaggctaac
     3961 ggcggtcact tccctgccga tgttgatcaa aaagtcgatc aggttgcgcg tcaaggagcc
     4021 acgctgctgg tggtggtgga aggttctcgt gtgctgggcg ttattgcgct gaaagatatc
     4081 gtcaaaggcg gtattaaaga gcgcttcgcc cagctgcgca aatgggcatt aaaacggtga
     4141 tgattaccgg cgataaccgt ctgactgccg ccgcgatagc tgcggaagcg ggtgtcgatg
     4201 attttctcgc cgaagcgaca ccggaggcca agctggcatt gatccgtcag tatcaggcgg
     4261 aaggtcgttt ggtagcgatg accggcgaca gcaccaacga tgctacggcg ctggcgcagg
     4321 cagatgtcgc ggtggcgatg aactccggca cccaggcggc taaagaggcg ggcaatatgg
     4381 tggatctcga ctctaacccg accaagttga tcgaggtggt gcacattggc aaacagatgc
     4441 tgatgacccg tggctcgctg accaccttca gcattgccaa cgatgtggcg aaatacttcg
     4501 ccattattcc ggcggcgttc gcggcaacat atccgcagtt aaatgcgctg aacatcatgc
     4561 gcctgcattc gcccgactcc gccattctca gtgcggtcat tttcaacgcc ttgattatcg
     4621 tctttttgat tcctctggcg ttaaaaggcg tgagttataa accgcttacc gcttctgcca
     4681 tgttgcgccg taacttatgg atttacggtc tgggtgggct gctggtgccg tttattggta
     4741 tcaaagtcat tgatttactg ctaaccgttt gtagtctggt gtgaggttta ccatgagtgg
     4801 attacgtccg gcattatcga catttctctt tctgttattg attactggcg gcgtttaccc
     4861 gctgctgacc accgcactgg ggcaatggtg gtatccctgg caggccaacg gttcgttgat
     4921 tcgtgaaggt gatacggtgc gcggttcggc attaatcggg caaaatttta ccggcaacgg
     4981 ctattttcat ggtcgcccgt cggcaacagc agaaatgccc tataatccac aggcttctgg
     5041 cgggagcaat ctggcagtca gtaacccgga gctggataaa cagatagccg cacgcgttgc
     5101 cgcattacgg gccgctaacc cggacgccag cacgaacgtt ccggttgaac tggtgacggc
     5161 gtcggcaagc ggactggaca ataacatcac cccgcaagcg gcggcctggc agatcccacg
     5221 catcgcaaaa gcgcgtaatc tcagcgttga acagctcacg cagctgatcg caaaatacag
     5281 ccaacaaccg ctggtgaaat atatcggcca gccggtcgtc aacattgtcg aactcaatct
     5341 ggcactggat aagcttgatg aataacgaac ccttacgtcc cgaccccgac cgtctgctgg
     5401 aacaaactgc cgcgccgcat cgggggaagc tgaaagtttt cttcagtgcc tgtgcaggcg
     5461 tcgggaagac ctgggcgatg ctggcagaag cccagcgact gcgggcgcaa gggctggata
     5521 ttgtggttgg tgtggtagaa acccatgggc ggaaagatac cgccgccatg cttgaagggc
     5581 tggctgttct gcctctgaaa cgccaggcgt accgtgggcg gcatatcagc gagtttgatc
     5641 tcgatgccgc cctcgcccgc cgtccggcgc tgatcttaat ggacgaactg gcgcacagta
     5701 atgcgccagg ttctcgtcat cctaaacgct ggcaggatat cgaagaactg ctggaagctg
     5761 gcattgatgt tttcacaacc gtcaacgttc agcatctgga aagtctgaat gatgtggtca
     5821 gcggcgtcac cggaattcag gtacgggaaa ccgtgcccga tccttttttc gatgccgccg
     5881 acgacgtggt gctggtggac ttgcccccgg acgatctgcg ccagcggctg aaagaaggca
     5941 aagtctatat tgccgggcag gcggagcgcg ccattgaaca ttttttccgc aaaggcaatc
     6001 tgatcgccct gcgcgaactg gcactgcgcc gtactgccga tcgcgttgat gagcaaatgc
     6061 gcgcctggcg ggggcatcct ggcgaagaga aagtctggca cacgcgcgac gcgatccttt
     6121 tatgcatcgg ccataacacc ggcagcgaaa actggtccgc gcggcggcgc ggctgacgtc
     6181 acggctgggg agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc
     6241 ggagaaaaaa cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga
     6301 gacggcaaca ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca
     6361 taatctcggc aagattattc tcggtcgccc agcctcgcgc cgctggtggc gtcgggaaac
     6421 atttgctgac cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga
     6481 tgaaccaccc gcccgcacga ttaacaacgc gccggatagc cgctcattta aagacaagtg
     6541 gcgggtacag attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat
     6601 tgccatgcag tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg
     6661 cgtggtggtg gcgctatttt atggacgctg gccttcggtg gttgccaccg tcattaatgt
     6721 ggtgagtttc gatctctttt ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca
     6781 atatctgctg accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc
     6841 tggtgtgcgt tatcaggcgc gggtagcccg ttaccgcgag caacgcacac ggcacttata
     6901 tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag gacatcgccg ccaccagcga
     6961 acaatttatt gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg
     7021 taaattgcag ccgttaacac atccgcaagg aattacgcca tgggacgatg ccatcgcgca
     7081 gtggagtttt gataaaggcc tgcctgcggg cgcgggcacc gacacgttac ccggtgtacc
     7141 gtaccagatt ttgccgctaa aaagcggcga gaaaacctac gggctggtgg tggtggagcc
     7201 ggggaatctg cgccagttga tgatcccgga acagcagcgc ctgctggaga cttttacgct
     7261 gttagtcgcc aatgcccttg agcggctgac gctaaccgcc agcgaagaac aggcgcggat
     7321 ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggca gcgctttcgc atgatttacg
     7381 cacgccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga
     7441 aggatcaccc cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg
     7501 actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa
     7561 agagtggtta acgctggaag aagtagtcgg cagcgcgctg caaatgccgg aaccgggttt
     7621 atcgtcgccc atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc
     7681 actctttgaa cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca
     7741 ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat ctacaactgg atgtctggga
     7801 taacggcccc ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg
     7861 gaataaagag tcggcggtgc cgggtgtcgg gcttgggttg gcaatttgtc gggcgattgt
     7921 ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg gaaggtggtg cctgttttcg
     7981 tgttacactt ccccagcaaa ccgctcctga acttgaagac tttcatgagg atatgtgaca
     8041 aacgttctga ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac ggcgctggag
     8101 ggcgacggga tgcgcgtctt tgaggccgaa actctgcaac gcggcttgct ggaagcggca
     8161 acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag
     8221 tttatccgcg acctgcgcca gtggagcccg gtgccggtga ttgtgctttc cgcacgcagc
     8281 gaagagagcg acaagatcgc cgcgctggat gccggagcgg atgattatct gagtaagccg
     8341 tttggtattg gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccact
     8401 ccgccaccgc cgcgcccgat ccgctggtga aattttccgg tgttaccgtc gatttagccg
     8461 cccgcgtgat tcaccggggt gaggaagagg tgcatctcac accaattgag ttccgcctgc
     8521 tggcggtgct gctcaacaat gccggaaaag tactcaccca gcgccagctc cttaaccagg
     8581 tgtgggggcc aaacgcggtc gaacacagtc actatttgcg tatttatatg ggacatctgc
     8641 gacaaaaact ggaacaggat ccggcccggc ctcgccactt cattactgaa accggtattg
     8701 ggtatcggtt tatgctttga attgttattc aataaattcg cttaaatagt gattaattta
     8761 ttattaaacc tgcgccgaat attattattt ggcaggttta atttcttaac gcaaatataa
     8821 aacacgaaat tacaacattt aagtaacaaa acagcatcta atatggcgat attcataatt
     8881 aatgatttat tatctggggt atgatcgttt tttgttgatc ttcttcacag attatagcca
     8941 tttcatggat agaataactc taccttcaac tgacacagca agcggtaaag gtaaatggaa
     9001 aataataacc gcttaatgcc tcatataagg cggacaaccc atatcatgaa gtttgcccat
     9061 cgtaatagtt ttgactttca tttctttaat gcccgctagt cttctgacta aagggcaccc
     9121 caacgtacag gtctccctga ctttaagcat tacaggttaa tacctgtatt cctcggtgct
     9181 catatactgc taaccctttt aaactctaaa taattcgagt cgcagcactt gcaacttgag
     9241 gtatgacgag tatagccagt taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa
     9301 ctactgattt atttatcagc ggtggagctt tgcttttttt ccgacgtgat cgatttctcc
     9361 tttgagaaat tgaggacctg ctattacctg aaataaagag atgaataaaa tgtcagaatt
     9421 aaaaattgcc gttagccgtt cttgcccgga ttgtttttcc actcatcgtg catgcgtgaa
     9481 tattgacgaa agtaattata ttgacgttgc cgccattatt ttatcagtca atgatgttga
     9541 acgtggaaaa ctcgataaaa tagacgctac tggctatggc attcctgttt ttattgcaac
     9601 ggaaaatgaa gaacgtgtac cagcagaata tctgccacgt atttctggtg tcttcgagca
     9661 ttgcgaatca cggaaagagt tttatggacg tcagttagaa accgctgcta gccattatga
     9721 aacgcaactg cgcccgccct tcttccgcgc actggtcgat tatgtcaatc agggcaatag
     9781 cgcttttgac tgcccgggtc atcagggagg tgagtttttc cgtcgtcatc cggcagggaa
     9841 tcagttcgtg gagtacttcg gagaaacgct gttccgttcc gacttatgca acgcagacgt
     9901 agcaatgggc gatctgctga ttcacgaagg cgcgccatgc attgcacagc aacatgcggc
     9961 aaaagtgttt aatgccgata aaacatactt cgttctaaat ggcacttcat cttctaacaa
    10021 agtggtttta aacgccctgc taacaccggg tgatctggtg ctgtttgatc gcaataacca
    10081 caaatctaac caccacggag cgttgctaca ggctggtgca acaccggttt atctggaaac
    10141 ggcacgtaac ccgtatggct ttatcggtgg cattgatgcg cactgttttg aagaaagtta
    10201 cctgcgtgag ctgatcgcgg aagtcgcacc gcagcgggca aaagaggctc gtcctttccg
    10261 cctcgctgtg attcagttag gcacctacga cggtacgatt tataacgccc gccaggtggt
    10321 ggataaaatt ggtcatctgt gtgactacat tctgtttgac tccgcatggg ttggctatga
    10381 gcaatttatc ccgatgatgg ctgactgttc gccgctgttg ctggatctta atgagaacga
    10441 tccgggtatt ctagttacgc aatccgtaca taaacagcag gctggttttt ctcaaacttc
    10501 acaaatccat aaaaaagaca gccacatcaa agggcaacag cgttatgtac cgcacaaacg
    10561 catgaacaac gcctttatga tgcacgcctc caccagcccg ttctatccgc tgtttgccgc
    10621 actggatatc aacgccaaaa tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg
    10681 tgtggtaaat ggcattaatg cccgcaaact gatcctcgat aactgtcagc atattcgtcc
    10741 gttcgtacct gaactggtgg atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc
    10801 ggttgatctg cgcttcttcc agtttgtacc aggggaacac tggcattctt ttgaaggcta
    10861 tgcagagaat caatactttg tcgatccttg caaactgttg ctgacaaccc caggtattga
    10921 tgcacgtaac ggcgaatatg aagcgttcgg tgtacccgcg acgattcttg ctaacttcct
    10981 gcgcgaaaat ggcgtagtgc cggaaaaatg cgatcttaac gccatcctct tcctgctgac
    11041 tccggcagaa gatatggcca aacttcagca acttgtgccc tgctggtacg cttcgaaaaa
    11101 ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa acagcatgaa
    11161 gagcgctacg ccggttatac cctgcgtcag ttgtgccagg aaatgcatga tttgtatgcc
    11221 cgccacaacg tgaaacaact gcaaaaagag atgttccgca aagcgcactt cccacacgtc
    11281 agcatgaatc cgcaagaagc caactacgcc tatttacgcg gtgaagtgga gctggttcgt
    11341 ctaccggatg ccgaaggtcg tatcgctgcc gaaggggcgc ttccttatcc tccgggtgtg
    11401 ctgtgtgttg ttccgggtga aatctggggt ggtgctgttt tgcgttactt cagcgctctg
    11461 gaagaaggga tcaacctgct gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa
    11521 gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc
    11581 accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc
    11641 agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa
    11701 agcttgccga agttgggaca atttcaatta tctcctggct ggtgacagcc gtaggctcaa
    11761 tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggtggtatgg
    11821 gcggttatgc cgagtatgct ttcggtaaat ccggtaactt tattgcgaac tatacctacg
    11881 gcgtctcact gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg
    11941 aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggggtgctgt
    12001 ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta
    12061 ccgtgtgggg gggcattatt ccggtggtag gcctgtgcat tatcggctgg ttctggttta
    12121 gcccgacgct ttacgttgat tcctggaatc cgcatcatgc accgttcttc agtgcagtag
    12181 gttcttccat cgctatgacg ctgtgggctt ttcttggtct ggagtctgcg tgtgcgaata
    12241 ctgatgtagt ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag
    12301 gtgcggcggt gatttatatc gtctccacca acgtgattgc agggattgtg cctaatatgg
    12361 agctggcaaa ttcaacggca ccatttggcc tggccttcgc acagatgttt acaccagaag
    12421 tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcgctg cttggctggc
    12481 agttcaccat tgcccaggtg tttaaatctt catctgatga aggctacttc cctaaaattt
    12541 tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt gtgattatcc
    12601 agagcgggtt ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg
    12661 ttaacctggc cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca
    12721 ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac tttgttgctt
    12781 ttgttggcgc gatgtatagc ttttatgcgc tgtactcatc cggggaagaa gccatgctgt
    12841 acggttccat cgtgaccttc cttggttgga cactgtatgg tctggtatca ccacgctttg
    12901 aactgaaaaa taaacacggt taaaaaagca aagtaaaaaa gggcgatctt gcgaccgctc
    12961 tttttttatt aaatgtgttt acgcgttttt cagaacttcg ctaacaatct caaccgcttc
    13021 tttctcaatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga tcttatatgc
    13081 gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc
    13141 gccaatagaa gcaccgttgc ccggagcagc agtcaggcgc gcggtgatcg ggtcacctgc
    13201 cagggtgctg gcgctcacca tttccggaga cagcttagac agcgccgctt tttgtgcgga
    13261 agtcgcagct gcctgcaaac ggttgtagct cggcgcacca aagcgttttg ccagttcgtt
    13321 gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca gcagacacat
    13381 aatgatgccg tctttgtcgg tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc
    13441 tgcgctctct tcgccgccaa agccgaagct gccgtcgaac agaccatcaa caaaccattt
    13501 gaaacctacc ggtacttcta ccagcttacg gcccaagtca ttgaccacac ggtcgatcat
    13561 cgcagaagaa accagcgttt taccgacagc aacatctttg ccccactgcg gacgatgctg
    13621 gaacaggtag ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac ctgccggagt
    13681 gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga acttatcacg
    13741 cagtgccagc agacccgcca tcgcacactc ggaggagcag tccatacgga tcgcaccgtc
    13801 tttatcgagg tgcataaagc ggaaggtttg atcgacctga tcgttaacga tggtcaggtt
    13861 gaggttgtaa tactcgccaa tgcgcttcca gtattcgata ccggaaccgc ccaacggatc
    13921 aacgcccagc gtcaggcccg ctttctgaat cgccgccata tcaacgatat cggccagccc
    13981 ttccacgaac ggctgcacca gatcctgctc tttcacatag ccggatgcca tcgcttcgtc
    14041 gagggagata cgcttcacgc ctttcaggcc atcggccatc agtgcgttgg ccttgtcttc
    14101 caccactttg gtgacgttag tatcagccgg gccaccattt ggcggattgt acttgatacc
    14161 accatcttcc ggcgggttat gggacggtgt aatcacgata ccgtctgcca gcgggccacc
    14221 ttttttattg tgaaccagga tggcattgga aacggcaggc gtcggagtga agccattgtt
    14281 ttcctgcaca atgacatcaa cgccgttcgc tgccagcact tccagtacgg aaatgaacgc
    14341 aggctcggac agggcgtgag tatctttacc cacatagcaa gggccagtga tgccgttttt
    14401 cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt taaagctgtg
    14461 gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct ccgcattccc
    14521 tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact
    14581 ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct ttgtctgcaa
    14641 cgttttagat agttccgcaa accttctcaa tcaattccgc cgggaattgc atcgactgca
    14701 tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc acccaatacg
    14761 gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc gtttgttcat
    14821 ctgccgcaaa gtacacgcgt gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct
    14881 gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc gctcgctttt
    14941 gctctgcgta ttcgtccgaa agcagaagtt cacgcattgc gcgaaccttg tctttaatcg
    15001 ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg
    15061 gagcagcagg ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc
    15121 tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca tcaacttcaa
    15181 tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca agagtatatg
    15241 aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg gaataaacct
    15301 tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga caacgcgaaa
    15361 aaaagaactt tgccatgaaa ttaaatatcc gcgcgcaaac tgcacaaaac cagcacaata
    15421 attctcccat cgttcttgtc catggtctgt ttggtagcct cgacaacctt ggcgtactgg
    15481 ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac cacggtcttt
    15541 caccgagaga gccggtaatg aattacccgg cgatggcgca ggatcttgtt gataccctgg
    15601 atgcactgca gatcgacaaa gcaacattta tcggtcactc catgggcggt aaagcggtaa
    15661 tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc gatatcgcac
    15721 cggtcgacta tcacgtacgc cgtcatgatg agattttcgc agctatcaac gcggtcagtg
    15781 aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat cttaatgaag
    15841 aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc
    15901 cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg gcatgggatc
    15961 accctgccct gtttatccct ggcggcaatt ctccgtatgt tagcgagcag taccgtgatg
    16021 atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg
    16081 tccatgctga aaaaccggat gcggtattac gcgctatccg tcgctatctc aatgattaac
    16141 gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc
    16201 atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct gattgtttcc
    16261 cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt agatctgttc
    16321 gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga tgaacagctg
    16381 cgtattaata aacgcaacca gctaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa
    16441 ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggaatc gcgtaatatg
    16501 agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct gcgtagtcag
    16561 ggcatcgttt aaatttccac tttcatgtag cacagtgtgc agtcctgctc gtttgctgat
    16621 tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt tccaccccca
    16681 tttcaataag tttcaagagg ttatttcact catggctatc actggcatct ttttcggcag
    16741 cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt
    16801 tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt acgacattct
    16861 gctgctgggc atcccaacct ggtattacgg cgaagcgcag tgtgactggg atgacttctt
    16921 cccgactctc gaagagattg atttcaatgg taaactggtt gcgctgtttg gttgtggtga
    16981 ccaggaagat tacgccgaat atttctgcga cgcattgggc accatccgcg acatcattga
    17041 accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt tcgaagcatc
    17101 aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag accgtcagcc
    17161 ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag agttgcatct
    17221 cgacgaaatt cttaatgcct gatgtgatgc ggcgtagact catgtctacg ccgtattaat
    17281 agataatgcc aatcaaaata attgctacaa atttgtaact tttgctgttg tacctataca
    17341 atgtcccagt gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta
    17401 acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat aatgatacgc
    17461 attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt agtaacagga
    17521 cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctga aagtaacgct
    17581 tcctcgttta aaaatcctgg aagttcttca ggagccggac aaccatcacg tcagtgcgga
    17641 agatttatac aaacgtctga tcgatatggg tgaagaaatt ggtctggcta cggtatatcg
    17701 cgtactgaac cagtttgacg acgctggtat cgtcacccgc cacaattttg aaggcggtaa
    17761 atccgtattt gaactgacac agcaacatca ccacgatcac ctgatttgcc tcgactgcgg
    17821 caaggttatc gaatttagtg atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa
    17881 acatggcatt cgcctgacta accacagtct ctatctttac ggtcactgtg ccgaaggcga
    17941 ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc agcctgaacg agaaaagcca
    18001 acctgcgggt tggctttttt atacaaggga aataagaaaa gcgagagtta cagctctcac
    18061 ttatttgtta ttactgcgta tttcctgcca gaccttatca caatcggctt tcaccgcctg
    18121 atcatttccg gtttgcgttg cacgcagaca ctgctgataa tccagtacgc gtacactttc
    18181 ctggtcggca aactgctgat gttgtttctc tttcttcagc acgtttaaca cgctctggca
    18241 ggcttcaatt ttttctggcg aaccttgcgc ggtgttgata caggcgctgt atgcctcttt
    18301 cagacggctg tcttcctgtg gtgcggttga ttgcgcacaa gctaccagcc ccgatgccat
    18361 catggcgatg agaatcagtt ttttcattga atgcggcagg cacaaaacct gccgcgtcgg
    18421 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    18481 gatgttaccg taaccgccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    18541 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    18601 gctgtaggca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    18661 ccaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg
    18721 aagattccag tttttcaggt catacatcgc actgaagaag accgcttttt cgccgttagc
    18781 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggt
    18841 tggggtcata cgttgcagga agtatccctg ctgactgtca gccttaatcc aggtgccttc
    18901 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    18961 ggtgccgtca taaagatcgt tgacgctgcg atcgtcaact ttatcgcggg taccatagaa
    19021 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    19081 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    19141 gaagtcgtat ttcgcaccga tggagtgcag ataatcaact ttggtggttt tatcgttctg
    19201 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    19261 gaaactcaag gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    19321 agtacccggc ataaagctcc agtgcggcgc taacagcgtt tgaccggttg gctgaatgta
    19381 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    19441 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    19501 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acgcagcaat
    19561 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    19621 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    19681 acgctgccag taatagatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    19741 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcaatag ccagcgccag
    19801 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    19861 gcaccaaagg tgaacagcga aaaaaaataa aaattaaaaa agtagcgttg agaaaagtct
    19921 cgctgactac aggaacagac tatttttcgc gacgcgaatt atcaatctct ttctgtaacc
    19981 agaatgtaag aacatgagca accgcacata ttttgatgat ttttgttaca aggcttaagt
    20041 ttgcggttga aaggcttgca gaagaataaa aacgtgaaat tccagcaaga tgaaagcggt
    20101 tacattttat ttaattatgt tttgtgattt taaatttatt ttatggataa attgcgaagc
    20161 ggaattaatt cgcgaagaaa agcagaaaaa acgccgcaga gcgcggcgtt tgggattgtt
    20221 tgctatttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag cacgcaaatt
    20281 caatatattg cagagatcat gtaggcctga taagcgaagc gcatcaggca atttagcgtt
    20341 tgaaatttat tgtctattta ttgccggatg cgacgtaaat gcctcacccg gcttggtaga
    20401 acttaaaatt actcgcctac tttcgcccag gtatcacgca gcccaacggt gcggttaaat
    20461 accggttttt cggccgtgga atggcggctg tcgaggcaga agtaaccttc acgctcaaac
    20521 tggaatgctt tacccgcaac cgcatctttc agcgacggtt cagcaaagcc ctgtttgatc
    20581 accagcgatt ccgggttaat caccgacagg aaatcatccg cagcacctgg gttaggcacg
    20641 ctgaacagac gatcatacaa acggatttca accggcagcg catgtgccgc gctcacccag
    20701 tgaataacac ctttgacttt acgaccatct gccggatctt tgcttaaggt gtcggcgtca
    20761 taagtacaga agatggtggt gatattacct tcggcatctt tctccacgcg ttcagcctta
    20821 atgacgtaag cattacgcag acgcacttct ttacccagca ccagacgttt gtactgcttg
    20881 ttagcttctt cgcggaaatc ggcgcgatcg atccaaattt caccgctaaa cggcacctga
    20941 cggctcccca tttccggttt gttcggatgg ttcggcatgg tgaccatttc gccttcgccc
    21001 tgatagtttt cgataaccag tttcaccgga tcgataaccg ccattgcgcg cggcgcattt
    21061 tcgttgagat cttcacggat gcaggattcc agcgacgcca tctcaatggt gttgtcctgc
    21121 ttggtcacgc cgatgcgttt gcagaactca cgaatagaag ccgcagtgta accacgacga
    21181 cgcagaccgg aaatggtcgg catacgcggg tcatcccagc cttcaacgtg cttgtcggtc
    21241 accagcaggt tcagcttacg cttggacatc acagtgtatt ccagattcag gcgcgagaat
    21301 tcgtactggc gcggatgaac aggaatggtg atgttatcca gtacccagtc gtacagacga
    21361 cggttgtcct ggaactcaag cgtacacaga gagtgcgtaa taccttccag cgcatcgctg
    21421 atgcaatggg tgaagtcgta catcgggtag atgcaccact tgttgccagt ctggtggtgt
    21481 tcagcaaatt taatgcggta cagcaccgga tcgcgcatta cgataaatgg cgacgccatg
    21541 tcgattttcg cacgcaggca ggctttacct tcttcaaaac caccggcacg cattttttcg
    21601 aacagcgcca agttctcttc aacgctgcgg tcgcggtacg ggctgttttt acccggctgc
    21661 gtcagggtac cgcggtattc gcggatctgt tccggtgtca gttcatcaac gtacgccagg
    21721 cctttattga tcagttcgat cgcataggcg tggagctgat caaaataatc ggaggagtaa
    21781 cggacgttac cagaccagtg aaaacctaac cactctacgt cgtttttgat cgaatcgaca
    21841 tactcgatat cttcttttac cgggttagtg tcgtcgaaac gcaggttgca ctggccttta
    21901 tagtcctggg cgatcccgaa gttcaggcag atagatttcg catggccaat atgcagatag
    21961 ccattcggct ccggcgggaa acgggtgtgt actgtgatgt gcttaccact ggccagatct
    22021 tcatcgatga tctgacggat aaagttagtc gggcgggctt ctgcctcact catcgtggat
    22081 tcctcaaagc gtaaacaacg tataacggcg tatgatctta taagcgggac aggctgacaa
    22141 ctgttagttt tttaaaattg ataatctaac aaggattatg gggatgattg atgaaaaaaa
    22201 aagcggcaga gaagatctcc accgcttgtc attgttggat gcgacgctgc cgtgtcgcat
    22261 caggcataaa gcagattact ttttgatttc atacagcggt gtttgacccg ccacaacatg
    22321 gccctgagct ttaatgagca agccactgaa atcgtcgata ttgctgcaaa ccaccgggct
    22381 aatcatcgag cgggcgttag cgttcaggta atccagatcc atttccagaa tcggttgccc
    22441 tgcgcttacc tgcgcaccct cttccaccag acgtttaaag cctttacctt ccagcgctac
    22501 ggtgtcgata cccatatgga cgacgatctc cgcgcctttt tcggtttcca ggcagaacgc
    22561 gtggttggtg ttgaagattt tcacgattgt ccctgcggct ggtgatacga cgattttatc
    22621 tgtcggtttc accgccacac cgtcacccac cgctttgctg gcgaatgctt cgtcaggaac
    22681 ctgatccagt gccacgacat caccggtaat cggcgatacc agctccgcga tagataccgc
    22741 gtttggtaca gcctgcggtt ttgctacagg cgcggcagtt gccggagttg cttcagctga
    22801 cgcagcggct accggaccac gggcaacgac tttcttcatc gcatcgccga tggattctgc
    22861 tttcgcgcca acaatcacct gaatagtctg tttgttcagt ttcactacac cagaagcacc
    22921 cagacgttta cacatcgtat cgttgacgcg ggcagagtca gccacggtca ggcgcagacg
    22981 ggtaatacag gcatcaatcg ctttcaggtt gtcagtaccg ccaaccgcag caatatagtt
    23041 ggttgccagt tgagtcagac cttcttcagt attgctgttg gcttcttcag taacgatctc
    23101 gtcttcttta tcttcacgac ccggcgtttt caggttgaac atgcggataa ccaaactgaa
    23161 caccacgaag tagatagcga agaagataac gcccatcacc agcagcatcc agacgttctg
    23221 gctggcggca ggcaggttat acatcaacgc gtagtcgata gcacccgcag agaaagagaa
    23281 gccggcatgg atacccagca gcgttgccac aaacaggctg ataccggtca gcagtgcgtg
    23341 caggaggtac agcagcggcg caaggaacat gaacaggaat tccagcggct cagtcacacc
    23401 ggtcaggaac gcagtaacag caacagaaag cagcatcccg ccaaccatcg gacgacgctc
    23461 tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc agaccgaaca tcatgatcgg
    23521 gaagaagccg gacatgaaca tccccgcggt gccgtcacca gcgtagaaac ggttgatgtc
    23581 gccgtggaaa accgtacccg ccgcgttggt gaattcacca atctggaacc aggcgatggt
    23641 gttcagcacc tgatgcagac cggttgggat cagcagacgg ttgatgaaac caaagatacc
    23701 ggaacccagc gcgcccgcag aaacgatcca ctcgccgcct gcatggatag cgtgctgtac
    23761 cggcggccag acgtaaccaa aaatggccgc cagcaccaga cagaagaagc cggtggcgat
    23821 cggcacaaag cgtttgccgc cgaagaagct caggaagtcc ggcagtttaa tatcggacca
    23881 acggttatag gctgcgccac caaccagacc ggtaatgata cccgccagta cacccatgtt
    23941 aatttctggg ttgatggtca ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc
    24001 cagcgccgcc gcacctgcgc tgtctttcga ccagctggat gccacaccga tggcgaagat
    24061 taatgcgagg ttatcaaaaa ttgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa
    24121 atctggctga ccgaatcgca gcaacagcgc cgccaccggc agcaccgcga tagggagctg
    24181 taacgcccta ccgagtcgct ggaaaaaacc taaaatattc atcttattcc ccctacgaga
    24241 accctatttg gctcgtttaa agccgtattt ttattttgct gcaaatttta ctgccgatgt
    24301 tctgtaatca gattgttaga tcatctgcta cagagtgtgt gaaaatttaa ttcgcatcgc
    24361 aaattaaacg cgtgtctttt gtgagttttg tcaccaaata tcgttatcat cactcccttt
    24421 tactggctaa accagaaaac ttattttatc attcaaaaaa tcaagtcgga ttgacgcctg
    24481 tctgcgcaaa tccaggttac gcttaaagat gtctaatccg ccaacggctt acattttact
    24541 tattgaggtg aataatgaga ctgatccccc tgactaccgc tgaacaggtc ggcaaatggg
    24601 ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc aactgccgat cgtccgtttg
    24661 tactgggcct gccgactggc ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc
    24721 ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt caacatggac gaatatgtcg
    24781 gtctgccgaa agagcatccg gaaagctact acagctttat gcaccgtaat ttcttcgatc
    24841 acgttgatat tccagcagaa aacatcaacc ttctcaacgg caatgccccg gatatcgatg
    24901 ccgagtgccg ccagtatgaa gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg
    24961 gcggtgtagg taacgacggt catattgcat ttaacgaacc ggcgtcttct ctggcttctc
    25021 gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc gaactctcgt ttctttgata
    25081 acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg tgttggtaca ctgctggatg
    25141 ccgaagaagt gatgattatg gtgctgggta gccagaaagc actggcgctg caggccgccg
    25201 ttgaaggttg cgtgaaccat atgtggacca tcagctgtct gcaactgcat ccgaaagcga
    25261 tcatggtgtg cgatgaacct tccaccatgg agctgaaagt taagacttta agatatttca
    25321 atgaattaga agcagaaaat atcaaaggtc tgtaattgtt atccctgccc tggttccttg
    25381 ctcagggcaa tattttttaa aatcgggggg cagaatgtat gcattaaccc agggccggat
    25441 ctttaccggc cacgaatttc ttgatgacca cgcggttgtt atcgctgatg gcctgattaa
    25501 aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa caacgttcac tgaacggggc
    25561 cattctctcc cccggtttta tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa
    25621 cgacaccgct gaagcggtca gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa
    25681 atcaggctgt actaactatc tgccgacgct tatcaccacc agcgatgagc tgatgaaaca
    25741 gggcgtgcgc gttatgcgtg agtacctggc aaaacatccg aatcaggcgt taggtctgca
    25801 tctggaaggt ccgtggctga atctggtaaa aaaaggcacc cataatccga attttgtgcg
    25861 taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt
    25921 gaccctggca ccggaaatgg ttcctgcgga agtcatcagc aaactggcaa atgccgggat
    25981 tgtggtttct gccggtcact ccaacgcgac gttgaaagaa gcaaaagccg gtttccgcgc
    26041 ggggattacc tttgccaccc atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc
    26101 gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat tgcggtatta tcgctgatgg
    26161 cctgcatgtt gattacgcca acattcgtaa cgctaaacgc cttaaaggcg acaaactgtg
    26221 tctggttacc gacgccaccg cgccagcagg tgccaacatt gaacagttca tttttgcggg
    26281 taaaacaata tactaccgta acggactttg tgtggatgag aacggtacgt taagcggttc
    26341 atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga
    26401 tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg attgacgttg aaaaacgtct
    26461 cggcacgctc gccgcaggta aagtagccaa cctgaccgca ttcacacctg attttaaaat
    26521 caccaagacc atcgttaacg gtaacgaggt cgtaactcaa taagagaaag tatgacacca
    26581 ggcggacaag ctcagatagg taatgttgat ctcgtaaaac agcttaacag cgcggcggtt
    26641 tatcgcctga ttgaccagta cgggccaatc tcgcggattc agattgccga gcaaagccag
    26701 cttgcccccg ccagcgtaac caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa
    26761 gaagttgatc agcaggcctc caccgggggc cgccgcgcta tctccatcgt caccgaaacc
    26821 cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcatg acgccaccat cactctgttt
    26881 gatctcagca gcaaagtgct ggcagaagaa cattacccgc tgccggaacg tacccagcag
    26941 acgctggaac atgccctgct gaatgccatt gctcagttta ttgatagcta ccagcgcaag
    27001 ctgcgcgagc taatcgccat ttcggtgatc ctgccagggc ttgttgaccc ggacagcggc
    27061 aaaattcatt acatgccgca tattcaggta gaaaactggg ggctggtaga agcactggaa
    27121 gaacgtttta aagtgacctg tttcgttggt cacgatatcc gtagtctggc gctggcggag
    27181 cactacttcg gtgcaagtca ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga
    27241 accggggccg ggattatctc taacgggcgc atttttattg gccgcaacgg taacgtcggt
    27301 gaaattggcc atattcaggt cgaaccgctg ggtgaacgct gccactgcgg caactttggc
    27361 tgcctggaaa ctatcgccgc caacgctgcc attgaacaac gggtgttgaa tctgttaaag
    27421 cagggctacc agagccgtgt gccactggac gactgcacca tcaaaactat ctgcaaagcc
    27481 gcgaacaaag gcgatagcct ggcctcggaa gtgattgagt atgtcggtcg tcatctgggt
    27541 aaaaccatcg ccattgctat caacttattt aatccgcaaa aaattgttat tgctggtgaa
    27601 atcaccgaag ccgataaagt gctgctccct gctattgaaa gctgcattaa tacccaagcg
    27661 ctgaaggcgt ttcgtactaa tctgccggtg gtacgttctg agctggatca ccgctcggca
    27721 atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg gtattttgct ccagcatttg
    27781 ctggaaaatt aatgtgcttt tatagtggcg cttattgttg tcaatattct gggtagtcca
    27841 tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg
    27901 taccgggtgc agcggaattt ttgcacggga ttatggataa aggcctgccg ctggtgttgc
    27961 tgaccaacta tccttcgcag actgggcaag atctggcgaa ccgctttgcc actgccggtg
    28021 tcgatgtgcc ggatagcgtg ttttatacct ctgcgatggc gaccgccgat ttcctgcgtc
    28081 gctaggaagg caagaaagcg tatgtggtgg gcgaaggcgc actgattcat gaactgtaca
    28141 aagccggttt cactattacc gatgtgaacc ctgatttcgt gattgttggc gaaacacgtt
    28201 cctacaactg ggacatgatt cataaagcag cctatttcgt cgctaacggt gcacgcttta
    28261 tcgccaccaa cccggacacc cacgggcgcg gtttttatcc cgcttgtggc gcgttgtgtg
    28321 cagggattga gaaaatctcc gggcgcaaac cgttctatgt tggtaagccc agcccgtgga
    28381 tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga agaaacagtg attgtcggcg
    28441 ataacctgcg taccgatatt ctggcgggct tccaggccgg tctggagacg attctggtgc
    28501 tttctggtgt ttcatcgctc gacgatatcg acagtatgcc gttccgccca agctggattt
    28561 acccgtcagt cgctgaaatc gacgttatct gaataccacc cacgtctgcg gacgtggttg
    28621 gctctgatga caaacgccaa actgcctgat gcgctacgct tatcaggcct acgcagctcc
    28681 tgcaatatat tgaattttca tgcttttgta ggccggataa ggcgttcacg ccgcatccgg
    28741 catgaacaaa tcgcacttta tcagcaattt ggcccccgta aaatataacc attcaataaa
    28801 acccgctaaa aattatcgat tcatcaataa atacatcctc acaccagacc ctttttcacc
    28861 aatcgacaat caccattacg ttttttattt ttgcactgcc aaatagcttg cgcacagtgc
    28921 tgctttgcgg cattttttta aacaagcaaa cacaacaagc aacaaatacc aggttaacgg
    28981 agaaggttat gtgttcaatt tttggcgtat tcgatatcaa aacagacgca gttgagctgc
    29041 gtaagaaagc actcgagctg tcacgcctga tgcgtcatcg tggcccggac tggtccggta
    29101 tttatgccag cgataacgcc attctcgctc acgaacgtct gtcaattgtt gacgttaacg
    29161 cgggggcgca acctctctac aaccaacaaa aaaaccacgt actggcggta aacggtgaaa
    29221 tctacaacca ccaggcattg cgcgctgaat atggcgatcg ttatcagttc cagacagggt
    29281 ctgactgtga agtgatcctc gcgctgtatc aggaaaaagg gccggaattt ctcgacgact
    29341 tgcagggcat gtttgccttt gccctgtacg acagcgaaaa agatgcctac ctgattggtc
    29401 gcgaccatct ggggatcatc ccactgtata tggggtatga cgaacacggt cagctgtatg
    29461 tggcctcaga aatgaaatcg ctggtgccag tttgccgcac gattaaagag ttcccggcgg
    29521 ggagctattt gtggagtcag gacggcgaaa tccgttctta ttatcatcgc gactggttcg
    29581 actttgatgc agtgaaagat aacgtgaccg acaaaaacga gctgcgtcag gcactggaag
    29641 attccgttaa aagccatctg atgtctgatg tgccttacgg tgtgctactt tctggtggtc
    29701 tggattcctc aattatttcc gctatcacca agaaatacgc agcccgtcgc gtggaagatc
    29761 aggaacgctc tgaagcctgg tggccgcagt tacactcctt tgctgtaggt ctgccgggtt
    29821 caccggatct taaagcagcc caggaagtgg caaaccatct gggcacggtg catcacgaaa
    29881 ttcacttcac tgtacaggaa ggtctggatg ccatccgcga cgtgatttac cacatcgaaa
    29941 cttatgatgt gaccactatt cgcgcttcaa caccgatgta tttaatgtcg cgtaagatca
    30001 aggcgatggg cattaaaatg gtgctgtccg gtgaaggttc tgatgaagtg tttggcggtt
    30061 atctttactt ccataaagcg cccaacgtta aagaactgca tgaagagacg gtgcgtaaac
    30121 tgctggcctt gcatatgtat gactgcgcgc gcgccaacaa agcgatgtca gcctggggcg
    30181 tggaagcacg cgttccgttc ctcgacaaaa aattccttga tgtggcgatg cgcattaacc
    30241 cgcaggataa aatgtgcggt aacggcaaaa tggaaaaaca catcctgcgt gaatgttttg
    30301 agtcatacct gcccgcaagc gtggcctggc ggcagaaaga gcagttctcc gatggcgtcg
    30361 gttacagttg gatcgacacc ctgaaagaag tggcggcgca gcaggtttct gatcagcaac
    30421 tggaaactgc ccgcttccgc ttcccgtaca acacgccgac ctcaaaagaa gcgtatctgt
    30481 accgcgagat ctttgaagaa ctgttcccac ttccgagtgc cgctgagtgc gtgccgggcg
    30541 gtccttccgt cgcgtgttct tcagctaaag cgatcgaatg ggatgaagcg ttcaaaaaaa
    30601 tggacgatcc gtctggtcgt gcggttggcg ttcaccagtc ggcgtataag taagaaatgc
    30661 ggtactgccc cggagaaatc cggggctgtt ttagttattg cgctgcttgt tgattctgat
    30721 atgcagtaag gaaatgttcc gggtttgcca catcactgtt tgacaactca tcactgtagg
    30781 ccagtatttc atagattaat ttcccaccac tattgttgaa ttccgaattt catcctgtac
    30841 gacaaaagaa tggctgtagg gtggggaaaa acacaaagta aacaataatt gacgaatata
    30901 gcgccacgct gttcgcaacc taaccaaaca gtcactttcg agcaattttc cttgaaaaag
    30961 aggttgacgc tgcaaggctc tatacgcata atgcgccccg caacgccgat aaggtatcgc
    31021 gaaaaaaaga tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
    31081 acaggttcga atcccgtcgt agccaccatc ttttttgcgg gagtggcgaa attggtagac
    31141 gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc
    31201 attcaccaga aagcgttgta cggatggggt atcgccaagc ggtaaggcac cggtttttga
    31261 taccggcatt ccctggttcg aatccaggta ccccagccat cttcttcgag taagcggttc
    31321 accgcccggt tattggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc
    31381 cctggttcga atccaggtac cccagccatc gaagaaataa tctggctacg tagctcagtt
    31441 ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca
    31501 aattctgaat gtgtcgaata cgttcggcaa at
//
LOCUS       DAJTYH010000040        31123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTYH010000040 DAJTYH010000000
VERSION     DAJTYH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1226
                     /gene="waaA"
                     /locus_tag="OO609_001829"
     CDS             <1..1226
                     /gene="waaA"
                     /locus_tag="OO609_001829"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6633268.1"
                     /translation="IRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIMLHSVSVGETL
                     AAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHVYLPYDLPDALNRFLNK
                     VDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGYAKLGKFVRRLLRRIT
                     LIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRRQWAPHRPVWI
                     ATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQAGLSYITRSSG
                     EVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAHAIPVLMGPHT
                     FNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRSFYGRHAVEVLYQNQGALQ
                     RLLQLLEPYLPPKTH"
     gene            1234..1713
                     /gene="coaD"
                     /locus_tag="OO609_001830"
     CDS             1234..1713
                     /gene="coaD"
                     /locus_tag="OO609_001830"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6633269.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1752..2561)
                     /gene="mutM"
                     /locus_tag="OO609_001831"
     CDS             complement(1752..2561)
                     /gene="mutM"
                     /locus_tag="OO609_001831"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6633270.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2659..2826)
                     /gene="rpmG"
                     /locus_tag="OO609_001832"
     CDS             complement(2659..2826)
                     /gene="rpmG"
                     /locus_tag="OO609_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6633271.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2847..3083)
                     /gene="rpmB"
                     /locus_tag="OO609_001833"
     CDS             complement(2847..3083)
                     /gene="rpmB"
                     /locus_tag="OO609_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6633272.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3300..3968)
                     /gene="radC"
                     /locus_tag="OO609_001834"
     CDS             complement(3300..3968)
                     /gene="radC"
                     /locus_tag="OO609_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6633273.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4140..5360
                     /gene="coaBC"
                     /locus_tag="OO609_001835"
     CDS             4140..5360
                     /gene="coaBC"
                     /locus_tag="OO609_001835"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6633274.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5338..5796
                     /gene="dut"
                     /locus_tag="OO609_001836"
     CDS             5338..5796
                     /gene="dut"
                     /locus_tag="OO609_001836"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6633275.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            5903..6499
                     /gene="slmA"
                     /locus_tag="OO609_001837"
     CDS             5903..6499
                     /gene="slmA"
                     /locus_tag="OO609_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6633276.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6536..7177)
                     /gene="pyrE"
                     /locus_tag="OO609_001838"
     CDS             complement(6536..7177)
                     /gene="pyrE"
                     /locus_tag="OO609_001838"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6633277.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7243..7959)
                     /gene="rph"
                     /locus_tag="OO609_001839"
     CDS             complement(7243..7959)
                     /gene="rph"
                     /locus_tag="OO609_001839"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6633278.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8086..8949
                     /locus_tag="OO609_001840"
     CDS             8086..8949
                     /locus_tag="OO609_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6633279.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9170..9994
                     /gene="dinD"
                     /locus_tag="OO609_001841"
     CDS             9170..9994
                     /gene="dinD"
                     /locus_tag="OO609_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6633280.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10285..10902
                     /locus_tag="OO609_001842"
     CDS             10285..10902
                     /locus_tag="OO609_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6633281.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10899..12581)
                     /gene="ligB"
                     /locus_tag="OO609_001843"
     CDS             complement(10899..12581)
                     /gene="ligB"
                     /locus_tag="OO609_001843"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6633282.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12839..13462
                     /gene="gmk"
                     /locus_tag="OO609_001844"
     CDS             12839..13462
                     /gene="gmk"
                     /locus_tag="OO609_001844"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6633283.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13517..13792
                     /gene="rpoZ"
                     /locus_tag="OO609_001845"
     CDS             13517..13792
                     /gene="rpoZ"
                     /locus_tag="OO609_001845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6633284.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13811..15919
                     /gene="spoT"
                     /locus_tag="OO609_001846"
     CDS             13811..15919
                     /gene="spoT"
                     /locus_tag="OO609_001846"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6633285.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            15926..16615
                     /gene="trmH"
                     /locus_tag="OO609_001847"
     CDS             15926..16615
                     /gene="trmH"
                     /locus_tag="OO609_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6633286.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16621..18702
                     /gene="recG"
                     /locus_tag="OO609_001848"
     CDS             16621..18702
                     /gene="recG"
                     /locus_tag="OO609_001848"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6633287.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18736..19941)
                     /gene="gltS"
                     /locus_tag="OO609_001849"
     CDS             complement(18736..19941)
                     /gene="gltS"
                     /locus_tag="OO609_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6633288.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20221..21612
                     /gene="xanP"
                     /locus_tag="OO609_001850"
     CDS             20221..21612
                     /gene="xanP"
                     /locus_tag="OO609_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6633289.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21733..23442
                     /locus_tag="OO609_001851"
     CDS             21733..23442
                     /locus_tag="OO609_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6633290.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23495..25813)
                     /gene="yicI"
                     /locus_tag="OO609_001852"
     CDS             complement(23495..25813)
                     /gene="yicI"
                     /locus_tag="OO609_001852"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6633291.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25823..27206)
                     /locus_tag="OO609_001853"
                     /pseudo
     CDS             complement(25823..27206)
                     /locus_tag="OO609_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27499..27593
                     /locus_tag="OO609_001854"
     tRNA            27499..27593
                     /locus_tag="OO609_001854"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27533..27535,aa:Sec,seq:tca)
     gene            27865..29076
                     /locus_tag="OO609_001855"
     CDS             27865..29076
                     /locus_tag="OO609_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6633292.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29488..30531
                     /locus_tag="OO609_001856"
     CDS             29488..30531
                     /locus_tag="OO609_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6633293.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT         7784 a         8211 c         7733 g         7395 t
ORIGIN      
        1 ggatacggct ctgggtgcgc ggacgtaagg ctccggccta tcgaaaacgc tggggtgaac
       61 gttacggttt ttaccgccat ccgctaaaac caggcggcat tatgctgcac tccgtctccg
      121 tcggtgaaac tctggcggcg atcccgttgg tgcgcgcgct gcgtcatcgt tatcctgatt
      181 taccgattac cgttacaacc atgacgccaa ccggttcgga gcgcgtacaa tcggctttcg
      241 ggaaggatgt tcagcacgtt tatctgccgt atgacctgcc cgatgcgctc aaccgtttcc
      301 tgaataaagt cgaccccaaa ctggtgttga ttatggaaac cgaactatgg cctaacctga
      361 ttgcggcgct acataaacgt aaaattccgc tggtgatcgc taacgcgcga ctctctgccc
      421 gctcggccgc aggttatgcc aaactgggta aattcgtccg ccgcttgctg cgtcgtatta
      481 cgctgattgc tgcgcaaaat gaagaagatg gtgcacgttt tgtggcgctg ggcgcaaaaa
      541 ataaccaggt aaccgttacc ggtagcctga aattcgatat ttctgtaact ccgcagttgg
      601 ctgctaaagc cgtgacgcta cgccgccaat gggcaccaca ccgcccggta tggattgcca
      661 ccagcactca cgaaggtgaa gagagcgtgg tcattgccgc acatcaggca ttgttacagc
      721 aattcccgaa tttattgctc atcctggtac cccgtcatcc agaacgcttc ccggatgcga
      781 ttaaccttgt ccgccaggct ggactaagct atatcacacg ctcttcaggg gaagtcccct
      841 ccaccagcac gcaggttgtg gttggcgata cgatgggcga gttgatgtta ctgtacggca
      901 ttgccgatct cgcctttgtt ggcggttcac tggttgaacg tggtgggcat aatccgctgg
      961 aagctgccgc acacgctatt ccggtattga tggggccgca tacttttaac tttaaagaca
     1021 tttgcgcgcg gctggagcag gcaagcgggc ttattaccgt taccgatgcc actacgcttg
     1081 caaaagaggt ttcctcttta ctcaccgacg ccgtttaccg tagtttctat ggccgtcatg
     1141 ccgttgaagt actgtatcaa aaccagggcg cgctacagcg tctgcttcaa ctgctggaac
     1201 cttacctgcc accgaaaacg cattgaggtt gttatgcaaa aacgggcgat ttatccgggt
     1261 actttcgatc ccattaccaa tggtcatatc gatatcgtga cgcgcgccac gcagatgttc
     1321 gatcacgtta ttctggcgat tgccgccagc cccagtaaaa aaccgatgtt taccctggaa
     1381 gagcgtgtgg cactggcaca gcaggcaacc gcgcatctgg ggaacgtgga agtggtcggg
     1441 tttagtgatt tgatggcgaa cttcgcccgt aatcaacacg ctacggtgct gattcgtggc
     1501 ctgcgtgcgg tggcggattt tgaatatgaa atgcagctgg cgcatatgaa tcgccactta
     1561 atgccggaac tggaaagtgt gtttctgatg ccgtcgaaag agtggtcgtt tatctcttca
     1621 tcgttggtga aagaggtggc gcgccatcag ggcgatgtca cccatttcct gccggagaat
     1681 gtccatcagg cgctgatggc aaaattagcg tagcgtttat gccggatggt atgccatccg
     1741 gctcgcatga attacttctg gcactgccga caataaaacg ttgcccgctg cgcatgttta
     1801 gtcgccacaa tcggcgtacc gcacacccga cacggctcac cttttcgccc gtaaacctgc
     1861 aattcctgcg cgaaatagcc cggtttacca tcactttgca gaaaatcttt cagcgttgta
     1921 ccaccctgct caatcgaacg cagcaacacc gctttaatca cccgagctaa caattcacac
     1981 tctgccagcg acagtgatga cgccagccga tccggatgga tccccgccgc aaacagtgat
     2041 tcgctggcat agatattccc tacccctacc accagcttgt tatccatcag ccacggttta
     2101 atcgccgttt ttttcttcgc gcacttctga tgcagatact caccattgaa atcgtcgcta
     2161 agcggctccg gtccaagatg ggccagcaca ttatgccctt ccagctcttt ggtccacagc
     2221 caggcaccaa agcggcgcgg atcggtgtag cgcagcactt taccgttgct catcaccaaa
     2281 tccacatggt catgcttttc agggggaagt tcttctggaa ggatgcgcag gctgccagac
     2341 atccccaaat gaatgataat ccagccctca ggcagctcca gcagcagata tttagcccgc
     2401 cgctgcacgc taagcactgg ttgatcgctt aatcggtaga tctcttctga aaccggccag
     2461 cgcaagcgtc cgttgcgcac caccgcatga agaatggttg caccaacgag atgcggttct
     2521 atgccgcgac ggctggtttc aacttcgggt aattcaggca tagcatctcc aggaatgaac
     2581 agatgcaatc aatatggggg caagcagata acaaaaaacc ccgccggagc gaggtttttc
     2641 gttacatcaa agcgagaatt atttgatttt cgcttctttg tagatcacgt gctgacgaac
     2701 aactggatcg aattttttca gttccagttt ttccggctta gtacgtttgt tcttcgtagt
     2761 ggtatagaag tgaccagtac cagcagaaga aaccagcttg attttctcac gaataccttt
     2821 agccatgatt tatttcctct aagtacttag tacttttcgc cacgggcacg cagttcagcc
     2881 agaactgtat cgatgccttt tttatcgatt acacgcatac ctttagcaga tacgcgcagg
     2941 gtgacaaaac gcttctcgct ctcaacccag aaacggtgag agtgcaggtt cggcaggaaa
     3001 cggcgtttag tcgcgttcag tgcgtgggaa cggttgttac cggtcaccgg acgcttgcca
     3061 gtaacttggc agactcggga catgtctatt ctccaaaaat caaattagct cgagcttcgt
     3121 atggggtatt ggcgcctcgt caggctttac agcccggtca tcgcagttct atgtgaactc
     3181 tcgattgcca ggcccaaatg ccaaacccga gattctcaaa ggtggcgtag tatacgctga
     3241 ctcagcgatg tgctcaagtc ccgaacagac aaagatcccg aaggatcgcg catagcgggt
     3301 taaatccagc cgcgttcggc aaaagaaaca tactctccac gcccaatcac gatatggtcg
     3361 agcacgcgta aatccatgaa ctgacaactc tttattatcc gttcagtaat gagtttatcc
     3421 gctttactgg gttcagcaca acccgaaggg tgattatgtg caaggatcag cgccgaggcg
     3481 tttattttta tcgcttcgcg gataatttcc cgaggatgga cttcaacatg gtttagcgtg
     3541 ccggaaaaaa gacggctatg cgttataacc cggtgttgtg agtcgagaaa gatcaccata
     3601 aagatctccc gctcctcacc cgtgagctgg ctttgtaaaa attcccgcgt catctccggg
     3661 ctgagtaaag ggctttcttc acgcatccgc acgttgtagt aacgtcgcgc cagttcagca
     3721 atccccttta actgggcaaa tttcgctacg ccaattccat gaacaccact aaattgttca
     3781 tattcagagg ttaacaagcc ataaagagag ccgaaattct ccagcatctc ttttgccagg
     3841 gttaatacat ctttaccgcg cgttccggta cgcagaaata gcgccagcag ctcgacatcc
     3901 gttaaggcgc taataccaaa cttcagcatt ttttcgcgcg gcatcaacag ctgtgcattg
     3961 tttttcacct tcacctcctt tgtggtgctc gcatcctgtc atagccgttc cgttaaatcg
     4021 acggccagtt ttcaatcctg gaaagagcct cgcaaagtga atctcaggtg aaggcacgac
     4081 aacaaaagta ttgtgataaa atcgccaact tctggtgtca cccaacagga aaaatcatca
     4141 tgagcctggc cggtaaaaaa atcgttctcg gcgttagcgg cggtattgct gcctataaaa
     4201 cccctgaact ggtgcgtcgt ttgcgcgatc gcggggccga cgtccgcgta gccatgaccg
     4261 aagcggcaaa agcctttatc accccactta gcttgcaggc ggtttctggt tatcccgttt
     4321 ccgacagtct gcttgacccg gcagccgaag ccgctatggg ccatattgag ctgggtaaat
     4381 gggctgattt agtgattctc gcccctgcca cggcagattt gattgcccgt gttgctgccg
     4441 gaatggcgaa tgacctggta tcgacgattt gtctggctac acctgcgcct gtagccgtgc
     4501 tccccgccat gaaccagcag atgtaccgtg ccgctgccac gcagcataat ttagaggtgc
     4561 ttgcttcccg tggtttgctc atctgggggc cagacagtgg cagtcaggct tgtggtgata
     4621 tcggtcctgg acgaatgctc gatccgttaa ccattgtgga tatggcggtg gcgcattttt
     4681 cgcccgtcaa cgacctgaaa catctgaaca ttatgattac cgccggtccg acgcgtgaac
     4741 cgctcgatcc ggtgcgttat atctctaatc acagctccgg caagatgggt tttgctatcg
     4801 ccgccgctgc tgcccgtcgt ggcgcgaacg tcacgctggt atcaggtcca gtttcactac
     4861 cgacgccacc gtttgttaaa cgtgttgatg tgatgaccgc gctggaaatg gaagccgccg
     4921 tgaatgcttc tgtacagcag caaaatattt ttatcggttg cgccgccgtg gcggattatc
     4981 gcgcagctac cgtggcccca gaaaaaatca aaaagcaggc cacgcagggt gatgaattaa
     5041 caataaaaat ggttaaaaac cccgatatcg tcgcaggcgt tgccgcacta aaagaccatc
     5101 gaccctacgt cgttggattt gccgccgaaa caaataatgt ggaagaatac gcccggcaaa
     5161 aacgtatccg taaaaacctt gatctgatct gcgcgaacga tgtttcccag ccaactcaag
     5221 gatttaacag cgacaacaac gcattacacc ttttctggca ggacggagat aaagtcttac
     5281 cgcttgagcg caaagagctc cttggccaat tattactcga cgagatcgtg acccgttatg
     5341 atgaaaaaaa tcgacgttaa gattctggac ccgcgcgtta ggaaggaatt tccgctcccg
     5401 acttatgcca cctctggctc tgccggactt gacctgcgtg cctgtctcga cgacgccgta
     5461 gaactggctc cgggtgacac tacgctggtt ccgaccgggc tggcgattca tattgccgat
     5521 ccttcactgg cggcaatgat gctgccgcgc tccggattgg gacataagca cggtatcgtg
     5581 cttggtaacc tggtaggatt gatcgattcc gactatcagg gccagttgat gatttccgtg
     5641 tggaaccgtg gtcaggacag cttcaccatt caacctggcg aacgcatcgc ccagatgatt
     5701 tttgttccgg tagtacaggc tgaatttaat ctggtggaag atttcgacgc caccgaccgc
     5761 ggtgaaggcg gctttggtca ctctggtcgt cagtaacaca tacgcatccg aataacgtca
     5821 taacatagcc gcaaacattt cgtttgcggt catagcgtgg gtgccgcctg gcaagtgctt
     5881 attttcaggg gtattttgta acatggcaga aaaacaaact gcgaaaagga accgtcgcga
     5941 ggaaatactt cagtctctgg cgctgatgct ggaatccagc gatggaagcc aacgtatcac
     6001 gacggcaaaa ctggccgcct ctgtcggcgt ttccgaagcg gcactgtatc gccacttccc
     6061 cagtaagacc cgcatgttcg atagcctgat tgagtttatc gaagatagcc tgattactcg
     6121 catcaacctg attctgaaag atgagaaaga caccacagcg cgcctgcgtc tgattgtgtt
     6181 gctgcttctc ggttttggtg agcgtaatcc tggcctgacc cgcatcctca ctggtcatgc
     6241 gctaatgttt gaacaggatc gcctgcaagg gcgcatcaac cagctgttcg agcgtattga
     6301 agcgcagctg cgccaggtat tgcgtgaaaa gagaatgcgt gagggtgaag gttacaccac
     6361 cgatgaaacc ctgctggcaa gccagatcct ggccttctgt gaaggtatgc tgtcacgttt
     6421 tgtccgcagc gaatttaaat accgcccgac ggatgatttt gacgcccgct ggccgctaat
     6481 tgccgcgcag ttgcagtaat atgacgccgg atgacttttc atccggcgag tttctttaaa
     6541 cgccaaactc ttcgcgatag gccttaaccg ccgccagatg ttccgccatt tccggcttct
     6601 cttccagata ggcaatcaga tctttcaggg tgatgataga gatcactttg cagttgtaat
     6661 cacgctcaac ttcctgaatc gccgaaatct cgccgcgccc gcgttcctga cgatcgagcg
     6721 aaatcaacac gccagcaagc gtcgcgccat tggcctgaat aatctccatc gactcgcgaa
     6781 tcgccgttcc ggcggtgatc acatcatcta ccagcattac gcgtccttgt aacgcgctac
     6841 caaccagatt gccgccttca ccgtggtctt ttgcttcttt gcggttaaag cagtacggca
     6901 ggtccaggtc gtgatgctcc gccagtgcca cagcggttgt ggtggcaatc gggatccctt
     6961 tgtaagcagg gccaaacagc agatcgaact caatgccgga atccaccaac gcttcagcgt
     7021 aaaaacggcc taacagtgcc agatcgcgcc cggtattaaa cagcccggcg ttgaagaaat
     7081 aggggctttt gcgcccggat ttcagcgtaa actcgccaaa ctttaacacc tgcttgctaa
     7141 gcgcaaattc aataaactgg cgctgatatg atttcatgcc ttcgctcctc atcttacttt
     7201 tctacagaca aaaaaaaggc gactcatcag tcgccttaaa aatcagtttg ccagcgccgc
     7261 cttctgcgtc gctacaatgg attcgattcc ccctcgggcc agcgccaaca aggtgagtag
     7321 ctcttcatgg gtgaacggct cgccttctgc cgtcccctgc acttcaatga tgcgcccgtc
     7381 ttcggtcatc actacgttca tgtcggtctc tgcggctgag tcttcaacgt attccagatc
     7441 gcaaattgct tcaccgttta caataccgac agaaactgcg gctaccatcc ctttcatcgg
     7501 gttggttttc agcttgccgt tttccaccag cttctgtagc gcatctgcca gcgccacgca
     7561 ggcacccgta atcgacgcgg tacgagtgcc accatcagcc tgaagcacgt cgcagtccag
     7621 cgtaatggtg aactcaccca gcgctttcaa atctactgcc gcgcgaagag cacgggcgat
     7681 cagacgctgg atttccattg tgcgtccacc ctgcttacct ttcgccgctt cacgagcgtt
     7741 acgggtgtgg gtagaacgtg gcagcatgcc gtactctgcg gtgatccagc cctggccctg
     7801 acctttcagg aagcgcggca cgccttcttc aatagaggcg gtacacaata ctttggtatc
     7861 gccaaattcg accagcaccg agccttctgc atgttttgta tagttacgag tcagggtaac
     7921 gggacgcacc tgattattgc tacggcctgc tggacgcata ttgaaatctc cggcttgaaa
     7981 caaatgtggc tgcgcattat acggacttcc ggcggttatt cctatcctga caaggcatcg
     8041 atggctataa tccttccacc tctcctttta taaacaggaa cgtctatgat ccgcagtatg
     8101 accgcctacg cccggcgtga aatcaagggt gaatggggga gcgcaacctg ggaaatgcgc
     8161 tcggtaaacc agcgttatct ggaaacttac tttcgtctgc cggagcagtt ccgtagcctt
     8221 gaacctgtcg ttcgcgagcg tattcgttct cgcctgacgc gcggtaaagt ggaatgtacc
     8281 ctgcgctatg agccagatgt tagcgcacaa ggtgagctga tcctcaatga aaaactggct
     8341 aaacagctgg taactgccgc gaactgggta aaaatgaaga gtgacgaagg ggaaatcaac
     8401 ccggttgata ttctacgctg gccgggcgtg atggcagccc aggagcagga tctcgacgcg
     8461 attgccgctg aaattctcgc ggcgttggat ggtacgctgg acgactttat cgtcgcgcgc
     8521 gaaaccgaag gtcaggcact gaaagcattg atcgagcagc gtctggaagg cgtcaccgcc
     8581 gaagtggtca aagtccgcgc ccatatgccg gaaattctgc aatggcagcg tgagcgtctg
     8641 gtcgcgaagc tggaagatgc tcaggttcag ttggaaaaca accgtctgga gcaggagttg
     8701 gttctgctgg cacaacgaat tgacgttgcc gaagagctgg accgcctcga agcgcatgtt
     8761 aaagagacgt acaacattct gaagaaaaaa gaagcggttg gtcgtcgtct ggactttatg
     8821 atgcaggagt tcaaccgtga gtcgaacact cttgcgtcga aatctatcaa tgccgaagtg
     8881 acaaactccg ccatcgagct gaaagtgttg attgagcaga tgcgcgagca gattcagaat
     8941 attgagtaac tcgtaacacc aattctgact tttcagcctt cacaaatgcc gcccactcaa
     9001 acagagcggc atttttcttc cccgcaactt caattctgtt ttgcgtgcct gctccagatt
     9061 ttgcgatgtt tttttgccca gcacactgag aacgtgagat actcacaact gtatataaat
     9121 acagttacag atttactttc tttgcaatcg atatcacatg gagtgggcaa tgaacgaaca
     9181 tcatcaacct tttgaagaga taaaactgat taatgcaaac ggagcagaac aatggtcagc
     9241 aagacaactt gggaaactac tgggttattc agagtatcgt cactttatac ctgtattaac
     9301 gcgcgccaaa gaggcctgtg agaacagtgg tcacacaatt gatgaccatt tcgaggagat
     9361 cctcgatatg gtcaaaattg gctcaaatgc caaaagagca ttaaaagaca tcgtactctc
     9421 ccgctatgcc tgttacctgg tagtacaaaa cggcgacccc gcgaaaccgg tcattgcggc
     9481 agggcagact tattttacta tccagacccg acggcaggag cttgctgacg atgaagcatt
     9541 taagcaactt cgtgaagacg aaaagcgtct gtttctgaga aatgagttga aagaacataa
     9601 caaacaattg gttgaggcag cacagcaagc tggggtagcg acagctactg atttcgccat
     9661 atttcagaat catggttacc aggggctgta tggtggatta gatcagaaag ccatccacca
     9721 actgaagggg ctgaaaaaga gtcagaagat cctggatcat atgggttcaa cagaactggc
     9781 ggctaatctc tttcgagcta cccaaacaga agaaaaactc aagcgggata gcgtgaattc
     9841 aaaacagcaa gctaatacca cacactttga cgtgggtagc aaggtgaggc aaaccattca
     9901 ggaacttggc ggaaccatac ctgaggagtt gccgaccccg caggtcagca tcaagcagtt
     9961 ggaaaacagt gtaaaaatta cagagaagaa atagcagaaa cgaaaaactc tttttgttga
    10021 ttgagacacc cgatgcgtaa gccaaggtcc aggtgcagtg aacacaatgg ctaaacattg
    10081 cacctttctt ttcccccctc agttttaacc tattttttct tatgcatttt ctcagacaag
    10141 aagtcagaag aatgcatcta cgctacagaa aatagcgatt tcacataact aaaagttatc
    10201 aatttcctct caccttaaaa aatatcaatc gtgacaatgc gcacaaatcg ctaccctgcc
    10261 agacagattt ttagggagag aaccatgctg ttacacattt tgtatttggt tggcattact
    10321 gccgaagcca tgacgggggc gctggcggcc gggcgacggc gcatggatac atttggcgta
    10381 attattattg caaccgccac cgcaattggc ggagggtcag tgcgcgatat tctgctgggc
    10441 cactatccgc tcggctgggt caaacacccg gaatatgtga ttatcgtcgc taccgccgca
    10501 gtactcacca ctatcgttgc tcctgtaatg ccttacctgc gcaaagtgtt tctggtgctc
    10561 gatgcgctcg gactggtggt cttttctatc atcggcgcac aggttgcact ggatatgggg
    10621 cacggcccaa ttattgccgt tgtcgcggcg gtaaccaccg gtgtatttgg cggcgttttg
    10681 cgcgatatgt tctgtaaacg cattccgctg gtctttcaaa aagagttgta tgccggtgtc
    10741 tcatttgcct ccgccgtgct gtacatcgcg ctgcaacact atgtttctaa ccatgatgtg
    10801 gtgattatct ccaccctggt attcggcttt tttgctcgtt tactggcgct acgtcttaag
    10861 ctgggattac cggtctttta ctacagccac gaaggtcact aaggttcaaa acctgtgatc
    10921 tgctgggcag ccagccaact gcccagcttc ttgatttgcg cattttcctt ccattcaata
    10981 acctgtctgg cgcgcccaga tccagtcccc ggcagctgct gccagaactg ctccgtgcta
    11041 aataaaagtt gcgaccagga ccgttcatca ctggcattaa gcgccgcccg ggttagcggt
    11101 attcccattg ccatcaccca gcgagtaaaa ggctggttac gagccagatt aaactgatgc
    11161 cataactgcg cacttttact tttcgcgatc cccggcgtgt tctgtaattg ctctggcgtt
    11221 aataaaagcc aggaaaagat atgctcaaag cgatgagtct gatgcagcgc gcgccaaccg
    11281 gcctcaccaa tgccatccag ccccagaacc tgttttgacc ccagccagac taagcgtgaa
    11341 atgaactgtt cctgacaaac atcagaagca aagtagcagg tcaacgggtt aaagcggttt
    11401 tctggtggtg tcggttttgt acgttctgca ccgcgccaga ccacatcatc aatgcgagga
    11461 ataccctgac cggcaaggct gacgaggatc tgatcaccag gcgcaatatc ccactcctgc
    11521 cagcgcctga cggaaccaat attcacccgc tggacttttt tatcatccag catgacaggt
    11581 gcgagtgacg caaccaccga tattttaccg ctcttaccca ccgcaaactg aatcgccttc
    11641 acttcggcaa cctgagctac aggttgatat ttccaggcca ccagccactc cgcctggccc
    11701 ggtagccaat gacgggattc gggttcctta gcccctcgca caaccacgcc atcggtgacg
    11761 aagggtaatt tcgctttcca ccactcattg cgtacgcgcg caacctcatc agcattttta
    11821 accgcacggg tatacctctg cgttagagta aaacctgcgg tagccagctc ctttaaacga
    11881 tcggtcatta actgcggtcc atccggccat gcccagacaa aaacagccag tgagtttagc
    11941 gtgtcgctat tcccctggcg catcatcagg ccagcaactt ttgcgcgggc atttattccc
    12001 cccatttgtt gttggatatg cccctcgcgc tggagaaata tttccccctg aagcgtactg
    12061 ttggctaaag gcccgctaac ggtttgcagc acagcggaaa ttaagctaac tttctgcgtc
    12121 cagtcctcgc ctttcaggcc attaccgcga ctgattgctt tgttcagttt cccgtcccga
    12181 taaaccaggg ttaccgcaac gccatcaact tttggctgca cccaaagatc gctacgttct
    12241 cgcatccaca gacttaatgc gtttttatcc gccattttgc gtacacccgt atgggcaacc
    12301 ggatgcataa ccgcaccatt taaaggcggc atcatcacgt cgcggggctc gctcacaaag
    12361 cagcgttgcc actgcgtaag acgggcgctt aactgatcgt aaacaccgtc ttccacctca
    12421 ctttttcctt ccttccagta gtcatcgtcc cactgtttta tttgctgttg caggcgggaa
    12481 atttcttcct gtgctctggc tggcgaccag gccggacaga ccgcccacac agatgattgc
    12541 cagcacaaga tacttattaa tatcgccatc catactttca tcttcacctc cgctgtagtc
    12601 aggctggcag atatacaacg tggtgaaggc aaagccgagt ggcaaaaacg gagtctgcga
    12661 ggacgcttcc tgagaatcgt ctttattgca gtaaatcaca ggcaaatgcg gaaacagcta
    12721 cgcaaaacgc aacaactttg cgcaaaaagt gtgagcaagg gctacgtcac atggccgcgc
    12781 cgtgtataat aagctcgtat gtaggcttta tttcgctaat cacatacgaa agatactcat
    12841 ggctcaaggc acgctttata ttgtttctgc ccccagtggc gcgggtaaat ccagcctgat
    12901 tcaggcttta ttaaaaaccc aaccgttgta tgacacccag gtttctgttt cacacaccac
    12961 acgccaaccg cgtcctggtg aagtccacgg tgaacattat ttctttgtta atcatgatga
    13021 atttaaagaa atgattagca gagatgcgtt cctcgaacac gcagaagttt ttggtaatta
    13081 ctatggcact tcgcgtgagg ccattgagca agtactggcg accggtgtcg atgtttttct
    13141 cgatatcgac tggcagggcg cgcagcaaat tcgccagaag atgccgcacg cgcggagtat
    13201 ctttatttta ccgccgtcca aaattgaact ggaccgccgt ctacgcggtc gcggtcagga
    13261 cagcgaagag gtcattgcaa agcgtatggc gcaagctgtt gcagaaatga gccattacgc
    13321 cgaatatgat tatctgattg tgaatgatga cttcgatacc gcgttgaccg atttgaagac
    13381 cattattcgc gccgaacgtc tgcgcatgag ccgccaaaag cagcgtcatg acgctttaat
    13441 cagcaaattg ttggcagact gaacctgatt tcagtatcat gcccagtcat ttcttcacct
    13501 gtggagcttt ttaagtatgg cacgcgtaac tgttcaggac gctgtagaga aaattggtaa
    13561 ccgttttgac ctggtactgg tcgccgcgcg tcgcgctcgt cagatgcagg taggcggaaa
    13621 ggatccgctg gtaccggaag aaaacgataa aaccactgta atcgcgctgc gcgaaatcga
    13681 agaaggtctg atcaacaacc agatcctcga cgttcgcgaa cgccaggaac agcaagagct
    13741 ggaagccgct gaattacaag ccgttaccgc tattgctgaa ggtcgtcgtt aatcacaaag
    13801 cgggtcgccc ttgtatctgt ttgaaagcct gaatcaactg attcaaacct acctgccgga
    13861 agaccaaatc aagcgtctgc ggcaggcgta tctcgttgca cgtgatgctc acgaggggca
    13921 aacacgttca agcggtgaac cctatatcac gcacccggta gcggttgcct gcattctggc
    13981 cgagatgaaa ctcgactatg aaacgctgat ggcggcgctg ctgcatgacg tgattgaaga
    14041 tactcccgcc acctaccagg atatggaaca gctttttggt aaaagcgtcg ccgagctggt
    14101 agagggggtg tcgaaacttg ataaactcaa gttccgcgat aagaaagagg cgcaggccga
    14161 aaactttcgc aagatgatta tggcgatggt gcaggatatc cgcgtcatcc tcatcaaact
    14221 tgccgaccgt acccacaaca tgcgcacgct gggctcactt cgcccggaca aacgtcgccg
    14281 catcgcccgt gaaactctcg aaatttacag cccgctggcg caccgtttag gtatccacca
    14341 cattaaaacc gaactcgaag agctgggttt tgaggcgctg tatcccaacc gttatcgcgt
    14401 aatcaaagaa gtggtgaaag ccgcgcgcgg caaccgtaaa gagatgatcc aaaaaatcct
    14461 ctcggaaatc gaagggcgtt tgcaggaagc gggaattccc tgccgcgtca gtggtcgcga
    14521 gaagcatctt tattcgattt actgcaaaat ggtgctcaaa gagcagcgtt ttcactcaat
    14581 catggacatc tacgctttcc gcgtgatcgt caatgattcc gacacctgtt atcgcgtgct
    14641 cgggcaaatg catagcctgt acaagccgcg tccgggtcgc gtgaaagact acatcgccat
    14701 tccaaaagcg aacggctatc agtctttgca cacctcgatg atcggcccgc acggtgtgcc
    14761 ggttgaggtc cagatccgta ccgaagatat ggatcagatg gcggagatgg gtgttgccgc
    14821 gcactgggct tataaagagc acggcgaaac cagcaccacc gcgcaaatcc gcgcccagcg
    14881 ctggatgcaa agcctgctgg agctgcaaca gagcgccggt agttcgtttg aatttatcga
    14941 gagcgttaaa tccgatctct tcccggatga gatttacgtt ttcacaccgg aagggcgaat
    15001 tgtcgagctg cctgccggtg caacgcccgt cgacttcgct tatgcagtgc ataccgatat
    15061 cggtcatgcc tgcgtgggcg cacgcgttga ccgccagcct tacccgctgt cgcagccgct
    15121 taccagcggt caaaccgttg aaatcattac cgcaccgggc gctcgcccga atgccgcgtg
    15181 gctgaacttt gtcgttagct caaaagcgcg cgccaaaatt cgtcagttgc tgaaaaacct
    15241 caagcgtgat gattctgtaa gcctgggccg tcgtctgctc aaccatgctt tgggtggtag
    15301 ccgtaagctg aatgaaatcc cgcaggaaaa tattcagcgc gagctggatc gcatgaagct
    15361 ggcaacgctt gacgatctgc tggcagaaat cggacttggt aacgcaatga gcgtggtggt
    15421 cgcgaaaaat ctgcaacatg gggacgcctc cattccaccg gcaacccaaa gccacggaca
    15481 tctgcccatt aaaggtgccg atggcgtact gatcaccttt gcgaaatgct gccgccctat
    15541 tcctggcgac ccgattatcg cccacgtcag ccccggtaaa ggtctggtga tccaccatga
    15601 atcctgccgt aatatccgtg gctaccagaa agagccagag aagtttatgg ctgtggaatg
    15661 ggataaagag acggcgcagg agttcatcac cgaaatcaag gtggagatgt tcaatcatca
    15721 gggcgcgctg gcaaacctga cggcggcaat taacaccacg acttcgaata ttcaaagttt
    15781 gaatacggaa gagaaagatg gtcgcgtcta cagcgccttt attcgtctga ccgctcgtga
    15841 ccgtgtgcat ctggcgaata tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt
    15901 cacccgaaac cgaaattaat gttttatgaa cccaacacgt tatgcacgca tctgcgaaat
    15961 gctcgccagg cggcagcctg atctgaccgt ctgcatggag caggtccaca aacctcataa
    16021 cgtttctgcg attattcgta ccgcagatgc cgttggcgta catgaagttc acgccgtctg
    16081 gcctggtagc cgcatgcgca ccatggcttc ggcagcggcg ggtagtaaca gctgggtaca
    16141 ggtgaaaaca caccgcacca ttggcgatgc cgtcgctcat ctcaaaggcc agggcatgca
    16201 gattctggca acccatcttt ctgataatgc tgtcgatttc cgcgaaattg attacactcg
    16261 cccgacctgc attttgatgg gacaggagaa aacgggcatc acgcaggaag cattggccct
    16321 ggcggatcag gacatcatca ttccgatgat cggcatggtg cagtcgctga atgtttccgt
    16381 tgcctcagcc ctcattcttt acgaagccca gcgtcagcgg caaaatgcag gcatgtacct
    16441 gcgtgaaaac agcatgttgc cggaagcaga gcaacaacgc ctgttgtttg aaggcggcta
    16501 tccggtgctg gcgaaagtcg caaaacgcaa aggcctgcct tatccccacg tcaatcagca
    16561 aggcgagatc gaagctgatg ccgactggtg ggctactatg caggctgcag ggtaagtgcc
    16621 atgaaaggtc gcctgttaga tgctgtccca ctcagttccc taacgggcgt tggcgcagca
    16681 cttagtaaca aactggcgaa aatcaacctg cataccgtgc aggatctact cttacacctt
    16741 cccctgcgct acgaagatcg cacccatctc taccccatcg gagaactact gccgggcgtt
    16801 tatgccacgg tggaaggcga agtgctgaac tgcaatatct ccttcggcgg tcggcggatg
    16861 atgacctgcc agatcagcga cggttccggc atcctcacca tgcgcttttt caatttcagc
    16921 gcggcaatga aaaatagcct ggcgacgggc cgccgtgtac tggcttatgg cgaagcaaag
    16981 cgcggtaaat atggtgcgga gatgatccac ccggaatacc gcgtgcaggg cgatctcagc
    17041 acgccagaat tacaggaaac gctcacgccg gtttatccaa caacggaagg cgtaaagcag
    17101 gccacgctgc gtaaattaac cgaccaggcg ctggatctgc tcgacacctg cgccattgaa
    17161 gaactcctgc cgccggaact gtcacaagga atgatgacgc taccggaagc gttgcgcact
    17221 ttgcaccgcc cgccaccgac gctacagctt agcgatctgg aaaccgggca gcatccggcg
    17281 caacgtcgtc tgattctgga agaactgctg gcgcacaacc tcagcatgtt agccttacgt
    17341 gccggagcac agcgttttca tgcccagccg ctgagcgcca atgacacgct gaaaaataaa
    17401 ctcctcgccg ccttaccgtt caagccaacg ggcgcacagg cacgcgtagt ggcggagatc
    17461 gagcatgata tggcgctgga tgtgccgatg atgcgcctgg tacagggcga tgtaggttcc
    17521 ggtaaaacgc tggtcgccgc cctcgccgcg ctgcgtgcga ttgcccacgg caaacaggtg
    17581 gcactgatgg caccaaccga attactcgcc gagcagcacg ccaataactt ccgcaactgg
    17641 tttgcaccgc tcggtatcga agtgggctgg ctcgccggta agcagaaagg caaagcgcga
    17701 ctgtctcagc aggaagccat cgccagcggt caggtgcaga tgattgtcgg tacacacgcc
    17761 atcttccagg aacaggtgca gtttaacggc ctggcgctgg tgattatcga cgaacagcat
    17821 cgttttggcg tgcatcagcg tctggcattg tgggagaaag gccagcagca gggcttccat
    17881 ccgcatcagt tgatcatgac cgccacgccg atcccccgca cgctggcaat gacggcgtat
    17941 gccgatctcg atacctcggt gatagatgag ctgccgccag gccgcacgcc agtgactacg
    18001 gtcgctattc ctgatacccg ccgtaccgac atcattgacc gcgtgcgcca cgcctgcata
    18061 actgaaggtc gtcaggcgta ctgggtttgt acgttgattg aagagtcaga gttactggaa
    18121 gcgcaggcgg cggaagctac ctgggaagag ttgaaactgg cgctaccaga attgaatgtt
    18181 ggcctggtac acgggcggat gaaaccagcc gagaaacagg cggtgatggc gtcgtttaaa
    18241 caaggtgagc tacatctgct ggttgccact accgttattg aagtcggtgt tgatgtgcct
    18301 aacgccagcc tgatgattat cgaaaaccct gagcgtctgg gtctggcgca gttacaccag
    18361 ctgcgcgggc gcgtaggtcg tggcgcggtg gcttctcact gcgtgctgct ctacaaaacg
    18421 ccgctttcta aaacggcgca aattcgcctg caagtgctgc gcgacagtaa cgacggtttt
    18481 gtgattgcgc aaaaagatct ggagattcgc ggccctggcg aattgttagg cacgcgtcag
    18541 acgggtaatg ctgaatttaa agtggcggat ttactgcgcg atcaggcgat gatcccggaa
    18601 gttcagcgcc tggcgcgaca tattcacgaa cgttacccac aacaggcaaa agccttgata
    18661 gaacgctgga tgccggagac agaacgttac tcgaatgcgt aaaaggcggc ggtgagaaga
    18721 ccgccgcttc atcggttaac cggcaaaaat cggcaacatt aaatacagct taatcaccag
    18781 cgcattgacg atatcgataa agaacgcacc gaccatcggc accaccaaaa acgccatgtg
    18841 cgacgggcca aagcgttcag tgatcgcctg catgttggcg attgccgttg gcgttgcacc
    18901 gaggccaaaa ccacagtgac ccgcagccag cactgccgca tcgtagtttt tgcccatcat
    18961 gcgccaggta acgaagatgg catacaacgc catgaagatg gtctgtacca ccagaatcgc
    19021 cagcatcggc agcgccagcg aagccagctc ccacagtttc agccccatca acgccatcgc
    19081 caggaacaac gacaagctta cgttacccag cacggatacc gcacgctcaa agacgcggta
    19141 aaagcccatt attgacagac cgttgctcag aatcacgcca acaaacagta cacagacgaa
    19201 ggtcggcagt tcaaaagcag tgccagccaa aagttgcgca acaattttcc ccaccgtcag
    19261 gcagatagca atcagcgcga tagtttcaat cagcaccaac gaggtgatca tgcgtcccac
    19321 atccggcttt tcaaacgccg tcgggacttc ctggtcatcc ggaataccgt tcggcgtggt
    19381 ggagtgtttc accagatagc gcgccaccgg accgccaatc aagccgccca gcaccagacc
    19441 gaacgttgca caggccatcg ccacttccgt cgcattggtg aagccataac gttcaatgaa
    19501 caatttactc cacgcagcgc ccgtaccgtg accgccggaa agcgtaatag aaccggccaa
    19561 cagccccatc agcggatcaa gccctaacag gctagccata ccaatgccaa tggcattttg
    19621 catcaccaac agaccaacaa ccacaatcaa gaagatgcca accacacgcc caccggcacg
    19681 caaactggca atgttggcgt tcaggccaat ggtggcgaag aaagccagca ttaacggatc
    19741 gcgcagggac atatcaaagt tgacttccca gcccatgctt tttttcagta ctagtagcgc
    19801 cagcgccacc aacaaaccac ccgcaacagg ttccggtatg gtgtatttct tcaaaaagga
    19861 gacggaatgg accaacttac gcccgagcag caacgtcagc gttgcggcaa caagcgttgc
    19921 taaagtatcg agatgaaaca tagttactcc tttgtatccg catcactctt tcatactcgt
    19981 catacttcat cgcgcagtgg caacttcatg aactatgacg ggtatcgagt tatgctttcc
    20041 tgggtcaaat tctcagctgt taatgaacaa atggcatgga ttttaagcag aaaaatacca
    20101 aaagttatat aaaaaagtgc atttatctca tttaaatatt tagatgagga tgtggcaaac
    20161 gtttgctttt ctctttcggt cagctaaaat gcccgctttg ctaccacggg attgttttcg
    20221 atgtctgttt ccaccctcga gtcagaaaat gcgcaaccgg ttgctcagac tcaaaacagc
    20281 gaactgattt accgtcttga agatcgtccg ccgcttcctc aaaccctgtt tgccgcctgt
    20341 cagcatctgc tggcgatgtt cgttgcggtg atcacgccag cgctattaat ctgccaggcg
    20401 ctgggtttac cggcacaaga cacgcaacac attattagta tgtcgctgtt tgcctccggt
    20461 gtggcatcga ttattcaaat taaggcctgg ggtccggttg gctccgggct gttgtctatt
    20521 cagggcacca gcttcaactt tgttgccccg ctgattatgg gcggtaccgc gctgaaaacc
    20581 ggtggtgctg atgttcctac catgatggcg gctttgttcg gcacgttgat gctggcaagt
    20641 tgcaccgaga tggtgatctc ccgcgttctg catctggcgc gccgcattat tacgccgctg
    20701 gtttctggcg ttgtggtgat gattatcggc ctgtcgctaa ttcaggtggg gctgacctcc
    20761 attggcggcg gttacgcagc catgagcgat aacaccttcg gcgcaccgaa aaatctgctg
    20821 ctggcaggcg tggttttagc cttaattatc ctgcttaacc gtcaacgtaa cccttactta
    20881 cgcgtggcct cactggtaat tgcgatggcg gccggatatg cgctggcgtg gtttatgggc
    20941 atgttgccag aaagcaacga accgatgacg caagaactga ttatggtgcc aacgccgctc
    21001 tattacggtc ttggcattga atggagtctg ctgctgccgc tgatgctggt ctttatgatc
    21061 acttcgctgg aaaccattgg cgatatcacg gcgacctctg acgtttccga acagccagtg
    21121 tccggtccgc tgtacatgaa acgcctgaaa ggcggcgtgc tggcaaacgg cctgaactcg
    21181 tttgtttcgg cggtgtttaa caccttcccg aactcctgct tcgggcaaaa caacggagtg
    21241 atccagttga ctggtgttgc cagccgctat gtcggttttg tcgtcgcgct gatgttgatc
    21301 gtgctgggtc tgttcccggc agtgagcggt tttgtacaac acattccaga accggttctg
    21361 ggcggcgcaa cgcttgtaat gtttggcacc atcgccgcct ccggtgtgcg tatcgtttct
    21421 cgtgagccgc tgaaccgtcg ggcgattctg attatcgcgc tgtcgctggc ggttggtctg
    21481 ggcgtgtctc agcagccgct gattttgcag tttgcccctg aatggctgaa aaacctgctc
    21541 tcctccggga tcgccgcggg cggtattact gccatcgtgc tgaatctgat tttcccacca
    21601 gaaaaacagt aattcactgg cggcggtaag ataattaccg ccgcagtctc acctctttca
    21661 ccattcccgc cttgaggatt gcgcgtaaat cgtgcataac tcccttatgt gcatttcacg
    21721 ggatggaaga ccatgaaatt tattgggaag ctgcttctct acattctcat cgctctgtta
    21781 gtggtgatcg ctggcctcta ttttcttctg caaacccgct ggggagcaga acatatcagc
    21841 gcatgggttt ccgagaatag cgactatcat ctggccttcg gggcgatgga tcaccgtttt
    21901 tccgcgccat ctcatatcgt gctggagaat gtcacgtttg gtcgtgatgg tcagcccgcg
    21961 cccctggtgg caaaaagtgt cgacattgcg ctaagcagtc gccaactgac cgaaccacgc
    22021 catgtcgata ccatcctgct ggaaaacggg acgctgaatc tcaccgacca gaccgcgccg
    22081 ctaccgttca aagccgatcg tctgcaactg cgtgatatgg cgtttaatag cccgaatagc
    22141 gaatggaaac tgagcgcgca gcgggtaaat ggcggcgtgg ttccgtggtc accagaagcc
    22201 ggtaaagtgc tgggtacgaa ggcgcagatt cagtttagtg ccggatcgct ttcgctcaat
    22261 gatgttcctg ccaccaatgt actgattgaa ggcagtattg ataacgatcg cgttacgctg
    22321 actaacctgg gtgccgacat cgcccgcggg acattaaccg gaaacgcgca gcgtaacgcc
    22381 gacggcagct ggcaagtgga aaatctgcgc atggcggata tccgtctaca aagcgaaaaa
    22441 tcgctaaccg acttctttgc gccattacgc tctgtcccgt cgttgcagat tggtcgcttg
    22501 gaagtcatcg atgctcgtct gcaaggtccg gactgggcgg tgaccgacct cgatctcagc
    22561 ttgcgcaaca tgaccttcag taaagatgac tggcagacac aggaaggcaa actgtcgatg
    22621 aacgccagcg agttcattta tggttcgctg catttatttg acccgattat aaacgcggaa
    22681 ttttccccgc agggcgtagc gctgcgccag ttcaccagcc gctgggaagg gggtatggtc
    22741 agaacgtcag ggaactggct gcgtgacggt aaaacgttga tccttgatga tgcggcaatt
    22801 gccgggctgg aatatacctt gccgaaaaac tggcaacagt tgtggatgga aacgacaccc
    22861 ggttggttaa acagcctgca actgaagaga tttagcgcca gccgcaatct gatcattgat
    22921 atcgaccctg acttcccgtg gcagctcacc gcgctcgatg gttacggtgc caacctgacg
    22981 ctggttaccg atcataaatg gggcgtctgg agtggctcgg cgaatctgaa tgccgccgcc
    23041 gcgacattca atcgtgttga tgttcgtcgc ccgtcgctgg cgctgaccgc caacagcagc
    23101 acggtgaata tcagcgaact gagtgcattt actgaaaaag gcattctgga agccaccgca
    23161 agtgtttcac aaacgccaca acgtcagacc catatcagcc tgaatggacg cggtgtgccg
    23221 gtgaatattt tgcaacaatg gggatggcct gaattaccgt tgactggcga cggcaatatt
    23281 cagcttaccg ccagtggaga tattcaggcc aatgccccgc tgaaacctac ggttagcggg
    23341 caattgcatg ccgtgaacgc cgcaaagcag caagtgactc aaaccatgaa tgcgggcgtc
    23401 gtttccagcg gtgaagttac atcgacggag ccggtgcggt aaacatcaat ctcgccgggt
    23461 acgactcaga cgtacccggc attccgtcaa tagattacaa cgtaattgtc agcgcattcc
    23521 cttgaggcgt caccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    23581 ttactttcac aacattgcgc aagcacagcg tccagttctt cgcctcgccc gcaccagtca
    23641 cagtaatcgt gttgccagta cgtgctgctt ttaaagtaaa gatcaccgat ccgtcagcag
    23701 caggcacttc acagacggct tcatgcccgt cttgcagatt aaagaggtgg aatgccgtgc
    23761 cttcgtgcca cacgtaatcg ggacgttgat cgttgttgcc cagcgccagt agagtgttat
    23821 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    23881 cgagttcatc gttgtgccac aggtgtgtcc agcgaccttc cggcaggtag aactgcacat
    23941 cgcccgcttc agtgaacacc ggcgcaacca tcacgttgtc gcctaacatg tattgacggt
    24001 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    24061 taccccgcgc gttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    24121 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    24181 aagatttgct accgtgtaaa cggctatggc tggagagcaa accaaacgcg caccagcgtt
    24241 tgtaaacgtg cgccggagcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    24301 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    24361 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    24421 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    24481 tgtagatgta cgcataatgg ttatgcattt tctgcgaatc ggaaccgtca aaccactgaa
    24541 catcaattgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    24601 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    24661 ccagacctgg ctgccattta tcccactgcc atagcgaacc gtccgggcgt ttgagtaaat
    24721 agcctttctc ttgtaactct ttaaagacgg gggatttttg accgatatag gggttaatcc
    24781 agacgcagat tttcagccct ttcgctttca ggcggcggat catcccttcc gggtcaggga
    24841 aagtcagcgg gtcccactca aaatcgcacc actggaaggc tttcatccag aaacagtcaa
    24901 agtggaaaac atgcagcggc agattgcgtt ccgccatacc atcgataaag ctgtttaccg
    24961 tcgcttcgtc gtagttggtg gtaaatgaag tggttagcca caggccgaag gaccacgggg
    25021 gcggcagcgc cggacgacca gtaaagcggg tataacgatc aagtaccgct ttcggcgtcg
    25081 ggccgtcgat aacaaagtat tcgagatatt cactctcaac gctgaactgc actttggaga
    25141 ctttctccga tcccacttca aaagagacac actggggatg attgaccagt accccataac
    25201 cacggttagt catgtagaac gggatatttt tatacgcctg ttcagtactt gtgccgccgt
    25261 cccggttcca ggtctctacc gtctggccat tgcgcaccag ggcagtaaag cgctctccca
    25321 gaccgtaaac tgtttcgcca acgccaagat caagccgctc aaacatataa ttgcgttgat
    25381 tattcgtgtc ctgcacgtag ccattatttt tcacctgact accggtaata cgttcgccgt
    25441 tgcgcagaaa atccagtgac cagaactcac ctttgctgac acgcgcgctt aagttgccac
    25501 ttttaaactc agcataacgt tctgtgtttt cgattgtgac cttcacgtcc tgcaaaatat
    25561 tgagcggata atgaggaccg ttattcagcg ccccctgaaa atgctcaatc cgcacaccga
    25621 caataccttc ctgtggggag aaaaagcgca gcgtaaataa aggcgtatca agctgccagg
    25681 tacgttcacg cacatcacgg ggggcagcat agaccaccat ttcattatcc tgctgttcaa
    25741 cctcgaatac ctgaagcggg tgaatcaaat tgaggccagg ttgaatcaac cagtttccat
    25801 cgctaatttt cattgccgtt ccttagttct gcacttcttg agaggtgaat tgttgctggc
    25861 aacggcgttt accctgagcc agctgttcca taacggtttt cagattgtgc gtcgtgagtg
    25921 agtagtagcg tttagcgata atcgcgctca gcaaataaca gatcgccgga acaatcgtga
    25981 atagcgcaat aatgatgcta atcgtggcgc tgttctgcgc tttttctgcc gcatcatatc
    26041 cgccataagc cagcatccag ccgataagag cgccgccgaa ggccaacccc agtttgagca
    26101 caaacagcgt gccagcaaaa ctgatcccgg tcaggcgttt accattgcac cactcgccgt
    26161 agtcgacggt atcgaacatc attacccact ggataggtgt caccagttga tgcaacacac
    26221 caatcacgaa gatgaagaca aacatagtga tgctggcctg catgggaaca aagaacatcg
    26281 cgaggctaat cactgccagc agggcgttcg tccaccagaa gatagtgact ttacatttcc
    26341 agtcggtcag aggttttgcc agtgcggaac caatcaggtt acccacgcaa taagtggtga
    26401 gaaaagcgac aaacacttcc ggcgtgccca aaatccatgt gacgtaatac atcatcgccc
    26461 caccgcgtac gcacaccgcc aggatattga aaatggttag taaaccgaca atccgccact
    26521 ggtcgttttg ccagatatca cgtaaatctt cccgcataga cgttgttgta ggtggtgctt
    26581 caacgcgttc tttagtggtg aagaaacaaa atgccagcat catgaatgcc accacggaaa
    26641 ggaccacgat accgccctgg aaaccgagtg gtttattatc accgccaatt aaattaacca
    26701 gtggcatcat cagaacagta gaaagcatgc ctcccgcggt cgccagcaca aaacgccagg
    26761 attgcagcga gatacgctga gtcgggtcat tggtgattac accacccaat gcgcagtaag
    26821 ggatattgac gacggtataa agtaaggtaa gttagggtgt aagtaattgc tgcatagatc
    26881 attttgccgt tcatactgag atctggcgtg ctataggcca gtacacagac gatcccgaat
    26941 ggcagtgcgc caaacagtac ccacggacga aatttacccc agcgagagcg cgttcgatcg
    27001 gccaacaacc ccatgcaagg atcggaaatc gcatccagtg cacgagcgac caaaaacatg
    27061 gttccgacaa atccggcagg aatgccaaaa atatcggtat aaaagaacat catatataac
    27121 attacgttat cgaaaataat gtggctggcg gcgtctccca tgccataacc aattttctct
    27181 ttaacggaca acacttcact cctcatctgc ttttccttga tgctataggg gtgctgagac
    27241 cgataacgca ttttgtaaac catccctgtg agagcgaata ttccgatatc tggttattaa
    27301 tttatgattc ttgttttatg tgatcgtggt agcgttaatt ccgctcatat atcattgtaa
    27361 aatatgggtt ttatataaat tataatgctt tcgtgataat acgctgcgtt tattaggcgg
    27421 aaaaaactga tctgggggat gtagaaactc aaggaagtag ctataatgcg ccccgcctcc
    27481 atgtagcaat cgaggcgcgg aagatcgtcg tctccggtga ggcggctgga cttcaaatcc
    27541 agttggggcc gccagcggtc ccgggcaggt tcgactcctg tgatcttccg ccagtttacc
    27601 tcttctgaac tctcccaatg tcaataaatc tcttctaatc ccttgtatat cagtatttat
    27661 ttgtatcctg aggtcaacct acttcacccc gtatcaagtt tcttttgggg gtactttagg
    27721 gggtgttatt tcctattgaa atacaagcac ccccataata ggaaaaatgt gatacataac
    27781 aacggctttt aacaatatct ttgttataca aggggtgtat tgggggtatc aggttagtta
    27841 ctggcagaaa tccccctcag gaacatgaat tcatcaaaag ccggaggatg tcatggcatt
    27901 aacgatatca aagtgaaaac cgcaaaaccc aaagacaaac cctataagct tgctgatggc
    27961 ggcggtatgt atctactgat taacactaat ggttctaaat actggcgcat gaagtaccgc
    28021 tttgctggta aagaaaagat gctttctatt ggcgtatacc ccgatgtaac gctagccgat
    28081 gctcgcgaga aacgcagtga agccaggaaa ctcctcgctg ctggaggcga tccaggggag
    28141 gcaaaaaagg aagaaaaaat cgcgcagcaa atgagtctga aaaatacgtt cgaggctatt
    28201 gctcgcgaat ggcaccagtt aaaagctgat cggtggtcat tacgctatcg tgatgaaatc
    28261 attgatacct ttgaaaaaga catttttccc tacattggca agcgccctat tgccgaaatc
    28321 aaaccaatga agttactgga agcactgcgt aaaatggaaa aacgtggagc tctggagaaa
    28381 atgcgcaagg tccgccagcg ctgtggtgag gtgttccgct atgcaattgt tactggccgg
    28441 gcagactata acccagcccc cgatcttgcc agtgctttag ctacaccgaa aaaagtacat
    28501 tttccctttc ttactgctaa tgaacttccc cacttcctca acgatctggc gggttatacc
    28561 ggaagtataa tcactaagac agctactcag atcattatgt taaccggcgt acgaacacaa
    28621 gaactgcgtt ttgcacgctg ggaagatatc gattttgaga caaaattgtg ggagatcccg
    28681 gcagaagtaa tgaagatgaa gcgacctcat attgtgccgc tctctgagca ggtcattatg
    28741 ctatttaagc agcttgaacc aatctcaaaa catcatcccc tagtctttat cggtagaaat
    28801 gatccccgca aaccaattag taaggaaagc attaaccaag taattgaatt acttggatat
    28861 aagggaagac tcacagggca cggtttcaga cacaccatga gtacaattct gcatgaacaa
    28921 ggctttaatt ccgcctggat tgaaatgcag ttagctcatg tggataaaaa ttccatcagg
    28981 ggtacctata atcatgccct gtatctcgat ggccgccgtg aaatgatgca atggtacgct
    29041 gattacattg attcactctc cagccgggaa agttaagtca gaaatacttt tccctgatgt
    29101 tttcctcaat taagtatttt atcctctggc ctacaaggct ggaggatttt tgttattata
    29161 ttattttttt gcgtcttcaa acttattaca aaaacataat taaaaacaaa ttgacatttt
    29221 taaattggca attacaaaat acagccttgc agttatttcg atacctttag caaaggaata
    29281 gttgattcag atataattta gcatttcaat catctgacat cattataaaa aatgagattc
    29341 acataaagat aaaattattg ttctcaagga aagcatgaag gggcttcgta agcaatattc
    29401 attcggtgcc aagacaggac ttgatacaca gaatctcaac ttttatgctc tcagactata
    29461 tcaccacatg ttttacaggc tcagaaaatg aacgatagat tatgctttga agtgcacgat
    29521 aacaagggtt atttcgtatt tccggatacc tggtttggcc cattgctggg ggagtttgaa
    29581 gaagtgcttg atgcatacga cgctgatgaa atcagcgaga cgagttacat caacaaactc
    29641 cgacgccttg ctcaacggga gccggacttt attgatatac acgctcatct ggcatatgca
    29701 tttctggaac agaatgcccc gcgtaaggca ctgaacgccg ctcttaaagg actggcggca
    29761 ggtaatcgta tcatcccgga aagtttctgt ggtgaaataa tctggatgca tcccgaaaac
    29821 cggccttatc tgcgcgcgct ttatgcagca attctcgcaa atgtgcactt acagcgccat
    29881 caggatgcgg ttatgctcac tgataaaata ctggcataca atcctgaaga taatcagggg
    29941 gctcgctggt tgttgggttc agagctgtta cgcaccggcg atcatgagcg agccttcagt
    30001 gtactgaaag agcatgctga tgaattttca ccttactggt atgaactcgg tctcctgcat
    30061 tttctgaacg gggaacatgt taaggccgcc acagcttttc gccatggctt tgccacaaac
    30121 acttacatcg cagaaatgct gtgcggaaat ctgcatccat ttccacttgc tgtgtggcat
    30181 gacttctccg gctctctgga tacggcagaa gattactatg caacatactc accattatgg
    30241 ggacaatatc cggaagcatt gctgtttgta aactggctct ataaccattc ctccgtgctg
    30301 catgaacgat cagaaatcat taaatgcgcc gaaatgctga ttcaggaaga tgatttcgaa
    30361 atatgcgaaa gtattttaag acagcaggag cacctgtgga aaagaattga caagacactt
    30421 tctgaggaaa ttgtacagaa atgcagaaat atgaacggtg aatatatctg gccctggata
    30481 ttgccctttt ccgcagcagg gatgaaacat tcaagcatcc agtatcagta gatattgcat
    30541 tagtgtaact ttaaaacaag taataattta ttatccaata ataacactgt gttccttccc
    30601 caggtgatta ccctggggtt attttctgaa agtttccttt gcaaaaggat agcgttgtaa
    30661 attactttaa cgaattactc catgaactca ttaaagttct ttcgtaatac tttatcgtca
    30721 aacacgttat agctggcatc aaaaaccaca gatttaccct gccagcgggt aatcccctcc
    30781 tctggcgttt tcggttgaac tggtatcccc gtccaacaca ggcagccatt ataaacagta
    30841 aaaaattgta ttcgctgtta tgaaatcaat agtcctgacg tactgttgaa tgtgcttgtg
    30901 ttctgaacct cgtacaacca gtacatttga tatcgaatta taaacaaaac attgcatcca
    30961 acttttttaa tatggacaat agttgtccat acggtggttt tatattcgat aaatcaatgg
    31021 atttcattga gtcacaggac tggtaatgga tgaaaatgct ttaaggtttg cctcatactg
    31081 gcgcaactcg cttgcagatg ctgagtcagg aaagggctgt ttt
//
LOCUS       DAJTYH010000041        30193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTYH010000041 DAJTYH010000000
VERSION     DAJTYH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1478
                     /locus_tag="OO609_001857"
     CDS             <1..1478
                     /locus_tag="OO609_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6633294.1"
                     /translation="FPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTPCTD
                     GYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLRDAR
                     FMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEHYFS
                     EQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQAKGT
                     LRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVEHVC
                     RLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWVINE
                     LARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATVRSL
                     LNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAIHRS
                     SKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1475..2431)
                     /locus_tag="OO609_001858"
     CDS             complement(1475..2431)
                     /locus_tag="OO609_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6633295.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2582..3697
                     /gene="macA"
                     /locus_tag="OO609_001859"
     CDS             2582..3697
                     /gene="macA"
                     /locus_tag="OO609_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6633296.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3694..5640
                     /gene="macB"
                     /locus_tag="OO609_001860"
     CDS             3694..5640
                     /gene="macB"
                     /locus_tag="OO609_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6633297.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5713..5937)
                     /gene="cspD"
                     /locus_tag="OO609_001861"
     CDS             complement(5713..5937)
                     /gene="cspD"
                     /locus_tag="OO609_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6633298.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6260..6580
                     /gene="clpS"
                     /locus_tag="OO609_001862"
     CDS             6260..6580
                     /gene="clpS"
                     /locus_tag="OO609_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6633299.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6611..8887
                     /gene="clpA"
                     /locus_tag="OO609_001863"
     CDS             6611..8887
                     /gene="clpA"
                     /locus_tag="OO609_001863"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6633300.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9290..9377)
                     /locus_tag="OO609_001864"
     tRNA            complement(9290..9377)
                     /locus_tag="OO609_001864"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9341..9343),aa:Ser,seq:gga)
     gene            complement(9631..9849)
                     /gene="infA"
                     /locus_tag="OO609_001865"
     CDS             complement(9631..9849)
                     /gene="infA"
                     /locus_tag="OO609_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6633301.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10134..10838)
                     /gene="aat"
                     /locus_tag="OO609_001866"
     CDS             complement(10134..10838)
                     /gene="aat"
                     /locus_tag="OO609_001866"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6633302.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(10880..12601)
                     /gene="cydC"
                     /locus_tag="OO609_001867"
     CDS             complement(10880..12601)
                     /gene="cydC"
                     /locus_tag="OO609_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6633303.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12602..14368)
                     /gene="cydD"
                     /locus_tag="OO609_001868"
     CDS             complement(12602..14368)
                     /gene="cydD"
                     /locus_tag="OO609_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6633304.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFTTLLAHRQEEI"
     gene            complement(14491..15456)
                     /gene="trxB"
                     /locus_tag="OO609_001869"
     CDS             complement(14491..15456)
                     /gene="trxB"
                     /locus_tag="OO609_001869"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6633305.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16000..16494
                     /gene="lrp"
                     /locus_tag="OO609_001870"
     CDS             16000..16494
                     /gene="lrp"
                     /locus_tag="OO609_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6633306.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16629..20657
                     /gene="ftsK"
                     /locus_tag="OO609_001871"
     CDS             16629..20657
                     /gene="ftsK"
                     /locus_tag="OO609_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6633307.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            20812..21423
                     /gene="lolA"
                     /locus_tag="OO609_001872"
     CDS             20812..21423
                     /gene="lolA"
                     /locus_tag="OO609_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6633308.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21434..22777
                     /gene="rarA"
                     /locus_tag="OO609_001873"
                     /gene_synonym="mgsA"
     CDS             21434..22777
                     /gene="rarA"
                     /locus_tag="OO609_001873"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6633309.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            22868..24160
                     /gene="serS"
                     /locus_tag="OO609_001874"
     CDS             22868..24160
                     /gene="serS"
                     /locus_tag="OO609_001874"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6633310.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24400..26843
                     /gene="dmsA"
                     /locus_tag="OO609_001875"
                     /pseudo
     CDS             24400..26843
                     /gene="dmsA"
                     /locus_tag="OO609_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            26854..27471
                     /gene="dmsB"
                     /locus_tag="OO609_001876"
     CDS             26854..27471
                     /gene="dmsB"
                     /locus_tag="OO609_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6633311.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27473..28264
                     /locus_tag="OO609_001877"
     CDS             27473..28264
                     /locus_tag="OO609_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6633312.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28300..28926)
                     /locus_tag="OO609_001878"
     CDS             complement(28300..28926)
                     /locus_tag="OO609_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6633313.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29241..>30193
                     /locus_tag="OO609_001879"
     CDS             29241..>30193
                     /locus_tag="OO609_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633314.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHH"
BASE COUNT         7437 a         7448 c         8157 g         7151 t
ORIGIN      
        1 ggttcccgcc gggagatatc aacgggcgag aacatcatct gcatattatt ttgaccttcc
       61 gcgaatcgct gccaggccga catcgggttc gccgttatcg gccgctggaa gcgtgctgga
      121 cgccatgcac cgatggctat caccgtattt tttatcgtct ggaaggggag agtgcggaag
      181 acggcagcgt gatgacactg cgcagttttc tcgataaaga cggacatccg attgatgttg
      241 aggatattaa cgatcaggca cgccatctgg tgcgtttaat gccggtgctg cgcttgcgtg
      301 atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc aaatgtcccc aatgtggaag
      361 tcaccgcgcg ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg caaaatctct
      421 ctgatggaca gattcgtcag ggactttccg caatggtgca gttgcttgag cattatttct
      481 ctgagcaggg ggctggacag gcgcgatatc gtttaatgcg gcggcgggcc agcaatgagc
      541 aacgaagctg gcgctatctg gacatcatca accggatgat tgaccgacct ggagggcgct
      601 cgtatcgggt tattttgctc ggcctgtttg ctactttatt gcaggcaaaa ggcacattgc
      661 gactggataa agacgcgcgt ccgttattgc taattgaaga tccagaaacc cgcctgcatc
      721 cgattatgct ttccgttgcc tggcatctgt tgaatctttt gcctttacag cgtattgcta
      781 ccaccaactc tggcgagctg ctttcgttaa ctccagtgga acatgtttgc cgcctggtgc
      841 gtgaatcttc tcgtgttgcc gcctggcgtc tggggccaag tggcttgagc accgaagaca
      901 gccgccgcat ctcttttcac attcgtttta atcgtccgtc atcgctgttt gctcgctgct
      961 ggttgctggt ggaaggggaa acggaaacct gggttatcaa tgaactggcg cgtcagtgcg
     1021 gacatcattt tgatgccgaa gggatcaagg tcattgagtt tgcccagtcc gggctaaagc
     1081 cactggttaa atttgcccgc cgaatgggga ttgaatggca tgtactggtc gatggcgatg
     1141 aagcagggaa gaaatatgcc gctacggtac gcagcctgtt gaataacgat cgggaagccg
     1201 aacgagaaca tttaacggcg ttaccggcgc tggatatgga acattttatg tatcgccagg
     1261 gattttccga tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg atgaatctac
     1321 gcaaaattat ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc attgaagtgg
     1381 caatggaggc aggacgtcgt ggtgtggact ccgtaccaac gctgctgaaa aaaatgttct
     1441 cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc gtggccattt
     1501 gtggctgaat agcgtcgagc atctcatagc gccgacggta ttcagcccgt tttttactgg
     1561 cgatttcggc aatctctttt cgtgctatct gtgctggaag gcggtaatgg cgttcagcat
     1621 cacatacgcc gccaaccgac tcccagaacg cgttgtaatc agcatggatc ttgccttctt
     1681 tatcgcgata acgcaggctg cggtaaatat gcgtttcatt gctgacggca ataatctgct
     1741 ctacctgcaa acgttgggca aacagacagg ccgcttccat cacgaggcgt ttgggaaata
     1801 gcccgtggca ggctttcgtc gcattctgga tttcctgatg tggaatttcc cattttgcgc
     1861 cttgcagtcc gccaataaac atcgttcttt tcccctgata ttcacacagg gtaaacgtga
     1921 tctctgccag aggaatacct tcgctgttgc ggaacaggat tgtgctgtca ccttctttat
     1981 ccattgagat catcatggtc agctcaagcg tgaactgctc accgtttttg ccttccagtt
     2041 tagccagttg cagcccgggg gtattcaaat ataagctgaa ttcttccgcc gacatacacc
     2101 cgcggagtaa cgcataatgg taacgtaacg cctccagcaa ttgcttacgg ctaagattcg
     2161 cggcaaggta agggcgatgc agacgcacag gcagtcgcgg ctggcgcgtt aacaacacat
     2221 tgagattagg ccagtgggaa agttcgttca tccactcaac gcttaaacgc ggcataatca
     2281 acgagcgcag caaaaatttc tggcgaaaac tacagcgatg ccagaattta cccggccgac
     2341 actgtccacg tgccagacta agaaaaagtg acaggctgct gagagattca gatggcgtaa
     2401 aggtccgttc agttagctgc gacatattca tgaaatcaat ggttatacat gacgtcgatt
     2461 tcaccattgc gtatcttaac caaacatcaa tagtgtgatt actaacgtaa attttagggt
     2521 tttgttgata tttcgttgaa gttaatgacc cggattggca tatggagtat tcagaatatt
     2581 tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga tagtcatcgc
     2641 cggactgatt acgttatgga gaattcttaa cgcacccgtg ccgacttatc agacactgat
     2701 tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg acgcgctgcg
     2761 taaggttgac gtgggcgcgc aggtcagcgg tcagttgaaa acgctgtcgg tggcgattgg
     2821 cgataaagta aaaaaagacc agcttttagg ggtcattgat cctgaacagg ctgaaaacca
     2881 gatcaaggag gtcgaagcaa cgctgatgga gctacgcgcg cagcggcagc aggcggaagc
     2941 ggagctgaaa ctggcgcggg tgacgtattc ccgtcagcaa cgtctggcac aaacgcaagc
     3001 ggtttcactg caggatctcg ataccgccgc gacggagatg gctgtgaaac aggcgcaaat
     3061 tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcctct ctcgatacgg ctaaaaccaa
     3121 tctcgattac acccgtatcg ttgccccgat ggctggggaa gtgacgcaaa tcaccactct
     3181 gcaaggccag acggtgattg ccgcacagca agcaccgaac attctgacgc tggcagatat
     3241 gagcactatg ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc tgaagccggg
     3301 gcaaaaagcc tggtttacgg tgcttggcga tccactgacg cgctacgagg ggcaaatcaa
     3361 ggatgtgcta ccgacgccgg aaaaggtaaa cgacgctatt ttctattacg cccgttttga
     3421 agtacccaac cccaatggtt tgctgcggct ggatatgacc gcgcaagtgc atattcagct
     3481 caccgatgtg aaaaatgtgt tgacgatccc tctgtcggcg ttaggcgatc cggttggcga
     3541 taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg aagtgacgat
     3601 tggcgcacgt aacgataccg atgttgagat tgtcaaaggg ctggaagcgg gcgatgaagt
     3661 ggtgattggt gaggccaaac caggagctgc acaatgacgc ctttgctcga attaaaggat
     3721 attcgtcgca gctatcctgc cggtgatgag caggttgagg tgctgaaggg catcagcctc
     3781 aatatttatg cgggtgagat ggtcgcgatt gttggcgctt cgggttccgg taaatcgacc
     3841 ctgatgaata ttctcggctg cctggataag gccaccagcg gcacctatcg cgtagccggt
     3901 caggatattg ccacgctgga cgccgatgcg ctggcgcaac tgcgccgcga gcatttcggc
     3961 tttattttcc agcgttacca tttgcttccg catttaaccg tcgagcagaa cgttgaagta
     4021 cccgccgtct atgccggtct tgagcggaaa cagcgactgc ttcgtgccca ggagttgctg
     4081 caacggctgg ggctggaaga ccgtacagag tattatccgg cacagctttc gggtggtcag
     4141 caacagcgcg tcagcatcgc gcgggcattg atgaacggcg gtcaggtaat tcttgccgat
     4201 gaaccgaccg gcgcactgga cagccattct ggcgaagagg tgatggcgat cctgcatcag
     4261 ctgcgcgatc gcgggcatac ggtgattatc gtcacccacg atccgcaggt cgctgctcag
     4321 gccgagcggg tgatcgaaat tcgcgacggc gaaattgtgc gcaatcctcc cgccattgaa
     4381 aaagtgaatg ttgctggcgg gacggagcct gttgtcaata cagtgtctgg ctggcggcag
     4441 tttgtcagcg gttttaacga ggcgctgacg atggcatggc gggcgctggc agcgaataaa
     4501 atgcgtactt tactgaccat gctggggatt attatcggta ttgcgtcggt ggtttccatt
     4561 gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg atattcgttc tattggtacg
     4621 aatactattg atgtctatcc cgggaaagat tttggcgatg acgatccgca atatcagcag
     4681 gcgctgaagt acgacgactt aatcgccatc caaaaacaac cgtgggtcgc ctcagccaca
     4741 cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc tgccagtgcc
     4801 aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga aggaaacacc
     4861 tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg ttctcgacag taatactcgc
     4921 cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt cggcaatatg
     4981 cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag cagtaaagtg
     5041 ctgcgtgtct ggctacctta cagcacgatg tccgggcgag ttatgggcca gtcgtggctt
     5101 aactccatta ctgtcagggt gaaagaagga tttgacagcg ccgaggcgga acagcaactc
     5161 acgcgtttac tttcactgcg ccacggtaag aaggatttct ttacctggaa catggacggc
     5221 gtcttgaaaa ctgttgaaaa gaccacacgt actttacaac tgtttctgac gctggtggcg
     5281 gtgatttcgc tggtggtggg cggtattggt gtaatgaata ttatgctggt gtcagtgacc
     5341 gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag cgatgttttg
     5401 caacagttcc tgatcgaagc cgtactggtt tgcctggtcg gtggcgcgtt gggaataaca
     5461 ctgtcactgt taattgcttt caccttgcag cttttcttac ccggctggga gattggtttt
     5521 tcaccgttgg cgctgctgct ggcgtttctc tgctcgacgg ccaccgggat tttatttggc
     5581 tggttacccg cacgaaatgc ggcacgactg gatccagtag atgctctggc acgggagtaa
     5641 tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga tgtacacaat
     5701 gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa cactggcgtg
     5761 attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt ttagcgttct
     5821 gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc cgccttcagg
     5881 gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct tttccatgct
     5941 tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc agagaggggg
     6001 ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg agccgtcaag
     6061 catttgacag gggccagggg gcaggtatga atcaaaaatt tgaagcagtt aacgctattg
     6121 acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttatct atcatgtgga
     6181 gtagattggt caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa
     6241 ccgaagatga taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc
     6301 ggaagaaaaa gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa
     6361 tgatgattac actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga
     6421 tgtagaacgt gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg
     6481 agtctttacc gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga
     6541 gaatgagcat ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg
     6601 ggaggtgcct atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc
     6661 gcgcgagcac cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa
     6721 cccatctgcc cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga
     6781 actggaagcc tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga
     6841 cacacagccg acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccagtc
     6901 ctccggtcgc aatgaggtta ccggtgcaaa cgttctggtc gctatcttca gcgaacagga
     6961 gtcgcaggcg gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg tggtgaactt
     7021 tatctctcat ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc
     7081 aaacagcgaa gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa
     7141 ccagcttgcg cgtgtgggcg gaatcgaccc actaattggt cgtgagaaag agctggagcg
     7201 tgctattcag gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg gggaatctgg
     7261 tgtcggtaaa accgcgattg cagaaggtct tgcctggcga attgttcagg gtgatgtgcc
     7321 ggaagtgatg gctgactgta cgatttactc tctcgatatc ggttctctgt tagcgggcac
     7381 aaaatatcgc ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcagga
     7441 cactaacagc atcctgttta ttgatgagat ccacaccatt atcggtgcgg gtgcagcgtc
     7501 tggtggtcag gtcgatgcgg ctaacctaat caaaccgttg ctctccagcg gtaaaattcg
     7561 cgtaattggt tcgacaacct atcaggagtt cagcaacatt ttcgagaaag accgtgctct
     7621 ggcgcgtcgc ttccagaaaa ttgatattac tgaaccgtcg atcgaagaaa ctgttcaaat
     7681 catcaatggc ctgaaaccga agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc
     7741 ggtgcgtgcg gcggtagagc tggcggtgaa atacattaac gatcgtcatc tgccggataa
     7801 agccattgat gttatcgacg aagcaggcgc tcgcgcacgt ctgatgccgg taagcaaacg
     7861 caagaaaacc gttaatgtgg cggatattga gtccgtggtg gcccgtattg cacgcattcc
     7921 agagaagagt gtttctcaga gtgaccgcga taccctgaaa aaactcagcg atcgcctgaa
     7981 aatgctggtc ttcggccagg ataaagccat tgaggcgctg actgaagcca ttaagatggc
     8041 gcgtgcaggt ttaggtcacg aacataaacc ggtcggttcg ttcctgtttg ccggtcctac
     8101 cggggtcggg aaaacagagg tgacggtaca gctttcgaaa gcgttgggca ttgagcttct
     8161 gcgctttgat atgtccgagt atatggaacg ccataccgtc agccgtctga ttggtgcgcc
     8221 tccgggatac gttggttttg atcagggcgg tttgctgacc gatgcggtaa tcaagcatcc
     8281 acatgcggtg ctgctgctgg acgaaatcga gaaagcgcat ccggacgtgt tcaatattct
     8341 gttgcaggtg atggacaacg gtacgctgac cgataacaac ggacgcaaag cggacttccg
     8401 taacgtggtg ctggtgatga ccactaacgc cggggtacgg gaaactgagc gcaaatccat
     8461 tggtcttatc caccaggata acagcaccga tgcgatggaa gagatcaaga agatctttac
     8521 accggaattc cgtaaccgtc tcgacaacat tatctggttc gatcatctgt caactgacgt
     8581 gatccatcag gtggtggata aattcatcgt cgagttgcag gttcagctgg atcagaaagg
     8641 tgtttctctg gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg
     8701 ggcaatgggc gcacgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc
     8761 caacgaactg ctgtttggtt cgctggtgga cggcggtcag gtgacggttg cgctggataa
     8821 agagaaaaat gagctgactt acggattcca gagtgcacaa aagcacaagg cggaagcagc
     8881 gcattaatct gattgtcagg taggttggtg aagtccgtaa tctcgtcagg ggttacggac
     8941 tttttattta tggggtggaa cggtgaaaac cctatttttg gaggtgaagg taagttgttg
     9001 ataattagtg ctgctggaag gtaaggataa aaaagggtgc tgcaggagaa tgggatggtt
     9061 ttgctttatt aacaacgggc taaacgtgta gtatttgagt tcactgccgt acaggcagct
     9121 tagaaatcga cggggtgcgg taaaaccttt gcgaacgcgt tcactgccgt acaggcagct
     9181 tagaaattca caggtaacat actccacccg cccaccatgt tcactgccgt acagacagat
     9241 aaaatgcgaa aaaaaagctc gcactttcgt acgagctctt ctttaaatat ggcggtgagg
     9301 gggggattcg aacccccgat acgttgccgt atacacactt tccaggcgtg ctccttcagc
     9361 cactcggaca cctcaccaaa ttgttttgct accaaacctc atgggtggca acggggcgct
     9421 actataggga gttggagtaa aacggtcaag aagaatttta atgataatta ttgtttgctc
     9481 atactgtaaa caacttgtgc agtatatcta catcgagaca agttatggac ttatacttcc
     9541 aaagtacttc atacatatca caaaataaaa aggccggtta aaccgacctt ttactcgttc
     9601 tttctcttcg cccatcaggc ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt
     9661 gctcaggtcg tacggggtca gttcaacagt cactttgtcg cccgtcagga tgcggatgta
     9721 gtttttgcgc attttaccgg agatgtgtgc agtaaccacg tgaccgtttt ctaactctac
     9781 gcggaacatg gtattaggca acgtttcaag aacggtacct tgcatttcaa tattgtcttc
     9841 tttggccatc taatcctctg gggtatcact accgtaattt gaaccggcaa gataatgccg
     9901 aagttctgta aataagtaaa gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt
     9961 actccgaaaa cacacggcta agccgcacca aaagcgcaac gtataaggga gcggtgagat
    10021 aaacgatggg cgttacctga cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa
    10081 ccaactctgc gaccgcaatt ataacactct ggggagaaat gtgccgaaaa cattcattct
    10141 tgtggtgaaa acaagcaccg tggtacccag aaattattcg gcaatcgtcc gaggcgcatt
    10201 tgattgagat aattaaggta atcccggcgg ggaatttcgc aggcaccaag cgatgctgtg
    10261 tgatcgttaa ggacctggca gtcgataagc ttaccgccat gaccgataaa ttcctcacag
    10321 aataccagaa gcgccgtttt agacgcattt tccatccggc tgaacatgga ctcgccacaa
    10381 aatagcgttc cctgggccac gccgtacata ccgccgacaa gctcatcttc acgccagact
    10441 tcaatggagt gggcatgacc gagttcgtga aggcgatggt aggcttcgac cacgccacgc
    10501 gtgatccagg ttccttcttc gcgatcgctg gcacagcctt caatgacctg accaaaagcg
    10561 taattcatcg tgacacgata gggcgagcgt ttatgaaatc gcttcatact acggctgata
    10621 tgcagtgatt ctggccatag caccgcgcgg ggatcgggcg accaccagag gatggggtcg
    10681 cctggagaaa accacggaaa aataccacgc tggtaagcca ttaacaggcg cgcagggcta
    10741 agatcgcccc caagtgccag caggccgtta ggctcacgta atgcgccttc cggggaaggg
    10801 aaggctattg aatggcgaga aagctgaacc aagcgcatga ccgcaaaact ccacgcaagt
    10861 cggatcgttc aataatagct tacaaaccct gcttgaactg gtaataacgc ccctgtctgg
    10921 caagcagttc tgcgtgagta ccttgctcaa taatttgccc gttgtccatc actattattt
    10981 gttggaaacg agagagtccg cgaagtcgat gggtgaccat taacaccgtt ttctcacgca
    11041 tcatttttgc aagcaattca aggatctggc tttcggttgt ggcatctaag ccttcggtag
    11101 gttcatccag caacaccagt ggcgcatcat gtaacagcgt acgggcgata gccagacggc
    11161 gcagttcacc accggagagc tggcgtccgc cttcacctaa ccaactgttg agacctgcat
    11221 cctcgagcag cttctccaga ccaacgcgac gcaagatctc cgacagagcc tcatcactac
    11281 tgccaggcga ggcgagtaac agattatcac gcagtgtggc gctaaacaga tgcactcgct
    11341 gaggaacaac gctgatggtc tgtcgtagag ccgcttcatt caggctggct atggggctat
    11401 cgttaagcaa aatctcgccc tgttgcgggt cccatgcgcg ggtcagcagt tgtaacagtg
    11461 ttgatttgcc gcatccggtt cgcccgagaa tcgctatatg ttccccggcg tttacctgaa
    11521 gagaaatccc tttaagtgcc tgttgggatt gctccggata agtgaactga acatcccgta
    11581 atgtcagcga aacgcgatcg gcaacacgag tttgggtatc agggaaggtg acctccggtt
    11641 tttgatccgt taagtcagag atacgtacgg cagaggcaat gacttgcccc agatgctgaa
    11701 atgcacccgt tactggtgcc agtgcttcaa acgcggctaa cgcacaaaag acaaacaggg
    11761 caattaatgc gccgggttga gcattgccgc caacgccgcc agacgccatc cacagcatca
    11821 ggatcaccgc taacgcgcca atgagcagca ttatcgcttg cgacaatgcg gtcagttcag
    11881 attgacggcg ttgcgcttcc agccattgaa tttctgtatt ctctagttgc gtgcgataac
    11941 gatcgctggc accaaaaatg gtcagctcag cttgcccttg cagccaggcc gtcagttgtt
    12001 ggcgatactg tccgcgaaga tgagtcagat tttgcccggt gctttttccc gcacgataaa
    12061 acagcggtgg catcaggaaa agcgtcagta acataacgct gcccagcgta aaggcgaggg
    12121 tgaaatcaag gaaacttaac ccgattgtca ccaccataat caccacaaaa gcgcccacca
    12181 gcggcgagat aacgcgcagg taaagatgat cgagcgtatc aacatccgcc accacgcggt
    12241 tgagcaattc gccctgacga tagcgcgcca gtccggcagg ggagaggggc aacaatttgc
    12301 tgaaggtgta aatgcgcaga tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac
    12361 gttcaaaata acgcccggca gtacgggtga ttgcggcacc acgcacgccc gcagcgggta
    12421 gcatatagtt gaagctgtac agtccggcaa cccccgcaac cgctgaggcc gagaggaacc
    12481 agccggaaag tgtcaacaga ccgatactgg cgagcagcgt cacaattgcc agcacaatac
    12541 caagacttaa catccattta tgacgtttat acagtgccag atagggtagc aaagcgcgca
    12601 tttaaatctc ctcctgacga tgggccagta atgtggtgaa tgggccacca gccacactta
    12661 attccgcgta acgtccttgc tcaataatcc ggccatcctg cataacccaa atgacatccc
    12721 agtcagcaag atcttctaac tggtgggtga ccattaacgt tgtctggcgc agagaggcgg
    12781 cattcagcgc ctccattacg cgctgttcac tgtgagcatc aaggctggca gcgggttcat
    12841 ccaacagtaa tagcgaacag ggatttagta acgcacgggc caccgccacg cgctgcgcct
    12901 gccccacgga aaggcgggca gcctggtcgc caacaggtgt atcaacgcct tgtggcagga
    12961 gcggtagaaa ctcgctgacc caggcgttat ccagcgctgc ttgtaattct tgttcgctgg
    13021 catcaggtcg cgccagtagt acgttatccc gcaattttgc tgccggtaat tgtgggtttt
    13081 gcccaaccca ggagagatgt ttacgccatg attctggtga taaatcacgt aattctatcc
    13141 cgttgattcg taatgatccc tgatatgaga gaaaaccaga aagcgcgttc agcagtgagc
    13201 ttttacctga accgctgcgg ccaaccaaca ctgcacgttg gcctgctggc aaagtaaagt
    13261 tcagcggtcc ggccagcgtt ttaccttccg gcgacgtgat aaacagatcc tcggcctcaa
    13321 tggtcagcgg atcggtcaat gctaattccg cctcaccgcg ctgcggatgg gcgagcgggg
    13381 tttccataaa cgttttcaga ctgtcagctg caccaacagc ctgggcttta gcatgataaa
    13441 acgtaccgag atcgcgtaat ggctggaaaa actctggcgc aaggatcagg gccagaaaac
    13501 ctgcagccag cgtcacccca gtatcgtagt gaccaaaatc cagctcgccg agataggaaa
    13561 aaccaaagta gaccgccacc agagcaattg acagcgaggt aaaaaattcg agaatgccgg
    13621 aggataaaaa cgccagtcgt agcacttcca ttgtccgttg gcggaaatct tccgaagcag
    13681 aacgaatact ttcaatttca gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc
    13741 gcaggcgatc gaggaaatgc ccacttaagc gagcaagagc gagaaagtta cgccggttag
    13801 catcggcagc ccccattcca accagcgcca taaacaacgg aattagcggt gctgtgccca
    13861 gcagaatgag cgccgcagcc cagttagagg ggaagatcgc caccacaatc agcaacggca
    13921 ccgacactgc cagcgccatt tgcggcagat agcgtgcata gtaatcatgc atatcgtcaa
    13981 tttgctcgag taccagcgtc gcccagctcc ccgcaggttt accctgaatc cacgctggcc
    14041 ctgcttgttg cagacggtcg agaacctgac ggcggatggc aaagcggata tgctgcccgg
    14101 cgtgataacc cacccgttcg cgtaaccaga ccacccatgc gcgcaataca aaggtcagaa
    14161 ccagtaacgt aaagggaagc agcagggctt cacggggaat attctccata atcatatgtt
    14221 gcagaattcg cgccatgaac caggcctggg caatgatcaa tatgccgctt acaaagccca
    14281 gcagacgaga aatattcagc caacgttggg agatgacgct ttgctgtttt aaccagcggg
    14341 ttaactcttt ttgacgagat ttattcattg cacgcttagc aggtgagtta tcagaattat
    14401 ttgcagagca atgttacaac ggggaaaaaa taaaggcgac ccatagtcgc aaggtgtcgc
    14461 cttctttact tttgttactg atttgtaaaa ttattttgcg tcagctaaac catcgaggta
    14521 gcgttccgca tcaagtgctg ccatgcagcc agtaccggcc gaagtaatgg cctggcgata
    14581 aatgtgatcc atcacgtcgc ctgcggcaaa gacgccagga atgctggtct gggtggcatt
    14641 accatgaata cccgactgta ctttgatgta gccgttttcc agttccagct gcccttcgaa
    14701 aatcgcagta ttcgggctgt gaccgatagc aacaaacaga ccggcaacgt cgagtgactc
    14761 gatgttatcg ctgttttgcg tatcgcgcag acgaacgcca gtgacaccca tttgatcgcc
    14821 ggtcacttct tccagcgtac ggttggtgtg cagaatgatg ttgccgttct ccactttatc
    14881 catcagacgc ttaataagga ttttttccgc gcggaaaccg tcacggcggt gaatcagatg
    14941 cacttccgaa gcgatgttag acagatacag cgcctcttca accgcggtat tgccgccgcc
    15001 gatgaccgca actttctggt tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac
    15061 cccacggcct ttaaaggctt cttcagaggg caggccgaga tagcgtgcag aagctccggt
    15121 ggcaataatc agcgcgtcgc aagtgtattc gccgttatcg ccattcagac ggaacggacg
    15181 gttttgcaga tccaccttgt tgatatgatc aaaaatgatc tcagtttcaa acttggtggc
    15241 atgttcgtgc atgcgcgcca ttaataacgg accggtcaga tcgtttggat caccaggcca
    15301 gttttccact tccgtggtgg tggtcagttg gccgcctttt tccatgccgg taatcagcac
    15361 aggttgcagg ttggcgcgcg ccgcgtagac agcagcggtg tatcccgccg ggcctgaacc
    15421 caggataagc agtttactgt gtttggtcgt gcccatgaga tccccatagt tgttggcaga
    15481 caatgggcag gattgtaggg aatttacaga cgtaaaaaaa gagtatgacg attttgttaa
    15541 caatttgtgc aatcggcagc atcgataagc aggtcaaatt ctcccgtcat tatcacctct
    15601 gctacttaaa tttcccgctt tataagccga ttaaatgatg aataaacgcc cctgttaatg
    15661 aatatctggc atgttgtact aaaaatcgat gttttgcttt gacaatcccc tggtgttttg
    15721 cgaaaacatt cgaggaagaa aaaaacagta ttcttatatg cgcataacca tgtatgtaaa
    15781 taccatgttt accgtgctag tgaaatctac gtatggcgtg gacagacgcc attcgtgatg
    15841 tcgatagctg ccacaaggca acggtcttct caccgtagac ccaggcattg cgcgccgtga
    15901 atcttcatga tttcggtcta tcgtgacggg tagcgactct gaacagtgat gtttcagggt
    15961 cagacaggag tagggaagga atacagagag acaataataa tggtagatag caagaagcgc
    16021 cctggcaaag atctcgaccg tatcgatcgt aacattctta atgagttgca aaaggatggg
    16081 cgtatttcta acgtcgagct ttctaaacgt gtgggacttt ccccaacgcc gtgccttgag
    16141 cgtgtgcgtc ggctggaaag acaagggttt attcagggct atacggcgct gcttaacccc
    16201 cattatctgg atgcatcact tctggtattc gttgagatta ctctgaatcg tggcgcaccg
    16261 gatgtgtttg aacaattcaa taccgctgta caaaaacttg aagaaattca ggagtgtcat
    16321 ttagtatctg gtgatttcga ctacctgttg aaaacacgcg tgccggatat gtcagcttac
    16381 cgtaagttgc tgggggaaac cctgctgcgt ctgcctggcg tcaatgacac acggacatac
    16441 gttgttatgg aagaagtcaa gcagagtaat cgtctggtta ttaagacgcg ctaacacgga
    16501 acaggtgcaa aatcggcgta ttttgattac actcctgtta atccatacag caacagtact
    16561 ggggtaacct ggtactgttg tccgttttag catcgggcag gaaaagcctg taacctggag
    16621 agcctttctt gagccaggaa tacactgaag acaaagaagt cacattgaca aagttaagta
    16681 gcggccgccg ccttctggag gcattgctga tccttattgt cctgtttgcc gtctggttga
    16741 tggctgcctt actaagcttt aacccttcgg accccagctg gtcgcaaacg gcctggcatg
    16801 aacctatcca taatttaggt gggatgcccg gtgcgtggtt ggcagatacg ctgttcttta
    16861 tttttggcgt gatggcttac accattcccg tcattattgt cggcggttgt tggtttgcct
    16921 ggcgtcatca gtccagcgac gaatacattg attattttgc cgtttcgcta cgcatcattg
    16981 gcgttttggc gctcatcctt acctcctgtg gtctggcggc aatcaacgct gacgatatct
    17041 ggtattttgc ctccggtggc gtcattggca gcttactaag cactacgcta caaccactgc
    17101 tacacagtag cgggggaact attgcgctgc tctgcgtttg ggcagcgggc ctgacgttgt
    17161 tcaccggttg gtcatgggtg accattgctg aaaaactcgg cggctggatt ttaaacattc
    17221 tcaccttcgc cagtaatcgt acccgtcgcg atgatacctg ggtcgatgaa gatgagtatg
    17281 aagacgacga agagtatgaa gatgaaaatc acggcaaaca gcatgaatca cgccgtgccc
    17341 gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc ggaaaaattc attaatccga
    17401 tggggcggca aacagacgct gcgttgttct ccggtaagcg gatggatgat gacgaagaga
    17461 ttacctacac tgcacgcggt gtggctgccg acccggacga cgtcctattt tcgggcaatc
    17521 gtgcaacgca gccagaatat gacgaatacg atccattatt aaacagtgcg ccaattaccg
    17581 aacctgtcgc tgtagcagct gctgctacca cggcgacaca aagctgggct gcgccggttg
    17641 aacctgtgac tcagacgccg cctgttgcct ctgttgatgt tccaccttcg caacctacag
    17701 tagcctggca gcctgtaccg ggtccacaaa cgggagagcc ggttattgct cctgcaccgg
    17761 aaggttaccc acagcagtca caatatgcgc agcctgcagt gcaatataat gagccgctgc
    17821 aacaaccagt acagccgcag cagccgtatt atgcacctgc agctgaacaa cctgcgcaac
    17881 agccgtatta tgcccctgcg ccagaacaac cggtggcagg taacgcctgg caagccgaag
    17941 agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact tatcagcagc
    18001 cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag cctgttgtgg
    18061 agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccgctttac tattttgaag
    18121 aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat caaccgattc
    18181 cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct tctgttgcag
    18241 cagtacctcc agtagaagcc gctgccgctg tttctccgct ggcatctggc gtgaaaaaag
    18301 cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt ctggcaaata
    18361 gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca cgaccgaaac
    18421 gtatccgcgt gccaactcgt cgtgaattgg cgtcttacgg tattaagctg ccctcacagc
    18481 gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtatgattct ggcgatcagt
    18541 ataacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag ttcgcccaga
    18601 cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac gcagaagatg
    18661 cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa caacaacgtt
    18721 attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt gaattttcgc
    18781 caatgaaagc gttgctggat gatggtccac acgaaccgct gtttacgcca attgttgaac
    18841 ctgtacagca gccgcaacaa ccggttgcac cacagcagca gtatcagcag ccacaacagc
    18901 cggttgcgcc acagcagcag tatcagcagc cacaacagca ggttgcgccg cagccacaat
    18961 atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg caacaaccag
    19021 ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcaccgcag ccgcagtatc
    19081 agcagccaca acagccagtt gcgccacagc cgcaggatac cctgcttcat ccgctgttga
    19141 tgcgtaatgg cgacagccgt ccgttgcaca aaccgacgac gccactgcct tctctggatt
    19201 tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt gaacaaatgg
    19261 cgcgtctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtc gttaattact
    19321 ctccggggcc agttatcact cgctttgaac ttaacctggc tcctggcgta aaagcggcgc
    19381 gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg gtgcgtgtcg
    19441 ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accaaataaa aaacgacaaa
    19501 ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg tcgccattaa
    19561 ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg gcgaaaatgc
    19621 cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg aacgcgatga
    19681 tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg atcgacccga
    19741 aaatgctgga gctttcggtt tatgaaggca ttccgcacct gttaacggaa gtcgttactg
    19801 atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag cgtcggtata
    19861 aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa attgctgaag
    19921 ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac agtatggatg
    19981 cccagcatcc ggtgctgaaa aaagaaccat acattgtcgt gttggttgac gaatttgccg
    20041 acctgatgat gacggtaggt aaaaaagtgg aagagctgat agctcgtctg gcgcaaaaag
    20101 cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt gatgttatta
    20161 ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc agtaagattg
    20221 actcacgtac cattcttgat caggctggcg cggaatcact gctgggtatg ggggatatgc
    20281 tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt gttcgcgatc
    20341 aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag tatgttgatg
    20401 gcatcacctc cgacagcgaa agcgaaggtg gtgcgggtgg tttcgatggc gctgaagaac
    20461 tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa gcgtcaattt
    20521 ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt atcgaacaga
    20581 tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa gtgctggccc
    20641 caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc gcatccggca
    20701 ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt agcctggaat
    20761 agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat gatgaaaaaa
    20821 attgctatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg ggctgatgcc
    20881 gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag cttcacacaa
    20941 aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct gtgggtgaaa
    21001 cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct ggtttctgac
    21061 ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac ctggctgaaa
    21121 gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag cgactggcag
    21181 cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc cagcaatggc
    21241 aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca gtttagcgcg
    21301 gtggagcagg acgatcagcg cagcagttat cagctgaaat cccagcaaaa tggggctgtg
    21361 gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga tcaacgtaag
    21421 tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt ttcaacctct
    21481 ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggtcagcaac atttgctggc
    21541 tgcggggaag ccgttgccgc gcgctatcga agccgggcat ttgcattcta tgatcctctg
    21601 ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct atgcgaacgc
    21661 tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg aggcgatcga
    21721 gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg acgaagttca
    21781 ccgtttcaac aaaagccagc aggatgcatt tctgccacat attgaagacg gcaccatcac
    21841 ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac tgctttcccg
    21901 tgcccgtgtt tatctgttga aatccctgag tacagaggat attgagcaag tactaactca
    21961 ggcgatggaa gacaaaaccc gtggctatgg tggtcaggat attgttctgc cagatgatac
    22021 acgacgtgcc attgcagaac tggtgaatgg cgacgcgcgc cgggcgttaa atacgctgga
    22081 aatgatggcg gatatggccg aagtctatga tagcggtaag cgggtcctga agcctgaatt
    22141 actgaccgaa atcgccggtg aacgtagcgc ccgctttgat aacaaaggcg atcgctttta
    22201 cgatctaatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg cggcgctgta
    22261 ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc gtcgctgtct
    22321 ggcgattgcg tctgaagacg tcggtaatgc cgatccacgg gcgatgcagg tggcaattgc
    22381 ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca ttgctcaggc
    22441 gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt ttaaagccgc
    22501 gctggccgat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc gtaatgcgcc
    22561 gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttatgctc atgatgaagc
    22621 aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa cacgctatta
    22681 tttcccgaca aacaggggcc ttgaaggcaa gattggtgaa aagctcgcct ggctggctga
    22741 acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg gtaatgttgt
    22801 tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg ataagcacag
    22861 gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg ctgaaaaact
    22921 ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag agcgtcgtaa
    22981 agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat cgaaatccat
    23041 tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag tgaacaaact
    23101 gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg aaattcgcga
    23161 tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta aagacgaaaa
    23221 tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg aagttcgtga
    23281 ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag ttaagctgac
    23341 tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg cactgtcgca
    23401 gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg ttccgtacct
    23461 ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg gcgatctgtt
    23521 ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga tcccaacggc
    23581 agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg atctgccaat
    23641 taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg gtcgtgacac
    23701 ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga tcgtgcgccc
    23761 agaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag tcctgcagtt
    23821 gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct ttggcgcttg
    23881 caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg agatctcttc
    23941 ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc gcagcaagtc
    24001 ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg ttggtcgtac
    24061 gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag taccagaagt
    24121 tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt ttctgaatct
    24181 aaaaagcgcc tttagggcgc tttttttgtc tccctttgat accgaacaat aattactcct
    24241 cacttacacg taatactact ttcgagtgaa aatctaccta tctctttgat tttcaaatta
    24301 ttctatgtat acaagcctat atagcgagct gctatagaaa taattacaca atacggtttg
    24361 ttactggaat caatcgtgat caagcttgag tgagccatta tgaaaacgaa aatccctgat
    24421 gcggtattgg ctgctgagga gagtcgccgt ggtttggtaa aaacgacagc gatcggcggc
    24481 ctggcaatgg ccagcagcgc attaacatta ccttttagtc ggattgcgca cgctgtcgat
    24541 agcgccattc caacaaaatc agacgaaaag gttatctgga gcgcctgtac agttaactgt
    24601 ggtagtcgct gcccgctacg tatgcacgtc gtggacggtg aaatcaaata tgtcgaaacg
    24661 acaataccgg cgatgacaat tacgacggcc tacactaggt tcgcgcctgc ctgcgtgggc
    24721 gttccatgcg tcaccgtgtc tacaatccgg accgcctgaa atatccgatg aaacgagtcg
    24781 gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac gacatcatcg
    24841 cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat ctgaactatg
    24901 gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat accctggtcg
    24961 cgcgcctgat gaactgctgc ggcggttatc ttaaccatta cggtgactac tcttccgcgc
    25021 aaattgcgga aggtttgaac catacctacg gcggctgggc agatggtaac agcccgtcgg
    25081 atatcgaaaa cagtaaattg gtagtgctgt ttggtaataa cccaggcgaa acgcgaatga
    25141 gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat gctcgcatga
    25201 tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag tggatcccta
    25261 ttcgtccagg aacagatgcc gcactggtta acggtctggc gtacgtcatg atcactaaaa
    25321 acctggtgga tcaggcattc ctcgataaat attgcgttgg ctatgacgag aaaaccctgc
    25381 ccgccagtgc gccgaaaaat ggtcattata aagcttatat tctgggtgaa gggccagatg
    25441 gcgtggctaa aacaccggaa tgggcctcgc aaatcaccgg tgttccggca gacaaaatca
    25501 tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag ggatggggcc
    25561 cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga
    25621 ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca tacagcttac
    25681 cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg atgtttatgt
    25741 ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt gtgcgcggga
    25801 aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac tgcctgatta
    25861 accagcattc tgaaatcaac cgtacccatg aaatcctcca ggatgataag aagtgcgagc
    25921 tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac atcctgctgc
    25981 ctgactgcac cgcttccgaa cagatggact ttgcactgga tgcatcctgc gggaatatgt
    26041 cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag accatctatg
    26101 aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact gaaggccgta
    26161 cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt cctgaactgc
    26221 caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca caagggcatc
    26281 acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact acgccatcgg
    26341 gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg gaattgccag
    26401 aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt tatcaggatc
    26461 cgctgaacaa acagtatccg ctgcagctta caggtttcca ctataaatct cgcgttcact
    26521 caacttacgg caacgttgat gtgctgaaag cggcttgccg tcaggaaatg tggatcaacc
    26581 cgcttgatgc ccaaaaacgc ggtatccaca acggcgataa agtcaggatc tttaacgatc
    26641 gtggtgaggt tcatattgag gcgaaagtga cgccacgaat gatgccgggc gtggtcgcac
    26701 tgggtgaagg cgcctggtat gacccggatg caaaacgtgt cgataagggc ggttgtatca
    26761 acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca catacaaacc
    26821 ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg attttttatt
    26881 gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga ctacaaagat
    26941 ttgacgccag aagtcagttt ccgccgcatt tatgaatatg ctggcggcga ctggcaggaa
    27001 gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc gtgcaaccac
    27061 tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa acgcgaagat
    27121 ggttttgttg tggtcgatga agatgtatgc attggctgcc gttactgtca tatggcttgc
    27181 ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg cgatggttgt
    27241 tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgccc gctgcgggcg
    27301 ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc ggccgttgcg
    27361 ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa tgccaatagc
    27421 cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg agatgggaag
    27481 tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg tggcgggtgg
    27541 ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag cccagcagcg
    27601 tgttatcgcc tgcatgtttg gcttatgggt actgatgggc attggcttta tcgcctctat
    27661 gctccatctt ggttcaccga tgcgcgcctt taactcgctc aaccgggtag gggcttcagc
    27721 actcagtaac gaaatcgcca gcggttcgat cttttttgct gtaggcggca tcggctggtt
    27781 gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctatggctgg tggtgacgat
    27841 ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca ttgataccgt
    27901 tccgacctgg tacagcatct ggacgccgat gggctgggcg atgcgtctgc tgccagccat
    27961 ttctgtactg gcactggtgg tgagtggcgt gatgtcagtg atgcagggca cagagctggc
    28021 aactattcat agttctgtgc agcaggccgc agcgctggtg ccggactatg gtgcgctgat
    28081 gtcctggcgg atcgtgcttt tggccgttgc cctgtgcttg tggattgcac cacagctaaa
    28141 aggttatcag cctgcggttc cgctactatc cgtctcattc attctgcttc tggcagggga
    28201 attaatcggt cgcggcgtat tctatggttt gcatatgacc gtggggatgg ccgtcgcaag
    28261 ctaatcagta acaaccgggg tttcggcccc ggttctcttt tatttctgct tcgttaacgt
    28321 gtcataactg gtcatcaaat tacgataatc aggaatatgg ttagagaaca acgtcgccag
    28381 tccttcaata tcattacgcc agtcacggtg cagctcgcaa gccacgccga accatgtcat
    28441 caattgcgca ccagcttgcg acatgcgatc ccacgccgaa tgccgggtaa tttcattaaa
    28501 ggtccctgat gcatcagtca cgacaaagac atcaaaccct tcttcaatgg ctgaaagcgc
    28561 cgggaatgcc acgcaaactt cggttaccac accggcaata attaactgtt ttttacctgt
    28621 cgctttgact gcttttacaa aatcttcgtt atcccaggcg ttaatattgc ccgggcgagc
    28681 aatgtaaggt gcatcgggga attgtgcttt cagttctaga actaatgggc cgttaggacc
    28741 ggtttcaaaa ctggtggtaa gaatagttgg taagttgaaa tacttagcca ggtcacccag
    28801 cgccagcacg ttatttttaa acttatcggg ttcgatatcc cgtacaaggg aaagtaaacc
    28861 agcctggtga tcaacaagca aaacggcagc atcattttta tcaagacgaa catacggttt
    28921 ggtcatcctc ttctcctttc gagagtcagt ggtacgtctg aaatcggtca gacgcgatgc
    28981 attgctctga aagcatagac gggaaatatg agtttgctgt gaccatgaaa tttttcgact
    29041 gaacgaagcg tatgaaattt gtgttagatc aataaaaaca atcagatgag attaatcatc
    29101 tcttcagcat tagcgttatg aatgagtaat ataaatcgat aataccgccg ctgcgatatg
    29161 cgcgtatttt cacccattga caatgttttt ggcggtggca tgatgcgcat gaaatttgaa
    29221 cttcctcacg gttttaattc atgtccacgt atacccggcc tgtcatgctt ttgctgtctg
    29281 gcctgctttt gttgactctg gcgatcgcgg tgttaaatac actcgtgccg ctttggctcg
    29341 cccaggaaca catgtccaca tggcaggtag gcgttgtcag ctcatcctat tttaccggca
    29401 accttgtcgg tacattgctg acagggtatg tcattaaacg cattggcttt aaccgcagct
    29461 attatctgtc ctccttcatt tttgctgctg gctgtgccgg ccttggcctg atgattggtt
    29521 tctggagctg gttggcgtgg cgttttgtcg cgggcgtcgg ctgtgccatg atttgggtgg
    29581 ttgttgagag cgcgctgatg tgcagtggga cgtcacgtaa ccgtgggcgt ttgcttgctg
    29641 cgtatatgat ggtttattac gtgggaacgt ttttaggcca gttactggtc agcaaagtct
    29701 caaccgagct gatgagcgta ttgccgtggg ttacaggttt gacattggca gggatcttac
    29761 cgctgttgtt tacgcgtgtc ctgaatcagc aggctgaaaa ccatgattcg acgtcaatta
    29821 cggcaatgct aaaactccgt caggcgcggc ttggcgtgaa tggctgcatt atctcaggaa
    29881 tcgttctggg atctctatat ggcctgatgc ctctgtacct caatcacaaa ggggtgagca
    29941 atgccagtat tggtttctgg atggcggtac tggtcagtgc gggtatcctc ggacaatggc
    30001 cgattggacg tctggcggat aagtttggtc gactgctggt tttgcgtgtt caggtctttg
    30061 tcgtcattct cggcagtatt gcgatgctta gccaggcggc gatggcccca gcgttattca
    30121 tcctcggtgc cgctggcttt acgctatatc cggtggcgat ggcctgggct tgtgagaaag
    30181 ttgaacatca tca
//
LOCUS       DAJTYH010000042        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTYH010000042 DAJTYH010000000
VERSION     DAJTYH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO609_001880"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO609_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6633315.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO609_001881"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO609_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6633316.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO609_001882"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO609_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6633317.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO609_001883"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO609_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6633318.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO609_001884"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO609_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6633319.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO609_001885"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO609_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6633320.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO609_001886"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO609_001886"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6633321.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO609_001887"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO609_001887"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6633322.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO609_001888"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO609_001888"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6633323.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO609_001889"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO609_001889"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6633324.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO609_001890"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO609_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6633325.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO609_001891"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO609_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6633326.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO609_001892"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO609_001892"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6633327.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO609_001893"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO609_001893"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6633328.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO609_001894"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO609_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6633329.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO609_001895"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO609_001895"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6633330.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO609_001896"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO609_001896"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6633331.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO609_001897"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO609_001897"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6633332.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO609_001898"
     CDS             21622..22212
                     /locus_tag="OO609_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6633333.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO609_001899"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO609_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6633334.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO609_001900"
     CDS             22684..23379
                     /locus_tag="OO609_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6633335.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO609_001901"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO609_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6633336.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO609_001902"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO609_001902"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6633337.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO609_001903"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO609_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6633338.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO609_001904"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO609_001904"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6633339.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO609_001905"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO609_001905"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6633340.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7027 a         7824 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgccgcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTYH010000043        29795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTYH010000043 DAJTYH010000000
VERSION     DAJTYH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..511)
                     /gene="wcaD"
                     /locus_tag="OO609_001906"
     CDS             complement(<1..511)
                     /gene="wcaD"
                     /locus_tag="OO609_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6633341.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRT"
     gene            complement(486..1703)
                     /gene="wcaC"
                     /locus_tag="OO609_001907"
     CDS             complement(486..1703)
                     /gene="wcaC"
                     /locus_tag="OO609_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6633342.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1700..2188)
                     /gene="wcaB"
                     /locus_tag="OO609_001908"
     CDS             complement(1700..2188)
                     /gene="wcaB"
                     /locus_tag="OO609_001908"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6633343.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2191..3030)
                     /gene="wcaA"
                     /locus_tag="OO609_001909"
                     /pseudo
     CDS             complement(2191..3030)
                     /gene="wcaA"
                     /locus_tag="OO609_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3123..5285)
                     /gene="wzc"
                     /locus_tag="OO609_001910"
     CDS             complement(3123..5285)
                     /gene="wzc"
                     /locus_tag="OO609_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6633344.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5288..5731)
                     /gene="wzb"
                     /locus_tag="OO609_001911"
     CDS             complement(5288..5731)
                     /gene="wzb"
                     /locus_tag="OO609_001911"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6633345.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5737..6876)
                     /gene="wza"
                     /locus_tag="OO609_001912"
     CDS             complement(5737..6876)
                     /gene="wza"
                     /locus_tag="OO609_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6633346.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7185..7334
                     /locus_tag="OO609_001913"
     CDS             7185..7334
                     /locus_tag="OO609_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633347.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7535..9118
                     /locus_tag="OO609_001914"
     CDS             7535..9118
                     /locus_tag="OO609_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6633348.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9570..11423)
                     /gene="asmA"
                     /locus_tag="OO609_001915"
     CDS             complement(9570..11423)
                     /gene="asmA"
                     /locus_tag="OO609_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6633349.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11445..12026)
                     /gene="dcd"
                     /locus_tag="OO609_001916"
     CDS             complement(11445..12026)
                     /gene="dcd"
                     /locus_tag="OO609_001916"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6633350.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12118..12759)
                     /gene="udk"
                     /locus_tag="OO609_001917"
     CDS             complement(12118..12759)
                     /gene="udk"
                     /locus_tag="OO609_001917"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6633351.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13077..13319
                     /locus_tag="OO609_001918"
                     /pseudo
     CDS             13077..13319
                     /locus_tag="OO609_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13318..14259
                     /gene="mdtA"
                     /locus_tag="OO609_001919"
                     /pseudo
     CDS             13318..14259
                     /gene="mdtA"
                     /locus_tag="OO609_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14259..17381
                     /gene="mdtB"
                     /locus_tag="OO609_001920"
                     /pseudo
     CDS             14259..17381
                     /gene="mdtB"
                     /locus_tag="OO609_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17382..20447
                     /gene="mdtC"
                     /locus_tag="OO609_001921"
     CDS             17382..20447
                     /gene="mdtC"
                     /locus_tag="OO609_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6633352.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20448..21863
                     /locus_tag="OO609_001922"
     CDS             20448..21863
                     /locus_tag="OO609_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633353.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            21860..23263
                     /gene="baeS"
                     /locus_tag="OO609_001923"
     CDS             21860..23263
                     /gene="baeS"
                     /locus_tag="OO609_001923"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6633354.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23260..23982
                     /gene="baeR"
                     /locus_tag="OO609_001924"
     CDS             23260..23982
                     /gene="baeR"
                     /locus_tag="OO609_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6633355.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24173..24505
                     /locus_tag="OO609_001925"
     CDS             24173..24505
                     /locus_tag="OO609_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6633356.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24652..26013
                     /gene="yegQ"
                     /locus_tag="OO609_001926"
     CDS             24652..26013
                     /gene="yegQ"
                     /locus_tag="OO609_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6633357.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26355..26672)
                     /locus_tag="OO609_001927"
     CDS             complement(26355..26672)
                     /locus_tag="OO609_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633358.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27078..27977
                     /gene="yegS"
                     /locus_tag="OO609_001928"
     CDS             27078..27977
                     /gene="yegS"
                     /locus_tag="OO609_001928"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6633359.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28059..28832)
                     /locus_tag="OO609_001929"
     CDS             complement(28059..28832)
                     /locus_tag="OO609_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6633360.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(28938..>29795)
                     /gene="gatD"
                     /locus_tag="OO609_001930"
     CDS             complement(28938..>29795)
                     /gene="gatD"
                     /locus_tag="OO609_001930"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6633361.1"
                     /translation="SGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAK
                     YDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKN
                     VIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQ
                     SVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRK
                     ELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIARNA
                     MQGKVLLIP"
BASE COUNT         6847 a         7938 c         7736 g         7274 t
ORIGIN      
        1 tcgtgcgctt gccgccgaat ttaatgaaat tcagcacata gctgttgctg taaatcagat
       61 atttcgaaat cgactccata atactgctgc caccggtgag gataatttgc gccatctcca
      121 ccgccgccag cgccaccacc agtccgacca ccagataaaa gaaacgtaat atcttacgat
      181 ggttgtgtgg cgaaattgtt ttaaaacgaa tactccacac catgccaata atgatcacaa
      241 tatagacaaa cagcatagtt gaagtgacgt atttaccggc atccaacgac tgaccaaaca
      301 ggtagttaaa cgccgtgagt cccgcgccaa tccctaaggc aatcatcaat tttttaatgc
      361 tgatgcgttc taaaaacagc agtaacaaga caggtaaaaa agtgacgatg gtgatgggga
      421 aactttcgcc aagctgggcg attttgacgt taaccagcag gtagatcagt ggcagcagca
      481 ggtagctaca gattctgata gaagttgaca tactcctcca gcatctgttg tccactgtag
      541 gcggcgcgac tgcgttggct gaactcagcc agcgtggtac caaatatcgc ctgcgcgatt
      601 tccggtttgc ttaactgtac cagttgcagc acctcttctt cactgacggt tttaccaccg
      661 gatttttgca gcacttcccg cgccgcatcg ctatgggtgg caatcaccgg cacgccaatc
      721 gatagcgcct cacacaaaat cagcgggtag ttatcgacac gagaactgaa caccagcgca
      781 tccatctgat tgagcgcgct catcaacttg cgcttgtcag tttcaaagcc gtgattaacc
      841 acgttgccag cggtgaacgg cgagaactta ccaaaggtat gcagttcgat tttgtcgccc
      901 agcgccatca tctcacgcac cagttgctgg ttagttttgc cgtcgtaacg caggtcatgc
      961 gccaccactg cgattttcgg cttgccctga gtttcgcgca ccggaggcag atctgccaga
     1021 atcgcttcgg ttgccatatc aatgccatta ttgataatcc ggcaacgccc tggcccgtac
     1081 agactattga aagcgtcagc cacatgctgg ctgggggaaa taaactgaca gcccagcgcc
     1141 agcatctcgc ggaataactg gcgtttgccc gccaccagct ggtgcgcgcg atcaatcttc
     1201 accggcggat agttatttaa ggtcgggcat ttctggcagc ccgttttcca gccttcgcaa
     1261 ccgtcggtaa aggcgcagcg tccggttaca ctccagtggt cgtgcagcgt ccagaccagg
     1321 gtgacatccg gtttatggtt tttcactttt tcgcaaaagc gcaccacgct gttaagattc
     1381 aaccagtagc tgtgcagcac atgaaaatgc aggaccaccg gacccggtgt gcgagtaatg
     1441 gtgcgatata actcattgaa attgccgaac agatcgcgat taaacagacg aaacaacgca
     1501 atgttcgcca tcgcggtcat ccgcggcgta tgtttgatga cctgcggata gttctgatgg
     1561 ctgacgctct ctttgccgcc tttgccgtag ccgtagacaa aatgtgacgc cagcccctgt
     1621 tgcagcgcac gctggtggag atctaacgcc acacctgctg ccccgccttc cgccagtcgc
     1681 acattaaatt gcagaatatt catttaatca ccttcactcg cgctttttct cccaccacca
     1741 gcgcgttgtc cgggacagag tcgagcacca cgctgcccgc acccacggtg acgttgttac
     1801 caagcgtgat atcaccaaga ataatgacgt tggcaccgag ttcgacgccg ttgccaatgt
     1861 gtggacatgc catgttatcg gcaccccgat tgccgatagt gacgccgtgg cgaatggtga
     1921 aatcatcccc cgccaccacg tttttattga tcactacggc gtaaccgtga tggatagtaa
     1981 agcggcggcc aatggtcgcg gcggcctgga tttcataacc gaaaaagcat tcggtgataa
     2041 tgcgatacag caccagcagc ggggccgccc acagattgtt gaggacgttc tttttgcgcc
     2101 acaccgaaca aaaatgagcg acgcgatagg caagtaccat gcagcacggg cgtaaactcc
     2161 agctgttggc gcgcagatct tccagcatct ttagcgcccc cgaataccat cagccagacg
     2221 tttgccgtta cgcaccgaca gcagcgtcaa caaggtgcgc caggtcatac gtttattgcg
     2281 gatctggtag agggtaaaca gctgatattt tttactggcg cggtcgaatt tgtctttgtg
     2341 cttgcggtaa aagtggaagt atccggagaa ttttttcggt gacgaggtga tctgcatttc
     2401 gccgtgattg atatgcagga tctgcgtcgc ctcttccact ttccacggtt cgccgtactc
     2461 caccaccatc cgcaggaata tgtcgtaatc ctgtgctgct ttcagttcgg tatcgaacaa
     2521 acactctttg aaacgccatg cccaggtaaa gacctgattg ccaatgatat tacgtttata
     2581 gaacaggcgg cgtgagtacg gcgatttggg atacagtggt aagctcgccg gttgggaata
     2641 aacttctccc tagcaaacgt agtcgttagc gtacaaaaag gcgtgtgtga ccagttgctg
     2701 tttatgggcg aggaagacgc tcaggcggtt gggcgtccat tcatcatcat catcgatccc
     2761 ggtgatgtat tccccttgcg ccagcataat cgcctggtta cgcaccgcgc acgccccgct
     2821 gttaatgtcg ttgtggatgt aagtgatacg cggatcgttg agggcagtga cgtactgttg
     2881 cagttgttcc caggaggtgg agcaatcatc cacgatgatc atctcccagt tgctgtagtc
     2941 ctggcgcagg accgatttta tcgcccgaat cgccagttgt tggcggttcc aggttggcat
     3001 atagattgag atcagcggat tgtctttcat aggttgttct cccggcttat gagctagttt
     3061 gcttgccgga tgcggcgtga gcgccttatc cggcctacgg ggcggtgcga atgcaggcgt
     3121 cgttatttcg catccgactt atattcgtat tcgtaatagc cataatcctg atacgcgctg
     3181 gcgcggcgga agatggagtt cagaatcacc cctttcaccg gaataccgtt ttgctcaaag
     3241 cggctcagac tggtttccac ttctttcaat gtgttgaccg cataacgcgc caccattaac
     3301 gtggttccga catgacgacc aacaattgcc gcatcggtca ctgccagaat cggcggcgta
     3361 tcaatcaaca ccaggtcata gtttttactc gcccagttca ccagttcggc aaagcgttcg
     3421 ctcattaaca gttcagaagg atttggcggc acctgaccgc gcgggatcag gtcaaatttg
     3481 gcaatagagg tcggtttagc agcggtagta atatcgccct gaccaatcag aatctccgac
     3541 agcccattga cattattggt gcccaacagc tcgtgggtgt agcctttgcg catatcgcag
     3601 tcgatcaaca acacgcgttt attggtctgg ctgatcaccg ccgccaggtt ggcgcagaca
     3661 aaggttttac cgattgacgg gctaaccccg gtcatcatca acacattgtt ctgcgcctgc
     3721 atcatcgcga agtgcagact ggtacgcaga ctacggatgg cttcaatcgc cagatcggtt
     3781 ggattcccca ctgccagtag ctggctctgt ttatagcgtt taaccccttt gatggttttg
     3841 acgctatcgc gcgctttctg ccactccgac agcgggatgc tggcatagac gctgattccg
     3901 tgttcttcca gcacttgcgg gctttcgatg ccgcgattaa acaacgagcg cagcagcaca
     3961 cccacgatag agagcatcag gccaaggata atcgcgccga ggataatcag ccctttcttc
     4021 ggttttagca cgccaggctg ggtgattgcc gggtcaataa tgcgcacatc gccgacggtg
     4081 ctggcttcgg tgattttcag ctcctgctct ttattcagca gttgcatata gacctgctga
     4141 ccagactcga catcgcgggt cagacggaca atttcctgct ggtttttcgg catcgccgtt
     4201 acgcgaccgt taagtttggc tttctcttct tccagcgcct gacgtttctc cagcagcgtg
     4261 cggtacgccg ggtgaacttt ggtgtacagc ttggagattt ccgcctcttt aaaggtcagt
     4321 tcgttcaact gcgcgtcgat gttcaccatc gaatcgagca ccgctttcgc ttcaagcggc
     4381 agatcaacag aatctttatc ctgacggaag gcattcagtt tgttttcggc aacatcgagg
     4441 cggctacgta cttccggtaa ctgttgcgcg aggaaagcga ggcttttcga cgcttccgcc
     4501 gatttgcgct caatattttg ttcctgatag ttacgagcga tgctgttaag aatgtcgcgg
     4561 atctgttcgc gatcttcacc ggtataagtc aggctcagta cgcctgcgtc tttgccgttc
     4621 tccgttaccg tcaggctgtt ttgcagttgg ttgatcatcc ccaacgtgga gtatttggtg
     4681 acggtaaact cactgcccgg gcgggcgtga atggcttcaa ccatcagcgt gacgccttct
     4741 tttttcagca tctggcccgc ttgcccacgg gcgctaaagc cgccatcact gctcagggtg
     4801 tagtttttgt catccagcac attaagcgta aacacctgat ccgccatctc tttcgggcgg
     4861 ttaaaggtag tcactttcac cgtctcgttc tgacgcccca tcaggcgatc ccagcccgcc
     4921 ccgaaaatcg ggaacgtgtt tttgctcacc gcaatatcga ggtcgagatc gtccaccgtt
     4981 ttaccaagca ccagacgcga acgaatcaac tggatctcgg cgtccgatgc aggcggtttg
     5041 ttggctaatg ccgagccgat atcctgcacc agcgaattgc cgctgttttg ctcgatttgt
     5101 accagtgcgt cggcactata aatcggcgtg gcgaagaaag tgtaaaccac ggcacagagg
     5161 gcaaatacga cggtgatgcc aatcacccac cagcgcgctt caatgacggt gccgaccagg
     5221 cgaccaatat cgatttcatc actgcccgtt accggagcgg catgttgttt tactttttct
     5281 gtcattctta tacctgctct gcgttcaatg cctgcgccca ctggcgggca gaccgttcaa
     5341 gtaatgtgta caccgctgca aacgtttccc ggcttttgtg atacggatcg gggatttcac
     5401 attcgttatc ccagtgacca aacagcatca ctttgccgcg catctccggt gccatctcgc
     5461 ataagcgttc gatatggcgc ttatccatgg tcaaaatcag gtcatagttg cgacacaggc
     5521 ggcggctgat ttgtcgtgca cagtggcctt ccagagacag ttgatgttct gcggcgacgc
     5581 tgatagcggt aggatcagcg cccttaccga ccagcgcgcc gagtccagcg gactccactt
     5641 tcagttccgg gtgataacgt tgcagtaagc gttccgccat cggggaacgg caaatattgc
     5701 cgacacagac aactaagatg ttgttaaaca tgacgattac cagttatgaa tgtcgctggc
     5761 tgtatccgtc atgtaacgga caccgctaat agttggcagc aactgattga tcagacggtt
     5821 ccagcgggaa accggggcgg tggtgacata caccacgtca taaggttgca ggcggaattc
     5881 tgtcgccatc accagcgacg tcgcatcgga catatccagc tggtagatat tggcaatctt
     5941 gccgttacgc ccgccctcgc ctttcagcgg acgaatgaca aagatgccgc tggcgttgga
     6001 ggtggtcatg tcgatacctt cggcattggc cagagcttca gtcagggtca tgccgctaaa
     6061 gtccattttc agggtgctct gtttcttcac ttcacccatc acaaatactt tcagatcatc
     6121 attacgcggc acgtagagaa tatcgccggg ataaagcagt cggttctgat tgaggtcgcc
     6181 gttttgcatc agcgcctgta aagaaatgcg ttcttcacga ccattgtgtg ttagcaccac
     6241 gttgcgccag tcagcggtgt cggtcaggcc acctgcggcg ttgatggcgt cgagaatggt
     6301 cagtggtacg ttggtgatcg cctgttgacc ggatttattc acctgacctg agatataggc
     6361 cttttgtgag cggaaggcgg cgatattaac gtccacctgc gggtcagcga tgtacgtcgc
     6421 taagcgcccg gtaatatcac tgcggatttc agcgagcgtt ttcccgacta cgtgaacctt
     6481 gccgatatac gggtaaaaca tagtgccgtc aggctgtacc cagttgccgg tgtcgctgga
     6541 gctgcggtac tgaccagctg gcgtggtgag ttccgggtga tcccagacgg tgacattaag
     6601 aacgtccccc ggcccgacgc gatactggta attcgcgatc tcgctttcca gcgtcatatt
     6661 ggggcgcgct acattcgggc gtgggcgtaa ttggtcaatc aggcgcgggg tcagcggata
     6721 aacattcacc attttgtcga gatcgaaatc agcgtcctgc tgtttgatga cgtctttgcc
     6781 catcgtcgac atattgctgc ccggaagtac tgtgcaaccg cttatcaagg ttactgacac
     6841 caataatggc atcaatttca ttttggattt catcattgtt tatttatcac tttggcagag
     6901 taattatcct gtgcactatt aatagcaatg tcgccatgca catttacctt gcagttaatt
     6961 gaataaaaat ttaactggca tcagtcctaa aaaaattgat ttcatccgca ggctattgac
     7021 agaataattc agactggtct ttcaggcatc cagacacgct accgcccctg gctttttagc
     7081 taccaataca ctgattgagc ttaatttttc aaaccctctc tgcatacaat cgttgatgag
     7141 aaagggttat tgcggaaatt aacttccgaa tataaggtga cattatggta attgaatatt
     7201 ggctcgccaa taatgcagga ggaagtgtta cagctaacgg aatagcaggc aaggtaacaa
     7261 ttcggcaatt ggctattttt aagaattata ttaagtatca ttcaatatgg tttttaggag
     7321 tttctttagg ttgacaatat ttaatattgc gtctccacat gcgatatttc ttaaataatg
     7381 ttttattatt accgctttta attcagggat aatgtgaggt tattaccctc aaataatatg
     7441 agataaatag tgctgcaaca ttgcattttt gccccgattt atccatgatc gaattgtgac
     7501 atttgtcata caacgaatag gttttgtact tactatggaa tggattgccg atccgtctat
     7561 ctgggccggg ttaatcacgc tgattgtgat cgaactggtc ctcggcattg ataacctggt
     7621 ctttattgcc atcctcgccg aaaaactacc gccgaagcag cgcgaccgcg cacgggttac
     7681 cgggctgctg ctggcgatgt taatgcgcct gttactgcta gcgtcaatct cctggctggt
     7741 caccctgact caaccgctgt tcagcttccg ctcgtttacc tttagcgccc gcgacttaat
     7801 catgctgttt ggtggtttct tcctgttgtt caaagccacg atggagctga acgaacggct
     7861 ggaagggaaa gacagcaata atcccacaca acgcaaaggt gcgaagttct ggggcgtggt
     7921 gacgcaaatt gtggtactgg acgccatttt ctcactcgac tcagtgatta ccgccgtcgg
     7981 gatggtcgac catttactgg tcatgatggc cgccgtggtt atcgctatca gcctgatgtt
     8041 gatggccagc aagccgttaa cgcaatttgt taacagtcac ccgaccatcg ttattctctg
     8101 cttaagcttc ctgttgatga ttggctttag cctggtggca gaaggtttcg gcttcgtcat
     8161 tccgaaaggc tacctgtacg ctgccattgg tttctcggtg atgatcgagg cactcaatca
     8221 gttggctatc tttaaccggc gacgttttct ttccgctaat cagacgctac gccagcggac
     8281 taccgaagcg gtaatgcgcc tgcttagcgg gcaaaaagaa gatgcggaac tggacgccaa
     8341 aaccgcgtcc atgctggtgg atcatggaaa ccagcagatc tttaatccgc aggaacggcg
     8401 gatgattgag cgggtactta atcttaacca gcgtaccgta agcagcatta tgacgtcgcg
     8461 ccacgatatt gagcatatcg atctcaacgc gccggaagaa gaaatccgcc agttgctgga
     8521 gcgaaatcag catacgcggc tggtggttac cgacggtgat gacgcagaag atttgctcgg
     8581 tgttgttcac gttatcgacc tgctacaaca gtcactgcgc ggcgaaccgc tcaacctgcg
     8641 ggtgttgatt cgccagccgc tggtgttccc ggaaaccttg ccgttgttac cagccctgga
     8701 gcagttccgt aatgcccgca cgcactttgc ttttgtggtg gatgagtttg gctcggtgga
     8761 agggattgtg acattaagtg acgtcactga aaccattgcc ggtaacttac cgaacgaagt
     8821 ggaagagatc gacgcccgcc atgatattca gaagaatgcg gacggttcct ggacggcgaa
     8881 tggtcatatg ccgctggagg atctggtgca atatgtgccg ctgccgctgg atgaaaaacg
     8941 tgaatatcac accattgccg ggctgttgat ggagtatttg cagcgtattc caaaacctgg
     9001 cgaagaagtt caggtggggg attatttgct taaaacgttg caggtggaaa gccatcgcgt
     9061 gcagaaggtg cagattatac cgctgcgtaa ggatggcgag atggagtacg aggtgtgatg
     9121 gtatcggcca ttttacccaa tgctgcgtaa cgccttataa cttcagcaat gaagcgttgt
     9181 ctgtggtaaa cggacatcac agcgggggtg tgggtccggc tgtaaatcgc cgaatcgttt
     9241 cctgtaggct ggggcagccc acatggatgt gggctgaggg cgcgttttac agggatgtta
     9301 cctcgcgccc gtacccgata gccgaaagga ataagatgag ggaaccgcgc cagcggcgat
     9361 ttaccgccgg gagcccgggt tgccagggtg gtggcgattg agccaccctg gcacgttcac
     9421 cggcttgagc gttccatagt agcgaggaac atcaagtgaa cggaacagcc ttcgatctta
     9481 catattcaga atataatagt gccgccgcga acgcctgatg cgacgcttta cgcgtcttat
     9541 caggcctaca ggacaggtca cctggtaacc tacatcttct ccagcaactt cttcacatct
     9601 ttgccattgc gggaatcttt attccgctcg gcccagtcat tcaggcgacg tttcgcttcg
     9661 tcctgtagat gtttgcgcag taactgatcc acttgcaggc tgtaattgag ttgctgccag
     9721 ttgccataaa cccgcagcgg tactggcgtt tctttgagga aatcaatcag tttgctttcc
     9781 ccgttccagc cacccacgac ccgaatatca aactgggtgt cgcaggtttg atctgccaga
     9841 ttcaacatgc cttcccctgt cagcgccagc acaggcgatt gaccttgcat gtcgtttaac
     9901 gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc ggtcaagccg cgtcacgtta
     9961 tcgaagtttt cagcggcctt cacatcaccg ccattacgct ctaccgcttg ctgaatcatc
    10021 tgctggaagt tcatcccttc catacgtgtg tcggtcatct caacatgcgc ctgtccttgc
    10081 cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc agccagtgac
    10141 atttttccgg tcaacgaaat cggatagtta aacgccttca gaatggtacc aatctcaacg
    10201 ttctccagcc gtggctggaa gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc
    10261 ggcagtgaaa cctttccacc gttaagcttg ccctgcagtt gagtaatttc cagcaaaccc
    10321 gacttgttgg tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta
    10381 ctggcctgca acaaaatatc agctgtaaag ccttgcagtc cctgataggc cggttcatca
    10441 atacgcgaag aaatgaccgg gcgcggcaac gttgattggc tctgcccctg ctgcgcggca
    10501 ccgttttcac cattcgcggt ttcattaagc gggatgaggt tgtcaagatt cagttgcggg
    10561 aattgcagtc tcagctgcca ttccggtttc tctgtcagcg tgacctgtgc ttgtccgctc
    10621 agcgtactgt cattggcggt caaactaatt tggttaaatg aaaggcgttt atgtgactcc
    10681 tgccactggg actggaaact cccctgcccc tgaatacctt gttttggtaa atcggcaccc
    10741 tgcaactgcc agttaatttg ttcaatagcc gctgttaaat catgcggata atcagaagca
    10801 tctaccgtac cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac ccgcccggag
    10861 aactcaaatg agccacgatg ttggggatct tgttccattt gcaggcggat attgcggatt
    10921 gtcacctgct cgtcatcttc atgctggaac accagaacgc tgtccgccac cttaagactg
    10981 gatatatcaa acgaccatcc gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt
    11041 gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg gatcactgcc
    11101 ccttttagca tcacctgctt aacgctcagt tgatgactca gtagtggtaa aagcgccacg
    11161 tccagacgca tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc ggtgagagac
    11221 attcgcccgg agaggatact aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc
    11281 tgcaattgat aaccgctacg tgcagcaact tgcttgacca tatagtcgcg gaaatcattc
    11341 ggattcacca gcaacactaa cgcagataac ccggccacca gcacgaccaa gagtatcatc
    11401 agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc tgaattagtc tttatcgatt
    11461 cggctggcta ccgcgccctg ctggttgcga tatttcgcat cttcacggcg gttgtaaggt
    11521 cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac caattaacat gcccggacgc
    11581 agcgccagcg gcagcttacc ggagttgtag aactccagca caatgcaacc agaccagccc
    11641 ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa agaggaacgc
    11701 ccgtccagcc agcccaccag atcggctggc agcgtcaccg actccagtgt caccgccagc
    11761 gccagctctc ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc
    11821 atcacgcggt caagcgcggc gctcacttca tctttgggac cgctcagatc gataaacgct
    11881 gccgtgtgac cacggaaggt acgaaattta ttgcccaggc gtacatccac cgtcgcgccg
    11941 ttaatacgct ccactggcgg acgtgggttg atcgacaaac ggccttcatc aagccaggct
    12001 tcaatatctc ggtcacacag acgcatggca ttttctcctt tcgcacatca ctcccttaac
    12061 gccaattacg tcaagggcat accaacttat cactgaacgg tacacaattt atcaagctta
    12121 ttcaaagaac tgacttattt tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc
    12181 gcgcggcacg ataatgtccg catattgttt agaaggctca atgaattgca ggaacatcgg
    12241 gcgcacggtt ttttgatatt gcgccatcac ggaatccatt gaacgcccac gctcgttaac
    12301 gtcacgcttg atgcggcgca tcaggcagat atccagcggg gtatcaacga aaatggagaa
    12361 gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga gaatgatgac
    12421 cttcttcggc tcaaccgtca ccgtttcttt catacgcgta tgttcaacat agctgtaaac
    12481 cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca acaggctgtg
    12541 atccatcgcg ctgggatggt cgtagttggt cttaacgcgt tcttccatcg acagatggct
    12601 ttgatctttg taatagcagt cttcgggaat tacgccgatg tgttcatcac cgacttgctc
    12661 acgtaattca cgataaaggg tactggcaat aagactcttg ccggaagccg atgcgccagc
    12721 gataccgata atgacgcact gatgagactg atcagtcata tatttagcga cctgattaac
    12781 ctggatgtta ggaagggggc gacgaagcgc caaacgcggc aattataggg atttcatccg
    12841 tctgatacca gtcgaatagc gttgccgcgc gctcagagtt aattgttgac aaagaattcc
    12901 cggtggcaaa ttacgttgat cagttttatg taaggtaaaa aatgttatac gcagttgcgc
    12961 aaattatccg cctttacgtc actttatgag caattcgcat ataaaatgta aaacttttgt
    13021 actagcataa acacagaaac gaatactggc gacctggcct tgcggataaa gcggtaataa
    13081 gcaaacaatc acagcatgta ttaattgccc tgccccaccc gctgcttcac ctggtcagtt
    13141 taggtttagt ctcgtttatc tttacccttg tctcgcttga gctttcgcag tttggcaccc
    13201 aactcgcccc actgtggttc ccgacgtcca tcatgatggt ggcgttttat cgccatgccg
    13261 ggcgcatgtg gccgggaatt gcgctgagct gttcgctggg aaatatcgcc gcatccattc
    13321 caggaaggcc agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc
    13381 aaagttgcat tagcacaaac ccagggccaa ctggcaaaag ataaagccac gcttgctaac
    13441 gcccgccgtg atctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag
    13501 gagctggatg cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa
    13561 gcaagcgtcg ccagcgcgca gttgcaactc gactggagcc gcatcaccgc accagtcgat
    13621 ggtcgcgttg gtctcaagca agttgatgtt ggtaaccaaa tctccagtgg tgataccacc
    13681 ggaattgtgg tgatcaccca gacgcatcct atcgatttag tctttaccct gccggaaagc
    13741 gatatcgcta ctgtagtgca ggcgcaaaaa gccggaaaac cgctgatggt agaagcctgg
    13801 gatcgcacca attcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc
    13861 gatgccacaa ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg
    13921 tttcccaatc agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg
    13981 atcccaacag ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc
    14041 gaaaacaagg tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg
    14101 atccgtgcag gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc
    14161 gaaggggcga aagtggaagt ggtggaagcc cagagcgcca ccactccgga agagaaagcc
    14221 accagccgcg aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca
    14281 gcacaggcgg cccgtcgcgc ctgtttatta tgcgtcctgt cgccaccacg ctgctgatgg
    14341 tggcgatctt actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg
    14401 aagtggacta tccgaccatt caggtggtca cgctctaccc aggtgccagc ccggatgtca
    14461 tgacctctgc cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac
    14521 agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat
    14581 taccgctcaa tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt
    14641 tgccgagcga tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga
    14701 tcatgacgct cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg
    14761 aaacccgcgt cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg acgctttccg
    14821 gcggtcagcg tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc
    14881 tgaccagcga aaccgtgcgc accgccatta ccggcgctaa cgttaactcg gcaaaaggta
    14941 gcctcgacgg cccttcccgt gcggtcacgc tttccgcgaa cgaccagatg caatccgccg
    15001 aagagtatcg ccagctaatc atcgcctacc agaacggcgc gccaattcgt ctgggcgatg
    15061 tcgcaactgt agagcaaggt gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac
    15121 aggccattgt gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca
    15181 gcattcggca gatgctgcca cagctcactg agagtctgcc gaaatcggtg aaggtgacag
    15241 tgctttccga tcgcaccacc aatatccgcg catccgtcga tgatactcag tttgaattga
    15301 tgatggctat cgcgctggta gtcatgatta tctacctgtt tttgcgcaat attccggcga
    15361 ccatcattcc cggtgttgct gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt
    15421 ttctcgattt ttcaatcaat aacctgacac tgatggcttt aactatcgcc accggattcg
    15481 tggtcgatga cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa
    15541 aaccgttggc ggcggcgctc aagggcgcag gtgaaatcgg ctttaccatt atctcgctga
    15601 ccttctcact gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac
    15661 tgttccgcga atttgctatt accctggcgg tagcgatttt gatctcagcg gtggtgtcgc
    15721 tgaccctgac accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga
    15781 acagcttctc ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg
    15841 gactggcgaa agtgctgaat catccgtggc tgaccttaag cgtggcactc agcacgctgc
    15901 tgcttagcgt gctgctgtgg gtgttcattc cgaaaggttt cttcccggta caggacaacg
    15961 gcattattca gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc
    16021 gacaacgcca ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat
    16081 ttgttggcgt tgatggcact aacccgtcgc tgaatagtgc acgtttacaa atcaacctca
    16141 aaccgttgga tgaacgtgat gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg
    16201 tagataaagt gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg accatcgata
    16261 ctcaggtcag ccgcacccag taccagttta ccttgcaggc cacgtcactg gatgcgctca
    16321 gtacctgggt gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct
    16381 ccagcgactg gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca
    16441 gccgtctggg gatcagcatg gcggacgtcg ataacgccct gtacaacgcg tttggtcagc
    16501 gactgatttc cactatttat actcaggcta accagtatcg cgtggtgctg gagcacaata
    16561 ccgaaaatac cccaggcctc gcggcgctgg ataccattcg cctgaccagc agcgacggtg
    16621 gcgtggtgcc gttaagctca attgccaaaa ttgagcagcg ttttgcgccg ctttccatca
    16681 accatctgga tcagttcccg gtaacaacca tctcctttaa cgtgccggat aactattcgc
    16741 tgggcgatgc ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata
    16801 tcaccacgca attctagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct
    16861 ggctgattgt cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct
    16921 ttattcaccc gatcaccatt ctctcgacgc tacccaccgc aggggtcggc gcgctgctgg
    16981 cattaatgat tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga
    17041 tcggtattgt gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg
    17101 agcaaggcat gtcgccgcgc gatgcaatct accaggcttg tctgttgcgt tttcgtccga
    17161 tcctgatgac caccctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg
    17221 tcggcgcgga actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc
    17281 aggtgctgac gctgttcacc acgccggtga tttatctgct gttcgaccgc ctggcattgt
    17341 ggaccaaaag tcgctttgct cgccatgaag aggaggcgta agtgaagttt tttgccctct
    17401 tcatttaccg cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac
    17461 tgggtttccg tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg
    17521 tcagcgcctc gctgcccggt gcgtcgccgg agacaatggc gtcttccgtt gccacgccgc
    17581 tggagcgctc acttgggcgc attgccgggg ttagtgaaat gacctccagc agttcgctcg
    17641 gcagcacgcg cattattttg cagtttgatt ttgaccggga tatcaacggc gcagcgcgtg
    17701 atgtgcaggc ggcgatcaac gctgcacaaa gtttgctgcc cagcgggatg cccagccgcc
    17761 cgacctatcg caaagcgaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg
    17821 atacttattc gcagggtgaa ctgtacgatt tcgcctcgac gcagctggct ccgacgattt
    17881 cgcaaatcga cggtgtcggt gatgtcgatg tcggtggcag ctcgctgccc gccgtgcgcg
    17941 tcgggctgac cccgcaggcg ctgtttaatc agggcgtgtc gctggacgac gtacgcactg
    18001 ccatcagcaa tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct
    18061 ggcagatcca gaccaacgat gaactgaaaa ccgccgctga atatcagccg ctgattattc
    18121 actacaacaa cggcggcgcg gttcgtctgg gcgatgtggc gacggtgact gactcagtgc
    18181 aggatgtgcg caacgccggg atgaccaacg ccaaaccggc tattttactg atgatccgca
    18241 aactgccgga agccaatatt atccagacgg tagacagcat ccgggcaaaa ttaccagagt
    18301 tgcaggagac cattccggcg gcgattgatc tgcaaattgc ccaggatcgc tcccccacca
    18361 ttcgcgcctc gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc
    18421 tggtggtctt tttattcctg cgctcgggtc gcgccactat tattcccgcc gttgcggtgc
    18481 cggtttcgct gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataatc
    18541 tttcgctgat ggcgctcacc attgctactg gtttcgtggt ggatgacgcc attgtggtgc
    18601 tggaaaacat tgcccgtcat ctggaagcgg ggatgaaacc actacaggcc gcgctgcaag
    18661 gtactcgcga agtcggcttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc
    18721 tgccgctgct gttgatgggc ggattgccgg gccgactgtt acgcgagttt gccgtgacgc
    18781 tttctgtcgc cattggtatt tcgttgctgg tttccctgac gctaacgcca atgatgtgcg
    18841 gctggatgct aaaagccagc aagccgcgtg agcaaaagcg actgcgtggt tttggccgca
    18901 tgttggtagc cctgcaacaa ggctacggca agtcgctgaa atgggtgctc aatcataccc
    18961 gtctggtggg cgtggtgctg cttggcacca ttgcgctgaa tatctcgatc ccgaaaacct
    19021 tcttcccgga gcaggacacc ggcgtactga tgggtgggat acaggcggat cagagtattt
    19081 cgtttcaggc gatgcgcggc aaattgcagg atttcatgaa aattatccgt gacgatccgg
    19141 cagtggataa tgtcaccggc tttaccggcg gttcacgagt taacagtggg atgatgttta
    19201 tcaccctcaa gccacgcgac gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc
    19261 gcgtgaagct ggcgaaagaa ccgggggcga atctgttcct gatggcggta caagatattc
    19321 gcgttggtgg gcgtcagtcg aacgccagct accagtacac gttgttatcc gacgacctgg
    19381 cggcactgcg cgaatgggag ccgaaaatcc gcaaaaaact ggcgacgttg ccggaactgg
    19441 cggacgtgaa ctccgatcag caggataacg gcgcggagat gaatctggtt tacgaccgcg
    19501 acaccatggc acggctggga atcgacgtac aggccgccaa cagtctgtta aataacgcct
    19561 tcggtcagcg gcaaatctcg accatttacc agccgatgaa ccagtataaa gtggtgatgg
    19621 aagtggatcc gcgctatacc caggacatca gtgcgctgga aaaaatgttc gttatcaata
    19681 acgaaggcaa agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg aatgccccac
    19741 tatcggtgaa tcatcaggga ttatcggcgg cctcgaccat ctcgttcaac ctgccgaccg
    19801 gaaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag cttggtgtgc
    19861 cttcgacggt gcgcggcagt tttgccggca cggcgcaggt tttccaggag acgatgaact
    19921 cgcaggtgat cctgattatc gccgccatcg ccacggtgta tatcgtgctg ggtatccttt
    19981 acgagagtta cgtacatccg ctgacgattc tctccaccct gccctcggcg ggcgttggag
    20041 cgctgttggc gctggagctg ttcaatgccc cgttcagcct aatcgccctg atagggatca
    20101 tgctattaat cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt gcgcttgaag
    20161 cccaacggca cggtaacctg acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt
    20221 ttcgcccgat tatgatgacc accctggcgg cgctgtttgg cgcgctgccg ctggtattgt
    20281 cgggcggtga cggctcggag ctgcggcaac ccctggagat caccattgtc ggcggtctgg
    20341 tgatgagcca gctcctgacg ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc
    20401 tgcggctgcg tttttcgcgt aaacctaaac aaacggtaac cgagtaaatg acagatcttc
    20461 ccgacagcac ccgctggcaa ttatggattg tggctttcgg cttctttatg cagtcgctgg
    20521 acaccaccat cgtaaacacc gcccttccct caatggcgca aagcctcggg gaaagtccgt
    20581 tgcatatgca catggtcatt gtctcttatg tgctgaccgt ggcggtgatg ctgcccgcca
    20641 gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt
    20701 ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac
    20761 gcgcgttaca gggcgttggc ggtgcgatga tggtgccggt cggcaggttg acggtgatga
    20821 aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt tgtcacgtta cccggtcagg
    20881 tcggtccgct gctcggtccg gcgctcggcg gtctgctggt agaatacgcg tcgtggcact
    20941 ggatcttttt gatcaacatt ccggtgggga ttatcggtgc gatcgccaca ttgatgttaa
    21001 tgccgaacta caccatgcag acgcggcgct ttgatctttc cggcttttta ttgctggcgg
    21061 ttggcatggc ggtattaacc ctggcgctgg acggcagtaa aggtacaggt ttatcgccgc
    21121 tggcgattgc tggcctggtc gcagttggcg tggtggcact ggtgctttat ctgctgcacg
    21181 ccagaaataa caaccgcgcc ctgttcagtc tgaaactgtt ccgcactcgt accttttcgc
    21241 tgggcctggc ggggagcttt gccggacgta ttggcagtgg catgttgccc tttatgacac
    21301 cggttttcct gcaaattggc ctcggtttct cgccgtttca tgccggactg atgatgatcc
    21361 cgatggtgct tggcagcatg ggaatgaagc gaattgtggt acaggtggtg aatcgctttg
    21421 gttatcgtcg ggtactggta gcgaccacgc tgggtctgtc gctggtcacc ctgttgttta
    21481 tgactaccgc tctgttgggc tggtactacg ttttgccgtt cgtcctgttt ttacaaggga
    21541 tggtcaactc gacccgtttc tcctccatga acaccctgac gctgaaagat ctcccggaca
    21601 atctggcgag cagcggaaac agcctgctgt cgatgattat gcaattgtcg atgagtatcg
    21661 gcgtcaccat cgccgggctg ttgctggggc tttttggttc gcagcatatc agcgtcgaca
    21721 gcggcaccac acaaaccgtc tttatgtaca cctggcttag catggcgttt atcatcgccc
    21781 ttccggcgtt catctttgcc agagtgccga acgatacgca tcaaaatgta gctatttcgc
    21841 ggcgaaaaag gagcgcgcaa tgaagttctg gcgacccggt attaccggca aactgtttct
    21901 ggcgattttc gccacctgca ttgtcttgct gatcagtatg cactgggcgg tgcgtatcag
    21961 ttttgagcgt ggctttattg attacatcaa acacggcaat gaacagcgat tacagctgtt
    22021 aagtgatgcg cttggcgagc agtatgcgca gcatggtaac tggcgcttcc tgcgcaacaa
    22081 cgatcgcttt gtctttcaga tcctgcgttc atttgaacac gataattcgg aagataaacc
    22141 cggcccgggt atgccgccgc acggctggcg tacccagttc tgggtggtcg atcaaaacaa
    22201 caaagtactg gttggtccgc gagcgccgat tccacctgac ggtacacggc gacccattct
    22261 ggtcaacggt gcggaagtgg gcgcggtgat tgcctcccct gttgagcggc tgacccgcaa
    22321 tactgatatc aatttcgata aacaacagcg gcaaaccagc tggctgatcg tcgccctggc
    22381 aacgttactc gcggcgctcg ccacctttct gctggcgcgc ggtttgctgg caccggtaaa
    22441 acgacttgtc gatggcacgc acaaactggc ggcgggcgat ttcactaccc gcgtaacgcc
    22501 caccagtgaa gatgaactgg gcaaactggc gcaagacttc aaccagctcg ccagcacgct
    22561 ggagaaaaac cagcaaatgc ggcgcgattt tatggccgat atttctcacg aactgcgtac
    22621 gccattagcg gtgctgcgcg gtgaactgga agccattcag gatggcgtgc gcaaattcac
    22681 gccggagacg gtggcgtctt tacaggcgga ggtcggtaca ctgaccaaac tggttgacga
    22741 tcttcatcag ttgtcgatgt ctgatgaagg cgctctcgcc tatcaaaaag caccggtaga
    22801 tttgatccca ctgctggaag tggcgggcgg cgcatttcgc gaacgattcg ccagccgtgg
    22861 cctgaaactg caattttccc tgccagacag tatcaccgta tttggcgatc gcgaccgttt
    22921 aatgcagtta ttcaataact tactggaaaa cagcctgcgc tacactgaca gcggcggcag
    22981 cctgaaaatc tctgccgagc agcacgacaa aacggtgcgc ctgacctttg ccgacagtgc
    23041 gccaggtgtc agtgacgatc agctacaaaa attgtttgaa cgtttttatc gcaccgaagg
    23101 ttcccgcaac cgcgccagcg gcggttccgg gctggggctg gcgatttgcc tgaacattgt
    23161 tgaagcccat aatggccgca ttattgccgc ccattcgcct tttggcgggg taagcattac
    23221 agtagagtta ccgctggaac gggatttaca gagagaagta tgaccgagtt accaatcgac
    23281 gaaaacacac cgcgtatttt gatcgtggaa gatgaaccga agctggggca gttgctcatt
    23341 gattatctgc gtgctgcgag ctatgcgccg acgcttatca gccacggcga tcaggtactg
    23401 gcgtatgtgc gccagacacc accggatctg atcctgttgg atctgatgct ccctggcacc
    23461 gatggcctga cattatgccg ggaaattcgt cgtttttccg atgttccgat cgtgatggtg
    23521 acggcaaaaa tcgaagagat cgaccgcctg ctggggctgg agattggcgc cgatgactac
    23581 atctgcaagc cgtacagccc acgggaagtg gtagcgcgtg tcaaaaccat tttgcgccgt
    23641 tgtaaaccgc aacgcgaact tcagcaacag gatgccgaaa gcccgttgat tatcgacgaa
    23701 ggtcgttttc aggcttcatg gcgcggtaaa atgcttgacc tgacgcctgc ggaatttcgt
    23761 ctgcttaaaa cgctctctca cgaaccagga aaagtgttct cccgcgagca attgctcaat
    23821 catctttatg acgactaccg cgtagtaacc gaccgcacca ttgacagcca cattaaaaat
    23881 ctgcgccgca agctggaatc tctcgacgcc gaacagtcat ttatccgcgc cgtttacggc
    23941 gttggttacc gctgggaagc cgacgcctgc cgcatcgttt agttttagcg acattacttt
    24001 gttagccgga gatggcgttc agacccgatc cggcaacatt attccacgca tggtcagcaa
    24061 actgacatta tctctccgcc ctggcaggca tcagaaaatc gctcatactt taatcagtaa
    24121 acagcacctt tagatgctgt tttcgataag caatttcaat caaggagtca ttatggctgg
    24181 ttggtttgaa ctcagtaaga gcagtgataa tcagttccgg tttgtgctaa aagcgggcaa
    24241 tggtgagact atcctcacca gcgagctgta tacctcaaaa gcctctgcgg aaaagggcat
    24301 cgcgtcggtg cgtagcaaca gcccgcaaga agagcgctat gagaaaaaaa cggcaagcaa
    24361 cggcaaattc tatttcaatc tgaaagccgc taatcatcaa attatcggct ccagccagat
    24421 gtacgccacc gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag
    24481 ccagacggtg aaagacaata cgtaattacg atgccgggcg tgggttgttg gcgtccggcg
    24541 ctttaccaga tcccccctac agcgctacaa tgcccgccct taaagtgggg gcctccccta
    24601 accgcttcat caggtgaagc ggatctgacc tgtcatcaga acgagagaat tatgtttaaa
    24661 ccggaactcc tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc
    24721 gcagatgctg tttatgcggg ccagccgcgt tactccctac gtgtgcgcaa caacgaattc
    24781 aaccacgaaa atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat
    24841 gtcgtggtca acattgcacc gcacaacgcc aagctgaaaa cctttatccg tgacctgaaa
    24901 ccggtggtgg aaatggggcc ggatgcgctg attatgtccg atccagggct gattatgctg
    24961 gtgcgtgagc acttccctga aatgccgatc cacctctcgg tgcaggctaa cgccgtgaac
    25021 tgggcgacgg tgaaattctg gcagcaaatg ggactgaccc gcgtgatcct ctctcgcgag
    25081 ctgtcgctgg aagagattga agagatccat aatcaggtgc cggatatgga gatcgagatc
    25141 ttcgttcacg gcgcactgtg catggcctac tccggtcgct gcctgctctc tggctatatc
    25201 aacaagcgcg acccgaacca gggcacctgc accaacgcct gccgctggga gtacaacgtc
    25261 caggaaggga aagaagatga cgttggcaac atcgtacaca agtacgagcc gattccggtg
    25321 caaaatgttg agccgacgct gggtatcggc gcgccaaccg acaaagtgtt tatgatcgaa
    25381 gaagcccagc gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc
    25441 atgaactcga aagatctgcg cgccatcgcc catgtagaac gcctgaccaa aatgggcgtg
    25501 cattcgctga aaatcgaagg tcgtaccaaa tctttctact attgcgcacg caccgcacag
    25561 gtttaccgca aagctatcga tgacgccgct gcgggaaaac cgttcgatac cagcctgctg
    25621 gaaactctgg aaggtctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact
    25681 cacgacgatt atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt
    25741 ggtgagttta ccggtgagcg caagggggac ctcgcagcgg tagcggtgaa aaataaattc
    25801 tccgttggcg acagtcttga gctgatgacg ccgcaaggca acattaactt tacccttgag
    25861 cacatggaaa acgccaaagg cgaagctatg ccggtcgcac caggcgatgg ttatactgtg
    25921 tggctcccgg tcccgcagga tcttgagcta aattacgcgc tgctaatgcg taatttctcc
    25981 ggggaaacca cgcgtaaccc ccacggtaag tgattaattt cgattatttt tcccggatgg
    26041 aaaattctta gaaaccgatc acatacagct gcatttatta aggttatcat ccgtttcgct
    26101 gaaaaacata atccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat
    26161 ctcccttaca cgggcttatt ttttgtacat aagctgtaaa gaaggattga tccctgataa
    26221 ccgtcttcca ttgaccacat cgatagaatc ccccttcata gcacaatgcc tttcacgtaa
    26281 cggtatcgtg ctcgcccagg ttccggctac gcacagccag aacgcgcatg tttgacgctt
    26341 acaaaaaaat attctcactc tccacatttg aatgtcagac gagcgacgcc atgtaatcct
    26401 gcaccttctg tcttcaggtc aactatctgc atttttttgc cctgagtaac acagaaatgg
    26461 gctgcatcat tttttactat attttctgca ccagatattc tgcccctggc taaagaagct
    26521 tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa tattactttt atatgcaaga
    26581 ccaaatttac cgatacttgt ttcatcatta tgcacagcac aaccagatat aagaaaaata
    26641 ctaattaatg atatagcagc tatctttttc atctcacctt cccccattaa ataccaacga
    26701 cactctctag tgtttaaata taataatggc atgattatta taattgaata ggattataat
    26761 aaatgttctg tacaacattt cctacataag taggaattac ggacattgag gcccttcagg
    26821 gtaactccat gggggcttta atatattata ttgaagatgc cactgtttag ttgaatatta
    26881 ggtatatgct cttttttgaa atttatcggt ggcagccgtt agtattcgct gtccccattg
    26941 caagctcctg gtggtaacca ctgaatcctc catacttgaa ctgacttttt atcctccgac
    27001 tttcatcctg ttctgactcc accttttgtt ttctgctcta cactatctac agaccaatca
    27061 taaaggcaca tacgatcatg gcagaatttc ccgccagctt actgattctt aatggtaaaa
    27121 gtactgacaa tctacccttg cgcgaagcaa ttatgctgct gcgtgaggaa ggaatgacga
    27181 tccatgtgcg ggtcacctgg gagaaaggtg atgccgcacg atttgtagag gaggcccgga
    27241 agttgggcgt cgcaacggtg attgccggtg gtggcgatgg caccattaat gaagtttcta
    27301 cggcgttgat tcagtgtgag ggggatgaca tacccgcgct gggaattttg ccattaggaa
    27361 ccgccaatga ttttgccacc agtgtaggga ttcctgaggc actggataag gcgctgaaac
    27421 tggcaattgc cggtaatgcc attgcgatag atatggcgca ggtcaacaaa caaacctgtt
    27481 ttattaatat ggcgacaggc ggatttggga cgcgtattac cacagaaacg ccggaaaaat
    27541 taaaagccgc gctgggtggc gtctcttaca tcattcatgg cttaatgcgc atggatactc
    27601 tgcaaccgga ccgttgtgaa atccgcggtg aaaactttca ctggcaaggt gacgccctgg
    27661 tcattggtat tggtaacggg cgtcaggccg gtggcggtca acaattgtgc ccgaacgcgt
    27721 taattaacga tggcttgctg caactgcgca tttttaccgg cgatgaaatt cttccggctc
    27781 tcgtatcaac cttaaaatct gacgaagata acccgaatat tatcgaaggc gcttcgtcgt
    27841 ggtttgatat acaagcccca cacgaaatca cttttaatct tgatggcgaa ccgttgagtg
    27901 ggcaaaactt ccatattgaa atacttccgg cggcgttgcg ttgtcgatta ccaccagatt
    27961 gtccattatt gcgttaatca aactattctc tcttgaacca gggagaatcc gtaatgccgg
    28021 atgcggtgta aacaccttat ccggcataca gaacaatact acaccgtaat taattttacc
    28081 cgtgtcttct gcaatgccaa tttatccacc tctgacacag catcatcggt aacaataatg
    28141 tcaatttttt cgattggcag tacctgatta aaaccgcgac gattaaattt cgatgaatcg
    28201 agaacggcaa tcactttatt tgcggcggtg accatcgccc cgctaatcga ataaccttca
    28261 ttaaaggtcg taataccatt aaccgcatca ataccatcag caccgacaaa cattaaatcg
    28321 gcattaatat cctgcaatga acgctcggca atggaaccat gcatcgagcg cgttttatgg
    28381 cggaccgtgc caccacagac gaccagagta atgtctttat tttcggaaag ggcaaacgct
    28441 gccgggagac tgttggtaat caccgtgata tttttagcgg tcattaatcc ttcggcgata
    28501 agcattgtgg tacttccgct gtcgagaata acggtcatcc cttcatggat cattttgact
    28561 gccgcctgcg ctattctgtt ttttggcgcg ctggcgagct gaaatcgctc tttaaacccg
    28621 acttcctggg tctcggtttc actattaccg gacattattt tcgccgcacc gccatgaaag
    28681 cgcgtaacca cgcctttttg ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag
    28741 gcagcaaata ctcccgccag atcctgaaca agcacggttc cctgttcatt cactaattgg
    28801 atgatcttat tccttcgctc gaatgaattc atcgtcattt acccatattc aattgtggct
    28861 agtgtaaacg aagggggacc attggtgaat gatctgtttt gcacaatacc gggccagcat
    28921 cacgctggcc cgcggtttca tggaatgagc aacactttgc cctgcatggc attacgagcg
    28981 atgtcacgca ccgcctgggt gaagctttca aagcttccac ggtgagcgat taatggctcc
    29041 aggcttaact tacgttctgt cagcaaccgg ctcgccgttt cccactcctg ccccggccaa
    29101 gggctggaat agttcatcca actgccgata accgtcagct ctttacgcaa tattttgcca
    29161 aacgttgccg atgttaaatg cagatcctga tgcaacgtgc ccaccagcgc cagttgggca
    29221 tgaggaccgg caatctctac cgccagttcg acagtttgcg gtacgccagc cgtctcgagg
    29281 ataagctgat taaagcgcag ttcgcgtaaa acgctctgca tttgcggcgc gctcatttcg
    29341 ctactgttaa atgtttgcat cgcaccgaaa gattttgcca gtgccagttt ttctgagcta
    29401 atgtcgatcg ccgtcacact ctttgctccc agcgcgacag cgcactgaat agccagcagg
    29461 ccaatggttc cggcaccaat aataataacg tttttattct cacaaccttg cgctaaatga
    29521 aaagcatgca ggccaacggt aatcggctca ataaaagccc catcctcaat aggcatatcc
    29581 gtgggtagag caaagacatt ttttcgctta acgacaatat attcagcaaa tccaccatca
    29641 cgccgcgagc caataaaatc atattttgcg cactgggaat aaaacccttt caaacactct
    29701 ggacaagtaa aacagggtaa taacggcaca caggcaaccg catcgccagg gtgtaaatca
    29761 tcaacaccgg atcccaccgc atcaatatag ccgct
//
LOCUS       DAJTYH010000044        29578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTYH010000044 DAJTYH010000000
VERSION     DAJTYH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..717
                     /locus_tag="OO609_001931"
     CDS             <1..717
                     /locus_tag="OO609_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6633362.1"
                     /translation="ILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGN
                     PRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGF
                     TTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVV
                     DCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGL
                     KKQFDPNGIMNTGTIYPIEK"
     gene            739..2148
                     /locus_tag="OO609_001932"
     CDS             739..2148
                     /locus_tag="OO609_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633363.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2126..2905
                     /locus_tag="OO609_001933"
     CDS             2126..2905
                     /locus_tag="OO609_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6633364.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2902..3762
                     /locus_tag="OO609_001934"
     CDS             2902..3762
                     /locus_tag="OO609_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6633365.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3910..4485)
                     /locus_tag="OO609_001935"
                     /pseudo
     CDS             complement(3910..4485)
                     /locus_tag="OO609_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4502..4762)
                     /locus_tag="OO609_001936"
     CDS             complement(4502..4762)
                     /locus_tag="OO609_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6633366.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4753..6024)
                     /locus_tag="OO609_001937"
     CDS             complement(4753..6024)
                     /locus_tag="OO609_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6633367.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6102..6464)
                     /gene="queD"
                     /locus_tag="OO609_001938"
     CDS             complement(6102..6464)
                     /gene="queD"
                     /locus_tag="OO609_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6633368.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6783..8582
                     /gene="cysJ"
                     /locus_tag="OO609_001939"
     CDS             6783..8582
                     /gene="cysJ"
                     /locus_tag="OO609_001939"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6633369.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8582..10294
                     /gene="cysI"
                     /locus_tag="OO609_001940"
     CDS             8582..10294
                     /gene="cysI"
                     /locus_tag="OO609_001940"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6633370.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10368..11102
                     /gene="cysH"
                     /locus_tag="OO609_001941"
     CDS             10368..11102
                     /gene="cysH"
                     /locus_tag="OO609_001941"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6633371.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11366..11518
                     /locus_tag="OO609_001942"
     CDS             11366..11518
                     /locus_tag="OO609_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6633372.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11712..12803
                     /locus_tag="OO609_001943"
                     /pseudo
     CDS             11712..12803
                     /locus_tag="OO609_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12949..13986)
                     /gene="iap"
                     /locus_tag="OO609_001944"
     CDS             complement(12949..13986)
                     /gene="iap"
                     /locus_tag="OO609_001944"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6633373.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14238..15146
                     /gene="cysD"
                     /locus_tag="OO609_001945"
     CDS             14238..15146
                     /gene="cysD"
                     /locus_tag="OO609_001945"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6633374.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15148..16575
                     /gene="cysN"
                     /locus_tag="OO609_001946"
     CDS             15148..16575
                     /gene="cysN"
                     /locus_tag="OO609_001946"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6633375.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16575..17180
                     /gene="cysC"
                     /locus_tag="OO609_001947"
     CDS             16575..17180
                     /gene="cysC"
                     /locus_tag="OO609_001947"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6633376.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17230..17553
                     /locus_tag="OO609_001948"
     CDS             17230..17553
                     /locus_tag="OO609_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6633377.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17747..18058
                     /gene="ftsB"
                     /locus_tag="OO609_001949"
     CDS             17747..18058
                     /gene="ftsB"
                     /locus_tag="OO609_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6633378.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18077..18787
                     /gene="ispD"
                     /locus_tag="OO609_001950"
     CDS             18077..18787
                     /gene="ispD"
                     /locus_tag="OO609_001950"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6633379.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18787..19266
                     /gene="ispF"
                     /locus_tag="OO609_001951"
     CDS             18787..19266
                     /gene="ispF"
                     /locus_tag="OO609_001951"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6633380.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19263..20312
                     /gene="truD"
                     /locus_tag="OO609_001952"
     CDS             19263..20312
                     /gene="truD"
                     /locus_tag="OO609_001952"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6633381.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20293..21054
                     /gene="surE"
                     /locus_tag="OO609_001953"
     CDS             20293..21054
                     /gene="surE"
                     /locus_tag="OO609_001953"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6633382.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21048..21674
                     /gene="pcm"
                     /locus_tag="OO609_001954"
     CDS             21048..21674
                     /gene="pcm"
                     /locus_tag="OO609_001954"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6633383.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21814..22953
                     /gene="nlpD"
                     /locus_tag="OO609_001955"
     CDS             21814..22953
                     /gene="nlpD"
                     /locus_tag="OO609_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6633384.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23016..24008
                     /gene="rpoS"
                     /locus_tag="OO609_001956"
     CDS             23016..24008
                     /gene="rpoS"
                     /locus_tag="OO609_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6633385.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24128..24535)
                     /locus_tag="OO609_001957"
     CDS             complement(24128..24535)
                     /locus_tag="OO609_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6633386.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24682..25275
                     /locus_tag="OO609_001958"
     CDS             24682..25275
                     /locus_tag="OO609_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6633387.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25275..26222
                     /locus_tag="OO609_001959"
                     /pseudo
     CDS             25275..26222
                     /locus_tag="OO609_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26495..27058)
                     /locus_tag="OO609_001960"
     CDS             complement(26495..27058)
                     /locus_tag="OO609_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633388.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27223..27474
                     /locus_tag="OO609_001961"
     CDS             27223..27474
                     /locus_tag="OO609_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6633389.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27476..29572
                     /locus_tag="OO609_001962"
     CDS             27476..29572
                     /locus_tag="OO609_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6633390.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7214 a         7337 c         8019 g         7008 t
ORIGIN      
        1 atcctgcgtg aaatcatggt ggaagggtat cgtccgtcga tcgctcgttt gtatgacgct
       61 gaagatggca cccaacactt cacccatttt gccgacggaa aatgtgtgct gatctttatg
      121 gctgaaggta accctcgcat tgcgaaggcg acgggcgaag ggattgcgga aatcgttgcc
      181 cgctacccgc agtgccagcg cgtggacagc aaactgattg aaacctggtt taacaacctg
      241 aactggggac cggataaagt ggctgccgaa cgtgtgcaga tcctcaaaac cggcaacatg
      301 ggctttacca ccgaagtgtc cggctgctgg agctgcatcc acgaaatcta cgaaagcgtt
      361 attaaccgta ttcgtactga gttcccgcac gccgacgaca tcaccatgct gggcggtcac
      421 tcctctcata gctatcagaa cggcaccaac atgtacttcg tctatgacta caacgttgtt
      481 gactgtaagc cggaagagga aatcgacaag taccacaatc cgctcaacaa aatcatctgc
      541 gaagaaacca ttcgcctcgg cggttcgatg gtgcaccacc atggcattgg taaacatcgc
      601 gttcactgga gcaagctgga acacggcagc gcgtgggcgt tgctggaagg gctgaaaaag
      661 cagttcgatc ctaacggcat tatgaatacg ggtactatct atccgattga aaaataatgt
      721 atcaggcagc gttccgcgat gacggggcgt tgccttttcg gcttctcagg cgagaagccg
      781 ttcttattac cggacaatga aggggtaaag atgaacactt caccggtgcg aatggatgat
      841 ttaccgctta accgttttca ctgtcgaatt gctacgctca ctttcggcgc acacctgacc
      901 gacggttatg ttctaggcgt cattggttac gccattattc aacttacgcc cgccatgcaa
      961 ctgacgccgt ttatggcggg aatgatcggc ggctcggcgc tccttggttt gtttcttggc
     1021 agcctggttc ttgggtggat ctccgaccat attggtcggc aaaaaatctt caccttcagc
     1081 tttttgctga ttacgctcgc ttcgttcttg caattttttg ccaccacgcc agagcatctt
     1141 attgggctgc gcattttgat cggcattggt ctgggaggcg attactcagt aggtcacacc
     1201 ttgctggctg aattttcccc gcgccgccat cgcggtattt tgctgggcgc attcagcgtg
     1261 gtgtggaccg taggctatgt gttggcaagt attgccggac atcactttat ttccgaaaac
     1321 ccggaggcct ggcgctggtt gctggcatcg gcagctctgc ccgcgttgtt gattacgtta
     1381 ttacgctggg gaacgccaga atcgccacgt tggctactgt gccaggggcg ttttgcagaa
     1441 gctcacgcta tcgtgcatcg ctattttggt ccccatgttt tactgggcga tgaagtggta
     1501 acggcgaccc ataaacacat caaaaccttg ttctcttcgc gttactggcg gcgcacggcg
     1561 tttaacagcg tcttctttgt ctgcctcgta atcccatggt ttgtgattta tacctggctg
     1621 ccaactatcg cccagactat tggtctggaa gatgcgctga ctgccagcct gatgcttaat
     1681 gcgttgttaa ttgtgggcgc gctgctggga ttagttctga cgcacctgct ggcacatcgc
     1741 aaatttttgc tgggaagttt tttgctgctg gcggcaacgc tggtagtaat ggcctgtttg
     1801 ccttccggca gttcattaac gctgctgctt tttgttctct tcagcaccac catttcggca
     1861 gtcagtaatc tggtgggcat tttgcctgcg gaaagttttc ctactgacat tcgctcgctg
     1921 ggagtcggtt ttgccaccgc catgagtcga cttggcgcgg cggtaagtac tggcctgctg
     1981 ccgtgggtgc tggcgcagtg gggaatgcaa gtcaccttat tgctcctagc gacagtgttg
     2041 ttggttggtt ttgttgtgac ctggctatgg gcaccagaaa ctaaagccct cccgctggtg
     2101 gcggcgggaa atgtaggagg tgcgaatgaa cattctgtta gcgtttaaag ccgaaccgga
     2161 tgccggaatg ctggcggaaa aagagtggca ggcggcggct cagggtaata gcggaccgga
     2221 tgtttcgcta ctgcgaagtt tactcggtgc tgatgaacag gccgctgccg cgctgctgtt
     2281 agcgcagaga aaaaacggta cgccgatgtc tttaaccgcg ttgagcatgg gggatgaacg
     2341 ggcgttgcac tggctgcgct atctcatggc tcttgggttt gaggaagctg tattgctgga
     2401 gacggcggca gatctgcgct ttgcgccgga atttgtcgct cgccatattg ccgaatggca
     2461 gcgtcagaat ccgctggatc tgataattac cggcagccaa agcagcgaag ggcagaatgg
     2521 gcaaacgcca tttttgctgg cggaaatgct gggctggccc tgcttcaccc aggtggagcg
     2581 tttcactctc gacgcgctgt ttatcaccct cgaacagcgt actgaacatg ggttgcgctg
     2641 ttgccgggtt cgcttgcctg cggtgattgc cgtgcgtcag tgcggtgaag tggccttgcc
     2701 agtaccggga atgcgccagc gaatggctgc ggggaaagca gaaattatcc gtaaaactgt
     2761 tgctgccgaa atgccagcga tgcagtgtct gcaactggca agagcagagc aacgacgcgg
     2821 agcgacgctg attgatgggc aaacggtagc agaaaaagct caaaagctat ggcaggacta
     2881 tttacgccaa aggatgcagc catgaatatc gcaattgtga caataaatca ggagaatgcc
     2941 gcaatagcga gctggctggc ggcgcaggat ttcagcggtt gtacgctggc gcactggcaa
     3001 atagaacctc agcccgtagt ggcggaacag gtgctggatg cgatggtgga acagtggcag
     3061 cgaacgcctg cggacgttgt tctctttccg ccgggaacgt ttggcgatga actgtcgacc
     3121 cggctggcct ggcgtttaca tggtgcaagc atctgtcagg tgacttcgtt agatattcca
     3181 accgtaagca tgcgcaaatc ccactgggga aatgcgttaa cggcgacgct gcaaacagaa
     3241 aaacgcccgt tatgcctttc cctggcgcgt caggcgggag cagcgaaaaa cgccacgttg
     3301 ccctctggaa tgcagcaact gaacattgtc ccaggtgcgc ttcccgactg gctggttagc
     3361 acagaggatc tgaaaaatgt tagccgcgac ccattagctg aggccagacg ggtgctggtg
     3421 gtgggacagg gcggagaggc agataaccag gaaatcgtta tgctggcaga aaagctggga
     3481 gccgaagtgg gctacagtcg ggcgcgagtg atgaacggtg gcgttgatgc tgaaaaagtg
     3541 attggtattt ccggacatct gctggcacct gaggtgtgca ttgttgttgg cgcttccggc
     3601 gcagcagcgt taatggcggg cgtacgcaat agcaagttta tggtggcgat taatcatgac
     3661 gccagcgcag cggtattttc acaggcagat attggcgtgg ttgatgactg gaaagtggtg
     3721 ctggaggcac tggtgacaaa cattcacgca gaataccaat aacgtgctgc cggatgatcc
     3781 tgcctacggt tctggactgg ggacctgtag gcctgataag acgcgttaag cgtcgcatca
     3841 ggcgctgaat gtcggatgtg gcgtgcggcc aacaaatcaa aacaaattca attggttacg
     3901 attatcccgt caaagcaatt ttttcgctaa cgtccagact ccggtattcg tggtggaaag
     3961 cgccacgaca cccgcgttaa tcgcattacg cgcatcttct tcatcgcata ccagcccgcc
     4021 agcaatcagc ggttggcgga ttttttccgt cacccagccc agcactttgg gcatgcagcc
     4081 tggcaggatc tcaatacaat ccggattcga ttgcgcaact tacttatcaa tgttgtgaaa
     4141 cgaaatcgaa tcaacaataa acaggtgatg aatacagaaa aaaacttccg ctcttgccgc
     4201 ttttagcatc gatgctttgg tgctgataat tccatccgcc tcggtcacca gtttcaaaaa
     4261 ctgaatcacc acttctttat ttgacgcgcc ttccagcaaa tcaacatgaa taaaagcata
     4321 tttcccggcg ttcttaatct ttttaacgat attactgatg gtgcagatat tgccgtacaa
     4381 cacggaaata aactggcatt cagaatcgat ggccagttgc aggctggcat tatctttaac
     4441 ggcagcaatc accggattct ggcggagcag gtgtaagagg ggcataaatt atccttatta
     4501 tttatccaaa gcggtaggtg atgccgaatc cggaaggcgg atagcgccac tgttgtagtg
     4561 tggattcgtc gcacagcaaa cggcaggtgc cacactccag gcagccgcga tagtcaatac
     4621 gtaagtcacc ttcctgtgtg agagaaaaaa gacctaccgg acaggcgtta atcaaccgtt
     4681 gtaccatctg acgctcaacg gagtcagccg gaacaatgtg cggcgcatca gcaacgcgcc
     4741 agagattacg ggctacagac atcgcagact cctgataaca tcgcccgcca gatgccacag
     4801 gccatgacga cgtaaatgat gccagaataa ctggcgcagc ggtggaacag gtttatcacc
     4861 ctgatcccat aaatcgcggg aaatatcctg cattaacgca ggccacgccc gatacctgcc
     4921 agggcgttgc aaaagcgccg gaacatgctg ataacgttgt agaacatccc atagcaggct
     4981 gcgctctacg ttgtgatgat aaagcgcaaa cagattttgc ggctcgcggt gctggcaggc
     5041 gcttattagc gtttgtgccg ccgcctgtgc gccagtcagc gccatatcca tgccgcgcac
     5101 ggaaattccg gtattgacgc aactgcgcaa cgcatcgccc accagcagcc agccgttacc
     5161 ggcgtattgc accggcatac tgtgcaagcc accttctggc accagatgcg caccatactc
     5221 cagtgattcc gtgtttttga taagcgtgcg cactgccgga tgcgctttaa agcgagtcag
     5281 cagctcgctt gccggaacac gactttgcgt aagggaagag agcgggcaaa caatccctaa
     5341 cgagagcgtt tgttgattag tataaagaaa tgcgccgccg ggtaagtcat cacagattcc
     5401 gccgctgaac agcaacgctg cgccttcgtt attctccaga tgaaaacgtt cttcaatagc
     5461 ggatgtttcc agcgacagca cttctttgat ccccaacgcc atcgcttcgc cagcaggacg
     5521 agtcactaac ccgtgacgtt cagccaggac gctgttggca ccttctgcca gcaccacata
     5581 acgggcgcgg agaatatcgt caccacaaat aacgccacag actctgccgt tttcttcata
     5641 cagtgcatcc accgtcgctc cggggatgca ttcgacacct tctttttcgg cttcggcaac
     5701 cagccacgga tcgaaccgtg cacgtaatac attccaggat tcaccgccgg gctgtaagct
     5761 ggaaaacgtc gttgcgccat ccggcgttaa cagggaaagg ctttcgtgag tgatgcgtcg
     5821 ttcaagaggc gcggtcagat gaaattgcgg gaggagttcc gcgagtgcat gggtatataa
     5881 ccgcctgccg gaaagatttt tgctgccggg gatttcagcg cgttcgagta acaaaacgga
     5941 taaatccgct cgcgcgcagc gtaacgcgca agcggtgcct gcaataccgg caccaataat
     6001 aataatgtcg cagtcgtctt ccatgtcatg ccccagatat ccagaacaca acaccctaac
     6061 atagcgttac taaagggaaa ttgaccgccg acacactctt atcattcgcc gcgatagata
     6121 caccctgcgg tgcaggtttc ttttaccatc accgcactta acagcggcac aacgggttta
     6181 acctgatccc aaatccattt tgctaaaact tcgctggttg gattttccag acctggaata
     6241 tcattgagat aatggtgatc gaggcgctca taagttggct taaacgccgc tttcagttca
     6301 gcgaaatcga taatccagcc cgtatgcgga tcgacttccc cggtaatttc cagtcgcacc
     6361 ataaaggaat gcccgtgcag gcgaccacat ttatgccctt ccgggacatg tggtaagcgg
     6421 tgagcggctt cgaaggtgaa atctttaaat aacgtggtgg acatcataat ttctctacag
     6481 gacgataaaa ccgccgtaga gtaccggaaa ggctgttttt tcgcattatc taaaacgact
     6541 ttgttgcgca aaatcgctga tttatcttaa tgattggcta aattcatttg tttttcatta
     6601 ggttggttaa tctattttgt tgttaaagac tattgctaaa acaggttagt cgatttggtt
     6661 attagttatc gctatcccgt ctttaatcca caccgttcac cccgttaacc ttaccttctc
     6721 ttctgtttta tgggcgctga cagggcgcag aaacagcttt gcttactgga acataacgac
     6781 gcatgacgac acaggtccca ccttccgcgt tgcttccgtt gaacccggag caactggcac
     6841 gccttcaggc ggccacgacc gatttaacgc ctacccagct tgcctgggtt tctggctatt
     6901 tctggggcgt gctcaaccag cagcctgttg cgcttgcagt gacgccagcg cccgccgcag
     6961 aaatgccggg tataacgatt atctctgctt cgcaaaccgg caatgcgcgc cgggttgctg
     7021 aagcattacg cgatgattta ttagcagcaa aactgaacgt taagctggtg aacgcgggcg
     7081 actataaatt caaacaaatc gccagcgaaa aactgctcat cgtagtgacg tcaacgcaag
     7141 gggaagggga accgccggaa gaagccgtcg cgctgcataa gttcctgttc tccaaaaaag
     7201 cgccaaagct ggaaaacacc gcgtttgccg tgtttagcct cggcgatagc tcttatgaat
     7261 ttttctgcca gtccgggaaa gattttgaca gcaagctggc ggaactgggg ggtgaacgcc
     7321 tgctcgaccg tgtcgattct gatgttgaat accaggctgc tgccagcgag tggcgcgccc
     7381 gcgtggttga tgcgcttaaa tcgcgtgcgc ctgtcgcggc accttcgcaa tccgtcgcta
     7441 ctggcgcggt aaatgaaatc cacaccagcc cgtacagcaa agacgcgccg ctggtggcta
     7501 gcctctctgt taaccagaaa attactgggc gtaactctga aaaagacgtt cgccatatcg
     7561 aaattgactt aggcgactcg ggcctgcgtt accagccggg tgacgcgctg ggcgtctggt
     7621 atcagaacga tccggcactg gtgaaagaac ttgtcgaact gctgtggctg acaggcgatg
     7681 aacctgtcac tgtcgagggc aaaacgttgc ctctgaacga agcgctacag tggcacttcg
     7741 aactgaccgt caacaccgcc aacattgttg agaattacgc cacgcttacc cgcagtgaaa
     7801 cactgctgcc gctggtgggc gataaagcga agttacagca ttacgccgcg acgacgctga
     7861 ttgttgacat ggtgcgtttc tccccggcac agcttgatgc cgaagcgcta attaatctgc
     7921 tgcgcccgct gaccccgcgt ctctattcca ttgcctcctc gcaggcggaa gtcgagaacg
     7981 aagtacacgt caccgttggt gtggtgcgtt acgacgtgga aggccgcgcc cgtgccggtg
     8041 gtgcctccag cttcctcgct gaccgcgtgg aagaagaggg cgaagtccgc gtatttatcg
     8101 aacataacga taacttccgc ctgcccgcta acctggaaac cccggtgatt atgattggcc
     8161 caggcaccgg tattgcgccg ttccgcgcct ttatgcagca acgcgccgct gacgaagcgc
     8221 caggtaaaaa ctggctgttc tttggcaatc cgcattttac ggaagacttc ctgtatcagg
     8281 tggagtggca gcgctacgtc aaagagggtg tgctgacgcg gatcgatctc gcctggtcgc
     8341 gcgaccaaaa agaaaaagtt tacgtacaag ataaactgcg cgaacagggc gcggagctgt
     8401 ggcgctggat caatgacggt gcccacattt atgtctgcgg cgacgctaat cgcatggcga
     8461 aagacgttga gcaggcactt ctggaagtga ttgccgaatt tggtggcatg gacaccgaag
     8521 cggcggatga atttttaagt gagctgcgcg tagagcgccg ttatcagcga gatgtctact
     8581 aatgagcgaa aaacatccag ggcctttagt ggtcgaagga aaactgacag acgccgagcg
     8641 catgaagctt gaaagcaact acctgcgcgg caccattgcg gaagatttaa acgacggtct
     8701 gaccggcggc tttaagggcg ataacttcct gctgatccgc ttccacggca tgtatcagca
     8761 ggatgaccgc gacatccgcg ccgaacgtgc tgaacagaag ctggagccgc gccacgcgat
     8821 gctgttgcgc tgccgtctgc cgggtggagt tatcaccacc aaacagtggc aggcgatcga
     8881 taaatttgcc ggtgaaaaca ccatctatgg cagcattcgc ctgaccaacc gccagacgtt
     8941 tcagttccac ggcattctga aaaagaacgt caaaccggtg caccagatgc tgcactcggt
     9001 cggtcttgat gcgctggcga ctgctaacga catgaaccgt aacgtactct gcacctcgaa
     9061 cccttacgag tcgcagctgc acgcagaagc gtacgagtgg gcgaagaaga tttctgaaca
     9121 tctgctgccg cgtacccgcg cgtatgcgga gatctggctc gatcaggaaa aagtcgccac
     9181 caccgatgaa gaaccgatcc ttggtcagac ttacctgccg cgtaagttca aaaccacggt
     9241 agtgatcccg ccgcagaacg atattgatct gcacgccaac gacatgaact tcgtggcgat
     9301 cgcggaaaac ggcaagctgg tgggcttcaa cctgctggtg ggcggtgggc tttctatcga
     9361 acacggtaac aagaaaacct acgcccgcac ggcgagtgag tttggctatc tgccgctaga
     9421 gcatacgcta gcggtggcgg aagccgtcgt gacaactcag cgtgactggg gtaaccgaac
     9481 cgatcgtaaa aatgccaaaa ccaaatacac actggagcgc gtgggggttg agacgtttaa
     9541 agcggaagtg gagcgtcgcg cggggatcaa atttgaaccg atccgcccgt atgaatttac
     9601 aggacgcggc gatcgtattg gctgggttaa gggcattgat gataactggc acctgacgct
     9661 gtttatcgaa aatggtcgca tccttgatta tccggggcgt ccgctgaaaa ctggcctgct
     9721 ggagatcgcg aagatccaca aaggtgattt ccgcattacg gcgaaccaga atctgatcat
     9781 cgccggtgtg ccggaaagcg agaaagcgaa gatcgagaaa attgccaaag agagcgggtt
     9841 aatgaatgcc gtcacgccgc agcgtgaaaa ctcgatggcc tgcgtgtcat tcccgacttg
     9901 cccgctggcg atggcggaag cggagcgttt cctgccgtct tttatcgaca acatcgataa
     9961 tttaatggtg aaacatggtg tcagcgatga gcatatcgtg atgcgtgtaa caggctgccc
    10021 gaacggttgt ggtcgcgcga tgctggcgga agtgggcctg gtgggtaaag caccgggtcg
    10081 ctacaacctg catcttggcg gcaaccgcat tgggacacgt atcccacgga tgtataaaga
    10141 aaacatcacc gagccggaaa tcctggcgtc gcttgatgaa ctgatagggc gctgggcgaa
    10201 agagcgcgaa gcgggtgaag gcttcggcga ctttacggtg cgtgcgggca tcattcgccc
    10261 ggtgctcgat ccggcgcgcg atttgtggga ttaaccatca gcccggtctt gtaggcctga
    10321 taagacgcgt gagcgtcgca tcaggcaagg caaacagtga ggaatctatg tccaaactcg
    10381 atctaaacgc cctgaacgaa ctgccgaagg tagatcgcat tctggcgctg gcagaaacta
    10441 acgccgaact ggaaaaactg gacgctgaag gccgcgttgc ctgggcgctg gataatctgc
    10501 ccggtgaata tgtactttct tccagctttg gcattcaggc ggcggtgagc ctgcatctgg
    10561 tgaatcagat tcacccggat attccggtga tcctcaccga caccggctat ctgttcccgg
    10621 aaacctaccg ctttattgac gagttaacgg acaaactcaa gctcaacctg aaagtgtacc
    10681 gcgctaccga aagcgccgcc tggcaggaag cacgctacgg caaactgtgg gaacagggcg
    10741 ttgaaggcat tgaaaagtac aatgacatca acaaagtcga accgatgaac cgggcgctga
    10801 aagaactgaa tgcgcaaacg tggtttgctg gcctgcggcg tgaacaatcc ggcagccgtg
    10861 ccaatttacc ggtgctggca attcagcgtg gcgtatttaa agtgctgccg attatcgact
    10921 gggataaccg aactatttat cagtacctgc aaaaacatgg cctgaaatat cacccattat
    10981 gggacgaagg atatttatcg gtgggcgata cccatacaac ccgtaaatgg gaacccggca
    11041 tggcggaaga agaaacgcgt ttctttggct taaaaaggga atgtggatta cacgaagggt
    11101 aaatattgcc ggataaatta aacgcctctg tcagaaatga tgggggcgtt tttattttgc
    11161 gagcagcatt ccggcattta aaggtgaaca tcgcagggaa aattaacagg tatagaatgg
    11221 cgttgggtgc ttgaggctgt ctggcgttaa gcattcgcga ggttccagat ggacaaaagc
    11281 cccaggcgat atttctatca acctgaggct cgcgttcgaa cgtagcaatt cggatgttag
    11341 tctcttcttt gcagggaggc aagacatgct gacaaaatat gcccttgtgg caatcatcgt
    11401 actgtgttgt acagtactgg gattcacgtt gatggtaggt gactcgttat gtgagttgag
    11461 tatcagagaa cgtggtatgg agtttaaggc agttctcgct tacgaatcga agaagtagcc
    11521 accacgcggg ggtaaaaccc cgcgtactga attgtgatgg tttggtctcc tgcacccttt
    11581 ttttaaattt atatttttta tgtgaatata tagtctctat ttattattat gaatttcaaa
    11641 tgaaaatcat taatttattc ataatgtaaa tgttttattg gctgatatat atcatagata
    11701 gtttttaaaa tatgcgtaaa tatcctttaa gtttactgaa ggataaaaat attgtgactt
    11761 tctttgattt ctggggaaaa aaccgacgtg gcgagaaaga gggtatcgac gactatcacc
    11821 ttctttgctg gcatttgctg gatgtggccg caatgggcta tttaatggtt aaaagaaatt
    11881 gcttcgggct ggctgattat tttcgtcaat tagggatttc tgacaaggaa caggcagctc
    11941 gatttttcgc ctggttgctg tgctggcacg atattggaaa atttgcccgc tcttttcaac
    12001 aactttacct gccccctgaa ttcaagattc aggaaggtgc cagaaaaaat tacgaaaaaa
    12061 tctctcattc aacgctgggc tactggttgt ggaatcatta tttaagtgaa tgtcaggagt
    12121 tacttccttc atcttcgctc tctccgcgca aacttaggcg agtgatagaa atgtggatgc
    12181 ccgtgacgac agggcatcat ggtcggcccc ctgatcgtat ggatgaattg gataattttc
    12241 tgcctgaaga caaagctgca gcacgagatt ttctccttga aatcaagcca ctgtttccgc
    12301 gaatagagat ccccacattc tgggatgatg acgagggcgt tgaacttata aaacaacttt
    12361 cctggtatat ctctgcaacc gtcgtacttg cagactggac agggtcgtca acgcgatttt
    12421 ttccacgcgt cgcacaggca atggatattg aagattactg gcaaaaggct ttagttcagg
    12481 ctcaaaacgc attaaccgtc tttccgccac aagcagaaac cgcgcctttc accggaatta
    12541 atacgctgtt tccttttatt gagaatacga caccgttaca gcaaaaggtg ctggatctgg
    12601 atatcaatca gccaggacca cagttgttta ttctggaaga tgtgaccggc gcaggtaaaa
    12661 cagaagcggc gcttatcctg gcgcacaggt tgatggctgc gggtaacgct caggggctgt
    12721 tttttggcct gccaacaatg gcaacggcca atgccatgta cgatcgactg gtcaaaacct
    12781 ggcttgcttt ctattcgcca gaggtgttcc ccgctccagc ggagataaac cggcgcaata
    12841 cggtgcggtt ttcccggaag taaaccggac gcactggatg cgatgatggg tatcacttag
    12901 aattccccgc ccctgcggta gaactcccag ctcccatttt caaacccatc aagacgcctt
    12961 cgccagctcc ttcaccaacg gtagcattat ccgcataacg tcacggcagc gacgttctat
    13021 tcttccagga agtgccttat caatatgttg ttgattatcc agtcttacgt catgccagct
    13081 atttcccgcc gggaaggcag gtgtttttgc gcgttgctga taaccatcct tatttccaag
    13141 attccagtta gtcgcttcca ccgaaagtac agcaatgccc gctttgtcga atatttctgc
    13201 gtcattacaa cccccagtgc ctttcggata atttttattc aaacccggat tggtcgttgc
    13261 ggctattccg tgactgcgtg caattgccag cgccctgtcg cgcgttaatt tccttactgc
    13321 ttcaggggtt tttacaccgc tgttgaaata caatttatcg ccaacaatta agttatcgag
    13381 attaatcacc agcagcgtat ttttcttttc ggtgtcactc atccgcttga gtaaattctc
    13441 agcgcctaat tttccttcct cttcgccgct ggtcgccaca aatcgaatac catactcggt
    13501 aggcgtattt ttcaggcgtt ctgccagttc cagcatgaca cctaaacctg cggcgttatc
    13561 atctattcct tgtaacgtca gtccgccgag attggcatct gcgtcagcat cgctcagcgg
    13621 ggcgtaagta tccagatgcg ccataatgat gatctgctgc ggcgctttgc cttcatgagc
    13681 ggcaatcacc gtacttcctg tcacgttatg ccagttcttg cgattatcgc gggcggtata
    13741 aatataccga ctattaaatg tccgaatatc actgcgataa cccatttgct gaaactgttg
    13801 gcgaatataa tcggcagata acatttctgc tggagttccg gtcatgcgtc ccggaaagaa
    13861 agtagcaata tgtcgtgcct gggtattagc aaaatcgcca ggtttaggtg acgaggcgtg
    13921 tacggggaga ataaagcata cgccgagcgc cagggcagcg gtacggtggc gcaatgcgga
    13981 aaacatagtg agtccttaaa taccatgcaa atttttttac cgccatagta tgaaactgcc
    14041 gctgcgctaa aacaatttca aatcttccta aacgcccgaa atccggtgcc ttaagcactt
    14101 tttgatatta gctttgccaa atcgttattc cgttaaggaa ctactcattc taattggtaa
    14161 tttcattcgt tctcttacgc tccctatagt cgaaacatct gatggcaaga aaatagcggt
    14221 attgcaaagg aacggttatg gctcaaaaac gacttactca cctgcggcaa ctggaggcgg
    14281 aaagcatcca cattattcgc gaggtggcgg cagaattctc aaatccggtg atgctctact
    14341 ctatcggtaa agattccagc gtcatgctgc atctggcgcg caaggcgttt tatccaggta
    14401 cgctgccttt cccgttgctg catgtcgata ccggctggaa attccgcgag atgtatgagt
    14461 tccgcgatcg tacggcgaaa gcctacggct gcgagctgct ggtgcataaa aacccggaag
    14521 gcgtggcgat ggggattaat ccattcgtgc acggcagcgc gaaacatacc gatattatga
    14581 aaactgaagg cctgaaacag gcgctgaaca aatacggttt tgatgccgcc ttcggtggtg
    14641 cgcgccgtga cgaagagaaa tcccgcgcta aagagcgaat ctactctttc cgtgaccgct
    14701 tccatcgctg ggatccgaaa aatcagcgcc cggagctgtg gcacaactac aacgggcaaa
    14761 ttaacaaagg cgaaagcatc cgcgtcttcc cgctctctaa ctggaccgag caggatatct
    14821 ggcaatacat ctggctggaa aatatcgaca ttgtgccgct gtatctcgct gcggaacgtc
    14881 cggttctgga acgcgacggt atgttgatga tgattgatga caaccgtatc gacctgcaac
    14941 cgggcgaagt gatcaaaaaa cggatggtgc gtttccgtac gctgggctgc tggccgctga
    15001 ccggtgcggt ggagtcaaat gcacaaacac tgccggaaat catcgaagag atgctggttt
    15061 ccaccaccag tgaacgtcag ggtcgcgtga ttgaccgcga ccaggcggga tccatggagc
    15121 tgaaaaaacg tcaggggtat ttttaagatg aacaccgtac ttgcacaaca aatcgccaat
    15181 gaaggcggcg tcgaagcctg gatgattgcg caacaacata aaagcctgct gcgttttctg
    15241 acctgtggta gcgtcgatga cggcaaaagt accctgattg gtcgtctgct gcacgatacc
    15301 cgccaaatct acgaagatca gctctcatcg ctgcataacg acagtaagcg tcacggaacc
    15361 cagggcgaaa agctggatct ggctctgctg gtggacggcc tgcaagctga gcgcgaacag
    15421 ggcatcacta ttgatgtggc ctaccgctat ttctctaccg agaagcgtaa atttattatc
    15481 gccgacaccc cagggcacga gcagtacact cgcaatatgg cgactggcgc atcgacatgt
    15541 gaactggcga tcttactgat tgatgcccgt aaaggcgtgc tcgatcaaac ccgtcgtcac
    15601 agttttatct ccacactgtt ggggatcaaa catctggtcg tggcgatcaa caaaatggat
    15661 ctggtggatt acagtgaaga gacgttcacc cgtattcgtg aagattatct gacctttgcc
    15721 gggcagctgc cgggtaatct ggatatccgc tttgtgccgc tctccgcact ggaaggtgac
    15781 aacgtggctt cgcaaagtga aagtatgccg tggtacagcg gtctgacact gctcgaagtg
    15841 ctggaaaccg tggagatcca gcgagtggtg gatgcccagc caatgcgctt cccggtgcag
    15901 tacgttaacc gcccaaatct cgattttcgt ggctacgccg gaatgctggc atccggtcgc
    15961 gtggaagtcg ggcaacgtgt aaaagtgctg ccctctggtg tggaatcaaa cgtcgcgcgg
    16021 atcgtgactt ttgatggtga tcgcgaagaa gtctttgccg gagaagcgat caccctggtg
    16081 ctgacggatg agatcgatat cagccgtggt gatctgctgc tggcggcaga cgaagcgtta
    16141 ccggcggtgc agagtgcgtc ggtggatgtg gtatggatgg cggaacagcc gctttccccg
    16201 ggccagagtt atgacatcaa aattgccggt aagaagacgc gtgctcgtgt tgatggcatt
    16261 cgttatcagg ttgatattaa taaccttacc caacgcgaag ttgaaaacct gccgctgaac
    16321 ggcatcggcc tggtggatct cacttttgac gagccactgg tgttagatcg ctatcagcaa
    16381 aatccggtta ccggtgggct gatttttatc gatcgcctga gcaatgtgac cgtgggtgcc
    16441 ggtatggtgc acgagccagt tagccaggca acagctgcgt catctgaatt cagtgcattc
    16501 gaactggaat tgaatgccct ggttcgtcgc cactttccgc actggggcgc gcgcgatttg
    16561 ctgggggata aataatggcg ctgcatgacg aaaacgtcgt ctggcatagt catccggtca
    16621 ctgtgcaaca acgcgaacta caccacggtc atcgtggtgt agtgctgtgg tttaccggcc
    16681 tctccgggtc cggtaaatca acggtcgccg gggcgctgga ggaggcgtta cataaactca
    16741 gcgtcagtac gtatctgctg gatggcgaca atgttcgcca cggattatgc agcgatctcg
    16801 gttttagcga tgccgatcgt aaagagaata tccgtcgcgt cggtgaagtg gcgaatttga
    16861 tggttgaagc cggactggtg gtgctgaccg catttatctc gccacaccgc gccgaacgcc
    16921 agatggttcg cgaacgcgta ggagaagggc gctttattga agtgtttgtc gatacgccgc
    16981 tggcgatttg cgaagcccgc gatccaaaag gcttatataa gaaagcgcgt gccggtgaac
    17041 tgcgcaactt tacgggaata gattccgttt acgaagcgcc tgaatcggca gaaattcatc
    17101 tcaatggtga acaattagta acaaatttgg tacagcaatt attagatctg ttgagacaga
    17161 acgatattat cagatcctga gacaccaccg ggtttccatg cccggttcgt caaagtaaca
    17221 ggatcaaata tgcgtaatag ccataacatt acactaacaa ataacgacag ccttactgag
    17281 gatgaagaaa ctacatggtc actgcctggt gccgtggtcg gttttatctc ctggttattt
    17341 gcgctggcga tgccgatgtt gatttatggc tctaacacgc tgttcttctt tctctacacc
    17401 tggcctttct ttctggcact gatgcctgtc gcggtagtgg tggggattgc gctgcattca
    17461 ttgatggacg gaaagctacg ctacagtatt gttttcaccc tggtgacagt aggcattatg
    17521 tttggcgcac tgtttatgtg gctactgggc taatttgtac tttcttgtct cctctgttca
    17581 taattcaaac cgtaactaat aatgagatta tgttctgcac gccctgggta tacgtaacaa
    17641 tgggcaaatg tggtacattt gcccgcgttg tcgcggtatc cccaacagag gatgtagagt
    17701 cgtcttcgga tgcatgggat gatgatgcag tttttcaggg ggcaggatgg gtaaactaac
    17761 gctgctgttg ctggctattc tggtctggct acagtattcg ctgtggttcg gtaagaacgg
    17821 tatacatgac tatacccgcg tcaatgatga tgtggcggca ctgcaagcta caaacgcgaa
    17881 acttaaagcg cgaaacgatc aactttttgc cgaaattgac gatctcaatg gcggccagga
    17941 ggcgctcgaa gagcgtgcgc gtaatgaact cagcatgacc aggccgggcg aaacttttta
    18001 tcgtctggtg cctgacgcgt cgaagcgcgc acagtctgcg gggcaaaaca atcgataaat
    18061 cagcccggga attaacatgg caaccactca tttggatgtt tgcgccgtgg ttccggcggc
    18121 cggatttggc cgtcgaatgc aaacggaatg tcctaagcaa tatctctcaa tcggtaatca
    18181 aaccattctt gaacactcgg tgcatgcgct gctggcgcat ccccgggtga cacgtgtcgt
    18241 cattgccata agtcctggcg atagccgttt tgcacaactt cctctggcga atcatccgca
    18301 aatcaccgtt gtagatggcg gtgatgagcg tgccgattcc gtgctggcag gtctgaaagc
    18361 cgctggcgat gtgcagtggg tattggtgca tgacgccgct cgtccttgtt tgcatcagga
    18421 tgaccttgcg cgattgttgg cgttgagcga aaccagccgc acgggaggga tcctcgccgc
    18481 accagtgcgc gatactatga aacgtgccga accgggcaaa aatgccattg ctcataccgt
    18541 tgatcgcaac ggcttatggc acgcgctgac gccgcaattt ttccctcgtg agctgttaca
    18601 tgactgtctg acgcgcgctc taaatgaagg cgctaccatt accgacgaag cctcggcgct
    18661 ggaatattgc ggatttcatc cgcagttggt cgaaggccgc gcggataaca ttaaagtcac
    18721 gcgcccggaa gatttggcac tggccgagtt ttacctcacc cgaaccatcc atcaggagaa
    18781 tacataatgc gaattggaca cggttttgac gtacacgcct ttggcggtga aggcccaatt
    18841 atcattggtg gcgtacgcat tccttacgaa aaaggattgc tggcgcattc tgatggcgac
    18901 gtggcgcttc atgcgttgac cgatgcattg cttggcgcgg cggcgctggg ggatatcggc
    18961 aaactgttcc cggataccga tccggcattt aaaggtgccg acagccgcga gctgctacgc
    19021 gaagcctggc gtcgtattca ggcgaagggt tatacccttg gcaacgtcga tgtcactatc
    19081 atcgctcagg caccgaagat gttgccgcac attccacaaa tgcgcgtgtt tattgccgaa
    19141 gatctcggct gccatatgga tgatgttaac gtgaaagcca ctactacgga aaaacttgga
    19201 tttaccggac gtggggaagg gattgcctgt gaagcggtgg cgctactcat taaggcaaca
    19261 aaatgattga gtttgataat ctcacttacc tccacggtaa accgcaaggc accgggctgc
    19321 tgaaagccaa tccggaagac tttgtggtgg tggaagattt gggctttgag cctgatggtg
    19381 aaggtgagca tattctggtt agaatcctca aaaacggctg caatacccgt tttgtggcgg
    19441 atgcactggc gaaattcctg aaaattcatt cccgtgaagt cagcttcgct gggcaaaaag
    19501 acaaacatgc tgtaacggaa cagtggttat gtgctcgcgt gccgggcaag gaaatgcccg
    19561 atctgagcgc ctttcaactg gaaggctgcc aggtgctgga gtatgcgcgg cacaagcgca
    19621 agctgcgttt aggggcgctg aaaggtaacg cctttaccct ggttctgcgc gaagtgagca
    19681 atcgcgatga cgttgaacaa cgtctgatcg atatttgcgt aaaaggtgta ccgaactact
    19741 tcggtgccca acgttttggg attggcggta gcaacttgca gggcgcgctg cgctgggcgc
    19801 aaaccaatac tccggtgcgc gatcgcaata aacggagttt ttggttgtcg gcagcccgca
    19861 gtgcgttgtt taatcagatt gttgctgagc gcctcaaaaa agcagacgtt aatcaagttg
    19921 ttgacggcga tgcgctacaa ttagccggac gtggtagctg gtttgtagca accaccgaag
    19981 aactggcgga attacagcgt cgcgtcaacg ataaagagct gatgataacc gccgcattgc
    20041 cgggcagtgg cgaatgggga actcagcgtg aagcgctggc attcgaacaa gcagctgtcg
    20101 ccgcagaaac tgaattacaa gctttactgg tgcgcgaaaa agttgaagcc gcgcgcagag
    20161 cgatgctgct gtatccgcaa caattaagct ggaactggtg ggatgacgtc accgtagaga
    20221 tccgtttctg gcttccggcg ggtagttttg caaccagcgt tgtcagggaa cttatcaaca
    20281 caacaggtga ttatgcgcat attgctgagt aattatgacg gggtacatgc acccggtata
    20341 caaacgctgg cgaaagcctt gcgtgagttt gctgacgttc aggtggtcgc ccccgatcgt
    20401 aaccgcagcg gcgcttcaaa ttctctgaca ctggaatcct ccctgcgcac gtttaccttt
    20461 gaaaatggtg atattgctgt gcaaatggga accccgaccg attgtgtcta tcttggcgtg
    20521 aatgctctga tgcgtccgcg cccggacatt gttgtgtccg gaattaacgc cgggccgaat
    20581 ctgggggatg atgttattta ttccggtacg gtagccgccg cgatggaagg ccgtcattta
    20641 ggttttccgg cgctggccgt ctcgcttgac gggcataaac attacgacac tgccgcggcg
    20701 gtaacctgtt caattttgcg cgcactgtgt aaagagccgc tgcgcaccgg acgtattctt
    20761 aatattaacg ttcaggattt acccttggat caaatcaaag gtattcgcgt gacgcgctgc
    20821 ggtacacgac atccggcaga tcaggtgatc ccgcagcaag atccgcgcgg caatacgctg
    20881 tactggattg gcccgccggg cggtaaatgt gatgccggtc cggggaccga ttttgctgcg
    20941 gtagatgagg gctatgtctc catcacgccg ctgcatgtgg atttaactgc gcatagcgcg
    21001 caagatgtgg tttcagactg gttaaacagc gtgggagttg gcacgcaatg gtaagcagac
    21061 gcgtacaagc acttctggat caattacgtg cgcaaggtat tcaggatgag caggtgctga
    21121 atgcacttgc cgccgtgccg cgtgaaaaat tcgttgatga agcgtttgaa caaaaagcct
    21181 gggacaatat cgccttgccg ataggtcagg ggcagacaat ttcgcagccg tatatggtgg
    21241 cgcgaatgac cgaattactc gagctgacgc cgcagtcgcg ggtgctggaa attggcactg
    21301 gttcgggata tcaaacggca atcctggcgc atcttgtcca gcatgtttgc tcggttgaac
    21361 ggattaaagg cttgcagtgg caggcacgtc gccgcctgaa aaatcttgat ttacataatg
    21421 tttcaacccg tcatggtgat ggatggcaag gttggcaggc acgtgcgccg tttgacgcta
    21481 tcattgttac ggcggcaccg ccggaaattc caactgcgct aatgacgcag ctggacgaag
    21541 gcgggattct cgtcttaccc gtaggggagg agcaccagta tttgaaacgg gtgcgtcgtc
    21601 ggggaggcga atttattatc gataccgtgg aggccgtgcg ctttgtccct ttagtgaagg
    21661 gtgagctggc ttaaaacgtg aggaaatacc tggatttttc ctggttattt tgccgtaggt
    21721 cagcgtatcg tgaacatctt ttccagtgtt cagtagggtg ccttgcacgg taattatgtc
    21781 actggttatt aaccaatttt tcctggggga taaatgagcg cgggaagccc aaaattcacc
    21841 gttcgccgca ttgcggcttt gtcactggtt tcgctatggc tggcaggctg ttctgacact
    21901 tcaaatccac cggcaccggt cagctccgtt aatggcaatg cgcctgcaaa tactaattct
    21961 ggtatgttga ttacgccgcc gccgaaaatg gggacgacgt ctacagcgca gcaaccgcaa
    22021 attcagccgg tacagcagcc acaaattcag gctactcaac aaccgcaaat ccagccagtg
    22081 cagccagtag ctcagcagcc ggtacagatg gaaaacggac gcatcgtcta taaccgtcag
    22141 tatgggaaca ttccgaaagg cagttatagc ggcagtacct ataccgtgaa aaaaggcgac
    22201 acacttttct atatcgcctg gattactggc aacgatttcc gtgaccttgc tcagcgcaac
    22261 aatattcagg caccatacgc gctgaacgtt ggtcagacct tgcaggtggg taatgcttcc
    22321 ggtacgccaa tcactggcgg aaatgccatt acccaggccg acgcagcaga gcaaggagtt
    22381 gtgatcaagc ctgcacaaaa ttccaccgtt gctgttgcgt cgcaaccgac aattacgtat
    22441 tctgagtctt cgggtgaaca gagtgctaac aaaatgttgc cgaacaacaa gccaactgcg
    22501 accacggtca cagcgcctgt aacggtacca acagcaagca caaccgagcc gactgtcagc
    22561 agtacatcaa ccagtacgcc tatctccacc tggcgctggc cgactgaggg caaagtgatc
    22621 gaaacctttg gcgcttctga ggggggcaac aaggggattg atatcgcagg cagcaaagga
    22681 caggcaatta tcgcgaccgc agatggccgc gttgtttatg ctggtaacgc gctgcgcggc
    22741 tacggtaatc tgattatcat caaacataat gatgattacc tgagtgccta cgcccataac
    22801 gacacaatgc tggtccggga acaacaagaa gtgaaggcgg ggcaaaaaat agcaaccatg
    22861 ggtagcaccg gaaccagttc aacacgcttg cattttgaaa ttcgttacaa ggggaaatcc
    22921 gtaaacccgc tgcgttattt gccgcagcga taaatcggcg gaaccaggct tttgcttgaa
    22981 tgttccgtca agggatcacg ggtaggagcc accttatgag tcagaatacg ctgaaagttc
    23041 atgatttaaa tgaagatgcg gaatttgatg agaacggagt tgaggttttt gacgaaaagg
    23101 ccttagtaga agaggaaccc agtgataacg atttggccga agaggaactg ttatcgcagg
    23161 gagccacaca gcgtgtgttg gacgcgactc agctttacct tggtgagatt ggttattcac
    23221 cactgttaac ggccgaagaa gaagtttatt ttgcgcgtcg cgcactgcgt ggagatgtcg
    23281 cctctcgccg ccggatgatc gagagtaact tgcgtctggt ggtaaaaatt gcccgccgtt
    23341 atggcaatcg tggtctggcg ttgctggacc ttatcgaaga gggcaacttg gggctgatcc
    23401 gcgcggtaga gaagtttgac ccggaacgtg gtttccgctt ctcaacatac gcaacctggt
    23461 ggattcgcca gacgattgaa cgggcgatta tgaaccaaac ccgtactatt cgtttgccga
    23521 ttcacatcgt aaaggagctg aacgtttacc tgcgaaccgc acgtgagttg tcccataagc
    23581 tggaccatga accaagtgcg gaagagatcg cagagcaact ggataagcca gttgatgacg
    23641 tcagccgtat gcttcgtctt aacgagcgca ttacctcggt agacaccccg ctgggtggtg
    23701 attccgaaaa agcgttgctg gacatcctgg ccgatgaaaa agagaacggt ccggaagata
    23761 ccacgcaaga tgacgatatg aagcagagca tcgtcaaatg gctgttcgag ctgaacgcca
    23821 aacagcgtga agtactggca cgtcgattcg gtttgctggg gtatgaagcg gcaacactgg
    23881 aagatgtagg tcgtgaaatt ggcctcaccc gtgaacgtgt tcgccagatt caggttgaag
    23941 gcctgcgccg tttgcgcgaa atcctgcaaa cgcaggggct gaatatcgaa gcgctgttcc
    24001 gcaagtaagt aagcatctgt cagaaaggcc agtctcaagc gaggctggcc tttttctttt
    24061 gtttgggaag cttgtgtttt tgtcgcctgg ataagacacg tcagcgtcct acccggcaat
    24121 cagcattcta tgtgctcatc atcttgcgca ccaactgcat aaacagctcc tgctcttctc
    24181 cactcaaacg ccccaaaaat tcctcatcca cgctgtcacc aataggtatc gccgcagcaa
    24241 gtagcttttc tccttcggca gtcagccaga caaagcgtcg ccgcttgtca gcgacgtcat
    24301 gttcacgtct gactaagcca cgattttcca ttcttgccaa catttctgct agcgttgctt
    24361 tggtgctgac tgctgcttct atcagtgcca cctgttcaat accaggttta tcagcaatgg
    24421 cgcgcatcac cgcgtactgt ggtttggtca ggtcaggtaa ctcgtgctgc cagcgagccg
    24481 tgtgctgctg aaaaagctgt cgtaactgat ggaacgcttt atttcgtaac gccatgtaaa
    24541 ctccgggcta attctctgtc gctatcatag cggtttttta gtgacgaaga agatgttgtt
    24601 acttttcaat ccttgccgct gtggaaatga aggagtatgt taataataac gttcgtatac
    24661 gaataattaa gagaataagc aatgaaactg atcgtcggga tgacaggggc taccggtgcg
    24721 cctcttggtg tggcattact gcaagcgctg cgtgagatgc cgaatgtcga gactcatctg
    24781 gtgatgtcga agtgggcgaa aaccaccatt gaactggaaa cgccttacag cgctcgcgat
    24841 gttgctgccc tcgcagactt cagccataac ccggcggatc aggcggcgat catctcatcc
    24901 ggttcttttc gtaccgacgg catgatcgtt attccgtgca gtatgaaaac gctcgccggt
    24961 atccgcgctg gttacgccga tggcctggta gggcgcgcgg cggacgtcgt gctcaaagaa
    25021 ggccgcaaac tggtgctggt gccgcgtgaa atgccgctta gcaccatcca tctcgaaaat
    25081 atgctcgcac tttcacgcat gggcgtggcg atggtgccgc cgatgcctgc cttttataac
    25141 catcccgaaa cggtagatga cattgtccac catgtggtag cccgcgtgct ggatcaattt
    25201 ggcctcgaac atccccacgc caggcgctgg caaggattgc cgcaggcccg gaatttttct
    25261 caggagaatg aataatggca tttgatgatt tacgcagctt tttacaggcg cttgatgacc
    25321 acggccagtt actgaaaatc agcgaagaag tgaacgctga gccggatctg gcagcagcag
    25381 ctaacgccac cgggcgtatc ggcgacggcg cgcccgcgct gtggtttgat aatattcgcg
    25441 gctttaccga tgcccgcgtg gcgatgaata ccatcggttc ctggcagaac cacgcgattt
    25501 cccttggcct gccaccaaac accccggtca aaaagcagat tgatgagttt atccgccgct
    25561 gggataactt cccgattgcc ccggagcgcc gcgccaatcc agcctgggcg cagaacaccg
    25621 tcgatggcga agagatcaac ctgttcgata ttctgccgct gtttcgcctg aatgatggcg
    25681 atggcggttt ctatctcgac aaagcgtgcg tggtttcccg cgatccgctc gacccggata
    25741 acttcggcaa gcagaacgtc ggcatctacc gcatggaagt gaagggcaag cgtaagctcg
    25801 gcctgcaacc ggtgccgatg cacgatatcg ccctgcatct gcataaagca gaagagcgcg
    25861 gtgaagatct gccgattgcg atcacgctcg gtaacgatcc gatcatcacg ctgatggggg
    25921 ccacgccgct gaaatacgat cagtctgagt atgaaatggc aggcgcgctg cgtgaaagcc
    25981 cgtacccgat cgccaccgct ccgctgaccg gttttgatgt gccgtggggt tcagaagtga
    26041 tcctcgaagg ggttatcgaa agccgtaaac gcgaaatcga agggccgttc ggtgagttta
    26101 ccgggcacta ctccggtggg cgcaacatga ccgtggtgcg catcgataaa gtctcttatc
    26161 gcagcaaacc gattttcgaa tcactctatc tcggtatgcc gtggaccgaa atcgactacc
    26221 tatgactagg taatttattc aagataaccg ggaagagatt aaaaaagacg ggaatagatt
    26281 ggaatcaaca agttaggaac tcaaggtttt caagataaag cgagtccaaa aatgttaaac
    26341 ggttcgatat caataaaaca ttcaagataa ctcacaggtt cttacaatag cctttaaaga
    26401 ccgttcaaaa acgattcagg tgtttattat agcgctgatg ggtgcaagcg ccaaaaccaa
    26461 tgtaatttta gcacagctag gcacttgcac tgggttatgc gctggctgct ttctgaggta
    26521 gatttaagtg atcgccctta gcttcggaca acgcgtaaat ctctctaacc ttttcgtctg
    26581 gcaaattacc aaggcttaac gctatggctc tacgaactcc aactagagcg tgcaactctt
    26641 ggatgtgctc atccagaaga atgagtaatt gctccgctcc ttttctccca agaacatcag
    26701 ctacttgttg tttttcttgt ggtttaagaa atgacaagaa cattagaatt tgagcatcaa
    26761 cattatccgc attctctttt ttgggttctt tggaatcttt tagttcaaca tcaccctctc
    26821 ccgtcatgag ccattccaag gtaacatttt cgctttctgc tatctttttt aactgatacg
    26881 ggcgtggtat cggtgcgtaa tcccgcctgt tgtaataatt ttttaaagtg ccttcattga
    26941 tcccccatgc ccgagcagcc gcatttcttg aagggtatcg ttctactaac ctaaagattc
    27001 tttcgataat cggttctttt ccatcggcag gaaaagaacc ttggggtgct tttctcattc
    27061 ttggttcctt tctatctaat tcattgataa ataataaaat taaaaaataa accataaaaa
    27121 caccaaagaa ccctttgcag ggaatctttg gtatcttaat ctttatccat acggatatcc
    27181 gaccggatat ccgcgcgggt aattttgtag gttaaaggaa agatgagaca aagagaagat
    27241 agtgaccgcg attggccttc gctgaagatt cagctggcgc tcaaagataa cgggtggtct
    27301 tccttccggg agctttcact ccatgctggt tatgctcagg attcactgaa agcggtagtt
    27361 cgcacaccaa acaaaaaata cgaacgcgta atttctgacg ctataggtgt accgccagaa
    27421 atcatctggc ccagccggta tcgtaacgat ggatttatca ggaaggcgtc gtgatatgca
    27481 gtcgtacgtt accgtaaatg acctgctcgg cgtaccagga atgccagcca ccacaaaagg
    27541 catccgacag gcgttacagc gtttttctcg tgatcttggt gacgttagtc gccgtcggga
    27601 aggcacaaaa gccattgagt accacattga ttgtctgcct gaaataacac gaaaggcact
    27661 gcgtgaacgc tatgtggaac agctggtggc tacagagaac aatgtttctg aagtgaaagc
    27721 tgttaccaga aaaacacgca atcctgacgc tgtccaggca atcgaagcat atcgcggttc
    27781 tccacaactg atggaagaac gcctgaatgc gctgaccgaa aaccagcgct gggtatctga
    27841 agcaagagct gcgctggtgg tggaagtgct gaagctggaa agcgccggta accccgggcg
    27901 actgaaagcc attaactttc ttgttgaaaa agcccgtaaa ggtgagctgc cggagcgcct
    27961 gcaacaggcc gcagttaacg ccaatgcaaa acgtggcgct aatcgtacta tcagccgcga
    28021 tccactttac cagtgggttc tgaaatacaa ccagtcgcag aacgccgccg aacggctgct
    28081 gttactggca ccgggaaaac gcgacgaaat aaaaccggaa gaaatcagct ggctgcctga
    28141 atttctggcg cagtatcgcc aggtgaacgg cagacccatg tcagaagcct acgaggattt
    28201 tgtcgcagaa tggcagcgtc gccatgcgga cgaaccgtac atgctggagg tgatgccttc
    28261 ctatgatgtt gtccggtatg ccatgaaaaa actgccggaa gtggtgaaac aaaaagggcg
    28321 cgttaccggc agtgaatacc gtcagcttga aggattcacc cgccgcgact ggacagctat
    28381 gccggtgaat tatgtctgga ttggtgacgg tcacggtatg aagctgaaat gtgcgcatcc
    28441 gatccacggg cgtccattct caccggaagt cacgtttgtg atcgatggtg gaacacggtt
    28501 tgttgttggc tggagtcttg accttgccga aaacgtgttc gccgttgccg gtgcgataca
    28561 gcacggcatt cgcaaccacg gaaaaccgtt cctgtattac tcggataacg gttccggtga
    28621 aacggctgac atgctggaca aagaggtcgt gggtattctg ccacgcctgg ggattaaaca
    28681 ccctaccggt attgccggta atccacaggg acgcggcatt attgaacgcc tgaaccggac
    28741 actgccgatg cgtatagcca ggaggtatcg cacctatttt ggcaagggtg ctgaccggga
    28801 atcgttgcgt gtacttaacc gtgatctgcg ttcagcgttt aacgccctgc aacaggacaa
    28861 gcctctgaac gaccgccaga aagcggcaat gcgcgagctg ccatcgtggg cggagcttat
    28921 tgaggctatt cgtgaaggcg tggaatggta caacaaccgc ccgcattctg agctgccgat
    28981 gaaaccgaac ggacggcatt acagcccgac agagttcaga aaaaaacgcc aggcagaaga
    29041 agacaccgaa attgagtggc tgagtgatct tgagctgcgc gacatgttcc gcccaatggt
    29101 ggaacgaccg gtaagacgct gcgaaattca gtggctgaac aatatttact acgcgccgga
    29161 gttgcgtgac gaacacggtc gcaaagtgct tatcagttat gacattcatg acgccgagcg
    29221 aattacggta cgtcgcaagg acggcagctt tatctgtgag gcgatatgga acggcaataa
    29281 acgcgcagcc tttgctgtca gtgctgaata tcacaaacaa cagcagcgta ttaaaggaat
    29341 gcgcaaacgc gctgaggaaa aaattcgtga tgctgaagat gaaggtattc agattctgga
    29401 acacaaacag gctgaaccct ggctgagtaa tgtttatcgc ccggttggta atgtggtggc
    29461 agttcagcaa ccggaatatg aagaagagca tgacgaagaa tttgaacgcg atttccggct
    29521 ggggatgcaa aaactttttg ccatgcagga agaagatgat ccgctggcct gaataaaa
//
LOCUS       DAJTYH010000045        29341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTYH010000045 DAJTYH010000000
VERSION     DAJTYH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            84..1580
                     /gene="ravA"
                     /locus_tag="OO609_001963"
     CDS             84..1580
                     /gene="ravA"
                     /locus_tag="OO609_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6633391.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1574..3025
                     /gene="viaA"
                     /locus_tag="OO609_001964"
     CDS             1574..3025
                     /gene="viaA"
                     /locus_tag="OO609_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6633392.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3030..4022)
                     /gene="asnA"
                     /locus_tag="OO609_001965"
     CDS             complement(3030..4022)
                     /gene="asnA"
                     /locus_tag="OO609_001965"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6633393.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4174..4632
                     /gene="asnC"
                     /locus_tag="OO609_001966"
     CDS             4174..4632
                     /gene="asnC"
                     /locus_tag="OO609_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6633394.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4722..5165
                     /gene="mioC"
                     /locus_tag="OO609_001967"
     CDS             4722..5165
                     /gene="mioC"
                     /locus_tag="OO609_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6633395.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5544..7433
                     /gene="mnmG"
                     /locus_tag="OO609_001968"
     CDS             5544..7433
                     /gene="mnmG"
                     /locus_tag="OO609_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6633396.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7497..8120
                     /gene="rsmG"
                     /locus_tag="OO609_001969"
     CDS             7497..8120
                     /gene="rsmG"
                     /locus_tag="OO609_001969"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6633397.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8737..9117
                     /gene="atpI"
                     /locus_tag="OO609_001970"
     CDS             8737..9117
                     /gene="atpI"
                     /locus_tag="OO609_001970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6633398.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9126..9941
                     /gene="atpB"
                     /locus_tag="OO609_001971"
     CDS             9126..9941
                     /gene="atpB"
                     /locus_tag="OO609_001971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6633399.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9988..10227
                     /gene="atpE"
                     /locus_tag="OO609_001972"
     CDS             9988..10227
                     /gene="atpE"
                     /locus_tag="OO609_001972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6633400.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10289..10759
                     /gene="atpF"
                     /locus_tag="OO609_001973"
     CDS             10289..10759
                     /gene="atpF"
                     /locus_tag="OO609_001973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6633401.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10774..11307
                     /gene="atpH"
                     /locus_tag="OO609_001974"
     CDS             10774..11307
                     /gene="atpH"
                     /locus_tag="OO609_001974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6633402.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11320..12861
                     /gene="atpA"
                     /locus_tag="OO609_001975"
     CDS             11320..12861
                     /gene="atpA"
                     /locus_tag="OO609_001975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6633403.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12912..13775
                     /gene="atpG"
                     /locus_tag="OO609_001976"
     CDS             12912..13775
                     /gene="atpG"
                     /locus_tag="OO609_001976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6633404.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13802..15184
                     /gene="atpD"
                     /locus_tag="OO609_001977"
     CDS             13802..15184
                     /gene="atpD"
                     /locus_tag="OO609_001977"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6633405.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15205..15624
                     /gene="atpC"
                     /locus_tag="OO609_001978"
     CDS             15205..15624
                     /gene="atpC"
                     /locus_tag="OO609_001978"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6633406.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15975..17345
                     /gene="glmU"
                     /locus_tag="OO609_001979"
     CDS             15975..17345
                     /gene="glmU"
                     /locus_tag="OO609_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6633407.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17507..19336
                     /gene="glmS"
                     /locus_tag="OO609_001980"
     CDS             17507..19336
                     /gene="glmS"
                     /locus_tag="OO609_001980"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6633408.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19498..20319
                     /locus_tag="OO609_001981"
     CDS             19498..20319
                     /locus_tag="OO609_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6633409.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20306..22414
                     /locus_tag="OO609_001982"
     CDS             20306..22414
                     /locus_tag="OO609_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6633410.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22411..24078
                     /locus_tag="OO609_001983"
     CDS             22411..24078
                     /locus_tag="OO609_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6633411.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24081..25607
                     /locus_tag="OO609_001984"
     CDS             24081..25607
                     /locus_tag="OO609_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6633412.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25608..27224
                     /locus_tag="OO609_001985"
     CDS             25608..27224
                     /locus_tag="OO609_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6633413.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27280..27432)
                     /locus_tag="OO609_001986"
                     /pseudo
     CDS             complement(27280..27432)
                     /locus_tag="OO609_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27455..27832)
                     /locus_tag="OO609_001987"
     CDS             complement(27455..27832)
                     /locus_tag="OO609_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633414.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28242..28613)
                     /locus_tag="OO609_001988"
     CDS             complement(28242..28613)
                     /locus_tag="OO609_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633415.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28674..29171)
                     /locus_tag="OO609_001989"
     CDS             complement(28674..29171)
                     /locus_tag="OO609_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633416.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29200..29341)
                     /locus_tag="OO609_001990"
                     /pseudo
     CDS             complement(29200..29341)
                     /locus_tag="OO609_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase"
BASE COUNT         7572 a         6850 c         7552 g         7367 t
ORIGIN      
        1 tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac gcagaacttt
       61 tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa agaatttccc
      121 gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc cgcttgtgtt
      181 tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt attgccaaaa
      241 gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt gaatatctga
      301 tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag gcgctaaaag
      361 atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa atcgtctttc
      421 tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc gccattaacg
      481 agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta ctggtggcgg
      541 cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac cgcatgctga
      601 ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg ctgaccagtc
      661 aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat gaagaatatg
      721 aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt gagctgattt
      781 ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg gatcgtcgct
      841 ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc agtgctgttg
      901 ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa agcctgaatt
      961 tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag caagggatgt
     1021 tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa caaagcgata
     1081 aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag cagtatcaac
     1141 ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg ttaaaactgc
     1201 atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag tggctgagta
     1261 aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa ctgaatctgg
     1321 aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc agtacgctgg
     1381 cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa ctggaagagc
     1441 ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa tgtctgttta
     1501 tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt gcacagattc
     1561 gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg tcagcgaaga
     1621 gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg cggtcttctt
     1681 tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct ggcgtgaggc
     1741 gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag aggtgatgtg
     1801 ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac cacagatcct
     1861 ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt tggttgatgc
     1921 taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc gtttaagtct
     1981 gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg aacaactgtt
     2041 aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc tcgcagataa
     2101 caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac gtggcgacta
     2161 tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga aacgcctggc
     2221 agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg cgcagatgga
     2281 aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg atggtctgca
     2341 acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag ggataacgga
     2401 actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct atcgcctgca
     2461 cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag attacgatga
     2521 acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg gcggctttaa
     2581 tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg ctgaaaaccg
     2641 gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt caggcccaca
     2701 aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca ccgatcttgc
     2761 cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg atgccgatgc
     2821 ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga gtaaagtgaa
     2881 agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg cacacggcaa
     2941 acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga tgcgaagccg
     3001 cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct ctcgcgaaca
     3061 gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag cagcagcata
     3121 gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc gcgcagcagc
     3181 gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag ctgatgcttc
     3241 agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc ttccagtacc
     3301 gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga cggggtgctc
     3361 cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa cttgccgcca
     3421 atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg ccctttggca
     3481 tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat ctgatccggc
     3541 aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt aattcccgcc
     3601 cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc gcccattacg
     3661 cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg gtcttcatcg
     3721 gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc gtgttgccct
     3781 aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc atcaggcaga
     3841 gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg cgtgccatcc
     3901 cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc ttccagttgg
     3961 cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta agcggttttc
     4021 attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg acacacatgc
     4081 aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga cggctaaaat
     4141 agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat cgacaatctg
     4201 gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc cgaactggcg
     4261 aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat gaagcaggcg
     4321 gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta tgacgtaggc
     4381 tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct ggcaaagctg
     4441 gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag catctttata
     4501 aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa gatccaaaca
     4561 attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat catgcgtacc
     4621 atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc ccaacgcgtt
     4681 cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat atcactctta
     4741 tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct gaaaagctgg
     4801 aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat ttatctgcct
     4861 cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg gacaaccttt
     4921 ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc cgctttggca
     4981 caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat aaactcgagg
     5041 cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc aacattcttg
     5101 atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt aatttactca
     5161 aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc ttggatcaac
     5221 cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa cccctcattc
     5281 tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat cctttccagg
     5341 ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc taataagaga
     5401 tcacaataga acagatctct aaataaatag atcttctttt taatacccag gatcccaggt
     5461 ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc cattttcata
     5521 ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc atcatcattg
     5581 gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt caacagactc
     5641 tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg gcgatcggcg
     5701 gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg atggcgaaag
     5761 cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga ccggcggttc
     5821 gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt acggcgctgg
     5881 agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt gtcgaaaacg
     5941 atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa gctgtggtgc
     6001 tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat tacagcggtg
     6061 gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa ctgccgctgc
     6121 gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc atcgacttta
     6181 gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt atgggcaatg
     6241 cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag aaaacccatg
     6301 atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc gaaggtgtcg
     6361 gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac agaaatcagc
     6421 atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg aacggtatct
     6481 ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag gggatggaaa
     6541 acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat cctcgcgacc
     6601 tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt cagattaacg
     6661 gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt aacgctgccc
     6721 gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat ctcggcgtac
     6781 tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt acttcgcgcg
     6841 cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact gaaatcggtc
     6901 gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa cttgagaata
     6961 tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg gaagctgcag
     7021 ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt gaagatctgc
     7081 tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt gcccctgcgt
     7141 tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa ggttatatcg
     7201 cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg ctacccgcga
     7261 cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa cttaacgatc
     7321 acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct gcggccatct
     7381 ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca taacgcatta
     7441 aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta atcaccgtgc
     7501 tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat caccagaaaa
     7561 accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac aacctgactt
     7621 cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt gtggtggcac
     7681 cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg ccaggcattc
     7741 cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt ggtaaacgcg
     7801 tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa ccagtacaga
     7861 gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc cgcgcttttg
     7921 cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa ggccgtttct
     7981 acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa gaatatcagg
     8041 tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat ctggtggtga
     8101 ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac cggttagact
     8161 gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc acgttcactc
     8221 ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt cattagtgaa
     8281 aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa acattattaa
     8341 aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt atctgttttt
     8401 agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa tatgctgtgc
     8461 gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta cgcgttattt
     8521 acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc actggaaaaa
     8581 tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg ggcgcaccgt
     8641 ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt atacgacacg
     8701 cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct cgtgagtcga
     8761 aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag tggattgctg
     8821 ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct ggcagtcttt
     8881 ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac accagcgaaa
     8941 ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc gatgttggtg
     9001 ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat cgttacgtgg
     9061 gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa agggtaaaag
     9121 gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac ctgaataacc
     9181 ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca gccaccttct
     9241 ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg ttcctggttt
     9301 tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt cagaccgcga
     9361 ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat ggcaaaagca
     9421 agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg aatctgatgg
     9481 atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt ctgcctgcac
     9541 tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg ggcgtattta
     9601 tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg aaagagttga
     9661 cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt gaaggggtaa
     9721 gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg tatgccggtg
     9781 agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg atcctgaatg
     9841 tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc ttcatggttc
     9901 tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac actactacgt
     9961 tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct gctgtacatg
    10021 gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat cggcatcctc
    10081 gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct gctgcgtact
    10141 cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt aggtctgggt
    10201 ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga gcaatatcag
    10261 aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc tcggccaggc
    10321 catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc cattaatggc
    10381 agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag aacgagcaca
    10441 taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag cgaaagcgga
    10501 agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg acgaagcgaa
    10561 agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag aaattgaagc
    10621 cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg ctgttgctgg
    10681 cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca tcgtggataa
    10741 acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac ggtagctcgc
    10801 ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga acgctggcag
    10861 gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga gcttctctct
    10921 ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg tgagcaactg
    10981 gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct taacgcgctc
    11041 ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac cgctgaggta
    11101 gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc tgctgcgatg
    11161 gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc tgtaatggct
    11221 ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg tcgtcttgag
    11281 cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa ttccaccgaa
    11341 atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga agctcacaac
    11401 gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct ggccgattgt
    11461 atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact gaacctcgag
    11521 cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga aggcatgaaa
    11581 gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct gggccgtgtg
    11641 gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga cggcttctct
    11701 gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca gccggtacag
    11761 accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg tgaattgatc
    11821 atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat caaccagcgc
    11881 gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac catttctaac
    11941 gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt ggtagcaacc
    12001 gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc aatgggcgaa
    12061 tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc taaacaggct
    12121 gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga agcattcccg
    12181 ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt taacgccgaa
    12241 tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct gaccgcactg
    12301 ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa cgtaatctcc
    12361 attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat tcgtcctgcg
    12421 gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa gatcatgaaa
    12481 aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc agcgttctct
    12541 cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg tcagaaagtg
    12601 accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca gtctctggtt
    12661 ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat tggcagcttc
    12721 gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca agagatcaac
    12781 cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct cgattccttc
    12841 aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg caaggcattg
    12901 aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc gtccagaaca
    12961 cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt aaatcgcagg
    13021 atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt ggtcaccttg
    13081 cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt aaacgcgtgg
    13141 gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt aacctgttca
    13201 aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc gacctcgcaa
    13261 tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt gttgcccagg
    13321 tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta aaagtgatgt
    13381 tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac aaatttatta
    13441 acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca tcagatgatg
    13501 atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag gcgttgctgg
    13561 ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt gaaaacctgg
    13621 ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat ggcggcagcc
    13681 tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt actcaggaac
    13741 tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta gaggatttaa
    13801 gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg aattccctca
    13861 ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg agcgtctggt
    13921 gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa tgggttcctc
    13981 cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg aagtcccggt
    14041 aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg acatgaaagg
    14101 cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct acgaagagct
    14161 gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga tgtgtccgtt
    14221 cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa ccgtaaacat
    14281 gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt ttgcgggcgt
    14341 aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact ccaacgttat
    14401 cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc gtctgcgcgt
    14461 tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg acgttctgct
    14521 gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg cactgctggg
    14581 ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg gcgttctgca
    14641 ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg tatacgtacc
    14701 tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg acgcaaccgt
    14761 ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc cgctggactc
    14821 caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca ccgcgcgtgg
    14881 cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca tcctgggtat
    14941 ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga tccagcgctt
    15001 cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta aatacgtctc
    15061 cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg atcacctgcc
    15121 ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag ccaaaaaact
    15181 ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt cgtcagcgca
    15241 gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag cgaaggtgaa
    15301 ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg tatgattcgc
    15361 atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat tcttgaagtg
    15421 cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga tctcgacgaa
    15481 gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc tcacggcgac
    15541 gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct gcgcgttatc
    15601 gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca gtctggaaac
    15661 aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga tcctgctgat
    15721 ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa aacatccacc
    15781 tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg acgatggtcg
    15841 aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc tctgtccatt
    15901 tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt tctcaaatta
    15961 cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc gcaggcaaag
    16021 gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg aaagcgatgg
    16081 ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac ctggtgtacg
    16141 gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac tgggtgcttc
    16201 aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc tttgccgatg
    16261 atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa acactccagc
    16321 gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa ctggatgatc
    16381 cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt gttgagcaca
    16441 aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt ctgattgcca
    16501 acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct cagggcgaat
    16561 actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc gtcgccgttc
    16621 atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc tcccgtctgg
    16681 agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt atgctgcgcg
    16741 atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt gaaattgata
    16801 ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc ggcaccggtt
    16861 gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat accgttgtgg
    16921 aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg cgtcctggtg
    16981 ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa gcgcgtctgg
    17041 gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt ggcgataacg
    17101 ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa tttaagacca
    17161 ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg gtaacagtag
    17221 gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc gaaaatgcat
    17281 tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg gtaaagaaaa
    17341 agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa tctccctccc
    17401 acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc ccgaaaaaac
    17461 gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt gtggaattgt
    17521 tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac gtcgtctgga
    17581 ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc atatgactcg
    17641 cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac atcctctgca
    17701 tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt cagaagctaa
    17761 cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca tcgaaaacca
    17821 tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg aaaccgacac
    17881 cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc tgcgtgaggc
    17941 tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca tggactcccg
    18001 tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg gcctgggtat
    18061 gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc gtcgctttat
    18121 cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca tcttcgataa
    18181 aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg acgcgggcga
    18241 taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga acgcgataaa
    18301 aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc tgggaccgaa
    18361 cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg gtacttctta
    18421 taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc cgtgcgacgt
    18481 cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca gcctgatgat
    18541 caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc tgtcgaaaga
    18601 gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc tggtgcgcga
    18661 atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt ccactaaagc
    18721 attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc gcctgaaagg
    18781 tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc cgagccgtat
    18841 tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt tctctgacaa
    18901 acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg aaggcgcatt
    18961 gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg agctgaaaca
    19021 cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac cgaacaacga
    19081 actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg gtcaattgta
    19141 tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca tcatcgagat
    19201 gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc agctgctggc
    19261 ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc tggcaaaatc
    19321 ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc ggacaataaa
    19381 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
    19441 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
    19501 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
    19561 ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt tccttcttca
    19621 ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt gctatctgac
    19681 ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga tatacgcgag
    19741 cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg tattaagcat
    19801 cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga ctgcaaagat
    19861 ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca agacgagcgt
    19921 accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat tccttggttc
    19981 atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg gctttattca
    20041 gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt ggcccatatc
    20101 ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga tattgcttat
    20161 gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa tggttttatt
    20221 aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg tattagccaa
    20281 gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc tatttgataa
    20341 tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc aaataagcgc
    20401 tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt tagatgaagg
    20461 ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc cgtctgtaga
    20521 ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta ttattaatag
    20581 taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg tccaagaaca
    20641 taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc gtggtcaaac
    20701 gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa gacgttcagc
    20761 gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag gaaccaaggt
    20821 aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt taaatcaaaa
    20881 aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc agtattttcc
    20941 tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt atgatcgaga
    21001 ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata aaaaagacgt
    21061 acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc gttatgagat
    21121 tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa aaatcatagg
    21181 aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg gcttttatat
    21241 cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg cttgctctga
    21301 caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact ggccgtgtgt
    21361 aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc aggtcgaagc
    21421 cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg gcgatgctaa
    21481 aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct ttgcacctgg
    21541 cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt tagatgcatc
    21601 tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca gaaataacca
    21661 tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac cgtctattcc
    21721 tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg ctgtggagca
    21781 agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt catttggcgt
    21841 taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt ggttgcagcg
    21901 gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag tgctggttga
    21961 tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta atctgacgga
    22021 acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac aagcacaaga
    22081 aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg agcttgaggc
    22141 gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta ctgagcccaa
    22201 atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct cggagcgtaa
    22261 aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag ttattccttt
    22321 caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg tgcctgaatt
    22381 atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca agcagtttat
    22441 cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt accaccatta
    22501 caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac ttcctctgac
    22561 ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga tgactatttt
    22621 cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat tcgaggtggc
    22681 tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa tggttatgag
    22741 cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc tacggcacaa
    22801 agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca tcgtattcta
    22861 gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt ggtgtatttg
    22921 aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt tttcagagcg
    22981 ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg tcatggtata
    23041 gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg gttgttggtt
    23101 attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga gatgctgaac
    23161 ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg tacccctaaa
    23221 gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg gtttggagct
    23281 atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga gtggatcgct
    23341 tttacggata atctttggca attacagctt ttacaacgca aagatgcgct gttatcggat
    23401 gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt agtaaaactt
    23461 tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac cgctggttta
    23521 ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga ggcattacgc
    23581 tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga gattgataaa
    23641 cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc agaagaaaaa
    23701 gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct gaaagaggat
    23761 tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa tccaacgatg
    23821 acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga aacggtagtg
    23881 tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt agtcaagccc
    23941 agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca acgccaacct
    24001 gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat ggcgagctta
    24061 tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat gagctgattt
    24121 atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag cagctgttgg
    24181 atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg catttaggtg
    24241 tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt atttatgagc
    24301 ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac gaagctattc
    24361 ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc gctgcttcta
    24421 gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt cagctaaata
    24481 ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca tattgtccaa
    24541 aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat caattttggg
    24601 ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct caattaacag
    24661 cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag ctttccccaa
    24721 gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg ctaaccaaaa
    24781 gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt agtgatgagg
    24841 cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg ctgaaaagta
    24901 tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg tggcaagcct
    24961 tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt actgagcact
    25021 ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat ttatctgtta
    25081 agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg gcaagacagt
    25141 ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat tggctagttc
    25201 actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt gattggaacc
    25261 aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta gatagtagcc
    25321 ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac ggaacctctc
    25381 ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt tactcagaga
    25441 gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt aaacaggaag
    25501 atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa gagcggataa
    25561 ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg gttaggctag
    25621 ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt aactcgggtc
    25681 ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa tggactcgat
    25741 ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca aagcaaataa
    25801 ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc aaaataatcg
    25861 attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg cagagttcat
    25921 ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca cagttagagc
    25981 tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg agcagtaccg
    26041 ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta gagataagga
    26101 tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta gtgcagcttt
    26161 tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt tttaggcact
    26221 atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc tttgaccctc
    26281 cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag gataaggatt
    26341 atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc acaacagcta
    26401 ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag cacatagcgg
    26461 tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag gaaacagcca
    26521 atatagacac agacacacga gtcatagagg ctgagccgac atggataagt tttagtagac
    26581 ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt ttagaaaaag
    26641 aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca cacttaggtg
    26701 gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac aactctattt
    26761 ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact aaatttgcat
    26821 gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc cgattacatc
    26881 tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt aatggcagtg
    26941 aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct accaaagtgt
    27001 tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg cgtggagtgg
    27061 tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga caagacgggc
    27121 atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga gaggatatgg
    27181 aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat gactgaaaga
    27241 gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc catttaaacg
    27301 gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga tcggcatcgt
    27361 ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg attcgtccgt
    27421 agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg tggttttgag
    27481 gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg gcgacatgtt
    27541 tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt gcttggacga
    27601 ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga aatccaatat
    27661 cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa gcttcccagg
    27721 ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca tcaacttttt
    27781 tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac acgtgcgttt
    27841 cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt cccaaagcat
    27901 ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt gatatttgaa
    27961 acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc ttgtaaatat
    28021 cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta tagaaatcgt
    28081 cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca tcgccatacc
    28141 ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga gcttgtacgc
    28201 aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc catccattcg
    28261 gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta tgttgcctgc
    28321 accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc ccacttgccc
    28381 acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc ctcctttaac
    28441 tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc gacatagtct
    28501 ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt gtcatacatg
    28561 acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga cactagtggt
    28621 tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc aggctagttg
    28681 cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg gccatttatg
    28741 acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg cccccctttt
    28801 ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc attgttggca
    28861 atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag cctacgattt
    28921 ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc agagttgtcg
    28981 taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct tggagaaatg
    29041 tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa aacaattggc
    29101 aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac caccttcaac
    29161 agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc cgcgaagcgg
    29221 cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc
    29281 acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttgtcca
    29341 a
//
LOCUS       DAJTYH010000046        29311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTYH010000046 DAJTYH010000000
VERSION     DAJTYH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(84..884)
                     /gene="otsB"
                     /locus_tag="OO609_001991"
     CDS             complement(84..884)
                     /gene="otsB"
                     /locus_tag="OO609_001991"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6633417.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1051..2037)
                     /gene="araH"
                     /locus_tag="OO609_001992"
     CDS             complement(1051..2037)
                     /gene="araH"
                     /locus_tag="OO609_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6633418.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2052..3566)
                     /gene="araG"
                     /locus_tag="OO609_001993"
     CDS             complement(2052..3566)
                     /gene="araG"
                     /locus_tag="OO609_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6633419.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3636..4625)
                     /gene="araF"
                     /locus_tag="OO609_001994"
                     /pseudo
     CDS             complement(3636..4625)
                     /gene="araF"
                     /locus_tag="OO609_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5422..5925
                     /locus_tag="OO609_001995"
     CDS             5422..5925
                     /locus_tag="OO609_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6633420.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6005..6256)
                     /locus_tag="OO609_001996"
     CDS             complement(6005..6256)
                     /locus_tag="OO609_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6633421.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6371..6457)
                     /gene="azuC"
                     /locus_tag="OO609_001997"
     CDS             complement(6371..6457)
                     /gene="azuC"
                     /locus_tag="OO609_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6633422.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            6720..7043
                     /locus_tag="OO609_001998"
     CDS             6720..7043
                     /locus_tag="OO609_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6633423.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7214..7711
                     /gene="ftnA"
                     /locus_tag="OO609_001999"
     CDS             7214..7711
                     /gene="ftnA"
                     /locus_tag="OO609_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6633424.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(7748..7987)
                     /locus_tag="OO609_002000"
     CDS             complement(7748..7987)
                     /locus_tag="OO609_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6633425.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8177..9388
                     /gene="tyrP"
                     /locus_tag="OO609_002001"
     CDS             8177..9388
                     /gene="tyrP"
                     /locus_tag="OO609_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6633426.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9450..10115)
                     /gene="yecA"
                     /locus_tag="OO609_002002"
     CDS             complement(9450..10115)
                     /gene="yecA"
                     /locus_tag="OO609_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6633427.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10311..10397)
                     /locus_tag="OO609_002003"
     tRNA            complement(10311..10397)
                     /locus_tag="OO609_002003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10361..10363),aa:Leu,seq:taa)
     gene            complement(10410..10483)
                     /locus_tag="OO609_002004"
     tRNA            complement(10410..10483)
                     /locus_tag="OO609_002004"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10449..10451),aa:Cys,seq:gca)
     gene            complement(10538..10613)
                     /locus_tag="OO609_002005"
     tRNA            complement(10538..10613)
                     /locus_tag="OO609_002005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10578..10580),aa:Gly,seq:gcc)
     gene            complement(10765..11313)
                     /gene="pgsA"
                     /locus_tag="OO609_002006"
     CDS             complement(10765..11313)
                     /gene="pgsA"
                     /locus_tag="OO609_002006"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6633428.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11370..13202)
                     /gene="uvrC"
                     /locus_tag="OO609_002007"
     CDS             complement(11370..13202)
                     /gene="uvrC"
                     /locus_tag="OO609_002007"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6633429.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13199..13855)
                     /gene="uvrY"
                     /locus_tag="OO609_002008"
     CDS             complement(13199..13855)
                     /gene="uvrY"
                     /locus_tag="OO609_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6633430.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14151..14327
                     /locus_tag="OO609_002009"
     CDS             14151..14327
                     /locus_tag="OO609_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633431.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14314..14538
                     /locus_tag="OO609_002010"
     CDS             14314..14538
                     /locus_tag="OO609_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6633432.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14571..15191)
                     /gene="sdiA"
                     /locus_tag="OO609_002011"
                     /pseudo
     CDS             complement(14571..15191)
                     /gene="sdiA"
                     /locus_tag="OO609_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15421..16173)
                     /gene="yecC"
                     /locus_tag="OO609_002012"
     CDS             complement(15421..16173)
                     /gene="yecC"
                     /locus_tag="OO609_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6633433.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16170..16838)
                     /gene="tcyL"
                     /locus_tag="OO609_002013"
     CDS             complement(16170..16838)
                     /gene="tcyL"
                     /locus_tag="OO609_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6633434.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(16853..17839)
                     /gene="dcyD"
                     /locus_tag="OO609_002014"
     CDS             complement(16853..17839)
                     /gene="dcyD"
                     /locus_tag="OO609_002014"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6633435.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(17944..18744)
                     /gene="tcyJ"
                     /locus_tag="OO609_002015"
     CDS             complement(17944..18744)
                     /gene="tcyJ"
                     /locus_tag="OO609_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6633436.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(18832..19383)
                     /gene="fliZ"
                     /locus_tag="OO609_002016"
     CDS             complement(18832..19383)
                     /gene="fliZ"
                     /locus_tag="OO609_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6633437.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19429..20148)
                     /gene="fliA"
                     /locus_tag="OO609_002017"
                     /pseudo
     CDS             complement(19429..20148)
                     /gene="fliA"
                     /locus_tag="OO609_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20313..21965)
                     /gene="fliC"
                     /locus_tag="OO609_002018"
     CDS             complement(20313..21965)
                     /gene="fliC"
                     /locus_tag="OO609_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6633438.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22231..23637
                     /gene="fliD"
                     /locus_tag="OO609_002019"
     CDS             22231..23637
                     /gene="fliD"
                     /locus_tag="OO609_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6633439.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23662..24072
                     /gene="fliS"
                     /locus_tag="OO609_002020"
     CDS             23662..24072
                     /gene="fliS"
                     /locus_tag="OO609_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6633440.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24072..24437
                     /gene="fliT"
                     /locus_tag="OO609_002021"
     CDS             24072..24437
                     /gene="fliT"
                     /locus_tag="OO609_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6633441.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24515..26002
                     /gene="amyA"
                     /locus_tag="OO609_002022"
     CDS             24515..26002
                     /gene="amyA"
                     /locus_tag="OO609_002022"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6633442.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26036..26449)
                     /locus_tag="OO609_002023"
     CDS             complement(26036..26449)
                     /locus_tag="OO609_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6633443.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26636..27841
                     /gene="yedE"
                     /locus_tag="OO609_002024"
     CDS             26636..27841
                     /gene="yedE"
                     /locus_tag="OO609_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6633444.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            27838..28071
                     /gene="yedF"
                     /locus_tag="OO609_002025"
     CDS             27838..28071
                     /gene="yedF"
                     /locus_tag="OO609_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6633445.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28178..28848
                     /locus_tag="OO609_002026"
                     /pseudo
     CDS             28178..28848
                     /locus_tag="OO609_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(28888..>29311)
                     /locus_tag="OO609_002027"
     CDS             complement(28888..>29311)
                     /locus_tag="OO609_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6633446.1"
                     /translation="MIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYE
                     MRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADV
                     NGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7282 a         7581 c         6975 g         7473 t
ORIGIN      
        1 cgcctgcggc tttcagtgcc cccagtatgc caacggcaag gccaccggca ctggcggcgt
       61 gctcgtctgg tggtgcaatc cggttagata ctacgactaa acgactcata gtcatcactc
      121 ctgttatttt ctcttttttg ttgtaatgcg gcggttatca tttcaagcca gctccagaca
      181 tccggcacac ccgccagtcg ccatgatgcc tgagttgcac ctgtgccaat ttttactgac
      241 attccgccca gtcggttaac gactgcgaag ccagattcat cggttaaatc atcgcccaga
      301 aatacgggcg ttcgcccgat aaaaggagct tcctgcataa aggctgcaat cgcttcacct
      361 ttactggtac ctctcggttt gatctcgaca acacactttc cctgctgtaa cgccatttgt
      421 ggccagatct gagtaatacg ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc
      481 tgacgataat gcagcgcaaa agccatccct ttcgcctcca gctccgcgcc gggatactga
      541 gcgatgaccg tatgcagttg cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg
      601 atatgtgttt taccattgat gtcacggcgc tccgccccat gcacgcccgc taacgggaag
      661 cgataaggtt ttgccagtgc gtcaagctcc accattgagc gccctgatat caatgccaat
      721 gcaccatcac ttgcggttgc cagtagctgt agtccttgca gaatattgtc aggcacgacg
      781 acctgatcgg gatgcggttt gatttccgcc agcgttccat caagatcaaa aaaccaggca
      841 tatttcgcgg atagttcagg ggtttcggtt aacggttctg tcacccggct ctcctccttc
      901 tcttttctta ttgtgttgcg cagaccgcgc tttacagaca tgtaagtata gacagtgtga
      961 cggaggtcgc catttgcaac gaagaacagc caaccgttgt ggcggctggc tgtggtggga
     1021 aaaaacgtta agttgttgtg gaaaaaagca tcagacagtg cgcttcgctt tttgcttgta
     1081 acggtcgaag atcaccgctg ccagcaggat taagccgcga accacgtact gcgcgaaagg
     1141 agaaatatta agcaggttca tggcgttttc cacggtgcct aaaattaaga tacccgccac
     1201 cacatatgag atttttccga tgccaccttt cagagaaacg ccacctaaaa cgcaggcgga
     1261 gataacaata agctcataac caatcgacgt cattggctgc ccactagtca tacgtgaagc
     1321 cagaataatt ccggctatcg cagataccag gccagagaga acaaagataa taattttggt
     1381 gcgaacaacc ggtacacccg ccagacgcgc agcctcttcg ttcccgccaa tcgccagggt
     1441 attacgacca aaggtggttt tattcagcag caaaccaaag ataatcagac acgcgacggt
     1501 gagccagatt ggcgcaggca gaccgaacca gttagcgtaa ccaagggcaa agaagctttc
     1561 atcttcgata ccgaccgctt taccgtctga aatgatgtac gccagacctc gaacaatctg
     1621 catcgttgcc agtgtcgtga tcagagcatt tattttcagt ttggcgataa caaagccatt
     1681 gaccaggcca cagagaacgc ccagcaacaa acccgctgcc acgccaatcc acaggctttc
     1741 agtcaggttg ataaccaccg ccgtggtgac acccgcacag gcaattacgg aggcgacaga
     1801 aaggtcaaag tcaccggaag caaggcagaa caacatgcca cacgccacca tccccgacat
     1861 ggaaattgcc aggcccaacc ctttcatatt aatgaaggtg gcaaaatttg ggacaaaaat
     1921 ggcacaggcg ataaagagca ccgcaaacac caccagcatg ccgtactgat cccagatacg
     1981 cccgaagctg aatgacgact taggtgcgcc agaccccgat gtagaaacag aagacatcat
     2041 actctcctta ctcaggcaac tgcctggctg actttaggca tcgcaaggct cagtgcctga
     2101 cgctcatctg cctgctcgtg taacaattca ccggcgattt cgccttcccg catcaccaca
     2161 atccggtcgg caacgccgag gacttcaggt aagtcgctgg aggcaaacag caccgccacg
     2221 ccctgcgccg ccagcgcata aattacgtta tatatttcgt gcttagcgcc aacatcaatg
     2281 ccgcgcgtag gttcatccag caaaatgacc ttcatctctt ccgataacca gcggcccaga
     2341 atggcttttt gctgatttcc gcctgagaga ttcatgatca gttgctccgc gcccggcgtt
     2401 ttgatgttga gcgaacgaat gtgctgatcg gcattgtttt cttcccaact gttgttgatt
     2461 acacaaccgc cgagcacatg tttgcgtctg gcactgatgt tgatattgtc gcgaacggag
     2521 tgcacgggaa taatgccttc cgctttgcga gcttccgggc agagcatcat gcctgcggca
     2581 atggcgtggc tcggtttacg aatatcgatc ggctgttggt cgatataaac ctgaccggcg
     2641 gtgatttgcg tcccgccaaa caagcctttc attaattcgc tacgccccgc ccctaccaga
     2701 ccaaacagcc caacaatttc accactgcga accgccagac ttattggcgt acgcacgcct
     2761 agtgctttca cagcatcaag acgcaggcgc tcctcgccat aactacgagg ttgccagccg
     2821 tagatatcgc caatgtcgcg cccgaccatc gcctgcacca gcgcgtcgtg gtcaacctgc
     2881 tgcatatcgg taaaggtttt gacataacgt ccatctttaa agacagtgat ggcatcgctg
     2941 agggcaaata tttcttccat acggtgagaa acgtataaga tcacccgccc ctcttttcgc
     3001 agttcacgaa taacgcggaa aagattgccg atttcgcggg cagaaaggga gctggttggc
     3061 tcatcaaagg cgataatttt agcgttacgc gccagcgctt tggcgatttc aaccatctgc
     3121 cactgaccaa tggcgagata tttcagcggc gtgtccgggt caatatccat accaagatgt
     3181 ttaagttgta aacccgcctc ataattcagc aatgagcgat tcacaatgcc gcctttatgc
     3241 ggcagctggc cgagatagat gttttccgcg acggtcattt ccggcacgag atgcagttcc
     3301 tggtaaataa tcgccacacc cgcgttaagt gctgcggtcg tgtcggaaaa ggacatttcc
     3361 tgcccattaa tcactacaga acccgtggtt ggcgcatagt tgccgctgag gatttttaag
     3421 agagttgatt ttcctgcgcc attttcaccc atcaacgcat gaacctgacc ggcatagcag
     3481 tcaaaactaa tatccgtcag cgccttaacg ccgggaaacg ttttaccgat gccgcgaaat
     3541 gagagatacg gggtagactg ttgcataacg tctccgtgaa tcactggtcg ttcacacccc
     3601 ctctgcatca tgcagagggg aatttttccg gcaaattact taccgcctaa accttttttc
     3661 tccagttctt ctttaaagtt gtcacgcgtg atcagtacca cgtctgtaac ttcggtaaat
     3721 tttggcgttt caacgtcttt tgctacccag ttgtaaagca tttcgctgga tttatagcca
     3781 tgtacgtccg ggcttggcag cagggaaccg tagaagccgg ttgcctgtgc tttagacagt
     3841 tcgcttactg catccacacc gttaatacca ataccgatga tatcggctgc tttaaagccc
     3901 tgaccttcgg ttgcacgtac gccgccaagt acggtgctgt cgttcatacc gacgatcagc
     3961 caatgtttaa cttccggatg ttgaaccagc attgagttgg cagcgtcaaa tgcccccggg
     4021 atgtcgttag atttggtagg tacctgataa atttgttttt ccgggaatcc tgcagctttc
     4081 agtgcgtcca tagaacccgt tgtacggcgg cgggcggtat ccagttcgtt ggcggtaatc
     4141 gccatcactg cgctttcttt gacatcccag ccacgtttct gcatctcttt atacagttcc
     4201 tagccctgac gttcgccaat tttagtcgcc gccatcatca ccagaggaac ggtatccatt
     4261 ggcttacctt tggcgttaac aaactggtca tccactgcaa tgactttcat atcgtagcca
     4321 cgcgctttcg cgacgatggc agagccgagt ttcgggtccg gagtacaaat aacgaaacct
     4381 tttgcgccac tggctgccag gctgtcgatc gcgttcaatg ttttttcgcc atccggcacg
     4441 gcaatcttaa taacctcaaa ccctaaatcc ttcccggctt tatcggcaaa cttccattcg
     4501 gtctggaacc acggctcttc cggttgcttc accagaaaac cgagcttcag gttctccgcc
     4561 atagcggatt gtgacataac ggctgccaga ccaatggctg ccagggcttt agtaaatttg
     4621 tgcatggttc tctccagctt tagtgtcgtt ttgtgtaggg caaaaacgaa tgacattcgt
     4681 taaattaatc ggaaaacaaa gcattacctt ttatctaaaa gataagtgac cgtgttgaca
     4741 tagttttagc gagaaattaa ttctccatag gagagcaata tcacatcgca gaattacagt
     4801 gagaacgtgc ataaatttag cgggaaaaga cataaggaaa agccaatttg tcagacaaat
     4861 tgttgaatgc acagcagatt aatccataag attagcctgg aaatccttgt tgtcttcggt
     4921 acccatgcgg gatgtcttct ttttaaccag tcaataggcc gcattacctg gcgttgagtt
     4981 tttgaaatgg tgtaataacc acaactcaaa gatgtggaaa atgcgcgtca ttcatttcgc
     5041 cattaattat cactgtgctc attaattaac agaacacgta taatgagagc catctcgcaa
     5101 aaatgaaaaa acgttttata aaatcatcac ttcatcatga attcaaattc atcgattaat
     5161 atcaacaaga tacaaaaagc actatcatta aaattcattg catttacatt gatttcacca
     5221 atgaaatgta aaaatatata aacttgatga tttaagcatt ttcttataac cgttcagacg
     5281 ttattcttat ttcagatcat cgtcagaatt gactccacga tcacatttcg gaccggcaga
     5341 aaggaattat tctgcaaaca gtaattatgg tgttttgatt tatcttgcac ctctccactt
     5401 ctggatataa ggatattagg tatggcaacc gctggaatgc ttctcaaact caactctcaa
     5461 atgaaccgcg agttttacgc atccaatctc taccttcacc tgagtaactg gtgttctgaa
     5521 cagagtctga acggcaccgc cactttcctt cgcgcccagg cacagagtaa tgtgacccaa
     5581 atgatgcgca tgtttaactt tatgaagagt gtcggcgcta cccccatcgt taaagccatt
     5641 gatgttccag gtgaaaaact gaactctctg gaagaactgt tccaaaaaac gatggaagaa
     5701 tacgagcaac gttcaagcac tctggcgcag ttagccgatg aagcgaaaga actgaatgat
     5761 gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac agcagcatga tggcctgttg
     5821 ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg cgggtatgtg ccctgtgcag
     5881 accgaccaac atgttctgaa tgtcgtgtca caccagctgc attgatcata catcggcgct
     5941 aatacattgc gccgatgaag tttttgagaa accgctgcct catctgtttg aagcagcggt
     6001 ttttttaatg ggattcaccc tgtggggtaa acttgagttc aatcagcgcg atggcttttt
     6061 ggattgcccg catggtgacc gggtctgcgg cagcgggatg atgagtaaag tcgatattct
     6121 tcagctgact ggacattttt tcacgaactt caacgggcgc gattacatcg agaacatcca
     6181 gaatttgttt gataaccaac tggcaagcta ccacatcaga aaccagttcc tgatcggcat
     6241 tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat agtagcggct
     6301 tcacatcttc agcgatagtc acatccaccg tcatcaggac acaaaaaaac ctgccggagc
     6361 aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt
     6421 tattgaacat acttttcagg attttgcgca gtttcatcgc ggcactccga ccatttgtta
     6481 tacaggtgtt attgtctttg cgcttataat atgacaacca tcacaaaaat caatctttat
     6541 gtgatacaaa tcacataaat acccctttaa tgttataaaa atgataatca aaaaacagcc
     6601 cccctatttc tgacacctac agatggcaag aaatagcgcc tgccaggcgt cttttccggc
     6661 cattgtcgca gcgctgtaac gcgtaaaata gtgctttctc ttactcttct ggctggacca
     6721 tgagacttct gattctgact ctttcattaa taacgctggc ggggtgtacg gttactcgcc
     6781 aggcccatgt gagcgaagtt gacgcggcaa caggtattgt acggttggtt tatgaccagg
     6841 cttttttgca gcatgcccat accgatcgtt atgtcagtcg cgggatcgcc gatcgcgcat
     6901 gccagcagga aggctatacc cacgcgatcc cctttggtca gccagtaggc aactgtagcc
     6961 tttttgcggg ttccttatgt ttgaatacgg aattcacttt gtcatatcag tgccatcatt
     7021 ccgccttccc tgtctttctc taaatatttt aatcagcgag gggatcttcg ctgattaaag
     7081 aaatagcggt aatgcgtttt attcccattc gtatttttaa taattgaagt ttatatttta
     7141 ccttttgcaa ataataaaat aacaaattat agtgacgcca cagcaacaaa tataaccttt
     7201 gtggagcact atcatgctga aaccagaaat gattgaaaaa cttaatgagc agatgaacct
     7261 ggaactgtac tcttcactgc tttatcagca aatgagcgcc tggtgcagct atcatacctt
     7321 cgaaggtgct gccgcgttcc tgcgccgtca cgcccaggaa gagatgacgc atatgcagcg
     7381 tctgtttgat tacctgactg ataccggcaa tttaccgcgt attaataccg ttgaatctcc
     7441 gtttgctgaa tattcctcac ttgatgaatt attccaggaa acctataaac acgaacaatt
     7501 aatcacccag aaaattaacg aactggctca tgctgcaatg accaatcagg actacccaac
     7561 atttaatttc ctgcagtggt atgtttctga gcagcatgaa gaagagaaac tgttcaaatc
     7621 gattattgat aaattaagcc tggcaggcaa aagcggcgaa ggtctgtatt ttatcgacaa
     7681 agaactctct accctcgaca cacaaaacta atgctctccg gcggcaggtt tctgccgccg
     7741 tttcaaatca gtgacggcaa atcttactct gatcggtcga aaaaccttct tctgaaggta
     7801 taaatttgcc ttttgctgcc agaaacgcta ccagctctcc cgctgtcacc ccttctgccg
     7861 agcacgtgtg aaaacgtgcc tgttcaccaa aacgcgcttt aatcgcagct tccagactgg
     7921 catgcgtata ttgctcgcct gattcaatca tcatatttaa cacttcatga ccgtgaatag
     7981 agtccatcgt tcctcctcaa aaaaagccta gcgtagcgat taccgcttat gaagactttg
     8041 cgccagcgca ggactgaatg cttttattgt acatttatat ttacacaata tgtaacgtcg
     8101 gtttgacgaa gcagccgtta tgccttaacc tgcgccgcag atatcactca taaagatcgt
     8161 caggacagaa gaaagcgtga aaaacagaac cctgggaagt gtttttatcg tggcgggaac
     8221 cacaattggc gcaggcatgc tggcaatgcc gctggctgcg gccggtgttg gttttagcgt
     8281 tacgttaatc ttgttgattg ggctttgggc gttgatgtgc tacacggcgc tattactgct
     8341 ggaggtgtac cagcatgttc cggcagatac cggtctgggc acgctggcaa aacgctatct
     8401 gggacgctac ggtcaatggc tgacgggctt cagtatgatg ttcttaatgt atgctctgac
     8461 tgcagcatac atcagcggtg ccggtgaatt gttggcctcc agcatcagcg actggacagg
     8521 tatttcaatg tcggcaaccg ctggcgtgct gttgttcact tttgttgccg gtggcgtggt
     8581 ttgtgttgga acatcgctgg tcgatttatt taaccgtttt ctgttcagcg ccaaaattat
     8641 ttttctggtg gtgatgctgg tattgctgct accgcatatt cacaaagtga atcttttaac
     8701 cctgccgttg caacaggggc tggcactgtc tgcaatcccg gtgattttta cgtcgtttgg
     8761 ttttcacggt agcgtgccga gtattgtcag ctatatggat ggcaacattc gtaagctacg
     8821 ctgggtgttt ataatcggta gtgccatccc cctggtggca tatattttct ggcaggtggc
     8881 gacgcttggc agcattgatt caacaacctt tatgggattg ctggctaatc atgctggatt
     8941 aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg agctggcggt
     9001 gcatttattt gctgatttag ccctcgccac gtcatttctc ggcgttgcgt taggcttatt
     9061 tgattatctg gctgatttgt ttcagcgttc aaataccgtt ggtggacgat tgcaaactgg
     9121 tgcaattacc tttctgccgc cgttggcgtt tgcactgttt tatccacgag gatttgtgat
     9181 ggcgctgggt tacgccggtg tggcgctggc ggtactggca ttgattatcc cttcactatt
     9241 gacctggcaa agcagaaagc acaatcctca ggcgggttac cgggtcaaag gtggtcgtcc
     9301 ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc
     9361 ggcagggttg ttaccagaag tggggtgatc agatagcctc aaattcctta ttgggtgcca
     9421 gaattaacgc tgacacccaa tgtgacctct taatgcaggc agcactgctt aaatttctta
     9481 ccactgccgc acggacaagg atcgttacgc cccggtttct cttctgcttt gatcggttgc
     9541 tgaacagctt tttcctgcgg atgcgccatc cagtatgcat gtaaatcaag cgccgccagt
     9601 cgaatggcat ctacgctctc ttcaaacgct tctggcgaca ttttctctac ccgctcgaag
     9661 ttttcctcag taccgtgcag cgcaatcgcc tccagcgctg gttttaaaga atcgggcaac
     9721 gttgaccagt cagaaagtgc cacgccccgc atatagccaa agcaccactc ctcaacaatc
     9781 gtcagctcgc tgccatcaac ttctcgcaag ccgaataacg gctcaaactg ctccgggaat
     9841 tcgttcagac gctctgcggt atcggccata tgttgaaaag ccaggttcat aaagcgcgtc
     9901 atctctttct ctgacgccca acgcggcaca tagtcagccc caccccacac ggcaaccagc
     9961 cactgttctg gttcaatctc ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc
    10021 tccgccacat caaggatggc gtggtcagtg ttgtatttgg tcagaatatc gtccagccat
    10081 tccaactcac tttcgtttaa cggtcccgtt ttcatacgct tttccttgtg gatctcaact
    10141 cgccagcacc tatcttacat gccggtccgt atcagagata ctttttgagt ggctttgccg
    10201 gtgattaaaa attaaggagg gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag
    10261 gtggtttcac gacactgctt attactttga ttttattctt atctttccca tggtacccgg
    10321 agcgggactt gaacccgcac agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc
    10381 gattccacca tccgggctcg ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta
    10441 gacggatttg caatccgcta cataaccgct ttgttaacgc gccaaattct tcaggccttt
    10501 cagccagaca tccgcttgac gccgatgtct tttaaactgg agcgggaaac gagactcgaa
    10561 ctcgagacct cgaccttggc aaggtcgtgc tctaccaact gagctattcc cgcattcatc
    10621 aagcaatcag ttaatcactt gattttatta tcgtctggca atcagtgccg ccgttcgatg
    10681 cgttgcattc tacttacctg gcgcgatgag tcaacgatat ttttcaccac ttttgatcgt
    10741 ttgctgaaaa ttacgccgaa acgatcactg atcaagcaaa tctgcacgcg cagcgctcaa
    10801 atattgcaac attgaccaca gagtcagtac cgcagccaca aagaaaagtg caataccgac
    10861 gtactcaacc caaatgttcg gacgccacag cagccaggcc aacgccacca tctgggcagt
    10921 ggttttcact ttcccaatcc aggagacggc cacgctactg cgtttaccca actccgccat
    10981 ccattcgcgt agcgcagaaa taataatttc acgggcgatc atcgttgccg ccggtaatgt
    11041 cacccaccag ctgtgataat gctcggttac cagcaccatg gcgatagcca cgagaacttt
    11101 atctgccaca gggtcaagga aagcaccaaa ccgggtactc tggttccagc ggcgtgccag
    11161 aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa atgagcgcgg cggcaaacgg
    11221 cgaccaggtg acaggcagat aaaagaccaa tacaaagaat gggataagga tgacacggaa
    11281 cagtgtaagc aacgtaggga tattaaattg cataatgacg ggtaactatc tgttgtcagt
    11341 aagattaccc ctatgttgct acagagacat caatgtttca acgaccagaa gatcttttct
    11401 gccagacctt gcgaaatacc cggcactttt gcaatttcct cgacgctggc gttacgtaaa
    11461 ccttgcaaac cgcccatata tttcaacaac atttgccgac gttttggccc gacgccttca
    11521 atggtttcca gggaactggt atttttgacc ttcgcccgtt ttttacggtg cccgccaatc
    11581 gcgtgatcat gtgattcatc gcgaatatgc tggataacat gcagcgcggg tgaatctggc
    11641 ggcaaactaa atccctcacc ttccggctca aagaacagcg tttccagtcc agccttacga
    11701 tctgctcctt tggcaacgcc aagtagcagc ggatgatttt tatcccatga gacatccagt
    11761 tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc cgccgtcgat aaggatcaca
    11821 tccgggatct tactgtcgtc aatggcttta ccataacgcc gacgcagcac ctgattcatc
    11881 gccgcataat catcgcccgg cgtgatgcct gtaatgttat agcgccgata ctccgcacgc
    11941 agcgggccgt tagcatcaaa caccacacag gaagcgacgg tttgttcgcc catggtatgg
    12001 ctgatgtcaa agcactccat ccgcttcact tccggcaatt tcaacacgct ggcaagcgcg
    12061 gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc tggttaaggc cgtcgccgca
    12121 ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc gaggtttggt ttgaacatta
    12181 atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca gcgttttatc gctaagatta
    12241 aaatcgagca ggatctcacc cggtaaggtg cgcatctggc tgccttgtaa atagaactga
    12301 cctacgaagg tttctaccac ctcgctcagt tccgtaccgc caggcacttt cgggaaatag
    12361 ctgcggctgc cgagcacttt gccctgacga atgaacaata cgtggacaca agccatgccc
    12421 gcatcgaacg ccacaccaat aacgtcaagg tcgtcgccgg tattggaaac aaactgtttt
    12481 tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac atgccgcttc ttcaaactcc
    12541 agattctggc tggcagtttc catacggcta atgagttgcg taagcacctg atcatctttg
    12601 ccagacaaaa acaggcgcac atactcgacc tgctgagcgt attcttcttc actcaccagt
    12661 ccttcaacgc acggtcccag acagcgccct atctggtatt gcagacacgg acgcgagcga
    12721 ttgcgataaa cactattttc gcactggcga atggggaaaa tcttttgcag tagcgccagt
    12781 gtttcacgta cggcatagcc attcgggaac gggccgaaat attcaccttt ggcatgcttt
    12841 gcaccacgat gcatcgccag acgcgggtgg gtatcgccac tcaggaagat aaaaggataa
    12901 gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat agagtttgat gtagttgtgt
    12961 tccagcaaca gcgcttcggt ttctgtatgc gtaaccgtta catcaatttg ctggatctgg
    13021 gcgaccagcg cttcggtttt gcgcgaagcg aggttgctgc ggaaatagct ggaaagccgt
    13081 tttttcaggt ctttcgcttt gccgacatag ataaccgtac caccagcatc gtacatgcga
    13141 taaacgcctg gctggctggt tacggttttt aaaaacgctt ttgcgtcaaa ctgatcactc
    13201 actgacttga taatgtctcc gcattacaca gaccatggcg aattgccagg tgagtcagct
    13261 caacatcgcc atgaatgttt agtttactga acatacgata gcggtagctg ttcaccgttt
    13321 tcggactgag attgagctgt tctgagatct cattgacctt ctggccctta gtgatcatca
    13381 gcataatctg caattcacgt tcagacaaac tggcaaatgg gctttctgtt ttttctggtt
    13441 cgatctggct taacgccatt tgttgagcga tatcagaagc aatgtaacgc tgccctgaat
    13501 agacagaacg aatcgcactc acgacttcct gcggagccgc gcctttgctg aggtagccag
    13561 cagcacctgc ctgcatgact ttcgctggta aagggttttc tgtatggacc gtaagcatga
    13621 tgattttaac atcagctgtg gaacgcgcga ttttacgcgt cgcctcaaga ccgccaatgc
    13681 ccggcatact catgtccatt agcaccacgt caacggcatt tgtccggcac cacttaacgg
    13741 cgtcttcacc gcacgatgcc tctccgacga cttttatacc cttaatatct tccagaatgc
    13801 gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag tagaacgttg atcaaaggaa
    13861 tatctccaga aatagggata acgctactga tagttagtca ttcgtatatt agcggttttt
    13921 attgcaactt tgaaacgtta aaaatggtgg gtattcgatt tttctctcgt ttttgtcaat
    13981 tcgattgtcc acagttatgg tcacgcccgt caccaacggc ttaccggatc gtttcacagt
    14041 aagccctacg atgaaatgtg acaaaaattg actttattca gcaaaaatga aaatcagccc
    14101 gctgaatatg taacattaat taacccgaac aatacgggca aaaaacattc atgataaaaa
    14161 tatttattgg tcattatatt aacgtatttt atagcactgc cgatatcacg ctcaaaaaac
    14221 aaccactgct atttttagca aagcttatgg tatactccgc cgccttaaca tttttcacca
    14281 caaattttca ttgcaacatg acgaggaaaa taaatgagta cgcctgattt ttctactgcc
    14341 gagaataatc aagaactggc aaatgaagtc tcctgcctga aagcgatgct gacgctgatg
    14401 ctgcaggcga tgggacaagc tgacgcgggc cgcgtgatgt taaagatgga aaaacagctt
    14461 gcgctgatcg aagacgaaac ccaggctgca gtattttcca aaacggttaa gcaaattaaa
    14521 caagcctacc gtcagtaata taaaaccggc tgagagcgtg cctttcagcc ggttattgac
    14581 aaaatcatcg ctatctctgc tgatgtcttc ccttccgccg tccatttcag aatttctttt
    14641 tcgcgcttgc tgaaattcat ctctggcgtc atcactattt catcatttaa acgcatcaga
    14701 gccatcagac tttcgcgcac cagtaactgc atttttaatt gcagttcatc actaagaatg
    14761 ggtatttcgc gcgtgctgca acgggaaaag gacaaaaagc ccagcgcccg atttggcagc
    14821 attaaatact gagtgacacc gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc
    14881 tgtgcttcgc tgaataagtc atcattccac attaaatggc cctgactaaa gttttcaggg
    14941 ttcagcacag gatcaattgc gagaaaattt tttgcctgat aataactaac ccacgactca
    15001 gggtaattgg tgtagaaagc cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat
    15061 aacgaatagt aatcgtactc cagctgctga gcctgaagct ctatttcatg gtaggcctct
    15121 tctgcggcct ccatcctctg aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc
    15181 gtatcctgca tagtaaaccg caacgcccct gagagtgagt atcatttata atgataattc
    15241 aaactatcac ataaataaga tttatatata atttatatta ttcaggcaat gaattacttt
    15301 tgcaagccat cgcattctct tatgttatta atgagttatg ctgatttgtt aagcagtttt
    15361 atcacgcttg aaatggcgtc ccgccccgac aggtgaatcg tcggggctga ttttttctta
    15421 ttattgcagc agaagcttct cgaggaactg gcgggtgcga ggctgctggg gatcggcaaa
    15481 taacgctttt gctgccccct gctcgactat ccgcccctgg tccataaaga tcgcccggtc
    15541 cgcaacatcc cgggcaaagc tcatttcgtg tgtcacaatc accatcgtgc gcttttcctg
    15601 cgccagctgg cggatggtgt tcaggacttc gcccaccagc tctggatcca gcgccgacgt
    15661 tggctcgtca aacaaaatca cctcaggacg cattgccagt gcacgcgcaa tcgcaacacg
    15721 ctgctgttga ccgccagaca aacgacgtgg atagctggtt tctttaccgg ccagcccaac
    15781 tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat
    15841 caccggccct tcaataatgt tttccagcac cgtacgatgc ggaaacaaat taaagttctg
    15901 gaagacaaac ccgacgtgct gacgcaactg gcgaatcagc gatttttgct gacttaatga
    15961 acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg gttgttccag
    16021 cagatttatg ctacgtagca atgtggtttt gccggaacca ctcggaccga taattgccac
    16081 cacttcgcca ggctttacct cgaggtcgat accgtgcagc accgtctgac cgtggaattt
    16141 tttcaccagg ttcttaactt caatggcact cattttggtt ctctctcctg gcgattaagt
    16201 tgattctcaa aatggttctg caacgtcgat aacacagtcg ccatgatcca gtagatgagc
    16261 gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga
    16321 cggaacagct ccggcacctg gattgtcgcg gccagcgacg tatcttttac cagactgata
    16381 aagctgttcg acagcggcgg caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc
    16441 atggtctgcc acggcgtcat accaatactg gcagcggctt cccactgacc tttatcaata
    16501 gaagaaatgg cggcgcgcag cgtttcggcg gcataggcgg cagtgttcag cgacagacca
    16561 atcattgctg acggaatggg atctaattcg ataccaaact gcggcaggcc gtaatagatc
    16621 ataaacagtt gtgcgatgag cggcgtacca cgaaaaatag agatataaaa acgcgccagc
    16681 cagcgcacgg gccagattgg cgacaggcgc atcagcgcga gaataaaccc gagtagtaag
    16741 ccaaaaaaca tgccgccgat actgagttgc agggtatacc cagccccttt caacaggaac
    16801 ggcaaggaat caataaccag ttgtatactt tcttgcatga gcgttcccgg agctaaacgt
    16861 ggggatgata ggcgaacagc gcaggcgcgc cgccggtatg aataaacaga atcggccctt
    16921 catctttgaa gcgtttctga ctgataccgt caatcagccc cgccatcgct tttccggtat
    16981 acacaggatc aagcagaatg ccttccagac gcgccagcaa tttcactgct tccatgcctt
    17041 cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg
    17101 atgcggtcag ctccagttct ttcgcaatcg cctgttgtag gttaaccact ttcggcaatt
    17161 gatcggcaac ggaacgcgac acggtcacgc caatcagttc gctttcaggc atcaggtgtt
    17221 ccagcccaac agccagtccg gcgtgagttc cggcactgcc cgatgcgact accaccgacg
    17281 aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc acataaccta
    17341 gcgcgccaag agcattagaa ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt
    17401 cgactcgcgt cgccagttct tccagttggg cattgggatc ggtcagtgcg tcgcacattt
    17461 caatctgggt attgaacaga tccagcaaca aacgattgcc gttggttaaa tagttttctg
    17521 cggttgtgcc aataggattt tccagcagcg ccacgcagtg cagaccgagt ttcgccgcga
    17581 ctgcggcagt ctggcgcaca tggttagact ggatcgcgcc ggcagtaatc agcgtatcgg
    17641 caccttcgcg cagggcatct gccgcgagaa attccagctt acgtaattta ttgccgccca
    17701 ttgccatggg ggtgacctca tcccgtttga tgaaaatttc ccgtccaaga taatcagaaa
    17761 agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg
    17821 ttaaattatg cagtggcata acagcctccg atgtgtgttg ttgtgatttt cttattatgc
    17881 acgctgaaaa cgcgtaaata aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc
    17941 tgattatttg gtcacatcag caccaaacca tttttcggaa agagcttgca gagtgccgtc
    18001 tttttgcatt tccgcaattg catcattcac cgctttcagc aggtcttcat ttcctttacg
    18061 cagcgccacg ccagactcct gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt
    18121 ggttttcttc accagatcca gcgccgccag acgatcaacg aggatcgcat cgatacgccc
    18181 tacgcgcaga tcctgatatt tggtcgggtc atcatcatag gtacgcacat cgacaccctg
    18241 aacattctgc cgcagccact cttcatagtt ggtgcccaga ccgacaccca cttttttgcc
    18301 tttcagatca gcagccgttt taatggtgcc ttcgttacct tttttcacca gcgcctgaat
    18361 accggaaatg gtgtaagggg tagagaaatc gtattttttc ttgcgctcgt cagaaatggt
    18421 gacctgatta atcaccacat caatgcgttt agagtccagc gacgccagca taccgtccca
    18481 tttggtcggt tttagtgacg cctcaacgcc aagatgtttt gccagctgtt gggcaaattc
    18541 cacttcaaaa ccggttaatt tgccgtcatc cccctgaaaa ctgaacggcg gataagtgcc
    18601 ttccagccct accagcagcg tgccgctctc tttgacttta ttaagcagac cttcatctgc
    18661 aaaactttta acgctcatgc ccgcaaccag cgccacggcc atcacaccca tcaatgcctg
    18721 acgtcccaga tgtgctaatt tcatattcac cccgaatgtt gttatgtctg tttgcagtgt
    18781 agagccattg tttgtaaaca caaaaacaac tccgctacat cttattctta tttaatatat
    18841 atcagaagca ggcaggctgg aggatttctg cacaagtccg gtacaggttt ggcgctgata
    18901 gtgttgatac ttccgtaatg cgatgcggta attgttggtg ctggtggtgg gcagccacgg
    18961 ggcgaggatt tcatcaagga aaccgtcctg cagctgatcg agggaaatat tttgctcatg
    19021 tagatgatta cccaaacggc gcagacgaac gacatattca cgcaccgtac ctgggctcat
    19081 ttcagtttgc tcaaaaagaa atttcttaaa gccgataata tcgaaaaaat cactctgcgt
    19141 tttgcaatgt aaatcaccgc aaaatcggca caatgccgcc catgattgtt gttccgtttg
    19201 ccagcctttt tcatcaagca gcgtgtccag gcgggaaatc tcgattttat tcactatttt
    19261 gccgtcgcga actaaggtaa tgcgatcgag taatttacgg caatgcgcgc aatgggtctg
    19321 gctgtgttta aagtctttaa gatagcggct taatggccgt cttttcaggt gctgcaccat
    19381 cattaagaac tcctggtagt caaagttaaa gtgcggcatt tactgacgtt ataacttacc
    19441 cagtttagtg cgtaaccgtt taatgtcctg gctgtgtaac tgactgaccc gcgattcccc
    19501 gacctccagc accgcgccaa tctctttgag attcagctct tcctggtaat agagggttaa
    19561 taccagtttt tcgcgctccg gcaacgtttc gatggcttcc atcacccgct ggcgcagatt
    19621 actgtccagc agttgttgta gcgggttttc tcgctgatga tcatcagtaa ccagttcgat
    19681 gctatcgccg tgctcttcac gccactcatc gtaggagaag agctggctgt tattggtgtc
    19741 gagcagcatt tgacgataat cggcaatatc gatccctaaa cgttccgcta cctcagtttc
    19801 cgtggcgttg cggccaagtt cctgctccag ttgccctatt gcctgtgcca cttcacgcgc
    19861 gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga agttcatcca gcatagcgcc
    19921 acggatacgc tacactgcgt aagttgtaaa tgccgttcct tgtagggcgt catagcgttc
    19981 gacagcatta agtaacccaa tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc
    20041 gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc agcgggacat aacgctgcca
    20101 cagcgagtgt ttatccatta caccttcagc ggtatagagt gaattcacga taaacagccc
    20161 tgcgttatat gagttatcgg catgattatc cgtttctgca gggtttttaa tcggacgatt
    20221 agtgggtgaa atgaggggtt atttgggggt tacgcaaaaa gccgaatgta aaaaaccccg
    20281 ccggtggcgg ggtttgagag gcattcagac gattaaccct gcagcagaga cagaacctgc
    20341 tgcggtacct ggttggcttt tgccagcacg gagttaccgg cctgctggat aatctgcgct
    20401 ttcgacatgt tggacacttc ggtcgcatag tcggcgtcct gaatacggga ctgcgcttca
    20461 gacaggttgg tagtggtgtt gttcaggttg gtgactgcgg aatccaaacg gttttgcacc
    20521 gcacccaggg aggagcggaa tttgtcgatg gagctgatgg cttcatccag agctttcagg
    20581 ggatcggctg tcgtagctgc ttttgtttca gcatctgtcg tcaatttacc atcagcatct
    20641 ttataaactg ccttaccgct gccgttagtc actgaaccat catcctgtag gtaaaaatct
    20701 acagtatcag atgtggtcaa cgcctcgttg ttatcaacat aaaccgaatc aacacctttt
    20761 gttacagcgc cagttgtgcc gttgacgcta taacctgcag tagtgaaatc aacattgttt
    20821 cccgaggttg atacagcttt atcgaaagct gcctgatcaa cttttgcacc tgttactgaa
    20881 taagtaatag tgtccggcgt agtactatta ccggtaaagg agatagaggt accgccaatg
    20941 tcaatggttg tcgctttggt attattggct gcaagtgtgg acagactagc ctcagtcaaa
    21001 gaagcaccag agccgttttt cgttaagcgc ccagttgtat caatgtagag tttatctcca
    21061 ttgcttgccg taattttacc atcactgctg atctgaacat cctgtgcaga accatcgata
    21121 gtaatagtgc ccttagcggt atcaccaact cccggggtca aatatttctg aacatcggca
    21181 gctgaagccg ttgtggtatc aaaagagtat gacttacttg cactgtcata cttgtaattc
    21241 gttgcactag ccgccgttcc gaagccgtta ttaatggttg cctgaacagt atcaccatca
    21301 ctaactccag ccagcaaatc agacgcttta gcagcatcgt aacccgcact cacagtatat
    21361 ttgttgccga ccactgttgc attagcagct accaagtcag ctttagatgc agcagtatta
    21421 gccacagccc cgccaccatt cacattaaac ccattcagcc ccagcgtatc agagtcaatt
    21481 ttcttcagat caatagtgat agtctggccg tcattcgcac caacctgaat tttcatcgaa
    21541 ccgtcttttg ccagtacgtt cacgccgttg aactgggtct gaccggatac gcggtcaatt
    21601 tcgtcgagac gggatttgat ttcgtcctga atggagtcca gatccgaatc agagttagtc
    21661 ccggtagaag cctgaaccgt cagctcacgg atacgctgta agttgttgtt gatttcggac
    21721 agcgcgcctt cagtggtctg tgcaacagaa ataccgtcgt tggcgttacg tgcagcctga
    21781 gtcaggcctt taatgttaga agtaaaacgg ttagcaatcg cctgacccgc ggcgtcatcc
    21841 ttcgcgctgt taatacgcaa gccagaagac agacgctcga tagaactcga cagcgcagac
    21901 tggttcttgt tgatattatt ttgagtgatc agcgagaggc tgttggtatt aatgacttgt
    21961 gccatgattc gttatcctat attgcaagtc gttgattacg ttttgggttt ccacccgtcg
    22021 gctcaatcgc cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga atttttttca
    22081 caaacagcca ttttttgtta gtcgacgaaa tactcttttc tctgcccctt attcccgcta
    22141 ttaaaaaaaa caattaaacg taaactttgc gcaattcagg ccgataaccc cggtattcgt
    22201 tttacgtgtc gaaagataaa aggaaatcgc atggcaagta tttcatcgct gggagtcggg
    22261 tcaggtctgg atttaagttc aatccttgat agcctcaccg ccgcgcaaaa agcgacgcta
    22321 acccctattt caaatcagca atcgtcgttt accgctaaac ttagcgccta cggtacgctg
    22381 aaaagcgcgc tgacgacttt ccagaccgcc aatactgcat tgtctaaagc cgatcttttt
    22441 tccgccacca gcaccaccag cagcaccacc gcgttcagtg ccaccaccgc gggtaacgcc
    22501 atcgccggga aatacaccat cagcgtcacc catctggcgc aggcgcaaac cctgaccacg
    22561 cgcaccacca gagatgatac gaaaacggcg atcgccacca gcgacagcaa actcaccatt
    22621 caacaaggcg acgacaaaga tccgattacc attgatatca gcgcggctaa ctcatcgtta
    22681 agcgggatcc gtgatgccat caacaacgca aaagcgggcg taagtgcgag catcattaac
    22741 gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg acaccggcct tgataatgcg
    22801 atgacactct cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta tgacgccagt
    22861 gccagcagca acggtatgga ggtctcggtc gccgcccaga atgcgcagct gacggtcaac
    22921 aacgtcgcca tcgagaacag cagcaacact atcagcgacg cgctggaaaa catcactctg
    22981 aacctgaacg atgtcaccac gggcaaccag acgctaacca tcactcagga cacctccaaa
    23041 gtgcaaacgg cgattaaaga ctgggtgaat gcctataact cgctaataga taccttcagc
    23101 agcctgacca aatacaccgc cgtagatgcg ggagctgata gccagagttc tagcaatggc
    23161 gcactgctcg gcgactccac gctgcggacg attcagacgc agttgaagtc gatgctgagt
    23221 aataccgtca gttcttccag ctataaaacg ctggcgcaga ttggtatcac gaccgatccc
    23281 agcgatggca aactggaact ggatgccgac aaactcaccg ctgcactgaa aaaagatgcc
    23341 agcggcgtag gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac aaccaccatc
    23401 ggcagcaacc tgaccagttg gctttcgaca acgggcatta ttaaagccgc taccgatggc
    23461 gttagtaaaa ccctgaataa gttaactaaa gactacaacg ccgccagcga tcgcattgat
    23521 gcgcaggtcg cgcgctacaa agaacaattt acccaactgg acgttttaat gacctcgtta
    23581 aacagcacca gcagctactt aacgcagcag ttcgaaaaca acagtaattc caagtaatca
    23641 ttattcatcg ggagacaggt catgtacgcg gcaaaaggca cccaggccta tgcacaaatt
    23701 ggcgtcgaaa gcaccgtaat gagcgccagc caacagcagt tggtcaccat gctatttgat
    23761 ggagtgctga gcgcactggt tagagcgcga ctgtttatgc aggacaacaa tcagcaaggc
    23821 aaaggcgtct ctttgtcaaa agcgatcaac atcattgaga acggactgcg ggtgagtctt
    23881 gatgaagaga gcaaagacga attaacccaa aacttaattg ctctttatag ctatatggtc
    23941 aggcgtttgc tgcaagccaa tttacgcaac gatgtctcag cagtcgaaga ggtggaagca
    24001 ttaatgcgta atattgccga tgcctggaaa gagtctttac tctccccttc ttcgattcag
    24061 gacccagtct gatgaacaat gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa
    24121 aaagccagct catgttacgc ctggcaacgg aagaacaatg ggacgaactc atcgctagcg
    24181 aaatggcgta tgtgaatgcg gtgcaggaga tcgcacacct gactgaagag gttgccccgt
    24241 ccactacgat gcaggagcag ctccgcccga tgctgcacct gattctcgac aacgaaagca
    24301 aggtaaagca gttattacag attcggatgg atgaactggc gaaactggtc ggtcagtcat
    24361 cggtgcaaaa atcggtgtta agtgcctatg gcgatcaggg cggctttgtg ctggctccgc
    24421 aggataacct cttttgaatc ttaatgagcc gatggctcgc gaataatccg attacggcta
    24481 cgcttctaat attccccttg aatggagtcg aagaatgcgt aatcccacgc tgttacaatg
    24541 ttttcactgg tattacccgg aaggcggtaa gctctggcct gaactggccg agcgcgcaga
    24601 cggttttaat gatattggta tcaatatggt ctggttgccg cccgcctata aaggcgcatc
    24661 gggcggctat tcggtcggct acgactccta tgatttattt gatctaggtg agtttgatca
    24721 gaaaggcagc atccctacta aatatggtga taaagcacaa ctgctggccg ccattgacgc
    24781 cctgaaacgt aatgacattg cggtgctgct ggacgtggta gtcaaccaca aaatgggcgc
    24841 ggatgaaaaa gaagctattc gcgtgcagcg tgtaaatgct gatgaccgta cgcaaattga
    24901 cgaagaaatc attgagtgtg aaggctggac gcgttacacc ttccccgctc gtgccgggca
    24961 atactcgcag tttatctggg atttcaaatg ttttagcggt atcgaccata tcgaacaccc
    25021 tgatgaagat ggcattttta aaattgttaa cgactatacc ggagaaggct ggaacgatca
    25081 ggttgatgat gaactcggta atttcgatta tctgatgggt gagaatatcg attttcgcaa
    25141 tcatgccgtg acggaagaga ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg
    25201 cgacggcttt cgtcttgatg cggtcaaaca tattccggca tggttttata aagagtggat
    25261 cgaacacgta caggaagttg cgccaaagcc gctgtttatt gtggctgaat gctggtcgca
    25321 tgaagtcgat aaactgcaaa cgtatattga tcaggtggaa ggcaaaacca tgctgtttga
    25381 tgcgccgctg cagatgaaat tccatgaagc atcgcgcatg ggtcgcgact acgacatgac
    25441 gcagatcttc actgggacgc tggtggaagc cgatcctttt cacgccgtaa ccctcgttgc
    25501 caaccacgac acccaaccgt tgcaagccct cgaagcaccg gtcgaaccgt ggtttaaacc
    25561 gctggcgtat gccttaattt tgttgcggga aaatggcgtt ccttcggtgt tctatccgga
    25621 cctctacggt gcacattacg aagatgtcgg tggtgacggg caaacctatc cgatagatat
    25681 gccaataatc gaacagcttg atgagttaat tctcgcccgt cagcgtttcg cccacggtgt
    25741 acagacatta tttttcgacc atccgaactg cattgccttt agccgcagtg gcaccgacga
    25801 atatcccggc tgcgtggtgg tcatgtcgaa cggagatgat ggcgaaaaaa ccattcatct
    25861 gggagagaat tacggcaata aaacctggcg tgattttctg gggaaccggc aagagagcgt
    25921 agtgacagac gaaaacggcg aagcgacctt cttttgcaac ggcggcagcg tcagcgtgtg
    25981 ggttatcgaa gaggtgattt aaatccatcc ccggcggcaa gccggggaga tttcattacg
    26041 gcagtggcgt cggtaacggc gctttatcca gcgcggcggc acactctgcc gtcgggcgat
    26101 ccacacgctc catcgtcata ccatcgtact caatggtgtt accgtcacgc tcaacctcgt
    26161 acagctcacg ttttacggta acgttagtga gatcatcaga catcaatgtc aacttacccg
    26221 gcacggcaat cccccgctgc cactgacggc aatccaaggt atcgccctct ttagtgacaa
    26281 tcaggctgcc tatcgcttcc ggactgacca gcgcacgttg cggccctttg gtctgccagt
    26341 agcccgccag ccagtccggc gcgggggttt tcacgacatt gttatagttt tcgacttcgg
    26401 cacagcccgc cagcaacatg agcgcgcctg caattgcgag ttttttcatc attcgtcctg
    26461 catgagaaga aaagaggatt gtggcattaa agctctgatg tcgccagcct ttccaggttc
    26521 agagagaaat ttgatctaac tattattttt gtgaattatt accccgaaaa taaatttgca
    26581 tggtgtatga ttcgcggctc ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc
    26641 atggcagcaa ttcaaacacg cctggttgat taaattctgg gcacccatcc ctgcggtcat
    26701 cgcggcgggt attctctcca cttactattt tggcattact ggcacctttt gggctgtcac
    26761 gggtgaattt acccgttggg gcggtcagct cctgcagctg tttggcgtcc atgctgaaga
    26821 gtggggttat tttaaaatta tccatctgga aggatcgcca ttaacccgga tcgacggcat
    26881 gatgatcctc ggtatgtttg gcggctgctt tgctgctgcg ctgtgggcca acaatgtcaa
    26941 actacgcatg ccacgcagcc gtatccgcat tatgcaggcc atcattggcg gcattatcgc
    27001 cggttttggc gcgcgtctgg caatgggctg taacctggcg gcgttcttta ccggcattcc
    27061 tcagttctcg ctgcatgcct ggttctttgc catcgccact gccattggtt catggtttgg
    27121 cgcgcgcttt accctgctgc ccatcttccg tattcccgtg aaaatgcaga aagtttctgc
    27181 cgcctcaccg ctgacgcaaa aaccggatca ggcgcggcgt cgttttcgtc tcgggatgct
    27241 ggtctttttc ggcatgctgg gctgggcgct gctcacggcg atgaaccaac caaaactggg
    27301 gctggcaatg ctgtttggcg tcggctttgg tttactgatt gaacgtgcgc aaatctgctt
    27361 tacttcggcg ttccgcgata tgtggatcac cggacgtacc catatggcga aagcaatcat
    27421 tatcggtatg gcggtgagtg ccatcgggat cttcagttac gtacagttag gcgttgaacc
    27481 taaaatcatg tgggcgggac caaatgcggt aattggtggt ttactttttg gttttggcat
    27541 cgtgctggca ggcggctgcg aaaccggctg gatgtaccgc gcggtagaag gccaggtgca
    27601 ctactggtgg gtcggtctgg gcaacgtgat cggctcaacg attctggcgt attactggga
    27661 tgatttcgct ccggcgctgg ccaccgactg ggacaaaatc aacctgctga aaacttttgg
    27721 tccgatggga ggcctgctgg tgacatattt gctgctgttt gctgcgctga tgttgattat
    27781 cggctgggaa aaacgcttct tccgccgtga ggcaccgcag actgctaagg agatcgcatg
    27841 aaaaatatcg ttcctgatta ccgccttgat atggtgggtg aaccctgccc ttatccggca
    27901 gtcgcaaccc ttgaggcgat gccgcaatta aaaaaaggtg agatcctgga agtggtgagc
    27961 gactgtccgc agtcgatcaa taatattcca ctggatgcgc gtaatcacgg ctacacggtg
    28021 ctggatattc agcaagacgg gccgaccatt cgttatttaa ttcagaagta atccctcatt
    28081 cccgctgtgg cccacagcgg gaacatcgtt acctctgaca catagatttt acccgccccc
    28141 tctcctattc ttttcagtat gatctcagga ggttgctatg tgtggacgct ttgcccaatc
    28201 ccaaacgcgt gaggattacc ttgcgcttct cgcggaagat attgaacgcg atattcccta
    28261 cgatcccgaa cccattggca gatacaatgt cgcgccggga accaaagttc ttctgctcag
    28321 tgaacgtgat gaacaccttc atctggatcc ggttttctgg ggatatgctc ccggatggtg
    28381 ggataaaccg ccgctgatta acgcccgcgt agaaactgcc tcaaccagtc gcatgtttaa
    28441 accgctctgg caacatggtc gggcaatctg ttttgccgat ggctggtttg agtggaaaaa
    28501 agaaggcgac aaaaaaagcc ttattttatc tatcgcgctg acggacaacc tgtttttatg
    28561 gccgcgatag gtagcatacc gtttgagcgt ggtgacgaag ccgaaggatt tttgatcgtc
    28621 actgcggcgg cagatcaagg tctggtagat attcatgacc gccgcccgct ggttctgtcg
    28681 ccagaagctg cacgggaatg gatgcggcaa gacattggtg gtaaagaagc ctcagaaatc
    28741 gctaccagaa gctgtgtgcc agcaaaccag ttcacctggc accctgtgtc gcgcacggtg
    28801 ggtaatgtca aaaaccaggg cgcggagtta attcaacctg tttgttgaca tcctccacgc
    28861 cctgaatgac gaggacccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc
    28921 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    28981 cgctaaaatg ttacgagctc catttacatc ggcgttcgct gtatatccac atacctggca
    29041 tctgaattta ctttgtgaca ggcgattttc tttcgctgta tgaccacagc acgcgcaacg
    29101 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccacgccaga gctgcttgta
    29161 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    29221 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    29281 gctgaccttc aaatcctcaa tgactatcat t
//
LOCUS       DAJTYH010000047        29226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTYH010000047 DAJTYH010000000
VERSION     DAJTYH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            367..2139
                     /gene="aspS"
                     /locus_tag="OO609_002028"
     CDS             367..2139
                     /gene="aspS"
                     /locus_tag="OO609_002028"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6633447.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2257..2709
                     /gene="nudB"
                     /locus_tag="OO609_002029"
     CDS             2257..2709
                     /gene="nudB"
                     /locus_tag="OO609_002029"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6633448.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2738..3478
                     /locus_tag="OO609_002030"
     CDS             2738..3478
                     /locus_tag="OO609_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6633449.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3513..4034
                     /gene="ruvC"
                     /locus_tag="OO609_002031"
     CDS             3513..4034
                     /gene="ruvC"
                     /locus_tag="OO609_002031"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6633450.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4036..4638)
                     /locus_tag="OO609_002032"
     CDS             complement(4036..4638)
                     /locus_tag="OO609_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6633451.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4708..4773
                     /gene="yobI"
                     /locus_tag="OO609_002033"
     CDS             4708..4773
                     /gene="yobI"
                     /locus_tag="OO609_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6633452.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            4912..5523
                     /gene="ruvA"
                     /locus_tag="OO609_002034"
     CDS             4912..5523
                     /gene="ruvA"
                     /locus_tag="OO609_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6633453.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5532..6542
                     /gene="ruvB"
                     /locus_tag="OO609_002035"
     CDS             5532..6542
                     /gene="ruvB"
                     /locus_tag="OO609_002035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6633454.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6689..7474)
                     /gene="znuB"
                     /locus_tag="OO609_002036"
     CDS             complement(6689..7474)
                     /gene="znuB"
                     /locus_tag="OO609_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6633455.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7471..8226)
                     /gene="znuC"
                     /locus_tag="OO609_002037"
     CDS             complement(7471..8226)
                     /gene="znuC"
                     /locus_tag="OO609_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6633456.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8305..9237
                     /gene="znuA"
                     /locus_tag="OO609_002038"
     CDS             8305..9237
                     /gene="znuA"
                     /locus_tag="OO609_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6633457.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9253..10575
                     /gene="mepM"
                     /locus_tag="OO609_002039"
     CDS             9253..10575
                     /gene="mepM"
                     /locus_tag="OO609_002039"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6633458.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10695..11666
                     /gene="lpxM"
                     /locus_tag="OO609_002040"
                     /gene_synonym="msbB"
     CDS             10695..11666
                     /gene="lpxM"
                     /locus_tag="OO609_002040"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6633459.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11797..13239)
                     /gene="pyk"
                     /locus_tag="OO609_002041"
     CDS             complement(11797..13239)
                     /gene="pyk"
                     /locus_tag="OO609_002041"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6633460.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13367..14236)
                     /gene="hexR"
                     /locus_tag="OO609_002042"
                     /gene_synonym="yebK"
     CDS             complement(13367..14236)
                     /gene="hexR"
                     /locus_tag="OO609_002042"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6633461.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14573..16048
                     /gene="zwf"
                     /locus_tag="OO609_002043"
     CDS             14573..16048
                     /gene="zwf"
                     /locus_tag="OO609_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6633462.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16283..18094
                     /gene="edd"
                     /locus_tag="OO609_002044"
     CDS             16283..18094
                     /gene="edd"
                     /locus_tag="OO609_002044"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6633463.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18131..18772
                     /gene="kdgA"
                     /locus_tag="OO609_002045"
                     /gene_synonym="eda"
     CDS             18131..18772
                     /gene="kdgA"
                     /locus_tag="OO609_002045"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6633464.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18828..20006)
                     /gene="purT"
                     /locus_tag="OO609_002046"
     CDS             complement(18828..20006)
                     /gene="purT"
                     /locus_tag="OO609_002046"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6633465.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRPHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20140..20430
                     /gene="yebG"
                     /locus_tag="OO609_002047"
                     /pseudo
     CDS             20140..20430
                     /gene="yebG"
                     /locus_tag="OO609_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20497..20853
                     /locus_tag="OO609_002048"
     CDS             20497..20853
                     /locus_tag="OO609_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6633466.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21180..21839
                     /locus_tag="OO609_002049"
     CDS             21180..21839
                     /locus_tag="OO609_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6633467.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22049..24109
                     /gene="ptrB"
                     /locus_tag="OO609_002050"
     CDS             22049..24109
                     /gene="ptrB"
                     /locus_tag="OO609_002050"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6633468.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24106..24768)
                     /gene="exoX"
                     /locus_tag="OO609_002051"
     CDS             complement(24106..24768)
                     /gene="exoX"
                     /locus_tag="OO609_002051"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6633469.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24792..25448)
                     /locus_tag="OO609_002052"
                     /pseudo
     CDS             complement(24792..25448)
                     /locus_tag="OO609_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25550..25780)
                     /gene="holE"
                     /locus_tag="OO609_002053"
     CDS             complement(25550..25780)
                     /gene="holE"
                     /locus_tag="OO609_002053"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6633470.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            25919..26293
                     /gene="yobA"
                     /locus_tag="OO609_002054"
     CDS             25919..26293
                     /gene="yobA"
                     /locus_tag="OO609_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6633471.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26297..27169
                     /gene="copD"
                     /locus_tag="OO609_002055"
                     /pseudo
     CDS             26297..27169
                     /gene="copD"
                     /locus_tag="OO609_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27182..27523
                     /locus_tag="OO609_002056"
     CDS             27182..27523
                     /locus_tag="OO609_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6633472.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(27916..28993)
                     /locus_tag="OO609_002057"
                     /pseudo
     CDS             complement(27916..28993)
                     /locus_tag="OO609_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(28959..>29226)
                     /locus_tag="OO609_002058"
     CDS             complement(28959..>29226)
                     /locus_tag="OO609_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6633473.1"
                     /translation="LKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSYRVVPE
                     AFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7372 a         7170 c         7512 g         7172 t
ORIGIN      
        1 gccttcttgt aaatcgatca ccaccaacgc agtggtttta gcattaagtt caagcatgct
       61 gactcctgaa aaaacaaaat cgtgcctcac accttaatgc gattagccga tggcggaacg
      121 acaaattttg ttaattttta tgagttcggg caacaccttt atagcaaact ggcgtagagt
      181 ctgatttcag gcccgatata aagtggtaac gaggcgttcc agcgcgggcc aggctcttac
      241 ggtgcggcag aatttccagt ataatagccg ccttttttca ttcagttgtg acatacagct
      301 aacgctgcga cttggtcacc tgcgcagcag ggggcatccg ccggcagcta agttaaggga
      361 tatctcatgc gtacagaata ttgtggacag ctccgtttgt cccacgtggg gcagcaggtg
      421 actctgtgtg gttgggtcaa ccgtcgtcgt gatcttggta gcctgatctt catcgatatg
      481 cgcgaccgcg aaggtatcgt gcaggtattt ttcgatccgg atcgtgcgga cgcgttaaag
      541 ctggcctctg aactgcgtaa tgagttctgc attcaggtta cgggcaccgt acgtgcgcgt
      601 gacgaaaaaa atattaaccg cgatatggcg accggcgaaa ttgaagtgct ggcgtcctcg
      661 ctgactatca tcaaccgcgc agatgttctg ccgcttgact ctaaccacgt caacaccgaa
      721 gaagcgcgtc tgaaataccg ctacctcgac ctgcgtcgcc cggaaatggc gcaacgcctg
      781 aaaacccgcg ctaaaatcac cagcctggtg cgccgtttta tggatgacca cggcttcctc
      841 gacatcgaaa ctccgatgct gaccaaagcc acgccggaag gcgcgcgtga ctacctggtg
      901 ccttctcgtg tgcacaaagg taaattctac gcactgccgc aatccccaca gttgttcaaa
      961 cagctgctga tgatgtccgg ttttgaccgt tactatcaga tcgttaaatg cttccgtgac
     1021 gaagacctgc gcgctgatcg tcagcctgaa tttactcaga tcgatgtgga aacttctttc
     1081 atgaccgcgc cgcaagtgcg tgaagtgatg gaagcgctgg tgcgtcattt gtggctggaa
     1141 gtgaagggcg tggatctggg cgatttcccg gtaatgacct ttgcggaagc agaacgccgt
     1201 tatggttctg ataaaccgga tctgcgtaac ccgatggaac tgaccgacgt tgccgatctg
     1261 ctgaaatctg ttgagtttgc agtatttgcg gggccagcga acgatccgaa aggccgtgtt
     1321 gccgctctac gtgttccggg tggcgcatcg ctgacccgta agcagatcga cgaatacggt
     1381 aacttcgtta aaatctacgg cgcgaaaggt ctggcttaca tcaaagttaa cgaacgcgcg
     1441 aaaggtctgg aaggtatcaa cagcccggta gcgaagttcc ttaatgcaga aatcatcgaa
     1501 gccatcctgg atcgtactgc cgcgcaagat ggcgatatga ttttcttcgg tgccgacaac
     1561 aagaaaattg ttgccgacgg gatgggcgca ctgctcctga aagtgggtaa agaccttggt
     1621 ctgaccgacg aaagcaaatg ggcaccgctg tgggttatcg acttcccgat gtttgaagac
     1681 gacggtgaag gcggcctgac ggcaatgcac catccgttca cctcaccgaa agatatgact
     1741 gctgcagaac tgaaagctgc accggaaaat gcggtggcga acgcttacga tatggtcatc
     1801 aatggttacg aagtgggcgg tggttcagta cgtatccata atggtgatat gcagcagacg
     1861 gtgtttggta ttctgggtat caacgaagag gaacagcgcg agaaattcgg cttcctgctc
     1921 gacgccctga aatacggtac tccgccgcac gcaggtctgg cattcggtct tgaccgtctg
     1981 accatgctgc tgactggcac cgacaatatc cgtgacgtta tcgccttccc gaaaaccacg
     2041 gcggcagcgt gtctgatgac tgaagcaccg agctttgcta acccgactgc actggctgag
     2101 ctgagcattc aggttgtgaa gaaggctgag aataactgat atgactcaaa tacacgaaat
     2161 cattcgcgtt gcatcaaggc ggcaactgag tgaactccca tgagcataga taactatgtg
     2221 aatgggatga gcgaaggcag tcaacgaaga ggcagcgtga aggataaagt gtataagcgt
     2281 cccgtttcga tcttagtggt catctatgca caagatacga aacgggtgct gatgttgcag
     2341 cggcgtgacg atcccgattt ctggcagtcg gtaaccggca gcgtggaaga gggtgaaacc
     2401 gcgccgcaag ctgccatgcg cgaagtaaag gaagaggtta ccattgatgt tgtcgctgaa
     2461 caactgacct taattgactg tcagcgcacg gtagagtttg aaattttttc acatttacgt
     2521 catcgctatg cgccgggcgt gacgcgtaat acggaatcat ggttctgtct tgcgcttccg
     2581 cacgagcggc agatcgtttt cactgaacat ctggcttaca agtggcttga tgcgcctgct
     2641 gcggcggcgc tcactaagtc ctggagcaac cggcaggcga ttgaacagtt tgtaattaac
     2701 gctgcctgaa caggcagggc tatctggaga tatttttatg gcaggtcata gtaaatgggc
     2761 caacaccaga catcgtaaag ctgcgcagga tgctaagcgc ggtaaaatct tcactaaaat
     2821 cattcgtgag ctggtaaccg cggctaagct gggcggtggc gatccggacg ctaacccgcg
     2881 tctgcgtgcg gcggtggata aagcactgtc taacaacatg acccgtgaca cactgaaccg
     2941 cgcaattgca cgtggtgtgg gcggtgatga tgatgcaaac atggaaacca tcatctacga
     3001 aggttacggt cctggcggca cggcaatcat gattgaatgt ctgtctgaca accgcaaccg
     3061 taccgttgct gaagtgcgtc acgcattcag caaatgtggc ggtaacctcg gcactgacgg
     3121 ttccgtagct tatctgttca gcaaaaaagg cgtgatctcc ttcgagaaag gcgacgaaga
     3181 caccatcatg gaagcagctc tggaagcagg tgctgaagac gttgtgacat atgatgacgg
     3241 cgcgattgat gtctacactg catgggaaga aatgggtaaa gtgcgcgacg ctctggaagc
     3301 ggcaggtctg aaagcagaca gcgcggaagt ttccatgatc ccgtccacca aagctgatat
     3361 ggatgcagaa accgcgccga aactgatgcg tctgatcgat atgctggaag attgcgacga
     3421 cgtgcaggaa gtttaccata acggtgaaat ctctgatgag gttgcagcga ctctctgatg
     3481 atgcctgcta aacagcaaaa cggagacgcg tgatggctat tattctcggc attgacccgg
     3541 gttcgcgcgt gaccggctac ggcgtcatcc gccaggtagg taggcaactg tcctacctgg
     3601 gtagcggatg catacgcacc aaagtggatg atttaccgtc tcgtctgaag ctcatctatg
     3661 cgggcgtgac ggaaatcatc acccagttcc agcctgatta tttcgccatt gaacaagtct
     3721 ttatggcgaa gaacgctgac tcagccctga aactgggcca ggcgcgcggc gtggcgattg
     3781 ttgcggcggt gaatcaggaa ttgccggtat ttgaatacgc ggcacgtcag gtaaagcaaa
     3841 cggtggtagg tatgggtagt gccgaaaaaa gccaggtgca gcatatggtc cgcaccttgc
     3901 tgaaactgcc cgctaatcca caggcggatg ccgccgatgc gctggcgatt gccatcaccc
     3961 actgccacgt tagtcagaat gcgatgcaga tgagcgaatc gcggctgaac ctgacgagag
     4021 ggcgactgcg ttaagttata ccgcctcggt cagttccggc tgaggcgttt caactccctc
     4081 cgcgtgaatc aacaccaaac ccggatgatg ccacaaactg gctggcgtga cggttttacg
     4141 ctcccacgga atagaaccta agaaccagaa tttccagaaa gtgagttttg cattcggatt
     4201 gctatttaac agttgtccaa acgtctctat ttcacccacc ggaatacata gcgcctcttt
     4261 ataaatatca aagacaaatt ttgaacagaa ctggcgcgaa gattcgtatt taaaacccgt
     4321 gtggtaaagt ttgcgtagcc gggagggaac ctgttcaaca attcgttgtt tttgttgttc
     4381 tgtcagccca gcgtctaatc gctttatagc atagcgttga ttagacgaac gtttaataaa
     4441 acgagatagc gtggtgatgg ttgaaagggg aacccggctt tctgcaacca ggaagtcttc
     4501 accgttatga ccgataatga tcccgacgtg attactccag caatttgatg cagctgatat
     4561 ttgaccaaat aaggcagcac ctatacatgt aaagacgata tctccaattt catattcagc
     4621 agggtaatta atattcacgt tgtcattcca ttgaaataga tacaaatata cgtaatagca
     4681 taaaaaaatt atttttcagg agtcgcaatg tatattttta ttacgcattt cttcactgaa
     4741 tatgtaatat tcaaatattt gcttccaata taacctgttg aataaattat actgtgccat
     4801 ttttcagatc atcgggacac ctcgcaagtt ttcttcatcc ttcgctggat atctatccag
     4861 cattttttta tcatacagca ttatctttga ttcattacgc aggagcgtca tgtgataggc
     4921 agactcagag gcatcatcat tgaaaaacaa cccccgctgg tgttaattga agtgggcggc
     4981 gtaggctatg aagtgcatat gccgatgacc tgtttttatg aactccctga agcgggtcag
     5041 gaagcgatcg ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct gtacggtttt
     5101 aacaataaac aagaacgcac gttgttcaaa gagttgatca aaaccaacgg cgtcggcccg
     5161 aagttggccc tggcgatcct ctccggaatg tcagcgcagc agttcgttaa tgccgttgag
     5221 cgtgaagaag tgggggcact ggtgaaactg ccgggtattg gcaaaaaaac cgccgaacgc
     5281 ttgattgttg aaatgaaaga ccgatttaaa ggtttgcatg gcgatctctt tacgccagcc
     5341 gccgacctgg tactcacgtc tcctgctagc ccggcgaccg acgatgctga acaagaagca
     5401 gttgccgcgc tggtggcgct aggctataaa ccacaagaag caagccgcat ggtgagcaaa
     5461 atcgctcgcc ctgacaccag cagtgaaaca ttaattcgcg aagccctacg cgccgcgtta
     5521 tgaggtaaag gatgattgaa gcagaccgtc tgatttctgc cggtaccact ttgccggaag
     5581 atgtagcaga tcgcgccatt cgccctaaat tactggaaga gtatgttggt cagccgcagg
     5641 ttcgttcaca gatggagatt ttcatcaaag cagcgaaact gcgcggcgat gccctcgatc
     5701 atttgttgat ttttggtcct ccggggttgg gtaaaactac gcttgccaac attgtcgcca
     5761 atgaaatggg cgttaattta cgcacgactt ctggtccggt gctggaaaag gcgggcgatt
     5821 tggctgcgat gctcactaac cttgaaccgc atgacgtgct gtttattgat gagatccacc
     5881 gtttatcgcc agttgttgaa gaagtgctgt acccggcaat ggaagactac caactggata
     5941 tcatgattgg tgaaggtccg gcggcacgct ccattaaaat tgatttgccg ccgtttaccc
     6001 tgattggcgc aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgac cgttttggta
     6061 ttgtgcaacg tctggagttt tatcaggtgc cggatctgca atatatcgtc agtcgcagtg
     6121 cacgctttat ggggcttgag atgagtgatg acggtgcgca ggaagtagct cgtcgcgctc
     6181 gcggtacgcc acgtattgcc aaccgtctgc tgcgtcgagt gcgtgatttc gccgaagtga
     6241 agcacgatgg caccatctcg gcagatatcg ctgctcaggc gctggatatg ttgaatgtcg
     6301 atgctgaagg tttcgattat atggaccgca aattgttgct ggcggtaatc gataagttct
     6361 ttggtgggcc ggtaggtctg gataacctgg cggcagccat tggcgaagaa cgtgaaacca
     6421 ttgaagatgt actggaacct tatttgattc agcaaggctt tttgcagcgt acaccgcgcg
     6481 ggcgtatggc gacgacgcgg gcgtggaatc actttggcat aacgccgcca gaaatgccgt
     6541 aagtcggatt ggtcgctcgc gccgcatccg acactggcag tgtgtctgat gcgacactgt
     6601 tcgcgtctta tcaggccagc cagagatagc gaaccgtagg tcggataagg cgctcgcgcc
     6661 gcatccgaca aatgtgttca gcgatagatt agctggcctg ctttttcatc atactgataa
     6721 taaataacag tgccgcacat agcaccaccg acggacccgc cggcgtatcg taaaacgcgg
     6781 aaaaggttaa accgccagtt actgccacca tccccaccaa aacagcgaca ccagccatct
     6841 gttccggcgt acgggcaaag cgacgcgcag tagctgcagg aataatcagc aacgaagtaa
     6901 taatcaacgc accgacaaat ttcatcgcta caccaatcgt caatgccgtc accagcatca
     6961 acaacaattt cacttgctgt aatttcacac catcaacaaa cgccagatcc gggctaatcg
     7021 tcatcgacag caaattgcgc cattgccaga acaaaatagc caccacgatg accacgccaa
     7081 tcgcaataga gatgagatct tctggcgtca ctgccagtaa atcaccgaac aggtaagcca
     7141 tcaaatcaac acgaatgtta gacatcagac taacgaccac taggcccagc gacagggcac
     7201 tgtgcgccat aatccctaat aacgtgtcga tcgccagctg tggacgcttc tccagccata
     7261 ccagaccgcc cgccagcagc agcgtaacgg caatcaccgc atagaatgga ttcacgtcca
     7321 gcaacaaacc aaacgcgaca ccaagtaatg aggcatgagc cagcgtatca ccgaaataag
     7381 acatacgacg ccagactaca aacgaaccca gcggacccgc ggcacaggcg agcatgatcc
     7441 cggctaacca accgggaaat aataattcaa tcatgagcga tcatttcccc gacgcaaaac
     7501 aattcgtccc tgtaaatcgt gacgatgatt atgatgatgg cgatagatac ccagttgttc
     7561 agcaccacga ggaccaaaca ttgaaataaa ctccggatgc agggaaacaa cttccggtgt
     7621 gccggaacaa caaatgtggt gattcaggca cagcacttca tcggtttttg ccatcaccag
     7681 atgcagatcg tgagaaacca ttaaaacgcc acaatccagt tcacgacgca actggtcaat
     7741 aaggtcatat aacgccactt ggccatttac atccacgcct tgagtgggtt catccagcac
     7801 taataattgc gggcaattta acaatgctcg cgctaatagt acacgctgcg tttcgccacc
     7861 agagagcttt tgcatcggtg cgttaatcag gtgcccggcc tggacacgtt tcagtgcagg
     7921 caaaatatct tctttatgtg taccagggcg taagcgtaaa aaacggttta cggtcagtgg
     7981 caacgtggtg tcgagataca gcttctgcgg tacatagccg atgcgcagtt ttccgttgcg
     8041 cttgataacc ccttcatcgg gtgttaccag cccgagcact acccgtacca gtgtcgactt
     8101 acctgcgcca tttggcccaa gtaaagtcaa aatttttcca ggtttaagtt ccagcgacac
     8161 atcagagagg acgcggcgtt ggccaaaaga aaccgagaca ttttccaggg aaaccagact
     8221 tgtcatgtta attttagtct tgcagtagtc atgaaatgtt ataatatcac acttctcata
     8281 ttcattacga tgattggtcg cattatgtta cataaaaaaa cgcttctttt cgcagcatta
     8341 tccgccgctc tctggggagg tgcaacacag gccgcagatg ccgccgttgt cgcttcgctt
     8401 aaacccgttg ggttcatcgc ttctgccatt gctgatggtg taacagaaac ggaggtttta
     8461 ctgcctgacg gcgcttcaga acatgattat tcactgcgtc catcggatgt aaaacgctta
     8521 cagaacgcgg acttagtcgt ttgggttggc ccggagatgg aagcgtttat gcaaaaaccg
     8581 gtaagcaaat taccagaagc gaagcaggta acgattgcgc agcttgaaga tgtgaaaccg
     8641 ctactgatga aaagtattca cggcgatgat gatgatcacg accacgcgga aaaaagtgac
     8701 gaagatcacc atcacggcga tttcaacatg catctttggc tttccccaga gatagcgcgg
     8761 gctacagcgg ttgcaatcca tggaaaatta gtggaactta tgccgcaaag tcgagccaaa
     8821 cttgacgcca acctgaagga ttttgaggca caattggcct caaccgaaac gcaggttggt
     8881 aacgagctcg cgccgctcaa ggggaaaggt tatttcgttt ttcacgatgc ttacggctat
     8941 ttcgaaaaac agttcggact gacaccgctt ggtcatttta ccgttaaccc tgagattcaa
     9001 cctggcgcgc agcgtttaca tgaaataaga acacagttgg ttgagcaaaa agcaacctgc
     9061 gtttttgctg agccacagtt caggccagcg gtcgttgaga gcgtcgcacg agggacatcc
     9121 gttcgtatgg gaacgttgga tcccctgggg acgaatatca aactgggtaa aacaagctat
     9181 tcagaattcc tgagtcaatt agccaaccag tatgcgagct gcctgaaagg agattaatga
     9241 ggaagtgatt acgtgcaaca gatagcccgc tctgtcgccc tggcgtttaa taatttaccg
     9301 cgaccacacc gcgttatgtt ggggtcgctc accgttctta ctctggccgt cgctgtctgg
     9361 cggccctatg tttatcaccg tgatgccacg ccaattgtca aaaccattga gctggaacag
     9421 aacgaaattc gttcgctctt acctgaagcc agtgagccga ttgatcaagc tgcacaagaa
     9481 gatgaagcca ttccccagga cgaactggat gacaaaatcg ccggtgaagc gggcgtgcat
     9541 gaatatgttg tttccactgg cgatacgcta agcagcattc tcaatcagta tggtattgat
     9601 atgggtgata tcacccaact tgctgctgcc gacaaagaat tgcgtaacct gaaaatcggt
     9661 caacaactct cctggacatt aaccgcggac ggcgaactgc agcgcctcac ctgggaagtg
     9721 tctcgtcgtg aaacccgaac ctatgaccgt actgccgcta acggttttaa aatgaccagc
     9781 gaaatgcagc aaggagagtg ggttaacaat ctgctgaaag gtaccgtcgg agggagcttt
     9841 gttgccagcg ccagaaacgc cggtttaacc agcgccgaag tgagcgcagt gattaaagcc
     9901 atgcagtggc aaatggattt ccgcaaactg aaaaaaggcg atgaatttgc ggtattaatg
     9961 tctcgagaaa tgcttgatgg taaacgtgag caaagccagc tgctgggcgt acgtttgcgt
    10021 tcagaaggta aagattatta cgcaatccgc gctgaggatg gcaaattcta cgatcgtaac
    10081 ggtactggtc tggcgaaagg attcttgcga ttcccgacgg cgaaacagtt ccgtatctcg
    10141 tctaacttta acccgcgtcg aactaatccg gtgacaggtc gcgttgcacc acacagaggt
    10201 gttgatttcg ccatgccaca aggtacgcca gtgctttcag tgggtgacgg tgaagtggtg
    10261 gttgccaaac gcagtggcgc agcaggttat tatgtggcta ttcgtcatgg tcgcagctac
    10321 accacgcgtt atatgcactt gcgcaagatt ctggtgaaac cgggacagaa ggtgaaacgt
    10381 ggcgaccgta tcgcgctttc cggtaatacc ggacgttcaa ctgggccgca tctgcactat
    10441 gaagtatgga taaaccagca ggccgtaaac ccgctgacgg caaaactgcc gcgtaccgaa
    10501 gggctgaccg gctccgatcg tcgcgaattc ctggcgcagg ccaaagagat tgtgccgcag
    10561 ctacggtttg attaattaac atccattcgc agccggtacg cagtcagtac cggctttttt
    10621 tatttggtgc ggggcaagtt gtgccgctac actatcacca gattgatttt tgccttatcc
    10681 gaaactggaa aagcatggaa acgaaaaaaa ataatagcga atacattcct gagtttgata
    10741 aatcctttcg ccacccgcgc tactggggag catggctggg tgtagcagcg atggcgggta
    10801 tcgctttaac gccgccaaag ttccgtgatc ccattctggc acggctggga cgttttgccg
    10861 gacgactggg aaaaagctca cgccgtcgtg cgttaatcaa tctgtcgctc tgctttccag
    10921 aacgtagtga agctgaacgc gaagcgattg ttgatgagat gtttgccacc gcgccgcaag
    10981 cgatggcaat gatggctgag ttggcaatac gcgggccgga gaaaattcag ccgcgcgttg
    11041 gctggcaagg gctggagatc atcgaagaga tgcggcgtaa taacgagaaa gttatctttc
    11101 tggtgccgca cggttgggcc gtcgatattc ctgccatgct gatggcctcg caagggcaga
    11161 aaatggcagc gatgttccat aatcagggca acccggtttt tgattatgtc tggaacacgg
    11221 tgcgtcgtcg ctttggcggt cgtctgcatg cgagaaatga cggtattaaa ccattcatcc
    11281 agtcggtacg tcaggggtac tggggatatt atttacccga tcaggatcat ggcccagagc
    11341 acagcgaatt tgtggatttc tttgccacct ataaagcgac gttgcccgcg attggtcgtt
    11401 tgatgaaagt atgccgtgcg cgcgttgtac cgctgtttcc gatttatgat ggcaagacgc
    11461 atcgtctgac gattcaggtg cgcccaccga tggatgatct gttagaggcg gatgatcata
    11521 cgattgagcg gcggatgaat gaagaagtcg agatttttgt tggtccgcga ccagaacaat
    11581 acacctggat actaaaattg ctgaaaactc gcaaaccggg cgaaatccag ccgtataagc
    11641 gcaaagatct ttatcccatc aaataaaaaa gcctctcgcg aggagaggcc ttcgcctgat
    11701 gataagttca agtttgcttc agaatattcg aaatctgttg aactatcatt gaactatagg
    11761 ccggatgtgg cgttttcgcc gcatccggca acgtacttac tctaccgtta aaatacgcgt
    11821 ggtattagta gaacccacgg tactcatcac gtcgccttgg gtgacaatca ccaggtcacc
    11881 agacatcaag taacctttat cgcgcagcag attaaccgct tcgctggcag ctgctacgcc
    11941 gtcattagcg ctatcaaagg gcaccggcgt aacgccacga tagagagcag tcaggttcag
    12001 cgtacgttca tggcgcgaca tggcgaaaat tggtagacca gagctgatac gggaggtcat
    12061 cagcgcggta cgacccgatt cggtcatggt aatgatcgcc gtaacgccct tcaggtggtt
    12121 agctgcgtac attgccgaca tggcaatagc ttcttccaca ttgtcgaact gaacgtccag
    12181 acggtgttta gaaacgttga tgctcgggat tttttccgca cccaggcaaa cgcgcgccat
    12241 ggctgcaaca gtttctgacg gatactgccc agcggcagtt tctgcagaca gcatcacagc
    12301 gtcagtacca tccagaacgg cgtttgctac gtccatgact tctgcacgcg tcggcatcgg
    12361 gttagtaatc attgactcca tcatctgggt cgccgtgatt accgctcggt ttagctgacg
    12421 cgcacgacgg atcaacgctt tctgaatgcc gaccagttcc gggtcgccaa tttccacacc
    12481 gaggtcgcca cgtgcaacca ttaccacgtc agaggcgagg atgatgtcat ccattgcatc
    12541 ctggctgcaa acggcttccg cacgttcaac cttggcaaca attttcgcat cacatcctgc
    12601 atcgcgtgcc aggcgacggg catagttcag atcttcgcca cagcgtggga aggagacagc
    12661 caggtaatct acgccaatca acgccgcagt cttaatgtct gctttgtctt tttcggtcag
    12721 cgcttcagcc gacaaaccgc cgccaagttt gttgatacct ttattgttgg agaggggacc
    12781 accgacggtg acttcggtga acactttcat gccctgaact tccagtactt ttaactggac
    12841 gcgaccatcg tccagcagca ggatgtcacc aggcacgacg tcagtaggca ggcctttgta
    12901 gtcgataccg actttttctt tgtcgccttc acctttaccc aggttggcgt cgagcaggaa
    12961 tttatcccca atattgagga aaactttgcc ttctttaaag gtggatacac ggattttggg
    13021 cccctggagg tcacccagaa tagccacatg acgccccagt tttgcggcaa tctcacgaac
    13081 tttatccgcg cgcattttgt gatcttcagg cgagccgtga gaaaagttca tacgtacaac
    13141 gttggcaccc gccgcgataa ctttttcaag attattatcg cgatctgttg ctgggcctaa
    13201 cgtggtaacg atttttgttc tgcgaagcct tttggacatg taatactccg ttgactgaaa
    13261 caaccaggtg ttgcttgaac atgaaatccg aatgaaataa cgccgcgatg gcgataaatt
    13321 aatctcattc ggatgtcgta taagattagg acagtgacag tcgcttttag cgatcgtcac
    13381 ttaaattaag taactgctta tcaaaacgcg attccttcag cgcttccttg acccgcttca
    13441 agttatctct gaattttgca ccgcgtcgca aagtaaatcc tgtcgccagc acatctatca
    13501 cggtcagctg tgcaagtcga gaaaccatgg gcatataaat gtcagtatct tccggtacgt
    13561 cgagggtaat tgccagcgtt gcttcccggg cgagcggggt acccgcagag gtgagggcaa
    13621 tcaccatggc gtcgttttcg cgtgccagct gcgccagctc gaccagattt tttgttcttc
    13681 ctgtgtgaga aatcagcacc accacgtctc cgtcgctaca attcatacaa ctcatgcgtt
    13741 gcagcacgat atcatcggag tacaccaccg gaacattaaa acgaaagaac ttattcatcg
    13801 catcgtgggc aacggcggca gaagagccta atccgaaaaa ggcgattttt tttgcctgag
    13861 tgagcaagtc gacggcgcgg ttgatggcag atttatccag tgaatgacgg acatgatcga
    13921 gcgttgccat tgcggactca aatattttcc ccgtgtatga ttcaacgctg tcatcttcat
    13981 tgacgttgcg attaacatag ggagtgccat tcgccagact ttgtgccaga tgaagtttaa
    14041 aatcaggaaa accgcgcgtg tccatgctgc gacagaaacg attcaccgtc ggttcgctaa
    14101 cattggcttc cagcgccaga gcagcaatac tcgaatggat cgcgttatcg ggcgaagcca
    14161 gaatgacctc ggcaactttg cgctctgatt tgctcaaatg ttccagctga gactggattt
    14221 tttccagcat attcatgatg taaagagact cacgggtaat gacgatttcc gcactgaaag
    14281 aaatcgaaat gcagttttgt cagatattac gcctgtgtgc cgtgttaatg acaaaagcag
    14341 ataaaaaagt tgttattttt ttcataacat gatcagtgtc agatttttac cgaatggaaa
    14401 acgatgattt ttttatcagt tttgccgcgc tttgcgcgct tttcccgtaa tcgcacgggt
    14461 ggataagcgt ttacagtttt cgcaagctcg taaaagcagt acagtgcacc gtaagaaaat
    14521 tacaagtata ccctggctta agtaccgggt tagttaactt aaggagaatg acatggcggt
    14581 aacgcaaaca gcccaggcct gtgacctggt cattttcggc gcgaaaggcg accttgcgcg
    14641 tcgtaaattg ctgccttccc tgtatcaact ggaaaaagtc ggtcagctca acccggacac
    14701 ccggattatc ggcgtagggc gtgctgactg ggataaagcg gcatatacca aagttgtccg
    14761 cgaggcgctc gaaactttca tgaaagaaac cattgatgaa ggtttatggg acaccctgag
    14821 tgcacgtctg gatttttgta atctcgatgt caatgacact gctgcattca accgtctcgg
    14881 cgcgatgctg gatcaaaaaa atcgtatcac tattaactac tttgccatgc cgcccagcac
    14941 ttttggcgca atttgcaaag ggcttggcga ggcaaaactg aatgctaaac cggcacgcgt
    15001 agtcatggag aaaccgctgg ggacgtcgct ggcgacctcg caggaaatca atgatcaggt
    15061 tggcgaatac ttcgaggagt gccaggttta ccgtatcgac cactatcttg gtaaagaaac
    15121 ggtgctgaac ctgttggcgc tgcgttttgc taactccctg tttgtgaata actgggacaa
    15181 tcgcaccatt gatcatgttg agattaccgt ggcagaagaa gtggggatcg aagggcgctg
    15241 gggctatttt gataaagccg gtcagatgcg tgacatgatt cagaaccacc tgctacaaat
    15301 tctctgcatg attgcgatgt ctccgccatc tgacctgagc gcagacagca tccgcgatga
    15361 aaaagtgaaa gtactgaagt ccctgcgccg catcgaccgc tccaacgtac gcgaaaaaac
    15421 cgtacgcggg caatatactg cgggcttcgc ccagggcaaa aaagtgccgg gatatctgga
    15481 agaagagggc gcgaacaaaa gcagcaatac agaaaccttc gtggcgatcc gcgtcgacat
    15541 tgataactgg cgctgggccg gtgtgccatt ctacctgcgt actggtaaac gtctgccgac
    15601 caaatgttct gaagtcgtgg tctatttcaa aacacctgaa ctgaatctgt ttaaagagtc
    15661 gtggcaggat ctgccgcaga ataaactgac tatccgtctg caacctgatg aaggcgtgga
    15721 tatccaggta ctgaataaag ttcctggcct tgaccacaaa cataacctgc aaatcaccaa
    15781 gctggatctg agctattcag aaacctttaa tcagacgcat ctggcggatg cctatgaacg
    15841 tttgctgctg gaaaccatgc gtggtattca ggcactgttt gtacgtcgcg acgaagtgga
    15901 agaagcctgg aaatgggtag actccattac tgaggcgtgg gcgatggaca atgatgcgcc
    15961 gaaaccgtat caggccggaa cctggggacc cgttgcctcg gtggcgatga ttacccgtga
    16021 tggtcgttcc tggaatgagt ttgagtaata tcagcgctta tcctttatgg ttattttacc
    16081 ggtaacatga tcttgcacag attgtagaac aatttttaca ctttcaggcc tcgtgcggat
    16141 tcacccacga ggcttttttt attacactga ctgaaacgtt tttgccctat gagctccggt
    16201 tacaggcgtt tcactcataa atcctctgaa tgaaacgcgt tgtgaatcat cctgctctga
    16261 caactcaatt tcaggagcct ttatgaatcc acaattgtta cgcgtaacaa atcgaatcat
    16321 tgaacgttcg cgcgagactc gctctgctta tctcgcccgg atagaacaag cgaaaacgtc
    16381 gaccgttcat cgttcgcagt tggcatgcgg taacctggca cacggtttcg ctgcctgcca
    16441 gccagaaaac aaagcctctt tgaaaagcat gttgcgtaac aatatcgcca tcatcacctc
    16501 ctataacgac atgctctccg cgcaccagcc ttatgaacac tatccagaaa tcattcgtaa
    16561 agccctgcat gaagcgaatg cggttggtca ggttgcgggc ggtgttccgg cgatgtgtga
    16621 tggtgtcacc caggggcagg atggaatgga attgtcgctg ctaagccgcg aagtgatagc
    16681 gatgtctgcg gcggtggggc tgtcccataa catgtttgat ggtgctctgt tcctcggtgt
    16741 gtgcgacaag attgtcccgg gtctgacgat ggcagccctg tcgtttggtc atttgcctgc
    16801 ggtgtttgtg ccgtctggac cgatggcaag cggtttgcca aataaagaaa aagtgcgtat
    16861 tcgccagctt tatgccgaag gtaaagtgga ccgcatggcc ttactggagt cagaagccgc
    16921 gtcttaccat gcgccgggaa catgtacttt ctacggtact gccaacacca accagatggt
    16981 ggtggaattt atggggatgc agttgccagg ctcttctttt gttcatccgg attctccgct
    17041 gcgcgatgct ttgaccgctg ccgctgcgcg tcaggttaca cgcatgaccg gtaatggtaa
    17101 tgaatggatg ccgatcggta agatgatcga tgagaaagtg gtggtgaacg gtatcgttgc
    17161 actgctggcg accggtggtt ccactaacca caccatgcac ctggtggcga tggcgcgcgc
    17221 ggccggtatt cagattaact gggatgactt ctctgacctt tctgatgttg taccgctgat
    17281 ggcacgtctc tacccgaacg gtccggccga tattaaccac ttccaggcgg caggtggcgt
    17341 accggttctg gtgcgtgaac tgctcaaagc aggcctgctg catgaagatg tcaatacggt
    17401 ggcaggcttt ggtctgtctc gttataccct tgaaccatgg ctgaataatg gtgaactgga
    17461 ctggcgggaa ggggcggaaa aatcactcga cagcaatgtg atcgcttcct tcgaacaacc
    17521 tttctctcat catggtggga caaaagtgtt aagcggtaac ctgggccgtg cggttatgaa
    17581 aacctctgcc gtgccggttg agaaccaggt gattgaagcg ccagcggttg tttttgaaag
    17641 ccagcatgac gttatgccgg cctttgaagc gggtttgctg gaccgcgatt gtgtcgttgt
    17701 tgtccgtcat caggggccaa aagcgaacgg aatgccagaa ttacataaac tcatgccgcc
    17761 acttggtgta ttattggacc ggtgtttcaa aattgcgtta gttaccgatg gacgactctc
    17821 cggcgcttca ggtaaagtgc cgtcagctat ccacgtaaca ccagaagcct acgatggcgg
    17881 gctgctggca aaagtgcgcg acggggacat cattcgtgtg aatggacaga caggcgaact
    17941 gacactgctg gtagacgaag cggaactggc tgctcgcgaa ccgcacattc ctgacctgag
    18001 cgcgtcacgc gtgggaacag gacgtgaatt attcagcgcc ttgcgtgaaa aactgtccgg
    18061 tgccgaacag ggcgcaacct gtatcacttt ttaagacgac aaatttgtaa tcaggcgaga
    18121 gaaaactctg atgaaaaact ggaaaacaag tgcagaatca atcctgacca ccggcccggt
    18181 tgtaccggtt atcgtggtaa aaaaactgga acacgcggtg ccgatggcaa aagcgttggt
    18241 tgctggtggg gtgcgcgttc tggaagtgac tctgcgtacc gagtgtgcag ttgacgctat
    18301 ccgtgctatc gccaaagaag tgcctgaagc gattgtgggt gccggtacgg tgctgaatcc
    18361 acagcagctg gcagaagtca ctgaagcggg tgcacagttc gcaattagcc cgggtctgac
    18421 cgagccgctg ctgaaagctg ctaccgaagg gactattcct ctgattccgg ggatcagcac
    18481 tgtttccgaa ctgatgctgg gtatggacta cggtttgaaa gagttcaaat tcttcccggc
    18541 tgaagctaac ggcggcgtga aagccctgca ggcgatcgcg ggtccgttct cccaggtccg
    18601 tttctgcccg acgggtggta tttctccggc taactaccgt gactacctgg cactgaaaag
    18661 cgtgctgtgc atcggtggtt cctggctggt tccggcagat gcgctggaag cgggcgatta
    18721 cgaccgcatt actaagctgg cgcgtgaagc tgtagaaggc gctaagctgt aaaagtcaaa
    18781 atgcccgatc taggatcggg catttttgta gcgttttttt gccgggttta accctgtact
    18841 tttacctgtc cggcggcgtg cttcgcgcgt tcaatggcgt caacaacact ctctgaagta
    18901 gccagtacca cccccagatg acggctgcca tcaatttccg gcttaccaaa taaacgaatc
    18961 tgcaaatctg cgcctacggc attctgcaca ttatcaaacg tgacattctg actggtcagt
    19021 tgtggcagaa taacggcaga agctgcagga ccatactgac ggatcccgcc aacaggaagt
    19081 ccgaggaagg cacgtacatg cagggcaaac tctgagagat cttgagaaat taacgtcacc
    19141 atcccggtat catgtggacg aggggagacc tcactgaaaa tcacctcatc accacagaca
    19201 aacagctcga caccaaacag accataaccg cccagtgcca gcaccacttt acgggcaatc
    19261 tcctgcgcac gttcaagggc aagcgggctc atttgctgtg gttgccagga ttcacggtag
    19321 tcgccatctt cctggcgatg acctactggt gcacagaaat ggacgccatc caccgcgctg
    19381 acggttagca gggtaatttc gaagtcaaac ttaacgacgc cttcaacaat tacgcggccc
    19441 gctccggcgc gaccgccttg ctgagcgtat tcccatgcct gagcaagttg ctctgcagag
    19501 cgaataaacg tctgcccctt gccggaagag ctcatcaccg gttttacaat gcagggatag
    19561 ccaatggcgg caaccgcctc gcggaaaagg cttttgctat cggcaaaacg ataagtggaa
    19621 gtgggcagct gcagctcttc tgccgccagg cgacggatac cctcgcgatt catcgttaat
    19681 ttcgttgcgc gagcgcaggg gacaacattc agtccttcct cttcaagttg gatcagcata
    19741 tcggtggcaa tagcttcgat ctccggcacg atataatgtg gtttttccag ttcaaccaca
    19801 cggcgtaatg catcaccatc aagcatatta atgacatggg ggcgatgcgc gacatgcatg
    19861 gctggtgcgt cggcatagcg atcgacggca atcacctcta cgccgagacg ctgacactcg
    19921 attgccactt ctttacccag ttcaccggag cctaataaca tcacgcgagt tgctgccgga
    19981 cgcagcgcag tgcctaataa cgtcataacg aatatcccct gattaattcc gcgcgcagta
    20041 taaacgaaaa cgtttgcgtg tgtctttatt gctgatgttg atttctcaac cgaaaagaaa
    20101 tatactgtat aaaatctcag ttattatgag aggctgcaaa tggcggttga agttaaatac
    20161 gtagtcattc gtgagggcga agagaaaatg tcgtttacca gcaaaaagga agccgatgct
    20221 tatgacaaaa tgcttgatac ggcggatctg cttgacacct agctgacaaa ttctccagtg
    20281 caaatggaag acgagcaacg tgaagccctt tcgctatggc tggcagaaca aaaagaggtg
    20341 ctgagcacca ttctgaaaac cggcaaatta ccgtccccac aggtcgtcgg tgctgaatcc
    20401 gaagaggaag atgcctcaca cgcggcctga agcaacttgc gctcctgaca atttgtacca
    20461 tgctttctcc tgaatcattg tgctatggag aaaaacatga aaaaaagagg ggcgttttta
    20521 gggcggttgt tggtttctgc ctgcgcatca gttttcgctg ccaataatga aaccagcaag
    20581 tcggtcactt tcccaaagtg tgaaggtctg gatgctgccg gaattgccgc gagcgtaaaa
    20641 cgtgattatc aacaaaatcg cgtggcgcgc tgggcagatg atcaaaaaat tgtcggtcag
    20701 gccgatcccg tggcctgggt cagtttgcag gacattcagg gtaaagatga taaatggtca
    20761 gtaccgctaa ccgtgcgtgg taaaagtgcc gatattcatt accaggtctg cgtggactgc
    20821 aaagcgggaa tggcggaata tcagcggcgt taatggcttc tgataattcg attgaccgtt
    20881 ggcggatgcg gcgcgagtgc cttgtccgtc ccagaacgca ccacaatcgt aggcctgaga
    20941 agacgcaaca gcgtcgcatc gggcaatcag catcaaacaa tgccactcaa aacgacaaac
    21001 tactttcacc taaaataccc ttatcacctg atatacagta aataccttac ctggcgcgaa
    21061 tttctttgaa taacggccta ccgccccgct tatttgcgca tcttttgtct cgatatacat
    21121 tttctgacat cctcatccgc tatgctacag gcactaaaaa tggttattga ggagcaaata
    21181 tggctaactg gttaaatcaa ctgcaatccc tgcttgggca aagcagttct tctacctcct
    21241 cgtctgcgga tcagggattg ggcaaactgt tagtgtcagg cgcattaggc ggactggctg
    21301 ggctgctggt cgcaaataaa tcagcacgta aacttcttac gaaatatggc accaacgcgt
    21361 tactggttgg cggcggagcg gtagcgggta cggtgctgtg gaataaatac aaagataaaa
    21421 ttcgcgcggc gcatcaggac gaaccgcagt ttggcgcgca aagtacgccg ctggatgagc
    21481 gtacggaacg tttgatcctt gcgctggtct ttgccgctaa aagtgatggt catattgatg
    21541 ccaatgaacg tgcggcaatc gaccagcaat tgcgtgaagc cggcgtggaa gagcaggggc
    21601 gtgtactcat tgagcaggca atcgaacaac cgctggatcc acaacgcctg gctaccgggg
    21661 tccgcaatga agaagaggcg ctggaaatct atttcctgag ctgcgcggct attgatattg
    21721 accattttat ggaacgcagt tacctgaatg cactgggtga cgcgctgaaa atcccccagg
    21781 atgttcgtga aggcattgaa cgcgatctcg aacagcaaaa acgcactttg gcggaataat
    21841 tccgctctct ggatagcctg aagttgacta tttcgggcta tccagaaccg cacagccatt
    21901 tgaacaggct gattttctct gtctgcataa cattcgatat ccgcgccatt acctgtcatc
    21961 atctaagcaa tgacgaccct gtttcgcttg catccccggt gagttttgcc acccttataa
    22021 gatgtttcca accagaaaga acaataacat gctaccaaaa gccgcccgca ttccccacgc
    22081 catgacgctt catggcgata cgcgcatcga taattactac tggctgcggg acgatacgcg
    22141 ttctcagcca aaagtcctgg actacctgca acaagaaaat agttacggtc atcgggtgat
    22201 ggcctcacaa caagccttgc aggatcgcat cttaaaggaa atcatcgacc gcattccgca
    22261 aagagaagtt tctgcgccct acataaaaaa tggctaccgc tatcggcata tttatgaacc
    22321 aggctgtgaa tatgctatct accagcgtca atcggcattc agcgaagagt gggatgagtg
    22381 ggaaacattg ctcgatgcca acaagcgcgc agctcatagt gagttttatt cgatgggcgg
    22441 aatggcgatt acgcccgata acaccattat ggcgctggca gaagattttc tttcccgacg
    22501 ccagtacggc attcgttttc gtaatctgga aacaggtaac tggtacccgg aactgctgga
    22561 taacgttgaa cccagctttg tctgggcaaa tgactcctgg actttctact atgttcgcaa
    22621 gcatccggtg acgctgctgc cttatcaggt ctggcgtcac gccatcggta cgccagcatc
    22681 gcaagataca ctgatctacg aagaaaaaga cgatacctat tatgtcagcc tgcataaaac
    22741 gacgtcgaag cactatgtcg tcattcatct ggccagcgcc accaccagtg aagttcgcct
    22801 gctggacgcg gaaatggccg atgccgagcc gtttgttttt ctgccgcgcc gcaaagatca
    22861 cgaatacagc cttgatcact accagcatcg tttttatctg cgttccaatc gccacggcaa
    22921 aaactttggc ttataccgta cccgtatgcg tgatgagcaa cagtgggaag agttaattcc
    22981 gccacgcgaa aacatcatgc tggaagggtt tacgctgttt accgactggc tggtggttga
    23041 agagcgtcag cgcgggttaa ccagtttgcg ccaaattaac cgcaagaccc gggaagtcat
    23101 tggcattgcc tttgatgatc cggcctatgt gacctggatt gcctacaatc cagaacctga
    23161 aaccgcgcga ttgcgttatg gttattcttc catgaccaca ccagacactt tgtttgaact
    23221 ggatatggat accggtgagc gtcgtgtatt aaaacaaaca gaagtgcctg gttttgatgc
    23281 ggcgaattac cgcagtgaac acctgtggat agtcgcccgt gatggcgtcg aagttccggt
    23341 ttcgctggtc tatcatcgca aacattttcg caaaggacac aacccgttgc tggtgtatgg
    23401 ctatggttct tacggcgcaa gtattgatgc cgatttcagt tttagccgct tgagtttgtt
    23461 agatcgtggc tttgtctacg ccattgtcca tgttcgcggc ggtggtgagc tggggcaaca
    23521 atggtacgaa gacggtaaat ttctgaagaa gaaaaatacg tttaatgatt atcttgatgc
    23581 ctgcgatgca ttgttaaaac tgggctatgg ctctccttcg ctctgttatg cgatgggcgg
    23641 aagtgcgggg ggcatgttga tgggcgttgc aattaatcaa cgcccggaat tattccacgg
    23701 cgttatcgcc caggtaccgt ttgttgatgt tgtaacaacg atgcttgatg aatcaattcc
    23761 tcttaccact ggtgagtttg aagagtgggg aaatccgcag gatccgcaat attacgagta
    23821 catgaaaagc tacagcccgt atgacaacgt caccgcacag gcttatccgc atttactggt
    23881 aacgaccggt ttacacgatt ctcaggtgca atattgggaa ccggcaaaat gggtcgctaa
    23941 attgcgcgag ctgaaaaccg atgaccatct tttattgctc tgtaccgaca tggactcagg
    24001 ccatggcggt aaatctggcc gctttaaatc gtacgaaggc gtagcgatgg aatatgcttt
    24061 tctggtcgcg ctggcgcagg gaacattacc cgctacgcct gcggattaag tattttccag
    24121 ataatgtttc agtgttaaac gcagctccgg gctcatgctg tccaggttat taaataacca
    24181 gcgcagatag cccggatagc gttcggcaac gtcggaaacc gctttgccac ggtatttgcc
    24241 aaaggtgaag gttgtcatca acgacggacg tccggtgata tcggccatct gttctgccgt
    24301 ccagccggag gtgttcataa tatcgataag caacgcggcg gtgatataac agtcatacaa
    24361 cgcgcggtga tgatgcaggc ccagcggtgt ctgtacattg agcttgcgtg ttttatataa
    24421 cgccatattg ctgtacttga gccctggcca caaacgacgg gccagtttca tagtgcaaat
    24481 ccactcaccg ggcatctcag gcagtacgcg gcggtcaaag ctggcgttgt gcgcgacata
    24541 ccattcacta ccgtaatagt gtgggatcac atcttcaatc cacggtttat cggcgaccat
    24601 ggcttcggtg atgcgatgaa tcgccatcgc ttgtggacta ataggacgat cggggcgcac
    24661 caggaggctc atggggttga cgatttttcc gtcaatgaca tcaacagagg caatctcaac
    24721 gatccctccc tgcaaaccgc aggtttctgt atcgataatg cgcaacatgg catgatccag
    24781 gccgaaaaag cctagcgtaa tggaatgata tcgccttgcc aaccctgtga tgaacgctga
    24841 ccgactaaca ataatgaacc acgatcggcg cgaacgatga gacgaccata ttcatcccat
    24901 agtgcactgt taccacgggc atttgccatc agcacggcaa tcgagtactg atgtgaaaaa
    24961 aattgtaagc gacgggccga cgccagcagg tcgggttctc ccagacattg ccctgtcgta
    25021 aacagtgaac aggtcgggtc catatccata ccttgcggtt gttcatcgac caccgtgatg
    25081 gttctggaac gacggcccag acatgcacca tgactctgat ggtagttccc aggcgttttt
    25141 cgccacggtg cgaacaccgc aattccacga ataaagcgat cgttatattc aacgggaagg
    25201 ccggcaatga tggtcattcg ctaggtcgtt gcggcataac aaagcgggtc taacagtgaa
    25261 agatcgggtg gggcaggaag ggcacgtcgc gaataatcac accccagtaa agaaagcgat
    25321 ggaaaaacta acagctgaca ttgttgcctg gcggcggccc tcacaaactc cagatgatga
    25381 gctacctgtt cggtgagtga ggttttacga ggctcatatt gcgctgctgc aactttccag
    25441 aacgacataa tgacttcctt ttcaaaatga ctgcaaatta tgggggcggt ttagaaaata
    25501 ctcataattt agcaggcgtt atgtaagaaa gtgtaactct gtataagttt tatttaagtt
    25561 tgggctcgta aggtaaacgt gacagattga ccgaagccaa acggtgggca ataagccgct
    25621 cgcgaaacca gctgcgcaaa tgttctggct gttcacgttc aaccgcttca gcgatcaccg
    25681 gcatattgta gcgttcttta aaagccaccc cggccgccgc caaatcgaca ttcactttat
    25741 ccatttctgt ttgatccagt ttagccagat tcttcagcat tcttaatctc ctcatcattc
    25801 gcgggcaaaa gttactaaga gtgagcctgg atggcaagcc ggaattgatg atcctcgact
    25861 cagacattag acaaggttat gctctgtaaa aaagttatta caaaaaggaa tagataatat
    25921 ggcttcaact gcacgctccc ttcgctacgc gctggcaatc ctgaccactt cgttggtgac
    25981 accttccgtc tgggcacatg cgcatttaac gcatcagtat cctgcggcaa acgcgcaagt
    26041 gacagctgcc ccgcaggcaa tcactttaaa cttctcggaa ggcgttgaaa caggattcag
    26101 tggtgcaaaa atcacggggc caaaaaacga aaatattaaa acattaccag cgaagcggaa
    26161 tgagcaggac caaaagcaac tgattgttcc actggccgat tcgctgaaac ccggaaccta
    26221 caccgttgac tggcatgttg tttcggtgga cgggcataaa acgaaaggac actacacctt
    26281 tagcgtgaaa taaatcatgc tggcgtttac ctggatcgcg ctgcgattta tccatttcac
    26341 ctcgttgatg ctggtctttg gctttgcaat gtatggtgcc tggctggccc ccttaatgat
    26401 tcgtcgcttg ctgacgaagc gttctctgcg tttacaacag cacgctgccg tctggagttt
    26461 gatcagtgcc actgcaatgc tcgccgttca gggcgggttg atgggaacgg gatggacaga
    26521 tgtattttcg ccaaacattt ggcaggctgt tttgcaaacg cagtttggtg gcgtctggtt
    26581 atggcaaatt gttctcgccc tcgtaacgtt gatcgtcgcc cttatgcaac cgcggaatat
    26641 gccacgtttg ctgtttatgc tcactaccgc gcaatttatt ctgctggcgg gagtggggta
    26701 tgcgacgctg aatgaagggg taacagcgaa aatccatcag actaatcacg cgattcacct
    26761 gatttgtgcg gcggcgtggt ttgggggatt actgccggtg ctctggtgta tgcaactcat
    26821 caaaggccgt tggcgacact aggctattca ggcgctgatg cgtttttcct ggtgcgggca
    26881 ttttgcggtg atcggcgtac tggcaagcgg cgtgcttaat gcattgttga ttaccggatt
    26941 tccccccacg cttacgactt actggggcca actcttattg ctcaaagcca tcctggtgat
    27001 gattatggtg gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca tgaggcagga
    27061 tgaagatcgt gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg cgataggtgc
    27121 ggtagtgctg gtgatcatca gcctgcttgc gactctcgaa cctttttgat ggattacgaa
    27181 aatgatgaaa aaaagtattc tggcgtttct gttactcacc agttctgcag cggcgctggc
    27241 ggctccacag gtgataaccg tgagtcgttt tgaagtgggt aaagacaaat gggcgtttaa
    27301 tcgcgaagag gtaatgctga cttgccgacc gggtaatgct ttgtatgtca tcaacccaag
    27361 taccctcgtg cagtatcctt taaacgatat cgcacaaaag gaagttgcca gtgggaagac
    27421 caatgcccaa cccatttcgg tgattcagat tgatgatcct aacaatcccg gcgaaaaaat
    27481 gagtctggca ccgtttatag aacgagctga aaaactctgt taattaccta aaatagcctt
    27541 ttgatttcca ataaaaaaac cgcctcagtt ctttcaccag aacgggcggt ttttaacatt
    27601 taagctgatg agcaccgcgc tttttattga ccattttgca cgcaaactgg aaaacctggc
    27661 gtcgtcatct attcttaaag ggcaaggcaa ctaagcctgc attaatgcca acttttagcg
    27721 cacggctctc tcccaagagc catttccctg gaccgaatac aggaatcgta ttcggtcttt
    27781 ttttaattgt atttaaaatc aattagttgc aaacgtctcc ccgaaattcc ccgaaattta
    27841 ctcgaatttc tgtattccgg tcttttttgg ttatatcaca accaaaatac atttaacaat
    27901 ccatttacgt taaaatcaga gcagtaagta cgttttttct ctctcatcaa gatacatttt
    27961 tgttgtcttc tccgatgtgt ggccaagtag acgctgagca aattcttctc cacatatttc
    28021 tttgtacaat cgtccagcca ggcttctgat ttcgtgaaaa gttggtgggt tttcactgaa
    28081 cagtgatacc tgttaatttt ctggctgcga caaatttctt tgtcaggccg tccgggtgaa
    28141 tgctgccgtc agggctgttt tttctaatcc cggcactgat tagataatcc cccctactta
    28201 ccatgcggca ctgttcaact actgcaccaa gtcgtaaacc agcgacagga aggctgagtg
    28261 acagggggat agcaatcatc attcctgtct taatttgcct gatgtggaga cgatcatcat
    28321 aaatatcact gaaccgcata ttcgttatgt cttcgcgacg ttgtcctgtt acaagggcta
    28381 agtccatagc taatgggaac catgccggaa gttgatctgc tgcctccctg atgcagttgt
    28441 atgtctttag tttcagccgt tctcttgtaa ctactatttt cggtgctctt gttggcgtta
    28501 ctggattttg agatatacgt ccttcaacaa tggcttcgcg gaacatatca gataacacag
    28561 aacgcattga tcctgccatc gtgtttttcc ctccttcaat ctacaaatca agaaactcgg
    28621 cgatatggcg agtggttatt tctgtcagta aaacccctcc taatttttct tttattgtct
    28681 ccagttgatt tacccgagtt ttataagtat ttctggacgc ttttctcctt ataagaatcg
    28741 ttttgtaacg ttcaatccag cctgccatag taaatgagtc gaccccttta agcttttcaa
    28801 ttagggcagc aggagagtag tttttgtata tataatgatt tgcttcaatt gcctgcgcta
    28861 ctgcatctct tcttgaaatt ttacctagtg taaattcttc tttcgtcaga gggttgcgcc
    28921 agtaatatgc tttgtctctc cttcgatatg ttaagtttct aggcaaattg ggatcgtatt
    28981 ttttccgctg catgttttaa cttctccatt aacggactgt ctctcccttg tcgcccatta
    29041 ggctgatggt gtgttatatc ggtatcaacc ttatttgggt tgatatagaa agcctccgga
    29101 accaccctgt aactcctccc gtgtagttca ggtgcaggat aaatgtttcc attccttgcc
    29161 catcgtctca gcgttgatat tgatggtggg ttatccggat atctgagttt tccccacgtt
    29221 ttgagt
//
LOCUS       DAJTYH010000048        28917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTYH010000048 DAJTYH010000000
VERSION     DAJTYH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1012
                     /gene="mdtN"
                     /locus_tag="OO609_002059"
     CDS             <1..1012
                     /gene="mdtN"
                     /locus_tag="OO609_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6633474.1"
                     /translation="APRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDV
                     VPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSV
                     DAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEAD
                     LNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGRIISLKTS
                     VGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDS
                     IGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLE
                     PQ"
     gene            1012..3054
                     /gene="mdtO"
                     /locus_tag="OO609_002060"
     CDS             1012..3054
                     /gene="mdtO"
                     /locus_tag="OO609_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6633475.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPDTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3051..4517
                     /gene="mdtP"
                     /locus_tag="OO609_002061"
     CDS             3051..4517
                     /gene="mdtP"
                     /locus_tag="OO609_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6633476.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4715..4954
                     /locus_tag="OO609_002062"
     CDS             4715..4954
                     /locus_tag="OO609_002062"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633477.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            4964..5134
                     /locus_tag="OO609_002063"
     CDS             4964..5134
                     /locus_tag="OO609_002063"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6633478.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5183..6595
                     /gene="fdhF"
                     /locus_tag="OO609_002064"
     CDS             5183..6595
                     /gene="fdhF"
                     /locus_tag="OO609_002064"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6633479.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6596..6862
                     /locus_tag="OO609_002065"
     CDS             6596..6862
                     /locus_tag="OO609_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6633480.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            6956..7645
                     /locus_tag="OO609_002066"
     CDS             6956..7645
                     /locus_tag="OO609_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6633481.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7723..9036)
                     /gene="gltP"
                     /locus_tag="OO609_002067"
     CDS             complement(7723..9036)
                     /gene="gltP"
                     /locus_tag="OO609_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6633482.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9378..9974)
                     /gene="nrfG"
                     /locus_tag="OO609_002068"
     CDS             complement(9378..9974)
                     /gene="nrfG"
                     /locus_tag="OO609_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6633483.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(9971..10354)
                     /gene="nrfF"
                     /locus_tag="OO609_002069"
     CDS             complement(9971..10354)
                     /gene="nrfF"
                     /locus_tag="OO609_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6633484.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10347..12065)
                     /locus_tag="OO609_002070"
     CDS             complement(10347..12065)
                     /locus_tag="OO609_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6633485.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12085..13041)
                     /gene="nrfD"
                     /locus_tag="OO609_002071"
     CDS             complement(12085..13041)
                     /gene="nrfD"
                     /locus_tag="OO609_002071"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6633486.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13038..13709)
                     /gene="nrfC"
                     /locus_tag="OO609_002072"
     CDS             complement(13038..13709)
                     /gene="nrfC"
                     /locus_tag="OO609_002072"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6633487.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13706..14272)
                     /gene="nrfB"
                     /locus_tag="OO609_002073"
     CDS             complement(13706..14272)
                     /gene="nrfB"
                     /locus_tag="OO609_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6633488.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14317..15753)
                     /gene="nrfA"
                     /locus_tag="OO609_002074"
     CDS             complement(14317..15753)
                     /gene="nrfA"
                     /locus_tag="OO609_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6633489.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16146..18104
                     /gene="acs"
                     /locus_tag="OO609_002075"
                     /pseudo
     CDS             16146..18104
                     /gene="acs"
                     /locus_tag="OO609_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18304..18618
                     /locus_tag="OO609_002076"
     CDS             18304..18618
                     /locus_tag="OO609_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6633490.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18615..20264
                     /gene="actP"
                     /locus_tag="OO609_002077"
     CDS             18615..20264
                     /gene="actP"
                     /locus_tag="OO609_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6633491.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20442..21734
                     /locus_tag="OO609_002078"
     CDS             20442..21734
                     /locus_tag="OO609_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6633492.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(21888..23537)
                     /locus_tag="OO609_002079"
     CDS             complement(21888..23537)
                     /locus_tag="OO609_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6633493.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23688..25037)
                     /gene="ghxP"
                     /locus_tag="OO609_002080"
                     /gene_synonym="yjcD"
     CDS             complement(23688..25037)
                     /gene="ghxP"
                     /locus_tag="OO609_002080"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6633494.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25743..26207)
                     /gene="soxR"
                     /locus_tag="OO609_002081"
     CDS             complement(25743..26207)
                     /gene="soxR"
                     /locus_tag="OO609_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6633495.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26293..26616
                     /gene="soxS"
                     /locus_tag="OO609_002082"
     CDS             26293..26616
                     /gene="soxS"
                     /locus_tag="OO609_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6633496.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26619..28196)
                     /gene="pdeC"
                     /locus_tag="OO609_002083"
                     /pseudo
     CDS             complement(26619..28196)
                     /gene="pdeC"
                     /locus_tag="OO609_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28625..28906
                     /locus_tag="OO609_002084"
     CDS             28625..28906
                     /locus_tag="OO609_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6633497.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7253 a         7747 c         7328 g         6589 t
ORIGIN      
        1 agcacctcgc agtaaattcc ctgctctgtt agtggttgcg ttggcgctgg ttgcccttgt
       61 tttcgttatc tggcgcgtag acagtgcgcc atcaactaat gacgcttacg cgtcagcaga
      121 taccattgat gtggtgccgg aagtcagcgg tcgcattgta gaactggcgg tcaccgacaa
      181 ccaggcggtg aaacagggcg atttgctgtt ccgcatcgac ccgcgcccgt acgaagccaa
      241 cctggcgaaa gccgaagcct ccctcgcggc gctggataag caaattatgc tcacccagcg
      301 tagcgttgac gcccaacagt ttggtgccga ctcggttaat gccacggtag aaaaagcccg
      361 tgccgccgcg aaacaggcca cagatacatt acgccgcacc gagccattac tgaaagaggg
      421 ttttgtctca gcagaagacg ttgaccgtgc aagaacggcg cagcgcgccg cagaagcgga
      481 tcttaatgcc gtattgttac aggcgcagtc agccgccagc gccgtcagcg gcgtcgatgc
      541 gctggtggcg caacgtgcgg cggtcgaagc ggatattgcc ctgaccaaac tacatctgga
      601 aatgacgacc gttcgcgcgc catttgatgg ccggatcatt tccctcaaaa cctccgtcgg
      661 gcaatttgct tctgccatgc gccctatttt taccctcatc gacactcgtc actggtacgt
      721 gatcgccaac ttccgcgaaa ccgatctgaa aaatattcgc tcaggtacac ccgcaacgat
      781 tcgcctgatg agtgacagcg gcaaaacctt cgagggtaaa gtggattcga ttggctacgg
      841 cgtgctaccg gatgacggcg gcctggtgct gggcggcctg ccaaaagtgt ctcgttctat
      901 taactgggtc cgcgttgccc agcgttttcc ggtcaaaatc atggtcgata aacctgaccc
      961 ggaaatgttc cgcatcggcg cttcggcagt cgctaatctt gagccgcaat aatgagcgcg
     1021 ctcaactccc tgccattacc ggtggtcagg ctgctggcgt tctttcatga agagttaagc
     1081 gagcggcgac ccggccgcgt gccgcagatc gtgcaactct gggtaggctg cctgctggtg
     1141 attctgatct cgatgacttt tgagatccct tttgtggcgt tatcgctggc agtgctgttt
     1201 tacggtattc agtcgaacgc gttttacacc aaatttgtgg cgatcttgtt tgtggttgcc
     1261 acggtgctgg agatcggcag cctgtttttg atctacaaat ggtcatacgg cgaaccgttg
     1321 atccgattga tcatcgccgg gccgatcctg atgggctgca tgtttttgat gcgcacccat
     1381 cggctgggac tggtcttttt cgctgtcgcc atttacgggc aaaccttccc cgccatgctc
     1441 gactatccgg aagtagtcgt gcgcttaacg ctgtggtgta tcgttgttgg cctctatcca
     1501 accttgctga tgacgttaat cggcgtgctg tggtttccca atcgtgccat tacgcaaatg
     1561 catcaagcgc ttaatgatcg gcttgatgat gccattagcc acctgacgga cagcctcgca
     1621 ccgctacccg aaacgcggat tgaaagagag gcgctggcgc tgcaaaaact gaatgtcttt
     1681 tgtctcgcgg acgatgccaa ctggcgaact caaagcgcat ggtggcaaag ctgcgtggca
     1741 acggtaacct acatttactc gacgctgaat cgctacgatc ccacctcttt tgctgattct
     1801 caggcaatta ttgaatttcg gcaaaaatta gcctcagaaa tcaacaagct gcagcatgcc
     1861 gttgctgaag gtcagtgctg gcaaagcgac tggcggctca gtgaaagtga agcggtggcg
     1921 gcacgggaat gtaacctgga gaatatctgc cagacgttgt tacaactggg ccagatgaac
     1981 ccggatacgc cgccaacgcc cgccgccaaa ccgccatcaa tggtcgccga tgcttttacc
     2041 aatccagact atatacgcta cgcggtaaaa acattgctcg cctgtttgat ctgttacacc
     2101 ttttacagcg gcgtggactg ggaaggcatt cacacctgta tgctgacctg cgtgatcgtc
     2161 gctaatccaa atgtcggttc gtcgtaccag aagatggtgc tgcgttttgg cggggccttt
     2221 tgcggcgcga ttctggcgct gttattcacg ctactggtca tgccctggct ggacaatatt
     2281 gtcgaattgc tgtttgtgct ggcaccgatt ttcctgttgg gcgcatggat tgccaccagc
     2341 tctgaacgct cttcttatat cggcacacag atggtggtca ccttcgcgct cgccacgctc
     2401 gaaaacgttt ttggcccagt gtacgacctg gtggaaattc gcgatcgcgc cctgggtatc
     2461 atcattggta ccgtggtgtc cgcgatgatt tacacctttg tctggcctga aagtgaagcg
     2521 cgcacgctgc cgcaaaaact ggctggcgcg ctgggtatgt taagtaaagt aatgcggatc
     2581 ccacgccagc aggaagtcac ggctctgcgc acttatctgc aaattcgtat cggtctgcat
     2641 gcggcgttta atgcctgtga agagatgtgc caacgcgtgg cgctggagcg tcaactggac
     2701 agcgaagaac gcgccttact gattgaacgt tcgcaaacgg ttattcgtca gggccgcgat
     2761 atccttcacg cctgggatgc cacctggaat tcggcgcagg cgctggataa cgcactacag
     2821 ccggacagag caggtcagtt tgccgacgcc ctggagaaat acgctgccgg tctggcaacc
     2881 gcactcagcc gttctcctca aataacgctt gaagagacac ccacctcgca ggccatcctg
     2941 cccaccttat taaaacagga gcaacacgtc tgccagcttt tcgcccgctt gccagactgg
     3001 acagccccgg cattaacgcc cgccacggaa caggcacaag gagccacgca atgatcaatc
     3061 gtcaactttc acgtctgctg ttgtgcagca ttctcggcag cacgacgctg atttccggct
     3121 gtgccctggt acgtaaggat tctgcgcctc atcaacagct caaaccggaa caaatcaaac
     3181 tggccgatga tattcatctt gccagctccg gctggccgca ggcgcagtgg tggaaacaac
     3241 tcaatgaccc gcagttggat gccctgatcc aacggacgct aagtggttca cacaccctcg
     3301 ccgaagcgaa actgcgggaa gaaaaagcgc aatcgcaggc cgatttgtta gatgccggtt
     3361 cacagttgca ggtggcagcg ttagggatgc ttaaccgcca acgtgtctcg gcgaacggct
     3421 ttttaagccc ttatgcgatg gatgcgcccg cactggggat ggacgggccg tactatacgg
     3481 aagccacagt aggtttgttt gccggactgg atcttgattt gtggggtgtg catcgctcag
     3541 cggttgccgc cgccattggc gcgcataatg ccgcgctggc agaaaccgca gcagtagagc
     3601 tatcgctgac cacgggcgta gcgcagcttt attacagtat gcaggccagc tatcagatgc
     3661 tcgatctgtt agaacaaact cgcgatgtga ttgattacgc ggtgaaagcg caccaaagta
     3721 aagtggcgca cggtctggaa gcacaagtgc ctttccacgg cgcgcgggca cagattctgg
     3781 cggtcgataa acaaattgct gccgtcaaag ggcaaatcac cgaaacgaga gaatctctgc
     3841 gcgcattgat tggcgcggga gccagcgata tgccggagat caaacctgtg gcattaccac
     3901 gagtccagac cggcattccg gcgacactct cttatgagtt gctcgccaga cgcccggatc
     3961 tgcaagccat gcgctggtat gttcaggcgt cattagatca ggtggattcc gcgcgggcgt
     4021 tgttctatcc gagctttgat atcaaagcgt ttttcggcct tgatgccatc cacctggata
     4081 ccttattcaa aaaaaccagt cgccagttca acttcatccc gggtctgaaa ttgccgctgt
     4141 ttgacggtgg acggttgaat gccaatctcg aaggcacgcg cgccgccagc aacatgatga
     4201 ttgaacgtta caaccagtca gtactgaacg cggtgcgtga cgttgccgtc aacggcacgc
     4261 gtctgcaaac gctcaacgac gagcgagaaa tgcaggctga acacgtggaa gcaacgcgct
     4321 tcacccagcg cgctgccgag gccgcctatc agcgcggctt aaccagccgc ttacaggcca
     4381 ctgaagcccg gttgccagta cttgccgaag agatgtcatt actgatgctg gacagccgcc
     4441 gggtgatcca aagcattcag ttgatgaaat cgctgggcgg cgggtatcag gcggctcccg
     4501 tcgtcgagaa aaaataaaat gtctgccgcg tgatggctgt cacgcggtat ttcgtttcgt
     4561 cacgtcaaaa ctgacgacag cctgtttttc gtcagagttt tgaataaata gtgcccgtaa
     4621 tatcagggaa tgaccccaaa taaaatgtgg cataaaagat gcatactgta gtcgagagcg
     4681 cgtatgcgtg atttgattaa ctggagcgag accgatgaaa aaagtcgtca cggtttgccc
     4741 ctattgcgca tcaggttgca aaatcaacct ggtcgtcgat aacggcaaaa tcgtccgggc
     4801 ggaggcagcg caggggaaaa ccaaccaggg taccctgtgt ctgaagggtt attatggctg
     4861 ggatttcatt aacgataccc agatcctgac cccgcgcctg aaaaccccca tgatccgtcg
     4921 ccagcgtggc ggcaaactcg aacctgtttc ctgagatgag gcactgaatt acgttgccga
     4981 gcgcctgagc gccatcaaag agaagtacgg tccggatgcc atccagacga ccggctcctc
     5041 gcgtggtacg ggtaacgaaa ccaactatgt aatgcaaaaa tttgcgcgcg ccgttattgg
     5101 taccaataac gttgactgct gcgctcgtgt ctgacacggc ccatcggttg caggtctgca
     5161 ccaatcggtc ggtaatggcg caatgagcaa tgctattaac gaaattgata ataccgattt
     5221 agtgttcgtt ttcgggtaca acccggcgga ttcccaccca atcgtggcga atcacgtaat
     5281 taacgctaaa cgtaacgggg cgaaaattat cgtctgcgat ccgcgcaaaa ttgaaaccgc
     5341 gcgcattgct gacatgcaca ttgcactgaa aaacggctcg aacatcgcgc tgttgaatgc
     5401 gatgggccat gtcattattg aagaaaatct gtacgacaaa gcgttcgtcg cttcccgtac
     5461 agaaggcttt gaagagtatc gtaaaatcgt tgaaggctac acgccggagt cggttgaaga
     5521 tatcaccggt gtcagcgcca gtgagattcg tcaggcggca cggatgtatg cccaggcgaa
     5581 aagcgccgcc atcctgtggg gcatgggtgt aacccagttc taccagggcg tggaaaccgt
     5641 gcgttctctg accagcctcg cgatgctgac cagtaacctc ggtaagccgc atgcgggtgt
     5701 aaacccggtt cgtggtcaga acaacgtgca gggtgcctgc gatatgggcg cgctgccgga
     5761 tacgtatccg ggataccagt acgtgaaaga tccggctaac cgcgaaaaat tcgccaaagc
     5821 ctggggcgtg gaaagcctgc ctgctcatac cggctatcgc atcagcgagc tgccgcaccg
     5881 cgcagcgcat ggcgaagtgc gtgccgcgta cattatgggc gaagatccgc tgcaaactga
     5941 tgcggagctg tcggcagtac gtaaagcctt tgaagatctg gaactggtca tcgttcagga
     6001 catctttata accaaaaccg cgtcggcggc ggatgtcatt ttgccgtcaa cgtcgtgggg
     6061 cgagcatgaa ggcgtgttta ctgcggctga ccgtggcttc cagcgtttct tcaaagcagt
     6121 tgaaccgaaa tgggatctga aaacggactg gcaaatcatc agtgaaatcg ccacccgtat
     6181 gggttatccg atgcactaca acaacaccca ggagatctgg gatgagttgc gtcatctgtg
     6241 cccggatttc tacggtgcga cttacgagaa aatgggcgaa ctgggcttca ttcagtggcc
     6301 ttgccgcgat acttcagatg ccgatcaggg gacttcttat ctgtttaaag agaagtttga
     6361 taccccgaac ggtctggcgc agttcttcac ctgcgactgg gtagcgccaa tcgacaaact
     6421 caccgacgag tacccgatgg tactgtcaac ggtgcgtgaa gttggtcact actcttgccg
     6481 ttcgatgacc ggtaactgtg cggcactggc ggcgctggct gatgaacctg gctacgcaca
     6541 aatcaatacc gaagacgcca aacgtctggg tattgaagat gaggcattgg tttgagtgca
     6601 ctcgcgtaaa ggcaaaatta tcacccgtgc gcaggtcagc gatcgtccga acaaaggggc
     6661 gatttacatg acctaccagt ggtggattgg tgcctgtaac gagctggtta ccgaaagctt
     6721 aagcccgatt acgaaaacgc cggaatacaa atactgcgcc gtgcgcgtcg agccgatctc
     6781 cgatcagcgc gccgccgagc agtacgtgat tgacgagtac aacaagttga aaactcgcct
     6841 gcgcgaagcg gcactggcgt aatacagtcc tttctacagc ctcctttcgg aggctgtttt
     6901 tttatccatt cgaactcttt atactggtta ctccctaccc aatcgtatta tcaaaatgaa
     6961 aaaaattatc gcattgatgt tgtttttgac attctttgcc cacgcgaacg actccgagcc
     7021 tggcagccag tatttaaagg cagcagaggc cggggaccga cgtgcacaat attttcttgc
     7081 cgacagctgg tttagctccg gcgatttgag caaagccgaa tattgggcac agaaagccgc
     7141 cgacagcggt gatgctgatg cctgcgcgct gttggcgcag atcaaaatca ccaatccggt
     7201 cagtctggac tatccacaag caaaagttct tgcagagaaa gcggcgcaag cgggcagtaa
     7261 agaaggtgaa gtaacgctgg cgcatattct ggtaaatact caagcgggta aaccggatta
     7321 tccaaaggcg atttcgctgt tagaaaacgc ctcggaagat ctggagaatg actccgccgt
     7381 cgatgcccaa atgctgcttg gtttgattta cgccaacggc gtgggcatta aggccgacga
     7441 tgacaaggca acctggtatt tcaaacgcag ctctgcaatt tcccgaaccg gttattccga
     7501 gtactgggcg ggaatgatgt tcttaaacgg tgaggaaggt tttatcgaga agaacaaaca
     7561 aaaggcgctg cactggttga acctgagctg tatggaaggg tttgataccg ggtgtgaaga
     7621 gtttgaaaaa ttaaccaacg gttaaggtag gcctgataag acgcggcaag cgtcgcatca
     7681 ggcatccaca cattgccggg ggatatcccc ggcaatcttc aattattgat ccgcagtttt
     7741 atcaaatttg cccagcactt cacgctcata agccagcgcc tttttgcggt caaatttgtg
     7801 ttcccacttg gcaatcacca gcaccgccag cgcattaccc accacgttca gcgcagtacg
     7861 cgccatgtcg aggatacggt caacaccagc aataaacgcc agaccttcca gcggaatacc
     7921 tacgctaccc agcgttgcca gcaacaccac aaacgacacg ccagccacgc cagcaatccc
     7981 tttcgaggtc accatcagcg tcagcaccag aatgatttcc tgccagatgg acaggtcaat
     8041 gccatacagc tgagcgatga aaatagcggc aatactttga tacagcgtcg aaccatcaag
     8101 gttaaaagag taaccggtcg gcaccacgaa gctggtgatc gacgccggtg ctccgtaggc
     8161 ttccatcttc tcaataattc gcggcagtac gctttcagag ctggcagtgg agtacgccag
     8221 aatcagctca tctttcagaa tacgaatcag gatccagacg cttaacccgc acagacgtgc
     8281 cacaattccc agcactacca gcgcgaagaa cagaatggcg aaatgcacca gcagcaccag
     8341 tttcgccagt ggccacagag acgagaaacc aaagttagcc accgtcaccg caatcagcgc
     8401 aaacacaccc accggtgcat aacgcatcac catgtgagtc actttaaaca tggtttcaga
     8461 gatggagcgg aacacggtca ccagcggttc acgatgcgtc gcgggcaggg aagaaagccc
     8521 cagaccaaac agcaccgaga aaaagatgat cggcagcatt tcgcctttcg ccatcgacgc
     8581 cacaatgttc gtcggcacca gcgacaaaat cgtgcccata atgccgtggg aactgctttg
     8641 taccgcttcc gtagtgctct gatatttcga gatatcgacg gtcgccagct gcgacatatc
     8701 caccccggca ccgggctgga agacgttcgc cagagtgatc cccaaaatga tggcgacggt
     8761 ggtgatcacc tcgaagtaaa taatggtttt cgcgccaata cgcccgagct gtttggcatc
     8821 accaacaccc gcgataccca ccaccagcgt ggagatcaca atcggcacaa caatcatttt
     8881 gatcagatgg atgaagatat cacccgccgg agagagcaaa ttgacgacca gccagtcgcg
     8941 gctgtcgcta tggtagtgca ggtagcttcc caggagaatg cccagcacca tagcaaacag
     9001 aatctgccag gccaggctga attttatatt tttcatcaga ataacttcct caatggagcg
     9061 ccgttccccg ttcagaaacg cgagaacgtg acatactgcc agggcatgaa ttgtgttgcg
     9121 ttggtttgta gattttttta acgcttatga tcagtatcat ttgcgcggta tgttgcgcaa
     9181 ccctttaaca acagagcttt tcgctgcatt aatgcataat aacgcgagtt tatggcagga
     9241 ttaatgaata accatttgtt atggaaagat ttttatttgt ttaatctgca ataatactga
     9301 ttggtgatta tttttggcat atgttcattc gattattttt tatgcagctc aatgacataa
     9361 tcttcatcac cagatgatta atccgatctc cgctgcaaca gttttgccat attaatcgac
     9421 tcgaccagct gtgtccggtt gatccggggt gagttgagat ccatcacttt ttgccatagt
     9481 tcgatggctt gcgcgtaatt cgcctgcata aacgcatcgg aagccagcag catcagagcg
     9541 gtgatttcgt tagagtccag cgcgagggct ttgtcgatca ttgcgcgagt ctgggcggtc
     9601 atatgctggc tggcctggta ataaagcacc gtcgccagcg ccgcatacag ttcagcgttc
     9661 tcaccacgta gttgcaacgc ctgacgatac gccagcagcg aattgctgta atcgttttgc
     9721 cacagatagt actcgcccag taacgcccat tgttcgctgt tttgtggatt agcgcggatt
     9781 ttatcctgca atgcctgaag ctgcgcttcc ggggtttgct ggctggcaaa ctgatgtagc
     9841 ggatcgcgct gacgctgata ctccgcacgt accgcctgcc attttgggct taacagataa
     9901 ctgccgacac agaggaatac catcaatatc gtgagtgttg tcagcatttt caccggtatt
     9961 tgaggctgtt tcatcgctta gccctcactc gccagaggat cagtgccatc agcaataaca
    10021 acaccactgg cagcgcccac aacaccagcg tctgacccgt taacggtggg ttatagcgaa
    10081 caaaatctcc gtagcgttcg gtcatccagc caatgatttc gacttcgttc ttcccctccg
    10141 acaccatgct ataaacctga tggcgcatac tgaccgccac cggcgcattg gattcgagta
    10201 agttttgatt ctggcactgc ggacaacgta actggctggc aatatttaac gcctgttgct
    10261 gttgttgcgg attggcgaat tgccaggtgt ctacaacctg agcgcgcgca aaacaggtaa
    10321 ataacagcag tagcgtgaga agccctttat tcatcgcgct tcttcccccg ccatccgctt
    10381 aacaatgcgc ccgcaatcat caacaatcct cccccccaga tccagcgcac accgctttgt
    10441 acatacaaac gaaaagcgta acgatccgct ctggtttttt cacccatgac cgcataccag
    10501 tcatggatgc cgttccagcg aattgacggt tccatcattt gctgacggcg tgcttcataa
    10561 aaacgccgct ccggcgttaa ttcaccaatg cgttgctgat ggtcaaacag cgccactatc
    10621 gctttttcgc tggtgtaatt gcctctggct tgcagatcga gtcgctcaaa acggaaggtg
    10681 tatcctgcca gcgtcacctg ctgacccggc tgtaaattca ggctgatctc ctgacgactg
    10741 acactggaaa ccacgatccc cgcggcaaat aacagcacgc cagcatgagc taccagcgcc
    10801 ggaagctgca cgcgtttgcc agagacaaac gtcgccagca caatcaccac cagcatcaac
    10861 agaccaaacg gtaacgtcgc gcggttaaaa tacggcgcgc caacggagag gcgtccccag
    10921 cccagcaggc cgtaaatcat cggataaagc gttcccacca gcacgatcag tagcactgcg
    10981 caaaacagca acagcgtagc gaggattaac atttcccgcg ataaccccga aaaacgcacc
    11041 gccgggccac cgtcccgcgc tcgccagcca tacagagcca aagacgcaag gctaatcagt
    11101 gcaaacaggc tgaacaacgg cacggcgcgg acgttatcca gtgcgaacgc atgaaccgaa
    11161 accagaatgc cagaacggac aattaaggtg cccagcagcg acagcatcag agtaactatc
    11221 gccagcaaca gcgaccagtg gcggaaaatc ccccgctggc gtgtcaggga caaactgtgc
    11281 agcagcgcag tggcagaaag ccagggtaat aaagaggcgt tttccaccgg atcccagaac
    11341 caccagccgc cccagccgag ttcgcagtag gcccaccagg aaccgaggat gatccccgcc
    11401 gttaatgcac tccagccagg taacgcccag cgccagcaaa ttcgggcgct ggccgcatca
    11461 aactcgccgc gcagtagact cgccagcgcc acgctcgccg ctaccatcaa accgccatag
    11521 ccaaggtaaa gcagcggtgg atgaaagata agaccgggat gttgcagcat cggattgaga
    11581 tcgcggcctt cgattgctgg tggaaatatc cgcacgaagg gatcggacca cagcaccaca
    11641 aacagtagca gtgcggcgag catgagagat aaaacggcta gcgtcagcgg aaatagcgga
    11701 tcgctttgct gccgataatg ccaggcaaac agcgcgctcc aggcggaaag cagcagcacc
    11761 caaagcagca gcgaaccttc atgaccgccc cacaccgccg ccagtttgag ttcccacgac
    11821 agcaggctgt agctatgttg ggcgacataa atgaccgaga aatcgctgat gagaaaacaa
    11881 tacgtcagta cgccaaaggc gagcagcagg agcgcgaact gcgccagaat gccgatgcaa
    11941 gtgagtcgca tcatggcagg ccagcgcaac cgcactcccg caaaggccgt caacggggtc
    12001 aacacgttga ccccgagact taacaacaac gccagaaaac cgacttcagg aaggaatacg
    12061 tccagaaacc acctcttttc tggcttacgc caccgtcaac tgtccggcgt agagaatgaa
    12121 aaagcgcagc atcaacacgc cgaccagact ggccccacac gccgccagca cggcaggaat
    12181 gccggaactg cgattgaccc acggtttgag caacattggc acaatcagcc ccagcccggc
    12241 gacaccaagc cagaaccacc acgtccagaa accgccacct aatgccgcca ccagcgcacg
    12301 aactttaccg tcatcgccca gcgccagacc gacaaaaaac gccaccagca ggaagatttc
    12361 accccatacc acggggattt ccatccggtg aacaaagtgc gcttccgtgg aatgcgggtt
    12421 actgcgttgg cgtatcgcca tggcgatcag cgccaccgcc gcaccggacg agatgccgga
    12481 gaagaggagc agcaccggca ggctcgggtt attgaggaac ggatacgatt tcagcgccga
    12541 cagcagaaag ccggtataag cccccagcag caccgccaac accagcatca gcgtttccag
    12601 tccgcgatga acgggcgtca gcaggctcag aaccttttgc acgatcccca gcttcggcaa
    12661 ccagcgttgt tgcagggcaa gcaaatcatg ttcaaagatt ttcgccagcc acagcaccag
    12721 caccaccatg tagagctgaa acagcatcac ccccatcgac atcaccgagg taaaactgta
    12781 gtggaacatc agcttccaga aggtccacgg tcttgtcagg tggaagacga ggatcaacag
    12841 accgaggatc accgcgcccg gcccgacaat cagcgtggtg cgcagcaacg tactgtctgc
    12901 accgcccgcc tgcgggtaga agcgacgtaa cagcacggcc agcgtcacca gaccggcaga
    12961 aataccaatc aaaaacaggt agatggcaat cggccagtcc cacaccagcg attcaaaatg
    13021 aaatgcggaa gtctgcgtca ttggctcacc tccccgtatt taaacggtac gcggtacagc
    13081 ttcggtttgg ttcccagcgc cagcttgtag cggtaagtag gcttctggcg cagcagttgc
    13141 gaaatctcac tgttaggatc gtccagattg ccaaacgtca gcgccttcgt cgggcaagct
    13201 tcaacgcacg cgggaagctt accggcctgc aaattggtct tacgacagaa atcgcatttg
    13261 tccgccgtct tcgtgaccgg atggataaag cgcacgcggt acggacaggc ggcgatgcag
    13321 tactgacaac cgacgcagag atcaggatta acatcgacaa tcccgctagc ggcatcacga
    13381 aaagacgcac cggtcgggca gacgtcaacg cacggcgcat gatcgcagtg ctggcaagac
    13441 ttacggaaga agcgatattt cacatcagga aattcgccct gcggctcgct acgaataatc
    13501 gtcaggcgcg agacgccttc cggcaccttg ttcacttccc gacaagcatc catacaggcg
    13561 gtacagccga tgcataacga ctcgtcatgc accatgccgt aacgcacccc attgatattc
    13621 aacgttttcg ccaccacgcg ccctgcggtg ccgctgacgg ctgccagcac gccgacgccg
    13681 gtgagaaact ggcgacgaga ccaggtcatg gctgctcctt aagcaacgga acagacgccg
    13741 ggttaaagtt cggattggtg cgctgatcgc tgtggcaatc gacgcaaatc ttaatccgtc
    13801 ctttgtcact taacgtctgc atcgtatctt gctgtggatg cagggaatgg cagctggcgc
    13861 acgccacttt ggttacgtgg acatcgtgcg gccagaacgc tttttgcaac tgttccggca
    13921 gatgacagga catacagacg ctgttctgct ctccgacctt atacatcggc tcgttaaagc
    13981 gcatcacatc tttcacccct tcgcggtgtt gcggtgatgc ttggccgtga cagttggtgc
    14041 aggtgaccgg caatttgtta ttcgggttga taacggaagc atgtttgcca tgcatacctt
    14101 cggtatctgg tttatgacag tccagacagg cggcatccgg gttacgctgc tgggtaactt
    14161 cgtagcgatc accagatgcc tgcaccgcag gggtagcggt aattccgttc aggctccaca
    14221 acaggcccga cgccagcacc ccagcagtta ataacgaacg taatacgctc atattcactc
    14281 cattgtgttt tccgcccctt gcgaggcgga acggggttat tggcttaaca gaccgttttt
    14341 acgtgcctgc tcttcccact gcgggatcac cgttttgatg aagtcctgct tctcggcctt
    14401 gatttgttcc atgttcaggc caatggcctg ctgggctttc tctttggtcg agatatccgg
    14461 gatctggatt tcatgggtga tacctttggt cgccagcagg cgcgccagtt tggtgcgcgc
    14521 atccgccgct ttatccatcg ccgtaccgag catccgtaga ccttcttccg gtgcgtgcat
    14581 atgaataccg tgggaagcaa tcgccagatc ccaacgccac tgggcatgac ggatatcgtc
    14641 ctgaatcggc ttcatttcgg cttccgtcgc gcctgcatcc agcgccgctt tcgcttcgaa
    14701 gtgagcgtga accagttgat cttcaacctt gattttcagg tcgttaatcg attgcttacg
    14761 ttccgcgacc actttttgca aggcagcttt atcctgggta tggcagttcg cacaagtctg
    14821 ggcgaagtta tcaaacggat taccaatttt atggtcggtg tagagtttgc cttcggcgtt
    14881 ctgcactttt ggcatatggc agtcgataca ggtcacgttg tttttaccgt gaatgcccgc
    14941 tgtccaggtt tcatattccg ggtgctgcgc tttcagcatt ggcgttttcg acagggagtt
    15001 agtccagtca gagaaggcaa ttttgtcgta atactgctcc atattttcga ctttcatgcc
    15061 gtcatcccac gggaatttaa ccgctttgtt tttgccgtcg aagtaatact ccacatggca
    15121 ctgaccgcaa accatcgatt gctggtcgaa acgtccggct ttctcaaaag gtttaccaat
    15181 ggcttccatc gcgcgagccg catacggacg ggaaagggtt aactccggtt tgcctttggc
    15241 gaactctggt gaggcggtgt tatggcagtc ggcacaacct aagttgttga cgatttccgg
    15301 gccgccgcgc gcccatttac cgtggaagta gccatcttcg ccgtctttct ggatcagacg
    15361 cgccacatcc gggcttttac aactccagca cgccatcggt agcggaccat cttcagcgtt
    15421 tttcggcgca ccggtacgca gggtttcacg cacatcggtc acagcaaagg catgtccacg
    15481 aggcttgttg taatcgcgcg agaagggata ccccgcccac aggatcacca gccgtggatc
    15541 ttccgccagg gcgtcaacac gctctgactg ttccgaggtg gctttccagg agagatattg
    15601 atcgtgatgc tgcggggcaa aggtttcatt cttcgcttcc acagttacag gttttgcggg
    15661 agctgccgtt tgttcagcgt gaacagaagt gaaaaagaaa aaaggaatca ataagctgaa
    15721 gatacggcgt gcgtttattt ttatccttgt cataggggct tcatccgaat tgcgccattg
    15781 ttgcaatggc ggtttttatt gtttttcacg acagtaaccg cacctacact gtcatgacat
    15841 tgctcacccc tatgtgtaac aaataaccac actgtgaatg ttgtctttaa tcaattgtaa
    15901 gtgcatgtga aataccactt tagagttagt cagtatcttc ctctttttca acagcatgca
    15961 taactgcatg ttcctcaaag aattaatcaa cttttgttgg tgaccttcaa aaattaccct
    16021 gccgtttatt tgcaccattc tacttttgcg tgatctgtcg cccaaatact aaacaaaact
    16081 gccaataccc ctacatttaa cgcttatgcc acatattatt aacatcctac aaggagaaca
    16141 aaagcatgag ccaaattcac aaacacacca ttcctgccaa catcgcagac cgttgcctga
    16201 taaaccctca gcagtacgag gcgatgtatc aacaatctat taacgtacct gataccttct
    16261 ggggcgaaca gggaaaaatt ctcgactgga tcaaaccgta ccagaaggtg aaaaatacct
    16321 cctttgcccc cggtaatgtg tccattaaat ggtacgagga cggcacgctg aatctggcgg
    16381 caaactgcct tgaccgccat ctgcaagaaa acggcgatcg caccgccatc atctgggaag
    16441 gcgacgacgc cagccagagc aaacatatca gctataaaga gctgcaccgc gacgtctgcc
    16501 gcttcgccaa taccctgctc gagctgggca ttaaaaaagg tgatgtggtg gcgatttata
    16561 tgccgatggt gccggaagcc gcggttgcga tgctggcctg cgcccgcatt ggcgcgatgc
    16621 attcggtgat tttcggcggc ttctcgccgg aagccgttgc cgggcgcatt attgattcca
    16681 actcacgact ggtgatcact tccgacgaag gcgtgcgcgc cgggcgtagt attccgctga
    16741 agaaaaacgt tgatgacgca ctaaaaaacc cgaacgtcac cagcgtagag catgtggtgg
    16801 tactgaagcg tactggcgga aacatcgact ggcaggaagg gcgcgacctg tggtggcacg
    16861 acctggttga gcaagccagc gatcagcact aggcggaaga gatgaacgcc gaagatccgc
    16921 tgtttattct ctatacctcc ggttctaccg gaaaaccaaa aggcgtactg cacactaccg
    16981 gcggttatct ggtgtacgcg gcgctgacct ttaaatatgt ctttgattat catccgggtg
    17041 atatctactg gtgcaccgcc gatgtgggct gggttaccgg acacagttat ttgctgtacg
    17101 gcccgctggc ctgcggcgca accacgctga tgtttgaagg cgtaccgaac tggccgacgc
    17161 ctgcccgtat ggcgcaggtg gtggacaagc atcaggtcaa tattctctat accgcaccca
    17221 cggcgatccg cgcgctgatg gcggaaggcg ataaagcgat tgaaggcacc gaccgttcgt
    17281 cgctacgcat tctcggttcc gtgggcgagc caattaaccc ggaagcgtgg gagtggtact
    17341 ggaaaaaaat cggcaacgag aaatgtccgg tggtcgatac ctggtggcag accgaaaccg
    17401 gcggtttcat gatcaccccg ctgcctggcg ctaccgagct gaaagccggt tcggcaacac
    17461 gtccgttctt cggcgtgcaa ccggcgctgg tcgataacga aggtaacccg ctggaagggg
    17521 ctaccgaagg tagcctggtg atcaccgact cctggcctgg tcaggcgcgt acgctgtttg
    17581 gcgatcacga acgttttgag cagacctatt tttccacctt caaaaatatg tatttcagcg
    17641 gcgacggcgc gcgtcgcgat gaagatggct attactggat caccgggcgt gtggacgacg
    17701 tgctgaacgt ctccggtcac cgtctgggga cggcagagat agagtcggcg ctggtggcgc
    17761 atccgaagat tgccgaagcc gccgtagtag gtattccgca caatattaaa ggtcaggcga
    17821 tctacgccta tgtcacgctt aatcacgggg aggaaccgtc accagaactg tacgcagaag
    17881 tccgcaactg ggtgcgtaaa gagattggcc cgctggcgac gccagacgtg ctgcactgga
    17941 ccgactccct gcctaaaacc cgctccggca aaattatgcg ccgtattctg cgcaaaattg
    18001 cggcgggcga taccagcaac ctgggcgata cctcgacgct tgccgatcct ggcgtggtcg
    18061 agaagctgct tgaagagaag caggctatcg cgatgccatc gtaacccaca attgccggat
    18121 gcgaatcggt aacggtttgt aggcctgata agacgcgaca gcgtcgcatc aggcattgat
    18181 tgccggatgc ggcgtaaacg ccttatccgg cctacattcg gcaagggtta cccgagcgtt
    18241 aaccttctcc cataagggag cgggaattaa aacaatccct acattacctc tggagaatct
    18301 gtgatgaatg gtacgattta tcagcggata gaagacaatg cgcatttcag ggagttagtc
    18361 gaaaaacggc aacggtttgc caccatcctg tcgattatta tgctggcagt ttatatcggc
    18421 tttattttac tgatcgcctt cgcgcccggc tggctgggca cgccgctgaa tccgaacacc
    18481 agcgtcacac gcggtattcc aattggtgtt ggagtgattg tgatctcctt tgttctcacc
    18541 ggtatctaca tctggcgggc gaacggcgaa ttcgaccgtc ttaataatga agtcctgcat
    18601 gaggtacaag catcatgaaa agagttctga cggcgcttgc cgccacactc cctttcgcag
    18661 ctaacgccgc ggatgctatt agcggggccg tagagcgcca gccaacgaac tggcaggcga
    18721 ttattatgtt cctgattttc gtcgtgttta cgctcggcat tacctactgg gcatcaaaac
    18781 gcgtacgttc tcgtagcgac tactacaccg caggcggcaa tatcactggc ttccagaacg
    18841 ggctggcgat tgccggggac tatatgtccg ccgcctcatt cttggggatc tccgcgctgg
    18901 tgtttacctc cggctatgac gggctgatct actcgttggg cttcctggtg ggctggccaa
    18961 tcattctgtt tctgattgcc gaacgtctgc gtaacctggg acgctacacc tctgccgatg
    19021 tggcctctta tcgtctgaaa caagggccga ttcgtattct ttcggcctgt ggttctctgg
    19081 tagtggtggc gctttacctt atcgctcaga tggtaggcgc aggtaaactg atcgagctgc
    19141 tgtttggcct taactatcac attgcggtgg tgctggtcgg cgtgctgatg atgatgtacg
    19201 tcctgttcgg cggcatgctg gcgaccacct gggtacaaat tatcaaagct gtgctgttgc
    19261 tgttcggtgc cagctttatg gcctttatgg tgatgaaaca cgtcggcttt agcttcaaca
    19321 atctgttcag cgaagcgatg gcggtacacc cgaaaggtgt cgacatcatg aaaccgggcg
    19381 gactggtgaa agatccgatc tccgcgctct ctctggggct gggactgatg tttggtacgg
    19441 cgggcttgcc gcacattctg atgcgcttct ttacagtcag cgatgcccgc gaagcacgta
    19501 agagcgtgtt ctacgccacc ggatttatgg gctacttcta tattctgacc tttattatcg
    19561 gcttcggcgc gatcatgctt gttggtgcga atccggaata taaagacgcg gcgggccatc
    19621 tgattggtgg taacaacatg gcggccgttc acctggcgaa tgcagtgggc ggcaacctat
    19681 tcctcggttt tatttcagcg gttgctttcg ccactatcct cgcggtggtt gcggatctga
    19741 cgctggcggg cgcatcggcg gtttcgcatg acttgtacgc taacgtcttc aaaaaaggcg
    19801 cgaccgaacg tgaagagctg cgggtatcaa aaatcaccgt actgatcctc ggcgtgattg
    19861 cgattatcct cggcgtgctg tttgagaatc agaacatcgc ctttatggta ggccttgcgt
    19921 ttgccatcgc ggcgagctgt aacttcccga tcattctgct ttccatgtac tggtcgaaac
    19981 tgactacccg tggcgcgatg ctgggcggct ggctggggct gattaccgca gtggtgctga
    20041 tgatcctcgg cccgactatt tgggtacaga tcctcggtca cgaaaaagcc atcttcccgt
    20101 atgaataccc ggcgctgttc tctatcagcg tggcattcct cggcatctgg ttcttctcgg
    20161 caaccgataa ctcagcggaa ggggcgcgcg agcgtgaact gttccgcgcg cagtttatcc
    20221 gctcccagac cggctttggc gttgagcaag gccgcgcaca ttaatctctc cccttccccg
    20281 gtcgcctgac cggggaatac gcttcctctc caacatgcat caccttttcc cataatatta
    20341 aacaaataaa ctcattaaaa aatgagcgat ttttgacagt cgtagaaaat gataatgcag
    20401 agaatatgcc ttttctttct tgttaattat aaggatattt tatgcgctat aatggtttaa
    20461 ataatatgtt tttccctctt tgccagatta acgataacca ctctatcaca agttcatcac
    20521 atacaaagaa aacaaaatca gataattaca gcaaacatca taaaaacacg ttaattgaca
    20581 ataaagccct ctctcttttc aaaatagatg atcatgaaaa agtgataggc ttgattcaga
    20641 aaatgaaaag aatttatgat agtttaccat caggaaaaat cacgaaagaa acggacagga
    20701 aaatacataa atattttata gatatagcgt tatatgcaaa taataaatgt gacgatagaa
    20761 ttacgagaag agtttacctt agtaaagaaa aggatgtatc cattaaggtg gtatatttta
    20821 taaataatgt cgccgtccat aataatacta tcgaaattcc acagatagta aatggtggtt
    20881 acgatttttc acaccttagc ctgaagggta tcgtgattaa agatgaagat ttatccaatt
    20941 cgaattttgc aggttgcaga ctacaaaacg ctattttcca ggactgtaat atgtataaaa
    21001 cgaattttta ttacgccata atggaaaaaa tactttttga taattgtatt ctcgatgact
    21061 caaatttcgc tcagataaaa atggccgacg gaactctaaa tgcatgctcc gctatgcatg
    21121 ttcaattcta caatgcagca atgaatagag ccaatattaa aaataccttc cttgactatt
    21181 caaattttta tatggcgtac atggctgagg taaatcttta taaagtaata gcgccatatg
    21241 ttaatttatt taaagccgac cttagtttct ctaaactcga tttaattaac tttgaacatg
    21301 ctgatctgtc tcgcgtcaat ctgaacaaag caatcctcca gaatataaac ttaattgata
    21361 gcaaactctt ttgtacgtgg ctgacaaata cattcctcga aatggttata tgtaccggct
    21421 ctaatatggc taatgttaat tttaataatg ccaatttaag caactgccat ttcaactgtt
    21481 ctattttaac aaaagcctgt atgtttaata cccgtctcta tcgggttaat ttcgatgagg
    21541 ctagcgtcca gggaatgggc atttccattc tccgtgggga ggaaaatatc cccattgata
    21601 gtgataccct ggtaacacta cagaaattct ttgaagaaga ttgtacctct catactggca
    21661 tgtcacaaac tgaggataat attaatgcag tcgctatgaa gattactgca gatattatgc
    21721 aacacgcaga ttgaggttaa gtcgaagaac agccaatgaa ttgactttac aagagagata
    21781 acttatttgt tttagaatca tgaatgttcg cccgagctaa cctgttaaag gtgcaaaaga
    21841 ttgcaccttt ttcagattac ctcgggcctg gttacgtcgc tttcggctta ctgattttcc
    21901 tcaattagca acgcttcaag caaatcgaga tcgtgcagta atttttgcag cgtttcgttg
    21961 ctgatctccc gcgtggcgcg caggtggtac agttcagcac gttcagaacg caatgccgcc
    22021 agacggaagc gacgctcaag gttctcttcc tgcacggaac tttcaacgtc gttacgtcca
    22081 tcggcgcgac gacgcaggtt accaatgacg cgagaactga cctccgtaag caactggtta
    22141 tcgatattct cttcggtatc cgccgccaga cgctcctcca ttttctggat cgccacaatc
    22201 gccacttctg ccgttgccgc tcgcgcaata cgttcttctt tcaattgttg cgaatggtcc
    22261 gcgacttcaa tgtgttgtag cagaataggc aacatcacca cgccgacaaa cagcgagaag
    22321 agaatgacgc cagccgccag gaacaccagc tcatagcgcg ccgggaagac gttaccatcc
    22381 ggcaagagca gcgggattga gagcacaccg gccagagtga tcgccccacg caccccagcg
    22441 aaagacgcga tcaggatttc tcgtgtggtc cacgaaccaa actccatcgg cttcttcttc
    22501 aggaagcggt tgctgaactt tttcatcgtc cacagccagc cgaaacggac cagcatcagc
    22561 gccgcatata tcagaataat atcggtaaac agcatccaga tttcgacgtt agggtcgatt
    22621 tctgccgcca tcagcgacgt ctccagaata cccggcagtt gcagacctaa cagcaggaac
    22681 accatgccgt taaagacaaa ttccagcatc gcccaggtgc tgtttgcgcg caggcgcatt
    22741 gccagtggcg cacggcgcat cacaccggag cgggtgatgg tcatcccggc ggcaaccgca
    22801 gcgaggatgc ccgaaacgcc aatatgttcg gcaatcagat aagaagcgaa tggcagcaag
    22861 aacagcagaa cgatctgcgt cgcgggttca tcaccgcccc agcggctgag gaatcgcagc
    22921 gagcgaccat acagccagct caccacaaaa ccggcaagaa taccgccaat ggctactttc
    22981 ataaattcga ccgtcgcacc gccgacagtg aagatcatcg tccccatcgc cactgccacg
    23041 gcaaacttca acgacaccag gccggaagcg tcattcatca acgcttcgcc ctgcaaaatg
    23101 cccatgattt ttttcgggat gcgcccttcg ccaacaatcc cggagagcgc cacagcatcg
    23161 gtcggagaaa gtaccgccgc cagcgcaaag gcggggatca gcggaatgcc cggcaccacc
    23221 cagtaaataa ggaagccaat gccgaccacg gtgaccacca ccagcgccag cgcgaggccg
    23281 aaaatctctc gaccatgttc gagaaattca cgggtcggcg ttttccagcc atcagcgaac
    23341 agcaacggcg ggataaacaa gactaaaaag agttcaggat caaactccac atgcaaacca
    23401 aacgtcggcc acgccagtag cgcaccgatg gcgatttgca taagcggaag cgggatctga
    23461 aagggcatga cacgagtgac caccccggac agcgagacca cgagggtcat tatcagtatg
    23521 gtgaagaaga tttccatgcg ttccctgttt gcgatgtttt ttatgatctg agaaagacgt
    23581 tatgcctgaa ttctcatttt ttcagaatat cgcaaaagct cacacaatgt gcggagaaaa
    23641 taaaaacggg cagccattgc cacccgtttt cagaatccga tgaaagatta gatagcccag
    23701 ccacccgcat agaaggtcac cagcgccacg gcgataacga ccgtaccgat gttcaacttg
    23761 cgccattcac cggaaaccag acgaccaatc accagagtcg cgaagccgat catgatgcct
    23821 gttacgatgt tacaggtcag cacgatgaat accgccgtaa ccagacccgc catcgcatca
    23881 acaaaatcag caaagtcgat tttcgccacg ttgctcagca tcagcaggcc aacgtacatc
    23941 agcgccggag ccgttgcata ccccggaacg aggtaagaaa gcggagagag gaacagaatc
    24001 agcaggaaca gcacgccaac ggtgatagcc gtcaacccgg ttttaccgcc cgccgccgta
    24061 cccgccgcag actcgatgta taccgctgcc ggagctgcac ccaccaggcc agagaaaacg
    24121 ctgctcatgg agtcagtggt cagtgctttc ccgccgtcga tgatctgccc atctttatcc
    24181 agcaggttcg cctggccggc gacagcgcgg atagttccgg tggcatcaaa tactgccgtc
    24241 atcaccagcg ccagaacgct tggcaggact acaggattca gcgcgcccat aatgtccagg
    24301 ctgccaatca gtgaattgcc gttttcatcg ctcaatgaag gcatggcgaa aacgccggag
    24361 aaatggacgt taggatcgaa gatcaaaccg acaattgaga taccgataat ggtcagcaga
    24421 atgccaccag ggactttcag tttttccaag ccgatgatca ccgccagacc tgccagtgac
    24481 ataatcaccg ggaaggtcgc gaaatcaccc agcgcaacgg gcagaccatc aagcgggttt
    24541 ttaatcacca gaccgacacc attagcggca atgagcagca ggaacagacc gataccgatc
    24601 cccgtgccgt gcgccacacc gtgcggcaag ttgcgcaaaa tccagctacg gatacccgtg
    24661 gcagaaatta ccgtaaacag cacacccatc aggaacacgg cacccagcgc gacaggtacg
    24721 ctaatatgtt gccccagcac caggctgaat gcggtaaacg ccgtcaggga gatggcgcaa
    24781 ccaatcgcca acggcagatt agcccacaga cccatcacga tagaaccgag tccggcaacc
    24841 agacaggttg caacgaaaac tgccgcaggc gggaagcctg ctttacccaa catacctgga
    24901 acgacgatga ccgagtagac catcgccaga aacgttgtta acccggcaac cacttcctga
    24961 cggacagtgc ttccacgttg tgaaatttta aaccaggcgt cgagtgaacc gccggtacgc
    25021 gctgatggcg tagacataag aaaacatccc ctgagagttt aattttcgtc aagatgcgtg
    25081 gcggacaatc ccctgccagc gaaacgttat cttcctgctc caggtttgcg gcaatttggt
    25141 gcgtcgcaaa aaagcaaacg tttagctccg cgcatacaaa acgggtggta aaaaaaagca
    25201 aacgattatc cggcgatcat agcggctttt tcaaccgaag tttgctgctt ttttctcttt
    25261 tattgtcttg acgatgaaaa accaggcaat tgatgcgcaa acgttatcgt cgatgtgcaa
    25321 gattgcagcg ttatcgagcc gtataccgtt ctggattgcg ataaaaaatc gtctgagatg
    25381 acaaaaatat tgcggctaaa aagtatactt ttacactggc gaagaaacgt cggtgacata
    25441 caattaaagc atcaacacca accggaacct ccaccacgtg ctcgaatgag gtgtgttgac
    25501 gtcgggggaa accctcctgt gttccagcgg gatagagaga aagacaaaga ccggaaaaca
    25561 aactaaagcg cccttgtggc gctttagttt tgtgtttgcg gataattcca aaagagctcg
    25621 atcagtcccc tcgccccttc ggggagaggg ttagggtgag gggaacaggc agcaccagtg
    25681 caaatattta ccctcacccc ggccccatag ttaacgatga gtcagcttgt ccttgtggcg
    25741 ctttagtttt gttcatcttc cagcaagcgt gcgccggtac cttcttctcc taagcggtcg
    25801 cccgggttac gcaacgggca atcactgcgc gaaaggcagc cacaaccaat acatccgtcc
    25861 agttcgtcac gcagcgccac taaggtatga atgcgccgat ccaactcttc tcgccattgg
    25921 gacgaaagct gtttccactc tttcgcactt aacgtatgcc cttctggcaa cacgccaaac
    25981 gcttcaccaa tggtcgccag cggaatgcca atacgctgag cgattttgat aattgcgaca
    26041 tatcgcaaca catcacgttt atatcgccgc tgattgccgc tgttacggat actagtaatc
    26101 aaccctttgc tttcatagaa atgcagcgcc gataccgcca caccgctgcg tttcgccact
    26161 tcgccagggg ttagcagcgc tttaatgcgg ggtaatttct tttccataaa tcgctttacc
    26221 tcaagttaac ttgaggaatt atactcccca acagatgaat taacgaactg aacactgaaa
    26281 agaggcagat ttatgtccca tcagaaaatt attcaggatc ttatcgcatg gattgacgtg
    26341 catattgacc agccgcttaa cattgatgtc gtcgcaaaaa aatcaggcta ttcaaagtgg
    26401 tacttgcaac ggatgttccg cacggtgacg catcagacgc ttggcgatta cattcgccaa
    26461 cgccgcctgt tactggccgc cgttgagttg cgcaccaccg agcgtccgat ttttgatatc
    26521 gcaatggacc tgggttatgt ctcgcagcag accttctccc gcgttttccg tcggcagttt
    26581 gatcgcactc ccagcgatta tcgccaccgc ctgtagtttt attgcccgcg cgttaactcc
    26641 cgcgcgggta attgctccat ccattgcata aacacctgcg gcggcatcgc cttcgcaaaa
    26701 aaccatccct ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg ctcctcagtt
    26761 tcgacgcctt cagcgatcgt ttttaacccc aggctgtgcg ccagctcgat gatgtgttcc
    26821 gcaatcaaat gactggtttt gtgggtggtc agcgtttcaa caaacgattt atcgattttc
    26881 aaaatatcga cattcaatga tttaaggtta tgcaagttag agtagccaat accaaaatca
    26941 tcaattgcca cttcgtaacc agcctggcgg aaagccagaa ttaatcggcg tcattttttc
    27001 gacatcaaga aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta
    27061 ttgctctgtt ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga
    27121 caggttaata gaaacatagc gatcggcatg tgttgccagg tacgcgccca gatcgcggaa
    27181 gacattatca ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg
    27241 aataaactct gccggattca ttatttgccc ctgctcacca ggccaacgta acaacgcttc
    27301 agcgccgata catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg
    27361 atgtttttcg agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg
    27421 tagccagagt aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag
    27481 atgattataa aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg
    27541 gcgttttgtc gaataaattg tcgcatataa atagccattt ctttgtacgg ttaaatcctt
    27601 caaatgaatc agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt
    27661 tttcatcacc gtatcataca caccccattg caatgtcggg tcatcagaca tcacttcact
    27721 ccagaagaga gggttgataa ccgccacata atttccccgc tgcatatagg tcattttata
    27781 gccagagaaa aaaggcgtat cgcggtaata atagatagaa acgttaggtt cacgcttata
    27841 atcggccggt gcaatcgtat agccgtttac aggcgctatc agcgatgagc ataaaaaatg
    27901 gttatcacga attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga
    27961 tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc
    28021 tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta
    28081 ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga
    28141 actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct
    28201 atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaccctggc taccgacgtc
    28261 tttagataaa tcttacggta taaatagcac ttttgcctga tttgaaattg ataccttttt
    28321 aatcgtcacc tggatgaaaa taattcattg atattcaaaa ttaattttct acaggaatac
    28381 agaggcggcg ggaagatatg cccttaatgc cactgaaaaa ccacataatt tgtacagcaa
    28441 taaaagtcac ggcctaatat ggctaacaaa caatcaattg tgatatagtt cacaaaatta
    28501 atgaaacaaa cagagtgttt catttttgtg ttctgcaagc cccgcgatag ccagagtgta
    28561 ccacgcctcc cgtgaacaac gccgcgctgt ccagggcatc ggctcttttc agaggaattg
    28621 tttgatggct accctcacca ctggcgtggt tcttcttcgc tggcaacttc ttagtgccgt
    28681 aatgatgttt ctggccagca cactcaacat ccgttttcgt cggtctgatt atgtcgggct
    28741 tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa tggggttgct
    28801 tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagtcgg tgatgataga
    28861 agagatgaat cagacaccgc cacaatggcc aatgattttg acctgatacg tagaagc
//
LOCUS       DAJTYH010000049        28802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTYH010000049 DAJTYH010000000
VERSION     DAJTYH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..2025)
                     /gene="yccS"
                     /locus_tag="OO609_002085"
     CDS             complement(<1..2025)
                     /gene="yccS"
                     /locus_tag="OO609_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6633498.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQ"
     gene            complement(2035..2481)
                     /locus_tag="OO609_002086"
     CDS             complement(2035..2481)
                     /locus_tag="OO609_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6633499.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2604..4658
                     /gene="helD"
                     /locus_tag="OO609_002087"
     CDS             2604..4658
                     /gene="helD"
                     /locus_tag="OO609_002087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6633500.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4690..5148)
                     /gene="mgsA"
                     /locus_tag="OO609_002088"
     CDS             complement(4690..5148)
                     /gene="mgsA"
                     /locus_tag="OO609_002088"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6633501.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5244..5906)
                     /locus_tag="OO609_002089"
     CDS             complement(5244..5906)
                     /locus_tag="OO609_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6633502.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6079..6492
                     /locus_tag="OO609_002090"
     CDS             6079..6492
                     /locus_tag="OO609_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6633503.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6537..6854)
                     /gene="hspQ"
                     /locus_tag="OO609_002091"
     CDS             complement(6537..6854)
                     /gene="hspQ"
                     /locus_tag="OO609_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6633504.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6912..8102)
                     /gene="rlmI"
                     /locus_tag="OO609_002092"
     CDS             complement(6912..8102)
                     /gene="rlmI"
                     /locus_tag="OO609_002092"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6633505.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8197..8475
                     /gene="yccX"
                     /locus_tag="OO609_002093"
     CDS             8197..8475
                     /gene="yccX"
                     /locus_tag="OO609_002093"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6633506.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8472..8801)
                     /gene="tusE"
                     /locus_tag="OO609_002094"
     CDS             complement(8472..8801)
                     /gene="tusE"
                     /locus_tag="OO609_002094"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6633507.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8892..9551)
                     /gene="yccA"
                     /locus_tag="OO609_002095"
     CDS             complement(8892..9551)
                     /gene="yccA"
                     /locus_tag="OO609_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6633508.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9758..9845)
                     /locus_tag="OO609_002096"
     tRNA            complement(9758..9845)
                     /locus_tag="OO609_002096"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9809..9811),aa:Ser,seq:tga)
     gene            10271..11389
                     /gene="hyaA"
                     /locus_tag="OO609_002097"
     CDS             10271..11389
                     /gene="hyaA"
                     /locus_tag="OO609_002097"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6633509.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11386..13179
                     /gene="hyaB"
                     /locus_tag="OO609_002098"
     CDS             11386..13179
                     /gene="hyaB"
                     /locus_tag="OO609_002098"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6633510.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13198..13905
                     /gene="hyaC"
                     /locus_tag="OO609_002099"
     CDS             13198..13905
                     /gene="hyaC"
                     /locus_tag="OO609_002099"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6633511.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13902..14489
                     /locus_tag="OO609_002100"
     CDS             13902..14489
                     /locus_tag="OO609_002100"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6633512.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14486..14884
                     /gene="hyaE"
                     /locus_tag="OO609_002101"
     CDS             14486..14884
                     /gene="hyaE"
                     /locus_tag="OO609_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6633513.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14881..15738
                     /locus_tag="OO609_002102"
     CDS             14881..15738
                     /locus_tag="OO609_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6633514.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15872..17416
                     /gene="appC"
                     /locus_tag="OO609_002103"
     CDS             15872..17416
                     /gene="appC"
                     /locus_tag="OO609_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6633515.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17428..18564
                     /gene="appB"
                     /locus_tag="OO609_002104"
     CDS             17428..18564
                     /gene="appB"
                     /locus_tag="OO609_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6633516.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18576..18668
                     /gene="cbdX"
                     /locus_tag="OO609_002105"
     CDS             18576..18668
                     /gene="cbdX"
                     /locus_tag="OO609_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6633517.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18748..20046
                     /gene="appA"
                     /locus_tag="OO609_002106"
     CDS             18748..20046
                     /gene="appA"
                     /locus_tag="OO609_002106"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6633518.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20161..22327)
                     /gene="etk"
                     /locus_tag="OO609_002107"
                     /pseudo
     CDS             complement(20161..22327)
                     /gene="etk"
                     /locus_tag="OO609_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22347..22793)
                     /gene="etp"
                     /locus_tag="OO609_002108"
                     /gene_synonym="yccY"
     CDS             complement(22347..22793)
                     /gene="etp"
                     /locus_tag="OO609_002108"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6633519.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22781..23920)
                     /locus_tag="OO609_002109"
     CDS             complement(22781..23920)
                     /locus_tag="OO609_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6633520.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(23966..26062)
                     /locus_tag="OO609_002110"
     CDS             complement(23966..26062)
                     /locus_tag="OO609_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6633521.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26062..26808)
                     /locus_tag="OO609_002111"
     CDS             complement(26062..26808)
                     /locus_tag="OO609_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6633522.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26805..27449)
                     /locus_tag="OO609_002112"
     CDS             complement(26805..27449)
                     /locus_tag="OO609_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6633523.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27556..27861)
                     /locus_tag="OO609_002113"
     CDS             complement(27556..27861)
                     /locus_tag="OO609_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633524.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28303..28515)
                     /gene="cspH"
                     /locus_tag="OO609_002114"
     CDS             complement(28303..28515)
                     /gene="cspH"
                     /locus_tag="OO609_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6633525.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6974 a         7294 c         7576 g         6958 t
ORIGIN      
        1 ctgcatccgc tgtttcaggc cagctaatgc ctgattgacg cgttcttcat cagcaggttg
       61 atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca agaaacgcca gaatttcagg
      121 attagttaac tgctcccggt gagcaccgag ggctgagata tagctggtaa acgtatggtt
      181 aaggcacagc aagcgaaacg cggcttcgcg aatttgcggg gtaacgttcg gttcgctgga
      241 catatttgat accaccgacg ccagttcagc atcacggttg tgtgcatcgc ggcgggcaat
      301 acgatacgcc agacggttat cacgcccctg atggtattgc tccagtatgg catcgagata
      361 gcgacagttg gcctctgtgg cgcgttcgag cattcgcggc agattgcgaa acttccagtc
      421 aggccagatg tagctcactg ccgcccacgc aatggcacaa ccaatcagcg tatcgattac
      481 gcgaggtaac gctacttcaa aaccttcacc cagtaagtta aaacacagta gcaccaaaag
      541 tgtgatgaac atcgttgcat gagcgtattg cacgttacgg aaggcaaaaa agagcacgcc
      601 ggtaataacc agcagcacca gctgcccttc cagtgatggc acaaaccaca gcacaggaat
      661 gccaatcgcg atacctacca gcgtaccaat aatccttaac ttcaggcggt ggcgcgtggc
      721 gttatagttt ggctggcaga caaacaaact tgtcagcaag atccaatacc cgtgatgcat
      781 tccggttatt tgaatgatgg cgtagccgaa gcacaacacc agcgacattc ttaccgcatg
      841 acggaagagg gcggattccg gcgtgaagtg acggctaaga cgcagccaga tatcactcaa
      901 tccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta ttacggggta gtgtctgggc
      961 ctgttctgat tcaattgttg ccagttgggc atcaatggcg cgtaaattgt tcagcaaaaa
     1021 tcccagtgtt ttgagtaaat cggcgggtgc gccgttatcg cgtatccgct ccagcgcagc
     1081 atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt tgataagact gacgcaacaa
     1141 aatacagcgt gacagttgct ggcacgcctg gccctgcatc gacatcagcc gctgaaaacg
     1201 gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa tatgagaaga
     1261 gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa tgcagcgtgc gacgcgttcc
     1321 ccgttgacca cgatcgccac gtaagcgggt cagcagcgag agtttcgtct gattcaatgt
     1381 cgccatcagc tgaccgttgg cgagagccaa atcgtacagc ggtgcctggc tttcatcttc
     1441 aatatcagga tcaaacatgc gcgacttgag ctcaagataa cgcgccagtt gttcatagca
     1501 acgcgccagg ttgtcctgca gcgggcggac cgggaacagc agatgaccaa taagtgtcag
     1561 gacgttgtac cagacggcac cggccagcag atacatcggc tgctgatacc agtgctcata
     1621 cagtgatgtt cccaacatag tgtaaatggc gatcagcaat gcaccgaagg caattgttgc
     1681 atagcgttga cccagaccgc cgagcaaaat gaagccgctg gtagagagcg ttaagccaat
     1741 cgcaaatagc cagggccagg gaaacagcaa ttctactgag gccgaggcga taaaaaagca
     1801 gaacagcgta atgatgaggt tacgcaaacg tcccgccagt cggtcatcga gatcggtcag
     1861 cgccgctgcc accatcccca gcgttagcgg aatcgtcagt tttacatcac ccagccacca
     1921 cggaaacgct gtggttccac aaagcgcaat aaaaatacgc gcgtaataca gccaggcgct
     1981 gttccaggta tagcgtttga gcaaaggact tagcataaag gccatattag tggattattc
     2041 aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg ataccacgcg
     2101 acgaccaacc ggccatagtg caatagcggc aattttaaag ttcgcaatgc cgacaggaat
     2161 gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc ataaccacca
     2221 gccaaagaaa atcagccaga aaatattcaa taccgtaccg ccagtattca gcaacacatt
     2281 tttgccagcc gggttcagtt catcgacatg aatagcttca ttgccataag gcaccagaga
     2341 cagtttagtg atctcccagc aggatcgtgt cagcggtaag gtaaaaatca gcacaatact
     2401 gaccagagtc gccaacagcc agcccagagt ggtggcaaat ccgccaagca caaagttcag
     2461 aatgttcaaa acggtacgca taaaacctcg ctttactgtg gttttcagta atgggcggca
     2521 attgtaacgt ttttttgggc tggagcacgt tttctctgac ggttacactg ataagaaata
     2581 atttcgtgtg gatctacaga gtcatggaac tgaaagcgac aacgcttgga aaacgtctgg
     2641 cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc tccggcgatc
     2701 gccacgaata ccttattcct ttcaatcaat tactggcgat tcactgtaag cgcggtctgg
     2761 tatggggcga gctggaattt gtactgccgg acgaaaaagt ggtgcgtctg cacggcacag
     2821 aatggggcga gacgcagcgt ttttaccatc atcttgatgc tcactggcgg cggtggagtg
     2881 gcgagatgag cgaaattgcg tctggtgttt tacgccagca actggatttg attgccacgc
     2941 gcactggaga aaataaatgg ctgacgcgtg agcaaacctc tggcgttcag caacaaatcc
     3001 gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc gataactgcc
     3061 gtgaggcgtg gcgtaaatgt caggcttggt tgaaagatat tgaaagcgct cggttgcagc
     3121 ataaccaggc gtataccgaa gccatgctta ccgagtatgc ggattttttc cgccaggtcg
     3181 agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt taatggcgag cattctctgt
     3241 tagtgctggc tggtgcagga agcggaaaaa cgtcggtgct ggtggcccgt gcaggctggt
     3301 tgctggcgcg tggtgaagcg tcccctgagc aaattttatt gctggcgttt ggtcgcaaag
     3361 ccgctgaaga gatggacgag cggattcgcg aacggctaca taccgaagac attaccgcac
     3421 gcacgtttca tgcgttggcg ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag
     3481 tcagcaaact ggaaaatgat accgctgccc gtcatgaact ctttattgct gagtggcgca
     3541 agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg acggaagaaa
     3601 tgcagtggtc agtgccagaa ggtaacttct gggatgatga aaaattacag cgtcgccttg
     3661 cctctcgcct cgatcgctgg gtaagtctga tgcggatgca cggtggtgca caggcagaaa
     3721 tgattgccag tgcacccgaa gagattcgcg atctgttcag taaacgtatc aagttgatgg
     3781 ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg
     3841 gccttattca tcaggcgatt gtgattctgg agaaaggccg ctttatcagc ccgtggaagc
     3901 atattctggt tgatgaattt caggatatct cgccgcagcg cgcagcgttg ttagcggcat
     3961 tacgcaagca aaacagtcag acgacgttgt tcgccgttgg tgatgactgg caggcgattt
     4021 accgattcag cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag
     4081 gcgatcgctg tgatttagac acgacttacc gttttaacag tcgtatcggt gaggtggcaa
     4141 accggtttat tcagcagaac ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg
     4201 gagacaaaaa agccgtcacg ttattggatg agagtcaact tgacgctttg ctggataagc
     4261 tctctggtta tgccaaaccg gaagagcgca ttctgatcct ggcgcgttac catcacatga
     4321 ggcctgccag cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc gactttatga
     4381 ccattcatgc cagcaaaggg caacaggcgg attacgtcat catcgttggc ttgcaggagg
     4441 ggagtggtgg ttttccggct gcggcgcggg agtcgattat ggaagaggcg ctactgccac
     4501 cggttgagga tttcccggac gctgaagaac ggcgtttaat gtacgtggcg ctgacccggg
     4561 cacgccatcg ggtatgggca ctgtttaaca aagagaatcc ctctcccttt gtggaaatac
     4621 tgaaaaatct ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc
     4681 tgtgcaatat tacttcagac ggtcagcgag ataacgctga taatcgggga taagaatatc
     4741 gaccgcgtcg ttgaaatgcg gcgactggat aatgaagtct gccgttgcca cgttggtggc
     4801 gaccggaatg ttccataccg tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg
     4861 cacggcattt agtggatccc agaagaatat caatacatca attttccctt ctgagatcaa
     4921 tgcgccaacc tgctggtcac cccccattgg gccactcaac atcgcgttga cgttcatgcc
     4981 ggtcgcgcgg gaaatcaagt taccggtagt gcctgttgca tacagtacgt gttgttccag
     5041 taacggttga tgccgttcca cccagctcat cagcatttgt ttgcagtgat cgtgtgccac
     5101 cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc agttccataa tgtacatccg
     5161 tagttaactt tcctacagat tactgtaagc acttatcgct gcaagataaa gaccgaaaaa
     5221 gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt tttccgccca ttgcaggaaa
     5281 gtatttcgcg tttgtgggtc agccagttta aaccaatact tcagccgttg ttctgtgagc
     5341 acctgagact gcggtggaat agcatccagc tcggaaacac ccgaaagcag cgtactgtca
     5401 tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt
     5461 tcaacctcga cctcataatc aatcgttttt gccgttgagg taatggcgag aatatccagc
     5521 tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc ggcatcaaaa
     5581 tggttagctt cccgctcatt ctccaggcga ggcaggcgaa aattgacctg gttgatgagc
     5641 tgggtattaa aactgaccac cagcggtggg gagatataca gccgttcttc actattggaa
     5701 agatgaatcg tcttctcaac gcgaaacact aactgatgcg gtccattgtc cagttcaatg
     5761 ctgtccgcac cgcgcagcag agagctggaa accttcttgc cgtcgagtac cagaagatcg
     5821 acatcggttg aaagccgcag cgtggtggca aaaacggata ccggcaaaca taatgcaatc
     5881 aaggtggtca cgatgccggt tttcataggt agctcctgtc aaaagaccgc ccgaataata
     5941 atgtgtcaaa atttttcttt aatcatactt actaacatat agacatattt cccgcttttg
     6001 ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta cactttgtta
     6061 aacagtctgg aggaaataat gaaagaaacc gatattgcag gcattttaac gtccactcac
     6121 accattgcgc tggtgggggc aagcgacaaa cccgatcgcc ccagctatcg cgtgatgaaa
     6181 tatctactcg accaggggta tcacgtcatt ccggtttcgc caaaagttgc cggcaaaacg
     6241 ctgctgggcc agcaggggta tggcacgctg gcagacgttc cggaaaaggt cgatatggtc
     6301 gatgttttcc gtaattcaga agcggcgtgg ggcgtggcac aggaagctat tgccattggc
     6361 gcgaaaaccc tctggatgca actgggggtg attaatgagc aagcggcagt actggcacgg
     6421 gatgccgggt taaatgtggt gatggatcgc tgcccggcta ttgagatccc tcgcctgggt
     6481 ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg gggatttgtt cagggattag
     6541 ttacgcagac gcggggcctg gagttgtttg cggatggtct gcgccagctc atccatggat
     6601 ggctgttcag gatgttcatc ctgcaattcg ctacttagct gggcttcagc caggtaggta
     6661 tgaaccggta ggccgttatc gtcctccatc accacgtgat accacggagc ggcgcgaagc
     6721 tcgtcattca ccgccagctc atcgggcgac ggttcagaga gcgaataaac cgggtcgata
     6781 tccacgacca ctccgagata acctaacagg gaatggcgga cctgctggcc gataccgaat
     6841 ttgctggcaa tcatagtcac ctcccgggaa atctttacct ttgatataag ggtaaaattc
     6901 cacatttcaa gttacatgac gcgacaggca aaccctttca gatatagccc ttccgggtag
     6961 gtagcgatca ccggatgatc ggctgcctga cggaactgct ctataaattg tacatcacga
     7021 ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt catcagaccg
     7081 gaacaggaga aagtcaggag aataccgcct tcattcagca actgaatcgc cagcatgttg
     7141 atgtctttat aaccacgaca cgcgcccatc aactggcttt tattctcaac aaacttcggc
     7201 gggtccatca cgataacgtc aaatttttca ccgcgatcgc gataagtacg cagcaattta
     7261 aagacatcat cacggacaaa ctcagccttg ctcagatcca gtttgttcag ctcaacgttc
     7321 tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg
     7381 cccatcagtg ccgatacggc gaaaccaccg gtataggaga aacagttcag cacacgttta
     7441 ttttcaacgt agcggcgggt agccaggcgg ctgtctcgct ggtccagata gtagcccgtt
     7501 ttgtgtccgt gctgaatatc caccagcagc ttcatgccgt gttcttcaat cggcagcagg
     7561 gcaggcggca actcgccggt gacgaggccc tgggtcagct ccatcccttc ttttttacgt
     7621 accgcaacat cgctgcgatc gtaaatcgca cattccgggt acagcgtttg cagggcacta
     7681 attaatgccg cgcgctgata ttctgcgcca gcactcagca gttgcagcac cagaaaatta
     7741 ccgaaacgat cgatagtaat acccggcagg ccatcagatt ctccggcgat taaacgatag
     7801 ctgtcgaggc catctttttg cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg
     7861 cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa acgtccagac gcgcgcccgg
     7921 atttgcgaag ctggcgaata agcgccgcgt gctaaccatt ttccctgatg atcaacaata
     7981 tcgatggttt caccgaggct ggctttacct tccatgcggg caacggcccc ggaaaagacc
     8041 cacggatggc gacgaagtaa tgatttttcg cgccctttgg ctaacactaa acgtacactc
     8101 ataatttact tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga
     8161 tttgcaacgc gtccattaag gataaggaga gcagcaatgt cgaaaatctg cataattgcc
     8221 tggatttacg ggcgagttca gggcgtagga tttcgctaca ccacacagta cgaagcgaaa
     8281 agactgggct taaccgggta cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc
     8341 tgcggtgaag aagggcaggt agaaaaatta atgcagtggt taaagagtgg cggcccgcgt
     8401 tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc cctcggggga attaaccgat
     8461 tttcgtatcc gttaaataca ttttaccggt tttggcaggc cagcaatttt ggtggcttgc
     8521 ttagccgggc ctttcgggaa cagtcggtat aaatagcggc tgttaccttt ttcttcacca
     8581 aatttattcg ccatcgcttt taccagcata cgaatcgccg gagacgtatt aaattccagg
     8641 tagaagtcac gtacaaaacg caccacttcc cagtgttctg gcgacagcga aatcccttcg
     8701 ttctctgcaa tcaccactgc cagcggttca ctccactggc tgctttcttt gagatagcct
     8761 tcggtatccg tttctatctc tttaccttcg aagatcagca taattattac taccttaatc
     8821 agactggcgg cagtgtaaca aaaaacaaag ccccgcataa agcgaggcta tgaaagtgtt
     8881 agcgggtgag attaatcgcg gctggcgaag cccagaatgc tcagtaggct gacgaagatg
     8941 ttgtacagcg aaacatacag gctaaccgtg gcacgaatat agttcgtttc accgccatga
     9001 atgatgttgc tggtttcaaa caagatagcg ccagaggaga tcagaatgaa gaccgcgctg
     9061 atcgccagat gcagagcagg cagttgcagg aagatattag caatcatacc aatcagtacc
     9121 accacaatac ccgccatcag cataccgccg aggaacgaca tatctttgcg ggtggtcagc
     9181 acatatgcag agcagcagaa gaacactaac gccgttccgc ccagtgccat agcgattacg
     9241 tcacccattc cggcagacag ataggtgttc agaattggtc cgaggatata acccagaaaa
     9301 ccggtaaagg cgaatgcgga gataatcccg gtcggcttat tcgccgtttt ataggtcagg
     9361 aacatcaaac catacatacc caccagagtc agaatcagac ccggagatgg cagcatcagc
     9421 accgtactgg cagttgcggt aatcgccgaa aaggccagcg tcaggctcag cagaaaatag
     9481 gtattacgca gcaccttatg ggtgctaagc agtgatgtac ggtcatgtga agaactaaca
     9541 atacgatcca ttgagtcact ctctatgaca gatgtaatta attaagcagc ataatgataa
     9601 tgcgtaaggg cacccagaag ttttacccat ctttacgcat ttgatctgga acaggtttaa
     9661 cagcggatta tcaggtcatt aagcaaatat aacgccctga gaattccgac aggcaaaaga
     9721 aaaagggggt agcgtttagc taacccctta tcttatttgg cggaagcgca gagattcgaa
     9781 ctctggaacc ctttcgggtc gccggttttc aagaccggtg ccttcaaccg ctcggccaca
     9841 cttccggaat gacgcgcact ataaacatcc cgatgcggcg tgtaaacccc taatttgttt
     9901 gtttgcctga aaaacagcca aaagtgcatt gatagcgtga aataacagca gattgatcat
     9961 ttcatcacca tgaattcctt ctcttttact cgtttagcaa ccggctaaac atccccatcg
    10021 cccggctaaa gaaaaatagg tccattttta tcgctaaaag ataaatccac acagtttgta
    10081 ttgttttgtg caaaagtttc actacgcttt attaacaata ctttctggcg acgtgtgcca
    10141 gtgcagaagg atgagctttc gttttcagca tctcacgtga agcgatggtt tgccttgcta
    10201 cagggacgtc gcttgccgac cataagcgcc cggtgtcctg ccggtgttgc taggagaaga
    10261 gacgtgcgat atgaataacg aggaaacatt ttaccaggcc atgcggcgtc agggcgttac
    10321 ccggcgcagc tttctcaaat attgtagtct ggctgccacg tcgctgggat taggcgcggg
    10381 aatggcacca aagattgcct gggcgctgga gaacaaaccg cgcattccgg tggtatggat
    10441 ccacggtctg gaatgtacct gctgtaccga atcttttatc cgctccgctc acccactggc
    10501 gaaggacgtc atcctttccc tgatttccct cgattacgac gataccctga tggctgccgc
    10561 cggaacccag gcggaagaag tctttgaaga catcatcacg caatataatg gcaaatatat
    10621 cctcgcagta gaaggtaatc cgccgctggg cgagcagggg atgttctgta tcagcagcgg
    10681 tcgaccgttt attgagaaac tcaaacgtgc cgctgccgga gccagcgcga ttatcgcctg
    10741 gggaacctgc gcgtcctggg gctgcgtgca ggccgcgcga cctaatccga cacaggcaac
    10801 gcctatcgac aaagtcatca ccgacaaacc cattatcaaa gtacctggct gcccgccgat
    10861 cccggatgtg atgagcgcca tcattactta catggtgacc tttgatcgct tgccagatgt
    10921 cgacagaatg ggtcgtccac tgatgtttta tggtcagcgt atccacgata aatgctatcg
    10981 ccgcgctcac ttcgacgccg gagagttcgt ccagagttgg gatgatgacg ctgcccgcaa
    11041 aggttattgc ctgtacaaaa tgggctgcaa agggcctacc acctataacg cctgttcctc
    11101 cacacgctgg aatgatggcg tttctttccc aatccagtct ggtcacggct gcctgggctg
    11161 tgcggaaaat ggtttctggg atcgcggttc gttctacagc cgcgtggtcg atattccgca
    11221 aatgggtact cattccaccg ccgataccgt cggtttaacc gcgcttggcg tggtggcagc
    11281 agctgttggt gtgcacgcag ttgccagcgc cgttgaccaa cgcagacgtc ataaccagca
    11341 acctacagaa accgaacatc agccaggcaa tgaggataaa caggcatgag cactcagtac
    11401 gaaactcagg gatacaccat caataatgcc ggacgccgcc tggtggtcga cccgattacg
    11461 cgcatcgaag gccacatgcg ctgtgaagtg aatattaacg atcagaatgt gatcaccaat
    11521 gccgtctcct gcggcaccat gtttcgcggg ctggagatca tcctgcaagg gcgcgacccg
    11581 cgcgatgcgt gggcgttcgt tgaacgtatc tgcggcgtct gtactggcgt acacgccctg
    11641 gcttcggttt acgccatcga agatgccatc ggtattaaag tgccggacaa cgccaatatc
    11701 atccgcaaca ttatgctggc aacgctctgg tgccacgatc atctggtgca cttctatcag
    11761 cttgccggga tggactggat cgacgtgtta gatgcgctga aagccgaccc gcgaaaaacc
    11821 tccgaactgg cgcaaagtct ctcctcttgg ccgaaatcat cccctggcta tttcttcgac
    11881 gtacaaaatc gcctgaagaa attcgttgaa ggcgggcagt tggggatctt ccgcaatggc
    11941 tactgggggc acccgcagta caaactgccg ccagaagcca acctgatggg ctttgcccac
    12001 tatctcgaag ctctcgattt ccaacgtgaa attgtcaaaa ttcacgcggt ctttggcggt
    12061 aaaaacccgc atccaaactg gattgtcggt gggatgcctt gcgccattaa cattgacgaa
    12121 agcggcgcgg tcggggcggt caatatggaa cgcctgaacc tggtgcagtc gattatcacc
    12181 cgcacggcag acttcattaa caacgtgatg atccccgacg ccttagccat cggtcagttc
    12241 aacaaaccgt ggagcgaaat cggcacgggt ctttctgata aatgtgtcct cagctacggc
    12301 gcattcccgg atattgccaa cgactttggt gagaaaagtc tgctgatgcc tggcggcgcg
    12361 gtgattaacg gcgacttcaa caatgtgctg ccagtggatt tggttgatcc gcagcaggtg
    12421 caggagtttg tcgatcacgc ctggtatcgt tatcccaacg atcaggtcgg gcgtcatcca
    12481 ttcgatggca ttaccgaccc gtggtacaac cccggcgatg tcaaaggcag cgataccaac
    12541 attcagcagc tgaatgaaca ggaacgctac tcgtggatca aagcgccacg ctggcgcggt
    12601 aacgcgatgg aagtggggcc gctggcacgc acgttaatcg cttatcacaa aggcgatgct
    12661 gcgaccgttg agtcggtcga tcgcatgatg tcggcgttga acctgccgct ttccggtatc
    12721 cagtcaacgt tgggccgcat tttgtgccgc gcgcacgaag cgcagtgggc cgcaggtaag
    12781 ttgcagtatt tcttcgacaa gctgatgacc aacctgaaaa acggcaatct cgccactgct
    12841 tccactgaaa aatgggaacc tgcaacctgg ccgacagagt gccgtggcgt cggctttacc
    12901 gaagcgccgc gcggagcgtt aggccactgg gccgccattc gcgatggcaa gattgatctc
    12961 taccagtgcg tggtgccgac cacctggaac gccagtccgc gcgatcccaa agggcagatt
    13021 ggcgcttatg aagcggcgct gatgaacacc aaaatggcga tccccgagca accgctggag
    13081 atactgcgta ctctgcacag ctttgatccg tgcctcgcct gttcaactca tgtgctgggc
    13141 gacgacggta gcgagctgat ctccgtgcag gtgcgttaac agcgaaggag aatcatcatg
    13201 caacagaaaa gcgacaacgt tgtcagccac tatgtctttg aagcaccagt tcgtatctgg
    13261 cactggctga cggtgttatg catggcggtg ttgatggtca ccggttactt tatcggcaag
    13321 ccgctacctt ccgtcagtgg cgaggcgacg tatctgttct atatgggtta catcaggtta
    13381 attcacttca gcgccgggat gatttttacc gtggtcttgc tgatgcggat ctactgggct
    13441 tttgttggca atcgttactc ccgcgagctg tttatcgtgc cggtatggcg taaaagctgg
    13501 tggcagggcg tgtggtatga aatccgctgg tatctgtttc tggcaaaacg tccgagtgcc
    13561 gatataggcc ataaccccat cgcccaggcg gcgatgttcg gctatttcct gatgtcggtc
    13621 tttatgatca tcaccggttt tgcgctgtac agcgaacaca gccagtacgc tatttttgcg
    13681 ccgttccgtt atgtggtgga atttttctac tggacgggtg gcaactcaat ggacattcac
    13741 agctggcatc ggctggggat gtggctgatt ggggcgtttg tgatcggtca tgtctacatg
    13801 gcgctgcgtg aagacatcat gtccgacgac acggtgatct ccaccatggt caacggctac
    13861 cgtagccaca aatttggcaa aataagtaac aaggagcgtt catgagcgag caacacgtgg
    13921 tggtcatggg gctgggcaac ctgctgtggg ccgatgaagg cttcggcgtg cgggtggcgg
    13981 aacggctgta tgcccattac cactggcccg agtatgtgga gattgtcgat ggcggtactc
    14041 agggactgaa cttgctgggg tatgtcgaaa gcgccagcca tctgttgatt ctcgatgcca
    14101 ttgactacgg gctggaacct ggaacgctgc gaacctatgc cggagaacgc attccggcct
    14161 atctcagcgc gaagaaaatg agcctgcatc agaacagttt ctccgaagtg ctggcgctgg
    14221 cagatatccg cggacatctg ccagcacata ttgccctcgt cgggctgcaa cccgcgatgc
    14281 tcgacgacta cggcggcagc ctgagcgaac tggcacggga gcaactgccc gctgcggaac
    14341 aatcggcgct ggcgcagttg gctgcgtggg gaagtgtgcc gcaaccggct aatgaatcgc
    14401 gttgtctcaa ttatgactgt ctgtcgatgg aaaattacga aggcgttcgc ttgcgccagt
    14461 accggatgac acaggaggag cagggatgag caacgacacg ccatttgatg cgttgtggca
    14521 acgaatgctg gcgcgcggct ggacgccggt cagtgaatcc cgtcttgacg actggcttac
    14581 gcaagcgcca gacggcgtgg tgttattaag cagtgacccg aaacgcacgc cagaggtcag
    14641 cgataatccg gtaatgattg gcgaattact gcacgagttt cccgactata catggcaggt
    14701 ggcgattgct gaccttgagc agagcgaagc catcggcgat cgctttggcg cctttcgctt
    14761 tcctgccact ttagtgttta ccggcggaaa ctatcgcggc gtgctgaatg gtattcaccc
    14821 gtgggcggaa ctgataaacc tgatgcgcgg gcttgtcgaa ccgcagcagg agcgtgcctc
    14881 atgagcgaaa cttttttcca tctgctgggg ccaggaacgc aaccgaacga tgacagtttc
    14941 agcatgaatc cactgccgat cacctgtcag gtgaatgatg aaccgagtat ggcggccttg
    15001 gagcaatgtg ctcacagccc gcaggtgatt gcgctgttaa acgagttaca acatcaacta
    15061 agcgaacgcc agccgccgtt gggcgaggtg ctggcagtcg atctgttaaa tctcaacgcc
    15121 gacgatcgtc actttatcaa tacgcttctc ggggaagggg aagtgtcagt gcgcattcag
    15181 caggctgacg acagtaaaag tgaaatacag gaggcgatct tctgcggatt atggcgggtg
    15241 cgcagacgtc gcggcgaaaa gttgctggag gacaaactgg aggctggctg cgcgccgctg
    15301 gcgttgtggc aggcggcaac gcaaaatctc ttgccgacag attcgctgtt accgccgccc
    15361 attgatggcc tgatgaatgg cctaccgttg gcgcatgagt tactggcaca tgtacgtaac
    15421 cccgacgcgc agccgcacag cattaatctg acgcaattac ccatcagcga ggctgatcgg
    15481 ctttttctct cgcgtctctg tgggccggga aatattcaga ttcgtaccat tggctatggc
    15541 gagagctata tcaacgccac ggggttacgc catgtctggc atttacgctg tacggacacc
    15601 ttaaaaggcc cgttactgga aagttatgaa atctgcccaa taccggaagt ggtgctggca
    15661 gcgccagaag atttggtcga ctctgcgcag cggcttagcg aggtatgtca gtggctggcg
    15721 gaagctgcac cgacgtaaaa agacggtaag tatcgctttc agtcttatga atatcgcaat
    15781 cggcgaatac ctctggtcgt agagtttcag gataaagagg gagatctaac attatcgggt
    15841 tatttttctc tcttcgccta caggagtgcg catgtgggat gtcattgatt tatcgcgctg
    15901 gcagtttgct ctgaccgcgc tgtatcactt tttatttgta ccccttaccc tggggctgat
    15961 ttttttgctg gctatcatgg aaaccattta cgtggttacc ggcaaaacaa tctaccgcga
    16021 tatgacgcgc ttctggggta agctcttcgg tatcaatttt gctcttggcg tggctaccgg
    16081 cctgaccatg gagtttcagt ttggtactaa ctggtcattc tattccaact atgtgggcga
    16141 tatttttggc gcaccactgg cgatggaagc attaatggcc ttcttcctcg aatccacctt
    16201 tgtcgggctg ttcttcttcg gctggcaacg gctgaataaa taccagcacc tgctggtgac
    16261 gtggctggtg gcgttcggtt caaatctctc tgcattgtgg atattgaatg ccaacggttg
    16321 gatgcaatac ccgaccggtg cgcattttga tatcgacacc ctgcgtatgg agatgaccag
    16381 cttcagcgag ctggtcttta acccggtcag ccaggtgaaa tttgtgcaca ccgtcatggc
    16441 gggctacgtg accggggcca tgtttattat ggcgatcagc gcctggtatt tactgcgcgg
    16501 acgggagcgc gatgtcgcat tacgctcgtt tgccatcggt tccgtcttcg gtactctggc
    16561 gattatcggt accctgcaac tcggagacag ttctgcgtat gaagtcgcgc aagtacaacc
    16621 ggtaaaactg gcggcgatgg aaggggagtg gcaaacggaa cctgctcctg caccgttcca
    16681 tgtggttgcc tggccggaac aggatcaaga gcgtaacgcc tttgccatca aaattcccgc
    16741 gctgctaggg atcctcgcca ctcactcatt agataaaccc gtgccgggtc tgaagaattt
    16801 gatggctgaa acctacccac gcttgcaacg cggacgtatg gcctggctgt taatgcagga
    16861 aatatctcaa ggcaatcgtg agccgcatgt gttgcaggca ttccgggagc tggaaggcga
    16921 ccttggttac ggcatgttgc tctcccgcta tgcgccggat atgaatcatg tcacagccgc
    16981 acagtaccag gcggcgatgc gtggcgcgat acctcaggtt gcgccggtat tctggagttt
    17041 ccgcatcatg gtgggctgtg gttccctgct gctactggtg atgctgattg cgcttgtcca
    17101 gacgctgcgt ggcaaaatcg accagcatcg ctgggtgctg aaaatggcgc tctggagttt
    17161 gccgttgccg tggattgcga ttgaagccgg gtggtttatg accgagtttg gtcgtcagcc
    17221 gtgggcgata caggacatct taccgacata ctccgcgcac tccgctttaa ccacaggaca
    17281 actggctttc tcactgatca tgatcgtagg gctttacacc ctgttcttaa tcgccgaagt
    17341 ctacctgatg cagaaatatg cccgtctggg gccgagcgcg atgcagagtg aacaaccgac
    17401 gcagcaacag gggtaaagga gaaaatcatg tttgattatg aaacattgcg cttcatctgg
    17461 tggctgctga ttggcgtgat cctggtggtc tttatgatct ccgacggatt tgacatgggg
    17521 atcggctgtt tgctgccgct ggtggcgcgt aatgatgatg aacgccggat agtgattaat
    17581 agcgttggtg cgcactggga aggcaatcag gtctggttga tcctcgcagg tggggcatta
    17641 tttgcagcct ggcccagagt gtatgcagcg gcgttttccg gcttttatgt ggcgatgatc
    17701 ctggtgctgt gctcgctgtt cttccgtccg ctggcctttg attatcgcgg aaaaattgcc
    17761 gatgcacgct ggcgtaaaat gtgggatgcc ggtctggtca tcggcagtct ggtgccgccg
    17821 gtagtcttcg gtatcgcctt cggcaacttg ttgctcggcg tgccgtttgc cttcacaccg
    17881 caattacgcg tggagtatct cggcagcttc tggcaactgc tgacgccatt ccctttattg
    17941 tgtggtttgc tcagccttgg gatggtgatt ttgcaaggtg gcgtctggtt acaactgaaa
    18001 actgttggtg tgattcatct gcgttcacag ctggcgacta aacgcgctgc actgttggtg
    18061 atgctgtgct ttttgctggc aggttactgg ctgtgggtcg gtattgatgg ctttgtactg
    18121 ctcgcccagg atgctaacgg tccttcccat ccgttaatga aactggtggc agtgctacct
    18181 ggtgcctgga tgaataattt cgtcgaatcg cccgttttgt ggatcttccc gctgctgggg
    18241 ttcttctgcc cattgctgac ggtgatggcg atttatcgtg gtcgcccggg ttggggattt
    18301 ttgatggcat cattgatgca atttggcgtg attttcacgg caggcatcac gctgttcccc
    18361 tttgtcatgc cgtcaagtgt gagtccaatc tccagcctga cgttgtggga cagcacttcc
    18421 agtcagctga cgctgagcat tatgttggta atcgtgctga tatttttgcc cattgtgttg
    18481 ctctacactc tctggagcta ctacaaaatg tggggacgca tgacaacaga aactctccgc
    18541 cgtaacaaaa acgagttgta ctaaggagca ggaacatgtg gtatttactt tggttcgtcg
    18601 gcattttgtt gatgtgttcg ctctccaccc ttgtgttggt atggctggac ccgcgtctga
    18661 aaagttaacg aacgtaggcc tgatgcggcg cattagcatc gcatcaggca atcaataatg
    18721 ttagatatga aaagcggaaa catatcgatg aaagcgatct taatcccatt tttatctctt
    18781 ctgattccgt taaccccgca atctgcattc gctcagagtg agccggagct gaagctggaa
    18841 agtatggtga ttgtcagtcg tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg
    18901 caggatgtca ccccagacgc atggccaacc tggccggtaa aactgggttg gctgacaccg
    18961 cgcggtggtg agctaatcgc ctatctcgga cattaccaac gccagcgtct ggtagccgac
    19021 ggattgctgg cgaaaaaggg ctgcccgcag tctgctcagg tcgcgattat tgctgatgtc
    19081 gacgagcgta cccgtaaaac aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca
    19141 ataaccgtac atacccaggc agatacgtcc agtcccgatc cgttatttaa tcctctaaaa
    19201 actggtgttt gccaactgga taacgcgaac gtgactgacg cgatcctctg cagggcagga
    19261 gggtcaattg ctgactttac cgggcatcgg caaacggtgt ttcgcgaact ggaacgggtg
    19321 cttaatttcc cgcaatcaaa cttgtgcctt aaccgtgaga aacaggacga aagctgttca
    19381 ttaacgcagg cattaccatc ggaactcaag gtgagcgccg acaatgtctc attaaccggt
    19441 gcggtaagcc tcgcatcaat gctgacggag atatttctcc tgcaacaggc acaaggaatg
    19501 ccggagccgg ggtggggaag gatcaccgat tcacaccagt ggaacacctt gctaagtttg
    19561 cataacgcgc aattttattt actacaacgc acgccggagg ttgcccgcag tcgcgccacc
    19621 ccgttattgg atttgatcat ggcagcgttg acgccccatc caccgcaaaa acaggcgtat
    19681 ggtgtgacat tacccacttc agtgctgttt attgccggac acgatactaa tctggcaaat
    19741 ctcggcggcg cactggagct caactggacg cttccaggtc agccggataa cacgccgcca
    19801 ggtggtgaac tggtgtttga acgctggcgt cggctaagcg ataacagcca gtggattcag
    19861 gtttcgctgg tcttccagac tttacagcag atgcgtgata aaacgccgct gtcattaaat
    19921 acgccgcccg gagaggtgaa actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc
    19981 atgtgttcgt tggcaggttt tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt
    20041 ttgtaatgca taaaaaagag cattcagtta cctgaatgct ctgaggctga tgacaaacga
    20101 agaactgtct aatgcgtaga ccggaaaagg cgttcacgcc gcatccggtc actttcagtt
    20161 ttactctttc tcggagtaac tataaccgta atagttatag ccgtaactgt aagcggtgct
    20221 ggcgcgttta atcacaccat tgaggattgc gcctttaata ttaacgccag cctgttccag
    20281 acgctgcatt gacaagctca cctctttggc ggtgttcaag ccaaaacgcg caaccagcag
    20341 gctggtgccg acagagcgtc ccacgaccgc agcattgctc accgccagca tcggcggcgt
    20401 atcgacaatc accagatcgt aatggtcgtt cgcccattcc agtaattgac gcatccgatc
    20461 gcgcatcagc agttcagacg ggttaggtgg cacctgaccg cgagtaatca catcaaagtc
    20521 tcctttgcca aaatgctgga tcactttgtt gagctcatct ttacctgcca gatattccga
    20581 caagccatgt tcattactca cggtaaacag gttatgcgaa taaccacggc gtaagtcggc
    20641 atcaataaat aacacttttt gatcggactg ggcgatcacc gctgccagag ttgaactgac
    20701 aaacgtttta ccactgtctg gcgtcgcacc ggtgatcatc agaatgttat tctcagtctc
    20761 catcatagcg aaatgcagac tggttcgtag cgcacgtacg gcttccacag cagaatccgc
    20821 cgggttatcc accgccagga aggggatatt tttagtacga tggcgctgct gattagaaaa
    20881 taaatttttc ttacgcagac gggtgcgttt atccagccac tcggacattg ggatagtgga
    20941 ataaacgctg atgccgtgct cttccagttg ttccggggct tctacaccac gacgcaacat
    21001 cgcacgcgcc agcacggcac tcaccgaaat aaacaggcca agaataaaac caagcaccac
    21061 attcaacgct tttttcggtt tcactggctg cggctgagtg actgccgggt cgataatccg
    21121 cacgttacca atggcactgg atttcgaaat actcaactcc tgctggcggt taagtaattg
    21181 cagatatacc gcacggcccg cttctacgtc acgacttaaa cgcaacactt cctgttgggt
    21241 ggaaggcatt gccgataccc gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg
    21301 tttttccagc agcgcacgat aagttgggtg atctttctta tacagctggg agatctctgc
    21361 ctcgcggaag gtcagctcat tgagttgatt atcaacgttc acaatctgct caagaacggc
    21421 tttggcttcc aggttaaggt caaccgaatc gcgctgctgg cgataaacgt tgagtttttc
    21481 ttccgcctgg tccagctcgc tgcgcacttc aggtaactgg cgctgtaaga attcaaggct
    21541 ttgtgaatcc tgcgccgcct ggcgagcgat attctgttgc aaatagttgt tagcgatgct
    21601 gttcagaata cgagtaatca actggggatc atcaccagtc atggtaagtt ccagcatccc
    21661 gctttcttta ctgcgttcgc taacggtaaa ggtttcctgc aatgcgttaa tcgcttccag
    21721 ttcggtacgc tggctcagga caaactgtgt tcctggtttg gccttaatgt ccgcgatagt
    21781 cagcgcaacg ccatcttttt ccagacgctg gccgaccata ccattgacgg tgaactcttc
    21841 accttccagt gtatagtggc cgttttcccc aaccgtgagt gtcagttgct gatcctgacc
    21901 attcagttgt ggaatatgca tccagctgat cgccagctca cctagttttt ctttggttaa
    21961 tctcgcccag ccgcgaccca caatcggaaa atacttctgc tcaactatgt cgcgcagatt
    22021 cagttcagca atggttttac cgagaatcat gcgcgattgc agcagttgga tctccggtgc
    22081 agactcgggc gatgagttag ggatcatatc gctcaggccg ctgagaatgg cgttgccctg
    22141 tttttgctca acctggacca gagtatctgc ctgataaatt ggtgtgctta acagcgatca
    22201 gcgtgaataa cgcggtcacg ctgataataa acttacggtg atcccataac tcgccgacca
    22261 gacgaagcag atcgatctca ttttcctgag tgctgcctgg tggcgtattc atatttttag
    22321 ttgtcatcgt attttataaa ctcaaattac cggctgaggc gcttcgccca ttcctgactg
    22381 gcgcgctcca acatactgta gacatgttca aatgcgtcct gacttttacg atagggatcc
    22441 gggatctctt tctgttccag ccattgccca aacagcattg tcttcccgcg gacctcggga
    22501 gcaattgcgg taacctgagc aatatgttcc gactccattg ccagaatcag atcgtaattt
    22561 cgtgccatct cagcagtgag cttacgtccg gcatgtcctt caagagatac gccatgatta
    22621 gcggcaacat ctgccgctgt cgcatctgca gggtgtttta ccagaccatg aacacctgcc
    22681 gatttgactt ttacgcccgg cagacgttta cgcagcaagc gctcgccaat gggcgaacgg
    22741 caaatgttgc cggtacaaac cactaggatt gagttaaatt ttagttgggc catgtttgaa
    22801 tccaccgtga agtctctgtc aggtcatgca ccccggaaat cgtcggtacc agcagagaaa
    22861 tcacacggtt ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg taaggttcaa
    22921 gctggaattc cgttccgagt atcattgccg atgcgtcctt cgcattcagc tgataaatat
    22981 tggcgatttt gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat
    23041 cagcgacatc ctgattcata ccctcagcgt tacccagcgc ttctgccagg gtcataccgc
    23101 tgcggtccat tttcagcgtg ctctgtttac ccacttcacc catcacgaag actttcagat
    23161 catcgttgcg cggaataaat aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt
    23221 cgccacgctg catcagcgca tagaggttca ctttggtctt tacgccattc tgggtgagca
    23281 ccacgttgcg ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc gcatccatga
    23341 tggtcagagg aatattggta atcggctgct gacccgattt gctgacttcg ccggtgacgt
    23401 acgctttttg cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg
    23461 aatcgagacg cgctgtaatc tcgttgcgta cctgggtaag tgttttcccg gccactttca
    23521 ggcgaccaat ataggggtag aaaatcgcac catcggcgtt aacccagttg ccggtatcac
    23581 tggcgctacg gtattgccct gcgggggtgg ttagttccgg gtgatcccag accgtaacca
    23641 tcaacacatc accaatcccg atacgatatt catagtttgc gatttgctgc tccagctccg
    23701 ggttagcctg cgacataatc ggtttggcgc gcagctgatc aatcaatcct ggcgtgacgg
    23761 gatagatatt gaccattttg tccaggtcat actgattgtc aggtagttca atgacatcct
    23821 tgttactggt cgataaattc tgaccaggaa caagggtaca tgctgtcaaa gacagaacag
    23881 ccaatgtcag taccgaaaac ttaaaaatgt tctttttcat tggtttatat ttatcagtca
    23941 atgtaatgtt atgaattacg tcaggttatt gtccaaccgg tacttctcgc tctgccgtca
    24001 ttggatcgag ctggtatttg cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc
    24061 acgaaaccac cgcgcggttg cggttaggtc caatggtcat caagtcgaac ggaatcgaga
    24121 tataaaaacc tttactaaag ccgccttcgc cgtagtcatc tttagatacg ttactgattg
    24181 ccgcccatac ccctaccgcc acgccgctgt caaagagttt ggcgacgtcg atcgttgcgc
    24241 ctttatcttt tgccagatat tgcccaacgc taagtttcat cagtacgcca ttgagcgtcg
    24301 gcgggttcca gtaagccgtc acgaaacctg tgggcgtgga ataatcggtg aagcgcatca
    24361 tgttgtccca gtcacgctgc ttcacgtagt taacgtccac acccagcgcc cagctggcat
    24421 ctagcgggcg ataaagaagc tcggaaccga caccggcata catcgtttcc agataaccgc
    24481 catacacctg gccatagaaa ccattgccta agtcggcaaa gtaattcgct tgcaagttgt
    24541 tgagataaac gtcattgcga acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag
    24601 agtccgcggg taacagtgaa gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa
    24661 taccgtcatc aagcagcagg tggtcggtaa accagtagcg ggcgctggac atcagcccca
    24721 gctggaacat atagaaatct tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag
    24781 agtagctaaa gcgatcttca cgaatacggt agcaccgacc aaaggcagaa agatcttctg
    24841 cttcaacacg ttgttgttgc agttgctctg atttaccagg cgctgtgccg gccagctgtt
    24901 tgcgcaggct ggctacgtcg gtttcggtag tcaccatcgc catatgctcg cgcttttgcg
    24961 tcacgctaat cttctcaacg ccttgcggca ggttattcac cagaatccgg ttggcacgat
    25021 cgaccgcttc gcgagagtca cggtatttgt attgctgacc agacatatac agcgtcttat
    25081 cgcgtagctg aatttctggc gcgtcaaagc ccgcgttata cttcagggcg gtaagttggt
    25141 ttgctaccgt ggtgtactgc aaaccttcag actctggcgc aggctgccag gccggttttg
    25201 gcgtatcgcg tagggcaggg cgcagatcgt tgaaattggt ccgtaaagtg aaaccaaaca
    25261 tcaacgtgtt tccgcgttca taactgaggt tgaggtctgc ccagctggca gcgcgataaa
    25321 ctgcaccgac gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca ttctggtaat
    25381 tgttgccgtc gtattcgagt ttcagacgca ggggattcca cggcgtttga tactcaatgc
    25441 cgccaaagat ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt
    25501 gagactctgc gcgatgacaa tatttatcgc tcacccggca aaacgggttg gtaatattgc
    25561 ccgcattacc ggcgtagccc catgccatcc cgagggtgaa atcaaatggc cccgcttgct
    25621 tgctggccac cagatattca ccgtcaaaca aacccgtacc agcaatatca cgtttaccaa
    25681 acgccacttg cggtagccag taaccttctt cccacaggcg aagtttaaaa tcgaatgatt
    25741 tgtctttgta tgactgatcg ccgctgaaat cttcccactg gctatatttg cgtgtgcgca
    25801 catccgtata acgaatggtg ccttccagcc atgggaacag cgccacggag gtggagtaga
    25861 accggtattg atcgttatcc cggtagttga cgctgaattc accttccggt gcgatgcgcg
    25921 catttggcat ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa cccagcggat
    25981 ccgggtaggt taatacttca gcatgacagg cactggagac ggcaatggcc aggcagctta
    26041 aaagataaga attcttcttc atcagtcagg aacacgttgc gttaaaacag acacaatctg
    26101 gtcatttaaa tcagcatatt tttcgggcag tacatgcgca gaaaagccca accaaagctg
    26161 gctgccaggc ggtggttcca catgtctttt attccacagg gcaacagggg cgacgacagt
    26221 ctcaccctca ggcgtaatca ccataacgtt attcttgtct gcgcctgcaa ggcgggggtg
    26281 atcttgcaga taatcggtaa cgctacggcc tgcctgccag ggcaattgcc cagccccaga
    26341 aaccgcaccc agcagagtaa tagtcaccgg acgctgtacg gtatagagcg tatagtcccc
    26401 gaccaacggg ggattgctgt tctcatccac tcgtacaaaa tcagggtcca gtttgaccgg
    26461 taagcgacca gtgatattga gattgagtaa ctgctggcgc acggatttaa tcgttgccgc
    26521 cacatcgtcg tcagcttctg cttcccagga agcaagctgt gccatgacat gttggtagtc
    26581 tttaagcgct ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg
    26641 atcgcgcagt tgcggttgtg tcaccaactg gacaacattt tccacggggc caacggaaag
    26701 tgtctgttgt tcgctaggca gataaatagt caccgttcct tgcgcaaagg catggggtgt
    26761 cattacgtaa agtacgctgg caatgaaata cgactgtaat ttattcatgg tgcgggttta
    26821 agaaacgtca tttctacggg aataacccct gcgcctaaca tttgccgact ttgacgaacc
    26881 tggccagtag ccgaatcgac ccagaatgtg ttttgccact gtttttctgg cagcgtggaa
    26941 gtcacctctt cctgccagac attacacagc acattgtgac ccgcaatatt cagcgtctct
    27001 aatccagcaa aggaaaaagt agaacttagg gtcgcagagc gaaaatggtt gtcttcactc
    27061 cagagaatat cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt acgcagaggg
    27121 gcccgcccgg gatttgtcac ttccagcaga ttattattaa gtttgacggt ctttaaaaga
    27181 cgtccattgt gggtaaccag catggcgtta tcctgggata accatttgct ttgttcttgt
    27241 tctatatatc ccagtaccac aaagattcgt tgcccttcat taaggcgtaa atacatcgtg
    27301 gaataaggca acgcctggat ctgctgagcc gctacagtga tatcctgatt atcgaaaagg
    27361 ctggcgcgaa atgtatcgac catactttgc tggctgtgcg tacatcccgc aagaaatagt
    27421 gcgaaaatcg ataaaataag agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat
    27481 gcaaatctct aaagcccggt acgaatatcg caaccgggta ttatttaact tgaatttcga
    27541 cgcgtttaag cagaattagc gcgtagtgga cgtggtggtt gaggtgttat gagaaccgcc
    27601 atcgccactg tcatttgctg caactactgc caacgtcgcc acaccaacag ccgttgccac
    27661 accgacaact gtcgccgtgc cgacagcgcc agcagccacg ccactagtgg tagccgcagt
    27721 cgtggttgca cctgtagtgg tcgttgtggt gccagtagta cccgtcgttg cctcggtccc
    27781 gtttgctgct tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg ccataaggat
    27841 tgcagaaagc ttgtgtttca taacttttcc tttattcatc gcatggacaa tacgtacgct
    27901 tgagatcagg attgtctact ttgagtataa catccttgtg aaatctccct ttcctcatac
    27961 cgctagcact atatactgcg ggtgggaaag gcgcgcagag ggaaatataa gattgttaac
    28021 gatttccctc tggaaatacg acttgtgagc ataagataaa tcttagaaaa atacgaatta
    28081 aagataagaa aattgttacg aaagctatta attattgttg gtaatatagt ttcaagtggt
    28141 actataactg gctgttaaat ggcatttata actattaggt gcagagatat tcgcttaaat
    28201 ggattagttt cttagtggaa aagtaactga tgttataatg tatggtgttg actattgaac
    28261 tattccaata aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt
    28321 tgccgctgtt ggtcctcgca ggccatttac gcggcaaaat tccacgcgta atcctggtat
    28381 aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg acctggactt ctttacgacc
    28441 gtcggagggg ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt
    28501 cattttacgg gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa
    28561 taaaataaag ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct
    28621 caattatcat gccttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt
    28681 actaaagcag ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac
    28741 taagtggttt ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat
    28801 at
//
LOCUS       DAJTYH010000050        28405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTYH010000050 DAJTYH010000000
VERSION     DAJTYH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO609_002115"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OO609_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6633526.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="OO609_002116"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="OO609_002116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6633527.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="OO609_002117"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="OO609_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6633528.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="OO609_002118"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="OO609_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6633529.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="OO609_002119"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="OO609_002119"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6633530.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6427..7056
                     /locus_tag="OO609_002120"
     CDS             6427..7056
                     /locus_tag="OO609_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6633531.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7195..8616)
                     /gene="melB"
                     /locus_tag="OO609_002121"
     CDS             complement(7195..8616)
                     /gene="melB"
                     /locus_tag="OO609_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6633532.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8719..10074)
                     /gene="melA"
                     /locus_tag="OO609_002122"
     CDS             complement(8719..10074)
                     /gene="melA"
                     /locus_tag="OO609_002122"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6633533.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10357..11265
                     /gene="melR"
                     /locus_tag="OO609_002123"
     CDS             10357..11265
                     /gene="melR"
                     /locus_tag="OO609_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6633534.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11464..13731
                     /gene="adiA"
                     /locus_tag="OO609_002124"
     CDS             11464..13731
                     /gene="adiA"
                     /locus_tag="OO609_002124"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6633535.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14056..14817
                     /gene="adiY"
                     /locus_tag="OO609_002125"
     CDS             14056..14817
                     /gene="adiY"
                     /locus_tag="OO609_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6633536.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14954..16291
                     /gene="adiC"
                     /locus_tag="OO609_002126"
     CDS             14954..16291
                     /gene="adiC"
                     /locus_tag="OO609_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6633537.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16395..18038
                     /gene="eptA"
                     /locus_tag="OO609_002127"
     CDS             16395..18038
                     /gene="eptA"
                     /locus_tag="OO609_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6633538.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18035..18703
                     /gene="basR"
                     /locus_tag="OO609_002128"
     CDS             18035..18703
                     /gene="basR"
                     /locus_tag="OO609_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6633539.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18704..19804
                     /gene="pmrB"
                     /locus_tag="OO609_002129"
     CDS             18704..19804
                     /gene="pmrB"
                     /locus_tag="OO609_002129"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6633540.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="OO609_002130"
     CDS             complement(19780..19869)
                     /gene="pmrR"
                     /locus_tag="OO609_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6633541.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19981..21483)
                     /gene="proP"
                     /locus_tag="OO609_002131"
     CDS             complement(19981..21483)
                     /gene="proP"
                     /locus_tag="OO609_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6633542.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21747..22625)
                     /locus_tag="OO609_002132"
     CDS             complement(21747..22625)
                     /locus_tag="OO609_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6633543.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFDDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="OO609_002133"
     CDS             complement(22622..24850)
                     /gene="crfC"
                     /locus_tag="OO609_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6633544.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25253..25588
                     /locus_tag="OO609_002134"
     CDS             25253..25588
                     /locus_tag="OO609_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6633545.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25746..26189
                     /gene="yjdN"
                     /locus_tag="OO609_002135"
     CDS             25746..26189
                     /gene="yjdN"
                     /locus_tag="OO609_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6633546.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26322..27110
                     /gene="phnC"
                     /locus_tag="OO609_002136"
     CDS             26322..27110
                     /gene="phnC"
                     /locus_tag="OO609_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6633547.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27135..28151
                     /gene="phnD"
                     /locus_tag="OO609_002137"
     CDS             27135..28151
                     /gene="phnD"
                     /locus_tag="OO609_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6633548.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28257..>28405
                     /locus_tag="OO609_002138"
     CDS             28257..>28405
                     /locus_tag="OO609_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR6633549.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7296 a         7418 c         6935 g         6756 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagcc aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtcacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctacgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg atcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaacaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaatcgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagcg cctggcagca tgctgccagg tgatccccct gggccacctc
     6361 ttttgcgatt gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa
     6421 ctcaccatga cccgcacact caagccgtta attcttaaca ctggcgcact ggcgctaacg
     6481 ttaatcctga tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg
     6541 acaccggtga ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg
     6601 ctgctgctct atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac
     6661 acctacgcga aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat
     6721 ccgtatgaca agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa
     6781 attctcgttc gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc
     6841 gtggcgctgg cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg
     6901 ggccagggag cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat
     6961 atgttctgcg cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc
     7021 cgccagctac ggcgctatgg cttgataact ggataacaac gtgcactttg ttgatgccgg
     7081 atgcggcgtg aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag
     7141 acgtggcctg ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc
     7201 tttcacatca ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt
     7261 gcgatattta tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata
     7321 gaaacggaag tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat
     7381 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
     7441 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
     7501 ctgcacggag taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
     7561 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
     7621 aatacccgca atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag
     7681 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
     7741 tgatttaact aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga
     7801 cagataatag gggaacaaat ccgcatcacc gataacatat gagaaataat agatagcaaa
     7861 tccggtaata atgttgctgg ctacattata agcaagagcc atacccaaga ggcatgaaag
     7921 ctgatcgttt ttataaataa gcgcaacgat ggctttaagt gtcagatggc ttccttcagc
     7981 agacgattga ttgtctgacg aaaagacttc atgcacattg cgcagagtga tgatggttga
     8041 aacaataaaa aaggcgatca ataccagagt gaacatctga aagccaaatc cccgatcgcc
     8101 accgccgaca taattaacaa atggtagcgt cacacctgcc gtaacaaagc ctgccagact
     8161 ggcaaaaaaa cgcggataag gaaccagttg ttcgcgttca cgtttatcga gcgtgatggt
     8221 tggaaccagc gaccagaagg gaatatccat aatggtgtaa gtcatgcccc agaggatgta
     8281 ggtcacgcaa acaaagacaa tctgagtagt accttcaaac agatgcgcac taaagaggag
     8341 aaataagatt acagagtttg ccaacgtacc gatcaggatc cagggtttga acttacccca
     8401 tcgcgatcgc gtagcattta caatccatcc cataatcgga tcgttaatag catcccagat
     8461 cctcgccacc agaaacaaag taccgaccaa acccacagac agcccgacga catcggtgta
     8521 gtaatacatg aggtacatat acacaatgcc gatcgcaaaa tccttcccga acgctccaaa
     8581 tccataactg agttttgtag tcattgaaat gctcataggg tatcgggtcg ctgttaccaa
     8641 cgcccgcctt ctgttattgg tatcgtcaga ggtgtgcgcc ggaataaatc ccccggcgca
     8701 gtagcgttta gtcgcgtttt aacggtgcaa ccagcttggc agccagtcgc cgtgggcggc
     8761 aatcaggtcg tcaacaagag catatatttc gtcaatgccc agcacggcgg cagtatgcgg
     8821 gtccatcatc gcggcgtggt aaacacggtc gcgattttcc gtaagaatag cttcggtcag
     8881 cagcgtctgt acgttgatgt tggtttgcat cagggcggcc agatgcgaag gtagcgtacc
     8941 gactttggtc ggctgaatgc cattagcatc aaccagacag gctacttcca cgcaacatcc
     9001 ttgtggcagg ttatcaatca aaccatcgtt acggacgttg ccgtaaatca cactcggctc
     9061 gccagtccag atagcgttca tgattgtgct ggcatattcc cgtgacggtt taatatcaat
     9121 ccgggaggcg tttttatact cctccagctc tttatgccag ttcgccagct gctcgacgca
     9181 gcgtttcggg tactcatcca gcggtacttt ataacgctca atcaaatcct cacgacctag
     9241 cttaataaac cacggcgtat attcggcaaa atgttctgat gattccgtga cgaagtagcc
     9301 cagctttttg aacatttcgt agcgcacaat attctggcag cgagtattac catgaatatt
     9361 tggcttcggt gcctgccctg cttcataagc cgccagcagt tccgggtaga gattcacgta
     9421 actgccgtcg gcggttttgc gctccagctc caggtaaaac gccatatggt tgatacctgc
     9481 gcaacggtaa cgcagcgtag ccgggtcgat attgaggtca cgcgccagct cttccgccgt
     9541 tccctgcacc gaatggcaca gcccgacctg tttgatatac ggatagcggg catacatcgc
     9601 ccaggtattc atcgccattg ggttaacata gttgagcatg gtggcatcgg ggcagacttc
     9661 cgtcatgttc tcgcaaattt gccacagatg cggaatggta cgtagcgcgc gcataatacc
     9721 gcccggcccc aacgtatcgg caatggtttg ttccagacca tgccgcttac agacctcgaa
     9781 atcagtcacc gtgcaaggtt cataaccgcc aatctgaaat gccaccacga caaaatcggc
     9841 atcctctaag gcttctttct gttgggtgtg gcaggtgatt ttgccgctgg cccctgctga
     9901 atccatcagc ttacgcacca caatatgcga ctcttccagg cgggtggggt caatgtccat
     9961 cagggcaata tgcgccgttt tcagcgcctc gcgatggaac acatcaccaa gaatattttt
    10021 aacgaaaatc gtcgaaccag cgccgataaa tgtaattttg ggtgcagaca tcatgcagat
    10081 ctcctggctt gcttgaataa cttcatcatg gcaggcgaat ctgcagaaaa cctccggctt
    10141 ccaggttgag cattcccata aactcagatc ctgcgtgaag cagcagtaaa tctgagttta
    10201 tgggaataaa ttacctgaaa agtagagttt tcggcctcat gatgctcgga cagtcccctc
    10261 gcccctctgg gaagagggtg aaaaccgtgc tcccactcgc agtcatcctc cctcactcct
    10321 gccataattc tgatattcta ggaaagagag ccatccatga atacagatac gtttatgtgc
    10381 agcagcgacg aaaaacagac ccgcagcccg ctgtcgctgt attcggaata ccagcggatg
    10441 gagattgagt ttcgcgcacc gcatatcatg cccaccagcc actggcatgg tcaggtcgaa
    10501 gtgaatgtgc ctttcgatgg cgatgtggaa tacctgatca acaatgaaaa agtgaatatc
    10561 aatcagggtc atatcacgct gttctgggcc tgtacaccgc accaactaac agataccgga
    10621 acctgtcaga gcatggcgat ttttaatctg ccgatgcatc tgtttctctc ctggccgctg
    10681 gataaagacc tgattaacca cgtcactcac ggcatggtga tcaaatcact tgccacacag
    10741 caacttagcc cgtttgaagt acgccgctgg cagcaggaat taaacagtcc gaatgagcaa
    10801 attcgccagc tcgccattga tgaaattggc ctgatgctca agcgatttag cctctctagc
    10861 tgggaaccga ttctggtcaa taaaacctcg cgcacacaca aaaacagcgt ctcgcgccat
    10921 gcgcaatttt atgttagcca gatgctgggc tttattgccg aaaactatga tcaggcgctg
    10981 accatcaacg atgtggctga gcacgtcaaa cttaacgcca actatgcaat ggggatattc
    11041 cagcgggtca tgcaattgac gatgaaacag tacattaccg cgatgcgcat caaccacgtt
    11101 cgcgcgttac tgagcgatac cgataaaagt attctcgata ttgccctgac ggcaggcttt
    11161 cgttcaagta gtcgttttta cagcacgttc ggcaaatatg tcggcatgtc gccgcaacaa
    11221 taccgcaaac ttagccaaca gcgccgccag acgtttcccg gctaaagacg atatcagtat
    11281 cagccaaaaa aatagtttag ccgtcgataa taagcagtga aaataattta aaataatcac
    11341 ataacgtatt gtttataaaa ctaaatattt caaaaatgtt tgtttttcac gcgctttaca
    11401 gcccgaaaag gccggaagat acttgcccgc aacgaagatt ccttcataac cgggtaagca
    11461 atgatgaaag tattaattgt tgaaagcgag tttctccatc aagacacctg ggtcggtaac
    11521 gccgttgagc gtctggcaga tgctttaagc cagcaaaatg ttaccgtgat taaatccacc
    11581 tcctttgatg atggttttgc cattctctct tcaaacgaag ccattgactg cctgatgttc
    11641 agctatcaaa tggagcatcc ggacgaacat caaaacgtca gacaattgat cggtaagctt
    11701 catgagcgcc aacaaaacgt gccggtcttc ctgttgggcg atcgggaaaa agccctcgcc
    11761 gcaatggacc gggatctgct ggagcttgtc gatgaattcg cctggattct ggaagatacc
    11821 gccgacttta tcgccggacg cgccgttgcc gcgatgaccc gctaccgcca gcagctgttg
    11881 ccgccactgt tcagcgcgct gatgaaatat agtgacatcc atgaatattc ctgggcagcg
    11941 ccaggccacc agggcggcgt tggttttacc aaaacacccg ccggacgttt ctaccatgac
    12001 tactatggtg aaaatctgtt ccgtaccgac atgggcatcg aacgaacttc cctcggttct
    12061 ttgcttgacc atactggcgc atttggcgaa agcgaaaaat atgccgcacg cgtatttggt
    12121 gccgatcgct cctggtcggt agtcgtcggt acttccggct ctaaccgcac catcatgcag
    12181 gcttgcatga ccgataacga tgtcgtggtc gttgaccgta actgccataa atccatcgaa
    12241 caaggtttga tgctgacagg cgcgaaaccg gtctatatgg tgccaagccg caaccgctac
    12301 ggcattatcg ggccaatcta tccgcaggaa atgcaacctg aaaccttgca aaagaaaatc
    12361 agtgaaagcc cgctgaccaa agacaaagcc gggcaaaaac cgtcttactg cgtggtaacc
    12421 aactgcacct atgacggcgt gtgttataac gctaaagaag cgcaggatct gctggaaaaa
    12481 acctccgatc gtctgcactt tgacgaagcc tggtacggct atgcacgttt caacccgatc
    12541 tatgccgatc actatgccat gcgcggcgaa cctggcgatc acaacggtcc taccgttttc
    12601 gccacccact ccatccacaa actgctgaat gcgctgtcac aggcttctta tattcatgta
    12661 cgtgaaggtc gtggggcgat taacttctcc cgcttcaacc aggcctacat gatgcatgcc
    12721 accacctccc cgctgtatgc catctgcgca tccaacgacg tggcggtgtc gatgatggac
    12781 ggcaacagcg gcctgtcact gacacaggaa gtgattgacg aagcggttga tttccgtcag
    12841 gcgatggcgc ggctatataa agagttcacc gctgacggta gctggttctt caaaccgtgg
    12901 aacaaagaag tcgtcaccga cccacaaacc ggcaaaacct atgactttgc tgacgcacca
    12961 accaaactgc tgaccaccgt tcaggactgc tgggtaatgc atccgggcga aagctggcac
    13021 ggcttcaaag atattccgga taactggagt atgctcgacc caattaaagt cagcatcctt
    13081 gctccgggaa tgggtgaaga tggtgaactg gaagaaaccg gtgttccggc ggcgctggtc
    13141 actgcctggc ttggtcgcca cggcattgtg cctacccgca ccactgactt ccaaattatg
    13201 ttcctgttct ctatgggcgt aacccgtggg aaatggggga ctctggttaa caccctttgc
    13261 tccttcaaac accactatga cgccaacaca ccgctggcgc aggtgatgcc ggaacttgtt
    13321 gaacaatatc ctgacactta cgcgaacatg gggattcacg atctgggtga caccatgttt
    13381 gcctggctga aagaaaacaa ccctggcgca cggttgaacg aagcctattc cggcctgccg
    13441 gtggcggaaa tcaccccgcg tgaagcgtac aacgcgattg tcgacaacaa tgtcgaactg
    13501 gtatccattg aaaatctgcc aggacgcatc gcggcaaact cagttatccc gtatccgcca
    13561 ggaatcccga tgctgctgtc tggtgaaaac ttcggcgata aaaacagtcc gcaagtaagt
    13621 tatttacgct cgctgcaatc ctgggaccac catttcccta gatttgaaca cgaaactgaa
    13681 gggactgaaa ttattgacgg tatttaccac gttatgtgcg tgaaagcgta accactattc
    13741 cgctgaaggc gtaattgttt aaataacatt acgccgcctg gccttaggcc ttttgagtat
    13801 ggcaacgttt tcataaaaat tgctgcaaac aaaaatgtca tactttttgc gcggccccac
    13861 cccacgcttt tttttgcctg ttatttatcc tgtaaaaaaa tatgtacatg agaaattact
    13921 ataaaaattt gtaatattag taaaactcgt tatttttatg catgtttata ttcatcatgc
    13981 aattatataa ccatttcccg gtatcgcttt gctttagcga gaactggtct tttattcgca
    14041 ctcaggagta catgtatgag gatttgcagc gaccaacctt gtattgtttt attgactgaa
    14101 aaagatgtct ggataagggt gaatgggaaa gaacctatta gccttaaagc taaccatatg
    14161 gcgttattaa attgtgaaaa taatattatc gacgtctcct ctcttaacaa cactttggtt
    14221 gctcatatta gtcacgacat catcaaagat tacctccggt ttctgaataa agatctctcg
    14281 caaataccag tatggcaacg tagcgctacg cccatactca ccctgccatg cctgacgcca
    14341 gacgtctttc gcgttgccgc gcaacacagc atgatgcccg cagaaactga gtcagaaaag
    14401 gaacgaacac gcgcattatt attcacggtg ctatcccgtt ttctcgacag taaaaaattc
    14461 ctttcattaa tgatgtatat gttacgtaat tgtgtaagtg acagcgttta tcaaattatt
    14521 gaaagcgata ttcacaaaga ctggaatctt agtatggtag ccagttgttt atgtcttagc
    14581 ccaagtctgt taaagaaaaa gttgaaaagc gaaaacacca gttatagcca aataatcacc
    14641 acctgccgca tgcgttatgc cgtaaatgaa ttaatgatgg acggtaaaaa tatctctcag
    14701 gtatcacagt cctgcggcta caacagtacg tcgtacttta tttctgtctt taaagacttc
    14761 tacggtatga cgccgctgca ttatgttagt cagcacagag aacgcactgt cgcctgattt
    14821 ttaaccttaa cgaagagcta tattaataac ggcatcagcg ataacccggt cgataataat
    14881 tcaactatcg aatgcaggcg tatgatatga cgtaatttat tgtcacgaag ctcgccttcg
    14941 caggagttta attatgtctt cggatgctga tgctcacaaa gtgggcttaa tccccgtcac
    15001 cctgatggtg tcggggaata ttatggggtc aggtgttttt ctgttacctg caaacctggc
    15061 ctccactggc gggattgcca tttatggatg gttggtgacg attatcggtg cgctggggct
    15121 ctcgatggta tacgccaaaa tgtcgttcct cgacccaagt cctggtggtt cttacgctta
    15181 cgcccgccgc tgctttggcc cgtttctcgg ttatcaaacc aacgtcctct actggctggc
    15241 ctgctggatc ggcaatatcg ccatggtggt cattggcgta ggatatttaa gttacttctt
    15301 cccgattctg aaagatccat tggtattaac catcacctgc gtcgtggtgc tgtggatctt
    15361 cgtcctgctg aacattgtcg gtccgaaaat gatcacccgc gtgcaggccg ttgccactgt
    15421 gctggcgctg atccccatcg tcgggattgc cgtatttggc tggttctggt tccgtggtga
    15481 aacctatatg gcggcatgga acgtcagcgg cctgggcacc ttcggtgcga ttcaaagtac
    15541 ccttaacgtt acgctgtggt cgttcatcgg tgtggaaagt gcctccgttg ccgcaggtgt
    15601 ggtgaaaaac ccgaaacgca atgtccctat cgccaccatt ggtggggtat tgattgccgc
    15661 cgtttgctat gtgctctcca ccaccgcgat tatggggatg atccctaatg ccgcactgcg
    15721 cgtttctgcc tcgccattcg gtgatgccgc acggatggcg ttgggtgaca ccgctggggc
    15781 cattgtttcc ttctgcgcag ctgcgggttg cttaggttca ctgggcggct ggacgttgct
    15841 ggcgggtcaa acggcgaaag ccgctgccga tgacggactg ttcccaccga tttttgcccg
    15901 tgtaaataaa gcgggtacgc cagtggcggg gctgattatc gtcggtattt tgatgaccat
    15961 cttccagctc agcagcattt caccaaacgc gaccaaagag ttcggtctgg tttcttccgt
    16021 ctcggtcatc tttacactgg tgccatatct ttacacctgt gcggcgttac tgctgctcgg
    16081 ccacggtcac tttggtaaag cacgcccggc atatctggct gttactacca ttgccttcct
    16141 ctactgcatt tgggccgtgg tggggtccgg ggcgaaagag gttatgtggt catttgtcac
    16201 cctgatggtc atcaccgcca tgtatgcgct gaattacaac cggctacata aaaacccgta
    16261 tcccttagat gcaccaataa gcaaagatta attcccctta atccagcaaa cataaaagcc
    16321 aaccttaaga acttaaggtt ggcttaattt tgctttgcga gcatatgcgc actttgttcg
    16381 atggaaacac cgtgatgttg aagcgcctac taaaaagacc ctctttgaat ttactcgcct
    16441 ggctattgtt ggccgctttt tatatctcta tctgcctgaa tattgccttt tttaaacagg
    16501 tgttgcaggc gctgccgctg gattcgctgc ataacgtact ggttttcttg tcgatgccgg
    16561 ttgtcgcttt cagcgtgatt aatattgtcc tgacactaag ctctttctta tggcttaatc
    16621 gaccactggc ctgcctgttt attctgattg gcgcggctgc acaatatttc ataatgactt
    16681 acggcatcgt catcgaccgc tcgatgattg ccaatattat tgataccact ccagcagaaa
    16741 gttatgcgct gatgacaccg caaatgttat taacgctggg attcagcggc gtgcttgctg
    16801 agctgattgc ctgctggcta aaaatcaaac ctgccacctc gcgtctgcgc agtgttcttt
    16861 tccgtggagc caatattctg gtttctgtac tgctgatttt gctggtcgcc gcactgtttt
    16921 ataaagacta cgcctcgttg ttccgcaata acaaagagct ggtgaaatcc ttaagcccct
    16981 ctaacagcat tgttgccagc tggtcatggt actcccatca gcgactggca aatctgccgc
    17041 tggtgcgaat tggtgaagac gcgcaccgca acccgttaat gcagaacgaa aaacgtaaaa
    17101 atttgaccat cctgattgtc ggcgaaacct cgcgggcgga gaacttctcc ctcaacggct
    17161 acccgcgtga aactaacccg cggctggcga aagataacgt ggtctatttc cctaataccg
    17221 catcttgcgg cacggcaacg gcagtttcag taccgtgcat gttctcggat atgccgcgtg
    17281 agcactacaa agaagagctg gcacagcacc aggaaggcgt gctggatatc attcagcgag
    17341 cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg taaaggtgcc tgcgaccgcg
    17401 tgcctcacca gaacgtcacc gcgctgaatc tgcctgatca gtgcatcaac ggcgaatgct
    17461 atgacgaagt gctgttccac gggctggaag agtacatcaa taacctgcag ggtgatggcg
    17521 tgattgtctt acacaccatc ggcagccacg gtccgaccta ttacaaccgc tatccgccgc
    17581 agttcaggaa atttacccca acctgcgaca ccaatgagat ccagacctgt accaaagagc
    17641 aactggtgaa cacttacgac aacacgctgg tttacgtcga ctatattgtt gataaagcga
    17701 ttaatctgct taaagaacat caggataaat ttaccaccag cctggtttat ctttctgacc
    17761 acggtgaatc gttaggtgaa aatggcatct atctgcacgg tctgccttat gccatcgccc
    17821 cggatagcca aaaacaggtg ccaatgctgc tgtggctgtc ggaggattat caaaagcggt
    17881 atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac gcaacactat tcacaagaca
    17941 atttattctc cacgctattg ggattaactg gcgttgagac gaagtattac caggccgcgg
    18001 atgatattct gcaaacttgc aggagagtga gtaaatgaaa attctgattg ttgaagacga
    18061 tacgctgtta ttgcagggac tgattctggc ggcgcaaacc gaaggctacg cgtgcgatgg
    18121 cgtgacaacc gcgcggatgg cggaacaaag ccttgaggac ggtcattaca gcctggtggt
    18181 actggattta gggttacccg acgaagatgg actgcatttt ctcgcccgta tccggcagaa
    18241 aaaatacacc ctgccggtac tgatcctcac cgctcgcgat acgctgaccg acaaaatcgc
    18301 cgggctggat gtcggtgccg atgactatct ggtgaagcct tttgcgctgg aagagttaca
    18361 cgcccgtatc cgcgccctgc tacgacgcca taataatcag ggcgaaagtg agctgattgt
    18421 tggcaatctg acgctgaata tgggtcgccg tcaggtatgg atgggcggtg aagagttgat
    18481 tctgacgccc aaagaatatg ctctgctgtc acggttaatg ctcaaagcag gcagtccggt
    18541 acatcgggaa attctctaca acgacatcta taactgggac aatgaaccct cgaccaacac
    18601 cctggaagtg catatccaca atctgcgcga caaagtgggc aaagcccgta tccgcaccgt
    18661 gcgcggcttt ggctatatgc tggtcgcgaa tgaggaaaac taattgaatc tgatgcgttt
    18721 tctgcgccga cctatatcgc tgcgccaacg gctgatattg accatcgggg ccattttgtt
    18781 ggtgtttgag ctgatcagcg tcttctggct atggcatgaa agtaccgagc agattcagct
    18841 gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc cacatcatgc gtgagatccg
    18901 cgaggcggtc gccagcctga ttgtccccgg cgtctttatg gtcagcctga cgctatttat
    18961 ctgctatcag gcggtacgcc gcatcacccg cccgctggcg gagctgcaaa aagagctgga
    19021 agcgcgcacc gccgacaact taacgcccat tgccattcac agcgccaccc tcgaaatcga
    19081 agcggtggtt tcggcgttga acgatctggt cagtcgcctg accagcacgc tggataacga
    19141 aaggttgttt accgctgacg tcgcgcacga actgcgaacg ccactggcgg gggtgcgttt
    19201 gcatctggaa ctgctggcga aaacgcatca cattgatgta gcaccgttag tggcacggct
    19261 tgatcagatg atggagagcg tctcccagct gctgcaactg gcgcgtgccg gacagtcatt
    19321 ttcttccggt aattatcaac atgtaaaact gctggaagat gtcattctcc cctcgtatga
    19381 cgaactcagt accatgctcg accagcgaca gcaaaccctg ctattgccag agagcgccgc
    19441 cgacatcacc gttcagggtg atgcaaccct gctgcggatg ttattgcgaa acctggtaga
    19501 aaacgcccat cgttacagcc cgcaaggcag caacattatg attaagctgc aagaagatgg
    19561 cggagcggtc atggcagttg aagatgaagg accaggtatt gatgagagta aatgcgggga
    19621 gttgagtaaa gcgtttgtac gtatggacag ccgttatggc gggattggtc tggggttaag
    19681 tattgtcagc cgcattacac agttgcatca cgggcagttt ttcctgcaaa accggcaaga
    19741 gacttccggc acgcgggcct gggtacggct gaagaaagat cagaacgtgg caaaccagat
    19801 ataaagaaag ctgctgacca ccagcacgct gaacacggta gttaaacttt cataaacacg
    19861 gtttttcatt gcactctcct ccgttaacct ggaggagagt atgcgcgtca gagattaagt
    19921 gaaccttaag agttcaattg gcctgataag acagcgtcac atcaggccat ccgtttcagc
    19981 ttattcatca attcgcggat gttgctgcac caggcgggta cgtttcgcct gcaaatcggc
    20041 aatctcgtgg tcaatattat cgattttctg ctcgatatta tcgtaatgct cgacgagaat
    20101 ttccttcgct tcctgtatat ctgacgccgc cggtgtcgca cctttcaacg gacgatttgc
    20161 cgtctctttc atggttacgc cggtgattaa accaatcacc gccactacca tcaggtaata
    20221 ggcaggcatc atcagattct gcgagctttc gaccagccag gccgccagtg ttggcgtcag
    20281 accggcaacc agcaccgaaa tattaaatgc cgccgccagc gcgctgtaac ggatatgcgt
    20341 cgggaacatc gctggcaagg tagaagccat aacgcccata aagcagttaa ggatcaccgc
    20401 cagcatcagt aacccggcaa aaatcaggcc gatgacgtta ctgttaatca gaataaacgc
    20461 cgggatcgcc aacacaaaca gtgcaacact accaagtagc acaaacggac gacggccaaa
    20521 acggtcactc agcaagccca tcaccggctg gacaaacagc ataccgatca taatggcgat
    20581 aataatcagc accccgtggt cttcggagta atgcaggtta tgcgacaaat aactcggcat
    20641 ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt accagaccaa tacatgtcaa
    20701 caggctgcgc cagtatttag tggcaatctc tttaaacgag actttcgggc catcctgcaa
    20761 accttcgcgg tcgccctgtt ccagtttatc aacatgctgc tggaacgccg gagtttcttc
    20821 cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc agagcaataa agaacggaat
    20881 acgccagccc cagtcgagga agttcgcttc gccgacaatg gtcgaaatta acaccaccac
    20941 gcccgcaccc agcacaaacc cggcaataga accgaagtcc agccagctgc ccataaagcc
    21001 acgtttacgg tcaggggagt attccgcaac aaatatcgac gccccggtat attcaccacc
    21061 gaccgagaaa ccttgtgcca tcttacagat caacagcaga atcggtgccc aaataccaat
    21121 cgtgtcgtag gacggtatta agccaataca gaacgtactg atcgacataa tcacaatagt
    21181 gatagcgagg atcttctggc gaccatattt atcgcccaac ataccaaaga agagtccgcc
    21241 aagcggtcga atcagaaagg gaacggagaa agtggcaagt gcagcaacca tctgcacgct
    21301 ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa gcaacaaaac cataaacacc
    21361 aaaatcaaac cattccattg cattacccag tgatgctgcg gtaatggctt tacgcagttt
    21421 accgtcatca ataatggtga catcacgaag ggtaatcggt tttacttttt tccttttcag
    21481 catagctttc ctcgcagagt tggcccattt ccgcaaacgc cagaaaaatc agcgcgacgg
    21541 ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt aacaagcgta gcgggtttgc
    21601 ttacaccctc cggttaagga atgtacaatc aaaccagttg acgcctgaat tgggcagtta
    21661 atgacctttt taccctacca gtgtttaaat ttgtgatcaa cttcacacat ggatttcgtt
    21721 taggactcat tgatgtaact gattatttac gccaaattta cagagaagat ttcctcgacc
    21781 attgccccca ccaaacgctg cgctgaacag agtcgtggca tccccagcgc gacagtttgc
    21841 cagcgttggg tcactgacca gcaaacgggt tgtttatctg ccgtggtcca ctttcccagc
    21901 caacttaaaa aatcagcatg atccccgccc gtcggaacgg tcgaagaggc aaaccactga
    21961 tggtaatagt gacgcgtagt cggtgccgca ttgatactct ctgccagatg ttgtgtcgct
    22021 ttctcgcgca aatcacccag caacaacacc accgcttgcg ggcttcccag acggcaggca
    22081 tcgtcaaatg caccccagcg caactcctcc agaacgataa aacagcgccc aggcaacgac
    22141 caggcgttat acgccccagc acgccagcgg gtaaaaattt gctcgctctg atcacctgcc
    22201 tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc agcgacaacg ggctgaaaac
    22261 cactcataca aggcggcaat ttgctggggg agctccggga aagcagagtg ctggcggtga
    22321 tccatttttt gcagcagcgc cagatgctgc gcgatctgcg tcagcgccag atgaccggga
    22381 tccagcatcg acgccagttt ttccaccatt gtcagtcgca ggctaagcgg cgcatttaac
    22441 atgccgccgt tcgtccatgt ctgaagctgc acacgcgaaa gaagttcctg catcagtcgt
    22501 tcacgaaact gctgctgatg ggcttgagga tggcgggcat catcgccctg cgccagatcc
    22561 actaaaaagc gcggatgaac cgactccagc acgcgaccgg ggccgtccag taacgtcttg
    22621 gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc gcgtaacagc tgagcgtcgg
    22681 tgtaaatcca gctgtgagtc atcacagtct gctgcaattg ctgctgcatc agcctgacca
    22741 ccgattcatt ttgttgacgc agagccagac tttcgcgtaa acgcgtctgt aattctgtca
    22801 aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc cgtaacagat tcatcgacct
    22861 gctctgccag aactttagcc atttgttggc aatagagatc gacttctgcg cttaatgctc
    22921 gttgcaacac actgtaatca accgtttctg tcggggattt ctcatttccc cgtccccagt
    22981 cgggctgatt caaccagcgc gaaaaagtct cacgcacaac gcctaaacgc gtgctctgct
    23041 cgtccgttgc ttcctggcgc gaaatgactt cactgaacag ctggcgagtg ttgaagtggg
    23101 gaactacgcc gtgaaaaaca ggaaaatgaa acccaggacg aaaccctgac tcgctcaatt
    23161 ccattttgac ttgttgctca atggggcgaa taacatcggt taacacccgg caaagggtgg
    23221 attccatctc ggcaaaacgc agcgtaaagt cgcgactgat ggtgttctgc gccgtctgta
    23281 acagtgtctc acagcgggta cgaatctcgc ttaacggctc cgaatcatcc tgaaacaagg
    23341 cggctaactg cgcattcacc gcatcttgct gttgacgcag aaagtggttg gcggaggtca
    23401 gggccagctc gatttcatgt ttaatctcgc cgctcacctg cgcctgattg agttgcaata
    23461 gctgcaaact ttcttcgacc tgatggatat tttgccgcaa ttgttcacaa gcgacgttta
    23521 acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg cgcgtaattc aacagtttat
    23581 gcgcagcaga tcgcagagca tacaatgagg cgttagcgta agcggcatga agcaacgcct
    23641 gaattggctg ggcgaacagc gaatcttccc acaactgatc ggcagcatga cgaatatgtt
    23701 cgaggtccgc cagatcggca tgacgccagc gcctgccgag cgcggcatgg gcaaaatctt
    23761 ccacccagcg ttgttgctct ggcgctggta acttaccgtt gttggctaac tcatagcgcg
    23821 cccgattcgc caggtagccc cacatcgacg acaccgggaa tatctgctgt ggcgtaatac
    23881 agcctttcat cagcgtcccg gaaatcagtg cccgcacctg gtcggcgtcg tcactgttac
    23941 gatcctgttg atcgaactta ttgaccagca catacagcgg caccgattgc cccaccgcca
    24001 aaatcgcctc acggacctct tcatcggaga tcgatttcag ttgcgtataa tccagcaccg
    24061 ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat tttttgcaga tgcggttgcc
    24121 cggcttcatt tggcccgggg gtatccagta acgtcaactg accgggataa ctttccagcc
    24181 ccgccagatg gacaaactcc acttcaatca cgggaatatg ctcaatggcg gcgtaagcag
    24241 aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac taaatcattc aaacttttca
    24301 gacaatgaaa aataggctgg gcacccagat aatatttttc gaaagcgacg ccattttcga
    24361 tccgctgcat aagcgcacgc atatctttat ctatttccag cacatcggtc agatgcttaa
    24421 tatcgcaatc acgcaggcgc tgttgtaatt gttgaattaa acaatcgatt ggcgcgacat
    24481 gtgaaaaatg cagtaccggt tccttttgcc cgggcgtatg gcgaataagc gtcggcagcg
    24541 cagtcattgg gcgattacga ttaggcagaa cctccgtacc aacaatggca ttaatggtgg
    24601 ttgatttccc tgctttcatg gtaccgacaa ttgccagcac catttccagt cgggaaattt
    24661 tacacaactc attattcagc atcgcgtgac gttcggcgat attaggctga ctccagggta
    24721 aagccagttg tggcgcgtcg tctccgggta cagagagagg cattttttcc agtaactgca
    24781 actgttggcg agaaagctgt aacaggcgtt cagcctcctg acttaactca tacagggtct
    24841 gtgtgtacat agaaaattct tccttaaagc aaattttgtt acttttattt agccagattg
    24901 tttttgagtt ctgttttcgg cttttataat tactgcaaga aataatttta tatttagtgt
    24961 gttgtttttt tatcagaata aataacgtct tctgatgcgt ttaaaacgtc agaaagataa
    25021 aaatatcatg tgaataaaaa aaagaacaag tagagcatta acattatctt aaataataaa
    25081 tagaggcaaa aagattattt tctttttgcg tttcctttca aatgaaaacg atcgtcgtct
    25141 aaaatcagca gtacccccga caaactcagg gattttgtgt ataattgcgg cctttttcgg
    25201 caatctgccg ttttttggcg cttttgccct gctgactttt gaggaaatcc acatgtcatt
    25261 accacactgc ccaaaatgca actccgaata cacttacgaa gataacggca tgtacatctg
    25321 cccggaatgt gcctacgaat ggaacgacgc agaacctgca caggaaagcg acgagctgat
    25381 cgttaaagat gctaacggca atctgctggc tgacggcgac agcgttacca tcattaaaga
    25441 tctgaaggtg aaaggtagct cttcgatgct gaaaattggc accaaagtga aaaacatccg
    25501 cctggttgaa ggcgaccata acatcgattg caaaatcgac ggttttggtc cgatgaaact
    25561 gaaatctgag tttgtgaaaa agaactgatt gtattgtgat cggtaagccg gataaggcgc
    25621 tcgcgccgca tccggcaacg atgccgactg cctgatgcga cgcttgcgcg tcttatcagg
    25681 cagcctctca tctgtataaa tttcgaacta cacttaactg gcttctctta actgaggtca
    25741 ccatcatgcc gttaagtccc tacctctctt ttgccggtaa ctgttccgac gcgattgcct
    25801 attatcaacg tacgttgggc gcggaactgc tctataaaat cagcttcggc gaaatgccga
    25861 aatcagcgca ggacagcgcc gagaactgcc cttccggaat gcaatttccc gataccgcca
    25921 tcgctcatgc caacgtgcgc attgccggaa gcgacatcat gatgagcgat gctataccgt
    25981 caggaaaagc cagctactcc ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag
    26041 gaaaacgctg gtttgataac cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa
    26101 ctttctgggc gcatggcttt ggcaaagtca ccgataaatt tggcgtaccg tggatgatta
    26161 atgtcgtcaa acaacaacca acgcaataac ccgccgggag gcccgccctc ccgcactgtc
    26221 atcgaattcc cgttaactct tcatctgtta gtcactttta attaaccaaa tcgtcacaat
    26281 aatccgccac gatggagcca cttttttagg gaggctgcat catgcaaacg attatccgtg
    26341 tcgagaagct cgccaaaacc ttcaatcagc atcaagcgct gcatgcggtt gatctgaaca
    26401 ttcatcacag tgaaatggtg gctctgcttg ggccgtcggg ttccggcaaa tccacccttt
    26461 tacgtcattt aagcggtttg attaccggcg ataaatccgc cggtagccat atcgagctac
    26521 tgggccgcac tgtccagcgc gaaggccgcc tggcccgcga tatccgcaaa agccgcgcca
    26581 acaccggcta catctttcaa caattcaacc tggtgaaccg cctgagcgta ctggagaacg
    26641 tgctgattgg cgcgctcggc agcacgccgt tctggcgcac ctgttttagc tggttcaccg
    26701 gcgagcagaa acagcgcgcg ttacaggcgc tgacccgcgt tggcatggtg cattttgccc
    26761 atcagcgcgt ttccaccctc tccggcggcc agcagcaacg tgtggcgatt gcccgtgcgc
    26821 tgatgcagca ggcgaaagtg attctggccg atgaacccat cgcctcgctg gacccggaat
    26881 cagcgcgcat cgtgatggac accctgcgcg acatcaacca gaacgacggc atcaccgtgg
    26941 tcgtcacgct gcatcaggtg gattacgccc tgcgctactg cgaacgcatc gtcgccctgc
    27001 gtcaggggca cgtcttctac gacggcagca gccaactgtt tgataacgaa cgttttgacc
    27061 atctctaccg cagcattaac cgcatcgaag agaacgcgaa agctgcctga tatccccatc
    27121 attgaggaaa acgaatgaac gctaagataa ttgcctcgct ggccttcacc agcatgttca
    27181 gcctcagcac cctgttaacc ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg
    27241 gcattatttc aacggaatca cagcaaaacc tgaaaccgca atggacgccg tttttacagg
    27301 atatggagaa gaaactgggc gtgaaggtca acgccttctt tgccccggac tacgcgggca
    27361 ttatccaggg gatgcgcttc aataaagtgg atattgcctg gtacggcaac ctgtcggcga
    27421 tggaagcggt ggatcgcgcc aatggccagg tcttcgccca gacggtcgcg gcggatggat
    27481 cgccgggtta ctggagcgtg ttgatcgtca acaaagacag cccgatcaac aacctgaacg
    27541 atctgctggc gaagcggaaa gatctcacct tcggcaatgg cgatcctaac tccacctctg
    27601 gcttcctcgt ccccggttac tacgtcttcg ctaaaaacaa tatctccgcc agcgacttca
    27661 agcgcaccgt caacgccggg catgaaacca acgcgctggc cgtcgccaac aagcaagtgg
    27721 atgttgccac caacaacacc gaaaacctcg acaagctgaa aacctccgcg ccggagaagc
    27781 ttaaagagct gaaggtgatc tggaaatcgc cgctgatcct aggcgatccg atcgtctggc
    27841 gcaagaatct gtccgaaact accaaagaca agatctacga cttctttatg aactacggaa
    27901 aaacgccgga agagaaagcg gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca
    27961 gcgacctgca actggtgccg attcgccagc tcgcactgtt taaagagata caaagcgtga
    28021 aaggcaataa agggctgaat gagcaggaca agctggcgaa aaccaccgag attcaggcgc
    28081 agctggatga cctggaccgc ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag
    28141 cggtgcagta aatcgtaggt cggataagac gccccggcgt cgcatccgac aatgtgtagg
    28201 cgttgatgcc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
    28261 aaaccatcac catcgcccca cccaagcgta gctggttctc gcttctgagc tgggccgttg
    28321 ttctcgacgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
    28381 aagacggcgg caacatggca acctt
//
LOCUS       DAJTYH010000051        28120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTYH010000051 DAJTYH010000000
VERSION     DAJTYH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(67..1359)
                     /gene="spaL"
                     /locus_tag="OO609_002139"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             complement(67..1359)
                     /gene="spaL"
                     /locus_tag="OO609_002139"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6633550.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            complement(1363..1764)
                     /gene="spa15"
                     /locus_tag="OO609_002140"
                     /gene_synonym="spaK"
     CDS             complement(1363..1764)
                     /gene="spa15"
                     /locus_tag="OO609_002140"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6633551.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            complement(1777..3837)
                     /gene="mxiA"
                     /locus_tag="OO609_002141"
                     /gene_synonym="virH"
     CDS             complement(1777..3837)
                     /gene="mxiA"
                     /locus_tag="OO609_002141"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6633552.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            complement(3850..4917)
                     /gene="mxiC"
                     /locus_tag="OO609_002142"
     CDS             complement(3850..4917)
                     /gene="mxiC"
                     /locus_tag="OO609_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6633553.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            complement(4936..6636)
                     /gene="mxiD"
                     /locus_tag="OO609_002143"
     CDS             complement(4936..6636)
                     /gene="mxiD"
                     /locus_tag="OO609_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6633554.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            complement(6623..7255)
                     /gene="mxiE"
                     /locus_tag="OO609_002144"
     CDS             complement(6623..7255)
                     /gene="mxiE"
                     /locus_tag="OO609_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6633555.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(7385..7813)
                     /gene="mxiM"
                     /locus_tag="OO609_002145"
     CDS             complement(7385..7813)
                     /gene="mxiM"
                     /locus_tag="OO609_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6633556.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(7797..8204)
                     /gene="mxiM"
                     /locus_tag="OO609_002146"
     CDS             complement(7797..8204)
                     /gene="mxiM"
                     /locus_tag="OO609_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6633557.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(8197..8892)
                     /gene="mxiN"
                     /locus_tag="OO609_002147"
     CDS             complement(8197..8892)
                     /gene="mxiN"
                     /locus_tag="OO609_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6633558.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(8834..9361)
                     /locus_tag="OO609_002148"
     CDS             complement(8834..9361)
                     /locus_tag="OO609_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6633559.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(9358..10083)
                     /gene="mxiJ"
                     /locus_tag="OO609_002149"
     CDS             complement(9358..10083)
                     /gene="mxiJ"
                     /locus_tag="OO609_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6633560.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(10089..10382)
                     /gene="mxiI"
                     /locus_tag="OO609_002150"
     CDS             complement(10089..10382)
                     /gene="mxiI"
                     /locus_tag="OO609_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6633561.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(10395..10646)
                     /gene="mxiH"
                     /locus_tag="OO609_002151"
     CDS             complement(10395..10646)
                     /gene="mxiH"
                     /locus_tag="OO609_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6633562.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(10654..11769)
                     /gene="mxiG"
                     /locus_tag="OO609_002152"
     CDS             complement(10654..11769)
                     /gene="mxiG"
                     /locus_tag="OO609_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6633563.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(11778..12236)
                     /gene="ipgF"
                     /locus_tag="OO609_002153"
     CDS             complement(11778..12236)
                     /gene="ipgF"
                     /locus_tag="OO609_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6633564.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(12236..12598)
                     /gene="ipgE"
                     /locus_tag="OO609_002154"
     CDS             complement(12236..12598)
                     /gene="ipgE"
                     /locus_tag="OO609_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6633565.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(12608..14224)
                     /locus_tag="OO609_002155"
     CDS             complement(12608..14224)
                     /locus_tag="OO609_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6633566.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            14538..16022
                     /gene="icsB"
                     /locus_tag="OO609_002156"
     CDS             14538..16022
                     /gene="icsB"
                     /locus_tag="OO609_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6633567.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            16035..16424
                     /gene="ipgA"
                     /locus_tag="OO609_002157"
     CDS             16035..16424
                     /gene="ipgA"
                     /locus_tag="OO609_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6633568.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            16440..17066
                     /gene="ipgB1"
                     /locus_tag="OO609_002158"
     CDS             16440..17066
                     /gene="ipgB1"
                     /locus_tag="OO609_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6633569.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            17123..17590
                     /gene="ipgC"
                     /locus_tag="OO609_002159"
     CDS             17123..17590
                     /gene="ipgC"
                     /locus_tag="OO609_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6633570.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            17596..19338
                     /gene="ipaB"
                     /locus_tag="OO609_002160"
     CDS             17596..19338
                     /gene="ipaB"
                     /locus_tag="OO609_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6633571.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            19358..20449
                     /gene="ipaC"
                     /locus_tag="OO609_002161"
     CDS             19358..20449
                     /gene="ipaC"
                     /locus_tag="OO609_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6633572.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            20500..21498
                     /gene="ipaD"
                     /locus_tag="OO609_002162"
     CDS             20500..21498
                     /gene="ipaD"
                     /locus_tag="OO609_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6633573.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            21507..23408
                     /gene="ipaA"
                     /locus_tag="OO609_002163"
     CDS             21507..23408
                     /gene="ipaA"
                     /locus_tag="OO609_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6633574.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            23412..23648
                     /locus_tag="OO609_002164"
     CDS             23412..23648
                     /locus_tag="OO609_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6633575.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            23865..24794
                     /gene="virB"
                     /locus_tag="OO609_002165"
     CDS             23865..24794
                     /gene="virB"
                     /locus_tag="OO609_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6633576.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(25741..26520)
                     /gene="ipaJ"
                     /locus_tag="OO609_002166"
     CDS             complement(25741..26520)
                     /gene="ipaJ"
                     /locus_tag="OO609_002166"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6633577.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(27120..27257)
                     /locus_tag="OO609_002167"
     CDS             complement(27120..27257)
                     /locus_tag="OO609_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633578.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            27873..>28120
                     /locus_tag="OO609_002168"
     CDS             27873..>28120
                     /locus_tag="OO609_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6633579.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT         9543 a         5744 c         4123 g         8710 t
ORIGIN      
        1 agtgttaata tgtgtgcttc aattaattta acagaccgtt gatatttatc tttgattttt
       61 aaaaccttat ctaattgttt caccaataag ctccattgtc tgctcataag taaaaccgag
      121 tcgataatct tgcttcagaa aactctcaac aacagatatt ttgttgtaga ttttatcctg
      181 agaggcattc tcccccggtt tgtattcacc aaagtcaata attgtcctta gctcttctat
      241 ttcagaaagt agctcccgaa atgcagcggc cataatacga tgtttttcat caacaacctg
      301 tgtaaatacc ctgcttatac tttttaagga atcaattgca ggaaattgtc ctttttgggc
      361 tagatttctg ctcaaatata tatgtccatc taaaatggat cttacctctt cagctaatgg
      421 atcagcaaaa tcatcatcct ccaaaagaac agtgtaaaat gcagtaatag agccacctgc
      481 ctttaacttt cctggccttt caagaagtct gggtaagcta tcaaaaaccg aaaccggata
      541 gcctcttctg gcaggtgatt ctccagcggc taaggccaca tcacgtaatg ctctggcata
      601 ccttgttaat gaatcaataa aaagcgctac tttatgtcct tcagtcctaa aaaattcggc
      661 tatagcagtg gctatatatg cagcattaca acgatcaacc gaagagtaat ccgaagttgc
      721 atatactaaa acacacctgc tttttttctc agagtttttc aaataatcaa ccgtttcagt
      781 aacctctcga cctcgctcac caattaaccc aataacatat atatcagcac cactatgttc
      841 aatgagcata ttcatgagaa aagttttgcc acaaccagct gacgcaaaaa tccccattcg
      901 ctgtccttca ccacacgtga gtaaagaatc aataacctta atacctgtta aaaaaggctt
      961 ctcaattgca gccctttcac tatatagcgg aggagcatta tctacaggtc gataaagaat
     1021 ttcactgtta tctgtaacag caaacttatc agtaacctcc cctaaaggat tgactactgc
     1081 tcccaataat ccacggccga cttgcgtatg aagaaactgg gcagtgggct taatcaacgt
     1141 ttgccgtgaa agtccacgag aatttccaat caagcttaat attgtttttt catcatgaaa
     1201 tcctacaacc tgagctcttg caacaatttc attactttca attccagcct gaatgttaca
     1261 aatctcacca atcgaaacat cgctaagact tgtttccaag attggccccg agattctatt
     1321 aggaaaagat aattgagtga gcaattttgt atagctcatt gattataaga ccccatttaa
     1381 gatttccatc ctctgataaa actcatgcag aatctctgca aaaacaatcc catcgtgaac
     1441 gtagtcatct ttaataacaa ctcttaactg taagtactca tcagaacgat gaagctctac
     1501 cagctcatta atgctataac taaagttcat taacattaaa tttaatatgt tataagcaga
     1561 tgactgaagt tttacatcac ttggtgcatc aaaattagcc cataacataa cctgctcatt
     1621 gacaagagct atattaatag caggcatact atccaaagaa atggtaatcg ctgaatggct
     1681 gtctaaatct gtaataattg atggaggaca accaatcgtg aaaagactat ctctaactaa
     1741 ttgaactaaa ttaatgttac tcatatttaa acctcactaa atagtcttta atacattaat
     1801 ggtatatgct tcatcaatct cagcatacga tataacgagt atactcttaa atctgttatc
     1861 tataagtctt ttaacaaacc tacgtatatc tactgatacc aaaagaacga aatttttttt
     1921 tgcatttctc aattctctca aagcatgtgc taaagtttcc attacctcat ccgaaacctc
     1981 tatatccata ttcaagaaag agccaccaga ggtttgcctt atcccttttc ttattgcatc
     2041 ctcaatatat ccggaaagca tcacaacttt aatctcacca gaaacagcta ttttactaca
     2101 aatataccta gaaagtgatg cacggacatg ttcaactaat gttatgacat ctttttctct
     2161 gggagcccaa agcgccaaag actccataat aagttttaaa ttgcgaacag aaatattttc
     2221 tccaagcaat ctttgtaata cctcagaaat tctctgtata gtcacatgtc ggaagacttc
     2281 ctttaataga tcaggatacc gattttcaaa ctgatctaac atattttttg tttcttgtat
     2341 accaaaaatc tcatttatgt tgtttaataa agcttgtgac aactggtgat aaaattcatc
     2401 ttgagcactt ttaattttag catcaatatt agttagattt tctgtatatg aaacgtctac
     2461 ccaagaaata acacgttcat tatatgaagt tgaaacaaca ggaataccta gagcatctat
     2521 atctccattt tcatctgtaa tacacacttt gtcaaaatat atattaaaac tatcggcacg
     2581 cacctcattt attaacaaaa caatatcatc gaccttaagc tcattgcttg ttctatataa
     2641 aatagttgga agcctgacac cataatcaat aaagaactga cttcttatcc tttcaataag
     2701 accttccata tcatttgcat ttatcttatt ttcggcaaat aataaaatta aaggaacggt
     2761 ttctgaacta atagcatcca gattttctat cattgccaaa gatgagtcat ggctattatc
     2821 aatatcaaat gtaccagtat agccactaga atcagactca gacagacttt tttctttttc
     2881 tacgaccttt ttataataga ataaagccgt caaagtaact gctatcagga aaaaaacaaa
     2941 aaacggaaaa cctggcaaca tccctatcgc caaggcaaga gctgatgtaa caatgagaac
     3001 aaaaggattc ccaaatatct gggacattat gttacgtccc atattatcgc tatcaccatt
     3061 aactcttgta acgataaatc ctgcacttat agaaattaac agagcaggaa tctgagagac
     3121 caatccgtca ccaatagtta gtatggtata agtagataat gcaccagata gggacattcc
     3181 atgttgactc ataccaacag aaatacctcc tattaaattg acaaaaataa taatgatgcc
     3241 agcaattgca tcacctttaa tgaacttcat tgctccatca aatgaaccat acaattgact
     3301 ctcacgttcc agaatactgc gtctttcttt agctcctgcg gcatcaataa ttccagcctt
     3361 taaatccgca tcaatgctca tttgtttccc cggcataccg tcaagagaaa agcgagcagc
     3421 tacctccgca accctttcag atccttttgt tataacaatg aactgcacaa cagttactat
     3481 tgaaaaaata acaaaaccta cagccagact atcaccaatg acaaactggc caaatgtagt
     3541 aataatttta cccgcatccg catcaactaa aattagtcga ctagtactta ttgataaagc
     3601 gagacgaaat aatgtcgtta tcaacaaaac cgaagggaat gtagaaaaac tcaatattct
     3661 ttcaatataa aatgatccca taaaaacgag aatagcaagt acaatattaa gccctattaa
     3721 aaaatcaaca aggtatgtag gtaatggtat gatcaacata gcaatgatca taaccataag
     3781 cactaatata attaattcag gcttagtact tacttgctta agaaaagact ggatcacttt
     3841 tatctcctgt tatctagaaa gctctttctt gtatgcactt gtgacaagct ctcgtagaat
     3901 attgatcgca atttctcttt cactttcact tttaaatata tcatgaggac tttcattaac
     3961 agaagataga tatgtttgat acactacaga atcctcatga ctggctcgtg atacttctat
     4021 aacagaagta agaatttgct cactttcact tggatattga aaaatatata ataaagttag
     4081 caatagctgc tctgcactta aactgccatc ttttagcaag cttgaggatt ccagtttctt
     4141 cataaaaatt acgtctgaag ttctaatcat cttaattgca attaatttag ataacacaaa
     4201 accaaactca taagcattac agctcggttt ctcagaatcc atgtctacaa tcagtgactg
     4261 ctctacaaaa ttcacaattg tatgtctgtg attaaaacca aaattaccaa gccattctat
     4321 atactgatct gttgttgata tgttccccat gataaaacca cgataacatg cacgcaaaag
     4381 ctgtggttca agtttcattt tgttgccaaa taatttagcc tgtatagctg aatttattcc
     4441 agcattaaca cttttttcac tacctgatat tatctcatta ataattttag ttaataattc
     4501 gacttgttca gcagtaagat ctttttcatt caaaagttcc cttaatgcta agacttgatc
     4561 acttggatct ttaaaatatc tctttagtct gtctataaaa tcccgatcta gaggaaggtt
     4621 atcttttaac gttctcttta aatcaaaaat cttgtgatta atttcatctt cttccccatc
     4681 taaaatacgt tcctgactat cactatttgt tcctttcagt ttctcaaggt ctcttctgtt
     4741 tataaatgac gaaagagcgg aagccatctc atcagatgag tcaatatact tgctattagc
     4801 caagccagaa tcaagctctg catcagcagt ctcatctcca tcatctgaat ttgtcattgc
     4861 attcaactta tcaatgaatg cagagctaaa aactcctgta tttttaacat caagcataca
     4921 tcacctactt tatagttagt aatttaagta tgaaaccaat gatttttcgt cttccagcaa
     4981 agtagtttca gatgggatta tttgtgtagt cttttgtatt tctctttcag atatgaggct
     5041 tttatattct gcagtattat aataacttga ctcctttatc tcacggggtt gaatcagaaa
     5101 aacccttaca atattactga tattacttgt tttatacttg aacacattac cgattacagg
     5161 aatagaggag agaaaaggaa tagatattat ttcgttagag ttcgtttcat gcgtataacc
     5221 acctatcaac aaacttttgc cttgtggaac tctcgctatt gtactgattt tggtccttcc
     5281 aacttctgga aggacagatg tattttcatt attataattg taatttgact gactatttcc
     5341 tgttccatcc tctatcgtta aagacatctc aatttgtccc cgagatgaaa agcgaggtat
     5401 aacatttatc aatgttccat acgtaacatg ctccaacgaa gaattgcgct caccaactaa
     5461 tgacacataa aaagtcctgt tattatcaaa tatagccgga atattctctt gtgtcaaaat
     5521 caccggcctt gaaactacat ttgctttctt tttctgattt aatgccataa ctgaagcaat
     5581 aaacttattg ccgtccaaag tgcttatcga tgctgatgag ctcatattaa atgacgcacc
     5641 aaacgaatca ccaaatgaag ccgttccttg ccaattaaca ccaaggttat ttaattcaga
     5701 tttatcaata tcgattatcc ataatgataa ttctatatgt cgtttggcta tatctaactg
     5761 agtgataata tcgcgaataa tctgtatttg ctgatcatta cctttgacta atatagaatt
     5821 ggtttctgga tatgcaataa gtgatacatc ctctaatata gaactattgg taactgaact
     5881 aaatgaaaag tcattactat cctcagatac tttctgtgta atattaaagg gtggcatcgg
     5941 atcattttgg gcttgtctgt tactcagagc cttaccatta tttaataaac gttctacaac
     6001 agtagccacc cctgggatga caatatcctc ccctctcata ttataagttc tgtcactaac
     6061 aaatgtattt tttaatttta taaccccaaa attaacttta tctgtcccaa tagaagaaac
     6121 ctgcttatca agcaaagttg ctgtattagc taccagttca accaatgccg gaggaccaga
     6181 tatatagaat gttttatcac taatgtttcc tcttatagga tatctgtgat cataaaggtt
     6241 agcatccttc agatactgaa taagataatt aagtgatata ttctccagca aaataacttt
     6301 gcttattaat tctccagagt catatatgta taatgcattt ccatctttat accatatgag
     6361 tcccacaagt aatgtcagtt tttcaagcat ttcttctgga ttagataaat caaactcccc
     6421 agatattctc ttttttgcag cttgtttgct aacaacaata ggataattta ataacgcaga
     6481 aaaacgttca aaaaaagatc ctaccgtatc actttgagca acatatttcg ctatgtcatt
     6541 ttgttctaat aaatgagagt caatattatt agcattaact atcaatggta acaatacaat
     6601 caagagagtc aaagatttaa tattaaattt tttcatttat ttttttcact aaaaaagtaa
     6661 tattcgataa ttctcttgca gaaaagccta gtcgtgtttt tatctcattt gaaaaatggg
     6721 atgttgatgc atacccatta ttcatggcag ctgaagtgat tgtttgattg tgcaaaaata
     6781 catctaataa accatttact aaccgccaag tgttcagttg ttctttgact tttgccccta
     6841 aagcttttct acataaagat ctaaaataag cctccgacac accataatgc tctgtcagac
     6901 tctttattct tacctccttt tctttctcaa tagttctcag aagataaaga accaaatgat
     6961 atgactcagt cattcgtatc atagccagtg cattacttac attctggtca gatgaaaaga
     7021 tgaatcttaa cattaactta tcaagcatcg attcggtatt acaactatcc cgaagatcat
     7081 tctcatgata gaaatgaata catttttcag catggctatg ctctactaga gcaccactga
     7141 aatagtccca aaaagcaaga aattttgcac aactattaat gttaaagcga taatgatagt
     7201 tcacattatc actgaatttt atattttgat ttcggacaaa aaaaaaccct tccatgctgg
     7261 agccagctca attatgttac gttctttatc ttttacaagc tcaacgttaa ctggttcatg
     7321 tccactagag taaatgtaaa acatattact tgtatttaga cctttatatt tactcacaat
     7381 acaattatat ccctcgaatt aattttaatt taacttcccc atccttctct ataaggatgg
     7441 tatcatttat cttattacct tttaaaacaa ccccgtcttt cagcatagaa aatagtttat
     7501 ctatagctat cttttgtgca tccatgaagc acccatctac ttccttaaca ttaaagttgc
     7561 ccatgaattt acctgcatta aaactataaa ccgccttacc actaatacat ttagagtaaa
     7621 catttatact tttattggtt gtattaaacg accataataa atcatttgga atagaaaaat
     7681 aatccttact tacaggaaca atatgccatt ctttttcaga attagatgat gactttaaag
     7741 cacacccact cagtgttaat agcaatatac ttaaaataaa tattttcaac ttattactac
     7801 catgtcgaat catctgcctc tctccctgct tcaaagacac taattaatga atcgaaggtt
     7861 gtagtcttaa tatagaaatc aatattgtta tatatggaca atgatgcata aaatccctca
     7921 tgcaaatcga catattgatt tgcatctatg agtttattaa gaagtgaact tttttgtgca
     7981 gctgtaagat aaggaaaacg tgaattgaat aattgttcaa tcttattgtg cagttcatca
     8041 ttatctatgg aatttaattc actctgtgag aaatattgca ttatttcata gattatatcc
     8101 tcatcaaatg attgcgaaga tgataatctc tcatttaaat taacaacttc ttcactattt
     8161 aatctttgct gcagagcatt gcttgcattt atttgattaa tcattaacag gattctcttt
     8221 taacaagtct tctgcaattt gtctcagttt cttatgtgct aactctttga aatatttttt
     8281 atcatttttt gttaattttt ccgcaagttc tattttaaca cctgatatta catcctgagg
     8341 actaaactcc actacttgat taccactaca gaatatataa ttattccctt cagaatattt
     8401 taaaataatc ttgacatctg agcgatattt atggagatca agttctaact ttttcctcag
     8461 tgctaaatta cacttgggca aaaccacttt taattctgtt actgtattac gcaacgaact
     8521 aagccactgc tccaataaag tgctaatgat cctctcatca tgtaaatttt ctgaaagcaa
     8581 actagttata tagtcctcaa tatttcttct attttcattt tgctttaact tccattcaca
     8641 aatatagtca ataatatgct caaaagcagt ctgtatacca atttgataac catcacatac
     8701 agcatctata cgcagtgatt cagctttttt cgtagcctcc tttataacac aaattgcttt
     8761 cttcttactc tcagtgtctc tatcttttat ggttttacat aattcctttt caattctctt
     8821 tataacaaca ccatcatagg catgatgtct ggataccggt aaagtgcctt tttgcatatt
     8881 gcataccttc atttaatcgg gacaaatttc tttctacagg cgtataattt ccctccaaag
     8941 caattggatt aaataataag tttattcttt catatatacc aacagatata ttttccttcc
     9001 acgcaataag atgccaaaaa ccacttcgaa tagcggcctc cccttgctta ctgattttgc
     9061 ccccataata actaataacc atctcactat gattaattaa ccgagactct tgactaccga
     9121 taaaataagc aacagctgga agtaaattcc agttggcaac aaacatagcg gttaccccgc
     9181 ttaaaggttc aatatcgcaa tttagatcat atttattaat tatgagatta ttaatctcac
     9241 ttctgattac gccattttct aacagttcag aaggcaaatt caaccgattt ctatttatat
     9301 agaacgctgg atcaaaaatt attgaaagat attttttata aattccatcc attcttatca
     9361 tattttgttt ctcttgaacc accctgtttt gaatgcccat accaaaagaa tgacaactag
     9421 tacagccatc ccgagaaata aatatattac ttcatttgtt aaaaattccg atttaacttc
     9481 cttaacaggt tgtacatttg tataaacata ttcttctttc ggagttaata tgacagatat
     9541 attttcatac ttaacatcag aaaaggtgtt tttcaaaaat cgcttaatat tactaactaa
     9601 tagttcagac tctttcggtg agtcatatat agcgattact gatatatgca tcggttttga
     9661 agatatattt ttttcttcaa gatcatagct aacatgtatt tttgccgaaa taacaccacc
     9721 aatagaaact aaagactgtt ccagccgttg ctcaatagca ctatataaac gggccttttc
     9781 agctcttgga gaagacacta atgaatctgt aggaaacatt tgtgagatat caactctctc
     9841 cggatttggc aaatcgtaca tgcgcatcaa atcaactgcc gatgcaaatg tccccttttc
     9901 gacttgtacc gagatcccct gtttacctcc atcaactttt ctagcagtaa tattatggcg
     9961 ttctagcaca gatattattt catttgcctg tctttgagat aaattagaaa ttaactcttc
    10021 acgttgctca catccaatca acatcagcaa caagaataaa ataaaacctt tatacctaat
    10081 catataaatt aagactttaa taaagtttca acagccgaaa ctgtttttct tgctaacgtg
    10141 ccagctaatg atactcctat tgaataattt gagagcgtcg tctgtaactt ggccaaagat
    10201 tctgggttta atgattctgg attgcttacc atctccatta tttgtgatac ttgaatatct
    10261 gtatccatct taatatcact atatttagcc gacacgacgt cttccagact tgatatctct
    10321 tgagattgaa aatcactggc tttgataata tcaacctgat tgactggata aatgtaattc
    10381 atgaatgctc cctattatct gaagttttga ataattgcag catcaacatc cttaatcact
    10441 ttcactgtat tggattgcgc gttcctatat aatgtatatt cagataattt actttggtat
    10501 tcagccagca actgtggatt cgaaggattt ttagctaatt tatctagtgc caatgttagt
    10561 tcaccttgta atgtttgagt tccatcatca aaagtttcag ataatgaact caatgtccaa
    10621 tcatcattcg gtactgtaac actcatttta tcctcactta tttttatcta aaaaaaacca
    10681 gtgtttatca ttcaacatta cataactgtc tttgctgtta agatatgatt tacctttaaa
    10741 atcatcatct atcaaaagta cagaaaactc aatatactga tctccatata tatttttatg
    10801 ttcagaaatc agtctattaa tgagctcaag cttttcatct gtcagttctt ctctgacaga
    10861 atatgtaact ttattatttt cacaaatctc tttatactgg acatttacct ttgtgaaaat
    10921 acctttagca tccccccttg cattatgatc gctaagatag ttgaatttaa tatttttgat
    10981 atacggaaat tcaacaatca gagcattttt caatttatca agttcggtat cttttgaact
    11041 atttctttct ttacttaaaa acaccagtaa ttccgcttta tcactcacta aattcaacac
    11101 atacaaattg ataaatggga atcgttgatt gatatattgt tgaattctat ttatttcttt
    11161 gtttgacacc agaatatatc tgcatttggt aaatccggtt ttatttaagg ctacagacgc
    11221 ccataccgca tcattttgtg tttcggcaag aatataattg cactgtccag tgttgactat
    11281 tccatatctc ttatcatcta taatttcagc aatttctttt acttcatctt tctttaaaga
    11341 gaggaaaaaa attataatta acacaacaat aacagcaaag aaaaaaaaca ccgagtggtt
    11401 cttatacatt ccgtttagta tatggcctct cgatttatcc tccctcatat ttttaaggtg
    11461 aaaagaaatc ccgtcatatt caaatgaatt aaattgtgaa ttgatatcaa tttgctcacc
    11521 tttttcatca ctaaattgac agatattatc tttgcttatg gtcaaataaa ttataccatc
    11581 cgatttacta tctgttacag gaataatatt ttctgggaaa ttgtcgtttc caaactcaag
    11641 tgtttctatc gtccgtccga gaacaattaa gttattccca taatataacg gaaactcatc
    11701 cccgacaccg tttgtgagtt tcactaataa cctgaatggt gcaagatttg agttctttgc
    11761 ctcagacata aatcctctta tatccttcga ttattctgct tgctctctgc tgctatccgc
    11821 aaatagcgtc tatagatatc ctctgcgtac tttaatcttt cttttttctt tttcggagac
    11881 gttcctgcat tataggctcc aacagcctcc caccctcttc catacatcat cataaactca
    11941 tttaataact ttgctgcata atgtacagaa agacatggat gactaattag catttcctca
    12001 gaatatccca tttctctaag tcttttagaa tgaaagtcat ttatttgcat tataccataa
    12061 tctttacttc cattgttatt aacattgact gcagatttat taaatccgga ttctttttcc
    12121 gcaatcgctt ttaacaaact tgatggaata ttgtaccttt caccagcctt atcccaacaa
    12181 tcagctctcc ctaaatatgg gataaaacac agcaaaataa atacgaaacg ggacattaat
    12241 accccttcat tcttcgcgca aattcatcct tcagccaacg cacacgcgaa atataatatt
    12301 ctgtattaac atatacatcc tcgaattcat ttatcccggt aagatctaat cgcgcaatta
    12361 gatttccgcc ttcatcatca gtagctaagc atatcttttc tgagtaattt aggctcaacg
    12421 catatataag atcatttata ttttcaggca atgcacaaaa agggcataat aaatttattg
    12481 aatctccttc tttttcaata tatattagca catcatcatc aagcatgatt gcttgatcat
    12541 cgatatcaat taaagggata cccaaggctc ggcaaataac atctgctaaa tcttccatat
    12601 attcctctta tacaaatgac gaataccctt tcaccatatt ccatattttt gggtccccta
    12661 ttctttcaga ataagatagc tctaacgaag atagcggcaa ttttttcatg actttatttc
    12721 cgggcacacc agtattcatc tcttggattt ccatattacc actattcatt agaatagtag
    12781 aaaataatct tttctcttct gaggataatt tactatttaa ttgagaaaac tgacctgttt
    12841 catgttttct tattatttct cttttaattt cagcatcctg catacctgtt ctgtccttcc
    12901 cactcttaca attccagcaa ggtacagcac ctatagtata agccaataga gtcattcttt
    12961 gtgacaattt atacggctct ccattatcat ttttttgtaa ctttttggtt acaatctctt
    13021 ttatctggtt agccagatat atcacatcat tcttacatgg tggatttttt tctattgctt
    13081 ctgcagccca acctccaatc acaccatttt tgaggaaatt atcccccaac aaagaacata
    13141 ttgattcatc attaagcttg tcaacattcc tccagcctaa gcccattttt agcgctaatt
    13201 catttacacc aaagttaaat gtcaccactt ttagattaac gcttacttct ttcagaagac
    13261 catcactatt ccgaattaat aatttcgttg gcttccccct tttagagttt aaccctttta
    13321 atgcgttcac ctgatctttt aacatactct cctctccccc ggttaaactg gtcggcgtca
    13381 gaagagaagt cgaaacaatc tttaaatcta ctgttttacc agacaaagcc tgtgataata
    13441 actctggtct gctatataat gctgcgctta ctaactcctc agctttatta cgagcagcaa
    13501 cagctctttc agatgagttt tttttcaatc cataggctga tataactcca tgtctgatac
    13561 ccgaaaaaat ttccttcccc tcatcatcca ccacttttga aagccacata ttcgcgatat
    13621 gatcactctc tctcgtagaa gcacaacaaa ttccttttcc attatactct ttgacaaata
    13681 tatttttatt ccctatcttc atatgagagg caggagttaa ttgaaaacca taatttttcc
    13741 catgatgact aatattcttg tcgaccggtc cccaattctt attatttaaa taatcagtaa
    13801 atatttgttt tattttcgtt tgcaattcct ttttattttt aatgcctagc tgtgctctta
    13861 tctcttcact tattacttta ttaagctcat taatatgtct tttattaact tcaccaagtt
    13921 tataggtatc taatcgttcc atcatggctt taatttttga cgtatcacca cctgccaact
    13981 ttattgcctc gcaaaaaact tcagctctgt cacgcgcaaa caaaaggctt tttcctgtta
    14041 agcttgtttg gttctgtaaa tataatcgat taagcgcttc gatcccctta tttctttctc
    14101 catttttaac tctttgatat gaaatcacgc ttacaccttt gtgctttccg gtttcttctg
    14161 cgcctttata ggaatctccg ctttgaaatg aaacctgatg caatcccaaa ttagttatgt
    14221 gcatttaatt atcctcagtc ataaatgggg cattgattgc agttttgtac ctcgttttac
    14281 aacaaaaaag atacttattc aacagggttc tctctttttc tataacactc cgagcctgat
    14341 acatcaggca ctttatcttg tgggatttca tgatgaaacg agcactacat atgctcacga
    14401 ggtacatatt atgtacagca aaagaaaatt tgattatgtt aacaggctca agtttctaaa
    14461 tttattaatt gaatatatta tataccaaat acattttaca tccccacaat caccaagtaa
    14521 tggagagtta ataaagtatg atcctcaaaa ttagcaattt cattgacgca agcaatacaa
    14581 aagggcctat acgcgttgaa gatacagagc atggacctat attgatagca cagaaattca
    14641 acctaaagga tctctttttc agaacattaa gcactattaa tgcaaagata aattcacaga
    14701 tattaaatga acaactaaaa aattatcgac tagagaatca aaaatcctta ttgcttttcc
    14761 tcaatacatt agctagcgaa aaatctgcag aaagtgcgtt tgccgcctat gaagcagcta
    14821 aaaactcaat tcaacactct ttcacaggta gagacatcaa attaatgttg aataccgcag
    14881 agcgttttca tggcatcggt acagccaaaa atcttgaaag acatttagtt tttcgttgct
    14941 ggggaaatag aggcataacc catttagggc atactagtat cagtataaaa aacaatttac
    15001 tacaggaacc aactcatacc tatctcagct ggtatcccgg cgggaatgtt acaaaagata
    15061 cagaaataaa ctaccttttt gaaaaacgct caggttacag tgtcgatacc tataagcaag
    15121 acaaactaaa tatgatttca gaccaaacag ccgaaagact tgatgcaggc caagaggtta
    15181 gaaacttatt aaattcaaaa caagatcaaa ataacaacaa aaaaatattt tttcccagag
    15241 ctaatcaaaa aaaagacccc tatggttatt ggggggtcag tgctgataaa gtatacattc
    15301 cattatcagg tgataataaa acaaaggatg gtaaaatcag ccataacctt tttggccttg
    15361 atgaaacaaa catgtcaaaa tttatatgca aaaagaaagc agatgctttc agacaattgg
    15421 caaattataa attaatcagt aaatctgaaa actgtgctgg tatggcactt aatgttctta
    15481 aagcaggaaa ttctgaaata tactttccac tccctgacgt taaattggtt gctacaccta
    15541 acgatgtata cgcgtatgca aataaagtta ggcaacgcat tgaaagtctc aatcagtctt
    15601 ataacgaaat aatgaagtat attgaatctg attttgatct ttccagattg actcaattac
    15661 gacgcagcta tcttaaaagt ttcaataaaa ttaaccttat ccacacacca aaaacattca
    15721 agcctttatc aatttcactt tacaaacatc ctactgaaaa tgtatcttca gaagactttg
    15781 atgcggtcat caacgcttgc cactcatact tagtaaaatc agcacccagc aatatgactc
    15841 gtgtattaaa cgaattaaaa accgaagcaa cagataaaaa agaagaaatc atcgaaaaat
    15901 caataaaaat tattgattat tacaattctc ttaaatcgcc agatcttgga accaaacttt
    15961 acattcacga cttattacag atcaataagc ttttattgaa taactctcat tctaatatat
    16021 agaaggccat agaaatgtgt cgcaaactat atgataaact ttatgaaata acaggtgcaa
    16081 agcttgattt taacgataaa aatcaagcct ttatattgct cgaagaacaa atacctgttt
    16141 gtataacaga taatgatgaa tacatctttt tgaccggact tcttaatgaa catgaacttt
    16201 ttacagaaaa tattattaat ccagaacaca tactcatcct taactactct ttgagtagag
    16261 attatggttc ttctatctgt cttctccctg atactcatca atgtgttttg accaagaaac
    16321 actacaaaaa atatctttca cctgatgaac tcattgaatc tttgtatgaa tttttatttt
    16381 gtattaaact aaccattgca aacatcactt cagaagtgaa ctaacatata gggggtatca
    16441 tgcaaattct aaacaaaata cttccacagg ttgaattcgc gattcctcgc ccatcatttg
    16501 attcgttaag tcgcaataag cttgtaaaga aaatattatc tgtttttaac ttaaaacaac
    16561 gctttcctca gaagaatttt ggatgcccgg tgaatataaa taaaatcagg gacagcgtca
    16621 tagacaaaat aaaagatagt aattcgggga atcaactatt ttgttggatg agccaagagc
    16681 gaaccactta tgtctcttca atgataaaca gaagtataga tgaaatggcc atacacaatg
    16741 gagttgtttt gacctcagat aacaaaaaaa acatatttgc agctattgaa aaaaaattcc
    16801 cggatataaa acttgatgaa aaatcagcac aaacatctat cagtcataca gcactaaacg
    16861 agattgcctc atctggcctc agagcaaaga ttttaaaacg ctatagcagt gacatggatt
    16921 tgtttaacac acaaatgaag gatcttacaa atctagtatc cagttccgtc tacgataaaa
    16981 tatttaatga atcaacaaaa gttttacaaa tagaaataag cgcagaggtt ctaaaggctg
    17041 tataccgtca aagcaataca aattaaatct tttatattaa atcttatact tattaaagta
    17101 aagcagacaa aaaggagacc ttatgtcttt aaatatcacc gaaaatgaaa gcatctctac
    17161 tgcagtaatt gatgcaatta actctggcgc tacactgaaa gatattaatg caattcctga
    17221 tgatatgatg gatgacattt attcatatgc ttatgacttt tacaacaaag gaagaataga
    17281 ggaagctgaa gttttcttca ggtttttatg tatatacgac ttttacaatg tagactacat
    17341 tatgggactc gcagctattt atcagataaa agaacagttc caacaagcag cagaccttta
    17401 tgctgtcgct tttgcattag gaaaaaatga ctatacacca gtattccata ctggacaatg
    17461 ccagcttcgg ttgaaagccc ccttaaaagc taaagagtgc ttcgaactcg taattcaaca
    17521 cagcaatgat gaaaaattaa aaataaaagc acaatcatac ttggacgcaa ttcaggatat
    17581 caaggagtaa ttattatgca taatgtaagc accacaacca ctggttttcc tcttgccaaa
    17641 atattggctt ccactgagct tggagacaat actatccaag ctgcaaatga tgcagctaac
    17701 aaattatttt ctcttacaat tgctgatctt actgctaacc aaaatattaa tacaactaat
    17761 gcacactcaa cttcaaatat attaatccct gaacttaaag caccaaagtc attaaatgca
    17821 agttcccaac taacgctttt aattggaaac cttattcaaa tactcggtga aaaatcttta
    17881 actgcattaa caaataaaat tactgcttgg aagtcccagc aacaggcaag acagcaaaaa
    17941 aacctagaat tctccgataa aattaacact cttctatctg aaactgaagg actaaccaga
    18001 gactatgaaa aacaaattaa taaactaaaa aacgcagatt ctaaaataaa agacctagaa
    18061 aataaaatta accaaattca aacaagatta tccgaactcg acccagagtc accagaaaag
    18121 aaaaaattaa gccgggaaga aatacaactc actatcaaaa aagacgcagc agttaaagac
    18181 aggacattga ttgagcagaa aaccctgtca attcatagca aacttacaga taaatcaatg
    18241 caactcgaaa aagaaataga ctctttttct gcattttcaa acacagcatc tgctgaacag
    18301 ctatcaaccc agcagaaatc attaaccgga cttgccagtg ttactcaatt gatggcaacc
    18361 tttattcaac tagttggaaa aaataatgaa gaatctttaa aaaatgatct ggctctattc
    18421 cagtctctcc aagaatcaag aaaaactgaa atggagagaa aatctgatga gtatgctgct
    18481 gaagtacgta aagcagaaga actcaacaga gtaatgggtt gtgttgggaa aatacttggg
    18541 gcacttttaa ctatcgttag tgttgttgca gcagcttttt ctggaggagc ctctctagca
    18601 ctggcagctg ttggtttagc tcttatggtt acggatgcta tagtacaagc agcgaccggc
    18661 aattccttca tggaacaagc cctgaatccg atcatgaaag cagtcattga acccttaatc
    18721 aaactccttt cagatgcatt tacaaaaatg ctcgaaggct tgggcgtcga ctcgaaaaaa
    18781 gccaaaatga ttggctctat tctgggggca atcgcaggcg ctcttgtcct agttgcagca
    18841 gtcgttctcg tagccactgt tggtaaacag gcagcagcaa aacttgcaga aaatattggc
    18901 aaaataatag gtaaaaccct cacagacctt ataccaaagt ttctcaagaa tttttcttct
    18961 caactggacg atttaatcac taatgctgtt gccagattaa ataaatttct tggtgcagcg
    19021 ggtgatgaag taatatccaa acaaattatt tccacccatt taaaccaagc agttttatta
    19081 ggagaaagtg ttaactctgc cacacaagcg ggaggaagtg tcgcttctgc tgttttccag
    19141 aacagcgcgt cgacaaatct agcagacctg acattatcga aatatcaagt tgaacaactg
    19201 tcaaaatata tcagtgaagc aatagaaaaa ttcggccaat tgcaggaagt aattgcagat
    19261 ctattagcct caatgtccaa ctctcaggct aatagaactg atgttgcaaa agcaattttg
    19321 caacaaacta ctgcttgata caaataagga gaatgttatg gaaattcaaa acacaaaacc
    19381 aacccagatt ttatatacag atatatccac aaaacaaact caaagttctt ccgaaacaca
    19441 aaaatcacaa aattatcagc agattgcagc gcatattcca cttaatgtcg gtaaaaatcc
    19501 cgtattaaca accacattaa atgatgatca acttttaaag ttatcagagc aggttcagca
    19561 tgattcagaa atcattgctc gccttactga caaaaagatg aaagatcttt cagagatgag
    19621 tcacaccctt actccagaga acactctgga tatttccagt ctttcttcta atgctgtttc
    19681 tttaattatt agtgtagccg ttctactttc tgctctccgc actgcagaaa ctaaattggg
    19741 ctctcaattg tcattgattg cgttcgatgc tacaaaatca gctgcagaga acattgttcg
    19801 gcaaggcctg gcagccctat catcaagcat tactggagca gtcacacaag taggtataac
    19861 gggtatcggt gccaaaaaaa cgcattcagg gattagcgac caaaaaggag ccttaagaaa
    19921 gaaccttgcc actgctcaat ctcttgaaaa agagcttgca ggttctaaat tagggttaaa
    19981 taaacaaata gatacaaata tcacctcacc acaaactaac tctagcacaa aatttttagg
    20041 taaaaataaa ctggcgccag ataatatatc cctgtcaact gaacataaaa cttctcttag
    20101 ttctcccgat atttctttgc aggataaaat tgacacccag agaagaactt acgagctcaa
    20161 taccctttct gcgcagcaaa aacaaaacat tggccgtgca acaatggaaa catcagccgt
    20221 tgctggtaat atatccacat caggagggcg ttatgcatct gctcttgaag aagaagaaca
    20281 actaatcagt caggccagca gtaaacaagc agaggaagca tcccaagtat ctaaagaagc
    20341 atcccaagcg acaaatcaat taatacaaaa attattgaat ataattgaca gcatcaacca
    20401 atcaaagaat tcgacagcca gtcagattgc tggtaacatt cgagcttaat atatccaaga
    20461 gccataataa tatatggctc ttcctgtaag gaaataacca tgaatataac aactctgact
    20521 aatagtattt ccacctcatc attcagtcca aacaatacca acggttcatc aaccgaaaca
    20581 gttaattctg atataaaaac aacgaccagt tctcatcctg taagttccct tactatgctc
    20641 aacgacaccc ttcataatat cagaacaaca aatcaggcat taaagaaaga gctttcacaa
    20701 aaaacgttga ctaaaacatc gctagaagaa atagcattac attcatctca gattagcatg
    20761 gatgtaaata aatccgctca actattagat attctttcca ggcacgaata tccaattaat
    20821 aaagacgcaa gagaattatt acattcagct ccgaaagaag ccgagcttga tggagatcaa
    20881 atgatatctc atagagaact gtgggctaaa attgcaaact ccatcaatga tattaatgaa
    20941 cagtatctga aagtatatga acatgccgtt agttcatata ctcaaatgta tcaagatttt
    21001 agcgctgttc tttccagtct tgccggctgg atctctcccg gaggtaacga cggaaactcc
    21061 gtgaaattac aagtcaactc gcttaaaaag gcattggaag aactcaagga aaaatataaa
    21121 gataaaccgc tatatccagc aaataatact gttagtcagg aacaagcaaa taaatggctt
    21181 acagaattag gtggaacaat cggcaaggta tctcaaaaaa acgggggata tgttgtcagt
    21241 ataaacatga ccccaataga caatatgtta aaaagcttag ataatctagg tggaaatggc
    21301 gaggttgtgc tagataatgc aaaatatcag gcatggaatg ccggattctc tgccgaagat
    21361 gaaacaatga aaaataatct tcaaacttta gttcaaaaat acagtaatgc caatagtatt
    21421 tttgataatt tagtaaaggt tttgagtagt acaataagct catgtacaga tacagataaa
    21481 ctttttctcc atttctgagg tgcgccatgc ataatgtaaa taatactcaa gcgccaacat
    21541 tcttatataa ggcaacttca ccatcatcaa cagaatacag cgagttaaaa agcaaaatat
    21601 ccgatatcca tagttcgcaa acttctctaa aaacaccagc atcagtgtct gaaaaagaaa
    21661 actttgcaac gtcttttaat cagaaatgtc ttgatttttt attttcttcc tcagggaaag
    21721 aagatgtgtt aagaagcatt tattccaact caatgaatgc gtatgccaaa agcgagattc
    21781 tcgaattttc aaatgttttg tactccttag tacatcaaaa tggtcttaat tttgaaaacg
    21841 aaaagggact tcaaaaaatt gtcgcacagt attcggaact aattataaaa gataaattat
    21901 cccaagattc tgcctttgga ccatggtcgg caaagaataa gaaactccat caattacgac
    21961 aaaacattga gcacagactt gcactattag cacaacaaca cacatctggt gaagctttat
    22021 cattgggaca aaaactcctc aatactgaag tatcatcatt tatcaagaat aatattcttg
    22081 ctgaattaaa gttaagtaat gaaactgttt catctctcaa actagatgat ttagttgacg
    22141 cacaggcaaa acttgccttt gatagtttgc gcaatcaacg taaaaatact attgatagta
    22201 aaggatttgg tataggtaaa ctgtcaagag acttaaatac agtagccgtg tttcctgagc
    22261 tgttgagaaa agtccttaat gatattttag aagatataaa agattcgcat cctatccaag
    22321 atggcctccc tacacctccc gaagatatgc cagatggcgg accaaccccc ggagccaatg
    22381 agaaaacatc ccaacctgta attcactatc atataaataa tgataataga acttacgata
    22441 atagagtttt tgacaacaga gtatatgaca atagctatca cgagaaccca gaaaatgatg
    22501 cacagtctcc tacttctcag acaaacgatc tattatcccg taacggaaac tcattactaa
    22561 atccacaaag agcactagtt caaaaagtaa cttccgttct accacactct atatcagata
    22621 ctgtccagac atttgcaaat aattcagctt tagaaaaggc tttcaaccat actccagata
    22681 attcggatgg aataggttca gacctgttaa ctacgagtag tcaagaaaga tctgcaaata
    22741 actctctttc tcggggacac aggcctctga acatacagaa ctcttcaacc accccccctc
    22801 tccacccgga aggagtgaca agcagtaatg ataactcatc agatacaact aaaagtagcg
    22861 cttctctttc tcatagagta gcttcgcaaa tcaataaatt caactcaaac actgattcaa
    22921 aagtacttca gactgatttt ttatcaagaa atggagacac atatttaaca cgggaaacga
    22981 tatttgaagc ttcaaaaaaa gtaacaaact ccctaagtaa tcttatatct ctcattggaa
    23041 ctaaatcagg aacacaagaa cgagagttac aggaaaaatc aaaggacatt acaaaatcca
    23101 caacagaaca tagaataaac aacaaattaa aagttacaga tgcaaatata agaaactacg
    23161 taacagaaac caacgcagat acaattgata aaaatcatgc gatctatgaa aaggcaaaag
    23221 aagtatctag cgccctcagc aaggtattgt caaaaattga cgatacctct gcagaattac
    23281 ttacagatga tatatctgat ttaaaaaata acaatgatat tacagctgaa aacaataata
    23341 tatataaagc agcaaaagat gtaaccactt ccctatcaaa agtattaaag aatatcaata
    23401 aggattaaaa tatgataaaa gaaaaaatat tatcaatagt ggccttttgc tatggtatag
    23461 cgtacagcaa actatctgaa gaaactaaat ttattgaaga tctttctgcc gattctcttt
    23521 ctctgattga aatgctggat atgattagtt ttgagttcaa tctacgtata gatgaatcta
    23581 cattagaaca cattattact attggagatc ttatttctgt agtcaaaaat agtacaaaat
    23641 caatatagta attgataagc attttttcat ctatggagct ctcacatcag agctccacaa
    23701 gaatattatt cttttatcca ataaagataa attgcatcaa tccagctatt aaaatagtaa
    23761 tttttaagac taccgttgac tatcatcaac agttacatac aatcttgctc acactgcatt
    23821 taacttttgt caatataaat caatatgaat aaacagggtg tgatatggtg gatttgtgca
    23881 acgacttgtt aagtataaag gaaggccaaa agaaagagtt tacactccat tctggtaata
    23941 aagtttcctt tatcaaagcc aagattcctc ataaaaggat ccaagattta accttcgtca
    24001 accaaaaaac gaatgtacgc gatcaagaat ccctaacaga agaatcatta gccgatatca
    24061 taaaaactat aaagctacaa caattcttcc ctgtaatagg aagggagatt gatggtagaa
    24121 ttgaaattct ggatggcact cgtagaagag catctgcaat atatgcagga gcagatcttg
    24181 aagttctata ttcaaaagaa tatatatcta ctcttgatgc cagaaaacta gcaaacgata
    24241 tacaaacagc aaaagagcat agcatccgag aacttggtat tggtcttaat tttctgaaag
    24301 tatcagggat gtcctataaa gacatagcca aaaaagagaa tctgtctcgc gcgaaagtca
    24361 ctcgtgcctt tcaggcagca agcgttccac aggaaataat atctctattt ccaatcgcgt
    24421 cagaacttaa ctttaatgac tacaagatat tattcaatta ttataaagga cttgaaaagg
    24481 ctaatgaatc tcttagttct acactaccaa tattaaagga agaaataaaa gaccttgata
    24541 caaatttgcc cccggacata tataaaaaag aaattttaaa catcataaag aaaagcaaaa
    24601 acagaaagca aaacccttcg ctaaaagttg actcgttatt tatttctaaa gacaaacgga
    24661 cttacataaa aagaaaagag aataaaacaa acagaactct aatatttaca ttatctaaaa
    24721 taaacaaaac agttcagaga gaaatagatg aagctattcg ggatataatt tctcgccatc
    24781 tatcgtcttc ataaacatcg aacactgatg taaactgccc aattcagata ataaaaatct
    24841 tcgttgaact tgactttcag ccggtggggt aattccgtcg gctatctgtt attcaatccc
    24901 cacgcactgt gtttccttaa aattacggtg aaaactcata ccctgctgac cacgagaaaa
    24961 ttatctggtg aaattagtaa tattcatatc attgaaaatg aattgtgcaa ctaagggatg
    25021 cggcatgatg gtcaaaattt tatgtgaata tgagtgactg gccgaaaata ttcagcgtat
    25081 ttttaatcgt ttcaataaaa acagcgagga ttagcagtca acgcttttac tactcaattt
    25141 tttttacatg gtgataaaca aaaaatccac cattaacaga cttcgttcac agtatcaaaa
    25201 aagctttaag aatatattag cagatttctc aaatataatt actgttttat tctcctaatc
    25261 ttcccataaa atgaccgact tacattattt tataattata aacaagtttg attttaaaat
    25321 catattaatt attaacaaga gatttatttt tctaacccca aaagttatgc ttggtcaaag
    25381 agtaacttat aatatccata cccctcatag ggcttacagt ttaaattgcg ggtataaaat
    25441 acatctattt tcaaataaaa taaagcatac tccaattaca acgagtgact aaaataaaaa
    25501 tatcatccaa gtcaatcact gtttgctctg caaaatatgt cagccttctc aaacttacca
    25561 gcacttaaat atttaagcac acaatcattc cttgagggag ttcacttatt tcaagagaaa
    25621 catttcgtct tcccacacaa ctaatccgcc caatatagct aaatttgtac tcagtaatat
    25681 catttgttat gccccccacc ccaaaatatt caagctcctt cacagataaa aaacgacaat
    25741 ttacaaagcc tcattagtta taactatgga aatacctatt ttagtatatc ctataaagtt
    25801 agaattactt cttcttgcac ctaattccat agttgaaaaa caagaataat tttctcctac
    25861 agctggatcc atatagcttc catcaggtct acacaacacc cagtggaggc cgacaggaac
    25921 tccaataaag gatacagcaa cagcttccaa aactctttgg ttagaagata acacatctcg
    25981 ttgatgtact atattacatc ccatgccaat taataagtcc cttgcatcgg gataaataaa
    26041 actaattact tgcgaaaaaa tgttactttc ttcaacgaca taagcattca atccaagaag
    26101 cctcgtagcc atgactatct tatcaggcat agaataaccg gcatccgcga tattatcttt
    26161 gtgtattctc ggattataat taccactagt tattaagtat aaatcccgct cacaacgatt
    26221 atccaaatca agagaactct tccttgtcat ttcggacata gaccctttgt attgtggtat
    26281 tttatcaacc cctaattcct ttgcagcaca gagtaaactg gcagctccac aagaattgtc
    26341 atacatctgc ttcctcgcca aaaaacaacc ttgttcgttt actctattct catttaccct
    26401 cacatcagtc tgatgagata tcatatattc tcttggtata gccccctgca ataaaacccc
    26461 atataacata acacctgtat gaatacatcc ccgtttgcaa ggcttccgtt gttccgacat
    26521 cgacgagtaa ttgcagcaca gtattgcatc cagcaatgcg aactactatg agaattagct
    26581 gaccgggtcc ttattccccg aacgaacttt aaatccaaaa cctcatactc ttttccgtcg
    26641 tcatcacgtc cgtcaccata acaacctttc agcatccacc cttttattaa gagttaatgt
    26701 ccatcagcat cagcaattaa taccgcctga tactgacata tttttgtcgt atttacgtcc
    26761 ataatcggtt attgtgatac aggtcacacc agcttatttg ctattgttac aataaccacc
    26821 ttggttgaga gctgtttttt caactttgtt atcaactccc ccaactcctt ggtcagtaag
    26881 gctgccgacc gagcacagtg aatagatagt atccgcaggt accaccatgc ttacttaata
    26941 ctcagaaggc gaagtgctct ccaattaact ctgccgcact atgcttataa gctgcttttc
    27001 aatgccgtct atcaacatgt ccaattcatt caatctgttg tactggtaat ccaacgcagt
    27061 aatgatatat gcaggtattc tttcatccac tccagtaacg tttcatgcaa agcattaatt
    27121 tacgcgctac ggaacttcac cagttgactg agcaaacgat gtgggaccag tactgcctgc
    27181 agttcttcta ttgttacagc gatttcttta ccaaacagct gaaccaccat caagataaag
    27241 atagcctgaa cattcatcgc accattttgt taccaatggc aaaagattta acgaaccgtg
    27301 cctgcatcag gcggactttt tatccctgct ttgttagctc cctaaccttg tacagagagc
    27361 ctccataagc tcccatatca agcagacatg gttcgcagtt actgacatac accagaaaac
    27421 tctgacagca gactcactta tcctccactt cactcatatc cttattggtg agatgaacct
    27481 gaatgacatg ttttgcgaca taaacgccaa ccagtgtata tttcacctgt agagattcta
    27541 caatctgtag cgttatgcat ctaatgttag gctctatgat gtcagaaatc tgcgagactc
    27601 tacaccttct gcatttacct ttatttatca ccatgctcct cagttaacgg agaacgtgtt
    27661 cattgcattt tgtcacttac atttcgcctt tggtatcggg gggatatagg ttccaacgtt
    27721 ccgcatgaga acctgaaccg gcttcacgcc accttgatac cagatgccgc acaggtagtc
    27781 aacaagtgtc acgaacggtg caatagtgat ccacacccaa cgcctgaaat cagatccagg
    27841 gggtaatctg ctctcctgat tcaggagagc ttatggtcac ttttgagaca gttatggaaa
    27901 ttaaaatcct gcacaagcag ggaatgagta gccggacgat tgccagagaa ctggggctct
    27961 cccgcaatac cgttaaacgt tatttgcagg caaaatctga gccgccaaaa tatacgccgc
    28021 gacctgctgt tgcttcactc ctggatgaat accgggatta tattcgtcaa cgcatcgccg
    28081 atgctcatcc ttacaaaatc ccggcaacgg taatcgctcg
//
LOCUS       DAJTYH010000052        28008 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTYH010000052 DAJTYH010000000
VERSION     DAJTYH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..654
                     /locus_tag="OO609_002169"
                     /pseudo
     CDS             1..654
                     /locus_tag="OO609_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            651..1037
                     /locus_tag="OO609_002170"
     CDS             651..1037
                     /locus_tag="OO609_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633580.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1062..3275
                     /gene="tssI"
                     /locus_tag="OO609_002171"
     CDS             1062..3275
                     /gene="tssI"
                     /locus_tag="OO609_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6633581.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTHDVSE"
     gene            3298..3743
                     /locus_tag="OO609_002172"
                     /pseudo
     CDS             3298..3743
                     /locus_tag="OO609_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3765..4172
                     /locus_tag="OO609_002173"
     CDS             3765..4172
                     /locus_tag="OO609_002173"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6633582.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4169..6457
                     /locus_tag="OO609_002174"
     CDS             4169..6457
                     /locus_tag="OO609_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6633583.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6454..7443
                     /locus_tag="OO609_002175"
     CDS             6454..7443
                     /locus_tag="OO609_002175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633584.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7543..7746
                     /locus_tag="OO609_002176"
     CDS             7543..7746
                     /locus_tag="OO609_002176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633585.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7764..8084
                     /locus_tag="OO609_002177"
     CDS             7764..8084
                     /locus_tag="OO609_002177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633586.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8150..8382)
                     /locus_tag="OO609_002178"
                     /pseudo
     CDS             complement(8150..8382)
                     /locus_tag="OO609_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8492..9262)
                     /gene="yafV"
                     /locus_tag="OO609_002179"
     CDS             complement(8492..9262)
                     /gene="yafV"
                     /locus_tag="OO609_002179"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6633587.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9443..9889
                     /gene="ivy"
                     /locus_tag="OO609_002180"
     CDS             9443..9889
                     /gene="ivy"
                     /locus_tag="OO609_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6633588.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9932..12376)
                     /gene="fadE"
                     /locus_tag="OO609_002181"
     CDS             complement(9932..12376)
                     /gene="fadE"
                     /locus_tag="OO609_002181"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6633589.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12616..13194
                     /gene="lpcA"
                     /locus_tag="OO609_002182"
     CDS             12616..13194
                     /gene="lpcA"
                     /locus_tag="OO609_002182"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6633590.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13400..14167
                     /locus_tag="OO609_002183"
     CDS             13400..14167
                     /locus_tag="OO609_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6633591.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14138..14878)
                     /locus_tag="OO609_002184"
     CDS             complement(14138..14878)
                     /locus_tag="OO609_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6633592.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15262..15783
                     /locus_tag="OO609_002185"
                     /pseudo
     CDS             15262..15783
                     /locus_tag="OO609_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15959..16456
                     /gene="rayT"
                     /locus_tag="OO609_002186"
     CDS             15959..16456
                     /gene="rayT"
                     /locus_tag="OO609_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6633593.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16673..18385)
                     /locus_tag="OO609_002187"
                     /pseudo
     CDS             complement(16673..18385)
                     /locus_tag="OO609_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18357..19142
                     /gene="lafU"
                     /locus_tag="OO609_002188"
     CDS             18357..19142
                     /gene="lafU"
                     /locus_tag="OO609_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6633594.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19213..20268
                     /gene="dinB"
                     /locus_tag="OO609_002189"
     CDS             19213..20268
                     /gene="dinB"
                     /locus_tag="OO609_002189"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6633595.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20320..20613
                     /gene="yafN"
                     /locus_tag="OO609_002190"
     CDS             20320..20613
                     /gene="yafN"
                     /locus_tag="OO609_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6633596.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20616..21014
                     /gene="yafO"
                     /locus_tag="OO609_002191"
     CDS             20616..21014
                     /gene="yafO"
                     /locus_tag="OO609_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6633597.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21024..21476
                     /locus_tag="OO609_002192"
     CDS             21024..21476
                     /locus_tag="OO609_002192"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6633598.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21722..22765
                     /locus_tag="OO609_002193"
     CDS             21722..22765
                     /locus_tag="OO609_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6633599.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22827..23441
                     /locus_tag="OO609_002194"
                     /pseudo
     CDS             22827..23441
                     /locus_tag="OO609_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23498..24955)
                     /gene="pepD"
                     /locus_tag="OO609_002195"
     CDS             complement(23498..24955)
                     /gene="pepD"
                     /locus_tag="OO609_002195"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6633600.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25217..25675
                     /gene="gpt"
                     /locus_tag="OO609_002196"
     CDS             25217..25675
                     /gene="gpt"
                     /locus_tag="OO609_002196"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6633601.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25767..27011
                     /gene="frsA"
                     /locus_tag="OO609_002197"
     CDS             25767..27011
                     /gene="frsA"
                     /locus_tag="OO609_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6633602.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27069..27470
                     /gene="crl"
                     /locus_tag="OO609_002198"
     CDS             27069..27470
                     /gene="crl"
                     /locus_tag="OO609_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6633603.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27509..>28008)
                     /locus_tag="OO609_002199"
     CDS             complement(27509..>28008)
                     /locus_tag="OO609_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6633604.1"
                     /translation="DGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEA
                     WATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVL
                     SKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAVG
                     MTYQF"
BASE COUNT         6916 a         6957 c         7478 g         6657 t
ORIGIN      
        1 gaaaaaacaa atattgatgg caatatcatt atacataagg atggtaggag gacgtattta
       61 ttaaatcaaa atactggttc gattacggct gtgataagat ggaagtatga gtagtataag
      121 gataataccg ttacagatga ctggactata tgggaaaaag ctatttttat gcgagcgata
      181 tatttatctg ttcagcaggc atggaatggg aaaataactt attctgtttc cggtgaatct
      241 gagttcgcta aaaaatttca gggtaaagct ttgccttttg atgtaagaat aatttctgca
      301 tctcagaatg aagactggtt ggttattgca acaaaggttt tgcctggtgc tgatttaaga
      361 acatatgttg attttaaaaa cagtacggtt catgttgatt cagcgggcct ggaaaaagta
      421 gcgaaatgta ttaactgtaa taatacgttg caagtaaata tacctcatga agccggacat
      481 gtacttggtt acttagatga tgattatgat tcttcgtctc cgtatgttgg agatattagt
      541 gggttgatga atgtgggaat ggagctttgg gaacgatacc ttaaaaacgc aactataacg
      601 ttgaatatca taatgcctga aactaaattt actttattga atgtaaccaa atgaaatttt
      661 tctgtattat agtgattttc ctttcaataa attacatagc tatagtgaat gttttcaagt
      721 cttctgcagt tgctcaatcc accaaatatt atgaagaaaa tgatatacat gatggaagta
      781 atgacatctt ttttatgaag aaatatgggc cgcctgctca tgagttcgtg aggccacgat
      841 ataaatttgt tggtgagtat tatatcggct tgaatcaaac ttatcgcata acagatcctg
      901 aatttcaaga tgttcctatt aaggaaatgt tctggaattt gcagggggat ttgaatctga
      961 catgttggtt tcattataaa gacgggcaat ggagagtgat tacttatatt ttttggccac
     1021 caggggctag attttaatcc ggtattaaag gagcaaatat catgagtttt gtatctacgg
     1081 gtaataaacc agttggaaac ggtggacccg tcatcaccac accaccaata gctggcgaaa
     1141 gtggcggtat gagcacaggt tcagctgtaa ccgatgtatc tggtgccgcc gaggagatgg
     1201 cagaacaggc tgcggcggac ctgtttggtg cgctgccgga accgtcaggt ctggttaaag
     1261 ccgcagtagc tgctgcgcag gctgccgctg cagcagcagg gatctcggat atggctggtg
     1321 cagttcagga tgcagcggcg tcgcttgcag ccggtgctcc gggggcgcat aacgtcacag
     1381 taagtggtag tgcggtgccg ccgcagatgc ttttgtttgc aggcatgaat ggttcggaag
     1441 ggcttgggaa tctgttttct tacacagtac agcttaaaac gctggacgcc ctgaacctgg
     1501 ggtacgtttc ccccgctgct aacctgccgc ttcagctcat ggtgggcaaa gacctgtgcg
     1561 tcagtatcga actggacggc ggcggaaaac ggtatatcag cgggctggtg acggcggcga
     1621 gggtggtcgg ccatgagagc cgttcggtaa cgtatgaatt acgcattgag ccatggctga
     1681 agctgctgac ccataccagc gactacaaag cgttccagaa caaaaccgtg gtggatattc
     1741 tcgatgaagt actggatgaa tattcctttc cggtggaaaa gcggctggtg gaaaattatc
     1801 caccccgcgc ctggcaggtg cagtatggtg aaaccgactt cgatttcatc cagcgcctga
     1861 tgcaggaatg ggggatttac tggtggtttg agcacagcga gaacagccac acgctggtgc
     1921 tggtggatgc catcaatggt cataaagcgt gccctgactc gccgctggtg gagtggcacc
     1981 aggaagggct gaaactggat aaggaattta tccacaccat tacggccagt gagaggctgc
     2041 gtactggaaa gtgggtgatg gacgattttg attttatgaa gcctcgttcg ctgcttaaaa
     2101 gtaccgtggc cagtccgcgt gataccggtc atgctgaata cgagcattac gaatggcctg
     2161 gtgattactt cactacgggc gaaggcgaga tgctgacgcg cattcgtatg gaagcgcagc
     2221 gcagcccagg cagccgggca catggggccg gtcatatccg taccctcatg accgggtaca
     2281 cctttacgtt gatgaatcac ccgacggcag aaattaatca ggaatatctg ctggtacaga
     2341 ccactctgtt tttacgggat aatgcgcagc acagcggtca ggatcagcat tttacctatg
     2401 tcaccacgtt tgaactgcac cccaccagcg aagtttaccg cccacaacga accctgagca
     2461 aaccccacac caaaggcccg cagagcgcca tcgtcaccgg cccggcgggg caggaaatct
     2521 ggacggataa atatggccgg gtgaaggtgc agtttggctg ggaccgttac ggaaagaatg
     2581 acgaaaacag ttcctgctgg atccgcgtca gctatccgtg ggcgggtaaa ggattcggca
     2641 tgatccagat cccgcgtatt ggccaggaag tactggtgga tttcaaaaac ggcgatccgg
     2701 atttgcccat catcgtgggg cgcacctata accaggacac catgccgccg tgggggctgc
     2761 cgggcatggc gtcgcagagt gggatattca gccactcatt gcagggaggg ccgactaacg
     2821 gaaatatgct gcgcttcgat gataaaacgg gtgcggaaga ggtgaagttc catgcggaaa
     2881 aagacctcaa taccacggta aaaaacaatg aaacccacac ggtcatggtc gatcgcataa
     2941 aaaccattgt caaaaatgaa accatcagcg tgggagaaga ccgtaatgcc accatcacaa
     3001 aaaatgatgg tctttccgtc aaactggctc aaaccatcaa tgtcggaacg acatatcgtc
     3061 tggatgttgg cgatcaattt acccttcgtt gcggcaatgc agcgcttgta ttacacaagg
     3121 atggctcgat tgagttttgc ggaaaacaac tgttactgca caccagcgat gttatgcaac
     3181 tcattggcag tggcattgat atgaatccgg atggcggcac agccatgact gcggatgata
     3241 ttgccccgtt acctacccac gacgtttctg aataaaaaat ataaaaggaa tttttctatg
     3301 accagccttt accgtattca ggagggctgc tttgccctgc ctgaaacttt tcttgatcgc
     3361 accgtcaata tcttcgtccc atcgggcaat gaaagggcaa ctcccagtct gaatattttc
     3421 cgcgatacgc tcaggccaga tgaaaatctg accacctata ttgaccgcca gatagccttg
     3481 atgaaaaaaa aaacctaggc cagcaccggg tactttcgcg gggaacggca ctggcgggaa
     3541 ctggcgaagc tgccatgaca ggtgaacaga ttctcgcaac tcatcgttct ggaaaaacag
     3601 aagtgtacca gcgtcaggcc gggtttatca ccgggccagc aaaggtacta atgttgacgc
     3661 tgaccactca gcgtcctttt gatgatcaca ccgaccagct atggactgca tggttgacta
     3721 gttttcagcc tgctaaaagc taaaaaaaac agggaggttt tgtcatgtat gaagccgcac
     3781 gggtggatga ccccatctac cacaccagcg cccttgccgg gtttttgata ggggccatca
     3841 taggtatcgc cattattgcc gtcgccagct ttgctttttt tacctgcggc tttttggccg
     3901 ggctgatttt gagttttctg gctgatcaga tagcttccgg agtcctgcaa ctgggtgagg
     3961 ctattgggcg ctcgatacat tccacagtgg gaaaaattct gaccggttct gataatgtca
     4021 gcaccaacag tcgcccggcc gcccgggccg tactgagtac ggtgatatgc gaagaccatt
     4081 ccccggaaat ccgcattgcc caaggttccg gcaacatcta catcaacagc cagctgcccg
     4141 taaggatgac cacactgagt gtgatgcagt gattgaggac ggctccccca atgtctttct
     4201 cggcggcggt acgcagacgg tactggaaat cagcccggaa atccctgact ggctgcgtca
     4261 ggtggtggat gtgctgtatg tggtcgccgg aatgcttggc gggctggctg gcgcctggcg
     4321 tcaggcggca aagatggggt cgaaatttgg tacaaaatgt gcggcaaaat ttattggtgg
     4381 cgaaatggtc gggatggcca tctctgacac agtcatggga ttgttcagca atccggtaga
     4441 tgtcaccact gggcaaaaaa ttttgctgcc ggagacggat tttaccctgc caggacgcct
     4501 gcctgtcacc tgttcgcgct tttacgccag ccacctggaa actgaaggat tgctgggcag
     4561 aggatggcgg ctgaactggg agataaacct gcgtgaggat gaaacatata tcaccttcat
     4621 cggtgtacag ggtcgggaac tgagttatcc gaaagagatg ctgataccgg gccaccagat
     4681 attcgacccg gaagagcagt tttacctgag ccgtctgcat gacggatgct atgtgctgca
     4741 ttacaccgac tgcagctatt acgtgtttga tgaatttgat gaccacggcg tcgcgccgtt
     4801 actttttatg gagacgccgt accggcagcg tattgctttc gggcgtgaga acggcaggct
     4861 ggtgcgggtg gcctccagca gcggacacca tctgttattg caccgcacga tgacgcaagc
     4921 aggggaacgc ctgtcgcata ttgaactttt gaagggcggg cgtcccggta acctggtgga
     4981 atatcgttac gacgataacg ggcaactgac tggcgtggtg aaccgggctg gtgtgacggt
     5041 gcgccagttt gcctatgaaa atggcctgat gacagagcat cgcaacgcga cgggctttac
     5101 ctgtacctac cactgggaag agattgaagg tttcccgcgc gtggtggaac acacgaccag
     5161 cgacggcgag gattaccggt tccactatga ttttgctggc gggcagacgg tggtaaccgg
     5221 caggccggag cagaagtggc agtggtggtt tgatgaagag acgtatgtga ctgcgcaccg
     5281 gacgccgggc ggcggaatgt accgcttcac gtacaacgaa aaccattttc cggtagcggt
     5341 ggaactcccc ggcgaacgca gggtaacgct ggaatacgac accctgagcc gggtggtgaa
     5401 agagacggac ccggcaggcc gggtgacaca gacgcagtgg aacggcagtt ttgctgaaat
     5461 cacgcgccgg gcgctggatg acgaccacgt atggaaggcg gattacaacg aacacggcca
     5521 ggtgatacgg gagacggacc cggaagggcg cgtcacgcgg tacgggtatg atgatcaggg
     5581 gctgccggag acggtctgcc accccggtaa acagcaggac cgttacacct ggaacgcgct
     5641 ggggttactg agcagccacc gtcgcatcac gggcagtgtg cagtcctggc agtatacgca
     5701 gcgcgggatg ctggcgcgcc atacggatga agagaaacgg gagacgcgct ggcagtacac
     5761 cccggaaggg ctggttgcca gtctgagcaa cggcaacggg gctcagtacc ggttcagcta
     5821 cgacggcgac ggacggctga cgggagaaca gcgcccggac ggcctgataa ggatgtttgc
     5881 gctgaatgcg gacgggtttc cggtcatcat cccgacgcag ggcacagagg gcggggtgcg
     5941 caatgaacag caggagcgtg atgcgctggg ccgcctgctg cgcagtgaca cgcagcacag
     6001 tacacggacc ttcagctaca accgtcttga ccagataacg gaagtgacgc tcacccccac
     6061 ggaagaaggg gaacggctgc accacatgca ggcggacacg gtgcgttttg cgtatgaccg
     6121 cagcgggtgg ctgacggcag agcacagcgt gcacggcagt atcaaatacc ggcgcgatgc
     6181 gctgggcaat cccaccgata tcacgttgcc ggacgggcag catctcagtc atctttacta
     6241 cggcagcggg catctgctcc agacggcact ggacggtatc acggtcagtg aatatgagcg
     6301 tgacagcctg caccgtcagg tgatacgcac gcaggggaaa ctggcgacgt tcagcggcta
     6361 caacgccgac aaccggttaa gctggcagcg cagcctgcca ggtggcagcc agaatccaca
     6421 gcaggcggta ctcccccggc gcagaacgac agcgtgacgg cgcgggacta tatctggaac
     6481 gcagacggtg aagtcggcgg catcaatgac aaactgcgcg gttgcctggt gttcagctat
     6541 gaccgcagcg gctggctgac cagccgcacg gggcagatgt atgaccatga ccattattat
     6601 tacgacaaag cgggcaacct gctgacggat gagtatcagg gagcggtgat ggataaccgc
     6661 ctgccgggtt acgggcgtga ccgctaccgc tataacgaat ggggcgaact gacagagcgc
     6721 agggaccagc agctggagtg gaacgcgcag ggccagttaa cgcgggtcat cagcagcaac
     6781 agcgagacgc ggtatcagta cgatgcgctg ggcaggcgca tcagcaaggc gaccagcaat
     6841 ctccataccg accggggaga acgaagccgg acaacgtttg tgtgggaagg gttccggctg
     6901 ttacaggaga caacgtggca gggaaagcgg acatacctgt acgatgcgga acagccgtac
     6961 acgccggtgg ctgccatcac ggggcgcgga gagagccaga aaatctggta ttaccacacg
     7021 gacctgacgg gcacggtaca cgaggtaacc gcgccggacg ggacgctggt gtgggcgggc
     7081 taccaggcgg gatttggtga aaaccggggc gacatcagca acagcggggc gtactttgag
     7141 cagccgcagc ggctgccggg gcagtacttt gacgaagaaa cagggctgca ttacaatctg
     7201 ttcaggtatt atgcgccgga gtgcgggagg ttcgtaagcc aggacccgat aggattaaac
     7261 ggtgggttga acttatatgc ttatgcgcct aatccgttag gttggatcga tccgctagga
     7321 ctgaattctt taggaataga ttcaaataat atatttagag gagatggtaa ctataaaggt
     7381 ggcggaattg gacagccaaa gagtggcaca atatcagcac aggacattat tgatcatctc
     7441 taagaaaaag gtgatgggcg tttaacatct tttagtacta aaaaaacatc tagttccaac
     7501 tctcgtggtg cagatttttt ctcaaaaaaa catgtcttca aagtggcagt ttctgatctc
     7561 gatgatttag ttaaaaataa tcagatcact gttttaacac aagaaaaagt aaaagcaata
     7621 tttggtttag aatcaagcaa gaaaattaga aaagaacttg gtaatattct tcagaatatg
     7681 gctaggaata atgaggtgtt agttcatggg caaattcttg gaaataaagt tagccgatgt
     7741 aattagttag tggagaggaa gttgtggacg aaattagaat aagaattgct ggtaatacgg
     7801 ctgcgccttg tttccatgtg ctaaaaaata agggatataa agtaacaatg gaaactcatg
     7861 cattatctga cgattgttta gattgtgtat atgattggta tgcggaaaaa aatggacaat
     7921 tttttagtgc aacgagtcca gaagagttac ttggattagt tgcaatgtgg gaagttcgtg
     7981 gtaatgactg gagagctaat ttgaatgaga taaatactta tcaaaaagcc atagaagaat
     8041 ccccgttttt tgataaaaac ggcaatgaaa tagagtctga ctaaatttta ttgatatagc
     8101 aacgataata accggaaatt tcttccagat ctttccggct ttttattatt taactttcgt
     8161 tcagcacact aaacatcccg tctcgcatcc ccttaactgg ctgtttaacc tgataaagtt
     8221 ccctgcgcag ttcctgtact tcgttgttat cccaagcagg atcaaacatc cctccgagat
     8281 cttcacggta acgcccgtgc cgatctcaaa tcccgctttt ctcagccatt tggcgctgat
     8341 tttgatcacc ggcgtgtcgc tgcggttggg cagataaccc acggtacagc gacgctgtgt
     8401 tttggaaatg gtgacttctg gcgtaatatt gtgtttaagt taggggcttt cgtaatctag
     8461 cccagccccc ggccctaaac acaaccccca ctcaccacaa cctaaactca tccgcatccc
     8521 gccatgccgg aaacttttct ctatattccc gcaatgccac catcgacagc tccgcatcaa
     8581 tacgcgttgc cttatgcgcg tcggcggtag cgataatctc gccttgcgga ttaatcaccc
     8641 ggctgtcacc gcgataatgg cagccgttgc catcgctgcc gacgcgattg catcccgcca
     8701 catacgcctg attctcaatc gcgcgggccg tcagcaatgc ctgccagtgc agagagcgcg
     8761 gagctggcca gttggcgacg tacagggcga ggtcataatc gttgagattg cgcgaccaca
     8821 ctggaaaacg taagtcgtag cacaccagcg gcaaaatacg ccagccgcgc cattccacaa
     8881 tcactcgcgc attgcccgct ttataatgca gatgctcatc tgccatgcgg aacagatgac
     8941 gcttatcata aaaatgtacc gtgccgcccg gctcaaccag caaaaagcgg ttaaccgaac
     9001 cagactccgt ttgtaatgca acactgcctg caatcagcgc attgcactgc tgcgccttag
     9061 ctgtcatcca gttcactacg tcatcttgtg caagcgacga agctgccgct tccatggcaa
     9121 agccgctggt aaacatctcc ggtagaacga tcacatcgcg cccggtaata ccttccagtt
     9181 gacgatcaaa atggcgcagg ttggcaggac catccaccca caccaacggt tgctgcaaaa
     9241 gcgtaatctt caaactaggc acggtgtaca actcctttat gcgaagggtt ttataacttt
     9301 aacaccttat caggcagttg ccttagcgca gaataaattg ataacaaatg ctgatattgg
     9361 aaatatctga tttgcaaatt atcgtgttat cgctaggctt taggaggtta ataacatggg
     9421 caggataagc tcgggaggaa tgatgtttaa ggcaataacg acagtcgccg cactggtcat
     9481 cgccaccagt gcaatggcgc aggatgattt aaccattagc agccttgcaa agggcgaaac
     9541 caccaaagct gcgtttaatc agatggtgca agggcataag ctacctgcat gggtgatgaa
     9601 aggcggtact tatactcccg cacaaaccgt aacgttggga gatgagacgt atcaggtgat
     9661 gagcgcgtgc aaaccgcatg actgtggctc gcaacgtatc gctgtgatgt ggtccgagaa
     9721 atctaatcag atgacggggc tgttctcggc tattgatgag aaaacgtcgc aagagaaact
     9781 cacctggctg aatgtgaacg atgcgctttc gattgatggt aaaacggtgc tgttcgcggc
     9841 gttgaccggc agcctggaaa accatccgga tggctttaat tttaaataat tagcggataa
     9901 agaaacggag cctttcggct ccgttattca attacgcggc ttcaactttc cgcactttct
     9961 ccggcaactt taccggcttc gtcgccagct cttccggatc aaagtcatca acgttaatac
    10021 tgcgcagacg gctttcttcc gctttcacca gaatagcggc ttcatcttta tcaatcagcc
    10081 ccttcgtcag cgcgttgtgc gccagttcat ccagacgggt aaatggcaga tttttaccca
    10141 gctctttaca gatccgctga tgaatcgggt cggcggcaat cacatccacc aacgcctctt
    10201 ccagtaaacc aaccgggtta tgctcgcttg gcgtcaggta ctgaccgcga ccaatacgtg
    10261 aacgagtggc gttcggcact tgtaaaatct tcgccatttt atgatccagc ttgtcagacg
    10321 gtgccagata atgacgtccg gtcgggaaga tcaccacatt cagcagcccg gcaaccacgc
    10381 ggttcgggaa gttttgcagt aaattgtcca ttgcctgttc tgcctgatac agcgcatcct
    10441 gtacgcccca gtgcaccagc ggcagatcgg cttcattacg gccttcgtcg tcataacgct
    10501 tcagaacggc agaggcgagg taaagctggc ttaaaatatc ccccagacgt gccgagatac
    10561 gctcacgacg tttcaggctg ccgcccagca gtgccatcga gacatcagaa agcagggcga
    10621 ggttggcgct caggcggttc aggtgctgat agtagcgttt agtggcatcg ccggttggcg
    10681 tgctgctggt taaaccgcgc gtcaggccca gccagaagct gcgaactttg ttgctaccga
    10741 cgtgaccgat atgtttgaac aacagtttat cgaacgcgtt gacgtcattg ttcttcgccg
    10801 cttccatctc ttccagcacg tacggatggc aacgaatcgc tccttgtccg aagatcatca
    10861 tgctgcgggt cagaatgtta gccccttcaa cggtgatggc aatcggtgcg ccctggtaag
    10921 cacgcgccag gaagttgctt tgcccgagca taatgccttt accgccggta atatccatcg
    10981 catcaataat cgactgctgc ccgcggtggg tacagtgata cttaacgata gccgacagca
    11041 cggcaggttt ttcgccgagc ataatgccgt aggtaatcag cgatgccgca gcatccatta
    11101 cataagcgtt cccggcaata cgcgccagcg gctcttcaat cccttccatc ttaccgatag
    11161 agattttgaa ctgacggcga atgtgagaat acgcgcccgt tgccagcgct accgatttca
    11221 cgccgccggt tgagttggaa ggcagggtga tgccgcggcc taccgagagg cactccacca
    11281 gcatccgcca gccttgcccg gccattttcg gcccgccgat gatgtaatcg atcggcacga
    11341 agacatcttt accgcgcgtc ggtccgttct ggaacggtac gttcagcggg aagtggcgac
    11401 gaccaatttc cacgcccggc gtggtggttg ggatcagcgc acaggtaatg cctaaatctt
    11461 ctgcaccgcc gagtaatttt tccgggtcgg agagtttaaa cgccagccca agcacggtcg
    11521 caatcggtgc cagcgtaatg tagcgtttgt tccaggtcag acgcatcccc agcacctgct
    11581 ggccctgcca ttcgcccatg cagacaatcc cggtgtccgg aatcgcgccc gcatcggaac
    11641 ccgcttccgg gctggtcagt gcaaagcagg ggatctcctg accacgcgcc agacgcggca
    11701 gatagtgatc tttctgctcg tcagtgccgt aatgttgcaa cagttcgccc gggcctaatg
    11761 agtttggcac gccgacggta atcgccagga tcccgctcac gccagagagt ttttgcagca
    11821 cacgagactg ggcataagct gagaactcca gtccgccgta ctcttttttg ataatcatcg
    11881 cgaagaagcg atgctcttta aggtacgccc acaactccgg tggcagatcc gccagctcat
    11941 gagtaatctg gaaatcattc gccatccggc aggcttcttc taccgggccg tcgagaaacg
    12001 cttgctcttc agcggtcagg cgcggctgcg gatagttatg cagctttttc cagtccggct
    12061 tgccctggaa caagtcgccc tcccaccagg tggtgcccgc atcaatcgct tctttctcag
    12121 tgcgcgacat cggcggcatc accttacgga aaccgcgaaa taccggcgcg gaaatcatcg
    12181 acttacgcat aggcgcaaag ttaaatggca cgaggataat ggtcagaggc accagtaccc
    12241 acgccgacca cagaccagca acgccgaggg cggctgtcca ggcgagcaaa atcagactgc
    12301 tgataaataa gctcacgcgg tgatagaaca acgcgccgag caggacaacc gtagcgagaa
    12361 tactcaaaat catcataacg aaaagcccct tacttgtagg aggtctgacc acttgtgatg
    12421 atatggttgt agtggatgta aaaacattta gcaatgtgtt tacaatataa ttacaacaaa
    12481 gctcacattg ttgctgtttt tatccgcact tcaggtcaaa aagtcctggt catagcacct
    12541 gcccgtactt ctcgcttttg gcggtatccg gtacactgca ttttgtctat tacatttatg
    12601 ctgaaggata tcctcatgta ccaggatctt attcgtaacg aactgaacga agcggcggaa
    12661 acgctggcta actttttaaa agatgacgcc aatattcacg ccattcagcg cgcggcggtc
    12721 ctgttagcag acagctttaa agccggtggc aaagtgcttt cctgcggcaa cggcggttcc
    12781 cattgcgacg ctatgcactt tgccgaagag ttgaccggtc gctaccgtga aaaccgtccg
    12841 ggctacccgg cgattgctat ttctgacgtt agtcatattt cctgcgtcgg taatgatttc
    12901 ggtttcaatg atattttctc ccgctacgtt gaagcggtag gtcgcgaagg cgatgtactg
    12961 ctggggatct ccacctccgg taactctgca aacgtgatca aagcgatcgc agcggcgcgt
    13021 gagaagggga tgaaagtgat caccctgacc ggtaaagacg gcggtaaaat ggctggcacg
    13081 gcggatatcg aaattcgcgt tccgcacttt ggttatgccg accgcattca ggagattcac
    13141 attaaagtga tccatatcct gatccagtta attgaaaaag agatggttaa gtaagtctgg
    13201 cgtaggccgg ataaggcgtt tacgccgcat ccggcacttg tgcgctgatg cctgatgcga
    13261 tgctgacgcg tcttatcatg cctacaaatc tgtacccgaa ccgtaggccg aataattcgt
    13321 ttacgccgca tccgacctga aaattcttaa atcaatcttc gccggggtcc atgcgctccc
    13381 gctgttgtgg aggttaccca tgtgcgaatt gctcgggatg agcgccaacg tccctaccga
    13441 tatctgcttt agtttcaccg gacttgtaca gcgtggtggt ggaaccgggc cacataaaga
    13501 tggctggggc attacctttt acgaaggtaa aggctgtcgc acatttaaag atccacaacc
    13561 cagctttaat tcccccatcg ccaaacttgt ccaggactac ccgataaaat cctgttcggt
    13621 ggtggctcat attcgccagg ctaatcgggg cgaggtggcg ctggaaaata ctcacccatt
    13681 tacccgcgag ttatgggggc gtaactggac ttatgcccat aacggacaac tgacgggcta
    13741 caaatcactg gaaaccggca acttccgccc ggtcggtgaa accgacagcg aaaaagcctt
    13801 ttgctggctg ctgcataaat taacgcagcg ttacccgcgc acgccgggca acatggcggc
    13861 agtgtttaaa tatatcgcct cactggcgga tgaactgcgg gcgaagggcg ttttcaacat
    13921 gctgctttcg gacgggcgct atgtaatggc gtattgctcg actaatttac actggatcac
    13981 ccgccgcgcg ccgtttggcg tggcaacgtt gctggatcag gatgtggaaa tcgacttcag
    14041 ctcgcagacc acaccgaatg atgtggtcac ggtgattgcg acacagccgc tgacgggcaa
    14101 tgaaacctgg caaaaaatta tgccaggcga atggcgctta ttttgcctcg gggagcgtgt
    14161 agtttgatgc cagttgtggc tgcaccactt cgtggcttaa cggcttgctg accacgtaac
    14221 ggccattggc cacagaaacg gtcggtggct tacgggtttg ctcaaagtag tcgtagcccg
    14281 gcttcagctg ctcccagaag tccttaaagt tggaatattt atggcgcttc atattggcgt
    14341 cggtcatgcg gaatgggtaa atactcacct gcacgctcgg ctgaccaaac accagcgcac
    14401 cagtaacgaa ctggaagatc tcatcaatac cctgattggt cattgcgtag cagccgatgg
    14461 atacacaatc gccgtggatc atcaggtatt tcccttcata accatgcgca cggtcataag
    14521 cattggggaa tccaatatta atcgctttgt agtaacggct gtctggtttt aactgattac
    14581 gctggacgct ataaaaccct tccgggcttt tgaaatcgcc ctgacgctgt tttggcccta
    14641 agccgccgga atatttacag attttataac tgtcgagcag ttgatattgc tcgcccattt
    14701 tgacgtaaag atcgagcgta cgttcttcct tgaagatctg gatgtagaca ggggatccca
    14761 tcaactgctg cttatactct ttgctcactg gcgtggtcga gctactgctg cccagcaaac
    14821 cggcaaacga aacgcacggg atcaacaaca tcgcaagaat taatgcgatt ttacgcatac
    14881 tgcttattcc ttgataaaac ggttacacac gccaggacgg caaaatggat cccaaatcgg
    14941 aatagtctgg atttggaagg ctcacattat caccaaaaga gttttacgca agcctgtcgg
    15001 cgctgggttt acaaatttag cacaggggca gttttcaata gtccgaaaag cgcatgtatc
    15061 atttatttcc taatattatg aaatctcaat agtaatatgc aaataagcaa ttaataatcc
    15121 cgtttactcc tccggtcttt taaaattctc accttcattc agccggttta tgtctttcat
    15181 gtcttcgttc ttgctcggca gatttttgca tccaggcgtg ttttcgttat gcgttttgct
    15241 ccctttactc gccagcgcca ccacaaccgg gagcagataa aacagcatcc agaatggttt
    15301 cctgcgccgc tcaaggcgag tgacagacgc tggcaggcgc tggcggaaaa caaccacttt
    15361 ttaagcagcg atcacctgca taacatcacc gaagtggcga ttcaccgcct ggagcagcag
    15421 cttggcaagc cttacgtctg gggcggcacg cggcccgatc aaggcttcga ttgtagtggg
    15481 ttagtttttt atgcctacaa caagatcctt gaggcgaaac tcccgcgcac ggccaatgag
    15541 atgtaccact accgtcgggc aacgattgtg gcgaataacg acctgcgccg gggagatttg
    15601 ctatttttcc atatccacag ccgcgagatt gccgaccata tgggggtgta tctgggcgac
    15661 ggtcagttta ttgagtcact gcgcaccggg gaaaccattc gggtaagccg gttagccgaa
    15721 cctttctggc aggaccattt tttaggcgcg cgcaggattt tgacggaaga gatgattttg
    15781 taggccggat aaggcgttta cgccgcatcc ggcggttgtg cacggatgcc tgatgcgacg
    15841 ctgacgcgtc ttatcatgcc tacaaatctg tgcgtttttg cgagccgctt tcccgatata
    15901 acacagcgtc attgtcggcc tttccttttc ctcttaccgt tgagaaaaag gagtcgctat
    15961 gtcggaatat cgtcgttatt acatcaaggg gggaacatgg tttttcacgg taaatttacg
    16021 caatcgtcga agccatcttc tgaccaccca gttccagacg ctccgtaacg ccattattaa
    16081 cgttaagcga gacagacctt ttgaaattaa cgcctgggtc gttttgccgg agcatatgca
    16141 ttgcatatgg acattacccg aaagcgatga tgatttttcc tctcgctggc gggagattaa
    16201 aaagcagttc acccatgctt gcggattgaa aaatatctgg caaccgcgtt tttgggagca
    16261 tgccattcgc aatacaaaag attatcgcca tcatgttgat tatatttata taaatccaga
    16321 gaaacatggc tgggtaaagc aggtgagtga ttggccattc tcaacgttcc atcgcgatgt
    16381 cgcgcgaggg ttatatccca tcgattgggc gggagacata acggatttaa gtgccgggga
    16441 gcgtatcatt ttataactgt gcgctggtgc cggatgcgct tacgcttatc cggcctacaa
    16501 atcgtgcttt aacggtaggc cggataaggc gttcacgccg catccggcag ttatgcgcag
    16561 atgcctgatg cgacgctggc gcgtcttatc atgcctaaaa acttgtgcct gaacggtagg
    16621 ccggataagg cgtttacgcc gcatccggca attgtgcacc gatgccggat tcttacatca
    16681 acgcccccat aatcttcaac tccagctcat ccggcacttc gttatacgac agcacatgca
    16741 gccccggcgc aaacaacctt gcataacgcg gcagcaaagg gcgtagctgc ggcggcacca
    16801 gcagcaccgg atctttcccc gccgctttca tctgctcttt cacctgtggc atcgtgctct
    16861 ggaactggtt gagcatattc gggtcaaccg gcacgctgtc gagcatcact ttgccggcct
    16921 gttgcgcctg attcactacg ttggtcagca gattttccag ctcattattc agcgtataca
    16981 ccgtcagctc ctgcttgcga acgaacggat gagtaatgct gcgccgcagc gccaggcgca
    17041 catcggccgc cagcagaata tgatctttag tcaccgtgct actggcgacc agcacggtgg
    17101 cgatggtgac gatatcgcgc agggaaacgc cttcggtcag cagcgcccgg tacactttca
    17161 gcaattggct gtaattgagc gccgcgctta aatcctccgc caggcgcggt gccatcgacg
    17221 acaaacggtt atgcaactgc gtgatgtcat catagttaaa caaatcagga atatagctgc
    17281 gcacaatctt gttcacatgg gtggcaatca cactggcgct gtcgatcacc tgatacccca
    17341 tattcagagc cttcgctttc tgcgcaggct ggatccaggt gaccggtatc ccgtacgccg
    17401 ggtcgttccc cagcacgccg tcaatctcgc cgtaggtttc gctggagggc agcgccatca
    17461 gtttatccgc cggaatatcc gcttcatcag cctcaatgcc gttgatgaaa atagcgtact
    17521 ggctgggctt gaggcggaag ttttcccgaa tgcggatctc cggcagcagc acgccgttac
    17581 cgtcggaaat cacctgccga acgccgcgaa tccgctgggt gagcgggttg ccctgcgctt
    17641 tgtccaccag cgccaccagc ttgtagccga ggcttaagct gatgggctcg atcagcggaa
    17701 tagtttccca actgacctgt tgttcgctgg tttcagtgat ggtgcgggtc agcgtttcga
    17761 ggcttttctc ttccgcctcc gccgcctgcg ggcgtttgct catccgccag ccggtaaagc
    17821 caagcagggt gctgaacagc aaaaacggca gatgcggcat ccccggcacc accgccagca
    17881 cgaacatgat cccggtggcg gtgtagagca ccgacgggct tgccagcaac tggtggcgca
    17941 cgtcatgggc gatatcgccg ttgtcgctga cgcgggtgac gataatcgcc gccgcggtgg
    18001 agagcagcag ggaagggatc tgcgccacca ggccgtcgcc gatggtcatc agcacgtatt
    18061 gctggaaggc ggcgtcggcg ctcaggttgt atttgaagat gccgatacag acgccgccgc
    18121 tcaggttgat cgccagaatc atcatcccgg cgatggcgtc tccgcgtaca aacttcgatg
    18181 ccccgtccat cgcgccgtag aaatcggcct cgctggcaac atctttacgc cgggtttgcg
    18241 cctgcgcctg gttgatcaat ccggcgttaa gatcggcgtc aatcgccatc tgtttgccgg
    18301 gcatcgcgtc cagggtaaag cgggcagaaa cctcggaaat acgctcggcc tctttggtga
    18361 cgacgataaa gttgattgtc aacagcgtca gtaaatccga gcgtgaaagc attatcgccg
    18421 cgctgcacgg gcagtcgatt tttaacggtg gtgggctgtc gccgttaaat aaaatcagcc
    18481 cgtctcatcc gccaaaacct gccaccgtgg cggtgccgga agagacggag aaaaaagcgc
    18541 gtgatgtgaa tgagaaaacg gcgctgctga agaagaaatc cgccaccgag cttggcgagt
    18601 tggcaaccag tatcaacacc attgcccgtg atgcgcatat ggaagcgaat ctggagatgg
    18661 agattgttcc ccagggatta cgcgtgctga ttaaagacga ccagaatcgc aatatgttcg
    18721 aacgcggcag cgcgcagatt atgccgttct ttaaaacttt gctggtggag ctcgcgccag
    18781 tgttcgactc gctctataac aaaattatta ttaccgggca taccgatgcg atggcctaca
    18841 aaaacaatat ctacaacaac tggaaccttt cgggcgatcg cgcgctttca gctcgtcggg
    18901 tgctggaaga ggccggaatg ccggaagata aagtgatgca ggtaagcgca atggcggacc
    18961 agatgctgct ggacgcgaag aatccgcaaa gcgcgggcaa tcggcgtatt gagattatgg
    19021 tgctgaccaa aagtgcgtcc gatacgctgt atcaatactt tggtcagcat ggggataaag
    19081 tggtgcagcc gctggtgcaa aagctggata agcagcaggt gctttcgcag cgaatgcgct
    19141 aaatgctgaa tctttacgca tttctcaaaa cctgaaatca ctgtatactt taccagtgtt
    19201 gagaggtgag caatgcgtaa aatcattcat gtggatatgg actgcttttt cgcagcggtg
    19261 gagatgcgcg acaatcccgc tctgcgcgat atccctattg ctattggcgg cagccgcgaa
    19321 cgtcgggggg tgatcagcac cgccaattat cccgcgcgta aatttggcgt acgtagcgct
    19381 atgcctactg ggatggcgct caaattatgc ccacatctca ccttgcttcc ggggcgcttt
    19441 gacgcctaca aagaagcctc aaatcatatc cgtgaaatct tctcgcgcta cacctcgcgc
    19501 attgaaccgt tgtcactgga tgaggcttat ctcgatgtca ccgatagcgt ccattgccac
    19561 ggttctgcga ccctcatcgc ccaggaaatc cgccagacga tcttcaacga gctgcaactg
    19621 acggcatctg cgggcgtggc accagtaaag tttctcgcca aaatcgcctc cgacatgaat
    19681 aaacccaacg gtcagtttgt gattacgccg gcagaagttc cggcattttt acaaacctta
    19741 ccactggaaa aaatccccgg cgtcggcaaa gtctcggcgg caaaactgga agcgatgggg
    19801 ctgcggacct gcggtgatgt acaaaagtgt gatctggtga ttctgcttaa acgctttggc
    19861 aaatttggcc gcattttgtg ggagcgtagt caggggattg acgagcgcga cgttaacagc
    19921 gaacggttac gaaaatccgt cggcgtggaa cgcacgatgg cggaagatat tcatcactgg
    19981 tctgaatgtg aagcgattat cgagcggctg tatccggaac ttgaacgccg tctggcaaag
    20041 gtaaaacctg atttactgat tgctcgccag ggggtgaaat taaagttcga cgattttcag
    20101 cagaccaccc aggagcacgt ctggccgcgg ctgaataaag ctgacttaat cgccaccgcg
    20161 cgtaaaacct gggatgaacg ccgcggcggg cgcggtgtgc gtctggtggg gctgcatgtg
    20221 acgttgcttg acccgcaaat ggaaagacaa ctggtgctgg gattatgatg tatactatta
    20281 tgtatattct ggtgtgcatt attatgaggg tatcactgta tgcatcgaat tctcgctgaa
    20341 aaatcggtca atatcactga gttacgtaaa aacccagcta aatactttat tgatcaacca
    20401 gttgcggttc tttctaataa tcgccccgca ggatatctct taagtgccag cgcattcgaa
    20461 gcgttaatgg acatgcttgc tgaacaagag gagaaaaagc ccataaaggc gcgctttcgt
    20521 ccaagtgctg caagattaga ggaaattaca cgccgcgcta aacaatatct taatgatatg
    20581 acggatgatg atttcaatga ctttaaggaa taaggatgcg ggtattcaaa acaaaactta
    20641 ttcgcctgca acttacagca gaggaacttg atgcgttaac ggcggatttt atttcctata
    20701 agcgtgacgg tgttttgcca gatatatttg gtcgcgatgc actctacgac gactcgttta
    20761 cctggccatt aatcaaattt gagcgagttg ctcatattca tctggcaaat gtgaataatc
    20821 catttccgcc acagttgcgc caattcagca gaacgaatga cgaagcgcat ttggtatatt
    20881 gtcagggtgc gtttgatgag caagcatggt tgctcattgc cattctgaag cctgaacctc
    20941 ataaactggc tcgagataac aaccaaatgc ataaaattgg aaaaatggca gaagcgtttc
    21001 gcatgcgttt ttgaatttat attatgaata acatacaaat aagagattat cagcctggcg
    21061 attttcagca actatgcgct attttcatta gagcggttac gatgaccgtc agtcagcatt
    21121 attcactaca acaaattgcc gcctgggcgc agattgacga atctcgctgg aaggagaaac
    21181 tcgcgaaatc acaagtgcgg gttgcagtta ttaatgcaaa actggtcggc tttattacct
    21241 gcgttgaaca ctatatcgat atgttatttg ttgaccctga atacacccgc cgtggggttg
    21301 ccagcgcttt gttaaaacct tttattaagt ctgaatccga acttacggtg gacgcaagca
    21361 taaccgcaaa accctttttt gaacgttatg gttttcagac agttaagcag cagcgcgttg
    21421 aatgccgggg agcgtggttt attaattttt atatgcgtta taaaccgcaa cattaaatcc
    21481 agcttgcaat gaaaataacg cccgcctagt atgtgcgcgt tttcttattc cacaaactgc
    21541 aaggaggtaa atcatgacaa atcctttatc aatgactctt tgcagacctt tccaggatta
    21601 attctttttt tcttgccctg gattcgtctg ccatttcctg attttttaat tgttactctc
    21661 aataattcaa gtcgcagcac atgcaacttg aagtatgacg agtctattta cggaatggta
    21721 tatgggcaat tatattcgtc ccttatccga tgcggtattt accatcgcat ctgatgacct
    21781 gtggatcgag agtttagcga tccaacaatt acacaccacg gcaaatttac ccaacatgca
    21841 gcgcgtagtg gggatgccag atttacaccc cggacgcggc tacccgattg gcgcagcgtt
    21901 cttctccgca ggccgttttt acccagcact ggtcggcaat gatatcggct gtggcatggc
    21961 gctatggcaa acagatattc tcgctcgcaa atacaacgcc gataagtttg aaaagcgatt
    22021 atcagctttg gatgacgttg ctgaagaaag ctggctggag gaaaacctgc cgtcagcatt
    22081 tgcacagcat ccgtggcgca gctcgcttgg ctctatcggt ggtggcaatc atttcgctga
    22141 actgcaacaa gttgatcaaa tcatcaacgc tgaactgttt gcactggcag gtctggatgc
    22201 gcagcatctg caactgctgg ttcatagcgg ctcgaggggg ctagggcagt ctattttaca
    22261 gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg acgcgctggc
    22321 gtttgcgcgt tttaatcgcc acctgatcgc tttgcgcata atgcaacagg ttaaggccac
    22381 cggaagtccg gttctggatg tggcgcataa ctttgttagc gcgtgtcgaa tcggtgatca
    22441 acaaggggtc ttacatcgta aaggtgccac gcctgatgac tgtggtctgg tggttattcc
    22501 cggttcacgc ggtgattact ccaggctggt tcagcccgtc aggagtgagg aaacattgca
    22561 ttcgctggcg catggggctg ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc
    22621 agcgaaatac acagcgacgc aactctcgcg tactgaactt ggcagtcggg taatttgtcg
    22681 cgataaacaa ctcatcttcg aagaagcgcc acaagcctat aaatcggctg aaagtgtggt
    22741 gcaatgtcag ggcaggccgg attaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg
    22801 ctcaaaaaca gtggagggaa aaaaggatga tcttgctata actctcctca gcgcaggggc
    22861 cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc
    22921 gacaagacgt cgcggtaacg gtgctggaaa cagaaacagg ccgctactct gacacgctgc
    22981 gttcggcgct gatttctctg gatggcgaca acgcatgggc gttaagcgaa agctggtgtg
    23041 gcactattca gtggatttgc ccgagcccgt atcgcccaca tcacgggcgt aaaaactggt
    23101 ttctgggcat tgggcgtttt accgctgatg agcaggagca atccgatgcg atccggtatg
    23161 agacgctgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac
    23221 gcgccacgca tttggcatcc ggtattagcg tgaaggttca gtcagagcgc agccagcatg
    23281 ctaacaaacg gctggcgcga ttgctgattg cctggaagct ggatcagcag caataggaaa
    23341 atagcgcggt gctgaaatca cagcggcgaa tgttccatca ccagattgaa cgtggcaacc
    23401 cgcgacgaac gtttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg
    23461 aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca
    23521 gcagttcagt cagcagtgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg
    23581 gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt
    23641 ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggc gtcttgttga
    23701 acaggcgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc
    23761 agccaggata tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc agcgaatcca
    23821 gcatgctcac cacgtagtct ttaccgctgt cgattagtga acggatcagg cagtgaattt
    23881 ctacgttatt gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt
    23941 ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg
    24001 tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg
    24061 ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca
    24121 gagatttcag tgcgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg
    24181 ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgcaggtcc agttcttctg
    24241 catgacccgc caggaagcgc accagcagtt tgttggcatt acccaggcca acgtggattt
    24301 caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag
    24361 ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac
    24421 ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc
    24481 agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca
    24541 cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagtgca gaggccatac
    24601 caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat
    24661 aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca
    24721 tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt
    24781 gacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc
    24841 aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga
    24901 ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa
    24961 gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt
    25021 gtcaacacaa gacaggcttg cgagatatgt attgagaata ccactttatc ccgcgtcagg
    25081 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
    25141 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
    25201 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
    25261 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
    25321 aagccgtggc ggtctggtac cgggggcgtt actggcgcgt gaactgggta ttcgtcatgt
    25381 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
    25441 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
    25501 tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
    25561 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
    25621 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
    25681 tcaacgcctg gcattgccgg gcgttgttct ttttaacttc aggcaggtta caatagtttc
    25741 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
    25801 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
    25861 aaccgcctgt gcagtcggct cttgatggta aaaccatccc tcactggtat cgcatgatta
    25921 accgtctgat gtggatctgg cgcggtattg acccacgtga aatcctcgac gtccaggcac
    25981 gtattgtgat gagcgatgct gaacgtaccg acgatgattt atacgatacc gtgattggct
    26041 accgtggtgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
    26101 cctgtacgga agaagatccg caactcagtg gtcgacactg gctgcatgcg gctacgttgt
    26161 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
    26221 tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg cggcagatgg
    26281 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
    26341 gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
    26401 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
    26461 cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg
    26521 tcttaaaggc gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
    26581 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatacct tgaatcgccg cgtctgaaag
    26641 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
    26701 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
    26761 acgaagcgtt acgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctggggc
    26821 gtcgctgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
    26881 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
    26941 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac
    27001 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgca tcacaacagg
    27061 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
    27121 cactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
    27181 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgaattctgg ggctggtgga
    27241 tggagctgga agcgcaggaa tcccgtttta cctacagcta ccagtttggt ctgttcgata
    27301 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
    27361 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagc
    27421 tggaaccggc gaatgatttt cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg
    27481 ccggatgcat cacatccggc aatattcatt aaaactgata cgtcatccca accgcgacag
    27541 tatcatcatt attaatattc agcttattat cgctatccag ttggttgatt ttataatcaa
    27601 caaacgctga catatttttg ttgaaataat acgtagcacc gacgtcgata taattgacca
    27661 gatcttcatc accgatgccc tcaatatctt tccctttcga taagacataa cccagcgatg
    27721 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
    27781 caaagccgcc agttattggc gtcattttgc gtgtttcaga atagaaagtt gccagataaa
    27841 tattattggc atcgtatttc agacctgttg cccatgcttc tgcacgcttg cctgtgccac
    27901 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
    27961 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgc
//
LOCUS       DAJTYH010000053        27816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTYH010000053 DAJTYH010000000
VERSION     DAJTYH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..645)
                     /locus_tag="OO609_002200"
     CDS             complement(<1..645)
                     /locus_tag="OO609_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6633605.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHCTRFGNQVYAIGGNATSANLMGISTRSTTIRIYM"
     gene            complement(632..1654)
                     /locus_tag="OO609_002201"
     CDS             complement(632..1654)
                     /locus_tag="OO609_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6633606.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(1668..3170)
                     /locus_tag="OO609_002202"
     CDS             complement(1668..3170)
                     /locus_tag="OO609_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6633607.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3310..4266)
                     /gene="ytfQ"
                     /locus_tag="OO609_002203"
                     /pseudo
     CDS             complement(3310..4266)
                     /gene="ytfQ"
                     /locus_tag="OO609_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4576..5106
                     /gene="ppa"
                     /locus_tag="OO609_002204"
     CDS             4576..5106
                     /gene="ppa"
                     /locus_tag="OO609_002204"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6633608.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5486..5827)
                     /locus_tag="OO609_002205"
     CDS             complement(5486..5827)
                     /locus_tag="OO609_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6633609.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(5830..9609)
                     /gene="tamB"
                     /locus_tag="OO609_002206"
     CDS             complement(5830..9609)
                     /gene="tamB"
                     /locus_tag="OO609_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6633610.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9606..11339)
                     /gene="tamA"
                     /locus_tag="OO609_002207"
     CDS             complement(9606..11339)
                     /gene="tamA"
                     /locus_tag="OO609_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6633611.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11545..12183
                     /gene="msrA"
                     /locus_tag="OO609_002208"
     CDS             11545..12183
                     /gene="msrA"
                     /locus_tag="OO609_002208"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6633612.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12506..13849
                     /locus_tag="OO609_002209"
     CDS             12506..13849
                     /locus_tag="OO609_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6633613.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13911..14117)
                     /locus_tag="OO609_002210"
     CDS             complement(13911..14117)
                     /locus_tag="OO609_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6633614.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14442..14996
                     /locus_tag="OO609_002211"
     CDS             14442..14996
                     /locus_tag="OO609_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6633615.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15059..15997)
                     /locus_tag="OO609_002212"
     CDS             complement(15059..15997)
                     /locus_tag="OO609_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633616.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16209..16949)
                     /gene="cysQ"
                     /locus_tag="OO609_002213"
     CDS             complement(16209..16949)
                     /gene="cysQ"
                     /locus_tag="OO609_002213"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6633617.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17139..19082
                     /gene="cpdB"
                     /locus_tag="OO609_002214"
     CDS             17139..19082
                     /gene="cpdB"
                     /locus_tag="OO609_002214"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6633618.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19208..19588)
                     /locus_tag="OO609_002215"
     CDS             complement(19208..19588)
                     /locus_tag="OO609_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6633619.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            19677..20537
                     /gene="qorB"
                     /locus_tag="OO609_002216"
     CDS             19677..20537
                     /gene="qorB"
                     /locus_tag="OO609_002216"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6633620.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            20646..21611
                     /locus_tag="OO609_002217"
     CDS             20646..21611
                     /locus_tag="OO609_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6633621.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            21719..22381
                     /gene="ytfE"
                     /locus_tag="OO609_002218"
     CDS             21719..22381
                     /gene="ytfE"
                     /locus_tag="OO609_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6633622.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22426..23838)
                     /gene="cycA"
                     /locus_tag="OO609_002219"
                     /gene_synonym="dagA"
     CDS             complement(22426..23838)
                     /gene="cycA"
                     /locus_tag="OO609_002219"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6633623.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24146..24766)
                     /gene="fklB"
                     /locus_tag="OO609_002220"
     CDS             complement(24146..24766)
                     /gene="fklB"
                     /locus_tag="OO609_002220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6633624.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            24985..25530
                     /locus_tag="OO609_002221"
     CDS             24985..25530
                     /locus_tag="OO609_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6633625.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25514..26161)
                     /locus_tag="OO609_002222"
     CDS             complement(25514..26161)
                     /locus_tag="OO609_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6633626.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26373..27164
                     /locus_tag="OO609_002223"
     CDS             26373..27164
                     /locus_tag="OO609_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6633627.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27174..>27816
                     /locus_tag="OO609_002224"
     CDS             27174..>27816
                     /locus_tag="OO609_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6633628.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDW"
BASE COUNT         6815 a         7398 c         6868 g         6735 t
ORIGIN      
        1 catataaatg cgaatagtgg tgctgcgagt agaaatcccc atcaggttcg ccgacgtcgc
       61 gttgccgcca atagcgtaca cctgattgcc aaaacgggta caatgcgcaa ggaaaatacc
      121 gataaccacc accgccagca tcaacagccc catcgcacta aggcgaccgc cgccggggat
      181 tttccacgca aggcttgaga gcgtgtcata aatgggatgg tttatcggaa tcgactcttc
      241 cgaaacgaga tagctgacgc cgcgcaaaaa gaacatcccc gcaagcgtaa tgataaatgc
      301 cgggatcttc agggcgtcga tcagaagccc cataaatgcg ccgaaggcac agcccatcac
      361 cagcactagc ggaaacgcca gcagcggcga gaggccgaaa tcgccaacca cttttgccag
      421 aaacacgcca gtaaaggcga tcaccgaacc gacggagaga tcgataccgc cagagaggat
      481 cacaaaggtc atgccaacgg cgattatccc aagaaaggcg ttatcggtca ggatattgca
      541 gatcaccctt gtggaagcaa aaccgggaaa ctgggtcagg cagtagagat agcccagcac
      601 aaagacgccg atggtgatca tcagcggcaa gttacgtttt atcatggcta cgtactcctt
      661 taatcagact gataaagcgt tgcgactgaa caatcagcac acaaagcacc accaccgctt
      721 tcaccacctg gttcatctcc ggcggaaagc ccgaaagcaa aattccggtg ttcatcccct
      781 gaataatcag cgcccccact accgagagca gcaggttaaa gcgcccgccc atcagcgatc
      841 cgccgccaat caccaccgcg agaatggcgt ccagctccag ccataatccg gcgttgttgg
      901 catcggcacc gcgaatatcc gccgccacga taatgcccgc aatcgccgca catagcccgc
      961 tcaacacgta ggtgagcatg acgatgattc gcgtgtttac cccggcattt tttgccgccc
     1021 gaatgttgat accaacgact tcgataaaca tccccagcgc cgttttgcgg gtcaacagcc
     1081 agaacaggag aagcgtcagc accgcgataa tgaccggcgt tggcaggaac aacagcgatc
     1141 cgctgccaaa ccatgagaga tccggcgagt taaacgtgac gatctgcccg gcggtgatca
     1201 gttgcgccac gccgcgcccg gcgaccatca ggatcagggt ggcgacaaac ggctgaattt
     1261 tgaggatcgc taccagtatg ccgttccaca atcccgccag gatgccggtg cccagggcgc
     1321 ttaacaaaac aatcggcagg ctgaatcccg cgaccgtcat cgcagccgtt gtggctccgg
     1381 cgatagccat caccgccccc acagagagat cgataccacc ggtggcgatc accagcgtaa
     1441 taccaatcgc cagtaacgca acgggagctg cacggttaag aatgtcgata gggctaccga
     1501 acaaacgccc atcctgaagc accacctgcc agaaatgcgg ggctaccagg ctatcgacca
     1561 gcagcaccag caacagtgcc gccagctgcg gcatccccgt cggccagtga aaacgccttt
     1621 ttggcggcgt agtgtcaggg agagattgag gcatcacact gttctcctta cgccgcaatg
     1681 gcgttcatga tcgccggaac ggaaagctgt gccagcggga tctccgccac ctgtttgcga
     1741 tcgcgcatga tgatcacccg gtcggcatag cccaccagct cttccagttc ggaggagatc
     1801 accagcagcg ccagaccgtc agcacacagc gtttcaatca ggcggatgat ctcggcgtgg
     1861 gcaccaacat caatgccacg ggttagctca tcaaggatca gaaattgcgg tcgggtcagt
     1921 agccaacgtg aaagcagcac tttttgctga ttgccaccgg agagaaattc aatcggttgt
     1981 tcagttgaag gtgtgcgaat gccaagctgg cggataaagc gttcggcaat ctcttgctgc
     2041 tctttgcggg aaatgggtcg tagccagccg cgctgggcct ggagagcgag gatgatattt
     2101 tcccgcaccg aggcggcggc gatgatgcca tcggttttcc tgtcttccgg gcagaatccg
     2161 atgcccagta ctgaagcctg atgtggcgat cgcagggttt gcggtttgcc tttgatcaac
     2221 gccgtgccgc tgtcagcagg tttgataccg aagatcactt cggcggtttc ggtacgtccg
     2281 gatcccagca agccagccag accgacgatc tcgccggggc gcacttcgag atcaaacggt
     2341 gctatcgttc cttttttgcc gtaatttttg aacgcggcaa cgggtttgtc gctcaacaat
     2401 gttcgcccgg cacgctgtag cgcgtgggta tccagctcgc gccccagcat catttttacc
     2461 agttcgatct gcggtagctc gcgcgtttcc cgacagccta cgaaactgcc gttgcgtaag
     2521 acggtgatcc gatcgctgac ctgatagacc tgatcgagaa agtgagtgac aaagatcagg
     2581 ctgacgccgc gatcgcgcaa ctgacgcatc aggccaaata atagctccat ttcctgggtg
     2641 tcgaggctgg cggtgggttc atcgaggatc agcactttgg cggagagatc gatagcccgg
     2701 caaatggcga cgatttgctg catcgccacc gaaaaacggt taagcggttc gcgcacgtcg
     2761 agggagaaac cgtaagatgc catcagttcg gtggcgcgct tttccatctc tttacggcgt
     2821 agaaggccga aacgtttggg ttcgcgacct atgaatagat tatcagcaac cgacatattg
     2881 ggtagcaggt tgacttcctg atagacggtg ccgataccga gttgttgtgc atgggcggta
     2941 tttttcggtg agatagtctg gccttccagc cagatggtgc cgcgatcggc gtggtataca
     3001 ccagttaatg ctttgattag cgttgatttt cccgccccgt tttcaccaag cagcgccatg
     3061 atttcgccac ggcgcaggct gaaatcaacg ttgtctaacg ctttgacgcc ggggaaaaat
     3121 ttacttaatc cttcggtgcg gaggatctcc tggtgttggt cggtcgtcat ggtttccccc
     3181 ggtgtaaagc gtgctcccag taggcatgat aagacgcggt aagcgtcgca tcaggcttca
     3241 gtgcaccatc gccggatgcg gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca
     3301 tagcaaccct caatacccca tatttttctt cttctctaac tcttcttttg cggtatcagg
     3361 caggtaaagg gtggatttgg ttaacgtcag cttttcaggc atggtgccgt cttttttgta
     3421 tttctccagc gcatcgaaag cgggtcccgc catgttcggc gtcagttcaa cactggcgtt
     3481 cgcttcgcca tccatcatcg ctttgtagat gtccggtaca ccgtcaatgg aacccgtgag
     3541 gatatctttg cccggtttca ggcccgcttc tttaattgcc tgaattgcac caatcaccat
     3601 gtcgtcgtta tgggcgtaaa ccatgcagat gtttttgccg ttgttttccg ctttgataaa
     3661 gctctccatg acttctttgc ctttactgcg ggtgaagtca cctgactgcg agcggataat
     3721 tttgatattt ggcgcattct taatggcttc ggcaaagcct ttcttacggt caatggcgac
     3781 gctggcccca acggtgccct gcagctccac cacgttgcat ggtttgccat tcacttcttt
     3841 taccagccag tcaccgatca acttaccttc gaggatgttg tcggcagtaa cggtggtcat
     3901 atagagagat ttgtctttca catcgatgga acggtcgagc aagaagaccg ggatttcggc
     3961 atctttcgcc tcttttaata ccggttccca acccgtcgca accactggag cgataaagat
     4021 cgcatccacc ccttatgcaa cgaaggaacg taccgcttta atctggtttt cctgcttttg
     4081 ctgaccatcg gcaattttca acgtaattcc gcgtttttcg gcctcacttt tcgccacatt
     4141 ggtttctgcg gcgcgccagc cggattccga tccgacctgc gaaaatccta cggttaatgg
     4201 agcggccaac gccatagacg acatggctgc cgagactgca gagactacaa gtaagcgttt
     4261 ccacataggg ttgtcctcgt cggggtaatt attattggtg aaaagatgtt cgcgaaaaaa
     4321 ctatagacaa ttcgttatgt aacggattgc gttacatcac acttcaaaaa agtaaataaa
     4381 acgttaatca caagtttgta atcgctttca tctcactatg aaaaatgcgg ctacggttat
     4441 ggattttcct gctctgtata ccgtcttaaa actggcgaaa aaggaaaatg aagacgaaaa
     4501 caagcaaaga cattcggcgc gagttggcta taatacttgg cacttgtttg ccacatattt
     4561 ttaaaggaaa cagacatgag cttactcaac gtccctgcgg gtaaagatct gccggaagac
     4621 atctacgttg ttattgagat cccggctaac gcagatccga tcaaatacga aatcgacaaa
     4681 gagagcggcg cactgttcgt tgaccgcttc atgtccaccg cgatgttcta tccgtgcaac
     4741 tacggttaca tcaaccacac cctgtctctg gacggtgacc cggttgacgt actggtcccg
     4801 actccgtacc cgctgcagcc tggttctgtg atccgttgcc gtccggttgc cgttctgaaa
     4861 atgaccgacg aagccggtga agatgcgaaa ctggttgcgg ttccgcacag caagctgagc
     4921 aaagaatacg atcacattaa agacgtaaac gatctgcctg aactgctgaa agcgcaaatc
     4981 gctcacttct tcgagcacta caaagacctc gaaaaaggca agtgggtgaa agttgaaggt
     5041 tgggaaaacg cagaagccgc taaagctgaa atcgttgctt ccttcgagcg cgcaaagaat
     5101 aaataagttc ttctagcgca ataaccctga acgccgggct tcggttagta agggtttttt
     5161 tatgcccgcg ataaataaac tctctattcc accatcatta ttctcagcgg ttgcaaggct
     5221 tgaacggtaa gaacaagcaa acccgaccac cattttgctg ttcatagcca cttgctggaa
     5281 gttagccgac ctcactcata ctcaccgttc aaagcgtgcc cccgttttac ccctgcacat
     5341 ttgtgaacgc cagcagagtt ttttatgcga ttgcagatat ggaaagcggc tcggagagaa
     5401 aagcaaaaga tggaaaagtt acaaaaaaaa atcgcgagat acatcgcata aaacaacgcc
     5461 acccgaagga ggcgtgcttt tattattact tatccctgtc taaccagtcg ccgctttcaa
     5521 ttagctttaa tccatcgacg ggtcgttgat aaacgtacat ccatgcactc ccatacggcg
     5581 tctgaatcaa ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca agttcggcca
     5641 gcgtggcgtt gtcaatacga taaacttcac cgtgtaccgt tccgttcccc ggaactgcgc
     5701 ctggatagtg gcccaggcta tacaactggt agttatcgat actgaaatcg cccagtaact
     5761 gggcattggt catccagtga ctgttgcctt gtttgtggcg taaactgccg tagacaaata
     5821 ttcgcattgc taaaactcga actgatagag caaatccagt gcctggtcta caccagacac
     5881 ggcttccaga tatagcttag gcatcaggcg ataacgtaac gtgagtgttg ctatagagtc
     5941 aaatataccc acgccgtatt tcacttgcag acctggcaat acatagccgc tgaccactac
     6001 ctgggaggag tcgcctactc ccagggtgtc gagcgctaaa ttgcttacgc caaacgtctc
     6061 gccgatttta cccacaatct ggccactttg cgcaaccccc agaccaatca gcatcgaggt
     6121 cattgccgca ctgtcgctct gatcgctctc cagcccttgt ccacgtagca aataagacaa
     6181 tgcagcttgt tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt cgtccgccag
     6241 accagtgacg cgaactccgg cgattacatc gtcttctgta gcatccgggt tacgaatagc
     6301 ttcaatatta agatacggtt gatccggcgg accagagaac agtaactcac ccttacgcac
     6361 aatcagatcc tgaccatagg catggaagcg cccttcaggg atgttgatct gcccgttcag
     6421 gcccagccct tgtttgtcct gtacgacgtt aagatcgccc gtcagccgcg ctttcaggcc
     6481 aaaggcgtca atgcgcacat tgttgccaac gtggacaatc aggttactgt taatcggaat
     6541 cgacgccgtt ttcggctctt ccggttgcag gttatcgtta agcatcacca catcgctgga
     6601 gacgcctact gcgctttccg gcagatcgtg caccacgatg cgcgcccacg ggacatccac
     6661 gcgaccatcg agggtaaaca ggtttggtgt agcctcgaat acaacatctg gcgatacatc
     6721 cattcgtacc atcggcggca cggtgatccg cactttactg cctttcgccg ttactcgtgc
     6781 ccgccagttt tcaatttggc tccagtcggc atcaccgttc aggtagattt caccctgctg
     6841 ggtccgtact gtaccggcaa gcgtcgagcg cataccgtta aagttgaccg caagctggct
     6901 cggctgcata tcaaacggca taaagttgcc gtcgatatcc acacccgtaa cctgaagctg
     6961 accaaacaac tgcgggcttt gcacatcacc acccagacgc aagttggcac tcaccatccc
     7021 cgctgctttt tccccacggg taaagatggg gtttatcatc gcaaggttga agttacggat
     7081 attgacgttg ccaccaagat tacggcggcc ttgcggatcg gtcacctgca cctgtccatc
     7141 aaactggccg ttattggtca ggcggatggt ccagcccaat tcggcacggt tgttacgcaa
     7201 ttccgccgtc agattcagtg tctgaaacgc caccggcagc gccgcatcgt tgacggtttg
     7261 cgttacctgc acgttacgcc ccgaaagggt gatactgccc tgcggcagcc cctctttcgt
     7321 ggtgtcccag gcaacatccg ctttacccgt gaagataccg ctggcctgag tggtttctgg
     7381 cataaacggt ttcagcatgg cgaggtcgaa gcggttgaga ttcaccaccg cacgcccttc
     7441 ggcccccgca tcgatagttt gcggcacgca cagttccgca ttcgggttaa gccagcagtg
     7501 tggcccgatg ctgatttttt gctccttatt gcggtaatcc agcgcaatat cgcgggtcag
     7561 cgaccacggg ccgaccggcg tctggaagcg ggtattgcta agagttccct tccagcgttc
     7621 ttctttgcga tcaaaacttc ctgccagatt aagctgcccg gagacaggct cgccctgaat
     7681 ccgcaactgt agctcgtgct gcttttcgct gcctttggca ttcagggtga cgaggttgat
     7741 atttacatcc ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg cgatctgatc
     7801 ggtggattta atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct gccagcgcag
     7861 gccgcgcgcg gtgatatctg ccagtagttg tggcgcttcc accgtgccgc gtactttcac
     7921 cagccctttc gctgtaccgc caagccccgg cagcgcgtta tccagccccg gcgcgttgat
     7981 ggtggcatca agattgagat cttttacccc cagctcgcct ttcacttcgg cactgtttgg
     8041 cccgagttcc agatgaagcc cagggatcat ccactgcata taactgttgc ctttcagcgt
     8101 gccgtcaacg ttcactttgt tctgtttgac gttaccggtc agcttcagtt ctggcacctc
     8161 catctgccag gtgccgccgt acaggctacc gcgggtttta atcaagccat tgagtttcga
     8221 cggccactcc gggaactctt tggcggtgtt aatgccatta agcgttagct caccgcgcca
     8281 actaatggcc tgctgccagt cgagcaacgc cttgagttca gttttccctt ccagcgccgc
     8341 gacggtgagt ttgtcgagat tgacctgctg ttcattacct ttggcatcaa gggtaatggt
     8401 ggctggcggg atctcctgtc ccttcactgc cgtacgcata gagagcgtgt aatcggtcat
     8461 tttgccggta agtttcagtt tcagatcatc cgcctgatac tgcttctcac cagtgaacgg
     8521 ccagtaaagc tgtttgctgt tcacttccac gttgagtggc aatccggctt ccgccagtcg
     8581 cgtctgggcg cgtaaatcca tatccaccgg accggaaagg ttaacgccta tctccagctg
     8641 ttcgcgcagc gcgccgccca ctttcagctt caccttttca cctttcaacg gctccacgtt
     8701 cagtgtgctg ttgagggtga tatccaccgg ccagttgtct gacagctgcg ccgtgccgct
     8761 ggcgttgacg atcccctggt tggaatcgat atccagggcg tccagtttag tattgccgtc
     8821 aatgctgctc actttcagca gcatggtgcg cacggtgatg tccgtgtcgc ccgtcacgcg
     8881 cagctgctcg cccttaaact cttcaatgtt caggttaagc ggcaaatgca cgtcggtcat
     8941 ttccggcaat accgggcgag aaaagagatc tttcagcgtt tcgccgagcg gcttttcctc
     9001 cggctgcgga ttttcaattt tcggttcgac cacttcttcc tgcgccactt ccaccacttt
     9061 cggcagagca atcagcaggc ctttcagcga cgtcggtttc agggtcaggg ttttctcctg
     9121 ccagttcagg ccggaggtga agtccatcac cgacaccgtg gtgtcatcaa tcttgatgtt
     9181 gacgttgtcc agcgccaccc gtgtcagggt gatgggatac ggcgtggaga gatccagcgg
     9241 accgctatct tcctcttctt caacctgttc agaaggaggc atttttttac tgtcgatgtt
     9301 gacctgaatg tctttcagcg ccaggtcatt aatacaaacg ctactgttcc acaggcactc
     9361 aagaccgacc cccagatgca gattacccgc ttttaccgcc acgcctagct gctcataacg
     9421 aacgtcagac aaggtgagat cgcgccagcc gccggtgacc ttgccaatat ccagtcctgg
     9481 cacccagcga tccgccgctt taaatacaag atgcaggccg ctggtggtgc ccaccagaaa
     9541 cgccaccgat cccagcaaca gtaagataac gataaccacg ccgaggctga tttttttcca
     9601 taaactcata attctggccc cagaccgatg taaaactgta acccgtgttc gtctttatcc
     9661 gcgaccggta cggcaaaatc gagtttgatt ggcccgaccg gcgattccca gcgtacgccg
     9721 accccggtac cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc gccactatcg
     9781 acaaacaccg cgccccacca ttttccggtc acgttgtact ggtattccag cgatccggtt
     9841 atcaacttcg aggccccttt caggtcaccg ttggcgtatt tcggggcgat agatttgtat
     9901 ttgtagccgc gaatactgcg atcgcccccg acgaagaaac gcagatccgg cggtactttg
     9961 tcgaaatcac cggtttcaat ccagcccagc gtgccgcgtg tcacaaagcg atggcgatcg
    10021 tacagtgtgc ggatccagac gtgctgcgcc tggaaaacgg agaaatcgac atctgaaccc
    10081 caggccgtgt tggagtagtc gatagagtag cgttgcgagt ctccccaggt tggcatcagg
    10141 ccaccacgag aacgcgtgcg gctaatcatc accccaggat aaaacagcat cgtggtgttg
    10201 gtaatttcac cctgggtaaa gtggtcgagg ctccagcgca ggttaatggc acgctgccag
    10261 ccgctggaga gatcccagta gcgagaagcc accagcgtag tggagtcaga ttcggtatcg
    10321 ttcaggtcag tgcgcttaaa accgccctgc accaaataat attgttccag tggattcttc
    10381 agcagcggca ttttatagct gaagtcgagg gtctgttccg gcgcggaaat actggtactg
    10441 gtggtcaaac tgtgaccgta tgagttcatc cacggctttt tccacgtcgc tttcacgcgc
    10501 ggtcccacgt ccgtagagta accgaccccg gtttcgatgg tgttttctgt tcgcggcgaa
    10561 accacgcccg tcaatggtaa tactttcgtt tcgcgcgctt tatcaaattg tggagccacc
    10621 accaccgagt taaaccagcc ggtagcagaa agtcggcggt tcagttccgc cagatctttc
    10681 gattcatact catcgccctc tttaaacggc accagatttt gcaggtattc atcgcggatt
    10741 tgtgatcctt caaaggtcac atgcccaaag cggtaacgtt cgccactgtt ataatcaata
    10801 tcccagaagg ctttatgcag gccgagcgca atgcccagct gcgctttggt aaattcgcta
    10861 tcgaaataac ctttacgcaa cgcaatactg gttaaggact ttttgaaatt ttcataatcg
    10921 ccctggttca gtaccgtacc aatagccggg cgagtatcga gcaatttcaa atagtcttta
    10981 tcggtccgcg cgccgccgcg caataccaca tcggtgccgc caattaacac cggcacacct
    11041 ggcgtgactt tggcgatcaa tacctgccgc cctttctttg gcggtggacg gagatcaaat
    11101 tcaatggtcg gctggtaata acccagcgct ttcagacctt cacggatggc atcatcgacg
    11161 cgtgcgcgaa agcgacggtc tggcgtcact tcatcacttt caatcgtaga aagctgcgca
    11221 cgaacgttct tttccagctg tcccgataac ccctcgacct gtagacggac gttcgcggcg
    11281 acggcagatc cgcttaagca gagtaagctt acacagcata actgtcggat atagcgcaca
    11341 ttttctcctg aatatccttt tttcctgccc ctggaacgcc gttaaacggc ttaacaaaaa
    11401 tccagtaata tggattaaaa agcagactaa accccaaata tttcttatgt tttactttag
    11461 acctattcac ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt aaccccaatg
    11521 accgattttc gggagagcga caccatgagt ttatttgata aaaagcatct ggtttccccc
    11581 gccgatgccc tgcctggacg taacaccccg atgcccgtag ccacgctgca tgcggtcaac
    11641 ggtcactcaa tgaccaatgt acctgacgga atggagattg ccatttttgc gatgggttgt
    11701 ttctggggtg tggagcgtct gttctggcag ttacccggcg tttacagcac cgccgcaggc
    11761 tataccggcg gctatacgcc aaatccgact tatcgggaag tgtgctccgg tgatacgggt
    11821 catgccgaag cggtacgcat tgtttacgat ccttccgtca tcagctatga gcagttgcta
    11881 caggtatttt gggagaatca cgatcccgcc cagggaatgc gtcagggcaa tgaccacggc
    11941 acgcagtatc gttcagcgat ttatccgctg accccagaac aggatgccgc agctcgcgcc
    12001 agtctggaac gttttcaggc ggcgatgctt gccgccgatg acgatcgtca catcaccacg
    12061 gaaatcgcta acgccacacc gttttattat gccgaagatg accaccagca atatctgcat
    12121 aaaaacccat atggttattg tggaattggc ggaattggcg tctgtctgcc gccggaagca
    12181 tagcgttacg ggtacaaatg tagtttgttg ataaagtgct ctttgtctat gcctgatgcg
    12241 gcttaaacga cttaccaggc ctacaaaatc gcccaaattc aatatattgc aaggactgcg
    12301 taggcctgat cggcatagcg catcaggcag tattgcattt atcagcagtc tgaattttaa
    12361 ccctctggcg actttacagt accttacgct atactagcca ctgaaaatgc cggttcactt
    12421 tcttcgaatc ggctttcaat gtgtatttca cacaaattaa tcaactttcc cttccgaggg
    12481 tctggcctga aaggtcggat aagatatgtt aaacagtatt ttagtcatac tctgcttgat
    12541 cgctgtaagt gcgttcttct cgatgtccga gatctcgctt gccgcctcac gcaaaatcaa
    12601 acttaaactg ctggctgatg aaggcaatat aaatgcccaa cgcgttctga atatgcagga
    12661 aaatcccggc atgttcttta ccgtggtcca aatcggtctg aacgcagtgg cgattctcgg
    12721 cggtatcgtc ggtgatgcgg cattttctcc agcttttcac agcctgttct cccgctatat
    12781 gtcggcagag ctctctgagc aactgagctt tattctctct ttctcgttag tgactggcat
    12841 gtttatcctg tttgcggatt taaccccgaa acgcatcggt atgattgcgc cagaagcggt
    12901 ggctttgcgt atcatcaacc cgatgcgctt ctgcctgtac gtttgcaccc cgctggtgtg
    12961 gttcttcaac ggcctggcga acatgatctt ccgtattttc aaactgccaa tggtacgtaa
    13021 agatgacatc acttctgatg acatctacgc ggtagtggaa gccggtgcgc tggcgggcgt
    13081 gttacgtaaa caggaacacg agctgattga aaacgtcttt gagctggaat cccgtaccgt
    13141 tccgtcttca atgacaccgc gtgaaaacgt gatttggttt gatctccacg aagatgagca
    13201 aagcctgaag aataaggtgg cggaacatcc gcactctaag ttcctcgtct gtaatgaaga
    13261 tattgaccac atcatcggtt atgtcgattc taaagacctg ctgaaccgcg tgctggctaa
    13321 ccaaagcctg gcactgaaca gcggcgtaca aattcgcaac acgctgattg tgccggatac
    13381 gttaaccctt tcagaggcgt tggaaagttt taaaaccgca ggtgaagact tcgcggtgat
    13441 catgaacgag tacgcgctgg tggtggggat catcaccctc aacgacgtga tgaccacgct
    13501 gatgggcgat ctggtcggtc aggggctgga agagcagatt gtcgcccgtg atgagaactc
    13561 atggctgatt gacggcggca ccccaattga cgacgtcatg cgcgtgctgg atattgacga
    13621 gttcccgcag tcgggcaact acgaaaccat cggcggcttt atgatgttta tgctgcgtaa
    13681 gatcccgaaa cgcaccgatt cggtgaaatt cgccggctac aaatttgaag tggtggatat
    13741 cgataactac cgcatcgacc agctgctggt gacccggatc gacagcaagg ccaccgccct
    13801 ttcgccaaaa ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac ccccagaaac
    13861 atcaacggct cctgaatcag gagccgtctt attactgcat agcactttgg ttaagccatc
    13921 tctgtttgca gacgcataac ctgacggtta acttcggaca tcactgacaa atgcagctta
    13981 tccttcactt ttgggataag aatcttaccc ttatcaaatt caaaagcgcc aatgtccttg
    14041 atgtataacc gtccacggaa caggatcttt acgtacttcg ccacctgaag tgggttgtac
    14101 cgttggaaaa ttttcattgt tatctcctgc tgagtattac gcctgtgcgg tgccacaatc
    14161 ggcccaacta ttatgaggcg caaattttaa tgcttagtga ctatagacta tccgggcaat
    14221 gtttccaccg cgtataactt tttttaccta aaggttacaa ttattcagaa ttatcttttt
    14281 accgaagcgc ggtcttcagt ataagcattc atttttcata tgatttgtgc gcttgaccgc
    14341 aaactggcat cacacttgcg ggaaattcga taaatagcac atatgattaa aactcagacc
    14401 caagtggtcg gatcacctgc atatcataag aaggaaacac catgacccta cgcaagattc
    14461 tggcactcac ctgcctgctg ttgccgatga tggcttccgc acatcagttc gaaatcggtc
    14521 agcgagtgcc gccgattggc attaccgatc ggggcgagtt ggtgcttgat aaagatcagt
    14581 ttagctacaa aacctggaac agcgcgcagt tagtgggaaa agtgcgagta ctgcaacata
    14641 ttgctggtcg cacctctgca aaagagaaaa acgcgacgct gattgaagcg attaaatcag
    14701 cgaagttacc gcacgatcgt taccagacca ccaccattgt taacaccgac gacgcgattc
    14761 cgggttccgg catgtttgtg cgcagcagtc tggagagtaa taaaaagctt tatccctggt
    14821 cgcagtttat tgtcgatagc aatggcgtcg cgcgcggtgc ctggcagctg gatgaagaaa
    14881 gttccgctgt agtggtgctg gataaagacg gtcgcgtgca atgggccaaa gacggggcgc
    14941 tcacaccgga agaggtgcag caagtgatgg acctgctgca aaagttgctc aaataatggc
    15001 aataacataa attctggaaa aataattttc ccctatcgtt atctatggga taggggaatt
    15061 aagtctctga gttcttacac cgaactaata ctggtaacgc tagttgctct tttttaaacg
    15121 cctcaatggt cgatttatat ttctcgcaaa tcttttcttt ggtaatatta ctatctgcgg
    15181 ccagattcgc acttactatc gcatccactt gttcggctaa ttcgacttgt tggcaacctg
    15241 cattaaccag aaaattataa ataagagagt aatcttcatc ctccaaaaca acagaggaac
    15301 cattacgaaa acgttcatcc cctccataga ttttaagcgc aaagcccaaa gattctatga
    15361 ctatggcaaa attatcatca attatttttt cgtaaatata ccatccatcg cattctttaa
    15421 ttccaccgcc tggtatttgg agttcaaatt taccccgttt taatttgctt actacatcct
    15481 ccaataaggc ataaccgaaa gatacgtcac caaacgcctg gcgaatataa cctttaatcc
    15541 tttttggtat gtcagactgt tttttctgca aaatatcaca ggcatacaat cttctgtttt
    15601 gttctaacgg aaactcgcgg tttttgaaat ccatgaatga gttatactca tttattgtta
    15661 tgggaatttc ttcgtattca aacccactgc ctcgcaatgt tattgtatat tcagcaccgt
    15721 tatctttaag gctgaaactg ataagaaaat tatccatggc attaacagtt tcattttttg
    15781 atgaaagcac ttgataacta tttttatcaa tctgtgttgc gcaacgaaag gactcccgca
    15841 aagttaacag actatcataa ttcgaaaccc aatctttttt cttaaatgca tgctgaatat
    15901 tattaaaaaa atcgtttgaa tgtgtttttg tatgcgatga ctttacaaat aactccaccg
    15961 caccaataaa attattgtgt ctgatcctgg gtaacatatg gctaccttat tttatttggt
    16021 tattttatgt cgccattaaa tatctcatta tagaagtaaa ctatcaaaaa acgctcatta
    16081 ttaaaatgta acttactagc aattgaattt ttgcacttta aatacaatct ctttttattt
    16141 ataacttttt aggttgggat aaatcaggat ggcagaatca ggaaatacac tgtttctgcc
    16201 atctgaattt agtaaatgga cactctgaac cccggattca ggaacgactc gcgcggagtg
    16261 taatccagcg gtttaccctg ccagtcgtga acgtgcgctc cggcagctgc agccacagca
    16321 tgtccggcgg cggtgtccca aatattcgtt ggtccgaagc gcgggtacag ctgcgcctgt
    16381 ccttccgcca ccaggcagaa tttcagcgaa gagccgatgg acgtggtctg atgttcgcca
    16441 agctgttgca gatactcttt cagctccgca tctgcatggg aacggctgat aaccaccagc
    16501 ggcgggcgcg catcgcggac ctgaatcagc ttgcgcacac cgcactcttc tttccaggcc
    16561 ttgccttctg ccgcgctgta cattacgttc attaccggcg catacaccac acctaaaatc
    16621 ggtttgccat ggtcaatgag cgcaatgtta acggtgaatt cgccattacg tttaataaac
    16681 tctttagtac catccagcgg gtctaccagc cagtaacgct gccagtgctg acggacttcc
    16741 caaccgggag gatcttcttc agaaaggacc ggaatatccg gtgccagcgt acgtaaaccg
    16801 tccatgataa cggtgtgagc ggcaatatcc gctgccgtta ccggagaatt gtccgctttg
    16861 ctgacgacgt ccatcggttt cgtcccgtcg tagacctgca taatggcatc gcctgcattc
    16921 cgtgcaagct ggcatacttg atctaacatt tctccacctc gtctctgtga gcggtgttaa
    16981 cttattgttt tacttatacc ctatcgttaa tgaatgcgcc aactgtgata gtgtcatcat
    17041 tttcaaagcg taaaattgtg gcattcttca ctgttctata agtaaggcgt ttattctccc
    17101 ttttctttcg tattcccgat gataaaagga tgtccctgat gattaagttt agcgcaacgc
    17161 tcctggccac gctgattgcc gccagtgtga atgcagcgac ggtcgatctg cgtatcatgg
    17221 aaaccactga tctgcatagc aacatgatgg atttcgatta ttacaaagac accgccacgg
    17281 aaaaattcgg actggtacgt acggcaagcc tgattaacga tgcccgcaat gaagtgaaaa
    17341 acagcgtact ggtcgataac ggcgatttga ttcaggggag tccgctggcc gattacatgt
    17401 cggcgaaagg attaaaagca ggtgatgttc atccggttta taaggcgctg aatacgctgg
    17461 attatacggt cggtacactc ggcaatcatg aatttaacta cggtctggat tacctgaaaa
    17521 atgcgctggc gggagcgaaa ttcccttatg taaatgccaa cgtcattgac gccagaacca
    17581 aacagccaat gtttacaccg tatttaatta aagataccga agtggtcgat aaagacggaa
    17641 aaaaacagac gctgaagatt ggctatattg gcgtcgtgcc gccgcaaatc atgggctggg
    17701 ataaagctaa tttatccgga aaagtgacgg tgaatgatat taccgaaacc gtgcgcaaat
    17761 acgtgcctga aatgcgcgag aaaggtgccg atgtcattgt cgttctggcg cattccgggc
    17821 tgtctgccga tccgtataaa gtaatggcgg aaaactcagt ttattacctc agtgaaattc
    17881 cgggcgttaa cgccattatg tttggccatg ctcacgccgt tttcccgggt aaagattttg
    17941 ctgatatcga aggggctgat atcaccaaag gcacgctgaa tggtgttccg gcggtaatgc
    18001 cgggcatgtg gggcgatcat cttggggtgg tcgacttaca actcagtaat gacagcggta
    18061 aatggcaggt gacgcaggcg aaagcggaag cacggccgat ttacgacatc gccaataaaa
    18121 aatccctcgc ggcggaagac agcaagctgg tagaaacact caaagccgat cacgatgcca
    18181 cacgccagtt cgtcagcaag ccaatcggta aatctgccga caatatgtat agctatctgg
    18241 cgctggtgca ggacgatccg accgtgcaag tcgtgaacaa cgcgcaaaaa gcgtatgtcg
    18301 aacattacat tcagggcgat ccggatctgg caaaactgcc ggtgctttca gctgccgcac
    18361 cgtttaaagt tggtggtcgc aaaaatgacc cggcaagcta tgtggaggtg gaaaaaggcc
    18421 agttgacctt ccgtaatgcc gccgatcttt atctctaccc caatacgctg attgtagtga
    18481 aagccagcgg taaagaggtg aaagagtggc tggagtgctc cgccggacag tttaaccaga
    18541 ttgctcctaa cagcacgaaa ccacagtcac tcatcaactg ggatggtttc cgcacttata
    18601 actttgatgt tattgatggt gtgaattatc agattgatgt tacccaaccc gcccgctatg
    18661 acggcgagtg ccagatgatt aatgccaatg cagagcggat taagaacctg acctttaatg
    18721 gcaagccgat tgatccgaac gctatgttcc tggttgccac caataactat cgcgcttacg
    18781 gcggcaaatt tgccggtacg ggcgacagcc atatcgcttt tgcttcaccg gatgagaacc
    18841 gctcggtgct ggcagcgtgg attgctgatg agtcgaaacg tgcgggggaa attcatccgg
    18901 cggcagataa caactggcgt ttagcaccga tagctgccga taagaaactg gatatccgtt
    18961 tcgaaacttc cccgtcagat aaagccgcag cgtttattaa agagaaaggg cagtatccga
    19021 tgaataaagt cgcgaccgat gatatcgggt ttgcgattta tcaggtggat ttgagtaagt
    19081 aaaacacttc tttttcggcc tataaatcaa ccgcatccgg catttattgg cggatgcgat
    19141 gctgccgtat cttatccacc ctacaagcca tgaaccgtag accggataag cgcagcgcat
    19201 ccggcagtta tgccgcacgt tcatcccgca ccgccaacac ctcgggcaaa ttcaactcaa
    19261 tccagtccgc cagtgcagca accttttcgc tcacctgctc gcccagcggc gtgaggctat
    19321 attccacatg cggcggcacc accggatacg cgatacggtt aagaaaacca tcctgttcta
    19381 acgcctgtaa cgactgcgca agcatctttt cactcacccc accgattttg cgccgcaggt
    19441 cgctaaagcg atgagtacct tcgcgtagcg ccaccagaat caacaccccc caacggctgg
    19501 tgacgtgttt caacacctcg cgcgacgggc actgttccgc aaagagatta ccctctttca
    19561 gttgttgtga caggctaacc tgactcattt catacttacc tttttgcacg tacttactaa
    19621 aagtaagttt aggtgttagc gtatttaaac acaagacaaa acgatggaga cttcccatga
    19681 tcgctattac tggtgccact ggccaacttg gtcactatat tattgaatcc ttgatgaaaa
    19741 cggttcctgc cagccaaata gtggctatcg ttcgtaatcc ggcaaaagcc caggccctgg
    19801 cagcacaagg cattaccgtg cgtcaggctg actacagcga tgaagccgca ctgacatctg
    19861 cacttcaggg agtggaaaaa ctactgctga tctcttccag cgaagtgggt caacgtgccc
    19921 cgcagcatcg taatgttatt aatgccgcaa aggcggctgg cgtgaaattt atcgcttata
    19981 ccagcctgct acatgcagat acctccccgc tcggcctcgc cgatgagcac atcgagacgg
    20041 agaaaatgct ggctgattct ggcatcgttt acaccctgct gcgcaacggc tggtacagcg
    20101 aaaactacct cgccagcgcc ccggcagcac tggaacacgg cgtatttatc ggtgcggctg
    20161 gcgatggcaa aatcgcctca gcaacgcggg cagattatgc gacagctgcg gcacgcgtga
    20221 ttagcgaagc cggtcacgaa ggcaaggttt acgaactggc gggcgatagt gcctggacgt
    20281 tgacacagtt agcggcagag ctgaccaaac agagcggcaa acaggttacc tatcaaaatc
    20341 tgagcgaagc cgatttcgcc gcggcactga aaagcgtcgg actgcccgac ggactggcgg
    20401 atatgctggc ggattctgac gttggcgcat cgaaaggtgg tctgtttgat aacagcaaaa
    20461 cgcttagcaa attgattggc tgcccaacga caacgttagc cgaaagcgta agccatcttt
    20521 ttaatgttaa taactagtta attaaagtgg catcctcccg catcctctct gataatgacg
    20581 ggatgccggg agcaatcatg tctgcttcct gaacgtttct tctgacagac caatggatgc
    20641 cagtaatgat tagcggcgtg ctgtacgccc tgttagcagg gttgatgtgg gggcttattt
    20701 ttgtcgggcc gttgatcgtg ccggaatacc cggcgatgtt gcagtcgatg gggcgttatc
    20761 tggcgttagg gttaattgcg ctacccattg cctggctggg acgcgtgcgt ctgcgtcagt
    20821 tggcgcgtcg ggactggctt accgccttga tgctcactat gatgggcaac ctcatttatt
    20881 acttctgcct tgccagtgcc attcaacgta ctggcgcgcc tgtttccacg atgattatcg
    20941 gcaccctgcc ggtggtgatt cctgtctttg ccaatctgct ttatagccag cgcgacggca
    21001 aactcgcgtg gggaaaactc gccccggcac tgatttgtat tggcatcggc ctggcgagtg
    21061 tgaatattgc tgagttaaac cacggactcc ccgattttga ctgggcacgt tatacctctg
    21121 gcatcgtgct agcgttagtt tccgtggtct gctgggcatg gtatgccctg cgcaacgccc
    21181 gctggctgcg ggaaaatccc gacaaacatc cgatgatgtg ggcgacggcg caggcgctgg
    21241 tcacgctgcc ggtttctctc atcggctatc tcgtcgcctg ttactggctg aatatacaaa
    21301 cgccggactt ctccttacct tttggccccc gtccgctggt gtttattagt ctgatggttg
    21361 cgatagccgt gctttgctca tgggttggcg cactctgctg gaacgtcgcc agccagcgat
    21421 taccgacagt gattctcggg ccgctgattg ttttcgaaac gctggcaggt ttgctgtaca
    21481 cctttttact ccgccagcaa atgccgccgc taatgacgct gagcggtatc gcgctgttag
    21541 tgattggcgt ggtcatcgcg gtcagagcaa aaccagaaaa acctttaact gaatctgtct
    21601 cagaaagttg acatgctggc agtgagttaa ataagcctct gctacgtaag ggttatagct
    21661 tttgccttaa aggtgtattt aaaatacatc ttatcttatt aagaatgagg tatcagctat
    21721 ggcttatcgc gaccaacctt taggtgaact ggcgctctct atccctcgcg cttcggctct
    21781 gtttcgtaaa tatgatatgg attactgctg tggcggtaag cagacgctgg cgcgcgcggc
    21841 ggcacgtaaa gaactggatg ttgaggtcat tgaagctgaa ctggcaaagc ttgctgaaca
    21901 accgattgag aaagactggc gtagcgcccc gctggcagaa atcattgacc atatcatcgt
    21961 gcgctaccac gatcgtcacc gcgagcagct gccggagctg atcctgcaag cgactaaagt
    22021 cgagcgcgtt cacgccgaca aaccgagcgt gccaaaaggg ctgacaaaat acctgaccat
    22081 gctgcatgaa gagctttcca gccacatgat gaaagaagag cagatcctct tcccgatgat
    22141 caaacaaggc atgggcagcc aggcaatggg gccaatcagc gtaatggaaa gcgagcacga
    22201 tgaagcgggc gaactgctgg aagtgatcaa acacactacc aataacgtca caccgccgcc
    22261 agaagcctgc accacctgga aagcgatgta taacggcatt aatgaactga ttgatgacct
    22321 gatggatcac atcagtctgg aaaacaatgt actgttccca cgcgccctag cgggtgagtg
    22381 atctaaaagc tggatggcat tgcgccatcc agcatgataa tgcggttatt tccgcagttc
    22441 agcagcccgc ttcttaccaa taaacagcca gcccaacccc agcgcgataa accacagcgg
    22501 ggtaaccagc agcgcctggc gagtgtcatc ttccagtgtc agcaacacga ccacgaacac
    22561 aaagaacgcc atacataccc agcacatcag cttgccgagc ggcatcttgt agatcgactt
    22621 ttcatgcaga tgaggacgct gtttgcggta caccaggtaa gagcaaagga taatcgtcca
    22681 gacgaacata aacagaatcg cggaaacggt tgtaatcatc gtgaacgcgc caatcacgct
    22741 aggattcaca tacaacatca ccacgccgcc gagcagacag atacacgaga aggtcaaccc
    22801 tttcgcgggt actgcgcgct tagaaagttt agcgaacgct ttcggcgcaa caccttcctg
    22861 cgccagacca aacagcatac ggctggtaga gaagacaccg ctgttagcgg aagacgccgc
    22921 agaggtcagc accacaaagt tgatcacgct ggcagccgca ggcaaaccta ccaacacaaa
    22981 cagctcaacg aacgggcttt tctccgggac taccgaactc cacggtgtca cggacataat
    23041 cacaatcagc gcgaagacgt agaacataat gatacggatc ggaatggagt taatcgcgcg
    23101 tggcagtgat ttctccggat ctttggtttc cgcagctgtt gtacctacca gctcaatccc
    23161 cacgaaagcg aaaaccgcta tctggaatcc ggcaaagaag ccacttaaac ctttcgggaa
    23221 ccagccgccg tcattccaca aatgcgcgaa tgacgcttcc acaccggtcg gtgactgaaa
    23281 gtgcatcgcc accatgacca ggccgacgac aatcagcgac acgatggcga cgattttgat
    23341 catcgcaaac cagaactcca tctcaccgaa cattttcacg gtggcaaggt tgagcgtcag
    23401 cagcagcact atcaccgcca gcgaggcgac ccagtcggag agatcgggga accagaactg
    23461 agcataagcc gtgatcgcca ccacgtctgc cataccggtt acaacccagc agaaccagta
    23521 agtccagccg gtgaaatatc ccgcccacgg ccccagtaaa tcggaagcga agtcactaaa
    23581 agatttgtat tccagattcg aaagcagcaa ttcccccatt gcccgcatca cgaaaaagag
    23641 cataaaacca atgatcatat aaacgaaaat gatcgacggc ccggcaaggc taatcgtttt
    23701 gccggacccc ataaacaacc cagtaccaat ggcacctcca atggcaataa gctgaacatg
    23761 tcggtttgtg agattgcgcc gtagcgactg ttcagccgga gcctgatcat cggcaacgac
    23821 ttttacctga tctaccatgt tttttcttcc tgtacctgtc tgtgttgttc aggctctacg
    23881 gcctttagtc agtctatgat acgacgaatc tgtatcggga ctcatcgata ttaggtaaca
    23941 atacgcggga tgaatactaa gatttagatc taatgttaat tttatgttta aagtgagtgt
    24001 catatcacgg tgataacgcg aaacagctca caaaaataca cgcttacggg gtctttgcaa
    24061 cacaaaatag cgccgtttgg caaactgaag ggtttattgc tgaatgcctg ctcccctctg
    24121 gttcgagggg aacagaatgc gctgcttaga ggatttccag cagttcgact tcaaacacca
    24181 gggtgctgaa cggagggatg gatgcacctg cgccgcgctc gccatatgcc agttcctgcg
    24241 ggatagtcag ttcccattta gaacctaccg gcatcagagt cagtgcttca atccagccag
    24301 ggatcacgcc attaaccggg aattcagcgg gttcaccacg agcaacggag ctgtcaaaca
    24361 cggtgccgtc gatcagttta ccggtgtaat gaacacgaac gcggtcggta cgtgccggaa
    24421 ttgcgccttc accctggttg atcacgcgga attgcaggcc agattcggtg ctattcacac
    24481 cttctttttt ggcgttttct tccaggtatt tcacgccttc agcagccatc gcctggaaac
    24541 gctgacgacg aacggcatcg gcgcgttcgt ggatttcacg cagcgcgcga tgcaccacat
    24601 caaccggcac agccggatgt ttgccttcca gcgcatcggc aatacctgca accagtgctt
    24661 ctggcagcag cccttccagg ccagattcac tcagttgttg ccctacctgc aaaccaatgc
    24721 cgtagcttgc ttgcgcttcg atggtgtcaa aagttggggt ggtcatcgta tttcctttct
    24781 tagggtataa aagtagcggg cagcatatca gccacacttc tttgggtaaa actttgtcgc
    24841 aggaatgatg acaaatcccg gggaaaaaga aacaatagca atatcccgta tgaatataat
    24901 aaaggcgggt ttaccacgga catcatagcg ttagcttatt cataggctat gattgaggaa
    24961 caagacgtgg agcaggagga aaccatgccc gggcgctttg aactaaaacc aaccctggag
    25021 aaagtctggc acgcgccgga caattttcgc ttcatggacc cgctgccgcc gatgcatcgt
    25081 cgggggatca ttattgccgc cattgtattg gtggtcggat ttctgcttcc atctgatgat
    25141 acgcccaacg cgtcggtggt gacgcgtgaa gcgcagttag atattcagtc gcaatcacaa
    25201 ccgccgacag aagaacagcc gcaaactacc caaacgcaac cattccagcc agatagcgga
    25261 attgataatc aatggcgctc ttaccgtgtc gaacccggta aaacgatggc gcagctgttc
    25321 cgcgaccacg gactgcccgc taccgatgtc tatgcaatgg cgcaggtaga aggcgcgggt
    25381 aagccgctga gtaatttgca aaatgggcag atggtgaaaa tccgccagaa cgccagcggc
    25441 gtggtgacgg gcttaaccat tgatacgggg aataatcaac aggtcctgtt tacccgccag
    25501 ccggatggca gttttattcg tgcgcggtag atgaagggtc aaccaaaagc cagctaagga
    25561 ttgtctcttt gatgacctta cgtctggctt ccagtgcctg gggggaaacc atactgatat
    25621 gataaactag gccaagcgta tgctggttga tcaaataata tcccccaagc gcagcaatat
    25681 taatattcac ctgcaatggg tcgatacctg gcttaaaaat attgtatttc ttcccttcat
    25741 ccaataagga ttccattaac tgcaagtgag catggttaat ttctaagaga cgctgtgatt
    25801 ttgcataatg cacaccttta ctttgatttt cactatgcac aatttttaaa aaccaaggat
    25861 tagcaatata ataatcccat gtaaaatcaa taattttctc aatggctttt tgtggcggaa
    25921 gatctgagaa atcaagggcc ttttcttttt gtcgaatatc gttccaggta tattccagca
    25981 cttcgataaa taaattttct ttattaccaa agtggtgata aaccgtctgt ttactacacc
    26041 ctgctgcctt tacaatatta tctactctgg ccccttcata accatattcg gcaaattcat
    26101 taaccgcaca tagcagcaat ttttccttca ggctctgtac gttgctggta tcaatataca
    26161 taacgcttcc ccacgacctc ttcgtggtga ctatcataac ctgatttgat ttcagcttca
    26221 gaaaaaaggg caaaaacttg tactttcatc aggttatcaa atgtcctgtg atcgccatcg
    26281 catatcaacc aactagatgg tttatctttt tatcctccag tcgcaagcta atcatcacca
    26341 aggaaacaaa attcaaaaaa gagagctatt gtatgaaatc cactcgtcct gttgccgtga
    26401 ttaccggagc cactcgcggt attggaaagg gctgtgcgca ggagcttgct cgtggcgggt
    26461 ttaacctgct gattaacgat cgtcccgatg ccgacagcgt agaaaaactg catatcacac
    26521 aacaagaatg tttggcggaa ggcgtggaag tgatttgctt ccctgctgat gtcggtgatc
    26581 tatctctgca tgaggagatg ctcgacgcgg cgcaaaacca gtgggggcgg ctggactgtc
    26641 tgctcaataa cgcggggatt tcagtgaaaa aacgcggtga cctcctcgac ctcgaaccag
    26701 acagcttcga tcaaaacatt gccattaaca cccgcgcgcc gttcttcctt gctcaggcat
    26761 tcagtaagcg cttactggca cagccgaaac cggaagcgga gctgccgcac cgctcgatta
    26821 ttttcgtcag ttccatcaac gccatcatgc tggcgatgaa tcgcggcgaa tacaccattg
    26881 ccaaaaccgc cgtttctgcc gctgctcgtc tgtttgctgc tcgcctttgt aatgaacaaa
    26941 ttggcgttta tgaagtgcgc cctggtctca tcaaaactga tatgactatt ccggcaacgg
    27001 cttattacga cgaactgatt gccaaaggtc tggtgccgtg gggacgttgg ggttatccgg
    27061 ctgatattgc ttccaccgtc cgcgcgatgg cggaaggtaa actgatttac acctgtggtc
    27121 aggcggtcgc aatcgacggc ggcttaagta tgccgcgctt ctgaggctga atcatgagag
    27181 atctgttaca acgcccggat ttattcagta tcaataccgc gacactgggc tataaaacgc
    27241 cgctacccgc cattattgat gcctgcgccg cacgcggtat tggcgcaatt gccccctggc
    27301 gcagggagtt gcagggtgaa gatctccagc aaatcacccg ccagcttgcc gccagtaaca
    27361 tgagcgtttc cggtttgtgc cgtagcactt actacaccgc gccgacactg gcggaacgca
    27421 aactggcgat tgatgataac cgccgggcgc tggacgacgc agcggtgctt aacgccgcct
    27481 gttatatgca ggtggttggc ggtctgccaa cgggcactaa agatctatat gaagcgcgag
    27541 aacaagtgaa acaagggatt cgccagttgc tgccacattc gaaagatgtc ggcgtgccaa
    27601 ttgcgctgga gccactgcac ccgatgacag ctgccgatcg ctcttgcctg tgcacgttac
    27661 gtcaggcgct ggactggtgt gatgaactcg atcccgatgg tgagtttggc ctcggcgtgg
    27721 cggtggatgt ttatcacgtc tggtgggatc cggatctcgc cagtcagatc ctgcgtgccg
    27781 gaaaacgcat cctcgcgttt catgtttccg actggt
//
LOCUS       DAJTYH010000054        27381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTYH010000054 DAJTYH010000000
VERSION     DAJTYH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..728
                     /locus_tag="OO609_002225"
     CDS             <1..728
                     /locus_tag="OO609_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6633629.1"
                     /translation="CGLFLLQHGAESALVDELSSRLGRALGMDSVESSISSNAIVLTT
                     IKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGVEKRFSQIQPLRYPRW
                     LVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQRHLHPQINFCLTAFA
                     ATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMFKGHINTGLARWAIAS
                     LLTLATCVGVVMALTIWGLRGWV"
     gene            719..1192
                     /locus_tag="OO609_002226"
     CDS             719..1192
                     /locus_tag="OO609_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6633630.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1299..1838
                     /gene="dnaT"
                     /locus_tag="OO609_002227"
     CDS             1299..1838
                     /gene="dnaT"
                     /locus_tag="OO609_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6633631.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            1841..2578
                     /gene="dnaC"
                     /locus_tag="OO609_002228"
     CDS             1841..2578
                     /gene="dnaC"
                     /locus_tag="OO609_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6633632.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2627..3121
                     /locus_tag="OO609_002229"
     CDS             2627..3121
                     /locus_tag="OO609_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6633633.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3361..5652
                     /gene="opgB"
                     /locus_tag="OO609_002230"
     CDS             3361..5652
                     /gene="opgB"
                     /locus_tag="OO609_002230"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6633634.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(5790..6812)
                     /gene="lgoD"
                     /locus_tag="OO609_002231"
                     /pseudo
     CDS             complement(5790..6812)
                     /gene="lgoD"
                     /locus_tag="OO609_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            6951..7865
                     /locus_tag="OO609_002232"
     CDS             6951..7865
                     /locus_tag="OO609_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6633635.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8080..9441
                     /locus_tag="OO609_002233"
     CDS             8080..9441
                     /locus_tag="OO609_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633636.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9490..11154)
                     /gene="tsr"
                     /locus_tag="OO609_002234"
     CDS             complement(9490..11154)
                     /gene="tsr"
                     /locus_tag="OO609_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6633637.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEISSASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11273..11719)
                     /gene="hpaR"
                     /locus_tag="OO609_002235"
     CDS             complement(11273..11719)
                     /gene="hpaR"
                     /locus_tag="OO609_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6633638.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            11991..13280
                     /locus_tag="OO609_002236"
     CDS             11991..13280
                     /locus_tag="OO609_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6633639.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13277..14743
                     /gene="hpaE"
                     /locus_tag="OO609_002237"
     CDS             13277..14743
                     /gene="hpaE"
                     /locus_tag="OO609_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6633640.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            14745..15596
                     /gene="hpaD"
                     /locus_tag="OO609_002238"
     CDS             14745..15596
                     /gene="hpaD"
                     /locus_tag="OO609_002238"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6633641.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15606..15986
                     /locus_tag="OO609_002239"
     CDS             15606..15986
                     /locus_tag="OO609_002239"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6633642.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16054..16857
                     /gene="hpaH"
                     /locus_tag="OO609_002240"
     CDS             16054..16857
                     /gene="hpaH"
                     /locus_tag="OO609_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6633643.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            16868..17671
                     /gene="hpaI"
                     /locus_tag="OO609_002241"
     CDS             16868..17671
                     /gene="hpaI"
                     /locus_tag="OO609_002241"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6633644.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            17693..19048
                     /gene="hpaX"
                     /locus_tag="OO609_002242"
     CDS             17693..19048
                     /gene="hpaX"
                     /locus_tag="OO609_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6633645.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19058..19948
                     /gene="hpaA"
                     /locus_tag="OO609_002243"
     CDS             19058..19948
                     /gene="hpaA"
                     /locus_tag="OO609_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6633646.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            19975..20912
                     /locus_tag="OO609_002244"
                     /pseudo
     CDS             19975..20912
                     /locus_tag="OO609_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21139..22697
                     /gene="hpaB"
                     /locus_tag="OO609_002245"
                     /pseudo
     CDS             21139..22697
                     /gene="hpaB"
                     /locus_tag="OO609_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            22715..23227
                     /locus_tag="OO609_002246"
     CDS             22715..23227
                     /locus_tag="OO609_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6633647.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23620..25770
                     /gene="btsT"
                     /locus_tag="OO609_002247"
     CDS             23620..25770
                     /gene="btsT"
                     /locus_tag="OO609_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6633648.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25820..26023
                     /locus_tag="OO609_002248"
     CDS             25820..26023
                     /locus_tag="OO609_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6633649.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26034..26990
                     /gene="yjiA"
                     /locus_tag="OO609_002249"
     CDS             26034..26990
                     /gene="yjiA"
                     /locus_tag="OO609_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6633650.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27059..>27381)
                     /locus_tag="OO609_002250"
     CDS             complement(27059..>27381)
                     /locus_tag="OO609_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6633651.1"
                     /translation="TYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGNLTLIS
                     GSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEWGLAELKMRHEKMKTQLKK
                     LWLV"
BASE COUNT         6609 a         7215 c         6971 g         6586 t
ORIGIN      
        1 agtgtggatt atttcttttg caacatggtg cggaaagcgc gttggttgat gagctttcct
       61 cacgactggg tcgggcgctg ggaatggaca gcgtcgaaag ttctatctct tcgaacgcca
      121 tagtgctgac aactattaaa gatgggcaat gcctgacatc gacacgtaaa aatcacgatc
      181 gcggcattaa tatgcatgtg gtgactgaag tccagcacat tgtgattctt gcggagcatc
      241 atctgctgga ttacaaaggc gtagagaaac gatttagcca aattcaacca ttacgttacc
      301 caagatggct ggtagcctta atggttggcc tttcttgcgc ctgtttctgt aaactcaata
      361 acggtggctg ggatggtgcc gtcatcacct tctttgccag tacgaccgcg atgtatatcc
      421 gccagctgct ggcacaacgt catcttcatc cacagatcaa cttttgcctt accgctttcg
      481 ccgccaccac catttccgga ttgcttttgc aactccccac tttcagcaat acccccacca
      541 ttgcgatggc cgccagcgtt ctgctgctag tgccgggctt tccgttgatt aatgccgtcg
      601 ccgatatgtt taaaggccac atcaataccg gactggcacg ctgggcgatc gccagtctgc
      661 tgacactggc tacctgcgtc ggcgtagtga tggcactgac gatttggggg ctacgcggat
      721 gggtgtgatc gaatttctgt tagcgttggc gcaggatatg atcctcgccg ccattcctgc
      781 ggtcggcttt gcgatggtgt tcaacgttcc cgtgcgggcg ttacgctggt gtgcgctgct
      841 tggctcgata ggtcatggtt cccgaatgat cttgatgacc agcgggttga atattgagtg
      901 gtcaaccttt atggcttcta tgctggtcgg taccattggt attcaatggt cgcgctggta
      961 tctggcgcat ccgaaagtgt ttaccgtggc ggccgttatc cctatgttcc cgggcatatc
     1021 ggcttatacc gcaatgattt cgtcggtaaa aatcagccag ttaggttaca gcgaaccgtt
     1081 gatgattacc ctgttaacca actttcttac agcttcatcg attgttggtg cgttatccat
     1141 cggtctttcc attcctggat tatggttgta ccgcaagcgc cctcgcgtat aaaattgcct
     1201 cgcttaagca cacggatgag agacagcctc ctctcctccg tgtgttacta taaaagttat
     1261 ctcccttctc gttcatcgtt ccatatttga gaaacagtat gtcttccaga gttttgaccc
     1321 cggacgtcgt tggtattgac gccctggtac acgatcacca aaccgttctg gcaaaagctg
     1381 aaggcggtgt ggttgccgta tttgctaaca atgccccggc gttttatgcc gtcacgcctg
     1441 cacgcctggc tgaactgctg gcgctggaag aaaagctagc gcgtccggga agcgatgtcg
     1501 ctctggacga tcaactctat caggaaccgc aagccgctcc cgttgctgta cccatgggga
     1561 aattcgccat gtatccggac tggcaacccg atgccgattt tatccgcctg gcggcgctat
     1621 ggggcgtggc gctaagagag ccggtgacca ccgaagaact ggcctcattc attgcctact
     1681 ggcaggcgga aggtaaagtc tttcaccatg tgcagtggca acaaaaactg gcgcgcagcc
     1741 tgcaaatcgg tcgtgccagc aacggcggac tgccgaaacg agatgtgaat acggtcagtg
     1801 aacctgacag ccaaattcca ccaggattca gagggtaacg atgaaaaacg ttggcgacct
     1861 gatgcaacgc ctgcaaaaaa tgatgcctgc ccatatcaaa cctgcattca aaacgggtga
     1921 agagctactg gcctggcaga aagaacaagg ggcgatccgc tccgccgctc tcgaacgtga
     1981 aaatcgggcg atgaaaatgc agcgcacctt taaccgctcc ggtattcgtc cactgcatca
     2041 gaactgctcc tttgagaact atcgcgttga gtgtgaaggg cagatgaatg cgttaagcaa
     2101 agcgcgccag tatgtcgaag agtttgacgg caacatcgcc agcttcatct tttctggtaa
     2161 gccaggaacc ggcaaaaacc atctggcggc ggcaatctgc aacgagctgc tgctacgcgg
     2221 taaatccgta ttgatcatca ccgtggccga tattatgtcg gcgatgaaag ataccttcag
     2281 gaatagcggt accagcgaag aacaactgct taacgatctg agcaacgttg atctgctggt
     2341 gatcgatgag atcggcgtgc agaccgaatc gaaatacgaa aaagtgatca tcaaccagat
     2401 cgtcgatcgc cgctcttctt ccaaacgccc aaccgggatg ctgaccaaca gtaatatgga
     2461 agagatgacc aagttgctgg gcgaacgcgt gatggaccgt atgcgcctgg gtaacagttt
     2521 atgggtgatc ttcaactggg atagctaccg tagccgggta acgggtaaag agtattaatc
     2581 cccgtttctg gcggtatact gcgacacaat tccaccttga gtaatgatga tgaaaactat
     2641 taaacatctt ctgtgctgtg ccattgccgc cagcgcatta atttccaccg gggtgcatgc
     2701 tgcgtcctgg aaagatgcgc tctccagcgc cgccagcgaa cttggcaacc aaaacagcac
     2761 gacacaggaa ggcggttggt cgctcgcgtc attaactaac ttgcttagca gcggaaacca
     2821 agccttaagc gcagataaca tgaacaatgc cgcaggcatt ctgcaatact gcgcgaagca
     2881 aaagctggct tcggtaaccg atgccgaaaa catcaagaac caggtgctgg aaaagctggg
     2941 cctgaacagt gaagagcaaa aagaagacac caactatctg gacggtattc agggtttgct
     3001 gaaaacaaaa gatggtcagc aactcaatct ggataacatc ggaacgactc cgctggcaga
     3061 aaaggtgaaa accaaagcct gcgatctggt gttaaagcag gggctgaact tcatttcctg
     3121 aaccgcgaaa tgttacccgg ctgacgcggc tctctcctgc ccttttccct gctcctggac
     3181 ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa aagtctgttt ataaactaat
     3241 tcctaacaac atcgcatttt tttgacacta gaattgcagc aatatggcga cgccattaca
     3301 ttgtgcttct caaaaagctt aaacgttggc taaacggccc ggtttagtga ggatcgccca
     3361 ttgtcagaac tactctcttt cgcccttttt ctcgcctctg tgctgattta cgcatggaaa
     3421 gcgggacgta acacctggtg gtttgcagcc acgttaacgg tgctggggct atttgtcgtt
     3481 ttaaatatca ccctgtttgc cagcgactat tttactggcg atggtattaa cgacgcggtt
     3541 ctctatacct taaccaacag cctgaccggt gctggcgtca gcaaatacat cctgccgggt
     3601 atcggcattg tgctggggct gacggcggtg ttcggtgcgc tgggctggat cctgcgccgt
     3661 cgtcgtcatc atccgcacca ttttggttac agcctgctgg cgctcttact ggcgctgggt
     3721 tcggtggacg ccagcccggc atttcgtcag ataacggaac tggtgaaatc ccagtcacgc
     3781 gatggcgacc cggactttgc ggcttattat aaagagccgt cgaaaactat ccctgacccg
     3841 aaactcaacc tggtttatat ctacggcgaa agtctcgagc ggacctattt tgataacgaa
     3901 gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa atgaaggcct ggatttcagc
     3961 cacacacagc agctgccagg aacggattac acgattgcgg gcatggtggc ttctcagtgc
     4021 ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg cctctgtctc cagcttcttc
     4081 ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg gttatcagaa ctatttcgtg
     4141 cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc tgaaatcgca cggcttcgac
     4201 cacttatacg gctcagaaga gctgaaaagc gtggtagccg acccgcacta tcgcaacgac
     4261 tggggattct acgacgatac cgttctcgat gaagcgtgga aaaagtttga agagctttcc
     4321 cgctcaggtc agcgattctc actgtttacc ctgacagtcg atacccatca cccggatggt
     4381 tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg acggtaagcc gaatcagtca
     4441 ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt ttatcaacaa aatcaaagcg
     4501 tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg accatttagc gatgaacaac
     4561 acggcgtgga aatacctcaa taagcaggat cgcaataacc tgttttttgt cattcgtggc
     4621 gacaagccgc agcaaaagac gctggcagtg aagcgtaaca cgatggataa cggcgcgacg
     4681 gtgctggaca ttctcggtgg cgataactat ctcggacttg gtcgtagcag tttatccggg
     4741 cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa cattggcgtg gaagccagat
     4801 atcatccgcc tgtggaaatt ccctaaagag atgaaagagt tcaccatcga ccagcagaaa
     4861 aacatgattg ccttctcggg tagccatttc cgtttgccgc tgctgttgcg ggtttcagac
     4921 aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac cgctgcgttt ccagctggcc
     4981 gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt gctacaagat ggcacaactc
     5041 tgggctccgg aactggcact ttccaccgac tggtgtgtct cgcaggggca gcttggcggt
     5101 cagcaaattg ttcagcatgt tgacaaaaca acatggaaga gcaagacggc atttaaagat
     5161 acggtcatcg acatggcgcg ttacaaaggc aatgtcgata cgctgaagat tgttgataac
     5221 gatattcgct acaaagccga cagtttcatc tttaacgtcg ccggtgcgcc agaagaggtg
     5281 aaacagttta gcgggatttc ccgtccggag tcgtggggcc gctggtccaa cgcgcagctg
     5341 ggcgatgaag taaaaatcga gtacaagcat ccgctgccga agaaatttga cctggtgatt
     5401 accgccaaag catacggcaa taacgccagc cgtcctattc cggtacgcgt aggcaatgaa
     5461 gaacaaactc ttgtgctggg caatgaagtg accaccacca cactgcattt cgataaccca
     5521 accgatgccg acacactggt aattgtgccg ccggaacctg tctcaaccaa cgaagggaat
     5581 atcctcggac actcgccgcg taagctcggg atcggcatgg tggaaattaa agtggtagaa
     5641 cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg gtgcgaccta cacgccttct
     5701 ccggcttaca acacattatt aagaaacaaa aaaaacagcg cccaacactt ccccggacgc
     5761 tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccctt aatcaactca
     5821 cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg atagcgatgg
     5881 gttaacatca tgtcggcagt gattttccct tccgccatca gtcgaccgac tttagcgaaa
     5941 tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt cttttttatg gaattccgga
     6001 tcggagaact gcaactcacc tttaaacaga ccgacaaata ccaccgtgcc gccgtgacga
     6061 atcagattca cggtattatt catcgcatgt tgattacctg tcgcgtcgat cactttctgc
     6121 gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa aatcttcggc tgacgggtcc
     6181 agcacgggca actccagacg cgttgccaca tgttcacggc gcgccggact ggtatccgcc
     6241 accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc gattggcccc
     6301 gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac cgcatgagcg
     6361 ctaatagcga aaggttcaat caatgcggcc gcctgcgggt caataccgtc tgcgggcaaa
     6421 atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg cacgccaatg
     6481 actgaaattt tttcacagca gttggtacgc ccgcttttac acgccgggca ttgctgacag
     6541 gcaacataag ggatcacagc aacttgctga ccatttttaa gatcagcaat atttttaccc
     6601 agcccaacaa tctccccaca tatttcatgg cctaaaaccc gtggataact aaaaaatggt
     6661 tgatttccac cctaggcatg aatatcggta ccgcaaatcc cgacagactt aatttttatt
     6721 aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa ttctttcggc
     6781 tgctggcaaa ttaaaacatt catcgtagac atgattcggc tatccatttt ttgttgcctt
     6841 agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt gaaaacacac
     6901 gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat atgagtcgtt
     6961 cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg cggggtcata
     7021 tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc agccgcacta
     7081 cagtgcgtca tattctcagc caattacgcg aatgcggcgt cttaacgcag gttggaaacg
     7141 actatgttat tgcgcgtaaa cctgaccatg atgacggttt cgcctgtact accgcttcaa
     7201 tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gatgataaac cagcgtcagt
     7261 tacgccctag ggaaactttt tctgaactgc aactggctcg ggccgcgggt gtcagcccgg
     7321 tggttgtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa agcgaaaagc
     7381 gcggccagtg gagcatgaag caattcgatc aatcctacgc cgagcagctg tttgagctac
     7441 gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat catgatcccc
     7501 gctggctgca ggcgaaaacc atgctagaac gtcatcggct actgcgtgac aacatcggca
     7561 atagctttcg catgttctcg caacttgaca gagattttca ctccttactg ctttcagctg
     7621 ccgacaatat cttttttgat caatcgcttg agatcatctc agtcatcttt cattttcact
     7681 atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat gaacatatga
     7741 ccattctcag cgctctgatt tgccgcagtg accttgatgc cacccttgcg ctccgtaacc
     7801 atttgaattc agctaaacaa tcaatgattc gatccattaa tgagaacacg cgttatgctc
     7861 attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat caaaagataa
     7921 ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat gtttttgtga
     7981 gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg taatgcacaa
     8041 ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga aaatatcacc
     8101 ctcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt gccaccagat
     8161 ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat gttgttatta
     8221 ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggtagc aaatttaacg
     8281 attcgtgaag aattgggatt aagtgccacc gaaatcggcg ctttgctctc cgtgttttca
     8341 ctcgcttacg gcattgcgca acttccttgc ggcccactat tggatcgtaa aggcccacgc
     8401 ctgatgctgg gactggggat gttcttctgg tcactgttcc aggccatgtc tggcatggtg
     8461 cacagcttta cgcagttcgt gttggtgcgt atcggtatgg ggattggtga agcgccgatg
     8521 aacccatgcg gtgtaaaagt cattaacgac tggttcaaca tcaaagagcg cggacgcccg
     8581 atgggcttct tcaacgcagc ttctaccatt ggcgttgctg taagcccacc gattctggcg
     8641 gcaatgatgc tggtgatggg ctggcgcggg atgtttatta ccattggtgt actggggatt
     8701 tttctcgcca tcggctggta tatgctctat cgcaaccgcg agcacgtaga actgactgcc
     8761 gttgaacaag cttatctcaa tgcaggtagc gtcaatgccc gccgagatcc gctcaatttt
     8821 gccgaatggc gcagcctgtt ccgtaaccgc acaatgtggg gaatgatgct cggattcagt
     8881 ggcatcaact acactgcgtg gctgtatctg gcctggcttc ctggttacct gcaaacagcc
     8941 tataacctgg atttaaaaag cacagggttg atggcggcta tccctttcct gtttggggct
     9001 gccgggatgc tggtcaacgg ttacgttact gactggctgg tcaaaggggg aatggctccg
     9061 attaaaagcc gtaagatctg cattattacc gggatgttct gttctgccgc ctttacgctg
     9121 atagtaccac aagcgacaac atccatgatg gcggttctgc tgattggcat ggcactgttc
     9181 tgtattcact ttgccggaac atcctgctgg ggcttgatcc acgtcgcagt tgcttctcgc
     9241 atgactgcgt cggtgggcag tatccagaac tttgccagct tcatctgcgc ctctttcgcg
     9301 ccgatcatta ctggttttat tgttgataca acccactcat tccgtctggc actaatcatt
     9361 tgcggttgcg tcaccgcagc gggagcactg gcgtacatct tcctggttcg ccagccgatc
     9421 aacgacccgc gtaaagatta aactccctat gcccgataac attttgctta tcgggcattt
     9481 ttatggcgat taaaacgttt cccagttctc cccgctatct gccactgcca ttttacgcgg
     9541 cgtagctggc gtcacggttt ttaccacagc cgatgtttca cgctgctgtt gctgctgttg
     9601 ctgaatccgg aacactgcca cagcttcggt caggcgactg gcctgctctt ccagcgcggc
     9661 ggcggcagcg gcagattctt ccaccagcgc ggcgttctgt tgagttaccc ggtccatctc
     9721 agcaaccgct aagccaacct gatcgatacc acggctctgc tcatcagaag cagaagatat
     9781 ttcgcccata atgtccgtca cgcgggtcac agcactgaca atctccgcca ttgtttcccc
     9841 ggcgctttcg accagcgtag agccaacatc cactttcccc accgagtctt caatcaggct
     9901 tttaatttca cgaaccgcct gggcgctacg ctgggccaga ttacgcactt ctcccgcaac
     9961 caccgcaaaa ccacgtcctt gctcacccgc acgcgctgct tcaaccgccg cgttcaaagc
    10021 cagaatattg gtctggaagg taatgccgtc aattacgctg ataatatcgg cgattttctg
    10081 cgaactggtg gagatatcgc gcatagtctg caccacgtta tctaccactt tgccgccgcg
    10141 ttgcgccgtt tcagaagcac ttaatgccag atggctggcc tggcgcgcat tctcggcgtt
    10201 ctgtttcacc gttgcggtca gttgctccat gctggctgcc gtctcttcca gcgaagcggc
    10261 ctgttgctcg gtgcgcgaag agagatcatt attgccggta gcgatttcgc tggcaccgct
    10321 atagatggca ttggccccgt tgcgcacatc accgacggta cgcatcagct ctccctgcat
    10381 atggcgcaaa ctctctgcca gttgccccat ctcattagag ccatccacct caatcggttt
    10441 caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca ttggcgctac
    10501 cagcgaggct ttaataccga accagacggc gaagatgacc gccagtacga cgatcatcac
    10561 gcccaccaga atccacatcg cctggctgta ggaggcattg ttatcgctga cggcgatatc
    10621 atagagccga tcgttttgct ccatgtaagc cacatactgc ttctcgaaac cgtcctgata
    10681 tccctgggtc ggctgatcaa agaactcgtt gattttgcct gcacctaaca gttggatcag
    10741 ctccgccagc gcattgtgat aaatatcgta attacgtttg atctctgccg ctgcggcggt
    10801 gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt ccgcctgttt
    10861 cagcgaaata ctggcactct gcatcagctc agcaacggtt gaaccgctac caatattatt
    10921 ctgatccatc atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc
    10981 gacccagctg ccattcagcg tgggttgctg ctggcgaatg gtttgtaaaa cagtgaaatt
    11041 ttctttgtca ttctttaagg cattaaaaaa cagaccgcct gatgtcagtt gtaaaaggcc
    11101 aaaaacggcc aaaaccagca gtaagctggt cacgatcttg atacgtttta acatggtttc
    11161 tctttcctgt ggaccgatgc aagattttcg gcctggaaag caaaaacttt atgaaaaggt
    11221 gtaacccgcc atccatggaa aaaccaggcg ctggttatca gatactaaaa agttattcat
    11281 tatcgcccgg gatgtcttct tgtcgggaat tgcccagagc aataaattcc tctaacagat
    11341 gggttaactg ttgcattttc tcggcagtaa actgagcttc aatctgccga taggcttctt
    11401 caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc tttggtcagc gagatataga
    11461 gcttccgctg atcattgatc ggctttaatc gcaacactaa accgtcgcgt tccatccgcg
    11521 tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg atacgccaga tcgtgaaaat
    11581 ccattgatgg gctttccgcc agaatacgca cgatgcgcca ctgttgctca gtcaaattat
    11641 gccgtttaac gattgggcga aaataactca tcgccgcttc tcgcgcctgg agcaacgcaa
    11701 tggttagtga gtcgtgcatt atctttcccc tgaaaaattc aaaatcatta atatagaaac
    11761 aattaatcat cacgttaccg ggactgagct gcccccgcaa ttcttcattt tatcaaaatt
    11821 gatgatattt tattaactta ctgatattta atattttatt atctttaaat gtaaatagtt
    11881 tgttaattag atcacattta tatcacttac ttttgccaaa ctacgcaatc gggaataaaa
    11941 gtaatcatta acatattaat gattaagatg atcgaatccg aggagtggta atgaaaggca
    12001 ctatcttcgc cgtagcgttg aaccatcgca gccaacttga tgcctggcag gaagcgttcc
    12061 agcaatctcc ctacaaagcc ccgcctaaaa ctgcggtctg gtttattaaa ccgcgcaata
    12121 cggtgattgg ttgcggtgaa ccgattccct ttccacaggg tgaaaaagta ctgagcggtg
    12181 cgactgttgc gctgattgtg gggaaaacgg cgacgaaagt acgtgaagaa gatgcggcag
    12241 agtacatcgc cggatatgcg ctggataacg acgtcagcct gccggaagag agcttttacc
    12301 gtccggcaat caaagcaaaa tgtcgtgatg gattctgccc cattggcgaa accgtggctc
    12361 tcagcaatgt cgataatctg actatctata ccgagatcaa cgggcgtcct gccgatcact
    12421 ggaataccgc cgatttacaa cgtaacgccg cacagctgct gagcgccctg agcgaatttg
    12481 ccacgctgaa tccaggcgat gctattctgc tcggcacgcc acaggcgcgc gtggaaatac
    12541 agccaagcga tcgcgtgcgt gttctcgcag aaggtttccc gccgctggaa aatccggtag
    12601 tggacgaacg tgaagtgacc acgcgcaaga gcttcccaac gctgccacac ccgcacggta
    12661 cgctgtttgc cctcggcctg aactacgccg accacgccag cgaactggaa tttaagccac
    12721 cggaagagcc gctggtgttc ctgaaagcgc cgaataccct cactggcgac aaccagacct
    12781 ccgtgcgccc gaacaatatt gaatacatgc actacgaagc ggagctggtg gtagttattg
    12841 gcaagcaggc gcgtaacgtc agcgaagccg atgccatgga ttatgtcgcg ggctacaccg
    12901 tgtgtaacga ctacgccatt cgcgactatc tggaaaacta ctaccgccct aacctgcggg
    12961 tcaaaagccg cgacggactg acgccgatgc tttcaaccat cgtgccgaaa gaggcgatcc
    13021 cggacccgca taatctgacc cttcgcacct tcgtcaacgg cgagttacgc cagcaaggca
    13081 ccaccgccga tctgatcttc agcgtgccct tcctgatcgc ctacttaagc gaatttatga
    13141 ccctgaatcc gggcgacatg atcgccaccg gcacaccaaa aggcttatct gacgtggtgc
    13201 ctggcgatga agtagtggtg gaagtagaag gcgtgggccg cctggtgaac cgaattgtga
    13261 gtgaggaaac agcgaaatga aaaaagtaaa tcattggatc aacggcaaaa atgttgcagg
    13321 taacgactac ttccagacta ccaatccggc aacgggtgaa gtgctggcgg atgtggcctc
    13381 tggcggtgaa gcggagatca atcaggcggt agcggcagcg aaagaggcgt tcccgaaatg
    13441 ggctgatctg ccgatgaaag agcgtgcgcg cctgatgcgc cgtctgggcg atctgatcga
    13501 ccagaacgtg cctgagatcg ccgcgatgga aaccgcggac acgggcctgc cgatccatca
    13561 gaccaaaaat gtgttgatcc cacgcgcttc tcacaacttt gaatttttcg cggaagtctg
    13621 ccagcagatg aacggcaaga cctatccggt tgacgacaag atgctcaact acacgctggt
    13681 gcaaccggta ggcgtttgtg cgctggcgtc gccgtggaac gtgccgttta tgaccgccac
    13741 ctggaaggtc gcgccgtgtc tggcgctggg caataccgcg gtgctgaaga tgtcggaact
    13801 ctccccgctg accgccgacc gcctgggtga gctggcgctg gaagctggta ttccggcggg
    13861 cgtgctaaac gtggtacagg gctacggcgc aaccgcaggc gatgcgctgg tccgtcatca
    13921 tgacgtgcgt gccgtgtcgt tcaccggcgg taccgccacc gggcgcaaca tcatgaaaaa
    13981 cgccgggctg aaaaaatact ccatggagct gggcggtaaa tcaccggtgc tgatttttga
    14041 agatgccgat attgagcgtg cgctggacgc cgccctgttc accatcttct caatcaacgg
    14101 cgaacgctgc accgccggtt cgcgcatctt tattcagcag agcatctatc cagaattcgt
    14161 taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc gatccgaacg atccgaatac
    14221 ccaggttagg gcgcttatca gtcagcaaca ctgggaaaaa gtctccggct atatccgtct
    14281 cggcattgaa gaaggcgcaa ccctgttggc gggcggcccg gataaaccgt ccgacctgcc
    14341 tgcgcacctg aaaggcggca acttcctgcg cccaacggtg ctggcggacg tagataaccg
    14401 tatgcgcgtt gcccaggaag agattttcgg gccggtcgcc tgcctgctgc cgtttaaaga
    14461 cgaagccgaa ggcttacgcc tggcaaacga cgtggagtac ggcctcgcgt cgtacatctg
    14521 gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc attgaagcag gcatggtgtt
    14581 cgtcaacacc cagaacgtgc gcgacctgcg ccagccattt ggcggcgtaa aagcctccgg
    14641 caccgggcgt gaaggcggtg agtacagctt cgaagtgttc gcggaaatga agaacgtctg
    14701 catttccatg ggcgaccatc caattccgaa atggggagtc tgatatgggt aagttagcgt
    14761 tagccgccaa gatcacgcac gtaccgtcga tgtatctctc tgaactacca ggaaaaaacc
    14821 acggttgtcg ccagggtgcg atcgacgggc ataaagagat cagcaagcgt tgccgggaaa
    14881 tgggcgtcga taccattatc gttttcgata ctcactggct ggtcaacagt gcttatcaca
    14941 tcaactgtgc agaccatttt gaaggcgtct acaccagcaa cgagctgccg cattttattc
    15001 gcgacatgac ctacaactac gagggcaacc cggagttggg gcagctaatt gccgaggaag
    15061 ccttaaagct cggcgtgcgg gcaaaagcgc acaatattcc cagcctgaaa ctggaatacg
    15121 gcacgctggt accgatgcgc tacatgaatg aagacaagca cttcaaagtg gtctccattt
    15181 cggctttctg cacggttcac gattttgccg acagccgcaa gctgggcgaa gcgattctga
    15241 aagcgatcga acagtacgac ggcaccgtgg cggtccttgc cagcggttcg ttatcgcacc
    15301 gctttattga cgatcagcgt gcggaagaag ggatgaacag ctacacccgc gagttcgacc
    15361 gccagatgga cgagcgtgtg gtgaagctgt ggcgcgaagg ccagttcaaa gagttttgca
    15421 atatgctgcc ggagtacgcc gactactgct acggcgaagg caatatgcac gacacggtga
    15481 tgctgctggg gatgctcggc tgggataaat acgacggcaa ggtggagttt attaccgagc
    15541 tgttcccaag ctctggcacc ggtcaggtta acgctgtttt cccgctgccc gcgtaaggac
    15601 gttttatgcc gcactttatc gtggaatgta ctgaaaatat tcgtgaagaa gcccgcttac
    15661 ctgagctgtt tgccagtgtt aacactgcac tggcggcaac ggggattttt ccgcttgggg
    15721 gaatacgtag ccgggcgcac tggatcgata cctggcaaat ggctgatggc aagcacgatt
    15781 acgcctttgt gcatatgacg ctaaaaatcg gcagcggacg cagtctggaa agccgtcagg
    15841 aagtgggcga aatgctgttt gatctgatta aaacgcactt cgcctcactg atggagagcc
    15901 gttacctagc gctttcattt gagattgcag agctgcaccc gacgttgaat ttcaaacaga
    15961 acaacgtaca cgcgttgttt aaataaattt gcaccagctt ccaggccgga tgataaggcg
    16021 ctgccgcatc cggcaatgcc acaggatatc gctatgttcg ataaacacac ccacaccctg
    16081 atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac agatccgcgc gatctcgctg
    16141 gattacccgg agatcaccat cgaagacgcc tacgccgtgc agcgcgaatg ggttcgcctg
    16201 aaaatcgccg aaagtcgcac gctgaaaggc cacaaaatcg gtctgacctc gaaagcgatg
    16261 caggcaagct cgcagatcag cgaaccggat tacggtgcac tgctggacga catgttcttc
    16321 cacgatggca gcgatatccc gaccgatcgc tttatcgtgc cgcgcattga agtggagctg
    16381 gcttttgtgc tggcaaaacc gctgcgtggg ccaaactgca cgctgttcga cgtttacaac
    16441 gccacggact atgtgatccc ggcgctggag ctgatcgacg ctcgctgcca caacatcgat
    16501 ccggaaaccc agcgcccgcg caaagtattc gacaccattt ccgataacgc cgccaacgcc
    16561 ggagtgatcc tcggaggtcg tccgatcaaa ccagacgaac tggatctgcg ttggatctcc
    16621 gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg tcgccgctgg tgtgctgaat
    16681 catccggcaa acggcgtggc ctggctggcg aacaaactcg caccgtatga cgtacaactg
    16741 gaaaccgggc aaatcattct cggtggttcg ttcaccctcc cggttccggc gcgtaagggc
    16801 gacaccttcc acgtcgatta cggcaacatg ggctccatta gctgccgctt tgtttaagga
    16861 gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca ggccgtccgc agattggatt
    16921 atggctgggg ctgagcagca gctacagcgc ggagttactg gccggaccag gattcgactg
    16981 gttattgatc gacggtgagc acgcgccgaa taacgtgcaa accgtgctca cccaactaca
    17041 ggcgattgcg ccctacccca gccagccagt ggtacgccca tcgtggaacg atccggtgca
    17101 aatcaaacaa ctgctggacg taggcacaca aaccttactg gtgccgatgg tacaaaacgc
    17161 cgacgaagcc cgtaaagcgg tacgcgccac ccgttatccc cccgccggta ttcgcggtgt
    17221 gggcagtgcg ctggctcgcg cctcgcgctg gaatcgcatt cctgattacc tgcaaaaagc
    17281 caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt gaggcaatga agaacttacc
    17341 gcagattctg gacgtggaag gggtcgacgg cgtgtttatc ggcccggcgg atctgagcgc
    17401 cgatatgggt tatgccggta atccgcagca cccggaagta caggccgcca ttgagcaggc
    17461 gatcgtgcag atccgcgaag cgggcaaagc gccggggatc ctgatcgcca atgagcaact
    17521 ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc gtcggcgttg acaccaccct
    17581 gctcgcccgc gccgccgaag cgctggcagc acggtttggt aatagttctt ctatttcgat
    17641 ggcgaagcaa aacaacaact ctgtctacta aacgtacgat gtggagcgca tcatgacgac
    17701 ctctaccctg caatataaag ataataaaac tgttgaaatt gaacatcgtg tcattaataa
    17761 attatttcgt cgcctaatca tttttctttt tattctgttt gttttttcgt ttcttgatcg
    17821 catcaatata ggatttgccg gactgacaat gggcaaggat cttggcctga cgtccacaat
    17881 gtttggcctc gccgcaacac tcttttatgt cacgtacgtc atgtgcggga tccccagtaa
    17941 tatgatgtta gcgaaaattg gcgctcgtcg atggatcgct gggatcatgg tggtctgggg
    18001 aattgcttcc acctgcacaa tgtttgcaac aagccccgaa acactctatc tactgcgtat
    18061 gctggttggt attgctgagg cgggtttttt acctggtatt ttggtttatc tgacctggtg
    18121 gttcccggca tatcatcggg ccagagccaa tgccttattt atgatagcga tgccagtcac
    18181 tatgatgctg ggattgatcc tttcaggcta cattctggcc ttggatggac tgtggaatct
    18241 aaaaggctgg caatggctct ttttgctgga aggtctgcca tcggtcattc ttggtgtcgt
    18301 cacctggttt tatctcaacg acacaccaga ccaggcgacc tggcttgatg aaaacgaaaa
    18361 acaggcttta aaaacgatga ttctccgtga acaagagagt gcatttgccc atgcatctgt
    18421 tccacgctca accttacgtg aagtcctgac cccagccatc atgctatata cacttgctta
    18481 tttttgcccg actaatactc tgagtgcgat taatatctgg acgccgcaaa tcctacagag
    18541 tttcaatact ggcagtagca acatcatgat tggtttgctg gcggccatcc cccaattctg
    18601 cacaattttt ggcatgatct ggtggagtcg tcgttccgac cgtcttaaag aacgcaaaaa
    18661 gcatacgatt cttccctacc tgtttgccgc tgccggatgg ttactcgctt ctgcgacaga
    18721 tcacagcctc attcaactga gcagcatcat tatggcctca atgggatcat ttaccgcgat
    18781 gactatcttt tggacaacac cggatcaagt tataagcttg caatcgcgtg ccgttgcgat
    18841 ggctgtcatc aatgctgtcg gcaatgtagg ttcagcagtc agtccattac ttatcggtat
    18901 tttgaaagat gcaaccggca gttttagctc aggtttgtgg tttgtagcag gattgctggt
    18961 actgggcgcg ttagtcatga cccgaatccc tatggggagt cgggaacact ctctttcatc
    19021 tgaacttatc gcaccaaaac cgcaataagg agtcatgatg tccagtgaag ctatctttga
    19081 acatggttat gaaaatattg atttggtccg ggaatatgat gcccgctacg ctggtgaaga
    19141 tgtgcattat gaaacatttg cccggttagc gaatttcttt ggacgcgata tgcgccctca
    19201 ctggcacgat agatattttc aactccatta tctggtcact ggacgcatta ctttacaact
    19261 ggatgagcac ttctattctg tgcgcgcacc gctttttgta ctaacaccac catcggtgcc
    19321 acatactttc tttactgatc ctgatagcga tgggcatgtt ctgaccgtac gtcaggagtt
    19381 aatttggccc ctggtagaaa agctctggcc tggtagtgga gatgccgcaa tgctacaagg
    19441 tatctgtctg tcactggagg gaatggaaca tacactggcg gcattaaatc attattggcc
    19501 gataatatcc ctggagtttc aacaaaataa gcaaggacgg gaaattctat tgcaatcact
    19561 ggcgcaaagt atttttacaa tcctactgcg tgaagcacca ccaaatgata tttcaacatg
    19621 cagcgtgcgc ggcgagatgt gtttattcca gaagtttaat cggctgattg acgaaaatta
    19681 tcgccaacat ttaatggtcc ctgaatatgc aacaatgctc ggtatgagtg aatcacggct
    19741 taccgagctg tgccgacgtt ttgccaatca atcgccgaag agattgattt tcgaacgagt
    19801 gagccgggaa gccagacggc ttctgctcta tagcgagcaa agcatcaacc agatagccct
    19861 tgatcttggc tacaaagatc ccgcctattt cgcccgtttt ttcaaccgaa tgatgggttg
    19921 ctcaccaaca caattccgtg gtcgctgacc atttatcaac ctgagagact cgacatgaaa
    19981 atatctttac gctcactatt tgtggcgtgc ctgattgcgc cagtggcatt cacctacgcc
    20041 tccgaaccgc aataaatgaa aattgtacta cttggaacga aaggtggccc ttctttatta
    20101 aaagctgacc gtttaccgat ggcaacggca cttattattg gcaataaaac ctggttaatt
    20161 gatgctggtt acggggccag cttacaactg gtaaaaaata atattccact gcgccagatt
    20221 gataatattt tactcaccca tttacacagt gatcatattc tggactatcc atcagtctta
    20281 atgaatgcat gggctagcgg tctgcaggac aaaactattc atgtattcgg tccgccaggc
    20341 acccgagcca tgaccgctgc cagttggcag gtcttcgatc gggacatcac actgcgtatg
    20401 gaagaagaag gaaaacccga tcctcgcaaa ctcgttacag ctcaagatgt tggtgaaggc
    20461 gtagtttata aagatgatac cgtgaccgtg actgcattaa aggtccctca ttctcctttc
    20521 cctgacggtg aggcttttgc ttatcgcttc gacacacaag gcaagcgaat tgtcttctca
    20581 ggagatacct cgtactttcc cccactggca actttctcag aaggcgctga tatacttgtg
    20641 catgaggccg tacatgagcc ctccgtcgct aagctggcag acagtattgg caatggtaaa
    20701 acactggcaa aagccattgc ttctcaccat acgactattg aggatgtagg caaaattgcc
    20761 aatgaagcaa aagtgaaaaa actcatcctt agccacctgg taccaatcac cgttccggat
    20821 gaaacatgga agcaagaagc acagaaaact attcaggtcc tatcgtcgtt ggtcatgaaa
    20881 atatgacgat taccattcca ttcgccccat aaaaaattcc gtccccgtta gcccttgtta
    20941 gcggggaata ttatgatttg ccttactgta agttgatcac gatcgcagtt tactatttaa
    21001 ctccaccttg ctgtcgaaaa gtaccaacgt tcgcctgaaa cgtctcttcc cgacaaccgc
    21061 gttcaacgct tcaattaaat aacaaaaaca acacacaaaa ttaacaatcg tattccgatt
    21121 aatactgtag aggtcgacat gaaaccagaa gatttccgcg ccagtaccca acgtcctttc
    21181 accggggaag agtatctgaa aagcctgcag gatggtcgcg agatctatat ctatggcgag
    21241 cgagtgaaag acgtcaccac tcatccggca tttcgtaatg cggcagcgtc tgttgcccaa
    21301 ctgtacgacg cgctgcacaa accggagatg caggactctc tgtgttggaa caccgacacc
    21361 ggcagcggcg gctataccca taaattcttc cgcgtggcga aaagtgccga cgacctgcgc
    21421 cagcaacgcg acgccatcgc tgaatggtca cgcctgagct atggctggat gggccgtacc
    21481 ccagactaca aagccgcttt cggttgcgca ctgggcgcga atccgggctt ttacggtcag
    21541 ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg aaactggcct ctactttaac
    21601 cacgcgattg ttaacccacc gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca
    21661 tcaagctgga aaaagagact gacgccggga ttatcgtcag cggtgcgaaa gtggttgcca
    21721 ccaactcggc gctgactcac tacaacatga ttggcttcgg ctcggcacaa gtgatgggcg
    21781 aaaacccgga cttcgcactg atgttcgttg cgccaatgga tgccgatggc gtgaaattaa
    21841 tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg ctcgccatac gactacccgc
    21901 tctccagccg cttcgatgag aacgatgcga ttctggtgat ggataacgta ctgatcccat
    21961 gggaaaacgt gctgatctac cgcgattttg atcgctgccg tcgctggacg atggaaggcg
    22021 gtttcgcccg tatgtatccg ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca
    22081 ttacggcact gctgaaaaaa tcactcgaat gtaccggcac cctggagttc cgtggtgtgc
    22141 aggccgatct cggtgaagtg gtggcgtggc gcaacacctt ctgggcattg agtgactcga
    22201 tgtgttcaga agcgacgccg tgggtcaacg gggcttattt accggatcat gccgcactgc
    22261 aaacctatcg cgtactggca ccaatggcct acgcgaagat caaaaacatt atagaacgca
    22321 acgttaccag tggcctgatc tacctccctt ccagtgcccg tgacctgaac aatccgcaga
    22381 tcgaccagta tctggcgaag tatgtgcgcg gttcgaacgg tatggatcac gtccagcgca
    22441 tcaagatcct caaactgatg tgggacgcca ttggcagcga atttggtggt cgtcacgagc
    22501 tgtatgaaat caactactcc ggtagccagg atgagattcg cctgcaatgt ctgcgccagg
    22561 cacaaagctc cggcaatatg gacaagatga tggcgatggt tgatcgctgc ctgtcggaat
    22621 acgaccagaa cggctggact gtgccgcacc tgcacaacaa cgacgatatc aacatgctgg
    22681 ataagctgct gaaataacgc agcaggaggt taagatgcaa ttagatgaac aacgcctgcg
    22741 ctttcatgac gcgatggcca gcctgtcggc agcggtaaat attatcacca ccgagggcga
    22801 cgccggacaa tgcgggatta cggcaacggc tgtctgctcg gtcacggata caccaccgtc
    22861 gctgatggtg tgcattaacg ccaacagtgc gatgaacccg gtttttcagg gcaacggcaa
    22921 gttgtgcgtc aacgtcctca accatgagca ggaactgatg gcgcgccact tcgcgggcat
    22981 gacaggcatg gcgatggaag agcgttttag cctctcatgc tggcaaaaag gtccgctggc
    23041 gcagccggtg ctaaaaggtt cgctggccag tcttgaagga gagatccgcg atgtgcaggc
    23101 gattggcaca catctggtgt atctggtgga gattaaaaac atcatcctca gtgcagaagg
    23161 tcacggactt atttacttta aacgccgttt ccatccggtg atgctggaaa tggaagctgc
    23221 gatttaagct ccagcccctt cacgcattcg tcgaaggggc atttttattg tgatctcaat
    23281 cacatatcta cccgatgaat aattcttgcc caaccgctca ccgccattcc tgaccgctta
    23341 catccctaaa ataaccactc agttatttac cttactttac gcgcacgtat ctctggcaac
    23401 atcactgcag gatagcggtc aatttacctc ctcaaacgca acgcaaacct agaacggctt
    23461 cggcctatga ttaacaaatt actctatgga ttttggtttg caggaaggca gcaagtgagt
    23521 gaatccccgg gagcttacaa aagtaagtgt cagaggtgaa cgaacgcagc tgccgcacct
    23581 gtaagccaaa agacgacgag taaaatgcca ggttttacta tggatacgaa aaaactattc
    23641 aagcacatac cctgggtgat tctcggaatc atcggtgcat tctgcctcgc ggtagttgca
    23701 ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat
    23761 ctggtggcgt atcgctacta cagtctgtac atcgcccaga aggtgatgaa actcgacccc
    23821 acgcgcgcga cgcctgcggt tattaacaac gacggtctga actacgttcc gaccaaccgt
    23881 tacgtgttgt ttggtcacca cttcgccgct atcgccggtg ctggtccgct ggtgggaccg
    23941 gttctcgccg cgcagatggg ctacctgcct ggcacgctgt ggctgctggc gggggtagtg
    24001 ctggccggtg cggtacagga ctttatggtg ctgtttatct cctctcgccg taacggcgca
    24061 tctctcggtg agatgatcaa agaagagatg gggccagtac cggggactat cgcgctgttt
    24121 ggctgtttct taatcatgat catcatcctc gccgttctgg cgctgattgt ggttaaagcc
    24181 ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt
    24241 atgggtatct acatgcgctt tatccgtccg gggcgtgtgg gtgaagtctc tgttattggt
    24301 atcgtgctgc tggttgcctc tatctacttc ggtggcgtga ttgctcacga tccgtactgg
    24361 ggtccggcac tgacctttaa agacaccacc attaccttcg cgctgattgg ctatgcgttt
    24421 gtttccgcac tgctgccggt gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc
    24481 ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg tggtgctgaa cccggaactg
    24541 aaaatgcctg cgatgaccca gtacattgac ggtactggcc cgctgtggaa aggcgctctg
    24601 ttcccgttcc tgttcatcac catcgcctgt ggtgcggtat ctggcttcca cgcgctgatc
    24661 tcttccggta cgacgccgaa actgctggct aacgaaaccg acgcgcgttt cattggctac
    24721 ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc tggttgcagc gtccatcatc
    24781 gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg gccttggcat caccatgcct
    24841 aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt
    24901 accgcacacg cggcagcgac cgtcagctcc tggggcttcg tgatttcgcc agagcagatc
    24961 ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga accgtgcagg tggtgcgccg
    25021 acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc
    25081 ttctggtatc acttcggtat tctgttcgaa gccctgttca tcctgaccgc gctggatgcg
    25141 ggtacccgtt ctggccgctt tatgctgcaa gacctgctgg gtaacttcat cccgttcctg
    25201 aaaaaaaccg actctctggt tgccggtatc atcggtactg cgggctgtgt gggtctgtgg
    25261 ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg
    25321 ctgttcggta tctccaacca gatgctggca gccgtagcgc tggtactggg caccgttgtg
    25381 ctgattaaga tgaagcgcac ccaatacatc tgggtaactg ttgttccggc tgtatggctg
    25441 cttatctgca ccacctgggc gctgggtctg aaactgttca gcaccaaccc gcagatggaa
    25501 ggcttcttct acatggcaag ccagtacaaa gagaagattg ctaacggtac tgacctgacg
    25561 gcgcagcaga ttgccaacat gaaccacatc gttgtgaaca actacaccaa cgcaggcctg
    25621 agtattctgt tcctgattgt ggtgtacagc atcatcttct acggtttcaa aacctggctt
    25681 gcggtgcgta acagcgacaa acgtactgac aaagaaacac cgtacgttcc aatcccggaa
    25741 ggcggcgtga agatctcttc gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt
    25801 gttcttatca gagtgaacta tgtttggtaa cttaggacag gcaaaaaaat atctcggcca
    25861 ggcggcgaag atgttgattg gtattccaga ctacgacaac tacgtcgagc atatgaagac
    25921 caaccatccc gacaagccgt acatgagcta tgaagaattc ttccgcgaac gccagaacgc
    25981 gcgctacggc ggcgatggta aaggcggtat gcgctgttgt taatggagag agcatgaacc
    26041 cgattgcagt taccctactc accggttttt taggcgcggg caaaactacc ctgctgcgcc
    26101 atattcttaa cgaacaacac ggctacaaga ttgccgtgat tgaaaacgaa ttcggcgaag
    26161 tctccgttga tgaccaactg attggcgacc gcgccacgca gatcaaaacg ctgaccaacg
    26221 gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc actactcgac ctgctggata
    26281 atctcgacaa gggcaatatt cagttcgatc gtctggtgat tgaatgcacc ggcatggccg
    26341 atcccggccc gattattcag acctttttct cccatgagat tttatgccag ctctacctgc
    26401 tggacggcgt gattgcgctg gtggatgcgg tacatgccga tgagcagatg aaccagttca
    26461 ccatcgccca gtcccaggtg ggctacgccg accgtattct gctgactaaa accgacgtcg
    26521 caggcgaagc agagaaactg cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct
    26581 acaccgtcac ccacggcgac atcgacctgg gcctgctgtt caacaccaac ggttttatgc
    26641 tggaagagaa cgtcgtcagc accaaaccgc gtttccactt tatcgcggat aaacaaaacg
    26701 atatttcgtc aattgtggtg gaactggatt acccggtaga tatcagcgaa gtttcccgcg
    26761 tgatggaaaa cctgctgctg gaatcggcgg ataaactgct gcgttacaaa gggatgctgt
    26821 ggattgacgg cgaacctaac cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg
    26881 actgggacag gccgtggggc gatgaaaaac cgcatagcac aatggtgttt atcgggattc
    26941 agttgccgga ggaagagatt cgggcggcgt ttgcgggatt gaggaagtaa gttcgggaaa
    27001 gatcaacttt ttgagagttc caccgcaaca ttattacgag tggaactctt tagatatgtc
    27061 agaccaacca aagcttcttc aattgcgttt tcattttttc atgtctcatt ttcagttcag
    27121 ccaatcccca ttctgaagaa ttacaaacat cttttgttaa ataaaaagag cttttactat
    27181 taagtttctc ataaatagat ttctttttga taaaatcata gtgttgtgtt tcagaatttt
    27241 tggaaccact tatgagtgtt aaattaccta ttttatgcag ccagtagaca tgctcttgag
    27301 gttggaatct attaatccag tattcattaa ctaatgcttg aggaagaata tgctcaattg
    27361 tgattctccc cgtataggtt t
//
LOCUS       DAJTYH010000055        27294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTYH010000055 DAJTYH010000000
VERSION     DAJTYH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO609_002251"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO609_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6633652.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO609_002252"
     CDS             complement(1614..1937)
                     /locus_tag="OO609_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6633653.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO609_002253"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO609_002253"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6633654.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO609_002254"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO609_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6633655.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO609_002255"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO609_002255"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6633656.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO609_002256"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO609_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6633657.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO609_002257"
     CDS             7537..8574
                     /locus_tag="OO609_002257"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6633658.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO609_002258"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO609_002258"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6633659.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO609_002259"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO609_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6633660.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO609_002260"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO609_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6633661.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO609_002261"
     CDS             10745..11626
                     /locus_tag="OO609_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6633662.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO609_002262"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO609_002262"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6633663.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO609_002263"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO609_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6633664.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO609_002264"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO609_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO609_002265"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO609_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6633665.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO609_002266"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO609_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6633666.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO609_002267"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO609_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6633667.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO609_002268"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO609_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6633668.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO609_002269"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO609_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6633669.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO609_002270"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO609_002270"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6633670.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO609_002271"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO609_002271"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6633671.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO609_002272"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO609_002272"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6633672.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO609_002273"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO609_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6633673.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO609_002274"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO609_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6633674.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO609_002275"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO609_002275"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6633675.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO609_002276"
     CDS             24893..25273
                     /locus_tag="OO609_002276"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6633676.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO609_002277"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO609_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6633677.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO609_002278"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO609_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6633678.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27294
                     /locus_tag="OO609_002279"
                     /pseudo
     CDS             26442..27294
                     /locus_tag="OO609_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6723 a         6800 c         7156 g         6615 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctgg
//
LOCUS       DAJTYH010000056        26106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTYH010000056 DAJTYH010000000
VERSION     DAJTYH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            129..422
                     /locus_tag="OO609_002280"
     CDS             129..422
                     /locus_tag="OO609_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6633679.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            494..1153
                     /locus_tag="OO609_002281"
     CDS             494..1153
                     /locus_tag="OO609_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6633680.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1245..1691
                     /locus_tag="OO609_002282"
     CDS             1245..1691
                     /locus_tag="OO609_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6633681.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1898..2798)
                     /gene="hlyE"
                     /locus_tag="OO609_002283"
                     /pseudo
     CDS             complement(1898..2798)
                     /gene="hlyE"
                     /locus_tag="OO609_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3171..3590
                     /locus_tag="OO609_002284"
     CDS             3171..3590
                     /locus_tag="OO609_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6633682.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3590..4858
                     /gene="umuC"
                     /locus_tag="OO609_002285"
     CDS             3590..4858
                     /gene="umuC"
                     /locus_tag="OO609_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6633683.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(4904..5434)
                     /gene="dsbB"
                     /locus_tag="OO609_002286"
     CDS             complement(4904..5434)
                     /gene="dsbB"
                     /locus_tag="OO609_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6633684.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5580..7121)
                     /gene="nhaB"
                     /locus_tag="OO609_002287"
     CDS             complement(5580..7121)
                     /gene="nhaB"
                     /locus_tag="OO609_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6633685.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7342..8061
                     /gene="fadR"
                     /locus_tag="OO609_002288"
     CDS             7342..8061
                     /gene="fadR"
                     /locus_tag="OO609_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6633686.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8113..9645)
                     /locus_tag="OO609_002289"
     CDS             complement(8113..9645)
                     /locus_tag="OO609_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6633687.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            9975..11273
                     /gene="dadA"
                     /locus_tag="OO609_002290"
     CDS             9975..11273
                     /gene="dadA"
                     /locus_tag="OO609_002290"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6633688.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11283..12353
                     /gene="dadX"
                     /locus_tag="OO609_002291"
     CDS             11283..12353
                     /gene="dadX"
                     /locus_tag="OO609_002291"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6633689.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12521..13935)
                     /locus_tag="OO609_002292"
                     /pseudo
     CDS             complement(12521..13935)
                     /locus_tag="OO609_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14546..16282)
                     /locus_tag="OO609_002293"
     CDS             complement(14546..16282)
                     /locus_tag="OO609_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6633690.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16377..17291)
                     /gene="ldcA"
                     /locus_tag="OO609_002294"
     CDS             complement(16377..17291)
                     /gene="ldcA"
                     /locus_tag="OO609_002294"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6633691.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17391..18002
                     /gene="emtA"
                     /locus_tag="OO609_002295"
     CDS             17391..18002
                     /gene="emtA"
                     /locus_tag="OO609_002295"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6633692.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18004..18738)
                     /locus_tag="OO609_002296"
     CDS             complement(18004..18738)
                     /locus_tag="OO609_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6633693.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            18939..19193
                     /locus_tag="OO609_002297"
     CDS             18939..19193
                     /locus_tag="OO609_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6633694.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19243..20703)
                     /locus_tag="OO609_002298"
     CDS             complement(19243..20703)
                     /locus_tag="OO609_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6633695.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20640..21197)
                     /locus_tag="OO609_002299"
     CDS             complement(20640..21197)
                     /locus_tag="OO609_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6633696.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21223..22077)
                     /locus_tag="OO609_002300"
     CDS             complement(21223..22077)
                     /locus_tag="OO609_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6633697.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22077..22886)
                     /locus_tag="OO609_002301"
     CDS             complement(22077..22886)
                     /locus_tag="OO609_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6633698.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(22896..23654)
                     /locus_tag="OO609_002302"
     CDS             complement(22896..23654)
                     /locus_tag="OO609_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6633699.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23651..24631)
                     /locus_tag="OO609_002303"
     CDS             complement(23651..24631)
                     /locus_tag="OO609_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6633700.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24631..25653)
                     /locus_tag="OO609_002304"
     CDS             complement(24631..25653)
                     /locus_tag="OO609_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6633701.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6534 a         6391 c         6758 g         6423 t
ORIGIN      
        1 gccgggggga ttacttataa aggggcatgg actcaacaaa ccgcagaaat ctacagctgg
       61 ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc aaaacctggc atcttaaaga
      121 gtaaaagtat gttttgtgcg atttatcgaa gcagcaagcg tgaccagacc tatttatatg
      181 tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc
      241 ctcagttagc gatgattctg ccgttggatg ggcggaagaa actggtcaat gctgatattg
      301 aaaaggttaa actggcatta accgagcaag gttactattt gcagttaccg ccaccacccg
      361 aagatttgct gaagcaacat ctttccgtca tggggcagaa aacagacgac actaacaaat
      421 aaccgatatc cggcggtggc attatctttg tcggcgcggg ttttcatatc cacgataagg
      481 tgaggggaac gttatgtatc aacatcacaa ctggcaaggt gcgctgctgg attatccggt
      541 gagtaaagta gtctgtgttg gcagtaacta tgccaaacat attaaagaga tgggcagcgc
      601 agtgcccgaa gagccagtgc tgtttattaa accagaaacg gcactgtgcg atctgcggca
      661 gccattggcg atcccatccg atttcggttc agttcatcat gaagtcgaac tggcggtgtt
      721 gattggcgcg acgctgcgtc aggcaacgga agagcatgtc cgcaaagcca ttgccggtta
      781 cggcgtggcg cttgatctga cattgcgtga tgttcaggga aaaatgaaga aagccgggca
      841 gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg ctttccgggt ttatccccgc
      901 ggcggaattt accggcgatc cacaaaatac aacgctgggc ctgagcgtaa acggcgaaaa
      961 acgccagcaa ggtacgactg cggacatgat ccataaaatc gttccgttga tcgcttatat
     1021 gagcaagttt tttaccctca aggccggtga cgttgtgttg acaggcacgc ctgatggcgt
     1081 cggcccgttg caaagcggtg atgagctgac agtcactttc gatgggcatt ctttgacaac
     1141 tcgcgttttg taatactttt tgccgccaga aagcggcggc aaaacttgca tcgctgtgcc
     1201 agactggtta taaggtgcgt tttaacgtaa tggcggaaca cctgatgagc gatgtacctt
     1261 tctggcaaag taaaaccctg gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg
     1321 gttgcggtca gtgttgcttg cataaactga tggatgaaga caccgacgaa atctacttca
     1381 ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca atgtcggaac tacgaacgtc
     1441 gtttcgagtt tgaacccgac tgcattaaat taacccgtga aaatctgcca acattcgaat
     1501 ggctgccaat gacctgcgct tatcggttgc tggcggaagg taaagattta cctgcgtggc
     1561 atccgctact tactggttcg aaagcggcaa tgcatggtga acgtatctct gtgcgtcata
     1621 tcgcagtgaa agaatcagaa gtgattgact ggcaggatca tatcttaaat aaacctgact
     1681 gggcgcagtg ataaatttaa tagatgctga tgggggcttt cttaatatac gtctggatgc
     1741 tcgccatttc gtagccctgt ttttgaaagc gctctgtcat aatgagagtt acccgatacc
     1801 ttattatcaa caggtaactc tcaatcttta aaaaacgtct gtgtttttaa atgtgataaa
     1861 ccttatacct tgagtacatg gagagaataa tcgcttatca gacttcaggt acttcaaaga
     1921 gtgtcttttt accgtgtctt ttctgatact cattacaggt gttaatcatt tttttggccg
     1981 ctgcttttag caaagaaagc attaaatcat cataatcaac atagaatctg gttgtttcag
     2041 tttccgtttt tatctccccg atggcggcta tttcggtggt taatttcaat ttggcggcat
     2101 cgatatcttt attcgcttgt ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag
     2161 attttaactt gttcttcaat tctggaatca gtttcccttc aactacgccc gcagcaatag
     2221 aataggaaat gattaaacca aatggaccgg cgacgacatc ggctgcggca ccggcatacg
     2281 cttccttcct gattttatct acctgtgact ggaaatagct gcttttttct gaaaaatcat
     2341 tggttaactg gctatctaac gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc
     2401 ttaccagcag ggatttttgc gcttcattca gcttcgtgat gccgtcatcc agtaccttaa
     2461 tgaggggcgg atgctttctt ctcattgtac tcatcaaata gcaaaatata cgctgcgagc
     2521 aattgcgtcg caacaccaca ccattcatac actgtttggg ttgcttcaaa atacttatcc
     2581 tggctatcca taagtaaggt tttaatatcg ccgactaaaa cggaggctgc ctgtgaatac
     2641 tcctgtttaa agcgacttaa ctcttttatg gtttcatcaa aggtctgcca ggggatgacc
     2701 tgatcgagat atttattata aagatctaat gctccatctg cggtttcgat tgcgttttta
     2761 actacttcta ccgttttatc tgcaacgatt tcagtcataa tcattcgcct ctttaaatat
     2821 ataaattgta atgaaactcc tgttttacaa ctattaataa attttacttc atctaattca
     2881 tagttagccg ggcgggatgc gtcaatgtct ttatttctat taatatgata aatatcaaac
     2941 aatgtttaat gtcattatgg cgaatgcttc tattctattt ttttgccggg tgatattttt
     3001 catttctgct ggatgagcgt cgtcgccaga aggccacgtg agcacaggat aagagaacga
     3061 aaaatcagca gtctatgcag cgacaaatat tgatagcctg aatcagtatt gatctgctgg
     3121 caagaacaga ccactgtata taaaaacagt ataacttcag gcagattatt atgttgttta
     3181 tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat cttgttcagt
     3241 gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg aatcaactgt
     3301 tgatccagca tcccagcgcg acttacttcg tcaaagcaag tggtgattct atgattgatg
     3361 gtggaattag tgacggtgat ttactgattg tcgatagtgc tattaccgcc agccatggtg
     3421 atattgtcat cgctgctgtt gacggcgagt ttacggtgaa aaaattgcaa ctacgcccga
     3481 cggtacagct tattcccatg aacagtgcgt actcgcccat taccatcagt agcgaagata
     3541 cgctggatgt ctttggtgtg gtgatccacg tcgttaaggc gatgcgctga tgtttgccct
     3601 ctgtgatgta aacgcgtttt atgccagctg tgagacggtg tttcgccctg atttatgggg
     3661 taaaccggtg gttgtgctat cgaataatga cggttgcgtt atcgcccgaa acgctgaggc
     3721 aaaggcgctg ggcgttaaaa tgggcgatcc ctggttcaaa caaaaagatc tgtttcgtcg
     3781 ctgtggtgtg gtttgcttta gcagcaatta tgagctttac gcagacatga gcaatcgggt
     3841 gatgtcgacg ctggaagaac tatcgccccg cgtcgagatt tacagtattg atgaagcatt
     3901 ctgcgatctg acaggtgtgc gtaattgtcg cgatctgact gattttggca gagaaattcg
     3961 cgcaacggtg ctacaacgta cccatcttac tgttggtgtg gggatcgccc agaccaaaac
     4021 gttggctaag cttgccaatc atgcggcaaa aaaatggcag cggcagacgg gtggggtggt
     4081 ggatttatca aatctggaac gccagcgtaa attaatgtct gctctccccg tggatgacgt
     4141 ctgggggatt ggacggcgga tcagcaaaaa actggacgcg atggggatca aaaccgttct
     4201 cgatttggcg gatacagata tccggtttat ccgtaaacat tttaatgtca tgctcgaaag
     4261 aacggtgcgt gaactgcgcg gcgaaccctg tttgcaactg gaagagtttg caccgacgaa
     4321 gcaggaaatt atctgttccc gctcgtttgg tgaacgcatc acggattata cgtcgatgcg
     4381 gcaggccatt tgtagttacg ctgcccgggc ggcggaaaaa cttcgcagtg agcatcaata
     4441 ttgtcggttt atctccacat ttattaagac gtcaccattt gcgctcaatg aaccttatta
     4501 cggcaatagc gcgtcggtaa aactgctgac gcccactcag gacagcaggg atatcattaa
     4561 cgctgctacg cgatctctgg atgccatctg gcaagcgggc catcgctacc aaaaagcggg
     4621 cgtgatgctg ggggatttct tcagtcaggg agtcgcgcag ctcaatttat tcgatgacaa
     4681 cgcaccgcgc cccgggagtg agcaattgat ggcggtaatg gatactctga atgctaaaga
     4741 gggcagagga acactctatt ttgccgggca ggggatccaa caacaatggc agatgaagcg
     4801 agctatgctt tcaccacgtt atacaacgcg aagttctgat ttactgcggg tcaaataaat
     4861 atagcggcag gaaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacagat
     4921 cacgtttttt cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag
     4981 cgataaaaat accgagcagc cactgcggca tttccatacc taaaaaatcc cactgacgct
     5041 cggcgcaatc gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt
     5101 ccgggaaacg aaccataaaa tcacaggtgg caaatggcga aggatagagc tgaagcatgg
     5161 tgtgctcgta agttaactgc acaccgcgga acgcactata caaccagata accatcgcta
     5221 cataacgcag cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga
     5281 ataacgcgca gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga
     5341 accacagcgc cgtcagttcc agtgccagag cagtaaacgc catcaacagc cacgcgcccc
     5401 ggccttgtga acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga
     5461 gtgcgcagtt taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca
     5521 attggacaat tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat
     5581 caaagcgttg ctatccagcc catttgcata aaccattcgg ttacaggggc aagcgtaaac
     5641 tcgacgcaga gcaagccgac gagtgtcagt acgagggtgt aaggcagggc catccacacc
     5701 atgcggccat aagagaggcg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac
     5761 gcagcctgac cgttcggcgt agcgacagag ggcagattgg taccggtatt aatggcgacc
     5821 gccagcagct cgtattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg
     5881 ttgatataaa tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa
     5941 atatagaaca gcgacagctg agcatgttca gatgcctgca acacaaactg aataatcggc
     6001 gaaaacagtt gttggtcgat aatcaccgcg actaccgaga aaaacaccgt caacagtgcg
     6061 gtgaatggca gagattcggt gaaggcttta ccgatagcat gttcatcggt gacaccggtc
     6121 aatgatgttg ccagaataat gactgacaaa ccaatcaagc caacttccgc cagatgcaac
     6181 gccagcgcag tcaccagcca gacgccaata atcgcctgga caatcagacg gattttatcc
     6241 tgacgggtac gctggtggcg gctttgatcg tcaaactgtt gcaacacttc gcggactttc
     6301 tccggcagcg tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac
     6361 aggccacaaa tcagaaccgg aacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa
     6421 tgccagccag ccgctttagc gatgatcagg ttctgtggtt cgcccaccat ggtcattacg
     6481 ccgcctaatg cggtgccgac accggcatgc atcatcaggc tacgcagaaa gccacggaac
     6541 tgttccagaa ccacgttgta atgcttgtcg atatgactat cgtcttgcag gtcggtgtct
     6601 tcggtacggg aagaggctac gcgatgataa ataccataaa aaccgactgc aacgctgatc
     6661 accaccgcca cgacggttaa ggcatcgagg aacgcggaga ggaacgctgc cgccacgcaa
     6721 aaagagagcg acagcagcat tttggagcga atgcttaaca gcaaacgggt aaatatgaac
     6781 agcaacagct gtttcataaa atagataccc gccaccataa acatcaataa cagcaagact
     6841 tcaagatttg ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatacc
     6901 gcttcgatag ccaacagacc accggggagt agcgggtagc atttcagggc catcgccaga
     6961 gtgaaaataa attccgctac cagcaaccag cccgcgacga aagggctgat gaggaaaatt
     7021 aacgggttta cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc
     7081 aaaaaattgc gccatagcgc gcggccccag gagatctcca tgatggtttc ccttacctta
     7141 caaataatca atgatgtttt tatgtttaaa cgcaaagctt aacggtcagg caggagtgag
     7201 gcaagtcttg atagtcaagg ggaaagagat gcggaaaatg aagccttgat ccctttttct
     7261 tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg
     7321 ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa
     7381 gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca
     7441 gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag
     7501 cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat
     7561 ttctgggaaa cttccggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt
     7621 gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac tatttttatt
     7681 cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa
     7741 gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg
     7801 tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg gctgtatacg
     7861 cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac
     7921 cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt
     7981 cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat
     8041 ttagccattc aggggcgata atcccttccg tttaaagagc aaccccctca aacgaggggt
     8101 tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca actcgatgct
     8161 gccgtcttcg ttttgctgtt cgagcatcac atcaaatccc cacaggcgat gcacatgctt
     8221 caggacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatat aacgcagcgt
     8281 cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact
     8341 taagttatat tgcgacgata accggttacg gatctcccga taaccttctt cattatgaat
     8401 agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga agcggaaatc
     8461 acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga aatcacgcat
     8521 cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc
     8581 ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc
     8641 atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga ccacattggt
     8701 gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg
     8761 gttaaggatg gtatagtgcc agaaggtcgc acagccttcg ttcatcacct gagtctgttt
     8821 ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga
     8881 ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg
     8941 atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag
     9001 catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt tctcttcttg
     9061 cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca
     9121 cgagtccaga agccgttcta cttcatcaac gccataacgt tcttcgcact cggtaatata
     9181 tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac tgcggaataa
     9241 gcaattgttt ttaaagaaag agttatgtcc atagcaggcg tgtgccatca ccagcgcctg
     9301 catagtaatg gtgttctctt ccatcaagta agcgatacac gggttagagt taatgacgat
     9361 ttcataggcc agcccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt
     9421 accgaatgac cagtgcggat agttaattgg catgccgacg ctggaatagg catccatcat
     9481 ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc
     9541 cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc
     9601 gctcaaacgt gtggtgtcct tattcataga atcgatcgtc gccatacgcg cacctcattg
     9661 ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg ttaaaaaccg
     9721 cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac taatccgctt
     9781 ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta aagacgccat
     9841 attgccgcag agtcagggag atgtgagtca gctcaccata aaaaagccgt atgttgaata
     9901 atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact gtatctaccg
     9961 ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg
    10021 cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag ccgggggccg
    10081 ccctggaaac cagtgccgcg aatgccgggc aaatctcccc cggatatgct gcaccgtggg
    10141 cagcaccagg tgtgccttta aaagcgatta aatggatgtt ccagcgccat gcgccgctgg
    10201 cggttcgtct cgacggtacg cagtttcagt tgaaatggat gtggcaaatg ttacgtaact
    10261 gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc
    10321 gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg
    10381 ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc gatatcgccg
    10441 tgctggaaga tgccggcgta ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg
    10501 atcccgcgct ggcagaagta gcgcacaaac tgacgggcgg cctgcagtta cccaatgatg
    10561 aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag caggcggggg
    10621 ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag caaatctacg
    10681 gcgtgaagtt tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg tttggttctt
    10741 actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg aaaggctact
    10801 cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt cttgatgaaa
    10861 cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga atggcggaaa
    10921 ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc
    10981 gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc
    11041 cgatgacgcc agacggtacg ccggttgtcg ggagtacacg ctttaaaaat ctgtggctga
    11101 ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag ttgttaagcg
    11161 atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta gcgcgctaca
    11221 gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc taaggaaacg
    11281 agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag aatctgtcca
    11341 ttgtccgcca ggccgcgccg cacgcgcgcg tctggtcggt ggtaaaagcg aacgcttacg
    11401 ggcatggtat tgagcgtatc tggagcgcgc tcggggccac cgatggcttt gcattactta
    11461 acctggaaga ggcaataacg ttacgtgagc gcggctggaa ggggccgatc ctgatgctgg
    11521 aaggattttt ccatgctcag gatctggaga tttatgacca gcaccgcctg accacctgcg
    11581 tacacagcaa ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca ccgttggata
    11641 tttatcttaa agtgaacagt gggatgaatc ggttgggctt ccagtccgat cgcgtgctta
    11701 ccgtctggca gcagttgcgg gcaatggcga atgttggcga aatgaccctg atgtcgcatt
    11761 ttgctgaagc ggaacatcct gatggaattt ccggcgcgat ggcgcgtatt gagcaggcgg
    11821 cggaggggct ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg tggcacccgg
    11881 aagcgcattt tgactgggtt cggcctggca ttattttgta tggcgcttcg ccgtccggtc
    11941 agtggcgtga tatcgccaat accggattac gtccggtgat gacgctaagc agtgagatta
    12001 ttggtgtcca gacgctaaaa gcgggcgagc gtgtgggcta cggcggtcgc tatactgcgc
    12061 gcgatgaaca gcgaatcggc attgtcgccg caggatacgc cgacggttat ccgcgccatg
    12121 cgcctaccgg tgctcctgtt ttagtggacg gcgtgcgcac catgacggtg gggaccgtct
    12181 cgatggatat gctggcggtt gatttaacgc cttgcccgca ggcggggatt ggtacgccgg
    12241 ttgagctgtg gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg
    12301 gctatgagtt gatgtgcgca ctggcgttac gcgtcccggt tgtgacagtg taacttgttg
    12361 taagccggat cggaggcagc gtcttctggt tgcgaaaaaa tcatccatcc ggctggtcag
    12421 caactgtagt tgttaatgtg acagagccat tgcccatgat agtgtccatt aaaagaatgg
    12481 acactatttc cccggaacct gaactcgccg cacaggcgtt ctacataaaa cgcttacgct
    12541 tcattgttga ctcggagtcg acttcagata aatcacgctt cacccttgat gcagattcgg
    12601 gttcatgttc agattgttga ttaacaggct gcgagtcctg ttgtctttcc agactcaaca
    12661 ggcccaactg ttgatccagg agctccaggt cctcctgtac cttcacgcgc tccaggagtt
    12721 ccgggtcatc caggaactcc agatccataa ggtcctccag ctcctcgagt ccttccaggt
    12781 caacctagcc ttccttgtgc aaaaagtcca aaaggtcctt caggccctgc tgttttttca
    12841 ggccctcgac ggctttgagc agcgtcggta aattgtcttt gtcccacggg ttaccattac
    12901 agtcaaacac cttcaggctc tggggctgcg gcacctgtac cgttttaaac ttgttttctc
    12961 cacagtagta aattattagt ttgggcggca gccgtggtga agagatcaaa tcgttactag
    13021 aacagtcaaa tactttcagg cgagtcaagt tctccagcat ctcatcaggt attgagttaa
    13081 tattgttgcc ggcacaggat agtttataca ggtactgggg catcgacggc ggtaaaaatt
    13141 gcaagaagtt ttcagaacag ttaatcactc tcaggttagt cgacagctca ggcagccaga
    13201 taatactgtt ttcatagcac ttcagtacca tcagttttga gggctgcacc ttcggtaaca
    13261 agatcaagac gttatctgta cagtcaagca ccagcagcca ctttggcatc accttcggta
    13321 acgcggtcaa cacgttttta ctgcagtaaa attcaatcag atgtggaggc aaattctcag
    13381 ggagtgaagt caatcccagg ttgctaagat tgagcatact ccgtcttgta atgagacatt
    13441 ccttcattct gcgcaatgct tctgcccggt tttcaatccg gcttgctggc aggttttctt
    13501 ccaggatatt ttccttgatt tcttccagac tttctttcag gtcttcttgg ggaatttctg
    13561 actgtttgtt ttgctggttt tcttccagga tttcgtacag gtttttttgc aggttttctt
    13621 gcaaaccatc ctgctgtata gcccattcgc accatttggc gaccatcatg aactcgttat
    13681 gttgctgatt cagacgagtc cccaacgtga tgatggtatt gttgtccacc tgtatgttca
    13741 ctttgcccgc gatactgagg tcaatgatct gccagggggc atcaccccag ctgaattttt
    13801 gcgggctgag atacttcatg ttctgcaaaa tttgattctt gagaagagcc gccctggcgg
    13861 gcgtcgtacg cctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc
    13921 tttaaagcta gccataatgc tacgtttcct tgaaataaat atcctctggc agcgccggag
    13981 acgtgaatgt ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc
    14041 atcgttgact tttttcctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg
    14101 gcctaggtag ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt
    14161 tattaagcaa aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga
    14221 aacgcctatt gtgtgggcta tgtcagtgcg gcaggttgcg tgggggaata tgttcaggcg
    14281 atgtcaggca ggggcccctg atggttgacc ggtaatgcgg ggctggtttt tattacctgt
    14341 tacagtcgga aaaagccctt acgctgactt tcttgagccg gtgttttgag ttcattagcc
    14401 agagcctgca atggtttgtc atccataatt taaccttctt ctgatgttgg gatgagcata
    14461 tcaaaatcag gaaatgacaa gtttattaca ggctcaggct gcgttcagta aagcatcgca
    14521 tccggcgata gatgctgcat taaatttaag attcagcttc ctcttcagcc acccgaacgc
    14581 caatcttcaa cacttcatta tcttctttct cggccaccgt ccagatcatc ccggcaaact
    14641 ctacctggtc accgacaacc ggtgccgcgc ctagcaactg ctggacaatt tcacccagcg
    14701 tttgctgttt atcacgatat tctcgcccgt cttccaggcc atatatcagc gcaacatcag
    14761 catatttggc gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg
    14821 gtggcgactg gctgaacagt ttaccgagcg ccgggagatc gcgttcccga ccaattacac
    14881 acaatacatc gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac
    14941 gaaacagggc tgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt gccgcaccta
    15001 cacaccattt atcagcactc aattgataaa caaactgctc ccacggattt tccggatgaa
    15061 tatccaggcc aacgcgtgac accggacgtc cgacgggcgg aaccaccact ttggcttttt
    15121 tcgccgccca cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg
    15181 cgacattaaa gaacagtcgc gcattctcca gccccgccat catcgggaac acggctagga
    15241 tgattggcac cgcgccgcgt aatcccaccc agctgataaa cacgcgctca cgcagattga
    15301 aaccgcggaa agggagcaat ccagcaaata ccgaaagggg acgggcgaag aatatcatcc
    15361 atgccgacaa aatgagcgcc ggaatggcaa taggcagcag atcgcttggg ttaaccaata
    15421 gccccagcac caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca
    15481 ggatgccgta gcggttgcga atcggccgat tacccagcag aaaaccgcac agataaacag
    15541 ccagaatacc gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta
    15601 atgccagcaa tggatataat ccggcaggca gggcgatgcg attaatcatt tgcagcagta
    15661 aataaccacc gccaagaccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacca
    15721 cgaacatcca actgacactg ctttcatgct gctggatcat tgcaatcagg gtaatcgtca
    15781 gaaagaccgc cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt
    15841 cgttaagccc cttaccaccc agcagagaaa agaccgctgc agcatcggtg gagccgacga
    15901 tagcgccgat taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc
    15961 cggttaaacc agaggtgata agtacgccca gcgtcgccag cgacagcgcc ggtcctaacg
    16021 ccacacgaaa ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagcg
    16081 ccaggttact taccatgtag gcgaaggggt aattatcaaa cgggataccg ccgacgccat
    16141 cgactcccgc cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg
    16201 aagaaaatga actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc
    16261 taattattgt tgtggcatcc aatggtcggt tactcctgat tacgctgtct cttatataaa
    16321 ccctaccata ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac
    16381 attttaagaa caggatgacc gctaatcgtc aactgagcgc cttcccgggt gttattcaga
    16441 atggcatgtg cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg
    16501 gtaatgagtg gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa
    16561 cctgcgtcat aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga
    16621 cgtggcaaaa ttcccgcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc
    16681 acgttaatat cttccagcac cagaataccg ttctcaattt ttggcatcca cggtgtacca
    16741 atcagtgaaa tcagcatcgc aagattgcct ccccacaacg tgccttcggt ctgacatgtc
    16801 gggccttccc cttgccattc aatggtgaag gtttcattgc gtaacgccag ccagaaatgg
    16861 tgctcggtaa aggcgttcag ttcatccgca ccaaagtttg ctaccagcat ggggccgcta
    16921 aaggtgatga cattgccttg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt
    16981 ccgcaaatga gtaacgggtc atgttgttgg cgagccacca gtgcctgcca gtcaatatct
    17041 gccagtaaac gactggcacc gtaaccgccg cgtacagcca gcacgatggt gttgggggta
    17101 gtcagtctgg caagggaatt gagatcttcc agacgctccg tttccgtacc cgcaaaacgc
    17161 tcacaacgac gggcaataac ctcgacgtta ttgacttgat gccccgcgtc ggtaaggcgc
    17221 tggataccac gcagcgcggc gtgctgttta atgctgtaac ccgatggggc aattaagtga
    17281 aacagagaca tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag
    17341 gtgtgccgtt gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga
    17401 gatggtttgc ctttttgatt gtgttattag cgggttgttc atcaaagcat gactatacga
    17461 acccgccgtg gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc
    17521 agaaagccgg tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt atcgctatcg
    17581 aatcgggtgg taatccgaac gcggtgagta aatcgaatgc cattggtttg atgcagttaa
    17641 aagcttcaac ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga
    17701 ccagcgagct gaaaaatcca gagcgtaata tttcaatggg ggcggcttac ctgaatattc
    17761 tggaaaccgg cccgctggca ggcattgaag atccgaaggt actgcaatat gcgctggtgg
    17821 tgtcatacgc taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg
    17881 cgatcagcaa aatcaacgat ttggatgctg acgagttcct cgatcacgta gcgcgaaatc
    17941 atcctgcgcc gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt
    18001 aaatcagtcg cgcactttgt ccgctttttc ccgggcttct cgctcgagag agaaaataat
    18061 ccgctgtaat tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg
    18121 agtggtgatg gtttcattct tgccatcaat cactttgcgc tcgctggtgg agattaactg
    18181 ggcgtcaaag tgaaaaacac cccattgccc catgttgact tcaatttgag cgaagcgcat
    18241 accttcttgt aattcggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga
    18301 caaatcatac aagcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata
    18361 aggcggatgg agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accataaggt
    18421 gggaggcggt acggtaataa atgccggaag ctgcaagtat tcactctgct gtagttgttc
    18481 aacagtaaac tcgactttcg caccctgagt ttcggcggta atggtaatgt gctgtgcctt
    18541 tagcacggcg atgttgtctt cggcttgact gccgaaatcc agcaccagtt tatccggggt
    18601 tattgccagt attttgctga tcagctgccc gccattccaa ctgagacgca aaggaattgc
    18661 ggctttgtgc aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg
    18721 ctcatggtaa tgactcacgc ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg
    18781 tcatagttaa caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc
    18841 agccgacaaa cgcccagcat cgatcccgcc cttgcctata cttagagcgt tgacgtaagc
    18901 atttcttgcg accgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta
    18961 tttttggcct gatagccggc attatcggca agctaatcat gccggggcgt gatggtggtg
    19021 gatttttcct gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga
    19081 ccatgtttgg cattgacggc tccatcagcg gttttaattt gcacagcttc ctggtggcgg
    19141 tggtgggcgc tatcctggtg ctgggcgtat tccgcctcct gcgaagagaa taagattttc
    19201 ataaggcgga tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgac
    19261 acctgcccag aaattgcgac cttcttcaat aaacccttca ctgtaggcat attgggtatc
    19321 aaacaggtta ttgaccgacg cattgacgct gaagccatga cctaaggtgt aatcggctcg
    19381 aatgtgggtc accgcaaaac cggcggcttt ttgtgaaccg tcactgttgc tgtagctgga
    19441 ggaacgcgcc tcttccgaca gcgttacgct taacggctcc catggtttga gtgtcatcca
    19501 tgcggtcatt gtctgcgttg gaagatcggt tatcttgccg atgtctttgc gtttggcgtc
    19561 agcgtggatc agagcgtagc tcaatcctac atccagtata ttgctgattt tccccttaat
    19621 accggcatcc agaccgctgt aatccaccgt gccgctgttc tgattttgaa tggtatcggc
    19681 attgatattg tgcgagagga tggcatcact cacccggtta tagtaaacgc tgacctcaaa
    19741 tccccagtcg cgcgtgaagg caccattcca ggttaaatcc accccgcgag cgcgttccgg
    19801 cttgagctgc gggttaacaa tcgctatctg gttatacgca ggtttggatg tggtatagcg
    19861 ttctttcagc gtcggaaagc gtgtgcggtc atagtacgaa agcgccagag tgtcttcatt
    19921 ggcaaagtgg tatttcccca tcacctgcca gttaaaagct gactgattgt tgtcgtcata
    19981 atgggtgatg ctgccatctt tctcgtgttt tttcgcttct acgctatcgc gccagtcata
    20041 gctgattccc gccacgacat cgacattatc agcagcagcc cattgatatt cactggcgag
    20101 cgaccaggta cgatcttcat agcgatcgta agcggcgtgc ggcgcacctt tttcccggtg
    20161 tacgtcatct ttccagttaa cggcaaacga cagcagatcg ttttcacgca catcggctgc
    20221 cagttgtaat ccggcaccgt cgctgtaatc ggaataatgg ctgtagctgc cttttttatt
    20281 tttcaaatca gccagcgagt tgtacatcat cagcgtattt tcaaaggtgt cgcgatacag
    20341 ccgacttttc agggtaaaac ggtcgtttag ttgggtcgct ccctgataat aaaaactttc
    20401 tttgtcatac tctggccact gccagtagcg tgatttttga ccacaatttc cgctgtatgg
    20461 cgggttatct ttttcaccat cctgcttaat gtaagtcagc gtgtattcat cgttttcgcg
    20521 cggtgtaaaa ccgagcttca caatgccgcg tttatcatca gccgatgaat taatcatctt
    20581 gccgtgtttg cctgcaatat cattatttac accatgcggc aggccgagaa aatcctgctt
    20641 tagctggcta ccgctgactt gcaaataccc aagatcgctg ctggcggcaa atgaagcatg
    20701 catatcgtag gcattgtcct gactacggct ccatccctgg cgatatccca gacttgcttc
    20761 ttcagggtgg tgatattaat ggctccgccc atctgattag gcccatgaag cagcgacgaa
    20821 taccctttgg aaacttcgac tgcccccaga ttgttggtca gaatccgcgc cagatcgagg
    20881 ttgccgtcat aggggacata aatgggcaca ccgtcgaaat agaccggcac ctgccgacta
    20941 tcaaagccac gaacttttac ctgttcttcg ttgcggctgc ctgacttttg cagcaccacg
    21001 ccggggacga cacttaatgc ctgagcgaca ttctgtttat ccaaggcctt catggtgggt
    21061 tgatcgagaa cggtggtcgt cgtcgatgat accggactgg accatacggt cagcgtgtcg
    21121 ctctcctgag ccgctacggc ctgctgggta aaggcaagtg ataatgctgt gcagagataa
    21181 cgttttttga gtctcataga ataaattccg tttaacaaag aattaaatag aggctgtggg
    21241 ttgcagactg actttaatgt cagctggtgc cgcgtaataa ggcgcggagg taataaaaag
    21301 gcgaatgccg cagtcgaggt aatttttgag tgtcgtcaga ttaattccgc cggtcagagc
    21361 cagcgtgcag tgaggagcca gcgacggcgc tatttgcgca atttcagttg cctgttgcgg
    21421 actaaatttg tcgagctgaa gtacgtctgg ttgtgcgcgt aacgcggcga tagcctcttt
    21481 cggcgtgtcg gcttcaacaa caattttctt ctcgggtgcg tggcgacgta actgagcgat
    21541 tgcgcctgac cagccctgat tgtcatgaag aaaatggcgg tggttggcaa acaataatat
    21601 ggtttccgca catccggcac gatgaattag tcctccggca gccagaattg cctgcgaagc
    21661 cagtaagcgg gtgcccggaa ttgcttttcg ggtgcaggcg atattgccgt caggataacg
    21721 ttcacgaagc aatgccagca tttgagcgag ataatcagaa acaccgcaac tccactccag
    21781 cacattctgg actgccttcc atccctgatg aagtgctgcg gcattaccct gcgcacggat
    21841 tagccgttga cccgcgttcg cttgtgaacc gtcgctgacc gcgtcatcaa tagttaatcc
    21901 cagcgtagtt aacatcttac acgcgacaga aacgccgctg acgcagccgc cctgacggtg
    21961 aaaaaacgct atatagccag gctggtgtcc aatatttaat gctcgcgtgg tcaggtcacc
    22021 tccctggata tcttccagca gtaacgcatc gatttgtgcc tgagaaagga aaatcatcac
    22081 agtgcctcct gcggtacgct atcccaccag atgagcgccc agtcgcggct ggcaggggcg
    22141 agggcacgag catcttgttg ttgataccag tgatataacc gctcacgctc atcgtcgtta
    22201 agcgcgccaa gactccagct gacgttctgc tcaaaacgtt cccaggtgct gttatcctgt
    22261 tggcaatttt gtccacggat aaaatcaacg tgggcataaa ttcccatctg atacaggacg
    22321 ttgagcgcaa agatatagtt gggtaattcg atcacttccc gaccagccgc ccgctgaatg
    22381 gctggagaga cgaacgaggt gctgaccaga tgagttgtat agacgcgaag ccgtgcctga
    22441 ttattcagtt tgctcattgc atggcgcata tcagccacca gagtggagcg ggaagccacg
    22501 gcaatatcgc agcgtggcag gtcactccag tcctcttccc aggcgcgctg gatccagtgg
    22561 acattgtcag ccttcagtgc ggcggcgcgg cgggcggcaa cgttcagcat cccctgactg
    22621 taatcaacgc cataaaccgt agtgagtttg tcagccagcg ccagcgatac ggtgcctggt
    22681 ccgcagccca tatcgaataa ggtctgagcg ccttgcaggt caattttagc gatcagttgt
    22741 tgcaggtagc tgtccgttgg cgaggcgcaa ttttccgcca ttttctccgc ccgttgatcc
    22801 cagtgatcgg gcgttttttc tgtgcggtga gccagtttga gttgctgcag ataaaggtct
    22861 gcaaaatcaa tatcatcaat gagcatgatt gttccttagc ggctaattgc agagaggtga
    22921 tgatgaattt ggtcggcaga aacccgatac agggccgcca gtttgttcgt tgttaactgt
    22981 tcggtcggta agccttgtgt tactcgtcca tctggctcta cttggataat gctgtcggca
    23041 atggcgttgg catggagcgt atggtgagtc gacattaaca ttgtcatgcc gttatttttg
    23101 agctgcgcca gcgtgtccag aagttggatc tgatgaccga aatcgaggct ggaggcgggc
    23161 tcatccagta acaacaagcg cggttgttgg acgagtgcac gggcgattaa agccagctga
    23221 cgttcaccgc cgctaagcgt attccagcgt cgtgtcgcca gatgcaaaat gcccagcttt
    23281 tccagttgct ccgtggcttt caggcgttct tgcttaccgg gaacggaaaa agcgcctatg
    23341 gtgggggcca ggcccatcaa caccatatcc agcacactga aagcaaaaat accgtcgtgc
    23401 gcctgcggca cccaggcaat agcctgtgcg cgctggcgat ggctgagtgt ctgcacagag
    23461 aggctatcca gtaggatctc gccttttaat aacggtataa cgccaagaat ggagcgcatg
    23521 agtgtggttt tgccgcaacc attcgcgcca agcagacaac aaattgttcc ttgcgacagg
    23581 gtgaaggaca cgtcgcgtag cactggatgg cgggagtaac ccagagacgc atgacgaacg
    23641 gcaagcaaag tcactggcga cctccgcgta acaacaaaag caggaaaaac ggtgcgccaa
    23701 caaaagcggt cagaatgcca agtgggattt ctgttgttcc aatgctgcgc gccagcgtat
    23761 cggtaagcag gagcagaata gcgccagtac acattgccat aggcaacagc tgctgatggt
    23821 tatgaccggt caacaagcgg gcaatgtgcg gaacgaccag accgatccaa ccaattatgc
    23881 ccgctattgc taccgtgctg gcggtgatta gtgtggcgca gacaatcaat ccaaagcgca
    23941 gacgcgtcac gttcagtccc agcgaacggg cttcgtcgtc ggaaagcgtg agtaaattca
    24001 tgcgccagcg gagaaaaaac aggggcaagg agcctatgag aatgatcggt gcagcatagc
    24061 agagatcgcg taaggtcacg gtggacagtc cccccagcaa ccagaaggta attgacggta
    24121 actgggtgta aggatcggcc agcgttttaa ttagcgatat ccccgctccg cacaacgttc
    24181 ctaacgcaat gcctaccagc accagggtta agatcggatc gtggcgggtc acccgacgcg
    24241 taatcaacca gacgcccgcg accaccatca gcccgccgca aaatgcgtac agctggatgt
    24301 aaacaatggg taatccccac aggattgccg cgcaggcacc aagtccggca ccggcggaga
    24361 cgcctaaaat atccggtgaa actaagggat tacgcaacat tccctgatag gtggttccgg
    24421 ctcctgccag cgctgcgccc agtaacagcg cagcaagaat acgcggcacg cggatttgcc
    24481 agaagacaat ttgttcctgc acagactcat tttcttgacg aagaattagc gccagcagtt
    24541 gctgcatatc gagatgataa gccccactta cggcggcaaa agtgatactg atagctgcca
    24601 gcagcaaaat acatccgttt acgattctca ttagctcgcc accaattttt gatagtcggc
    24661 atcactgagt gaacaatgcc agaacagttg ggcgtaatgc tgcatgtcac ttttaaactg
    24721 gcgattgatg gcgggatcca gccaggcgtg aagtctgcgt aatcccagaa gacggttgat
    24781 tcccggcggg gcatcgagcc agccaaaggg caagccactt aaaaataaga tgcgttgttc
    24841 cgcaacggct ttcacgccct gccagagagg atcacgacga ataaaatctg cagtaacggc
    24901 ctcctgaacc agaataatat ccggctgcca gcgcaggaga ttttccatgg aaacctgtgt
    24961 caggccgtgg cgatcggcta tttgcgcgac gttgtgcaaa cctaataatt ccgccgcctc
    25021 ggtatgcaac gaaccctgta agcccgtttc cagccctcgg ggaccgcgcg cagcataaaa
    25081 gcggaggtta gcggcgggtg aggtggcgaa tgcctgagct tcaccaacga agtggctggc
    25141 gagattggct tgttctgcgg cgcggtgctc ttcgcctaac gttttgccaa gcgttgttaa
    25201 ctgttctgct gattgcgcca gttttccgtt aagcaataac caggggattt gtgtctgttc
    25261 gctaacctgc cgtgcctggg agatcaaggt ttcatccgta ttgccgcaat caacgaccaa
    25321 atcgggatgt aacgccataa gcccttccag cgatagtgtg ctggcgcgcc cggcgagtcg
    25381 tcccagcctg ggtaactggc gaatgtgctc tggcaatgga attaatgcct gacgggcaaa
    25441 atcaaaagag gaaaaaccga ccattttttc tggcgcgaca gccagcaaga gcaaatcggc
    25501 gggcgcgcct gcgctgacga tgcgtgtaat gttttgggct tcgggaagag attgcgaagc
    25561 aaaacgatta actgtttgtg aaaaggacgg tagagactgc aggagcaggg tagatgcaag
    25621 tgcctgcgca aacgtccgtc tggttatggg catggtgaac atcctgaaaa gtaaaatcga
    25681 tatatacttt catatatatc gataaaaatc atgttcaata tcaaagttta gctattgaaa
    25741 acatgaagat gtgacgactt ccgcaagcct gttgaccctc tctatcatta aaaatgtatt
    25801 tatcaggtgg ctaatattat ttaaatcatt taaggctaaa ttatttaaat atgaatgaca
    25861 ttattgtttt tttatcgtcc acggttacgc gacgttgaag ttacttgttt tataatgagc
    25921 gatttttgat atctcgccga gaaaattatc agcattctct cgtttcagtg tcgtgaatat
    25981 actgttaacg gaggtgaaaa gaatgctggg ggttatacaa aacctatgtt gttgtcgtcc
    26041 ggattcaccg cttgctgata gtaagcagcg taaccagaag ttaccgcaac aagactttga
    26101 ggattt
//
LOCUS       DAJTYH010000057        25677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTYH010000057 DAJTYH010000000
VERSION     DAJTYH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO609_002305"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO609_002305"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6633702.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO609_002306"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO609_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6633703.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO609_002307"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO609_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6633704.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO609_002308"
     CDS             3807..3995
                     /locus_tag="OO609_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6633705.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO609_002309"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO609_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6633706.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO609_002310"
     CDS             4756..5115
                     /locus_tag="OO609_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6633707.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO609_002311"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO609_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO609_002312"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO609_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6633708.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO609_002313"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO609_002313"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6633709.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLNSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO609_002314"
     CDS             10913..11254
                     /locus_tag="OO609_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6633710.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO609_002315"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO609_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO609_002316"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO609_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6633711.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13681..14967
                     /gene="dctA"
                     /locus_tag="OO609_002317"
     CDS             13681..14967
                     /gene="dctA"
                     /locus_tag="OO609_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6633712.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15188..16684
                     /locus_tag="OO609_002318"
     CDS             15188..16684
                     /locus_tag="OO609_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6633713.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16780..17709)
                     /gene="kdgK"
                     /locus_tag="OO609_002319"
     CDS             complement(16780..17709)
                     /gene="kdgK"
                     /locus_tag="OO609_002319"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6633714.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17941..18708
                     /gene="pdeH"
                     /locus_tag="OO609_002320"
     CDS             17941..18708
                     /gene="pdeH"
                     /locus_tag="OO609_002320"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6633715.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18778..20838
                     /locus_tag="OO609_002321"
     CDS             18778..20838
                     /locus_tag="OO609_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6633716.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21020..22342)
                     /locus_tag="OO609_002322"
     CDS             complement(21020..22342)
                     /locus_tag="OO609_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6633717.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22753..23766)
                     /gene="yhjD"
                     /locus_tag="OO609_002323"
     CDS             complement(22753..23766)
                     /gene="yhjD"
                     /locus_tag="OO609_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6633718.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23815..24714)
                     /locus_tag="OO609_002324"
     CDS             complement(23815..24714)
                     /locus_tag="OO609_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6633719.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25234..>25677
                     /locus_tag="OO609_002325"
     CDS             25234..>25677
                     /locus_tag="OO609_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6633720.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQRE"
BASE COUNT         6110 a         6456 c         6988 g         6123 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctgaaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc cccccccccc ataaccttac aagacctgtg gttttactaa aggacaccct
    13681 atgaaaacct ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt
    13741 attctccttg gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc
    13801 ttcgttaagc tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc
    13861 attgcgggca tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac
    13921 tttgaaattg tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag
    13981 cctggtgccg gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac
    14041 gccgatcagg cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc
    14101 gtcattggcg catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt
    14161 ggttttgcgc tccaccgtct gggcagcaaa ggccaactga tttttaacgt gatcgaaagt
    14221 ttctcgcagg tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg
    14281 ctcggggcaa tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg
    14341 cagctgatta tctgtttcta cattacctgt atcctgtttg tggtgctggt attgggttca
    14401 atcgctaaag cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg
    14461 ctgattgtac tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg
    14521 gagaaactcg gctgccgtaa atcggtggtg gggctggtca tcccgacagg ctactcgttt
    14581 aaccttgatg gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact
    14641 aacagtcaga tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct
    14701 aaaggggcgg caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg
    14761 gtgggccatt tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca
    14821 gaagctcgtg cgctaactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag
    14881 tgggtgaaag aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat
    14941 ggcaaaacgc acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga
    15001 gtgcgggtgt ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag
    15061 gtttatttca cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt
    15121 aacgtcattt agactgaccg acaacgggca gtttttttat taccaggata gttgatcagg
    15181 ggttcacatg cagggcataa aaattcgact tttagcgggc ggtttgctga tgatggccac
    15241 tgctggctat gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct
    15301 ttccaacggt ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga
    15361 aattcgcctg ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag
    15421 tcacgccatc cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg
    15481 ttcattgtgg cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta
    15541 tgacaccacg ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc
    15601 gctctcttat ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca
    15661 cgcgctgcaa agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg
    15721 gcgctatcgt ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca
    15781 acccgttgaa gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat
    15841 gacgctactg gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac
    15901 gtttggcgaa ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct
    15961 gcgtgcggaa gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat
    16021 gtgggatacg ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc
    16081 ggacctggcc cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtgacag
    16141 caaagacatc ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat
    16201 caacatcgaa tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact
    16261 ggcgaaggtt cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa
    16321 gaaactggag ctgcagaaac tgtttgccgc ctatgcacgg gctgataccg atattctgat
    16381 gggccagcgg atgcgttcgt tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca
    16441 gaaactgcgg caggatttcc ttaacagcct gactgtggag atgttaaatc aggatctgcg
    16501 tcagcagttg tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt
    16561 taacatgaaa gcgttgcagg cggcctggga tcaaatcatg gccccatcta ccgccgctgc
    16621 gaccacctct gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca
    16681 gtaatgcctg atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag
    16741 aggggtaaca ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg
    16801 cgggataatc gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc
    16861 cgcgttttcc gcactgccgc ccgtcaaacg taccgccaga taaccggcgc tgaaagagtc
    16921 gccagctgcg gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatcaac
    16981 taacgcttca ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc
    17041 tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag
    17101 cgcgtcttcg tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgctggta
    17161 cacctgctgt gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat
    17221 cacttttccg cctttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt
    17281 cgggcttaag atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc
    17341 ttcgcaaatc gccgcagact gctcgctcgc cagccagaat ttggcggcgg cttcgttacg
    17401 ccagtagtag aacgtacgtt cgccggtgct gtcggtttca atgtagtaaa ggcccggaag
    17461 acggttttcc atccgttggg tcagggaagt atcaacgttc tcgccgtgcc aggcgtccag
    17521 catctgctgg ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc
    17581 aggatcgacc tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg
    17641 cttaacgtcc gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt
    17701 tttggacata gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt
    17761 ttgctggtca accatattaa aacagtgttc cattattttt ttgagccaga acgtattcct
    17821 gaaagattcc gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt
    17881 acgcgccgat aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg
    17941 atgataaggc aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag
    18001 gaacggcgtt tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca
    18061 tgcgggcggt taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg
    18121 caacgcctgc cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt
    18181 gtgaaagagc agattgattt gctggcgcaa aaagccgact tctttgtaga gcacggcctg
    18241 ctggcatcgg tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc
    18301 ctgcgccaga ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg
    18361 ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt
    18421 ggtaccggga tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc
    18481 gcgcgagaac tgtttgtgat gctgcgtcag tcgccggaag gccgcacact cttttctcag
    18541 cttttacatc taatgaatcg ctattgtcgc ggggtgattg tcgagggcgt agaaacaccg
    18601 gaagagtggc gtgatgttca gaactcgccc gcattcgccg cacaaggctg gtttctttca
    18661 cgcccggcac cgatggaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt
    18721 tccgccgggc tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg
    18781 agcaaggcag gcaaaataac cgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg
    18841 gcgatcattt tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa
    18901 gtctctgcgg agttgaatcg tccgttcgct atccgtggca atctgggcgt ggtgtgggag
    18961 cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa
    19021 gacatcattc ttggtaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga
    19081 gtagaggcaa cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc
    19141 aagctcgaaa agcccgacgc gcgcctgatt cgcctctctg aaaagaacaa taactggacg
    19201 tttaatcttg ccaacgatga caacaaagac gcgaatgcaa agccgtcggc atggtcgttt
    19261 cggctggata atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa
    19321 gcggatctgg aaatttttgt tgatccgtta ggcaagccgc tgccattcag cgaagttact
    19381 ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt acatttttgg cctgaaggcg
    19441 cagggacgtt ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg
    19501 ctgcgtggcg aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt
    19561 gttgcttttg atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt
    19621 aaattttctg gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat
    19681 accccgccgt ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg
    19741 gtctttgatt atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg
    19801 gtctacacca ccggaaagcc acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg
    19861 cggctggcgg acctgggacc gttgattggc gttgattccg ggaaaggggc agaaaagtcg
    19921 aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct
    19981 tatgaccgct tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggg
    20041 cggcgcattg agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc
    20101 aaaaatgctg acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg
    20161 gcgaatattc atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct
    20221 cgtcgactga aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg
    20281 gaaatgaacg gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc
    20341 aacagtaacg gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg
    20401 gagattgttg ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag
    20461 gtgcgggtga actgcgcggc ggcgaatctg aatattgcca acggcgtggc gcgcccgcag
    20521 atttttgctt tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct
    20581 tcggaacagc tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg
    20641 cgttcgccgc tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga
    20701 ccgctgattg cccgtggtgc tgttgctgcg gcactggcaa cgctggtaac accggcggcg
    20761 gcgttactgg cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg
    20821 tcgcagatga agaagtgatg ataattgccg gatgtctgac atccggcaat taaacagatt
    20881 tagatttttg gcaaagtgtg ctttgttcat gccggatgcg gcgtgaacgc cttatccggc
    20941 ctacaaatcc ttgtgaattc aatatattgt atggaaattc agtcctgata agcgtagcgc
    21001 atcaggcttt tcacacttat tacaacgact gatgtcgcgt ctcatgggtc agcagcaggg
    21061 cgattaacgt caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt
    21121 tagcctgcaa ccaggctgcg atatacggcg caacggaagc cccgagaatc gacgatacgt
    21181 tgtaagagaa cgatgctccg gtgtaacgca cttctgtcgg aaacagctct ggtaacagcg
    21241 cacccatcgg cccgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa
    21301 ccagaatcgg gttgccagaa ccgagcagcg ggttaaaggc gaacagcgcg aacaggatga
    21361 tcagcgtggt gatgatcacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccgg
    21421 cgactggcac catcacgcca aaaccaataa ctgccatcat cagcatccac aacacttcgt
    21481 tacgcggcag gccaagccca actggcgcgg cggcggtact gaaggtcata gagtagaccg
    21541 tcatgatgta aaacagcgta taggttgcca gcataatgaa cgtacccagt acggttacgc
    21601 gaacatgttt ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga
    21661 ctttctcaaa caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca
    21721 gcaccgccga gaagataaat ggcacacgcc agccccagct cataaactgc tcatcggtca
    21781 gtagccagga aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccta
    21841 gctgtgggaa ggagccatac agtgcacgtt tgcgcggtgg ggcgttttca gtcgccagca
    21901 gcgccgcgcc gccccattca ccgcctaagc ccagaccctg accaaatcga gccagcgcca
    21961 gcagcagtgg ggcgaaaata ccaatcgtgg catagcccgg cagcagacca atcaccacgg
    22021 tcgaaatccc catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc
    22081 caaaatgacc aaaaacggca gagccaatgg ggcgcgcgac gaaggcgatg gcgaaggtgg
    22141 cgagcgactg tagcgttgct gccgttggat cgccctgcgg gaagaagata tgcggaaaca
    22201 caataacggc cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggcc gtgccaatga
    22261 gagaggcgac aaggacttta ttacgcgagt tgatcggcgt gtattcttgt tcgtggtcga
    22321 gtgttgtggc tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt
    22381 acgcacagca aaagtgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg
    22441 caaaaagggg ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt
    22501 tataaatagt ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg
    22561 ctggggttat gagttggagt gaaattgttt gctcccaaat agatggctga agaggctctg
    22621 gggaagagtt tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta
    22681 tccggcctac ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc
    22741 atccggccta ttttaaggct gcgttttccc tggcattcgc gggtcgtctt tatattcggc
    22801 ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat
    22861 cagccccagc acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt
    22921 gtaggtcatc acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat
    22981 cagggctttt ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag
    23041 atagttggcg aagatggaaa tcgccaggcc aatcaatcgc cacgtcggtt tcagccactc
    23101 aatgctgttc aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt
    23161 gatcgaaagc gtcacaatca gcgcaatcaa caaaccaatc agtgaaataa aatcacgcag
    23221 atattttacc cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgcgactg
    23281 ggcacgaatc gcttcacgca ggttacccat ccagttgatg ccggaataaa gtgccaccgc
    23341 caggccgaca agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa
    23401 cgtggcggct agtgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag
    23461 caacatcgga tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg
    23521 gatcatcgac aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc
    23581 gttaaagcgt tctgtcgcgc gaataaggtg cgcaataacc ggttggcgtt ggactttttc
    23641 ggcagtggtg gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc
    23701 caactgctta atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg
    23761 cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta aaaagtatag tcggtcagtc
    23821 caggtgctct ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag
    23881 ctcccgacgc tgcgggtaaa ccagcgaaag ggagagcggc tcggcacggt agcctggcaa
    23941 cacttcaata agccgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat
    24001 aatccccaga ccggcaagac cggcggcgag atagttttcg ctactgttta ccgtcaacat
    24061 gccgccagac ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg
    24121 tacacccagg tgcggtgtat aacgcactat tgcgtgtgaa gtcagatcat cggggctttg
    24181 cggataacca aagcgcgtca gatagtgcgg actggcacag ttgaccatgg ttagtttgcc
    24241 gaggggacgg gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa
    24301 accatcatga agaatatcta ccggacggtt atggctactc agttccagct caattcccgg
    24361 atgctgatag agaaattccg acagacgcgg cagtaacagg tttttcgcga ttcctgacgg
    24421 gatgtcgatg cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag
    24481 ttcgctgaga ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt
    24541 gagtttgacc cgtcgcgtag tgcgctggag aagctgggtg ccaagttgat gttccagtgc
    24601 ctgtatctgg cgcgaaacac ttccctttgg caaagcaaag aaatccgccg cgcgggaaaa
    24661 actttccagc tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt
    24721 gaccgattgt tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc
    24781 taacatctaa tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg
    24841 aaaagggcgg aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag
    24901 tgattccctt taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt
    24961 gccatttgtt attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac
    25021 caccaatata aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag
    25081 gcgtttagct gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg
    25141 gccaccgcca aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa
    25201 gtctttttaa cgtattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag
    25261 tcaatattta ttcatggaat aaaaatcagt ttacagcagc gtattccagg agtgagtatt
    25321 cagggggtca gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta
    25381 gttatgctcg atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg
    25441 gtgcagtttc ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta
    25501 caggaagtta tccattttaa tgtgctggcc attgtgcctc gcgattcaac cgtcgagaca
    25561 tttgcgttgg cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg
    25621 acgacgccgg aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggag
//
LOCUS       DAJTYH010000058        25677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTYH010000058 DAJTYH010000000
VERSION     DAJTYH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..249
                     /locus_tag="OO609_002326"
     CDS             <1..249
                     /locus_tag="OO609_002326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633721.1"
                     /translation="LNTDVPLTAGLADSDIFHNTRDLTAVPVAHPAKFWQKNTAVFLS
                     ELNALRIAECIILSPLFESGERRALLEKVFAGLLRVFQ"
     gene            complement(187..492)
                     /locus_tag="OO609_002327"
                     /pseudo
     CDS             complement(187..492)
                     /locus_tag="OO609_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            560..1175
                     /gene="tnpA"
                     /locus_tag="OO609_002328"
                     /pseudo
     CDS             560..1175
                     /gene="tnpA"
                     /locus_tag="OO609_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1503..2585
                     /locus_tag="OO609_002329"
     CDS             1503..2585
                     /locus_tag="OO609_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6633722.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3074..3872)
                     /locus_tag="OO609_002330"
                     /pseudo
     CDS             complement(3074..3872)
                     /locus_tag="OO609_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3882..4433)
                     /gene="ybcL"
                     /locus_tag="OO609_002331"
     CDS             complement(3882..4433)
                     /gene="ybcL"
                     /locus_tag="OO609_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6633723.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4602..4934)
                     /gene="emrE"
                     /locus_tag="OO609_002332"
     CDS             complement(4602..4934)
                     /gene="emrE"
                     /locus_tag="OO609_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6633724.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5279..5593)
                     /gene="fliE"
                     /locus_tag="OO609_002333"
     CDS             complement(5279..5593)
                     /gene="fliE"
                     /locus_tag="OO609_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6633725.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5807..7466
                     /gene="fliF"
                     /locus_tag="OO609_002334"
                     /pseudo
     CDS             5807..7466
                     /gene="fliF"
                     /locus_tag="OO609_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7459..8454
                     /gene="fliG"
                     /locus_tag="OO609_002335"
     CDS             7459..8454
                     /gene="fliG"
                     /locus_tag="OO609_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6633726.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8447..9133
                     /gene="fliH"
                     /locus_tag="OO609_002336"
     CDS             8447..9133
                     /gene="fliH"
                     /locus_tag="OO609_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6633727.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9133..10506
                     /gene="fliI"
                     /locus_tag="OO609_002337"
     CDS             9133..10506
                     /gene="fliI"
                     /locus_tag="OO609_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6633728.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10528..10869
                     /gene="fliJ"
                     /locus_tag="OO609_002338"
                     /pseudo
     CDS             10528..10869
                     /gene="fliJ"
                     /locus_tag="OO609_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10866..11993
                     /gene="fliK"
                     /locus_tag="OO609_002339"
     CDS             10866..11993
                     /gene="fliK"
                     /locus_tag="OO609_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6633729.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12098..12562
                     /gene="fliL"
                     /locus_tag="OO609_002340"
     CDS             12098..12562
                     /gene="fliL"
                     /locus_tag="OO609_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6633730.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12567..13571
                     /gene="fliM"
                     /locus_tag="OO609_002341"
     CDS             12567..13571
                     /gene="fliM"
                     /locus_tag="OO609_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6633731.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13568..13981
                     /gene="fliN"
                     /locus_tag="OO609_002342"
     CDS             13568..13981
                     /gene="fliN"
                     /locus_tag="OO609_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6633732.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            13984..14349
                     /gene="fliO"
                     /locus_tag="OO609_002343"
     CDS             13984..14349
                     /gene="fliO"
                     /locus_tag="OO609_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6633733.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14349..15086
                     /gene="fliP"
                     /locus_tag="OO609_002344"
     CDS             14349..15086
                     /gene="fliP"
                     /locus_tag="OO609_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6633734.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            15096..15365
                     /gene="fliQ"
                     /locus_tag="OO609_002345"
     CDS             15096..15365
                     /gene="fliQ"
                     /locus_tag="OO609_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6633735.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15374..16159
                     /gene="fliR"
                     /locus_tag="OO609_002346"
     CDS             15374..16159
                     /gene="fliR"
                     /locus_tag="OO609_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6633736.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16444..17067
                     /gene="rcsA"
                     /locus_tag="OO609_002347"
     CDS             16444..17067
                     /gene="rcsA"
                     /locus_tag="OO609_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6633737.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17111..17299)
                     /gene="dsrB"
                     /locus_tag="OO609_002348"
     CDS             complement(17111..17299)
                     /gene="dsrB"
                     /locus_tag="OO609_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6633738.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17462..17689
                     /gene="yodD"
                     /locus_tag="OO609_002349"
     CDS             17462..17689
                     /gene="yodD"
                     /locus_tag="OO609_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6633739.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            17987..18802
                     /locus_tag="OO609_002350"
                     /pseudo
     CDS             17987..18802
                     /locus_tag="OO609_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18799..20493)
                     /gene="yedQ"
                     /locus_tag="OO609_002351"
                     /pseudo
     CDS             complement(18799..20493)
                     /gene="yedQ"
                     /locus_tag="OO609_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20664..20846)
                     /locus_tag="OO609_002352"
     CDS             complement(20664..20846)
                     /locus_tag="OO609_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6633740.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(20925..21842)
                     /locus_tag="OO609_002353"
     CDS             complement(20925..21842)
                     /locus_tag="OO609_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6633741.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22015..22935
                     /gene="yedA"
                     /locus_tag="OO609_002354"
     CDS             22015..22935
                     /gene="yedA"
                     /locus_tag="OO609_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6633742.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(22924..23394)
                     /gene="vsr"
                     /locus_tag="OO609_002355"
     CDS             complement(22924..23394)
                     /gene="vsr"
                     /locus_tag="OO609_002355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6633743.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23375..24793)
                     /gene="dcm"
                     /locus_tag="OO609_002356"
     CDS             complement(23375..24793)
                     /gene="dcm"
                     /locus_tag="OO609_002356"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6633744.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24860..25400)
                     /locus_tag="OO609_002357"
                     /pseudo
     CDS             complement(24860..25400)
                     /locus_tag="OO609_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25440..>25677)
                     /locus_tag="OO609_002358"
     CDS             complement(25440..>25677)
                     /locus_tag="OO609_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633745.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6478 a         6581 c         6325 g         6293 t
ORIGIN      
        1 ctgaatactg atgtaccact taccgcagga ctggctgaca gtgacatttt tcacaacacc
       61 cgtgacctga cggctgttcc ggtagcgcat ccagccaagt tttggcagaa aaatacggct
      121 gttttcctga gcgagcttaa cgccctgcgg atagcggaat gcatcattct gtcaccgctt
      181 tttgaatcag gggaacgccg agcgcttctg gaaaaagttt ttgcaggcct gctcagggtc
      241 tttcagtgac tgttgcaatg gctgtgacgg agcatcttta atccattgcg tttcagtggc
      301 atttttccac tcaaccaatc aggaagccat tttcgcgtaa gggatgtctt tgttaccggc
      361 cttatgattc tcattctgac gtgccagagt acgactgaaa acgaaacgac atgcgcctgc
      421 gaagcgcctc atttcacgcg cctgttgacc accgggtctt aaccggaatt tgaatacctg
      481 tagtcgtttc acagggcaaa ttatactttg gtctgtgaaa aaaactgata tccgacgcgg
      541 aaggcactgt gttttcctga tgcatgctca ccgggtcttt gttaccaagt accgacgcca
      601 gatttttgac catgacgcga cagaaaaact acgcacttac ttttcaaatg tatgtgctta
      661 ttttgaagct gaactggttg aaatggatgg cgaaccagat cacgtccatc tgttaattaa
      721 ctatcccccc aaactggcga tatccagtct ggtaaacaaa cagcctcaaa ggcgtatcgg
      781 gtaggttact gcgacgggat cgaccagata ttgctgccag gtattaccgc aaagatgttt
      841 tgtggcgtcc cggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
      901 aatacattga acaacagcaa acacctggtc agatgaaaaa acgagcctta tatccccgcc
      961 ctgaaggacg ggcttttacg gtgcactgga taagcctgga gattatctac acctgcatgg
     1021 acatcattgt actatgcccc gtacatgctc catcacatcc tcaacatcaa agtattagag
     1081 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaatagaac
     1141 taatatcaag cggcgctata ttgcgtatta aataaaacca cctcaattac attaataaat
     1201 ctaaataaat atcatgcaaa taatttccat ggttattttt aggctattta aattaaaaaa
     1261 ataaattatg agtcaatcat aaataacaac ctttaatata atttgactac caaatttata
     1321 ttcgttcaca aaatagtcat gcattgtgtg caatagaaac agttcagata aaaataggga
     1381 tctacttcac aatttaaatg agaaactaaa acttacatct tgaaataatc tcattgattg
     1441 gatgaatatt tgtagtgcag tgacatcatt tttaaataat agttcaaata aaagggctca
     1501 cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcagtactg atggcgatgt
     1561 ctgctcaggc agcagagatt tacaataaag acagtaacaa gctggatctg tacggaaaag
     1621 ttaatgctaa gcactacttc tcttctaacg atgcagatga tggcgacaca acttatgttc
     1681 gtctgggctt caaaggcgaa actcaaatca acgatcaact gactggtttc ggtcagtggg
     1741 aatatgaatt caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat aaaacccgtc
     1801 ttgccttcgc tggcctgaaa ttcggtgact acggctccat cgattatggc cgtaactacg
     1861 gtgtagcata cgatatcggt gcatggaccg acgttctgcc agaattcggt ggcgatacct
     1921 ggacccaaac agatgtgttc atgactggtc gcaccacagg tgttgcaacc tatcgtaaca
     1981 acgacttctt tggtctggtt gatggtctga actttgctgc tcagtatcag ggtaaaaatg
     2041 accgcactga cgtaactgaa gctaacggtg atggtttcgg tttctccact acttatgagt
     2101 atgaaggatt cggtgtaggt gcaacctatg ctaaatctga ccgcactaat gatcaggtta
     2161 tctacggtaa caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg gcagctggtc
     2221 tgaaatatga tgcgaacaac atctatctgg ctaccaccta ttctgaaacc cagaacatga
     2281 ctgtttttgg taataaccat attgccaaca aagcacaaaa cttcgaagta gttgcacaat
     2341 atcagttcga cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa ggaaaagact
     2401 tgggtgcgtg gggtgatcag gacctgattg aatatattga tgtaggtgca acctattact
     2461 tcaacaaaaa tatgtccact tttgttgatt acaaaatcaa cctgattgat aagagcgatt
     2521 tcacgaaagc atctggcgtt gctaccgatg atatcgttgc tgtaggattg gtttaccagt
     2581 tctaatctga ttactcaaag atatgttgtg ggaggctttg cctccccaac atataagtgg
     2641 cgctctcaag ccactttcct tagaagcact atcttgcttc tgactatata aattaccctt
     2701 tattttctgg gacgtttcaa cgccccattt ttaataatac tcagtgaaca attaacgtat
     2761 caattagatt taataacaac gatatccgta cctaacctga tatctaatgt cataagcatc
     2821 cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca catagcccaa
     2881 acgccggatg caaaaaaaat catacgtgca tctctgaaat tgtgatggag gaagtgagta
     2941 tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga ttgttcaggc
     3001 tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc tgttgctcct
     3061 taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt atattaaaac
     3121 gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc ctggcaatct
     3181 cgctgatggg ttttactgtc gtttgtatag caggacaacg cattatttaa cctcacgtcg
     3241 tccaatatac tttgaaaact tattccctcg cttgccagac ggcgatgcaa tgtggaaaca
     3301 gaaatgtaga gatatctcgc taccttgttt gctgtccatt ttgttctagg ttcggatagc
     3361 attaggttat aacaacgact tattaatgat tgtttactat ctgataaaag taaacaatta
     3421 acatgatgtg ctcctaatga aagtagaacg cccattgcta agtgttcctg aatttgcgtt
     3481 gagaagcctc gggaaataga tgtttttagt tgctcccaac aatatattaa ctcggggttc
     3541 tgaggtaagc agaaacctgt cgtgttacga atttgatcag ttatcgcata aaatttttga
     3601 aacttttcaa ttagatcaat gggataataa agcatttctg caagataaag acctgcttct
     3661 ggataattca caatataaaa ttcatatcca cagggaaata atattatctg attattatca
     3721 acagttagac tatgcgtctc ccaattgata actttttttc cctgacggat acgacataaa
     3781 gctggcataa gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg tatttcttca
     3841 atctttaggt tagacttatc tcttgccagc atactcttac cctattttat ctcataaact
     3901 ggtgttatct cagcggttgc aattttattg gcattaagca tataaccaac taacgctccg
     3961 ctggaattag aatccacagg aatcttatca gtttttagag cccatacttt aaactggtaa
     4021 tgatggggtt tatctccttt gggaggacat gctccaccaa atcctgcata accaaaatca
     4081 tttcggcctt gaacagcacc agtcggaagt tttgtaccat cacgtcttcc tgcatcagtg
     4141 ggcaaatatg ttacagttgc tggaatatta gcaacagtcc agtgccacca tccactgcct
     4201 gtaggagcat ctggatcata cacagttacg gcaaagcttt tggtgccttc aggagcacca
     4261 gaccaggtta atgagggcga tgtattacca ccttcacacc caaatccaga aaatacatga
     4321 gacgttgtaa gttgctctcc tgtttttatt tcattactag tgacctgaaa tgctgcggcc
     4381 tgcgcagaaa atgttatgaa tgccaataca cttgaaacga taagtttttt cataaaaacc
     4441 tcattgttgt gacctatcgt tattttattt gatgtgcctt gatctcatta tgcataaagg
     4501 cgcaatgatc gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt agccataata
     4561 tgcacaaatc agattcctgt tatctcaagg agaattattt tttaatgcgg tgcacttcgt
     4621 gacaacaagt taatgactaa cacaccggca caaatcaaca tcatgcctat gatagctggc
     4681 aggtccagtc gttgaccaaa aaatccccag gaaagcaaac taatcagaac gataccgact
     4741 cctgaccaga tagcataagc aatccctgta ggtatataag ccagcgtctg agctaataac
     4801 cagaatgatg cacaatagca aataattgtg ccaacagagg gccataaccg tgtaaacccc
     4861 tctgaaaact tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc accaagataa
     4921 atataagggt tcatagcata ttctttcctg ttcatactgg agagaattgt actacagttt
     4981 gaacacaact caccgttttc atcctggtgt atgtgtatcc aaggggctct ggcgtggcaa
     5041 gagataaagg caggaacaat aatgatcaga aggaacccca ggctaacgaa tgagttcact
     5101 cttcctggga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg tggatggcag
     5161 aaaacaacca ggatcaggta tccattttaa accagaaatt gagtgagttt gccccatcca
     5221 tgccccacgc tgtgggatcg gatataatta aacaagcata gtcaacaggt tacacaacct
     5281 acacctgcat ggacatcact tcctgatacg ccgccaccag cttattacgc acctgaatcc
     5341 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt agcgccacgc
     5401 cgggttcacc gagggtgaat ttttctgcct gcgtgcgggc gactgtttgt gtatcgctta
     5461 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc ggcagtgatt
     5521 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     5581 gtatcgctga cattttcgtc tcccggataa tttctggtaa caaagcctac cagtaagtca
     5641 ataagacaaa ggcgctaaat agcaacaaaa aaactggttt attggcggat agaaaaaacg
     5701 aaagcacaaa taatgggagc gtcaattttt cgagtgtgct gacccgagag tgagtcttgt
     5761 tccactttgc caataacgcc gtccataatc agccacgaag tgcgcgatga atgcgactgc
     5821 agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga aaattccatt
     5881 gattgttacc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt gggcgaaagc
     5941 ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag
     6001 ccaactgacg caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaggt
     6061 tccggcagat aaagttcacg aactgcgcct ggcgcaacaa ggtttgccca aaggcggcgc
     6121 ggtcggtttc gaactgctcg atcaggaaaa gtttggtatc agccagttca gcgaacaggt
     6181 gaattatcag cgggcgctgg aaggcgagct ttctcgtacc atcgaaacta tcggcccggt
     6241 aaaaggggcg cgcgtacatc tggcaatgcc gaaaccgtct ttattcgtcc gtgaacaaaa
     6301 atccccttct gcatcggtga cggtaaatct gttacccggc cgcgcactcg atgaagggca
     6361 aattagcgct attgtacatc tggtttccag cgcggttgct ggtctgccgc ctggaaacgt
     6421 cacgctggtg gatcagggcg ggcatctgtt aacccagtcc aataccagcg ggcgcgatct
     6481 taatgacgct cagttgaaat atgccagcga tgtcgaaggc cgtattcagc agcgtattga
     6541 agcgatcctg tcacctattg ttggtaacgg taatattcac gcctaggtca cggcgcaact
     6601 ggacttcgcc agtaaagaac aaacggaaga acagtatcgc cctaacggtg atgaatctca
     6661 tgcggcgctt cgttcacgcc agcttaatga gagcgagcaa agcggttccg gttatccggg
     6721 cggcgtaccg ggggcgttgt cgaatcaacc ggcacctgcg aataacgcgc caatcagcac
     6781 gcctccgaca aatcaaaata accgccagca gcaggcgagc accaccagca atagtgggcc
     6841 gcgtagcaca cagcggaatg aaaccagtaa ctacgaagtc gatcgcacca ttcgtcatac
     6901 caaaatgaac gtgggcgatg tgcaacgtct gtcagtcgcg gtagtggtga attacaaaac
     6961 cttgccagac ggtaaaccat tgcctctcag caacgaacag atgaagcaaa ttgaagctct
     7021 gacccgcgag gcgatgggct tttctgaaaa acgcggtgat tcgctcaatg ttgttaactc
     7081 gccgttcaat agcagtgacg aaagcggcgg agcactgccg ttttggcaac agcaagtgtt
     7141 tatcgatcag ttgctggctg ccggtcgctg gttgctggtt gctggtactg ctggtggcgt
     7201 ggttgctgtg gcggaaagcg gtacgcccgc agctaacacg tcgcgccgag gcggtgaaga
     7261 ctgtgcagca acaggcgcag gcccgcgagg aagtggaaga tgcggtggaa gtccgtctga
     7321 gcaaagacga acaactccaa caacggcgcg ctaaccaacg actgggggca gaagtcatga
     7381 gccagcgtat ccgtgaaatg tctgataacg atccgcgcgt ggtggcgctg gtcattcgcc
     7441 agtggataaa taacgatcat gagtaacctg acaggcaccg ataaaagcgt catcctgctg
     7501 atgaccattg gcgaagaccg ggcggcagag gtgttcaagc acctctccca gcgcgaagtg
     7561 caaaccctga gcgctgcaat ggcgaacgtc acgcagatct ccaacaagca gctaaccgat
     7621 gtgctggcgg agtttgagca agaagctgaa cagtttgctg cactgaatat caacgccaac
     7681 gattatctgc gttcggtatt ggtcaaagct ctgggtgaag aacgtgccgc cagcctgctg
     7741 gaagatattc tcgaaacccg cgataccgcc agcggtattg aaacgctcaa ctttatggag
     7801 ccgcagagcg ccgccgatct gattcgcgat gagcatccgc aaattatcgc caccattctg
     7861 gtgcatctga ggcgcgccca ggccgccgat attctggcgt tgttcgatga acgtctgcgc
     7921 cacgacgtga tgttgcgtat cgccaccttt ggcggcgtgc agccagccgc gctggccgaa
     7981 ctgaccgaag tactgaatgg cttgctcgac ggtcagaatc tcaagcgcag caaaatgggc
     8041 ggcgtgagaa cggcagccga aattatcaac ctgatgaaaa cgcagcagga agaagccgtt
     8101 attaccgccg tgcgtgaatt cgacggcgag ctggcgcaga aaatcatcga cgagatgttc
     8161 ctgttcgaga atctggtgga tgtcgatgat cgcagcattc agcgtctgtt gcaggaagtg
     8221 gattccgaat cgctgctgat cgcgctgaaa ggggccgagc agccactgcg cgagaaattc
     8281 ctgcgcaata tgtcgcagcg tgccgccgat attctgcgcg acgatctcgc caagcgtggt
     8341 ccggtgcgtc tgtcgcaggt cgaaaacgaa cagaaagcga ttctgctgat tgtgcgccgt
     8401 cttgccgaaa ctggcgagat ggtgattggc agcggcgagg atacctatgt ctgataatct
     8461 gccgtggaaa acctggacgc cggacgatct cgcgccacca ccggcagagt ttgtgccaat
     8521 ggtcgagtcg gaagaaacca ttattgaaga ggccgaaccc agccttgagc agcaactggc
     8581 gcaactgcaa atgcaggccc acgagcaggg ttatcaggcg gggattgccg aaggtcgcca
     8641 gcaaggtcac gaacagggct atcaggaagg actggcccag gggctggaac aaggtctggc
     8701 agaggcgaag gctcaacaag cgccaattca tgcccggatg cagcaactgg tcagcgaatt
     8761 tcaaactacc cttgatgcgc ttgatagtgt gatagcttcg cgcctgatgc agatggcgct
     8821 ggaggcagca cgtcaggtga tcggtcagac accaacggtg gataactcgg cactgatcaa
     8881 acagatccaa cagttgttgc agcaagaacc gttattcagc gggaaaccac agctgcgcgt
     8941 acacccggat gatctgcaac gtgtggatga tatgctcggc gcaactttaa gtttgcatgg
     9001 ctggcgcttg cggggcgatc ccaccctcca ccctggcgga tgtaaagtct ccgccgatga
     9061 aggcgatctc gacgccagtg tcgccactcg ctggcaagaa ctctgccgtc tggcagcacc
     9121 aggagtggtg taatgaccac gcgcctgact cgctggctaa ccacgctgga taactttgaa
     9181 gccaaaatgg cgcagttgcc tgcggtacgt cgctacgggc gattaacccg cgctaccggg
     9241 ctggtgctgg aagccaccgg attacaattg ccgctcggcg caacctgtgt cattgagcgc
     9301 cagaacggca gcgaaacgca cgaagtagaa agcgaagtcg ttggctttaa cggtcaacgg
     9361 ctgtttttaa tgccgctgga ggaagtggaa ggtgtcctgc ccggcgcgcg tgtatatgcc
     9421 aaaaacattt cggcagaagg gctgcaaagc ggcaagcagt tgccgctcgg tccggcgtta
     9481 ttaggtcgtg ttctggacgg tagcggtaaa ccgctcgatg gcctgccctt ccccgatacg
     9541 acggaaaccg gtgcgctgat taccccgcca tttaacccgt tgcaacgtac accgattgaa
     9601 catgtgctgg acaccggcgt gcgcccaatc aatgccctgc ttaccgtcgg acgcgggcag
     9661 cgtatggggc tgtttgccgg gtcgggcgtt ggtaaaagtg tgctgctggg gatgatggca
     9721 cgctacaccc gcgccgatgt cattgtcgtg ggtttgattg gtgaacgtgg gcgcgaagta
     9781 aaagatttta ttgagaatat cctcggtgcc gaaggacgtg cacgctcagt ggtgattgcc
     9841 gctccggcgg atgtttctcc gctcctgcga atgcaaggtg ccgcctatgc cacgcgcatt
     9901 gccgaagatt ttcgcgatcg tggtcagcat gtgttgctga ttatggactc cctcacccgc
     9961 tacgcgatgg cccagcgtga aattgcgctg gcgattggcg aaccacctgc cactaaaggt
    10021 tatccaccgt cggtgtttgc caaattaccg gcactggtcg agcgtgccgg taatggcatt
    10081 agcggcggcg gctcgattac cgcgttttat accgtgctca ccgaaggcga tgaccagcag
    10141 gaccccattg ctgactccgc gcgggcaatc ctcgacggtc acattgtgct gtctcgccga
    10201 ctggcggaag ccgggcacta tccggctatc gatattgaag cgtcgatcag tcgcgcaatg
    10261 acggcgttga tcagtgagca acattacgcg cgagtgcgca ccttcaaaca gctgttgtcg
    10321 agttttcagc gtaaccgcga tctggttagc gtcggcgcgt atgccaaagg cagcgatccg
    10381 atgctcgata aagccatcgc cctgtggccg cagctggagg gctatttgca acaaggcatt
    10441 tttgaacgcg cggactggga agcgtctctc caggggctgg agcgtatttt cccgacagtg
    10501 tcataaccca ggagataacg gcagatggcg gaagaacagc tcaaaatgtt gatcgattat
    10561 cagaatgaat atcgcaataa cctcaatagc gatatgagtg ccgggatgac cagcaaccgc
    10621 tggatcaact atcagcagtt tatccagacg ctggaaaaag ccattactca gcatcgccag
    10681 caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg agaaaagaaa
    10741 caacgtttgc aggcctggca gacactgcag gaacggcaat ccacggcggc actgcttgca
    10801 gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc catgaggaaa
    10861 cctgaatgat tcgcttagcg cccttgatta ccgccgacgt tgacaccacc acattgcctg
    10921 gcggcaaagc cagcgatgct gcacaagatt ttctcacgtt gttgagcgaa gcattagcag
    10981 gcgagacaac aactgacaaa gcggcccccc agttgctggt agcaacagat aagcccacga
    11041 caaaaggcga gccgctggtc agcgatattc ttgccgatgc gcaacaagcg gatttactga
    11101 tccctgtgga tgaaacactt cctgtcatca acgacgaaca atccacatca acaccgttaa
    11161 ccaccgctca gacgatgacg ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa
    11221 aagcggatga tcttaatgaa gacgtcaccg caagtctgag cgcccttttt gcgatgttgc
    11281 cgggttttga caatacgccc aaagtgactg atgcaccgtc aaccgtgtta ccggcagaga
    11341 aaccaacgct cttcacaaaa ctgacttctg cgcaactcac tacagcacag cctgatgacg
    11401 cccccggcac accagctcag ccattaacac cgctggtagc agaagcccag agtaaagcgg
    11461 aagtcatcag cacgccttcg ccggtgaccg ctgacgccag cccgctaatc actccacacc
    11521 agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg ggttctcacg
    11581 aatggcaaca atcattgagc cagcatattt cgctgttcac ccgccagggg caacaaagtg
    11641 cagagttgcg tctgcaccca caggatttag gtgaagtgca aatttccctc aaagtggatg
    11701 ataaccaggc gcagatccag atgatttcac cgcatcagca cgtacgcgcc gccctggaag
    11761 cagcgctgcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta gggcaaagca
    11821 acattagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccagcaa cagcaaagcc
    11881 aacgcacagc aaaccatgaa cctctggcgg gggaagacga cgatacgctt ccggttcccg
    11941 tctctttaca agggcgtgta acaggtaaca gcggcgttga tattttcgcc taacgtcaga
    12001 ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaagacgc aggataatta
    12061 gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg cgataagcaa
    12121 gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg cggcctgtgc
    12181 cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca aagcgcagca
    12241 acgcgtcgtg ccctccccgg tcttctatgc gctggatacc tttacggtca atttgggcga
    12301 tgcggatcgc gtgctttata tcggcataac cctgcgactg aaagatgaag caacccgctc
    12361 gcggctgagt gagtatttgc cggaagtccg tagtcgcttg ctgttactgt tttcacgtca
    12421 ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg agattaaaac
    12481 cacactttcc accccgcttg ttgccgggca accgaaacag gatgtcaccg acgtgctgta
    12541 taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa gctgaaattg
    12601 atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt attagcggcg
    12661 aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc
    12721 aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg ctgttcaacc
    12781 tgctgcgccg tagcccggac attaccgtcg gggccatccg cattcagccg taccatgaat
    12841 ttgcccgcaa cctgccggtg ccgaccaacc tgaacctaat ccacctgaaa ccgctacgcg
    12901 gcacagggct ggtagtgttc tcgccgagtc tggtgtttat cgccgtggat aacctgtttg
    12961 gcggcgatgg acgcttcccg accaaagtgg aaggtcgcga gtttacccat actgaacagc
    13021 gcgtcatcaa ccgtatgttg aagctggcgc tggaaggcta tagcgacgcc tggaaagcga
    13081 ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtggaattt accaatatca
    13141 ccacctcgcc gaacgacatt gtggttaaca cgccgttcca tgtggagatt ggcaacctga
    13201 ccggcgaatt taatatctgc ctgccattca gcatgatcga gccgctacgg gaattgttgg
    13261 ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc
    13321 gccaggtgca gcattcacag ctggaactgg tggccaactt tgccgatatc tcgctacgcc
    13381 tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa cccgatcgca
    13441 tcatcgccca tgttgacggc gtcccggttc tgaccagtca gtacggtact ctcaacggtc
    13501 agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg aacgaggaac
    13561 agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa tggacgatct
    13621 gtgggctgaa gcgttgagcg agcaaaaatc aaccagcgaa aaaagcgctg ccgacgcggt
    13681 gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg acctgattat
    13741 ggatattccg gtcaagctga ccgtcgagct gggccgtacg cggatgacca tcaaagagct
    13801 gttgcgtctg acgcaagggt cagtcgtggc gctggacggt ctggcgggcg aaccgctgga
    13861 tattctgatc aacggttatt taatcgccca gggcgaagtg gtggttgttg ccgataaata
    13921 tggcgtgcgg atcaccgata tcattacccc gtccgagcgg atgcgccgcc tgagccgtta
    13981 gtgatgaata accatgctac tgtgcaatct tccgcgccgg tttctgctga gccactgctg
    14041 caggtgagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc ctggctggta
    14101 aaacggctgg gatttgcccc taaacgcact ggcgttaacg gtctgaaaat tagcgccagt
    14161 gcttcgctgg gcgcgcgtga acgggtcgtg gtggtcgatg tggaagatgc acggctggtg
    14221 ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc agcaccaacg
    14281 gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa gcgtggcggg
    14341 agatcctgat gcgtcgtttg ttttctgtcg caccggtcct tctctggctg attacgcccc
    14401 tcgccttcgc gcaactgccg ggtatcacca gccagccgct acctggcggt ggacaaagct
    14461 ggtcgctccc ggtgcagacg ctggtgttca tcacctcgtt gacgttcatt ccagcgattt
    14521 tactgatgat gaccagtttc acccgcatca ttattgtttt tggtttattg cgtaacgcgc
    14581 tgggaacgcc ctccgcgcca cctaaccagg tattgctggg gctggcactg tttttgacct
    14641 tttttattat gtcgccagtg atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg
    14701 aagagaaaat ctcaatgcag gaggcgctgg aaaaaggggc gcagccgctg cgtgagttta
    14761 tgctgcgtca gacccgcgag gcagatttag ggttgtttgc cagactggcg aataccggcc
    14821 cgttgcaggg gcctgaagct gtgccgatgc gcattttgct cccggcctac gtgaccagcg
    14881 agttgaaaac cgcatttcag ataggcttca cgattttcat cccttttttg attatcgacc
    14941 tggtgattgc cagcgtgttg atggcattgg ggatgatgat ggttcccccc gccaccattg
    15001 ctctgccctt taaactgatg ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt
    15061 cgctggcgca gagcttttac agctagagag gcaaaatgac acctgaatcg gtcatgatga
    15121 tgggaactga agcgatgaaa gtcgcgctgg cactggcagc cccgctgttg ctggtagcgc
    15181 tggtcacggg ccttatcatc agtattttgc aggcagccac gcagattaac gaaatgacgc
    15241 tgtcgtttat tccgaaaatc atcgccgtat ttatcgccat cattattgcc ggaccgtgga
    15301 tgctcaatct gttgctggat tacgtccgca ccttgttcac taacctgccg tatatcatcg
    15361 ggtagcccgt acaatgatgc aggagacaag cgatcaatgg ctttcctggt taagcctgta
    15421 cttctggccg ttactgcgcg tgctggcgct catttctacc gcgccaattc tgagcgaacg
    15481 cagcgtgccg aaacgggtaa aactgggtct ggcaatgatg atcacgttcg ccattgcccc
    15541 atcattacct gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc tggccgtaca
    15601 gcagatcctg atcggcattg cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg
    15661 aaccgctggc gaaattatcg gtctgcaaat ggggctgtca tttgcgacat ttgtcgatcc
    15721 tggcagccat ctcaatatgc ccgttttagc gcgcatcatg gatatgctgg cattactgct
    15781 gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata cctttcacac
    15841 cctgccgatt ggtagcgaac cgttgaacag caatgcgttt ctggcaccca ccaaagcagg
    15901 gagtttgatt ttccttaacg ggctgatgct ggcgttaccg ctcattactc tgttgctgac
    15961 cctgaatctg gcattaggtt tactgaatcg tatggccccg caattatcca tttttgttat
    16021 tggatttcca ttaactctga ctgtcggcat ctctttaatg gcggcattaa tgccgttaat
    16081 tgcgcctttt tgcgaacatt tattcagtga aatttttaat ttgctggctg atattattag
    16141 tgaattacca ttaatataat tccgtaacgt ttatcatgct accctaagga ttatccgaaa
    16201 aataatatct gcgaacatct tccaggatac tcctgtagcg aaatatttgt tttaagctca
    16261 ctcacatatc gcaacattta ctttaagaca attccaggca aattatacaa cactttacgg
    16321 gatagtaagt ccgcctgaaa aattgcgaga gtggcgcatt aggtgaccca tgttgttccg
    16381 tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga gtgagggtat
    16441 gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat
    16501 ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt
    16561 gccatagctt gtgattcaca gcgtccttca gtggtgttta ttaatgagga ctgtttcatc
    16621 cacgatgctt ctaacagtca gcgcatcaag cacatcatta atcaacatcc caatacgtta
    16681 tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa
    16741 aatttattga tcagttctaa atcgattaaa ccggaatctc tcgacgatat ccttggcgat
    16801 attctgaaaa aagagacaac gataacctcg tttttaaata tgccgacgtt atcattgagc
    16861 cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat tcaaatctcc
    16921 gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat taaacgtaag
    16981 atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga taatgtgact
    17041 aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc accagtcagg
    17101 ctgaataaga ttactctgct ttctcaacaa agataccgtc ctgatgccct gcttcattaa
    17161 agaaccagat gccgagcggg tagtcttcca gcgcaaccag gtacattgtg ccttcactaa
    17221 actcctcaac tgccagtacc actccaggac gacgcggacc gccatccgtt ttgactgtta
    17281 cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta gaacaatttc
    17341 gtttttctgg cagcgccaag cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag
    17401 agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc gaggaaagat
    17461 gatgaaaacc gcaaaagtgt acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga
    17521 tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca gccagaacga
    17581 ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat tggcggatgc
    17641 cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa
    17701 aaaaatcccg accctgaggg tgtcgggatg aaacttgctt aagcaagaag cacttaaaaa
    17761 attcgttaca ccaggaaatc tgatgtgttc atcaccttat ctgcaatttt tttcgctgac
    17821 aagaaaatat tcgcgctatg aatgattaac gctacttatg aatagaaatg tgacgcgcgt
    17881 caccccttgg tggctctggt gtcaacggga tcgctcccga aaaaatcatc tttgattatt
    17941 tgtgctgttt tgagaatcat cctggtaagg ggagtactgc gcgaccatgt tttcaattca
    18001 acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc atagttatga
    18061 ctggcaaccg gcagccccct ggctcagccg tttacacgaa gccaatattc ccgtcattct
    18121 ctgtagcagt aaaactttag cggaaatgct gtacttgcaa aaaatgttgg ggctacaagg
    18181 cttaccgctg attgcagaga atggcgcagt gatccagctt gctgaacaat ggcaggatat
    18241 agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatctgcc aggttttaaa
    18301 tacgctacgc gagaaagaac attttaaatt cacgactttt gatgatgtcg acgatgcaac
    18361 cattgccgaa tggacgggat taagccgtag ctaggcggcg ctgacgcagc tgcatgaggc
    18421 gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta tcgctcgtct
    18481 gaacgaactg ggcttgcagt ttatgcaggg tgcgcgcttc tggcacgtac tggatgcctc
    18541 tgccggaaaa gatcaggctg ccaactggat tatcgcgacc tatcaacaat tgtcaggcag
    18601 acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcctttac tggaggtaat
    18661 ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gtacatctgc atgatgagga
    18721 tccggcccgc gtctggcgaa cgcagcgtga aggaccggaa ggctggcgtg aagggctgga
    18781 ccattttttc tccgcccgtt aagcgttatc gctcgcgaat acccgattgc gcccggcctg
    18841 ttttgcgagg taaagccgac ggtcggccaa tgactggagt tgttcaaaat cataatcacc
    18901 ggtttcctcg ctgctactta cccccagcga ggcactgatg cgtatcgttg tactcttggc
    18961 gatcagcatc tctttttcat ttaacttcag gcgaatacgt tctgcgacct ccgcagcctg
    19021 cgtcagattc gcgcctggca gaatcacaca aaactcctca ccaccgaccc gcccggcaac
    19081 gtcctgcgca cgcaaggaac tgctaattaa tccggcagca tgagaaagaa cacggtcgcc
    19141 cgcctgatga ccaaagcggt cattaatcgc tttaaaatgg tcaagatcga cctgaatgac
    19201 agaaaaagga tgttggtgcg tctgacacaa tttagcgagc ggacgggctt tttcgaacag
    19261 tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac gcctgccact gcaacgagct
    19321 ttgcagaaca tacatgttgc tgaccatccg gcgaatcaca taccaggaga ggagtaacat
    19381 ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga aatcgccgcg
    19441 cacgccttcg cttagcgtat ggacgcgcgc cagcacaccg tcgaaatggt ccagacgttc
    19501 ccagctaaca tagcgactgt ccatacgaat gccgccccgc gtgtcatgtt ccattgcctg
    19561 cgccagcaag gccagttcac gaggatcaaa aatattccct gttggatggt caggattgga
    19621 agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga ggtttttatc
    19681 gatggcgttt cttaaaaatt gctgcatagt acgcacggga atactcatcc ccagcacgcc
    19741 ataccagtag ttattactgt ctaccggaac actgacggta acctgcggtt cagtattgct
    19801 ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt ctcgttgcga
    19861 atggccgata aaccagggtt gggtgacata gccgtaataa cgcgttggta cattgcgcgt
    19921 aaacaaggtc ggctgcgtcg aaacgtaaaa tccagcacgc gagacataca tcgcctgttc
    19981 aaccatcgac gaggagttgt gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt
    20041 aatttcattg tcgaggcttt cattttcgcg agacaggaaa ttcccctcgc tgactaatgc
    20101 atccgatacg ccattgactg gcagggtgcg ccgtcggttg agttcgattt gccaggcgtg
    20161 ctcgtcgcga tgctgttcga actcggttac ggcattacgc agagaggtga aatccagtgg
    20221 cgccacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga cgttatactg
    20281 caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctagc tggagatata
    20341 ggcatcttcc agcaccacca cttcacgcca ggtgagcaag gtggaaaaaa gcaataccac
    20401 gataaagcag agattaacga catgaccggg ccccaggcgg cgtgcgagtt ttttcaacca
    20461 gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc tgctttttta
    20521 tgattctggc ataacccggc aatagcgtac cgcaaaacag cgataatttt ataatggtgg
    20581 catgataaga cgcgactatc attcattgca ggcattggga caccaggccg gatgtgatcc
    20641 ggcctggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc ctcccccgga
    20701 acaagatcgg tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata ccaacgacac
    20761 tcaaccatac cgctggagta tccagtgaca atcatccgcg ggccgccctc tttaaccgta
    20821 acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa acttgttcac
    20881 cttagttgaa gatggcgaat tttgcataat agccagtgcg agtattaatg tgcctgaccg
    20941 cgcattttcg ctactgcttt cactcccagc accacgatgc cgccgatgat aaatccaaga
    21001 atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg cccggcgata
    21061 tgttcaatag cgtgatgcag cggcgcaata ccatgtacca caatcccgcc gccgacgagg
    21121 aacatcgcca gcgtgccgac aatcgataat gctttcatca gccaaggcgc gataatcaat
    21181 aaccctttac ctaatgcctg catcagcgcg ctagattttt ccgccagcca ataccctagg
    21241 tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac taccagcgcg
    21301 atgcctgaaa gcaccagcac ctgattaagc aacggcgcct ctgccacaat ccccagcgtg
    21361 atggcgacga tttccgcaga caaaataaaa tcggtacgaa tcgccccttt aattttgtcc
    21421 ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg gctctgcgct
    21481 ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac cccttcaaag
    21541 cagagaaacg cgccaccaat catcaacagt ggcgtaatcg cccacgggat aaacgcactg
    21601 atgatcagcg ccagcggcac cagaatcact ttattgatca gcgatccttt cgccacgccc
    21661 cagaccacgg gaagttcccg gttggcccgc acgcctgaaa cttgttgcgc attgagcgat
    21721 aaatcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat cacagagata
    21781 tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc ggccaaaagc
    21841 attccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat
    21901 acttgcttca caggtcaaca tttatttaac aatcaagtgc aattatatct ctcgccagca
    21961 gaatgatttt cacgtttact atgaaccacc tttttatttc cgccgtgagg gattatgcgt
    22021 ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca
    22081 acctattttg tcattcggat tggcgtggga agctggcctc cgttaatgat ggcgggcgtt
    22141 cgattccagg cagccggtat tttattgctg gcatttttgc tactgcgcgg acacaaactt
    22201 cccccgctac gtccgctgct caatgccgcg ctgattggcc tgttattgct ggctgtcggt
    22261 aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc cgccgtagtg
    22321 attgcaaccg tgcccctctt taccctgtgc ttcagtcgcc tgtttggcat taaaacgcgc
    22381 aaactggaat gggtgggtat tgccattggg cttgccggaa tcatcatgct caatagcggt
    22441 ggaaatttaa gcggcaatcc gtggggcgcg attctgattt taatcggctc gattagctgg
    22501 gcgtttggct cagtttatgg ctcgcgcatt accttacctg tagggatgat ggcgggtgcg
    22561 attgagatgc tggcggcagg cgtggtgtta atgatcgcat cgatgattgc gggtgaaaaa
    22621 ctgacggcgc tcccttccct gtcaggcttc cttgcggtcg gctatctggc gctgtttggt
    22681 tcgattatcg ccattaacgc ttatatgtat ttaatccgta atgtcagtcc ggctctcgcc
    22741 accagctacg cctacgttaa cccggtggtc gcggtcttgc tgggtacggg actgggtggc
    22801 gaaacgctgt cgaagattga atggctggcg ctcggcgtaa ttgttttcgc ggtggtactg
    22861 gtcacgttgg gaaaatatct cttcccggcc aaacccgttg tcgcgccagt tattcaggat
    22921 gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac cttcgccgca
    22981 gatccactct tccagacgct cggtaagcgc cgcatccgtc agcttctcgc gcctacgtaa
    23041 cgcacactcc cagacaatca gtacgcgcca gccgagctcc tgcaagcgac tgatatcgcg
    23101 gcgatcgcgt tcaacatttt tacctatctt ctccagccag aactcagttc gagtcgcagg
    23161 cactttaaac agatagcagt gatgatgatg ccagaagcag ccgtgggtaa atatcacgca
    23221 gcgatattca tcaacgacaa aatccgggcg tccgggcaga ctggcgtcct gaacgcgaaa
    23281 cgcaaggccc tgcccggtta acaaactggc gaggcgcttc tcaatcgccg tatcacgcgt
    23341 ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga ccatgttgtg
    23401 cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt tttgccaccg
    23461 cggcaaagac cggcacgacc accgagttgc cgaactggcg ataggcctga gtgtccgaaa
    23521 ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc cattaagcgc gcgcattcac
    23581 gaggcgttaa ccgacgtgga cgatgttgct gattcagcgg atcgtcaaag tctttctcac
    23641 ccgtggccat atcccaaccg cgatcgatta aaatttccgc gccatctttg taataacgag
    23701 cagacagcgt acgggtgacg ctttgcggat tgttcggata aaccattcca taaccgaagc
    23761 cgttaccgcg cgcctgatgt tttttcgcat atcgatagag gtacttccac agcaccggcg
    23821 tcaggatata tttcgcctca accatcgggt ccagcagctg cgccagcgtc actcgctgtg
    23881 cagggaaaca ttcgctgata tcacgcagag taaaatcggc tttcagattc agatcgcgac
    23941 gaaaacccac cagcacgatg cgttcacggt gctgcggcag gaaatgttta ccatcgatga
    24001 ttttcggatc gtccggcccg ttatcttctg catcggcaac gtcatagccc agttcgtcca
    24061 gcgtctgcat gatgatgcgg aacgtttttc ccttatcgtg actcttcagg tttttgacgt
    24121 tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca tcaaagaaca
    24181 gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc tttttcgata
    24241 cgccagccag cgaaaatggc tgacaaggga aaccggccag taaaacatcg tgttcaggaa
    24301 tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttga tgactgaggg
    24361 tgatgtcgcg gatatcttca ttaaaatgat gcgtcgccgg gtcgcaataa tggttggctt
    24421 tataagtgcg taccgcatgt ttgttccatt cgctggtaaa cacgcactgt ccaccaatcg
    24481 attcaaaacc acgacggatg ccgccaattc cggcaaatag atcgataaag cgaaacgcat
    24541 aatgcggatg atgttccggt ggtttgggta acaacgtttg cagatgggca aactctttct
    24601 cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt gccgcgctcc
    24661 agtgattctc acccacacca ttaagctgcg ccaccagcat tttaacatca taaatttgca
    24721 gcagtttctc cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg gttactgata
    24781 tattttcctg catagattta accggccatc tgagatggcc gacaggttaa cataattact
    24841 gccgtttagg gccacgcgct cagccagcgg gtgaaaacgc atctatcacc ttgtgatcga
    24901 taccttcatt ctcccccgcc agttcggcac cgagcttcgc catcaaacag cgccaagctc
    24961 aatgcccctg aaacggcaaa tacgcgcgcc aggccaatcg cccccagggc ttccagccga
    25021 tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc gctgaaactg
    25081 tgagcggcaa tggcatgaca aacggcctca attttctccg ccggaaattg cacgaactct
    25141 tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg ccgttgcgga
    25201 tgatttttcg ccaggctcac gatatcgtga aaataacagg cggttaaaat caccagcata
    25261 tcgacatcgt catctgccgc cagtttctgt gccgtagccc agacacggcg aaaatgacac
    25321 aggtcatgcg ccgcgtcctg atgttgatga tggtttttca accagttctc gaactatgcc
    25381 tgccagtgtt gtaactccat acgcgctccg ttgccaaaga gtctcaacct tagcagtttt
    25441 tattcatagc gtctgcaacg tgcgggacga ggtcgggtgt accatgcaat gccgcctaac
    25501 aaggcaccaa ccagggccat aaacagaaat ccgaccaatg ctcctaacca cttccccgcg
    25561 gtagatccta aatcactttc aaatcccgac acgggggcat cggggatccg gctcatcacc
    25621 cagatgtatc gcgccattgc ccagacgaaa tagccacacc atgtatagaa agcccac
//
LOCUS       DAJTYH010000059        25133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTYH010000059 DAJTYH010000000
VERSION     DAJTYH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..991)
                     /gene="yeaG"
                     /locus_tag="OO609_002359"
     CDS             complement(<1..991)
                     /gene="yeaG"
                     /locus_tag="OO609_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6633746.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVP"
     gene            1427..2173
                     /gene="mipA"
                     /locus_tag="OO609_002360"
     CDS             1427..2173
                     /gene="mipA"
                     /locus_tag="OO609_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6633747.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2263..3024
                     /locus_tag="OO609_002361"
     CDS             2263..3024
                     /locus_tag="OO609_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6633748.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3075..3959)
                     /locus_tag="OO609_002362"
     CDS             complement(3075..3959)
                     /locus_tag="OO609_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6633749.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4043..5038)
                     /gene="gapA"
                     /locus_tag="OO609_002363"
     CDS             complement(4043..5038)
                     /gene="gapA"
                     /locus_tag="OO609_002363"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6633750.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5380..5793
                     /gene="msrB"
                     /locus_tag="OO609_002364"
     CDS             5380..5793
                     /gene="msrB"
                     /locus_tag="OO609_002364"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6633751.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5835..6107
                     /locus_tag="OO609_002365"
     CDS             5835..6107
                     /locus_tag="OO609_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6633752.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6477..7553
                     /locus_tag="OO609_002366"
     CDS             6477..7553
                     /locus_tag="OO609_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6633753.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7580..8959
                     /locus_tag="OO609_002367"
     CDS             7580..8959
                     /locus_tag="OO609_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633754.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            8976..10021
                     /locus_tag="OO609_002368"
                     /pseudo
     CDS             8976..10021
                     /locus_tag="OO609_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10042..10878
                     /locus_tag="OO609_002369"
     CDS             10042..10878
                     /locus_tag="OO609_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6633755.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            10883..11830
                     /locus_tag="OO609_002370"
     CDS             10883..11830
                     /locus_tag="OO609_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6633756.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            11840..12820
                     /gene="ydjG"
                     /locus_tag="OO609_002371"
     CDS             11840..12820
                     /gene="ydjG"
                     /locus_tag="OO609_002371"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6633757.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            12957..13715
                     /locus_tag="OO609_002372"
     CDS             12957..13715
                     /locus_tag="OO609_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6633758.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            13833..15191
                     /locus_tag="OO609_002373"
     CDS             13833..15191
                     /locus_tag="OO609_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633759.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15284..15925)
                     /gene="pncA"
                     /locus_tag="OO609_002374"
     CDS             complement(15284..15925)
                     /gene="pncA"
                     /locus_tag="OO609_002374"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6633760.1"
                     /translation="MPHRTLLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(15936..16952)
                     /gene="ansA"
                     /locus_tag="OO609_002375"
     CDS             complement(15936..16952)
                     /gene="ansA"
                     /locus_tag="OO609_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6633761.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17119..18975)
                     /gene="sppA"
                     /locus_tag="OO609_002376"
     CDS             complement(17119..18975)
                     /gene="sppA"
                     /locus_tag="OO609_002376"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6633762.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19136..19687
                     /locus_tag="OO609_002377"
     CDS             19136..19687
                     /locus_tag="OO609_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6633763.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19804..20847
                     /gene="selD"
                     /locus_tag="OO609_002378"
     CDS             19804..20847
                     /gene="selD"
                     /locus_tag="OO609_002378"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6633764.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            20852..22813
                     /gene="topB"
                     /locus_tag="OO609_002379"
     CDS             20852..22813
                     /gene="topB"
                     /locus_tag="OO609_002379"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6633765.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            22940..23980
                     /locus_tag="OO609_002380"
     CDS             22940..23980
                     /locus_tag="OO609_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6633766.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24097..>25133)
                     /gene="gdhA"
                     /locus_tag="OO609_002381"
     CDS             complement(24097..>25133)
                     /gene="gdhA"
                     /locus_tag="OO609_002381"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6633767.1"
                     /translation="SILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFC
                     QTLMTELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSL
                     IRPEATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASD
                     SSGTVVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPC
                     ATQNELDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVAT
                     SGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKV
                     ADAMLAQGVI"
BASE COUNT         6388 a         5836 c         6489 g         6420 t
ORIGIN      
        1 acggcacctt cacgatgtaa acacggtcga ggaaggcttc gttgtttttg ttattacgga
       61 aagtgaccca ttcggacttg ttcgagtggg cgagaataat cccgttgaac ggcagggcgg
      121 agataccttc cgtcccgttg tagttacctt cctgagtggc ggttaacaag ggatgcagca
      181 cttttatcgg tgctttaaac atctcaacga attccatgat cccctgattg gcgcggcaca
      241 gcgcaccgga atagccgtag gcgtccgggt cattctgcgc gtagtgttcg agtttacgaa
      301 tatcgacttt cccaaccagc gcggagatgt cctggttgtt ctcatcaccg ggttccgttt
      361 tggcgatagc aatttgttgc agaattgacg gccagacctt cactacccgg aacttagtga
      421 tatcgccacc aaattcatgc aggcgttttg ccgcccatgg cgacatgatg gtgccgagat
      481 aacggcgagg aatgccatac tctttttcca gaatctgagc atcttcctgc ggattgaaaa
      541 gacagaacgg atgatcgttg accgggctac gctcaccgtt ggcgctcaat acataaatcg
      601 gtacgagctg cattaatgat ttcagtcgct cagcaagcga tgatttaccc ccacccacag
      661 gccccagcag atacaggatt tgtttcttct cttccagccc ctgagccgcg tgtttcagat
      721 aagagacaat ctgttcaatc gcgtcttcca tgccgtaaaa ctcttcaaat gccggataac
      781 gtgcaatgac ccggttagaa aagagtcgag aaagtctggg gtcctgggct gtatcgacca
      841 tgacaggctc accgatagcc atcaatagcc gctcagccgc gttggcataa gcactgcgat
      901 cttgccgaca agtggtaaga aactcctgca gtgtgaactc ttcgtccttg gcagcttcat
      961 atcgctggcg atagtgatcg aatatattca tggtatgccg tcctttcgtt ttttagcaca
     1021 ggttaagagc cgttcatatg aatagtagag gctcccggaa gaggtaaact gaacgacgtc
     1081 tcttgctaaa acacagcaac tctcatgcca ttttatcgac gatgagaaca acgtgcccgg
     1141 tgatacacct tcaaaattaa gcgtagatgg caaatggaaa acttgcctgc acggctttct
     1201 taatttcaat ggcatatcaa caactcatcc ataaattttg cataattaat gtaaagacca
     1261 ggctcgccag taacgctaaa tccatttggc tgtaagcgcg gtgtcatccg cggcaggaaa
     1321 attaaacagt tactttaaaa aatgaaaacg taaaaaggtt gggtttcgat gtattgacgg
     1381 gtaaactttg tcgcccgcta aacatttgtt taaggaatga ttaattgtga ccaaactcaa
     1441 acttctggca cttggagtgc ttatcgcaac gtctgcaggc gtagcgcacg ctgaaggtaa
     1501 attttccctg ggcgcaggcg taggtgtcgt tgagcaccca tataaagatt acgataccga
     1561 tgtttaccca gtaccggtaa tcaactatga aggcgataac ttctggttcc gtggcttagg
     1621 tggtggttac tacctgtgga atgacgcaac ggataaactt tcaattaccg cttactggtc
     1681 gccgctttac ttcaaagcga aagacagtgg cgatcaccaa atgcgtcacc tggatgaccg
     1741 taagagcacc atgatggctg gtctgtctta tgctcacttt acccagtacg gttacctgcg
     1801 taccaccctg gctggcgata ccctggataa cagcaacggc attgtctggg atatggcctg
     1861 gttgtatcgt tacaccaacg gtggcctgac cgtgactccg ggtattggtg tgcagtggaa
     1921 cagcgaaaac cagaacgaat actattatgg cgtatcgcgc aaagagtccg ctcgcagcgg
     1981 tctgcgtggc tataacccga atgacagctg gagcccttac cttgagctga gcgccagcta
     2041 caacttcctc ggcgactgga gtgtttacgg taccgcgcgc tacacccgtc tgtctgatga
     2101 agttactgac agcccgatgg tggataaatc ctggactggc ctgatttcta ccgggatcac
     2161 ctacaaattc tgataatgca tctttccagg gcattttgtg cataaaaaca gggcgtcacg
     2221 ccctgttttg cattacggtg gtgcaatacc tgggaggaaa caatgcaaca aaaaatgatt
     2281 caatttagtg gcgatgtctc actgccagcc atagggcagg gaacatggta tatgggcgaa
     2341 gatgccagtc agcgcaaaac agaagttgct gcactacgcg caggcattga actcggttta
     2401 accctcattg ataccgccga aatgtatgcc gatggcggtg ccgaaaaggt ggttggggaa
     2461 gcattaaccg gtctgcgaga gaaggtcttt ctcgtctcta aagtctatcc gtggaatgct
     2521 ggcgggcaaa aagcgataaa tgcatgcgaa gccagtttac gccgtctcaa tactgactat
     2581 ctcgatctct acttattaca ctggtctggc agtttcgctt ttgaagagac tgttgctgcg
     2641 atggaaaaat tgatcgccca gggaaaaatc cgtcgctggg gcgtttctaa ccttggttat
     2701 gccgatatgc aggaactctg gcagctgccg gggggaaatc agtgtgccac taatcaggtg
     2761 ctttaccatc tcggttcacg agggattgag tacgatctac tcccctggtg ccagcaacag
     2821 cagatgccgg tgatggctta cagtccatta gcccaggccg ggcggttgcg caatggactg
     2881 ttaaaaaacg cggtagtcaa cgaaattgca catgtccaac aacatgcagc tgcgcttgag
     2941 gtcgaacttt cgtcagcgga attagctatg ctggataagg catatccggc accaaaagga
     3001 aaaactgcgc tggatatggt gtgatgttga tacgtgccgg atgcatcgca cccggcacac
     3061 aacattaacg caaattaacg tttggcaacg cgaatggatt gcgccagatg tgcaggtttc
     3121 tctttggtga ctttttgcgt ttctgaagcg taagccgttt ctacacaaac aaaggtcttg
     3181 tagccatcgt ccggcatgtc gcccatgcta actgaaagcg ccggtcccgg gttccagccg
     3241 acaacgttca gatgatgctg gtggcctacg gcgataatac gattcagcgc ttcatcatta
     3301 atcacgctgc aatcttgcgg attcagatac acgcgatcgg tacggtcagg gaaggtctga
     3361 ataccgtcgg tcagtacatc ttctttcgcg tcattcactt tatcaatgaa gcgatcgccc
     3421 agcccactga cgcttacctt agcgatatca cccacgttaa agtaggtatg cagggcagag
     3481 gtggtttcaa attcgccatg tgattcaaga tcgatttcac aggttttacc cacgcggaaa
     3541 tgcgctaaca gcgtaaagtc gtgcggccag aattttttcg tctcttcgct ttgtgtcaat
     3601 tcaaaagtca gcgctacgcc atcagcatct tcatgatgtg atttcagcgt ccacggcagg
     3661 ttgcgggcaa aaccgtgcgc aggcagacct tgttgtgccg ccggaccaaa ccacggccag
     3721 caaaccggta cgccaccgcg aatagcgacg ccatttttga acggtgtgtt gttgctcaac
     3781 cacagaactt cttcttcacc cgcaggtttc cacgagagaa ggtgtgcgcc ctgtaatgca
     3841 aaagaggctt ttacctgggg atgatcgacc acaatgaggt ccagttcatc cagtttacga
     3901 cgggagagga caggggagat ttgttcgatg accggaaggg caaaaatttt cttaatcatg
     3961 acgcagtcct ttaacttcat tttatcaggt aaaaaaaaga gcgaccgaag tcgctctttt
     4021 tagatcacag tgtcatctca acttatttgg agatgtgagc gatcaggtcc agaactttgt
     4081 tggagtaacc ggtttcgttg tcgtaccagg ataccagttt cacgaagttg tcgttcagag
     4141 cgataccagc tttagcatcg aacacggaag tgcaaacttc gccgttgaaa tcggtagata
     4201 ctacgtcatc ttcggtgtag cccagaacgc ctttcatttc gccttcagca gcagctttaa
     4261 cggcagcttt gatctgctcg taagttgcag ctttttccag acgaacggtc aggtcaacta
     4321 cggatacgtt cggggtcgga acgcggaacg ccataccagt cagtttgcca ttcagttctg
     4381 gcagtacttt acctacagct ttagcagcac cggtagagga cgggatgatg ttctgggaag
     4441 cgccgcggcc gccgcgccag tctttgtgag acgggccatc aacggttttc tgagtagcgg
     4501 tagtagcgtg aacggtggtc atcagacctt cgatgatgcc gaagttatcg ttgataactt
     4561 tagccagcgg agccaggcag ttggtggtgc aggaagcgtt ggaaacgatg tcctggccag
     4621 catatttgtc gaagttagcg cctttaacga acatcggagt gttgtcttta gacggaccag
     4681 tcataaccac tttcttcgca ccagcggtga tgtgtttacg agcagtttcg tcagtcagga
     4741 acagaccagt tgcttcagcg acaacgtcaa cacccacttc gtcccatttc aggttagccg
     4801 gatcacgttc agcggtaaca cggatttttt taccgttaac gatcagatga ccgtctttca
     4861 cttcaacggt accgtcgaaa cggccgtgag tggagtcata tttcagcatg tatgccatgt
     4921 aatcagcgtc taacaggtcg ttgattgcaa cgatctcgat gtcagaacgt ttctgagcag
     4981 cacggaaaac aatgcgaccg atacggccaa aaccgttgat acctactttg atagtcatat
     5041 attccaccag ctatttgtta gtgaataaaa ggttgcctgt aaaattacaa aaaccttacg
     5101 cagcgtcaag cggaatcgtg tcaatcattg cgacaaatca atcctgtgcc taagcattac
     5161 gcgactgact cgcctcactt ttcctttggg cttgagacca catggggtcg gcgccccgaa
     5221 ttttaaaggg caattacgat aaaaatgtga ttttcatcac gatttaggtg cagccatttc
     5281 gtcgagatca agtttaggac aaaattttgc cagttggtgt taatgttttg ttagaatcag
     5341 tcacgtaatg tgagcacgat taaagtgaga tgtgagtaaa tggcgaataa accttcggca
     5401 gaagaactga aaaaaaattt gtccgagatg cagttttacg tgacgcagaa tcatgggaca
     5461 gaaccgccat ttacgggtcg tttactgcat aacaagcgtg acggcgtata tcactgtttg
     5521 atctgcgatg ccccgctgtt tcattccgaa accaagtatg attccggctg tggctggccc
     5581 agtttctacg aaccggtgag tgaagaatcc attcgttata tcaaagactt gtcacatgga
     5641 atgcagcgca tagaaattcg ttgcggtaac tgtgatgccc atctggggca tgtctttccc
     5701 gacgggccgc aaccaacagg cgaacgttat tgtgttaact ctgcttcttt acgcttcact
     5761 gatggcgaaa acggcgaaga aatcaacggt tgaaaaaacg attcagcaac ttattccaca
     5821 ggagcggaaa aattatgaat cttgatgaca ttatcaacag catgacgcct gaggtatacc
     5881 agcgtttgtc gaccgccgtt gaactgggga aatggcctga tggcgttgcg ttgaccgagg
     5941 aacaaaaaga gaattgcctg caactggtga tgctgtggca agcccgccat aataccgaag
     6001 cacagcatat gacaattgac acaaatggtc agatggtcat gaagagcaaa cagcagttaa
     6061 aagaagattt tggtatctca gcaaagccta ttgccatgtt taagtaagtg gttcatcccc
     6121 tttttgccgc ggctatgaag gggatagcca acaatgaccc cacgactcgt ctcaattatc
     6181 tcgacccgac ctggctaagt cccagtccga tctcttgcag tcgcgttcac gcctgaatga
     6241 cttctcctga acttaagtct cagacctatt tggccggtaa tccctctcga atttgctctt
     6301 tttgcgtccc gccttccacc aacctgtaac agacccagcc attttctgcg taattgtgat
     6361 ttgagttgta aaatgcaggt cattatctgt gttgtgcaga tttaatattg acctcaaaaa
     6421 tcatgtgggt aagtatgatg gccatctaag caaaacaaaa cacagaggat gacgcaatga
     6481 aagcactggc tcggtttggc aaggcctttg gcggctacaa gatgattcat gtcccacaac
     6541 ccatttgtgg cccggaagac gtagtgactg aaattaaagc cgcggcaatc tgcggcgcag
     6601 acatgaagca ctacaatgtc gatagcggtt ctgatgagtt taactctatc cgcggccatg
     6661 agttcgcagg ttgtattgcg caggttggtg aaaaagtcaa agactggaaa gtggggcaac
     6721 gcgtcgtgtc ggataacagc ggtcacgttt gcggcgtttg tccggcctgt gaacagggtg
     6781 attttctgtg ttgtacagaa aaagtaaacc ttggtctgga taacaatacc tggggcggtg
     6841 gtttttccaa atattgtctg gttcctggtg aaattctcaa aattcatcgt catgcgttgt
     6901 gggaaatccc tgatggtgtt gattatgagg acgcagccgt acttgaccct atctgtaatg
     6961 cctataaatc catcgcgcag caatcgaaat tccttcctgg tcaggatgtt gtcgtcattg
     7021 gcactggccc actcgggctg ttttccgtac aaatggcgcg gattatgggg gcggtaaata
     7081 tcgtcgtcgt tggtctgcaa gaagatgtgg cggtccgctt cccggttgca aaagaactgg
     7141 gggcgacggc agtagtaaat ggttctaccg aagatgtggt ggcgcgctgc cagcaaattt
     7201 gtggcaaaga taatctggga ctggtgattg aatgctccgg tgccaatatc gcactgaaac
     7261 aagccatcga aatgctccgc ccgaatgggg aagtggtacg cgttggaatg ggcttcaaac
     7321 ctcttgattt ctcgattaat gacattaccg cctggaataa aagcatcatt gggcatatgg
     7381 cctatgactc cacctcatgg cgtaacgcta tccggctatt agccagcggc gctatcaaag
     7441 tcaaaccgat gatcacgcat cgtatcggcc tgtcgcaatg gcgcgaaggg tttgatgcga
     7501 tggtcgataa aaccgcaatc aaagtgatca tgacttacga ctttgatgaa taactactct
     7561 cctgaaggaa cgttatcgca tggaacagat aacaaaaccg cattgtggtg cacggctgga
     7621 tcgcttaccg gattgccgct ggcattcgtc aatgtttgcc atcgtcgcgt ttggcttgct
     7681 tgtctgctgg agtaatgccg ttggtggctt gatcctcgcg cagctgaaag cgttgggctg
     7741 gacagataat tccactacag ccacattctc agcaatcacg accgccggaa tgtttctcgg
     7801 tgcgctggtg ggcggcatca ttggcgacaa aaccggtcgc agaaatgcgt tcatcctcta
     7861 tgaggccatt catattgcct cgatggttgt cggtgctttc tcaccgaata tggattttct
     7921 tattgcctgc cgttttgtga tggacgttgg gctgggagct ttactggtta cactgtttgc
     7981 tggtttcacc gaatatatgc ccggtagaaa tcgcggaacg tggtccagtc gggtttcctt
     8041 tattggcaac tggtcatatc cgctctgctc actgattgcg atgggactca cgccgctgat
     8101 tagtgcagag tggaactggc gggtacaact gcttattcct gcaatattgt cgcttatcgc
     8161 tacggcgctg gcctggcgct actttcctga atccccgcgc tggctggaat cgcgcggacg
     8221 gtatcaggaa gccgagaaag tcatgcggag tatagaagaa ggcgtcatac gccagacggg
     8281 taaacctttg ccgcctgtgg ttattgctga tgacggtaaa gcgccacaag cggtgccgta
     8341 ttcagcctta ctgacaggag tattactgaa acgcgtgata ttaggctctt gtgtgctgat
     8401 taccatgaac gttgtgcagt acacactaat taactggttg ccaacaatat tcatgaccca
     8461 ggggattaat ttaaaagact cgattgtttt aaataccatg agtatgtttg gcgcgccatt
     8521 tggtattttt attgccatgc tggtgatgga taaaattccg cgtaaaacaa tgggtgtggg
     8581 gctattaatc ctgattgcgg tgctcggata tatctattca ctgcaaacca gtatgttgct
     8641 cataacgctg attggtttct tcctgattac tttcgtctat atgtacgttt gctatgcctc
     8701 ggcagtgtat gtccctgaaa tctggccgac agaggccaaa ctccgtggct ccggtctggc
     8761 gaatgcggta gggcgaatca gtggtattgc cgcaccttat gccgttgcag tgctgctcag
     8821 tagttatggt gtaacgggag tctttattct tctgggggct gtttcaaata ttgtcgcaat
     8881 tgctatcgcc accattggaa ttgaaaccaa aggtgtctcc gttgaaagtt taagtattga
     8941 tgcagtagcc aataaataat acgggtggat tcaaaatgaa aaattcaaaa gcaatattgc
     9001 aggtgccggg cacaatgaaa attatttcag cagaaatacc agtgcctaaa gaagatgaag
     9061 ttttgattaa agtagaatat gtcggtattt gtggttcaga tgtacatggt tttgaatcag
     9121 gcccgtttat tccgcctaaa gacccaaatc aagaaattgg cctgggtcat gaatgcgccg
     9181 ggacggttgt ggctgtggga agccgcgtgc gcaaatttaa accgggggat cgggtaaata
     9241 tcgaacctgg cgttccttgc ggtcactgtc gttactgtct ggaaggcaaa tataacatct
     9301 gcccggacgt tgattttatg gcgacacaac ccaactaccg cggcgcatta acgcactatc
     9361 tgtgtcatcc ggagagcttt acttacaaac tgcccgacaa tatggacacg atggaagggg
     9421 cgctggtgga gcctgccgca gtcgggatgc atgccgcgat gctggcagat gttaaaccgg
     9481 gtaagaagat aattattctg ggagcaggtt gtattggttt gatgacgttg caagcgtgca
     9541 aatgcctggg cgcaacggaa attgccgtcg ttgatgtact ggaaaaacgt ctggcaatag
     9601 gggaacagcc tggtgcgaca gtggttatta atggcgcaaa agaggacact attgcacgct
     9661 gtcagcaatt taccgaagac atgggcgcag atattgtttt cgaaacagcg ggttctgcgg
     9721 tcaccgttaa acaggcacct ttaatctggt aatgcgcggc ggtaaaatta tgattgttgg
     9781 tactgtaccc ggcgattcgg caatcaattt cctcaaaatc aatcgcgaag tcactatcca
     9841 gacggtattc cgctatgcca atcgttatcc ggtcacgatt gaagctattt cttcagggcg
     9901 attcgatgtg aaatcgatgg tgacgcatat ttacgattat cgggatgtac aacaggcatt
     9961 tgaagagtca gttaacaaca aacgcgacat tattaaaggc gttattaaaa ttagcgatta
    10021 attaatacgg gagaacagaa catgctcgca gatatcagat attgggaaaa cgatgccaca
    10081 aataaatatt atgcaattgc ccattttaac gtatggaatg cagaaatgtt gatgggcgtt
    10141 aaagacgcag ccgaagaagc gaaatccccg gttattattt cttttagtac aggttttgtc
    10201 ggtaacacct catttgaaga tttctctcac atgatggtat caatggcaca aaaagcaacg
    10261 gtgccggtaa taactcattg ggatcatggt cggagtattg agattattca taacgcctgg
    10321 actcatggta tgaattcatt aatgcgtgat gcttctgcat ttgatttcga agaaaatatt
    10381 cgtttaacca aagaggctgt tgatttcttt catccgctgg gtattccggt agaggcggaa
    10441 ttagggcatg tcggtaatga aaccgtttat gaggaagcgc tggcgggcta tcactatacc
    10501 gatcctgacc aggcggctga atttgttgaa cgtacgggct gtgactcatt ggctgtcgcc
    10561 atcggcaacc agcatggggt ttatacgtca gagccgcaat tgaactttga ggtcgtcaaa
    10621 cgcgtacgcg atgctgtttc tgttccgctg gttttgcatg gcgcatcggg gatcagtgat
    10681 gccgacatta aaactgcaat ttcgttgggt attgcgaaaa tcaacattca tacggagctc
    10741 tgtcaggccg caatggtagc agtgaaagaa aaccaggatc agcctttcct gcatttagaa
    10801 cgtgaagtac gtaaagctgt aaaagaacgg gcattggaaa aaattgaact gttcggttct
    10861 gatggcaaag cggaataatg atatggataa tctcgacgtt atttgtatag gtgccgctat
    10921 tgttgatatt ccattacaac cggtcagtaa aaatatcttt gatgtagatt cttatcctct
    10981 tgaaagaatc gcaatgagca ccgggggcga tgcaataaat gaagcaacaa ttatttctcg
    11041 tctggggcat cgtaccgcat taatgagccg cattggtaaa gatgcctcag ggcaatttat
    11101 tctcgaccat tgtcgcaaag agaatattga tattcaaagc ctgaaacagg acgtcagcat
    11161 agatacatct attaacgttg gactggtgac ggaggatggc gagcggacgt ttgtcacgaa
    11221 ccgtaacggc agtttgtgga aattaaatat tgacgatgtt gattttgcgc ggtttcctca
    11281 ggcgaaatta ttatcactgg ccagtatttt caacagtcct ctattggatg gtaaagcgct
    11341 aacagaaatt tttacgcaag cgaaagcccg gcagatgatc atttgtgccg atatgatcaa
    11401 accgcggttg aatgaaacgc tggatgatat ttgcgaagca ttgagttatg tcgattatct
    11461 gtttccaaat tttgccgagg aaaaattact cacagggaaa gagacactgg atgaaattgc
    11521 tgactgcttt cttgcgtgcg gcgtaaaaac ggtggtgatt aaaacgggta aagacggctg
    11581 ctttatcaag cgtggtgaca tgacgatgaa ggtgccggcg gtcgcaggaa taaccgccat
    11641 cgacaccatt ggcgcgggcg ataactttgc ttcaggtttt attgcggcac tgttagaagg
    11701 caaaaatctg cgtgaatgcg cacgctttgc caatgcaacg gcggctatct cggttctaag
    11761 cgtcggtgcc accaccggcg taaaaaacag aaagctggtc gaacaattgc tggaagaata
    11821 cgaaggataa tgaaggcaaa tgaaaaagat acctttaggc acgacggata taacgctttc
    11881 gcgaatgggg ttggggacat gggccattgg cggcggtcct gcatggaatg gcgatctcga
    11941 tcggcaaata tgtattgata cgattcttga agcccatcgt tgtggcatta atctgattga
    12001 tactgcgcta ggatataact ttggcaatag tgaagttatc gtcggtcagg cgttaaaaaa
    12061 actgccccgt gaacaggttg tagtagaaac caaatgcggc attgtctggg aacgaaaagg
    12121 aagtttattc aacaaagttg gcgatcggca gttgtataaa aacctttccc cggaatctat
    12181 ccgcgaagag gtagaagcca gcttgcaacg tctgggtatt gattacatcg atatctatat
    12241 gacgcactgg cagtcggtgc cgccattttt tacgccgata gctgaaactg tcgcagtgct
    12301 taatgagtta aaggctgaag ggaaaattcg cgctataggc gctgctaacg tcgatgctga
    12361 ccatatccgc gagtatctgc aatatggtga actggatatt attcaggcga aatacagtat
    12421 tctcgaccgg gcaatggaaa acgaactgct gccgctatgt cgtgataatg gcattgtggt
    12481 tcaggtttat tccccgctag agcagggatt gttgaccggc actatcactc gtgattacgt
    12541 tccgggcggc gctcgggcaa ataaagtctg gttccagcgt gaaaacatgc tgaaagtgat
    12601 tgatatgctt gaacagtggc agccactttg tgctcgttat cagtgcacaa ttcccactct
    12661 ggcactggcg tggatattaa aacagagtga tttaatctcc attcttagtg gggctactgc
    12721 accggaacag gtacgcgaaa atgtcgcggc actgaatatc aacttatcgg atgcagacgc
    12781 aacattgatg agggaaatgg cagaggccct ggagcgttaa atactcaaca cgattcacct
    12841 ctgtttctgt gaaaactgtg atgaaaatct gtgattcaaa taggttattt tgaaagtaaa
    12901 catcggttat gcgataatcg cgctaatgtg atgtaaaaag atacaggggt gtaatcgtgg
    12961 cggcaaaaga caggattcag gcaattaagc aaatggttgc caacgataaa aaggtgacag
    13021 tctcaaattt gagtgggatt tttcaggtaa ccgaagaaac cattcgccgc gatcttgaga
    13081 agctggaaga tgaaggcttt ttgaccagaa cctatggtgg tgctgtttta aatacagcgg
    13141 tgttgacgga gaatatccat ttttataagc gcgcttcatc gttttatgaa gagaagctgc
    13201 ttattgcacg caaggcacta ccctttatcg acaataaaac caccatggca gccgattcca
    13261 gtagtaccgt aatggaattg ctcaaattgt tacaggaccg tagtgacctg acgttgctaa
    13321 ccaactcggc ggaagcgatt catgttctgg ctcagtcaga aattaaagtc gtttcaacgg
    13381 gtggggaact aaacaaaaat acactttccc tgcaaggaag aattactaaa gagatcatca
    13441 gccgctacca tgtcgatatc atggtaatga gttgtaaagg tcttgatatt aacagcggcg
    13501 cgctggactc taatgaagcg gaagctgaaa tcaaaaagac aatgatccgt caggcgacag
    13561 aagttgcgtt attggttgat cactctaagt ttgatcgtaa agcttttgtc cagttagctg
    13621 actttagtca tattaattat ataataactg ataaatcacc gggtgcagag tggatagcat
    13681 tttgcaaaga caataatatc cagctggtgt ggtaatttaa gcacacgttg tcaatattat
    13741 ttgttcctgc gactggaaat aaaatgatga taaagatgcg gcaagtgaat ttgccgcaat
    13801 tgctaccttt tatagcaaat ccgaggcacc caatggaaca atatgatcaa attggcgcaa
    13861 gactggaccg cttgcctttg gcccggtttc attatcgtat atttggtata ataagcttta
    13921 gtctgttatt aacggggttt ttgagttact caggtaatgt cgtcttagca aagctggtaa
    13981 gcaatggatg gtcaaataat ttcctctatg ccgcctttac ctcggcatta atgtttggtt
    14041 atttcatcgg ctcacttact ggtgggttta ttggtgacta ctttgggcgg cgcagggcgt
    14101 ttcgcataaa tcttctcatc gtcggtattg ctgcaacagg ggccgctttt gtccctgata
    14161 tgtactggct cattttcttt cgcttcctga tgggaacagg aatgggggcg ctgattatgg
    14221 ttggctatgc ctcatttacg gagtttatcc ccgcgacggt gcgtggaaaa tggtccgcgc
    14281 ggctctcatt tgttggtaac tggtcgccca tgctgtctgc ggcgataggc gtggtggtta
    14341 tcgctttttt tagttggcga ataatgtttc ttctgggtgg tattggcata ctgttagcct
    14401 ggtttctctc aggtaaatac tttatcgagt cgccacgatg gctggaaggg aaagggcaaa
    14461 tcgccggtgc agaaagccaa cttcgtgaag tagagcagca aattgaaagg gagaagagta
    14521 ttcgtttacc ccagcttact ttgaaccaga gcaacagcaa ggttaaggta atcaagggta
    14581 ccttcaggct cctgtttaaa ggggaaatgt tacgacgtac attagttgcg ataactgttt
    14641 taattgcaat gaacatttcg ctttatacca ttaccgtatg gataccgacc atatttgtta
    14701 actccggcat tgatgtcgat aaatcaatat taatgaccgc tgttattatg attggtgctc
    14761 cggtaggaat atttattgcg gcattaatta ttgatcattt tcctcgtcga ttatttggct
    14821 ccgccttact tattattatt gccgtgttag gctatatcta ttcaagtcag actacagagt
    14881 gggcgatttt aatctatggt ctggtgatga tcttcttttt atacatgtat gtttgcttcg
    14941 cgtcggcggt ttatatcccg gagctttggc caacgcattt acgcctgcgc ggttcgggtt
    15001 tcgttaatgc cgtcggacgg atcgtcgcag ttttcacgcc ctatggcgtt gcggcattat
    15061 taacacatta tgggtcgatc acggtgttta tggtgcttgg tgtcatgtta gtgctctgtg
    15121 cgctggttct ctccattttt ggcatcgaaa cgcggaaggt gtcgctggaa gagatttctg
    15181 aggtgaatta aatctttcgt tgatggcatc aattgccaga tgtaccactg acgtgtttta
    15241 tttggcctac aatgccagta cagaattgta ggccagcgta aaattacccc tgcgtctctt
    15301 cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgtg
    15361 cactgtcctg gggctggata ttcacgccac gacagccatc ggtaatcacg tttaccttat
    15421 aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca
    15481 taacgatcaa ttcatcgatt tcatgatcgc gcaaccagtc atcaagcgcg gttttctgcc
    15541 gacgtccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga
    15601 acaccgctgc gatctctttt tgattcagta acggatgtaa ttgtgcgcct tcactgttct
    15661 gcacacagtg ttcaggccag aaagtttgcc gcaggccgcc gagttggcct ggcgtataag
    15721 gctctacgcc gtgctggctg gcaaagctgc cgtgattcgc cggatgccag tcctggctgg
    15781 cgataaccgc ttctccgcgc gactggcacc agtcaataag gcggttagca acctccaccg
    15841 tactgtcacc ttccggtacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga
    15901 ccagtaacag ggtgcgatgg ggcattgcag tctccttaat catccggcgt cagttcgccg
    15961 cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg
    16021 taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgtcaat aacgccggca
    16081 tgggcgaggg cgttaccggt ggcataacca ctcatattca ctttaccgga catacattgt
    16141 gtcaggttga ccaccacaat accgcgatcg ctggcttctt gtaattcctg caggaaggct
    16201 ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt caccggttgg
    16261 cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac cacgccaatt
    16321 ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta
    16381 ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac
    16441 gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg
    16501 aaaaagagcg ttacttcgtt gatcggataa ttcggcgcaa cgtacaacgc attcagcaga
    16561 ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc
    16621 ggtttaccga gattctcgag catgaacgac agcgcagagg cggtatacgc catcgtgtcg
    16681 gtaccgtgta gaatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca
    16741 atatgctgcc agtcttccgg cgtcatatca gaagaatcca tcaacggcgt atattcatga
    16801 atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt
    16861 agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc
    16921 gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag
    16981 caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg
    17041 gggcgagaca gcgggaagag gattcgtcga ggtaaaaaat aatgtggccg acgatctgtc
    17101 ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt
    17161 ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga
    17221 ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc
    17281 agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg
    17341 ccactgtttc acttttgcga gctctgctgc tttggcaacc gcatcatcga aatccccgag
    17401 actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat
    17461 tttatcaatt tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt
    17521 atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt
    17581 gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc
    17641 cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct
    17701 ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat
    17761 gccgcccatc gatacaacca cagacttacc cgctgcccgg gctgctgcca gttcagcgcg
    17821 aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt
    17881 cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt
    17941 cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc
    18001 accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt
    18061 atcagtctta ctccagccga actctttggt cagagctttt tcgatttcgg cactcgatgc
    18121 cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt
    18181 taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga tctgccggtt
    18241 agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc
    18301 gcgggctgcc ggtgacatat catcacgaat aaacggttca acggcagatt tatacgtacc
    18361 cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc
    18421 gttggtagca aaaccgtgca gatcaaccac gccttgcggt gacagccaaa ttttattggc
    18481 gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt
    18541 cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc
    18601 gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt ccttcgcctg
    18661 gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc
    18721 aagcagctgg cggcttaatt tactaaaccg ctgagaactg tctggtttat cgacgatcac
    18781 accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact
    18841 gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac
    18901 catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg
    18961 ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat
    19021 gtggttatcg taatgaccca accggcactt gtcagcagga attgcggact gtgctgtaac
    19081 aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga
    19141 tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac
    19201 gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat
    19261 gcaaccgtgg catttttttg tgattgaagg ggaagggtgc gagcgtttca gcgccgtact
    19321 ggaacagggg gcgattgctg ccggtagtga tgataaagct atcgacaaag cccgtaatgc
    19381 gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcaaagaaa atcataaagt
    19441 cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc
    19501 cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt
    19561 gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac
    19621 gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta
    19681 tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac
    19741 tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg
    19801 tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt
    19861 aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat
    19921 ccgaatttgc ttgtgggtaa tgaaacccgc gacgatgcgg cggtgtacga tctgggcaat
    19981 ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat
    20041 tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg
    20101 attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc
    20161 gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt
    20221 cactccatcg atgcgccgga gccgattttt ggtctggcgg taacggggat cgtaccgacc
    20281 gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct gacgaaaccg
    20341 ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgttgaaacc agaacatcag
    20401 ggactggcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc
    20461 gaaggcgtaa aagcgatgac cgacgttacg ggctttggtc tgctgggcca cttgagcgaa
    20521 atgtgtcagg gggctggtgt gcaggcacgc gtcgactatg aagcgatccc gaaactcccc
    20581 ggtgttgaag agtacattaa gttgggcgca gtacctggcg gcactgaacg taactttgcc
    20641 agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatctgct gtgcgatccg
    20701 caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga ggttaaaact
    20761 acagccgccg agtttggcat tgaactgacg gcgattggcg aactggtgcc agcgcgcggc
    20821 ggtcgtgcca tggttgagat ccgttaattt aatgcggttg tttattgccg aaaaaccgag
    20881 tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat
    20941 cgagtgcggt aatggtcagg tggtgacttg gtgtatcggt cacctgcttg agcaggcgca
    21001 gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattgtccc
    21061 ggaaaagtgg caattacagc cacgaccctc cgtgaccaaa caacttaacg tcatcaaacg
    21121 cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaatt
    21181 gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca
    21241 gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgatcg accgtcttcg
    21301 ttccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg
    21361 gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca
    21421 gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga
    21481 tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac
    21541 acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca
    21601 ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag
    21661 tggtcaaccg gctattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct
    21721 gccgttttcg ctttcggcgt tgcagattga agcggcaaaa cgcttcggtc tgagcgcgca
    21781 gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg
    21841 ttccgattgt cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc
    21901 catcagcgtt catgcaccgg atctgttgcc gcagccagtg gtagatccag atatacgcaa
    21961 ccgctgttgg gatgacaaaa aggtcgatgc gcaccacgcc atcatcccga ccgcacggag
    22021 ttctgcgatc aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcaata
    22081 cctgatgcag ttctgcccgg atgcggtgtt ccgcaagtgt gttatcgaac tggacattgc
    22141 caaaggcaaa tttgtcgcta aagcccgttt tcttgctgaa gcaggctggc gcacgctgtt
    22201 aggcagcaaa gagcgcgatg aagaaaacga cggcacgcca ttgcctgtgg tggcgaaagg
    22261 cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca
    22321 ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa
    22381 agatctgaaa aaggtccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg
    22441 gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc
    22501 caccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat
    22561 gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga
    22621 ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc
    22681 ggtgcggcag tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa
    22741 ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag ccggaagcgg
    22801 ggcgatagcg taagcgagcg aatctttcgt gctattcgag tcatattctg aaatatccag
    22861 cggatcaaga aagttcgttg gatatttttt tgcatggata aaattatcgc ctctaaagta
    22921 tgtaataaca gggaatgtga tgaaaaaagt tttattgcaa aaccatcctg ggagcgagaa
    22981 gtattctttt aatggctggg gaatatttaa tagtaatttt gaacgggtga ttaaagaaaa
    23041 taaggccatg ctgctttgta agtgggggtt ttatttaaca tgtgttgtcg ctgtaatgtt
    23101 tgtattcgca gcgataacat ccaacggttt gaatgaaaga ggcctgatta ccgcgggatg
    23161 ctcttttctt tatctattaa ttatgatggg gcttattgtt cgggccggtt ttaaagcaaa
    23221 aaaagaacaa ctgcattatt atcaggctaa aggtattgag ccgctcagta tcgagaagtt
    23281 acaggcgcta caattgatcg caccttatcg attctatcat aagcaatggt ctgaaacgct
    23341 agagttctgg ccgcgaaagc ctgaacctgg caaagatacc ttccaatatc atgtgcttcc
    23401 ttttgactcg atcgatatca tcagtaaaag acgcgagtct ttagagaatc aatggggtat
    23461 cgaagatagc gaaagttatt gtgccttaat ggagcatttt ctttctggcg accatggagc
    23521 caataccttt aaagcaaaca tggaggaagc cccagagcag gttatcgcct tgttgaataa
    23581 atttgctgtt tttccctcag actatatctc tgattgcgct aataatcgct ccggtaaatc
    23641 ctcggcgaag ctaatatggg cggcggaatt atcatggatg atctcgatat caagcacagc
    23701 ttttcaaaac gggacaattg aagaagaact ggcctggcat tatataatgc ttgcttctcg
    23761 aaaggcgcac gagttgttcg aaagcgaaga agattatcaa aaaaatagtc taatgggatt
    23821 tctttactgg catatctgct gctatcgcag aaagttaacg gatgctgaac ttgaagcgtg
    23881 ttatcgttac gacaagcagt tttgggagca ctacagtaag aaatgccgtt ggcccataag
    23941 aaatgttccc tggggagcat catccgttaa atactcataa acgcctgaaa ttttgccggg
    24001 ggcgctgcgc ttgaccggcc tacattagag cgtaactgca atgaattgtg cccatctgta
    24061 ggcctgataa gcgtagcgcc atcaggcatt tacaacttaa atcacaccct gcgccagcat
    24121 cgcatcggca accttcacaa aaccggcaat gttcgcgccc tgcacgtagt tggtttgctc
    24181 accttcacca ccatgctcaa cacaggcatg gtggatatcc agcatgatgt gatgcaaacg
    24241 tgcgtcaact ttctcggctt tccagcccag gcgcgcagcg ttttgtgcca tttccaggcc
    24301 cgacgttgct acaccgccag cattagccgc tttacccggt gcaaacagta cgcctgcctg
    24361 ctggaacagt tcagtcgctt cgatggtggt cggcatatat gccccttcag cgacggcttt
    24421 aacgccattg gcgataagct gatgcgcggc gtcaacatcc agttcgttct gggtggcgca
    24481 aggcagggcg atatcaaccg gtacagacca cggctgttgg ccttcgagat agaccagacc
    24541 aaattctttg gcgtaatctg ccgctcgacc atcgcggctg gctttgattt cgataagacg
    24601 tgccagtttc tctttcgtga atccgctttc atcaactaca gtgccgctgg agtctgacgc
    24661 agtgatcaca cgagcaccaa attccatcgc tttttcgata gcgtactggg cgacgttgcc
    24721 ggagccagaa acggaaacgc tcatcccttc aaaacccata ccgtggcgtt ttaacattgc
    24781 ttctgtgaaa taaaccagac cgtagccggt agcttccggg cgaataagac taccgccaaa
    24841 tgaaaggccc ttaccggtga agacgcaggc ggtattgttg gagagctttt tcatcatccc
    24901 cgccataaag ccgacttcac gaccaccaac gccgatatca cctgccggaa cgtcggtatc
    24961 cgcgcccagg tggcgataca gttcagtcat cagcgtctgg caaaaacgca tcactttacc
    25021 ttcgcttttt cctttcggat cgaaatcgct gccgccttta ccaccgccca tcggcagggt
    25081 agtcagggca tttttgaagg tttgttcaaa gccgaggaat ttgagaatgg aaa
//
LOCUS       DAJTYH010000060        24272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTYH010000060 DAJTYH010000000
VERSION     DAJTYH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            655..2583
                     /gene="thrS"
                     /locus_tag="OO609_002382"
     CDS             655..2583
                     /gene="thrS"
                     /locus_tag="OO609_002382"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6633768.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2587..3129
                     /gene="infC"
                     /locus_tag="OO609_002383"
     CDS             2587..3129
                     /gene="infC"
                     /locus_tag="OO609_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6633769.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3226..3423
                     /gene="rpmI"
                     /locus_tag="OO609_002384"
     CDS             3226..3423
                     /gene="rpmI"
                     /locus_tag="OO609_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6633770.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3476..3832
                     /gene="rplT"
                     /locus_tag="OO609_002385"
     CDS             3476..3832
                     /gene="rplT"
                     /locus_tag="OO609_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6633771.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3949..4073
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3955..3999
                     /gene="pheM"
                     /locus_tag="OO609_002386"
     CDS             3955..3999
                     /gene="pheM"
                     /locus_tag="OO609_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6633772.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4282..5265
                     /gene="pheS"
                     /locus_tag="OO609_002387"
     CDS             4282..5265
                     /gene="pheS"
                     /locus_tag="OO609_002387"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6633773.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5280..7667
                     /gene="pheT"
                     /locus_tag="OO609_002388"
     CDS             5280..7667
                     /gene="pheT"
                     /locus_tag="OO609_002388"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6633774.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7672..7971
                     /gene="ihfA"
                     /locus_tag="OO609_002389"
     CDS             7672..7971
                     /gene="ihfA"
                     /locus_tag="OO609_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6633775.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8072..9052
                     /gene="btuC"
                     /locus_tag="OO609_002390"
     CDS             8072..9052
                     /gene="btuC"
                     /locus_tag="OO609_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6633776.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9115..9666
                     /gene="btuE"
                     /locus_tag="OO609_002391"
     CDS             9115..9666
                     /gene="btuE"
                     /locus_tag="OO609_002391"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6633777.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9666..10415
                     /gene="btuD"
                     /locus_tag="OO609_002392"
     CDS             9666..10415
                     /gene="btuD"
                     /locus_tag="OO609_002392"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6633778.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10493..10957
                     /locus_tag="OO609_002393"
     CDS             10493..10957
                     /locus_tag="OO609_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6633779.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11204..11917
                     /gene="ydiV"
                     /locus_tag="OO609_002394"
     CDS             11204..11917
                     /gene="ydiV"
                     /locus_tag="OO609_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6633780.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11981..13417
                     /locus_tag="OO609_002395"
     CDS             11981..13417
                     /locus_tag="OO609_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6633781.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13421..13612)
                     /gene="hemP"
                     /locus_tag="OO609_002396"
     CDS             complement(13421..13612)
                     /gene="hemP"
                     /locus_tag="OO609_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6633782.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13744..14790)
                     /gene="aroH"
                     /locus_tag="OO609_002397"
     CDS             complement(13744..14790)
                     /gene="aroH"
                     /locus_tag="OO609_002397"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6633783.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14947..15780)
                     /gene="ppsR"
                     /locus_tag="OO609_002398"
     CDS             complement(14947..15780)
                     /gene="ppsR"
                     /locus_tag="OO609_002398"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6633784.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16112..18490
                     /gene="ppsA"
                     /locus_tag="OO609_002399"
     CDS             16112..18490
                     /gene="ppsA"
                     /locus_tag="OO609_002399"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6633785.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18547..20193)
                     /gene="fadK"
                     /locus_tag="OO609_002400"
     CDS             complement(18547..20193)
                     /gene="fadK"
                     /locus_tag="OO609_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6633786.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20250..20543)
                     /locus_tag="OO609_002401"
     CDS             complement(20250..20543)
                     /locus_tag="OO609_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6633787.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20540..21829)
                     /locus_tag="OO609_002402"
     CDS             complement(20540..21829)
                     /locus_tag="OO609_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6633788.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21885..22823)
                     /locus_tag="OO609_002403"
     CDS             complement(21885..22823)
                     /locus_tag="OO609_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6633789.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22843..23607)
                     /locus_tag="OO609_002404"
     CDS             complement(22843..23607)
                     /locus_tag="OO609_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6633790.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            23926..>24272
                     /locus_tag="OO609_002405"
     CDS             23926..>24272
                     /locus_tag="OO609_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6633791.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCP"
BASE COUNT         5982 a         5897 c         6397 g         5996 t
ORIGIN      
        1 tcaagtaggg tgatgagctg gcgcgcaaat ttattaatag gctctgatga aggtgttggt
       61 atgtcgctat cgatgtgcaa tctatgagga gacttataat gagttctgat aatgatatcg
      121 ttttgactca taatctattc gccttatata tatctggagg ttttgaggat gatgattaat
      181 tattatcgcc agaacgaaat gtgcacgaaa aattgttata agaattgtgt cacttttatc
      241 attgtgatat gaccacttcg tcattaatct catgagcgtt taaaatacta ccgccaaatc
      301 acgcaacgaa ctttgctgga ttcagtttat gctgctgtaa atccgctcgc agtaaacctt
      361 tcagacgcac cgtgatgtta tcagtggttc tttaagcgtt ttgctggtgt actcactaca
      421 aacgaattgc gaatcgaatc aatgtgaaac ggaaaggtac aatctccctt tttttagtgt
      481 taacatcgtt caaccgggtg gaaggtgaga ggttaatgtc tttcatttgt gtacctgatt
      541 gcgaaccaat ttagcatttg ttggctagat ggtttcgcaa tgaactgtta ataaacaaat
      601 ttttctttgt atgtgatctt tcgtgtgggt caccactgca aataaggata taaaatgcct
      661 gttataactc ttcctgatgg cagccaacgc cattacgacc acgctgtaag ccccatggat
      721 gttgcgctgg acattggtcc aggtctggcg aaagcctgta tcgcagggcg cgttaatggc
      781 gaactggttg atgcttgcga tctgattgaa aacgacgcac aactgtcgat cattaccgcc
      841 aaagacgaag aaggtctgga gatcattcgt cactcctgtg cgcacctgtt agggcatgcg
      901 attaaacaac tttggccgca taccaaaatg gcaatcggcc cggttattga caacggtttt
      961 tattacgacg ttgatcttga ccgcacgtta acccaggaag atgtcgaagc actcgagaag
     1021 cggatgcatg agcttgctga gaaaaactac gacgtcatta agaagaaagt cagctggcac
     1081 gaagcgcgtg aaactttcgc caaccgtggg gagagctaca aagtttccat tcttgacgaa
     1141 aacatcgccc atgatgacaa gccaggtctg tacttccatg aagaatatgt cgatatgtgc
     1201 cgcggtccgc acgtaccgaa catgcgtttc tgccatcatt tcaaactaat gaaaacggca
     1261 ggggcttact ggcgtggcga cagcaacaac aaaatgttgc aacgtattta cggtacggcg
     1321 tgggcagaca aaaaagcact taacgcttac ctgcagcgcc tggaagaagc cgcgaaacgc
     1381 gaccaccgta aaatcggtaa acagctcgac ctgtaccata tgcaggaaga agcgccgggt
     1441 atggtattct ggcacaacga cggctggacc atcttccgtg aactggaagt gtttgttcgt
     1501 tctaaactga aagagtacca gtatcaggaa gttaaaggtc cgttcatgat ggaccgtgtc
     1561 ctgtgggaaa aaaccggtca ctgggacaac tacaaagatg caatgttcac cacatcttct
     1621 gagaaccgtg aatactgcat taagccgatg aactgcccgg gtcacgtaca aattttcaac
     1681 caggggctga agtcttatcg cgatctgccg ctgcgtatgg ccgagtttgg tagctgccac
     1741 cgtaacgagc cgtcaggttc gctgcatggc ctgatgcgcg tgcgtggatt tacccaggat
     1801 gacgcgcata tcttctgtac tgaagaacaa attcgcgatg aagttaacgg atgtatccgt
     1861 ttagtctatg atatgtacag cacttttggc ttcgagaaga tcgtcgtcaa actctccact
     1921 cgtcctgaaa aacgtattgg cagcgacgaa atgtgggatc gtgctgaggc ggacctggcg
     1981 gttgcgctgg aagaaaacaa catcccgttt gaatatcaac tgggtgaagg cgctttctac
     2041 ggtccgaaaa ttgaatttac cctgtatgac tgcctcgatc gtgcatggca gtgcggtaca
     2101 gtacagctgg acttctcctt gccgtctcgt ctgagcgcct cctatgtggg cgaagacaac
     2161 gagcgtaagg taccggtaat gattcaccgc gcaattctgg ggtcgatgga gcgtttcatc
     2221 ggtatcctga ccgaagaatt cgctggtttc ttcccgacct ggcttgcgcc agttcaggtt
     2281 gttatcatga atattaccga ttcacagtct gaatacgtta acgaattgac gcaaaaacta
     2341 tcaaatgcgg gcattcgtgt taaagcagac ttgagaaatg agaagattgg ctttaaaatc
     2401 cgcgagcaca ctttgcgtcg cgtcccatat atgctggtct gtggtgataa agaggtggaa
     2461 tcaggcaaag ttgccgttcg cacccgccgt ggtaaagacc tgggaagcat ggacgtaaat
     2521 gaagtgatcg agaagctgca acaagagatt cgcagccgca gtcttaaaca attggaggaa
     2581 taaggtatta aaggcggaaa acgagttcaa acggcgcgcc ctaaccgtat caatggcgaa
     2641 attcgcgccc aggaagttcg cttaacaggt ctggaaggcg agcagcttgg tattgtgagt
     2701 ctgagagaag ctctggagaa agcagaagaa gccggagtag acttagtcga gatcagccct
     2761 aacgccgagc cgccggtttg tcgtataatg gattacggca aattcctcta tgaaaagagc
     2821 aagtcttcta aggaacagaa gaaaaagcaa aaagttatcc aggttaagga aattaaattc
     2881 cgtcctagta cagatgaagg cgactatcag gtaaaactcc gcagcctgat tcgctttctc
     2941 gaagagggtg ataaagccaa aatcacgctg cgtttccgcg gtcgtgagat ggcgcaccag
     3001 caaatcggta tggaagtgct taatcgcgtg aaagacgatt tgcaagaact ggcagtagtc
     3061 gaatccttcc caacgaagat cgaaggccgc cagatgatca tggtgctcgc tcctaagaag
     3121 aaacagtaag gccttcaagt agcactatct gtggagcctt tgggcttcac aggcattgtt
     3181 cgcctttgtt tcgtttatta acaatgcgaa gtggaagtta ttaaaatgcc aaaaattaag
     3241 accgtacgcg gtgctgctaa gcgcttcaaa aaaaccggta aaggtggttt taagcacaag
     3301 cacgctaacc tgcgtcacat tctgactaaa aaagcgacca aacgtaaacg tcacctgcgt
     3361 ccgaaagcca tggtttccaa aggcgatctg ggcctggtaa tcgcgtgcct gccgtacgca
     3421 taagccgtta acgtttttaa ctttttaatt agaatataga tacaggagag cacatatggc
     3481 tcgcgtaaaa cgtggtgtta ttgcacgcgc acgtcacaag aaaattttga aacaagctaa
     3541 aggctactac ggtgcgcgtt ctcgcgtata ccgcgttgcc ttccaggctg ttatcaaagc
     3601 tggtcagtat gcttaccgtg accgtcgtca acgtaagcgt cagttccgtc aactgtggat
     3661 tgcgcgtatc aacgcagcag cacgtcagaa cggtatttct tacagcaaat tcatcaatgg
     3721 cctgaaaaaa gcctctgttg aaatcgaccg taagatcctg gctgatatcg cagtattcga
     3781 caaagtagcg ttcaccactc tggttgaaaa agcgaaagca gctctggcat aagccagttg
     3841 aaagagggag ctagtctccc tcttttcgtt tcaacgccat caaaacattg acttttatcg
     3901 ccgtagcctt ttcaataaag gtcttttgaa gagtaccaaa aggtaacgca agcaatgaat
     3961 gctgctattt tccgcttctt tttttacttt agcacctgaa tccaggaggc tagcgcgtga
     4021 gaagagaaac ggaaaacagc gcctgaaagc ctcccagtgg aggctttttt tgtatgcgcg
     4081 ttttgaaaaa taaaaagcca gtctatcagg cgtaattgtc gcagccagtc tggacacggc
     4141 cagtgcggag aaaccggagc gtacttaagt acgtgaggat ttcgagcact gcccgggacc
     4201 aaaatggcaa gtaaaatagc ctgatgggat aggctctaag tccaacgaac cagtgtcacc
     4261 actgacacaa tgaggaaaac catgtcacat ctcgcagaac tggttgccag tgcgaaggcg
     4321 gccattagcc aggcgtcaga tgttgccgcg ttagataatg tgcgcgtcga atatttgggt
     4381 aaaaaagggc acttaaccct tcagatgacg accctgcgtg agctgccgcc agaagagcgt
     4441 ccggcagctg gtgcggttat caacgaagcg aaagagcagg ttcagcaggc gctgaatgcg
     4501 cgtaaagcgg aactggaaag cgctgcactg aatgcgcgtc tggcggcgga aacgattgat
     4561 gtctctctgc caggtcgtcg cattgaaaac ggcggtctgc atccggttac ccgtaccatc
     4621 gaccgtatcg aaagtttctt cggtgagctt ggctttaccg tggcaaccgg gccggaaatc
     4681 gaagacgatt atcataactt cgatgctctg aacattcctg gtcaccaccc ggcgcgcgct
     4741 gaccacgaca ctttctggtt tgacgctacc cgcctgctgc gtacccagac ctctggcgta
     4801 cagatccgca ccatgaaagc ccagcagcca ccgattcgta tcatcgcgcc tggccgtgtt
     4861 tatcgtaacg actacgacca gactcacacg ccgatgttcc atcagatgga aggtctgatt
     4921 gttgatacca acatcagctt taccaacctg aaaggcacgc tgcacgactt cctgcgtaac
     4981 ttctttgagg aagatttgca gattcgcttc cgtccttcct acttcccgtt taccgagcct
     5041 tctgcagaag ttgacgtcat gggtaaaaac ggtaaatggc tggaagtgct gggctgcggg
     5101 atggtgcatc cgaacgtgct gcgtaacgtt agcatcgacc cggaagttta ctctggtttc
     5161 gccttcggga tggggatgga gcgtctgact atgttgcgtt acggcgtcac cgacctgcgt
     5221 tcattcttcg aaaacgatct gcgtttcctc aaacagttta aataaggcag gaatagatta
     5281 tgaaattcag tgaactgtgg ttacgcgaat gggtgaaccc ggcgatcgat agcgatacgc
     5341 tggcgaatca aatcactatg gcgggcctgg aagttgacgg tgtagaaccg gttgccggta
     5401 gcttccacgg cgtggtcgtt ggtgaagtgg ttgagtgtgc gcagcatccg aacgctgaca
     5461 aactgcgtgt gacaaaagtg aatgtcggcg gcgatcgcct gctggacatc gtctgcggtg
     5521 cgccaaactg ccgtcagggc ctgcgtgtgg cggtagctac cattggtgct gttctgccgg
     5581 gtgatttcaa aattaaagcg gcgaaactgc gtggcgaacc gtctgaaggg atgctgtgct
     5641 ccttttctga gctgggaatt tctgacgatc acagtggcat tatcgaactg cctgcggatg
     5701 cgccgattgg caccgacatc cgcgaatacc tgaaactcga tgataacacc atcgaaatca
     5761 gcgtgacacc aaaccgtgcc gactgcttag gtatcattgg tgttgcgcgt gacgttgccg
     5821 tgctgaatca gctgccgctg gttgaaccgg aaatcgttcc ggttggtgcg actatcgacg
     5881 acacgttgcc gattacagtc gaagcgccgg aagcctgccc gcgctatctt ggccgtgtgg
     5941 taaaaggcat taacgttaaa gcgccaactc cgctgtggat gaaagaaaaa ctgcgtcgtt
     6001 gcgggatccg ttctatcgat gcagttgttg acgtcaccaa ctatgtactg ctcgaactgg
     6061 gccagccgat gcacgctttc gataaagatc gcattgaagg cggcattgtg gtgcggatgg
     6121 caaaagaggg cgaaacgctg gtgctgcttg atggcactga agcgaagctg aatgctgaca
     6181 ctctggtcat cgccgaccac aacaaggcgc tggcgatggg cggcatcttt ggtggcgaac
     6241 actctggcgt gaatgacgaa acgcaaaacg tcttgctgga atgcgctttc ttcagcccgc
     6301 tgtctatcac cggtcgtgct cgtcgtcatg gcctgcatac tgatgcgtct caccgttatg
     6361 agcgtggcgt tgatccggta ctgcagcaca aagcgatgga acgtgcgacc cgtctgctga
     6421 tcgacatctg cggtggtgag gctggcccgg taattgatat caccaacgaa gcaacgctgc
     6481 cgaagcgtgc aaccatcact ctacgtcgta gcaaactgga tcgcctgata ggccatcata
     6541 ttgctgatga gcaggtaact gacattctgc gtcgtctcgg ctgcgaagtg accgaaggca
     6601 aagacgaatg gcaggcagtt gcgccgagct ggcgtttcga tatggagatt gaagaagatc
     6661 tggtcgaaga agtcgcgcgt gtttacggct acaacaacat cccggatgag ccggtacagg
     6721 caagcctgat tatgggtact caccgtgaag ctgacctgtc gctcaagcgc gtgaaaacgc
     6781 tgctcaacga caaaggctat caggaagtga tcacctacag cttcgttgat ccgaaagtgc
     6841 agcagatgat ccatccaggc gttgaagcct tactgctggc aagcccgatc tctgttgaaa
     6901 tgtcagcaat gcgtctttct ctgtggaccg gcctgctggc aaccgtggtg tacaaccaga
     6961 accgtcagca gaaccgtgtg cgcattttcg aaagcggtct gcgtttcgta ccagatactc
     7021 aggcaccgtt gggcattcgt caggatctga tgttagccgg tgtgatttgc ggtaaccgtt
     7081 acgaagagca ctggaacctg gcaaaagaga ccgttgattt ctatgatttg aaaggcgacc
     7141 ttgaatccgt tctcgacctg accggtaaac tgaatgaggt tgagttccgt gcagaagcga
     7201 atccggcact gcatccgggg caatccgcag cgatttatct gaaaggtgaa cgtattggtt
     7261 ttgttggagt tgttcatcct gaactggaac gtaaactgga tcttaacggt cgcactctgg
     7321 tgttcgaact ggagtggaac aagctcgcag accgcgtggt gcctcaggcg cgcgagattt
     7381 ctcgcttccc ggcgaaccgt cgtgacatcg cggtggtggt cgcagaaaac gttcccgcag
     7441 cggatatttt atccgaatgt aagaaagttg gcgtaaatca ggtagttggc gtaaacttat
     7501 ttgacgtgta ccgcggtaag ggtgttgcgg aggggtataa gagcctcgcc ataagcctga
     7561 tcctgcaaga taccagccgt acactcgaag aagaggagat tgccgctacc gtcgccaaat
     7621 gtgtagaggc attaaaagag cgattccagg catcattgag ggattgaacc tatggcgctt
     7681 acaaaagctg aaatgtcaga atatctgttt gataagcttg ggcttagcaa gcgggatgcc
     7741 aaagaactgg ttgaactgtt tttcgaagag atccgtcgcg ctctggaaaa cggcgaacag
     7801 gtgaaactct ctggttttgg taacttcgat ctgcgtgata agaatcaacg cccgggacgt
     7861 aacccgaaaa cgggcgagga tattcccatt acagcacggc gcgtggtgac cttcagaccc
     7921 gggcagaagt taaaaagccg ggtcgaaaac gcttcgccca aagacgagta atctgatcta
     7981 actaaaaagg ccgctatgcg gccttttttc ttttcactgt cgaagagtca ccgtaaaatc
     8041 aacgccatga cacttcagca gaacggatac catgctgaca cttgcccgcc aacaacagcg
     8101 acaaaatatt cgctggttat tatgcctgtc agttttgatg ctgctggcgc ttctcttaag
     8161 cctttgcgcc ggtgaacaat ggatttcgcc aggtgactgg tttactcctc gtggcgaact
     8221 gttcgtctgg caaattcgcc tgccacgtac gctggctgta ttgctggttg gtgcggcgct
     8281 ggctatatcc ggcgctgtaa tgcaggcgtt gtttgaaaat cctctggcag aacctggact
     8341 acttggcgtc tctaacggcg caggcgtggg gcttatcgcc gcggtattgc ttgggcaagg
     8401 gcaactcccc aactgggcgc tagggctgtg tgcgattgct ggcgcgctta tcatcacttt
     8461 aatactctta cgtttcgccc gtcgtcatct ttcgaccagt cggttattgc tggctggcgt
     8521 tgcattaggg attatctgca gcgcactaat gacgtgggct atctactttt ccacctctgt
     8581 tgatttacgt cagctgatgt actggatgat gggcggtttt ggcggcgtag actggcggca
     8641 aagctggctg atgctggcat tgatccccgt gttgttgtgg atctgttgtc agtccaggcc
     8701 gatgaatatg ttagcgcttg gcgagatctc ggcgcggcaa ctgggtttac ccatgtggtt
     8761 ctggcgcaat gtgctggtgg cagcgaccgg ctggatggtt ggcgtcagtg tggcgctggc
     8821 gggtgctatc ggctttattg gtctggtgat cccacatatt cttcggttgt gtggtttaac
     8881 cgatcatcgc gtattacttc ccggctgcgc gctggcaggg gcgagcgcat tgctgctggc
     8941 cgatattgta gcgcgcctgg cattagctgc cgcagagctg cctattggcg tggtcaccgc
     9001 aacgttgggt gcgccggtgt ttatctggtt attgttaaaa gcaggacgtt agccgcaaaa
     9061 agacggtcta tgattaaaag ctaagatttt tactgaccac acctaggaga aacgatgcaa
     9121 gattccattc tgacgaccgt agtgaaagat atcgacggtg aagtgaccac gctggagaag
     9181 tacgccggta atgtgctgtt aattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa
     9241 tatgagcagt tggagaatat tcaaaaagcc tgggccgatc gaggttttgt ggtgctggga
     9301 ttcccgtgca accagtttct ggaacaagaa ccgggcagcg atgaagagat taaaacttac
     9361 tgtaccacca catggggggt gacgttcccg atgttcagta agattgaagt taatggcgaa
     9421 ggacgccatc cgctgtatca aaaattgatt gccgcagcgc cgaccacagt cgcgccggaa
     9481 gagagcggat tctatgcccg tatggtcagc aaaggccgtg caccgctgta cccggatgat
     9541 attttatgga attttgaaaa attcctggtt ggcagggacg gaaaagtcat ccagcgtttt
     9601 tccccggata tgacgccgga agatcccatt gtgatggaaa gcattaaact ggcgttggca
     9661 aaataatgtc tattgtgatg cagttacagg atgttgcgga atctacccgc ctggggccgc
     9721 tttcaggcga ggttcgggct ggggaaatcc tgcacctggt ggggccgaat ggcgcgggta
     9781 agagtacctt actggcgcga atggccggaa tgaccagcgg taagggaagc attcagttcg
     9841 cggggcaacc actggaagca tggtccgcaa caaaactcgc gctgcatcgc gcctatcttt
     9901 cacaacagca gacgccgccg tttgcaatgc cggtctggca ctacctgaca ctgcatcagc
     9961 acgataaaac gcgtaccgaa ctactgaatg atgtcgcagg ggcgctggct cttgatgaca
    10021 aactcggtcg tagcaccaat caactttccg gcggtgaatg gcaacgcgta cgtcttgctg
    10081 cggtggtgtt gcaaatcaca ccacaagcca atcccgcagg ccaattgctg cttcttgatg
    10141 agccgatgaa cagtcttgat gttgcgcaac aaagtgcgtt agacaaaatt ctgagcgcgc
    10201 tgtgtcagca aggactggcg attgtgatga gcagtcacga tctcaaccac acgttgcgtc
    10261 atgcgcatcg ggcgtggttg ctaaaaggtg gaaaaatgct ggccagtgga cgcagagaag
    10321 aggtgctcac gccgccaaat ctggcgcagg cctatgggat gaattttcgc cgtctggata
    10381 tcgaaggtca cagaatgctg atttcgacca tctgatttca ttagtatccc gaaaatcggg
    10441 taattacgac gctaaattaa tgcaagaata aaaaacagag gaaccgtacg gaatgcgttt
    10501 ctgccttatt ttgatcacag cactgtttct ggccgggtgt agccaccata aagcaccgcc
    10561 gccaaatgcc agactttctg attcgattac cgttattgcc ggtttgaacg accagctaca
    10621 aagctggcat ggcacgccgt atcgttatgg tggcatgacg cggcgcggtg tggactgttc
    10681 gggatttgtg gttgtgacga tgcgcgatcg tttcgatttg catctgcccc gtgaaaccaa
    10741 acaacaagcc tctattggca cgcaaattga taaagacgag ttgctgcctg gtgacctggt
    10801 ctttttcaaa acgggttccg gacaaaatgg tttgcatgta ggtatttacg ataccaacaa
    10861 ccaatttatc cacgcctcta ccagcaaggg agtgatgcgt tcctcacttg ataatgtcta
    10921 ttggcagaaa aatttctggc aggcgagacg aatctagtat gtcgaaaaat tgacggcgat
    10981 gatacatcgc cgtcaattta ctcctgaata cagtagtatt ttgcagacaa aaattccgta
    11041 aatggattat tcgtttataa tcaggataat ctaaaaaaag gccccctgaa aaagtggtta
    11101 acatggaaca aatgaaagtt actccttctg taattgttat gtcttatggc ctcatccctt
    11161 ttagccggat actgaaaaac atccttcgag agggacggtt accatgaaga tttttttgga
    11221 gaatctttat cattctgatt gctatttcct gccgataaga gataaccagc aactccttgt
    11281 cggcgttgag ttaattaccc atttctccag tgaagatggc acggttcgta tcccgacgag
    11341 tcgggttata gcgcaactta ccgaggagca acattggcaa ttattttcag agcagttgga
    11401 attactaaaa tcatgccaac acttttttat tcaacataaa ctctttgcct ggcttaattt
    11461 aacgccacaa gtggctacat tattattaga cagggataat tacgcaggcg aactattaaa
    11521 atatccgttt attgagctgt tgattaatga gaattacccg catctcaatg aaggaaaaga
    11581 taatcgcgat cttttgtcct tatcgcaaat gtatcccctt gtgttaggca atttgggagc
    11641 aggtaatagc acaatgaaag cggtttttga tggtcttttc acccgcgtaa tgctggagaa
    11701 aggttttatt cagcaacaaa tcacgcatcg ctccttcgaa ccgtttattc gtgcgatcca
    11761 ggcgcaaatt tccccttgtt gcaattgcat catcgcgggg ggcattgata cgccagaaat
    11821 attggcacag attaccccct tcgatttcca tgctctccag ggatgcctgt ggcctgccgt
    11881 tcctataaat cagataacga cgttggttca gcgataaccc tcctgtttgc tggtgtttaa
    11941 gacgagagta accgtctaca ctatcaaaca gataggatct atgaccctgt cttttattac
    12001 ccgctggcgc gatgaattgc cagaaaccta tacagcactt tcccctacgc ctttaaataa
    12061 tgcccggctg atttggcata ataccgaact cgctaacacg ctgagtattc catcgtcgct
    12121 gtttaaaaat gccgcaggtg tctggggcgg cgaaacccta ctgcctggta tgtcaccact
    12181 ggcacaggtt tacagtggtc atcagttcgt cgtctgggcg ggccaactgg gtgatgggcg
    12241 cggcatttta cttggcgaac aactgcttgc tgatggcact acaatggact ggcatctgaa
    12301 aggtgctggc ctgacgcctt attcgcgaat gggtgatgga cgggcggttt tacgttcgac
    12361 gatacgagaa agtctcgcca gtgaggcgat gcattatctg ggcattccga cgacccgcgc
    12421 gttaagtatc gtcaccagcg attcgccagt gtatcgggaa acggtggagc caggcgcgat
    12481 gctgatgcgt gtggcaccaa gtcatctgcg ctttggtcat ttcgaacatt tttactatcg
    12541 ccgcgagccg gaaaaggttc gtcagttggc tgactttgcc attcgtcatt actggtcaca
    12601 tcttgaagac gatgaggaca aataccgtct ctggtttaac gatgttgtcg cacgtaccgc
    12661 gtcgttaatt gcccaatggc agacggtcgg ctttgcacat ggggtgatga ataccgacaa
    12721 catgtcgctg ctgggactga cgcttgatta cgggccgttt ggttttcttg atgattacga
    12781 acccggtttt atttgtaatc actcggatca tcaagggcgt tatagctttg ataatcaacc
    12841 tgctgtcgcg ttgtggaatt tacagcgtct ggcgcagaca ttgtcaccat ttgttgccgt
    12901 agatgccctg aatgaggccc tggacagcta tcagcaggtt ttgttgacgc attatggaca
    12961 acggatgcgg cagaaactgg gcttcatgac ggagcaaaaa gaggataacg cgctactgaa
    13021 tgaattattc agtctgatgg cgcgagagcg cagcgattat acccgcacat tccgcatgct
    13081 gagtctgacc gagcagcaca gcgcggcgtc accgctacgt gaggagttta ttgatcgtgc
    13141 ggcatttgat gactggtttg cccgttatcg ggggcgtttg caacaagacg aggttagcga
    13201 tagtgagcgt cagcaactga tgcaaagcgt taacccagct ctggtattgc gcaactggtt
    13261 ggcgcaacgg gcgattgaag cggcagaaaa gggtgatatg atggaattgc accgcctgca
    13321 tgaggcgttg cgaaatcctt tcagcgacag agatgatgac tatgtcagtc gtccacctga
    13381 ctggggtaaa cggctggaag tcagctgctc gagttaaagg ctacttagtt aacagcagct
    13441 tgccagcctg agttttacgg agcagatatt cttgcccgtc atgatcgata atcagtttgc
    13501 catccggacc taacagtgtc tggctggtaa ttcgccggat gggctgcggg gaagcggtct
    13561 tgtgattggc atccgtttca ggcgtgagtt ttctgctatc cgtataacgc ataaaaatta
    13621 ataataattg atataacaat gattcctatt atcaatatta tggcagacca gagcaagcag
    13681 taatgtgttg agaagacaac aagttgcgca gaaatgccca tcccatcagg aatgggcacg
    13741 cattcagaag cgggtatcta ccgcaaaggc gagtttttcg accaggcgtt cggtatcttc
    13801 ccagcccaga cacgggtcgg taatggattg accgtaagtg agcggctgac cgccgacgat
    13861 tttttgcgtt ccttcgcgca ggaaactttc cgccataatt ccagcaatct ccgtagagcc
    13921 attgcggatt tgctgacaaa tatcctcaca aacttctaac tggcgacggt gctgcttctg
    13981 gcagttaccg tggctgaaat ccaccaccag atgttcaggt aaatcaaact cgtgcagcgt
    14041 atcgcaggct gcggcgatat catcggcatg ataattcggt tttttgccgc cacgcataat
    14101 aatgtggcca tacgggttgc cgctggtctg atagatggtc atctgaccat ttttgtctgg
    14161 cgagaggaac atatggctgg cgcgggctgc gcggatagca tccacggcaa tccgcgtgtt
    14221 gccatcggta ccatttttaa aacctaccgg acaggagagt gctgaagcca tttcgcggtg
    14281 gatctgactt tcggtagtac gagcgccaat cgcgccccaa ctgattaaat cagcaataaa
    14341 ctgaccggtt accatatcga ggaactcagt cgcggtaggg acacccagct catttacctg
    14401 taaaagtaat ttgcgcgcca gctccagacc gtgatttacc cgatagctgc cgtttaaatc
    14461 cggatcggag attagccctt tccagccgac aacagttcgt ggtttttcaa aataggtgcg
    14521 cattacgatt tccagccgtg actggtactg gttgcgcagc gactgcagac gggtggcgta
    14581 ctccattgca gcggtgagat cgtggatcga gcaggggcca ataatgacca acagtcgctt
    14641 atcttcacca ttcagtattt tttcaattct gcggcgggag tcggtgacat gggtggcgac
    14701 gccaggcgtt acgggatacc gtagcgcgag ttcggcgggc gttaccaggc tctcaatacg
    14761 cgcagtacgg agttcgtcag ttctgttcat tacaagtctc agaaatttgt tgcttctacg
    14821 gcaggactac cggaaagtga tgcgcatcac gataaaccaa tcgcagctga tttcaagtgc
    14881 gaggtgtgtc acacctcgcc gggtggttag catgataaca aaaaaacaaa ccaatgcact
    14941 agttctctag tacattcggc gactaaggcc catgatatcg aggatcttgg tggcaatctc
    15001 ttctaccgaa taattggtac tgttaatcca cgggatctga tttttacggt acaaggcttc
    15061 cacttccgcg acttccatcc tgcactgacg aagcgaggca tagcgactgt tctcccgacg
    15121 ttcctcgcga atcgccgcca gacgttccgg gtcgatagtc aggccgaaca atttatgctg
    15181 aagcggtttg agcgacgcgg gtagcaccag attatccata tcgtcggcaa taaaggggta
    15241 gtttgccgcg cggataccaa attgcattgc cagatacaga ctggtggggg ttttaccaca
    15301 gcgagaaaca ccgagcagga tcacctgagc ctgatccaga ttgcgcaacg aaatgccgtc
    15361 atcgtgggcg agggtgtaat caatcgccgc aatgcgcgca tcatatttat tgagattatt
    15421 agggttaagg ccatgggtac gatgagcaat cggcgttgga tccagtttca tctcttgttg
    15481 tagcggggca accagcgcct gaacgatatc ctggcaaaag ccttcacttt gcaagatgat
    15541 ggcgcgaatc tccggcaaca cgatggagta gaagaccagc gggcgcacgc ctgtctggtg
    15601 ataaattgcg tcaatctgat ccttcactgc ccgtgcacgg ctctcatttt cgacaaacgg
    15661 cagcgtgatg ctgctgatag tgacgggaaa ttgtgacatt actgcgtgtc ctaatacctc
    15721 cgcagttatt gccgtaccat cagaaatata aaaaacgtgg cgatcaacag cattatccat
    15781 tttgttcttc ccgtgaagca gacataattc tatgaaagca taaattaaaa gcgcacagaa
    15841 gcgtaggacg taatgtctgg tttataaaat gaaccttcat tttatttttt ataaaaacag
    15901 catttcattt ttatggtttc gtttataccg atggtttatg tggaaattgt cgaatagagc
    15961 aggtttgcgc aacgctggga tcagtcttaa aaagtaaaaa aatatatttg cttgaacgat
    16021 tcaccgtttt tttcatccgg ttaaatatgc aaagataaat gcgcagaaat gtgtttctca
    16081 aaccgttcat ttatcacaaa aggattgttc gatgtccaac aatggctcgt caccgctggt
    16141 gctttggtat aaccaactcg gcatgaatga tgtagacagg gttgggggca aaaatgcctc
    16201 cctgggtgaa atgattacta atctttccgg aatgggtgtt tccgttccga atggtttcgc
    16261 cacaaccgcc gacgcgttta accagtttct ggaccaaagc ggcgtaaacc agcgcattta
    16321 tgaactgctg gataaaacgg atattgacga tgttactcag cttgcgaaag cgggcgcgca
    16381 aatccgccag tggattatcg acactccctt ccagcctgag ctggaaaacg ccatacgcga
    16441 agcctatgca cagctttccg ccgatgacga aaacgcctct tttgcggtac gctcctccgc
    16501 caccgcagaa gacatgccgg acgcttcctt tgccggtcag caggaaacct tcctcaacgt
    16561 tcagggtttt gacgccgttc tcgtggcagt gaaacatgta tttgcttctc tgtttaacga
    16621 tcgcgccatc tcttatcgtg tgcaccaggg ttacgatcac cgtggcgtgg cgctctccgc
    16681 cggtgttcag cggatggtgc gctccgacct cgcatcttct ggcgtgatgt tctccattga
    16741 taccgaatcc ggctttgacc aggtggtgtt tatcacttcc gcatggggcc ttggggaaat
    16801 ggtcgtgcag ggtgcggtta acccggatga gttttacgtg cataaaccga cactggcggc
    16861 gaatcgcccg gctatcgtgc gccgcaccat ggggtcgaaa aaaatccgca tggtttacgc
    16921 gccgacccag gagcacggca agcaggttaa aatcgaagac gtaccgcagg aacagcgtga
    16981 catcttctcg ctgaccaacg aagaagtgca ggaactggca aaacaggccg tacaaattga
    17041 gaaacactac ggtcgcccaa tggatattga gtgggcgaaa gatggccaca ccggcaaact
    17101 gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc ggtcaggtca tggagcgtta
    17161 tacgctgcat tcacagggta agattatcgc cgaaggccgt gctatcggtc atcgcatcgg
    17221 cgcgggtccg gtgaaagtca tccatgacat cagcgaaatg aaccgcatcg aacctggcga
    17281 cgtgctggtt actgacatga ccgacccgga ctgggaaccg atcatgaaga aagcatctgc
    17341 catcgtcacc aaccgtggcg gtcgtacctg tcacgcggcg atcatcgctc gtgaactggg
    17401 cattccggcg gtagtgggct gtggagatgc aacagaacgg atgaaagacg gtgagaacgt
    17461 cactgtttct tgtgccgaag gtgataccgg ttacgtctat gcggagttgc tggaatttag
    17521 cgtgaaaagc tccagcgtag aaacgatgcc ggatctgccg ttgaaagtga tgatgaacgt
    17581 cggtaacccg gaccgtgctt tcgacttcgc ctgcctgccg agcgaaggcg tgggccttgc
    17641 gcgtctggaa tttatcatca accgtatgat tggcgtccac ccacgcgcac tgcttgagtt
    17701 tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag atgatgaaag gttttgattc
    17761 tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc gcgacgctgg gtgccgcgtt
    17821 ttatccgaag cgcgtcattg tccgtctctc tgattttaaa tcgaacgaat atgccaacct
    17881 ggtcggtggt gagcgttacg agccagatga agagaacccg atgctcggct tccgtggtgc
    17941 gggccgctat gtttccgaca gcttccgcga ctgcttcgca ctggagtgtg aagcagtgaa
    18001 acgtgtgcgc aacgacatgg ggctgaccaa cgttgagatc atgatcccgt tcgtgcgtac
    18061 cgtagatcag gcgaaagcgg tggttgaaga actggcgcgt caggggctga aacgtggcga
    18121 gaacgggctg aaaatcatca tgatgtgtga aatcccgtcc aacgccttgc tggccgagca
    18181 gttcctcgaa tatttcgacg gcttctcaat tggctcaaac gatatgacgc agctggcgct
    18241 cggtctggac cgtgactccg gcgtggtgtc tgaattgttc gatgagcgca acgatgcggt
    18301 gaaagcactg ctgtcgatgg ctatccgtgc cgcgaagaaa cagggcaaat atgtcgggat
    18361 ttgcggtcag ggtccgtccg accacgaaga ctttgccgca tggttgatgg aagaggggat
    18421 cgatagcctg tctctgaacc cggacaccgt ggtgcaaacc tggttaagcc tggctgaact
    18481 gaagaaataa aataaatccc cggcggcgtt cagtcgccgg ggttatgtga tccccgaaga
    18541 tgaaacttat tcaatctctt cacagacatc ctgcgttaat cgccgtataa tatcttttct
    18601 taacaaaaac ttttgtattt tatctgaggc agtgcgcggt agtttttcga ttaccacgat
    18661 atgttcagga tatttatatt ttgcgacccg tttacggcta aaaaaagccg ccatctcttc
    18721 cagcgataat gaatgatgcg gcgctttcag cacgacataa gcgcatgatc gttcacctaa
    18781 acgttcatcg ggcattgcaa ccacacaggc atcgtgaatt ttaggatgct gcaataaaat
    18841 atcttccact tcacggctgc taatattttc gccgccgcgg acaataatat cttttttgcg
    18901 tccggtaatt tttatatagc cagcctcatc catacggcag agatcgccgc tgtaatacca
    18961 gccttcttca tccagcgcac gggcggttaa ttcaggttca tcaaaatacc ccataaacac
    19021 attggggcca cgcgatgctt cttcaccttc gcaacctggc ggtaaggtct tgcgtgcgtc
    19081 atcgaccact ttaatctcta cacctgcggc agcgtaacca tcggtatgca taaagcgcga
    19141 caaagaatta tcgagattca ccaccgcatg cggcgaactt tctgtggaac cataaacact
    19201 taataattta atgctgcgct gctggcattc gcgcgccact tttttgggga ttgtggtacc
    19261 gccgcaaaga aagaagcgca gcgctgaaag gtctgcgggt tgtttctcta gtaaattcaa
    19321 aagatcatag acaaacggcg ttgcgcctag catacaggtg caacgctgct gctcaagcag
    19381 cgcgagacac gcatcaggag tgaaaatatc taacaacacg ctgcgagcgc caattaagaa
    19441 tggtgccgtt acgccatgca gaaagcccgt tgcgtgacca agtggcgcag gcatcataaa
    19501 gacatccagc caggtcagat tcagccgcgc gcaataagcc cgctcactgg cgagaatatt
    19561 gttatgcgtt agcatcacgc cctttggcag accctcggtt ccggaggtaa acagcaccgc
    19621 agctaattca tcgccgtggg tcgttatcgc cgtggtcagt gggatattgt cggcgataat
    19681 ctgacttaat gagagggaag aggtggcggg agccagtttg tccacgccga caatttgttg
    19741 tagttgtgga agctgatttt gcagcggcag gattaaatct accggacgcg tttgtttaaa
    19801 caacgtcggt gcaaagaaca tttttgcctg acacttattg agcacccaca ccagttctgc
    19861 ttcccgccag gaaggcaaca gcggcacgga aaccgcaccg attttcaggc aggcaagata
    19921 gataacggta aattcacacc agccaggcag ttgaaatgcg atgcgatcgc ctgattcaat
    19981 acccttcgct aacatccagt ttgccagaca gctcgcggcg tgatcgagcg cgctataggt
    20041 gtacgatgca ccatgattat cgaccacggc aattttgtct ggcatcgcac gagcggtctg
    20101 ctgccagtaa tcggccagtg aagcatcgcc ccatagccct tgctgacgat acgccgcacg
    20161 acgttgttcg ttaaacgtta atgtcacttt cattttggac tctcgaaaca gaacatcagg
    20221 cccgagttgc gggcctgtgg gatgcatgat tagccgtagc gaaaatcaat accgaaggtg
    20281 cctgcgggat attgccactg ttcgagaata gtgttaccgc acagcacccg acaggtgccg
    20341 cactccagac aaccggcggg atcaaaatga atgtttccta cgtcatcctg cttataaagt
    20401 ccggcagggc aggctttcat taatttacgg aattcattga tatcgggatt ttccgtcaga
    20461 atgatatgcg ggtggccctc atcaacatgg aatttattga cgcctaattt gatgtcgacg
    20521 ttaaccgtag cgttctggct catagcgcgg ttgctccctt aatgccatct ttcagcaagt
    20581 tgatcagccc aattttcttc gcgtgtccca tgatcatttt gcgtaccggc tggtttggtt
    20641 tgccgtcaat ggtgaacatc tcgttcatga tgtcggctac cattcgtggg tattggctaa
    20701 acaggcgcgg gttttccatc agcgccggga tcttgcgaaa atgctgcata tcacgcatga
    20761 cgcagctttg ttccagctca cgtttgtatt gcgccagact gttggcggag aaatccgcgc
    20821 gttctttggc ggcgatcacc gttgtggcgg cagcctgagc cgatgcaatg gctaaatcca
    20881 tgccgcggac cgtaaaaccc aaattcaggc agaagcctgc ggcgtcacca acgatcatca
    20941 cgccctcgtt aaccagttgc ggcaccattg ccagaccgcc ttccggcacc atatgcgcgg
    21001 aatattcaag cagtttgccg ccgctaatca gcgggcgaat ggcggggtgt tgtttaaaat
    21061 cttccagcat ttgcggcacg cttttttgcg catgggcgat atcacccaat ccacaaacca
    21121 gccccaagga tatggaatcc ttgttggtat agagaaatcc cccgcccatc aggccgtcag
    21181 aaggggaacc ggcaaacagc caggcggcac cttcctcgcc cgtaatatta aagcgatcgt
    21241 tgatctgttc tggtgtgagg ccaataacct ctttaacacc aacagcgtaa tgatgcggat
    21301 cggaagcggg aaccattccc agcgagcggc caagcatcga gttaacgcca tcagccagaa
    21361 tcaccacatt cgcttcgaga atatcatccc cagcctgcac gccagtgacc ttgtttcctt
    21421 cacgaaccag cgcgtcgacg cgaacgcctg ggataaactg cgcgcccgcc tgctcggctt
    21481 gttccatcaa ccacgggtcc agacgattac gcaatacggt ataagatgcg tgttgaggaa
    21541 catctggttg ctcgcggtga aaatcgaggg taacggcgct ttcttcggtt aagaaggaga
    21601 ttttctcgcg tgtgaccttg cgttctaccg gcgctgatac tgcaaagcct ggaatgattg
    21661 cttcaagtgt gtgggcataa agacgcccgc cggtcatgtt tttacatccg gcactgtcgc
    21721 cgcgttctat caccaggaca tccagcccgg ctcgtgccat gaccagtgcg gcaacgctac
    21781 cagcaacgcc cgcaccgacc acaatggcat caaatttgtc atccgacatg cgttatgctc
    21841 ccttcctgct gcgtttacag cgggagatgt ggttgaaagg ggaattagcg gctcaactgg
    21901 ctaatcaggg cagggacgac tttgtagata tcgcccacca gaccgtagtc ggcatagttg
    21961 aagattggcg catttttatc tttattgatg gcgacaatca cttttacgcc gttgccgcca
    22021 accatatgtt ggatctgccc ggagatcccc agcgtcaggt agagatcgga tttcagcaac
    22081 acgccggaga caccgatata acgttcacgc tccatccagt tctcgccttc ggcaattgga
    22141 cgtgaacagc cgacttcagc attcagcacc gccgccagtt cgtggaccat tttcaggtca
    22201 tcctgcgccg ccagaccacg accgacgcca accacacgtt ttgctttgct caggtccacg
    22261 ctgcttgcgg ctttcgcacg gcgttcgcga cagagaattt catgacgcgg agcaacataa
    22321 ggtaccgttt ctgtcgggca ctgatgagag gtatcactgg tgcacggttc ctgaacaccg
    22381 ggtgcaaggg taatgatcgc cagcgggctg ttgatctttt cctgagcgaa cgctaacccg
    22441 ccatacatcc ggtgttcggc gcaaatgtga ccatcgacaa tatccaccgc cgtggcatcg
    22501 ttcaccagcg ccgcattcag ttgcacactt aaccgtgctg ccagagcttt accacgtttc
    22561 gtcgcggcca acagcaacat agcggggtgt ttatctttca gcagggcagc aatgctttcg
    22621 gcgtaatttt cagtgcgttg cagcgcgtcg ttttgctcaa gaacataaat acattttgga
    22681 ccataaggca taactgcctg cgcctggtcg gtattttgta caatggcata cacctgttgg
    22741 ccccattgct gagcgccgcc aaacagttca gcataacgtt caggattatc gctaaagacc
    22801 cagacgctgt ttaattgact catcgtttct tctccgtagg cttcagttca gggctttctt
    22861 cagatgttcc gccagctcgg caatggcctc cggcgaatcg ttatcgagaa tgatgtgctt
    22921 acgttctgtt tgcggcggta cgcgaatgcc gaccagttca gcaagtggcg cgctctggct
    22981 ccagtcaata tcacttgcct gccactgatt taccggtttt ttacccgcgc cgagaatggc
    23041 tttcatcgaa ggaatgcgtg gcacgttaat atcggaggtg acgcagagca cggctggaac
    23101 agagagttca ataacttcaa catcatcttc aagcgtgcgt tcaatcacca gtgtattgcc
    23161 ctgacgctga atagcactca ctgcattaat caccggaagt tgcagaattt ctccgaccag
    23221 caagccaacc tgctgggcat aaaggtcgcc ggaaccttca ccaaagatca gtaaatcgaa
    23281 gccgatcttt tcaattgctg ccgccagcgc ttttgcggta tcgagaggca gtgcatgttc
    23341 aagttgcgca tcctgcacca aatacaggct gtgcggcccg cgggatagca cgtctttgcg
    23401 cactttcgag ttctgcaaca atgagccgcc aacggtcagc gcggctatct catcgtcatc
    23461 tgttgcgagc tggcttgcag cttcaatggc attgagatcg aactggctga ttttggcgtc
    23521 ggcattgtcg aaattcaggg tgtattctgg agtgacaaca atgtcctgtt cttcaggcac
    23581 cagcttaaag caggttatta ttttcatgat ttctcctgtg agtcgcatac ggaaccatct
    23641 ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata gcggtgcggg ggggggggcg
    23701 acggagcaag ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatt gctaaaacaa
    23761 aagtgcaatt tgcggcaata tagttattcc atgaccgtac aatcttgcat tcagtgtaaa
    23821 taatcttgtt tagctctcgc taaacgcggt tgtataaccg caatacacgc cgtattttat
    23881 ccggcatata ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta tcaacgctgt
    23941 tttgataatg ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct ttcacgtttt
    24001 gcatttagcg atgatccaaa atgggagtct ggacatcacg ttcatgacaa tgaaaccgag
    24061 ctgatttacg tcaagaaagg ggttgcaagg tttaccatcg attcttcgtt atatgtcgca
    24121 catgcggatg acattgtggt gatagaacgc ggcaggctgc atgcggtggc ctctgacgtt
    24181 aacgatccgg caacgacgtg tacctgtgcg ctgtacggct ttcagtttca gggggctgag
    24241 gaaaatcagt tactgcaacc gcattcttgt cc
//
LOCUS       DAJTYH010000061        23820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTYH010000061 DAJTYH010000000
VERSION     DAJTYH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(42..914)
                     /gene="rluF"
                     /locus_tag="OO609_002406"
     CDS             complement(42..914)
                     /gene="rluF"
                     /locus_tag="OO609_002406"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6633792.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1262..2209
                     /locus_tag="OO609_002407"
     CDS             1262..2209
                     /locus_tag="OO609_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6633793.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2279..3085
                     /locus_tag="OO609_002408"
     CDS             2279..3085
                     /locus_tag="OO609_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6633794.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3095..3502
                     /locus_tag="OO609_002409"
     CDS             3095..3502
                     /locus_tag="OO609_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6633795.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3502..3996
                     /locus_tag="OO609_002410"
     CDS             3502..3996
                     /locus_tag="OO609_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6633796.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4062..4859
                     /locus_tag="OO609_002411"
     CDS             4062..4859
                     /locus_tag="OO609_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6633797.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4870..5658
                     /locus_tag="OO609_002412"
     CDS             4870..5658
                     /locus_tag="OO609_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6633798.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVSLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5710..6939
                     /gene="sorE"
                     /locus_tag="OO609_002413"
     CDS             5710..6939
                     /gene="sorE"
                     /locus_tag="OO609_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6633799.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7321..8010
                     /gene="pepE"
                     /locus_tag="OO609_002414"
     CDS             7321..8010
                     /gene="pepE"
                     /locus_tag="OO609_002414"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6633800.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8101..9732)
                     /locus_tag="OO609_002415"
     CDS             complement(8101..9732)
                     /locus_tag="OO609_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6633801.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9952..13635)
                     /gene="metH"
                     /locus_tag="OO609_002416"
     CDS             complement(9952..13635)
                     /gene="metH"
                     /locus_tag="OO609_002416"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6633802.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13835..14659
                     /gene="iclR"
                     /locus_tag="OO609_002417"
     CDS             13835..14659
                     /gene="iclR"
                     /locus_tag="OO609_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6633803.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            14976..17160
                     /gene="espL4"
                     /locus_tag="OO609_002418"
                     /pseudo
     CDS             14976..17160
                     /gene="espL4"
                     /locus_tag="OO609_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17129..18866)
                     /gene="aceK"
                     /locus_tag="OO609_002419"
                     /pseudo
     CDS             complement(17129..18866)
                     /gene="aceK"
                     /locus_tag="OO609_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19050..20354)
                     /gene="aceA"
                     /locus_tag="OO609_002420"
     CDS             complement(19050..20354)
                     /gene="aceA"
                     /locus_tag="OO609_002420"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6633804.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20384..21985)
                     /gene="aceB"
                     /locus_tag="OO609_002421"
                     /pseudo
     CDS             complement(20384..21985)
                     /gene="aceB"
                     /locus_tag="OO609_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22254..23183)
                     /gene="metA"
                     /locus_tag="OO609_002422"
     CDS             complement(22254..23183)
                     /gene="metA"
                     /locus_tag="OO609_002422"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6633805.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23340..23783
                     /locus_tag="OO609_002423"
     CDS             23340..23783
                     /locus_tag="OO609_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6633806.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5862 a         6074 c         6044 g         5840 t
ORIGIN      
        1 ccgagctctt tttggacgtt ttcatcagtc tgaggcgtta atcagcgtcc cttcttttta
       61 cgccccggcg aggtaaaacg cttaccgttg gacgccgggc gaccgccttt ttccgccgtt
      121 ttttccatct taacgactgg acgtttgatg cccgctgttt tcggtttggc cttcgcttta
      181 ggttttgcct cggaagagga attttcgatg agcttaaaga ggtcgattaa ctcatcgtcg
      241 gttaaatcgc gccactcccc cagcggaatg ccgcttaagc tgacgttcat aatgcgcgta
      301 cgttccagct ttttcacttc atagccgaaa tgctcgcaca tgcgccggat ctgacggttc
      361 agcccctgca ctagggtaat gcggaagaca aacggcgctt cttttttgac tttgcacttt
      421 ttcgtcaccg tcccgaggat cggcaccccc gcccccatac cacgaataaa ttcatcggta
      481 atcggtttat cgaccgtcac cagatactct ttctcatgat cgttacccgc acggaggatc
      541 ttattcacca gatcgccgtg attggtgagg aaaatcagcc cctgggagtc tttatccagg
      601 cggccaatcg ggaacacgcg tttgctgtgg ttaacgaaat cgacaatgtt atcgcgctcg
      661 ccatcttcgg tggtgctgac aataccgacg ggcttgttca gggcgataag taccaaatct
      721 tcggcttccc gaggctcaat caactgaccg tttactttca caatgtcgcc gggtttcacc
      781 tgatcgccaa tggtggctcg cttgccatta aggaacacat tgccttgctc gatatagcga
      841 tccgcttcgc ggcgtgagca aattccgctt tcgctgatgt atttatttaa acggactgat
      901 gagtcgggca gcataaattc tcctgtaaaa cgcggaatat accgtacctc ttagcgggga
      961 aaaacagggt tatgcggcaa cgtcgaaatt aaatgtgatc tgccacgcct tttatcacaa
     1021 aagtgtctcc ggaagtacga tgcaaatttg cctggcaaaa taaggataat caacatatta
     1081 cgaatcgctg cacacctgtg ctgatgacta ctacgcagag aaacaaatgt gcaagagaaa
     1141 gcgctatttc tccccatgca aagagtgaaa aatcgctttg aaaggccctc atgatggggt
     1201 tatatcagat caagcggtga gaaagcgtat ttgcacaaat gtgcagaacc aggaggcggc
     1261 aatggaaaac agtgacgata tccgtttgat tgtgaagatt gcccaactct attacgaaca
     1321 ggatatgacg caggcgcaaa tcgcgcgcga actgggtatt taccgcacca ccatcagccg
     1381 cttgcttaaa cgaggccgcg atcagggaat ggtcaccatc gccatcaact atgactacaa
     1441 cgaaaatctc tggctggagc agcaactgaa gcaaaagttt ggcctgaaag acgttgtggt
     1501 ggtgtcggga aatgatgagg atgaagagac tcaactggcg atgatggggt tacacggcgc
     1561 gcaactgctg gatcgcttgc tggagcctgg cgatattgtc ggtttttcct ggggtcgcgc
     1621 ggtgagcgca ctggttgaaa acttgccgca ggcggggcaa tcgcggcagt taatctgcgt
     1681 accaattatt ggcggaccgt ccggtaaact cgaaagccgc tatcatgtaa acacattaac
     1741 ctacagcgcg gcagcgaagc tgaaagggga atcgcatctc gcggattttc cggctctgct
     1801 ggataaccca ttaattcgta atgggatcat gcagtctcag cactttaaaa ccatctctgc
     1861 ctactgggat aatctggata tcgccctggt gggaattggc tcaccggcca ttcgcgacgg
     1921 cgctaactgg catgcgtttt atggtggtga agagagtgac gacctgaatg cccgccaggt
     1981 tgctggcgat atttgctcgc gcttttttga tattcacggc gcaatggttg aaacgaatat
     2041 gagcgaaaaa acgctctcta tcgaaatgaa taaattaaag caggcgcgat attccattgg
     2101 cattgccatg agtgaagaaa aatacagcgg aattattggt gcactgcgtg gaaaatatat
     2161 taattgtctg gtaacgaata gcagcacagc tgaactatta ctgaaataaa aatcttcccg
     2221 atgtttaccg taataaaaat tacggcgggt ccctattatc taaaatcagg agtctgttat
     2281 gcaaacgtgg ttaaatttgc aggataaaat cattattgtc accggcggcg catccggtat
     2341 tggtctggcg attgtagagg aattattagc acaaggcgcg aatgtacaga tggtcgatat
     2401 tcacggtggc gatggtcaat atgaaggtca taaaggctat cagttttggc cgaccgatat
     2461 ttctagcaca aaagaggtaa atcatacggt agcggaaatt atccagcgtt ttggtcgcat
     2521 cgacggtctg gtcaataacg ccggggtcaa tttcccgcgt ctgctggtcg atgagaaagc
     2581 gcctgccggg cagtatgaac tcaacgaagc tgcattcgaa aaaatggtca atatcaacca
     2641 gaaaggtgtt tttctgatgt cgcaggcggt ggcgcgacag atggtcaaac aacatgatgg
     2701 cgtgattgtg aatgtttcct cagaaagtgg gctggaaggc tcagaaggac aaagctgtta
     2761 cgccgctacc aaagccgcgc tcaatagctt cacgcgctcc tggtcgaaag agctgggtaa
     2821 acacggtatc cgtgtggtcg gtatcgcgcc cgggattctg gaaaaaaccg ggctacgcac
     2881 gccggaatat gaagaggcgc tggcgtggac gcgcaatatc accgtcgagc agctgcgtga
     2941 aggctatacc aaaaacgcca ttcctattgg gcgcgccgga agattagcag aagtagctga
     3001 ttttgtttgt tatctgttgt ctgaacgcgc cagctacatc accggagtaa ccactaacat
     3061 tgcgggcggc aaaacgcgcg gctaaggagg ctttatggtc aacgccattt tttgtgccca
     3121 cggcaaatta gcttgcgcca tgctggaatc ggttcagatg gtttacggca acgccaatgt
     3181 tgaagccgtt gcatttgtgc ccggtgaaaa cgccggagac attgcggtaa agctggaaaa
     3241 gttagtaagc attcacaatc aagatgagtg gctgattgcc gtggatttac agtgcggcag
     3301 cccgtggaat gcggcagcaa tgcttgcgat gcgcaatcct cgtttgcggg taatcagcgg
     3361 cctttctctg ccgctggcgc tcgaactggt tgataaccag gacagcatga atgttgatga
     3421 actgtgtgaa cacctgacgc aaatcgcaaa gcaaacctgc gttgtctgga aacaactggc
     3481 aacgacagag gaggatttct gatgaacatt acgctcgccc gcattgatga ccgtctgatc
     3541 cacggccagg tcactaccgt ctggtcgaaa gtggctaacg cccagcgcat tattatctgc
     3601 aatgacgaag tttataacga tgaagtgcgc cggacattat tgcgtcaggc tgcgccaccg
     3661 ggaatgaagg ttaacgtggt caatattgaa aaagccgtcg cggtttatca caacccgcaa
     3721 tatcaggacg aaaccgtttt ttatcttttc actcgcccac aggatgcctt agcgatggtt
     3781 cgccaggggg taaaaatagg caccctgaat attggcggca tggcctggcg gcccggtaaa
     3841 aaacagttaa ccaaagcggt ttcattagat gatgatgata ttaacgcatt tcatgagtta
     3901 aataatcttg gtgtcatact ggatttacgc gtcgtagcgt cagatccctc aatcaatatt
     3961 atcgacaaaa taaacgaaca gttaatcgca aattaaataa tagcgcctgt gtacatatgc
     4021 tttaacaggc agggatgacg acacttaaaa ggtgacaaat catggaaata agtaccctac
     4081 aaatcattgc tatatttctt ttttcctgta ttgccggaat gggcagcgtg ctggatgaat
     4141 ttcagactca tcgcccgtta attgcctgta cggtgattgg tttaattctc ggtgatttaa
     4201 aaaccggaat aatactcggc ggtacgctgg aattgatagc tctctgctgg atgaacgtcg
     4261 gcgcggcgca atctccggat tctgcactcg ccagcataat ctccgccatt ctggttatcg
     4321 ttggtcagca aagcatcgcc accggaatcg ccatcgcgtt gcctgtggct gcggcaggcc
     4381 aggtgctgac ggtgtttgcc cgtaccatca ccgtagtgtt ccagcacgcg gcggataaag
     4441 cagcagaaga ggcgcggttt cgcactctcg atattctgca tgtctccgcg cttggcgtgc
     4501 aggcgctgcg cgtcgccatt ccggcactga ttgtctcact gttcgtcagc gccgatatgg
     4561 tcagcaatat gttaaacgcc attcccgaat ttgtgacccg aggactacag attgctggcg
     4621 gttttatcgt ggtggtcggt tacgccatgg tgctacgtat gatgggcgtg aaatatttga
     4681 tgcctttctt tttcctcggt ttcctcgcag gtggctacct cgatctcagc ctgctggcgt
     4741 tcggtggcgt cggcgtgatc atggccctgg tctacatcca gctaaatcca cagtggtgta
     4801 aagctgaacc tcaactccag gccaccgcct ccaccgccct tgaccaactt gacgattaac
     4861 ggagccatca tggaacagag aaaaattaca cgcagcgatc tggtgagcat gtttctgcgc
     4921 tccaacctgc aacaggcgtc ctttaacttt gaacgtattc acgggctggg cttttgctac
     4981 gacatgatcc ccgccatcaa gcgactttac ccattaaaag aggatcaggt tgcggcgctc
     5041 aggctacacc tggtgttctt caataccacg ccagccgtat gtggcccggt catcggcgcg
     5101 gaaattgatg acggtaccat caacggcatc aaagtcggtc tgatgggacc attggcagga
     5161 gttggcgatc cactggtctg gggaacgctg cgcccgatta ccgccgcgct cggcgcatct
     5221 ctggcacttt cgggcaacat tctcggcccg ctgctgttct tctttatttt caacgcggtg
     5281 agtctggcga tgaagtggta tggcctacag ctcggctttc gcaaaggggt gaatatcgtc
     5341 agcgatatgg gcgggaatgt gctgcaaaaa ctcaccgaag gcgcgtcgat tctcggactg
     5401 tttgtgatgg gcgtgctggt gaccaaatgg acgtcaatca acgtaccgtt ggtggtttca
     5461 caaacgcatg ccgccgatgg ctccaccgtc accatgaccg tgcagaacat tctcgaccaa
     5521 ctttgccctg gtttgctggc gctcggtctg acgctactaa tggttcgtct gctcaacaaa
     5581 aaaattaacc cggtatggct gattttcgcc ctgtttggct tagggattat cggcaatgct
     5641 ctgggcttcc tgtcctgatt cttcgccccg gcacgactgc cgaggccatc gctcaacatg
     5701 aggtggttta tgaaaacaac agctctgcgt ctttatggta aacgtgattt acgcctggaa
     5761 acctttgacc ttcctgaaat gcaggaggat gaaatcctcg caacggtggt cactgacagc
     5821 ctgtgcctct cttcctggaa agaggccaat ctgggtgaaa accataaaaa agtacccgac
     5881 gatgtgacga ccaacccaat catcatcggc cacgaatttt gcggcgatat tctggccgtg
     5941 ggtaaaaagt ggcagcacaa attccagccg ggtcagcgtt atgtgattca ggccaacctg
     6001 caactccccg accgcccgga ttgccccggc tactccttcc cgtgggtaga cggcgaagcg
     6061 acgcatgtgg ttattcccaa cgaggtcatg gaacaatatt gcctgctggc atacgacggc
     6121 gaaacctatt ttgaaggctc actggttgaa ccgctttcct gcgtgattgg cgcgttcaac
     6181 gccaactatc atcttcagga aggtagttat aaccacacga tggggattcg cccgcaaggg
     6241 cacacgctga tcctcggcgg caccggacca atgggactgt tggcgattga ttatgcgctg
     6301 catggaccag ttaacccgtc gctgctcgtc attaccgata ccgacaacga caaattgagt
     6361 tatgcgcgca agcactatcc atcagaaccg caaacactga ttcattatct caatgccacc
     6421 gatgcagcat ttgatacgtt aatggcgctg agtggcggtc acggcttcga tgatattttc
     6481 gtctttgtgc ctaatgaagg actggtgact ctcgcctctt ccttgctggc gacagatggt
     6541 tgcctgaatt tcttcgccgg accgcaggat aaacatttca gcgcgccaat taatttctac
     6601 gatgtgcatt atgcatttac ccactacgtg ggcacgtcag gcggcaatac cgacgacatg
     6661 cgcgcggcgg tcaaactgat cgaagagaaa aaagtgcagg ccgcaaaagt ggtaacacat
     6721 attcttgggc tgaatgccgc gggcgaaacc acgcttgaat tgcctgccgt cggcggcggc
     6781 aaaaagctgg tgtataccgg gaaatacctg ccgctgacgt cactcacgca gattcaggat
     6841 caagcactgg cggcgattct ggcgcgtcat caggggatct ggtccggtga ggtggagcaa
     6901 tacctgctcg cccatgcaga ggcaatttcc catgattaat cgcgctactt aactctgcat
     6961 gtcgctggct ggtcgcccca gcaacttcgc gaccggatgt gcggctgatt gctgaagcag
     7021 cacaatttgc acgagaagga taaaaaatat cagcgcctta aggggcgctc aaattggtgg
     7081 caaagtacac attgtttatg ccggatgcgg aatgaagcct tattcggtct acataatttt
     7141 gaaaatccaa tatattgcag ttatctggta ggccaggtaa gcgtagcgca tcaggcaatg
     7201 ctgtgtttgt catcagtctc agcgcccggt gcaatacgtg acatcgtgag ccatgtcagt
     7261 aagtggttaa attcgcagtg gtagcatgcc ttccgtgatt tcttactaac cggagaagta
     7321 atggaactgc ttttattgag taactcgacg ctgccgggta aagcctggct ggaacatgca
     7381 ctgccgctaa ttgctgaaca gttgcagggt cgccgctcag cggtgtttat ccctttcgct
     7441 ggcgtaacgc agacctggga tgattacaca gagaaaacgg ctgcggttct cgctccgctg
     7501 ggtgtttctg tcaccggtat tcatagcgtt gtcgatcccg ttgccgcgat tgaaaatgcg
     7561 gaaattgtga ttgtcggcgg cggtaatact ttccagttgc tgaaacagtg ccgcgagcgc
     7621 gggctgctgg ctccaattac tgacgtggtt aaacgtggcg cgctgtatat tggctggagc
     7681 gcaggcgcta accttgcttg cccaaccatt cgtaccacca acgatatgcc gattgtcgat
     7741 ccgcaaggtt tcgatgcgct aaatctgttc ccgttgcaaa tcaacccgca cttcaccaac
     7801 gcgctgccgg aaggccataa aggtgaaacc cgtgagcagc gtattcgcga actgctggtc
     7861 gtcgcgccag aactgacgat tattggtctg ccggaaggta actggatcac agtgagtaaa
     7921 ggtcacgcta cgctgggtgg cccgaacacc acttatgtgt ttaaggctgg tgaagaagcg
     7981 gttccgctgg aagctggtca ccgtttttaa gtgaattgcc ggatgcatga tccggcatct
     8041 tgttcatagt gcctgatgcg acgcttgacg cgtcttatca ggcctacagg attcacaata
     8101 ttaatactcg tcccgtcctt catcttcatc cggctgttcc agcacactgt aagccaccga
     8161 acaaaacagc gagttcaggc gctgcatatc tcccagtaag cctaaatgta gcgaactggt
     8221 ttcaatgctc tgcacgtttt gctgatgcag gcgatcgacg tgggcgtggg aatagcggcg
     8281 attaagaatg cgaaaacgat gtttgctgcg acgcaaacga cgagcgctgg tgacatcgcc
     8341 agagaagaac accgacattg ccagctttaa attactgagc aattgctcat agagcgcatc
     8401 cagttccttc aacccatcaa gcgaaaatgc ccgccgtgct gccagcgatt tatcggcaat
     8461 ttcgctgccc atgcgctcga cgatatcgga ggcctgttca aggttgagcg acatttcgat
     8521 gatctccgcc cagcggcgcg actcttcctc cgccaactct tcttttggca tccgcgccag
     8581 atacagctta atggcggtgt agagaacgtt gatatcatct gccagcttac gcagctcttt
     8641 ctcctgccgt ggctcgccgt gcatcacttt attcagccct tccatcatct gttccatggc
     8701 gtcgccaatg cgcagggttt cgcgcgcggc gttcgccaga gcaagcgtgg gcgtgtccag
     8761 cgcgctgaca tccagatgtt taggccgtag ctgggtatcc agttccggtt catcgcgaat
     8821 aatcgttttg caaaaccgtg ccatcgggtc aacaaatggc agcatgacca ggcaacgtac
     8881 aaggttgtag aagacgtgga aatagatcac cagttccgct tttggcaatg acagcttccc
     8941 cattgtctct gccagcaaat ggacaaacgg caggataatc aggctaccca ccagcttaaa
     9001 cagcagacta cccagcgcga cacggcgggc tgcggcattg gcggcactgt tgttgagcat
     9061 cgccagcagg ccggaaccga ggttagctcc aatcaccaga cagagcgcca cggggaagga
     9121 gataatgcct gcagcggtca gagtcgcggt cagcagtaca gcagcaaggc tggagtagct
     9181 gataatggcg aacatcgcgc caatcagcgc atccagcaga atatcgccgg tcagcgaggc
     9241 aaagatcacc tgaacgccgt ttgcctgggt gatcggcgtt acggcctgca caatcaactc
     9301 cagcgccagc aaaatcagcc caagaccaat accgacgcgg cccagttgcc cggcgcgtga
     9361 ctgtttgcgt ccgaggaaaa aaatcacgcc gataaaaata agtaacggtg acagccagga
     9421 taagtcgaag gtgagaatac gcgccattag cgccgtcccg acatctgccc ccagcacaat
     9481 gaccagagcc ggtgcgaggg ctaccagatc ctgagcgacg aacgaggtca ccagcatggt
     9541 ggtggcatta ctgctctgta ccagtgcggt aacgccgatc cccgcgcaaa aggcgagcgg
     9601 ctttttttcg acgctccggc taaggacagt acgcaaacgc gcgccgaaga cgcgcattac
     9661 gccggttcga acaatatgag tcccccagac cagcagggcg acggcagaaa gcaggtgaag
     9721 cagcgttaac acgagttcag attctcctta tcgttatatg ttcctgaagc aattgaatga
     9781 ccaaaggacc ggaaacacgc tgactggcat ctttactgtg ccttttgaca ttcgtttttc
     9841 cggctaagat tattccagcc tgtttccagt ataagtgttt catattaata aagagacagg
     9901 gcactcattg agcgccctgt ttgttgtaag gaaaagtgac agatttgtga atcagtccgc
     9961 gtcatacccc agattcggtg ccagccagcg ctcaacatcg gagacgctca tacctttacg
    10021 gcgggcatgg tcttcaacct gatcgcgctg aatttgcgcc accgcgtagt acttgctgtc
    10081 cggatgactg aaataccaac ccgaaaccga tgcaccaggc cacatcgcga aagattctgt
    10141 gagtttcatg ccagtatgtt tttccacttc cagcagctcc cagatggtgg ctttttccgt
    10201 atgttccggg caggccggat agcccggtgc cggacggatg ccctggtagt tttcgcggat
    10261 cagttcttcg ttgctgaggt tctcgttcgg cgcatagccc cagtagactt tacgcacacg
    10321 ctcatggaga tactccgcaa aggcttcggc taaacggtcg gcaagcgctt tcaccatgat
    10381 tttgttgtaa tcatcgtgct gcgcttcaaa ggcatcagcc agtgcgtcct cttccagccc
    10441 gccagtcacg gcaaatgcgc cgatgtaatc tgctttacca gaaagcttcg gcgcaacgaa
    10501 gtcagcgaga cagtagttag cgaatcccgt tttttcggtc tgttgacgca gatggtggct
    10561 gacgttgatc acatgggtgc gcgtttcgtc acggtagatt tcaatgtcat cgcccacacg
    10621 gtttgccggg aacaggccca ccacgccacg cggattcagc gttttctcgg cgcttaattt
    10681 atccagcatg tcgttggcgt ctttaaacag ccgctgcgcc tcaacgccca ccacttcatc
    10741 ttccagaatg cgaggatact tcccggccag cgaccaggtc ataaagaacg gtgtccagtc
    10801 gatgtaatta cgcagcgttt cgatgctggc ttccacttcc tgcacgccga gacggtgcgc
    10861 caccggcggc gtgtaagcct gccagtcaaa tgcgaaatcg ttatcgcgcg ccgcttccag
    10921 cgtgaccggt ggtgtgcgtg gtttcttgcg cccgtgctga atacgcacgg tttcgtactc
    10981 cttgcaggta cgagcgacaa aatcatcacg ctgagtatcg gaaagcagcg ccgctaccac
    11041 accaacggtg cgcgaggcat tctgcacata caccgtaggg ccgctgtagt tctgctcgat
    11101 tttcaccgcc gtgtgcgctt ttgaggtggt cgcgccgcca atcagtaacg gaatggcgaa
    11161 gccctgacgc tccatctctt tcgccacgtt aaccatctcg tccagcgacg gcgtgataag
    11221 ccccgaaagg ccaatcaaat cagcattcac ttctttggcg atacggagaa ttttttccgc
    11281 aggcaccata acgccgagat cgacaatttc gtagttgtta cattgcagca ccacaccaac
    11341 gatattttta ccgatgtcgt ggacatcgcc tttcacggtg gcgatcacca tcttgccgtt
    11401 ggttttaccc tgctctttgc tggcttcaat aaacggttcg agatacgcca ctgcctgttt
    11461 catgacgcgc gccgatttga ccacctgtgg caggaacatt ttgccttcgc caaacagatc
    11521 gccgaccacg ttcatgccgt ccatcaacgg gccttcaatc acttcaatcg ggcgcgtagc
    11581 ctgctggcgg gcttcttcag tatcctgctc gataaactcg gttatgcctt tgaccagcga
    11641 gtattccaga cgtttattca cgtcccacga gcgccactcc gcttgctggg cgttagcagt
    11701 gtcgtccgct ttactgccgc gatatttctc ggcaagctcc agtaaacgct cagtgccatc
    11761 gtcacgacga ttaagaatca catcttccac cgcgtcgcgc agttcagcgg gtaggtcgtc
    11821 gtaaatcgcc agttgcccgg cgttgacgat ccccatatcc atgccattgc gaatagcgta
    11881 gtagaggaac actgcgtgaa tggcttcgcg caccggatcg ttgccacgga atgagaaaga
    11941 aacgttagat acgccgccgg aaatcagcgc gtgcggcagt tcgcgtttga tgtcttcaca
    12001 cgcgccgata aagtcctgcg cgtagttgtt gtgctcttca ataccggtgg cgaccgcgaa
    12061 gatgttcggg tcgaagatga tatcttctgg cgggaagcca acctcttcgg tgaggatttt
    12121 gtacgcccga cggcaaatct cgattttccg tgcgcgagta tcggcctgtc cctgttcgtc
    12181 aaaggccatc accaccactg ccgcaccgta gcgacgcaac aatttcgcgt gatggataaa
    12241 ggcgtcgacg ccctctttca tcgagataga gttaacaatg cctttgccct ggatacactt
    12301 cagacctttt tcaatgacat cccattttga ggagtcgatc ataatcggca cgcgagcgat
    12361 atccggttca ccggcaatca gattgagaaa acgcaccatc gccgcttcgg catcgagcat
    12421 cccttcatcc atgttgatat cgataatctg cgcgccgttt tccacctgct gacgcgccac
    12481 atccagcgcc tcgctgtatt tctcttcttt gatcaggcgc ttgaacttag cagagccggt
    12541 gacgttggtg cgttcaccca cgttcacaaa caggctatcg tcgccaatgt tcagcggctc
    12601 caggccagac aaacggcagg ctacgggaat ttccggcagt ttgcgcggcg ctaatccttc
    12661 tactgcacga ctcatcgctg caatatgttg cggcgtggtc ccgcagcaac caccgacgat
    12721 attgagaaag cccgcttgcg cccattcacg tatctgtttt gccatcgtgt cggcgtcgag
    12781 atcgtattca ccaaaggcgt taggcagccc ggcgttcggg tgggcggtga cgtagcattc
    12841 cgcaatccgt gatagttcct gcacgtactg gcgcagttca tcgggcccca gcgcacagtt
    12901 caggccaaag gtcagcgctt cggcgtggcg caatgagttg taaaatgctt cggtggtctg
    12961 cccggagagc gtgcgcccgg aggcgtcggt gatggtgccg gagatcataa tcggcagctc
    13021 aacgcccagc gcttcaaact ccgttttcac cgcaaagacc gccgctttgg cgttaagggt
    13081 gtcgaaaacg gtttcaatca ggatcagatc cgcaccacct tccaccagcg ctttggtgga
    13141 ttcgcgatag gccgccgcca gctggtcaaa agtgatgttg cggaatgccg gatcgttgac
    13201 gtccggagaa atagacgccg tgcggttggt tgggccaaga acaccggcaa cgtagcgcgg
    13261 tttctccggc gtgcgcgcgg tccactcgtc agcacaggcg cgcgccagtt tcgccgccgc
    13321 aaagttgatt tccgccgaca gggattccat ctggtaatcc gccatcgcaa tggtcgtgga
    13381 gttgaaggtg ttggtttcga tgatatccgc gcccgcttca aagtaggcgt tgtggatagc
    13441 ggcgatcact tccggtttac tgagtaccag caggtcgttg ttgcctttga ggtcgcatgg
    13501 ccagtcggca aagcgttcac cacgaaaatc ggcttcgttc agtcgatagc tctggatcat
    13561 ggtgcccata ccgccgtcca acaccagaat acgttcattt aactgcgcac gcagttgttc
    13621 cactttgctg ctcacacttg ctcccgacac gcttaaccca gatttaataa aaattcaaca
    13681 aaccatactg gaataaacgc atctgtggta aaagcgacca ccacgcaaca tgagatttgt
    13741 tcaacattaa ctcatcggat cagttcagca actattgcat tagctaacaa taaaaatgaa
    13801 aataatttcc acgatacaga aaaaggaggc tgtcatggtc gcacccattc ccgcgaaacg
    13861 cggcagaaaa cccgccgttg ccaccgcacc agcgactgga caggttcagt ctttaacgcg
    13921 tggcctgaaa ttactggagt ggattgccga atccaatggc agcgtggcac tcacagagct
    13981 ggcgcaacaa gccgggttac ccaattccac gacccaccgc ctgctaacca cgatgcaaca
    14041 gcagggtttc gtgcgtcagg tcggcgaact gggacactgg gcaatcggcg cacatgcctt
    14101 tatggtcggc agcagctttc tccagagccg taatttgtta gcaattgttc accctatcct
    14161 gcgcaatcta atggaagagt ctggcgaaac ggtcaatatg gcggtgcttg atcaaagcga
    14221 tcacgaagcg attattatcg accaggtaca gtgtacccac ctgatgcgaa tgtccgcgcc
    14281 tatcggcggt aaattgccga tgcacgcttc cggtgcgggt aaagcctttt tagcccaact
    14341 gagcgaagaa caggtgacga agctgctgca ccgcaaaggg ttacatgcct atacccacgc
    14401 aactctggtg tctcctgtgc atttaaaaga agatctcgcc caaacgcgca aacggggtta
    14461 ttcatttgac gatgaggaac atgcgctggg gctacgttgc cttgcagcgt gtattttcga
    14521 tgagcaccgc gaaccgtttg ccgcaatttc tatttccgga ccgatttcac gtattaccga
    14581 tgatcgcgtg accgagtttg gcgcgatggt gattaaagcg gcgaaggaag tgacgctggc
    14641 gtacggtgga atgcgctgac tttttctggc gggcagaggc aatattctgc ccatcatacc
    14701 tgagtggcaa tagaataagg gtgtctgtta atcgcattga cgccaaaata acttaatgtc
    14761 atacacttca cacactttta ttaactcagc attattttta aacatcaaac cacttaatta
    14821 ttaacaaaat cgcaagatct cattccaccg aaacttttac ggctgcatta tgagattgag
    14881 cgatttatga tattttttta gttgtaatca tattaaattt tttcactgtt ctgatagtta
    14941 aaattcaaga catcaataaa caatgagata tttaaatgat tactcgtatt cctcgtagtt
    15001 ctttctctgc aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat
    15061 tgttttataa agaactcgag gataaatttt ctggcaagga gctggcgacg cctctattaa
    15121 aaagcttctc agagaactgt agacaaaatg ttcgtcatat ctttagcaac aaggattttg
    15181 tcattaaatt ttccacgtcc gtcttacaag ctgataagaa agaaattacg atgattaata
    15241 aaaacgaaaa cacgacactc actcaaacca ttgctacaat atttgaagaa tacctaatgg
    15301 aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccaa aaatcagata
    15361 gaaaagaaaa agaattccca agaattaaac ttaatggtca atgttatttc ccggggcgcc
    15421 ccaaaaccgt atagtatacc gacacattgc tgcacaatat attaatgata tttatcagaa
    15481 tgttgattac aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt
    15541 caacaaaaaa tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg
    15601 cgttgctgcc tgcggtgatt tcgggctagt tatgaaagca tattttgaca agatggaatc
    15661 aaatggcatc agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg
    15721 gctaagaata aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc
    15781 taatgtaact aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta
    15841 ttctctgatg gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatgt
    15901 tattaaccaa tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa
    15961 aggaactgtg gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc
    16021 aattggtcag aatcaccaac ttacgcaact gatgattcag ctccagaaaa tgccagaact
    16081 acatagaaca gaaatgttga ctgcctataa tagtattaac ctccccggtt tatatttggc
    16141 tataaattat ggcaatgcgg atatcgttga gactattttc aattcattgt cagaaacagg
    16201 atatgaaggg ttactctcga aaaaaaatct catgcatatt ctggaggcaa aagataaaaa
    16261 tggtttttct ggattatttt tagcgatatc acgtaaggat aaaaatgttg taacctcgat
    16321 tctgaacgtc ttacctaaac tggccgcaac acatcattta gataatgaac aggtgtataa
    16381 attcctgagt gccaaaaata gaacgtccag ccatgtttta taccatgtta tggcgaatgg
    16441 tgatgccgac atgctgaaaa tttttttggt cgcgttacct ttgttaattc gcacatgtca
    16501 tttgactaaa gaacaggtac tcgatctcct gaaggcaaag gatttttatg gttgcccaag
    16561 actatacctg gcgatgcaaa atggacatag cgatatcgtg aaagttattc tcgaagcatt
    16621 gccctgtctg gcccaggaaa ttaacatttc agcttccgat attgtagatc ttctgaccgc
    16681 taaaagtctt gcgcgcgaca cgggtttgtt tatggccatg cagcgcggac atatgaacgt
    16741 tattaatact atttttaacg cattacccac tctttttaat acgtttaaat tcgataaaaa
    16801 aatatgaagc ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg
    16861 atacagcata aacaacaaaa cgttgtagag acggtttatc ttgctttatc tgaccatgca
    16921 cgcctgtttg gatttaccgc tgaagatatt atggattttt ggcaacacaa agcgccacaa
    16981 aaatactctg cctttgagtt ggcttttgaa tttggtcacc gggttattgc tgaattaatc
    17041 cttaatacat taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt
    17101 gcggagaaaa attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa
    17161 atctttgcct gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt
    17221 gtagtgcgcg ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac
    17281 caatacgcgg gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg
    17341 agacgctgta ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt
    17401 cgcggaaatt cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc
    17461 cgtgacgggt gacaccgaag tttttaaaca gcatgtcgcc agggaaaatg ttagcagcgg
    17521 caagctggcg aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca
    17581 cttgttccag ccagatattg agcggcacca tccgccgctc gattatagag atggcgaatt
    17641 acaatctgct caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt
    17701 aataccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc
    17761 attcggccca cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca
    17821 gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc
    17881 ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca
    17941 ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg
    18001 cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac
    18061 tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa
    18121 acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc
    18181 gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc
    18241 agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg
    18301 aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt
    18361 ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc
    18421 cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc
    18481 tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac
    18541 cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac
    18601 agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta
    18661 gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg
    18721 tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg
    18781 gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca
    18841 atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga
    18901 tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc
    18961 gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg
    19021 gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcagt ggagccagtc
    19081 agcgcggtga ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct
    19141 gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc
    19201 ggctgctgca ctttctcaac atagtgcttc ataccttcgc cctgcgcata agcgtttgcc
    19261 aggtcgaaca tgttaaacca catgctgtgg atacctgcta gggtgatgaa ctggaacttg
    19321 tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc
    19381 tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg
    19441 atagcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc
    19501 aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga
    19561 gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc atacgggtcg
    19621 caatctgagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag cagggttgga
    19681 acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt
    19741 ggcactaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg
    19801 aagtgaactg ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg
    19861 acaccgccaa aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc
    19921 ggatcgcccg gctcaatgcc cacggaccat tggatctgat cggcacgacg gaaggtgttg
    19981 ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac
    20041 atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca
    20101 atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gttgttgatg
    20161 tagccttttt tcgactcacc gtgcagcaga cgccacattt tcgctgcgcc cagttgcgcc
    20221 agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc actgtatggg
    20281 cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc aatttgttgt
    20341 gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct aacaggcggt
    20401 agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt tccatcaagc
    20461 gtgcggcatc gtcaaaacgg ccctgggaga aacgttcttc gcccagttcg ctggcaatga
    20521 ctttcatctc ttcgcccagc atctggcgga acaaggcttt ggtcaccggt ttgccattgc
    20581 tcaacgtttt ttgatgatgg atccactgcc agatcgaggt acgggaaatt tcagccgtcg
    20641 ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc cacgcttcga
    20701 tgtactgcac agccacgcga atgttagcgc gcataccttc ttcggtgcgt tcaccatcac
    20761 aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcaat acttcaagct
    20821 gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtgtcc gcaaggcctg
    20881 ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta tccgctttta
    20941 ctttgttgag cacctggtta ttacgctctt catctttgct cggaataaac gcggccatgc
    21001 cgcccatcgc aaaagcaccg cgtttatggc aggttttaat cagcaggcgt gagtaagcat
    21061 tcaggaatgg tttatccatc gtcacggcct gtctgtctgg caggacacga tcgggatagt
    21121 ttttcaacgt tttgatatag ctgaagatgt aatcccagcg accgcagttc agaccaacaa
    21181 tatggtcgcg cagcgcgtga aggatttcgt ccatctggaa cacggcgggc agcgtttcaa
    21241 tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct gcatagctga
    21301 agacttcgct ccaccaggcc gcttcctgct aggactgggt tttcggcaga tagaaatagg
    21361 gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc gcaaaatcaa
    21421 acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc aagtgcagac
    21481 cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt ttgcctgctt
    21541 cattggtata gctgatggta ccgttaaccg catcgcgcag gttaatttgc ccgtcgatca
    21601 ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact ttcacattgg
    21661 cgttcagtgc gttgatcacc atcttgcgct ctaccgggcc agttatctct acgcggcggt
    21721 cttctaagtc cgcaggaatc ccgcgaattt tccaatcagc atcgcgaatg gaagccgttt
    21781 ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga atgcgcgctg
    21841 ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga aattctaccg
    21901 cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttgtgaaa gccagttcat
    21961 cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg aaaacttccc
    22021 ctctgtgaac gaaggaacgt tgtgcgcttt tcgttcagca caactaagac tactcattta
    22081 aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac ttattgataa
    22141 caatatgaat aaaatttatt cacttgctga ggtgcgtttc ggaaataaaa aaggcacccg
    22201 aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga agattaatcc
    22261 agcgttggat tcatgtgccg tagatcgtat ggcgtgatct gatagacgta atagttgagc
    22321 cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggttt gttttgcgga
    22381 tcgttgtgcg ggaaatagtt atacggtaca tccgggtcta gtccggcttc cacatcgcgt
    22441 aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac aaaggcaatg
    22501 cgcttatctt tgctggcaaa cagatatgca tccccttctt ccgtctctgc cagaatttcc
    22561 agatcggtgt aatcacgaat caacgctgcc ggaaagtcag catagcgcga atgcggtgcc
    22621 aggaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat atgatgctcg
    22681 taaacgccag agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag gatattgagc
    22741 gcggcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt cgaccactcc
    22801 agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag gcccagcggc
    22861 gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa gttacagtag
    22921 aagttgttca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat gcgcaacagc
    22981 tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt ttcagtttca
    23041 atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc ctgaccagac
    23101 gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac ggcgggtagc
    23161 tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt atacgtttag
    23221 acatccagat agctgaagat aaccagaaaa tttgccaatg tcgagcctgc atgttgaagg
    23281 tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa ggagagaaaa
    23341 tggttattag tattcgccgc tcacggcatg aggaagggga ggaactcgtt gcgatttggt
    23401 gtcgatctgt cgatgccacc cacgattttt tatcagcaga gtatcgggcc gagctggaag
    23461 agctggtgcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat gaacgggagc
    23521 agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt atcgatcctg
    23581 atgtgtgcgg ctgcggcgtg ggtcggatgc tggtggagca tgcgctctcg atggcaccgg
    23641 aactgacaac caacgttaat gagcaaaatg agcaggcggt tgggttctat aagaaggtgg
    23701 gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat ccgttgctga
    23761 atctggcgta tgtgggggaa taagccgcct ctgccgtgcc tgcccaagct ggagaagacg
//
LOCUS       DAJTYH010000062        23452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTYH010000062 DAJTYH010000000
VERSION     DAJTYH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..361)
                     /locus_tag="OO609_002424"
     CDS             complement(<1..361)
                     /locus_tag="OO609_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6633807.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSA"
     gene            579..1439
                     /gene="tesB"
                     /locus_tag="OO609_002425"
     CDS             579..1439
                     /gene="tesB"
                     /locus_tag="OO609_002425"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6633808.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1488..2774)
                     /gene="amtB"
                     /locus_tag="OO609_002426"
     CDS             complement(1488..2774)
                     /gene="amtB"
                     /locus_tag="OO609_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6633809.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2804..3142)
                     /gene="glnK"
                     /locus_tag="OO609_002427"
     CDS             complement(2804..3142)
                     /gene="glnK"
                     /locus_tag="OO609_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6633810.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3323..5104)
                     /locus_tag="OO609_002428"
     CDS             complement(3323..5104)
                     /locus_tag="OO609_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6633811.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5097..6869)
                     /locus_tag="OO609_002429"
     CDS             complement(5097..6869)
                     /locus_tag="OO609_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6633812.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(6899..7357)
                     /gene="decR"
                     /locus_tag="OO609_002430"
     CDS             complement(6899..7357)
                     /gene="decR"
                     /locus_tag="OO609_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6633813.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7511..8329)
                     /gene="cof"
                     /locus_tag="OO609_002431"
     CDS             complement(7511..8329)
                     /gene="cof"
                     /locus_tag="OO609_002431"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6633814.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8429..10121
                     /locus_tag="OO609_002432"
                     /pseudo
     CDS             8429..10121
                     /locus_tag="OO609_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10186..10881
                     /gene="queC"
                     /locus_tag="OO609_002433"
     CDS             10186..10881
                     /gene="queC"
                     /locus_tag="OO609_002433"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6633815.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(10933..11331)
                     /gene="fadM"
                     /locus_tag="OO609_002434"
     CDS             complement(10933..11331)
                     /gene="fadM"
                     /locus_tag="OO609_002434"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6633816.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11425..11796)
                     /locus_tag="OO609_002435"
     CDS             complement(11425..11796)
                     /locus_tag="OO609_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6633817.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(11947..13818)
                     /gene="ppiD"
                     /locus_tag="OO609_002436"
     CDS             complement(11947..13818)
                     /gene="ppiD"
                     /locus_tag="OO609_002436"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6633818.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14010..14282)
                     /gene="hupB"
                     /locus_tag="OO609_002437"
     CDS             complement(14010..14282)
                     /gene="hupB"
                     /locus_tag="OO609_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6633819.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14491..16845)
                     /gene="lon"
                     /locus_tag="OO609_002438"
     CDS             complement(14491..16845)
                     /gene="lon"
                     /locus_tag="OO609_002438"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6633820.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17033..18307)
                     /gene="clpX"
                     /locus_tag="OO609_002439"
     CDS             complement(17033..18307)
                     /gene="clpX"
                     /locus_tag="OO609_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6633821.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18433..19056)
                     /gene="clpP"
                     /locus_tag="OO609_002440"
     CDS             complement(18433..19056)
                     /gene="clpP"
                     /locus_tag="OO609_002440"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6633822.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19303..20601)
                     /gene="tig"
                     /locus_tag="OO609_002441"
     CDS             complement(19303..20601)
                     /gene="tig"
                     /locus_tag="OO609_002441"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6633823.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(20945..21262)
                     /gene="bolA"
                     /locus_tag="OO609_002442"
     CDS             complement(20945..21262)
                     /gene="bolA"
                     /locus_tag="OO609_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6633824.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21567..22145
                     /locus_tag="OO609_002443"
     CDS             21567..22145
                     /locus_tag="OO609_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6633825.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22189..>23452
                     /gene="ampG"
                     /locus_tag="OO609_002444"
     CDS             22189..>23452
                     /gene="ampG"
                     /locus_tag="OO609_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6633826.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEY"
BASE COUNT         5516 a         6501 c         5648 g         5787 t
ORIGIN      
        1 ccgcactcaa cagaatacgc gcgttcggct gaacatctgc cgggttaaat ggcagaacaa
       61 agctgaatgg tgactgttta ccttcagtac gcaccgcttt ctgcgccagc acttttgacg
      121 gtgcatcggc taacgacgcg tcagaaagtg tcacggtcag cacagcatca ggcggcagtg
      181 cgactttctg acggatccag acagtaccgg agacattcgg ttgctggata gcaggttgtt
      241 gtgttgctga aatagacgta tttgcagccg gggctggcgt ctgaatatcc gcgcttttat
      301 ctgcgcaagc cgccaacgca atcgcaaccg ctaaaccact ggccatgtgc acgagtttca
      361 ttcatttctc cttattatca atgcaccagc gggctaactt tcctcgccgg aagagtggtt
      421 aacaaaatag taacgtcaac aagtgtggca cacatcacgc atttctgcct gtaattagcc
      481 cgtaattcag actatttcac ccatcggacc agtggcaaat ttgcgttata ctcaactcac
      541 tttggcttgc tgcggcagct ttgttactgg agagttatat gagtcaggcg ctaaaaaatt
      601 tactgacatt gttaaatctg gaaaaaattg aggaaggact ctttcgcggc cagagtgaag
      661 atttaggttt acgccaggtg tttggcggcc aggtcgtggg tcaggccttg tatgctgcaa
      721 aagagaccgt cccggaagag cggctggtac attcgtttca cagctacttt cttcgcccag
      781 gcgatagtaa gaaaccgatt atttatgatg tcgaaacgct gcgtgacggt aacagcttca
      841 gcgcccgccg ggttgctgct attcaaaacg gcaaaccgat tttttatatg acggcctctt
      901 tccaggcacc agaagcgggt ttcgaacatc aaaaaacaat gccgtccgcg ccagcgcctg
      961 atggcctgcc ttcggaaacg caaatcgccc aatcgctggc gcacctgctg ccgccagtgc
     1021 ttaaagataa attcatctgc gatcgtccgc tggaagtccg tccggtggag tttcataacc
     1081 cactgaaagg tcacgtcgca gaaccacatc gtcaggtgtg gatccgcgca aatggtagcg
     1141 tgccggatga cctgcgcgtt catcagtatc tgctcggtta cgcttctgat cttaacttcc
     1201 tgccggtagc tctacagccg cactgcatcg gttttctcga accggggatt cagattgcca
     1261 ccattgacca ttccatgtgg ttccatcgcc cgtttaattt gaatgaatgg ctgctgtata
     1321 gcgtggagag cacctcggcg tccagcgcac gcggctttgt gcgcggtgag ttttataccc
     1381 aagacggcgt gctggttgcc tcgaccgttc aggaaggggt aatgcgtaat cacaattaaa
     1441 aaaatagccg gaggtgaaaa ccgtccggct attttttgca gtgcttgtta cgcgttatag
     1501 gcattctcgc cgtggctgtt gacgtccagc ccttctcgct cttgttcttc cggcacacgt
     1561 aggccaaccg tcagatccgc cagtttgtag ccaataaatg ccaccacacc ggaccagacg
     1621 atcgtaatgg cgatgctttc caactgtacc agcaactgat ggcccatcgt cacaccttca
     1681 gcgaagccca cgccgcccag agaactggcg gcaaaaatcc cggtcatgat acagccgaca
     1741 atgccacaaa cgccgtgcac accgaagaca tcgcagggat catccacccg cagcaagcgt
     1801 ttgagcatgg taacgcccca caaacccgcc agaccagcta ccacgccgat aatcaacgcg
     1861 ccgccaaccc caatgtagcc gcacgctggc gtcacgccga ccagaccggc aatcgcgcca
     1921 gaacacgctc ccagcagaga aggcttacca cgcagcgccc attcaccgaa gatccagcca
     1981 agaattgccg ccgccgttgc gaccacagta ttcacaaatg ccagtgccgc gatttcattc
     2041 gccgtgccca ctgacccggc gttaaagcca aaccaaccga tatagagaat ggcagtcccg
     2101 gtgaagacca tcggcaggtt gtgcggttta aacgcctctt taccgaagcc tacgcgtttg
     2161 cctatcagat acgcgcccac cagaccggcg attgctgcgt taatgtgcac caccgtgcca
     2221 cccgcgaaat ccagcgcacc gtgagaagcc agcaaaccac cgccccacac catatgcgca
     2281 atcggaatgt aagagagcgt cagccatacc accacgaaaa tcaacacagc tgagaagcgg
     2341 attcgttccg ccagcgcccc aactatcaag cctacggtaa tgcaggcaaa cgatccctga
     2401 aacgctacgt ggatatactg ataaatgctg cccatcaccg ccgtcagttc gatgtttttt
     2461 agcatcaacc agttaatgtt gccgaagaag ttgttgccct caccaaacgc cagcgaataa
     2521 ccgtaaacca cccagagaat acagaccagt gcaaacgtca ccgtcacctg cgtcagcatc
     2581 gacagcacgt ttttgccgcg aatcaaccca ccgtaaaaca gggcaatccc cggaatagtc
     2641 ataaacagca ccagcgcagt acaaatcatc ataaacgcat tgtcggcttt atcggccacc
     2701 gcaggtgcag ccattaccag tcccggcagc atcgccagtg aagcaagccc agtttttatc
     2761 gtcgctatct tcatttttcg ttcctgttgc tgtgtgccag agattacagc gccgcttcgt
     2821 cggcttcgcc ggtacgaata cgaatgacgc gttgcaattc agcgacgaag attttgccgt
     2881 cgccaatttt tccggtgtaa gccgccttac tgacgatatc gatcacttca tcgagttggt
     2941 catcagcaat cgccacatca atttttactt ttggcaggaa gttgacgctg tattccgccc
     3001 cccggtacag ctcggcatgc cctttctgac gcccgaaacc tttcacttcg gtgacggtca
     3061 ggccctgaat accaatggaa gataacgctt cacgaacgtc ttccagcttg aatggtttga
     3121 ttatcacggt caccagcttc atagatcccc tccggtcaga attcggtaat ggttctgcta
     3181 cacgaagaag gtattgcaag cggtgtgcca gaaatgaaaa acgagaaaga gagcaggaag
     3241 ttaagaagat tgaaaatgaa aacgcactat gacagtgcac agtgcgttac atttttgcac
     3301 caacgatgaa atattgcgct attcaggcgc tcaatgattc ctcttcacgc acgctggctg
     3361 ccagctcttc gcccgcaagt tgcagttgat acatctgcca gtagcgtccc tgggccgcca
     3421 gcagttgctg gtgagtgccc tgctccacgg cttgcccacg atgaagcacc agaatggtgt
     3481 cggcatcaat aatagttgat aagcggtgag caatcaccac cagcgtggta tgttcacgca
     3541 ccgccgccag agcatgttga atcgcctgtt cagtaccgga gtcaatgctg gcggttgcct
     3601 catcaaggat caggatttgc ggcgtctcca ccagcacgcg cgccagtgcc agcagttgct
     3661 tttgcccgac tgagagatta tttccctgct cgcccagcgg cgtataaata caatcactca
     3721 tgctgcgcgc cagctccgcc agttgcacgg tttccagcgc ctgccagacg cgttcttcgg
     3781 agatatcccg ccccagcgtc acattagcga ggaaggtatc cgccagcacc accggatctt
     3841 gctgcaccat tgccacgccc tggcgcagcg cgctatgact tagcgaactt aatggacgac
     3901 catcaaggtg aatctcacct tccgttagcg ggtaatagcc catcaataaa ctggcgaggg
     3961 tacttttacc actgccggta tgcccgacca gcgccacaaa gttacgcgaa ggcacagaga
     4021 gattaatgtt ctttagcacc agattgtcat cgcgataagc aaatgacacg ttatcgactt
     4081 cgatggtgcc actctgtaac gggtaatcat cattgccata ttgctggcgc ggtccgtcca
     4141 tcagttcaaa cacgcgctca ccagcaacaa cagcctgttg cagcatcgcc tgttgcgtgg
     4201 tcagttcgat taagggttcg ttaagtcgcc caagatagct gataaacgca tacaacacgc
     4261 ccacttcaat ggtgccgctg gcggagaagc caaacagcat caataagcca caaagaatga
     4321 gcgatgaaaa cagactcagc agcggacgca gcagaaaacc gtcgaggcgc agggtttgca
     4381 tcctagccat atagtgtgaa cgactggcct cccccatacg ttcgccaaat cgcgcctgct
     4441 gacgaaactg ctggataacg ctcatgccat tgatgatttc gttaaagccg tcgttgatat
     4501 ccgccagata ggcgcgcaca cggcggacaa tcggcgtgct gtaacgctgg tatatcacca
     4561 ttaccaccag caccaccggg aaaatcatta tcgccaccag cgccattcgc cagtcgaggc
     4621 tgaacatcgc caccagcatc gcgcccacca gtgcggcact gcgcaggaca gttgccacta
     4681 cggtaacgta gagatcgcgg atcacttcag tgtcattagt gacgcgggaa atcacctgcc
     4741 cgacgggttg ggtatcaaac tcgcttaatg gctggcgtaa cgcagcatcc atcacgtcgg
     4801 tacgcaactg ttgcactgcg cctactgccg cccgattaaa cagcagcgac tgcgcgtaat
     4861 gtagcccggc ggcaaacagt tgtagcccaa catacgccgc agccagccct gcaaccactt
     4921 tcaacggcag gttatttttc gctaccatat tgtcgataaa atagctgata agcagcggcc
     4981 cactgacttc tgccgccgcc gcaacccaca tcatcaggac cgcaatcccc agcggtttac
     5041 gccacggcga accatacgct aacaggcgct tgagagtcgg ccacagttgg ctaaaactac
     5101 gcatcgacgg cctcctcgcg aatttccgga acgtcgtcga gcgccgcctc cagttgttga
     5161 tagcgataca tatcgcgata ccaaccgctt tgttgcgcca gctcatcatg attgccacgc
     5221 tgggcgatat gtccgtgctg catcacaata atttcactgg cttccgtcag tgccgaaagg
     5281 cgatgggcac tgatgattac cgttcttccc tgtccccact gacgcaggtt atgcaggatc
     5341 tggtgctctg tgcgtccgtc caccgccgaa agcgcatcat caaggatgag gatttccgcg
     5401 ttgactaata acgctcgagc aatggagata cgctgttttt gcccaccgga aagcatcacg
     5461 ccgcgctcgc ccacttctgt atcgtaacct tgcggtagac gcaaaatatc gtcatgtacg
     5521 ctggctaacc gcgcgacatg ctcaatctct tgctgggtgg cattcgggca acccagcgcg
     5581 atgttattcg ccacggtgtc agaaaaaagg aatggcgtct ggctgaccac cgccaggcgg
     5641 ctacgccagc tatcgagttg taacttcgtc agaggaatat catgaaagcg aatatccccc
     5701 tcgctgacgt cgaaatgacg ctgaatgagc gacaacaggg tacttttgcc ggaaccagtc
     5761 ggcccgcaaa tacccagcat ctgaccgggt ttcagggcga aattgacgtt ttccagcgca
     5821 ggatggtcag tctgcggata cgtgaactgg tgaatattta catccagttc gccacgccct
     5881 tccggcacgg gttcgctacc atcgttcacc accggcgctt ccgccagcat ggcgcgaata
     5941 cggctgtatg cagcactacc acgttccaca atgttaaaca tccatgccag cgccagcatt
     6001 ggccaaatca tcagacctaa atacatcata aaactggtga gctggcccag cgttaaactg
     6061 ccctgcacca ccatccaact accgccgcca atcgccagca agttcgccat accaatcgcg
     6121 atatagatgg tcgggtcgaa gcgagcatca atacgcgcca cccgcatgtt ttttttgccg
     6181 gtatcttcgg catccgcggc aaataacgcc gactggcgat cttccagacc aaaggctttg
     6241 atcatgcgga tactggtgag gctttcctgg gtgcggtcat taagactgga aaacgccgcc
     6301 tgtgccagct taaagcgttc atgcaaggca tcgccgttgc gcttaatcat gatcgccatc
     6361 actggcatcg gtaacagggc aaataaggtc aactgccagc taatttgcgt agacatcata
     6421 atcaacacag cgcagcccat taccagtgaa tccaccagcg tcagcacccc ttctccggcg
     6481 gcaaacacga cgcgatcgac gtcattggtc gcacgagcca tgagatcacc cgtgcgatga
     6541 cgcaggtaaa actccgggtg ctgccggctt agctgacggt aataatcttc acgcagttca
     6601 acagccagtt ggtaagacgc gccaaacagc aatacgcgcc agacataacg caggagataa
     6661 accacaacgg caatcagcac catggtggcg atccacatca ggctcagccc ggtagtaaag
     6721 tgttgttctg tcacgccatc gacaacaata ccaaccactt ttggcggaac cagttgcagc
     6781 atcgcgataa tgacaagcaa ggcgacagcc ccaagatagc gacgccattc ccgacggaaa
     6841 taccagctta attgagcaaa taatcgcacg cgttgtgtcc tgacctgatt ctggatattt
     6901 attcgatggg taaagaagtg gtgtatttaa tctgttccat cgcgaagctg gaagtgacgt
     6961 ccgacagccc cggcacgctg tttaccagac gcttataaaa ctcgtcgtag cgtttcatgt
     7021 cggcaacctg gacgcgcatc agataatcgt attcaccagc catgcgccag acccccagca
     7081 cttctggcat ttcggtaacc accgtgacaa agcggcaata ccattcgctg ctgtgatgtt
     7141 gcgtttttat cagcacaaag gcggtcaggc cgaggcctat tttttccgga tccagcaggg
     7201 cgactttgcc gataaggata ccgtcgtcct ccagccgttt caggcgcttc cagcaggggg
     7261 tggttgtcag attaacggct tcagccagtg cctgcaaaga gagggtgcaa tcctgctgca
     7321 gtaaggccag cagcttacgg tcaattttat ctaacatagc ccttccacgg agaatttttt
     7381 tctcttttaa ttctatttta aaggtgaaat cgcgacaatt tattctgtgc ggtttaaaca
     7441 ccacggcaca aaatgacaaa ttcaacgcaa aaaaaaaccg ggcaattgcc cggtgctgga
     7501 agggatctcg ttattcgggg gaataaggta gatgtggata gtccagccag tgcgtcaaat
     7561 agtgagagac agcctgattt cggcaatgtc caatcaccgg taaatgcggg agctccgcgc
     7621 gcagttgcgg catcgcattg cccataataa acccgctacc gacgctgcct aacatttcgc
     7681 gatcgttcat cgcatcacca aaggccatgc aatcgcgcaa cgataaacct aaatgttggg
     7741 tccgcaccgt caatgcagcg cctttattgc agcccaccgg cagcacttcg aggcaatccg
     7801 tggcggaaaa acacaaatgt gcacgctcgc ctaatgcttc gtatagctgg atctgcaagc
     7861 gtgtaagatc gtcgtgatcg ccacagaagc agatcttggt gacgctgccg agtggcattt
     7921 ttttgacatc gattatctga taacgaaaac cgctatagac aaatacctgc aacaacgcag
     7981 ggctctcttt cccggtaaac caaccgtcgt cattgaagat atgcatgctg gctcgggtat
     8041 cccattgctg atacagcacc agctccgcga catccgcagg taaatcatca cgatgtaaaa
     8101 gttcaccttc cagagaatgc acgcgcgttc cgttgccggt aatcaaatat gcatccagcg
     8161 atagcgcccc gagaatatgc tgcatctcca gcgcatgacg ccccgtggca aaagtgaggg
     8221 taatgtcgcg ttcacgcagt cgcgccaaag tagagagggt tttctcacct aaatgatggt
     8281 caggcatcaa taaagtgcca tccatatcaa atgctaccag acgagccatt tctttctcca
     8341 ctctgtgaca cggttaatgg tgattgagta tcacctgata tatacggaag taatagtgaa
     8401 tagttaaata atattgttcc gggtttatat gcgattgctc aaccgtctta accagtatca
     8461 acgtctgtgg caaccttcag ccgcaaaccg tcaccgtcag cgaactggcc gaacgctgtt
     8521 tttgcagcga acgccatgtt cgtacgctgt tgcgtcaggc acaggaggcg ggatggctgg
     8581 agtggcaggc gcagtcagga cgcggaaagc gcggacaatt acgctttctg gtcacgccgg
     8641 aatcgctacg caatgcgatg atggaacagg cactggaaac cggaaagcag caagatgtgc
     8701 tggagctggc gcaactggcc ccaggtgagc tgcgcactct gttacagcca tttatgggcg
     8761 gacaatggca aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     8821 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     8881 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     8941 aaacctctac tgacaggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9001 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9061 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9121 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9181 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9241 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9301 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9361 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccaag
     9421 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9481 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9541 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9601 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9661 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9721 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
     9781 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
     9841 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
     9901 tgcaatcgac gctggacgcg gtgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
     9961 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10021 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10081 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10141 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10201 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10261 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10321 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10381 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10441 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10501 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10561 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10621 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctgatgtg gattgataaa
    10681 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10741 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    10801 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    10861 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    10921 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    10981 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11041 ctgcccttcc ggctccagtg taatgacctg gtttaagatg ccgcttttac cgtttaattg
    11101 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctggacgac gatagttaat
    11161 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11221 attttccacc ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11281 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11341 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11401 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11461 cgaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11521 ttcgcgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11581 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11641 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11701 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    11761 cagtgtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    11821 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    11881 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    11941 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12001 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12061 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12121 attaccttgc atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12181 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12241 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12301 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12361 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12421 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12481 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12541 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12601 ctctgcaccg gccagagact cggtgtcgtt gcttgccgca tcgctcactt tctgctgcag
    12661 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12721 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    12781 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    12841 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    12901 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    12961 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13021 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13081 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13141 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13201 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13261 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13321 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13381 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13441 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13501 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13561 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13621 catacgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13681 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13741 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    13801 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    13861 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    13921 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    13981 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14041 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14101 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14161 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14221 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14281 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14341 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14401 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14461 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14521 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14581 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14641 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14701 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    14761 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    14821 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    14881 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    14941 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15001 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccga ttacctgacc
    15061 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15121 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15181 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15241 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15301 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15361 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15421 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15481 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15541 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcggattttt
    15601 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15661 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15721 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    15781 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    15841 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    15901 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    15961 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16021 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16081 cttcagggct tcgttttcgt ccggcacgtc gtccatttca ccgagttctt tctgaatagc
    16141 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16201 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16261 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16321 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16381 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16441 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16501 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16561 taacccctcg accagcactt tgacggtgcc gtcaggcagt ttcagcatct gcaatataga
    16621 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16681 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16741 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    16801 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    16861 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    16921 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    16981 ggggagataa aatcccccct ttttggttaa ctaattgtat gggaatggtt aattattcac
    17041 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17101 ttaccgactc gtcgataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17161 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17221 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17281 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17341 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17401 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17461 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17521 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17581 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17641 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17701 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    17761 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    17821 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    17881 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    17941 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18001 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18061 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18121 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18181 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18241 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18301 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18361 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18421 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18481 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18541 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgggc
    18601 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18661 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18721 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    18781 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    18841 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    18901 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    18961 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19021 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19081 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19141 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19201 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19261 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19321 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19381 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19441 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19501 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19561 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19621 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19681 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19741 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    19801 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    19861 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    19921 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    19981 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20041 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20101 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20161 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20221 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20281 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20341 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20401 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20461 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20521 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20581 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20641 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20701 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    20761 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    20821 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    20881 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    20941 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21001 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21061 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21121 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21181 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21241 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21301 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21361 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21421 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21481 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21541 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21601 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21661 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21721 agcgtaccga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    21781 cctcccgcgc tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    21841 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    21901 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    21961 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22021 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22081 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22141 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22201 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22261 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22321 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22381 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22441 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22501 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22561 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22621 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22681 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22741 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    22801 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    22861 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    22921 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    22981 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23041 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23101 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23161 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23221 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23281 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23341 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23401 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tc
//
LOCUS       DAJTYH010000063        22930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTYH010000063 DAJTYH010000000
VERSION     DAJTYH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            81..857
                     /gene="exuR"
                     /locus_tag="OO609_002445"
     CDS             81..857
                     /gene="exuR"
                     /locus_tag="OO609_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6633827.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1202..1864
                     /locus_tag="OO609_002446"
     CDS             1202..1864
                     /locus_tag="OO609_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6633828.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1868..2251
                     /gene="mzrA"
                     /locus_tag="OO609_002447"
     CDS             1868..2251
                     /gene="mzrA"
                     /locus_tag="OO609_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6633829.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2398..2752
                     /locus_tag="OO609_002448"
                     /pseudo
     CDS             2398..2752
                     /locus_tag="OO609_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2790..3095
                     /locus_tag="OO609_002449"
     CDS             2790..3095
                     /locus_tag="OO609_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6633830.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3098..3502
                     /locus_tag="OO609_002450"
     CDS             3098..3502
                     /locus_tag="OO609_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6633831.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3492..3791
                     /locus_tag="OO609_002451"
     CDS             3492..3791
                     /locus_tag="OO609_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6633832.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3977..4273
                     /locus_tag="OO609_002452"
     CDS             3977..4273
                     /locus_tag="OO609_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6633833.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4343..5329
                     /gene="yqjG"
                     /locus_tag="OO609_002453"
     CDS             4343..5329
                     /gene="yqjG"
                     /locus_tag="OO609_002453"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6633834.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5622..5990
                     /locus_tag="OO609_002454"
     CDS             5622..5990
                     /locus_tag="OO609_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6633835.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6197..7093)
                     /gene="yhaJ"
                     /locus_tag="OO609_002455"
     CDS             complement(6197..7093)
                     /gene="yhaJ"
                     /locus_tag="OO609_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6633836.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7198..7899
                     /locus_tag="OO609_002456"
     CDS             7198..7899
                     /locus_tag="OO609_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6633837.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7922..8086
                     /locus_tag="OO609_002457"
     CDS             7922..8086
                     /locus_tag="OO609_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633838.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8298..9608)
                     /locus_tag="OO609_002458"
     CDS             complement(8298..9608)
                     /locus_tag="OO609_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6633839.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9636..10967)
                     /locus_tag="OO609_002459"
     CDS             complement(9636..10967)
                     /locus_tag="OO609_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6633840.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11242..12606)
                     /gene="tdcG"
                     /locus_tag="OO609_002460"
     CDS             complement(11242..12606)
                     /gene="tdcG"
                     /locus_tag="OO609_002460"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6633841.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12678..13067)
                     /locus_tag="OO609_002461"
     CDS             complement(12678..13067)
                     /locus_tag="OO609_002461"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6633842.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13081..15375)
                     /gene="tdcE"
                     /locus_tag="OO609_002462"
     CDS             complement(13081..15375)
                     /gene="tdcE"
                     /locus_tag="OO609_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6633843.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15409..16617)
                     /gene="tdcD"
                     /locus_tag="OO609_002463"
     CDS             complement(15409..16617)
                     /gene="tdcD"
                     /locus_tag="OO609_002463"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6633844.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16643..17974)
                     /gene="tdcC"
                     /locus_tag="OO609_002464"
     CDS             complement(16643..17974)
                     /gene="tdcC"
                     /locus_tag="OO609_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6633845.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17996..18985)
                     /gene="tdcB"
                     /locus_tag="OO609_002465"
     CDS             complement(17996..18985)
                     /gene="tdcB"
                     /locus_tag="OO609_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6633846.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19084..20022)
                     /gene="tdcA"
                     /locus_tag="OO609_002466"
     CDS             complement(19084..20022)
                     /gene="tdcA"
                     /locus_tag="OO609_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6633847.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20212..20556
                     /gene="tdcR"
                     /locus_tag="OO609_002467"
     CDS             20212..20556
                     /gene="tdcR"
                     /locus_tag="OO609_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6633848.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20812..21352
                     /locus_tag="OO609_002468"
                     /pseudo
     CDS             20812..21352
                     /locus_tag="OO609_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21374..21721
                     /locus_tag="OO609_002469"
     CDS             21374..21721
                     /locus_tag="OO609_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633849.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21890..22540
                     /locus_tag="OO609_002470"
     CDS             21890..22540
                     /locus_tag="OO609_002470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633850.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6185 a         5780 c         5286 g         5679 t
ORIGIN      
        1 aaatttatat atgaccaatg gcatacccat gaataaagtg gtatgataac ttcgtccgca
       61 ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat caacaacttg
      121 ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat aaactgcctg
      181 ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt gaagccatca
      241 tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt catgtggttt
      301 ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc aattacggtc
      361 cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag ttcgcggcaa
      421 ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag gcgcgcggcg
      481 aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta gccctggcga
      541 cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt agccataacc
      601 cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac tggtgtgatg
      661 accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct aagctggcaa
      721 tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc gacgatttcg
      781 agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc gatacggcca
      841 ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg tttgtctcgt
      901 cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc ctcccctgca
      961 atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt tgacctttgt
     1021 tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca cgttttaaca
     1081 gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa acagcaataa
     1141 cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc aggaataatg
     1201 aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg aaaccctggc
     1261 caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt tccttgaaaa
     1321 tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg tcggcgtgtt
     1381 gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg ttgccgccag
     1441 cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc gcaccgtaca
     1501 aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc tttttcataa
     1561 acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa cactgctgcc
     1621 gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca actggatgag
     1681 cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca aaacgccggt
     1741 atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg tggtgctgct
     1801 ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg gaaatcgggg
     1861 gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg gcactgtttt
     1921 acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag agtctacgct
     1981 ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga tctggcatca
     2041 ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca ctctgttaat
     2101 tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca gaacattgcc
     2161 ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt ggctgacccg
     2221 gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca tatcactcac
     2281 tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca ctaccgcttc
     2341 gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg ttcaagaatg
     2401 aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag ttatgccact
     2461 accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc cgaaaaacac
     2521 caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg ggccaactgt
     2581 tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa agatgaggtg
     2641 gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga tagaacggaa
     2701 actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact aactcacaat
     2761 agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg gaacatctgc
     2821 gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca tctggcgaga
     2881 agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg aaacagagcc
     2941 gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc gcggcggcgc
     3001 gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc gctgcaatcg
     3061 gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc atcacgcgca
     3121 agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca tggttgaaat
     3181 ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag ctaatctctt
     3241 tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc ttatgagcct
     3301 gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga tgattgccac
     3361 caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac gtaaatcgcg
     3421 taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc aactgctcga
     3481 ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa ctgcttagcc
     3541 agatccagca acaacggctg gatatttccg ccagtagtcg tgaatggctg gaggcaacag
     3601 gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg ctggttggca
     3661 gtagagtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc tgggccagac
     3721 gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag cagcaacagc
     3781 ttcgcggtta acttccctct ggccggagca attccggcct tatccatcaa attttttgaa
     3841 tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga ttctctccat
     3901 cgacagcaac gacgctaata gcgcgccctt gcacaaaaaa acaatcagca gcctgagtgg
     3961 cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag cgcacatttt
     4021 aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact ctggtaccca
     4081 acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ctgacggcat ttttatttca
     4141 cagcaacttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga caatttctgg
     4201 cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcggcc gcctgctgaa
     4261 taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa gctcctcgtt
     4321 tttgctattg gaggagagaa aaatgggtca actgattgac ggcgtctggc atgacacctg
     4381 gtacgatacc aaatctaccg gcggtaaatt tcaacgttca gcttccgcat ttcgtaactg
     4441 gctcactgcc gatggcgcac ctggccccac tggcacaggc ggttttatcg cagagaaaga
     4501 tcgttatcat ctctatgttt cactcgcctg cccgtgggcg caccgcacgc tgatcatgcg
     4561 caaactcaaa ggactggaac cgtttatttc cgtttccgta gtgaacccgc tgatgctgga
     4621 aaacggctgg acctttgatg acagttttcc gggagcaacc ggagatacgc tctatcaaca
     4681 tgaatttctg tatcagcttt atctccacgc cgatccacac tacagcggac gagttactgt
     4741 tcccgtgctg tgggacaaaa agaaccacac catcgtcagc aacgaatcag cagaaatcat
     4801 ccgcatgttc aataccgcgt ttgatgcgct gggcgcaaaa gcgggtgatt actacccacc
     4861 agccctgcaa acgaaaattg acgaacttaa cggctggatt tatgacaccg ttaacaacgg
     4921 cgtgtataaa gctggttttg ccaccagcca gcaagcctac gacgaggcgg tggcgaaagt
     4981 gtttgaatcg ctggcgcgac tggagcagat tttaggccag catcgttacc tgaccggcaa
     5041 ccagctaacc gaagccgata ttcgcctgtg gactacgctg gtgcgttttg atccagtgta
     5101 tgtgacccac ttcaagtgtg ataagcaccg catcagcgat tacctgaatc tgtatggctt
     5161 cctgcgcgat atctaccaga tgccggggat cgccgaaact gtcaatttcg atcacatccg
     5221 taatcattac ttccgcagcc ataagaccat caaccctacg gggattattt caattggtcc
     5281 gtggcaggat ctcgatgaac cgcatggacg agatgttcgc ttcggttaaa aaaaggcacc
     5341 ttacgaggtg cctctgataa ttagctaagt gcatgttgtt catgtcggat gcggcgtgaa
     5401 cgccttatca aacctacaaa gcgtttatat tcaatatgtt acaatgaccg cgtaggccag
     5461 ataagcgtag cgcatctggc gattttgcgc ttgccatcag tcttagcgcg ccttcacggc
     5521 actttattaa ttccttgaac gaatatttac tgccatttca ttcacgtcta ttcttaattt
     5581 gctgctttaa aaacaagtga ttgagcaaaa ttgaggcaaa aatggactgg tatctgaaag
     5641 tactaaaaaa ttatgtcggt ttccggggtc gtgcgcggcg caaagagtac tggatgttta
     5701 ttctggtcaa catcatcttt acgttcgtgc tggggctgct ggataaaatg ttaggctggc
     5761 aacgcgctgg tggcgaaggc atcctgacga taatctacgg tattctggtg tttttaccgt
     5821 ggtgggcggt tcagttccgc cgcctgcacg acaccgaccg ctcggcgtgg tgggcactgc
     5881 tattcttaat cccgtttatc ggctggctta ttatcatcgt ctttaactgc caggcgggta
     5941 cgccaggcga aaaccgcttt ggaccagatc caaaactgga acaagaataa tgattaagca
     6001 ccttgtaaaa ggtgcttcaa attgttgaca aagtgcgctt tgtttatgcc ggatgcggcg
     6061 taaacgcctt atccagccta caaattgcca aaattcaata tattgcagtg actgcgtagg
     6121 cctgataagc gtagcgcatc aggcagtttt gcgtttgtca gcagtcagaa gcaccttgtg
     6181 aaaggtgctt aatagattat tttccagcga acaactttgg aatttcccgc agacaccagg
     6241 atttcgcttc ccccatactg tcacgacgcc aggccataat aatatcgatc tcgctggtcg
     6301 attccgggct gacgacacgc aaccgccctt ccgcaatatc tttttcgacc atcggatacg
     6361 gcatcgtcgc cacgccaagc cccgccagta atgcctgacg tttatcttca atcgtgctca
     6421 ccgttaagcg cggctgtttg tccagcagct gtacggtcaa caccgggcgc tcacgagcgg
     6481 tatccgctac cgcaattcca cgatatttca cacgcgtcac ttcagataac ggttccggct
     6541 cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt cattaacgta tagagcttgc
     6601 gcgagttgat ctccgacgag gaacgaaaat gcatatccgg cgcgataacg atatccgccc
     6661 gcccctgctc cagccgttcc cacgcccccg ccagcacttc tgtgatgatt gccagttggg
     6721 tattggcttt tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg
     6781 cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc gagagcttcc gcatcagtag
     6841 tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc ctcccgacat
     6901 tggtgaattt ggtgcgatgg cccgagcggt caaacagcac cacatccagc tcttcttcca
     6961 gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc cagctcatcc gctgccgccg
     7021 caaaactgcc ccggcgatcg atcgcatcca taacccgtag tgcttccagc gttaatgccc
     7081 tttctttggc catttcgttc tcattcagga aatttgaaca taccgggcag aatatctggc
     7141 taacaatgta gcgtccagcc ccttaccata aaaggaagta aagagaggtc aagaattatg
     7201 attactaccc gaactgccag gcagtgtgga caagcagact acggatggtt gcaggcccgg
     7261 tatacttttt cctttggaca ctacttcgac ccgaaattgt taggctatgc ctccctgcgt
     7321 gtgcttaacc aggaagtgct ggccccaggt gccgcctttc agccgcgaac ctatcccaaa
     7381 gtcgatattt taaatgtgat tctggatgga gaagcagagt atcgcgacag cgaaggcaat
     7441 catgttcagg ccagcgccgg agaggcgttg ctgctctcta cccagccggg tgtcagctat
     7501 agcgaacaca atctcagcaa agacaaaccg ttaacgcgaa tgcagctttg gctggatacc
     7561 tgcccgcagc gagagaatcc gctgattcaa aagctggcgc ttaatatgga caagcagcaa
     7621 ttaatcgcct cgccagaggg tgcgatggga agcctgcaat tacgccaaca agtgtggctg
     7681 caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg
     7741 tatttgcaat cgattcacgg gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg
     7801 ctaacctgcg gtgatggggc gtttattcgt gacgaggcta acattacgct ggttgccgat
     7861 tccccactgc gcgctttgct gatagatttg cctgtctagt tgtttttaca gggagatgat
     7921 gatgagtaaa aaatcgacca aaaagcgcca gccagcgaag cccgtggtgg cgaaagaacc
     7981 tgctcgcacc gccaaaaatt ttggctatga agagatgttg agcgagctgg aagctatcgt
     8041 cgcggatgct gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac aggttgccgg
     8101 atgcggcgta aacgccttat ccggcctaca aaacacaaca acatcaacaa attaaacacc
     8161 ccgtaggccg gataagatgc gccagcatcg catccggcat caggtacaaa acacaacaac
     8221 atcaacaagt tgagcacccc gtaggccgga taaggtgcgc cagcatcgca tccggcacaa
     8281 tactcaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat ctgccgatcc
     8341 gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg ctcaacatca
     8401 tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat taacaccgct
     8461 ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg
     8521 caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat agtttcataa
     8581 cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt cgttgcggca
     8641 caaagtgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa atgagaaagc
     8701 atcagcgcac gcgccagtcg ttcatcatcc gctccgaagt gttctgctac aaccaccaca
     8761 ggcattattg cagtgatccc ctggttaccc gagccggagt tactcatagc cggaagcgta
     8821 gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga
     8881 tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc
     8941 cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga atcgagaata
     9001 aagcggatcg ccgcaaatgg gacttcattg acgaacttca ggatctcagc cagcgtcgtt
     9061 ctggaaagca ccgtcagcgg cgactcttgc tcgccctctg ccacacacgc ctggtgggta
     9121 aacaccacac cattgtgcgt ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg
     9181 acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat ttcattgcaa
     9241 ggttcctgga tcttaacaga gactttcccc gccgccagca gtgctttggc atcggtaatt
     9301 gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc acctaacgcc
     9361 cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt gacgcccaga
     9421 ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg accttccagt
     9481 tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc
     9541 ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt taaagtcgaa
     9601 tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag gaacagagaa
     9661 acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt gtatttgtgc
     9721 aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat gccgaaaatc
     9781 gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc gctccaggcc
     9841 agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat cttctcggca
     9901 ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa
     9961 tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct gaccactttc
    10021 acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc
    10081 tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag
    10141 aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa tgctttatgc
    10201 cgcgccactt caattgattt ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc
    10261 tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc
    10321 agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac catcgacacg
    10381 cccagcgcag cgactaccag cagcttggtc agcaccatgt cggtcgaaat tttgaataac
    10441 aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag accataaaag
    10501 ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga agcactatcg
    10561 ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa atagagcgca
    10621 cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg gtaatcttta
    10681 cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata cattgccggg
    10741 taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac ctgcaccggg
    10801 agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca gccggtgtca
    10861 gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc acgacccgcc
    10921 ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat ttctcgctca
    10981 atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg cggtctacgt
    11041 ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag aaaatcttca
    11101 caatcaagac tttaaaataa cggagatgga taagaatttt ctactaaatt aatcacaaga
    11161 gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt gtgcacccaa
    11221 ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc ctccgcgtga
    11281 tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct cgatcacttt
    11341 atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc
    11401 tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg
    11461 atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc atacctgcgc
    11521 cggactgccg cccagtagtt cagttaaccc tgccgctgcc attgaacagg ccacgccaat
    11581 ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat acagcgcgcc
    11641 aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg gacgacggaa
    11701 cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag tcggtgccgt
    11761 taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca tgttgatcca
    11821 gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct gacgacgcag
    11881 cgctacggca cgacacggca cattgagcgg accaggcagc acgccttcag tgttcatccc
    11941 gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt caatttccgc
    12001 tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca ggccgttgta
    12061 atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg acgtttcgac
    12121 atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga cagagtaata
    12181 ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc cgttctcatg
    12241 gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat caacaatatg
    12301 cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa atgcagggat
    12361 ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa tggcgacatc
    12421 cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac agatcgacca caatatggct
    12481 cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc cggcattcat
    12541 tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat cgaatgcact
    12601 aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga ccttgcgacc
    12661 gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc cagcttcaca
    12721 tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca
    12781 tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc ggtgatgaac
    12841 acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat cgctttgacg
    12901 ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc ggtctgcggg
    12961 caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc ctgaacataa
    13021 gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca tcctccggcg
    13081 tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc
    13141 gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt tttccgggtg
    13201 ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt gttgaccgcc
    13261 ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt tggttttacg
    13321 tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg agatcccatc
    13381 tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg cacctttgcg
    13441 gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc
    13501 cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca gaatcgactg
    13561 ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa agcgttcaac
    13621 caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc
    13681 gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca cgcgggcata
    13741 tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc atgccatagt
    13801 gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg
    13861 catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt ccatgaagtg
    13921 atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt
    13981 cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt agagcagcgt
    14041 tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca ccattgggct
    14101 gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt
    14161 ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt tgaaggcaat
    14221 cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg
    14281 cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt ccagccccat
    14341 cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat caaattccgg
    14401 tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca gttcctgtgc
    14461 ctgctgctca ttgaggacgc cagctttaaa gtcgcgctca atgtagatat ccaggaacga
    14521 tgccgtgcgc cccagcgaca tcgcgccgcc attttgcgat ttcactgccg ccagataagc
    14581 gaagtagagc cactgcaccg cttcctgtgc attctgcgcc gggcgagaga tatcaaagcc
    14641 atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg ccagctcctc
    14701 acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact ggagatcggc
    14761 aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta cgcggcgata
    14821 gtcaccgata atgcgcccac gaccatagcc atctggtaaa ccggtcagca cgccagattt
    14881 acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat gggttttacg
    14941 cagatcggta aacagatatt caaattcact gtccatttca cggccatagg cgtggaatga
    15001 acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt
    15061 ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat gagcggtaat
    15121 tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat
    15181 gccttccatt accttttccc acaattccgt ggtggcaggc gtcgcttcgg cgagaaaaga
    15241 ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat taatttcgct
    15301 tttccagtcc gtacctttaa agccaagcca tgcgtcagcg tacagcttat cgctggtatc
    15361 aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt atgcaaattc
    15421 tgcgggcgcg ttaactttac ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt
    15481 agtcggaata acgacgcaaa tgacatgcgc attttcactg gaaacaattc gctcgccaaa
    15541 agagttagaa cgattattca tttctgtatc aatctctacg cctaatacag ccaaatgttc
    15601 catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga atataattcc
    15661 atccagccga cgtaatgaag ctgcatgtcc ggcaatatga cgggcaattc ggtgaacaaa
    15721 ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt ccagaacacg
    15781 taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca ctacgcgttc
    15841 caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac caaagtcgac
    15901 atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca ttgaggtgtc
    15961 aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat gcgccacaac
    16021 caggccggag tcatcttccg ccagattcag cagcgaatgt gcgcgctggg aaacatagcg
    16081 gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat atttccacgg
    16141 caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat caaataccgc
    16201 cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta aatttgcgta
    16261 attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt catcggtaat
    16321 aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa ttaaggccac
    16381 gctgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg caccttcgta
    16441 gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg cattttccga
    16501 gttaataccg tcggcaatac ctgacattaa tacttcacaa tcgctggcat cgagtacgga
    16561 aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt cattcatctc
    16621 ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg atggtcagca
    16681 gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc agagacggcg
    16741 ctttacggat agcatacatc ggcaacaggc acagcaggga tgcgataatc ggtgcgccca
    16801 tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag gtggagccca
    16861 tgatgaagat catgctgaga gtattcagtt tacccagcga cactttggtt ttgtcacctt
    16921 tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga tagtgaccga
    16981 agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc agtgtaatcg
    17041 cgaacgttgt tttcgtaccg gtcatggacg caaagtggtt agccagataa gaaagcactg
    17101 gaatattctg cgctttggct tccgccatgt tggccggaga cagagtaaac aggcagctaa
    17161 aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg atttgggaac
    17221 atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt ttagaaacca
    17281 cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa atccccagcc
    17341 agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt
    17401 cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc aggctggcga
    17461 taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca aaccagatga
    17521 cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc
    17581 cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca taaatccaca
    17641 gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca gttttaccaa
    17701 aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa agacacagac
    17761 gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac atcagaagaa
    17821 tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg gcgccgattg
    17881 ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac gattgttttg
    17941 tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga attaattaag
    18001 cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg ccggaaataa
    18061 tactgacggt ttttctattt tgaatatatt ggtctaattt accgcttaat aatgcagcac
    18121 atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag gcaatcatac
    18181 tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca cgaacgattt
    18241 cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg ccggtagttc
    18301 ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg ttttctgact
    18361 gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca ataccagcaa
    18421 ttaaaccacc accaccaatt ggcacaatca cgttatcgac atcatagaga tcttccataa
    18481 tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa ggtgggataa
    18541 aaatacgacc ttccatttcg acaatttcgc tcactttagc gatagtgtcg ttgaagttat
    18601 caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg gattttggcg
    18661 caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc
    18721 cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc gcatcggtca
    18781 gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc tgcatatttt
    18841 caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag cgaggcatgc
    18901 ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca tcaatagcaa
    18961 ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa atatgtaggt
    19021 aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa taaaaacaaa
    19081 caactaacca acttctatta attgccttcg tctacaccca ttataagatg aatactcttt
    19141 ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg accataccgc
    19201 ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt gattagaacc
    19261 aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga gaacaagatt
    19321 ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc catttctttg
    19381 taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca cccactgttc
    19441 gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac tggctaccag
    19501 cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca tttctgcact
    19561 taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga acgaagacag
    19621 ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact tgttgatcat
    19681 ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt ccaccaccgc
    19741 ttcagaagac ataccgctta tctcattaac catatttttc atttcacggg taatggattc
    19801 ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat ttttccgcac
    19861 cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga cggccggttg
    19921 agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga cttcctgaaa
    19981 gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct acgttaatta
    20041 cctcattgac ggcatgaagt gtatcaaaat gaaataaaca ggatatgtgc gaccactcac
    20101 aaattaactt tcattacttt ccaaggtatc gttattacaa ttaaacatct ttaaatatca
    20161 acaagttaaa gcataaaaat cagcataaac cctgattttt atcaaaaaaa aatgacgggg
    20221 ataaccatat tttatggcga taacatcatt cgttatgtgg ttaatacaaa aaaggggctg
    20281 agaccatatt tcaagcaatt accggataat taccaggcaa aatttgagtt aaatttaatg
    20341 agcaagttct caaattttat tataaataaa ccattttcag cgataaatac tgcggcacgt
    20401 cacatttttt cacgctattt gttggagaac aaacatttat tttatcaata ttttaaaatt
    20461 tcgaatacag gtattgatca tctcgaacaa ttgattaacg tcaacttttt ctcttctgac
    20521 aggacgtcat tttgtgaatg caatcgtttt ccataaattc ttctcccctc ataggcgacg
    20581 aatagcattt tgtgttgagg atcacaaaac gaataattgc tggtcgccgc gataaggtca
    20641 gacgaagtca acaaggggga ttttcactgt gctttacatc agcgtaggac acagaatgtt
    20701 gcttcgttta ctgtaacgcc gggtaaatga gcgttttttg atagtgcgta agtgtcgtgc
    20761 gaggaaaata agcatctatt attgtttgtc atgacgacaa ggcaggcatc aatgaaaggc
    20821 ttcccaattg cacatatttt tcacccttca atcccgccaa tgcacgcagt ggttaacaat
    20881 cacaatagaa atattgatta ttggacggta aaaagaaagt ttgcagaaat tgtctccacc
    20941 aatgacgtta ataaaattta cagtataagt aatgaactcc ggagagtatt atctgcaatt
    21001 actgcattga atttctatca aggcgatgtt ccttctgtca tgattccgaa tccaaccgga
    21061 aaatatgagt ccattcatca tagatatttc tacaggagaa catgatgatt atatcataca
    21121 aacattagat gtaggcactt ttgcaccttt tggtgaacaa tgtacttgct cagccgtcaa
    21181 taaaaaagag ctggaatgta ttaaggagac gatttctaag tattgcgcaa agttcaccca
    21241 aaaagaagcc atcttaacac cccctaggca ctttaataaa acctgcatta cttcagattg
    21301 ttggcaaatt ctcttttttt cacccgatca ttttaataat gatttttatt gattatttat
    21361 aaaggataaa ataatgttcc ctgtttcatc aattggtaat gatattagta gtgatttagt
    21421 ccgtcgaaaa atgaatgacc ttcctgaaag ccccatagga aataacctcg aagcactagc
    21481 accaggtata gaaaaactaa aacagacctc tattcaaatg gtcactttac ttaacgcgtt
    21541 acaaccaagt ggaaaatgca ttatcactgg tgattttcaa aaagaattag cgtacttaca
    21601 aaatgtaatt ctttataatg actcgtctct tcgtctggat tttttaggtt ataacaccca
    21661 aattattcaa cgatcggaca atacctgcga acttaccatt aatgaaccgt taaaaaacta
    21721 ggaaatatct acaggcaata tcaatgttaa ttgcccatta aaagatattt acaatgaaat
    21781 caggaggttg aacgtaatct ttagttgtgg gactggagat atagttgatc tatcctctct
    21841 ggacttacgt aatgtcgatt tagattatta tgatttcaca gataaacata tggctaatac
    21901 tattctaaat cattttaaat tggattcaac aaattttact aatgccaaca tgtttcaggt
    21961 taattttgtt agttcaacac aaaacgccac aatctcctgg gattatttac taaaaataac
    22021 gcctgtttta ataagcatta gcgatatgta ttctgaagaa aaaatcaagt ttgtcgaaag
    22081 ttgtttaaat gagcttggag acattaccga agaacaatta aaaattatga gatttgcaat
    22141 tataaaatct ataccaaggg caactcttac agataaatta gaaaatgaat taacaaaaga
    22201 aatatataaa agctcatcga aaatcaacaa ttgcttgaac agaattaaat taccagagat
    22261 gaaagaattc tcatcaaaaa aaacatatga ttacatcgat acaatcattg aagattatga
    22321 aaatatcaaa gaaaatgctt atctggtcgt cccccaaatt aattatacta tggatttaaa
    22381 catagaagac tctagctcag aagagttaat accctcgaga aagacgaaaa ttctccggac
    22441 aatggctttg aggtcgggga atataacaca tatgaagcat ataactcaga gaatcaatat
    22501 tttaccagag aggactatac gtataattac gaccttttaa atgcaatata gacttaataa
    22561 cttcttacaa agtctattat taataccgta gatattcatc ttaccctgtg caccaaaagc
    22621 aaaaacttat cgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct gatgcgctac
    22681 gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt gtaggcagga
    22741 taaggcgttc acgcagcatc cggcatgaat aaagcgcact caacaatctg aaacccgccg
    22801 gaacggttta ttacgtacat caggtgaaac tgaccgataa gccgctttct tttgggtata
    22861 gtgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg ccgaagcggg
    22921 tttttacgta
//
LOCUS       DAJTYH010000064        22159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTYH010000064 DAJTYH010000000
VERSION     DAJTYH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..906)
                     /locus_tag="OO609_002471"
     CDS             complement(<1..906)
                     /locus_tag="OO609_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633851.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALV"
     gene            complement(1008..1988)
                     /locus_tag="OO609_002472"
     CDS             complement(1008..1988)
                     /locus_tag="OO609_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6633852.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1996..2706)
                     /locus_tag="OO609_002473"
     CDS             complement(1996..2706)
                     /locus_tag="OO609_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6633853.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2923..4746)
                     /gene="typA"
                     /locus_tag="OO609_002474"
     CDS             complement(2923..4746)
                     /gene="typA"
                     /locus_tag="OO609_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6633854.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5119..6528
                     /gene="glnA"
                     /locus_tag="OO609_002475"
     CDS             5119..6528
                     /gene="glnA"
                     /locus_tag="OO609_002475"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6633855.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6701..7750
                     /gene="glnL"
                     /locus_tag="OO609_002476"
     CDS             6701..7750
                     /gene="glnL"
                     /locus_tag="OO609_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6633856.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7762..9171
                     /gene="glnG"
                     /locus_tag="OO609_002477"
     CDS             7762..9171
                     /gene="glnG"
                     /locus_tag="OO609_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6633857.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9283..9393
                     /locus_tag="OO609_002478"
     CDS             9283..9393
                     /locus_tag="OO609_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6633858.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9622..10995)
                     /gene="hemN"
                     /locus_tag="OO609_002479"
     CDS             complement(9622..10995)
                     /gene="hemN"
                     /locus_tag="OO609_002479"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6633859.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11184..11693)
                     /gene="yihI"
                     /locus_tag="OO609_002480"
     CDS             complement(11184..11693)
                     /gene="yihI"
                     /locus_tag="OO609_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6633860.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12275..12907
                     /locus_tag="OO609_002481"
     CDS             12275..12907
                     /locus_tag="OO609_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6633861.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13289..16075)
                     /gene="polA"
                     /locus_tag="OO609_002482"
     CDS             complement(13289..16075)
                     /gene="polA"
                     /locus_tag="OO609_002482"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6633862.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16439..17371
                     /locus_tag="OO609_002483"
     CDS             16439..17371
                     /locus_tag="OO609_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6633863.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17412..18842)
                     /locus_tag="OO609_002484"
     CDS             complement(17412..18842)
                     /locus_tag="OO609_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6633864.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18997..19623)
                     /gene="dsbA"
                     /locus_tag="OO609_002485"
     CDS             complement(18997..19623)
                     /gene="dsbA"
                     /locus_tag="OO609_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6633865.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19640..20626)
                     /gene="srkA"
                     /locus_tag="OO609_002486"
     CDS             complement(19640..20626)
                     /gene="srkA"
                     /locus_tag="OO609_002486"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6633866.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20703..20972)
                     /locus_tag="OO609_002487"
     CDS             complement(20703..20972)
                     /locus_tag="OO609_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6633867.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21042..21626
                     /gene="mobA"
                     /locus_tag="OO609_002488"
     CDS             21042..21626
                     /gene="mobA"
                     /locus_tag="OO609_002488"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6633868.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21608..22135
                     /gene="mobB"
                     /locus_tag="OO609_002489"
     CDS             21608..22135
                     /gene="mobB"
                     /locus_tag="OO609_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6633869.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5480 a         5549 c         5306 g         5824 t
ORIGIN      
        1 tacgagcgca gcggtactga tgataaaggt gtagcataga tatttactcg gcgatttcag
       61 gatcttatct gaaatcatgc caccaatcgg tccaccaatc attttcagac agtattggtt
      121 aatgatgccg taagccccca ccagcgcaac gggcaataga tagatgtttt tcaggaatgg
      181 aatgaagaat gtcaggccac agtaaaccgc gtaaacgaag aagacgttga aagcgataag
      241 ccagatggtt ttgtctctca gcactgaggt catactggtg ttttgcgatg ctccgtcttc
      301 ttttttcacc tcaacggacg gtgcctcttc tttgtcattc aggacaaaga aaataataat
      361 gcctacggca atcacaataa gggaatagaa ccagatgcct gctttaaaac ctaataagcc
      421 actgccaaac caggtaaata ctgccaacgc agaaaatgcc accacggtat cgacaatgcc
      481 acgccctgtt tcgaagaagc caaacaaccg accttgttgt tcgctattgc ccaatcgact
      541 taccgacttg agcaagaccg gccagttcat catgtcgcaa gtaacgccaa atagcgccca
      601 gacaaagagg atgccccaat accccggcat tgtcgtcaaa taaacaccca gtaatcctgt
      661 cgcaatgagt gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa aataaataga
      721 cagaaaaaag cccactgtgg tgacaaatga gtttaccgac atagcattac caatttgacc
      781 attggtcaaa tggaaatatt cctgcatcgg gatataaaac gcatctttca gcgacggtaa
      841 tttataaatt gtaccgccac acagtgttaa tagactaaat aacgcccatt tctttttcgt
      901 gagcataaca tcccctaagc gatttttaaa atcactgttt taaaggcaat aagataccga
      961 ccattcatat ggaatggttt tattaactgc gtatgagtta aaacttatta gtggttcaga
     1021 agatggtttg cctcttgttt gatttgttgt agtacgttac gcacatgatt tatctgatta
     1081 attgcatctt ctttttcttt acgtaaccgc gcatccagta aatgattttc tggtaggggt
     1141 gtttcactca tctggcgata agggatccac ggattatcat cttcgtcttc aaagcggaac
     1201 gccgacgcat agtaatcaac ctcttcttcc aggccatatg ccgtcacctg cggctcatca
     1261 tcgccaaggc agataaggtg cgttaataac gctttgaatg gcatatcacc ctgacctgaa
     1321 atacaggctt tatgacccag gccaccctgt tctttaacga tcaaggcatc tttaatatgg
     1381 acctgggtaa tatgcggtgc catcgttttt aaggcgtcga tgggatgctc atttgcattg
     1441 atcatgttag caaaatcaaa cagtaaggaa agagactcca tctcactttc tttgaccagc
     1501 gacaccagct catgactttt taaatcttca tgctgctcaa tcgtaaaagt caggccgctg
     1561 tcctgatacg tttcccgtac ataggcaatg tcgttagcga taatcgataa tacgtcgcgc
     1621 aaattacctt cataacatgg ataaaaacgt acggacgttg ccccagtttt caacgcgatg
     1681 gcgacggctt cgtcaataga tgccttatct gaagcgctgg tttcaatatg aatatcaagg
     1741 ttcagacggc gggctttatc cccaaaggca gagagttctt tatcgtccat attaccaaga
     1801 gaaaaacgct cgccatccag aacatggatt ttgacgccac gtagattatt ttcactggcg
     1861 atatcaagta aatcagcagg caacactctt tccagccgca tatttaaatg aaaggcataa
     1921 gcatgtaaat aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc gttatttatt
     1981 gtcaccataa aacacctaca gattaatgat gaggtctacg gcctcaaatc gccaatattc
     2041 aatatctatc tggacaatat tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc
     2101 tggcataccc gcagaaccgc caagatactt gctgatatcg cccggcattc tgacgggttt
     2161 gaaaatcaat ttttttacta ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa
     2221 tgagtggttt tcaagttgtt caataaaacc cggagcaact tcaggattaa tatatgtttc
     2281 gtgatagaaa actttatgtc cttccagcgc cccccagccg gtgatgcgat agagttcagt
     2341 tgatggcgtc actgcaatca gtggcgcgag cgtctcggga atatcgctgg tacggttttt
     2401 ctcggtaaac ccccaagaag gctctcgccc ttgctcaatt gcggcccgct gaaagctcgc
     2461 cgacagctcc ggactgtaat taaatcgcgg ctgggtcacg aaccacccct tacgatcctt
     2521 acggaagatt ttggattctg cctcgaggtt caacaacgcc tggcgcagcg tcatacgttt
     2581 aatgcccagc agttctccca gctctctttc tgaagggagt tttccacccg gcgtggtgat
     2641 gccatctttc aaccaccgat ccagtttctc ttttgcattt tctacggtgg attgattctc
     2701 cgccattgtt taatcctttt ggtttaaacc agatgaagca tcttacgctt acgccaaaaa
     2761 agaaagcaat taacggtgta tgactttgca gataattgtg acaaagctct ctattgacgt
     2821 aatcacagac ttataaggga gatactcgca ttggtgtgcg ctcacgacag gtaaaaaaaa
     2881 acctgccatc gctggcaggt tttttatgac taaaaaacga aattaatcgt ctttcggtgc
     2941 gcggttggcg cggcgacgat cgttttccgt caggtgacgt ttacgaatac ggatagaggt
     3001 cggagtcact tctaccagtt cgtcatcatc gatgaactcc agagcttgtt ccagagtcat
     3061 gcggataggc ggaaccagaa caacagcttc gtcagtaccg gaagcacgca tgttggtcag
     3121 tttcttaccg gtcaggcagt ttacagtcag gtcgttagag cggctatgaa taccgataat
     3181 ctgaccttcg taaacttctg caccgtgacc gaggaacagc ttaccgcgat cctgcagacc
     3241 gaacagcgcg aacgcgacag ctttaccctg accgttagag atcagtacac cgttctgacg
     3301 ctgacccact tcgcccggac gtacgtcgtc gtagtggctg aaggtggagt acagcagacc
     3361 agtaccggaa gtcatggtca tgaactcaga acggaagcca atcagaccac ggcttgggat
     3421 cacgtagtcg agacgtacgc ggcctttacc gtctggattc atgtttttca ggtcgccttt
     3481 acgttcgccc agcgcctgca tcacagaacc ctgatgctgt tcttcaacgt ccagcgtcac
     3541 gttttcatac ggctcttgtt tacgaccgtc tatttcacgg aagattactt tcggacggga
     3601 taccgccagt tcgaaacctt cacgacgcat gttttcgata agaacagaga ggtgcagttc
     3661 accacgacca gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc gcagcgcaac
     3721 gttgtgtacc agttctttgt tcagacgatc gaggatctga cgagacgtta cgaacttacc
     3781 ttctttaccg cagaacggcg aggtgttaac gcagaagaac atagaaacgg tcggctcatc
     3841 aacggagagt gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt
     3901 cagttcgccc aggcccgtga tcgccacgat atcgccagct tccgccagat cggtttcgat
     3961 acgttccaga ccgaggtggc ccagcacttt accgactttc gcgttacggg ttttgccttc
     4021 gctatcgatg atagtaacct gctggttcgg cttcacttta ccacgcttga tgcggccaat
     4081 gccgataacg ccaacatagc tgttgtaatc gagctgagaa atctgcatct ggaacggacc
     4141 gtcaaggtca acgtccggcg caggaacgtg gtcaacaatc gcctgataca gcggggtcat
     4201 gtcttccgcc atatcttcgt ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac
     4261 gatcgggaag tccagctgct cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg
     4321 atccacaacc caatcaggac gcgcgccagg gcggtcaact ttgttgataa caacaatcgg
     4381 cttcaggccg taagcaaacg cttttttggt tacgaagcgc gtttgcggca tcgggccgtc
     4441 aaatgcgtca accaccagca gcactgagtc taccatggac attacacgtt caacttcacc
     4501 accgaagtcg gcgtgccccg gggtatcaac gatgttgata cggtaatcat tccatttgat
     4561 agcggtgttt ttcgcgagga tggtaatccc acgctctttc tccaaatcgt tggagtccat
     4621 cacgcgctct tgggtttcgg cacgagagtc gaacgtaccg gattgttgga gcagcttgtc
     4681 taccagggtg gttttaccat ggtctacgtg cgcgatgatg gcgatattac gcaatttttc
     4741 gatcacaact ttgcctcagg cattagaaat agcgcgttat tgtacacgga ttaatcgcac
     4801 tacaaaacag gatcacaaac atcctccgca aacaagtatt gcagagtccc tttgtgatcg
     4861 ctttcacgga gcataaaaag ggttatccaa aggtcattgc accaatatgg tgcttaatgt
     4921 ttccattgaa gcactatatt ggtgcaacat tcacatcatg gtgcagccct tttgcacgat
     4981 gttaagcatg ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt tcgctttatc
     5041 ttttttacgg cgacacggcc aaaataattg cagatttcgt taccacgacg acaatgacca
     5101 atccaggaga gttaaagtat gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa
     5161 gtgaagtttg ttgatttgcg cttcaccgat accaaaggta aagaacaaca cgtcactatc
     5221 cctgctcatc aggtgaatgc tgaattcttc gaagaaggca aaatgtttga cggctcctcg
     5281 attggcggct ggaaaggcat taacgaatcc gacatggtgc tgatgccaga cgcatccact
     5341 gcagtgattg acccgttctt cgccgactcc accctgatta tccgttgcga catccttgaa
     5401 cctggcaccc tgcaaggcta tgaccgtgac ccgcgctcta ttgcgaagcg cgccgaagat
     5461 tacctgcgtt ccactggcat tgccgacacc gtactgttcg ggccagaacc tgaattcttc
     5521 ctgttcgatg acatccgttt cggatcatct atctccggtt cccacgttgc tatcgacgat
     5581 atcgaaggcg catggaactc ctccacccaa tacgaaggtg gtaacaaagg tcaccgtccg
     5641 gcagtgaaag gcggttactt cccggttccg ccggtagact ctgctcagga tattcgttct
     5701 gaaatgtgtc tggtgatgga acagatgggt ctggtggttg aagcccatca ccacgaagta
     5761 gcgactgctg gtcagaacga agtggctacc cgcttcaata ccatgaccaa aaaagctgac
     5821 gaaattcaga tctacaaata tgtcgtgcat aacgtagcgc accgcttcgg taaaaccgcg
     5881 acctttatgc caaaaccgat gttcggtgat aacggctccg gtatgcactg ccacatgtct
     5941 ctgtctaaaa acggcgttaa cctgttcgca ggcgacaaat acgcaggtct gtctgagcag
     6001 gcgctgtact acattggcgg cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac
     6061 ccgaccacca actcttataa gcgtctggtc ccgggctacg aagcaccggt aatgctggct
     6121 tactctgcgc gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc tccgaaagca
     6181 cgtcgtatcg aagtacgttt cccagatccg gcagctaacc cgtacctgtg ctttgctgcc
     6241 ctgctgatgg ccggtcttga tggtatcaag aacaagatcc atccgggcga agccatggac
     6301 aaaaacctgt atgacctgcc gccagaagaa gcgaaagaga tcccacaggt tgcaggctct
     6361 ctggaagaag cactgaacga actggatctg gaccgcgagt tcctgaaagc cggtggcgtg
     6421 ttcacggatg aagcgatcga tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg
     6481 cgtatgactc cgcatccggt agagtttgag ctgtactaca gcgtctaata gttgaagttg
     6541 tactacccgg cgcacaacgc cgggatttag ttgccgtgga aactttcagc ccatctctgc
     6601 atgggctttt ttctccgtca attctctgat gcttcgcgct ttttatccgt aaaaagctat
     6661 aatgcactaa aatggtgcaa cctgttcagg agactgcttt atggcaacag gcacgcagcc
     6721 cgatgctggg cagatcctca actcgctgat taacagtatt ttgttaattg atgacaatct
     6781 ggcgatccat tacgccaacc ccgccgcgca acaactgctc gcccaaagct cccgcaaatt
     6841 gtttggtacg ccgttaccgg aactgttgag ctacttctca ttaaatatcg agctgatgca
     6901 agaaagtctg gaggcggggc aaggctttac cgataacgaa gtgacgctgg tcatcgatgg
     6961 tcgctcgcat atcctttctg tgacggccca gcgtatgccg gacggcatga tcctgctgga
     7021 gatggcaccg atggataacc agcgccgctt aagccaggaa caactacaac atgctcaaca
     7081 agtagctgcc cgtgatttag tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg
     7141 tttacgtggc gcggcgcagt tactcagcaa agcgttacct gacccatcac tactcgaata
     7201 taccaaagtg attatcgaac aggcggaccg gctgcgaaat ttggtcgacc gtctgttggg
     7261 gccgcagctg cccggtacgc gcgttaccga aagtattcac aaagtggctg aacgcgtggt
     7321 aacgctggtg tcgatggaac tgccggacaa cgtgcggttg attcgtgatt acgatcccag
     7381 cctgccggaa ctggcgcacg acccggatca aattgaacag gtcttgctga atattgtgcg
     7441 caatgcgcta caggcgctgg ggccggaagg cggtgaaatc attctgcgta cccgcaccgc
     7501 gtttcaactg accttacacg gcgagcgcta ccggctggcg gcgcggattg atgtggaaga
     7561 taacgggccg ggcattccgc ctcatttgca ggatacgctg ttttacccga tggtcagcgg
     7621 ccgcgaaggt ggcaccgggc ttggcttatc catcgcccgt aatttgattg atcagcattc
     7681 aggcaaaatt gaatttacca gttggccagg tcataccgag ttctcggttt acctgcctat
     7741 caggaaataa aggtgacgtt tatgcaacga gggatagtct gggtagtcga tgacgatagt
     7801 tccatccgtt gggtgcttga acgtgcgctc gctggagcgg gtttaacctg tacgacattt
     7861 gagaacggcg cggaagtact ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca
     7921 gatatccgta tgccgggaat ggacgggctg gcgctgctca agcagattaa acagcgccat
     7981 ccaatgcttc cggtcatcat tatgaccgca cattccgatc tggatgctgc cgtcagcgcc
     8041 tatcaacaag gggcgtttga ttatctgccc aaaccgtttg atatcgacga agcagtggcg
     8101 ctggttgagc gcgctatcag tcattaccag gaacagcagc agccgcgtaa tattcagctt
     8161 aacggcccaa cgaccgatat catcggcgaa gcgcaagcca tgcaggacgt gttccgtatt
     8221 atcggtcggc tttcgcgttc ttctattagc gtgctgatta acggcgaatc cggcaccggt
     8281 aaagaactgg tcgctcatgc cctgcatcgc cacagtccgc gcaccaaagc gccgtttatc
     8341 gcgctgaata tggcagctat cccaaaagat ttgatcgaat cagaactgtt tggccacgag
     8401 aaaggcgcgt ttactggcgc gaataccatt cgtcaggggc gttttgaaca ggccgatggc
     8461 ggcacgttat ttctcgacga aattggtgat atgccgctgg atgtgcagac gcgtttgctg
     8521 cgcgtgctgg cagacggtca gttttatcgc gttggcggct atgcgccggt gaaagtggat
     8581 gtgcggatta tcgctgccac tcaccagaat cttgaacagc gggtgcagga aggtaagttc
     8641 cgtgaggatc tgttccaccg cctgaacgtt atccgcgttc atctgccgcc gctgcgcgaa
     8701 cgtcgggaag atattccccg tctggcgcgc cattttttac aggttgccgc gcgcgaactg
     8761 ggcgtagaag cgaagttgct gcatccggaa accgaagctg ctctgacgcg tctggcgtgg
     8821 ccaggcaacg tgcgccagct ggaaaacacc tgccgctggc taacggtgat ggccgccggg
     8881 caggaagtgt tgattcagga tttgcccggc gaactgtttg aatcaacggt tgcggagagt
     8941 acttcgcaaa tgcaaccgga cagttgggcg acacttttag cgcagtgggc agacagagcg
     9001 ctgcgttccg gtcatcaaaa tctgctttcc gaagcgcagc cagagctgga gcggacgtta
     9061 ctgacgaccg cgttgcgaca tacgcagggg cataaacagg aagcggcgcg gctactcggc
     9121 tggggccgca acactctgac gcgtaagtta aaagagctgg ggatggagtg attcacggct
     9181 tgtgtgtaaa gattgattat tgagcgcaaa ttgctggtat tttgcgcttt actgttccga
     9241 taagttcagt atgatcttgc ccggaaaacg gggagagtca ttatgctgga atcaataatt
     9301 aatctggtat cgagtggcgc agttgacagc cacacaccgc aaactgccgt tgctgccgtg
     9361 ctgtgtgccg cgatgattgg gctgtttagc tgaagctcgt tgtttatgcc ggatgcggag
     9421 aaagtcgtag gcctgataag acgcgaccag cgtcgcatca ggcatcatgc tcagatgcct
     9481 gatgcggcgt aaacgcctta tccggcctac aaaagcaata atttcaacag gttttgttgc
     9541 ccagcgcatc aggcaatctt gttctcagca cattgatggc gctttcgttt tctactttgt
     9601 aaacgaagcg ccattcactc tttaaatcac ccgtgagaac tgctgcatcc gcgctttctg
     9661 gcgcagatag gtatcaaagc acatgcaaat gttgcggatc agcaagcgac ctttcgccgt
     9721 cacctgtatt cccttctcat ccacatccac cagcccatct tttgctaacg gggcgagcag
     9781 cttgagatct tccgcaaagt aatcagcgaa gtgcaaatcc cactgtttct caagaggggc
     9841 gtaatccaga cggaagttgc agatgagcga cttaatcaca tcgcggcgaa tacagtcatc
     9901 acgcgttagc gcaataccac gccacagcgc attgccttgt tcatacactt gctgatagta
     9961 ctgcttcaac tctttctggt tctgcgcgta gcagtcgcca atcatgctga tggcggaaac
    10021 gcccatcccc agcagatcgg tatcgccctg agtggtgtag ccctggaagt tacgatgcag
    10081 cacgccttca cgctgggcca ccgccagctc gtcatcagga cgggcaaagt gatccatacc
    10141 gataaactga tagcccgatt gcgtcaggaa ggcgatggtt tcctgcagga tatcgagttt
    10201 ttgctgcgga ctcggcaggt cagcatcttt gattttgcgc tgagcagcaa aaatggtcgg
    10261 cagatgcgcg tagttaaaaa cgctcagacg atcggggttc agctccgcca cacgtttcag
    10321 ggtaaaggcg aaactctccg gcgtctgttt cggcaggccg taaatcaggt cgatgttggt
    10381 ggaggtaaaa ccaatctcac gcgcatggtt aagcagtgca aagatgaact cttcatcctg
    10441 ctcgcggtta accagacgtt gcacttcttt gttgaagtcc tgcacgccca tgctcaggcg
    10501 attaaagcct tcggcgcgta aatgatcgag tacatccagt tcgatttccc gcggatcgac
    10561 ttcgatcgaa atctccgcat cggtattgaa atggaagttt tcgcgcagca gcttcatcag
    10621 acggctgatt tgcgctttat tcagatacgt cggcgttccg ccgccccagt gcaattggct
    10681 gacgtgacgc ccggcgaaca gcggtgcacg atggacgatt tcttgctcca gcgcgtccag
    10741 atactgatcg gccttgtgct gctggcgagt aacaatctta ttgcaaccgc agaagtaaca
    10801 aagcttatgg cagaacggga tatgtacgta gagagataat ggacgctcag gatagcgcgc
    10861 cacggcttgt aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg cggtcgggta
    10921 cgaggtgtat cgtggcccgg aatagttata tttctggatc agggccagat cccagtcgat
    10981 ttgctgtaca gacatgctca ctccttccga tggcgtctct ggcggctacg gcgtaccggc
    11041 ggatgcccgt ttcgggtcat cagccggttg cgcagccact tttgtcgccg cgacaaccgc
    11101 tgtagtttaa cgaataacca caccagataa catataaccg gaagggttat cagcagaacg
    11161 ggaaggccca cggcggcaat ccgttagttg ccccgcagca gacgcatcat gtcttcttgc
    11221 ttctcgtctt cttcctcttc ttcgtcatcg tcataagaga ggccgagttt ctgcatcaac
    11281 tcatcaatac gatccagttt agcatccacc caggattgct cttcggcgct cagggtttcg
    11341 cctgcttcca gacgttccag cagcgcatcc agacactcat ccgtttccag taattccaac
    11401 tccgcctgcg gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt ggtgactttt
    11461 tcagtcactc ccaatggaat aggggtttta ctgccaatac gtggatcttt tggtgcgttc
    11521 tggcctttgc tgcctgacgt ggtgtttccg cccgctgcgc ggctgcccgg cgcatgacca
    11581 cgacgttttt tctgacgctt acggtcacga gcttcctgat ccagctcctc gcgtgttttt
    11641 cgacgcgctt ttgcatgacc tttgctgcgt gagtttgaag atgatggttt cataatggtt
    11701 cgtcttttag ccgttttatt cagtatagat ttgcggcgga atctaacaga aagcaagcaa
    11761 agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg actcataacc cttaacgggt
    11821 cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc attagccgga acaatccgtg
    11881 ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt cttcctgaca atcctctgtc
    11941 ttcgcctcct ggcgctcctg accctcatcc tgagtcattg ttcctgttag cgtcctcgct
    12001 ctatgggtgc tactttacgc ctttgcttta aacaaacaat caaccgccat cagtatttgc
    12061 acattttcag agttttatat aaaagcaatt cactgattta tatagcttat ttgacatgca
    12121 tcgcagaatt ttctctgcta tttcgcataa agactatcgg caatgtctca caaagcgtat
    12181 ggcttttact tacaacgctc acgccgaaaa atccgccttt tattatggtt caggtataat
    12241 cgccacaaag ctttgactga cggagacgac cgctttgact aatttgaatt atcaacagac
    12301 gcattttgtg atgagtgcgc ctgatattcg ccacctacct tccgataccg gaattgaagt
    12361 ggcttttgca ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc tgactaacca
    12421 gaaaagcctg gctcgtacct caaaaacccc agggcgcacc cagcttatca acctgtttga
    12481 agtggctgac ggcaagcgtc tggttgactt gcctgggtac ggttatgcgg aagtcccgga
    12541 agagatgaag cgcaaatggc agcgtgcgct cggcgaatac ctcgaaaaac gtcagagcct
    12601 gcaaggtctg gtggtgctaa tggatattcg ccatccgctg aaagatttgg atcagcagat
    12661 gattgagtgg gcggtagaca gcaatatcgc cgttctggtg ctgctgacca aagcggacaa
    12721 actggcaagc ggcgcacgta aagcgctatt gaatatggtg cgtgaagctg tactggcgtt
    12781 taacggtgat gtgcaggttg aaacgttttc ttcgttgaag aaacaaggcg tagacaagct
    12841 gaggcagaaa ctggatacct ggtttagcga gatgcagcct gtagaagaaa cgcaggacgg
    12901 cgaataattt tcttgcctta atgcttgtgc cggatgtggc gtatccggcc cgtaaattca
    12961 atagattaca attctaccgt tggcctgata aacatagcgc atcaggcatt acggatcttt
    13021 tctttcgccc aataaaaaac gccccagtca ttactgactg gggcggctaa aatattcagc
    13081 caaatccgat tacgtgaagt aaaaggtctg aaagatagaa catcttacct ctgtacccta
    13141 cgcgactaac tttactcttt tttgttcagt tcagaaagca ctttttgtaa ttttttttca
    13201 gttgttgcat aggaatttct aatagccatc acaaaacgtg acagcttatg ttgcttactt
    13261 acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg atcccagttt tcgccactcc
    13321 ccacttccac cagcaacggc acatccagac gggtacagtt ttccatcagt tgatgaatct
    13381 gcttcgcgac ggcatcaaca tcatctttat gaacttcaaa taccagttca tcgtgtacct
    13441 gcatgatcat acgtacacgc ggttgctcag cctgtaacca cgcatcaacg gcaatcatcg
    13501 cccgtttgat aatgtcggcg gcggttccct gcattggcgc gttaatggct gcacgttcag
    13561 ccgctgcacg acgagcacca ttgctggatt tgatatccgg cagatacaga cggcgtccgt
    13621 ccagcgtttc aacgtagccc agctctttcg cctgagcacg ggtgcgttcc atatactcca
    13681 gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt ctgcgcttct ttacgtggaa
    13741 tgttcaattg ccgcgccaga ccgaaagcac tcatgccata aatcagacca aagttgatcg
    13801 ctttcgcgct acggcgttgc tcgctggtga cggtttccag tggcaaacca aacacttctg
    13861 ccgccgttgc ccggtggata tcttttcctt ccgcgaatgc ggtcagcaag cctttatcac
    13921 gcgagagatg cgccataatg cgcagttcaa tctgcgagta gtccgctgag acaatcacat
    13981 aatcctctgg cgcaataaac gcctggcgga tacgacgacc ttcttcgtta cgcaccggaa
    14041 tgttttgcag gttaggatcg gttgacgata aacgtcccgt tgcagttact gcctggtgat
    14101 aagaggtatg cacacgcccg gttttcgggt tgatcatcag cggcagcttg tcggtgtaag
    14161 tcgacttcag cttcgccaga ccacgatact ccagaatcac ttttggcaac ggatagtcca
    14221 gcgccagttc ttccagtacc tcttccgacg ttgacggcgc gccacccggc gttttcttca
    14281 gcggtttaat accctgtttt tcaaacagaa tggtttgcaa ctgcttggtg gaagaaaggt
    14341 taaattcttc acctgcaatt tcatgcgctt tcttttccag ctcagccaga cgcaaggtga
    14401 gctcttcaga atgattgtgc agcactttcg gatcgatctt cacaccgtta cgttcaatgc
    14461 gtgaaagcac cggcaccagc ggcatttcga tattctcgaa gacgttcaac ggccctttgt
    14521 gtttctgcag atccggccac attttcagat gcaactgcaa ggtgacatct gcatcttcgg
    14581 cggcgtaacg tccggcctct tcgagggcaa tctggttaaa ggtcagttga tttttgcctt
    14641 tacccgcaat ctcttcaaaa gtgatggttt tgtgcttcaa ccaacgttcc gcgaggctgt
    14701 ccatatcgtg acgcccggca acgctattga gaatgtagga ctccagcatg gtatcaaacg
    14761 caatcccacg cagttcaatg ccgtaattcg ccagaatacc gcgatcgtat ttcaggtttt
    14821 gcccgacctt cagcgccttt tcatcttcca gcagcggttt tagcaactcg agtgcacgct
    14881 cgtgagagat ttgatcgggc gcatcaagat aatcatgagc aaccggaata tatgccgcta
    14941 cgcctggctc gatagcaaaa gaaagcccga ccaggttagc agagatgtta tcaaggctgt
    15001 cggtttcggt atcaaatgca aataccggcg ctttttccag cttcgcaatc cacgctttca
    15061 gtgtttcttc atcaaggata gtgacgtagt tgtcataaga aatcaccgtt gccgtcactt
    15121 ctggcgcttc gtctgcaaca ctggtttcct gaggcttcgc ggctgttttt gcccctttgg
    15181 cctgtaacca tttgcccact tcgacatcag cagtccagcg tttgaactca tactttttga
    15241 acagccccaa caactcttcc gctgccggtt gctgcacttc cagttgttca caggtcagct
    15301 ccagttcaac atcggtttta atcgtcgcca actggtatga gagataagca acttctttgt
    15361 tttgctcgag cttcgctgcc attgttttcg cgccacggaa gctcaaccca gcaatttttt
    15421 ctggctcggc atacagcgta tccagcccgc caagaccttg cagcaatgcc tgcgcggttt
    15481 tttcaccgac gcccggtacg ccaggaatgt tatcagagga gtcacccatc agcgccagga
    15541 agtcgatgat caattctggc ggcacgccgt acttattcac cacctcttcc ggtccgagga
    15601 tggtattcgt catggtattg ataagcgtaa tatttggcgt caccagctgc gccatatctt
    15661 tatcgccagt gctaatcagc accggacgcc cggctttttc ggcttcgcgc gccagagtac
    15721 cgataacgtc gtccgcttct acgccagaaa ccgccagcag cggcagtccc atcgctttaa
    15781 ccatcgcgtg cagaggttcg atttgtgctc gcagatcgtc cggcattggc gggcgatgtg
    15841 atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc cttggcgtca aagaccaccg
    15901 ctgcatgcgt cggtttatat tgcatgatca gactgcgcag catgttgagg acaccataca
    15961 tcgcaccggt cggctcgcct gcgctgttag tcagcggggg aaacgcgtga tatgcgcgat
    16021 aaagataaga tgaaccatct acaaggataa gtggattttg ggggatctga accataatgt
    16081 ccgtgcctgt ttatcagatt atgggtaaag gatgccacag acaggatgaa aacatgagtt
    16141 tttcaccgct tttcgtgaaa atcttttgta gatctgctgg atcgctcgca aatcgtcatg
    16201 tggataactt tgtgcataaa ttatttacat cgcttttaat ttattatcat gctttttaca
    16261 gcatgatgtt tgtctttgtt tttcatgcag ttaaaaataa atccatccct aataattgag
    16321 ataatgggat tttttctaat gtggatagat atgaattatt tttctcctta aggatcatcc
    16381 gttatttggg tcattttctg ctaaaatctg cgccttgcgc ttctttagtg cactgtctat
    16441 ggctaacctt ttgaataaat tcattatgac gagaatactc gctgcgataa cccttttgct
    16501 gagtatcgta ttgaccattt tggtcactat tttttgttcc gtcccgatca tcattgccgg
    16561 gattgtaaaa ctattgctac ctgtgccagt catttggcga aaggtttcgc gtttttgtga
    16621 ttttatgatg tattgctggt gtgaaggtct ggcggtatta ctgcatctga acccacacct
    16681 gcaatgggaa gttcacgggc tggaagggtt aagtaagaag aactggtatc tgcttatttg
    16741 taaccaccgt agttgggcag atattgtcgt actgtgcgtg ctgtttcgta agcacattcc
    16801 gatgaataaa tatttcctca agcaacagct ggcctgggtg ccatttcttg gcctggcgtg
    16861 ctgggcgctg gatatgccgt ttatgaagcg ttattcccgc gcttatttat tacgtcatcc
    16921 ggagcgacgt ggtcaagatg ttgaaaccac tcggcgttct tgtgaaaagt ttcgcctgca
    16981 tcccaccacc attgtcaatt tcgttgaagg ctcccgtttc acgcaagaaa aacatcagca
    17041 aacccactcc acttttcaaa acttgttgcc accaaaggcg gcaggcattg cgatggcgct
    17101 aaatgtactg ggtaaacaat tcgataaact gcttaatgtt acgctgtgtt atccggacaa
    17161 taaccgtcag ccgttcttcg atatgttaag cggtaaactg acgcggattg tcgtacatgt
    17221 ggatttacag cctattgccg atgagttaca tggcgactac atcaatgaca aaagctttaa
    17281 gcgccatttt cagcaatggc tgaactcatt gtggcaggaa aaagaccggc ttctgacgtc
    17341 tctcatgtca tcacagcgtc agaataagta agttttactc gcctctgaat gagcagaggc
    17401 gagtgagtat tttaatgaac aaaacgtccg gcacgagaca taaattcttc ttcgctcatc
    17461 tcctgtccgt taaaaacaac tttacccggt gtataacgca gctgcagcga ggcggtattg
    17521 ttgtcaatcg tgattaactg aaacatctga cctaatgtca tcatcccgct gatttgttta
    17581 tcagcctgct tttgcgcttt ttccgcatcc attccttcag ataaattaag ctgttttgcg
    17641 gtttctgtga cgacattaag cggtaacttc acatcaaaat tgagcgattt gatatctttg
    17701 tttgtggatg atgaagattt ggctgggtcg gcaatactga tatccagatt ggcatttagt
    17761 tcgccgagtg cgttcttcca tcttaccggt tgtttaatgg tcggctcact tttttgtaat
    17821 aacggtaaat actctttgaa caatgcagca ttcacttctt gcagagcaac ttcatcgttt
    17881 gctaactcag ggtgtgcagc aagctgcttc tgcatcgcaa tgttatactg gatgataaat
    17941 tggcgcactg ctgagggatc aatagattca gcaatcaatg accactcacc actaccgagt
    18001 tgctgattcc cttttgttaa cttatcaagt tcgtaggtca gattgacatt gatataatct
    18061 ttgtctttaa cgcgatccag cctggttttt gcaacgaaag catcgatttg ggcaacgtct
    18121 tccccgtgat tgataatgtt aatctgatta aatttcgact cgctttcacc aaccggaaat
    18181 ttcttttctt ctaatcgcgc cagagaatca atggtaaaac tttttgccgt tactttgttc
    18241 attggtgata attgcagaac aatatcttcg actttgcctt caatatccag gttagacaat
    18301 gaactaccat caccattaaa agtaaactgg ccctcaccaa aagaaaattt tccatactct
    18361 gcaggggcaa catctaatgt cgtagagaat gagccaccaa acgcgaatcg caatgtcgct
    18421 tccacgggcg atttattttt cgcggcgata aatagcggtt gtgtcagttc attattcact
    18481 aagttcactt ttgccgctgc cagtgctggg atgacattac catgcattaa cattgtgatg
    18541 ggcaacggac cgtgctcaac atccacatca aatacaactt tctggcctgg cttgatattg
    18601 agatcgggtg caccgttatc gaatgtcatt tgcatttgaa aacgggaatt aaaaaagcct
    18661 ttgtcaaagt ttttataact taacgtcata tcgtaggcat gttcaccttt ctttttcgca
    18721 tcgttaaaat ctttaataaa cttttcgata ccaggctgaa tttgcgtacc tgtgtaccat
    18781 gtgccaccac cccagattac ggctaacgca acaataacac ctgtagctga cttacgtatc
    18841 atataaatat tccctgataa aacagcaaaa ctttgaatat ccacttatgc tgaaataatg
    18901 tccagcggca ggatgcatta tcagaatgta tttatattta ttaaataata aaaaaagccc
    18961 gtgaatattc acgggcttta tgtaatttac attgaattat ttttcctcgg acagatattt
    19021 caccgtatca gcatactgct gaacaaaaac atccatattg ctggtatcca taccctgcgg
    19081 attcagctga tatttaccgt taacaaacat cgccggaaca ccacgcaatt gcacatcagc
    19141 tgcggctttt tcctgctgag cgaccagaga tttcaccacg aagctgttcc acgccgcgtc
    19201 gtactcttca cctttaatac ctgcattgat aaatacatcg cggatatcag atgctgaacg
    19261 aatggtctgg gttttctgta cgccttcaaa cagcggaaca gtcactttgt cttccacgcc
    19321 cagcgccatc gccacagccc atgcctgagt cagatctttg cccaggtcac cacccatgaa
    19381 gttgacgtgg tatttagtca tcttcacgcc ttccggcagt tttttcttca cattatcaga
    19441 aatatgcaga acttcttcaa actgatagca gtgcgggcag aagaaagaga aaaactccag
    19501 cacttgcggc gcgccagcta ccggtttttc cagggtagtg tactgtttac catcttcata
    19561 ctgcgccgcc gatgcgctaa acgctaaaac taaaccagcc agcgccagcc aaatcttttt
    19621 catgatctac tctctccgat taatacatag gtgttaattg caaagggggt tcttgtagaa
    19681 cttttgcctg ttctataaaa gtcgccgtct gtcgtagcca gtaatcttcc ccggttaacc
    19741 acgggaaatt tttcgggaac gcgggatcag cccaatgccg cattagccag gcaagataat
    19801 aaaccaaacg catggcgcgt aaaggttcaa tcagtccgat ttcagcggtg tcgaactcgc
    19861 taaattcttc ataagcttca ataatagttt ccagttgcat ccgctgctgg gctttatcgc
    19921 cattgagcaa catccacaaa tcctgaatag ctggaccatt acgtgcatca tccagatcaa
    19981 caaacattgg accatcgcgc cagagaatat tcccggcgtg gcagtctcca tgtagccgca
    20041 gaacggtgaa atcttcccgc cagtgtgctg taacggcggc gatcagctca tctgtcgctt
    20101 tcagaaatgc cgctttcaac ccggaaggta tcagtgtagc gtcctcaaac agcttgcgag
    20161 gctcaatgag atattcattc agaccgatgg tcgggcgatg aataaaaagc tgtttgcgcc
    20221 ccgtctggtg catacgccct aaataacgcc caaccgcttc catctgatcg atattatcag
    20281 cttcgaactg gcgaccaccg acgcttggaa aaacagcgaa ataaaatccc tgatgattca
    20341 ataaagtctg accgttaaag gccacaggtg ctgcgaccgg aacttcatca tttaccagct
    20401 gcaacgcaaa ttgatgttct tcgaggattt gatcggctgt ccaacgttca gggcgataaa
    20461 atttgacgac aaaacgtcga cgctcttcgt cctgaaattg atagacacgg ttttcatagc
    20521 tgttaagcgg ggtaagaccg gaatccaccc ggatcccttg cttaaacaga gcgtccatga
    20581 tggtatccgg gtgtagtgtc tggaaagtaa aagcgctgtt gttcatccca tcatccggaa
    20641 aacattacga atgatgaagg atatcatttc acgatgattt tggtggcgcc gcttacaagc
    20701 ttttactctt taattacgcc gcgagcgcgt aacagcgccg ttttgaagtc ttcttcataa
    20761 tctttctgca aacccgggat caccgcatct ttcgcggaat cacgcatttt caggtgatag
    20821 atcagaatgt catccgtcaa atccgccagt tcgccgtcaa aacctgactc tttcgccagt
    20881 ttctgcaaaa attgcaacaa gttaaagtct ggctcttttt gccaggctgg ctggaggagt
    20941 tcaataactt cattcagacg tttacatttc atagtaatgc tccttactct tgagacagac
    21001 acgttagcag ggtcaatccc acaataaaag aggcgatatc ggtgaatcta atgacgacga
    21061 taacaggcgt tgtgctggca ggcggtaaag ccagacgaat gggcggcgta gataaaggat
    21121 tgctggactt aaatggcaaa ccgctgtggc aacatgtcgc tgacgcgctt aagacgcagc
    21181 tctctcacgt cgtggttaat gctaatcgtc atcaggaaat ctatcaggca ggcggtctga
    21241 aagtgattga agattcactg gcggattacc caggccctct ggcaggaatg ctttcagtaa
    21301 tgcagcagga agctggtgag tggtttttgt tttgtccgtg cgatacgcct tacattcccc
    21361 atgatttagc cgcccggctt aatcatcagc gcaaagatgc gcctgtcgtg tgggtccatg
    21421 acggtgaacg cgatcacccg actattgctc tggtaaaccg cgctattgag cctttattac
    21481 tggaatatct gcaagcagga gaacgccggg taatggcatt tatgcgtctg gctggcggtc
    21541 atgcggttga tttcagcgat cataaagatg catttgttaa cgtgaatacg ccagaggagc
    21601 ttgcccgatg gcaggaaaaa cgatgatacc gttactcgcc tttgccgcgt ggagtggtac
    21661 cggaaaaact acgcttttga aaaaactgat cccggcatta tgcgccagag ggatccgtcc
    21721 ggggctgatt aagcatacgc accatgatat ggatgttgat aagccaggca aagatagcta
    21781 tgagctgcgc aaggctggcg cggtacaaac catcgttgcc agccagcaac gatgggcctt
    21841 gatgacggaa acaccagacg aagaagagct ggatctacat tttctcgcaa gtcgaatgga
    21901 tacctcaaag ctggatttga ttctggtcga agggtttaag catgaagaga tcgcaaagat
    21961 tgtgctgttt cgcgatggag ccggacatcg acctgaagaa ttagtgatag acaggcatgt
    22021 tattgctgta gccagtgatg tgccgcttaa tcttgatgtc gcattactgg atattaatga
    22081 tgttgagggg ctggctgatt ttgtggtgga gtggatgcaa aagcagaacg gataagcaag
    22141 agtaggctgg atcagacgc
//
LOCUS       DAJTYH010000065        21880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTYH010000065 DAJTYH010000000
VERSION     DAJTYH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..2106
                     /locus_tag="OO609_002490"
     CDS             <1..2106
                     /locus_tag="OO609_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6633870.1"
                     /translation="ERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNL
                     GPWRLRNNSTWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRG
                     VQLASDDNMLPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYST
                     SSSGDLLVEIKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFA
                     QTTLQWGGPWGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEH
                     KGQSYRFLYAKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPM
                     WSRYYNLFYTKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKD
                     LSLGVSWNYSKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDN
                     EGHITQNLGLTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSD
                     NGSQQQLNYALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGY
                     TVVPYATSYRENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKT
                     SKQGIPLRFGAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISY
                     QLTEQQINSAITRMDAICR"
     gene            2097..3080
                     /locus_tag="OO609_002491"
     CDS             2097..3080
                     /locus_tag="OO609_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6633871.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3178..3718
                     /locus_tag="OO609_002492"
                     /pseudo
     CDS             3178..3718
                     /locus_tag="OO609_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3726..4241
                     /locus_tag="OO609_002493"
     CDS             3726..4241
                     /locus_tag="OO609_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6633872.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4207..4944
                     /locus_tag="OO609_002494"
     CDS             4207..4944
                     /locus_tag="OO609_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6633873.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5055..6065
                     /gene="pyrD"
                     /locus_tag="OO609_002495"
     CDS             5055..6065
                     /gene="pyrD"
                     /locus_tag="OO609_002495"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6633874.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6239..6781
                     /gene="zapC"
                     /locus_tag="OO609_002496"
     CDS             6239..6781
                     /gene="zapC"
                     /locus_tag="OO609_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6633875.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(6778..7887)
                     /gene="ycbX"
                     /locus_tag="OO609_002497"
     CDS             complement(6778..7887)
                     /gene="ycbX"
                     /locus_tag="OO609_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6633876.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8131..10239
                     /gene="rlmKL"
                     /locus_tag="OO609_002498"
     CDS             8131..10239
                     /gene="rlmKL"
                     /locus_tag="OO609_002498"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6633877.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10251..12158
                     /locus_tag="OO609_002499"
     CDS             10251..12158
                     /locus_tag="OO609_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6633878.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12288..13541
                     /gene="pqiA"
                     /locus_tag="OO609_002500"
     CDS             12288..13541
                     /gene="pqiA"
                     /locus_tag="OO609_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6633879.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13546..15186
                     /gene="pqiB"
                     /locus_tag="OO609_002501"
     CDS             13546..15186
                     /gene="pqiB"
                     /locus_tag="OO609_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6633880.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15183..15746
                     /gene="pqiC"
                     /locus_tag="OO609_002502"
     CDS             15183..15746
                     /gene="pqiC"
                     /locus_tag="OO609_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6633881.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16002..16169
                     /gene="rmf"
                     /locus_tag="OO609_002503"
     CDS             16002..16169
                     /gene="rmf"
                     /locus_tag="OO609_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6633882.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16239..16757)
                     /gene="fabA"
                     /locus_tag="OO609_002504"
     CDS             complement(16239..16757)
                     /gene="fabA"
                     /locus_tag="OO609_002504"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6633883.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(16826..18586)
                     /locus_tag="OO609_002505"
     CDS             complement(16826..18586)
                     /locus_tag="OO609_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6633884.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            18771..19223
                     /gene="matP"
                     /locus_tag="OO609_002506"
     CDS             18771..19223
                     /gene="matP"
                     /locus_tag="OO609_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6633885.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19299..20345)
                     /gene="ompA"
                     /locus_tag="OO609_002507"
     CDS             complement(19299..20345)
                     /gene="ompA"
                     /locus_tag="OO609_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6633886.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(20701..21210)
                     /gene="sulA"
                     /locus_tag="OO609_002508"
     CDS             complement(20701..21210)
                     /gene="sulA"
                     /locus_tag="OO609_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6633887.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21429..21839
                     /gene="sxy"
                     /locus_tag="OO609_002509"
                     /pseudo
     CDS             21429..21839
                     /gene="sxy"
                     /locus_tag="OO609_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5580 a         5150 c         5736 g         5414 t
ORIGIN      
        1 gaacgttggg atgaagggat caacgcgcta ttactgggat attcgtttag cggggctaac
       61 agtattcata gcagcgcaga cagtgattct ggcgacagct attttctgaa tttaaacagt
      121 ggcgttaatt taggcccatg gagattacgc aacaattcaa catggagtcg cagtagtggc
      181 caaaccgcag aatggaagaa tctcagcagc tatttgcagc gggcggttat tccactaaaa
      241 ggcgaactga ccgtaggtga tgattatact gcaggcgatt ttttcgatag tgtcagcttt
      301 cgtggtgtgc agctggcgtc agatgacaac atgctgccag acagcctgaa agggtttgcg
      361 cctgtggtgc gtggtatcgc caaaagcaat gcccagataa cgattaagca aaatggttac
      421 accatttacc aaacttatgt atcgcctggt gcttttgaaa ttagtgatct ctattccacg
      481 tcgtcgagcg gtgatttgtt agttgaaatc aaagaagcgg acggcagcgt caatagctac
      541 agcgtaccgt tttccagcgt gccattactc cagcgtcagg ggcgaatcaa atacgcggtg
      601 acactggcga aatacagaac caatagtaat gaacagcagg agagcaaatt tgcccagacc
      661 acgttgcagt ggggcggacc gtggggaacg acatggtatg gtggtggaca atatgctgaa
      721 tattaccgtg ccgccatgtt tggtctggga tttaaccttg gcgatttcgg agcaatttcg
      781 ttcgatgcga cccaggcgaa gagtacgctg gcagaccaaa gcgaacataa aggtcagtca
      841 tatcgttttc tgtatgccaa aacgctcaac caattgggca ctaattttca attgatgggc
      901 tatcgctatt cgacgtcggg tttctacacc ctttccgaca ccatgtataa acatatggat
      961 ggctacgaat ttaatgacgg tgatgatgaa gatacgccga tgtggtcgcg ttattacaat
     1021 ttgttttaca ccaaacgtgg caaactgcag gtcaatatct cccagcaatt aggcgagtac
     1081 ggttcgtttt atttaagtgg tagccagcaa acttactggc ataccgatca acaggatcgg
     1141 ctattacagt ttggctacaa cacgcaaatt aaagatctct cgctgggggt ttcctggaac
     1201 tacagtaagt cccgtggtca acctgatgct gatcaggtgt ttgcactaaa tttttccctg
     1261 ccgctcaatc tgttgctccc cagaagtaat gatagctata ccaggaaaaa aaattacgcc
     1321 tggatgacct ctaacaccag tatcgataac gaagggcaca ttacacaaaa cctgggttta
     1381 acggagacac tactcgatga cggtaacctg agctacagcg tgcaacaggg atataacagc
     1441 gaggggaaaa cggctaatgg tagcgccagc atggactaca aaggggcgtt tgcagatgcc
     1501 cgagtgggct acaactacag cgataacggc agtcaacaac aactgaacta cgctctttca
     1561 ggcagtttag ttgcccattc acagggcatt accctggggc aatcgctggg ggaaactaac
     1621 gtgctgattg cagcaccagg cgcagagaat actcgtgtgg cgaacagcac cgggctgaaa
     1681 actgactggc gcggatatac cgttgtcccc tatgccactt cttatcggga aaatcgaatc
     1741 gcacttgatg cggcatcgtt aaaacgtaac gtggatcttg aaaatgcagt agtcaacgtg
     1801 gttcccacca aaggggcgtt ggttctggcg gagttcaatg cccatgcggg agcaagggta
     1861 ttaatgaaaa catcaaagca gggtataccg ctgcgttttg gcgcgatagc gacgctggac
     1921 ggcatacaga ctaatagcgg cattattgat gatgatggct cgctctatat gtccggtttg
     1981 cccgctcagg gagcgataac tgtacgctgg ggcgaagctc ccgatcaaat ttgtcatatc
     2041 agttaccagc ttaccgaaca acaaattaac tctgcgatta cgcgaatgga tgccatatgc
     2101 agataatctt tggagaaaaa tgcgtgttat tactacgact attttttgcc gccgtcttaa
     2161 tgctatggtg cgctcaaact gctgcttata tcgggcagtg tcataccact caggggaatc
     2221 cgtatattgg cgtcaatttt ggcgttaaaa ctctggatga agaagaaaat acggctggag
     2281 tagttaaaga caaattttat cagtggaacg aatcgaatga ttattatgtt tcctgtgatt
     2341 gcgataaaga caatgtcaga agtggccgat gggcattcgc cgcggattca ccgttagtct
     2401 atttaggcga caactggtac aaaattaatg actatcttgc cgccaaagtt ttattgcagg
     2461 ttaaaggcag ttctcctact gcggttcctt tcgaaaacgt gggcacaggg gcagatacac
     2521 gatggcatat ttgcgatccc ggcggtcaac gtttaggtgg ccagggggct agcggtaata
     2581 gcggtagctt ttccctgaaa atattgcagc cgttcgttgg ttcggtcgtc attcctccta
     2641 tggcgctggc gcgattattt gaatgctaca acatacccgc aggtgattcc tgcacgacta
     2701 ctggtacacc ggttttagtg tattacctgt ctggtacgat caattcactt ggctcatgtt
     2761 ccgtcaatgc cggagaaaca atcgaggttg atctgggcga cgtatttgcg gctaactttc
     2821 gtgttgtagg gcataagcct cttggggcca gaacggcaga acttgcaatt ccggtcaggt
     2881 gtaacacggg aaacgcaggg ttagttaacg tcaatctgag tctgacggca acctcagacc
     2941 ccagctatcc ccaggcgatt aagacgtcac gtcctggcgt gggcgtggtg gtgaccgata
     3001 gccagcacaa cattatttcc cctgctggtg ggacactacc gctctctatt cctgatgatg
     3061 cagacagtat ccgcgaatga atgtctatcc agtcagcacg acaagcgtac caccagaaac
     3121 cgggcgattt gaagccacgg caacggtgag aataaatttt gattaacgcg tgaacgtatg
     3181 aagaaaaaaa cgatatatca gtgcgttatt ttgttcttta gccttcttaa catccatgtc
     3241 gggatggctg ggcctgaaca agtcagtatg catatttatg ggaatgtggt cgatcagggc
     3301 tgtgatgtcg ccaccaaaag tgcattacaa aatattcata ttggtgattt taatatcagt
     3361 gattttcagg ccgcgaatac cgtaagcact gctgctgatt tgaatattga tatcaccggt
     3421 tgtgccgctg gtattactgg cgcggacgtc ctttttagcg gcgaggctga cacccttgcg
     3481 ccgacactgc tcaaactaac tgacacaggc ggaagcggtg gtatggcaac ggggattgcc
     3541 gtgcattctt gatgcgcaaa gtcagcaaga aatcccgctc aatcaggtcc agcctcttac
     3601 gcccttaaaa gccggggata acacactcaa atatcaactt cgttataagt ccacaaaggc
     3661 gggagcaacg ggcggtaatg cgacggcggt tctctatttt gatctggttt accagtgaag
     3721 gagtgatgtt gaaacggata atctggattc tgttcttact gggattaacg gggggctgtg
     3781 agctatttgc ccatgatggc acggtcaaca ttagcggatc gtttcgccgt aatacatgcg
     3841 tgctggcaca ggatagcaag caaatcaatg tgcagttagg cgatgtctcg ctgacacgtt
     3901 tttttcatgg gaattatggc ccggagaaat ctttcatcat taatttgcag gattgcggaa
     3961 cagatgtcag cacggttgat gtcacctttt caggaacccc cgacggagtg cagagcgaaa
     4021 tgctgtccat tgaaagtggt acggatgcag ccagtgggct ggcaattgcg attctggatg
     4081 atgcgaagat attgattccg cttaaccagg cgagtaaaga ctacagccta catagcggta
     4141 aggtgccatt aactttttat gcgcaattgc gacctgtcaa tagcgatgtg cagtcgggta
     4201 aagtgaatgc cagcgcgaca tttgtacttc attatgacta atacgtggaa cagattagcg
     4261 ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg aattgcaggc aggagtggtg
     4321 gtcggcggaa cgcgatttat ctttccggca gacagagaat cgatatctat tttactgact
     4381 aataccagtc aggaatcttg gcttattaac agcaaaatca atcgcccaac gcgttgggcc
     4441 gggggggaag cgtcgacagt gccagcacta ttactggccg ctccgccact tattctcctg
     4501 aagcccggta ctacaggcac gttgcgcttg ctgagaacgg aaagcgacat cttgcctgtg
     4561 gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc catccggcaa agttgaaaat
     4621 cagagcgtaa aagtggcgat gcgctcggta tttaaactgt tctggcgacc cgaaggtttg
     4681 ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga cacggaattc gcagggtgta
     4741 caactcacta acccaacgcc ttactacatt aacctgattc aggtgagcgt aaatggtaaa
     4801 gcgttaagta atgcgggagt cgtgccgcct aaaagccagc gtcagacaaa ctggtgtcag
     4861 gtaatagcac cgtgtcatgt cgcctggcgg acgataaatg attacggcgg gttgagtgca
     4921 aagaaggagc aaaatctgcc ctgaaacagg ttctgaaaac gtttgcgttt tttttgccgc
     4981 aggtcaattc ccttttggtc cgaactcgca cataatacgc ccccggtttg cacaccggga
     5041 atccaggaga gttcatgtac taccccttcg ttcgtaaagc ccttttccag ctcgatccag
     5101 agcgcgctca tgagtttact tttcagcaat tacgccgcat taccggaaca ccgtttgaag
     5161 cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg catgggcctg acgtttaaaa
     5221 atccgcttgg tctggcagcc ggtcttgata aagacgggga gtgcattgat gcgttaggcg
     5281 cgatgggatt tggatcgatc gagatcggta ccgtcacgcc acgtccacag cctggtaatg
     5341 acaagccgcg tctctttcgt ctggtagatg ccgaaggttt gatcaaccgt atgggcttta
     5401 ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa ggcccattat gacggcgtct
     5461 tgggtattaa tatcggcaaa aataaagata cgccagtgga gcagggcaaa gatgactatc
     5521 tgatttgtat ggaaaaaatc tatgcctatg cgggatatat cgccatcaat atttcatcgc
     5581 cgaatacccc aggattacgc acgctgcaat atggtgaagc gctggatgat ctcttaaccg
     5641 cgattaaaaa taagcaaaat gatttgcaag cgatgcacca taaatatgtg ccgatcgcag
     5701 tgaagatcgc gccggatctt tctgaagacg aattgatcca ggttgccgat agtttagttc
     5761 gccataatat tgatggcgtt attgcaacca ataccactct cgatcgttct ctggttcagg
     5821 gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg tccgcttcag ttaaaaagca
     5881 cagaaattat tcgccgcttg tcactagaat taaacggtcg cttaccgatc atcggtgttg
     5941 gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc tgcgggtgcc tcactggtgc
     6001 aaatttattc tggttttatt tttaaaggtc cgccgctgat taaagaaatc gttacccata
     6061 tctaattatt tcttcgaatt cctctttata agataatcag ggctttattt tcagacctgg
     6121 ttgttttata ttcatcgttg ttgcttattt agacattttg tacttttatt gttgaggtta
     6181 ttaagcgaag cgacaatgga ttgtgttgct gcggttttat agggtagggg agaggcagat
     6241 gcgaattaaa ccagacgata actggcgttg gtattacgat gaagagcacg atcgaatgat
     6301 gctcgattta gccaatggta tgctatttcg ctcacgtttt gcgcgcaaaa tgttgactcc
     6361 agatgccttt tctcccgcag gcttttgtgt tgatgatgcc gcgctctatt tctcttttga
     6421 agaaaagtgc cgcgatttta atttatctaa agaccaaaaa gccgaactgg tgctgaatgc
     6481 actggtagca attcgttatc tcaaaccgca aatgccgaaa agctggcatt ttgtctccca
     6541 tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc gtttggttga gcgatactca
     6601 cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat gccgcgctat gcctgctggc
     6661 acaaccctgc gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca ttaaaatcat
     6721 gaacgacagg ctgaaaccgc aggtgaatgt tgacagcttc agcctcgaac aggcagtcta
     6781 acgcgtcagc ttaagggcag tcttcggtac acagctacag caaagaatgg tgccatcatc
     6841 gcccattgct gattttttca gcggtgtgac ttcgccttct aaaagctgaa cacggcaact
     6901 tccacaaatg cccgcgcggc aagaataagg gatacgaatt ccctgatttt ctaattgctc
     6961 cagcaacact tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa tatctacatt
     7021 tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg gcagctgcgc cgtaaatttt
     7081 tgccggagcc gttgccagaa tttccacctc atcgccaacg cgaatcacgc cgctattacg
     7141 ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc tgggcagtgc ggaaagattg
     7201 caatgttttt aatggttcgc ctgccggatg tttttgccct ttttctgggc tgacggtggt
     7261 gaaaatacag cggctacaag gtttaaccac atcaaacacc acatcaccaa tgcgaatcac
     7321 tttccagcta tcttcttccc aggctgacgc gccggaaacc accagattgg ggcggaattg
     7381 ctccattttt acactggccg gacaacgttg ttggagatca cgtaacgagg cttcgttagc
     7441 aagaaggtaa ggatagccat cagcaaatga cagaggtaca gtgttgtggc gtttcacgcg
     7501 ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg cgggagaaaa atccacttag
     7561 ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa tgtgtgcccc aaacttcggt
     7621 tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat gcgctactgc catctggtgc
     7681 ggtgagatgc aagccatcat gcacaggtga aggagtaaac cgtaccatct ggggaaactg
     7741 gcgagcggta ataaacgtac cgtcaggttc cgtgatcata aagatgcgat cgaaggccag
     7801 accactgaca tcagccagag catgtgtaag accaatgccg cgcatcgatt taacaggatg
     7861 aataaaaagc cgggttaatg tcgccacggt gcggtcctca aatgaaaata agccctcaac
     7921 tttatgacat gacgcgctta ttagctataa tgcgcaacaa ttttcttagc gcctgtcccg
     7981 ttaggctatt ttatttgtca gtttggccct gggttgtgct cgaaatcctc acgtacttgt
     8041 gtacgctccg gtttctccgc gctctccatg tccagactga ctgcaacaat tacgcctact
     8101 gcggtaggct cttagagtaa aagtgacgat atgaattctc tgtttgccag tacggcccgt
     8161 gggctggaag agctgttaaa aactgaactg gaaaatctgg gggccgttga atgccaggtg
     8221 gttcagggtg gggtccattt caagggcgac acacggcttg tttaccagag cctgatgtgg
     8281 agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta cagcgattta
     8341 gacctctatc tcggtgttca ggcgatcaac tggacagaga tgtttaatcc tggcgcgacc
     8401 ttcgctgtcc acttcagtgg tttgaatgac accatccgca acagtcagta cggcgcgatg
     8461 aaagtgaaag acgcgatcgt cgatgctttc acacggaaaa atctgccgcg tccaaatgtt
     8521 gatcgcgatg cgccggatat ccgcgttaac gtctggctgc ataaagaaac cgccagtatc
     8581 gctcttgatc tgagtggtga tggtttacat ctgcgtggct atcgcgatcg tgctggtatt
     8641 gcgccgatca aagaaaccct ggcagccgct attgtgatgc gatccggctg gcagtcagga
     8701 acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc tgattgaagc ggcgatgctg
     8761 gcgaccgatc gcgcaccagg cttgcaccgt gggcgttggg gctttagcgg ctgggcgcag
     8821 catgatgaag ctatctggca ggaagtgaaa gcggaagcgc aaactcgcgc ccgtaaaggc
     8881 ctagctgagt atagctctca tttttacggt tcggacagcg acgcacgggt gattcaacgt
     8941 gcgcgcacta acgcccgtct tgcggggatt ggtgaactga tcacctttga ggtgaaagat
     9001 gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg gtacagtgtt gagcaacccg
     9061 ccatacggtg aacgtctgga cagcgaaccg gcgctgattg cgctgcatag tctgctgggc
     9121 cgaatcatga aaaaccagtt cggtggctgg aatctctctt tgtttagtgc ctcgccggat
     9181 ctgctaagct gcctgcagct gcgtgcagac aaacagtaca aggcgaaaaa cggcccgctg
     9241 gactgcgtac agaaaaatta ccacgttgcc gaaagcacac cagacagtaa accggcaatg
     9301 gtagcggaag attacgccaa ccgcctgcgt aagaacctca aaaaattcga gaagtgggca
     9361 cgccaggaag ggattgaatg ttaccgcctg tatgacgccg atctgccgga atataacgtt
     9421 gccgttgacc gttatgccga ctgggtggtg gtgcaggagt atgcgccgcc aaaaactatt
     9481 gatgcgcaca aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat ttcggtactg
     9541 gggattgcgc caaacaaact ggtgctgaaa acccgtgaac gccataaggg caaaaaccag
     9601 taccagaaac tgggcgagaa gggcgagttt cttgaagtta ccgaatataa cgctcacttg
     9661 tgggtgaacc tgacggatta tctcgatact ggtctgttcc tcgatcaccg tatcgcccgc
     9721 cgtatgctcg gtcagatgag caaaggcaaa gatttcctca acctgttttc ttataccggc
     9781 agcgccaccg tgcatgcggg attaggcggt gcacgcagca ccaccaccgt ggatatgtcg
     9841 cgtacttatc tggaatgggc agaacgcaac ctgcgtctga atggcctgac taggcgtgcg
     9901 catcgcctga ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga acagttcgat
     9961 ctgatcttta tcgatccgcc aaccttctct aactcaaaac gaatggaaga tgcgtttgat
    10021 gttcagcgcg atcatctggc gctgatgaaa gatttgaaac gtctgctgcg tgcaggtggg
    10081 acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg atctcgacgg cctggcaaaa
    10141 ctgggactga aagcacaaga aattacgcaa aaaacgctct cccaggattt cgcccgtaac
    10201 cgccagatcc acaactgctg gctgattacc gcagcctgaa aggaatagta atgtcattaa
    10261 tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat aacgcagaac
    10321 tgcatatcga agataacgaa cgtgtttgtc tggtgggccg caacggcgca ggaaaatcga
    10381 cgttaatgaa aatcctcaac cgtgaacaag ggctggatga cggtcgcatt atttatgagc
    10441 aagatttgat tgtagctcgt ctgcaacagg atccgccgcg taacgttgag ggtagcgttt
    10501 atgatttcgt tgccgaaggc attgaagaac aagcggaata tctgaaacgc tatcacgata
    10561 tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct caacgaactg gcgaaggttc
    10621 aggaacagct ggatcaccac aacctgtggc agctggaaaa ccgcatcaac gaagtgctgg
    10681 cgcaactggg gttagatcct aacgttgcgc tgtcgtcgct ttccggcggc tggttgcgta
    10741 aagcggcatt aggacgcgcg ctggtgagca atccgcgcgt attgctgctg gacgaaccga
    10801 ctaaccatct ggatatcgaa accatcgact ggctggaggg gttccttaaa accttcaatg
    10861 ggacgattat tttcatctcc cacgaccgtt cgtttatccg caatatggcg acgcgcattg
    10921 ttgatctcga tcgcggcaag ctggtgacct atccagggaa ttacgaccag tacctgcagg
    10981 aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc cgagttcgat ctcaaactgg
    11041 cgcaggaaga agtgtggatt cgccagggga tcaaagcacg ccgtacccgt aatgaaggcc
    11101 gcgtacgcgc cctgaaagcg atgcgtcgcg aacgtggtga acgtcgcgaa gtgatgggta
    11161 ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa gatcgttttc gaaatggaag
    11221 acgtttgcta ccaggttaac ggtaagcaac tggtgaaaga tttttctgcc caggttctac
    11281 gtggcgacaa aattgccctg attggtccga atgggtgcgg caaaaccacg ctgctgaaac
    11341 tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca cgttggcacc aaactggaag
    11401 tggcttattt cgatcagcac cgcgcggaac tggatcccga taaaacggtg atggataacc
    11461 ttgccgaagg taagcaagag gtgatggtta atggcaagcc acgccacgta ttgggctatt
    11521 tgcaggactt tctgttccat ccgaaacggg cgatgacgcc agtacgtgcg ttttctggcg
    11581 gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac ttattgattc
    11641 ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga actgctggaa gaactgatcg
    11701 acagctatca gggcacggta ttgctggtta gccacgatcg tcagtttgtc gataacaccg
    11761 ttacagaatg ctggatcttc gaaggcggcg gtaaaattgg tcgttatgtt ggcggttatc
    11821 atgatgcccg tggtcagcaa gaacaatatg tggcactcaa acagcctgcg gtgaaaaaaa
    11881 gcgaagaagc cgccgcgcca aaagcagaaa ctgtaaaacg cagcagtagc aaactaagct
    11941 ataaattgca gcgcgaactg gagcagctac cgcaattgct cgaagatctg gaggcgaagc
    12001 tggaagccct acagacgcaa gtggcggatg cttccttctt cagtcagccg catgagcaga
    12061 cgcaaaaagt gcttgctgat atggctgctg cagagcagga gctggagcaa gcctttgaac
    12121 gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt cgatttaaac
    12181 agagacagct gacaacggta aaattgcctg atgaactggg tttatcaggc atgaaaacca
    12241 acactttgtt agtcatctgt aaaatagcgc atcattaagg agtaccaatg tgcgaacatc
    12301 atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg ttaccgcgcc
    12361 ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc gtggcgtggg
    12421 atgcccctcg gcagcgtccc accgcctatg cgttggctgc actgttcatg ctgttgctgt
    12481 ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt taccagtgaa attacattac
    12541 tggaaattcc cggcgtgctt ttttctgagg actacgccag cctcggcacc tttttcctgt
    12601 tgtttgtgca actggttccc gcgttttgtc tgataaccat tctgttactg gtgaatcgcg
    12661 cggaattacc ggtccgttta aaagagcaac tggcacgggt gctttttcaa ctcaaaacct
    12721 ggggaatggc ggagattttc ctcgcgggtg tgctggtcag tttcgttaaa ctgatggctt
    12781 acggcagcat tggggtaggc agcagctttc tcccctggtg tttattttgt gtcctgcaac
    12841 tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc ccgatgccag
    12901 aactgcgcca gccgctaaaa ccaggcgtca cggggatacg tcaggggctg cgttcgtgct
    12961 cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt agtaccaaag
    13021 ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc gctgcttgta acgtccatca
    13081 tgctgtatct tccggctaat attttgccca tcatggtgac ggatttatta ggctcgaaga
    13141 tgccgtcgac gattctcgct ggggtcattc tgttatggag cgaaggctct tatcccgtcg
    13201 ctgcggtgat ctttctggcc agtattatgg tgccaacgtt aaagatgatc gccatcgcgt
    13261 ggctgtgttg ggatgccaaa gggcatggca agcgcgacag tgaaagaatg catttgattt
    13321 atgaagttgt tgagtttgta ggccgctggt cgatgattga cgttttcgtt atcgcggtgc
    13381 tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg ggtgcattaa
    13441 tgtttgcttt agtcgtcata atgacaatgt tttctgctat gacgtttgac ccgcgtttgt
    13501 cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg acagtatgga atctaataat
    13561 ggggaagcca aaatccagaa agtgaagaac tggtctcccg tgtggatatt tcctatcgtc
    13621 acggcgctca ttggggcctg ggttcttttt tatcattaca gccatcaggg accggaagtg
    13681 accctgatca ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat taaaagccgt
    13741 agcgttgacg tcggcgtggt tgaaagcgcc acactggctg atgatttgac gcacgttgaa
    13801 atcaaagcgc ggctgaattc cggtatggaa aaattgctgc ataaagacac cgtcttttgg
    13861 gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc tgggaacgct gctgtctgga
    13921 gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata cgatttgctg
    13981 gactcaccac cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct cgatagcaaa
    14041 aaagccgggc agctctcgcc aggagatccg gtgctgttcc gtggctatcg ggtaggttcg
    14101 gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca gttatcaact gttcatcaat
    14161 gcaccttatg accgactggt gaccagcaat gttcgcttct ggaaagatag tggcattgcg
    14221 gttgatctga cgtcagcggg gatgcgtgtg gagatgggct cattgacaac gctgcttagt
    14281 ggcggtgtta gctttgatgt gccggaaggt ctggatttag ggcagccagt ggcaccgaaa
    14341 acagctttcg ttttgtatga tgatcagaag agcattcagg attcgttgta caccgatcac
    14401 attgattatc tgatgttctt taaagattcg gtacgcggtc tgcaaccggg agctccggta
    14461 gaattccggg gtattcgcct gggtaccgta agcaaagtgc cattctttgc gccgaatatg
    14521 cgtcagacat ttaacgatga ttaccgtatt ccggtactga ttcgtatcga gccagagcgg
    14581 ctgaaaatgc agcttggcga aaatgcggat gttgttgagc accttggcga attgttgaaa
    14641 cgtggtttac gcggatcgct gaaaaccgga aacctggtca ctggtgcact gtatgttgat
    14701 ctcgatttct atccaaatac gcctgcaata accggtattc gtgaatttaa tggttatcag
    14761 attatcccga ctgttagcgg cggcttggcg caaatccagc aacgactgat ggaagcgttg
    14821 gataagatca acaaactgcc attgaatccg atgattgaac aggcaaccag tacgctttct
    14881 gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa caagatcctc
    14941 gccagccagt cgatgcagca gttgccgacg gatatgcagt caacgttgcg tgaattgaat
    15001 cgcagcatgc agggcttcca gcctggctcc gcagcctaca acaagatggt ggcggatatg
    15061 cagcgccttg atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct caatgagaag
    15121 agtaacgcgc tggtatttga agcgaaggac aaaaaagatc cagagccgaa gagggcgaaa
    15181 caatgaaaaa gtggctagtg acgattgcag cactgtggct ggccggatgc agctccggcg
    15241 aaattaataa aaactattac cagttacctg tggtgcagag cggtacacaa agtaccgcca
    15301 gccagggcaa tcgtctgtta tgggtagagc aggtcactgt tcctgactat ctggcgggga
    15361 atggtgtggt ttatcaaacc agtgatgtga agtatgtgat tgccaacaac aacttgtggg
    15421 ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc caacctgagt acgcaactgc
    15481 ccggctgggt ggttgcctcc cagcctctgg gaagcgccca ggacacgctc aatgttaccg
    15541 taacggagtt taacggtcgc tatgatggca aggtcattgt cagtggtgag tggctgttga
    15601 accaccaggg acaactgatc aaacgtccgt tccgtctgga aggagtgcaa actcaggatg
    15661 gttacgatga gatggttaaa gtgctggccg gtgtctggag tcaggaagcc gcttctattg
    15721 cacaagagat aaagcgtcta ccttaattat aaagatttgt aaatataacc gtctccggta
    15781 tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa aatttgttcc tcttcacatt
    15841 ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga cagtggcgtg aattttgcgc
    15901 attgacggca gttatgattc gcggtattgc ttaactgtga ttgcacattt agtaatcact
    15961 gttttctttt ccaccagaaa ccagtatgag ggaaacgagg catgaagaga caaaaacgag
    16021 atcgcctgga acgggcacat caacgtggtt atcaggccgg catcgccgga cgctcaaaag
    16081 aaatgtgtcc ctatcagacg ctgagtcaaa ggtcacaatg gctgggaggc tggcgagaag
    16141 ccatggcgga cagggtagta atggcctgat tctgtctctt taaaaagaaa cctccgcatt
    16201 gcggaggttt cgccttttga tactctgtct gatgataatc agaaggcaga cgtatcctgg
    16261 aacagaccga ctttcaggtc gctggcggta tagatcagac gacaatcaac cagcacttcg
    16321 ccatctgcca gacccataat cagacgacga ttaacaatgc gtttaaagtg aatacggtag
    16381 gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc aacgcccagc
    16441 gcgcggcctt taccttcgcc gcccagccag ccgaggtaga accctaccag ctgccacatt
    16501 gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa tgaagtggca tccgaagaac
    16561 cacagatccg gattgatatc cagttctgct tcaacatacc ctttgtcgaa gttaccaccc
    16621 gtttcggtca ttttgaccac acggtctatc atcagcatgt tcggtgctgg caattgcggg
    16681 cctttagcgc caaacagttc accgtgacca gaggcaagaa ggtcttcttt tgtataggat
    16741 tcgcgtttat ctaccatgtt ctctgtaagc cttattttat tgaagcacgc aggatagcta
    16801 acacgtgtac gctgaacaag tccgatcagt tcggaataaa ccagttcagc caacgtaatg
    16861 gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt tcctggatgg
    16921 tttgcatcag cgtcgtttgg ccttcgccat cccacaccag atttaataat aacggcagtg
    16981 cgtcagtcac atcgtctact gcccagatgg tgaatttgcc ttcttctacc gctttcacca
    17041 gttcactgtg aagacttaaa tggcgaacgt tagcagtggg gataatgaca ccttgtttcc
    17101 ccgttaactc acgttgctgg caaatagcaa agaagccttc gattttctca tttaaaccac
    17161 cgaccggctg gacgcggccg aactgatcga ctgaacctgt gatagcgata ctctgattca
    17221 ctggcacatc agcgagggcg cttatcaggg cgcagagttc agccatcgag gcactatcgc
    17281 catccacttc actgtatgac tgctcaaatg tcagcgatgc tgagaagggg atctgttgct
    17341 caagctgtag ttccgacatc aggaacgcct gcatgatcat catccctttc gcatggatat
    17401 tgccgccaag ctccgctttg cgttcgatgt cggtgaattc accatcgcca atatgcacaa
    17461 cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat tcaatgaccg
    17521 aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc aatcaggatt tgctcctgaa
    17581 ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc tgctgcagca
    17641 ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg acctctttac
    17701 actggcggag gatccactgc gggctaagcg gaagtgtttc ttgttcaccg gtgtagcgtg
    17761 ctgcttcgcg gataagtacc ggccaagcat ccgctcccgg ggcaggtaag tgattatgtc
    17821 tggcggtaaa tgtcacccag cgacaccact ggcttactga ctccgcatcg acaatctgca
    17881 gagtatcttc aaattcgcta taaatagcct gctctgaaag ctctggctcc atctcctgga
    17941 aatcagccaa tgattcgcgt tcgcctacca gaatgacttt cagcttcaat ggcattgaag
    18001 gcacagagac ggggagaggg cgcgactcat caaacgcaac ccagtcaaaa cgctcgcggt
    18061 taacgatatt tttcagccgc atccacagca gaggttgcgc cagcagtgta cgcaaagaga
    18121 tgatgagaat accgccattt gcctgatgca ccagaccagg ctgcagggta atgtcgccat
    18181 taaactggcg caggcagcca aagagttgct ccgcttctac ccagtcggca gcgacaactt
    18241 gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg atggagtggc
    18301 cggaaacctc atagtgatcg cccaccagtt ggcctgcatc gctttgtagc gtacgcgcgg
    18361 catcggcaat aagattcaga tactcagact cttccggggc cttagccagc ataaaagagg
    18421 aggatgctcg cgtatgcagc aactgctcca gcgcaaattg cagtcgcggt tgagtatcac
    18481 tgaataaagg atcgttttcg tcaatcaaat gtggctgagc aaatatttcc tgatagctat
    18541 cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt gatgtttttt
    18601 agtctgttgt caaagccgcg attataccgt aaccggcact acagcacacg tagaaagcac
    18661 cgacaatact cctggctggg cgttaaagct cacaggatgg agattctttt cttcactggc
    18721 ctaaaaagct gatattctgt aaagagttac acggtaacat tgagatcgct atgaaatatc
    18781 aacaacttga aaatcttgaa agcggttgga aatggaagta tctggtgaag aagcatcgcg
    18841 aaggggagtt aattacccgt tacatagaag ccagtgccgc ccaggaagcc gtcgatgtgt
    18901 tgctctcgct cgaaaatgaa cccgtgctgg taaatggctg gattgacaag catatgaatc
    18961 cggaactggt caatcgcatg aagcagacca ttcgggccag acggaagcgc cattttaatg
    19021 cggaacacca gcatacgcgc aaaaaatcga tcgatctgga atttattgtc tggcaacgtc
    19081 tggctggtct tgcacagcgt cgcggtaaaa cgctttctga aacgattgtt cagctgattg
    19141 aagatgcgga aaacaaagag aaatacgcga ataaaatgtc ttctcttaag caggatctgc
    19201 aggcattgct gggtaaggaa taactgacga aagtcagttc aatttactaa aggcaaaaaa
    19261 aaccccgcag cagcggggtt tttctaccag acgataactt aagcctgcgg ctgagttaca
    19321 acgtctttga tacctttaac ttcgatctct acgcgacgat ccggagccaa gcagtcgatc
    19381 agtgcagcac gctgtttcac gttgtcacag gtgttgccag taaccgggtt ggattcgccc
    19441 ataccacgtg cggagatctt gtctgccggg atacctttgg agatcaggta atcaacaaca
    19501 gactgagcac ggcgctcgga cagaccctgg ttgtaagcgt cagaaccgat gcggtcagtg
    19561 taacccagaa caactacgga accgtctttc ggatctaggt tgctcagctg gctgtacagc
    19621 tgatccagag cagcctgacc ttccggtttc agggttgctt tgttgaagtt gaacagaacg
    19681 tcagacttca gagtgaagtg cttggtctgt acttccggtg ccggagctgg agcaactacc
    19741 ggagctgctt cgccctgacc gaaacggtag gaaacaccca ggctcagcag accgttgtcc
    19801 ggacgagtac cgatggtgtt tgcgtcaccg atgttgttgg tccactggta ttccagacgg
    19861 gtagcgattt caggagtgat cgcgtactca acaccacctg cgaaaaccgg agaaacgcca
    19921 gtgtcgtggt ctttaaagga tgcgccacca ggtacgttag ccttggtgtc tgcacgccat
    19981 accataccac ccagacgagt gtagatgtcc agatcgtcag tgattgggta acccagttta
    20041 gcggtcagct gaacgccctg agctttgtat gcgccgttga tgttgtcgcc tttgtacggc
    20101 atacgaccta accagtcgta acccatttca aaaccaacat acgggttaac ctggtaacca
    20161 ccaaaagcac ctgcacccag ttggttttcg tgggtcggac cattgttagg aataaaacca
    20221 gtgtcatggt actgggacca gcccagttta gcaccagtgt accaggtgtt atctttcgga
    20281 gcggcctgcg ctacggtagc gaaaccagcc agtgccactg caatcgcgat agctgtcttt
    20341 ttcatttttt gcgcctcgtt atcatccaaa atacgccatg aatatctcca acgagataac
    20401 acggttaaat ccttcaccgg gggatctgct caatattaac tctaccgata tcttcggctt
    20461 atgccgagca cccctggcga tgtaaagtct acaacgtagt tgaaaactta caagtgtgaa
    20521 ctccgtcagg catatgaaaa aaaagtcttg tataaggtat gtttaatctt tttgtcagcg
    20581 acaatttaca gaaaaaaaac gcggaaaccg cttcagacaa gccttcgcaa ggaaaattag
    20641 tcacgactga aagcattggc tgggcgacaa aaaaagttct aggattaatc ctaaatttac
    20701 ttaatgatac aaattagagt gaatttttag cccggaaagt tgtctcgttg tgtgagagga
    20761 tgcgcttacc ggacgcataa taaaccccat agcgttacct tcatttgccg catcaacaag
    20821 ttcagcatgc tcttcttcag tcaaatcatc tgtcaaccaa ccgatcacca cactgtaatt
    20881 gcccgtgcgt aaagcgcgaa ccattgactc cactgtgtgg caaggggaga gctggctaat
    20941 ctgcattact ttcgttaagg gtagcccaga tgcctgaacc cattcccgac tcagtttttg
    21001 ttgcggtgtt aaccagagtt gccagcgcga ttgctgaccg agttgctgta acaatggcaa
    21061 cagtagaagt tgcgtcatca tgggctgatc ttcgcgatag acaacttcac tgataagccc
    21121 ggctgtagtg ttttccgtag agacacgcgc aattttactt gctgcggatg agaacgacga
    21181 agaacgatgt gcatagcctg aagtgtacat aatcaatcca gcccctgtga gttactgtat
    21241 ggatgtgcag tacatccagt gacaacaaag atcaacccta ttttcggaaa gagcctcgca
    21301 aattttgtcg ttggtgacgg gaaaacataa attaatcttg ccccttaaga ataagttgcc
    21361 tattttcgta gttaacggat ccgttaatgt gaatcattct tttatgttat gattttaaaa
    21421 ggaattttat gaaaagcctc tcctataagc ggatctataa atcacaagaa tacctggcaa
    21481 cgttgggcac aattgaatac cgatcattgt ttggcagtta cagcctgacc gttgacgaca
    21541 cggtgtttgc gatggtttct gatggtgagt tgtatcttcg ggcttgtgag caaagtgcac
    21601 agtactgtgt aaaacatccg cctgtctggc tgacatataa aaagtgtggc cgatccgtta
    21661 ccctcaatta ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag ctggtgcgtc
    21721 tgtcgaaata ttctctcgat gcagcgctga aagagaaaag cacgcgcaat acccgggaaa
    21781 gactgaaaga tttgcccaat atgtcttttc atctggaagc gattctcggg gaggtgggta
    21841 gactggcccc ctgaatctcc agacaaccag tatcacttaa
//
LOCUS       DAJTYH010000066        21374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTYH010000066 DAJTYH010000000
VERSION     DAJTYH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..127
                     /locus_tag="OO609_002510"
     CDS             <1..127
                     /locus_tag="OO609_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6633888.1"
                     /translation="GAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFTAHRIKD"
     gene            complement(166..513)
                     /gene="grxD"
                     /locus_tag="OO609_002511"
     CDS             complement(166..513)
                     /gene="grxD"
                     /locus_tag="OO609_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6633889.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            848..1675
                     /gene="mepH"
                     /locus_tag="OO609_002512"
     CDS             848..1675
                     /gene="mepH"
                     /locus_tag="OO609_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6633890.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1803..2384
                     /gene="sodB"
                     /locus_tag="OO609_002513"
     CDS             1803..2384
                     /gene="sodB"
                     /locus_tag="OO609_002513"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6633891.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2530..3699)
                     /locus_tag="OO609_002514"
     CDS             complement(2530..3699)
                     /locus_tag="OO609_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633892.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3865..3954)
                     /gene="ynhF"
                     /locus_tag="OO609_002515"
     CDS             complement(3865..3954)
                     /gene="ynhF"
                     /locus_tag="OO609_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6633893.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4253..5278
                     /gene="purR"
                     /locus_tag="OO609_002516"
     CDS             4253..5278
                     /gene="purR"
                     /locus_tag="OO609_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6633894.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5275..6207)
                     /locus_tag="OO609_002517"
     CDS             complement(5275..6207)
                     /locus_tag="OO609_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6633895.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            6320..7531
                     /locus_tag="OO609_002518"
     CDS             6320..7531
                     /locus_tag="OO609_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6633896.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7822..8970
                     /gene="cfa"
                     /locus_tag="OO609_002519"
     CDS             7822..8970
                     /gene="cfa"
                     /locus_tag="OO609_002519"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6633897.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9010..9651)
                     /gene="ribE"
                     /locus_tag="OO609_002520"
     CDS             complement(9010..9651)
                     /gene="ribE"
                     /locus_tag="OO609_002520"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6633898.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9866..11239
                     /gene="mdtK"
                     /locus_tag="OO609_002521"
     CDS             9866..11239
                     /gene="mdtK"
                     /locus_tag="OO609_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6633899.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11280..12536)
                     /locus_tag="OO609_002522"
     CDS             complement(11280..12536)
                     /locus_tag="OO609_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6633900.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12844..12920
                     /locus_tag="OO609_002523"
     tRNA            12844..12920
                     /locus_tag="OO609_002523"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12878..12880,aa:Val,seq:gac)
     gene            12925..13001
                     /locus_tag="OO609_002524"
     tRNA            12925..13001
                     /locus_tag="OO609_002524"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12959..12961,aa:Val,seq:gac)
     gene            13109..13414
                     /locus_tag="OO609_002525"
     CDS             13109..13414
                     /locus_tag="OO609_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6633901.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13540..14962
                     /locus_tag="OO609_002526"
                     /pseudo
     CDS             13540..14962
                     /locus_tag="OO609_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(14974..15786)
                     /locus_tag="OO609_002527"
     CDS             complement(14974..15786)
                     /locus_tag="OO609_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633902.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15790..16575)
                     /locus_tag="OO609_002528"
     CDS             complement(15790..16575)
                     /locus_tag="OO609_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6633903.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16572..17240)
                     /locus_tag="OO609_002529"
     CDS             complement(16572..17240)
                     /locus_tag="OO609_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6633904.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17304..17942)
                     /locus_tag="OO609_002530"
     CDS             complement(17304..17942)
                     /locus_tag="OO609_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6633905.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17955..20034)
                     /locus_tag="OO609_002531"
                     /pseudo
     CDS             complement(17955..20034)
                     /locus_tag="OO609_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20055..20681)
                     /locus_tag="OO609_002532"
     CDS             complement(20055..20681)
                     /locus_tag="OO609_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6633906.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21137..21346)
                     /gene="fumD"
                     /locus_tag="OO609_002533"
     CDS             complement(21137..21346)
                     /gene="fumD"
                     /locus_tag="OO609_002533"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6633907.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5336 a         5552 c         4965 g         5521 t
ORIGIN      
        1 aggtgcgcca atatccgtca tccgccagta cattgaacaa cagcaaacac ctggtcaggt
       61 ggaaaaccgc gccttatatc cccgccctga aggacgaggt tttacggcgc accggataaa
      121 ggactgacgg atgcccgttc gcatccgtca gtattgcagg acggattatt ccgcgtccgg
      181 ctcttcagac ttgtatttag cggcagtttc tttgatcagc tgctgcagtt cgccacgctg
      241 atacatttcg atcacgatat cacaaccgcc gaccagctcg ccgtcaaccc acagttgcgg
      301 gaaggtcggc cagttagcat atttcggcag ttccgcacga atgtccggat tctgcagaat
      361 atcaacatag gcaaaacgtt cgccacatgc ggcaagcgcc tggactgcct gggcagagaa
      421 accgcagctc ggcagtttcg gtgaaccttt catgtacagc aggatcgggt tttcagcaat
      481 ctggcgttgg attttttcga tagtggtgct cattgtcttg cttcctcaaa cttctttacg
      541 gcagtaatct gacattgtag cgggtcagtg cggcatcgga aaataacatt ttcatcacgc
      601 ttttgctatt ttatcccttt gctctatttg ttgcatttca aatattcgtt tcttctctga
      661 tgcgcggcaa actggcgtat ttataaccat ttttagcttt cactgctgca tttttttgcg
      721 ctcgccaacg aaacgtattt tttaacaata aaagctatta actttctctt cttctatgca
      781 ttagaatcat caagttttgt aaatcagacg caggcatgat agacctgcct taacagaggg
      841 acgctcagtg gcgcggataa accgtatttc gatcacgctc tgtgctttac tttttaccac
      901 cctgccttta acgcctatgg cccatgcttc aaagcaagcc agggagagtt ctgctaccac
      961 tcatatcacc aaaaaagcag ataaaaagaa aagcacggca accaccaaaa agacccagaa
     1021 agccagccag aaaacagcga aaaaagccgc cagtaaaagt atgaccaaaa gcaaaaccgc
     1081 ttcttctgtt aaaaaatctt ccattaccgc ttctaaaaac gccaaaactc gcagcaaaca
     1141 cgccgtcaat aaaacggcct cagccagctt taccgaaaag tgtaccaaac gtaagggcta
     1201 taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga acccttgccg acgcgcacaa
     1261 agcgaaggtg caaaaagcca cgaaagtggc aatgaataaa ctgatgcagc aaattggtaa
     1321 gccatatcgt tggggcggca gctcaccgcg taccggtttt gattgcagcg gcctggttta
     1381 ttacgcttat aaagatttgg tgaaaattcg tattccgcgt acggcgaatg aaatgtatca
     1441 cctgcgtgat gcaggaccta tcgaacgtag tgagctgaaa aacggcgacc tggtcttttt
     1501 ccgtactcag ggacgcggta cagccgatca tgtcggcgtg tatgtcggca acggcaaatt
     1561 tattcagtca ccgcgcacag gtcaggaaat tcaaatcact tctctcagtg aagactactg
     1621 gcagcgccac tatgttggcg ctcgtcgggt aatgacccca aaaacacttc gctaaaactt
     1681 taccctgttg ttacggcaac agggtaagtt catcttttgt ctcacctttt aatttgctac
     1741 cctatccata cgcacaataa ggctattgta cgtatgcaaa ttaataataa aggagagtag
     1801 caatgtcatt cgaattacct gcactaccat atgctaaaga tgctctggca ccgcacattt
     1861 ctgcggaaac tatcgagtat cactacggca agcatcatca gacttatgtc actaacctga
     1921 acaacctgat taaaggtacc gcgtttgaag gtaaatcact ggaagagatt attcgcagct
     1981 ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa ccatactttc tactggaact
     2041 gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt cgctgaagct atcgccgcat
     2101 cttttggcag ctttgccgat ttcaaagcgc agtttactga tgcagcgatc aaaaactttg
     2161 gttctggctg gacctggctg gtgaaaaaca gcgatggcaa actggctatc gtttcaacct
     2221 ctaacgcggg tactccgctg accaccgatg cgactccgct gctgaccgtt gatgtctggg
     2281 aacacgctta ttacatcgac tatcgcaatg cacgtcctgg ctatctggag cacttctggg
     2341 cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc ataataactg atggcaaatg
     2401 cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg atgaggcgtt aacagcgcat
     2461 tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca gaaggttatc cttctgcgtt
     2521 tttgtttaat tagctgttag caacgcaaac tgtttcaggt tgttttctgg ctgacataaa
     2581 caccagcaat aatgccagtc ccgcgacaat cgcccccgtc accggcacaa agctgtatcc
     2641 cagcccagcg gaaattaccg caccaccagc agctgctccc agcgcatttc caagattaaa
     2701 ggcaccaata ttgactgatg aagacagacc tggcgcttca ctggcgacat gcatcacgcg
     2761 catctgtaac ggaggtacaa ccgcaaaggt tgctgcgccc cacaccacca tgctaatagc
     2821 tgcgccgaac tcattgcggg ccaggaacgg gattgccagc ataatcacca tcagcagcaa
     2881 caaaaagcct ttcaacgtgc cgttaactga acgatctgcc agtttgccgc cgagatagtt
     2941 accgatagag aatccgacac caatcagcac cagcattgcc gtgacgaaca ccggtgttgc
     3001 gtgggtaata ctttgcagta ccggagagat ataggtgtag agggtaaaca ttgcaccagc
     3061 tcccagtacc gtcgtcagca atgcagacag cacctgcgga cgcattaata ccgccagctc
     3121 ttttttcact tcaggtcgtg cccctgtacc acctttaggt aatgagaaga acagacttac
     3181 cattgaaatc actcccagcc ccgccgttgc cagaaatgac atccgccagc cgatggtttc
     3241 acccaaccag gtcgccgccg gcacgccacc gatatttgcc agggttaacc ccataaacat
     3301 agtggcaact gcgctggcct gtttatgttt tggcaccacg cttgcggcca cgactgaacc
     3361 caaaccaaaa aatgctccgt gattcaggct ggtcaaaata cgtgaaagca tcagggtcat
     3421 ataatccggc gcgatggcgg aaagaacgtt gcctagcgtg aaaattgcca tcaggaaaat
     3481 caacgcactg cggcgggcac gatgagaaag tagaagcgtc atcagcggcg cgcccaccat
     3541 tacgccaact gcataggcac tgattaacat tccggcagcg ggaatcgaga catccacacc
     3601 gcgcgcaatg acgggcaaca agcccattgg cgagaactcc gttgtcccga taccaaacgc
     3661 gccaatcgcc agcgccagca acggatagtt aattttcatg ccttatctcc acctcttcgc
     3721 gtcattacgc gatattcatt aaagtggcga aagcatgaca gcaatcacaa aaaaatgaaa
     3781 ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa acaggaagga gatgcgaggg
     3841 agaacgcgct ccctcgagag gaaatcagtg cagcgcggca gtcaaaccca cggctacgat
     3901 caaaccgagg acgataaccg ttgttaccag tgaaaattta aggtcggtgc tcatcaagtt
     3961 ttctcctttt ttattaccac acaaaaagtg atattacgca tttttacaca ctgtgatgaa
     4021 aaaatctccc gtcatttata atgataagtg tttttaccac ttcccctttt cgtcaagatc
     4081 ggccaaaatt ccacgcttac actatttgcg tactggccat tgaccccttc ctgacgctcc
     4141 gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg taaggtgtgt aaaggcaaac
     4201 gtttaccttg cgattttgca ggagctgaag ttagggtctg gagtgaaatg gaatggcaac
     4261 aataaaagat gtagcgaaac gagcaaacgt ttccactaca actgtgtcac acgtgatcaa
     4321 caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg tgggcagcga ttaaagaatt
     4381 acactactcc cctagcgcgg tggcgcgtag cctgaaggtt aaccacacca agtctatcgg
     4441 tttgctggcg accagcagcg aagcggccta ttttgccgag atcattgaag cagttgaaaa
     4501 aaattgcttc cagaaaggtt acaccctgat tctgggtaat gcgtggaaca atcttgagaa
     4561 acagcgggct tatctgtcga tgatggcgca aaaacgcgtc gatggtctgc tggtgatgtg
     4621 ttctgagtac ccagagccgt tgctggcgat gctggaagag tatcgccata tcccaatggt
     4681 ggtgatggac tggggtgaag caaaagctga cttcaccgat gcggtcattg ataacgcgtt
     4741 cgaaggcggc tacatggccg ggcgttatct gattgaacgc ggtcaccgcg aaatcggcgt
     4801 catccccggc ccgctggaac gtaacaccgg cgcaggccgc cttgccggtt ttatgaaggc
     4861 gatggaagaa gcgatgatca aggtgccgga aagctggatt gtgcagggtg actttgaacc
     4921 tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag ccgcatcgcc ctactgccgt
     4981 cttctgtggt ggcgatatca tggcaatggg cgcactttgt gctgctgatg aaatgggcct
     5041 gcgcgtcccg caggatgttt cgctgatcgg ttatgataac gtgcgcaacg cgcgctattt
     5101 tacgccggcg ctgaccacga tccatcagcc aaaagattcg ctgggtgaaa cagcgttcaa
     5161 catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg cagtccattg aagtgcatcc
     5221 gcgtttgatt gaacgccgct ccgtggctga cggcccgttc cgcgactatc gtcgttaatc
     5281 acccgttgcg ggagtctctt ccggctcccg cagccactcc ttattcagcg tctcactatc
     5341 gctgagatac tcaagcagcc aggttaacgc aggcgacata tcattttgct gccatgtcag
     5401 acaacatgcc gaatccggga aggggttttc cagttcaagt gctacccact tcccctcatt
     5461 aagccacggt ttggcgaaat gtgttggcac catccctatg cataatcctg ccgagataca
     5521 ggttgccgat gattcccagt caggtacgac aagtcttttt tggttatcca gcaaccaggt
     5581 aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc aacgacggcc agttgcgcaa
     5641 cgtatcatcg ctgaacgggc catccatcaa cgccagcggg tggtgactgg caacaacgca
     5701 actccagctt agcatcccca tatcccggaa ggcataacga ccgcctaccg gaatcgcgcg
     5761 tgttgcgcca attgccagtt ccacgcgccc gtcggaaagc gcatcccaga caccgttgaa
     5821 cacttcctga aagacaagaa gttcgacatc atcaaaatgg cgataaaaat caacgatcat
     5881 ctgccgtgta cgttctggcc tgacaatatt atccactgcg atagctaact gaccgcgcca
     5941 gccgttcgct atctgctgac attgctggcg ggtgatctgc atttttttga caacagagcg
     6001 cccttctttg agaaaccacg ctccagcagc ggtcagctcc acatcacggt gccgtcgttc
     6061 aaagagcggt accgccagcc actcttccag ctgacgcacg gtatagctga ccgcagaagg
     6121 aacacgatgc agctcctgtg ccgcagcgct aaaactacca ttacgcgcta ccgcatcaac
     6181 aacttcgagt gaatattctg accacatagt ctgcctgcaa aatttttgaa accagtcatc
     6241 aaatattacc gtttcacaac actaatttca ctccctacac tttgcggcgg tgtttaattg
     6301 agagatttag agaatataca tgcaacctgg gaaaagattt ttagtctggc tggcgggttt
     6361 gagcgtactc ggttttctgg caaccgatat gtatctgcct gctttcgccg ccatacaggc
     6421 cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt agtctgttcc ttgccggttt
     6481 tgccgcagcc cagcttctgt gggggccact ctccgaccgt tatggtcgta aaccggtgtt
     6541 attaatcggc ctgacaattt ttgcgttagg tagtctgggg atgctgtggg tagaaaacgc
     6601 cgccacgctg ctggtattgc gttttgtaca ggctgtgggg gtctgcgccg cggcggttat
     6661 ctggcaagca ttagtgacag attattatcc ttcacagaaa gtcaaccgta tttttgcgac
     6721 catcatgccg ctggtgggtc tgtctccggc actggctccc ctgttaggaa gctggctgct
     6781 ggtccatttt tcctggcagg cgattttcgc caccctgttt gccattaccg tggtgctgat
     6841 tctgcctatt ttctggctca aacccacgac gaaggcccgt aacaatagtc aggatggtct
     6901 gacctttacc gacctgctac gttctaaaac ctatcgcggc aacgtgctga tatacgcagc
     6961 ctgttcagcc agtttttttg catggctgac cggttcaccg ttcatcctta gtgaaatggg
     7021 ctacagcccg gcagttattg gtttaagtta tgtcccgcaa actatcgcgt ttctgattgg
     7081 tggttatggc tgtcgcgccg cactgcagaa atggcaaggc aagcagttat taccgtggtt
     7141 gctggtgctg tttgctgtca gcgtcattgc gacctgggct gcgggcttca ttagccatgt
     7201 gtcgctggtc gaaatcctga tcccattctg tgtgatggcg attgccaatg gcgcgatcta
     7261 ccctattgtt gtcgcccagg cgctgcgtcc cttcccacat gcaaccggtc gcgccgcagc
     7321 gttgcagaac actctgcaac tgggtctgtg cttcctcgca agtctggtag tttcctggct
     7381 tattagtatc agcacgccat tgctcaccac caccagcgtg atgttatcaa cagtagtgct
     7441 ggtcgcgctg ggttacatga tgcaacgttg tgaagaagtt gactgcccga atcatggcaa
     7501 tgccgaagtc gctcatagcg aatcacactg atctatatcg atatacttat acttaggctg
     7561 ctaacaaaat tttgttgtat gattgaaatt agcggcctat actaatttcg agttgttaaa
     7621 gctacgataa atattatgtt tttacgggga caggatcgtt cccgactcac tatggatggt
     7681 catttcggca agggttcctc ctttccctct gttctacgtc ggattataga ctcgcggttt
     7741 tttctgcgag atttctcaca aagcccaaaa agcgtctacg ctgttttaag gttctgatca
     7801 ccgaccagtg atggagaaac tatgagttca tcgtgtatag aagaagtcag tgtaccggat
     7861 gacaactggt accgtatcgc caacgaatta cttagccgtg ccggtatagc cattaacggt
     7921 tctgccccgg cggatattcg tgtgaaaaac cccgattttt ttaaacgcgt tctgcaggaa
     7981 ggctctttgg ggttaggcga aagttatatg gatggctggt gggaatgtga ccgactggat
     8041 atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc aactccccca tcatttcaaa
     8101 gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc agagtaaaaa acgtgcctgg
     8161 atagtcggca aggagcatta cgatttgggt aatgacttgt tcagccgcat gcttgatccc
     8221 ttcatgcaat attcctgcgc ttactggaaa gatgccgata atctggaatc tgcccagcag
     8281 gcgaagctca aaatgatttg tgaaaaattg cagttaaaac cagggatgcg cgtactggat
     8341 attggctgcg gctggggcgg actagcgcac tacatggcat ctaactatga cgtaagcgtg
     8401 gtcggcgtca ccatttctgc cgaacagcaa aaaatggctc aggaacgctg taaaggcctg
     8461 gatgtcacca ttttgctgca agattatcgt gacctgaacg accagtttga tcgtattgtt
     8521 tctgtgggga tgttcgagca cgtcggaccg aaaaattacg atacctattt tgcggtggtg
     8581 gatcgtaatt tgaaaccgga aggtatattc ctgctccata ctatcggttc gaaaaaaacc
     8641 gatctgaatg ttgatccctg gattaataaa tatatttttc cgaacggttg cctgccctct
     8701 gtacgccaga ttgctcaatc cagcgaaccc cactttgtga tggaagactg gcataacttc
     8761 ggtgctgatt acgatactac gttgatggcg tggtatgaac gattcctcgc cgcatggcca
     8821 gaaattgcgg ataactacag tgaacgcttt aaacggatgt ttacctatta tctgaatgcc
     8881 tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc aggtggtgtt ctcacgcggt
     8941 gttgaaaacg gccttcgagt cgctcgctaa aggctattct atcgccccct cttcgggggc
     9001 gagttcagat caggcttctg tacctggttg attcatggca ttttctcgtg ccgccagcac
     9061 acgttctacc gtatctacca ctgcctgagt ttgtggatcg atttcaatgt tgacgcgtgc
     9121 gccaagtttt ttcttcccaa gagtcgtgcg ttccagtgtt tccggaatta aatggacgca
     9181 aaaacgcgtt ggcgtgactt cgccgacggt caggctaata ccgtcgatgc caataaatcc
     9241 tttgtacagg atatatttca tcaactgact atcctggact ttaaaccaga tctggcgatt
     9301 attttccgag gttaatattt tcgccacttc agcggtggtc ataatatgac ctgacattaa
     9361 gtgtccgcca atttcatcac tgaatttcgc cgcacgctca acgtttaccc aatcccccac
     9421 ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc atcaggtcaa aactgacatg
     9481 gttgccgtta atttccgtca cggtcaggca gcaaccgtta tgcgccacgg aagcaccggt
     9541 ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca tgcgtacgaa aatttggttt
     9601 ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc cccgtaaaca tacttacaac
     9661 tcctgaaatc agttaagaca ttctgttcag cacaatagca ggtggaaaac gcccttacca
     9721 gtgaaggggt aagaatggct attttttcac tggagaatta ataaatcctc gctacaatag
     9781 actgaatttc ccctgcttct tctttttgct gcccattcag gcggcttttt agtctctcat
     9841 ataactacaa ataaaaggtg ttcacgtgca gaagtatatc agtgaagcgc gtctgttatt
     9901 agcattagca atcccggtga ttctcgcgca aatcgcccaa actgcgatgg gttttgtcga
     9961 taccgtgatg gcgggcggct atagtgccac cgacatggcg gcggtcgcta tcggtacttc
    10021 tatctggctt ccggcgatcc tctttggtca cggactgctg ctggcattaa cgccggttat
    10081 cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg catcaggtgc gacaaggttt
    10141 ctggctggca ggttttgttt ccgttctcat tatgctggtg ctgtggaatg caggttacat
    10201 tatccgctac atggaaaaca tcgatccggc tctggcggac aaagccgtgg gttatctgcg
    10261 tgcgttgttg tggggcgcgc cgggatatct gttcttccag gttgcccgta accaatgtga
    10321 aggtctggca aaaaccaagc cgggtatggt aatgggcttt atcggcctgc tggtgaacat
    10381 cccggtgaac tatatcttta tttatggtca tttcggtatg cctgagctcg gtggcgttgg
    10441 ttgtggcgtg gctactgcgg cggtgtattg ggtcatgttc cttgccatgg tttcttacat
    10501 taaacgcgcc cgctccatgc gcgatattcg taacgaaaaa ggcaccgcta agcccgagcc
    10561 tgcggttatg aaacgactga ttcaactcgg tttgccgatt gcgctggcac tgttctttga
    10621 agtgacactg tttgccgtcg tggctctgtt agtgtctccg ctcggtattg ttgatgtcgc
    10681 aggacaccag attgccctga actttagttc actgatgttt gtgcttccaa tgtcgctggc
    10741 ggcagcggta actatccgcg taggttatcg tctgggtcag ggctcaacgc tggatgcgca
    10801 aaccgctgcg cggaccgggc ttatggtggg tgtctgtatg gcaaccctga cggccatttt
    10861 cacggtttca ctgcgggagc aaatcgccct gttgtacaac gacaatcccg aggttgtaac
    10921 gctggctgcg catttgatgt tgctggcggc ggtatatcag atttctgact caatccaggt
    10981 gattggcagt gggattttgc gtggttataa agatacgcgt tccattttct atatcacctt
    11041 tacagcttac tgggtgctgg gcttgccaag cggctatatt ctggcactga ccgatctggt
    11101 cgttgaacct atggggccag caggcttctg gataggcttt attatcggcc tgacatcggc
    11161 agccattatg atgatgctgc gtatgcggtt cctgcaacgt atgccgtcag ccatcattct
    11221 gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc acgggttgcg cctgtgtttt
    11281 tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg gctgtaattt cagagcgtta
    11341 cacagagttt gccgactggc atctgttatc tcaacgcgta tatccccgtt aacttcccct
    11401 ttcacattga tgaagtcagg tgaaatttct ttcgcactgg tttgcaggca aatcgctccc
    11461 tctccgcgca ggcgtttcac tttcagttgc acatgcgttg ccgcaccagt gatcagccac
    11521 agttttccgc cattcagatt gatgttacca acaatgaaag tgacgctgtc accttttacc
    11581 gtactgccgt cgagaatcaa cacaccgccc tgctgtacag taatcgttga ttgttgcagc
    11641 tctgcgccat aaccaataat cattgaagcg ccgtccagta atgcaacact cccggtcagt
    11701 tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt ccgcgctggt gggtgacaac
    11761 aggatcactg ttccaccttg tattaccgca ttttccagcg taccggcatg cacttcagcc
    11821 cgccccccca cattcaccga caggttttgc gtcttaccgg aactgtagag ctgaaggcca
    11881 tccgtcccca gacgataaat ggatccattc tcaacaatag tgtcagagtc ttcaccaccg
    11941 agattattca tggtgccacc agcaagcaac gtatcactgg ctttcccggc cagcataaaa
    12001 acgccgccct gattaatctc acaattgctg gcttctccac ttggatacac caacaattca
    12061 ccaccttcat ctaccacgac cgctgaggtt gtcccattaa ccaacaggcc gccctcttga
    12121 tcgagaatga ttttttccgc gcgatgtcct tccagtacac gcaggttacc gccattttcc
    12181 agcaacaaac cgcaggcata accctgatca acgctgaatt caccttcggg atggcggcca
    12241 ttaaccgtag cgagcgtaga gagactaatt gcgcctccct cggactgaac aatatctgtg
    12301 gcaatggcac cgtcgctgac cgatagcgtg ccattgttac cgtactgtgt gcatttggcg
    12361 atcccaccgg ttgccacttt caaccagcca gcccccgtga ccacggtatt ttcggctctt
    12421 ccgccagctt caacaatcaa ctcgccttca gtaagttgcg tcgcgccaag gacctcgccg
    12481 gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt tcgcatcaga tcccatgatt
    12541 tattcctttg ctgcatctgt gtgcctttat tgctacctaa gtgtaaaggc tacggaggat
    12601 ttatccacga cagatttgag atggtggcaa acaactctgt ttaaactctg atacacgaat
    12661 tattgggttg tatcagatgt aaatgcgatc ctgaataaaa atcgcccttg caaatcaaca
    12721 aaatatagcc aatttgctta aaagctctgc aaacggtcga aatagcgcag aaaattacgt
    12781 tttgcctctt gccacctttc cactctgccg ctaatattcg tccccgttgt cacctacaac
    12841 gttgcgttca tagctcagtt ggttagagca ccaccttgac atggtggggg tcgttggttc
    12901 gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt tggttagagc accaccttga
    12961 catggtgggg gtcggtggtt cgagtccact cggacgcacc agattttctt aatctggtct
    13021 tttccttttt ccctctgttt cttctctgta tccaatacgt taaaagattt acactgtctt
    13081 cgtatgcgtt atcagaagga gaatcgctat ggcaactttg ttacaacttc attttgcttt
    13141 taatggccca tttggcgacg caatggctga gcagcttaaa ccacttgctg agtcgattaa
    13201 tcaggaacct ggttttctgt ggaaggtatg gacagaaagt gaaaagaacc acgaagccgg
    13261 tgggatctac cttttcactg atgaaaaaag cgcccttgcc tatctggaaa aacatactgc
    13321 ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa gttttcgatg tcaatgagcc
    13381 acttagtcaa atcaatcagg caaaactcgc ctgacagaat ttaatcaagg gcggttagcg
    13441 cccttttcat ccctgtctga aatttctcaa attctaaaaa tctcaaccaa acttatctga
    13501 taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa tgaaaaaaat tgctattgtg
    13561 ggtgccgggc ctacggggat ctacacctta ttctcgcttc tacagcaaca aactccactt
    13621 tctatttcta tcttcgagca ggctgacgag gccggtgtcg ggatgccata cagtgatgag
    13681 gaaaactcaa aaatgatgct ggcaaatatt gccagtattg aaataccgcc gattaattgt
    13741 acgtatctcg aatggctaca aaagcaagaa gccagccatc tccagcgtta tggcgttaaa
    13801 aaagaaacct tgcacgatcg tcagttttta ccgcgaattc tgctgggcga atatttccgc
    13861 gatcaatttt tacgattagt agaccaggca cgacagcaaa aatttgcagt ggctgtttat
    13921 gaatcatgcc aggttaccga tctgcaaatt acaaatgctg gcgtcatgct cgctacaaat
    13981 caggatttac ccagcgagac gtttgattta gcggtgattg ccacgggtca cgtctggcct
    14041 gatgaagaag aagcaaccag aacgtatttt cccagcccgt ggtcaggcct gatggaagca
    14101 aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct tgagcggact ggatgcggca
    14161 atggcagtgg ctattcagca tggttcgttc attgaagatg ataaacaaca cgtcgttttt
    14221 caccgcgata acgcaagtga aaagctaaat attacgttga tgtcgcgcac gggtatttta
    14281 cccgaagccg atttctattg ccctattccc tacgagccct tacacatcgt caccgatcag
    14341 gcattaaatg ctgagattca aaaaggtgaa gagggccttt tggatcgggt atttagattg
    14401 atagtagagg aaatcaagtt tgctgatcca gactggagcc aacgcatagc cttagagagc
    14461 ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc gcaaacaacg cgacccattt
    14521 gactgggcag aaaaaaatct ccaggaagtc gaacgcaata aacgagaaaa acatactgtt
    14581 ccctggcgtt atgtcattct gcgcctgcat gaagccgtac aggaaattgt tcctcatctg
    14641 aatgaacacg accataaacg gttcagtaaa ggccttgcca gggttttcat cgataattat
    14701 gcggcaatcc cttcagagtc tattcgtcga ctactggcct tacgtgaagc ggggatctga
    14761 aatccctgat gttggcgaag attatacgtt acagcaaccc gaagatattc gtggacgcgt
    14821 agcgttcggc gcgttgccct ggttgatgca cgaccagcct ttcgttcagg gacttacggc
    14881 atgtgcagaa attggtgagg cgatggctcg ggcggtcgta aagcctgcat cccgtgctcg
    14941 tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact gatgctgttc
    15001 attaagacga tagcggactc cggttcgaat attgttttca ccttcaatgg cgacggcgaa
    15061 gcgaactctt tcgccgtcat gatatgccag tgcggcgctt ccccctagcc cgagaataat
    15121 tgagtcgacc acgcccgtgc tcactaccgt tgaattttca ccgctggcaa tgatgtgtac
    15181 attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt gcaccaggtc
    15241 accattactg gcgacatgac accgttcacc caatgtacaa acgcgtactc gcattccggt
    15301 attcgcaatc cgactactat ctccagcgct gcttatccgc gaagaattac cggttacagc
    15361 aacccgtgcg cgttcaccgg agctgccaat gtgactgtta aagccgacac ttccaatccg
    15421 cacgctataa cccatactgg caatttgcgc ggcataccca gcggttgcga ttcgggcgtt
    15481 atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac tgcaaaatgg
    15541 tatctctccg gggtctgtgg ccagtttttg catagaggaa acttcctgat gcgcgaagtt
    15601 ttcatccgcc agccattttt tccacgcata ctcaaccaga ctctccgccc agtccgtata
    15661 tccttcctca atcagtgcat gacgaatatc ggcataacta ccgccgcgtg ggaaatggcg
    15721 caggaaccag cgatacatca ccgctccgat acgccattct cgcaggtctg ctcgtgtaat
    15781 gatcattcgt tagtgccggt gatagccatc gaccatgctt ttaaaggttt cgtgtggcgt
    15841 acgccccgtg gtgcaaagat aaagatgacc gaagataaaa aagaggctta taaatgccag
    15901 agcaaaatgc gcctgcaata accagtatct tacgccagga aacacatctc ccacggcttg
    15961 cggatagaga cacaacagcc ccgtcaatag taacaacggc agcaatccat acatgacacc
    16021 aacataggcg acctgctgta agggattaaa tttagactgt gttgttgccg ggaaaggatg
    16081 ttcttccccc tgcataatgc caaacaaata aaatcgcgtt tgttttgccg ctcgttccag
    16141 ccacccctga cgacgaatgc gatagtggtg accattatcc ccaacggcat tgatcagcac
    16201 aaagccgagc cagcatgcca gtaacaaaaa tccgcaaact tcatgcaccg caaccagact
    16261 tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc agcccacttg ccagcaacaa
    16321 tacaaagagt aacgcattcg accagtgcca taatctgacc gctttgctgt aaagataaac
    16381 cttctcacca tgatcggtgg ctgatttctt cacgccacga gcacgaagca aggcgtgcag
    16441 ccccagcacc aaccacatcc caaccagcaa tactccggca atgatcaacc acaccggcca
    16501 aaattcggga gtgaactgtg gcacatagtt cgctaactgg ctctgaaact gttcagcatg
    16561 ttgcgacggg ttcatacatt ttccttttta atcaaatgtt gaccgaaccg gcgatacaga
    16621 tgcggttttc cggcaccagg tagctgatat tgatagtatt tattatcctg caaccacgcc
    16681 tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt gttccggaca ggcgctgacg
    16741 caaatgggcg gaaagccttt tgccagtcgg gactcagcgc agaaatcaca tttatctgcc
    16801 actttagtga cagggttaag gtagcgaacc aggtacggac aagcgccaat gcaataactg
    16861 cagccgatgc actgcgattt ttctacccgc acgatcccct gctcatcacg ccacgatgcc
    16921 cccgtcgggc atacgtcgat gcacggagca tcttcgcagt gctgacatga ctggcgaaaa
    16981 aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat cgataaacgg
    17041 cttccctgag cggggacgtg gttagtttta cggcatgcgc gggcgcaaat attacagcca
    17101 ttgcaccgtg actcgtcatg aatcatggca taccgaactt ccttttcttg cctcgtgttc
    17161 gctaacagag aagacgcaga accagtaaaa aagattactg ttcccatccc cagaacaaat
    17221 ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag tggctgcaac tgtccaccct
    17281 atccatgaca tctccctgaa atttcagggg ttctgaaact gctctacctc gaaccattcg
    17341 cataagccat aagccttgcc ataggtcata aatcgctcgc taaacaagta caacaccgcc
    17401 gtcgatgact ccacctgccg tatatcggca tattcaggtg caacatccat cgcggcgatc
    17461 gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat ggcgactttg
    17521 taaggacgag gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc gcgacaaatg
    17581 tcctgctcca caacttgcaa agacatcgcc gcgtagtttt cactcatggc ctgcgttgag
    17641 taatagtaat catcctgact tcccgtcata caagcgatat ccgcgtagtc tccgttctgc
    17701 ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa ccgaataggg tggcgagaga
    17761 aacacggtgc gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa ctcagccagg
    17821 atttcagcag gttctggttc ggttaccgta tcaacggggt gacgcatccc ctgcaaccat
    17881 tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc ctgatggttc
    17941 atcttaccca ttccttacgc aggcagtaga ttgtgtgctg ccagatcggc ggcaatatct
    18001 tccagcccca gacgctgcaa tgtttcgcgg gttggacaac caagctgtgg gtcccagccc
    18061 atttctttgt agaacatcgt gagcgaagca tgcatatcgt cacgatccat tttgtcagta
    18121 ccttcagtaa agaccgggat ctgcggatcc ttgtcgaaaa cccaggaaca gataagatcg
    18181 tgttcattac gcatatcttt ggtttgcatc ggttttaccg tgtaggcacg atgcaacgta
    18241 aagatacgct ctgcagctaa atctaatttt tcctgagtca tctcttcgcc ggtgatcgct
    18301 ttgaagaatt tggcttcaag cgccagatcg ccccggtaat tacggctttt cagcggcgaa
    18361 acggtcattg gccagaccca gttgcacaga gtgacggcgt tatgcaaaca gacccgcaac
    18421 agcgaccact ttgcatattt aatttttgcg tcattgattg gcgtgtagtt tttggtttca
    18481 tcgtaagcat cttcagaacc aaaaagttct ttcgccactt cacgttgcag tttcaatggc
    18541 aagccggaac caataaagtt gatatgggta tgcgtcatgc agtcacggtt gaacatacac
    18601 ttaacaatgg aaccgacctg cgctgacgct tcattggcat ggtgaaccgg atagccaaac
    18661 ggcgaccaga gtttattttt cgcgtagccc cagtactctt tacccaaatt ccagcgttct
    18721 gcgatggcat atgaaccatc agccaggtga ctcagctcac ccacacgatg cgccagacgg
    18781 tagtaaaaat ctttaatgaa gttaacgtca cccgcttcca gttgatccca gcgaatttct
    18841 gcatactctt cagctggcag aacacgcttg aacacacctt tgctgtaaca ataagtaaag
    18901 tcgcgatgca actgcccgta gttacaccat aggccgtagt catcgaacaa attcagacca
    18961 accaggttac caatcacacg accatcgtct ttatcttcaa aatctttcgg cccgttcggg
    19021 aagatggtgg tatggacaaa gtttgctaca caagtgttac cacctgtgct gggcacgcca
    19081 aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca agagtggcag
    19141 ccgctcattt ttactgtgta tttctctgcc gccggtccta agtcaaaaac ggatttatag
    19201 gtacgaaagc cgaccgtatt ctgattgcct ggcggaattt cacccgtttc aatcggacca
    19261 ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc agcgtgactt gggatctgcg
    19321 tacttggcac gacatggttg ttattcgcac caataagttc agtcatcatg taatcattga
    19381 gacgcttcat ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg
    19441 ctttcaggtt tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc
    19501 tattcaacat gccagagaga ggaataaggt tttccccagc ctgaccaata gccgccacac
    19561 aggtttccgg gctggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc
    19621 ataagaaatc ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg
    19681 atttcgcctt cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa
    19741 aaaagccacc catatgggca tcgcgactaa atttcctttg gtaaaagtag aaagtgaggt
    19801 gatatttacg cgagaactac agggggcacc agatccaggt aatgggccgg tagcaaagac
    19861 taatttattc gcttcatcga aaggtttcgt gcctagcggt acttcgtcat acataatttt
    19921 gtagccgaag cccatgccac cgacaaaact tttaaactta ctggaatctt cgagggtaat
    19981 atttcctgtc gtgagattga ctcttaatat attacctgtc caaccgttag ccatgatttt
    20041 ttcctttgca agattcacac agtaatatct ttccactcga taatttttaa cgcccctgtt
    20101 gggcaggcgt ttgcgcattc accgcataac acacatttcg aggatttttt actttcggta
    20161 tttacggtgg ccatcatcca cgggcatgcc gtagtacagg cgctacagcc aatacaacgt
    20221 ttatgatcga cggtaataca gccttctttc tgctgccagg taatcgcacc aatcgggcag
    20281 acgttcatgc attgcggctc tttgcattga cggcaggtgt ccgcggtata gttgagatcg
    20341 ccatacaggc cgccgccaga gccaaccccg ttgtcgccaa agaaataatt gcgatggatt
    20401 ttgatacggg agaagaatgt ccctactgag ccatcgttga agttggtaca agagatttca
    20461 cagcggtggc aacccgtgca tcgcgctcgc tgggtcacca gcaccccttt gggtgtattt
    20521 atcaacccta ctgtgccgct atcaatatct tcctgtttgc aacccaaaag tgacagtaac
    20581 gcaggggcaa ttgttagacc agcaaggcct tttcctgata tacgcaagaa ttctaatcgc
    20641 gttaagccaa tatctaatag tggacgatca accgggttca tttatttcgc atcctcttcg
    20701 caaacacagt gctgacaatt cagcagcctg atattaagca ttcaactaat acgctgctga
    20761 ctctgtcccc aacaccagat attttcccta acccgatagt gtatgagaaa agatttaacg
    20821 ccgataaact aatcctttat ggttaggaga aaaaataatg tgattattct gagcccttaa
    20881 cattgatcgt tatcaattaa aattacaaag caagaaatta taagcgaact gatatttatt
    20941 atcatttgaa ataaatttaa cttaagcgac aatcatttgt gacgttcatt gcagaaaata
    21001 aaaataacat tatcgctata aattaaaata cataccgaaa ttcgtataat ttcagcagtt
    21061 gttatttaga atgattaact ggaccgcaac tgaagcaaga atatcaggat gaagcttacc
    21121 gcctcatcct gaatggtcat tttaccagtg cgttaatgac ggtttccatc gcctgctttt
    21181 ccagctcact gcgctgaatg cgtttattgg ctaatgctgt acgtaatacg cctgcgataa
    21241 caatatgctt tggttcctgc cccagatcgc gcatttccag cacaacttta ccgacaactc
    21301 tgcacatttc tcggtacagt tcatcctctt ttgtccgatt tcccattctt tatacctatt
    21361 tatcatgcca acta
//
LOCUS       DAJTYH010000067        21224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTYH010000067 DAJTYH010000000
VERSION     DAJTYH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO609_002534"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO609_002534"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6633908.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="OO609_002535"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="OO609_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6633909.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="OO609_002536"
     CDS             complement(2650..3201)
                     /locus_tag="OO609_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6633910.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="OO609_002537"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="OO609_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6633911.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="OO609_002538"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="OO609_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO609_002539"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO609_002539"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6633912.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO609_002540"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO609_002540"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6633913.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO609_002541"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO609_002541"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6633914.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO609_002542"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO609_002542"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6633915.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO609_002543"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO609_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6633916.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="OO609_002544"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="OO609_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6633917.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="OO609_002545"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="OO609_002545"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6633918.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO609_002546"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO609_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6633919.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO609_002547"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO609_002547"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6633920.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO609_002548"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO609_002548"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6633921.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO609_002549"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO609_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6633922.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18171..18566)
                     /locus_tag="OO609_002550"
     CDS             complement(18171..18566)
                     /locus_tag="OO609_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6633923.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO609_002551"
     CDS             complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO609_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6633924.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19482..20075
                     /locus_tag="OO609_002552"
     CDS             19482..20075
                     /locus_tag="OO609_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6633925.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20220..20672
                     /gene="tabA"
                     /locus_tag="OO609_002553"
     CDS             20220..20672
                     /gene="tabA"
                     /locus_tag="OO609_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6633926.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20795..>21224
                     /locus_tag="OO609_002554"
     CDS             20795..>21224
                     /locus_tag="OO609_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6633927.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQS"
BASE COUNT         5087 a         5286 c         5607 g         5244 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgtgac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaagcggtg
    17941 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat
    18001 atatgcattt ttattttcta tgattctgga ggggaagtgt cgttcggtat caataattgc
    18061 agacatttcc tgcttttttc tttttcattt tcacgcaatc agaaatgcat tttattccat
    18121 tcatccgact tatagggcgg agggtgaaaa cgaacggcta acactattgc ttatagctca
    18181 gggatacgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacacccacc
    18241 gccgtccagt ttggataagg tggggcgcta aatatttcat ttttcaccgt catgatgtct
    18301 tcagattgtt tttccggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    18361 catcccgcag ctgccagggt cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga
    18421 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    18481 tcgccggaac gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca
    18541 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    18601 gatttatatt tatcgggagc cgcttttagg gggacgacgt tagtaaacaa aacccagaca
    18661 tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac gcgccatcaa
    18721 tggtatgcat cgcgccggta acaaaactgg cttctggccc tgctaaccat gcgaccatac
    18781 cagcgacctc ttccggttgc ccatgtcttt tgatggccat aaaactatgc aacatatcgc
    18841 gcattggccc gttggcggga ttaacgtcgg tatcaattgg tcctggctgg acgacgttga
    18901 tggtgatccc tcgcggtcca aaatcacggg ccagcccgcg cgccatgcct tgcagggcag
    18961 atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca ttcacggagc
    19021 cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaaca gaggcatgat
    19081 aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt aattccaggg
    19141 catcgccaaa gacgccaata cctgcattta ccaccaggat atccaatgcg ccgctcttac
    19201 gaacgacatc aataacagcg tctctgtcag cactatctgt gaatactgct gtcgctccag
    19261 tctcttgtgc cagacgttca gcggcatctt tcgaccccgc ataggtgaat cgtacattgg
    19321 ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacgactg ccaccgagga
    19381 tgagaactgt cttaccggta aaagcgccca taaggactcc ttgatttatt atgtaacatg
    19441 cattacaaaa ctgttttaac tttctgtcaa caggttttgt aatggtcact aaaaaacaat
    19501 ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct gcggcaaaag
    19561 tgctttttca ccaaaaaggt ttcgatgcag taagtgtctc tgaagttact gattatcttg
    19621 gtattaaccc cccgagtctc tacgcggctt ttggcagtaa ggctgggtta tttagccgtg
    19681 tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt cgtgatgatc
    19741 gtccggtagg cgagtgcctg gttgaggtat taaaagaagc ggcgcgcaga tatagccaaa
    19801 acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat ccacaagcgc
    19861 gtgatattgc cattcaatat tatcacgccg cagaaacgac catttatgac tatatcgcca
    19921 ggcggcatcc acaaagcgca caatgtgtga ctgattttat gagtaccgtg atgtcagggc
    19981 tttctgcgaa ggcacgggag gggcactcca tcgaacaact ctgtgcaaca gctgccctgg
    20041 caggggaagc gataaaaact cttctcaagg agtgatgctg gccttgatcc gaaaggcggg
    20101 aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc tggcggaatt
    20161 gatcatcttt tcgtataacg atagaaatga aacattgttt taattaagga gtggaaaaga
    20221 tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta cgccaggcga
    20281 ttgagcatat caaagcacac gttacggcag aaacgccaaa gggcaagcat gatatcgaag
    20341 gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa gctcgccgtg
    20401 cggagtacca tgcccgctat cttgacattc agattgtgtt aaaaggtcag gaaggcatga
    20461 ccttcagcac gcaacctgca ggcgcgccgg ataccgactg gttagctgat aaagacatcg
    20521 catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt gattttgttg
    20581 tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggcgcgcca gcccaggttc
    20641 gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat aactccaggt
    20701 ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa aatttgtaat
    20761 gcttagacgt tcttattaac tcaaggagtt cgtcatgggc aaaatatcag atttgaatta
    20821 ttctcaacac attacattag ccgacaattt taaacaaaaa aatgaagcct tagatacctg
    20881 gtatgtggga atgaatgatt ttgcccgaat tgccgggggg cagaatagca gaagcaatat
    20941 tctttctcct agagcatttt tggagttttt ggcaaagata tttaccctgg gttatgtgga
    21001 ttttagcaaa cgctccaacg aagcgggtag aaatatgatg gctcatattg agttctcatc
    21061 ttattctaaa gatactgatg gcaatgaaaa aatgaagttt tacatgaata atcctgaagg
    21121 ggagcgagcg gatttatcta aggtgaagat agaaattaca cttgcctcag cttctactaa
    21181 agggattcgt gaaggacata cagtcattat atttaaacaa tctg
//
LOCUS       DAJTYH010000068        20852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTYH010000068 DAJTYH010000000
VERSION     DAJTYH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..525
                     /locus_tag="OO609_002555"
     CDS             <1..525
                     /locus_tag="OO609_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6633928.1"
                     /translation="NVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVI
                     SYSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSM
                     SRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGL
                     TPIEYRNQTYMPRV"
     gene            627..779
                     /gene="hokA"
                     /locus_tag="OO609_002556"
     CDS             627..779
                     /gene="hokA"
                     /locus_tag="OO609_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6633929.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(966..1178)
                     /gene="cspA"
                     /locus_tag="OO609_002557"
     CDS             complement(966..1178)
                     /gene="cspA"
                     /locus_tag="OO609_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6633930.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1459..1749)
                     /locus_tag="OO609_002558"
                     /pseudo
     CDS             complement(1459..1749)
                     /locus_tag="OO609_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2183..2893
                     /locus_tag="OO609_002559"
     CDS             2183..2893
                     /locus_tag="OO609_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6633931.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2943..3917)
                     /gene="ghrB"
                     /locus_tag="OO609_002560"
     CDS             complement(2943..3917)
                     /gene="ghrB"
                     /locus_tag="OO609_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6633932.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4021..4671)
                     /locus_tag="OO609_002561"
     CDS             complement(4021..4671)
                     /locus_tag="OO609_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6633933.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4834..7032
                     /locus_tag="OO609_002562"
     CDS             4834..7032
                     /locus_tag="OO609_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6633934.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7001..7441)
                     /locus_tag="OO609_002563"
     CDS             complement(7001..7441)
                     /locus_tag="OO609_002563"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6633935.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7438..8001)
                     /gene="tag"
                     /locus_tag="OO609_002564"
     CDS             complement(7438..8001)
                     /gene="tag"
                     /locus_tag="OO609_002564"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6633936.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8159..8857
                     /locus_tag="OO609_002565"
     CDS             8159..8857
                     /locus_tag="OO609_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6633937.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9086..10294
                     /locus_tag="OO609_002566"
     CDS             9086..10294
                     /locus_tag="OO609_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6633938.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10618..12309
                     /gene="eptB"
                     /locus_tag="OO609_002567"
     CDS             10618..12309
                     /gene="eptB"
                     /locus_tag="OO609_002567"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6633939.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12401..12477
                     /locus_tag="OO609_002568"
     tRNA            12401..12477
                     /locus_tag="OO609_002568"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12435..12437,aa:Pro,seq:cgg)
     gene            13221..14828
                     /gene="dppA"
                     /locus_tag="OO609_002569"
     CDS             13221..14828
                     /gene="dppA"
                     /locus_tag="OO609_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6633940.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15136..16155
                     /gene="dppB"
                     /locus_tag="OO609_002570"
     CDS             15136..16155
                     /gene="dppB"
                     /locus_tag="OO609_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6633941.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16165..17067
                     /gene="dppC"
                     /locus_tag="OO609_002571"
     CDS             16165..17067
                     /gene="dppC"
                     /locus_tag="OO609_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6633942.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17078..18061
                     /gene="dppD"
                     /locus_tag="OO609_002572"
     CDS             17078..18061
                     /gene="dppD"
                     /locus_tag="OO609_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6633943.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18058..19062
                     /gene="dppF"
                     /locus_tag="OO609_002573"
     CDS             18058..19062
                     /gene="dppF"
                     /locus_tag="OO609_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6633944.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19092..20363)
                     /locus_tag="OO609_002574"
     CDS             complement(19092..20363)
                     /locus_tag="OO609_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6633945.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5047 a         5123 c         5459 g         5223 t
ORIGIN      
        1 aatgttctcc aaagagattt caaggctacg cggccaaacg agaagtgggt taccgatgtt
       61 actgaatttg cagtcaatgg gcgcaagctg tatttgtctc cagtaataga tctcttcaac
      121 aacgaagtta tttcttacag cctttcggaa agaccagtga tgaacatggt tgagaatatg
      181 ctcgatcagg cattcaaaaa gcttaatcct cacgagcatc ctgttctgca ctctgaccag
      241 ggatggcagt atcgtatgag aagatatcaa aatatcctta aagaacatgg tattaaacaa
      301 agcatgtcca gaaaaggcaa ttgtctggat aatgctgtgg tggagtgttt ctttggaacc
      361 ttaaagtcgg agtgttttta tcttgatgag ttcagtaata taagcgaact gaaggatgct
      421 gttacggaat atattgaata ctataacagc agaagaatta gcctgaaatt aaaaggtctg
      481 actccaattg aatatcggaa tcagacctat atgcctcgtg tataactgtc caactttttg
      541 gggtcagtac aaagtgaggc taccctatgc ctgaacacca taaggttagc ctcttactcg
      601 ttggaaatca acacaggggg ctgggaatgc cgcagaaata tagattactt tctttaatag
      661 tgatttgttt cacgctttta tttttcacct ggatgataag agattcactg tgtgaattgc
      721 atattaaaca ggagagttat gagctggcgg cgtttttagc ctacaaattg aaagagtaag
      781 agtcttcgac gggaaattat tcccgcctta cttacggcgt tgcgcattct cattgcaccc
      841 atatttattc ttcacaaaaa taataataga ttttattacg cgatcgatta tttatttcct
      901 gaaaaaaaat aaaaaaatcc ccgccaaatg gcagggatct tagattctgt gcttttaagc
      961 agagattaca ggctggttac gttaccagct gccgggcctt tagcgccgct ttcgatggtg
     1021 aaggacactt tctgaccttc gtccagagat ttgtaaccat cgttctggat agcagagaag
     1081 tgtacgaaca catctttaga gccatcgtca ggagtgatga agccgaagcc tttgtcagcg
     1141 ttgaaccatt ttacgatacc agtcatttta ccggacatag tgtattacct ttaataatta
     1201 agtgtgcctt tcggcgatat ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca
     1261 ctacgagggg tatcaacgat aactcttgaa gggacttgcc ttactacact gctttaatgg
     1321 tctgtacgtc aaaccgttga tgtgcattaa gccactcatt ggcgggtgat gcaacaatta
     1381 tttttcatat ttatgattaa tcggttggaa aacggtcctg tcatcaggac cgtaaacagc
     1441 aataaagtgg ataaaagtct atttcatcaa ctgcttactt aatgccgggt tggcttgaat
     1501 caaacgcatc aattttagtt cggcacttga aggcttcacg cgtctggatt cccattcctt
     1561 taccatggcg actgatacgc ccaaaacccg ggcgaaatca tcgattttta atcctgtccc
     1621 ttttcataac tgctcaattt cggtacagga cgttgttctg tgcgtcaggg taattttctg
     1681 cgtttcatct ttaaaaacaa tctgttccag gctgctcaac agctcatgca ttggatcttt
     1741 atattccatt gaaaactcct caaatcacac tgcgggatcg tgaattgcat cgaagctcat
     1801 taagaatagt cgggaaaagc agacacagcg gggtgaagtc agggcatgct ctggctatga
     1861 ttcgattcag cctgtcattt agcgtgactt atagccacct aacgatctga taaatgccga
     1921 tatatctctt atcactcctg ttttgtaaag atttcgcaaa tctaaccttc tgcgaaggcg
     1981 taaatctctg gctacaaaaa aggcaacggc actatcattt tttaacaatg aaacaacaaa
     2041 ttaaccttgt ttcattttct ccatgatttt accaggaaga ttacgtagaa agggtatcct
     2101 gcaagcctgt cctggactat ccttgtcacg tcagacacgc gtgtgtcgtt gtgcgctttt
     2161 tttgggtgaa aggagtaaga aaatggcgac aggaaagtcc tgctctcgct ggtttgcgcc
     2221 tcttgcggcg ttattaatgg tagttagcct gagtgggtgt tttgataaag aaggcgatca
     2281 gcgtaaagcg tttatcgact tcctgcaaaa tacagtgatg cgtagcggtg aacgtctacc
     2341 aaccctgact gccgatcaga aaaaacagtt tggtcctttt gtctctgatt acgcgattct
     2401 gtatggttat tctcagcagg tgaatcaggc gatggattcc ggtctgcgcc cggtcgtaga
     2461 cagcgttaac gcaattcgcg taccgcagga ttacgttacg caaagcggtc cgctgcgtga
     2521 aatgaatggt tcgctgggtg tgctggcgca acaactgcaa aatgcgaagt tgcaggccga
     2581 tgctgcgcac tctgcgctaa aacagagcga tgacctgaaa ccggtatttg atcaggcatt
     2641 caccaaagtg gtaacgacgc cagctgatgc attgcaaccg ttgattccgg cggcacaaac
     2701 tttcacgcaa caactggtga tggttggaga ctacattgct cagcaaggta ctcaggtgag
     2761 ctttgtcgca aatggcatcc agttcccgac ttcacagcag gcaagtgaat ataacaaact
     2821 gattgcgcca ttaccggcac aacatcaggc gtttaatcag gcctggacta cagcagttac
     2881 tgcaacccaa taaagagtaa aagcccgagc gattatcggg ctttacttca cgcagtcgca
     2941 gcttagtccg cgacgtgagg attcacacag ttcttctcaa cctttccttg taacgcatca
     3001 atcaaattat ccacggcaca ggcggccatg ccataacgcg tctcatgggt ggcagatcca
     3061 atatgcggta ctgcgacgac gttggccatt gagagcaacg gcgaatctac ggacagtggc
     3121 tcttgttcga agacatccag cccggcggcg tgaatttcgc ctttctgcaa tgctgcgatc
     3181 agtgcatttt cgtcaaccac cgggccacgt ccggcattaa tgaaaatggc ggaggatttc
     3241 attttggcga attgttctgc gccaaacaga tgatgcgtct catcagttaa cggcaggatc
     3301 aggcaaacga aatctgactc ttgtaacaga gtatccaaat cgcagtagcg ggcgttgaag
     3361 cgttcttctg cttctttatg gtggcggcgc gcgttataga ggatgggcat gttgaagcca
     3421 aagtgcacac gttgtgccag cgccatgccg atccgtccca tcccgacaat gcccagtgtt
     3481 ttatggtgaa cgtcagtgcc gtaccagtcc gggcctatgc tcgcggtcca ttcgcctgct
     3541 tttacccgtt ctgctacctc cacaacccga cgagcggtag acaacaccag cgccatcagc
     3601 gtatcggcga cggtttctgt taatacggtt ggcgtgtgca tcagcagaat ttttcgggcg
     3661 gtaagcgcat cgacatcaaa attgtcatag ccgacggaga tcgttgatgt ggcacgcagt
     3721 ttcggcattt tttccagcaa tgcggcatct acattctcgt ttgaacccag taaaccttca
     3781 gcttctgcaa aaattgctgc attttgaacg acggtttgtg ggctgaggtt tgccacctgg
     3841 tgaacggtga aatgcgcttg caggcgttgc agtaaatcat caggtaaggc tttgtagagg
     3901 ataacggacg gcttcatgct tctctccatt cactgataag gcgatcacct tagcctgaaa
     3961 tatgatgaag gaaaactgaa gaatgccagg tgatttctgc atcacctggc atgaaaggga
     4021 ttacagcggg cttaaggtaa tttctacacg gcggttttgc gccttacctt ctgcggtgct
     4081 gttgctggcg attgggttag ccgggccaag gccctgagta cggatgcggc tggcgtccac
     4141 gccctgggtg atcaacgcgc tggcaacgga atccgcacgc tgctgggaga gacgcatgtt
     4201 caggtcgtga ccccccgtgc tgtcggtata accaatcacg ttaaccgccg ttttcggata
     4261 ctctttcagc accattgcca cgccggtcag ggtgttagcg cctgccggtt tcaggggagc
     4321 gctgctgctg tcgaaggtca cattgttcgg catattgagg ataatgttat ccccgctgcg
     4381 ggttacgcta acaccagtgc cgcgcatttt gtcacgcagc ttcgcttcct gcacatccat
     4441 gtaataaccc acgccgccgc ccagagctgc gcctgctgct gcgccaatca gcgcgccttt
     4501 accgcgatct ttcttcgaag aagagagcgc accaataccc gcgcccacga gagagcccag
     4561 acctgcgccg atagcagatt tgcctgcttc gcgttcgccg gtgtaagggt tagttgtgca
     4621 gccagatacc gccagagcgc cactcactac ggcggcaagt aagataaaca cgtttcttca
     4681 ttgttaatcc ttaataacct ttttattctt tgtcacgggt tccgtggcgg gagattatgc
     4741 cgcgtgaaca tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg
     4801 ctcaatacat ctaacctttc aggagtcttc ggtttggcca actcatcctc acgatattcc
     4861 gttctgactg ccgcccactg ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt
     4921 agctcgcgtg gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac
     4981 caggttcaca gcaatacgcg ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca
     5041 ccggaaaacc cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag
     5101 gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggtgc ggcacaggcg
     5161 atcatgccgt atgtcgtagg tggcagcgaa gtttatcaac agcaaaccag ttggccgctg
     5221 gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa tacgctgaaa
     5281 attgcgtgga atgcatccga tgagcagggg ctttcttact tttccgcact gcgtgacagt
     5341 gggaaaaagc tgatctgcat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
     5401 aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
     5461 catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg caccacaggt
     5521 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaacgccgaa
     5581 tgggcggcag agatttgtgg tgttggcgca gcgaaaatcc gcgagctggc ggctattttc
     5641 caccaaaata ccaccatgct gatggcaggc tggggaatgc agcgccaaca gtttggtgag
     5701 caaaaacact ggatgatcgt cacgctggca gcaatgttgg ggcaaatcgg cacacccggc
     5761 ggcggttttg gtctttctta ccattttgcc aatggtggtg accccacgcg gcgttctgcg
     5821 gtgctctctt ccatgcaggg cagcttgccg ggtggctgcg atgcggtgga taaaatccct
     5881 gttgcccgca ttgttgaagc actggaaaac cctggtggcg catatcaaca caacggtatg
     5941 aaccgacatt tcccggatat tcgttttatc tggtgggcgg gcggtgccaa ctttactcat
     6001 catcaggata ccaatcgtct gatccgtgcc tggcaaaaac cggagctggt ggtgatctct
     6061 gaatgcttct ggacggcggc ggcgaaacac gcggatattg ttctgcctgc gactacctct
     6121 tttgagcgta atgatctcac catgaccggt gattacagta atcagcatct ggtgccgatg
     6181 aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt tgccgagttg
     6241 agtgaacgtt gggagaaggg cggctatgcg cggtttacgg aaggaaaaag tcagctgcaa
     6301 tggctggaaa cgttttataa cgttgcccgg cagcgcgggg caagccagca ggttgaattg
     6361 ccgccatttg ctgagttctg gcaagccaac cagttaattg agatgccgga aaacccggac
     6421 agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgctggcgca tccgttaaaa
     6481 accgccagcg gcaagattga aatcttctca cagcgtattg ccgattacgg ttacccggat
     6541 tgccctgggc atccaatgtg gctggagccg gacgaatggc agggcaatgc cgaaccagaa
     6601 cagttgcagg tactttctgc ccatccggcg caccgcctgc acagccagct gaattacagt
     6661 tctctgcgcg aattgtacgc ggtagcaaac cgtgagcctg tcaccattca tcctgacgat
     6721 gctcaggcgc gcggcataac cgaaggggat atggtgcggg tgtggaactc ccgtgggcag
     6781 atccttgccg gagcggtcat tagcgaggga attaaacctg gcgtgatttg cattcacgaa
     6841 ggggcatggc cggatctgga tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac
     6901 gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg taatacgacg
     6961 ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt tgaaccaccg
     7021 gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt
     7081 gaaaaccctg cgcacggtaa aaatctatcg ccggttgatt tttttgataa acctccagca
     7141 tcagatgggg atagcgctgc tgcacatact gcatcagcgc cttaccaata ccgcgtctga
     7201 cggccttcgg tgcgacaaac attgctgcca gaaatcggcc ttccataatg ctgacaaaac
     7261 cgagaagctt accgtcctct tcccagaccc agttttgcgc gttggcaaga taggcatccc
     7321 gcaccagcgg aatgcagtca cgccagtaat tcgcttttat aaagggatgc ccccaggttg
     7381 tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct tcccgaatca
     7441 tggtttattt cccagatagc aacagcagcc aaccacatga tcattcacca gcccacatgc
     7501 ctgcataaag gagtaacaga ttgtggtgcc gacaaactta aaaccacgtt ttttcagtgc
     7561 cttagatagg gcgtcggagg cggacgtaga tgtgggaatt tcgctcaacg ttgtggcttg
     7621 tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcgggaaacg gttcgccgtt
     7681 ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt tccctcgatg
     7741 gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca ttgctgcgac
     7801 cttcaccgga tcgaactgat gaaagtaggc gcgatagttt tcgcgttttt tgaggacggt
     7861 gatccacgat aacccagcct gctgcccttc aaagcagatc atttcgaaca gttttttact
     7921 gtcagtttca ggcacgcccc actcattatc atggtaggca atataaagcg ggccctgact
     7981 cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa aaatttcagc
     8041 cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac tatgcccgat
     8101 atccttcata caatgacaat aatattgccg aattcggctc ttccctggag tcgtgttaat
     8161 gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg tggtaatcag
     8221 tgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg atacgcaacc
     8281 cggacattcc acagagcgtt acacctggga tagtgatcat caacctgatt acaacgatat
     8341 tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca atctggcgga
     8401 agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc ctttatatga
     8461 acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca cctcgatgta
     8521 taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg ccagcgtgag
     8581 tagcttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg cgcaaatcag
     8641 ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga gccggatgac
     8701 ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata tgttgctcaa
     8761 tcaaaatatt gccgcctatg ctgcgctaac tcaggcagaa aataccacta ataatagcga
     8821 ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca attgaaacca
     8881 ttgaataaca acagttgtgg cgatagtggg tgctaactta ccaaataata aatttggtga
     8941 ataattgtcg cgtcattcat tcctgaacta aggcatttca ttccgttctg atggcatttc
     9001 atgccgtttt tccccaagca taaaatgcac ttcgttatgg ttgtcggcag agatttttcc
     9061 tttttattac tgcaggaata ctgccatgac accttcaaat tatcagcgta cccgctggct
     9121 gacactcatc ggtactatca ttacccagtt tgcgctgggg tcggtttata cccggagcct
     9181 gtttaatggc gcgctttctg ccaagctgga tgcgccggta agccaggtcg ctttctcttt
     9241 cggcttgtta agtctggggc tggcaatttc gtcttctgtt gcgggcaaat tgcaggaacg
     9301 ttttggcgtt aaacgcgtca ccgtggcttc cggcattttg ctgggattag gcttcttcct
     9361 gaccgcgcat tccaacaacc tgatgatgct gtggttaagc gccggtgtgc tggtcggtct
     9421 ggcggatggc gcgggttacc tgctgacgct ctctaactgc gtgaagtggt tcccggagcg
     9481 taaaggtctg atctccgcgt tcgctatcgg ttcttatggt ctgggtagcc tgggtttcaa
     9541 atttatcgac acgcagctgc tcgaaacggt gggtctggaa aaaacctttg tgatttgggg
     9601 agcgattgcg ctggtgatga ttgttttcgg cgcaacgtta atgaaagacg caccaaaaca
     9661 ggaagtgaaa accagcaatg gtgtggtgga gaaagattac acgctggcag agtcgatgcg
     9721 taaaccgcag tactggatgt tagcggtaat gttcctgact gcctgcatga gcggcctgta
     9781 cgtgattggg gtagcgaaag atatcgccca aagtctggca caccttgatg tggtttccgc
     9841 agccaatgca gtaacggtta tttccatcgc taacctttca ggtcgtctgg tgctgggcat
     9901 tctgtctgac aaaattgccc gtatccgtgt tatcaccatt ggtcaggtga tttcgctggt
     9961 gggtatggcg gccctgctgt ttgcaccatt aaatgcagtg acgttctttg cagcgattgc
    10021 ctgcgtggca tttaactttg gcggcactat taccgtcttt ccatcactgg tcagtgagtt
    10081 cttcggcctc aataacctgg cgaaaaacta cggtgtgatt tacctcggtt tcggtatcgg
    10141 tagcattttt ggttcgatta tcgcctcact gtttggcggt ttctatgtga ccttctacgt
    10201 gattttcgcc ctgctgattc tgtcattggc gctttctacg acgattcgtc agccagagca
    10261 gaaaatgttg cgtgaggcgc atggctccct ttaatgctca gccgcgagct actgggcttc
    10321 agcttccgtg tgcatcaaac gcatcaacga aatgtataaa caatgactcg taaaatttgg
    10381 gccagttaac tggccctttt tactgctttt tgtaacgcta aagtctcttt tcaaacttgc
    10441 atttttgtaa atttgtgctt catgcacact ctttccccac actttttcgc tttgctgtgg
    10501 tctacttatt cgcgcgtgta gattttactt atctgactac ctccgcactt ttcccctgcc
    10561 gggcctgaaa agccgctaag cagggtgtta tcacctgttt gtccagggtt tgtttgcatg
    10621 agatacatca aatcgattac acagcagaag ctgagctttt tgcttgcaat ctatattggc
    10681 ctttttatga atggcgcggt tttttatcgc cgcttcggca gctatgcgca cgattttacc
    10741 gtctggaaag gcgtttctgc tgttgttgaa ctggccgcca ccgtactggt gaccttcttt
    10801 ttactacgtc ttctatcgct gtttggccgc cgcagctggc gtattctggc gtcgctggtg
    10861 gtgctctttt ccgcaggtgc cagctattac atgaccttcc ttaatgtggt cattggttat
    10921 ggcatcatcg cttccgtcat gaccaccgat atcgacctgt caaaagaagt tgttggtctg
    10981 aactttattc tctggttaat cgccgttagt gcattgcctc ttatccttat ctggaataac
    11041 cgctgtcgct acaccttgct ccgacaactg cgaaccccag ggcagcgtat tcgcagcctg
    11101 gtggtcgtcg tactggcggg tattatggtt tgggcaccga ttcgtttgct ggatatccag
    11161 cagaagaaag tggagagggc gaccggcgtt gatttgccga gttatggcgg tgtcgtagcg
    11221 aactcttatc tgccatcaaa ctggctttct gcgttggggc tgtatgcctg ggcgcgggtc
    11281 gatgaatctt ccgataataa ttcattgctt aatccggcga agaagtttac ataccaggct
    11341 ccacaaaacg ttgatgatac ttatgtcgtg tttatcatcg gtgaaaccac gcgttgggac
    11401 catatgggta ttttcggcta tgagcgtaat accacgccga aactggccca ggagaaaaat
    11461 ctggcggcgt tccgtggtta ctcctgtgat accgcaacca aactctcact gcgttgcatg
    11521 tttgtacgtc aggggggcgc ggaagataat ccgcagcgca cattaaaaga acagaacatt
    11581 ttcgcggttc tgaagcagtt aggattcagt tctgacctct acgctatgca gagcgaaatg
    11641 tggttctaca gcaacacgat ggcagacaac attgcttatc gtgagcagat tggtgcggag
    11701 ccacgtaatc gtggcaagcc ggtagatgat atgttgctgg tagacgaaat gcagcaatcg
    11761 ctagggcgca acccggatgg taagcatctg atcattctgc ataccaaagg ttcgcatttt
    11821 aactacaccc agcgttatcc gcgtagcttc gcgcagtgga agccggaatg tattggtgtt
    11881 gatagcggct gtaccaaagc gcagatgatc aactcctatg acaactcggt gacctatgtg
    11941 gatcacttta tctccagcgt gattgatcag gttcgcgata agaaagcgat tgtgttctac
    12001 gcagctgacc acggtgagtc aattaatgaa cgcgagcacc tgcacggcac gccgcgtgaa
    12061 ctggcaccgc cggagcagtt ccgcgtaccg atgatggtct ggatgtcaga taaatatctg
    12121 gaaaatccgg ccaatgcgca ggcgtttgcg cagctgaaaa aagaagccga catgaaagtg
    12181 ccacgccgtc acgtagagct gtacgacacc atcatgggtt gtcttggcta tacttcaccg
    12241 gatggtggaa ttaacgaaaa caacaactgg tgtcacatcc cgcagacaaa agtggcagcg
    12301 gctaactaag cggcctgctg actttctcgc cgatcaaaag gcattttgct attaagggat
    12361 tgacgagggc gtatctgcgc agtaagatgc gccccgcatt cggtgattgg cgcagcctgg
    12421 tagcgcactt cgttcgggac gaaggggtcg gaggttcgaa tcctctatca ccgaccaaat
    12481 tcgaaaagcc tgctcaacga gcaggttttt ttgcatttgt agctcatgag gatgagaacc
    12541 tccgggggca gggaggctcg actcgacgta ggcctgataa gacgcgccag cgtcgcatca
    12601 ggcaacggca ccgcacttat tgtcggatgc ggcgcgaccg ccttattcga ccaacacacc
    12661 acacattttc ctgccaactt tgcgccccat atctcactat ccattaacgg atttgtgaca
    12721 gaaaccattg tcttttttta tatttgctta aatctttttt gcgttactta tggctggcgc
    12781 tggcattttc cgctgtgcgt tttagcgttc atggtattaa tcgctcagat atccaccttt
    12841 tcctcagatt tttttcatta aatgcataaa acttaatcac caaatgttgc taaatattaa
    12901 gctgattgtt ctggttatgt tggagtagca caaatttctc tgctgcaaat agctgtttga
    12961 agttttttta atctttgttt gtggattctc accgttggtg taaatcccgg ttggctgtat
    13021 tgacgttttc acattctgtt gacagattgt aggtcacgag gggcatttta tggaggatcc
    13081 gcactgttac actgatgtta attagtacgg catccccacc tcataacgtt gacccgaccg
    13141 ggcaaaaaac aaaaaaggtc aggcagcgac aacccactgc aaagggttaa aacaacaaac
    13201 atcacaattg gagcagaata atgcgtattt ccttgaaaaa gtcagggatg ctgaagcttg
    13261 gtctcagcct ggtggctatg accgtcgcag caagtgttca ggctaaaact ctggtttatt
    13321 gctcagaagg atctccggaa gggtttaacc cgcagctgtt tacctccggc accacctatg
    13381 acgcctcttc cgtcccgctt tataaccgcc tggttgaatt taaaatcggc accaccgaag
    13441 tgatcccggg cctcgctgaa aagtgggaag tcagcgaaga cggtaaaacc tataccttcc
    13501 atctgcgtaa aggtgtgaag tggcacgaca ataaagaatt caaaccgacg cgcgaaatga
    13561 acgccgatga cgtggtgttc tcgttcgatc gtcagaaaaa cgcgcaaaac ccgtaccata
    13621 aagtttctgg cggcagctac gaatacttcg aaggtatggg cttgccggag ctgatcagcg
    13681 aagtgaaaaa ggtggacgat aacaccgttc agtttgtgct gactcgaccg gaagcgccgt
    13741 tcctcgctga cctggcaatg gacttcgcct ctattctgtc aaaagaatat gctgacgcga
    13801 tgatgaaagc cggtacaccg gaaaaactgg acctcaaccc aatcggaacc ggtccgttcc
    13861 agttacagca gtaccaaaaa gattcccgta tccgctacaa agcgtttgat ggctactggg
    13921 gcaccaaacc gcagatcgat acgctggtct tctctattac ccctgatgct tccgtgcgtt
    13981 acgcgaaatt gcagaagaat gaatgccagg tgatgccgta cccgaacccg gcagacatag
    14041 ctcgcatgaa gcaggataaa tccatcaacc tgatggaaat gccggggctg aacgtcggct
    14101 acctctcgta taacgtgcag aaaaaaccgc tggatgacgt gaaagttcgc caagcactaa
    14161 cctacgcggt gaacaaagac gcgatcatca aagcggttta tcagggcgcg ggtgtatcag
    14221 cgaaaaacct gatcccgcca accatgtggg gctataacga cgacgttcag gattacacct
    14281 acgatcctga aaaagcgaaa gccttgctga aagaagcggg tctggaaaaa ggtttctcca
    14341 tcgacctgtg ggcgatgccg gtacaacgtc cgtataaccc gaacgctcgc cgcatggcgg
    14401 agatgattca ggcagactgg gcgaaagtgg gcgtgcaggc caaaatcgtc acctacgaat
    14461 ggggtgagta cctcaagcgt gcgaaagatg gcgaacatca gacggtaatg atgggctgga
    14521 ctggcgataa cggggacccg gataacttct tcgccaccct gttcagctgc gccgcctctg
    14581 aacaaggttc taactactca aaatggtgct acaaaccgtt tgaagatctg attcaaccgg
    14641 cgcgtgctac cgacgaccat aacaaacgcg ttgaactgta caagcaagcg caggtggtga
    14701 tgcacgatca ggctccggca ctgatcatcg ctcactccac cgtgtttgaa ccggtacgca
    14761 aagaagtcaa aggctatgtg gttgatccat taggcaaaca tcacttcgaa aacgtctcta
    14821 tcgaataatt aaaagccata caagactgat ggcaaaggca aaactgcctg atgtgctccg
    14881 cttatcaggc ctacgaaaat tctgcaatgt attgaatttg cacgattttg taggccggat
    14941 aaggcgttaa cgccgcatcc ggcataaaca aagcgcactt tgtcaacaat ctgtataccc
    15001 ggtggcgctg tgcctctgcg tgcagcgcca tccggcagca atacttcatt ctctgtccga
    15061 caagggcggg aattgatttg tgagcaatac agacacgcag ttccaggctg cgagtcacta
    15121 tagagaatcc gggttatgtt gcagtttatt ctccgacgtt tgggactcgt catccccacg
    15181 tttatcggta ttacccttct cacatttgcc tttgtccaca tgatcccggg cgatccggtg
    15241 atgatcatgg caggcgaacg tgggatctcc cctgagcgtc acgtgcagct gctggctgaa
    15301 ctcggcttag ataaaccgat gtggcagcag tatctccatt acatttgggg cgtaatgcac
    15361 ggcgatctag gcatttcaat gaaaagccga attccagtat gggaagagtt cgtgccgcgc
    15421 tttcaggcca cgctggaact tggcgtctgc gcgatgattt ttgctacggc agtcggtatt
    15481 ccggttggtg tgctggctgc ggttaaacgc ggttccattt tcgatcacac tgcagttggc
    15541 ctggcgctga ccggctactc gatgccgatc ttctggtggg gcatgatgct gatcatgctg
    15601 gtttcggtgc actggaacct gacgcccgtc tccggtcgcg tgagcgatat ggtgttcctc
    15661 gatgactcca atccgttaac cggttttatg ctaatcgaca ccgccatctg gggtgaagac
    15721 ggcaacttta tcgatgccgt cgcccatatg atcttgcccg ccattgtgct gggtactatt
    15781 ccgctagcgg tcattgtgcg tatgacacgc tcctcgatgc tggaagtgct gggcgaggat
    15841 tacatccgca ccgcgcgcgc caaagggcta acccgcatgc gggtgattat cgtccatgcg
    15901 ctgcgtaacg cgatgctgcc ggtagtgacc gttatcggcc tccaggtggg aacattgctg
    15961 gcgggggcga ttctgaccga aaccatcttc tcgtggcccg gtctggggcg ctggttgatt
    16021 gacgcactgc aacgccgcga ctatccggta gtgcagggcg gcgtattgct ggtggcgacg
    16081 atgattatcc tcgtcaactt gctggtcgat ctgctgtacg gcgtggtgaa cccgcgtatt
    16141 cgtcataaga agtaagggga catcatgtca caggttactg aaaataaagt gattagcgca
    16201 ccggtgccga tgaccccgtt acaggagttc tggcactatt ttaaacgcaa caaaggcgcg
    16261 gtcgtcgggc tggtttacgt cgccatcgtg ctgttcatcg cgatctttgc caactggatt
    16321 gcgccttata acccggcgga tcagttccgc gatgcgctgc tcgccccgcc agcctggcag
    16381 gaaggcggca gtatggcaca cttgctgggc accgatgacg tcggtcgtga tgtgctgtcg
    16441 cggctgatgt acggcgcgcg tttatcgctg ctggttggct gtctggtggt ggtgttgtcg
    16501 ctgattatgg gcgttattct cggcctgatc gccggttact tcggcggcct ggttgataac
    16561 attatcatgc gcgtggtcga tatcatgctg gcgctgccaa gtctgctgtt ggcactggtg
    16621 ctggtggcga ttttcggccc gtcgattggt aacgccgcgc tggcgctgac cttcgttgcc
    16681 ttgccgcact atgtgcgctt aacccgcgcc gccgtgctgg tggaggtgaa tcgcgattac
    16741 gtcaccgcct ctcgcgtggc aggtgccggg gcgatgcgcc agatgtttat taacatcttc
    16801 ccgaactgcc ttgcgccgct gattgttcag gcgtcgctcg gtttctctaa cgccattctc
    16861 gatatggctg ctcttggttt cctcggcatg ggggcacagc cgccaacgcc tgagtggggc
    16921 accatgctct ccgacgtgtt gcagttcgcg caaagcgcct ggtgggtcgt gaccttccct
    16981 ggtctggcga tcctgttgac ggtgctggca tttaacctga tgggtgacgg tctgcgtgac
    17041 gcgctcgatc ccaaactaaa gcagtaagag gttcgagatg gcgttattaa atgtagataa
    17101 attatcggtg catttcggcg acgaaagcgc accgttccgc gccgtagacc gcatcagcta
    17161 cagcgtaaaa cagggcgaag tggtcgggat tgtgggtgag tccggctccg gtaagtcggt
    17221 cagttcactg gcgattatgg ggctgattga ttatccgggc cgcgtaatgg cagaaaaact
    17281 ggagtttaac ggccaggatt tgcagcgtat ctcagaaaaa gagcgccgca acctggtggg
    17341 tgccgaagtg gcgatgatct tccaggaccc gatgaccagc cttaacccgt gctacaccgt
    17401 gggtttccag attatggaag cgattaaagt gcatcagggc ggcaacaaaa gtacccgccg
    17461 tcagcgggcg attgacctgc tgaatcaggt cggtattccc gatccggctt cgcgtctgga
    17521 tgtttacccg catcagcttt ccggcggcat gagccagcgc gtgatgatcg ccatggcgat
    17581 tgcctgtcgg ccaaaactgc tgattgccga tgaaccgacc accgcgctgg acgtgaccat
    17641 tcaggcgcaa atcatcgaac tactgctgga gctacagcag aaagagaaca tggcgctggt
    17701 gttaattacc catgacctgg cgctggtggc ggaagctgcg cataaaatca ttgtgatgta
    17761 tgcaggtcag gtagtagaaa cgggcgatgc acacgccatc ttccatgcgc cgcgtcaccc
    17821 gtatactcag gcattgctgc gtgcgctgcc agaatttgct caggacaaag aacgtctggc
    17881 gtcgttgcca ggtgttgttc ccggcaagta tgaccgcccg aacggctgcc tgcttaaccc
    17941 gcgctgcccc tatgccactg acagatgtcg cgctgaagaa ccggcgctga atatgctcgc
    18001 tgatgggcgt cagtccaaat gccattaccc acttgatgat gccgggaggc ccacactatg
    18061 agtacgcaag aggccacctc gcaacaaccg ctgttgcagg ctatcgacct gaaaaaacat
    18121 tatccggtga agaaaggcat gttcgtgccg gaacgtctgg tgaaggcgct ggacggcgtt
    18181 tcgtttaacc ttgaacgtgg caaaacgctg gcagtagtgg gcgaatctgg ctgcggtaaa
    18241 tcgaccctcg gtcggttgct gacgatgatc gaaacgccca ccggcggcga gctgtattat
    18301 caggggcagg atctgctcaa gcacgatccg caggcgcaga agctgcgtcg gcagaaaatc
    18361 cagatcgtct tccagaatcc ctacggttcg ctgaatccgc gtaagaaagt tgggcaaatt
    18421 ctcgaagagc cgctgcttat taacaccagc ttaagcaaag aacagcgtcg ggaaaaagcc
    18481 ctgtcgatga tggcaaaagt cggcctgaaa accgagcact atgaccgcta tccgcacatg
    18541 ttttccggcg gacaacgtca gcgtatcgct attgcccgtg gtctgatgct cgacccggat
    18601 gtggtgattg ccgatgagcc ggtttctgcg ctggacgtct cagtgcgtgc gcaggtgctg
    18661 aatctgatga tggatttgca gcaggagttg gggctttctt atgtctttat ctcccacgac
    18721 ctgtcggttg tggagcacat tgctgatgaa gtgatggtga tgtacctagg ccgctgcgtg
    18781 gagaaaggaa cgaaggacca aatcttcaat aacccgcgcc atccgtacac ccaggcgctg
    18841 ctctccgcga cgccacgcct gaacccggac gatcgtcgtg aacgcatcaa actcaccggt
    18901 gaactgccaa gcccgctgaa tcctccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg
    18961 cgcttcggcc cctgcaccca gttgcagccg cagctaaaag actacggagg tcaactggta
    19021 gcttgttttg ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca
    19081 tgcgctccgg tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtaa
    19141 agcagaatca gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc
    19201 aggaaaaacg gaatgaggca cgacacaatg ccatataacg ggcttcccaa ctgaaagaac
    19261 accagcactg atacacgaaa cgaaacccaa atcgtcaacg taatgacgat aaaagcgcag
    19321 atcgccagag tcagcatgcg tgagttaatt ttcttggtat caataattcg gcttaacaga
    19381 ttgagaataa tgcctttaat agcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc
    19441 agtacggcaa agatattcag caccgtagag gtgatatgaa tgatatgtcc agggatcacc
    19501 tgcgcggcca gtgccagtgc cgagatattt tgttcaaagg cagaaacggc ttcttcgtgg
    19561 ctaattgaga aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa
    19621 ctaattcggt gggtacgcag cgcgagccgg gttgccagta ctttatctgc ttcccgttta
    19681 cgataggcaa tattcattgg gttaagtacc tgaataaata ccgcagaaaa gaagcaaaat
    19741 ggaatggtaa gcaggacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg
    19801 gcgaaattcc agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc
    19861 accaccattg gcccagaaat cttaaacagt aatcgttcac caccagacgc aatcgctatc
    19921 agtacggcga aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa
    19981 ccgaaggttt tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc
    20041 ccgtggataa tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg
    20101 taatgactga taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa
    20161 taaatgtcct gcactaccca ggtgacagga taagcaatga tcgctgcggt aataaatacc
    20221 caaattccct tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg
    20281 cctatgcata ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt
    20341 ttcgatagtg tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat
    20401 gaataaaccc agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa
    20461 atgaggcaac agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat
    20521 ttcactacca gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt
    20581 aagctggaag gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagcaatcg
    20641 tagaatcagc gtcaagccgg aaggattccg gtgaggcgca atgttgcggg ggcttaatcc
    20701 ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gtacctgaca
    20761 acataacggg ggctaatctt gaacctagac cactcaagaa tagccgcgaa acgttgtcat
    20821 tacaacacag gcggctatat gacgctcgca ga
//
LOCUS       DAJTYH010000069        20684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTYH010000069 DAJTYH010000000
VERSION     DAJTYH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            83..352
                     /locus_tag="OO609_002575"
     CDS             83..352
                     /locus_tag="OO609_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6633946.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            346..711
                     /locus_tag="OO609_002576"
     CDS             346..711
                     /locus_tag="OO609_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6633947.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OO609_002577"
     CDS             complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OO609_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6633948.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1538..2221
                     /gene="gntX"
                     /locus_tag="OO609_002578"
     CDS             1538..2221
                     /gene="gntX"
                     /locus_tag="OO609_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6633949.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2280..2855
                     /gene="nfuA"
                     /locus_tag="OO609_002579"
     CDS             2280..2855
                     /gene="nfuA"
                     /locus_tag="OO609_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6633950.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3216..4532
                     /gene="gntT"
                     /locus_tag="OO609_002580"
     CDS             3216..4532
                     /gene="gntT"
                     /locus_tag="OO609_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6633951.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OO609_002581"
     CDS             complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OO609_002581"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6633952.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OO609_002582"
     CDS             complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OO609_002582"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6633953.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9676..12381
                     /gene="malT"
                     /locus_tag="OO609_002583"
                     /pseudo
     CDS             9676..12381
                     /gene="malT"
                     /locus_tag="OO609_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="OO609_002584"
     CDS             complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="OO609_002584"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6633954.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="OO609_002585"
     CDS             complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="OO609_002585"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6633955.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14851..16449
                     /gene="rtcR"
                     /locus_tag="OO609_002586"
     CDS             14851..16449
                     /gene="rtcR"
                     /locus_tag="OO609_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6633956.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16431..17189)
                     /locus_tag="OO609_002587"
     CDS             complement(16431..17189)
                     /locus_tag="OO609_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6633957.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="OO609_002588"
     CDS             complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="OO609_002588"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6633958.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="OO609_002589"
     CDS             complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="OO609_002589"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6633959.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18597..20102
                     /gene="glpD"
                     /locus_tag="OO609_002590"
     CDS             18597..20102
                     /gene="glpD"
                     /locus_tag="OO609_002590"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6633960.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20371..20601)
                     /locus_tag="OO609_002591"
                     /pseudo
     CDS             complement(20371..20601)
                     /locus_tag="OO609_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4889 a         5690 c         5216 g         4889 t
ORIGIN      
        1 gagtcagtag atactctacc ctgcttaagt gctactttgt gctatcttgc atgtaacaca
       61 aagtagcact tagggaactg atatgcagac agtgaaatta cggcaacaag gaggagccat
      121 gatcgttact atcccacgag atcttgccat cgatcttggc tggtcaccgg gcacagagct
      181 taccgtggag aaaaaaggcg atagcgttaa tttgcgcgcc acagagcaca aaccgcgtgg
      241 gcgtcttacc gtcgcgcagt tgttgagcca aatcgatgaa agcgaaatta cggagctaaa
      301 tcagtccaca gaaggatggg ctgaagggaa aaaaggtaat gaagcatggt aaaggcacgg
      361 acgccacatc gtggtgagat ctggtatttt aaccctgatc cggttgccgg gcatgaactt
      421 caggggccac attattgcat tgtggtaacg gacaaaaaac tcaacaatgt tttaaaagtt
      481 gctatgtgct gcccgatttc aacaggggca aatgcagcac gttccacagg ggtgacggtg
      541 aacgtcctcc cccgtgatac gcaaaccggt aacctgcatg gcgttgtact ttgtcaccag
      601 ctaaaagccg tcgatcttat tgcccgtggc gctaaatttc ataccgttgc cgatgaaaaa
      661 ttgattagtg aagttatcag taaactggtg aatttaatcg acccacaata attagctctc
      721 acttaccacc aacaccctct gcttcaacgc caccagcagg tgacaaaact cgaccggatg
      781 cgaaataaac ggcgcatggg ccgctttggc gaagatatat gattcgctgt gaggccaaag
      841 tttatccagc atcggcacca ctttgcgcgg caccagaccg tcgagatagc catacaatcg
      901 caaaaacggc atggacacgt tttgcagcgg ctgacggaga tcgaccgttt tcaggatttc
      961 cagcccgcca ttaagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa
     1021 cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt tgtaacgcca ggaaccgctc
     1081 cactgtacgc tgaaaatcat cactgagttg ctgctgaaat cccgccagca cgtccggttt
     1141 tatccccggc cactcgtcac gagcactaaa acaaggtgac gacgccacgg tgaccagcgc
     1201 ctgaacacgc tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca
     1261 gcctaaccaa atggctttat caggtgcctg ttgcagcacg gcttcggcca tatcagcaag
     1321 tgacagcgca ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa ggtgcagcgt
     1381 aaaatgcgag ctaagttcct cgtcaatgca acgccacact tcggcattca gtccccatcc
     1441 gtgcagcagc acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat
     1501 ccgctattgt tctcttttga cttacaagga tgaacatatg ctaacagtac cgggattatg
     1561 ctggctatgc cgaatgccac tggcgttagg tcattggggg atttgttcgg tctgttcacg
     1621 cgccacccgc acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactccca
     1681 tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg tcacggttgc
     1741 cgactatgcg ccgccgttaa gtccgcttat ccaccagctt aaattttccc ggcgcagtga
     1801 aatcgccagc gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac
     1861 cgggttgcaa ttgccggatc gcatcatcag cgttccgtta tggcagcggc gtcactggcg
     1921 tcggggattt aatcagagcg atttgctgtg tcagccgtta tcacgctggt tgcactgcca
     1981 atgggatagc gaagccgtca cacgtacacg ggccactgcg acccagcatt ttctcagtgc
     2041 ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt gaattgcccg tgcaaggtcg
     2101 ccatatggtg attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca
     2161 gttgctttta cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta
     2221 gagcctcgat gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta
     2281 tgatccgtat ttccgatgct gcacaagcgc actttgccaa actgctggca aatcaggaag
     2341 aagggacaca aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggtg
     2401 tttcttattg tccgccggat gccgtggaag ccaccgacac tgccctgaaa tttgacctgc
     2461 tgaccgcgta tgttgatgag ttaagcgcac catacctgga agatgcagag atcgactttg
     2521 ttaccgacca gttgggttct cagttaacgc tgaaagcccc gaacgccaaa atgcgtaaag
     2581 tggcagacga tgcaccgctg atggagcgcg tggagtatat gctgcagtcg cagatcaacc
     2641 cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa gacggttacg
     2701 ccattctgca atttggcggc ggctgtaacg gttgttccat ggtcgatgtg acgctgaaag
     2761 aagggatcga gaagcagctg ctgaacgaat tcccggagct gaaaggtgtg cgcgatctca
     2821 ccgaacacca gcgcggcgaa cactcctact actaagttat cccctctttt gtagattgcc
     2881 cgatgcgacg ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga cgttggcaca
     2941 gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat aatatgacca acctctcata
     3001 atttaaattt accccgctct ggtgattctc aaacgccaaa tgttacccgt atcattcaca
     3061 tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg ggtatcagtc
     3121 gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt cagaaaattg
     3181 acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt gttgctatcg
     3241 gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc atcgctctcg
     3301 tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa gttattggct
     3361 ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg ggttttggcg
     3421 caatgctggg caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc accacgctga
     3481 ttgccaaatt tggtaaaaag cacatccagt gggcggtggt gttaaccggt tttaccgttg
     3541 gttttgccct gttctatgaa gtgggcttcg tgctgatgct gccgctggtg tttaccatcg
     3601 cggcttccgc gaatattcca ttgctgtatg ttggtgtacc aatggcggct gcactgtctg
     3661 tgacccacgg cttcctgcca ccgcatccgg gcccgactgc gattgccacc attttcaatg
     3721 ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc gtgattctcg
     3781 ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaatcccg gaaggtctct
     3841 acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc gtctggacct
     3901 ctctggtgcc ggttgtgctg atggcgatgc gtgcgattgc cgaaatgatc ctgccgaaag
     3961 gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggca acgctgattg
     4021 ccgtgctgat tgcgatgttc acctttggtc tgaaccgtgg tcgttcaatg gatcagatta
     4081 acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc atcggtggtg
     4141 gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt gcttccatga
     4201 tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc gtactgcgta
     4261 tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg gcaccgctga
     4321 ttgcaacgac gggtgttagc ccagagctga tggttattgc ggtgggttcc ggtagtgtga
     4381 ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt aacctgacta
     4441 tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg tgcggtctgg
     4501 taggctgtct gctgctgaat atggtgattt gataacactg actgccggat gcatcgcatc
     4561 cgacatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct
     4621 tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg gaaagcttgc
     4681 gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca
     4741 gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca
     4801 aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg
     4861 gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct
     4921 gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta
     4981 gatccccgct ctcccagtaa ccacgcagcg ttggcaggtc atgtgtcgcc gcaaccgcca
     5041 tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg ttttcgaaat
     5101 agagcacttt gtaagagtac acaccgctgc tgcgcagctt accaacaatc tctaccggta
     5161 cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa
     5221 tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat
     5281 acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt
     5341 tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg gtgatgatat
     5401 gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata tccggcggcg
     5461 cgccaaccga tgctttcagg caatatagtt cacggtcaca ccaggtttcc gccccacctt
     5521 ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta
     5581 tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga taaaaatcga
     5641 cgtcatcacg atgttcttcg cagaactgac gcacttctgg tgaatccacg ttcttataca
     5701 tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta
     5761 gcgcatcaaa ggcagcctgc cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa
     5821 acgcggccat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg
     5881 tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcgcgc gcctgttgca
     5941 gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat
     6001 cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa gacgggctgt
     6061 atgggctggc gctctccgga tttgccggat agagcgcatg aatcgggttc aggccaatga
     6121 acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa
     6181 taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc cacagctttt
     6241 gtttattcag caaagcctgc ggttcgtaac aacgtttcgg ggcgacaatc acccggcaat
     6301 gcgcgcgctg gtcgtcctgg gtgagtgtta gcgtgtgata accttccggc agcttcgtcg
     6361 gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgcgttcct tcttcggtgg
     6421 tcagctgcca gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg
     6481 tataaaccat gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca
     6541 tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat
     6601 tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca
     6661 tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat
     6721 ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct
     6781 ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg aaatccgcca
     6841 tcaccagata cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat
     6901 gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct
     6961 tatctttctt ccgccatttc accgggtcgt agcctttggc cagaatagcc ttcacttgtt
     7021 ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt
     7081 tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg
     7141 taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca
     7201 gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa
     7261 ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata atattcttcg
     7321 ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag
     7381 cctgcaggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct
     7441 ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct
     7501 caataccggt acgaactttc acaaactccg ccagacagac tttattcgcc tgcttgatct
     7561 cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat
     7621 cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc agtgcagggt
     7681 tgcactgttt gatccagcga cgtggggtaa taccgttggt aacgttatgg aatttgttcg
     7741 gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg
     7801 caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttat
     7861 cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacca
     7921 gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc agtaagcctt
     7981 tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag
     8041 cgaaagtttt gctggtgatg gcccaggcgt catcccagct catctggtgc tcatcggtca
     8101 gcacgcgcag cagttctgga atcgcgatag ttggatgggt atcgttcagc tgaataactt
     8161 cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc aaaatatccg
     8221 ctaccgaaca ggcacactgg aagtattgct gcatcaggcg cagcttttta ccggcagtat
     8281 ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgcct tgctgttcgg
     8341 cgcgcaggaa atcaccgtcg ttaaatttag ttagatcaaa cggatgcgca tgcgtcgcct
     8401 gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg
     8461 cttgaccggt aatggtaaac tccggctccc agcgtccgtc tttcatcact ttaccgccaa
     8521 tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc
     8581 agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac aaaccatatt
     8641 gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag tcgaggaagc
     8701 acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct tcttccagca
     8761 ggtcagtcag gttgatgtca taagccttca acgaatcctg tacatcctga taccagccga
     8821 gattcaacag gttgttgccc gtcagacgac caatcaaaaa ctccattgag atgtagttaa
     8881 catgtcgctg attcgccacc ggtttggcga atggctgagc acgcagcatt tcggccagtg
     8941 cttcactcac tgccagccac cactggcgag gagtcatttc agccgcagaa tttaagccat
     9001 aacgctgcca ctgacgtgaa agcacttcct gaaattgctt atcgttaaaa ataggttgtg
     9061 acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct
     9121 cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca
     9181 aggaaatgtg atctcgacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa
     9241 tgccatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa
     9301 aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata
     9361 tccagtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt agatggcact
     9421 ttcatcagga atgaagaaga aacccttgct taaatgaatc tgatgaacat aagggaaacc
     9481 agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaagatt tggaattgtg
     9541 acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa aggtttctgg
     9601 ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc attttccaca
     9661 gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg actcgaccat
     9721 accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt ccggctggcg
     9781 ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg ggcggcaggc
     9841 aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga gcgtttcgcc
     9901 agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat atgtgagacg
     9961 atggagcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct tttcattgag
    10021 ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct gatcactaat
    10081 ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa tctcaccctg
    10141 gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt tcgtgaccaa
    10201 ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa gcagtttttt
    10261 gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg cgatgacgtt
    10321 tccggttggg cgacggcact atagctaatc gccctctccg cccggcagaa tacccactca
    10381 gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc ggattatctg
    10441 gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt gaaaagcgcc
    10501 attttgcgct caatgaacga tgccctcatc acccgtgtga ccggcgaaga aaacgggcaa
    10561 atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga tgataccggc
    10621 gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg ccagtgggaa
    10681 ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga
    10741 tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat
    10801 attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct gctggaagag
    10861 tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt gttattgcag
    10921 gcgtggctaa tgcaaagcca acatcgctat ggcgaagtta acaccctgct agcccgtgct
    10981 gaacatgaaa tcaaggacat cagagaaggc accatgcatg cagaatttaa cgctctgcgc
    11041 gcccaggtgg cgattaacga tggtaatcag gatgaagcgg aacggctggc aaaactggca
    11101 ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc ggtgctgggt
    11161 gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctagtgca gcaaaccgaa
    11221 cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat tcagcaaagt
    11281 gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacgcagga aaaagcgttc
    11341 cagctgatca acgagcagca tctggaacag ctgccaatgc atgagtttct ggtgcgcatc
    11401 cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc ggcgcgtagc
    11461 gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct ggcaatgttg
    11521 attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagtt gaaccgtctg
    11581 gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc caacaaagtc
    11641 cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg gttgcgtcat
    11701 acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg caacattgcc
    11761 cgcgcacaaa tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga agaactcaat
    11821 gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt gctgcttaat
    11881 caactgtact ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct ggacgcatta
    11941 aaactggcga atcgcaccgg atttatcagt cattttgtca tcgaaggcga agcgatggcg
    12001 caacaactgc gccagctgat tcaacttaat acgctgccgg aactggaaca gcatcgcgcg
    12061 cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca tttcgatgag
    12121 aatttcgttg aacgtctgct aaatcatccg gaagtgccag aactaatccg caccagcccg
    12181 ttgacccaac gtgaatggca ggtcctgggg ctgatctact ccggttacag caatgaacaa
    12241 attgcgggcg aactggaagt ggcggcaacc accatcaaga cgcatatccg caatctgtac
    12301 caaaaactcg gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa actgctgaag
    12361 atgatgggtt atggggtgtg agtttagccg gataatgcat tggatccggt ttacatcttt
    12421 acatcattca atactcaccc gcgttacgcc atctgtttct atcaaaccaa accgcaccgg
    12481 caagaaacgc tccaccaccg cgatattggt cagcagatgg catgaaggat gggcgaccgt
    12541 aaattctccc gcgcccgcca gcgccatcgg cagtaccagt tggtcggcga gatattcccc
    12601 caccgccgct gggcttgcca ggtagcgttt cacctctttc actaactgcg cggctaccac
    12661 ctcggcactg acgcgctttt caccgacgac gaaaaagcgt tcagtgatat tttcactttc
    12721 gacttccagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat gaatattctg
    12781 ttcatgcagg gaaaaaatcc ccgccagtgt agcgatttca cgctcagcaa catggcgcgg
    12841 cacaccagct aatagaactt ctccacgcat ctgcacaatg ttcccgcgct cgccaagttg
    12901 caaggtgcta aacgatgcca cgggcgagac ttccgttgcc accacaccgc ctccggcagg
    12961 ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg ccagcagcgg
    13021 ctccagcacc cggcggataa aatccgcagg cggcgccgac gggttatcgg tgccgccgct
    13081 cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg tttgcagcac
    13141 cagcgtacaa cttccggcgc taccgatagc aaagcggtaa tcgccgccgc gcacggtgcc
    13201 aggccggaag accagacgct gcgatcccag ctccgcgcct tccaccgttg ccctacaaat
    13261 ttccgcagcc gcttttaccg cggtcagatg ctggcgcagc agccccggtt tcgcccgtcc
    13321 ggctcgaatg ccagtgatgg taaatggctg gccggttatc atcgacaggc tcagcgccga
    13381 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
    13441 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
    13501 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
    13561 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
    13621 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
    13681 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
    13741 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
    13801 aaagagctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
    13861 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
    13921 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
    13981 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
    14041 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
    14101 tccgcgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
    14161 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
    14221 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
    14281 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
    14341 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
    14401 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
    14461 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
    14521 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
    14581 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
    14641 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
    14701 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaattta ttgtttctaa
    14761 aaagattata tctttatatc cgtaaacgga gatttcccgc aaagccgatt taccgataat
    14821 gaaatatcgc cttttataag gatatctaag atgcgtaaaa cagtggcttt tggctttgtc
    14881 ggtaccgtgc tggattatgc cggacgcggc agtcagcgct ggtcaaaatg gcgtccgtca
    14941 ctctgtatat accagcaaga atcgttggtc atcgatcgac tggaattatt gcacgatgcc
    15001 cgctcgcgct cgctatttga aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca
    15061 gaagtggtgg gcgttgagat tgaactgcat aacccgtggg atttcgaaga ggtttacgcc
    15121 tgcctgcatg atttcgcccg tggttacgag tttcagccag aaaaagaaga ctatttaatt
    15181 cacatcacca ccggcaccca cgtcgcgcag atttgctggt ttctgctggc ggaagcgcgt
    15241 tatctacccg cccggttgat acaatcttca actccgcgca aaaaagaaca gccgcgcggc
    15301 ccaggtgaag tgacgattat cgatctcgat ttaagccgtt ataacgccat cgccagccgc
    15361 tttgccgagg aacggcagca aacgctcgat ttcctcaaat ccggtattgc cacgcgtaac
    15421 ccccacttca accgcatgat tgagcagatc gaaaaagtgg cgattaaatc ccgcgcgccg
    15481 attctgctta acggcccgac cggcgcaggt aagtcatttc tggcgcgacg catcttcgag
    15541 ttaaaacagg cacggcatca gtttagcggc gcgtttgtgg aagtgaactg cgccaccctg
    15601 cgcggcgata ccgcgatgtc gacgctgttt ggtcatgtga aaggcgcgtt taccggggcg
    15661 cgggaatctc gtgaaggatt attacgcagc gccaacggcg gaatgttgtt tcttgatgag
    15721 attggcgaac tgggcgcaga cgaacaggca atgctgctga aagctattga agagaaaacc
    15781 ttttacccgt ttggcagcga tcgccaggtg agcagtgatt ttcagcttat cgccggaacg
    15841 gtgcgcgatt tgcgccagct ggttgccgaa ggcaaattcc gcgaagatct gtacgcgcgg
    15901 atcaatctct ggaccttcac cctgccgggt ctacgccagc gccaggaaga tattgaaccg
    15961 aacctggatt atgaagtgga gcgccacgcc acactcactg gtgacagcgt gcgttttaac
    16021 accgaagcgc ggcgtgcctg gctggccttt gcaacatctc cccaggcaac atggcgcggt
    16081 aactttcgcg aactttctgc cagcgtcacg cggatggcca cctttgccac tagcggacgc
    16141 atcactctgg acgtggtgga agatgagata aaccgtctgc gctataactg gcaggagagc
    16201 cgcccctccg cgcttacggc gttgctgggc gcagaggcag aaaacatcga tctcttcgac
    16261 cgtatgcaac tggaacacgt tatcgctatc tgccgccagg caaagtcgct ttctgctgcc
    16321 ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca gcgtcaatga cgcggatcgg
    16381 ctacgcaaat acctggcgcg ttttggtctg acgtgggaag ccgtgcagga tcagcacagc
    16441 tccagttgaa tatggtggtc cgtcagcacc tgcatcacgc ttactggcgg cggggcgtcg
    16501 gtgtagacgg catcaaccat gctgatgctc cccatattga ccatcgcatt acggccaaat
    16561 tttgaatggt caaccaccag cataacgtgg cgcgagttct caataatggc gcgcttggta
    16621 cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc tgtcgatgcc gctgatcccc
    16681 agaatgccga aatcaaggcg gaactgggag ataaaatcga gcgtcgcttc gccaatgatc
    16741 ccgccatcgc ggctacgtaa ttcgccaccg gcgagaataa tgcggaaatc ttcttttacc
    16801 atcaacgtgt tagcaacgtt gagattgttg gtgacaatgc gcaaattgct gtgattgagc
    16861 agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagcgt cgagccattg
    16921 gggatttgct ccgccacttt gcgggcgatg cgctcttttt cttcggtctg ggtggcttta
    16981 cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg ccgcgccgcc atgatggcgc
    17041 aggatcaggt tttgctctgc cagctcgttg aggtcgcggc gaatagtctg cgggctgacg
    17101 gagaagtgct ccaccagttc ctcggtactg acataaccct gctgtttaac cagttcgata
    17161 ataccgttgt gacgttgtgt ttgtttcatt tataaatccc tggaattatt ttcgttttcg
    17221 cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaatccgg cgatgtgtgc
    17281 tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc agatcagcgc
    17341 aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt cgccacgtag
    17401 ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc acggcccgct
    17461 gaatttttgc tgcatatagc cgcttaacag ggcgctgata agcgtaatga caattagctt
    17521 accgctaccg aggcgttttt ccaccgcgcc gccgagatac caccaccaaa gcaggttaaa
    17581 gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa
    17641 tttcagtgtt ggatcgaatg gccaggccag ccataacatc acttcctgat cgccgagaat
    17701 ttgcatggca ataaacacca ccacgcaggc gatcatcatc acccaggtca ccggacctgc
    17761 gcgttcacgc aaggcagcaa agaaaggata acggcgataa tgcaggccac tgccggtatg
    17821 gcctgccagc cagctggccg ccagataacg cggatctgcc gggttttcga gaaaacgcgc
    17881 cagctccgcc cgcacgcgct cggcctggga ctcatccgcc agccagacat cgctttggtt
    17941 atgttgttga atcgtgagga taacaccctg cgtcgccatg taatcaacaa acgcctgcgc
    18001 cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt attccacaca
    18061 aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa ctgtcgctgc
    18121 caggcttcaa agccgccgtc aatgctatag accacatcgt agccctgttg cagcagatac
    18181 tgcgccgccc ctttgctgct attgccgtga taacacatca ccatcaccgg agtgtcaaag
    18241 tcgttatcac gcataaacgc gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca
    18301 tgccccatag cgaaactctg tggatcgcga atatcgacca gcaccgcctc tttttcctgc
    18361 aacttctggt gcgcgtcggc aacgttaata cattcgaact gatccatgcg tctctctttc
    18421 tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag ataaacgcca
    18481 taatgttata catatcactc taaaatgttt tttcaatgtt acccaaagcg cgattctttg
    18541 ctaatatgtt cgataacgaa catttatgag ctttaacgaa agtgaatgag ggcagcatgg
    18601 aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg tgctggtatc gcggcagacg
    18661 ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct tgcgcgacct
    18721 cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac tatgaattcc
    18781 gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct gaaaatggct ccgcatatcg
    18841 ccttcccgat gcgttttcgc ctgccacatc gtccgcatct gcgcccggcg tggatgattc
    18901 gcattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg ggatcaactg
    18961 gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat taagcgcgga ttcgaatatt
    19021 ctgactgttg ggtagacgac gcccgtctgg tactcgccaa cgcccagatg gtggtgcgta
    19081 aaggcggcga agtgcttact cggactcgcg ccacctctgc tcgccgcgaa aacggcctgt
    19141 ggattgtgga agcggaagat atcgataccg gcaaaaaata tagctggcaa gcgcgcggcc
    19201 tggttaacgc caccggcccg tgggtgaaac agttcttcga cgaggggatg catctgcctt
    19261 cgccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgc gtgcataccc
    19321 aaaagcaggc ctacattctg caaaacgaag ataaacgcat tgtgttcgtg atcccgtgga
    19381 tggacgagtt ttccatcatc ggcaccaccg atgtggaata caaaggtgat ccgaaagcag
    19441 tgaagattga agagagtgaa atcaattacc tgctgaaagt gtataacgcg cactttaaaa
    19501 agcagctaag ccgtgacgat atcgtctgga cctactccgg tgtgcgtccg ctgtgtgatg
    19561 atgagtccga ctcgccgcaa gccattaccc gtgattacac ccttgatatc catgatgaaa
    19621 acggcaaagc accgctgctg tcggtattcg gcggtaagct gaccacctac cgaaaactgg
    19681 cagaacatgc gctggaaaaa ctgacgccgt attatcaggg tattggcccg gcgtggacga
    19741 aagagagcgt gctgccgggt ggtgccattg aaggcgaccg cgacgattac gccgctcgcc
    19801 tgcgccgccg ttacccgttc ctgactgaat cgctggcacg tcactacacg cgcacttacg
    19861 gcagcaacag cgagctgctg ctcggcaatg cgggaacggt aagcgatctc ggggaagatt
    19921 tcggtcatga gttctacgaa gcggaactga aatacctggt ggatcacgaa tgggtccgcc
    19981 gcaccgacga tgccctgtgg cgtcgcacaa aacaaggcat gtggctgaat gcggatcaac
    20041 aatctcgtgt gagtcagtgg ctggtggaat atacgcagca gaagttgtcg ctggcgtcgt
    20101 aaattaacgt aaggtgatca ggtcagattt caatctggcc tgagactgat gacaaacaca
    20161 aaactgcctg atgcgctacg cttatcaggc ctacgtggtt tatgcaatat attgaatttg
    20221 catggtcttg taggccagat aagacgttca cgtcgcatcc ggcatgaaca aagcgcactt
    20281 tgtcaaaaat ctaacctact ttttaattca ggaaattacc gcaaagccca cgccatcata
    20341 cccaacgtaa caaaactcag gcacgttccc cactcgcccc gagaaaaata gcattaatgc
    20401 gcccagcgtc agcataaaaa tattgagcag aaggtaaatt gttggcgtaa agagtaaatt
    20461 gcaaacctca ggaatagcgg caagcccagc ccccagcatg gcaataaccg ctgcaaaaaa
    20521 ttgcgtcagt attgggatgc cgaaaaaagt cattaccagc gaggtcaaaa tccattttct
    20581 attttgcatt attctttcca ttctttctga atggcgaaat tatactcccc gagtcccctt
    20641 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cggg
//
LOCUS       DAJTYH010000070        20664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTYH010000070 DAJTYH010000000
VERSION     DAJTYH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..1670)
                     /gene="glyS"
                     /locus_tag="OO609_002592"
     CDS             complement(<1..1670)
                     /gene="glyS"
                     /locus_tag="OO609_002592"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6633961.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRP"
     gene            complement(1680..2591)
                     /gene="glyQ"
                     /locus_tag="OO609_002593"
     CDS             complement(1680..2591)
                     /gene="glyQ"
                     /locus_tag="OO609_002593"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6633962.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(2686..2985)
                     /locus_tag="OO609_002594"
     CDS             complement(2686..2985)
                     /locus_tag="OO609_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6633963.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3160..4155
                     /gene="wecH"
                     /locus_tag="OO609_002595"
     CDS             3160..4155
                     /gene="wecH"
                     /locus_tag="OO609_002595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6633964.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4197..4634)
                     /locus_tag="OO609_002596"
     CDS             complement(4197..4634)
                     /locus_tag="OO609_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6633965.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(4680..5021)
                     /locus_tag="OO609_002597"
     CDS             complement(4680..5021)
                     /locus_tag="OO609_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6633966.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5190..6644)
                     /gene="xylB"
                     /locus_tag="OO609_002598"
     CDS             complement(5190..6644)
                     /gene="xylB"
                     /locus_tag="OO609_002598"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6633967.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELVRAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(6716..8038)
                     /gene="xylA"
                     /locus_tag="OO609_002599"
                     /pseudo
     CDS             complement(6716..8038)
                     /gene="xylA"
                     /locus_tag="OO609_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8404..9396
                     /gene="xylF"
                     /locus_tag="OO609_002600"
     CDS             8404..9396
                     /gene="xylF"
                     /locus_tag="OO609_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6633968.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9474..11015
                     /gene="xylG"
                     /locus_tag="OO609_002601"
     CDS             9474..11015
                     /gene="xylG"
                     /locus_tag="OO609_002601"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6633969.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            10993..12174
                     /gene="xylH"
                     /locus_tag="OO609_002602"
     CDS             10993..12174
                     /gene="xylH"
                     /locus_tag="OO609_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6633970.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12252..12782
                     /locus_tag="OO609_002603"
     CDS             12252..12782
                     /locus_tag="OO609_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6633971.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            12894..13430
                     /locus_tag="OO609_002604"
     CDS             12894..13430
                     /locus_tag="OO609_002604"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6633972.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13538..14362)
                     /locus_tag="OO609_002605"
     CDS             complement(13538..14362)
                     /locus_tag="OO609_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6633973.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            14682..16712
                     /gene="malS"
                     /locus_tag="OO609_002606"
     CDS             14682..16712
                     /gene="malS"
                     /locus_tag="OO609_002606"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6633974.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            16890..18143
                     /gene="avtA"
                     /locus_tag="OO609_002607"
     CDS             16890..18143
                     /gene="avtA"
                     /locus_tag="OO609_002607"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6633975.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18294..18767)
                     /locus_tag="OO609_002608"
     CDS             complement(18294..18767)
                     /locus_tag="OO609_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6633976.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(18869..19717)
                     /gene="yiaJ"
                     /locus_tag="OO609_002609"
     CDS             complement(18869..19717)
                     /gene="yiaJ"
                     /locus_tag="OO609_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6633977.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            19917..>20664
                     /gene="yiaK"
                     /locus_tag="OO609_002610"
     CDS             19917..>20664
                     /gene="yiaK"
                     /locus_tag="OO609_002610"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6633978.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEV"
BASE COUNT         5200 a         5245 c         5076 g         5143 t
ORIGIN      
        1 ggacgagtcg gacgacgcgc cagtaccgcc tggatggtgt caacggtata accttcgtcc
       61 tgataccagg cgcggaagcg accgagcata aagtcgataa catcatcaac tacgttggca
      121 ttagtcagct tatcgccata cagacgcacc gcttcttcgg tcagcgtttg cagatcaagg
      181 ttgaggttct tctcaacgat aattcgcagc acgccaagtg cggcacgacg cagcgcaaac
      241 gggtctttgt cgcctttcgg atgctgaccg ataccgaaga tacccgccag agtatccatc
      301 ttatcagcaa tcgccagcgc acaagctacc gggttggacg gcaggtcatc accggcaaag
      361 cgcggctgat actgctcatt cagcgcgacg gctacgtctt ccgcttcgcc atcgtgacgc
      421 gcgtagtgca tccccataac gccttgggtg tcggtgaact cgaagaccat gttggtcatc
      481 aggtcacact tagacagcag gcccgcacgg gttgcgtggt taacgtcagc gccaatctgt
      541 tcggcgatcc agccagccag cgcctggatg cggtcagtct tgtcacgcag cgtacccagc
      601 tgttgctgga acagcacggt ttgcaggcgc ggcaggttat cttccagacg ttttttgcgg
      661 tcggtgttga agaagaactc ggcatccgcc agacgtggac gaacgacttt ctcgttacca
      721 gagataattt gctgcggatc tttcgattcg atgttggcaa cgaagataaa gttcggcagc
      781 agtttgccgt cgttcgcata caccgggaag tacttctggt cacctttcat ggtgtaaacc
      841 agggcttcag ccggcaccgc gaggaatttc tcttcgaatt ttgcggtcag aacgaccggc
      901 cactccacca gcgaagccac ttcttccagc aggctttcgc ttaagtcagc gttaccgcca
      961 atcttacgcg ctgcttcttc ggcatcggct ttaatcttcg ctttacgttc ttcgtaatcg
     1021 gcgatgactt tcccgcgctc acgcagaatt tccggatact gatcggcgtt gtcgatggtg
     1081 aattccggtt cgcccataaa gcggtggccg cgaatcacgc gatcggactg aatgcccaga
     1141 atggttgccg gaatgacttt gtcgcccagc agcagggtca cggtgtgcac cggacgcacg
     1201 aagtgcacgt cgcttgcacc ccagcgcatc agtttcggga tcggcagttt cgccagcgaa
     1261 gtcgcaacca tattcggcag cagtgcttcg gtgctttcgc ccttcacatg ggcgcgatac
     1321 agcagccatt cgcctttatc ggtagtcaga cgctcagcct ggtcaacggt aataccacaa
     1381 ccacgcgccc aaccttctgc cgctttgctc ggttttcctt cagcgtcgaa cgcctgggca
     1441 atcgccgggc cgcgtttttc gatttcacga tctggttgtg cttccgccag gttagctact
     1501 ttcagcgcca gacgacgcgg agcagcaaac cattgaacgg tgccgtgtgc gaggcctgcg
     1561 ttatccagct ccgcagtaaa gttcgcagca aaggactctg ccaggctgcg cagtgctttt
     1621 ggtggcagct cttcagtgcc gatttccacc agaaaagttt tctcagacat agccgcctct
     1681 tacttatctt tgttgcacat cgggaagccg agggcttcac gggaagcgta atatgcttct
     1741 gccactgctt tggtcagggt gcgaatgcgc agaatgtagc gctgacgctc ggtgacggag
     1801 atggctttac gcgcatccag caggttgaag ctgtgggcgg ctttcagaat acgctcgtag
     1861 gctggcagcg gcagcggatt ttccagcgcc agcagctgct gcgcttcttt ctcgtactgc
     1921 tcaaagcagg tgaacaggaa gtccacatcc gcgtattcga agttgtaagt ggactgctcc
     1981 acttcgttct gatggaacac gtcgccgtag gtggttttac ccagcgggcc gtcgctccag
     2041 actaggtcgt aaacgctgtc tacgccctga atgtacatgg ccagacgttc cagaccgtag
     2101 gtgatctcgc cggtaaccgg tttacactcc agaccaccaa cctgctggaa gtaagtgaac
     2161 tgcgtcactt ccatgccgtt tagccacact tcccagccca gtccccaggc ccccagagtc
     2221 gggttttccc agttatcttc cacgaaacgg atgtcgtgaa tagtcgggtc catgcccagc
     2281 tctttcagag aaccgaggta cagctcctga atattgtccg gcgatggctt aatgaccacc
     2341 tggaactgat agtagtgctg taaacggttg gggttttcgc cgtagcgacc atcggtcggg
     2401 cgacgagaag gctgaacata agcagccgcc atcggttctg gccccagcgc gcgcagacag
     2461 gtcattgggt gagaggttcc cgcgccgact tccatgtcca atggttgaac aatggtgcag
     2521 ccctggcgag cccagtaatc ctgtaaggtc aggatcaagc cctggaaggt cctggtatca
     2581 aacttttgca tattatttcg tgctggatac gtgtggattt aaaggaaggg atcagtatac
     2641 ccgctggatg gaagatatac agtacgaaac gggaaaaagc agggcttaac gcatggaaag
     2701 atgcaaaaaa tggccatctg cgtcaaaaga acagacaaac tgctctttac ggaaggtata
     2761 accgcgcatt tcatagcttc cctggaactg ttcgaaggcg gtgacgtcga ttttttgctc
     2821 gccggtgtta taacgttttg ccgcctgatc cttgcataac gcttccatat tcaacgaacg
     2881 cagaggatca acccttaccc gttgtgcttt ctgcacgggt gacggtgtag aacaacccac
     2941 tagcaccata agcgccattg ccggaaagaa agcgttcatc atcattaaat taccgtctgg
     3001 tttgcaggtg agtcttatta tttatatcgg tatagtggct gatatcttac gaatttcctt
     3061 aagcctctat aggctgtagt gataaaactc agattattcc agtgcgccaa ccaaatgatt
     3121 tatcggtcag acttttttta accagataca gagacacaga tgcagcccaa aatttactgg
     3181 attgataacc tgcgagggat agcgtgttta atggtggtga tgattcacac cactacctgg
     3241 tatgtgacca atgctcatag tgttagcccc gtcacatggg atatcgccaa tgttctgaat
     3301 tctgcctctc gtgtcagcgt gccgctattt ttcatgattt ccggctatct cttttttggc
     3361 gaacgcagcg cccagccgcg ccatttcttg cgtatcggct tatgtctgat tttttatagc
     3421 gcaatcgcac tgctctacat tgcgctgttt acctccatca atatggagtt agcgctgaaa
     3481 aacctgctgc aaaagccagt gttttaccac ttgtggtttt tcttcgcgat tgcggtgatt
     3541 tatctggttt caccgctgat tcaggtgaag aacgtcggcg gaaaaatgtt gctggtgcta
     3601 atggtggtga ttggcattat cgctaaccca aacacagtgc cgcagaaaat tgacggtttt
     3661 gaatggctgc caattaactt atatatcaat ggcgatactt tttactacat tctgtatggc
     3721 atgttgggcc gcgctatagg gatgatggac acacagcata aagcactgtc gtgggtgagc
     3781 gccgcgctgt ttgcgacggg ggtttttatt atctctcgcg ggacattata tgaattgcag
     3841 tggcgcggaa attttgccga tacctggtat ctttactgtg ggccgatggt ttttatctgc
     3901 gcaatcgcgc tattgactct ggttaaaaac acgctggata cgcgtaccat tcgcggactt
     3961 ggcttaatct cccgccattc attgggtata tacggattcc acgccttgat tatccatgcg
     4021 ctgcgcaccc ggggaattga gcttaaaaat tggccaatac tggatattat ttggatcttt
     4081 tgcgcgacgt tggcagcgag tttgttactt tctatgctgg tacaacgaat cgacagaaac
     4141 agattagtga gttaagtaaa agcccgttca cattggactg accgggctta cgtgaattat
     4201 tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg ggtttattcc ggcatcacga
     4261 atattcttct gcaccgctac tgcaccaaac aagcttaaga agaaagccag tccataaaaa
     4321 cctttttcac tgagtaataa tgtcgcattc cacagaccta ccatcagtag agcaacagag
     4381 ataataaaga cagtcaggca ggtcatataa taaatggaag tggtcggtat gccttcatac
     4441 ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca gtcctaacac cagtacggca
     4501 aaataatacc ctttttcatt taactgcatc tctgcattcc atagccctaa caggtaggta
     4561 acgataccac caacgagagc tatccatgac acaatactaa aggccggtga ataggttgat
     4621 attttgttgt ccatcacagt attcctttca ttcctgaatg tgtaagagct ttatgttgct
     4681 cataccgagc gaaacaaacg catttgacca aacaagcaga caaaaaaggc gatggggtag
     4741 atcaccattt cataagcatt gataggtgca tttaaaacac cgataaacaa gagaccgata
     4801 gtgattaacg caactaactg gcagatatgg gtaaaaaaat tatccagttg cccgagtttt
     4861 tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca ttaacacgcc aagaaaatag
     4921 ccttttccac ttagcaatgg gcatgagatc catagcccaa ccagataaac cagcgccccg
     4981 acaacaaata atagttttgc cacagttttt gaggttttca taacgatctc catatctacc
     5041 agcgatacat tacgagtaac caacgaaaga caaaactgaa aaatgccatt aacaaatgat
     5101 tttcagaata aattcatact aaatattaat taattagtga gatatataga tgtgaattat
     5161 cccccacccg gtcaggcagg ggataacgtt tacgccatta atggcagaag ttgctgatag
     5221 aggcgacgga acgtttcacg tcgtggctga taagcggcat aacgctgcgc atctggtaga
     5281 tgcgactgtt ctaacggtag ctgcggcaac aactcaatga gcgatttctc tggattcgcc
     5341 gcaatctgcg ccagccttgc tgcgcccagt gctggcccca catccccccc cgtacggtaa
     5401 tcgagctgct gaccgctgat atccgccagc atctgacgcc agtactcact acgcgccccg
     5461 ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac atccatgcca
     5521 tctgccagcg cataacccac gccttccagc actgctcgca ccagttcatt ggggccatgt
     5581 tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg tgtacgctcg
     5641 ccggaaagat aaggcagaaa ccaaactggc tcggcacttt catcagcctg ttgagctgca
     5701 gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc cagacacgat
     5761 gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg gcaaaagctg
     5821 tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata gacccccgac
     5881 gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac tgcaccagct
     5941 gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc tttcgcaact
     6001 tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat ctggtcacga
     6061 gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac atccagccac
     6121 atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg cagacgcaag
     6181 taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc atgccgctga
     6241 acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt aatcactcgt
     6301 gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc gtcgttccac
     6361 aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc atgcatctgg
     6421 ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag agctttcatt
     6481 gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccaaag tggatgcggg
     6541 cgcgaaacgg tcagcttttc cgtttgcgaa gcaaccacct caccctgctc gttgagcaaa
     6601 ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat cgttccttaa
     6661 aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt agccgttatt
     6721 tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca ctctgatgca
     6781 ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac atttggcctt
     6841 tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg atgcgtttat
     6901 ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc atcgtatcca
     6961 tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga cgtactttgg
     7021 catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca tacatcacca
     7081 gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc
     7141 cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct atttcatgat
     7201 ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta atctcttttt
     7261 ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca tattgatgtt
     7321 tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctagaagccg attttatgtt
     7381 tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga cgcaagtcgg
     7441 tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt tcaccgccca
     7501 atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg aagacttcag
     7561 gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag ttagcggttc
     7621 cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca accatttgcg
     7681 caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc acatcgtgga
     7741 agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct gctttacgct
     7801 tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc cccacaccaa
     7861 acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct
     7921 cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat gctaacgggt
     7981 ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag gcttgcatat
     8041 tgaactccat aatcaggtaa tgccgcgggt gatggatgat gtcgtaatat tgggcactcc
     8101 ctttcaattg ctcaattatg ttatttcaca ctgctattga gataattcac aagtgtgcgc
     8161 tcgctcgcaa aataaaatgg gatgatgaaa atgggtaatt cctcgaagat aaaaatgcaa
     8221 taagtacaat tgcgcaacaa aagtaagatc tcggtcataa atcaagaaat aaaccaaaaa
     8281 tcgtaatcga aagataaaaa tctgtaattg ttttcccctg tttagttgct aaaaattggt
     8341 tacgtttatc gcggtgattg ttacttatta aagctgtcct ctaactacag aaggccctac
     8401 accatgaaaa taaagaacat tctactcacc ctttgcacct cacttctgct taccaacgtt
     8461 gcggcacacg ccaaagaagt caaaataggt atggcgattg atgatctccg tcttgaacgc
     8521 tggcaaaaag atcgagatat ttttgtgaaa aaggcagaat ctctcggtgc gaaagtattt
     8581 gtacagtctg caaatggcaa tgaagaaaca caaatgtcgc agattgaaaa catgattaac
     8641 cggggtgtcg atgttcttgt cattattccg tataacggtc aggtattaag taacgttgta
     8701 aaaaaagcca aacaagaagg tattaaagta ttagcttacg accgtatgat taacgatgcg
     8761 gatatcgatt tttatatttc tttcgataac gaaaaagtcg gtgaactgca ggcaaaagcc
     8821 ttggtcgata ttgttccgca aggtaattac ttcctgatgg gcggctcgcc ggtagataac
     8881 aacgccaagc tgttccgcgc cggacaaatg aaagtgttaa aaccttacgt tgattctgga
     8941 aaaattaaag tcgttggtga ccaatgggtt gatggctggt taccggaaaa cgcattgaaa
     9001 attatggaaa acgcgctaac cgccaataat aacaaaattg atgctgtagt tgcctcaaac
     9061 gatgccaccg caggtggggc aattcaggca ttaagcgcgc aaggtttatc agggaaagta
     9121 gcaatttccg gccaggatgc ggatctcgca ggtattaaac gtattgctgc cggtacgcaa
     9181 actatgacgg tgtataaacc tattacattg ctggcaaata ctgccgcaga aattgccgtt
     9241 gaattgggca atggtcagga gccaaaagca gatacctcac tgaataatgg cctgaaagat
     9301 gtcccctccc gcctcctgac accgatcgat gtgaataaaa acaacatcaa agatacggta
     9361 attaaagacg gattccacaa agagagcgag ctgtaagcgt tacgccccgg cgcggagcgg
     9421 gggcgtgatt tctctccatg ccgcgtgaat gaattggctt aggtggagtc gttatgcctt
     9481 atctacttga aatgaagaac attaccaaaa ccttcggcag tgtgaaggcg attgataacg
     9541 tcagcttgcg gttgaatgct ggcgaaatcg tctcactctg tggggaaaat gggtctggta
     9601 aatcaacgct gatgaaagtg ctgtgtggta tttatcccca tggctcctac gaaggcgaaa
     9661 ttatttttgc gggagaagag attcaggcga gtcacatccg cgataccgaa cgcaaaggta
     9721 tcgccatcat tcaccaggaa ttggccctgg tgaaagaatt gaccgtgctg gaaaatatct
     9781 tcctgggtaa cgaaataacc cacaatggca ttatggatta tgacctgatg acgctacgct
     9841 atcagaagct gctcgcacag gtcagtttat ccatttcacc tgatacccgc gttggcgatt
     9901 tagggcttgg gcaacaacaa ctggttgaaa ttgccaaggc acttaataaa caggtgcgct
     9961 tgttaattct cgatgaaccg acagcctcat taactgagca ggaaacgtcg gttttactgg
    10021 atattattcg cgatctacaa cagcacggta tcgcctgtat ttatatttcg cacaaactca
    10081 acgaagtcaa agcgatttcc gatacgattt gcgttattcg cgacggacag cacattggta
    10141 cgcgtgatgc tgccggaatg agtgaagacg atattatcac catgatggtc gagcgagagt
    10201 taaccgcgat ttaccctaat gaaccacata ccaccggaga tgaaatatta cgtattgaac
    10261 atctgacggc atggcatccg gtcaatcgtc atattaaacg agttaatgat gtctcgtttt
    10321 ccctgaaacg tggcgaaata ctgggtattg ccggactcgt tggtgccgga cgtaccgaga
    10381 ccattcagtg cctgtttggc gtgtggcccg gacaatggga aggaaaaatt tatattgatg
    10441 gcaaacaggt agatattcgt aactgtcagc aagccatcac ccaggggatt gcgatggtac
    10501 ccgaagacag aaagcgcgac ggcatcgttc cggtaatggc ggttggtaaa aatattaccc
    10561 tcgccgcact caataaattt accggtggca ttagccagct tgatgacgcg gcagagcaaa
    10621 aatgtattct ggaatcaatt cagcaactca aagttaaaac gtcgtccccc gaccttgcta
    10681 ttggacgttt gagcggcggc aatcagcaaa aagcgatcct cgcccgctgt ctgttactta
    10741 acccgcgcat cctcattctt gatgaaccta ccaggggtat tgatattggc gcgaaatatg
    10801 agatctacaa attaattaac caactcgtcc agcagggtat tgccgttatt gtcatctctt
    10861 ccgaattacc tgaagtgctc ggccttagcg atcgtgtact ggtgatgcat gaagggaaac
    10921 taaaagccaa cctgataaat cataacctga ctcaggagca ggtgatggaa gccgcattga
    10981 ggagcgaaca tcatgtcgaa aagcaatccg tctgaagtga aattggccgt accgacatcc
    11041 ggtggctttt ctgggctgaa atcactgaat ttgcaggtct tcgtgatgat tgcagctatc
    11101 atcgcaatca tgctgttctt tacctggacc accgatggtg cctacttaag cgcccgtaac
    11161 gtctccaacc tgttacgcca gaccgcgatt accggcatcc tcgcggtagg aatggtgttc
    11221 gtcataattt ctgctgaaat cgacctttcc gtcggctcaa tgatggggct attaggtggc
    11281 gtcgcggcga tttgtgacgt ctggttaggc tggcctttgc cacttaccat cattgtgacg
    11341 ttggttctgg gactgcttct cggtgcctgg aacggatggt gggtcgcgta ccgcaaagtc
    11401 ccttcattta ttgtcaccct cgcgggcatg ttggcatttc gcggcatact cattggcatc
    11461 accaacggca cgactgtatc ccccaccagc gccgcgatgt cacaaattgg gcaaagctat
    11521 ctccccgcca gtaccggctt catcattggc gcgcttggct taatggcttt tgttggttgg
    11581 caatggcgcg gaagaatgcg ccgtcaggct ttgggtttac agtctccggc ctctaccgca
    11641 gtagtcggtc gccaagcttt aaccgctatc atcgtattag gcgcaatctg gctgttgaat
    11701 gattaccgtg gcgttcccac tcctgttctg ctgctgacgt tgctgttact cggcggaatg
    11761 tttatggcaa cgcggacggc atttggacga cgcatttatg ccatcggcgg caatctggaa
    11821 gcagcacgtc tctccgggat taacgttgaa cgcaccaaac ttgccgtgtt cgcgattaac
    11881 ggattaatgg tagccatcgc cggattaatc cttagttctc gacttggcgc tggttcacct
    11941 tctgcgggaa atatcgccga actggacgca attgcagcat gcgtgattgg cggcaccagc
    12001 ctggctggcg gtgtgggaag cgttgccgga gcagtaatgg gggcatttat catggcttca
    12061 ctggataacg gcatgagtat gatggatgta ccgaccttct ggcagtatat cgttaaaggt
    12121 gcgattctgt tgctggcagt atggatggac tccgcaacca aacgccgttc ttgattttga
    12181 taaaaatttt ctcaaagccg gttacgtatt accggttttg agtttttgca tgattcagca
    12241 ggaaaagaac catgtttact aaacgtcacc gcatcacatt actgttcaat gccaataaag
    12301 cctatgaccg gcaggtagta gaaggcgtag gggaatattt acaggcatca caatcagaat
    12361 gggatatttt cattgaagaa gatttccgcg cccgcattga taaaatcaag gactggttag
    12421 gagatgtcgt cattgccgac ttcgacgaca aacagatcga gcaagcgctg gctgatgtcg
    12481 acgtccccat tgttggggtt ggcggctcgt atcaccttgc agaaagttac ccacccgttc
    12541 attacattgc caccgataac tatgcgctgg ttgaaagcgc atttttgcat ttaaaagaga
    12601 aaggcgttaa ccgctttgct ttttatggtc ttccggaatc aagcggcaaa cgttgggcca
    12661 ctgaacgcga atatgcattt cgtcagcttg tcgccgaaga aaagtatcgc ggagtggttt
    12721 atcaggggtt agaaaccgcg ccagagaact ggcaacacgc gcaaaatcgg ctggcagact
    12781 gactacaaac gctgccaccg caaaccggga ttattgccgt tactgacgcc cgagcgcggc
    12841 atattctgca agtatgtgaa catctacata ttcccgtacc ggaaaaatta tgcgtgattg
    12901 gcatcgataa cgaagaactg acccgctatc tgtcgcgtgt cgccctttct tcggtcgctc
    12961 agggcgcgcg gcaaatgggc tatcaggcgg caaaactgtt gcatcgatta ttagataaag
    13021 aagaaatgcc gctacagcga attttggtcc caccagttcg cgtcattgaa cggcgctcaa
    13081 cagattatcg ctcgctgacc gatcccgccg ttattcaggc catgcattac attcgtaatc
    13141 acgcctgtaa agggattaaa gtggatcagg tactggatgc ggtcgggatc tcgcgctcca
    13201 atcttgagaa gcgttttaaa gaagaggtgg gtgaaaccat ccatgcaatg attcatgccg
    13261 agaagctgga gaaagcgcgc agtctgctga tttcaaccac cttgtcgatc aatgagatat
    13321 cgcaaatgtg cggttatcca tcgctgcaat atttctactc tgtttttaaa aaagcatatg
    13381 acacaacgcc aaaagagtat cgcgatgtaa atagcgaggt catgttgtag cgatgatgag
    13441 aattgtcggc gtcacatcag ggaactcagc acagccacct gatgcgagca aaagccttat
    13501 ccgaccgaca aatcagataa ggctcgagga aatgaaatta catatgcgcc gcgattaacc
    13561 gttggttatc ctggtacatt gcgaacaggt agttgttata actcttccct ttggtcgagt
    13621 agcccttcag cttgtgaatc atcgctgtgg cagtcacttc ctgatccgct ttacgcagct
    13681 gtgcacgcga tttacggaac gaagagtaag ccgggtgcgt attcaggtta gtgacatagg
    13741 cgctcaccga ttctttgaca gaactaaact gtgagtaccc tttcacttta cccggcgcat
    13801 tggtacaacg tcctttcatg catttcatgc cgaacagatt gttattgttg cgcgccagct
    13861 tcgacgttcc ccaaccgctt tctgctgcag ccatcgtcgc caccatactg gtagggataa
    13921 tgtctacgcg ttcaagcaag gtattccacg ggatttttcg cgtattacca gaccacttca
    13981 ccttgtagcg tttggcgatg tctttcagac gcgcacgctc agcaggtgcc cattggccct
    14041 gatactgttt tgaaatcagc cagttacgtt ctgcagtaat ggctgcattt tggctggtaa
    14101 tgtaaggcat tacggtccgg agaaacgcct ttttccttgg tgttccggaa gggtattttc
    14161 gcaaatcagg aagtgaacta ctctttgcac tattgcgaga atactcttgt ttactgctta
    14221 cctgtttatt actagcttta gttaagtggg acttttgact cgctgttgtt gtgtgcgtct
    14281 tcgctaacac ctcactcgaa aacaccagag tgagtaacat aagaatcatc gccccatatc
    14341 gtcgtatggg agtcaaaatc atcaggtctc ctggtcggat ttaatcattc caacacctta
    14401 tatttttcac aaatttgaga gttgaatctc aaatcatatc aaaaatagct gtcaagagca
    14461 ccccaaggaa tagtccaaat ctgaaactat gtcacgagtt aacgattcag attggcgcta
    14521 aatcgcagaa aatgtggggg ttatcgcaaa attcagccgt tttttgcgcg cgatcgctca
    14581 cccttgcttc tcatcctgtg gacttaccgc tcagggatga gttttgtctg gcttatcgct
    14641 ggcaaactgt ctgaaatcgc agcaataagg actcatccac catgaaactc gccgcctgtt
    14701 ttctgacact ccttcctggc ttcgccgttg ccgccagctg gacttctccg gggttccctg
    14761 cctttagcga acagggaacg ggaacatttg tcagccacgc gcagttgccc aaaggtacgc
    14821 gtccactcac gctaaatttt gaccaacagt gctggcagcc tgcagatgcg ataaaactca
    14881 atcagatgct ttccctgcaa ccttgtagca acacgccgcc tcaatggcga ttgttcaggg
    14941 acggcaaata tacgctgcaa atagacaccc gctccggtac gccaacattg atgatttcca
    15001 tccagaacgc cgccgaaccg gtagcaaacc tggtccgtga atgcccgaaa tgggatggat
    15061 taccgctcac gctggatgtc agcgccactt tcccggaagg agccgccgta cgggattatt
    15121 acagccaaca aattgcgata gtgaagaacg gtcaaataac gttacaaccc gctgctacca
    15181 gcaacggttt actcctgctg gaacgggcag aaactgacac atccgcccct ttcgactggc
    15241 ataacgccac ggtttacttt gtgctgacag atcgtttcga aaacggcgat cccagtaatg
    15301 accagagtta cggacgtcat aaagacggta tggcggaaat tggcactttt cacggcggcg
    15361 atttacgcgg cctgaccaac aaactggatt acctccagca gttgggcgtt aatgctttat
    15421 ggataagcgc cccatttgag caaattcacg gctgggtcgg cggtggtaca aaaggcgatt
    15481 tcccgcatta tgcctaccac ggttattaca cacaggactg gacgaatctt gatgccaata
    15541 tgggcaacga agccgatcta cggacgctgg ttgatagcgc acatcagcgc ggtattcgta
    15601 ttctctttga tgtcgtgatg aaccacacca gctatgccac gctggcggat atgcaggagt
    15661 atcagtttgg cgcgttatat ctttctggtg acgaagtgaa aaaaacgctg ggtgaacgct
    15721 ggagcgactg gaaacctgcc gccggacaaa cctggcatag ctttaacgat tacattaatt
    15781 tcagcgacaa aacaggctgg gataaatggt ggggaaaaaa ctggatcaga acggatatcg
    15841 gcgattacga caatcctgga ttcgatgatc tcactatgtc gctggccttt ttgccggata
    15901 tcaaaaccga atcaactacc gcttctggtc tgccggtgtt ctataaaaac aaaacggata
    15961 ctcacgctaa agtcatcgaa ggctttacac ctcgcgatta cttaacccac tggttaagtc
    16021 agtgggtccg cgactatggg attgatggtt ttcgggtcga taccgccaaa catgttgagt
    16081 tgcccgcctg gcagcaactg aaaaccgaag ccagctccgc gcttcgcgaa tggaaaaaaa
    16141 ctaaccccga caaagcatta gatgacaaac ctttctggat gaccggtgaa gcctggggcc
    16201 acggcgtgat gcaaagtgac tactatcgcc acggcttcga tgcgatgatc aatttcgatt
    16261 atcaggagca ggcggcgaaa gcagtcgatt gtctggcgca gatggatacg acctggcagc
    16321 aaatggcgga gaaattgcag ggtttcaacg tgttgagcta cctctcgtcg catgataccc
    16381 gtctgttccg tgaagggggc gacaaagcag cagagttatt actattagcg ccaggcgcgg
    16441 tacaaatctt ttatggcgat gaatcctcgc gtccgttcgg tcctacaggt tctgatccgc
    16501 tgcaaggtac acgttcggat atgaactggc aggatgttag cggtaaatct gccgccaacg
    16561 tcgcgcactg gcagaaaatc agccagttcc gcgcccgcca tcccgcaatt ggcgcgggca
    16621 aacaaacgac actttcgctg aagcagggct acggctttgt tcgtgagcat ggcgacgata
    16681 aagtgctggt catctgggct gggcaacagt gacttttccg gcttcccgtt cgtcagtacc
    16741 tcgggaagcc gccaaccagg ataaaatgtc agccctaatc agcgttgcgg gataaagcac
    16801 cgctcactct tcaacagacc gatttgcacc ccagcaaatg tagcgttatt gttaccttcc
    16861 ttgctacaga gttcgacaga tatcccgcta tgacattctc cctttttggt gacaaattta
    16921 cccgccactc cggcattacg ctgctgatgg aagatctgaa cgacggttta cgcacgcctg
    16981 gcgcgattat gctcggcggc ggtaatccgg cgcagatccc ggaaatgcag gactacttcc
    17041 agacgctact gaccgacatg ctggaaagtg gcaaagcgac tgatgcactg tgtaactacg
    17101 acggtccaca ggggaaaacg gagctactca cactgcttgc cggaatgctg cgcgagaagt
    17161 tgggttggga tatcgaacca cagaatattg cactaacaaa cggcagccag agcgcgtttt
    17221 tctacttatt taacctgttt gccggacgcc gtgccgatgg tcgggtcaaa aaagtgctgt
    17281 tcccgctggc accggaatac attggctacg ctgacgccgg actggaagaa gatctgtttg
    17341 tctctgcgcg tccgaatatt gaactgctgc cggaaggcca gtttaaatac cacgtcgatt
    17401 ttgagcatct gcatattggc gaagaaaccg ggatgatttg cgtctcccgg ccgacgaatc
    17461 caacaggtaa tgtgattact gacgaagagt tgctgaagct tgacgcgctg gcgaatcaac
    17521 acggcattcc gctggtgatt gataacgctt atggcgtccc gttcccgggt atcatcttca
    17581 gtgaagggcg cccgctatgg aatccgaata tcgtactgtg catgagtctt tccaagctgg
    17641 ggctgcctgg ctcccgctgc ggcattatca tcgccaatga aaaaatcatc actgccatca
    17701 ccaatatgaa cggcattatc agcctggcac ctggcggtat tggtccggcg atgatgtgtg
    17761 aaatgattaa gcgtaacgat ctgctgcgcc tgtctgaaac agtcatcaaa ccgttttact
    17821 accagcgtgt tcaggaaact atcgccatca ttcgccgcta tttaccggaa aatcgctgcc
    17881 tgattcataa accggaagga gccattttcc tctggctatg gtttaaggat ttgcccatta
    17941 cgaccgagca gctctatcag cgcctgaaag cacgcggcgt gctgatggtg ccggggcaca
    18001 acttcttccc gggactggat aaaccgtggc cgcatacgca tcaatgtatg cgcatgaact
    18061 acgtaccaga gccggagaaa attgaggcgg gggtgaagat tctggcggaa gagatagaaa
    18121 gagcctgggc tgaaagtcac taaaaattgc ctgatgcgct acgcttatca ggcctacatg
    18181 atttctgcaa tatattgaat ttggaagaat ttgtaggccg gataaggcgt ttacgccgca
    18241 tctggcataa acaaagcgca ctattccggc attgagaaac gccggaaaac gtttcaaaca
    18301 ggctgctgct gtagccgatg ccgctgcact ttctcgacat ccatgcactg caacgccttt
    18361 gtcgggcagg cttcaacaca ggccggcccc gtctcccgat gccagcacag atcgcactta
    18421 atcgccctcg cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc
    18481 atacagcttt tacagccaat gcaacgtgtt tgttcaacga aaatatgccc atgttcgcgg
    18541 cttatcgcgt caacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct
    18601 actggcgtgg tccagctgtg gtctttaatg acacgaatac gggaaataaa ctcgtctggt
    18661 gacaacgcag cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaggta
    18721 cggcaaccga tacatttcgt cgcatccgca ataataaacc ggttcatgat cattcctttg
    18781 cactaaaaca gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat
    18841 gttaaagttt tggcacttgt ccaaagcgtt atgtgatcgc gcccaggtca tccctgacag
    18901 taaatcccag ttcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt
    18961 tctctcccac ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc
    19021 gcccatgaat atcaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc
    19081 tgtccatcgc cgctccgctt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct
    19141 cggtaatggt attgcgggtt aacggctgga tctcatgctg atggttttcc cagtatgact
    19201 tcacgtagtc cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg
    19261 gcatatgctg gccaatatag gcacgggttc gcagcatccc ggttgtgggt tccagcttat
    19321 aaatcaaaat agcgtgatcg tcttcgcggc tggagaagtt aatggtttca ccagtggcga
    19381 tgttcagtgc ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gacagcgcct
    19441 tctgcccgac ggcaataaat ttggtagtca ggcggtaact ccctgcggcg ggcgcggtgg
    19501 tcacatagcc gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac
    19561 cagccagctc cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa
    19621 tcagcatcaa cccgcgaaac agactctggc ttccggctgg acgctctttt tcctgcgcca
    19681 tctcgttctc tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg
    19741 caaagtgtgc cgtagttcac gatctcgaca gataatttat aaccacttga tttttatgtc
    19801 ttttgaaatt catcaatcag attgctttgt taaaaagtga tcgatatatt tgaaatctag
    19861 ttccgcatat tgaaatttta agccaaaaaa gcgatcaaaa aacaaggaag cctaggatga
    19921 aagtgacatt tgagcagtta aaagcagcct ttaatcgggt cttaatttca cgcggcgttg
    19981 acagcgaaac ggctgacgcc tgtgcagaga tgttcgcccg caccaccgaa tccggcgttt
    20041 attctcacgg cgttaatcgt ttccctcgtt tcattcaaca actggaaaac ggcgatatca
    20101 ttcctgatgc ccaacccaaa cgtataacca gcctcggcgc aattgaacag tgggatgccc
    20161 agcgttcgat cggcaacctg acggcgaaaa agatgatgga tcgcgccatt gaactggctg
    20221 ccgatcacgg tattggtctg gtggcacttc gtaatgccaa ccactggatg cgcggcggca
    20281 gctacggctg gcaggcggcg gaaaaaggct atattggcat ttgctggacc aactccatcg
    20341 ccgtaatgcc gccgtggggc gcaaaagagt gtcgcatcgg cactaacccg ctgatcgtcg
    20401 ccattccttc cacgccgatc accatggtcg atatgtcgat gtcgatgttc tcttacggca
    20461 tgttagaagt taaccgcctg gcaggccgtc agctcccggt cgatggtggc tttgatgatg
    20521 agggcaattt gaccaaagaa cctggcgtta tcgagaagaa tcgccgcatt ttgccgatgg
    20581 gctactggaa aggttctggc atgtcgattg tgctggatat gatcgctact ctcctttccg
    20641 acggcgcatc ggttgccgaa gtca
//
LOCUS       DAJTYH010000071        20659 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTYH010000071 DAJTYH010000000
VERSION     DAJTYH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..112)
                     /locus_tag="OO609_002611"
     CDS             complement(<1..112)
                     /locus_tag="OO609_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HCR6633979.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAI"
     gene            complement(167..1018)
                     /gene="manZ"
                     /locus_tag="OO609_002612"
     CDS             complement(167..1018)
                     /gene="manZ"
                     /locus_tag="OO609_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6633980.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1031..1831)
                     /gene="manY"
                     /locus_tag="OO609_002613"
     CDS             complement(1031..1831)
                     /gene="manY"
                     /locus_tag="OO609_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6633981.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(1894..2865)
                     /gene="manX"
                     /locus_tag="OO609_002614"
     CDS             complement(1894..2865)
                     /gene="manX"
                     /locus_tag="OO609_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6633982.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3328..4878
                     /gene="yoaE"
                     /locus_tag="OO609_002615"
     CDS             3328..4878
                     /gene="yoaE"
                     /locus_tag="OO609_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6633983.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(4882..6480)
                     /locus_tag="OO609_002616"
     CDS             complement(4882..6480)
                     /locus_tag="OO609_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6633984.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6611..7975)
                     /gene="sdaA"
                     /locus_tag="OO609_002617"
     CDS             complement(6611..7975)
                     /gene="sdaA"
                     /locus_tag="OO609_002617"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6633985.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8159..8737)
                     /locus_tag="OO609_002618"
     CDS             complement(8159..8737)
                     /locus_tag="OO609_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6633986.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(8741..10102)
                     /gene="pabB"
                     /locus_tag="OO609_002619"
     CDS             complement(8741..10102)
                     /gene="pabB"
                     /locus_tag="OO609_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6633987.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10176..10355
                     /locus_tag="OO609_002620"
     CDS             10176..10355
                     /locus_tag="OO609_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6633988.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10475..10834)
                     /locus_tag="OO609_002621"
     CDS             complement(10475..10834)
                     /locus_tag="OO609_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6633989.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11196..11540)
                     /locus_tag="OO609_002622"
     CDS             complement(11196..11540)
                     /locus_tag="OO609_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6633990.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11672..13582
                     /locus_tag="OO609_002623"
     CDS             11672..13582
                     /locus_tag="OO609_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6633991.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13640..14335
                     /gene="tsaB"
                     /locus_tag="OO609_002624"
     CDS             13640..14335
                     /gene="tsaB"
                     /locus_tag="OO609_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6633992.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14375..14956
                     /gene="yeaY"
                     /locus_tag="OO609_002625"
     CDS             14375..14956
                     /gene="yeaY"
                     /locus_tag="OO609_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6633993.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15162..16847
                     /gene="fadD"
                     /locus_tag="OO609_002626"
     CDS             15162..16847
                     /gene="fadD"
                     /locus_tag="OO609_002626"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6633994.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            16929..18044
                     /gene="rnd"
                     /locus_tag="OO609_002627"
     CDS             16929..18044
                     /gene="rnd"
                     /locus_tag="OO609_002627"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6633995.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18098..19063)
                     /gene="yeaX"
                     /locus_tag="OO609_002628"
     CDS             complement(18098..19063)
                     /gene="yeaX"
                     /locus_tag="OO609_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6633996.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19119..19736)
                     /gene="yeaW"
                     /locus_tag="OO609_002629"
                     /pseudo
     CDS             complement(19119..19736)
                     /gene="yeaW"
                     /locus_tag="OO609_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20078..20644)
                     /locus_tag="OO609_002630"
     CDS             complement(20078..20644)
                     /locus_tag="OO609_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6633997.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT         5075 a         5145 c         5342 g         5097 t
ORIGIN      
        1 gaattgccag cagggtgggg ccgttacggc ggggcatgat gaactgatcg tagatcgcga
       61 aggccaggag tgcggcgatg aaaagaatca gtaccaggtc cgtgattgtc attaatcctc
      121 cagataaaaa aacggggcca aaaggccccg gtagtgtacg acagtcttac agtcccagca
      181 ggccgcaagc gtaaccagcg ataccgatga cgaagaagcc aacgatgatc cacagcgggt
      241 taactttttt gcgcagcagc cacatacaag caaatgtcag cagcagtggt acaaggcctg
      301 gcattaactg gtcaagaata gtttgcacgg tggtgacacg ggtctgtcca tcctgcccgg
      361 tgatggtcga aaccaccagc gggatgttga catgtgtcca cttgttaacc aatgccccca
      421 tgacaaacag gccgaggata gacgcccctt ccgtcagttt ttgcaggaag ccaccaccca
      481 tatctttaac gatatcgata cctttggagt aaccatacgc tacgccgtag taacgggttg
      541 ccagacgcac caggttaaac aggatgaaga acagcagcgg acctaacagg ctgccgctca
      601 tcgcgatacc ggcacccagt gctgcaaata ccggacgtac ggttccccag aagatcgggt
      661 cgcctacacc agccagcggc cccatcaaac cgactttgat accgttgata gcaccgtcat
      721 cgatctctgc gccattagca cgctgttctt ccagcgccag ggttacgccg agaatcggtg
      781 cagccacgaa cggctgggtg ttaaagaact ccaggtgacg gcgaatagct tgtttacgag
      841 cttcgttgtt ctcagggtag aggcgacgaa ttgccggtac catagagaag cagaaaccca
      901 gtgcctgcat acgttcgaag ttccatgaac cctggaagag gttagaacgc aggaatacgc
      961 cacgaatatc actttgagtg agttttttct cggtggtagt ttgagttgta tcaaccattt
     1021 cgctcacctg ttagtccagt tcgttatcga gatcgttgtt accagctgcc tgagcaggcg
     1081 caccggctac gcggttgtat ttcgggctaa gttggatgta gagcactgcc ataacagtac
     1141 caatcacacc cagagcaacc aggttaaagt tggtgaatgc tgcggttacg aagccgaggt
     1201 agaagaacgg catcaggtag ccagcacgca tcatgttgat aaccatcgca taaccaacca
     1261 ccacgatcat gcccccggcg atattcagac cattggtcac cacttccgga atcgcattca
     1321 gcatgttctg tacttcgctg gtaccaacag acagcgcaac gatgacggcc ggaatagcca
     1381 cacgcattgc ttgcaggaac agagaagaaa cgtggatcca ggaaatcgct gtcaggttgc
     1441 cgttatcagc agccttatcc gcagcgtgct ggaaagcaac ggtaatagta cgaacgatga
     1501 tggtcagtac ctggcccgca gcggccagag ggattgccag tgcgatacct gcaccaatgc
     1561 tctgatgacc tgcgataacc agaatggtag aaatgataga agccagagcg gcgtcaggcg
     1621 caactgcagc accgatgttc atccagccca gcgcgatcat ttccagcgta ccaccgataa
     1681 taataccggt tttcatatcc ccaagaacga tacccaccag ggtacacgcg attagcggac
     1741 ggtgaaactg aaattcatcg aggattgatc ccatacctgc gatacaggct acgataaata
     1801 ccagcacaat ttgaagagtg gtaatctcca ttgtacttct cctgtttacg taagacttaa
     1861 gtgtgaaaac tgaatgataa tcaacacaat acgttactta tcgattttgc tgatcagatc
     1921 catcattttc agtttcggat cggtggaaac cttacggact tccagctcaa taccgcgtgc
     1981 attcagtttt ttgaacgcct cgatatcttt ttcatcaacc gaaaccgcgt tattcacctg
     2041 ggttttaccc tgacggaatg ccataccacc gacgttaaca gaggtgattt tcacgccgcc
     2101 ttcaacgaga cgctctacat ctgttgggtt ggtaaataac agcattacgc gttcgccagc
     2161 atatttcggg ttgttgtaga cgcgaatcat tttggcaaca tcaactacgt gtgctgttac
     2221 gcccggaggt gcaacctggg tgagcagtgt cttacgaacg gtatccgcag ccacttcatc
     2281 actaacaaca ataatacggg agacattggt ttctttggtc cagcgggtgg cgacctgacc
     2341 gtgaatcaga cggtcgtcga tacgcgcaag gccaataacc atgtagtcgt ttggccccat
     2401 tggttttgcc ggagttggag ccgcttttgg tgctgcggca gcgggtgctg gcgcggcttt
     2461 ttcaaccggt ttggctttca gtgctttcac gccttcacgg cctgtttcta ctgccagcgc
     2521 aaccagttca tcaaagctcg ggtcatcatc acgggccatt aacgtttcca cgagcattgg
     2581 aatgttaacg cctgcaatga cttcataatg ctctttgtcg acgacaatgc ggctggcagc
     2641 attgaacggg ctgcctcccc atgtatcaac gagaaacagc acgcctttag tggtgtcgag
     2701 ttttgccaac tgagcgttgt atttttcaat cagcgtttcg gcattttcac ctggaacgaa
     2761 atcgatccag ccgacgtttt cctgctcgcc taacagcatt tctgccgttt taagcaactg
     2821 ctctgcagcc caaccatgtg tgcctataac aatagcaatg gtcacttgct acctccttta
     2881 ttatcgttaa cacctcaacg tgccagatgt atttttgaat cgtcgtccac aatcgaatcg
     2941 attcagataa gggctacaac caaaaacccc tgcgtttcgc gatttatttt agatatcgaa
     3001 aaaattattt tatgtgatga agatccgtaa tttaactttc gattatcaga aatttcgaaa
     3061 ggtaaaatat ccttgtcaca ttcgtttgca aaggaaggta aatctttgcc aaatcagagg
     3121 cgtctctgat atgtttaact cccgtttaac aaccatggag tatagggcag tagcccgcag
     3181 tatggatcgt caccgacgtc atttcagcat caggcctttt aacgcctgcc tttctggcac
     3241 tctatgccgc acctttcgtt tgcattttgt cgttacgcct gcattatttc tggcgtcgaa
     3301 tagctattcc ttaagcagga gcttgtcatg gaattcttaa tggacccctc aatttgggcg
     3361 gggctactca cgcttgttgt tctcgaaatt gtgctgggta tcgataacct ggtcttcatc
     3421 gccattcttg ctgacaaact gccgccaaaa caacgcgata aagcgcgttt gctggggtta
     3481 tcactggcgc tgattatgcg tctggggctg ctgtcgctga tttcatggat ggtcacgctg
     3541 accaaaccgc tatttaccgt catggatttc tccttctccg gacgcgatct gattatgttg
     3601 ttcgggggga tattcttgct gttcaaagca acaaccgaac tgcatgaacg gctggaaaac
     3661 cgcgatcatg attccggcca cggtaaaggc tacgccagtt tctgggtggt cgtcacacag
     3721 attgtcatcc ttgacgccgt cttctcgttg gatgcggtaa ttactgcagt agggatggtt
     3781 aaccatctgc cggtgatgat ggcggcggta gtgattgcga tggcggttat gttgctggca
     3841 tcgaaaccgc tgacgcgatt cgttaaccag caccccacgg tggtggtgct ctgtctgagc
     3901 ttcctgttaa tgattggtct gagtctggtg gcagaaggtt tcggtttcca cattccgaaa
     3961 ggttacctgt atgccgcgat tggcttctcg atcattatcg aagtgtttaa ccagattgcg
     4021 cgtcgcaact ttattcgtca ccagtcgact ttgccgctgc gagcgcgtac tgccgatgcc
     4081 atcctgcgtt tgatgggcgg gaaacgtcag gccaatgttc agcacgatgc cgataacccg
     4141 atgccgatcc cggaaggtgc atttgccgaa gaagaacggt acatgattaa cggcgtactg
     4201 acgctggcgt cgcgttctct gcgcgggatc atgacgccgc gcggtgaaat aagctgggtt
     4261 gacgctaatc tcggggtcga tgaaatccgc gagcaactgc tctcttcacc gcacagtctg
     4321 ttcccggtat gtcgcggtga actggatgaa atcatcggta tcgtacgtgc taaagaactg
     4381 ctggtggcgc tggaagaggg cgttgatgtg gcggcgattg cttcggcgtc tccggcgatt
     4441 atcgtcccgg aaaccctcga tccgatcaac ctgctgggcg tgctgcgtcg cgctcgcggg
     4501 agctttgtta tcgtgaccaa cgagtttggt gtggtacaag gcttggtcac gccgctggat
     4561 gtgctggaag ccattgcggg tgaattcccg gacgctgacg aaacgccgga aatcattacc
     4621 gatggtaacg gctggctggt aaaaggcggt acagatttgc atgccttgca gcaggcgctt
     4681 gatgttgagc accttgccga tgacgatgat atcgcgacgg tcgcgggcct cgtgatctcg
     4741 gcaaatggtc acattccccg tgtgggcgat gtgattgatg tagggccact gcatatcacc
     4801 atctttgaag ccaatgatta tcgtgttgat ctggttcgca ttgttaaaga gcaaccggcg
     4861 cacgatgaag atgagtaagc gttaacgtaa cgacataatg ggcgtgatat gtccattatg
     4921 ccgggcgggc ggcggttggc tgcccgccag ccatttggga aaatcacgta gcggcatcgg
     4981 ctgtgcgtac aaataccctt gcaaaatatg cacgccatga cggcgcaaat acttcgcctg
     5041 ttcttgcgtt tccacgccct ccgccaccag ttcaatattc agtctttgcc ccagcgcgat
     5101 gatgatatcg gtcaccgtcg agttaaccgc gtcagaacct atagctgcgg taaatgactt
     5161 atcaattttc agcacgtcag gacgtaatgt ttcaagccag gaaaacgagc tgttgccggt
     5221 gccgaagtca tcaatcgcca gtttgacgtt tttacgatgc agctcgcggg caatccgata
     5281 atcaacatcc agtaaggcat cgcgttcggt gatttcgagg atcagttgct gaatcgggtg
     5341 agcgctaaac cagtactgat tgagatcttt tatcagcaca ccacggcgaa aatggctggg
     5401 tgcgacgtta atgccaacat gaaactgact actcatcggg aaaacatggt gctgacgaat
     5461 ggtttctgcc atcacatagt gggtcagtgg cacaattaaa tgatgttctt ccgcgatagg
     5521 aataaacaca tccggtgaaa tccagccctg acgcggatta ttccagcgca gcaaaatctc
     5581 tacaccaata cattgctggc tgcgcgcatt aagcaaaggc tgacagaaca attcgaactc
     5641 atgttgcgcc aggcccagat taatttcgcg ggaaaagctc atccggtaag cggtcgccag
     5701 ccaggcgata tagcccacca gtaaacttag cagcaccgcc agcggtaatt gtgttggaag
     5761 atagtgccat gccagcgccg tagcccccgg accatttacg ttaatggtaa aaggaaagct
     5821 ttgcgaagaa acctgatagt tttcattgtc ttcaggttgc ggaagggaat ctaccagccc
     5881 attaccatag agcaaatgcc gtttgtccac cgtcaggctg gcgctactga tttgcggcag
     5941 ttgtggctca aggagcattg ccgtcagtaa gtcgatgtta atcatctcta tgaccccagc
     6001 attgctactg cccgctgcag gcgtccattg aatcaaaacc ggactgcctt taatcagggc
     6061 acggtcgatc gtcaggcgta aaagtggttg cggtgcagga agttcagcca gaatgtccac
     6121 gacgggcaca ttgcgataac caaaaatgct ggagcaataa agtgtgccgt cttgcaccag
     6181 gccaatggat cgcacagttt ggagttttgc cgcctgttta cgtaatggca aatgggcgac
     6241 agagcagggc agaccaatca gcggaagcaa gactttgcta cctgcctgta ggggaagcag
     6301 tactttatct aattcctcta cagcgtgatt ggcgaattgt actacccgtt gttgatttaa
     6361 gttacgctgt gaaataaatc gcacgctcag ggtcgaagcg agcgtaacga gtgcgcaaat
     6421 cgtacaaaca atcattcgat tacggcgata ggttttaatg atccgttgtg ctttttgcat
     6481 gcgggccacc ctgtaagcgt atggcgacaa acgcctgacg caacagtgga agtgtagtgg
     6541 ggaatattac agcagacgag aaagcgggta taaattcgcc catccgttgc agatgggcga
     6601 gtaagaagta ttagtcacac tggactttga ttgccagacc accgcgtgag gtttcgcggt
     6661 atttggcgtt catgtcctta ccggtttcgt acatcgtttc gatgacctta tccagcgaga
     6721 cgcgcggtgc actggtgcgg cgcagagcca tccgtgcggc gttaatcgcc ttcacagagg
     6781 caatggcatt acgctcaatg cacggcacct gaacctgccc tgcaaccggg tcgcaggtta
     6841 aaccaaggtt gtgttccatg ccaatttccg ccgccacgca aacctgttcc gggctaccgc
     6901 ccagcagttc tgcaagaccc gcagcagcca ttgaacaggc aacacccact tcgccctggc
     6961 aaccaacttc cgcaccggaa atagaggcgt tcattttata caatgcacca atcgcgcccg
     7021 ctgccataaa gtaacgggta tagatgtctg ggctgaccga ttcaataaag tggtcatagt
     7081 aagccagcac tgccggaacg ataccgcagg caccgttggt tggcgcagtt accacacgac
     7141 caccggcggc gttttcttcg ttaactgcca gcgcaaacat gtttacccag tcaatgacat
     7201 tcatcggatc gttagacagt ttatcgctgg aaactaacat ccggcgcagg gcagacgcac
     7261 gacgtggcac gcgcagcggg cctggcagta caccttcggt gttcatcccg cgatcgatac
     7321 atgcctgcat ggtttgccag acatgcgcga aatactcgtc gatctctttc ttgctgtgca
     7381 gcgccagttc gttctgcata gcgagaccag acagcgaata gccggtttca ttacagtacg
     7441 cgagcagttc ggtggcagat ttgaacggat acggcacgct cacttcgttg gcagcatcct
     7501 gaccaaagtg ttcttcatcg acgataaaac cgccgccgat ggaataataa gttttgctgt
     7561 agacgacttc atcgccgtta taggcgtgga tttgcatacc gttttcatgc agcggcaggt
     7621 tgccgttatg aaaacgcatc ccgttgtcgc gcgggaaatc cacttcatgc cgtccctgtg
     7681 ccagcagcag acgttcgcgc tcttttacgt cgcgaataaa accgggaata ctgtcgatat
     7741 ccacggtggc aggttcgtta cctgcaagac ccataataat ggcgatatcg gtgtggtggc
     7801 ctttacccgt cagcgacagt gaaccataaa cgtccacggc aacgcgagta acgctatcca
     7861 gtaagccttt ttcgaccaga tcatcgacga actgtttacc cgccttcata ggccctacgg
     7921 tatgggaaga tgagggacca atccccacct taaacatgtc gaatagacta atcacgataa
     7981 tactcctgac aaggtgactg gacttccagt aacgaactaa taacagcgca tagtgtaaga
     8041 gggaacgcgg cgactggctt aactattcac atgaattaaa ctaatcaaaa accgcgattt
     8101 aactaatatt gcgatgattg tctcaagtag tgttttgtag atgtaaagat aagtatagtc
     8161 agggggtcac accaatttgc agcgccagct cacgaattat gcctgcggtc attccccata
     8221 caaaatactg ttcgtaccag gacagccata cccgatgtga atcgccacgg cggtagatat
     8281 ctaaagggtg ataacgaccc agatgtaatg cctgggcgag cggcatttca aacaccgccg
     8341 agacttcatc ttcactggcg cgatacggca gatcgggcgg gataatgccg accactgggg
     8401 ttacttgata gccagttacg ctatcgacgg gcggcagcac gccgataact tcaacggcgg
     8461 aaggcggtat agcgacctct tcttcagctt cgcgcagcgc ggcggcgata actgatgcgt
     8521 ccgtgtcatc gactgcacct ccagggaatg ccacttgtcc agcgtgttta cgcagatgaa
     8581 ttgaacgctg agtcagcaac aaccccggtt gcggtcgacg gacgatgggg attaacacgg
     8641 cagcctgacg atgatttagg ggttcccggt taatttgcgg gcgcaaaagt tgaaagcgag
     8701 ataaaaaatc atcaagcgtc aggctacggt attccacgtc ttacttctcc agttggcgta
     8761 atatcttatt aactttatca aaagtttcct gatattccgc ttcttcctgg ctatcggcga
     8821 caattccacc gcccgcagag cagtatattt gtccgttaat ggcagtcagc gtgcggatag
     8881 taatgctggt atccatgttg ccgcaaaagc tcaaatagcc aatgctgccg caccaggcat
     8941 tacgtcgctg cggttccagt tcgtcgataa tttccatagc ccgtactttc ggagccccgg
     9001 ttattgagcc accaggaaaa gctgcgcgca gcagatcgct ggcgtgtaac tgttctggta
     9061 gtcgcgccgt tatagtgctg accagatgat gcacggcagg gaagggttcc accacgaaga
     9121 gctctggtac ttttaccgaa ccggctacgg caacacgacc gatatcatta cgcattaaat
     9181 cgacaatcat cagattttcg gcacgatctt tcgctgagtt cgccagtttt tctgcttgtt
     9241 tgctatcttc ctgaggatcg ggcaggcgtg gtagcgtgcc tttaatcggg cgagtctgga
     9301 tttcactatt atcacaaaga ataaaccgct ctggcgaaag gcttaaaatt gcaccctgtt
     9361 caagacgtaa aaaagcgcta aatggcgcgc ggttggcctg attaagctga aggaatgcct
     9421 gccattcatc gccagaatag gtcgcatgaa aacgctgggc gagattcacc tgatagcaat
     9481 caccgctgtg cagatattcc tgtacctggc gaaatttttc gccgtactgc tcgtgggtca
     9541 tattggattg ccagtcggaa gtgagcgtga aatcttcctg cggcgagaat tgctggcttt
     9601 ccagccaggc ccgacgggca ttgacatcat tatgactcag caaagaaact gtatgacgct
     9661 ggtggtcgac aatgagcgcc caatcgtaga tacccactgc catatccggc agaacgatat
     9721 cttgttccgc aatttctggc agtgactcaa aacggcggcc cagatcgtag ccaaacaacc
     9781 ccagtgcgcc gccctgaaat ggcaaatctt cgttatgcgt tgggcgaatg tctgcgcgat
     9841 ccagcacctg ctggagcacc tgtagcgggt catcagtggt cgttgtgcgt ttttcgcttt
     9901 cactaacaac ggtttcttta cctaaagtgg ttaaagtgca aatcggatcg gcgaccacaa
     9961 tatcaaagcg gctatacgga tgatcggcat agccggagtg taaaagcatc gcccacggca
    10021 ggtggcttaa gcgggagaaa taaaattcag cggcgtcctg acgccagggt aaagtaatca
    10081 cagtgggaga taacgtcttc atcagtcctg actctactgg ctatgtgccg ggtagcatga
    10141 aataattagc gccaacaatt tagcaggagt taacaatgtt tgcaggttta ccttcactca
    10201 tccatgaaca gcagcaaaaa gctgtcgagc ggatccagga actgatggca caagggatga
    10261 gtagcggcca ggcaatcgcg ctggtggcag aagagctgcg tgccaaccac tccggcgagc
    10321 taatcgtggc gcgttttgag gatgaagacg agtaacacac taatccgtat cgatttcgtt
    10381 atagaagccg atgatcattt gattgtgaga aaaaggacgc tttttatatg tgattaaaag
    10441 cgtcctgacc catttatgct gatataaatt taactcagac cagtgctttt agttcttcct
    10501 gcatatatgt tgaaaagtat tcaggatttt taatcacaga gcgttcacct gtctccattt
    10561 tttttgccca tccaaccatt ttttccgtga acccagggtt ccagccttcc tggtcagctc
    10621 gcgccgtaat atcagcggca ctcgcgggta ctcccagttc ttttaaatat ttaaagattc
    10681 ccttcgccgt gctttcattc atcgaacttg gtgttggcgc tgatacatcc atggcaccaa
    10741 tgaaatccag ggctttatct attacagcag gcatattctt atccttaaaa ttaaccatgt
    10801 tatcgataag gctatatatg accttaattt gcatctaata tcaaagggat tataacgata
    10861 tggcacgagt taacctgttg acattcaggt gttttaaagg tagcagctca tggtgtgtta
    10921 tccagtaaga tggagaaaat taacggtcag cccgcacgct ataaccagat tgttcagcta
    10981 agaaagaaaa ccagagttgc taatcaaagg gcgaacacaa cggcatggtt atgtttattt
    11041 atatagttag ttttatagat aaaattgaaa ttataaatat aaataatgaa ttagcagaag
    11101 catcaacagt aatatcttat tctctggtcg atttatgtaa aaccgaccag tattatcgat
    11161 aaattatcgt ctgacttatt ttgcttcgat aaagcttaca ccgcagccac aatcttaatt
    11221 tcaactttat acttcgggtt catcaaaccc gcttgtaccg tgcagcgcac cggcgcatga
    11281 cccgcgacaa cccaggcatc ccacgcttta ttcatcgccg cgaagtcgtt tttatcggcc
    11341 aggaaaatgg tggcatccag aatgctcgat ttattgctgc cctgtttttc cagcacggcg
    11401 tcaatctgtg ccagcgtgtt ggcggtttgc tcaaaggcat cggcgtcgag gttttccggt
    11461 acaccagtgt agtagagcgt gttgttgtgg attactacat cagaccagcg ggcttcagca
    11521 tcgatacgaa cgatagtcat cattttgtcc tcattatact tccattttcg tgctgcaaag
    11581 actgccacaa tgtgaggttg ccgtcaccac ttcaactggc gaaagcgccc tcatcctgac
    11641 ataatccctg ttcaaatcaa cagggggtag tgtgacggac gattttgcac cagacggtca
    11701 gctggcgaaa gcgataccag gctttaagcc gcgagaacca cagcgacaga tggcggtagc
    11761 cgtcacccag gcgatagaaa aaggccagcc gctggtggtg gaagcaggaa ccggtacggg
    11821 caaaacctac gcttacctgg ctcctgcgct gcgggcgaaa aagaaagtca ttatctcgac
    11881 cggctcaaaa gcgttgcagg atcagctcta cagccgcgat ttgccaacag tctcaaaggc
    11941 attgaaatat acgggcaacg tggcgctgct gaaagggcgc tcaaactacc tctgcctcga
    12001 acgtctcgaa cagcaggcgc tggcgggggg cgatctgccg gtacaaatct taagcgatgt
    12061 gatcctgctg cgctcctggt ctaatcaaac agtcgatggt gatatcagca cctgcgtcag
    12121 cgtggcggaa gattcacagg cgtggccgct ggtcaccagc accaacgaca actgtcttgg
    12181 cagcgactgc ccgatgtata aagattgctt tgtggtcaaa gcacgtaaaa aagcgatgga
    12241 cgccgatgtg gtggtggtaa accatcatct ctttctggcg gatatggtgg ttaaagagag
    12301 tggatttggc gagttgatcc ctgaagcgga cgtcatgatc ttcgacgaag cccaccagct
    12361 accggacatt gccagccagt attttggtca gtcactctcc agtcgacaac tgctcgacct
    12421 ggcaaaagac atcaccatcg cctaccgcac cgaattaaaa gacacccagc agttacaaaa
    12481 gtgcgctgat cgtcttgccc agagtgcgca ggattttcgt ctgcaactcg gtgagccagg
    12541 ttatcgcggt aacctgcgtg agctgttagc taatccgcaa attcagcggg catttttact
    12601 gctcgatgac accttggaac tttgttatga tgtggcgaaa ctgtcactgg ggcgttccgc
    12661 cttgctggat gcggcatttg agcgcgccac gttgtatcgc acacggctga agcggctaaa
    12721 agagatcaat cagccgggct acagctactg gtacgaatgc acttcgcgcc attttactct
    12781 ggctctcacg ccgctcagcg tggcggataa attcaaagag ttaatggcgc aaaagcccgg
    12841 tagctggatc ttcacctcag caacgctgtc ggtgaacgac gatctgcatc atttcacctc
    12901 gcggcttggg atagaacagg cggagtcgtt gctattgcca agcccgtttg attacagccg
    12961 ccaggcgtta ctctgtgtgc cgcgcaatct gccgcaaacc aaccagccag gttctgctcg
    13021 ccagttagcg gcaatgctgc gaccgatcat cgaagctaac aacggtcgtt gttttatgct
    13081 ttgtacctcg cacgccatga tgcgcgatct ggccgagcag ttccgcgcta ccatgacgct
    13141 tcccgttttg ttgcaggggg aaaccagtaa aggccaactg ttgcagcagt ttgtcagtgc
    13201 cggtaatgcg cttcttgtgg caaccagtag tttctgggaa ggggtggacg tgcgtggcga
    13261 tacattgtca ttggtaatta tcgacaaatt gccgtttacc tcgccggatg atccactgtt
    13321 aaaagcgcgc atggaagatt gtcgtttgcg cggtggcgac ccgttcgatg aagtgcaact
    13381 accagatgcc gtcattactc tcaaacaggg ggtaggtcga ctgattcgcg acgccgacga
    13441 tcgtggcgtg ctggtgattt gtgactatcg gctggtgatg cgtccttacg gcgcgacgtt
    13501 tctcgccagt ctgccgcccg cgccacgcac ccgtgacatt gcccgtgcgg ttcgtttcct
    13561 tgcgatacca tcctccaggt aatttgttac ggagtatggt atgatgcgcg cctttttatt
    13621 gatctaagca cgaagaccca tgcgaattct ggctatcgat accgcgacag aggcctgctc
    13681 tgtcgccctg tggaacgacg gtactgtcaa cgctcatttt gagctttgcc ctcgtgaaca
    13741 tactcaacga atcttaccga tggtgcagga tatcctgacc accagcggaa cttccctgac
    13801 tgatattaac gctctggctt acgggcgcgg ccccggtagc tttaccggcg tgcgcattgg
    13861 tattggcatc gcgcaagggc tggcgcttgg cgcggaatta ccgatgattg gcgtctccac
    13921 gctaatgacg atggcgcaag gggcgtggcg caaaaacggc gcaacacgcg tgctgtcagc
    13981 cattgatgcg cgaatgggcg aagtttactg ggccgaatat cagcgtgatg aaaacggtat
    14041 ctggcacggt gaagaaaccg aagccgtact caaacccgaa ctcgttcatg aacgaatgca
    14101 acagctttcc ggcgaatggg tgacggtagg gacgggctgg caagcctggc cggatctcgg
    14161 taaagagagc gggctggttt tgcgcgatgg cgaagtgtta ctccccgctg cagaagatat
    14221 gctgccgatt gcttgtcaga tgtttgccga gggtaaaacg gtggcggtgg aacatgccga
    14281 gccggtttat ttgcgtaaca acgtcgcatg gaagaaactt ccgggcaaag aatgaatctt
    14341 aagagtatgc cctgagaaaa aaaggagtcg catcatggcg gttcaaaaga atgttatcaa
    14401 aggcatactg gcaggtacgt ttgcgctaat gctgagcggt tgtgtcactg tgccggacgc
    14461 cattaaaggc agcagtacca cgccgcaaca agatttagtt cgggtgatga gtgcgccgca
    14521 gctgtacgtt ggtcaggagg cacgctttgg tggcaaagtg gttgcggtac aaaaccagca
    14581 agggaaaacc cgcctggaaa ttgctaccgt accgctggac agcggagcta ggccgacgct
    14641 gggagaacct tctcgcggtc gcatttatgc cgatgtgaac ggttttctgg acccggtgga
    14701 tttccgtgga caactggtta cggtagtcgg gccaatcacc ggtgcggttg acggcaaaat
    14761 cggcaatacg ccctataaat ttatggtgat gcaagtaacg ggttacaaac gttggcattt
    14821 aacccagcag gtgattatgc cgcctcagcc gattgatcca tggttttatg gcggtcgtgg
    14881 ctggccctat ggctacggcg gatggggctg gtataatccc ggccccgcga gagtacaaac
    14941 agttgtaacc gaataattgc ttgtttttaa agaatgcaga aacagcggct ggtccgctgt
    15001 ttctgcattc ttacggtaaa gataaaaata aatagtgacg cgcttcgcaa ccttttcgtt
    15061 gggtaattat caagctggta tgatgagtta atattatgtt aacggcgtgt atatcatttg
    15121 gggttgcgat gacgacgaac acgcatttta gaggtgaaga attgaagaag gtttggctta
    15181 accgttatcc cgcggacgtt ccgacggaga tcaaccctga ccgttatcaa tctctggtag
    15241 atatgtttga gcagtcggtc gcgcgctacg ccgatcagcc tgcgtttgtg aatatggggg
    15301 aggtgatgac cttccgcaag ctggaagaac gcagtcgcgc gtttgccgct tatttgcaac
    15361 aagggttggg gctgaagaaa ggcgatcgcg ttgcgttgat gatgcctaac ttattgcaat
    15421 atccggtggc gctatttggc attttgcgtg ccgggataat cgtcgtaaac gttaacccgt
    15481 tgtatacccc gcgtgagctt gagcatcagc ttaacgatag cggcgcatcg gcgattgtta
    15541 tcgtgtctaa ctttgcccac actctggaaa aagtggttga taaaaccgcc gttcagcacg
    15601 taattctgac ccgtatgggc gatcagctat ctacggcaaa aggcacggta gtcaatttcg
    15661 ttgttaaata catcaagcgc ttggtgccga aataccatct gccagatgcc atttcatttc
    15721 gtagcgcact gcataacggc taccggatgc agtacgtcaa acccgaactg gtgccggaag
    15781 atttagcctt cctgcaatac accggcggca ccacaggtgt ggcgaaaggc gcgatgctga
    15841 ctcaccgcaa tatgctggcg aacctggaac aggtcaacgc cacctatggg ccgttgctgc
    15901 atccaggtaa agaactggta gtaacggcac tgccgctgta tcacattttt gccctgacca
    15961 ttaactgcct gctgtttatc gaactgggtg ggcagaacct gcttatcact aacccgcgtg
    16021 atattccagg gttggtaaaa gagttagcga aatatccgtt taccgctatc actggcgtta
    16081 acaccttgtt caatgcgttg ctgaacaata aagagttcca gcagctggat ttctccagtc
    16141 tgcatctttc cgcaggcggt gggatgcctg ttcagcaagt ggtggcagaa cgctgggtga
    16201 aactgaccgg acagtatctg ctggaaggtt atggcctgac cgagtgcgcg ccgctggtga
    16261 gcgttaaccc gtatgatatt gattatcata gcggtagcat cggtttgccg gtgccatcga
    16321 cggaagccaa actggtggat gacgacgaca acgaagtacc accgggtcaa ccgggtgagc
    16381 tttgtgtcaa aggaccgcag gtgatgctgg gttactggca gcgtccggat gctacagatg
    16441 agatcatcaa aaatggctgg ttacacaccg gcgacatcgc ggtgatggat gaagaaggat
    16501 tcctgcgcat tgtcgatcgt aaaaaagaca tgattttggt ttccggtttt aacgtttatc
    16561 ccaacgagat tgaagatgtc gtcatgcagc atcctggcgt acaggaagtc gcggctgttg
    16621 gcgtaccttc cggctccagt ggtgaagcgg tgaaaatctt cgtagtgaaa aaagatccat
    16681 cgcttaccga agagtcactg gtgacttttt gccgccgtca gctcacggga tacaaagtac
    16741 cgaagctggt ggagtttcgt gatgagttac cgaaatctaa cgtcggaaaa attttgcgac
    16801 gagaattacg tgacgaagcg cgcggcaaag tggacaataa agcctgagcg ttaagtcagt
    16861 cgtcagacgc cggttaatcc ggcgtttttt ttgacgccca ctaaagagaa aacaatttga
    16921 attaccaaat gattaccacg gacgatgcgc tggcttcttt gtgtgaagcc gtccgtgtct
    16981 ttccggcgat agccctggat actgaatttg ttcgtacgcg cacttattac ccgcagctgg
    17041 ggttgattca acttttcgat ggcgagcatc tggcgctaat tgatcctctc gggatcaccg
    17101 actggtcacc gctgaaagcg atcctgcgcg atccgtccat cacaaaattt ctccatgcag
    17161 gcagtgaaga tctggaagtg ttcctcaatg tctttggcga attaccacaa cccttgattg
    17221 acacgcaaat ccttgctgcc ttctgcggac gcccgatgtc atggggtttc gcttccatgg
    17281 tggaagagta ttccggcgtt acgctggaca agagtgaatc gcgcaccgac tggctggcca
    17341 gaccgctgac cgaacgtcag tgtgaatacg cagcggcgga tgtctggtat ctgttaccga
    17401 tcaccgccaa gcttatggta gaaacggagg cctccggctg gctacctgcg gcgctggatg
    17461 aatgccgcct gatgcaaatg cgtcgtcagg aagtcgttgc gccggaagat gcctggcgtg
    17521 atatcaccaa tgcctggcag ttacgcacac gccaactggc ctgtctgcaa ctgttagccg
    17581 actggcgact gcgcaaggcg cgagagcgtg atctggcggt gaactttgtc gtgcgtgaag
    17641 agcatttgtg gtcggtcgcg cgttatatgc cgggaagttt aggcgaactg gacagcctgg
    17701 gtttatccgg tagcgaaatc cgctttcacg gtaaaacgct gctggcgctg gtggaaaaag
    17761 cgcagacatt gccggaagat gccttaccgc agccgatgct taacctgatg gacatgcctg
    17821 gttatcgtaa agcgtttaaa gcgattaagt cgctgattac tgacgtgagc gaaacgcata
    17881 agatcagcgc cgaattgctg gcatcgcgtc ggcaaatcaa ccaactgctg aactggcact
    17941 ggaaactgaa accgcagaac aatttgccgg agctgatttc cggctggcgt ggtgagctga
    18001 tggcggaagc attacacaat ttattgcagg aatatccgca gtaaaatctt ccgaagccgg
    18061 actgggcgcg ctcagcgcca catccggctt tggcaaacta caaatccaac accaggcgtt
    18121 tacccttcgc acgcgaacaa cagatcaaca tactttgctg gctggcacgc tcttcatcgc
    18181 taaaatactg atcccgatgg tcagcttcac cttccagtat tgccgtttcg caggttccgc
    18241 ataccccttc acgacataaa cattccactt tcgcggcttt attattttca ataacctgca
    18301 aaatagtcat ctcttccggc accacaaact cttttccgga acgggcaagc accagggtaa
    18361 atgcatcgcc ggttttgtct tcgatagcaa attgctcaaa gtgcagtgta tcggcggcga
    18421 tgtccagacg cgcagcttca cttcttaccg cttcaattag cgcctcgggg ccacaggtat
    18481 aaacgtgtgt gccaggttcg atatccgcca ataatcgcgc taattccagt cgtgttccgg
    18541 ttgatgaatg atgcaaatgg actttctcag cctgcggatg ctggactagc tcagcacgaa
    18601 atgcgcaact ttctggattt cgcgagcagt aatgtagctg ccagtcgatg tcgctgtgtt
    18661 gcaactctgc catgtgcgac aggaaagggg tgataccaat accgcccacg ataaacagat
    18721 gctttctggc tgagggaatt agcgcaaaca ggttattagg cgttgagatc gttaaccgat
    18781 cgcccacttt tacctgctga tgcaaaaagc gggaaccgcc gcgcgagttt tcttccagtc
    18841 gaacggcaat ctgataacta gaggtgttat gcggtgaact cagtagtgaa tacgcattgc
    18901 tgtactggtt atcaccatcg ctcatctgca ctaggatgtg acttcctccg gtgaacgcag
    18961 gcagcggttt gccatcggtt gccaccagcg tgaagcgttt cacctgttcg gtaaggggtt
    19021 caacctggct cacctgtact tcaaacattt gatagtctga cattgctgta ctccatcaaa
    19081 aatcagccca gcggcggaaa ataccgccgc cgatgtcatt agtccttaaa cacctgcgcc
    19141 agcagattat ggaaatgggc gataccatgt tcggaaatgc cactaccgct actgtcggcc
    19201 atgatgcgcc cctgaccacg atagccacgc gatttcagcc ctttctgtac gctttcaacc
    19261 agacgtaaat cttccggatg gaacacatcg cgataccact caatcagcga tttttgctcg
    19321 tcggttaact cttcattggt gaagtaaata tcgtagtttt gcagggtagt ttcagaatcc
    19381 accgggaatt cataaatgac cgtcatcatc cctttgatcg gggtgacgtt cagcatcgtg
    19441 cacggccaca gccagaaacc gtggaatgcc gcatccgtac cctcttcaaa tttaaacgac
    19501 tgttcggacg gtttggcgaa accgtattgc agcgtccagt taccgtgcat ggtgtgccag
    19561 taacgatcaa cctgtacgga gtcggagaaa cctagatgcg ccggaccaca gtgatagcac
    19621 tcgagatagt tatcgacaat gttcttccag ttggcaggcg tgcgggtggt aaagcgggcc
    19681 gctagtttca gattgtggac ttccgggcag gcttccagca ctttcgcgcc cagaccggtg
    19741 tattgattca cttgaagtac gaaaaaaaac cgggaggaca ttggattatt cgggttctga
    19801 tgggattaga tttggtgggg cttgcaagcc tgtagcgcaa attttagtcg ttaatcaatg
    19861 aaacgcgaaa gatagtaaaa aattgctttt gtttcattga aaatacgaaa aacaaaaaca
    19921 ctgcaaatca tttcaataac agcttcaaaa aacgttcaaa accgataaca accaggctgt
    19981 aaccaaatga ctcatatcac aaatcagctc atgccgttta ggtatgttac atgtgtgatt
    20041 atgtgaggtg aagtatgttt tagctggttc atggttgtta tacggctttt tttacatctt
    20101 gtggttcttg tgaaggagct acaagacctt cctgttcata aataccatac tttgacaaaa
    20161 tctttttacg agtatctgtt ggtaatgatt caagcatttt ggctgtttgt aaggtttgct
    20221 gcgaaatacc ttgcaaatca gcctggatgg aatcatcgga agaaaaaatc tcaggcataa
    20281 gagcttcaaa tcctttcttt ctgaacaact caatcgcaga atgcagttcc ttgtcctcta
    20341 acaattcgac aattgcgatc agtttagttt tcaatgtgct gcggtcaggt tgagaatttt
    20401 tattctcttg attattttta tgctcaacca ttggcatgct ctgactttgg ataaaccact
    20461 ctattggacg ccctgttgcg taggctattt gtgctaatcg atctaaagac gggtatgtct
    20521 tgccagacag gtagtttctg ataaccccat cagaaagtcc gcacttagct gcaaacgccc
    20581 ggcttgattc tccattcatt gcctcttcaa ggcgttcctt gaaacgcatt atttccgggg
    20641 gcataaaaat gcgctctgc
//
LOCUS       DAJTYH010000072        20622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTYH010000072 DAJTYH010000000
VERSION     DAJTYH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            6..770
                     /gene="ycaL"
                     /locus_tag="OO609_002631"
     CDS             6..770
                     /gene="ycaL"
                     /locus_tag="OO609_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6633998.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            943..1626
                     /gene="cmk"
                     /locus_tag="OO609_002632"
     CDS             943..1626
                     /gene="cmk"
                     /locus_tag="OO609_002632"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6633999.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            1737..3410
                     /gene="rpsA"
                     /locus_tag="OO609_002633"
     CDS             1737..3410
                     /gene="rpsA"
                     /locus_tag="OO609_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6634000.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3570..3854
                     /gene="ihfB"
                     /locus_tag="OO609_002634"
     CDS             3570..3854
                     /gene="ihfB"
                     /locus_tag="OO609_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6634001.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4061..6320
                     /locus_tag="OO609_002635"
                     /pseudo
     CDS             4061..6320
                     /locus_tag="OO609_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            6357..8105
                     /gene="msbA"
                     /locus_tag="OO609_002636"
     CDS             6357..8105
                     /gene="msbA"
                     /locus_tag="OO609_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6634002.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8102..9088
                     /gene="lpxK"
                     /locus_tag="OO609_002637"
     CDS             8102..9088
                     /gene="lpxK"
                     /locus_tag="OO609_002637"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6634003.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9125..10357
                     /locus_tag="OO609_002638"
     CDS             9125..10357
                     /locus_tag="OO609_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6634004.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10409..10591
                     /gene="ycaR"
                     /locus_tag="OO609_002639"
     CDS             10409..10591
                     /gene="ycaR"
                     /locus_tag="OO609_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6634005.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10588..11334
                     /gene="kdsB"
                     /locus_tag="OO609_002640"
     CDS             10588..11334
                     /gene="kdsB"
                     /locus_tag="OO609_002640"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6634006.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11488..12381
                     /locus_tag="OO609_002641"
     CDS             11488..12381
                     /locus_tag="OO609_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6634007.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12358..13137)
                     /gene="elyC"
                     /locus_tag="OO609_002642"
     CDS             complement(12358..13137)
                     /gene="elyC"
                     /locus_tag="OO609_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6634008.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            13273..14058
                     /gene="cmoM"
                     /locus_tag="OO609_002643"
     CDS             13273..14058
                     /gene="cmoM"
                     /locus_tag="OO609_002643"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6634009.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14055..15377
                     /gene="mukF"
                     /locus_tag="OO609_002644"
     CDS             14055..15377
                     /gene="mukF"
                     /locus_tag="OO609_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6634010.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15358..16062
                     /gene="mukE"
                     /locus_tag="OO609_002645"
     CDS             15358..16062
                     /gene="mukE"
                     /locus_tag="OO609_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6634011.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16062..20522
                     /gene="mukB"
                     /locus_tag="OO609_002646"
     CDS             16062..20522
                     /gene="mukB"
                     /locus_tag="OO609_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6634012.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
BASE COUNT         4951 a         5021 c         5709 g         4941 t
ORIGIN      
        1 ttcacatgaa gaatactaaa ttactgctgg cgattgcgac ctctgcagca ttactgacag
       61 ggtgtcaaaa tacccacggt attgatacca atatggctat cagctccggt ttaaatgcct
      121 ataaagcagc aacattaagc gatgccgatg caaaagcgat tgccaatcag ggctgtgccg
      181 aaatggacag cggcaatcaa gtcgcaagta aatccagcaa gtacggtaaa cgtctggcaa
      241 aaatcgccaa agcattgggt aacaatatta atggcacgcc ggtcaactat aaggtttata
      301 tgaccagcga cgtcaacgca tgggcgatgg cgaacggctg tgttcgtgtc tacagtggcc
      361 tgatggacat gatgaatgac aacgaaattg aaggtgttct gggccatgaa ctgggccacg
      421 tcgcgttggg tcactcgctg gctgaaatga aagcttctta tgcgatcgtt gccgcacgcg
      481 atgccatttc agctaccagc ggtgtggctt cccagctttc ccgctcacaa ttgggtgata
      541 tcgcagaagg cgctattaat gctaaatact cccgtgataa agagtccgaa gcagatgatt
      601 tctcttttga tctgttgaag aaacgtggca tcagcaccca ggggctggtt ggcagctttg
      661 aaaaactggc tagcctggat ggcggtcgca cccagtccat gtttgactct cacccaccat
      721 caacagagcg tgcgcaacac atccgtgatc gtatcgcctc tggtaagtaa atcattgtca
      781 tctttcgggc tggtcttctg ccagcccgct ataattgcgc aataaatccc catctgaata
      841 cagacaaaac tggtttttgc acacaacgtt aacgatttgt ggcgtcggcg cgtataatgc
      901 gcgcggttat gttaacggta cgcctgtttt aaggagataa agatgacggc aattgccccg
      961 gttattacca ttgatggccc aagcggtgca gggaaaggca ccttgtgtaa ggctatggcg
     1021 gaagcgttgc aatggcatct gctggactcg ggtgcaattt atcgcgtact ggcattggcg
     1081 gcattacatc accatgttga tgttgcgtcg gaagatgcgc tggtaccgct ggcatcccat
     1141 ctggatgtac gttttgtgtc gaccaatggc aatctggaag tgatcctcga aggggaagat
     1201 gtcagcggcg aaattcgtac tcaggaagtg gcgaatgcgg cttcacaagt cgcggcattc
     1261 ccacgcgttc gtgaagcatt attgcgtcgc caacgcgcgt ttcgcgaatt accaggtctg
     1321 attgccgatg gccgcgacat gggaacggtg gtattccctg atgcaccagt gaaaattttc
     1381 cttgacgcct cctcggaaga acgtgcgcat cgccgcatgc tacagttgca ggagaagggc
     1441 tttagtgtta acttcgagcg ccttttggcc gagatcaaag aacgcgacga ccgcgatcgt
     1501 aaccgagcgg tagcgccact ggttccggca gccgatgctt tagtgttgga ttccaccacc
     1561 ttaagcattg agcaattgat tgaaaaagcg ctacaatacg cgcgccagaa attggctctc
     1621 gcataagcga ccgaatttgc agtacccccg ttgcaatgga atgacagcgg gtatgttaaa
     1681 caaccccatc cggcatggag ccaggtggac gttaaatata aacctgaaga ttaaacatga
     1741 ctgaatcttt tgctcaactc tttgaagagt ccttaaaaga aatcgaaacc cgcccgggtt
     1801 ctatcgttcg tggcgttgtt gttgctatcg acaaagacgt agtactggtt gacgctggtc
     1861 tgaaatctga gtccgccatc ccggctgagc agttcaaaaa cgcccagggc gagctggaaa
     1921 tccaggtagg tgacgaagtt gacgttgctc tggacgcagt agaagacggc ttcggtgaaa
     1981 ctctgctgtc ccgtgagaaa gctaaacgtc acgaagcctg gatcacgctg gaaaaagctt
     2041 acgaagatgc tgaaactgtt accggtgtta tcaacggcaa agttaagggc ggcttcactg
     2101 ttgagctgaa cggtattcgt gcgttcctgc caggttctct ggtagacgtt cgtccggtgc
     2161 gtgacactct gcacctggaa ggcaaagagc ttgaatttaa agtaatcaag ctggatcaga
     2221 agcgcaacaa cgttgttgtt tctcgtcgtg ccgttatcga atccgaaaac agcgcagagc
     2281 gcgatcagct gctggaaaac ctgcaggaag gcatggaagt taaaggtatc gttaagaacc
     2341 tcactgacta cggtgcattc gttgatctgg gcggcgttga cggcctgctg cacatcactg
     2401 acatggcctg gaaacgcgtt aagcatccga gcgaaatcgt caacgtgggc gacgaaatca
     2461 ctgttaaagt gctgaagttc gaccgcgaac gtacccgtgt atccctgggc ctgaaacagc
     2521 tgggcgaaga tccgtgggta gctatcgcta aacgttatcc ggaaggtacc aaactgactg
     2581 gtcgcgtgac caacctgact gactacggct gcttcgttga aatcgaagaa ggcgttgaag
     2641 gtctggtaca cgtttccgaa atggattgga ccaacaaaaa catccacccg tccaaagttg
     2701 ttaacgttgg cgatgtagtg gaagttatgg ttctggatat cgacgaagaa cgtcgtcgta
     2761 tctccctggg tctgaaacag tgcaaagcta acccgtggca gcagttcgcg gaaacccaca
     2821 acaagggcga ccgtgttgaa ggtaaaatca agtctatcac tgacttcggt atcttcatcg
     2881 gcctggacgg cggcatcgac ggcctggttc acctgtctga catctcctgg aacgttgcag
     2941 gcgaagaagc agttcgtgaa tacaaaaaag gcgacgaaat cgctgcagtt gttctgcagg
     3001 ttgacgcaga acgtgaacgt atctccctgg gcgttaaaca gctcgcagaa gatccgttca
     3061 acaactgggt tgctctgaac aagaaaggcg ctatcgtaac cggtaaagta actgcagttg
     3121 acgctaaagg cgcaaccgta gaactggctg acggcgttga aggttacctg cgtgcttctg
     3181 aagcatcccg tgaccgcgtt gaagacgcta ccctggttct gagcgttggc gacgaagttg
     3241 aagctaaatt caccggcgtt gatcgtaaaa accgcgcaat cagcctgtct gttcgtgcga
     3301 aagacgaagc tgacgagaaa gatgcaatcg caactgttaa caaacaggaa gatgcaaact
     3361 tctccaacaa cgcaatggct gaagctttca aagcagctaa aggcgagtaa ttctctgact
     3421 cttcgggatt tttattccga agtttgttga gtttacttga cagattgcag gtttcgtcct
     3481 gtaatcaagc actaagggcg gctacggccg cccttaatca atgcagcaac agcagccgct
     3541 taatttgcct ttaaggaacc ggaggaatca tgaccaagtc agaattgata gaaagacttg
     3601 ccacccagca atcgcacatt cccgccaaga cggttgaaga tgcagtaaaa gagatgctgg
     3661 agcatatggc ctcgactctt gcgcagggcg agcgtattga aatccgcggt ttcggcagtt
     3721 tctctttgca ctaccgcgca ccacgtaccg gacgtaatcc gaagactggc gataaagtag
     3781 aactggaagg aaaatacgtt cctcacttta aacctggtaa agaactgcgc gatcgcgcca
     3841 atatttacgg ttaagttttt actcaaactt gaacgcgaga aaagcacctg tcgggtgctt
     3901 ttttcaattc tctaatcagg aactggaagc tgcctcgcag agttttgagc agttttcacc
     3961 ctttcgttaa attcttctga atgtgcctcg gggaacgcaa aattcccacc agacaaccgc
     4021 tcaacaaagt tgcacacttt ccataaacag ggaggggtgc atgaaaataa cgacagtcgg
     4081 cgtatgcata atttgcggaa tttttccgtt gctgattttg ccccaattgc ctaggacatt
     4141 aacccttgcg tttctgactc tcttcgcctg cgtactggca tttatccctg ttaaaaccgt
     4201 ccgttatatc gcgctgacgt tgctgttttt cgtttggggc atattatcag caaagcaaat
     4261 tttgtgggca ggagaaacct taactggcgc gacgcaggat gcaattgttg agatcactgc
     4321 aactgacggc atgaccactc attacggtca aattacttat ctacaaggtc gacgtatatt
     4381 ccctgcgcca ggccttgtgc tgtatggcga ataacttccg caagcggttt gtgctggaca
     4441 acaatggtca atgaaactca aagttcgtgc agttcatggc caacttaatg atggcggctt
     4501 tgatagccag cgttatgcca ttgcccagca tcagccgctc accggccgtt ttctgcaggc
     4561 aagtgtcatt gaaccgaatt gtagcctgcg tgcacagtat ctggcgtcac tacaaacaac
     4621 gctgcaaccc tatccgtgga atgcggttat tcttggttta ggtatggggg aacggttatc
     4681 cgtccccaaa gaaatcaaaa atatcatgcg cgatactgga acggcgcatt taatggcgat
     4741 atcgggattg cacatcgctt ttgcggcgtt gctggctgcc ggactcattc gcagtggaca
     4801 aatttttctg cctgggcgct ggatccactg gcaaatacca ttaattggcg gaatctgctg
     4861 tgctgctttt tatgcctggt tgacgggaat gcaacctcct gcattgcgta ccgtggtggc
     4921 gcttgctacg tggggaatgc ttaagttaag tgggcgacag tggagtggct gggatgtatg
     4981 gatatgttgt ctggcggcaa ttttgctgat ggatcctgtt gccattctct cgcaaagttt
     5041 atggctctct gccgctgcgg tcgcggcact gattttttgg tatcagtggt ttccctgtcc
     5101 tgagtggcaa ctgccgccgg tattgcgtgc agttgtttcc ctcatccatc tgcaactggg
     5161 aatcacactc ctgcttatgc ccatgcaaat cgtcatattt catggcatta gtctgacctc
     5221 gtttattgca aatctatttg caattccctt ggtgacattt atcacggttc cgttgatcct
     5281 cgccgcgatg gttgtgcatt ttagcgggcc gttaatcctg gaacaggggt tatggtttct
     5341 tgccgaccgg tctttggctt tacttttctg ggggttaaag agtttgccgg aagggtggat
     5401 caacattgtt gaatgttggc aatggctatc attttcccca tggttcttac tggtggtatg
     5461 gcgattaaac gcctggcgaa cgttgccagc aatgtgtgtg gctggaggct tgctgatgtg
     5521 ctggccgctg tggcaaaaac ctcgacctga cgagtggcag ctgtacatgc ttgatgtcgg
     5581 gcaagggctg gcaatggtga tagccagaaa cggcaaagcg attctctatg acacaggact
     5641 ggcctgaagg ggatagtggg caacaactga ttatcccctg gctccactgg cataatcttg
     5701 aaccggaagg cgttattctg agccatgaac atctggatca ccggggaggg ctggattcaa
     5761 tattgcatat atggccgatg ttatggatca gaagtccgtt aaactgggaa catcatcagc
     5821 cctgtgtgcg tggcgaagcg tggcagtggc aaggattgcg tttcagcgcg cactggcctt
     5881 tacaaggtag caacgataag ggaaataacc attcctgtgt ggttaaggtt gatgacggga
     5941 cgaatagcat tcttctaacc ggtgatattg aagccccagc tgaacaaaag atgctaagcc
     6001 gttactggca gcaagtgcag gcaacattgc ttcaggtacc tcaccatggc agtaatacct
     6061 catcatcgtt gccattaatt cagcgagtga atggaaaagt ggcactcgca tcggcatcgc
     6121 gctataacgc atggcgattg ccctctaaca aagttaagca tcgctatcaa cagaaaggat
     6181 atcaatggct tgatactcca catcaggggc aagtgacggt caatttttca gcacaaggct
     6241 ggcggattag cagcctcaga gagcaaattt tacctcgttg gtatcatcag tggtttggcg
     6301 tgccagtgga taacgggtag aatatgcggc tatttcaaca aatgctggtt ttttgaatgc
     6361 ataacgacaa agatctctct acgtggcaga cattccgccg actgtggcca accattgcgc
     6421 ctttcaaagc gggtctgatc gtggcgggcg tagcgttaat cctcaacgca gccagcgata
     6481 ccttcatgtt atcgctcctt aagccacttc ttgatgatgg ctttggtaaa acagatcgct
     6541 ccgtgctggt gtggatgccg ctggtggtga tcggactgat gattttacgt ggtatcacca
     6601 gctatgtctc cagctactgt atctcctggg tatcaggaaa ggtggtaatg accatgcgtc
     6661 gccgcctgtt tggtcacatg atgggaatgc cagtttcatt ctttgacaaa cagtcaacgg
     6721 gtacgctgtt gtcacgtatc acctacgatt ccgaacaggt tgcttcttct tcttccggcg
     6781 cactgattac tgttgtgcgt gaaggtgcgt cgatcatcgg cctgttcatc atgatgttct
     6841 attacagttg gcaactgtcg atcattttga ttgtgctggc accgattgtt tcgattgcga
     6901 ttcgcgtagt atcgaagcgt tttcgcaaca tcagtaaaaa catgcagaac accatggggc
     6961 aagtgaccac cagtgcagaa caaatgctga agggccacaa agaagtattg attttcggtg
     7021 gtcaggaagt ggaaacgaaa cgcttcgata aagtcagtaa ccgaatgcgt cttcagggga
     7081 tgaaaatggt ttcagcctct tccatctctg atccgatcat tcagctgatc gcctctttgg
     7141 cgctggcgtt tgttctgtat gcggcgagct tcccaagtgt tatggataac ctgactgccg
     7201 gtacgattac cgttgttttc tcttcaatga ttgcactgat gcgtccgctg aaatcgctga
     7261 ccaacgttaa cgcccagttc cagcgcggta tggcggcttg tcagacgctg tttaccattc
     7321 tggacagtga gcaggagaaa gacgaaggta agcgcgtgat cgagcgtgcg actggcgacg
     7381 tggaattccg caatgtcacc tttacttatc cgggacgtga cgtacccgca ttgcgtaaca
     7441 tcaacctgaa aattccggca gggaagacgg ttgctctggt tggacgctct ggttcaggta
     7501 aatcaaccat cgccagcctg atcacgcgtt tttacgatat tgatgaaggc gaaatcctga
     7561 tggatggtca cgatctgcgc gagtataccc tggcgtcgtt acgtaaccag gttgctctgg
     7621 tgtcgcagaa tgtccatctg tttaacgata cggttgctaa caacattgct tacgcacgga
     7681 ctgaacagta cagccgtgag caaattgaag aagcggcgcg gatggcctac gccatggact
     7741 tcatcaataa gatggataac ggtctcgata cagtgattgg tgaaaacggc gtgctgctct
     7801 ctggcggtca gcgtcagcgt attgctatcg ctcgagcctt gctgcgtgat agcccgattc
     7861 tgattctgga tgaagctacc tcggctctgg ataccgaatc cgaacgtgcg attcaggcgg
     7921 cactggatga gttgcagaaa aaccgtacct ctctggtgat tgcccaccgc ttgtctacca
     7981 ttgaaaaggc agacgaaatc gtggtcgtcg aggatggtgt cattgtggaa cgcggtacgc
     8041 ataacgattt gcttgagcac cgcggcgttt acgcgcaact tcacaaaatg cagtttggcc
     8101 aatgatcgaa aaaatctggt ctggtgaatc ccctttgtgg cggctattgc tgccactctc
     8161 ctggttgtat ggcctggtga gtggcgcgat ccgtctttgc tataaactaa aactgaagcg
     8221 cgcctggcgt gcccccgtac cggttgtcgt ggttggtaat ctcaccgcag gcggcaacgg
     8281 aaaaaccccg gtcgttgtct ggctggtgga acagttgcaa cagcgcggta ttcgcgtggg
     8341 ggtcgtatcg cggggatatg gtggtaaggc tgaatcttat ccgctgttat tgtcggcaga
     8401 taccaccaca gcacaggcgg gtgatgaacc tgtgttgatt tatcaacgca ctgatgcgcc
     8461 tgttgcggtt tctcccgttc gttctgatgc ggtaaaagcc attctggcgc aacaccctga
     8521 tgtgcagatc atcgtaaccg acgacggttt acagcattac cgtctggcgc gtgatgtgga
     8581 aattgtcgtt attgatggtg tgcgtcgctt tggcaatggc tggtggttgc cggcggggcc
     8641 aatgcgtgag cgagcggggc gcttaaagtc ggttgatgcg gtaatcgtca acggcggtgt
     8701 ccctcgcagc ggtgaaatcc ccatgcatct gctgccgggt caggcggtga atttacgtac
     8761 cggtacgcgt tgtgacgttg ctcagcttga acatgtagtg gcgatggcgg ggattgggca
     8821 tccgccgcgc ttttttgcca cgctgaagat gtgtggcgta caaccggaaa aatgtgtacc
     8881 gctggccgat catcagtctt tgaaccatga ggatgtcagt gcgttggtaa gcgccgggca
     8941 aacgctggta atgactgaaa aagatgcggt gaaatgccgg gcctttgcag aagaaaattg
     9001 gtggtatttg cctgtagacg cacagctttc aggtgatgaa ccagcgaaac tgcttacgca
     9061 actaacctcg ctggcttctg gcaactagtt acgccgcggc agcgttcgat tgatggagtc
     9121 atgaatgtcg ctgccgcacc tctcccttgc tgatgcgcgt aatcttcacc ttgccgcaca
     9181 aggcctgtta aacaaacccc gccgtcgagc gtcgttggag gatattccgg caacgatctc
     9241 ccgcatgtcc ttgctgcaaa tcgataccat caatattgtt gcccgtagtc catatctggt
     9301 gcttttcagt cgtctgggaa attatcctgc ccagtggctg gatgagtctc tggcgcgtgg
     9361 cgaattaatg gaatactggg cgcatgaagc ctgctttatg ccgcgtagcg actttcgtct
     9421 tattcgccac cgcatgctgg cacctgaaaa aatgggctgg aaatacaaag acgcctggat
     9481 gcaggaacat gaggcggaaa ttgcacagtt aattcagcat attcatgata aagggccggt
     9541 acgttcagcc gattttgagc atcctcgtaa aggtgcaagc ggctggtggg aatggaagcc
     9601 gcataaacgg catctggaag gtttatttac tgccggaaag gtgatggtga ttgaacggcg
     9661 caacttccag cgcgtttatg atttaaccca ccgtgtcatg cctgactggg atgatgagcg
     9721 cgatctcgtt tcgcaaacag aagcagaaat catcatgctg gataacagtg cgcgtagcct
     9781 gggaatattc cgcgaacagt ggctggcaga ttactatcgg ctgaaacgtc cggcactggc
     9841 ggcgtggcgc gaagcgaggg ctgaacagca gcaaatcatt gctgtgcatg ttgaaaaatt
     9901 gggcaatctt tggctgcatg ctgatttgct gccgctactc gagcgtgcgc tggccggaaa
     9961 gctcactgca acgcacagcg cggtactttc gccttttgat cctgttgtct gggatcgcaa
    10021 acgcgcagag cagctttttg attttagcta ccggctggag tgctataccc cagcgccgaa
    10081 acgccagtat ggctattttg ttctgccatt attacatcgt gggcaattag ttgggcgaat
    10141 ggatgccaaa atgcatcgcc agacaggcat ccttgaagtt atctctctgt ggttacagga
    10201 aggcattaaa ccaacgacaa tgctgcaaaa agggttacgt caggcgatta ctgatttcgc
    10261 gagctggcag caggcaacgc gggtgacatt aggacgctgc ccgcaaggtc tctttactga
    10321 ttgccgcact ggctgggaaa tagaccccgt cgcataaatg attatgataa gatttatgca
    10381 ttcataaccc gatccatctg gaggaactat ggatcatcgt ttgcttgaaa tcattgcctg
    10441 cccggtttgc aacggaaaac tttggtataa ccaggaaaaa caagagctta tttgcaaact
    10501 ggataacctg gctttcccgc tccgcgatgg cattccagtg ttgctggaaa cggaagcccg
    10561 cgtgctgact gctgatgaga gtaaatcatg agttttgtgg tcattattcc cgcgcgctac
    10621 gcgtccacgc gtctgcccgg taaaccattg gttgatatta acggcaaacc catgattgtt
    10681 catgttcttg aacgcgcgcg tgaatcaggt gccgagcgca tcatcgtggc aaccgatcat
    10741 gatgatgttg cccgcgccgt tgaagccgct ggcggtgaag tatgtatgac gcgcgccgat
    10801 catcagtcag gaacagaacg tctggcggaa gttgtcgaaa aatgcgcatt cagcgacgac
    10861 acggtgatcg ttaatgtgca gggtgatgaa ccgatgatcc ctgcgacaat cattcgtcag
    10921 gttgctgata acctcgctca gcgtcaggtg ggtatggcga ctctggcggt gccaatccac
    10981 aatgcggaag aagcgtttaa cccgaatgcg gtgaaagtgg ttctcgacgc tgaagggtat
    11041 gcactgtact tctcacgtgc caccattcct tgggatcgtg atcgttttgc agaaggcctt
    11101 gaaaccgttg gcgataactt cctgcgtcat cttggtattt atggctaccg tgcaggcttt
    11161 atccgtcgtt acgtcaactg gcaggcaagt ccgttagaac acatcgaaat gttagagcag
    11221 cttcgtgttc tgtggtacgg cgaaaaaatc catgttgctg ttgctcagga agttcctggc
    11281 acaggtgtgg atacccctga agatcttgag cgcgttcgcg ctgaaatgcg ctaatttcac
    11341 ttcacgacac ttcagccaat tttgggagga gtgtcgtacc gttacgattt tcctcaattt
    11401 ttcttttcaa caattgatct cattcaggtg acatctttta tattggcgct cattatgaaa
    11461 gcagtagctt ttatgagggt aatctgaatg gaacagctgc gtgccgaatt aagccattta
    11521 ctgggcgaaa aactcagtcg tattgagtgc gtcaatgaaa aagcggatac ggcgttgtgg
    11581 gctttgtatg acagccaggg aaacccaatg ccgttaatgg caagaagctt tagtacgccc
    11641 ggaaaagccc gacaactggc atggaaaacc accatgctgg caagaagtgg gactgtccgt
    11701 atgccgacta tttatggtgt gatgacgcat gaagaacacc ccggccctga tgtcctgcta
    11761 ctggagcgga tgcgtggtgt ttcggtggag gcaccagccc gaacaccaga acgctgggaa
    11821 cagctcaaag accaaatcgt tgaagcctta ctggcctggc accgtcagga cagtcgcggt
    11881 tgcgtcggcg cggtcgacaa tactcaggaa aatttctggc cctcatggta ccggcaacat
    11941 gttgaagtgc tatggaccac gctcaatcag ttcaataaca ccggtctgac gatgcaggat
    12001 aagcggatcc tgtttcgcac tcgcgaatgt ctcccggcat tatttgaagg ctttaacgac
    12061 aattgtgtgt tgattcacgg taacttctgt ttacgcagca tgctgaaaga ttcgcgcagc
    12121 gatcagttac tggcgatggt cgggccggga ctaatgcttt gggcaccgcg agaatacgaa
    12181 ctgttccgac taatggataa ttctctggcg gaagatttgc tctggagtta cctgcaacgc
    12241 gcgccagtgg cggagtcgtt catctggcgg cgttggttgt atgtgttatg ggatgaagtt
    12301 gcgcaactgg ttaataccgg acgatttagt cggcgcaact tcgatctggc atcaaaatca
    12361 ctcttgccgt ggctcgcctg acggaccttt cagccactgc cagatgcggc caagcgtttc
    12421 atagccaaca cgatcgctat gcatcagcca gaccggagaa gggattgccc gttcccacgg
    12481 attaagcggc gagtcgatgg ccaactgatt cgccggggct ggtagcggat ttaacccttc
    12541 ctgctgaaaa aagatcatcg cccgcggcag atgtgatgcg gaggtcacca gcaggaaagg
    12601 ggcgtcacca atcgcctgtt tcactgccgc agcttcttct tcggtatctt ttggcaaatc
    12661 cagggtgata atttgctcgc gcggtacgcc cagcgattgc gcaactctgg cacctacttc
    12721 cgctgtactc accgtattgg tttttgctac gcctcccgtg aagatcagtt ttgaacccgg
    12781 attttcgcgc cataaacgga taccctcatt caggcgtggc agactgttat tgattaaatt
    12841 agagctcggt gcccactgcg ggttccaggt ataaccacct ccgagcacca cgatatagtc
    12901 cactttttgc gaattattcc atgtcggata ggtgctttcg atgggacgca gcaggcggtc
    12961 ggctaccggt tgcaaactca ataacaagag tgccagccac ccaatgctaa taaaaatctt
    13021 cccggttttt tgaaaacggc taaaccacag cagcgccagg ccagcgccaa taattaacaa
    13081 catcagcgga agtggcagca acatattgcc aatcactttt ttcagtgtaa aaagcatcct
    13141 ttctggttcc ttttttaacc atatagcaag ggatcgctag cgatattaca ccagacgggt
    13201 tcattctcgc cgtggctgtg acaaaatagc gtctttatca gaaaacgtct tagccaaccg
    13261 gtggagaaag caatgcagga tcgcaatttt gatgatattg cggaaaagtt ttcccgtaac
    13321 atttacggca ccaccaaagg gcagcttcga caggctattc tgtggcagga tctcgatcgc
    13381 gtgctggcgg aaatgggccc gcaaaaactg cgtgtgctgg atgctggcgg cggagaaggg
    13441 cagaccgcaa tcaaaatggc cgagcgcggg catcaggtta ttttatgcga tctttctgcg
    13501 cagatgatcg accgcgcaaa acaggcggca gaagcaaaag gtgtgagcga caacatgcaa
    13561 tttatacatt gcgccgctca ggatgttgct tcgcatttgg aaacgcccgt tgatctgata
    13621 ttgttccatg cggtgctcga gtgggtggcc gatccccgca gtgtattgca gaccctgtgg
    13681 tcagtgttgc gtccaggcgg agtgttgtcg ttaatgttct acaatgcgca tggtttgttg
    13741 atgcataaca tggtcgccgg gaattttgat tacgtgcagg cgggaatgcc gaaaaagaaa
    13801 aaacggacgc tttcgccaga ttatccacgc gacccggcgc aggtttatct gtggctggaa
    13861 gaagctggtt ggcaaattat gggtaagaca ggcgttcgcg tgtttcatga ttatctgcgc
    13921 gagaagtacc agcagcgcga ctgctatgaa gcattacttg aattagaaac gcgttattgc
    13981 cgtcaggaac cgtatattac cctagggcgt tatattcatg tcaccgcgcg caaaccgcag
    14041 agcaaggata aagtatgagt gaattttccc agacagtccc cgaactggtt gcctgggcca
    14101 gaaaaaatga cttctccatc tcgctgccgg tagaccgact ctcttttctg ctggcggttg
    14161 ccacgctgaa cggcgagcgt ctggatggtg agatgagtga aggcgagctg gtggatgcat
    14221 tccgccatgt gagtgatgcg tttgagcaaa ccagcgaaac catcggcgtg cgcgccaata
    14281 acgcgatcaa cgacatggtg cgtcaacgtc tgctgaaccg ctttaccagc gagcaggcgg
    14341 aagggaacgc aatttaccgt ctgacgccgc tcggcatcgg cattactgac tactacatcc
    14401 gtcagcgcga gttttctacg ctgcgtcttt ctatgcagtt gtcgattgtg gcgggtgagc
    14461 tcaaacgcgc agcagatgcc gccgaagagg gcggtgatga atttcactgg caccgtaatg
    14521 tctatgcgcc actgaaatat tcggtagcag aaattttcga cagtatcgac ctgacgcaac
    14581 gtctgatgga cgaacagcag cagcaggtga aggacgatat cgcccagttg ctgaacaaag
    14641 actggcgggc ggcgatttcc agctgtgaat tgttgctttc ggaaacttcc ggaacgctgc
    14701 gtgaattgca ggatacgctg gaagcggcag gcgacaaatt gcaggctaat ctgttgcgca
    14761 ttcaggatgc gacgatgacc catgacgatc tgcatttcgt cgatcgtctg gtgttcgatc
    14821 tgcagagcaa actcgatcgt attatcagtt ggggccagca atccatcgac ttgtggattg
    14881 gctacgaccg ccacgtacac aaatttattc gtaccgcgat cgatatggat aaaaaccgcg
    14941 tctttgctca gcggttacgt cagtcggtac aaacctattt tgatgagccg tgggcgctaa
    15001 cttatgccaa tgccgatcgt ctgctggata tgcgtgacga agagatggca ctgcgcgatg
    15061 aagaagtgac tggggaactt cctgaggatc tggaatacga agagtttaac gagatccgcg
    15121 aacagctggc ggcgatcatc gaagaacaac ttgccgtgta caaaaccaga caagtgccgc
    15181 tggatcttgg tctggtggta cgcgaatatc tgtcacagta tccgcgtgca cgtcactttg
    15241 acgttgcgcg tattgttatt gatcaggcgg tacgtcttgg cgtagcgcaa gcagatttca
    15301 ccggactgcc agcgaaatgg cagccgatta atgattacgg agccaaggta caggcgcatg
    15361 tcatcgacaa atattgaaca agtgatgccg gttaagctgg cgcaggcgct ggcgaatccg
    15421 ttatttccgg cgctggacag cgccttacgt tcaggccgcc atattggcct cgacgaactg
    15481 gataatcatg cattcctgat ggattttcag gaatatctgg aagagtttta cgcgcgttat
    15541 aacgttgagc ttattcgcgc accggaaggg ttcttctatt tacgcccacg ttccaccact
    15601 cttatcccgc gttccgtctt gtcggaactg gatatgatgg tcgggaaaat cctctgttat
    15661 ctctatctca gcccggaacg gctggcgaat gaggggattt tcacccagca ggaactgtac
    15721 gacgaactgc tcaccctggc cgatgaagcg aaactgctga aactggtgaa caaccgttca
    15781 accggttcag acgttgaccg tcagaagttg caggagaaag tacgttcttc gctcaaccgt
    15841 ctgcgtcgtt taggcatggt gtggtttatg ggccacgaca gcagcaagtt ccgcattacc
    15901 gaatcggtgt tccgcttcgg agccgatgtg cgtgctggcg acgatccccg tgaagcacag
    15961 cgtcgcctga ttcgtgatgg cgaagcaatg ccgattgaaa atcatctgca actcaacgat
    16021 gaaaccgaag agagtcagcc agatagcgga gaggaagaat aatgattgaa cgcggtaaat
    16081 ttcgctcact gacgctgatt aactggaacg gcttttttgc ccgaactttt gaccttgacg
    16141 agctggtcac gacgctttcc ggcggtaacg gggcgggtaa atccaccacg atggcggcgt
    16201 tcgttacggc gctgatcccc gacctgaccc tgctgcattt ccgtaacact acggaagccg
    16261 gggccaccag cggttcgcgc gataaaggtc tgcacggtaa gctgaaagcg ggtgtctgtt
    16321 attcgatgct cgacaccatt aactcgcgcc accagcgcgt ggtggtcggt gtgcgtctgc
    16381 aacaggttgc cggacgcgat cgtaaagtgg atatcaaacc atttgccatt cagggactgc
    16441 cgatgtcggt gcagccgaca cagctggtga ccgaaaccct gaacgaacgc caggcgcgcg
    16501 tgctgccgct taacgagctg aaagacaagc tcgaggcgat ggaaggcgtg cagtttaaac
    16561 agttcaactc cattaccgat taccactcgc tgatgttcga tctgggcatc atcgcgcgtc
    16621 gtctgcgctc cgcatctgac cgtagcaaat tctatcgtct gatcgaagct tcgctgtatg
    16681 gcgggatctc cagtgccatt acccgttctc tgcgcgacta cctgttgccg gaaaacagcg
    16741 gcgtgcgtaa agcgttccag gacatggaag cggcgctgcg tgaaaaccgc atgacgctgg
    16801 aagcgattcg tgtcacccag tcggaccgtg acctgtttaa gcatctgatc agcgaagcta
    16861 ccaactacgt ggcggcggac tacatgcgtc acgccaacga gcgccgtgtc catctcgaca
    16921 aagccctgga gtttcgtcgc gagctacata cttcgcgtca gcaactggcg gctgagcagt
    16981 acaaacacgt cgatatggcg cgtgagctgg cagagcacaa cggtgccgaa ggtgatctgg
    17041 aagcggatta tcaggcggcc agcgatcacc tgaacctggt gcaaaccgca ttgcgtcagc
    17101 aggagaagat cgaacgctac gaagtggatc tcgatgagct gcagatccgt ctggaagagc
    17161 aaaatgaagt ggtggcagaa gccatcgaac gccaggaaga gaatgaggct cgcgcggaag
    17221 ctgccgaact ggaagtggac gagctgaaaa gccagcttgc tgactaccag caggcgctgg
    17281 acgtccagca aacgcgcgcg atccagtata accaggcgat tgctgcgctt aatcgtgcca
    17341 aagaactgtg ccatctgccg gacttaaccg ccgacagcgc cgccgaatgg ctggaaacct
    17401 tccaggcgaa agagctggaa gcgactgaaa aaatgctctc tcttgagcag aaaatgagca
    17461 tggcgcaaac cgcgcacagc cagtttgagc aggcttatca gctggtggtg gcaatcaacg
    17521 gcccgctggc gcgtaacgag gcgtgggatg tcgctcgcga actattgcgc gaaggggttg
    17581 atcagcgtca cctggcagag caggttcagc cgttgcggat gcgcttaagc gaactggaac
    17641 agcgtctgcg tgagcagcaa gaagctgagc gtctgctggc agatttctgc aaacgtcagg
    17701 gcaagaattt tgatattgat gaactggaag ctctgcatca ggagctggaa gcacgcattg
    17761 cctctctttc cgatagcgta tctaacgccc gtgaagagcg gatggcactg cggcaggaac
    17821 aggaacagct gcagtctcgc attcagagtt tgatgcagcg tgcaccggtt tggctggcag
    17881 cgcaaaacag tctcaaccag ttgagtgaac agtgcggcga agagttctcc tccagccagg
    17941 acgtcactga atatctgcaa cagttgctgg agcgtgagcg cgaggcgatt gttgaacgcg
    18001 atgaagtagg cgcgcgcaaa aacgccgtcg atgaagagat cgaacgttta agccagcctg
    18061 gcggctctga agatcagcgt ctgaacgcgc tggcggagcg ttttggtggt gtgctgctgt
    18121 cagaaattta tgacgacgtt agcctggaag atgcgccgta cttctcggct ctgtatggcc
    18181 cgtcacgcca cgccatcgtg gtgccagatc tgtcacaggt aaccgaacac ctggaaggct
    18241 tgaccgattg cccggaagat ctctatttga tcgaagggga tccgcagtca ttcgatgata
    18301 gcgtgttcag cgttgatgag ctggaaaaag cggtggtggt gaaaatcgcc gatcgacagt
    18361 ggcgttattc acgtttcccg gaagtgccgc tgtttggtcg tgctgcgcgt gaaagccgta
    18421 ttgaaagcct ccatgccgag cgtgaagtgc tttccgaacg cttcgccacg ctctcctttg
    18481 atgtacagaa aacccagcgt ctgcatcagg cgttcagccg ctttatcggc agtcatctgg
    18541 cggtggcgtt tgagtctgac ccggaagcag aaatccgtca actgaacagc cgtcgagtcg
    18601 aactggagcg ggcgttgagt aatcatgaaa atgataacca gcagcagcgt attcagtttg
    18661 agcaggcgaa agaaggcgtt acggcgctga accgcattct gccgcgtctc aacctgttgg
    18721 ctgatgacag cctggcggat cgcgtcgatg aaatccgcga acgtctggat gaagcccagg
    18781 aagccgcgcg ttttgttcag cagtttggca atcaactggc gaaactggaa ccgatcgttt
    18841 cggtattgca gagcgacccg gaacagttcg aacagttaaa agaagattac gcgtactctc
    18901 agcagatgca gcgcgatgcc cgtcagcaag cgtttgccct gacggaagtg gtgcagcgtc
    18961 gtgcgcactt tagctattct gactcggcag aaatgcttag cggtaacagc gatctcaacg
    19021 aaaaactgcg tgaacgtctg gaacaggcgg aagcggagcg tacccgcgct cgcgaagcgt
    19081 tgcgcggtca cgcagcgcag ttgagtcagt acaaccaggt gctggcttcg ctgaaaagtt
    19141 cttacgacac caaaaaagag ctactcaacg atctgcaacg tgaattgcag gatatcggcg
    19201 tgcgtgctga tagcggggca gaagagcggg cgcgtattcg ccgtgacgag ctgcatgcgc
    19261 aactgagcaa taaccgttca cgccgcaatc aactggaaaa agcgcttacc ttctgcgaag
    19321 cggagatgga caacctgacc cgcaaactgc gcaagctgga gcgggattac tttgagatgc
    19381 gcgagcaggt agtgaccgcc aaagcgggct ggtgtgcggt gatgcgcatg gtgaaagata
    19441 acggcgttga gcgccgctta caccgtcgtg agctggctta tctctccgct gatgatttgc
    19501 gttccatgtc ggataaggcg ttaggtgcgc tgcgtctggc ggtggcggat aacgaacatc
    19561 tgcgcgacgt gctgcgcatg tcggaagatc cgaaacgtcc ggagcgtaaa attcagttct
    19621 tcgtggcggt ttatcagcat ctgcgtgaac gtattcgtca ggatattatt cgtaccgatg
    19681 atccggtgga agctatcgaa cagatggaga ttgaacttag ccgtctgacc gaagaattaa
    19741 cctcccgtga acagaaactg gcgatcagtt cccgcagcgt ggcgaacatc attcgcaaaa
    19801 ccattcagcg cgagcagaac cgtatccgta tgctcaacca ggggttgcag aacgtatcgt
    19861 ttggtcaggt gaacagcgtg cgtctcaacg tgaacgtgcg tgaaacgcac gccatgctgc
    19921 tggatgtgct ctctgaacag cacgagcagc atcaggatct gtttaacagc aaccgtctga
    19981 ccttctcgga agcgttggcg aaattgtatc agcgtcttaa tccgcagatt gatatggggc
    20041 agcgcacgcc gcagaccatt ggcgaagaac tgctggatta ccgcaactat ctggaaatgg
    20101 aagttgaggt taaccgtggt tccgatggtt ggttgcgcgc agagtctggt gcattgtcga
    20161 ccggtgaggc gattggtacc ggtatgtcga ttctggtgat ggtggtacaa agctgggaag
    20221 atgaatctcg ccgcctgcgc ggtaaagata tctctccttg ccgcctgctg ttcctcgatg
    20281 aagcagcgcg actggatgct cgttctatcg ccacgctgtt tgaattgtgt gaacgtttgc
    20341 aaatgcagct catcatcgca gcgccggaaa atatcagtcc ggaaaaaggc accacctata
    20401 aactggtacg taaagtcttc cagaataccg aacatgttca cgtcgtcggc ctgcgaggat
    20461 ttgcgccgca actccctgaa acgcttctag gaagagacga agcgccttcc caggcgagtt
    20521 aaaattaagg cggcagcaat gccgcctttt ctttttccga aaactccgtt tctgcactaa
    20581 aaaaatggca cataacggcg taatgccgat cagttaagga tc
//
LOCUS       DAJTYH010000073        20505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTYH010000073 DAJTYH010000000
VERSION     DAJTYH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(97..1395)
                     /gene="eno"
                     /locus_tag="OO609_002647"
     CDS             complement(97..1395)
                     /gene="eno"
                     /locus_tag="OO609_002647"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6634013.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1483..3120)
                     /gene="pyrG"
                     /locus_tag="OO609_002648"
     CDS             complement(1483..3120)
                     /gene="pyrG"
                     /locus_tag="OO609_002648"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6634014.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3348..4139)
                     /gene="mazG"
                     /locus_tag="OO609_002649"
     CDS             complement(3348..4139)
                     /gene="mazG"
                     /locus_tag="OO609_002649"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6634015.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4235..6469)
                     /locus_tag="OO609_002650"
     CDS             complement(4235..6469)
                     /locus_tag="OO609_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6634016.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6517..7818)
                     /gene="rlmD"
                     /locus_tag="OO609_002651"
     CDS             complement(6517..7818)
                     /gene="rlmD"
                     /locus_tag="OO609_002651"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6634017.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            7875..10631
                     /gene="barA"
                     /locus_tag="OO609_002652"
     CDS             7875..10631
                     /gene="barA"
                     /locus_tag="OO609_002652"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6634018.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(10863..12203)
                     /gene="gudD"
                     /locus_tag="OO609_002653"
     CDS             complement(10863..12203)
                     /gene="gudD"
                     /locus_tag="OO609_002653"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6634019.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12224..13564)
                     /locus_tag="OO609_002654"
     CDS             complement(12224..13564)
                     /locus_tag="OO609_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6634020.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(13566..14918)
                     /gene="gudP"
                     /locus_tag="OO609_002655"
     CDS             complement(13566..14918)
                     /gene="gudP"
                     /locus_tag="OO609_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6634021.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15352..15801)
                     /locus_tag="OO609_002656"
     CDS             complement(15352..15801)
                     /locus_tag="OO609_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6634022.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(15819..16601)
                     /gene="truC"
                     /locus_tag="OO609_002657"
     CDS             complement(15819..16601)
                     /gene="truC"
                     /locus_tag="OO609_002657"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6634023.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16601..16930)
                     /locus_tag="OO609_002658"
     CDS             complement(16601..16930)
                     /locus_tag="OO609_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6634024.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(17552..18097)
                     /gene="syd"
                     /locus_tag="OO609_002659"
     CDS             complement(17552..18097)
                     /gene="syd"
                     /locus_tag="OO609_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6634025.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18165..19013
                     /gene="queF"
                     /locus_tag="OO609_002660"
     CDS             18165..19013
                     /gene="queF"
                     /locus_tag="OO609_002660"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6634026.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDKDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19125..20489
                     /gene="ppnN"
                     /locus_tag="OO609_002661"
                     /gene_synonym="ygdH"
     CDS             19125..20489
                     /gene="ppnN"
                     /locus_tag="OO609_002661"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6634027.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5007 a         5519 c         4932 g         5047 t
ORIGIN      
        1 tggaggctca agtggcggcg catccggccg ctggtaactc caccataaaa aatgccagcc
       61 cgaaggctgg cattatcaaa tcagataaag tcagacttat gcctggcctt tgatctcttt
      121 acgaccgttg tacggtgctt tttcgcccag agcttcttcg atacgaatca gctggttgta
      181 tttagcaaca cggtcagaac ggctcataga accagttttg atctggcctg cagcagtacc
      241 aacagccaga tcagcgatgg tagcatcttc agtttcacca gaacggtgag agataactgc
      301 agtgtagcca gcatctttcg ccatcttgat tgcagccaga gtttcggtca gagaaccgat
      361 ctggttgaat ttgatcagga tggagttagc gatacctttt tcgatacctt ctttcaggat
      421 cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt
      481 ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat
      541 cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt
      601 gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc
      661 catcgccaga gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat
      721 aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac
      781 agcagtgttc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc
      841 catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat
      901 atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat
      961 ggagtatttg cccggagtgc cgttcagttc agcgatgtgc tcgtacagcg gcataccttt
     1021 agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa
     1081 tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc
     1141 tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt
     1201 ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg
     1261 ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg
     1321 tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt
     1381 tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac
     1441 aacgcgtacc caaggtacgc gttgccgttc taactttttt acttacttcg cctgacgttt
     1501 ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt
     1561 agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat
     1621 gatctcgacc aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat
     1681 ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg
     1741 cgcattgtat agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag
     1801 acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc
     1861 gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attctgtaga
     1921 gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc
     1981 caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat
     2041 gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag
     2101 gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac
     2161 acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag
     2221 ttcaatgtac ttgccgacca taccgatggt gacttcactt accggattcg cttcttcgaa
     2281 gataacctgt tcccattcgg acagatttgc ttccgggcag tttaagctga atcgtttaca
     2341 aatataatcg tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac
     2401 gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg
     2461 ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat
     2521 ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc
     2581 catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc
     2641 catctggcga atagcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat
     2701 ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt
     2761 agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg
     2821 cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg
     2881 aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca gcgccgtctt cagtaacgaa
     2941 cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt aaggatccag
     3001 tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc
     3061 aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat
     3121 gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaagacggg aaagcagtat
     3181 acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt
     3241 gaaatcagga gtgagaaaat agcccctttg gggtaaatgt ttttgacgca aatcaagcgc
     3301 ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt aattccctta gagatcaatt
     3361 tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt
     3421 tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg
     3481 ttcacttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg
     3541 gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggtcgacaac agcctgccgc
     3601 gcctcgtaca tcacctcgtc gatctcttcg tagactttat caaccaccgg gccaagcgtc
     3661 gtccagtcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcccg cattaaagcc
     3721 ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg
     3781 gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca
     3841 aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag
     3901 tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg
     3961 cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag
     4021 gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag
     4081 ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacggtcgat ttgattcatt
     4141 gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc ttcgagtaaa agatatcgct
     4201 ggatacggct gacgccatat ccagctatgt aagactaact cccgtgcaac cgacgcgcgt
     4261 cgataacatc cggcacctgg ttgagtttac ccagcacgcg ccccagcact tgcaggttgt
     4321 aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt ggtgtcgcta cggctggcaa
     4381 cgccaagcac gttcaccttc tcgttggcga gaatggtcgt gatatcacgt aacaacccac
     4441 tacgatcatt agccaccacg cggaccacca gcgaatatcc ggcggagtag ctctcacccc
     4501 ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag ttccgccagt tgttcgcaat
     4561 cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa gccgacaatc tcatctccag
     4621 gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt accaacacct tcaaccacca
     4681 cacgaccgtt atctttactg cggttttgcg gtgtgtagct tttttgctga agctgtttca
     4741 gcgcggcggc gtcctgctct tcggcactcg gcttattaaa ttgcgattgc aggaagttca
     4801 ccatctgatt gagacggata tccccgccac caatcgccgc cagcaactcg tcgacatcat
     4861 tgaagttgta acgcggcagc agatgttttt ctgcttcttt caggctgatc cccagatgtt
     4921 ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt tttgtcacgg tcctgtttac
     4981 ggaaccaggc gtgaattttc gaacgcccac ggctggttgt gacgtaaccg aggtttgggt
     5041 ttaaccagtc acggctgggg ttcggctgtt tctgggtgat aatttcaatc tggtcgccca
     5101 tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat ttttgccccg atgcagcggt
     5161 gtccgacatc actgtggatg tggtaagcga agtccagcgg cgttgatccc gcaggcaaat
     5221 caacgacatc acctttcggc gtaaagacat acacccggtc gtcaaacacc tgactacgta
     5281 cttcgtcgag catttcgccg gaatcagcca tctcttcctg ccacgcaatc agtttacgca
     5341 gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc agctgccgcg ccctctttat
     5401 atttccagtg cgcagcaaca cccaactccg catcttcatg catctgtttg gtgcggattt
     5461 ggatctcaac ggtttttcca cccggcccca gaaccacggt atgaatagac tgataaccgt
     5521 ttggtttcgg gttagcgacg taatcgtcaa actcatccgg caggtggcga tagtgagtgt
     5581 gcactatccc cagtgcggca taacaatcct gtaaacgctc ggcgacaata cgtaccgcac
     5641 gcacatcaaa cagctcatcg aaggcgaggt tctttttctg cattttgcgc cagatgctgt
     5701 agatgtgttt cggacgacca tacacttcag ctttaacgcc ttcagctttc atctcagcgc
     5761 gcagatggcc gacaaactct tcgatatagt gttcgcggtc gagacggcgt tcatgcagca
     5821 gttttgcgat gcgtttgtat tcggtcgggt ggagataacg gaagcagtaa tcttccagct
     5881 cccatttcag ttgcccaatc ccaagacggt tcgccaacgg cgcgtagata ttggtgcact
     5941 cttttgccgc cagtacacgt tcatcttccg gcgcatcttt tacttcgcgc agatgagcaa
     6001 tacgctccgc cagtttgatg actacgcagc gaaaatcatc gaccatcgcc aataacatcc
     6061 ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt gtgcgtcgct ttcagctggc
     6121 ggatcgccgc catatcacgc acgccgtgaa taaggttaac aaccgactta ccgacgctct
     6181 cacgcagcac atcttcgctg actacgttgg catcagccag agggaacagc agcgccgccc
     6241 gcagcgtgtc aatgtccata cttaatgtcg agaggatctc caccatctca acaccacgcc
     6301 acaataacag actggcatcc ggatgcccct gcgtctgttg cagacaatac gcccaggttt
     6361 cggctaagca ctcacacgac ttctggctgg taatacccag acttgcgatc catttttccg
     6421 gatcaaattc accagcctta ttgatatgtg cacttcttac cgcaaccatc gtcctctcct
     6481 ttagggacca gacctgccga aatcggcaaa tcgtaactat ttaacgcgcg agaaaagtac
     6541 catcgattcc agatgtcccg tgtgtgggaa catatccagc atcgccagtc gcgcaatggt
     6601 atatcctgct tttaataacg cttcgctatc ccgagccagc gttgcagggt tacaggatac
     6661 ataaactata cgaataggtt ccagttttat aatttgctgc ataacacctg cggcacctgc
     6721 tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc gcccacggct gctttgtgac
     6781 atcttcttca agattttcgt gataaaacgt cacattctgt aagccattaa gacgcgcatt
     6841 ctgctggcct ttttccacca gcgcaggaac accttctaca ccgaccacac tggcagcttg
     6901 tgtcgccaat ggcagtgtaa agttgcccat accgcagaac agatccagta cgcgatcttc
     6961 aggttgcaca tccagccatt ccaacgcacg cgctaccatt ttttggttca cacccgcatt
     7021 gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac ccgtttgagt cataccaggg
     7081 catctcacca gagacggttt cgagtatctc actatcgggg gcgagataca gatccaggcc
     7141 ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc gaacttagcg gtgcggtatg
     7201 gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt tcaacatgac caagatggcg
     7261 catagcttgt aagctgccca gacatgccct gactttgggc agcaatgctt caagttgggg
     7321 cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg gagcccgctt tgcgaaaccc
     7381 catctgaagt tgctgtgttt tcggtaagta gttcagactt aaacgcgcgc ggcggcgata
     7441 gccccaggga acatcggcga tcacttcaga gacatcgtgt ttcattaatc gggcgagtgc
     7501 cgcacttttg cttcgctgct gtaaatccac gctggcgtgt tgttgctgac agccgccgca
     7561 tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg ctatcgctta accggcgtac
     7621 gactttagcg cgggcatact gttttttatc ttcagtaaca gcaacttccg cgttttcctg
     7681 cggcaataat ccggggataa ataacgtttt gccgttatgt cgcgccacgc cctgaccaaa
     7741 agagtcgagg tcgttgactg aaacggttat gatctgacgc gtcgtcgtgc gtcgttttgc
     7801 agagtagaat tgcgccattg gcgagacttt ctcaatttaa cagtgtgacc ttaattgtcc
     7861 cataacggaa ctccatgacc aactacagcc tgcgcgcacg catgatgatt ctgatcctgg
     7921 caccgaccgt ccttattggt ttattgctga gtatcttttt cgtcgtgcat cgctataacg
     7981 acttgcagcg tcaactggaa gatgccggtg ctagcattat tgagccgctt gcagtttcta
     8041 ctgaatatgg catgagcctg caaaatcgcg aatctatcgg tcagttaata agcgtactgc
     8101 atcgtcgcca ttccgatatt gttcgcgcga tttcggttta tgatgaaaat aaccgactct
     8161 ttgtcacctc caattttcat cttgatccct catcaatgca gctcggcagc aacgtgccgt
     8221 ttcctcgcca gctcactgtc actcgtgacg gcgatattat gatcctccgc acgccgatta
     8281 tttctgagag ttactccccc gacgaatcgc ccagtagcga tgccaaaaat agtcaaaata
     8341 tgttgggata tattgcgctt gagctggatc ttaaatcggt tcgcttgcag caatataaag
     8401 agatctttat ttcctgcgtg atgatgctgt tttgtatcgg tattgcgctt atttttggct
     8461 ggcgcttaat gcgcgatgta accggtccga ttcgcaacat ggtgaatacc gtcgaccgca
     8521 tccgtcgcgg gcaactcgac agccgggtgg aaggatttat gctcggcgag ctggatatgc
     8581 tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc ttatcacgaa gagatgcagc
     8641 acaatatcga ccaggcgacg tccgatctgc gtgaaacgct ggagcagatg gaaattcaga
     8701 acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc ggcgcgtatt aaatccgagt
     8761 ttctggcaaa tatgtcacac gagctgcgta caccactgaa tggtgttatt ggctttaccc
     8821 gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga tcacctgaat acgattgaac
     8881 gttcggcaaa taatttgctg gcaattatta atgatgttct cgacttctcg aaactggaag
     8941 caggtaagct gattctggaa agtattccat tcccactacg cagcacgctg gatgaagtcg
     9001 ttactctgct ggcacattct tctcacgata aagggttgga actgacgctc aatattaaaa
     9061 gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt acagcaaatc atcactaacc
     9121 tggtggggaa tgcaattaaa ttcaccgaga atggcaacat tgatattctg gtagaaaaac
     9181 gtgcgctgag taataccaaa gtgcagattg aagtgcagat tcgggatacc ggcattggta
     9241 ttcctgaacg cgatcaatcg cgcttattcc aggccttccg acaggctgat gccagtattt
     9301 cccgccgtca tggtggcacc gggctggggc tggtgattac acaaaaactg gttaatgaaa
     9361 tgggcggcga tatttcgttc catagccagc cgaatcgcgg ttcaactttc tggttccaca
     9421 ttaatctcga tctgaacccg aacattatta tcgaagggcc atccatccag tgcctcgcag
     9481 gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc gcaatgcacg ctggatattt
     9541 taagtgaaac gccgctggaa gtggtttata gcccaacgtt ctccgcgctg cctcccgcgc
     9601 attacgacat gatgttgtta ggcatcgcgg tgaccttccg cgagccgcta acaatgcaac
     9661 atgagcgatt agcgaaagcg gtatcgatga ccgatttcct gatgctggca cttccttgcc
     9721 atgcacaagt taatgctgaa aaactcaagc aagatggtat cggcgcgtgt ctgctgaaac
     9781 cattaacacc tacgcgcctg ttgcctgccc tgacggaatt ttgtcatcac aaacaaaaca
     9841 cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac agtcatggcg gttgatgaca
     9901 accccgctaa cctgaaactt atcggcgcat tgctggaaga tatggtgcaa catgtggaac
     9961 tttgcgatag cgggcatcag gcagttgaac gggcgaaaca gatgccgttc gatttgatct
    10021 taatggatat tcaaatgccg gacatggatg gcattcgggc ctgcgaactc atccaccaac
    10081 tcccgcatca gcgacaaacg ccggttatcg cggtaacggc gcatgcaatg gccgggcaaa
    10141 aagagaagct gcttggcgca gggatgagcg attatctggc gaaaccgatt gaagaagagc
    10201 gattgcataa tttgttgttg cgctacaagc ctggcagcgg tatttcgtct cgcgtcgtga
    10261 cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac cctcgactgg caactggcac
    10321 tacgccaggc agcaggaaaa accgatttag cgcgcgatat gctgcaaatg ttactcgatt
    10381 tcctgcctga agttcgcaac aaagttgagg aacagctggc tggagaaaac ccggaaggcc
    10441 tggttgattt gattcataaa ctgcatggca gttgcggcta tagcggcgta ccacgtatga
    10501 agaatctctg ccagctgatc gaacaacagc tacgtagcgg tactaaagaa gaagatttgg
    10561 agccggagct gctggaactg ttggacgaga tggataatgt cgcgcgcgaa gccagcaaaa
    10621 ttctcgggta acggacgtct tgtcgagtag caatcggctt tttatgacgc tggtttcaga
    10681 ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt aacgccttat ccggcctaca
    10741 aaaccaatta aattcaatga attaaaaaat gatgtagacc tgatacatag cacaacaggc
    10801 tattttgcgt ttagcatcag tctcaaaccg gctccagata gagccggttt tggttttctg
    10861 tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc agccaggaat cagatactgc
    10921 attcccatcg catcgtcacg cgcgccaagc ccgtgtttct gatacagctc atgggctttc
    10981 atcacttgat ccatatcgat ttctacaccc agccccggtt tttctggcac ctgtaccagc
    11041 ccgcctttga tctcaaacgg ttctttggtc aggcgctgat taccttcctg ccagatccag
    11101 tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg caacatgggt aaacatcgcc
    11161 agggaaatat cgaagtggtt gtttgagtgt gaaccccagg tcaggccaaa ttcatggcac
    11221 atttgcgcca cacgtaccga accttgcata gtccagaaat gcggatccgc cagcgggata
    11281 tcaacggatt gtaacgagag cgtatggccc atctgccgcc agtcggtggc gatcatattg
    11341 gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca tcacttcacg cccggagaaa
    11401 ccttgctccg caccacacgg atcttctgca taagccagcg aacctttcag gtatttacca
    11461 attttaattg cttcgttcag cgaccaggca ccgttaggat cgagcgtaat acgcgcctgc
    11521 gggaagcgtt gcgccagtgc cacaatagac tcggcttctt cttccccggc cagtacaccg
    11581 cctttcagtt tgaaatcgtt gaagccatat ttttcatacg ccgcttccgc caggcgcacc
    11641 accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat accagtcgca tgagtcatcc
    11701 ggctggcttt gatacggcag aggtgtggct ttgcgattac cgacgaagaa cagataaccg
    11761 agcatttcga cttcgctacg ctgttgacca tcgcccagca gcgatgccac gtttaccccc
    11821 agatgctgcc ccagcagatc cagcattgcc gcttctatcc cggtaactac atgaatagtg
    11881 gtacgcaaat caaatgtctg caaaccgcgc ccactagcat cacgatcggc aaaagtatta
    11941 cgcaccagcg tcagaacgtt tttgtattca cccagcgttt tacctaccac cagcggaatc
    12001 gcatcttcca gcgttttacg gattttctcg ccgccgggaa tttcccctac gccagtgtga
    12061 ccggaattat ctttgataat cacaatatta cgcgtgaaga acggtgcgtg tgcaccactc
    12121 agattcatca gcatactgtc atgacccgcc accgggatga cctgcatttc agtaacaaca
    12181 ggcgtcgtaa attgagaact catatttatg tcctttttca gaatcaatga cggccgaaaa
    12241 cgggacgttt acggtcaaag gtccagccgg ggatcaggta ctgcatcgga cctgcgtcgt
    12301 tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc cttttgtacc tgttcccagt
    12361 ccagttccac gcccagaccg ggcgcatcag gaacggcaat ttttccgttt ttaatctcca
    12421 gcggattttt ggtcaggcga caatcgccct cctgccaaat ccagtgggta tcgatagcgg
    12481 taggattacc cggtgccgcc gcgcccacat gggtaaatat cgccagagag atatcgaaat
    12541 ggttgttgga atggcagccc caggtcagcc cccagtcgtc gcaaagctgc gccacgcgga
    12601 ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg aatatctacc gcattgagca
    12661 tcaccgcatg acccatttcg cgccagttgg tggcgatcat gttagtcgcg acgggcaagc
    12721 cggtcgcccg tcgaaactcc gccatcactt cacgtccgga gaagccctgt tctgcgccgc
    12781 atggatcttc ggcataggta agaacatcat tcagcccttt gcataaagaa atggcttcat
    12841 caagcagcca tgcaccgttg ggatcaacgg taatccgcgc atccgggaag cttttcttca
    12901 atgcacgaac agtgtcgatt tcttgctcgc caggtaacac cccgccctta agtttgaaat
    12961 ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg cacaacggct tcgctgttca
    13021 tcgctttctg atggcgcaac tgataccact catggttgcc cggcgtattt tccagataag
    13081 gaagatcggt tttggtccga tcacctatat aaaacagata accgaggacg gtaatagtct
    13141 cgcgttgctt gcctggcccc aacagttcgc agaccggaac attcagcgcc ttacctagca
    13201 ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac gcgcaattca aaagtccagg
    13261 cacctttgcc aaaggtatca aaatcggctg cctgattacc tttatgtacc tgctgaacca
    13321 ctttattcag gcgcgcaact tcctggccca gaaccatcgg aatagcatcg accagtgttt
    13381 gataaatcac ctctccgccc ggcgcttcac caatgccggt atgcccggcg ttatcggtga
    13441 gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc accaatattg agcaacatgc
    13501 tgtcatgtcc ggcgaccgga atgactttca tatcagtaat aacaggactg gattgtgtcg
    13561 ccatcttatt gccccgcgac aggtttcagc tcgatacgct tgatatcgcc caccagcacc
    13621 aggtagctca gtaccgcgat tagggcatga acaccaacat aaatcagcgc cccattaaac
    13681 gagccagtcg tgccaacgat ataaccaatt gcgattggcg tgacgatgcc agaaatgtta
    13741 ccgaacatgt tgaacaggcc accggaaaga ccgctgatct cttttggcgc ggtatctgcc
    13801 attactgccc aacccagcgc cccgatgccc ttaccgaaga aggccagcgc cataaagccg
    13861 atgatcatcc actcaacgtt gacgtagttg cagaacacca tcaccatcga cagcaacatg
    13921 cccattacga tcggtgtttt acgcgcaatg ttcagcgatc ccgtgcggcg catcagccag
    13981 tcggaaataa ttccacccag cacaccgccg ataaaaccgc aaaccgccgg aacggaagcc
    14041 acaaagcccg ctttcagaat cgacatccca cgcgcctgca ccagataaac cggaaaccag
    14101 gtaatgaaaa agtaagtcag ggcgttgata cagtactgac cgatataaac gccgatcatc
    14161 atccgtgacc cgagcagctg tttgatctgc ccccacttca cgctgaacgg cactttaact
    14221 ttggtgcttt gctgatccat attgatcagc gcgccacccg cggcgatata ctccagctct
    14281 ttctgattta cccccggatg ttgatttggc tcgtggatga ctttcaacca gataaagctg
    14341 atgacaatgc ccagaccgcc cataaagaag aagacgtgtg accagcccac ttcatgcgtc
    14401 agccagccca taatcggcgc gaagatcacc gttgcgaagt attgagcgga gttaaaaatc
    14461 gacaccgccg ttcccctttc ctgcaccgga aaccaggccg caacaatgcg actgttgccg
    14521 gggaaagatg gcgcttcagc aagcccgacc aggaagcgca gagtaaacag ggcaacgata
    14581 atgccgaatc cactaaagat atcgacgaag ccttgcagca aggtaaacat cgaccagata
    14641 aagatcgacc agaagtagac gcgttttgaa ccaaaacgat ccagcaacca accaccaggg
    14701 atctgcccga taacataagc ccatgagaag gcagagaaca catagcccat tcccacggga
    14761 tcaaggccga tatctttggc catttccgaa ccggcaatag aaagcgtagc gcggtcgccg
    14821 tagttgaagg atgtgacgat aaacaacatc accactatcc agtaacgagc attcgtgcgt
    14881 ttttccacac tgctcgcagc ctgacttaaa gaactcattg ttgcactcct gaaaattcgc
    14941 gttagccacg ctcactctgg actgcgacat cgccaggaaa tcagaggtga cgtagggtgt
    15001 tttttgccgt ttttataggt cgttcgccga atacggcgcg tgtttatatc tggcaatagc
    15061 agtataaaaa gcgcgccata gcggctcacc gtgcaacaac acaacattaa tgcgttcaat
    15121 gaggcccgat tttggcatta gccctggacg gtggaatcca cttcacggaa atgaaaacaa
    15181 gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga gtatgaaatg gatcgcttga
    15241 ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag tttttactgg catttgcctg
    15301 aaaagattcg attcagcacc gctaaaacga catttaccgc tcgctgacat atcaggacaa
    15361 cagcgtgccc cactgttcga cccacggatt tgattccgtt tccggttccg ggttctcact
    15421 ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc tgttcctgca acaaggcatc
    15481 gaactgtttg ccgccattgc aaaaatttac ataactgctg tcgccgagcg caatcacgcc
    15541 ataacgcaga ttcggctgga agcccagact atctttgatt ccctgaaaga gcggcacaat
    15601 gctatcagga aggtcgccct gcccggtcgt ggacgtaacc accagaacat acttatcctg
    15661 atagggcagc cagtcgctta attcaggatc ttcaaatacc gttgctttgt ggccctgtgc
    15721 ggtcagaatt gcttctgctt cttccgccac taacagcgaa tttccgtaca tggtgccgac
    15781 aaaaatacca atttccgcca taccgttttc tccctggatt aggaacttat ctctccatcc
    15841 tgacccgatg gcgcactgaa ctcaaccctt tcattttcag gaagcagacc gcgccagcca
    15901 aattgtgata acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg
    15961 ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta gcattaaccg ctggaggcca
    16021 aaatgctcag caccgctgcg attctggcgt aaatcgccat gtttgctatc gccaataatc
    16081 ggatgacgca aatgggcaag atgtcgtcga agctgatgtt tgcgtccggt tttcggctcc
    16141 agttccacca ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg catttctacg
    16201 gtcgccagac cgcgataatg cgtcactgct ggctgcgggc ctttatcttc gcgggcaaat
    16261 ttatcagcga ttttgtccag ttcttccacc agtggataat ccaacaccgc ttcttccatc
    16321 aaccagccgc gcacaatcgc atggtaacgt ttctggattt ggtgctgttc aaactgttgt
    16381 gccagcagcc gtccggcctc gctggataat cccatcaaca acacaccaga agtgggtcgg
    16441 tccagacgat gagcagtaaa aacatgctgt cctatctggt cacgcacggt ttgcatgacc
    16501 actactttct cgtcgcgatc cagccagcta cggtgaacca gccagccgga gggtttattt
    16561 accgcaacca gccattcatc ctgatagagt atttccagca ttagctcgca tcatccgcaa
    16621 aaagagcatc cagtttttcc aactcagcca gaataagcgc gcgttgcgga tggtccgtcg
    16681 ccagcgccat ttcataatag ggtgcaacag caaaagcgcc cggtaacggc tggttgttat
    16741 ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa ccactccagt ggttccatgg
    16801 tgtccataaa gaacggttgg gtactattaa attgatgcgg ctggggttca tcgtttcgcc
    16861 agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat
    16921 gagtggtcat aaagcaacct caataagaaa aactgccgcg aaggatagca ggaataaaaa
    16981 aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc agcattccag ctacttttgt
    17041 tgctccctgc tcatccttga caacttttcc tctggccttg cggccaatcg ttcatcctga
    17101 actattgctt cctgctcaca ccaccccgat gtgatacttc atcctgaagt gtccctggcc
    17161 ttcctgaccc accgaatcat cctgaccggt tctcattctc catcctggag gtgtccttta
    17221 acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa caccatcctg
    17281 gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc gttgtctgac tccctgtcga
    17341 cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg cttacagaca atgccttaag
    17401 gaaaatttca tatgaaagtt aagaattaac aatcaacatt ttgattttaa atgaaaataa
    17461 cgattcttat ctttttaagt attcgcttat ttcctggcga tctcctacag agtctgtaag
    17521 agatctctca caaccagatg gggtatttgg attacagaag aggcttaagt tgattaagga
    17581 attccgcaag atttgaggct aaattcgtcc gtttacgtgt tcccagcgtc tctttgcaca
    17641 cttcaccgga gagattacac accgagatga cctctaactc ttcttccagc gtcgcgataa
    17701 atagcgtggg cggcagtttt agccttttct gagtcaccag atgaccaatc agattttcct
    17761 gcacccgacg gaagtcgtct tcactccagg tttgcagcaa tgtcagtttg atatcgccaa
    17821 actgcgcgtg catatccccg gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta
    17881 tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta aacggctggg
    17941 gttgccagta tacggcatct tcggtagttg aaataatgca cggtgaagga acaccgtaca
    18001 attcctcact taacggccag cttttatgct cttcatgcca cgcatcgcag tagcgtgcag
    18061 taaaatcttt cagggcttgt gcggtcaaat cgtccactaa tttctctctt cacttaaacc
    18121 agatacactt gtcgtttagt ttatctggtt tatgacggtg aaacatgtct tcttatgcaa
    18181 accatcaggc acttgcgggc ctgactcttg gaaaatcaac cgattaccgg gatacctatg
    18241 acgccagcct actgcaaggc gttccacgca gcctgaatcg cgacccgctg ggtctgaaag
    18301 cggataacct gccttttcac ggtacggata tctggacgct gtatgaactt tcctggctga
    18361 atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga acttgattac accagcgtaa
    18421 atctgattga gtcgaagagt tttaagctct atctcaacag ttttaaccag acgcgtttta
    18481 ataactggga tgaggtgcgc cagacgctgg agcgcgactt aagcacttgc gctcagggta
    18541 agattagcgt ggcgttatat cgtcttgatg aactggaagg ccagccgata ggtcatttca
    18601 atggcacttg cattgatgac aaggatatca ctatcgataa ctatgaattc actactgact
    18661 atctggagaa tgccaccagc ggtgaaaaag tagtggaaga gacgctcgtc agccacctgc
    18721 tgaaatcaaa ctgcctgatc acccatcaac cagattgggg ttcgatccaa attcagtatc
    18781 gtggacgcca aattgacaga gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca
    18841 acgagttcca cgaacagtgc gtggaacgca tctttaatga cctgttacgc ttctgccagc
    18901 cagagaaatt gagcgtttac gcacgttata cccgtcgtgg cggtctggac attaacccgt
    18961 ggcgcagtaa tagcgatttt gtcccatcga ccacaagact ggttcggcaa taaatttttt
    19021 ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt
    19081 gtaatcaaag ggaatgacga tattcgtccc ataaggagtt tttcttgatt acacatatta
    19141 gcccgcttgg ctccatggat atgttgtcgc agctggaagt ggatatgctt aaacgcaccg
    19201 ccagcagcga cctctatcaa ctgtttcgca actgttcact tgccgtactg aactccggta
    19261 gtttgaccga taacagcaaa gaattgctgt ctcgttttga aaatttcgat attaacgtcc
    19321 tgcgccgtga acgcggcgta aagctggaac tgattaatcc cccggaagag gcttttgtcg
    19381 atgggcgaat tattcgcgct ttgcaggcca acttgttcgc ggttctgcga gacattctct
    19441 tcgtttacgg gcaaatccat cataccgttc gttttcccaa cctgaatctc gacaactccg
    19501 tccacatcac taacctggtc ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg
    19561 aagcgccaaa tatggtggtc tgctggggcg gtcactcaat taacgaaaac gagtatttgt
    19621 atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct gaatatctgc accggctgtg
    19681 gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt
    19741 acaaagacag tcgttttatt ggtatgacag agccgtcgat tatcgccgct gaaccgccta
    19801 acccactggt caacgaattg atcatcatgc cggatatcga aaaacgtctg gaagcgtttg
    19861 tccgtatcgc tcacggcatc attatcttcc ctggcggtgt gggtacggca gaagagttgc
    19921 tgtatttgct gggaatttta atgaacccgg ccaacaaaga tcaggtttta ccattgatcc
    19981 tcaccggccc gaaagagagc gccgactact tccgcgtact ggacgagttt gtcgtacata
    20041 cgctgggcga aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc gctgaagtcg
    20101 ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgtgat acaggcgatg
    20161 cctacagctt taactggtca atgcgcattg cgccagattt gcaaatgccg tttgagccgt
    20221 ctcacgagaa tatggctaat ctgaagcttt acccggatca acctgttgaa gtgctggctg
    20281 ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa cgtaaaagaa gtcggtattc
    20341 gcgccattga agagtttggt ccttacaaaa tcaacggcga taaagagatt atgcgtcgta
    20401 tggacgacct gctacagggt tttgttgccc agcatcgtat gaagttgcca ggctcagcct
    20461 acatcccttg ctacgaaatc tgcacgtaat ctcccctccc ccggt
//
LOCUS       DAJTYH010000074        20504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTYH010000074 DAJTYH010000000
VERSION     DAJTYH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            28..327
                     /locus_tag="OO609_002662"
     CDS             28..327
                     /locus_tag="OO609_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6634028.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            337..861
                     /gene="ygaP"
                     /locus_tag="OO609_002663"
     CDS             337..861
                     /gene="ygaP"
                     /locus_tag="OO609_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6634029.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(908..1312)
                     /gene="stpA"
                     /locus_tag="OO609_002664"
     CDS             complement(908..1312)
                     /gene="stpA"
                     /locus_tag="OO609_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6634030.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            1980..2429
                     /gene="alaE"
                     /locus_tag="OO609_002665"
     CDS             1980..2429
                     /gene="alaE"
                     /locus_tag="OO609_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6634031.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2466..2810)
                     /locus_tag="OO609_002666"
     CDS             complement(2466..2810)
                     /locus_tag="OO609_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6634032.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            2962..3192
                     /locus_tag="OO609_002667"
     CDS             2962..3192
                     /locus_tag="OO609_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634033.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3539..3784
                     /gene="nrdH"
                     /locus_tag="OO609_002668"
     CDS             3539..3784
                     /gene="nrdH"
                     /locus_tag="OO609_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6634034.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3781..4191
                     /gene="nrdI"
                     /locus_tag="OO609_002669"
     CDS             3781..4191
                     /gene="nrdI"
                     /locus_tag="OO609_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6634035.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4164..6308
                     /gene="nrdE"
                     /locus_tag="OO609_002670"
     CDS             4164..6308
                     /gene="nrdE"
                     /locus_tag="OO609_002670"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6634036.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6318..7277
                     /gene="nrdF"
                     /locus_tag="OO609_002671"
     CDS             6318..7277
                     /gene="nrdF"
                     /locus_tag="OO609_002671"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6634037.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7632..8834
                     /gene="proV"
                     /locus_tag="OO609_002672"
     CDS             7632..8834
                     /gene="proV"
                     /locus_tag="OO609_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6634038.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8827..9891
                     /gene="proW"
                     /locus_tag="OO609_002673"
     CDS             8827..9891
                     /gene="proW"
                     /locus_tag="OO609_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6634039.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9949..10941
                     /gene="proX"
                     /locus_tag="OO609_002674"
     CDS             9949..10941
                     /gene="proX"
                     /locus_tag="OO609_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6634040.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11133..12312
                     /locus_tag="OO609_002675"
                     /pseudo
     CDS             11133..12312
                     /locus_tag="OO609_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12436..13173
                     /gene="ygaZ"
                     /locus_tag="OO609_002676"
     CDS             12436..13173
                     /gene="ygaZ"
                     /locus_tag="OO609_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6634041.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13163..13498
                     /gene="ygaH"
                     /locus_tag="OO609_002677"
     CDS             13163..13498
                     /gene="ygaH"
                     /locus_tag="OO609_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6634042.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13589..14119
                     /gene="emrR"
                     /locus_tag="OO609_002678"
     CDS             13589..14119
                     /gene="emrR"
                     /locus_tag="OO609_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6634043.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14246..15418
                     /gene="emrA"
                     /locus_tag="OO609_002679"
                     /pseudo
     CDS             14246..15418
                     /gene="emrA"
                     /locus_tag="OO609_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15435..16973
                     /gene="emrB"
                     /locus_tag="OO609_002680"
     CDS             15435..16973
                     /gene="emrB"
                     /locus_tag="OO609_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6634044.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17037..17552)
                     /gene="luxS"
                     /locus_tag="OO609_002681"
     CDS             complement(17037..17552)
                     /gene="luxS"
                     /locus_tag="OO609_002681"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6634045.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17702..19258)
                     /gene="gshA"
                     /locus_tag="OO609_002682"
     CDS             complement(17702..19258)
                     /gene="gshA"
                     /locus_tag="OO609_002682"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6634046.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19331..19759)
                     /locus_tag="OO609_002683"
     CDS             complement(19331..19759)
                     /locus_tag="OO609_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6634047.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19756..20322)
                     /gene="yqaB"
                     /locus_tag="OO609_002684"
     CDS             complement(19756..20322)
                     /gene="yqaB"
                     /locus_tag="OO609_002684"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6634048.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4954 a         5251 c         5122 g         5177 t
ORIGIN      
        1 cttaatttag aaaatactta aataaatatg actgaactcg cgcaattaca ggccagtacc
       61 gaacaggcag cggccttatt gaaagcaatg agccacccta aacggttgct gattctgtgc
      121 atgcttagcg gttcccccgg caccagcgcg ggagagctga cgcgcattac cggactgagc
      181 gcctctgcga catcacagca tctcgctcgt atgcgggacg aagggcttat caacagccaa
      241 cgggatgccc aacgcattct atattccatt aaaaatgagg cggtaaatgc cattatcgcc
      301 accctgaaaa atgtctattg tccgtaagga gtcactatgg ctttgacaac catttcgccg
      361 catgatgcac aagaattaat cgcacgcggc gcaaagttaa tcgatattcg tgacgctgat
      421 gaatatcttc gtgaacatat tcctgaagca gatctggctc cattatccgt gctggaacag
      481 tcaggtcttc cacctaaatt acgtcgcgag caaattatat tccactgcca ggcaggtaaa
      541 cgcaccagta ataacgccga taaattagca gcgattgccg cccccgcaga aatattttta
      601 ctcgaagatg ggattgatgg ctggaaaaga gctggattgc cagtagcggt aaataaatct
      661 caacccttgc cgttaatgcg ccaggtgcag atcgctgcgg gtggtttaat attaaacggc
      721 gttgtactgg gttataccgt aaatagcggt ttcttcttat taagtggctt tgttggtgcc
      781 gggttactgt ttgcaggaat cagcggtttt tgtggaatgg caaggttgtt agataagatg
      841 ccgtggaacc aacgagcttg agaagcgacg ccggacgcgc cctagcagcg acatccggcc
      901 tcagtaatta gatcaggaaa tcgtcgagag atttaccttc tgccagcgcc tgagcaattg
      961 gcttcggtgt acggccctga ccggtccagg ttttagtttc accgttaaca tcagtgaatt
     1021 tatatttcgc cggacgcggc tggcgttttt taccagcacg tggtgcagca gcagagctat
     1081 tacccaataa ctcttccggg ttaattccgt cagctttcat cagctccagc caggtgctaa
     1141 ttttttcctg acgctcagcc agttcacgct gctgctgttc ttcttcttca cgtctttctt
     1201 tagtgacaac cctgaatttt tcgagcattt cttcaagaac gtcaatggag aattcgcgag
     1261 ccatcgcacg gagggtgcga atgttattta aactttgtaa cattacggac ataataaaga
     1321 aaacctttta acgccaaaac aaaaaagtat ttcgtcgcgt agtgtaagcg atcacttatt
     1381 atatttccac aaaatttaat ctatttacag tcctgcgcga gattatttca gctgttattt
     1441 tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga gtaatttagg
     1501 ttattggtga acagagtggc gctctctgct cgctaactcc gcaagcctga caaaaccgaa
     1561 gtgaataaaa atgcaatata acagaataaa agaaaacacc cctcactggt gctttctgct
     1621 gttaatgtac attttctggc atcaaaaatg ctgtaaatga cccggtcatg acaaacaccg
     1681 aattctctac gcttatagcg ttacctcccc cccaaacttt cattagtgaa attaaaggca
     1741 agatcatatc taattgattt aattgaaaat aaatcatatt ggcatgtaaa aatccacaat
     1801 gtacaaaaaa cgagccgtta cggaatattt tatctacaaa aactaactaa ataaaaattt
     1861 ttcactaatt gattagtcat agacagcgat atacgctatg caaaaatgca gatggcaatg
     1921 agatccactg ctttcatctc cattaacatc ccattacgct tttattaagg agcattagca
     1981 tgttctcacc gcagtcacgc ttgcgtcatg cagttgcaga tacgttcgcg atggttgttt
     2041 actgttctgt cgtgaacatg tgtattgaag ttttcctctc cggaatgagc ttcgaacagt
     2101 ctttttattc cagattggta gcgattccgg tgaacatctt aattgcatgg ccatacggta
     2161 tgtaccgcga tctgtttatg cgcgcggcac gcaaagttag cccgtcgggc tggataaaaa
     2221 atctggctga tatcctggct tatgtgacgt tccagtcacc ggtgtatgtg gcgatcttgt
     2281 tagtggtggg cgcagactgg catcagatta tggcggcggt cagttcaaac atcgttgttt
     2341 cgatgttgat gggggcggtt tatggctact tcctcgatta ttgccgccga ctgtttaaag
     2401 tcagccgtta ccagcaggta aaagcctgac tcttcttttc gcgactggca tcgccagtcg
     2461 cgtctttaac tggcttcgcc aaataaacca ttcaaataac gttcaagcgc aacacgcgaa
     2521 ctaaagccat gcgggatacc gtaatgctcg tgtcgctcgc ctgctaaata gagcggaaat
     2581 tgctggtaat gatcgcaagt tttaatatcg gaagcgggtt cagccagtgt tgaactgcgt
     2641 tcttttaatg ttggatgaat taccagagcg gtacgtccca ttcgcgcttc gcgattaaca
     2701 tagacataat tttccccacg gcggtaacca tacgctttct gggttaccac atcgacagta
     2761 aaaccgactt tttcaagtac gcgcgccacc tcgtctggtc gtaaatacat atttgatcct
     2821 cgttattttc aaccgcgggc ctaccttacc tgattgcgca ttcacaacgc tttcagaaaa
     2881 gtccataaag ccgcgatgaa cctcagttaa gaaaattatg gtctacactg aaaattacat
     2941 cgaattctaa tgggggatca tatgtttaac cgaccgaacc gcaacgacgt tgatgatggc
     3001 gtgcaggata tccagaatga tgtcaatcaa ttagctgaca gcctcgaatc tgtattgaag
     3061 tcctggggca gcgacgccaa aggggaagct gaagccgcac gcagtaaagc tcaggcgttg
     3121 ctgaaagaaa ccagggcgcg aatgcatggt cgtactcgcg tccagcaagc cgcgcgcgat
     3181 gccgttggct gagcggattc ttttgttcgt gaaagaccct ggtgtagcgt gggtacagca
     3241 gctgcggtag gtatttttat cggcgcactg ttaagcatgc gcaaatcgta gtgcaaaaat
     3301 gataataaat acgcgtcttt gaccccgaag cctgtcttcg gggtttcttt ttgcctggtg
     3361 aatcacaaaa atccccctcc cccgtcacgc tcatatccag ggtaatttcg atcactattt
     3421 gctatatatt gtgtagttga atcttttttc aactacatct agtatctctg tatcaacaga
     3481 gagacaaccc gacgcgtatc atcgcgccgt atcttcattt taaacggaaa tacgaatcat
     3541 gcgcataact atttatactc gtaacgattg cgttcagtgc cacgccacca aacgggcgat
     3601 ggaaaaccgg ggctttgatt ttgaaatgat taatgtcgat cgcgttcctg aagcagcgga
     3661 agcgttgcgt gctcagggct ttcgtcagtt gccggtagtg attgctggcg atcttagctg
     3721 gtctggtttc cgtccggaca tgattaaccg tctgcatcca gcgccacacg cggccagtgc
     3781 atgagccagc tcgtctactt ctccagcagc tccgaaaaca cgcagcgttt tatcgaacgt
     3841 ttaggtctgc ccgcggtgcg catcccgctc aatgagcggg aacggattca ggtagacgag
     3901 ccttacatcc tgatcgtgcc ctcttacggc ggcggcggta cggctggcgc ggtgccacga
     3961 caggtaattc gctttttaaa cgacgagcac aaccgggcgt tgcttcgcgg cgttattgct
     4021 tctggtaatc gcaactttgg tgaggcgtat ggccgcgccg gagatgtgat tgcccggaaa
     4081 tgcggcgtgc cgtggctgta ccgttttgaa ctcatgggta cgcaaagcga tatcgaaaac
     4141 gttcgtaaag gagtaaccga attttggcaa cgacaaccgc agaatgcctg acgcaggaaa
     4201 cgatggatta ccacgcgctg aatgcgatgc ttaatcttta cgatagcgca ggtcgcattc
     4261 agttcgataa agaccgccag gccgttgacg cctttattgc gacgcatgtg cgtccgaaca
     4321 gtgtgacctt cagtagccag cagcagcgcc tgaactggct ggtcaacgaa ggttactatg
     4381 atgaaagcgt tcttaatcgc tactctcgcg actttgtcat tacgctgttt gcccacgcac
     4441 acaccagcgg ttttcgtttc cagacattcc tcggggcatg gaagttttac accagctata
     4501 cgttgaagac attcgacggt aaacgttatc tggaagattt tgccgatcga gtcacgatgg
     4561 tggcgctgac gctggcacaa ggcgatgaga cgctggcgtt gcaactgacc gatgaaatgc
     4621 tgtcaggacg ctttcagcca gccacgccaa cattcctcaa ctgcggtaag cagcagcgcg
     4681 gcgaactggt ttcctgtttt ttgctgcgta ttgaagacaa tatggagtcg attggtcggg
     4741 cggtaaattc cgcactgcag ctgtcgaaac gcggcggcgg cgtagcattt ttgctgtcga
     4801 atctgcggga agcgggcgcg ccaattaaac gtattgaaaa tcaatcttct ggcgtaattc
     4861 cggtgatgaa aatgttggaa gacgcatttt cctatgccaa ccaactcggc gcgcgtcagg
     4921 gggcaggtgc agtctattta catgctcatc atcccgatat tctgcgtttt ctcgacacga
     4981 aacgggaaaa tgccgacgaa aaaatccgca ttaaaacact gtcgctgggc gtggtgatcc
     5041 cggatatcac tttccatctg gcaaaagaga atgcgcagat ggcgctgttt tcgccctatg
     5101 acgttgagcg agtttatggc aagccgtttg ccgatatcgc cattagcgaa cactatgacg
     5161 aactggttgc cgatgaacgc atccgcaaaa aatacctcaa cgcccgtgat ttcttccagc
     5221 gactggcaga aatccagttt gagtccggct atccctacat catgtatgaa gacacagtaa
     5281 accgtgctaa ccctatcgcc gggcgcataa atatgagtaa tctctgctca gaaattttgc
     5341 aggttaacag cgcctcagag tatgacgaga atctcgacta tgcccgcaca ggccatgata
     5401 tttcctgcaa tttaggttcg ttgaatattg cgcacaccat ggattccccc gatttttccc
     5461 gcacggtaga gactgccgtg cgcggtttaa cggcggtgtc agatatgagt catatccgca
     5521 gcgtgccgtc catcgaagcc ggaaatgccg cctcgcacgc catcggactg gggcagatga
     5581 atttacatgg ttatctggcg agggaaggca tcgcttatgg ttcgccggaa gccctggatt
     5641 tcaccaatct ctatttctat accatcacct ggcacgcact gcgtacctcg atgttgctgg
     5701 cgcgcgaacg cggtgaaacc ttcgccgggt tcaaacagtc acgctatgcc agtggtgaat
     5761 atttcagcca atatctgcaa ggtaactggc agccgaaaac ggcgaaagtt ggcgaactgt
     5821 ttgcccgcag ctgtattacg ttacctaccc gtgagatgtg ggcgcaactg cgcgacgacg
     5881 tgatgcgcta cggcatatac aaccagaatc tccaggcggt gccgccaacc gggtctatct
     5941 cttatatcaa ccatgctacg tcgagtattc atccgattgt ggcgaaagta gagatacgca
     6001 aagagggcaa aacaggacgc gtttactacc ctgccccgtt tatgactaac gaaaatctgg
     6061 cgctgtatca ggacgcttac gaaattggcg cagaaaagat catcgacacc tacgcggaag
     6121 cgactcgcca tgtcgatcag gggctgtcgc tgacgctgtt tttccccgat accgccacca
     6181 ctcgcgatat caacaaagcg cagatttacg cctggcgcaa aggtatcaaa acgctctatt
     6241 acatccgcct gcgtcagatg gcgctggaag gcactgaaat tgaaggctgc gtctcctgtg
     6301 cactttaagg aatatctatg aaactctcac gtatcagcgc catcaactgg aacaagatat
     6361 ctgacgataa agatctggag gtgtggaatc gcctgaccag caatttctgg ctaccagaaa
     6421 aggtgccgct gtcaaacgat attcccgcct ggcagacatt aactgtcgta gaacaacaac
     6481 tgacgatgcg cgtttttact ggcctgacgc tgctcgacac gctgcaaaat gttatcggcg
     6541 cgccttcgct gatgcccgat gcgctcacgc ctcatgaaga ggcggtatta tcgaatatca
     6601 gctttatgga agcggttcat gcccgctctt acagttcgat tttctcgacg ctgtgccaga
     6661 ccaaagatgt cgatgccgcc tacgcctgga gtgaagaaaa cgcaccgttg cagcgaaaag
     6721 ctcagattat tcagcaacat tatcgcggtg atgatccgct gaaaaagaaa atcgccagtg
     6781 tgtttcttga atcttttttg ttctattccg gtttctggct gccgatgtat ttttccagcc
     6841 gcggaaagct gaccaatacc gcggacctga tccgtctgat tatccgcgat gaagcagtcc
     6901 acggttacta cataggctat aaatatcaga aaaacatgga aaagatatct ctgggacaac
     6961 gtgaagagtt gaagagtttc gccttcgatt tgttgctgga actctacgac aacgagttgc
     7021 aatacaccga tgagctgtac gccgaaaccc cgtgggctga cgatgtgaaa gcgtttctct
     7081 gttacaacac caataaggct ttgatgaatc tgggctacga accgttattt cccgcagaaa
     7141 tggcggaagt gaatccggca atcctcgccg cgctttcgcc gaatgccgat gaaaatcacg
     7201 atttcttttc cggttcaggc tcctcttatg tgatggggaa agcggttgaa acagaagatg
     7261 aagactggaa tttctgaggg tgttattttc aaaaatatca ctacccgcag cagggaaata
     7321 attcccgcca aatagctttt tatcacgcaa atattctgta gtgatctaca ctgatactct
     7381 gttgcattat tcgcctgaaa ctacaatatt caggcgtttt ttcgctatct atgacaaaaa
     7441 atatcaactt ttctcgattt gctctcagcc cttatatcac ggaaaattcc ggcgatttgc
     7501 tcgcatcaat attcatgcca catttgccct caggggttgc ctcagattct cagtatgtta
     7561 gggtagaaaa aagtgactat tcccattggg taatatatcg acataaacaa ataaagaaat
     7621 cattctattg catggccatt aaattaaaaa ttaaaaatct ttataaaata tttggtgagc
     7681 atccacagcg agcgttcaaa tatatcgaac aaggactttc aaaagaacaa attctggaaa
     7741 aaactgggct atcgcttggc gtaaaagacg ccagtctggc cattgaagaa ggcgagatat
     7801 ttgtcatcat gggattatcc ggctcgggta aatccacaat ggtacgcctt ctcaatcgcc
     7861 tgattgaacc cacccgcggg caagtgctga ttgatggtgt ggatattgcc aaaatatccg
     7921 acgccgaact ccgtgaggtg cgcagaaaaa agattgcgat ggtcttccag tcctttgcct
     7981 taatgccgca tatgaccgtg ctggacaata ctgcgttcgg tatggaattg gccggaatta
     8041 atgccgaaga acgccgggaa aaagcccttg atgcactgcg tcaggtcggg ctggaaaatt
     8101 atgcccacag ctacccggat gaactctctg gcgggatgcg tcaacgtgtg ggattagccc
     8161 gcgcgttagc gattaatccg gatatattat taatggacga agccttctcg gcgctcgatc
     8221 cattaattcg caccgagatg caggatgagc tggtaaaatt acaggcgaaa catcagcgca
     8281 ccattgtctt tatttcccac gatcttgatg aagccatgcg tattggcgac cgaattgcca
     8341 ttatgcaaaa tggtgaagtg gtacaggtcg gcacaccgga tgaaattctc aataatccgg
     8401 cgaatgatta tgtccgtacc ttcttccgtg gcgttgatat tagtcaggtg ttcagtgcga
     8461 aagatattgc ccgccggaca ccgaatggct taattcgtaa aactcctggc ttcggcccac
     8521 gttcggcact gaaattattg caggatgaag atcgcgaata tggctacgtt atcgaacgcg
     8581 gtaataagtt tgtcggcgca gtctccatcg attcgcttaa aaccgcgtta acgcagcagc
     8641 aaggtcttga tgcggcgctg attgatgcgc cgttagcagt cgatgcacaa acgcctctta
     8701 gcgagttgct ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc gacgaggacc
     8761 aacagtatgt cggcatcatt tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg
     8821 taaataatgg ctgatcaaaa taatccgtgg gataccacgc cagcggcgga cagtgccgcg
     8881 caatccgcag acgcctgggg tacaccgacg actgcaccgg ctgacggcgg tggtgctgac
     8941 tggctgacca gtacgcctgc gccaaacgtc gagcatttta atattctcga tccgttccat
     9001 aaaacgctga tcccgctcga cagttgggtc actgaaggga tcgactgggt tgttacccat
     9061 ttccgtcccg tcttccaggg cgtgcgcgtt ccggttgatt atatcctcaa cggtttccag
     9121 caattgctgc tgggtatgcc agcgccagtg gcgattatcg ttttcgctct catcacctgg
     9181 cagatttccg gggtcggaat gggcgtggcg acgctggtct cgctgattgc catcggcgca
     9241 atcggtgcct ggtcgcaggc aatggtgact ctggcgctgg tgttaactgc cctgctgttc
     9301 tgtatcgtca tcggtttgcc gttggggata tggctggcga gaagtccgcg agcggcgaaa
     9361 attattcgtc cactgcttga tgccatgcag accacgccag cgtttgttta tctggtgcca
     9421 atcgtcatgc tatttggtat cggtaacgtg ccgggcgtgg tggtgacgat catctttgcg
     9481 ctgccgccga ttatccgtct gaccattctg gggattaacc aggttccggc ggatctgatt
     9541 gaagcctcgc gctcattcgg tgccagcccg cgccagatgc tgttcaaagt tcagttaccg
     9601 ctggcgatgc cgaccattat ggcgggcgtt aaccagacgc tgatgctggc cctttctatg
     9661 gtggtcattg cctcgatgat tgccgtcggc gggctgggtc agatggtact tcgcggtatc
     9721 ggtcgtctgg atatggggct tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt
     9781 atcctcgatc gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc
     9841 tggtacacca ctggccctgt tggtctgctg acccgcccat tcattaagta actctgcact
     9901 tgcccggtga cgccgggcat tatcaccctg ccaaaaaaag gaataacaat gcgacatagc
     9961 gtactttttg cgacagcgtt tgccacgctt atctctacac aaacttttgc tgccgatctg
    10021 ccgggcaaag gcattactgt taatccagtt cagagcacca tcactgaaga aaccttccag
    10081 acgctgctgg tcagtcgtgc gctggagaaa ttaggttata ccgtcaacaa acccagcgaa
    10141 gtggattaca acgttggcta cacctcgctt gcttccggcg atgcaacctt caccgccgtg
    10201 aactggacgc cactccatga caacatgtac gaagctgccg gtggcgataa gaaattttat
    10261 cgtgaagggg tatttgttaa cggcgcagca cagggttacc tgatcgataa gaaaaccgcc
    10321 gaccaataca aaatcaccaa catcgcacaa ctgaaagatc cgaagatcgc caaactgttc
    10381 gataccaacg gcgacggtaa agcggattta accggttgta accctggctg gggctgcgaa
    10441 ggtgcgatca accaccagct tgccgcgtat gaactgacca acaccgtgac gcataatcag
    10501 gggaactacg cagcgatgat ggccgacacc atcagtcgct acaaagaggg caaaccggtg
    10561 ttttattaca cctggacgcc gtactgggtg agtaatgagc tgaagccagg gaaagatgtg
    10621 gtctggttgc aggtgccgtt ctccgcactg ccgggcgata aaaatgccga taccaaactg
    10681 ccgaatggcg cgaattacgg cttcccggtc agcaccatgc atatcgttgc caacaaagcc
    10741 tggaccgaga aaaatccggc ggcagccaaa ctgtttgcca ttatgcagtt gccagtggcc
    10801 gatattaacg cccagaacgc cattatgcat gacggcaaag cctcagaagg cgatattcag
    10861 ggccacgttg atggctggat caaagcccac cagcagcagt tcgatggctg ggtgaatgag
    10921 gcgctggcag cgcagaagta atttttattc gggcggataa ggcgttttcg cctcatccgc
    10981 cgctctgtac acaatgcctg atgcgacgct gacgcgtcct atcatgccta caaaccacat
    11041 cgcacatttc aacaagctat ctttcatccc atattcatca acatccgcta ttattgattt
    11101 ccagcttaat catcacctga tgaacaaaaa taatgactaa acctaatcat gagcttagcc
    11161 cggcgctgat cgtgctgatg tctatcgcca ccggtctggc ggtagccagt aactattacg
    11221 cccagccatt gctcgacacc atcgcgcgta acttttccct ttccgccagt tcggcaggct
    11281 ttattgttac cgccgcgcag ttgggctatg ccgcaggtct actgtttctt gttcccctcg
    11341 gtgatatgtt tgaacgccgc cgcctgattg tctcgatgac cttactggca gcgggcggta
    11401 tgttaatcac tgccagcagt cagtcactgg cgatgatgat cctcggtacg gcattaaccg
    11461 gtttattctc agtcgtggca caaattctgg ttccgctggc agcgacgctg gcttcaccgg
    11521 ataaacgcgg caaagtggtt ggcaccatta tgagcggtct gctgttgggg atattgctgg
    11581 cacgaaccgt tgccgggtta ctggcaaacc tcggcggctg gcgcaccgtc ttttgggttg
    11641 cttcggtgtt aatggcactg atggcgctgg cattatggcg tggtctgcca caaatgaaat
    11701 cagaaaccca cctcaactac ccacagttgt tgggttccgt tttcagcatg ttcatcagcg
    11761 ataagatcct gcgcacccgc gcgttgctgg gctgcatgac ctttgccaac ttcagcattc
    11821 tctggacctc aatggccttt ttgcttgccg ctccaccttt taactacagc gatggtgaca
    11881 ttggtctgtt tgggcttgcg ggagctgccg gagcgttggg cgctcgtccg gcgggcggtt
    11941 ttgccgacaa gggcaaatcg caccacaact ttcggtctgc ttctgctgtt actttcatgg
    12001 ctggcaatct ggtttggtca cacttccgta ctggcgttga ttatcggcat tctggtgctg
    12061 gacctcaccg tacagggcgt gcatatcact aaccagacgg taatttatcg aatacatcct
    12121 gatgcgcgta atcgcctgac cgcaggttac atgaccagct actttattgg cggtgccgcc
    12181 ggttcgctaa tttcagcctc agcctggcaa catggcggtt gggctggcgt ttgtctggct
    12241 ggcgcgacga ttgccctggt taacttactg gtctggtggc gaggttttca tcgtcaggaa
    12301 gccgcaaatt aagcaatcag caaccgtttt cgtggcttta cacttataag ggtgttaaga
    12361 agcccatcag tctgataagg ttaagatatt cattcagtct atttataata ttaacaatcg
    12421 ttaagcgtac actctatgga aagccctact ccacagcctg ctcctggttc ggcgaccttc
    12481 atggaaggat gcaaagacag tttaccgatt gttattagtt atattccggt ggcctttgcg
    12541 ttcggtctga atgcgacccg tctgggattc tctcctctcg aaagcgtttt tttctcctgc
    12601 atcatttatg caggcgcgag ccagttcgtc attaccgcga tgctggcagc cgggagtagt
    12661 ttgtgggttg ctgcactgag cgtcatggca atggatgttc gccatgtttt gtatggcccg
    12721 tcactgcgta gccgtattat tcagcgtctg caaaaatcga aaaccgccct atgggcgttt
    12781 ggcctgacgg atgaggtttt tgccgccgcc accgcaaaac tggtacgcaa taatcgccgc
    12841 tggagcgaga actggatgat cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt
    12901 acggtaatag gggcattctc cggcagcggc ttgctgcaag gttatcccgc cgttgaagct
    12961 gcattaggtt ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc tttccagcgc
    13021 aaacaatctc tttgcgttac cgcagcttta gttggtgccc ttgcaggcgt aacgctattt
    13081 tctattcccg tcgccattct ggcaggcatt gtctgtggct gcctcactgc gttaatccag
    13141 gcattctggc aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt tactggttgg
    13201 cgcggcgaat tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta atgcccgccc
    13261 aaccaaacgt ggcgcggtag gtattttgct cgacaccatt ggcatcgcct cgatatgcgc
    13321 tctgctggtt gtctctaccg caccagaagt gatgcacgat acacgccgtt tcgtgcccac
    13381 gctggtcggc ttcgcggtac tgggtgccag tttctataaa acacgcagca ttatcatccc
    13441 aacactgctt agtgcgctgg cctatgggct cgcctggaaa gtgatggcga ttatataacc
    13501 cacaagaatc atttttctaa aacaatacat ttactttatt tgtcactgtc gttactatat
    13561 cggctgaaat taatgaggtc atacccaaat ggatagttcg tttacgccca ttgaacaaat
    13621 gctaaaattt cgcgccagcc gccacgaaga ttttccttat caggagatcc ttctgactcg
    13681 tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc tgaaggctca
    13741 ggggattaac gagacgttgt ttatggcgtt gattacgctg gagtctcagg aaaaccacag
    13801 tattcagcct tctgaattaa gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat
    13861 tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt aaaagcgata acgatcgccg
    13921 ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg ttttaccacc
    13981 gcagcataac tgcctgcatc aactctggtc cgcgctcagc acaacagaaa aagatcagct
    14041 cgagcaaatc acccgcaaat tgctctcccg tctcgaccag atggaacaag acggtgtggt
    14101 tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa
    14161 atgaccggcc agcatcgcaa catgctggcc tttttggcaa gcaggtcggc tcagccgatg
    14221 agttaagaag atcgtggaga acaatatgag cgcaaatgcg gagactcaaa ccccgcagca
    14281 accggtaaag aagagcggca aacgtaagcg tctgctcctc cttctcacct tgctctttat
    14341 aattattgcc gtagcgatag ggatttattg gtttttggta ctgcgtcatt tcgaagaaac
    14401 cgatgacgca tacgtggcag ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt
    14461 gacgaaagtc tgggccgata acaccgattt tgtaaaagaa ggcgatgttc tggtcactct
    14521 cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa actgcactgg cttccagcgt
    14581 tcgccaaacc caccagctga tgattaacag caaacagttg caggcgaata ttgaggtgca
    14641 aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc
    14701 caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca gtgcccaggc
    14761 gcaactggac gtcgctattc aacaatacaa tgccaatcag gcgatgattc tggggactaa
    14821 actggaagat cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg cctagctggc
    14881 gctggagcgt acccgtattg tcagtccgat gaccggttat gtttcccgtc gtgccgtgca
    14941 accgggtgcg caaattagcc caacgacgcc gctgatggcg gtcgttccag ccaccaatat
    15001 gtgggtggat gccaacttta aagagacgca gattgccaat atgcgcatcg gtcagccggt
    15061 caccatcacc accgatattt acggcgatga tgtgaaatac accggtaaag tggttggtct
    15121 ggatatgggc acaggtagcg cgttctcact gcttccggcg caaaatgcga ccggtaactg
    15181 gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac agctggagca
    15241 atacccgctg cgcattggtt tgtccacgct ggtgagcgtc aataccacta accgtgacgg
    15301 tcaggtactg gcaaataaag tacgttcaac accggtcgcg gtaagtaccg cgcgtgaaat
    15361 cagcctggca cctgtcaata aactgatcga cgatatcgta aaagctaacg ctggctaatc
    15421 cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg gtcattatga
    15481 cgattgcgct gtcactggcg acattcatgc aggtgctgaa ctccaccatt gctaacgtgg
    15541 cgatccccac tatcgccggg aatctgggct catcgctcag ccaaggaacg tgggtaatca
    15601 cttctttcgg ggtggcgaat gccatctcga tcccgcttac cggctggctg gcaaagcgcg
    15661 tcggggaagt gaaactgttc ctttggtcca ccatcgcctt tgctattgcg tcttgggcgt
    15721 gtggtgtctc cagcagcctg aatatgctga tcttcttccg cgtgattcag gggattgtcg
    15781 ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa taactacccg ccagccaaac
    15841 gctcgatcgc gctggcgttg tggtcgatga cggtgattgt cgcgccaatt tgcggcccga
    15901 tcctcggcgg ttatatcagc gataattacc actggggctg gatattcttc atcaacgtgc
    15961 cgattggcgt ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgt gaaacccgca
    16021 ccgaacggcg gcggattgat gccgtggggc tggcactgct ggttattggt atcggcagcc
    16081 tgcagattat gctcgaccgc ggtaaagagc tggactgttt ttcatcacag gaaattatca
    16141 ttcttaccgt ggtggcggtg gtggctatct gcttcctgat tgtctgggag ctgaccgacg
    16201 ataacccgat agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc ggctgcttgt
    16261 gtatcagcct cgcgtatatg ctctacttcg gcgctattgt tctgctgccg cagttgttgc
    16321 aggaggtcta cggttacacg gcgacctggg caggtttggc ctctgcgccg gtagggatta
    16381 ttccggtgat tctgtcgccg attatcggcc gcttcgcgca taaactggat atgcggcggc
    16441 tggtaacctt cagctttatt atgtatgccg tctgcttcta ctggcgtgcc tatacctttg
    16501 aaccaggtat ggatttttgc gcgtcggcct ggccgcagtt tatccagggg tttgcggtgg
    16561 tctgcttctt tatgccgctg accaccatta cgctgtctgg tttgccaccg gaacgactgg
    16621 cggcggcatc gagcctctct aactttacgc gaacgctggc ggggtctatc ggcacgtcga
    16681 taaccacgac catgtggacc aaccgcgagt cgatgcacca tgcgcagttg actgagtcgg
    16741 taaacccgtt caacccgaat gcccaggcga tgtacagtca actggaaggg cttgggatga
    16801 cgcaacagca ggcatcaggc tggattgccc agcagatcac caatcagggg ctgattattt
    16861 ccgccaatga gatcttctgg atgtcagccg ggatattcct cgtactgctg gggctggtgt
    16921 ggtttgctaa accgccattt ggcgcaggtg gcggcggagg cggtgcgcac taagtacaac
    16981 taagccagtt catttgaact ggcttttttc aattaattgt gaagatagtt tactgactag
    17041 atgtgcagtt cctgcaactt ctctttcggc agtgccagtt cttcgttgct gttgatgcgt
    17101 acctcacgtt ccagaatgct acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg
    17161 taagtgccac actggtagac gttcagttcc gggatctgat tctgatcctg cactttcagc
    17221 acgtcttcca ttgccgcttt ccaggcatca gcaacacgct gctcatctgg cgtaccaatc
    17281 agactcatat aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca
    17341 ttaccgttaa gatggttacg cataaaacca gcaaacaggt gctccagggt atggatccct
    17401 ctttctggca tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg
    17461 tcgccatgcg gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta
    17521 tgatcgactg tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa
    17581 attttctgaa ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat
    17641 catcatccct gaattcagag atgaaatttt ggccactccc gagtggcctt tttcttttct
    17701 gtcaggcgtg tttttccagc cacaccgcaa acggttcggt atcagcggtt tccatttcct
    17761 gctgacggcg ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca
    17821 gcggctcttc acgcagcaga ttacggtagg cttctgcaaa cgctttgcct gttccgccaa
    17881 taccagtatc aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga
    17941 agcaggcaac cagttcatca cacactttct gatacgcttc gccgccgtta atgctatcca
    18001 gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt acccacctgc agtaacggga
    18061 actgcgcggt ttcgcagccg atacccagcg tcaggcccgg tttgcgacct tcaaggatca
    18121 cccggttcca gttaacgcgc gtacaggcaa gttcgctact gctcatttcc ggtgcatcag
    18181 ccagcgcaca ccagaccata aacaggtcga ggaatcgcac ctgctgttca tctacaccaa
    18241 tcggcgagaa cgggttgatg tccagcgaac gcacttcaat atattcaatg ccgccacgta
    18301 acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg ttttggacga atcggcgcat
    18361 acagttcgtt ttcaatctgc agcacgttgc tattgatttg cagcctctta ccgtctttct
    18421 caataccaat cttcgcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcta
    18481 cgtactcgta aagatcgttg aaggtaatac caagattgct ttgcgattta ttggtatagc
    18541 cgagatcgct caaacgaaga gaggtcgcat acggcaggta atacataccg cactcggttt
    18601 tctcaaacgg cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc gccggagatg
    18661 caccaaacag ataaggaata acccaaccga aacgatagta attgcggata acgcggaaat
    18721 agcccgcaga aattttctct ttggcatcag cgcccgagat atcaccgcac ttcgcttgcc
    18781 agaatgccat tggcaaagag aaattgtagt gcacgccgga aatggtttgc atcagcgcgc
    18841 cgtagcgatt tttcagccct tcacgataca gcgttttaaa gcgtccggtg ttagaagtgc
    18901 cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta gcatggcata cttaacggcc
    18961 acatccgctc atcgcccata ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca
    19021 gcatatgttc aatatcacca tccactggtg taatgaattc cagcaatgct tccgcaaaat
    19081 cggtagtaat ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg
    19141 ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct
    19201 ttaacgcctg aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat
    19261 tgacctcccg cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc
    19321 aggcctacaa ttagtgccac cacatcatgc cctgaacggt cgctgctgca actgcaacat
    19381 agcgtaacgc tttaccaagg cacaaaaaaa agattactgg cccccaggag atgcgcatcc
    19441 atcccgctaa cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga
    19501 ctgcaccata gcgtttcagc cagccggtag ctttctcttg ccagcgcgat gttttacgca
    19561 atggaaagaa acgcccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg
    19621 ctgttaaaac taaaacccag ggatgactga tcccggaaag caacattgcc accagcacga
    19681 cttcggagtt gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa
    19741 gcgataacgc ttcactcaca gcaagcgaac atccacagcg tccatgcctg ctgcacgggc
    19801 cgcctgaata ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat
    19861 acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga cgtgatcagc
    19921 agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg cctcagcgat
    19981 ggcgctttca ctccccgttc ctacagccat tgggcgacga ccatgccaac ttttcaccac
    20041 ttcaacaaga ggaagcggct cgacactatc cagcagcata cttcttactg cttctgtttt
    20101 ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc agctcaataa ttgcctgagc
    20161 aatacgccag gtgggcgatc cattaagcgc aatcatcgcc tgaatatcgt actgaagacc
    20221 gtagtgccct aatacttcgc gccacgcttt acggtgcgta ggcgccgtat ccaggattgt
    20281 gccatccata tcaaaaatta aacctgcata acgctcgtac atggtcttct cgcaagtcga
    20341 aaaatatgac gctactttag cgtaattgct tgattttgtc gctgatggcg aaggagaaga
    20401 aaattcagga aatggataaa gtagttattt agaaatgaga tatttttgaa ggaaattttt
    20461 tggagaatat ggtgcatccg ggaggattcg aacctccgac cgct
//
LOCUS       DAJTYH010000075        20303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTYH010000075 DAJTYH010000000
VERSION     DAJTYH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            214..555
                     /gene="raiA"
                     /locus_tag="OO609_002685"
     CDS             214..555
                     /gene="raiA"
                     /locus_tag="OO609_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6634049.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    658..783
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            659..706
                     /gene="pheL"
                     /locus_tag="OO609_002686"
     CDS             659..706
                     /gene="pheL"
                     /locus_tag="OO609_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6634050.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            805..1965
                     /gene="pheA"
                     /locus_tag="OO609_002687"
     CDS             805..1965
                     /gene="pheA"
                     /locus_tag="OO609_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6634051.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2008..3129)
                     /gene="tyrA"
                     /locus_tag="OO609_002688"
     CDS             complement(2008..3129)
                     /gene="tyrA"
                     /locus_tag="OO609_002688"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6634052.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3140..4210)
                     /gene="aroF"
                     /locus_tag="OO609_002689"
     CDS             complement(3140..4210)
                     /gene="aroF"
                     /locus_tag="OO609_002689"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6634053.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4420..4785
                     /locus_tag="OO609_002690"
     CDS             4420..4785
                     /locus_tag="OO609_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6634054.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4935..5453
                     /locus_tag="OO609_002691"
     CDS             4935..5453
                     /locus_tag="OO609_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6634055.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNVFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5443..6668
                     /gene="dgcN"
                     /locus_tag="OO609_002692"
                     /pseudo
     CDS             5443..6668
                     /gene="dgcN"
                     /locus_tag="OO609_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6684..7166
                     /locus_tag="OO609_002693"
     CDS             6684..7166
                     /locus_tag="OO609_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6634056.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7243..7590)
                     /gene="rplS"
                     /locus_tag="OO609_002694"
     CDS             complement(7243..7590)
                     /gene="rplS"
                     /locus_tag="OO609_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6634057.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7632..8399)
                     /gene="trmD"
                     /locus_tag="OO609_002695"
     CDS             complement(7632..8399)
                     /gene="trmD"
                     /locus_tag="OO609_002695"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6634058.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8430..8978)
                     /gene="rimM"
                     /locus_tag="OO609_002696"
     CDS             complement(8430..8978)
                     /gene="rimM"
                     /locus_tag="OO609_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6634059.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8997..9245)
                     /gene="rpsP"
                     /locus_tag="OO609_002697"
     CDS             complement(8997..9245)
                     /gene="rpsP"
                     /locus_tag="OO609_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6634060.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9382..10743)
                     /gene="ffh"
                     /locus_tag="OO609_002698"
     CDS             complement(9382..10743)
                     /gene="ffh"
                     /locus_tag="OO609_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6634061.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10910..11701
                     /locus_tag="OO609_002699"
     CDS             10910..11701
                     /locus_tag="OO609_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6634062.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11722..13008
                     /locus_tag="OO609_002700"
     CDS             11722..13008
                     /locus_tag="OO609_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6634063.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13063..13656)
                     /gene="grpE"
                     /locus_tag="OO609_002701"
     CDS             complement(13063..13656)
                     /gene="grpE"
                     /locus_tag="OO609_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6634064.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13779..14657
                     /gene="nadK"
                     /locus_tag="OO609_002702"
     CDS             13779..14657
                     /gene="nadK"
                     /locus_tag="OO609_002702"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6634065.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14743..16404
                     /gene="recN"
                     /locus_tag="OO609_002703"
     CDS             14743..16404
                     /gene="recN"
                     /locus_tag="OO609_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6634066.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16553..16894
                     /gene="bamE"
                     /locus_tag="OO609_002704"
     CDS             16553..16894
                     /gene="bamE"
                     /locus_tag="OO609_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6634067.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16956..17246)
                     /locus_tag="OO609_002705"
     CDS             complement(16956..17246)
                     /locus_tag="OO609_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6634068.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17236..17673)
                     /gene="ratA"
                     /locus_tag="OO609_002706"
     CDS             complement(17236..17673)
                     /gene="ratA"
                     /locus_tag="OO609_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6634069.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17844..18326
                     /gene="smpB"
                     /locus_tag="OO609_002707"
     CDS             17844..18326
                     /gene="smpB"
                     /locus_tag="OO609_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6634070.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18541..18903
                     /gene="ssrA"
                     /locus_tag="OO609_002708"
     tmRNA           18541..18903
                     /gene="ssrA"
                     /locus_tag="OO609_002708"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19108..>20303
                     /locus_tag="OO609_002709"
     CDS             19108..>20303
                     /locus_tag="OO609_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6634071.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRG"
BASE COUNT         5245 a         5317 c         4754 g         4987 t
ORIGIN      
        1 ttgtgaaact gaagcgattt agtcactatc gatctcatca aatatggctc gctttgagat
       61 attcctcaag taaaaaaaca cctcttcctg cgatttctca caaaaaagat tcgttgacaa
      121 aaagtgacaa aattatgaga tttccatcac acattttgac atcaggaacg gtatgctgaa
      181 ttcaccaaga cgggaagaca agaggtaaaa tttatgacaa tgaacattac cagcaaacaa
      241 atggaaatta ctccggccat ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa
      301 tggcaaacac atctgattaa tccacatatc attctgtcca aagagccaca agggtttgtt
      361 gctgacgcca caatcaatac acctaacggc gttctggttg ccagtggtaa acatgaagat
      421 atgtacaccg caattaacga attgatcaac aagctggaac ggcagctcaa taaactgcag
      481 cacaaaggcg aagcacgtcg tgccgcaaca tcggtgaaag acgccaactt cgtcgaagaa
      541 gttgaagaag agtagtcctt tatattgagt gtatcgccaa cgcgccttcg ggcgcgtttt
      601 ttgttgacag cgtgaaaaca gtacgggtac tgtactaaag tcacttaagg aaacaaacat
      661 gaaacacata ccgtttttct tcgcattctt ttttaccttc ccctgaatgg gaggcgtttc
      721 gtcgtgtgaa acagaatgcg aagacgaaca ataaggcctc ccaaatcggg gggccttttt
      781 tattgataac aaaaaggcaa cactatgaca tcggaaaacc cgttactggc gctgcgagag
      841 aaaatcagcg cgctggatga aaaattatta gcgttactgg cagaacggcg cgaactggcc
      901 gtcgaggtgg gaaaagccaa actgctctcg catcgcccgg tacgtgatat tgatcgtgaa
      961 cgcgatttgc tggaaagatt aattacgctc ggtaaagcgc accatctgga cgcccattac
     1021 attactcgcc tgttccagct catcattgaa gattccgtat taactcagca ggctttgctc
     1081 caacaacatc tcaataaaat taatccgcac tcagcacgca tcgcttttct cggccccaaa
     1141 ggttcttatt cccatcttgc ggcgcgccag tatgctgccc gtcactttga gcaattcatt
     1201 gaaagtggct gcgccaaatt tgccgatatt tttaatcagg tggaaaccgg ccaggccgac
     1261 tatgccgtcg taccgattga aaataccagc tccggtgcca taaacgacgt ttacgatctg
     1321 ctgcaacata ccagcttgtc gattgttggc gagatgacgt taactatcga ccattgtttg
     1381 ttggtctccg gcactactga tttatccacc atcaatacgg tctacagcca tccgcagcca
     1441 ttccagcaat gcagcaaatt ccttaatcgt tatccgcact ggaagattga atataccgaa
     1501 agtacgtctg cggcaatgga aaaggttgca caggcaaaat caccgcatgt tgctgcgttg
     1561 ggaagcgaag ctggcggcac tttgtacggt ttgcaggtac tggagcgtat tgaagcaaat
     1621 cagcgacaaa acttcacccg atttgtggtg ttggcgcgta aagccattaa cgtgtctgat
     1681 caggttccgg cgaaaaccac gttgttaatg gcgaccgggc aacaagccgg tgcgttggtt
     1741 gaagcgttgc tggtactgcg caaccacaat ctgattatga cccgtctgga atcacgcccg
     1801 attcacggta atccatggga agagatgttc tatctggata ttcaggctaa tcttgaatca
     1861 gcggaaatgc aaaaagcatt gaaagagtta ggggaaatca cccgttcaat gaaggtattg
     1921 ggctgttacc caagtgagaa cgtagtgcct gttgatccaa cctgatgaaa aggtgccgga
     1981 tgatgtgaat catccggcac tggattatta ctggcgattg tcattcgcct gacgcaataa
     2041 cacgcggctt tcactctgaa aacgctgtgc gtaatcgccg aaccagtgct ccaccttgcg
     2101 gaaactgtca ataaacgcct gcttatcgcc ctgctccagc aactcaatcg cctcgccgaa
     2161 acgcttatag taacgtttga ttaacgccag attacgctct gacgacataa tgatgtcggc
     2221 ataaagctgc ggatcctgag caaacagtcg cccgaccatc gccagctcaa ggcggtaaat
     2281 cggcgaagag agcgccagaa gttgctcaag ctgaacattt tcttctgcca ggtgcagccc
     2341 gtaagcaaaa gtagcaaagt ggcgcagagc ctgaataaac gccatattct gatcgtgctc
     2401 gacagcgcta atacgatgca accgagcgcc ccagacctga atttgctcca gaaaccattg
     2461 gtatgcttcc ggcttacgtc catcacacca gaccacaact tgctttgcca ggctaccgct
     2521 gtccgggccg aacatcgggt gtaaccccag taccgggcca tcgtgcgccg ccagcatggc
     2581 ctgtaatggt ccatttttca ctgatgccag atcaaccaga atacaatctt tcggtaaagg
     2641 cggtaatttg ccgataactt gctcagtaac gtggattggc acactaacaa tcaccattcc
     2701 ggcatcggca acaatatcag ccgctcgatc ccagtcatgt tgctccagaa tccgcacctg
     2761 ataacccgat agtgtcagca tcttctcgaa caggcgtccc atctgaccgc caccgccgac
     2821 gataaccacc ggacgcagtg acggacaaag tgttttaaat cctttgtcgt tttcactgga
     2881 gtaagattca cgcatcaccc gacgcaaaac atcctcaatc agatctggcg gtacacccag
     2941 agcttccgcc tctgcacgac gcgaggccaa catagatgcc tcgcgctccg gaacataaat
     3001 aggcagtcca aagcggcttt tcaccttgcc cacttcagca accagttcca gacgcttcgc
     3061 taataaattc agcagcgctt tatcgacttc atcaatttga tcgcgtaatg cggtcaattc
     3121 agcaaccata ataaacctct taagccacgc gagccgtcag ctgcccgttc agatcctgat
     3181 gaatttcacg cagcaaggca tcggtcattt cccagctaat gcaggcatcg gttacggata
     3241 caccgtattt catttcactg cgcggttgct cagaagactg attgccctcg tggatattac
     3301 tttcgatcat cagaccaata attgagcgat taccatcttt gatttgagca accacggatt
     3361 ctgccaccgc aggctgacgg cgataatctt tattggaatt accgtggctg caatctacca
     3421 tcagagacgg gcgcagtccc gcctgttcca tctctttttc acattgcgca acatccgcag
     3481 ggctatagtt cggcgcttta ccaccgcgca ggatcacatg cccgtccgga ttcccctgag
     3541 tttgtagcaa cgcaacctgc cctgcctggt taatgccaac aaaacagtgc ggctgggcgg
     3601 cggcgcgcat agcgttaatt gctgttgcca gactgccgtc ggtgccgttt ttaaaaccaa
     3661 ccggcatgga aagcccggag gccatttcac ggtgagtttg cgattccgtt gtacgagcac
     3721 caattgctga ccagctaaac agatcgccca ggtattgcgg gctattcgga tctaacgctt
     3781 ccgtcgccag tggcagtccc atattcacca gctcaagcaa caatttacgc gcgatttgca
     3841 gcccggcttc tacatcaaaa gagccatcca tatggggatc gttaattaac cctttccagc
     3901 cgacagtggt acggggtttt tcaaaataga cgcgcattac cagatagagg ctatcgctga
     3961 cctctgcggc aagagcttta aatcgacgag catattccag agcagtttcc ggatcatgaa
     4021 tggaacaagg accacatact accagcagac gaggatcgcg cccggcgata atatctgaaa
     4081 tggttttacg cgagtcagca atctgggctt cttgttgcag gctcaatgga aaagcggcct
     4141 tcagttgttc cggagtcatt aaaacctgtt cgtcggtaat atgtacgtta ttcagcgcgt
     4201 ctttatgcat gatggcgatc ctgtttatgc tcgtttgcga tagttgctcc tcagcgagga
     4261 tgacgtaacg ataacacata aagtaaagtt ttcaatccat atttcgtaca tttttattta
     4321 cacaggcaat ttagtcgcgc tttcaaccct tgcttctgta tagataaatt tacatcccct
     4381 ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc
     4441 cccttgttgg ctttactggt tagcggatgt cagattgatc cttatactca cgcgccaacc
     4501 ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg cagcgccata
     4561 aaagatgacg atgcatttag cgattcacag gcggatcgcg gtctatacct taaaggatat
     4621 gccgaagggc aaaagaaaac ttgccagacc gattttactt atgcccgggg actttccggt
     4681 aaaagctttc ctgccagctg caacaatgtt gaaaatgcca gccaactgca tgaagtatgg
     4741 caaaaaaggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa accccacaaa
     4801 taaattaaac ataagatttt actcattgtg ttttatttct cacattgatg acggtcgcat
     4861 gaaaaaatga tattttaatg agcatcctga aaaaatatcg atgcatttcg agcgaagatg
     4921 gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt ctcctgctga
     4981 cgggagcacc aatactcgct caagagcctt ctgacgttgc gaaaaacgtg cgtatgatgg
     5041 tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca aagttatgta
     5101 tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca tctttgcctt
     5161 atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc aatgtttttt
     5221 attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat ccgtcaaagg
     5281 cgttattatt aattgccgaa caaaataccg agtgcattat tggtagcgcc ttttgtctga
     5341 tcatccacaa taatgacgtc agatttgccg taaacctgga tgcctcatcg cgtagcggtg
     5401 taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga taacgataat
     5461 tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat gaccagcata
     5521 tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct gaccctgaaa
     5581 cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag tctggaagca
     5641 gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct gggccagcaa
     5701 gggcattttc aactgcagaa gtacgtgata agcagcaaaa tattctggca tcctggcatt
     5761 acccccgtaa ggatccaggc gatactttca gcaatttcat aagccactgg ctcttccctg
     5821 cccccatcat tcagccgatt cgtcacaatg gtgaaaccat tggcgaagta cgcttaaccg
     5881 ctcgcgacag ttcaatcagc cattttatct ggttttcgct cgccgtactg accggttgta
     5941 ttctgctggc atcaggcatc gcaattaccc tcacccgcca tttgcacaat ggcctggtgg
     6001 aagcactgaa aaatatcacc gatgtcgtac atgatgtgcg ttccaaccgc aatttttccc
     6061 gacgagtttc ggaagaacgt atcgctgagt ttcaccgctt cgctctcgac ttcaacagtc
     6121 tgctggatga aatggaagag tggcagcttc gtttacaggc caaaaatgcg cagcttctgc
     6181 gtaccgcgct acatgaccca ttaaccgggc tggctaaccg cgcagcgttt cgtagcggca
     6241 tcaatacgtt gatgaacaat tccgatgccc gaaaaacgtc ggcgttacta tttcttgatg
     6301 gcgataattt caaatatatc aatgatacct ggggtcatgc gacgggcgat agggtcttga
     6361 ttgaaatcgc aaaacggtta gctgaatttg gcgggctgcg acataaagca taccgcctgg
     6421 gcggcgatga attcgctatg gtgctctatg atgtacagtc ggaatctgaa gtgcagcaga
     6481 tatgctcagc actgacacaa atctttaatc tcccgtttga tcttcataat ggccatcaga
     6541 ccaccatgac attaagcatt ggttacgcga tgaccattga gcacgcctct gcggaaaaat
     6601 tacaagagct tgccgatcac aatatgtatc aggccaaaca ccagcgtgcc gaaaagctgg
     6661 tgagataaca aggatatatc gatatgataa agcacctggt agcacccctg attttcacct
     6721 cactaatact gactggctgc cagtcccctc agggaaagtt tactcctgag caagtcgccg
     6781 ctatgcaatc ttatggattt actgaatccg ccggcgactg gtcgctgggc ttatcagatg
     6841 ccattctgtt cgcaaaaaat gactacaaat tgctcccgga aagccagcaa cagatccaaa
     6901 ccatggcagc caaattggcc tcgacagggc taacacatgc ccgtatggat ggacacaccg
     6961 ataactatgg tgaagacagt tacaacgaag gcttatcatt gaaacgggcg aatgtcgtgg
     7021 ccgatgcatg ggctattggt ggacaaattc cacgcagcaa tctcaccaca cagggtttag
     7081 gaaaaaaata tcccatagcc agtaacaaga ccgcccaggg ccgcgccgag aaccgccgcg
     7141 tcgcagtggt gattgcaacc ccttaaaaca cgagcaaaca gacaaaaaaa gggccagcca
     7201 attggccagc ccttcttaac aggatgtcgc ttaagcgaaa tcttagttaa gacgctcttt
     7261 gatacgagca gccttaccag tacgctcacg caggtagtac agtttagctt tacgaacagc
     7321 accacgacgt ttgacagaaa tgctgtcaac taccggagag tgagtctgga agacacgctc
     7381 aacgccttcg ccgttggaaa ttttacgaac agtgaatgca gagtgcagac cgcggttacg
     7441 aatagcgata accacgccct cgaatgcctg cagacgtttt ttggaacctt caacaaccca
     7501 tactttcact tccacggtat cacccggacg gaaggaaggt acgtcctgct tcatctgctc
     7561 ttgttcaagt tgcttaataa tgttgctcat aatttaatct cttatcctgg gtaaactgat
     7621 ctctcggggg cttacgccat cccatcatgt ttatgttgct gttgtgcgtg ttccgttttg
     7681 aactccgcca gcaaccttgc ttgctcttca gtcagagcca ggttttccag aagttcaggt
     7741 cttctaagcc aggtacggcc cagcgactgt ttcaaacgcc agcgacgtat ctcggcatgg
     7801 ttgcccgaca gtaacactgg cggaacttcc atcccttcta acacctcagg ccgcgtatag
     7861 tgcgggcaat ccagcaatcc ttcagcaaag gaatcttccg ttgccgaggc ttcatgtccc
     7921 agtactcccg gaataaaccg ggaaacggag tcaatcagcg tcattgctgg taactcacca
     7981 ccactgagaa cgtaatcgcc gattgaccat tcttcgtcaa tttcggtttg gatcacgcgc
     8041 tcatctatac cttcgtagcg accgcacacc agaatcaatt tttgattcgt tgccagttcg
     8101 ctgacgcccg cttgatcaag cttgcgtccc tgtggtgaca gataaatcac ctttgcgcct
     8161 tcacccgccg cggcttttgc tgcatgaatg gcgtcccgca agggttgcac catcattaac
     8221 atccccggtc cgccgccgta aggacgatcg tccacggtac ggtgccggtc atgcgtgaag
     8281 tcgcgaggac tccaactctg gatgctcagc aggccatttt taactgcccg gccagttacc
     8341 ccgtaatcgg taattgcgcg gaacatttca ggaaacaggc taattatgcc aatccacata
     8401 gcgccgcctt ttaccgttta tccggtggtt taaaaaccag gatcccaatc tacttcgatt
     8461 gaacgagtag tgagatcgac tttcttgata acctgcccat cgaggaacgg tacgagacgt
     8521 tccttgatac caaacgcatc tttcaggttt gccttaatga cgagaacgtc attagatccg
     8581 gtttccatca tatcaacgac tttaccgaga tcgtagcctt cagtggttac tacctggcag
     8641 cccatcaggt ctttccagta gtagtccccc tcttcaagct gaggcagctg cgatgaatcc
     8701 acgacaattt cacaattcgt cagcaggttc gccgcatcac gatcgtcaac gcctttcagc
     8761 ttgatgatca tgtcctgatt gtggtgcttc cagctttcca gctggacttg ctgccactga
     8821 cccgccttct ggataaacca gggctgatag tcaaaaatgc tttcggcgtc ttcggtggaa
     8881 gaaaacactc tgagccaccc acgaataccg taagacgaac ccatttttcc caaaacgatg
     8941 ggatcaacag gtgcttgcgc ggtgagttgt ttgctcatca tgaccaccgt gacagattaa
     9001 gctgctttgt ttacttcttt gatcagcgca gcaacgcgat cagaaatagt tgcgccctgg
     9061 ccaacccagt gagcgatgcg atccagatcc aggcgagtgc cttcttcttt ttcgctagcg
     9121 attgggttga agaaaccaac gcgctcgatg aagcgaccgt tgcgtgcatt acggctgtca
     9181 gcgacaacaa cctggtagaa cggacgcttt ttagcgccgt gacgtgctaa acgaatagtt
     9241 accataacat cctcttgtgt gaataaaaca accggacccc atcgaggaac ggagtccggt
     9301 gtcatattaa aagcccgaaa attttactga tttctggaga aaatgcaatc agcagttaat
     9361 aactctgtcg cataaggtca tttagcgacc aggaaatccc ggcggcatca ttcccttcat
     9421 gcttctcatc atcttcgcca ttccgccttt cttcattttc ttcatcatgc gctgcatgtc
     9481 gtcgaactgc ttcagaaggc ggttaacgtc ctgtacctgc atcccgcaac cggcggcgat
     9541 acgacgctta cgcgaacctt tgatgatttc tggcttagcg cgctctttca tcgtcattga
     9601 gttgatgatg gcttccatac gcaccagcac tttatcgtcc atctgcgact tgacgttatc
     9661 cgggatctgc cccatgcccg gcagtttgcc catcaagctg gccatgccgc ccatattttt
     9721 catctggcgc agttgctcaa gaaagtcgtt gagatcgaag ccgtcgcctt tttttagctt
     9781 gctggctaat ttctctgcct gcgcgcggtc aactttgctt tcgatatctt cgatcagcga
     9841 cagtacgtcg cccatgccga gaatacgcga cgcgatgcgg tccggatgga acggctccag
     9901 cgcctcagtt ttctcgccaa caccgaggaa cttgatcggt ttgccagtga tgtgacgaat
     9961 agagagcgcc gcaccgccgc gggcatcgcc gtccactttg gtcaacacta cgccggtaag
    10021 cggtaacgct tcattgaatg cttttgccgt attggccgca tcctgaccgg tcatggcgtc
    10081 aaccacaaac agggtttcaa ccgggttaat cgacgcatgg acttgtttga tctcgtccat
    10141 catcgcttcg tcaacgtgca gacgaccagc ggtatccacc agcagcacgt cgtagaattt
    10201 cagtttggct tctttcagcg ccgcgttaac aatatcaacc ggcttctgac caacatcaga
    10261 agggaagaaa tcaacgccca cctgctctgc cagcgtctca agctgtttga ttgccgccgg
    10321 gcgatagacg tcagcagaaa ccaccagcac tttcttcttg tgcttctcgc gcaggaaatt
    10381 accgagttta ccaacgctgg ttgttttacc ggcaccttgc aggcctgcca tcagcacgac
    10441 cgcaggcggt tgcgcagcca gattcagggt ctggttctct tcgcccatcg ccgcaaccag
    10501 ttcgttacgg acgattttga cgaactcctg ccccggcgtc aggctcttat taacttcatg
    10561 accaaccgct ttctctttta cgcgattgat aaactcacgc actaccggca gagcgacgtc
    10621 cgcctccagc agcgccatgc gcacttcgcg cagcgtatct tttacgttgt cttcagtgag
    10681 gcgtccacgg ccactgatat tgcgcagcgt gcgcgacaaa cgatcggtta aattatcaaa
    10741 cattgtctct cgcctggggt ggaaacggtt ggccgcaatc gcgacacatc atcagtattt
    10801 tgccgcagta taacatgaag gcgtctttgt tgttatgcaa cggttggagc agcgttcacc
    10861 tgacgctata ctgcttctct ttcttattgc tcaaactgtc gacattacta tgcccgtttt
    10921 tgctctgctc gcgcttgtcg cctactccgt cagtcttgcg ctgattgttc ccggtctgct
    10981 gcaaaaaaac ggcggctggc ggcgcatggc tattatttct gcggtcattg cgctggtctg
    11041 ccacgcaatc gctctggaag cccgcatcct gcccgacggt gatagcggac aaaacctcag
    11101 cctgctgaac gttggttcat tggtcagttt ggtgatctgt acggtaatga ccattgtggc
    11161 ttctcgcaat cgtggctggt tgctgctacc cattgtctat gcctttgcgc ttatcaacct
    11221 ggcgctggcg accttcatgc ccaatgaata catcacccat ctggaagcaa cgcctgggat
    11281 gctgatgcac attggcttat cgctcttttc ctatgccacg ctaattatcg ccgccctgta
    11341 cgcgctgcaa ctggcgtgga ttgattacca actgaagaac aagaagctgg catttaacca
    11401 agaaatgccg ccattgatga gtatcgagcg taaaatgttc cacatcacgc agattggcgt
    11461 ggtgctgcta acgctcacgc tttgcactgg cctgttctac atgcacaacc tatttagcat
    11521 ggaaaatatc gacaaggctg tgctctctat cgtggcgtgg tttgtctata ttgtgctgct
    11581 gtggggacat taccatgaag gatggcgtgg acgccgcgtc gtctggttta acgttgcggg
    11641 cgcggtaatt ctgacactgg cctacttcgg cagccgaatt gtccagcagt taatcagcta
    11701 aacccagaaa aggagtttcc cctggaacac atttctacta ctacgttgat cattattctg
    11761 atcatcatgg tggtcatttc agcctatttt tccgggtccg aaaccggaat gatgaccctc
    11821 aaccgctatc gtcttcgaca tatggcgaaa cagggtaatc gctcggccaa acgcgtcgaa
    11881 aaattgctgc gtaagccaga ccgcctgata agcctggtgt taatcggcaa taacctggtc
    11941 aatattcttg cctccgcgct cggcactatt gtcgggatgc gtttgtacgg cgatgcgggc
    12001 gtggcaattg cgactggtgt gctgactttt gtcgtactgg tttttgctga ggtattgccg
    12061 aaaaccattg ccgcgctgta cccggaaaaa gtcgcttatc cgagtagttt tctgctggct
    12121 ccgctgcaaa ttttgatgat gccgctggtc tggttgctga atgctatcac ccgtatgctg
    12181 atgcgcatga tgggtatcaa aaccgatatc gtggttagcg gctctttgag caaagaagag
    12241 ttgcgcacta tcgtgcatga atcgcgctca caaatttccc gtcgcaatca ggatatgctg
    12301 ctgtcggtgc tcgatctgga aaaaatgacc gttgatgaca tcatggtacc gcgcagtgaa
    12361 attatcggta ttgatatcaa cgatgactgg aaatctattc tgcgccaact ctcccactca
    12421 cctcacgggc gcatcgtgct ttaccgtgat tcgctggacg acgccatcag tatgctgcga
    12481 gtacgtgaag cctggcggtt gatgtcggag aaaaaagagt tcaccaaaga aaccatgctg
    12541 cgcgccgcgg acgagatcta ttttgtgcct gaaggtacgc cgctcagcac gcagttggta
    12601 aagtttcagc gcaacaaaaa gaaagtcggc ctggtcgtca acgagtatgg agacattcag
    12661 gggctggtga cggttgaaga tattctggaa gagattgttg gcgatttcac tacgtcgatg
    12721 tcgccaacac ttgccgaaga ggtcacgccg caaaacgacg gttcggtgat tatcgatggc
    12781 accgccaacg tgcgggaaat caacaaagcc tttaactggc atctaccgga agatgatgcc
    12841 cgcacggtta atggcgtcat tcttgaggca ctggaagaga tccctgtcgc aggcacccgc
    12901 gtgcgtattg gcaagtacga tatcgatatt ctcgacgtac aggacaatat gattaagcag
    12961 gtaaaagttt ttcctgtgaa accgctgcgc gagagtgtgg cggagtaacg aaaacggccc
    13021 tgcattcgca tgcagggccg tgaattatta cgaaaacaga aattacgctt ttgctttcgc
    13081 tacagttacc atcgccgcac gaatcgtacg accattcagc gtataaccct tctgcataat
    13141 gcccagtacg ttacctggcg caacgtcatc agattccacc attgcgatag cctgatgcac
    13201 attcgggtcc agcggcacgt tagtttcggc gatcacttca acaccaaact tacgcacaac
    13261 atccagcatc gacttcagcg tcagctcaat gccttcaacc atcgcagaca tatccgggtt
    13321 agctttatca gccacttcca gcgcacgatc caggctatca atcaccggca gcaattcgtt
    13381 gatgaatttc tccagcgcga atttgtgggc tttttcaata tccagttcag tacgacgacg
    13441 caggttttcc atttcggctt ttacgcgcaa aataccgtca cgttcacggg tctgggcttc
    13501 agccagctga gcttcgagat tcgcaatttt ttcatcgcgc ggatccacct gctcagcaga
    13561 agcttctggc tcaactgcct caatctcttc gtgctgatcc atgataattt cttccggggc
    13621 ttgcccctca ggcgttttct gttctttact actcatgaat ttctccgcgt ttttttcgca
    13681 ttcatctcgc taacttcgct tattatgggg atcagtttca gggtttcaag ggaagcactc
    13741 acattgtcat caatcttcgc aacaaggacc tcggaaaaat gaataatcat ttcaagtgta
    13801 ttggcattgt gggacaccca cggcacccta ctgcactgac aacacatgaa atgctctacc
    13861 gctggctgtg cacaaaaggt tacgaggtca tcgttgagca acaaatcgct cacgaactgc
    13921 aactgaagaa tgtgaaaact ggcacgctcg cggagattgg gcaactggct gatctcgcgg
    13981 tagtcgttgg tggcgacggt aatatgctgg gcgcggcgcg cacgctcgcc cgttacgata
    14041 ttaaagttat tggaatcaac cgtggcaacc tgggtttcct gactgacctt gaccccgata
    14101 acgcccagca acaattagcc gatgtgctgg aaggtcacta catcagcgag aaacgttttt
    14161 tgctggaagc gcaagtctgt cagcaagatt gccaaaaacg catcagcacc gcgattaacg
    14221 aagtggtact tcatcctggc aaagtggcgc atatgataga gttcgaagtg tatatcgacg
    14281 agatctttgc gttttctcag cgatctgatg ggctaattat ttcgacgcca acaggctcca
    14341 ccgcctattc cctctctgcg ggcggtccta ttctgacccc ctctctggat gcgattaccc
    14401 tggtgcccat gttcccgcat acgttgtcag cacgaccact ggtcataaac agcagcagca
    14461 cgatccgtct gcgtttttcg catcgccgta acgacctgga aatcagttgc gacagccaga
    14521 tagcactgcc gattcaggaa ggtgaagatg tcctgattcg tcgctgtgat taccatctga
    14581 atctgattca tccgaaagat tacagttatt tcaatacatt aagcaccaag ctcggctggt
    14641 caaaaaaatt attctaattt tacgccagcc tctttactgt atataaaacc agtttatact
    14701 gtacacaata acagtaatgg tttttcatac aggaaaacga ctatgttggc acaactgacc
    14761 atcagcaact ttgctatcgt tcgtgagctt gagattgatt ttcatagcgg catgaccgta
    14821 ataactggcg agaccggtgc gggtaaatct attgcaatag atgccctcgg tctttgtctc
    14881 ggtggtcgcg ctgaagccga catggtgcgt accggcgctg ctcgcgctga cctgtgcgcc
    14941 cgtttttctc tgaaagatac gccagcggcc ctgcgctggc tggaagaaaa ccagcttgaa
    15001 gacgggcatg aatgtttgct tcgtcgcgtg atcagcagtg atggtcgctc ccgtggtttc
    15061 atcaacggta cagctgttcc tctgtcacaa ctgcgcgaac tgggtcagtt gctgattcag
    15121 atccatggtc agcacgctca tcaattactc accaaacctg agcaccaaaa attcctgctt
    15181 gatggctatg ccaatgaaac ctctctactt caggaaatga ccgcacgtta tcagttgtgg
    15241 catcaaagct gccgtgacct cgcgcatcat caacagttaa gtcaggaacg cgccgcccgt
    15301 gcggaactgc tgcaatacca attaaaagaa cttaacgaat ttaatccgca gcccggagag
    15361 tttgagcaaa tcgacgaaga gtacaaacgt ctggcgaaca gcggtcaatt gctgaccacc
    15421 agccagaatg cattggcatt aatggccgac ggtgaagacg caaacctgca aagtcagctt
    15481 tacacggcta aacaactggt gagcgaattg attggcatgg acagcaaact gtccggcgta
    15541 cttgatatgc tggaagaagc taccatccag attgctgaag ccagcgacga actgcgccac
    15601 tactgcgatc gtctggatct cgatcccaac cgactgtttg aacttgaaca gcgcatctca
    15661 aaacagattt cgctggcacg taaacatcac gtcagccctg aggcattacc acagtattac
    15721 cagtcgctac tggaagaaca gcagcaactg gacgatcagg ccgactcaca agaaacgctc
    15781 gcgctggcgg taacgaaaca tcatcagcag gcactggaaa ccgcgcgcgc attacaccaa
    15841 caacgccagc aatatgcaga agaacttgca cagctgatca ccgacagtat gcatgcgctc
    15901 tcaatgccgc atgggcagtt tacgattgat gtcaaatatg acgagcatca cctgggcgct
    15961 gacggtgccg atcgtattga gtttcgggta accaccaacc caggtcagcc aatgcagcct
    16021 attgccaaag tcgcatccgg tggtgaattg tcccgcatcg cactggcaat ccaggtcatc
    16081 acggcgcgta aaatggaaac cccggcactg atttttgatg aagtggatgt agggattagc
    16141 ggtccaacag cggcagttgt cggcaaactg ctgcgtcaac tcggcgaatc aactcaggtg
    16201 atgtgtgtta cccacctgcc acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc
    16261 aaagaaaccg atggtgcgat gacagaaaca catatgcaat ctctggataa aaaagcgcgg
    16321 ttacaagagc tggcgcgcct gcttggtggc agtgaagtca cacgtaacac actggcgaat
    16381 gcgaaagaac tgcttgcagc gtaaactttt ttcctgcttc acggtcagag taaacagcaa
    16441 aacgccgtaa gaccggaaag caaaaggttt taaagtgatg aaaggtctat tatcatcggc
    16501 atattacaga tgagccacgt actgctcggg cccgaaaagg aatcaaatca ctatgcgctg
    16561 taaaacgctg actgctgcag cagcagtact attgatgtta accgcaggct gttccactct
    16621 ggagcgagtg gtttaccgtc ctgacatcaa ccaggggaac tatctgaccg ctaacgacgt
    16681 atccaaaata cgtgttggca tgacgcaaca acaagttgcg tacgcattgg gtacaccgct
    16741 gatgtccgat ccatttggta cgaatacctg gttctatgtc ttccgccagc aaccaggtca
    16801 tgaaggtgta acgcagcaaa cgctgacgct gacctttaac agtagcggtg tgttgaccaa
    16861 tattgataac aaacctgcgc tgagtggtaa ctaataataa agttgctctc aaagacgtta
    16921 aaaaaggtgc tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct
    16981 cgttgcctgc gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg
    17041 ccatcatgca cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta
    17101 gttaaatcga tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc
    17161 gtcgcgccct cctgcagcgt cactcgctgc aggtattgct tctcaggtag cgcataagcc
    17221 acctcaacgg caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg
    17281 gaccatatta gccgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt
    17341 ggtaaactca aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa
    17401 cttccatcca ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg
    17461 gttactggtc aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc
    17521 taccgccgca gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacctgg
    17581 caaaaactga ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta
    17641 gggtaccagt gcggtccggc taatctgagg cataacaatt tccatcaaca aaaatccaac
    17701 aaataatatc atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg
    17761 ttcatgctaa gatagagcct tgtcccccgc aggattgata tggggtattt tcgatttcag
    17821 attaccgatg attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga
    17881 ccatcgcgct taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg
    17941 gacttgccct gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg
    18001 acagctacgt ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa
    18061 tggccgtggc ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca
    18121 accagcgcga actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg
    18181 cgctctccct gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta
    18241 aaaaacagca cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac
    18301 gcatcatgaa aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg
    18361 cgcctccctc tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg
    18421 ttgccatccc attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg
    18481 ataacgccat tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt
    18541 ggggctgatt ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt
    18601 ggcctcgtaa aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt
    18661 aataacctgc ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag
    18721 gtcaaaccca aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat
    18781 caggctagtt tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact
    18841 aagcatgtag taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca
    18901 ccaaaattct ccatcggtga ttaccagagt catccgatga agtcctaaga gcccgcacgg
    18961 cgcaagccct gcgggctttt ttgtgctccg aatttgtccc gcgaagtctg atgccaacta
    19021 attaaatccg atccttttag gcaccttgtt aggcacttca taaagctata ttgtctttga
    19081 ggtgcctaaa actatggaaa cccggcaatg gcaagacaaa ccaaacctct atccgttaag
    19141 gaaatcgaat ctgctaaacc caaggaagcg gactacgttc tctatgatgg cgatggcctt
    19201 gagctactca ttaaatccag cgggagtaaa atctggcagt ttcgctacat tcgccctgtc
    19261 accaagacac gagcaaagaa aagcataggc ccttacccgt cagttaccct tgccgatgcc
    19321 agaaactacc gtgcagagtc ccgctcactc ctagctaaac agattgatcc gcaggaacat
    19381 cagcaagaac aattgcgtag ttcgctggaa gccaaaacca atactttcca gctcgtagct
    19441 gaacgttggt ggaatgtgaa gaaagccagt gtgacagagg actatgcgga agatatctgg
    19501 cgctctcttg aaagagatgt ctttcctgcg attggcgacg ttagcgttac agatattaaa
    19561 gctcatacac tggttcaggc cgtacaaccg gttcaggcca gaggagcact ggaaacggtt
    19621 cgtcgcctgt gtcaacgtat taatgaggtc atgatctatg cccagaacac agggctgatt
    19681 gatgctgttc ccagcgttaa tatcggtaaa gccttcgaaa agcctcagaa aaagaacatg
    19741 cccagcattc gaccggatca gctatctcaa ctgatgcaga caatgcgaac agccagcatt
    19801 agcctttcca cacgctgcct gttcatgtgg caacttctta ctattacccg ccctgccgaa
    19861 gcggctgaag ctcgctggga agaagtagac atagaagcgc aagagtggaa gattcccgca
    19921 gcacgcatga aaatgaaccg cgaccatact gttccattgt cagatgaagc aatagctgtg
    19981 ctggagatga tgaaaccgct aagcggcaac cgagaattta tctttcctag ccgtatcaag
    20041 cccaaccagc ctatgaacag tcagactgtt aacgcctcac taaaacgcgc aggttttggt
    20101 ggtgtgcttg tttcgcatgg tctgagatca attgccagta cggctcttaa tgaacaaggc
    20161 tttccgcctg atgccattga ggcagcattg gcccatgtgg ataagaatga ggttcgtcgt
    20221 gcttataacc gcagcgatta cctggaacag cgtcgcccga tgatgcaatg gtgggcaaat
    20281 tttgtgatgg cagcggatcg tgg
//
LOCUS       DAJTYH010000076        19845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTYH010000076 DAJTYH010000000
VERSION     DAJTYH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(28..786)
                     /gene="aroD"
                     /locus_tag="OO609_002710"
     CDS             complement(28..786)
                     /gene="aroD"
                     /locus_tag="OO609_002710"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6634072.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(817..1683)
                     /gene="ydiB"
                     /locus_tag="OO609_002711"
     CDS             complement(817..1683)
                     /gene="ydiB"
                     /locus_tag="OO609_002711"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6634073.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1695..2967)
                     /locus_tag="OO609_002712"
                     /pseudo
     CDS             complement(1695..2967)
                     /locus_tag="OO609_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3150..4355)
                     /locus_tag="OO609_002713"
     CDS             complement(3150..4355)
                     /locus_tag="OO609_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6634074.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4455..4811)
                     /locus_tag="OO609_002714"
     CDS             complement(4455..4811)
                     /locus_tag="OO609_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6634075.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4947..5054)
                     /gene="rprA"
                     /locus_tag="OO609_002715"
     ncRNA           complement(4947..5054)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO609_002715"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5240..6352)
                     /gene="ydiK"
                     /locus_tag="OO609_002716"
     CDS             complement(5240..6352)
                     /gene="ydiK"
                     /locus_tag="OO609_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6634076.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6741..9797
                     /locus_tag="OO609_002717"
     CDS             6741..9797
                     /locus_tag="OO609_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6634077.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9794..10204
                     /gene="menI"
                     /locus_tag="OO609_002718"
     CDS             9794..10204
                     /gene="menI"
                     /locus_tag="OO609_002718"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6634078.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10304..10492
                     /locus_tag="OO609_002719"
     CDS             10304..10492
                     /locus_tag="OO609_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6634079.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11040..11408
                     /gene="sufA"
                     /locus_tag="OO609_002720"
     CDS             11040..11408
                     /gene="sufA"
                     /locus_tag="OO609_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6634080.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11417..12904
                     /gene="sufB"
                     /locus_tag="OO609_002721"
     CDS             11417..12904
                     /gene="sufB"
                     /locus_tag="OO609_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6634081.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12914..13660
                     /gene="sufC"
                     /locus_tag="OO609_002722"
     CDS             12914..13660
                     /gene="sufC"
                     /locus_tag="OO609_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6634082.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13635..14906
                     /gene="sufD"
                     /locus_tag="OO609_002723"
     CDS             13635..14906
                     /gene="sufD"
                     /locus_tag="OO609_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6634083.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14903..16123
                     /gene="sufS"
                     /locus_tag="OO609_002724"
     CDS             14903..16123
                     /gene="sufS"
                     /locus_tag="OO609_002724"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6634084.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16136..16552
                     /gene="sufE"
                     /locus_tag="OO609_002725"
     CDS             16136..16552
                     /gene="sufE"
                     /locus_tag="OO609_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6634085.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16701..17705
                     /gene="ldtE"
                     /locus_tag="OO609_002726"
     CDS             16701..17705
                     /gene="ldtE"
                     /locus_tag="OO609_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6634086.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17768..18004)
                     /gene="lpp"
                     /locus_tag="OO609_002727"
     CDS             complement(17768..18004)
                     /gene="lpp"
                     /locus_tag="OO609_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6634087.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18315..19727)
                     /gene="pykF"
                     /locus_tag="OO609_002728"
     CDS             complement(18315..19727)
                     /gene="pykF"
                     /locus_tag="OO609_002728"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6634088.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5181 a         4794 c         5080 g         4790 t
ORIGIN      
        1 agggtatttc cgccgaaata ttattgctta tgcctgatgt aaaatagtta ataccgtgcg
       61 caaatcattt accgagattt gccctggcgc agaggctttt tttaccgcac caaaagttgc
      121 cgccgagcca aatacttcac caaccagacg agaaattacg ccagtttttg ccatcgacat
      181 cgtgatgatt ggacgatcgg catactgctc ctgcatctcc agggtcgcgg caagcaacgt
      241 cagcacatcg ctggtacttt gcggcatcag cgcaatctta ggaatatcgg cgtcgaagga
      301 ctgcattttg cgcagacggg caatgatttc ttcggcttcc ggcgttttat ggaagtcatg
      361 gttggacatg acaactttca catcatgcgc gtgggcgtag gcgacggttt ctttgacctg
      421 atcatcgccg gtaaataact ccagatcgat catatcaacc aggccgctgt cgatggctgc
      481 acgattgaga gcaatataag cctcggtgga aatcgcctgc tcgccgcctt ctttggcact
      541 gcggaaggta aacagcagcg gtttttctgg catggtttca cggagaattt ttgccgccgc
      601 catgacagac tccacattgg agaggtcggc aaagtggtcc acacgccatt ccagaatatc
      661 aaagtccgct tcacgatagg cgagagcttc ggatttcacg cgggcgatat ctttcgccat
      721 cagcgagacg atgattttag gtgcgcccgc accaatgacg agatctttta cagttacggt
      781 tttcattttt taccctttct gcacgcggtc agcctgtcag gcaccgaacc ccatgacctg
      841 tttaacatat tccagaggga aatctttgcc agtccataac gtgaactgtt cagccccttg
      901 ccacaacaac atgccgtatc catcaatcgt tttgcaacca gcttgttgtg cctgctgcaa
      961 taacttcgtc atatgcgggt tatacacgca ttcagtgacc agcagtcccg gatgtaacag
     1021 actgatatca ttaaccaatg attcattctc aaggggtttc atacctactt ttgtgccatt
     1081 ggttaaaatg tcggcggaag ccagggcttc agcaaaggct tgctgatcgg cgagatcggt
     1141 atccgtgacg acacaatcgg tgttttcgtt aacccgctgc gcgaaggcga gggctttatc
     1201 gaagaactca tcccgacggt taaagagttt aatttctttt aaaccttcaa ttgccccctg
     1261 cgcgccaatc gccgttgagg cacctccggc ccctaacagc accatcgttt tgcctttgat
     1321 atcaaaaccg ctctctttaa tggcgcgaat atggcccgtg ccgtcggtgt tatagccacg
     1381 cagatagcca tcatcattaa cgatggtgtt gatggcaccc accagtttgg ctgctggcgt
     1441 taattcatca acatattcac acgccagttg tttgttcggc atcgacacac cagttccgcg
     1501 cattttgagg gcttttaatc cttcaattgc tccgggaaag ctatcgttat ccacttcgaa
     1561 ggccatatag gtaaatggca atcccgcttt ttctaaggct ttattctgca tttcaggcga
     1621 taaactgtgg cggataggat aagccatcaa tccaatcaat tcgtattttg cggtaacatc
     1681 cataattaac tcctttaacc tctatgctta attgtgttta accgggtgct aaatttgcgt
     1741 tcgccaaacc gcacatcatt ctcaggaata ataaaaacgc ggtaatagcg gctaaaaaca
     1801 ataattgcgg taatcagggc cagcagcgcg aaagtaaaat cgagaacaat gatatattgc
     1861 aggccgatgt tcgacagata accggtaatc agcggaataa caaagttagc cagtccaccc
     1921 atcatcatat aaatactggt gactttggct ttgcttttgg ggaaaaactc tgacatgacc
     1981 gaaacaccga gctgtaaaat gccgccagct gctgagaaac cgataacaaa ggcgccggca
     2041 ttacacacca gtggagaagg gtacaggtag ataatggcag ctgttattgt tgccagcgca
     2101 gagttaaata cattagccca gatgggccgg accatttttt tcagtagtgc ggcaaaaata
     2161 aagacacaga ccaacgagcc catactgtaa taagagatgg tttttaatgc ctcagcttct
     2221 gacataccag caaaagccat cgcatatttg ggcatccaca ccacaatcac ataaaaggtc
     2281 gagaatgcgg ctacaccgaa cagtaccgat gaaacacctt ccagccagac taaaggtttt
     2341 gctgttcatt tgcggtaatt cattagctac gctggcgtcc accaactggc tggggaattt
     2401 gcttttcaac agcatcagcg tgatcagtac aaacagaata cccggaataa tcagcccata
     2461 gccgtaccag atattattga gcaacatata gctcaccagc attgggtaga acatttgccc
     2521 aaatgacacc atcgctttaa ccagtatgac cgccgaacca gaggctttcg gaaagcattc
     2581 catgagcgcg gggtagccac ccgtatccag cgctgagtta gcgataccta cgcacactgc
     2641 cagaccgtag gcgagagtta aattcgggca agcgggaata ccaaagaaga atagcagata
     2701 cattattact gccattaata tcaccgcccg acgaccaaac ttatcggaga tcacaccgaa
     2761 gaataaaata ctgatcaatc gccccaaacc gataccggaa attaaatagg caatgcccgc
     2821 gttgtcagtg gaaaactttt ctgacagaga tgtcatattt tgggcaagcg taataacact
     2881 aatgccgtgc aggaaatagc tgaagtaaat acaaagaaca gccaggataa atggcgtgct
     2941 gaaagataat ttcgttaccg tacgtattgt tctccgacgg tttacataat tactatactg
     3001 aagatgattt tccctgggta catggcataa agtttatgtt tccaggaata ttgcatgtca
     3061 tagtttgttt ttgatcgcga ttttccttag ttagcgcaga aagagttatt tatccgtaag
     3121 atcttgcatg acctgaaaag aatattgcat tacccacccg gagcgacatt ttcctttagc
     3181 gagtgatgcc gcgttttttt gcggctgcgt aagccgataa acagtgccag taaaaaaccg
     3241 atggcagtat cgaaccacat aatatcggca atacttcttt gggacagatg agccgtaatc
     3301 aacggaatag taaaggtcgc aatactgccc gtactgtaat agatccctgt agctttacct
     3361 ttagcgtaag ggaaacgttc agccattaac gtcaggccaa tttgcacaac acctccagca
     3421 gaggtaaaac caatgacaaa agcaaatatt atcaccacat aaaatgtggg atgcaggcag
     3481 acggtaaaca gagcaataaa tgagataaag gtgtacagca tcagtaatgt tgttgggcga
     3541 acggtattac gaatgagtgg agcggtaata aatacacaaa gcagcgaacc cacggtgtag
     3601 atactgagta gtttgatcga catagtgtat gacatgcctg caacaaattg tccgtactgt
     3661 gccagccact ggctaaccag ataaaacgtt gccattgaga tatagccata taaggtataa
     3721 ctggctaaat cgataattga acagcgatgt tccgtagagc tggtggtttt ctttatgaca
     3781 ggtaagcgac gacccggatg gggtgggaac gtacaacggt ataaaaacag agcgttaata
     3841 aacataatgc ctgcagcaat cataaaggac caaccgaacc acagttcagc ccacactaac
     3901 aggctaatga ttagcggtaa taaaaattgt ccgctggaaa caaatgcttt aattaaaata
     3961 ttggctgtcc caggtgagcg tggaaaagct tccatcaaac tgggataagt gcctgcatcg
     4021 agaaaactgt ttgccattcc cgccagaaag ccaaaaacat aagcgataat gatgttattg
     4081 gtctgcagga tgtcaaaaaa gaaggccata tagcagcaca tcccgagcat gataaaaggg
     4141 cggcgaccaa agcgatcgga taataatcct gcaaaaagca agacacttaa tcgaccaatg
     4201 cccagcgatg agataactat cgagacaccc gcggcattag tctgccaaag tgtctccagc
     4261 gaggccatat tcaggctcat caaaatgacg cccataccat gcaccaggta attaaaatac
     4321 aacccaagtg cggtagggta atagggattt ttcataggtt ctacttagca aaggtgcttt
     4381 aaaaagtgta gcgatgttgt gtcagtatcc attcattatt gttgtaacaa cgacaattcc
     4441 tttctgtgga aaaattaaca aagccgctcc agatcgtgtg catataattc agcggctacc
     4501 gattgataga tcttccagtc aataaaattg ccatcagggt agacttgttg aaacgcggtt
     4561 aagtcgggga aaaaacgcat ggtgttgttg ccgatgcggt tatttatttt ctctatgatg
     4621 gcatgaagat gtttttttcg ttgctgacgc atagccaaca gttgctcgac tacacaatca
     4681 ggaatggcgc atttgccatt ttcccactga cgccagcttt cactgtcacc ggtctgggca
     4741 atccaggtcg cgcattcatc aatagtcatg gcaaaaatat ggcggagtgc ctggagttca
     4801 taagcgttca tcgtgtaatg gctgcatgaa ataaacaata aaatataaat aacattatca
     4861 gcctgctgac ggcttgaaga gagtcacagt atcttgtgca acattattgg aaagagtgag
     4921 gggcgaggta gcgaagcgga aaaatgttaa aaaaaagccc atcgtgggag atgggaaaag
     4981 actacacaca gcaattcgtt gtttcactca ggggatttcc atgcttataa atcaatatgt
     5041 tgatttataa ccgtgagcta atagtaggca tacggacttt ttgcgtcgat cagattcgtc
     5101 tcattagttt aaaaactgta aagaatttgc gacaggtact accagctgaa tggcggtaag
     5161 caggaaaata tggggaagag caagcaaata tcaattagcg gtgattaaca atcaggcggc
     5221 agagccgcct gatgagaaat tacttattcg gtttctcgat ttcgccaagt tcttcgagaa
     5281 tttcttccgg ttggtccgtc ggtggcggga cttcttccac ccacgcggca aacagacgcc
     5341 aggaaacggc taacagaacc ggaccaataa acagaccgat catcccgaaa gcaatcaaac
     5401 caccaataac gccagagagg atcaggatca gcggtaaatc ggcacccatg cgaattaaca
     5461 ttgggcggat gacgttatcc agcgtgccaa ccacaccgct ccacactaac aataccgttc
     5521 cccaggtggt atcgccggtc cagtagagcc agataatcgc cggaatcagt accggcaacg
     5581 ggccaagctg gacaaggcag gagaggatca ttaacaccgt tagcaacgtt gcataaggta
     5641 cgccggatac ggcgaggccg ataccgccaa gcactgcctg tactaacgcc gtcaccacca
     5701 cacccagcgc caccgcgcgg atagcctgtg ccgccagcag cacggcggca tctccgcgaa
     5761 cgcctgccag acgggttgca aaatggcgaa tgccttgtgc cacctgttca ccgcgccagt
     5821 acagcagggc actgaagaga agcatcagcg cacaatgcac cataaagcgc ccgatatgcg
     5881 ccgcctgccc aacgaaccag gtggtggtgg tgccaatata agggcggact ttcgccatga
     5941 tcgccgtacc ccccatatcc agcaagttgt gccagcctgc atacagcttc gcgccaatca
     6001 ccggaatggt attaagccac gccagatcgg gtaacgtcat gtcgccgctg gaaatggctt
     6061 taattagcgg gccgctgccg tcgacgatac tgttaaccag caaagcaata gggatgataa
     6121 acaccatcac taataacagc gtcatcacca gaacggcgag agagcggcgg ccaaacatga
     6181 tcttttgcaa acgtaacaat accggccagg tggcgataac caccgtaccg gcccatgcaa
     6241 agccgagaat aaagggttga acaatccaca gacatgccac aatcatgatg gctaaaaaca
     6301 gcaccgaaag cagaatttgt gcgacatccc tgggctgacg aacatttacc ataactactt
     6361 ttcacctttt ttgtgcgtca aatcgtcgac gcgacgtgaa cacgcgaaac tcacctgcat
     6421 aatgatgagc aatttcagcg gtttttaaca ggccgattct gcccttaatt ctgatgggcg
     6481 tgttagaaaa aaatgtggta caacaactca ggcaacacgc aaacgattta ctcgtcgtat
     6541 ttcaagttgc atgtgctgcg gctacgttcg ttcaccccag tcacttacta ttgtaagctc
     6601 ctggagttca ctcgcttgcc gccttcctgc aactcgaaat ccatagagta aataaacatc
     6661 acctttcagt gcgctattgc agccgcctga aagaatagtg tatcgaccta catcacagac
     6721 cgcaggaaag ggtcaatata atgattccac agatttccct ggcacccggc gtcgttcaac
     6781 tggtgcttaa ttttttgcaa gagctggagc aacaaggttt taccggcgat gcggcgacaa
     6841 gttatgccga tcgtctgaca atgtcgaccg acaacagtat ttaccaactt ctccccgatg
     6901 cggtggtatt tccgcgttca accgcagatg tggcgctgat cgcccgtctt gccgcgcagg
     6961 aacgctattc atcgctgatc tttacccccc gcggcggcgg caccggcact aacggtcagg
     7021 cgctcaacca ggggattatt gttgatatgt cccgccatat gaaccgcatc atcgaaatta
     7081 accctgaaga gggctgggtg cgcgttgagg ccggggtgat aaaagaccaa ctcaatcagt
     7141 acctgaaacc gttcggctac ttttttgcgc cggaactttc gaccagcaac cgggcaacgc
     7201 tcggtgggat gatcaatacc gatgcatccg gtcagggatc gctggtctat ggcaaaacgt
     7261 cagatcacgt acttggcgta cgcgcggtgt tgttgggggg cgatattctc gatacgcaac
     7321 ctttacccgt cgagctggcg gaaacgctgg gtaaatccaa taccacaatc gggcgaattt
     7381 ataacacggt ttatcaacgt tgccgtcagc aacgccagtt aatcatcgac aacttcccca
     7441 aacttaaccg ctttcttacc ggttacgatc tgcgtcatgt ctttaacgat gagatgaccg
     7501 agttcgacct gacgcgcatt ctgacgggtt cagaagggac gctggccttt attaccgaag
     7561 cgcggctgga tattacgcgc ttgcctaaag tgcgccgtct ggtgaatgtc aaatatgact
     7621 cttttgactc tgcgctgcgt aacgcgccgt ttatggttga ggcgcgggcg ctttcggtag
     7681 agacggtgga ctcaaaagtg ctgaatctgg cgcgggaaga tattgtctgg cattccgtca
     7741 gcgagttgat taccgatatg cctgacaaag agatgctcgg actgaacatt gtggaatttg
     7801 ctggtgatga tgaggcgctg attgacgagc gagttaatgc actctgtgtg cggcttgatg
     7861 agctgattgc cagccaacag gcaggtgtga ttggctggca ggtgtgccgc gagctggcgg
     7921 gggttgaacg tatctatgcg atgcgcaaaa aagccgttgg tctgcttggc aatgccaaag
     7981 gtgccgctaa gccaattccg tttgctgagg atacctgcgt accgccggaa catctggccg
     8041 attatattgc tgaatttcgc gcgctgctcg acagccacgg cttaagctac ggtatgttcg
     8101 gtcacgtcga cgcaggtgtc ttgcacgtcc gtccggcact ggatatgtgc gatccgcaac
     8161 aagagatttt gatgaagcaa atctctgatg acgtggtggc gctgactgcg aaatacggtg
     8221 gtttgttgtg gggcgagcac ggcaaaggtt ttcgcgctga atacagcccg gcgtttttcg
     8281 gtgaggaact ttttgcagaa ctgcgcaaag tgaaagcggc atttgacccg cataaccgac
     8341 tcaacccagg gaagatttgc ccgccagaag gtctcgatgc gccgatgatg aaagtggacg
     8401 cggtgaagcg cggtacattc gatcggcaga tccccattgc ggtacgccag cagtggcgcg
     8461 gtgcgatgga gtgtaacggc aacggtttat gcttcaactt tgatgcccgt agtccgatgt
     8521 gtccgtcgat gaagatcacc cagaaccgga ttcattcacc gaaagggcgc gcaacgctgg
     8581 tgcgtgaatg gctgcgcttg ttggcggatc gcggcgttga cccactcaaa ctggaacaag
     8641 aactgcctga atccggcgtc agtttgcgga cgttaattgc ccgcacgcgc aatagctggc
     8701 atgcgaataa aggcgaatat gacttctcac acgaagtcaa agaggcgatg tcgggctgtc
     8761 tggcctgtaa agcttgttcg acccagtgcc ccatcaaaat tgatgtgccg gagtttcgct
     8821 ctcgttttct gcagctctat cacacccgtt atttacgccc gcttcgcgac cacctcgttg
     8881 ctacggtcga gagctacgca ccgctgatgg cacgcgcgcc gaaaaccttt aacttcttca
     8941 ttaaccagcc gctggtgcgc aaactctcgg aaaaacatat cggcatggtt gatttgccac
     9001 tgctgtcggt cccctcgcta caacaacaaa tggtggggca tcgctcggca aacatgacgc
     9061 tggaacagct tgaagctctc aatgcagagc agaaagcgcg cacggtgttg gtggtgcagg
     9121 acccctttac cagctattac gatgcgcaag tggtggcgga ttttgtccgt ctggttgaaa
     9181 aattaggttt ccagcctgtg ttactgccat tttcgccaaa tggcaaagcc cagcatatca
     9241 aaggctttct taatcgtttt gcgaagacgg cgaaaaagac ggcggatttc ctcaaccgta
     9301 tggctaagct tgggatgcca atggtgggcg tcgatccggc gctggtactt tgttatcgcg
     9361 atgaatataa actggcgctg ggcgaggaac gtggcgagtt taacgtctta cttgcgaatg
     9421 aatggctggc aagcgcactt gagtcacatc cagtggctac agtcagcggt gaatcatggt
     9481 atttctttgg tcactgtacc gaggttaccg ctttgccggg cgcgccagca caatgggccg
     9541 cgatatttgc ccgttttggc gcgaaactgg aaaatgtcag cgtgggttgc tgcggcatgg
     9601 cagggactta cggacatgaa gcgaaaaacc atgaaaattc gctcgggatc tatgagttat
     9661 cctggcatca ggctatgcag cgactgccgc gtaaccgctg tctggcgacc ggatattcct
     9721 gccgtagcca ggtaaaacgg gttgaaggca cgggggtacg ccatcctgtg caggctttac
     9781 tggagattat taaatgatat ggaaacggaa aatcaccctg gaagcactga atgctatggg
     9841 tgaaggaaac atggtggggt tcctggatat tcgctttgaa catattggtg atgacaccct
     9901 tgaagcgaca atgccagtag actcgcggac aaagcagcct ttcgggttgc tgcatggagg
     9961 agcatccgtg gtactggccg aaagtatcgg ttccgttgcc ggttatttat gtaccgaagg
    10021 tgagcaaaaa gtggttggtc tggaaatcaa tgctaatcac gtccgctcgg cacgagaagg
    10081 gcgggtgcgc ggcatatgta aaccgttgca tctcggttcg cgtcaccagg tctggcagat
    10141 tgaaatcttc gatgagaaag ggcgtttgtg ctgttcgtca cgattgacga ccgccatttt
    10201 gtgacgttgt caccagaaaa gtgtgacgca tattgctttt ggtcattatc tgcacagtaa
    10261 acggtggtat actgtgcagg tcttgtctaa aagcgaggat gctatgtcta ctcaacttga
    10321 tcccacccag ctggccattg aatttttacg ccgtgaccag agcaatcttt cacctgccca
    10381 atatctcaaa cgtctgaaac aactagaact tgaatttgct gacctgctta ctctctcttc
    10441 tgctgaatta aaagaagaaa tctacttcgc ctggcgtctg ggcgtgcatt gatcctgact
    10501 taatgccgca agttcgccaa atcgacctgg attaacctcc gtccaggttc gatgaccttc
    10561 ttaatactgc ttatctaccg cctgatattg ttcaaaaata atactaatcg cagtttgtgt
    10621 taaaacggcg ggttagcttt atgagattat tcttatcgcc ccttcaagag ctaagccact
    10681 gagagtgccg gagataagcg ccggatgggg tagaaaccct taagcctgtg tcgcacagac
    10741 ttaagggttt tcttatttct gaacgctgca cttttttacc tatctcttaa atagtcattt
    10801 tcaatacaac tttttctttt tcatcacagc cttaatgctc cttagataat aaccattatc
    10861 taacaatgag atacctaatt cttagcgtgc ctgttaaccc acacatcagg ggctatgctt
    10921 attaaaagca ggcaaaaaga atgttttacg gtaaagcccc tgcgtttgct gggttgaact
    10981 gataatcatt atcactaaca tgctgttata cgctgaaagc gatgaagtga ggtaaataga
    11041 tggacatgca ttcaggaacc tttaacccac aggatttcgc ctggcaaggc ttaacgctga
    11101 cacccgcagc ggcgatacac atccgcgagc tggtggcaaa gcagccaggt atggtcggcg
    11161 tgcgcttagg tgtgaagcaa acgggctgcg cgggctttgg ctatgtgctc gacagtgtta
    11221 gcgagccgga caaagacgat ctgctgtttg aacacgacgg cgcgaagctg tttgtcccgc
    11281 tgcaatcgat gccgtttatt gatggcacgg aagtcgattt cgttcgtgaa ggacttaatc
    11341 agatattcaa atttcacaac cctaaagccc agaatgaatg tggctgtggc gaaagcttta
    11401 gggtataggc ggtactatgt ctcgtaatac tgaagcaact gacgatgtca aaacctggac
    11461 cggcggcccg ctgaactata aagaaggatt cttcacccag ttagccaccg atgagctggc
    11521 aaaggggata aacgaagagg tggtgcgcgc aatttcggcg aagcgtaatg agccggagtg
    11581 gatgctggag tttcgtctaa acgcctatcg cgcatggctg gagatggaag aaccgcactg
    11641 gttgaaagcg cactacgaca agctgaatta tcaggattac agctactact cagcaccatc
    11701 gtgcggtaat tgtgacgaca cttgcgcgtc tgaacctggc gcggtgcagc aaactggcgc
    11761 gaacgccttt ttaagtaaag aggtggaggc ggcgtttgag cagttgggcg ttcccgtgcg
    11821 ggaaggcaaa gaggtggcgg tggatgccat tttcgactct gtttcggttg ccaccactta
    11881 tcgcgaaaaa ctggcggagc agggaattat tttctgttcc tttggtgagg cgatccacga
    11941 tcacccggaa ctggtgcgta aatatctcgg caccgtggtg ccggggaatg acaacttctt
    12001 tgcagcactt aatgcggcgg tagcctctga cggtacgttt atttatgtgc ctaaaggcgt
    12061 gcgctgcccg atggaacttt ccacctattt tcgcattaac gcggaaaaaa ccgggcagtt
    12121 tgagcgcaca attctggtgg ccgacgaaga cagctacgtc agctacattg aaggctgttc
    12181 cgctccggtg cgtgacagct atcagttgca cgcggcagtg gtggaagtca tcatccataa
    12241 aaacgccgag gtgaaatatt ccacggtaca aaactggttc cctggcgata acaacaccgg
    12301 cggtattctc aacttcgtca ccaagcgtgc tttgtgcgaa ggcgaaaaca gcaaaatgtc
    12361 atggacgcaa tcagaaaccg ggtcagcgat tacgtggaaa tatcccagct gcattttgcg
    12421 cggcgataac tccattggtg agttttactc agtggcgctg accagcggtc atcagcaagc
    12481 ggataccggc accaagatga tccacatcgg taaaaacacc aaatcgacca ttatctcgaa
    12541 agggatctct gccggacata gtcagaacag ttatcgcggc ttagtgaaaa tcatgccgac
    12601 ggcaaccaat gcgcgcaatt tcactcagtg cgactcaatg ctgattggcg ctaattgtgg
    12661 ggcgcatacc ttcccgtatg tcgaatgtcg caataacagc gcacaactgg agcacgaggc
    12721 aacgacatca cgtattggtg aagatcaact gttttactgc ctgcaacgcg ggatcagcga
    12781 agaagacgcc atctcgatga ttgttaacgg tttctgcaaa gacgtgttct cagaactgcc
    12841 gctggaattt gccgttgaag cacaaaaact cctcgccatc agtcttgaac acagcgtcgg
    12901 ataaggaata aacatgttaa gtattaaaga tttacacgtc agcgtggaag ataaagctat
    12961 cctgcgtgga ttaagcctcg acgttcgtcc cggcgaagtt cacgccatta tggggccaaa
    13021 cggttcgggc aaaagtacct tatcggcaac gcttgccggg cgcgaagatt atgaagtgac
    13081 gggcggcacg gttgagttca aaggcaaaga tttgcttgca ctgtcgccgg aagatcgtgc
    13141 aggcgaaggc atctttatgg ccttccagta tccggtggag attccaggcg tcagtaacca
    13201 gtttttcctg caaacggcac ttaatgcagt gcgcagctat cgcggccagg aaacgctcga
    13261 ccgctttgat tttcaggatt tgatggaaga gaaaatcgct ctcctgaaga tgccggaaga
    13321 tttattaacc cgttcggtaa acgttggttt ttccggcggc gagaaaaagc gcaacgatat
    13381 tttgcaaatg gcggtgctgg aaccggagtt atgcattctt gatgagtcgg actccgggct
    13441 ggatattgac gcattaaaag tggtcgccga tggcgtgaac tcgctgcgtg atggcaagcg
    13501 ctcattcatc attgttacgc actaccaacg cattctcgac tacatcaagc ctgattacgt
    13561 tcatgtgcta tatcagggac gaattgtgaa atccggcgat ttcacgttgg tcaaacaact
    13621 ggaggagcag ggttatggct ggcttaccga acagcagtaa tgcgctgcaa cagtggcatc
    13681 acttgtttga agctgaaggg acaaaacgct caccgcaagc acagcagcat ttacaacaat
    13741 tgctgcgtac cggactgccg acacgtaaac atgaaaactg gaaatatacg ccgctggaag
    13801 ggctgaccaa tagtcagttt gtcagcattg cgggagatat atccccacag cagcgtgatg
    13861 ccttagcgtt aacgttagac gccgtgcggc tggtgtttgt cgatggacgt tacgtgcccg
    13921 cgctgagcga tgcaattgaa ggcagcggat atgaagtgag cattaacgac gaccgtcagg
    13981 ggttacccga cgctattcag gcggaagtgt ttctgcattt gacggaaagc ctggcacaaa
    14041 gcgtgacgca tatcgccgtg aagcgcggtc aacggccggc aaagccattg ctgttaatgc
    14101 atatcaccca gggcgtggca ggtgaagagg tgaacactgc ccattaccgg catcatctgg
    14161 atctggcgga aggtgccgaa gcaacggtga tcgaacattt tgtcagcctt aatgatgctc
    14221 gtcattttac cggggcacgg ttcactatca atgtcgcagc gaacgcccac ttgcagcata
    14281 tcaagctggc gtttgaaaac ccggtcagtc accactttgc ccataacgat ttgttgctgg
    14341 ctgacgatgc caccgcattt agccatagtt tcctgctggg tggcgcagtg ttacgacaca
    14401 acaccagtac gcaactcaat ggcgaaaaca gcacgctgcg gatcaatagc ctggcgatgc
    14461 cggtgaaaaa cgaggtgtgt gatacccgta cctggctgga acacaataaa ggtttttgta
    14521 acagccgaca gttgcacaaa actatcgtca gcgacaaagg ccgcgcggta tttaacggtt
    14581 tgatcaacgt cgcgcagcac gccatcaaaa cggatggtca gatgaccaac aataatctgc
    14641 tgatgggcaa actggcggaa gtggatacaa aaccgcagct ggaaatctat gcagatgatg
    14701 tgaaatgcag ccacggcgcg acggtggggc gtattgatga tgaacagatg ttctatctgc
    14761 gctcgcgcgg gatcaatcag caggatgccc agcagatgat catttacgcc tttgctgccg
    14821 aactgacgga agcactgcgt gatgaggggc ttaaacagca ggtgctggcc cgaatcggtc
    14881 aacggctgcc aggaggtgca agatgacttt ttccgtcgac aaagtgcggg ccgactttcc
    14941 ggtgctttcg cgtgaggtaa acggtttgcc gctggcttat ctcgacagcg ccgccagtgc
    15001 acagaaaccg agccaggtga ttgacgccga ggccgagttt tatcgtcatg gctacgcggc
    15061 ggtgcatcgc ggtattcata ccttaagcgc ccaggcgacc gagaaaatgg agaacgtgcg
    15121 caagcgggca tcgctgttta ttaatgcccg ttcggcggaa gagctggtgt ttgtccgcgg
    15181 cacgacggaa gggatcaatc tggtcgccaa tagctggggc aacagcaatg tgcgggcggg
    15241 cgataacatc atcatcagtc agatggagca ccacgctaac attgtcccct ggcagatgct
    15301 ttgcgcacgc gttggcgcag agctgcgtgt gatccctctc aaccccgacg gtacgctgca
    15361 actggagacg ctgcctacgc tgtttgatga gaaaactcgc ctgctggcaa ttactcatgt
    15421 ctccaacgtg cttggcacag aaaatccact ggcggaaatg atcacgcttg cgcaccagca
    15481 tggcgcaaaa gtgctggtgg atggcgctca ggcggtgatg catcatccgg tggatgttca
    15541 ggcgctggat tgcgactttt acgtgttctc cgggcataaa ctgtatggcc ccaccggaat
    15601 tggcattctt tatgtgaaag aagccttgtt gcaggagatg ccgccgtggg aagggggcgg
    15661 ttctatgatc gccaccgtca gcctgagtga aggcactacc tggaccaaag caccatggcg
    15721 gtttgaagcc ggtacaccca ataccggggg catcattggt cttggcgcgg cgctggagta
    15781 tgtttcggcg ctggggctta atagcatagc cgagtatgaa cagaatctga tgcattatgc
    15841 gctatcacag ctggaatctg taccggatct cactctctat ggccaacaaa acaggcttgg
    15901 cgttattgct tttaatctcg gtaaacacca cgcctatgat gttggcagtt ttctcgataa
    15961 ttacggcatt gctgtgcgta ccggacatca ctgcgctatg ccattaatgg cctattacaa
    16021 cgtccctgcg atgtgtcggg cgtcgctggc catgtataac acccatgaag aagtggagcg
    16081 tctggtgacc ggcctgcaac gtattcaccg tctgctggga taacagggag gcactatggc
    16141 tttattgccg gataaagaaa aattgctgcg taatttttta cgctgcgcca actgggaaga
    16201 gaaatatctc tacattattg agctgggcca gcgtctgcca gaattacgcg ccgaagacgg
    16261 aagcccacaa aatagcattc agggctgcca gagtcaggtg tggattgtca tgcgccagaa
    16321 tgcgcaggga attattgaat tgcagggcga cagcgatgcg gcgattgtga aagggcttat
    16381 tgcggtcgtc tttattctct acgatcagat gacgccgcag gatattgtca atttcgatgt
    16441 gcgtccgtgg tttgaaaaaa tggcgctcac ccaacatctc accccatctc gttcacaagg
    16501 tctggaagcg atgattcgcg caattcgcgc caaagccgct gcacttagct aaactaaagg
    16561 gacagctttc atccggttgg ctcacattgc cggatggtca atgtttacac tgaccggcac
    16621 aggagtgttt tggtttcagg tgaacataag caccatccga cgtttcagcg tgagtctccg
    16681 gcaaatacag gaggtttaca atgaaacgcg cgtctttgct tacactcacg cttatcggcg
    16741 cttttagcgc catccaggct gcctgggcgg ttgattatcc gctaccacca accggaagcc
    16801 gactggttgg gcaaaatcaa acgtatacgg tgcaagaagg ggataaaaac cttcaggcta
    16861 ttgcccgacg ttttgatact gcagcaatgt tgatccttga agccaataac actatcgccc
    16921 cggtgccaaa acctggtacg acgataacta ttccttcaca actgttatta cctgatgcac
    16981 cgcgtcaggg gattatcgtt aaccttgcag agctgcgcct ttattattat ccgccgggag
    17041 aaaatattgt gcaggtttat ccaataggta ttggattgca ggggctggaa acgccggtga
    17101 tggaaacgcg tgttgggcag aaaatcccta acccaacctg gacgcctacg gcaggcattc
    17161 gtcagcgttc gctggagcgt ggcattaaat taccgccagt cgttcctgcc ggaccaaata
    17221 acccgctagg acgttacgca ctgcgcctcg cgcatggtaa tggcgaatac ctcattcatg
    17281 gcaccagtgc gccggacagc gttggtttgc gcgtcagttc agggtgtatt cgcatgaatg
    17341 caccggatat taaagccttg ttctccagcg tgcggacggg aacgccggtg aaagtgatca
    17401 acgaaccggt gaaatattcc gtggaaccta acgggatgcg ttatgttgaa gtacatcgac
    17461 cactatcagc agaagaacag caaaacgttc agacaatgcc atacacactg ccagcaggct
    17521 ttacgcaatt taaagacaat aaggctgtag atcagaagtt agtcgataaa gcgttgtatc
    17581 gtcgggcagg gtatccggtt tcggtgagca gtggagcaac tcccgcagcc agcaatgcgc
    17641 cttcagtaga gtcagcgcag aatggtgaac cagagcaagg gaatatgtta cgcgcgacgc
    17701 agtagtggta aatagcagac aaaaaaatgg cgcacaatgt gcgccatttt tcacttcaca
    17761 ggtactatta cttgcggtat ttagtagcca tgttgtccag acgctggtta gcacgagctg
    17821 cgtcatcttt agcagcctga acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt
    17881 caactttagc gttcagagtc tgaacgtcag aagacagctg atcgatttta gcgttgctgg
    17941 agcaacctgc cagcagagta gaacccagga ttaccgcgcc cagtaccagt ttagtagctt
    18001 tcattattaa taccctctag attgagttaa tctccatgta gcgttacaag tattacacaa
    18061 agttttttat gttgagaata tttttttgat gggaaggcac ttatttttga tcgttcgctc
    18121 aaagaagcat cgaacgctgg gttttagata aaaaaattga gagaaaacag gatgcttcca
    18181 tcggattcat cttaaataaa gtgagattat atagtgaaat ccgatttgat tttttattgc
    18241 ttctggttat cgattaaata aaaaaagcgc ccatcagggc gcttcgatat acaaattaat
    18301 tcacaaaagc aatattacag gacgtgaaca gatgcggtgt tagtagtgcc gctcggtacc
    18361 agtgcaccag aaaccataac tacgacgtca cctttgtgtg ccagaccgct ctgcagagcc
    18421 agttctttac ccagacggta gaaatcatca gtagaagtga tctctttaac cagctgcggc
    18481 acaacgcctt tgctcagtac caactgatga gccgtttttt cgttggtggt cagtgccagg
    18541 atggtggcat ccgggaagta tttacgtact gcgcgagcag atttaccgcc ctgggtagca
    18601 accacgatca gcggagcatc cagtttttca gcagtttcaa cggcaccacg gcataccgct
    18661 tcggtgatgc gcagtttacg gttgtcattg ttgaactcga gacggctgtt catcacgcgg
    18721 tcggtacgtt cgcagatggt cgccatgata gaaaccgctt ccagcgggta tttacctttt
    18781 gcggattcac cagacagcat cactgcgtca gtaccgtcga ggatggcgtt tgcaacgtca
    18841 ccggcttctg cgcgagtcgg gcgtgggttt ttgatcatgg aatccagcat ctgggtcgca
    18901 gtgataacga ctttacgtgc acggatacat ttttcgatca tcatcttctg ggcgaagata
    18961 acttcttcta ccgggatttc tacacccagg tcgccacgcg caaccatgat gccgtcagag
    19021 gcttcgagga tttcgtcgaa gttgttgagg ccttcctggt tttcgatttt ggagatgatg
    19081 tggatgtttt cgccgccgtg cgctttcagg tgctcacgga tttcgataac gtcagaacgc
    19141 ttacgaataa aggaagcagc aacaaagtct acgccttttt cgcaaccaaa gatcaggtcc
    19201 tgtttgtctt tttcagccag tgctggcaga gcaatggaaa cgccaggcag gttcacacct
    19261 ttgttttcgc ccaggtcacc gttgttcagc actttacaga taactttgtt accttcaatg
    19321 gcggtaactt ccataccgat cagaccatcg tcaaccagta cggtgttgcc aacagacagg
    19381 tcagtagtga aaccttcata cgttaccgca accatttcgc tgttgccgat aacagattta
    19441 tcagtggtga aagtaaaggt ctgaccagct ttcagagaaa catcgttacc gccttccagt
    19501 ttcatggtgc ggatttccgg acctttggta tcaagcagga tagcggcggt tttaccagtt
    19561 ttgctcatca cgttgcgcag attctgaatg cgctgaccgt gttctgcata gtcaccatga
    19621 gagaagttca gacgcataac gttcatgcca gcgtccagca ttttagctaa catctcttca
    19681 gattcggttt tcggtccgat ggtgcaaaca attttggtct ttttcatgac agtcttagtc
    19741 tttaagttga gaaggatggg agaaacttgc tttctgggcg cactgccgga aagcgaatcc
    19801 ggtattacga aagtggtggt gcctcgctct aaggataggt gacat
//
LOCUS       DAJTYH010000077        19329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTYH010000077 DAJTYH010000000
VERSION     DAJTYH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(143..1576)
                     /gene="hldE"
                     /locus_tag="OO609_002729"
     CDS             complement(143..1576)
                     /gene="hldE"
                     /locus_tag="OO609_002729"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6634089.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1624..4464)
                     /gene="glnE"
                     /locus_tag="OO609_002730"
     CDS             complement(1624..4464)
                     /gene="glnE"
                     /locus_tag="OO609_002730"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6634090.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4487..5788)
                     /gene="ygiF"
                     /locus_tag="OO609_002731"
     CDS             complement(4487..5788)
                     /gene="ygiF"
                     /locus_tag="OO609_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6634091.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6030..6650
                     /locus_tag="OO609_002732"
     CDS             6030..6650
                     /locus_tag="OO609_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6634092.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6714..7952
                     /gene="cca"
                     /locus_tag="OO609_002733"
     CDS             6714..7952
                     /gene="cca"
                     /locus_tag="OO609_002733"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6634093.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8115..8936)
                     /gene="bacA"
                     /locus_tag="OO609_002734"
     CDS             complement(8115..8936)
                     /gene="bacA"
                     /locus_tag="OO609_002734"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6634094.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9027..9395)
                     /gene="folB"
                     /locus_tag="OO609_002735"
     CDS             complement(9027..9395)
                     /gene="folB"
                     /locus_tag="OO609_002735"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6634095.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9500..10117
                     /gene="plsY"
                     /locus_tag="OO609_002736"
     CDS             9500..10117
                     /gene="plsY"
                     /locus_tag="OO609_002736"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6634096.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10130..11061)
                     /gene="ttdR"
                     /locus_tag="OO609_002737"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10130..11061)
                     /gene="ttdR"
                     /locus_tag="OO609_002737"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11268..12111
                     /gene="ttdA"
                     /locus_tag="OO609_002738"
                     /pseudo
     CDS             11268..12111
                     /gene="ttdA"
                     /locus_tag="OO609_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12108..12713
                     /gene="ttdB"
                     /locus_tag="OO609_002739"
     CDS             12108..12713
                     /gene="ttdB"
                     /locus_tag="OO609_002739"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6634097.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12761..14224
                     /gene="ttdT"
                     /locus_tag="OO609_002740"
     CDS             12761..14224
                     /gene="ttdT"
                     /locus_tag="OO609_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6634098.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14267..15280)
                     /gene="tsaD"
                     /locus_tag="OO609_002741"
     CDS             complement(14267..15280)
                     /gene="tsaD"
                     /locus_tag="OO609_002741"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6634099.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15518..15733
                     /gene="rpsU"
                     /locus_tag="OO609_002742"
     CDS             15518..15733
                     /gene="rpsU"
                     /locus_tag="OO609_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6634100.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15844..17589
                     /gene="dnaG"
                     /locus_tag="OO609_002743"
     CDS             15844..17589
                     /gene="dnaG"
                     /locus_tag="OO609_002743"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6634101.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17784..>19329
                     /gene="rpoD"
                     /locus_tag="OO609_002744"
     CDS             17784..>19329
                     /gene="rpoD"
                     /locus_tag="OO609_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6634102.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDE"
BASE COUNT         4603 a         5398 c         4927 g         4401 t
ORIGIN      
        1 tgttaagttt cccgacttac taacaactga tcagaggggg gagaaatcct cccttaccct
       61 tgttccttta ctttaggttg aaaaaacaac agcgtcaata ggcctgcctg ctacgaagcg
      121 agatctgtga accgctttcc ggttagcctt ttttatcctg ttggatcttc ttgatgatgt
      181 tagtggtcga gcaaccgtct tcgaagttga gcaccagcac ttcgccgccg ttggcccaga
      241 cttctttact cccggcgatc tcttctggtt tatagtcgcc gcccttcacc agtaaatctg
      301 gcaggatccc ggcgatcaag cgctgcggcg tgtcttcttc aaacgacacc acccagtcga
      361 ccgcttccag tgcgcccagc acaatcatac gctgttcgag cgggttaacg gggcgagaat
      421 cccctttcag ccgtttggtg gaggcatcgc tgttcacagc gacaatcaag cggtcaccca
      481 gtttgcgggc atttgccaga tagaagacgt gtccggcgtg caggatgtca aagacgccat
      541 tggtcatcac cactttttca ccgcgtttac gcgctgccgc tacggccagc ttcagttcct
      601 cttcggtcat cacgccaaag ccggtatctg cgcgcccacg cactgcattt tccagctcga
      661 tcggcgaaac cgtggaggtt cccagtttgc cgaccaccac gccagccgcc gcattggcaa
      721 agaagcaggc ttcttccagc gaattacccg ctgccagcgt tgccgccagg acgccaatca
      781 ccgtgtcgcc cgcaccggta acgtcataca cttcctgcgc ctgggttggc atatgcagcg
      841 gcgctttacc cggttgcagc agcgacatac cctgttcgga acgggtcact aacagagccg
      901 agagttcgta atcggcaatc agtttcatgc cgcgctcaac aatctcttct tcggtcttac
      961 atttaccgac aacagcttca aattccgaga gattcggtgt cagcagcgta gcgccgcggt
     1021 agcgctcaaa atcggtacct tttggatcaa tcagcaccgg aacgcccgct ttacgcgcca
     1081 gttggatcat ctgctgtacg cttgccagcg cacctttggc gtagtcagaa agcaccagcg
     1141 cgccaatcga actcagcgcc tgattaatcc gctcgtgcag cggctgcgga tcaacacctt
     1201 cgaaaccttc ttcaaaatcc agacggatca gctgttggtt gcgggaaagt acccgtaatt
     1261 tggtgatggt cggatgcgtc ggtacagaaa cgaagtcgca tttgacgttg acgtcggcca
     1321 gagatttact cagcgcgcgc gctgcatcgt caatgcccgt caacccgacc aggcgtgcat
     1381 tagcaccgag agaagcgata ttcatcgcca cgttagccgc gccgcccgga cgttcttcga
     1441 tggtattcac tttaaccacg ggcaccggcg cttccggcga gatacgactg gtggggccgt
     1501 accagtaacg atccagcatc acatcaccaa ccaccatcac tcctgcacgt tcaaactctg
     1561 gcagcgttac tttcattcct gtctcctgag agattcaaaa tttgcgcgcg ataataccat
     1621 acttcattct tccaccagcc acttctgcca gcttgcccgc accagatcac gctctgcggt
     1681 gaagcaatcc tccgacacat ggcccggcaa ttcctgtaat gccagatgat gaagttcatc
     1741 gcgcagcgtc gtgtaagcac gggtcagcgc catcgcttcc tgctcttcca taatgtcgtt
     1801 ttgcgccagc agctccagaa tacgcacgtt gtctgaccag cgcgttaact tcggtttttc
     1861 atgagcgtag cgcaacacca gatattgggt aataaattcg atatcggtaa ttccgccttc
     1921 atcggctttg atatcaaagc gattacgatg tttattgccg agatgagcgc gcattttctc
     1981 gcgcatttcc cgcacttccg tttgcagagt tttaccttct cgcggcagtg tcataatctc
     2041 acgacgcacc gcgtcaaagt gcgcagtgag ctgcggatcg ccgtacacta cacgcgcacg
     2101 caccagcgcc tgatgttccc acgtccaggc ctcgtttttc tgataatcgg caaatgcttc
     2161 tgcggatgtc accagcattc ccgccgcccc ggacggacgc agtcgagcat ccacttcata
     2221 caaaatgccg gaagaggtac gcgtactgaa cagatgcata atgcgttgcg ccagacgcag
     2281 ataaaactgc cgcccgtcga tttcccgctc gccgtcagtc atcgcatcca ttgggcaatc
     2341 atggaggaag ataaggtcaa gatcggaact gtagcctaac tcccagccgc ccagcttgcc
     2401 gtagccgacc accgcaaaac cgcgcccttc acgttcgttg aggtgatttg gcttaccgta
     2461 gcgggccacc atttgacccc acgcctgctg aacgacggca tctatcatag cttctgccag
     2521 ccaggttaag tgatcgctca ctttcattac cggtagcgta ccggcgatat ccgctgccgc
     2581 aatgcgtaac agctgcgcct gtttgaactg acgcagcgcc tcaagctgtt gttcttcgtc
     2641 atcttccggc acgcgcagca aatactggcg cagttcatca cggtaggcat cggtcgccgt
     2701 cggctgataa agggtatttg gatcgagcaa ttcatccagc aataatggat aacgcgctag
     2761 ctggctggca atcatcggcg atgcggcaca cagagaaatc aaatgtttaa gcgccgcagg
     2821 gaattcactg agcaattcta aataggttgt gcgggtaaca atccccacca gcaaggcggt
     2881 aatgcgcgac agcgtgacag cagcgtcttc acgcgcacag acatcactta gcagatgtgg
     2941 catcagatgg tcgagcacct gacgtcctcg cggcccgata gtacgcttat ccagctcttt
     3001 gcggaaatca gcaatcatcg ttaggacctg tttgcgatca tcctcgctaa gatgcgccag
     3061 cactggcgta gtgtcatctt cctgcaacgc atcctgccac agctcacgcc actgttccga
     3121 cagcgactct tcctgagttt cactttcgtc gtcgccaatc aattcattaa acacccggcg
     3181 cacattggtc atatgtgcgg tcagcgcccc ggtcagttgc ggccagtcag caaagtccat
     3241 cgcccacgcc agccgcgcac ggttaagctc atcagaagga agcgtttggg tttgttcgtc
     3301 gttaatgctt tgcagcaggt tttccagacg ccgcaggaac agatatgcca ctcgcaattg
     3361 ttcagcatcg ttttcagaaa gcagatgcag cgcggcaatg gcgctgagcg ttggcagtaa
     3421 agagcgcgac tgcagcaacg gttcgcgccc accgcgaatg agctggaaca cctgaacgat
     3481 aaattcaatt tcgcgaatgc cgcctgcgcc gagtttgata ttgtcggtca aaccacgtcg
     3541 acgcacttca cgggcaatca tccctttcat gttgcgcagc gactgaatca cgctgaaatc
     3601 gatgtaacga cggaaaacaa acgggcgcag catcgcacgc aactcgttag catagacgcc
     3661 ttcgctatcg cccataatcc gcgctttgac catcgcgtaa cgctcccagt cgcgtccctg
     3721 ctcctggtaa taatcctcca acgcggcaaa gctcagcacc agcgggccac tttcgccaaa
     3781 cggacgcagt cgcatatcca cgcgatagac gaagccatcc tgcgttggtt gatccagcac
     3841 tttaatcagc cgctgcccca tgcgggtaaa aaactgcgcg ttatccagtt cccggcgtcc
     3901 gccctgcgta caaccatgtt ccggccaggc aaaaatcaga tcgatatcag aggagaaatt
     3961 cagttccccg ccgcccagct tacccatgcc taagatcagc agcggttgcg cttcgccctg
     4021 cgcattgcac ggcgttcccc actcgcggca gcaggcgtca tacagccagt cacgcgccgc
     4081 aacaatcagc gtctccgcca gataactgag ctgctgcaat atgctctctt cagtaaccag
     4141 tgccagcgtt tgcgcccatg cgatgcgcac cataatgcgc cgccggaata gccgcagctc
     4201 gcgcattaac ccggcttcgt cgctcacatt actgagcgcc tcctgcaacc acacgctgta
     4261 atgttgccat tcgtcagcct gtggcggttg gctttccagt tccgtcagcc actctggatg
     4321 cgcaatcacg ctgtcctgca caaaatcact gaaagtaagt actgacttcg cctgtgcact
     4381 aagtgattcc tcggctaaaa gctctggcag ccgctcaaca acggtctgcc agtactgctg
     4441 taacggtgaa gagagcggct tcataagcga ttttatcctt gcctgattaa cgttttccgc
     4501 tgtgcaacca gaacggttcc tgattgtttg cctcattacg gaaatgttca atttcaatgc
     4561 gttgcccggt cgcaatagcg tgacgcagcc cctgccagtt ctccagccag gcttgcgcga
     4621 cgacaggatc atagtaaccc gccagcaaca gtattgagtc aatatcacgc atcaggcgtg
     4681 gcaactggtc acggtagcga tcgcctaacg gctggcagaa aacgcttttc agttcagcgg
     4741 catggcggga aagatggata tcggcaaagc gtttgaagga gtcgctaatt ttgccctgcg
     4801 ctttggcatc taaaaacggc tgccatgctt tgcttaccag ccattcggtc aacgccagct
     4861 tcgccattgc cgtttcggta gagtagaccg ccgtcacggc agaaaccgca gaagcgatgg
     4921 tcgcttcgca ttgagtcagc aaatcacgta agtgagtact cgctttacgc ggcacaatgc
     4981 caccgaacag catcagtgta tgacggacta ggctaatggc tgccagcacc tgctctttcg
     5041 ccgcatcgtt gccgcgtacc cacagttctt catgatactg ccattgcgct aacaccagct
     5101 cgagcgccgc ttccagcccc tgttccacat cggcttttgc cgcaacatgc aaaatggtgg
     5161 tcggtttgat ttcacgcgcc ggattgccct gcgccagatg atagccacgc gccgctttgc
     5221 tcaggctgcc ctggcgtaat ccggtttgcg ataccagttg gttcgccagt ttcagcaccg
     5281 cgcgcgtgtc gccgctaagc agctccagtt ccagctcgca gataggctca gcaaattcac
     5341 ccgctttcac ttccccctgg tcgagagcga tttcaattcg gctgccatcg atctctacta
     5401 gccatttttc gcgataaaaa tcggtgctaa acagcgactg cacgcgggaa gcgagatcgg
     5461 caggcaattc gccgttcggc cagatttccg tcggtaactg cgccaggtcg agcgtcggtt
     5521 cgctcaacgc cacgttatat tccgggcgct ggtgtaagcc gcctgtcact cttcctgcaa
     5581 ctttcatggt catctcatag cgaccgtttt cgccacgaat acgtaagccc atatcgtgcc
     5641 tacgcagcca gttatccggc gtttcgtagt aaatattcag caactgcacg gggtcatggt
     5701 gctcgccgcc cagcgtattg agatggtcac gcaacgcctc aacggcactg tgattaacaa
     5761 taaactttaa ttcgatttcc tgagccatgg cctttactta tcggttatgt cacataagtg
     5821 acgatgaacg gcgaacttaa tgcgatcttt tttgtcagta gatagtattt tgcgccaaat
     5881 tgccatgcaa cgggcaattt gacgggcgta aaagtttgaa gcagtggcaa gacctgacac
     5941 agaggattcc gattgatgtc gaatgctttg cgtagcgaca ctgatgccac tactatcgtt
     6001 ccactttcca tgacaataac gacagcctga tgccaaaatt acgcctgatc ggattaactt
     6061 tactcgcact tagcgcgact gccgtctcac acgctgaaga aacgcgctat gtttctgacg
     6121 aactgaatac ctgggtccgt agcggtccgg gagatcatta tcgcctcgtg ggcacggtta
     6181 acgccggcga ggaagtgacc ttattacaaa ctgacgccaa caccaattat gcccaggtga
     6241 aagacagctc tggccgtacc gcctggatcc cgttgaaaca acttagcact gagccaagcc
     6301 tgcgctcccg tgtgccagat ctggaaaatc aggtcaaaac cctgaccgat aaactcacca
     6361 atatcgataa cacctggaat cagcgcaccg cagaaatgca gcaaaaagtg gcgcagagcg
     6421 acagcgtgat caacgggtta aaagaagaaa atcagaaact gaaaaacgag ctgattgtcg
     6481 cgcagaaaaa ggtcgatgcc gccagcgtac agctggatga caaacagcgc accatcatca
     6541 tgcaatggtt tatgtatggc ggtggcgtgc tggggcttgg cttgctgctc ggtctggtac
     6601 tgccgcacct gatcccaagc cgcaaacgca aagatcgctg gatgaactaa atcgccttct
     6661 tcgttgcact gtcttattat taggaattgt gtatttgacg aagggatgat ggcgtgaaga
     6721 tttatctggt cggtggtgct gttcgggatg cattgttagg actatcggtc aaagacagag
     6781 attgggtggt ggtcggcagt acgccacagg agatgctcga cgcgggctac cagcaggtag
     6841 gccgcgattt tcctgtgttt ctgcatccgc aaacgcatga agagtatgcg ctggcacgta
     6901 ccgaacggaa atccggttcc ggttacaccg gttttacttg ctataccgca ccggatgtca
     6961 cgctggaaga tgatcttaag cgtcgcgatc tgaccattaa tgcgctggcc caggacgata
     7021 acggcgagat tatcgacccg tacaacggtc tgggcgatct gcaaaatcgt ctgttgcgcc
     7081 atgtttcccc cgcttttggc gaagatccgt tacgcgtact gcgcgtggcg cgttttgctg
     7141 cgcgttatgc ccacctcggt tttcgtattg ccgatgaaac tctgacgctg atgcgcgaga
     7201 tgacccatgc gggtgaactg gaacacctga cgcctgaacg ggtatggaaa gagacggaaa
     7261 gcgccctcac cacccgcaat ccccaggtgt tcttccaggt actgcgcgat tgcggcgcac
     7321 ttcgcgtttt attcccggaa attgacgcac tgtttggcgt tccggcacct gccaggtggc
     7381 atccggaaat cgacacgggt attcatacct taatgacgct ctcaatggcg gcgatgctga
     7441 gtccgcaggt tgatgtccgt ttcgcaactt tatgccacga tctcggtaaa ggactgacgc
     7501 cgccagaact ttggccgcgt catcatgggc atggcccggc gggtgttaag ttagtggaac
     7561 aactatgcca gcgtctgcgc gtgccaaatg aaattcgcga tttagccaga ctggtggctg
     7621 agtttcacga tctcatccgc accttcccaa tgctgaaccc aaaaaccatc gtcaaattat
     7681 ttgattccat cgatgcctgg cgtaaaccgc agcgtatcga gcaactggcg ctgaccagcg
     7741 aggctgacgt gcgcggcaga accggttttg aatcagcgga ctacccgcaa ggccgttggt
     7801 tgcgcgaagg ctgggaagtg gcgcagtcag tgccgacaaa agcggtcgtt gaagcgggat
     7861 ttaaaggcgt ggagattcgc gaggagctga cccgacggcg gattgcggcg gtagccagct
     7921 ggaaggaaca acgttgccca aagcctgact gagtataaaa ctaaacgtta gcacgaaccg
     7981 tcccctcgcc ccttcgggga gagggttagg gtgaggggaa cagaccagca ccggtgcgaa
     8041 caatcacccc caccccggcc ctctccctac agagcgaggg agaaaacccg gcctgctccc
     8101 gaaccttttc tcaatcaaaa gaacacgaca tacaccgcag ccgccacaat aaagcgataa
     8161 atggcgaacg ggataaacga aatgcgctta atcaattgca ggaaggtttt aatcgctatc
     8221 agcgccacca caaaagcggt gataaaccca acggcaaaca tcgggatatc gccggttgtc
     8281 aggaagcccc agcttttgta gagatcgagc gccgttgcgc ccatcatcat cggtaccgcc
     8341 agcaggaacg agaactcgga agcagcgtaa cggctcacgc ccatcagcat cccacctgaa
     8401 atggtcgccc cggaacggga gaaacccggc cacagcgcca gacactggaa acagccaatc
     8461 ataaacgcct gacgataggt catatcatca agacccggcg cacgcggctc tttcggcttc
     8521 aggcattcgg cggcaatcag caacaaaccg ccaacgacca gcgcatacat cacatttatc
     8581 gggttaaaca atgacttaat cgtgtcgtgg aataatagcc ccagtaccac cgccggaatc
     8641 atccccagca aaatgtggat cagcgttaaa cgacctttgc tttcaccttc gtgctgcaac
     8701 gggcggccaa agtggatgcc aatcaggcca aacagacgcc gccagaacat cactactacc
     8761 gccagaattg atcctaactg gatcacaact tcaaaggttt tcgccgtgtc gccctcaaac
     8821 cccaacaagt gaccgacaat aatcatatgg cccgtgctgg ataccggcag aaattctgtc
     8881 aatccttcga ccacacccaa tattgccgct atcagcagcg agtgcatatc gctcatcaat
     8941 aaacccctaa attattaaaa tgtaccgctt gtccgaacta ctgcagtatg accaggttat
     9001 aaccgtttgg tttaacagct gtaaaattaa ttattttctt tcagattatt gccacgctca
     9061 atgattacgc caacattcgc cgcccgcgcc actgcgcctg gcttgctgag tttgatacgc
     9121 acccacggcg agttgaagcg tgctaacagc agctccgcca cctcttcagc cacgcgttcc
     9181 accagcgcaa aacgcgcccc ctcgacgtgg ctgaccaccg tttctgcaat gtcagcgtaa
     9241 ctgaggcaat ccgccacatc atcacttttc gccgctttac ggttatccca tgccatttcg
     9301 atatcgaaca ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc aatagtggtg
     9361 attaccgaaa gttgctctat aaatacaata tccatcacgt cctgcctgct ttttggctaa
     9421 ccggatacca cttccggcga aatgtgcgta ttatccacag attcatcgtt gaacacgaat
     9481 tttcaaaacg gaacagctta tgagtgcaat cgcgcctgga atgatcctca tcgcgtacct
     9541 ctgcggctcc atttccagtg ccattctggt ttgccgcttg tgtgggctgc ccgatccgcg
     9601 aaccagcggc tccggcaatc caggcgcaac caatgtgtta cgtatcggtg gcaagggagc
     9661 agccgtagca gtactgattt tcgacgttct gaaaggaatg ttgcctgtct ggggcgcgta
     9721 tgaattaggt gtcagcccct tctggctagg cttaattgcc atcgccgcct gtcttggaca
     9781 catctggccc gttttcttcg gttttaaagg aggaaaaggc gttgctaccg cttttggagc
     9841 aatcgcacca attagctggg atctcaccgg agtaatggcg ggaacctggt tactgaccgt
     9901 gctattgagc ggatactcgt cgctgggagc gattgtcagt gcactgattg ctccgtttta
     9961 tgtctggtgg tttaagccac aattcacctt cccggtttcg atgctctctt gcctgatcct
    10021 gctgcgtcat catgacaaca tccagcgtct gtggcgtcgt caggagacaa aaatctggac
    10081 gaaattcaaa agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc tacatgacct
    10141 gatagccttc atcgggcttg cccagccgtt gctggcacca tgccgccaga aattccgcgc
    10201 agacacgaat ttcatgctgc gatagagcag ctcccggtaa acagcccaga tattggcgct
    10261 ttgtgcatac tccggcaata cttgcaccag tttgccactc tccagaaacg gcaacacatc
    10321 ccactcggaa cgcagcataa tccctttgcc ctccagcgcc cattgcaaca ctatctcgcc
    10381 gctattggag gaaagatgcc cgctaacctt caccgatttt ttctcctgac cgttccccaa
    10441 ctcccatatt ccgtgggtca tatcgcgctc tttggtcacc agacagtcat gacgacttaa
    10501 ttcctgtaat gattgtggct gcggatattt ttgcagatac tcaggtgctg cgcataatat
    10561 tcttttattt ttcgttaaca gatgcgcaat ataataatca ggaatttcgt cattaatacg
    10621 aatatccaga tcaatattat cctgcactaa atcaatttgc cgatcgaaca attcaaaatg
    10681 cacctgtaac tcaggataat tgcgcataag ttcggtaatg gctggcgcaa tatggctgcg
    10741 cccaaaacca aaactgcaac caatacgaat catcccctcc gggcgcgttt tgatttgcgt
    10801 gacgtcatcg accagtcgct gatattgggt gaggatttcc agcgcatgtt cgtaacaacg
    10861 ttggccactt tcagtcagcg ccacgccgcg agcggagcgg ttcagcagcg ttgttgccag
    10921 cgtattttca aggatttgga tccgcttggt gacaaatgca ggcgtttgcc caagcgtcgc
    10981 cgctgcagca ctaaaactcc cgctgtggac gatttctacc agtacctgca agtctttggc
    11041 taaaggccag ctattcagca tcgttaatta tccgcagttg tgataagcgc agtgtatttc
    11101 gcaaaacatt gccattccct gctctggcgc aatattcatt cttattttcg cgttgattca
    11161 cgaactgtta attacatatt aacggtggca aaaaatataa aaccacattt ttagtgttag
    11221 tttgtggcgg tgaatttttc cagacaaata caaaaactgg agttgccatg atgagcgaaa
    11281 gtaataagca acaggcagtg aataagttga cagagattgt cgctaacttt accgccatga
    11341 tttctacccg aatgcctgat gacgtggtgg ataaactaaa acagctaaag gatgccgaaa
    11401 cgtcgtcgat ggggaaaatt atctaccata cgatgttcga caacatgcaa aaagcgattg
    11461 acctgaatcg tcctgcctgt caggacaccg gggagattat gttcttcgtt aaagtcggtt
    11521 cccgcttccc actgcttggc gagctgcaaa gcatactcaa acaagccgtg gaagaggcaa
    11581 ccgtcaaagc gccactacgt cacaatgcgg tagaaatttt tgacgaagta aacaccggca
    11641 aaaataccgg tagcggcgta ccgtgggtca cctgggacat catccccgac aatgacgatg
    11701 cggaaatcga agtttacatg gcaggcggcg gctgcacgct acctggccgc tcgaaagtgt
    11761 taatgccgtc agaaggctac gaaggcgtgg tgaaattcgt cttcgaaaat atctccaccc
    11821 tcgccgtaaa cgcctgtcca ccggtactgg tgggcgtggg catcgccacc tcggtggaaa
    11881 ccgccgccgt actctcgcgt aaagccattt tgcgcccgat tggcagccgc catcccaatc
    11941 caaaagcggc agaactggag ctacgcctgg aagaaggact caaccgtctg gggattggtc
    12001 cacaagggct gaccggcaac agttcagtga tgggcgcatc gtcgcggcac gctgctggtt
    12061 catgccgatc tcacctttga aaatctgtct cacacccgga gcgcgttatg aaaaagatcc
    12121 tgacaacccc gatcaaagct gaagatctgc aagatattcg cgtcggcgat gtgatctacc
    12181 tgaccggtac gctggtgacc tgccgcgacg tttgtcaccg ccgtttgatc gaactgaaac
    12241 gtccgatccc ttacgatctc aacggcaaag cgattttcca cgctggcccc atcgtgcgca
    12301 aaaacggcga caaatgggag atggtctccg tcggcccgac aaccagtatg cgtatggaaa
    12361 gttttgaacg tgaatttatt gagcagaccg gcgtaaaact ggtggttggc aaaggtggta
    12421 tggggccgct gaccgaagaa ggctgccaga aattcaaggc gttacatgtg attttcccag
    12481 caggctgcgc ggtgctggcg gcaacccagg tagaagagat tgaagaagtg cactggacag
    12541 aactcgggat gccggagtca ctgtgggtct gccgggtcaa agagttcggc ccgctgattg
    12601 tctctattga tacccacggc aacaacctga tagccgaaaa caaaaagctg ttcgccgaac
    12661 gccgcgatcc catcgtggaa gagatctgcg agcacgtcca ttacatcaaa taaccctccc
    12721 ggagaggctc acccctctcc tttttcgcag gcataacacg atgaaacctt ccactgaatg
    12781 gtggcgatat cttgcgccgc tggcggtcat cgccattatt gctctacttc cggttcccgc
    12841 agggctggag aaccatacct ggctctactt tgccgttttt actggcgtga tcgttggact
    12901 gatcctcgaa cccgtgccgg gtgccgtggt ggcgatggtg ggtatctcca tcatcgccat
    12961 actctctccc tggctgctgt tcagccccga acagctcgcc cagccaggct ttaagttcac
    13021 cgccaaatcc ctctcctggg cagtttctgg tttttctaat tcggttatct ggctcatttt
    13081 cgccgccttt atgtttggca caggctatga aaaaaccggg cttggacgcc gcattgcgct
    13141 gattctggtg aaaaagatgg ggcatcgcac gctgtttctc ggctatgcgg tgatgttctc
    13201 cgagctgatc ctggcacctg taacaccgtc caactcggcg cgtggtgcgg gtattatcta
    13261 tcccatcatc cgtaacctgc caccgctcta tcaatcacaa ccaaacgaca gcagttcgcg
    13321 cagcattggc tcgtacatca tgtggatggg gattgttgcc gactgcgtga ccagcgccat
    13381 tttcctgacg gcgatggcac ctaacttgct gttaattgga ctgatgaaaa gcgcatctca
    13441 cgccacgctg agttggggcg actggttcct cgggatgttg ccgctcagca ttttactggt
    13501 tctgctggtt ccctggctgg cttacgtgct gtacccgccg gtactgaagt ctggtgatca
    13561 ggtgccgcgc tgggcagaga cggaactgca ggcaatgggc ccgctctgtt cgcgtgaaaa
    13621 acggatgctg gggctgatgg taggcgcgct ggtgctgtgg attttcggcg gtgattatat
    13681 cgatgccgcg atggtcggtt acagcgtggt ggcgctgatg ctgcttctgc gcattatcag
    13741 ttgggacgac attgtcagta ataaagcggc atggaacgtt ttcttctggc tggcctcgct
    13801 tatcaccctc gcgaccggac tcaacaatac cggttttatt agctggtttg gcaaactgtt
    13861 agcaggcagc ttaagtggtt attcgccgac gatggtgatg gtgacgttga ttgtggtgtt
    13921 ttatctactg cgctactttt tcgccagcgc cacggcgtat acctgcgccc tcgcaccgat
    13981 gatgatcgcc gccgcgctgg cgatgccgga aatcccgctg ccggtgttct gcctgatggt
    14041 tggcgcggca attggtctgg ggagcattct tacaccatac gccaccggac ccagtccgat
    14101 ttactacggt agtggttatc tgccaacggc ggattactgg cgactggggg cgatttttgg
    14161 gctgatattc ctcgtgttgc tggtgattac cggcttactg tggatgcccg tggtgttgct
    14221 ttaagtgagg acggtgccgg aaattgctct ccggcaccca atagttttac gcagccggta
    14281 actccgccag cggccagcgc ggacgcacgc taacgccgag atccgccgtc gcgcctgctt
    14341 taaaccgcac cattccggca taggcgatca tcgcgccgtt atcagtacaa aattccggac
    14401 gcgcgtagaa cacttcgccg cggcgttttt tcatcatttc agccagcttc gcccgtaacg
    14461 tgcggttagc actcacgccg cccgccatga ccagtcgctt aaagcccgtc tgatccaacg
    14521 ctcgcttaca cttaatcatc aacgtatcga ccaccgcatc ttcaaaggcg cgggcgatat
    14581 cagcacgcgt ctggtcgtcg gtgccgttgt cacgaatcgt gttcgccgca aaggttttca
    14641 gaccagaaaa gctgaaatcc agccccggac ggtcggtcat cggacgcggg aaaacaaagc
    14701 gcccggcagt accctgagcc gccattttcg acagtaacgg tccgccagga taatccagcc
    14761 ccagcagctt cgcggtttta tcaaacgctt caccggcggc atcatcgata gactcgccga
    14821 gcagctcgta ctgaccaatg ccagtcacgc tgattaactg cgtatgaccg ccggacacca
    14881 gcagcgcgac aaacggaaat tccggcgggt tatcttccag catcggcgct aacagatgcc
    14941 cttccatatg gtgtaccggg attgccggaa cgttccaggc aaacgccaga gaacgcccca
    15001 cggttgcgcc aaccagcagt gcgccgacta atccagggcc tgcggtatag gccacagcat
    15061 caatgtcttt tgccgttaaa ccagactcct ttagcgccgc ctggatcaac ggtacggttt
    15121 tacgcacatg atcgcgggag gccagttcag gcacgacgcc gccgtagtca gcgtgcaatt
    15181 tcacctgact atacaattgg ttggctaaca aacctttttc atcgtcgtaa atggcgatgc
    15241 cggtttcatc gcaggaagtt tcaataccca gtacacgcat gacttgtttt acctcgcttt
    15301 attaccgcgc agtgtaggac caatgcgggt tgatgtaaaa ctttgttcgc ccctggagaa
    15361 agcctcgtgt atactcctca cccttataaa agtccctttc aaaaaaggcc gcggtgcttt
    15421 acaaagcagc agcaattgca gtaaaattcc gcaccatttt gaaataagct ggcgttgatg
    15481 ccagcggcaa accgaattaa tcaaaggtga gaggcacatg ccggtaatta aagtacgtga
    15541 aaacgagccg ttcgacgtag ctctgcgtcg cttcaagcgt tcctgcgaaa aagcaggtgt
    15601 tctggcggaa gttcgtcgtc gtgagttcta tgaaaaaccg actaccgaac gtaagcgcgc
    15661 taaagcttct gcagtgaaac gtcacgcgaa gaaactggct cgcgaaaacg cacgccgcac
    15721 tcgtctgtac taattccccg agagcgttgc tctccgatca gaccgagttg tagttgtaag
    15781 gccgtgcttc cgaaaggaat gcgcggctta ttttcgttta tgaattgcta aaaatcgggg
    15841 cctatggctg gacgaatccc acgcgtattc attaatgatc tgctggcacg cactgacatc
    15901 gtcgatctga tcgatgcccg tgtgaagctg aaaaagcagg gcaagaattt ccacgcgtgt
    15961 tgtccattcc acaacgagaa aaccccgtca ttcaccgtta acggtgagaa acagttttac
    16021 cactgctttg gatgtggcgc gcacggcaac gcgatcgact tcctgatgaa ctacgacaag
    16081 ctcgagttcg tcgaaacggt cgaagagctg gcagcaatgc acaatcttga agtgccattt
    16141 gaagcaggca gcggccccag ccagatcgag cgccatcaga ggcaaacgct ttatcagttg
    16201 atggacggtc tgaatacgtt ttaccaacaa tctttacaac aacctgttgc cacgtctgcg
    16261 cgccagtatc tggaaaaacg cggattaagc cacgaggtta tcgctcgctt tgcgattggt
    16321 tttgcgcccc ccggctggga caacgtcctg aagcggtttg gcggcaatcc agaaaatcgc
    16381 cagtcattga ttgatgcggg gatgttggtc actaacgatc agggacgcag ttacgatcgt
    16441 ttccgcgaac gggtgatgtt ccccattcgc gataaacgcg gtcgggtgat tggttttggc
    16501 gggcgcgtgc tgggcaacga tacccccaaa tacctgaact cgccggaaac agacattttc
    16561 cataaaggcc gccagcttta cggtctttat gaagcgcagc aggataacgc tgaacccaat
    16621 cgtctgcttg tggtcgaagg ctatatggac gtggtggcgc tggcgcaata cggcattaat
    16681 tacgccgttg cgtcgttagg tacgtcaacc accgccgatc acatacaact gttgttccgc
    16741 gcgaccaaca atgtcatttg ctgttatgac ggcgaccgtg caggccgcga tgccgcatgg
    16801 cgagcgctgg aaacggcgct gccttacatg acagacggcc gtcagctacg ctttatgttt
    16861 ttgccggatg gcgaagaccc tgacacgctg gtacgaaaag aaggtaaaga agcgtttgaa
    16921 gcgcggatgg agcaggcgat gccactctcc gcatttctgt ttaacagtct gatgccgcaa
    16981 gttgatctga gtacccctga cgggcgcgca cgtttgagta cgctggcact gccattgata
    17041 tcgcaagtgc cgggcgaaac gctgcgaata tatcttcgtc aggaattagg caacaaatta
    17101 ggcatacttg atgacagcca gcttgaacga ttaatgccaa aagcggcaga gagcggcgtt
    17161 tctcgccctg ttccgcagct aaaacgcacg accatgcgta tacttatagg gttgctggtg
    17221 caaaatccag aattagcgac gttggtcccg ccgcttgaga atctggatga aaataagctc
    17281 cctggacttg gcttattcag agaactggtc aacacttgtc tctcccagcc aggtctgacc
    17341 accgggcaac ttttagagca ctatcgtggt acaaataatg ctgccaccct tgaaaaactg
    17401 tcgatgtggg acgatatagc agataagaat attgctgagc aaaccttcac cgactcactc
    17461 aaccatatgt ttgattcgct gcttgaactg cgccaggaag agttaatcgc tcgtgagcgc
    17521 acgcatggtt taagcaacga agaacgcctg gagctctgga cattaaacca ggagctggcg
    17581 aaaaagtgat ttaacggctt aagtgccgaa gagcgatcgg gaagcccccg acagccgcac
    17641 tgagaggcag cggcaaatat ataagtacgc cctcgtaatt atcgttggcg gtaaacaacc
    17701 gttggatttc agcgttaacg gctgaaggac atcgggtcaa tcgcccaaca ccaacctcat
    17761 gaaataagtg tggataccgt cttatggagc aaaacccgca gtcacagctg aaacttcttg
    17821 tcacccgtgg taaggagcaa ggctatctga cctatgccga ggtcaatgac catctgccgg
    17881 aagatatcgt cgattcagat cagatcgaag acatcatcca aatgatcaac gacatgggca
    17941 ttcaggtgat ggaagaagca ccggatgccg atgatctgat gctggctgaa aacaccgcgg
    18001 acgaagatgc tgccgaagcc gccgcgcagg tgctttccag cgtggaatct gaaatcgggc
    18061 gcacgactga cccggtacgc atgtacatgc gtgaaatggg caccgttgaa ctgttgaccc
    18121 gcgaaggcga aattgacatc gctaagcgta ttgaagacgg gatcaaccag gttcaatgct
    18181 ccgttgctga atatccggaa gcgatcacct atctgctgga acagtacgat cgtgttgaag
    18241 cagaagaagc gcgtctgtcc gatctgatca ccggctttgt tgacccgaac gcagaagaag
    18301 atctggcacc taccgccact cacgtcggtt ctgagctttc ccaggaagat ctggacgatg
    18361 acgaagatga agacgaagaa gatggcgatg acgacagcgc cgatgatgac aacagcatcg
    18421 acccggaact ggctcgcgaa aaatttgcgg aactacgcgc tcagtacgtt gtaacgcgtg
    18481 acaccatcaa agcgaaaggt cgcagtcacg ctgccgctca ggaagagatc ctgaaactgt
    18541 ctgaagtatt caaacagttc cgcctggtgc cgaagcagtt tgactacctg gtcaacagca
    18601 tgcgcgtcat gatggaccgc gttcgtacgc aagaacgtct gatcatgaag ctctgcgttg
    18661 agcagtgcaa aatgccgaag aaaaacttca ttaccctgtt taccggcaac gaaaccagcg
    18721 atacctggtt caacgcggca attgcgatga acaagccgtg gtcggaaaaa ctgcacgatg
    18781 tctctgaaga agtgcaccgc gccctgcaaa aactgcagca gattgaagaa gaaaccggcc
    18841 tgaccatcga gcaggttaaa gatatcaacc gtcgtatgtc catcggtgaa gcgaaagccc
    18901 gccgtgcgaa gaaagagatg gttgaagcga acttacgtct ggttatttct atcgccaaga
    18961 aatacaccaa ccgtggcttg cagttccttg acctgattca ggaaggtaac atcggcctga
    19021 tgaaagcggt tgataaattc gaataccgcc gtggttacaa gttctccacc tacgcaacct
    19081 ggtggatccg tcaggcgatc acccgttcta tcgcggatca ggcgcgcacc atccgtattc
    19141 cggtgcatat gattgagact atcaacaaac tcaaccgtat ttctcgccag atgctgcaag
    19201 agatgggccg tgaaccgacg ccggaagaac tggctgaacg tatgctgatg ccggaagaca
    19261 agatccgcaa agtgctgaag atcgccaaag agccaatctc catggaaacg ccgatcggtg
    19321 atgatgaag
//
LOCUS       DAJTYH010000078        19283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTYH010000078 DAJTYH010000000
VERSION     DAJTYH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            208..1305
                     /gene="mltA"
                     /locus_tag="OO609_002745"
     CDS             208..1305
                     /gene="mltA"
                     /locus_tag="OO609_002745"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6634103.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1543..2349
                     /gene="tcdA"
                     /locus_tag="OO609_002746"
     CDS             1543..2349
                     /gene="tcdA"
                     /locus_tag="OO609_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6634104.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2400..2843)
                     /gene="csdE"
                     /locus_tag="OO609_002747"
     CDS             complement(2400..2843)
                     /gene="csdE"
                     /locus_tag="OO609_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6634105.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2843..4048)
                     /gene="csdA"
                     /locus_tag="OO609_002748"
     CDS             complement(2843..4048)
                     /gene="csdA"
                     /locus_tag="OO609_002748"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6634106.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4240..4467
                     /locus_tag="OO609_002749"
     CDS             4240..4467
                     /locus_tag="OO609_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6634107.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4818..5735
                     /gene="gcvA"
                     /locus_tag="OO609_002750"
     CDS             4818..5735
                     /gene="gcvA"
                     /locus_tag="OO609_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6634108.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5754..6149
                     /locus_tag="OO609_002751"
     CDS             5754..6149
                     /locus_tag="OO609_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6634109.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6142..7242
                     /gene="rlmM"
                     /locus_tag="OO609_002752"
     CDS             6142..7242
                     /gene="rlmM"
                     /locus_tag="OO609_002752"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6634110.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7286..8017)
                     /gene="fucR"
                     /locus_tag="OO609_002753"
     CDS             complement(7286..8017)
                     /gene="fucR"
                     /locus_tag="OO609_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6634111.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8075..8497)
                     /gene="fucU"
                     /locus_tag="OO609_002754"
     CDS             complement(8075..8497)
                     /gene="fucU"
                     /locus_tag="OO609_002754"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6634112.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8499..9918)
                     /gene="fucK"
                     /locus_tag="OO609_002755"
                     /pseudo
     CDS             complement(8499..9918)
                     /gene="fucK"
                     /locus_tag="OO609_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10027..11802)
                     /gene="fucI"
                     /locus_tag="OO609_002756"
     CDS             complement(10027..11802)
                     /gene="fucI"
                     /locus_tag="OO609_002756"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6634113.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11835..13151)
                     /gene="fucP"
                     /locus_tag="OO609_002757"
     CDS             complement(11835..13151)
                     /gene="fucP"
                     /locus_tag="OO609_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6634114.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13698..14345
                     /gene="fucA"
                     /locus_tag="OO609_002758"
     CDS             13698..14345
                     /gene="fucA"
                     /locus_tag="OO609_002758"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6634115.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14373..15521
                     /gene="fucO"
                     /locus_tag="OO609_002759"
     CDS             14373..15521
                     /gene="fucO"
                     /locus_tag="OO609_002759"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6634116.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15574..16329)
                     /gene="xni"
                     /locus_tag="OO609_002760"
     CDS             complement(15574..16329)
                     /gene="xni"
                     /locus_tag="OO609_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6634117.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16440..17807)
                     /gene="sdaB"
                     /locus_tag="OO609_002761"
     CDS             complement(16440..17807)
                     /gene="sdaB"
                     /locus_tag="OO609_002761"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6634118.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17865..19154)
                     /gene="sdaC"
                     /locus_tag="OO609_002762"
     CDS             complement(17865..19154)
                     /gene="sdaC"
                     /locus_tag="OO609_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6634119.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4713 a         4757 c         5116 g         4697 t
ORIGIN      
        1 ccgtggatgc gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca
       61 cggatttagc atgattttga acaaaaacaa accactaaag cctatttttt gtgcaaaatt
      121 tgcgtcaggg agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc
      181 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      241 ggcacggttg tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa
      301 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      361 gcgccgatta acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      421 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      481 ggtgataccc gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgccgac
      541 aactatggta acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      601 cgccagggcg agttccagta tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg
      661 tctcgtgcgg agatctacgc cggggcattg ggcgataaat acatcctcgc ttacagcaac
      721 tctctgatgg ataacttcat tatggatgtg cagggtagtg ggtatatcga ctttggtgat
      781 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      841 ggtaaggtgc tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      901 cgtcattggg gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg
      961 tctttcgtct tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg
     1021 ctggttggcc gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg
     1081 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1141 ctgatggtgg cgttggatgt cgggggcgca atcaaaggcc aacacttcga tatctatcaa
     1201 gggatcgggc cggaagccgg acaccgcgca ggttggtaca accactatgg acgggtttgg
     1261 gtgctgaaaa cggctccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat
     1321 gccgttttta cggatatgac aggggagggg taacctcaca tcctgattat tgactaagtg
     1381 cgcgttgttc ataccggatg cggcgtgaac gccttatccg gcctacaaat catgcaaatc
     1441 caaaatattg caggagttat gtaggcctga taagcgtagc gcatcaggca tttatgcgtt
     1501 tagggtgagg gataacctcg ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt
     1561 gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt atggtgaaaa agcgttgcaa
     1621 ctgtttgctg acgcgcatat ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg
     1681 gaagcgctgg cgcgcacggg gattggcgca atcacgctta tcgatatgga tgatgtgtgc
     1741 gtcaccaata ccaatcggca aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg
     1801 gaagtcatgg cggagcgcat tcgccagatt aacccggagt gccgtgtaac ggtggtggat
     1861 gatttcgtga cgccggataa cgtagcgcaa tatatgagtg ccggttattc gtacgtgatt
     1921 gatgccattg atagtgtacg gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa
     1981 atcccgctgg tcacgaccgg tggcgcgggt gggcagattg acccgacgca gattcaggtt
     2041 accgatctgg cgaaaacgat tcaggatccg ctggcggcga agttgcgcga gcgcctgaaa
     2101 agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg gcgtggattg cgtgttttct
     2161 actgaagcgc tggtgtaccc gcagtcagac ggtacggtgt gtgcgatgaa agccacggca
     2221 gaagggccga agcggatgga ttgtgcatct ggatttggcg cggcaacgat ggtgaccgcc
     2281 acctttgggt ttgttgcggt ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt
     2341 cagggttaaa caatcaggtc ggatgagacg cgaacgtctt atccgacccg gttctgtgct
     2401 taaacctgct tcgtcgcagc gataatcgcc tcgcttaacg catttaaccc ctggctgcgt
     2461 gaggcgctaa gctgcgcacg taatcccagc tcatcaaaca atgccagcgg cgactgtgcc
     2521 tgtaattcgg cggcggtttt cccctcaacg gcagtcaaca acaccgccaa caggccgcgc
     2581 acaatgcgcc cttcgctgtt gccaaagaag tgcattttgc cgttttcagc cacggtatat
     2641 cccagccaga cgcggttttc gcatccggca atctctttag cctgcgcttt taactcgtct
     2701 ggcaaagccg gaagctgttt ccccagcatg atcaactggc gatatttatc ttcccattgc
     2761 gacaacggtg cgaaggtatt gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt
     2821 ccggcgaatt gcgggtttgt cattaatcca ccaataattc cagcgcgcgg tcaacggcat
     2881 tcaccagcgc atccacatca ctctttgtat tatatggcgc aaatgaggcg cgcagtgtgc
     2941 cggttacgcc taattctgcc agtagcggct gagcgcaatg ttgcccggcc cgcaaggcaa
     3001 taccgtactc cgccagtagc gtcaccatat cgctgtgatg aacgccagca aaatcaaagg
     3061 ccagcaggct ggaatcctgg cagcggaatg aacgaaagcc tggacgtttc gccagcgctt
     3121 cttctgcaag cgttgctaag ctacggctcc agctttcggc ctggttgata tcgtaatctg
     3181 ccagccattc cagcgccgcg cttaatccta tgacgccagc gacatttggc gttcctgctt
     3241 ccagtttcca cggcgcagat tgagtggtga aaccgtcaaa actcacttcg tgaatcattt
     3301 tgccgccacc cagccagggc gacatcgctt ccagcagttc tgatttgcca tacagcacgc
     3361 cgatacccgt cgggccatac agtttgtgac cagagaaagc atagaaatcg atatccagtt
     3421 gctgaacatc cgcggggaaa tgcaccgtcc cctgagcacc atcaaccatc accaccatcc
     3481 cggctgaatg agcaaaggta atcgctcgcg ccagatccgg gcaaccgcca gtcacgttcg
     3541 acatctgacc caacgccaga atccgactac ggggagtaat cagctctggc aacaaatcga
     3601 catccggcag tcgctgcgca ttaagcggca atttcaccac tttggctcta gtttgttggg
     3661 cgaccatcag ccaggggacg aggttagcgt ggtgttctgc cacgctgaca ataatctcat
     3721 cgcccggttg cagacgcgga cgcgcatagc attgtgccac catgttgatg gattcagtgg
     3781 tgccgcgcgt ccagacgata gttttatcat ccggtgcatt cagtaattgc gccactttct
     3841 ctcgtgcagc ttcataacgc gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga
     3901 cgtttccggc gctcaggctg taaaactgtc gagtggcttc aaccacggct tcaggtttaa
     3961 gcgcggtcgc ggcgctgtcg agatagacgc ccgcatcctg tagtgcggga aactgggcgc
     4021 gaaactgcgc gggattaaaa acgttcatgg tactcctcgg cttgatgcac cgatcgtgac
     4081 gcaaaattca ctttgataca aggagagtgc taaccatcag aattatctgg atatcctggc
     4141 gaagccgcgt aagtaacata tgctgaatag tgttaggcgt taattttagc ctgttaatca
     4201 atactattaa gatttattaa tcgttttaag gagaagatga tgaaaaagac tgccgcaatt
     4261 atttctgcct gtatgctgac ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg
     4321 cacaccaatg acggacgtac catcgtctct gacggcaaac cacagactga taacgatatc
     4381 ggtatgattt cgtataaaga cgctaatggc aacaaacagc agatcaaccg tactgacgtg
     4441 aaagagatgg tcgaactgga acagtaattc gcgatcgcaa ggtaaaaaaa agcaccgcaa
     4501 ttaggcggtg ctacattaat cactatggac agacagggta aatgtacagg aagtgaaaaa
     4561 aggtagcttt gctaccatgg tctgaatcgc agaccaattg caaacacaac aacacaacat
     4621 cacaaccgta agccaaaagt tcaccagaat acgcattccg ataaaacttt tcgttccggc
     4681 tcaggaagtg ccgccactat aggtatttgc tggtagaagc tcaacggaca atttataatg
     4741 gctcagatta aaaaaactaa taggttacac agtgtgatct aattgttaaa ttcatttaac
     4801 atcaaagttt aaaagccatg tctaaacgat taccaccgct aaatgcctta cgagtttttg
     4861 atgccgcagc acgccattta agttttactc gcgcagcaga agagcttttt gtgacccagg
     4921 ccgcagtaag tcatcaaatc aagtctcttg aggatttttt ggggctaaaa ctgttccgcc
     4981 gccgtaatcg ttcactcctg ctgaccgagg aagggcaaag ctatttcctc gatatcaaag
     5041 agatattttc gcaattaacc gaagcgacgc gtaaactcca ggcccgtagc gccaaggggg
     5101 cgttgacggt cagtttactc cccagtttcg ccattcattg gttggttccg cgactttcca
     5161 gctttaattc agcttatccg ggaattgacg ttcgaatcca ggcggttgat cgtcaggaag
     5221 ataagctggc ggatgatgtt gatgtggcga tattttatgg tcggggcaac tggccggggc
     5281 tacgggtgga aaaactgtac gccgaatatt tattgccggt gtgttcgccg ctactgctga
     5341 caggcgaaaa acccttgaag accccggaag atctggctaa acatacgtta ttacatgatg
     5401 cgtcacgccg tgactggcag acatataccc gacagttggg gtttaatcat atcaacgttc
     5461 agcaagggcc aatttttagt catagcgcca tggtgctgca agcggctatt cacgggcagg
     5521 gagtggcgct ggcaaataac gtgatggcgc aatctgaaat cgaggccgga cgtcttgttt
     5581 gcccgtttaa tgatgttctg gtcagtaaaa atgcttttta tctggtttgt catgacagcc
     5641 aggcagaact gggtaaaata gccgcctttc gccaatggat cctggcgaaa gccgctgctg
     5701 aacaagaaaa attccgcttt cgttatgaac aataatttac gtagggtacg accatgacca
     5761 gccgttttat gctgattttc gccgccatta gcggcttcat ttttgtggca ctgggcgctt
     5821 ttggtgcgca tgtgttaagt aaaaccatgg gggccgttga gatgggctgg atccaaaccg
     5881 gtctcgaata ccaggcgttt catacgctgg cgatcttagg tctggcggtg gcaatgcagc
     5941 gtcgcatcag tatctggttt tactggagta gcgttttcct cgcgttaggc acggtgctgt
     6001 tcagcggcag cctttattgc ctggcgctgt cccatctgcg tttgtgggcg tttgtcacac
     6061 cggttggcgg cgtgagcttc cttgcgggct gggcgttaat gttagttggt gctatccgtt
     6121 taaagcgcaa gggcgtaagt catgaataag gttgtattgc tgtgccgtcc gggctttgaa
     6181 aaagagtgcg ccgcagaaat taccgataaa gccggccagc gggaaatttt cggttttgcc
     6241 cgcgtgaaag agaatgcggg ttatgtcatt tatgaatgtt atcaacctga tgatggcgat
     6301 aagttaatcc gtgagctgcc gttcagttca ttaatttttg cccgccagtg gtttgtggtg
     6361 ggggaactcc tgcagcattt gccgccagaa gatcgtatta cccccattgt cggcatgtta
     6421 cagggtgtag tagagaaggg cggtgaactg cgtgttgaag ttgccgatac caacgaaagc
     6481 aaagagttgc tgaaattctg ccgtaagttc actgttccgc tacgcgctgc cttgcgcgat
     6541 gctggggtgc tggcgaacta tgaaacgccg aagcgtccgg ttgtgcatgt attctttatt
     6601 gcaccaggat gctgctatac cggttactca tacagcaaca ataattcgcc gttctatatg
     6661 ggcattccgc gcctgaaatt tccggcagag gcgccgagtc gttccacgct caaactggaa
     6721 gaggcatttc atgtgtttat tcctgcggat gagtgggatg aacgcctggc gaacgggatg
     6781 tgggcggtgg atttaggcgc ttgccctggc ggctggacct accaactggt gaagcgcaac
     6841 atgtgggttt attccgtcga caacggcccg atggcgcaaa gtctgatgga taccgggcag
     6901 gtgacgtggc tgcgggaaga cggtttcaaa ttccgtccga cacgcagcaa tatctcctgg
     6961 atggtatgcg atatggttga aaaaccggct aaagttgcgg cattgatggc gcagtggctg
     7021 gttaatggct ggtgccgtga aaccattttc aatctcaaac tgccgatgaa aaaacgctac
     7081 gaagaagtat cacacaatct ggcgtatatt caggcacagc ttgatgaaca tggcataaat
     7141 gctcagattc aggcgcggca gttgtatcac gatcgcgaag aagtgacggt gcacgtccgc
     7201 cgcatctggg ctgcggtggg tggtcgtcgc gacgagcgat aacaggcaag gccggggcgt
     7261 cgtctccggc ctgctaccct ttccgtcagg ctgttaccaa agaagttgca acctggcgct
     7321 catcagaaat aatgtgcgtt acctcatcca gatgcccgat gcgggcttcc cctgaacgat
     7381 taaatttact tttatcaatc aataacaacg attgcgcggc acgttttaat agcatcgatt
     7441 tgtaatcagc gttgatcgca ttggagtccc acagtgcgcc gctgctatcg atcccttcac
     7501 aagaaaaaat aaacagatcg atttccagcg atttaagttg ggaaatcagc gagggattga
     7561 cgtaacagcc atatttgcgc tcaagtgtgc cgccggaact gatcagttga atgcgttcgc
     7621 gtttaccgag ttcatggcaa atcggatggc tattggtgaa gacctgaatg ttgatgtcag
     7681 gcaactggcg tgccagatac cagcaagttg aactggcgtc taaggctatc accatccctt
     7741 cttcaatcca cgcgagcgcc tcgcgcgcga tatctgcttt atgcgcataa tggcttttca
     7801 gcctgatgtg aaagggatcg ccactgtctt gattttgacg gtggatatat ttagcgcgtc
     7861 catgattgcg cagaatttta ccctgcgtct gtaattcatt gagatcgcga cgaatggttt
     7921 ctttactgac ctttagctgt tcagagagag cttccgtggt caggctggta tggttcagca
     7981 gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt tttcacccca tcattcgata
     8041 accgcccgcg tcatgcgggc gtaccggtat gagattacgg tgttacccct ttttttaaaa
     8101 gaatattccc gtacttcgct cgttcgcctg tgataacgat cgcaaaggct ttttgcgccc
     8161 gttcataaaa cgcaaaacga ttgatgcgga tgatgtcagg acacggggct tgtagtgaaa
     8221 gcgcattacg gtaacgtcgt tctacttcag gatcgagagt gtcaccctct accgccgcca
     8281 tcatcaccag cggcggggca taactgtcca gctcaaataa cgggataatc gcctggagca
     8341 agtcgctcac caacaggcca tcagcgcgga tcacctgcgg ttccatcgaa tgggcgggaa
     8401 agtgagcatc ggaaaaaata atttcatctc catgtcccat ctctgccagc actttcaata
     8461 gttcgggaga aattaacggc gaaattgttt tcagcatttc acacttcctc tataaattca
     8521 ggttcagttt gcgggtagaa ataacggtac tgataatgaa tctgtgcacg ggcttcttcc
     8581 gggctgttaa attcccctac gccataccaa ccgaacagcg cagctcctgc gacggtcgtt
     8641 tcggcatcgt cgagaacttt taccggaata tcaagcatat tggctttaat ctgattccac
     8701 aatgtgttgc gacttcctcc accgactaac aataattcat aggctttaaa gtgcccgatt
     8761 ttttccagca tctgtagatt gcgctgtaat tgcgcagtta acccttccag cgccgcgcga
     8821 tagaaatgcc cccgcgtggt attaagcgtc actccttgcc agccagcgtt ctgacacgac
     8881 aataaatcac actgcatttt cacaccatcc gcgccaggtg cgatcagacg agcttcttca
     8941 atcaacattt gccagggtgt ttcagccgtc cagaacagtt ttctcaccca ttccagcacg
     9001 ccggatgcca gccattgcat acctgggtta tacaaccatg cctggctatc tagttcgcag
     9061 gtggaaccgg cgtactgact taacagcgaa gtatcaacct aggcgctgcg aaccattaaa
     9121 atttcccatg taccggagga gagcacgggt tcattttgtt cggcaccagc gccaaaaagg
     9181 gcgaactggg tatcgtgacc tgcggaaatc accggtatgc caacgggtaa gccgagcatt
     9241 gctgcggcgt tgttctgtag cgtaccaatc tgttcacccg cttccaccag acgagggaag
     9301 agtcggcgtg gaataccggt ggcttgtaaa atttgcggac tgaaatcgcg ttgctggata
     9361 tccagcatct ggctggttcc ggccatcgtg atatcagtag tgaattcgct ggttaaacgg
     9421 tggttaatca gcgacgaaat gaagagccag gcgtgcgcgc gttccagcag ttgtggatga
     9481 ttttctttca accacaccaa cttatataac gtattgaaac taaaggctcc gacgccagaa
     9541 atagcctgca accgctgtgc ggagattaac cgttcaatat tgtccataac cgctgctgtt
     9601 cgcggacatt tccagctaat gatcggatag agcagattgc cttgcttatc taccagagcg
     9661 ccatccacac taaaggtggt gacggcgata ccgcggatgt ggcattcagt cagttcacta
     9721 ttgatttgcc gacagcaatc agcaaagcgt tgcaaaatgg cagtctaaag accactggtg
     9781 ccaggtgttg ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat
     9841 ttttccctgc cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag
     9901 gataacttct tgtttcataa tcgctcctgc aatatagccg gataacattg tttatccggc
     9961 taaccactct tgcttgttca ggcccggtag ccggagcgac cgggcatcac atcagggtgt
    10021 aatgtattaa cgcttgtaca acggaccgta gttctggcaa gcgcggtaat cctggccttc
    10081 aatatccatg ccgtgcgcag cccaggcaga aggacgatac actttggtct cttcaacgtt
    10141 gtgcatacat accgggatac gcagcatgga ggcaagggtg ataaagtctg cgccaacgtg
    10201 gccgatggtc agaactccgt ggttggcacc ccagttcgcc attaccgaat acacatccgt
    10261 aaacggccct ttaccagtga ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt
    10321 gcgtttgttg aggatgtcat gcacatcctt cggcaattcc acgctccagc cttccgcgat
    10381 ttgcagtacc ggtcccaggc ctttgatgat gttgacacga gtcatggtga acgggacgcc
    10441 gccttcggta aggaagcggg aagagtaacc gccgccacgg aagtattcgt ggatcgccgg
    10501 gcaccattcg gtagcggcga ggcaagcgtc agcctcttgc tgagagattt cccagtgcgg
    10561 cttcatcgtc gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga
    10621 acctgagttg atcaaatgga tgatgccgtg ttctgccagt ccgtccagtt tatgccccgt
    10681 tacacgctca attgcttctg gtgaccagta ggtacgcaca tcggcaaata cctgagcggt
    10741 gccggtgagc tggtgaccca ttagcattgc cacgccgtta agactgtcgt tttcggtcgc
    10801 cacgacaaag ggttcgcgca cgccattcca gtcaaatgaa ctgttgagga tcgcttcggc
    10861 ggtgtcacca ttgggatatt gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat
    10921 ggcgttgtag ccaagtgatt cttccacgcg accaatatcg gccagtttgc tgttgccttg
    10981 catcatgtcg cggatacaca tcgccatcag taaactttcg cgcagaactg cgcggctttg
    11041 ctcggcattg cgttgatact gtttgttatt ttcatcttcg ccatagcgga agtttttatc
    11101 agcccaggcc agtgccattt ccagttcggc ttcgtcgtaa atcttctgat cgatacggcg
    11161 acgcagttcg gtcatatcca ccgcctggac tttcattccc agccaggatt caaagaagtt
    11221 gtgatcaaca atagaaccgg cgatacccat cgaaacgccg cccagcgaca gatagctttt
    11281 acctttcatg ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac
    11341 atcggcagga atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc
    11401 cgggatgcct ttctggctgt gagctgccag agccgcagcc aggtaaacag cgccggggcg
    11461 ttcagtgccg ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt cgatggtttc
    11521 actgccatag caccagcaag gcgttaccgt aatggtgagg cctacattct ggctgctgaa
    11581 tttttcttcg caagcagcgg cttcagccat acccgcgata caggtatcgg aaatgacaca
    11641 ctcgacggca gctccgcagg catggcgcag tttctcggtc agcagtgcgg ccgtagcttt
    11701 cgccatattc attgtttgtt cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat
    11761 aaccgggcgg ataccaattt tcggtaagct gatttttttc attacagatt cctcacttta
    11821 ttcggaaaat atgttcagtt agttgccgtt tgagaacgga aacgggcaaa gataaagatg
    11881 accgcgaagc agagtgcggg gatcagttca gcagtgggga tgttgcccgc cgcgtcactg
    11941 acaaaaccca tgaccggagt gacaataccg ccgccaataa tggtcataac gatgaaggac
    12001 gaaccgtatt tggtgtcctg gccgagattc ttaatgccca gcgagaagat tgttgggtac
    12061 tgaatcgaca taaaggcgct gcataaagtc agggctatta agcccacatg accgccagcg
    12121 aaggctgaga tcaggcacag tgccatagcg attaatgcgt aggcggccag gactttgtgt
    12181 ggtgcgaagc gactgatgag ccaggtaccg gtgaaacgac caataaagaa gcacaccatg
    12241 gttccggtta aatagttagc ggcaaagcct gcagtcatac ctagaatttc ttctacagcg
    12301 tagcgaatca aatagctcca gcaggccgtt tgtgcgccga catagcagaa ttgcgctaat
    12361 accgcccagc gccagtgacg aatacgcgcc agacgagaaa gcgatgcgga gaacgatcct
    12421 tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc
    12481 agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc
    12541 aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat
    12601 tgatgtggca cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca
    12661 aacgagttaa atgtttgcgc aagatttaag cggaagtgac tactgctttc cggccctaat
    12721 accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc aataataaat
    12781 aggccaacta aaaataaggt gtagttcatt atttctgcag cgggccagaa taatgcagcg
    12841 cccaaggcat ataaaaataa tccggtaata atccctgctt tataactgag ttttttcatc
    12901 aatatcccag cagggattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg
    12961 ccagcctgga aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta
    13021 aggttattgg ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg
    13081 taacttctgc tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat
    13141 gtgtttccca tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc
    13201 aaaagtgcaa aaccagcgag ttcgggcttc atatcggtga atatttgcct gtctgaaatg
    13261 aactcatgga ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat
    13321 tagctggaag gtgactttat gtgactacca tcactttaat aatcgaaatt tcatgcccgt
    13381 ttcagaaaat aaatcataaa aaacacaaac cagatgaccg tattaattgt ttgtgccgaa
    13441 aacgggcatt tctggcgagg ggaaatggaa aaatgaccgt tttgataaat atttttaaga
    13501 gcggtcattt ttaacggggt tataaaaaga gaacttgcag ttatttactg tgattagttg
    13561 agctaggtca caaaatataa gcttgtgaat ttctttcttg ttaatcagga agaagatggt
    13621 gggtaattaa acggctaatt caatagtgtg aaaggaacaa cattattgcc ctgttttgaa
    13681 tcagagagag gtagctaatg gaacgaaata aacttgctcg tcagattatt gacacttgcc
    13741 tggaaatgac ccgcctggga ctgaaccagg ggacagcggg gaacgtcagt gtacgttatc
    13801 aggatgggat gctgattacg cctacaggca ttccatatga aaaactgacg gagtcgcata
    13861 ttgtctttat tgatggcaac ggtaaacatg aggaaggaaa gctcccctca agcgaatggc
    13921 gtttccatat ggcagcctat caaagcagac cggatgccaa cgcggttgtt cacaatcatg
    13981 ccgttcattg cacggcagtt tccattctta accgatcgat ccccgctatt cactacatga
    14041 ttgcggcggc tggcggtaat tctattcctt gcgcgcctta tgcgaccttt ggaacacgcg
    14101 aactttctga acatgttgcg ctggctctca aaaatcgtaa ggcaactttg ttacaacatc
    14161 atgggcttat cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg catgaagttg
    14221 aagtgctggc gcaactttac ctgacgaccc tggcgattac ggacccggtg ccagtgctga
    14281 gcgatgaaga gattgccgta gtgctggaga aattcaaaac ctatgggtta cggattgaag
    14341 agtaatttcg taaagcaaca aggagaagga tgatggctaa cagaatgatt ctgaacgaaa
    14401 cggcatggtt tggtcggggt gctgttgggg ctttaaccga tgaggtgaaa cgccgtggtt
    14461 atcagaaggc gctgatcgtc accgataaaa cgctggtgca atgcggcgtg gtggcgaaag
    14521 tgaccgataa gatggatgct gcagggctgg catgggcgat ttacgacggc gtagtgccca
    14581 acccaacaat tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc ggcgcggatt
    14641 acctgatcgc tattggtggt ggttctccac aggatacttg taaagcaatt ggcattatca
    14701 gcaacaaccc ggagtttgcc gatgtgcgta gcctggaagg gctttccccg accaataaac
    14761 ccagtgtacc gattctggca atccccacca cagcaggtac tgcggcagaa gtgaccatta
    14821 actacgtgat cactgacgaa gaaaaacggc gcaagtttgt ttgcgttgat ccgcatgata
    14881 tcccgcaggt ggcgtttatt gatgctgaca tgatggatgg tatgcctccg gcgctgaaag
    14941 ccgcgacggg tgttgatgcg ctcactcatg ctattgaggg gtatattacc cgtggcgcgt
    15001 gggcactaac cgatgcactg cacattaaag cgattgaaat cattgctggg gcgctgcgag
    15061 gatcggttgc tggtgataag gatgccggag aagaaatggc gctcgggcag tatgttgcgg
    15121 gtatgggctt ctcgaatgtt gggttagggt tggtgcatgg tatggcgcat ccactgggcg
    15181 cgttttataa cactccacac ggtgttgcga acgccatcct gttaccgcat gtcatgcgct
    15241 ataacgctga ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg ggcgtgaaag
    15301 tggaaggtat gagcctggaa ggggcgcgta atgccgctgt tgaagcggtg tttgccctca
    15361 accgtgatgt cggtattccg ccacatttgc gtgatgttgg tgtacgcaag gaagacattt
    15421 cggcactggc tcaggcggca ctggatgatg tttgtaccgg tggcaacccg cgtgaagcaa
    15481 cgcttgagga tattgtagag ctttaccata ccgcctggta aatgcgctga tgtgataatg
    15541 ccggatacga tttgcggcgt atccggctcg ccgttaccgt accaaccgca attgctgtaa
    15601 attgccgtcg atatgcaaat cggtttgtaa gcgggcaata tcgcggcaca gaaacgccat
    15661 ctctttatgg gtttctaatt ttttgcgcca cttttcggca accgcatcca gattctcata
    15721 tatcccttcc agactctgaa actcgaccag cagctgcgtg gcgctttttg gtccgattcc
    15781 cgcaacaccc ggtacctttg aactgctgat ccccgcaagt ccccagtaat cgggcaactg
    15841 ctgcggttga acgccaaatt ctttatcgat aaatggcgca tccagccaac gtttctggaa
    15901 gtaatcacga atacgtaatg tcggtgaaag taactgacag tagcctttat ctgtcgaaac
    15961 aatcgttgcc tgatgcccgg cctgtgtcac tttgaccgcc agtgtggcgg ctaagtcatc
    16021 ggcttcgttg ccgctggctg accagcacgg gacgccgcgt tgctcaaagg cggcgcgtaa
    16081 tgcaggcatc tcgtcgtgca actcttccgg cattggcggt cgatccgctt tgtaatctgg
    16141 taaacgctga tgacgccagc cgctactgcg gttttcatca tcaaaaacgg cgaccgcgtg
    16201 ggttggctgg ctgtgcataa tgagctgatc gagcgcatgc tggcaggttt cgacacaggg
    16261 cgaaccctga acggcatgaa tgcgacggat aagattcagt gcatcgacaa taagcaaatg
    16321 aacagccacg ataaccccct taaagatcag gcgataaggg taacattcga tgccggaaca
    16381 ggctatggct ggttgagaaa acaggaagag gcttcgcaaa acgaggcctc tgggagagat
    16441 taatcgcagg caacgatctt catcgccagg ccgccgcgag aggtttcgcg gtacttggcg
    16501 ttcatgtctt tacctgtttc gtacatggtt tcgataactt tatcgaggca gacgcgcggt
    16561 tcgctggtac ggcgcagtgc catacgcgcg gcgttcaccg ctttcactgc tgcaatggcg
    16621 ttacgctcaa tgcacggtac ctgaacctgc cccgcgaccg ggtcacaagt cagaccgagg
    16681 ttgtgctcca tcgcgatttc cgctgcgatg caaacctgcg ccgggcttgc gcctaacagt
    16741 tctgccagac cagctgccgc catagagcac gccacgccaa cttcaccctg gcaacccact
    16801 tcggcaccag aaatcgacgc gttcatctta taaagagaac caatggcgct ggctaccagc
    16861 aggtaacgag ccagtgagtt agcgttcact tcgcggataa acttatcgta gtacgccagc
    16921 acagccggga taatcccgca cgcaccgtta gtcggcgcag tcaccacgcg accgccagca
    16981 gcgttctctt cgttcactgc cagtgcaaac atgttgatcc agtcaacaac cgccatcggg
    17041 tcagtggtgg ttttatcctg gctgaccagc atccggcgta gtgccgcagc acggcgtgga
    17101 acgcgcagtt tgccaggcaa cacgccttcg gtggaaatac cgcgctcaat accgccacgc
    17161 atgacttccc agacgttcgc caggtgctgt tccagctctt ctttactgtg cagcgccagc
    17221 tcgtttttca tcatcaggcc agagagtgac agcccggttt cctgacaatg tttttgcaga
    17281 tcggctgctg aactgtacgg ataaggaact tcaaccggtg cgctatcctg ctggccaaaa
    17341 tgctcttcat caacgataaa gccaccgcca atagagtagt aagtctggct gtaaacgact
    17401 ttatcgcccg ccagcgcggt aatgcgcata ccgttttcat gcagagaaag gttgtctgca
    17461 tggaagttca tgcactgatc aaccgggaac tccacttcat gctgaccgtt tgccagcatc
    17521 aggcgaccat gagtattcac atcctgaata aaaccgggga tggaatcgat atccacggta
    17581 tccggcaggt tacccgccag gcccataata atggcgatat ctgtgtggtg gcctttacca
    17641 gtcagagaga gcgagccgta cacgtcaacc accacgcggg tcacgtcttt aagcaggtta
    17701 cgggcaatca gatcgtcggt aaattgttta cccgctttca ttggtccaac ggtatgagaa
    17761 ctggaagggc caatgccgat tttgaaaata tcgaatacgc taatcatagg aaatacatcg
    17821 cgttaaaacg gaggaagcgc cgcccgaaag cggcgcgaaa ggacttagct gaacagagag
    17881 tagaagattg cggagattgc aatcagaccc atcacgacaa cgaatacgtt gctgatgtga
    17941 ccgctgtact tacgcattgc cggtactttc tgaattgcgt acatcggcat caggaacagg
    18001 atcatcgcga tgattgggcc gcccagggtt tcaatcatac ccaggatgct cgggttcagg
    18061 gtggcaacaa tccaggtcgt taccagcatg aacagcgcag tgatacggtt cagcttgttg
    18121 atttcgatag acttaccttt accacgcaga gatttaatca ccataccgtt gaagccttca
    18181 cgtgcgccca ggtagtggcc gaggaaggat ttggtgatag cgataatcgc gataatcgga
    18241 gccatccacg cgataaccgg tgcgttaaag tggttagcca ggtaagacag aatcgagatg
    18301 ttctgctctt tagccgcagc caggtctgcc ggagtcaggc tcagtacgca gctgaagacg
    18361 aagaacatta cggtcagcac catcatgatg tgtgcgaatg ccaggatctt cgagcatttc
    18421 tgttctgcca tatcgccgta ctcttcacgc ttcgcaacgg cgaaagaaga gatgatcgga
    18481 gagtggttga acgagaacac cattaccgga attgccagcc acagggtcat ccacagaccg
    18541 tttccggttg cagatgcagt gtccagagac agcgtttcca gtgctgcgcc gttccactgc
    18601 gggatcaggt acagagccag cagcatcagt acgccaacaa acgggaatac cagaatactc
    18661 atcgctttaa cgatcatctg ctcaccaaag cgaacgatgg tcatcatacc cacgatcagg
    18721 atcagcgaca gaatcgcacg cggcggtggc gtcataccca gctggtgaga catgaagctt
    18781 tcaacggtat tggtgattgc cacgctataa accagcagga tcgggtagat agcgaagaag
    18841 tagagcaggg taatcagttt acctgcgcca ataccaaagt gttcttctac aacctcggtg
    18901 atgtcttcgc ccgggttttt accagacagt acgaagcgag tcaggccgcg gtgagcaaaa
    18961 aacgtcatcg ggaacgcaag gatagccatg atgatcagcg ggatcatacc gccaacaccg
    19021 gcgttgattg gcaggaacag cacgcccgcg ccgattgccg tgccgtaaag gcccagcatc
    19081 cacatggtgt ctgtcttgcg ccaggcacta cgagagtctt tcgacgcaat cgtgctggtt
    19141 tgagtcgttt ccatctattt ctcctggagg aatgcaaata attcagcttt tagccagttc
    19201 agcccgggaa ggtgcctgac cgtaaaaatg aaatctgtct atcggcgatg gcttaatcat
    19261 ttaccgccgt aataatttga ggg
//
LOCUS       DAJTYH010000079        18789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTYH010000079 DAJTYH010000000
VERSION     DAJTYH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1577
                     /gene="pbpC"
                     /locus_tag="OO609_002763"
     CDS             <1..1577
                     /gene="pbpC"
                     /locus_tag="OO609_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HCR6634120.1"
                     /translation="LAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQN
                     WKGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFV
                     YGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLE
                     AYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKL
                     RLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAW
                     AIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSV
                     TRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKG
                     QRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTG
                     VRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLL
                     VMDDVGQIATVKFVMQ"
     gene            1727..2158
                     /gene="ndk"
                     /locus_tag="OO609_002764"
     CDS             1727..2158
                     /gene="ndk"
                     /locus_tag="OO609_002764"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6634121.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2308..3462
                     /gene="trmG/rlmN"
                     /locus_tag="OO609_002765"
     CDS             2308..3462
                     /gene="trmG/rlmN"
                     /locus_tag="OO609_002765"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6634122.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3747..4760
                     /gene="rodZ"
                     /locus_tag="OO609_002766"
     CDS             3747..4760
                     /gene="rodZ"
                     /locus_tag="OO609_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6634123.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4787..5914
                     /gene="ispG"
                     /locus_tag="OO609_002767"
                     /gene_synonym="gcpE"
     CDS             4787..5914
                     /gene="ispG"
                     /locus_tag="OO609_002767"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6634124.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6017..7291
                     /gene="hisS"
                     /locus_tag="OO609_002768"
     CDS             6017..7291
                     /gene="hisS"
                     /locus_tag="OO609_002768"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6634125.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7309..7929
                     /gene="yfgM"
                     /locus_tag="OO609_002769"
     CDS             7309..7929
                     /gene="yfgM"
                     /locus_tag="OO609_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6634126.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7940..9118
                     /gene="bamB"
                     /locus_tag="OO609_002770"
     CDS             7940..9118
                     /gene="bamB"
                     /locus_tag="OO609_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6634127.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9236..10708
                     /gene="der"
                     /locus_tag="OO609_002771"
     CDS             9236..10708
                     /gene="der"
                     /locus_tag="OO609_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6634128.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10778..10993
                     /locus_tag="OO609_002772"
     CDS             10778..10993
                     /locus_tag="OO609_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6634129.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10990..12360)
                     /gene="xseA"
                     /locus_tag="OO609_002773"
     CDS             complement(10990..12360)
                     /gene="xseA"
                     /locus_tag="OO609_002773"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6634130.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12522..13988
                     /gene="guaB"
                     /locus_tag="OO609_002774"
     CDS             12522..13988
                     /gene="guaB"
                     /locus_tag="OO609_002774"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6634131.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14057..15634
                     /gene="guaA"
                     /locus_tag="OO609_002775"
     CDS             14057..15634
                     /gene="guaA"
                     /locus_tag="OO609_002775"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6634132.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15729..16268)
                     /locus_tag="OO609_002776"
     CDS             complement(15729..16268)
                     /locus_tag="OO609_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6634133.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16284..16802)
                     /locus_tag="OO609_002777"
     CDS             complement(16284..16802)
                     /locus_tag="OO609_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6634134.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17113..17304)
                     /locus_tag="OO609_002778"
     CDS             complement(17113..17304)
                     /locus_tag="OO609_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6634135.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17322..17474
                     /locus_tag="OO609_002779"
     CDS             17322..17474
                     /locus_tag="OO609_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634136.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17656..>18789
                     /gene="pdeF"
                     /locus_tag="OO609_002780"
     CDS             17656..>18789
                     /gene="pdeF"
                     /locus_tag="OO609_002780"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6634137.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4439 a         4571 c         5091 g         4688 t
ORIGIN      
        1 ggctggcacc ccgacaaatg ccgcaactgg caccgctgtt ttcgcgcatg atgctcggta
       61 aaagcaaaag cgacaaaatc gttactacgc tggatgccgg tcttcaacga cgtctggaag
      121 aactggcgca aaactggaaa gggcggttgc caccgcgcag ctcactggcg atgatcgtgg
      181 ttaatcatac cgatatgcgt gttcgcggct gggtgggatc ggttgatctc aacgatgatt
      241 cacgctttgg tcatgttgat atggtcaatg cgatccgatc gccaggatcg gtgctcaaac
      301 cgtttgttta tggtctggcg ctggatgaag gcttgatcca cccggcatca ctgctgcaag
      361 acgtcccccg gcgcaccggt gattatcgac caggtaactt tgatagcggt tttcatggcc
      421 cgatcaacat gagcgaggcg ctggtgcgct cgctgaactt acctgctgtg caggtgctgg
      481 aagcctatgg accgaaacgg tttgcggcaa agttacgcaa tgttggattg ccgttatatt
      541 tgcccaacgg cgctgcgccg aatctttcac tcattctcgg cggcgctggt gcaaaactgg
      601 aagatatggc ggcagcgtat accgcgtttg ctcgccacgg caaggcaggc aaattgcgct
      661 tacagcctga cgatccgctg cttgaacggc ctttaatgtc gtcgggggcg gcgtggatca
      721 ttcgccggat tatggctgat gaagcgcaac ccttgccgga tagtgccttg ccgcgcgtcg
      781 ccccactggc atggaaaacg ggcaccagct atggctatcg tgacgcctgg gcgattgggg
      841 ttaacgctcg ctatgtcatt gggatctgga ctggcagacc tgacggcacg cccgttgttg
      901 gtcagtttgg ctttgccagt gccgtaccat tgttgaatca ggtcaataat atcttactgt
      961 cgcgcagtgc gaatctgccg gaagacccgc gcccgaactc agtcactcgt ggcgttatct
     1021 gctggccggg cggacagtct ttgccggaag gtgacggtaa ctgtcgccgc cgcctggcaa
     1081 cctggctgct ggatggcagc cagccgccaa cgctgttact gtcagagcag gaagggatta
     1141 atggcattcg cttccccatc tggctggatg acaaagggca gcgcgtcgcc gctgactgcc
     1201 cgcaggcgaa gcaagaaatg gtaaatgtct ggccgctacc gctggaaccc tggctgcccg
     1261 catcagaacg tcgcgctgtg cgcttaccac cggcctcgac aatttgcccg ccatacggtc
     1321 acgatgccca gctccctctg cagctgacag gcgttcgcga tggcgcgatt attaaacgtt
     1381 taccgggcgc agcggaagcg actttgccgt tgcaatcaag tggaggggca ggtgaacgct
     1441 ggtggtttct gaatggcgaa ccgttaactg aacgcgggcg caacgtgacc ctgcatttga
     1501 cggataaagg tgattatcaa ttgctggtga tggatgatgt ggggcaaatc gcgacagtga
     1561 aatttgtcat gcaatagtct gttttgtccc tgattgttgc taaaactcat tttattttaa
     1621 aaaaaatgtt accttcatca atagtcaacg gccctgttgc tcattataat ccgcgccatc
     1681 tcgtacgctg gtacagacaa caacagaaca atttacagag gtaaaaatgg ctattgaacg
     1741 tactttttcc atcatcaaac cgaacgcggt agcaaaaaac gtcattggta atatctttgc
     1801 gcgctttgaa gctgcagggt tcaaaattgt cggcaccaaa atgctgcatc tgaccgttga
     1861 acaggcacgt ggcttttatg ctgaacacga tggaaaaccg ttctttgatg gtctggttga
     1921 attcatgacc tctggcccga tcgtggtttc cgtgctggaa ggtgaaaacg ccgttcagcg
     1981 tcaccgcgat ctgctgggcg cgaccaatcc ggcaaacgca ctggctggta ctctgcgcgc
     2041 tgattacgct gacagcctga ccgaaaacgg tacccacggt tctgattccg tcgaatctgc
     2101 cgctcgcgaa atcgcttatt tcttcggcga aggcgaagtg tgcccgcgca cccgttaata
     2161 atttcgtaaa tgccgcgtgc aaacgtggca tccgtgcgcc agaatttgta caatgcagcg
     2221 cccccggacg agcagccgct caccggggcg tttctttttt caaccctcca ggggccataa
     2281 cgtgtaataa cgaggccgga atagcatatg tctgaacaat tagtcacacc tgaaaacgtc
     2341 accacgaaag atggaaaaat caacctgctg gatctcaacc gtcagcagat gcgggagttt
     2401 tttaaagatt taggtgaaaa aaccttccgc gccgatcagg tgatgaagtg gatgtatcac
     2461 tattgctgcg acaactttga tgagatgacc gacatcaaca aagtgttgcg cggcaaactg
     2521 aaagaggtgg cggaaatccg cgcaccggaa gtggttgaag aacagcgttc atccgacggc
     2581 accattaaat gggcgatcgc cgttggcgat cagcgcgtcg aaacggtgta tatcccggaa
     2641 gacgaccgtg ccacgctctg cgtctcttcg caggtggggt gtgcgctgga gtgtaaattc
     2701 tgttccaccg cccagcaggg ctttaaccgc aacctgcggg tatcggaaat tattggccag
     2761 gtgtggcgtg cggcgaaaat cgtcggcgca gcaaaagtca ccggtcagcg cccaatcact
     2821 aacgtcgtga tgatgggcat gggcgagccg ctgctcaacc tgaacaacgt cgttccggcg
     2881 atggaaatca tgcttgatga tttcggtttt ggcctgtcta aacgtcgcgt gacgctttcc
     2941 acttccggtg tagttccggc gctggataaa ctgggcgata tgatcgacgt tgcactggca
     3001 atttccctgc acgcgccgaa cgacgaaatt cgtgacgaaa ttgtgccgat caacaaaaag
     3061 tacaatatcg aaacgttcct tgctgcggtg cgccgttatc tggagaaatc caacgccaat
     3121 cagggccgag tcactattga atacgtgatg cttgaccacg ttaacgacgg cactgaacac
     3181 gcgcaccaac tggcggaact gctgaaagat acgccgtgta agatcaacct gatcccgtgg
     3241 aacccgttcc cggacgcgcc gtatggacgc agttcgaaca gccgtatcga ccgtttctca
     3301 aaagtgctga tgagctacgg tttcaccact attgtgcgta aaacgcgtgg tgatgatatc
     3361 gatgctgcct gtggtcagtt ggcgggcgat gttatcgacc gtacgaaacg tactctgcgt
     3421 aaacggatgc agggtgaagc catcgatatt aaagcggtct gatagccatt acgccacggt
     3481 tactttatgt accgtggcgt aatcttttat gaatgaagta ttaagttgca attgtctggt
     3541 caatattaac ggtgcgtttt tgctgacttt aaggcagtat gtaacgatgc aacagtaagt
     3601 tagcctcagt gaatgtgggc tttgtcacga gcacacagac ggtcttatac tgtatgataa
     3661 cggttaactt aacggatgtt tcgcggtgtg ggtgagcatg atggttcacc ggcatctcaa
     3721 ttctcattta aacgtacctg cagcgaatga atactgaagc cacgcacgac caaaatgaag
     3781 cacttactac cggcgctcgc ctgcgtaatg ctcgcgaaca actaggactt agtcagcagg
     3841 ccgttgccga gcgactttgc ctgaaggttt ccacggtacg cgacattgaa gaagataagg
     3901 cacccgccga tcttgcttca acattcctgc gcggatatat ccgctcttat gcgcgtctgg
     3961 tacatattcc agaagaagaa ctgctgccag ggctggaaaa gcaggctcca cttcgggctg
     4021 caaaagttgc gccaatgcag agtttttccc tcggtaaacg ccgcaaaaaa cgcgacggct
     4081 ggctaatgac cttcacctgg ctggtgttgt ttgtggttat cggcctgagc ggtgcctggt
     4141 ggtggcaaga ccacaaagct cagcaggaag agatcaccac tatggccgat caatcttcgg
     4201 cggaactgag cagtaatagc gagcaggggc agagtgttcc gttaaatacg tcgacaacta
     4261 cagacccggc tacaacgtcc acgccgccag cgtctgtgga tactaccgca accaacacgc
     4321 aaacacctgc cgtaactgcg ccagcaccag ctgttgatcc gcaacagaat gcggttgttt
     4381 cgccttcgca ggcaaatgtt gataccgccg cgaccccggt accaacggca gcaacaacgc
     4441 cagatggtgc tgcgccgttg ccaaccgatc aggctggcgt gaccacgccg gcggctgatc
     4501 cgaatgcgct ggtgatgaac tttactgccg attgctggct ggaggtcact gatgctaccg
     4561 gtaaaaaatt gtttagcggg atgcagcgta aagacggtaa tttgaattta accggccagg
     4621 caccgtataa actgaaaatt ggtgcgccag ccgcagtaca gatccagtat caagggaaac
     4681 ctgtcgatct gagtcgtttt atcagaacta accaggttgc gcgtctgacc ctcaatgccg
     4741 aacaatcacc ggcgcagtaa cagacgggta acgcgggaga tttttcatgc ataaccaggc
     4801 tccaattcaa cgtagaaaat caacacgtat ttacgttggg aatgtgccga ttggcgatgg
     4861 tgctcccatc gccgtacagt ccatgaccaa tacgcgtacg acagatgtcg aagcaacggt
     4921 caatcaaatc aaggcgctgg aacgcgttgg cgctgatatc gtccgtgtct ccgtaccgac
     4981 gatggacgcg gcagaagcgt tcaaactcat caaacagcgg gttaacgtgc cgctggtggc
     5041 tgacatccac ttcgactatc gcattgcgct gaaagtagcg gaatacggcg tcgattgtct
     5101 gcgtattaac cctggtaata tcggtaatga agagcgtatt cgcatggtgg ttgactgtgc
     5161 gcgcgataaa aacattccga tccgtattgg cgttaacgcc ggatcgctgg aaaaagatct
     5221 gcaagaaaag tatggcgaac cgacgccgca ggcgttgctg gaatccgcca tgcgtcatgt
     5281 tgatcatctc gatcgcctga acttcgatca gttcaaagtc agcgtgaaag cgtctgacgt
     5341 cttcctcgct gttgagtctt atcgtttgct ggcaaaacag atcgatcagc cgctgcatct
     5401 ggggatcacc gaagccggtg gtgcacgcag cggggcagta aaatccgcca ttggtttagg
     5461 tctgctgctg tcggaaggca tcggcgacac gctgcgtgta tcgctggcgg ccgatccggt
     5521 cgaagagatc aaagtcggtt tcgatatttt gaaatcgctg cgtatccgtt cgcgagggat
     5581 taacttcatc gcctgcccga cctgttcgcg tcaggaattt gatgttatcg gtacggttaa
     5641 cgcgctggag caacgcctgg aagatatcat cactccgatg gacgtttcga ttatcggctg
     5701 cgtggtgaat ggcccaggtg aggcgctggt ttctacactc ggcgtcaccg gcggcaacaa
     5761 gaaaagcggc ctctatgaag atggcgtgcg caaagaccgt ctggacaaca acgatatgat
     5821 cgaccagctg gaagcacgca ttcgtgcgaa agccagtcag ctggacgaag cgcgtcgaat
     5881 tgacgttcag caggttggaa aaataataac gtgatgggaa gcgcctcgct tcccgtgtat
     5941 gattgaaccc gcatggctcc cgaaacattg agggaagcgt tgagggttca tttttatatt
     6001 cagaaagaga ataaacgtgg caaaaaacat tcaagccatt cgcggcatga acgattacct
     6061 gcctggcgaa acggccatct ggcagcgcat tgaaggcaca ctgaaaaacg tgctcggcag
     6121 ctacggttac agtgaaatcc gcttgccgat tgtagagcag accccgctat tcaaacgtgc
     6181 gattggtgaa gtcaccgacg tggttgaaaa agagatgtac acctttgagg atcgcaatgg
     6241 cgacagcctg actctgcgcc ctgaagggac ggcgggctgt gtacgcgccg gcatcgagca
     6301 tggtcttctg tacaatcagg aacagcgtct gtggtatatc gggccgatgt tccgtcacga
     6361 gcgtccgcag aaagggcgtt atcgtcagtt ccatcagttg ggctgcgaag ttttcggtct
     6421 gcaaggtccg gatatcgacg ctgaactgat tatgctcact gcccgctggt ggcgtgcgct
     6481 gggtatcttc gaacacgtaa ctcttgagct gaattctatc ggttcgctgg aagcacgcgc
     6541 caattaccgc gatgcgctgg tggcattcct tgagcagcat aaagaaaagc tggacgaaga
     6601 ctgcaaacgc cgcatgtaca ctaacccgct gcgcgtgctg gattcaaaaa atccggaagt
     6661 gcaggcgctt ctcaacgacg ctccggcatt aggtgattat ctggacgagg aatctcgtga
     6721 gcattttgcc ggtctgtgca aactgctgga gagcgcgggg atcgcttaca ccgtaaacca
     6781 gcgtctggtg cgtggtctgg attactataa ccgtaccgtt ttcgagtggg tgactaacag
     6841 tctcggctcc cagggcaccg tgtgtgcagg cggtcgttat gacggtcttg tggaacaact
     6901 gggcggtcgt gcaacaccgg ctgtcggttt tgcgatgggc ctcgaacgtc ttgtattgtt
     6961 agtacaggcc gttaatccgg aatttaaagc cgatcctgtt gtcgatatat acctggtggc
     7021 ttcaggtgct gatacacaat ctgcggctat ggcattagct gagcgtctgc gtgatgaatt
     7081 actgggcgtg aaattgatga ccaaccacgg cggcggcaac tttaagaaac agtttgcccg
     7141 tgctgataaa tggggtgccc gcgttgctgt ggtgctgggt gagtctgaag tggctaacgg
     7201 cacagcagta gtgaaggatt tgcgctctgg tgagcaaacg gcagttgcgc aggatagcgt
     7261 agccgcgcat ttgcgcacgt tactgggtta aggaaggaga aggacagcgt ggaaatttac
     7321 gagaacgaaa acgaccaggt agaagcggtt aaacgctttt ttgctgaaaa tggcaaagca
     7381 ctggctgttg gggggatttt gggcgttggc gcactgactg gctggcgcta ctggaacagc
     7441 catcaggttg attctgcacg ctccgcttct cttgcctatc aaaatgcggt taccgcagtg
     7501 agcgaaggca aaccggatag catcccggcg gcggaaaaat ttgctgctga aaataaaaat
     7561 acttatggtg cgctggcttc tttggaactt gcgcagcaat ttgttgacaa aaatgaacta
     7621 gagaaagctg ccgcccagtt acaacagggg ctggcagaca cgagcgatga aaatctcaaa
     7681 gccgtgataa atctgcgtct tgctcgcgtt caggtacagc tcaagcaggc tgatgccgcg
     7741 ctgaaaaccc ttgataccat caaaggtgaa gggtgggctg ccattgttgc cgacttgcgt
     7801 ggtgaagcat tgctgagcaa aggtgataag caaggtgcgc gtagtgcatg ggaagcaggc
     7861 gtgaaaagcg atgttactcc ggcactgagc gaaatgatgc agatgaaaat taataatttg
     7921 tccatctgag agggacccga tgcaattgcg taaattactg ctgccaggac tgctttccgt
     7981 taccctttta agcggctgtt cgctgtttaa cagcgaagaa gatgtggtaa agatgtcccc
     8041 attgccaacc gttgaaaacc agtttacgcc gaccacggcg tggagcactt ccgttggtag
     8101 cggcattggc aacttctatt ccaatcttca tccggcactg gcggacaacg ttgtctatgc
     8161 agcggaccgc gctggtttgg taaaagcgct gaatgcggat gatggcaaag aaatctggtc
     8221 tgtcagcctg gccgagaaag atggctggtt ctctaaagag cctgcattac tttctggcgg
     8281 tgtgaccgtg tctggtgggc atgtctacat tggcagcgaa aaggcgcagg tttacgcgct
     8341 gaataccagc gatggtactg tggcatggca aactaaagtc gcgggtgaag cactttcgcg
     8401 cccggtggtc agcgacggtc tggtgttaat ccacaccagt aacggtcagt tacaagcgct
     8461 gaacgaagct gacggcgctg tcaaatggac agttaacctc gatatgcctt cgctctcttt
     8521 gcgtggcgag tctgcgccgg caacggcttt tggtgcggcc gtcgtgggtg gcgataatgg
     8581 tcgcgtcagc gcagtgctga tggaacaggg ccagatgatt tggcagcagc gtatttccca
     8641 ggcgaccggt tctaccgaaa ttgaccgtct gagcgatgtt gacacgactc ccgtcgttgt
     8701 taacggcgtt gttttcgcgc tggcctataa tggtaacctg acggcgcttg atctgcgcag
     8761 tggtcagatt atgtggaaac gcgaactggg ttcggtgaat gatttcatcg tcgatggcaa
     8821 tcgcatctat ctggtcgatc aaaatgaccg ggtgatggcg ttgaccattg atggcggcgt
     8881 tacgctgtgg gcacaaagcg atctgctgca tcgcctgctg acttctccgg tgctgtataa
     8941 tggcaacctg gtggtcggtg acagtgaagg ttatctgcac tggattaacg tcgaagatgg
     9001 tcgtttcgtt gcccagcaaa aagttgatag ttccggtttc cagactgaac cggttgccgc
     9061 tgacggcaaa ctgctgatcc aggcaaaaga cggaaccgtg tactctatta cacgttaatc
     9121 gtctctgtcg ttcactttga aaacggctcc tggacagggg ccgttttcct gtttttaaca
     9181 acgacgcgaa tatagcgtgc gttgtctgat gatttataaa aatgaggctt taaacatggt
     9241 acctgtggtc gcgcttgtcg ggcgccctaa cgtaggaaaa tccacgttat ttaaccgtct
     9301 aactcgcacc cgagatgcgc tggttgcgga tttcccgggt ctgactcgtg accgtaagta
     9361 cggtcgtgcg gaaattgaag gccgtgagtt tatctgtatt gataccggcg ggattgatgg
     9421 cacagaagac ggtgtagaaa cccgcatggc ggaacagtcg ctgctggcga ttgaagaagc
     9481 ggacgtcgta ctgtttatgg tggatgcgcg cgcgggcctg atgccggcag atgaagcgat
     9541 tgccaaacat ctgcgctccc gtgaaaaacc gaccttcctg gtggtaaaca aaactgacgg
     9601 tctggatccc gatcaggcag tggttgattt ctactcgctt ggtttaggtg aaatctaccc
     9661 gatcgccgcg tctcacggtc gtggcgtatt aagtctgctg gagcatgtgc tgctgccgtg
     9721 gatggaagat ctcgcaccgc aagaggaagt cgacgaagac gctgaatact gggcgcaatt
     9781 tgaagcggaa gagaacggcg aagaagaaga ggaagacgac ttcgacccgc aaagtctgcc
     9841 gatcaaactg gcgattgtgg gtcgtccgaa cgtaggtaag tctacactca ctaaccgtat
     9901 tcttggagaa gagcgcgttg ttgtttacga catgcctggc acgacgcgtg acagcatcta
     9961 catcccaatg gaacgcgatg gacgtgagta tgtgctcatt gacaccgctg gcgtacgtaa
    10021 acgcggcaaa atcaccgatg ctgtagagaa attctccgta atcaaaacgt tgcaggccat
    10081 tgaagacgcc aacgtggtga tgttagtgat tgatgcgcgc gaaggtattt ccgatcagga
    10141 tctctcgctg ctgggcttta ttctcaatag tgggcgctca cttgtcattg tggtgaataa
    10201 gtgggatggc ctgagccagg aagtgaaaga gcaggtgaaa gaaacgctgg acttccgtct
    10261 gggctttatc gattttgctc gtgtgcactt tatctctgcc ttgcacggca gtggtgttgg
    10321 taacttgttt gaatcagtac gtgaagcgta tgacagctcc acccgtcgtg tggggacctc
    10381 tatgctgacg cgcatcatga cgatggctgt tgaagatcac caaccgccgc tggtacgcgg
    10441 tcgtcgtgtg aagctgaaat atgcccacgc tggtggttat aacccgccga ttgtggtgat
    10501 tcacggtaat caggtgaaag acctgcctga ttcctacaag cgctacttga tgaactactt
    10561 ccgcaaatcg ctggacgtaa tgggatcgcc gattcgtatt cagttcaaag aaggggaaaa
    10621 cccgtatgcg aacaagcgta acaccctgac gccaacccag atgcgtaaac gtaagcgtct
    10681 gatgaagcac atcaagaaaa ataaataatt atccgtaagt attttatctg ccattccctc
    10741 tatgaatgta gagggaatgg ctaccggagg aattcatatg gaactgcatt gtccaaaatg
    10801 tcagcatgtg cttgatcagg ataacggcca tgcgcgttgc ccatcctgcg gggaatttat
    10861 tgaaatgaaa gctctttgtc cagactgcca tcaaccgttg caggtgttaa aagcctgcgg
    10921 tgcagtagat tacttctgtc agcacgggca tggcttgata tcgaaaaaac gcgttgaatt
    10981 cgtgctggct taatgaacct ttttacgcga tttttttacc ggctggatgt tttttacttc
    11041 actttctatc cagccgtctt ccagacgtgt ggttagcatt tcacccgttt taacttgctt
    11101 cacttttttc agtacattgc cgtcagtagc agtagtaacg ctgtatccac gcgccagcgt
    11161 tgacagtggg cttacggctt cgaggtgcgt tactgcatta ccgaaacgtt cacgcgtggc
    11221 gctaagctgt gcgcgcagga tttctgctaa acgatattcc agttgctgaa tgcgcgtttg
    11281 cgtgcgatga atcttcggct gtggattttg ctgattcagc cgctgtgtta atcgctgctg
    11341 ctgttgcccg gcacgcttaa gctgattttc cagcgcaaag ctcatccgtt tttgcaggcg
    11401 ttcaagcatg gtttgctggc gtgccagccg gagctgcgga tgctgctgct gcaagcgatg
    11461 atgaatctgc gtaaagcgac gtgtacggtt ggcgagataa taatccatcg ccatctccag
    11521 ccgttgacgg gccgattgca cctggcgcag taactcttgc tgattacggc tcactacttc
    11581 agcggcggca gacggcgttg gcgcacgcag atcggcaaca aaatcggcaa tggtcacatc
    11641 cgtctcatgc ccgacggcgc tgacgaccgg aatgcggctg gcaaaaatcg cccgcgctac
    11701 gcgttcgtcg ttaaaactcc ataaatcttc cagcgaaccg ccgccgcgcc caacgatcaa
    11761 cacgtcgcac tcattgcgct gattcgccag ttcaatggcg cgaacaattt gccccggcgc
    11821 gtcatcgccc tgaacggagg tggggtagat gatcaccggt agagaaggat cgcgacgttt
    11881 taacacatgc aaaatatcat gtagcgcagc accggttttt gaggtgatca caccaacgca
    11941 atgcgcaggg gagggaagtg gttttttgta ttgctgatcg aacaaacatt cagcctgcaa
    12001 cttcgctttg agctgttcgt acttctgttg cagcagccct tcaccggccg gctgcatact
    12061 ctcaacgatt atctggtagt cgccgcgcgg ctcgtagagc gtaatattgg cgcgaactaa
    12121 aacttgttgc ccatgctgcg gacggaaggt gacccggcgg ttgctgttgc ggaacatcgc
    12181 gcagcgtacc tgggcggtgt cgtctttgag tgtaaagtac cagtgaccgg aagccggttg
    12241 tgtgaaatta gagatttcgc cgctgatcca aacctgtccc atctcatgct caagcagcag
    12301 acgaaccgtt tgattcaggc gactaacggt aaaaattgca ggggattgag aaggtaacat
    12361 gtgagcgaga tcaaattcta aatcagcagg ttattcagtc tatagtaacc cgcccttcgg
    12421 ggatagcaag cattttttgc aaaaaggggt agatgcaatc ggttacgctc tgtataatgc
    12481 cgcggcaata tttattaacc actctggtcg agatattgcc catgctacgt atcgctaaag
    12541 aagctctgac gtttgacgac gttctcctcg ttcctgctca ttctaccgtt ctgccgaata
    12601 ctgctgacct cagcacccag ctgacgaaaa ctattcgtct gaatatccct atgctttccg
    12661 cagcaatgga taccgtaacg gaagcgcgcc tggctattgc tctggctcag gaaggcggta
    12721 tcggctttat ccacaaaaac atgtccattg aacgccaggc agaagaagtt cgccgtgtga
    12781 aaaaacacga atctggtgtg gtgactgatc cgcagactgt tctgccaacc acgacgctgc
    12841 gcgaagtgaa agaactgacc gagcgtaacg gttttgcggg ctatccggtc gttaccgaag
    12901 aaaacgaact ggtgggtatt atcaccggtc gtgacgtgcg ttttgttacc gacctgaacc
    12961 agccggttag cgtttacatg acgccgaaag agcgtctggt caccgtgcgt gaaggtgaag
    13021 cccgtgaagt ggtgctggca aaaatgcacg aaaaacgcgt tgaaaaagcg ctggtggttg
    13081 atgacgaatt ccacctgatc ggcatgatca ccgtgaaaga cttccagaaa gcggaacgta
    13141 aaccgaacgc ttgtaaagac gagcaaggcc gtctgcgtgt tggtgctgca gttggcgcag
    13201 gtgcgggtaa cgaagagcgt gttgacgcgc tggttgccgc tggcgttgac gttctgctga
    13261 tcgactcctc ccacggtcac tcagaaggcg ttttgcagcg tatccgtgaa acccgtgcta
    13321 aatatccgga tctgcaaatc atcggcggca acgtggcaac agctgcaggt gcacgcgctt
    13381 tggcagaagc tggttgcagc gcggttaaag tcggtatcgg ccctggttct atctgtacga
    13441 ctcgtatcgt tactggcgta ggtgttccgc agatcactgc cgttgctgac gcagttgaag
    13501 ccctggaagg caccggtatt ccggttatcg cagatggtgg tattcgcttc tccggcgaca
    13561 tcgccaaagc tatcgccgct ggcgcaagcg cggtgatggt aggttccatg ctggcgggta
    13621 ctgaagaatc tccgggtgaa atcgaactct accagggccg ttcttataaa tcttaccgtg
    13681 gtatgggttc cctgggcgcg atgtccaaag gttcctctga ccgttatttc cagagcgata
    13741 acgctgccga caaactggtg ccggaaggta tcgaaggtcg cgtagcttat aaaggtcgcc
    13801 tgaaagagat cattcaccag cagatgggcg gcctgcgctc ctgtatgggt ctgaccggct
    13861 gtggtactat cgacgaactg cgtactaaag cggagtttgt acgtatcagc ggtgcgggca
    13921 ttcaggaaag ccacgttcac gacgtgacca ttactaaaga gtccccgaac taccgtctgg
    13981 gctcctgatt ctcttcgccc gatttcatgt cgggcgattt atattatctg tttcacttgc
    14041 ctcggaataa gcgtcaatga cggaaaacat tcataagcat cgcatcctca ttctggactt
    14101 cggttctcag tacactcaac tggttgcgcg ccgcgtgcgt gagctgggtg tttactgcga
    14161 actgtgggcg tgggatgtga cagaagcaca aattcgtgac ttcaatccaa gcggaattat
    14221 tctttccggc ggcccggaaa gtactactga agaaaacagt ccgcgtgcgc cgcagtatgt
    14281 ctttgaagca ggcgtaccga tattcggcgt ttgctatggc atgcagacca tggcaatgca
    14341 gttgggcggt cacgttgaag cctctaacga acgtgaattt ggctacgcgc aggtagaagt
    14401 cgtaaacgac agcgcactgg ttcgcggtat cgaagatgcg ctgaccgcag acggtaaacc
    14461 gctgctcgat gtctggatga gccacggcga taaagttacc gctatcccgt ccgacttcgt
    14521 caccgtagcc agcaccgaaa gctgcccgtt tgccattatg gctaacgaag aaaaacgctt
    14581 ctatggcgta cagttccacc cggaagtgac tcacacccgc cagggtatgc gcatgctgga
    14641 gcgttttgtg cgtgatatct gccagtgtga agccctgtgg acgccagcga aaattatcga
    14701 cgatgctgta gctcgcatcc gcgagcaggt aggcgacgat aaagtcatcc tcggcctctc
    14761 tggtggtgtg gattcctccg taaccgcaat gctgctgcac cgtgctatcg gtaaaaacct
    14821 gacttgcgta ttcgtcgaca acggcctgct gcgtctcaac gaagcagagc aggttctgga
    14881 tatgtttggc gatcactttg gtcttaacat tgttcacgta ccggcagaag atcgcttcct
    14941 gtcagcgctg gctggcgaaa acgatccgga agcaaaacgt aaaatcatcg ggcgcgtttt
    15001 cgttgaagta ttcgatgaag aagcgctgaa actggaagac gtgaagtggc tggcgcaggg
    15061 caccatctac cctgacgtta tcgaatctgc ggcttctgca accggtaaag cacacgtcat
    15121 caaatctcac cacaacgtgg gcggcctgcc gaaagagatg aagatgggcc tggttgaacc
    15181 gctgaaagag ctgttcaaag acgaagtgcg taagattggt ctggagctgg gcctgccgta
    15241 cgacatgctg taccgtcatc cgttcccggg accaggcctt ggcgttcgtg ttctgggtga
    15301 agtgaagaaa gagtactgtg acctgctgcg ccgtgctgac gccatcttca ttgaagaact
    15361 gcgtaaagcg gacctgtacg acaaagtcag ccaggcgttc accgtcttcc tgccagtacg
    15421 ttccgttggc gtaatgggcg atggtcgtaa gtatgactgg gttgtctctc tgcgtgctgt
    15481 cgaaaccatc gactttatga ccgcacactg ggcgcatctg ccgtacgatt tcctcggtcg
    15541 cgtttccaac cgcattatca atgaagtgaa cggtatttcc cgcgtggtgt atgacatcag
    15601 cggcaagccg ccagctacca ttgagtggga atgattttga tccctgcact atgaatgaac
    15661 aaaaccctcc gttactacag agggtttttt atcttcaaga atcataggct tgaagttact
    15721 aacatcgatt aattaaacca gctgtccgat ttgttctctt ctgctttgcc cacgcttttc
    15781 atcagatcgc gaccgccttc agtcatattt ctgtttgcgt cagcttcaga ttgcaccaca
    15841 tcggtttgcg cagctttgtg cttcagttcc tgatcgataa attcgttttc gcgcttaacg
    15901 cgggcttctt ctttcgccag cgccagtttt tgtttctgaa tctctaagct gcgtagctca
    15961 tcttcataac tttgatcgcg ttttttgtcc gcagtggctt cggcgtccag tttatcctga
    16021 cgagctttct tatttgccgc tgccgttgcc gctcttttgt tcgcggcggc ctgggcattt
    16081 gcgcgacgtt gcttctcttg ctggatttcc ctgttgcgct ccgcgaccca ttcgtcatgc
    16141 tgcctttgct cttcattttt accttgctgt tccgcttctg cgacagcaga gagttgatcc
    16201 tgcaatgatg aggctatagc cggatagctt aaggaggcta agatggcgca aagaaaaact
    16261 ttcttcatga ctcctcctga ttattagctc ttttcaggac atttggtatt tggctgaata
    16321 cgcgtttcgt tatacgttgt ggtaataaca acggctaaac ctgtcgtaaa ctggcactct
    16381 ttaccctcct gggtggaggt atacactttg gtgccttcct tatatgttaa agaaacacct
    16441 tccactaagg ttttatcatt caccatagaa cccgctgccg cgcctacagc tccgccgcca
    16501 actgcccctg ccgtcgttcc ggaattgctg ccagaaccga cgttgtggcc gataacaccg
    16561 ccagcgactg cgccaataag cgcgccgaag gcttgtgcgt tccgtttatt ttgggagttg
    16621 tctacggcaa cttttgcggg aagaatggaa ataatattaa cggttttagt ttcttgtttg
    16681 gtattcagtt gatcggtttg ataaacatcg gcggcatgat cgtcagcatt tgactggcat
    16741 cctgccagag tgaatgacgc taacattgcc acaggcagaa gacatttttt aaatttcatc
    16801 actattcctt gttatacaca ttacgaaaaa taagacattt aagatcatca aaagaggaaa
    16861 gtaaatgtat tgcgtataaa gggatagatt aaatttatta aaattcacgc agggaataat
    16921 ttataaaaat atatcattac aacatatata ttaattttat atttatttcg cagccaaaca
    16981 ggataaacat cagtcaggac aaagacgtaa aatttaagat gaaagaatat ctgccgcttt
    17041 gataatccca aagagcaaaa gcatccgaca gcgaagtgcc gttatgttct gtgaaagtgg
    17101 agaaggtaac cgctaacgtt gtaccggtga ttcgacggag agtgtggatt gctgagcttc
    17161 ttttttgctc tgatggtgct gccaggcacc gacggacgag tagataaaac ggccaaagaa
    17221 gaagataaag ctgatgagca gtacgatacg ggtcatgcga ctgttaaatc ggtgtcgttt
    17281 tcgcatacta gttgcctgac tcacaaaagg ttccttgaag tatgtcccac gctgtggacg
    17341 gtacttacat taaggcacaa caggacaaaa tggtcaattc ttcgttatgt aaaaaagcgt
    17401 cagtggatac atattttaat gttatggaag ttaatttaat tatttacaat gatgatgtaa
    17461 taatgatgaa ttaacatgat aaacgtaata attcattaat cagggttatt ttgtttgata
    17521 tatatcaatt ggaactttca tatgacactt agaatcaatg ctctctatag tgatgaataa
    17581 tcatcattcg aagtcaggtg ggatgcctgt ctgaatacac ctccttcagg atgtggggga
    17641 ttctgctgag catctatgaa actgaatgca acttatataa aaatacgtga taaatggtgg
    17701 gggcttccgc tgttcctgcc ttctttaatc ttgcccattt tcgcccacat taatactttc
    17761 gcgcatattt cttccggtga ggtttttctc ttttatctgc ctttggcact gatgatcagc
    17821 atgatgatgt ttttcagctg ggcggcattg ccagggatca ccttagggat ttttgtccgc
    17881 aaatatgcag agctgggttt ttacgaaacg ctctcattaa cggctaattt tattatcatt
    17941 atcattctct gttggggcgg ttacagggtc tttactcccc ggcgtaacaa cgtttcacat
    18001 ggtgataccc gtttaatttc ccagcgtata ttctggcaga tagtgtttcc tgcaacgctg
    18061 tttctgatac ttttccagtt tgctgcattt gtaggattac tggcgagcag agaaaatctg
    18121 gtcggtgtca tgccttttaa cctcgggact ttaatcaatt atcaggcctt actggtgggt
    18181 aatctgatcg gtgtcccgct gtgctacttc atcattcggg tagtgcgaaa tccattttat
    18241 ttacgtagct attattcgca attaaaacag caggttgatg ccaaagtcac caaaaaagag
    18301 ttcgcgctct ggctactggc attaggtgct ttattgttgc tgttatgcat gccgttaaat
    18361 gaaaaaagca caatttttag caccaattat accttgtcat tattgctgcc cctgatgatg
    18421 tggggagcaa tgcgctatgg ttataagctg atttcgctgc tctgggcggt cgtgttgatg
    18481 atcagcatcc acagctatca aaattacatt cccatttatc ctggctatac cacgcagctg
    18541 accataacct cctccagtta tctggtattc tcttttattg tcaattatat ggctgtactg
    18601 gcaactcgtc agcgagcggt agtcagacgc attcagcggc ttgcgtatgt ggacccggtg
    18661 gttcatctgc caaatgttcg cgccctgaat cgcgcgttgc gtgatgcccc ctggtctgcg
    18721 ctttgttatt tacgcatccc tggcatggaa atgctggtta agaactatgg catcatgctg
    18781 cggattcaa
//
LOCUS       DAJTYH010000080        18670 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTYH010000080 DAJTYH010000000
VERSION     DAJTYH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(139..3654)
                     /locus_tag="OO609_002781"
     CDS             complement(139..3654)
                     /locus_tag="OO609_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6634138.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO609_002782"
     CDS             complement(4107..5369)
                     /locus_tag="OO609_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6634139.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO609_002783"
     tRNA            complement(5567..5642)
                     /locus_tag="OO609_002783"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO609_002784"
     CDS             complement(5748..6455)
                     /locus_tag="OO609_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6634140.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO609_002785"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO609_002785"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6634141.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO609_002786"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO609_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6634142.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO609_002787"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO609_002787"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6634143.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO609_002788"
     CDS             complement(11639..11914)
                     /locus_tag="OO609_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6634144.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO609_002789"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO609_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6634145.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO609_002790"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO609_002790"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6634146.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO609_002791"
     CDS             13874..14200
                     /locus_tag="OO609_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6634147.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO609_002792"
     CDS             14384..15103
                     /locus_tag="OO609_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6634148.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO609_002793"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO609_002793"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6634149.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO609_002794"
     CDS             16442..17404
                     /locus_tag="OO609_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6634150.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18670)
                     /locus_tag="OO609_002795"
     CDS             complement(17505..>18670)
                     /locus_tag="OO609_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR6634151.1"
                     /translation="TVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVM
                     VGRVPGSLGHVNVLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSS
                     CITGLLIPPSNVLIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPL
                     SERATFAQACKAFLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYRE
                     VKWRHLPKLILESVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIIL
                     LLINIVLLIVGIFMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPV
                     GSALFVGCSISGVKIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4844 a         4553 c         4618 g         4655 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga
//
LOCUS       DAJTYH010000081        18049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTYH010000081 DAJTYH010000000
VERSION     DAJTYH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            445..1128
                     /locus_tag="OO609_002796"
     CDS             445..1128
                     /locus_tag="OO609_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6634152.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1172..5834
                     /locus_tag="OO609_002797"
                     /pseudo
     CDS             1172..5834
                     /locus_tag="OO609_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6202..7857
                     /gene="lldP"
                     /locus_tag="OO609_002798"
     CDS             6202..7857
                     /gene="lldP"
                     /locus_tag="OO609_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6634153.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7857..8633
                     /gene="lldR"
                     /locus_tag="OO609_002799"
     CDS             7857..8633
                     /gene="lldR"
                     /locus_tag="OO609_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6634154.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8630..9820
                     /gene="lldD"
                     /locus_tag="OO609_002800"
     CDS             8630..9820
                     /gene="lldD"
                     /locus_tag="OO609_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6634155.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10016..10489
                     /gene="trmL"
                     /locus_tag="OO609_002801"
     CDS             10016..10489
                     /gene="trmL"
                     /locus_tag="OO609_002801"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6634156.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10542..11363)
                     /gene="cysE"
                     /locus_tag="OO609_002802"
     CDS             complement(10542..11363)
                     /gene="cysE"
                     /locus_tag="OO609_002802"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6634157.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11443..12462)
                     /gene="gpsA"
                     /locus_tag="OO609_002803"
     CDS             complement(11443..12462)
                     /gene="gpsA"
                     /locus_tag="OO609_002803"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6634158.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12462..12929)
                     /gene="secB"
                     /locus_tag="OO609_002804"
     CDS             complement(12462..12929)
                     /gene="secB"
                     /locus_tag="OO609_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6634159.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12992..13243)
                     /gene="grxC"
                     /locus_tag="OO609_002805"
     CDS             complement(12992..13243)
                     /gene="grxC"
                     /locus_tag="OO609_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6634160.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13385..13816)
                     /locus_tag="OO609_002806"
     CDS             complement(13385..13816)
                     /locus_tag="OO609_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6634161.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14061..15605
                     /gene="gpmM"
                     /locus_tag="OO609_002807"
                     /gene_synonym="pgmI"
     CDS             14061..15605
                     /gene="gpmM"
                     /locus_tag="OO609_002807"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6634162.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15615..16898
                     /gene="envC"
                     /locus_tag="OO609_002808"
     CDS             15615..16898
                     /gene="envC"
                     /locus_tag="OO609_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6634163.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16902..17861
                     /locus_tag="OO609_002809"
     CDS             16902..17861
                     /locus_tag="OO609_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6634164.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17848..18049)
                     /locus_tag="OO609_002810"
                     /pseudo
     CDS             complement(17848..18049)
                     /locus_tag="OO609_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4546 a         4663 c         4674 g         4166 t
ORIGIN      
        1 attcactccc tttacttcga tttaccgctg gttgatattc tgaaaatgca tttttaccga
       61 aatagccatc tataaacaat aaaaatatat agaagatatc atttgttaat taacaagcat
      121 ttacgtctct ttttttcgga tttaattgtg aaattttaat catcatattc acaaaatcaa
      181 ttatctgtga aatttttccc agacatggtt tctttcatat ttgacattta ttaataatca
      241 tcattagaat ccacgaaaaa taatatattc atacaaacac agacatcttt tgaaacgaat
      301 gatacatttt tatgatgaat gcttaaaaca atatttaatc gacacacatc actgtcatta
      361 atgtaaagca acatagctca ccaataatac aatggacaac tctaccggca ttgagcgctg
      421 gtcggaggaa ataaggattt ttacatgaac ttgaagaaga tttttttcag tgctgtcact
      481 gtttcagttc tctgcgcatt aacgggatgt gactacattg aagaaggcaa gccagaaagt
      541 agtttactga aacaacaaga agaacataat aataaaattg tgctacttga aaaacaacaa
      601 gcacagctaa agagtcaact cgaaactatt caaaaacaac aaactggcat aataaacagt
      661 acaaagactt taactcacgt aattaaatcc gttaaagacc aacaaaatac ttttattttt
      721 accgagttta acccagcaaa aacgaagtat ttcatcctga acaacggttc ggtggcttta
      781 gcgggtcgag tgttatccat tgacgccaca gagaatggta gtgttattca tatctcactg
      841 gtcaacttat taagtacacc tatctcaaac attggtttta atgcgacatg gggtggtgaa
      901 aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat tacttttcaa cacatcaatg
      961 aaatccacat tgaaattatt accaggtcaa tggcaagaca ttaatttgac cctgaagggt
     1021 gtatcgccca ataacctggg atatctgaag ttagccatca acatggaaaa tattcagttc
     1081 gacaatcttc cctctgctga aaatcggcag aaaagaagca aaaaataaat gcagttagta
     1141 cgcttataac aaggaatagc gagcatcatt aatgaacaaa atatttaaag ttatctggaa
     1201 ccctgcgaca gggaattata ctgttaccag cgaaacggca aaaagccgtg gcaagaaatc
     1261 tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt ggaatgttgt cgtcgtttgg
     1321 ggcattggcg aatgccggga atgacaacgg tcagggtgtt gattacggta gtggatcagc
     1381 tggcgacggc tgggttgcta taggcaaagg ggcgaaagca aatactttta tgaacaccag
     1441 tggttccagt actgctgtgg gttatgacgc tatagctgaa ggccaatata gctctgccat
     1501 cgggtcaaaa acccatgcga ttggtggtgc atcaatggcc tttggggtta gtgcaatatc
     1561 agaaggcgat agaagtatag cactgggtgc ctcttcgtat tcattgggcc aatactcaat
     1621 ggccctcggc cgttattcaa aagcattggg taaattgtct attgctatgg gggactcttc
     1681 caaagcggaa ggagcaaacg ccattgccct ggggcgtagc agtgtagcta gcggtactga
     1741 cagcctcgca tttggcagac aatcacttgc cagcgcagcg aacgctattg cgataggtgc
     1801 tgagaccgaa gccgctgaaa atgcaactgc tattggcaat aatgcgaagg caaaagggac
     1861 taatagcatg gcaatggggt tcggaagcct tgccgataaa gtcaatacta tcgcattagg
     1921 aaatggcagc caggctctgg cagataatgc aatcgccata ggccagggca acaaagctga
     1981 tggcgtggat gccatcgctc tgggtaatgg tagccagtcg agaggcttaa acaccattgc
     2041 cttaggcaca gccagtaatg caactggtga taagagtctt gcgcttggta gtaatagcag
     2101 tgccaacggt attaactctg tcgcgctggg cgcagattcc attgcggatt tagacaatac
     2161 cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt aatgtgaaaa atggcgcgat
     2221 caagtctgac agttacgatg ccattaatgg ttcacagctt tatgccatta gcgactcggt
     2281 agcaaaaaag gcttggagga ggggctgcag tagatgttga tgacggtact gttacagcac
     2341 caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc gctgtacttg
     2401 atgaaaacac cctgcaatgg gaccaaacca aaggcaaata cagcgctgct catggtacta
     2461 gtagcccaac tgccagcgta atcaccgatg ttgcggatgg cacgatttca gcctccagta
     2521 aggatgcggt taacggttcc caactgaaag ctaccaatga cgatgtcgaa gccaacacca
     2581 ccaatatcac caacctgacg gattccgttg gtgaccttca ggctgatgcc ctgctctgga
     2641 acgaaactaa aaaggcattc agtgcagctc acggccagga taccaccagc aaaatcacca
     2701 acgttaaaga tgccgacctg acggctgaca gcactgatgc tgttaacggc tctcagctga
     2761 aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat cgccaataac acttccaata
     2821 ttgccactaa caccaccaac atctctaacc tgactgagac ggtgactaat cttggtgagg
     2881 atgcgctgaa atgggataag gacaatggtg tattcacggc agctcatggc accgagacca
     2941 ccagcaaaat aaccaacgtt aaagatggcg acctgacgac tggcagcacc gatgccgtta
     3001 acggctctca gctgaaaacc accaacgatg ccgtggcgac gaataccacc aatatcgcca
     3061 ctaacaccac caacatctct aatctgactg agacggtgac taatcttggt gaggatgcgc
     3121 tgaaatggga taaggacaat ggtgtcttca ctgcagctca tggcaacaat accgccagca
     3181 aaatcaccaa tatcctggac ggcacagtca ctgcaaccag ttccgatgcc attaacggta
     3241 gccagcttta tgacttaagc agcaatatcg ccacctactt cggcggcaat gcttctgtga
     3301 atactgacgg tgtgtttacc ggtccaacct acaaaatcgg tgaaacaaat tattataacg
     3361 tcggcgatgc actggctgcg attaactcct catttagcac gtctctcggc gatgctctgc
     3421 tttgggatgc caccgcaggt aaattcagtg ccaaacacgg tactaatggt gacgcaagcg
     3481 tgatcactga tgtcgcagat ggtgaaattt cagactccag ttctgacgca gtaaacggct
     3541 cacaactcca cggcgtgagc agttatgttg ttgatgcgct ggggggtggt gccgaagtca
     3601 atgcagacgg caccatcact gcgccgacgt acaccattgc taatgctgat tacgataatg
     3661 tcggtgatgc cctgaatgct atcgatacca ctcctgacga cgctctgctc tgggatgcgg
     3721 acgccggtga aaatggtgca tttagcgccg ctcacggaaa agataaaact gccagtgtaa
     3781 tcactaacgt cgctaacggt gcaatctctg ctgccagcag cgacgcgatt aacggctcac
     3841 aactctatac caccaataag tacatcgctg atgcgctggg tggtgacgca gaagtcaacg
     3901 ctgacggcac catcaccgca ccgacttaca ccattgcgaa cgccgagtac aacaacgtcg
     3961 gtgacgccct ggatgcgctt gatgataacg ccctgctgtg ggataagact gccaatggcg
     4021 gtgctggagc ctacaatgcc agccatgacg gtaaagccag catcatcact aatgtcgcta
     4081 atggcagtat tagtgaggac agtaccgatg cagtgaacgg ttctcagttg aatgcgacga
     4141 atatgatgat tgagcagaac acccaaatta tcaatcagct cgctggtaac accgacgcaa
     4201 cctatatcga agaaaatggc gctggtatca actacgttcg tactaacgac aacgatttag
     4261 cctttaacga tgcaagcgcc tctggtgtcg gcgctacagc tgtaggttat aacgctgtcg
     4321 cgtctggtgc cagcagcgta gccattggtc agaacagcag cagcaccgtt gataccggta
     4381 ttgcgctggg tagcagctcc gtttccagcc gtgtgattgc caaaggttct cgtgacacta
     4441 gcgtaacgga aaatggcgtg gtgattggtt atgacaccac tgacggcgaa ctgctaggtg
     4501 cattgtcaat tggtgatgac ggtaaatacc gccaaatcat caacgtagct gatggttcag
     4561 aagcccatga cgccgttacg gttcgccagt tgcagaacgc tattggagcg gtcgccacta
     4621 cgccaaccaa gtactttcac gccaactcaa cggcagaaga ctcactggcc gttggtgaag
     4681 actcgctggc aatgggtgcg aaaactgtcg ttaatggtaa tgcaggtatt ggtatcggtt
     4741 tgaacactct ggttctggct gatgcgatca acggcattgc tatcggcagt aacgcacgcg
     4801 caaaccatgc aaacagtatc gcaatgggta acggttctca gaccacccgt ggtgcacaga
     4861 ctggctacac cgcctacaac atggacgcac cgcagaactc tgtaggtgag ttttctgtcg
     4921 gtagtgaaga cggtcaacgt cagatcacaa acgtcgcagc tggttcagcg gataccgatg
     4981 cggttaacgt gggtcagttg aaagtcactg atgagcgcgt agcgcaaaat acccagagca
     5041 ttactaacct gaacaatcag gtcactaatc tggatactcg cgttactaat atcgaaaacg
     5101 gtattggcga cattgtcacc accggtagca ccaagtactt caagaccaac accgatggcg
     5161 tagatgccaa cgcccagggt aaagatagcg ttgctattgg ttctggttcc attgctgccg
     5221 ctgacaacag cgtcgcactg ggtaccggtt ccgttgcaga ggaagaaaat acaatctctg
     5281 taggttcttc tactaaccaa cgccgtatca ccaacgtagc tgccagtgtt aatgccaccg
     5341 atgcggttaa cgtgtcgcag ctgaaatctt ctgaagcagg cggagtgcgc tacgacacca
     5401 aagctgatgg ttctatcgac tatagcaata tcaccctcgg tggtggcaac ggcagtacga
     5461 ctcgtatcag caacgtctcc gctggcgtca acaacaacga cgcggtgaac tacgcgcagt
     5521 tgaagcaaag cgcgcaggaa acgaagcaat acaccgatca gcggatggtt gagatggata
     5581 acaaactgtc taaaactgaa agcaagttga gcggtggtat cgcttctgca atggcaatga
     5641 ccggtctgcc gcaggcttat acaccgggtg ccagcatggc ttctattggt ggcggtactt
     5701 acaacggtga atcggcagtt gctttaggtg tatcgatggt gagcgccaat ggtcgttggg
     5761 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
     5821 gtattcagtg gtaatgatcc attaagttag tgtgactaag ggcaaacgcc aaattgcctg
     5881 atgcgttacg cgcatcaggc aagataaggc gctccgggtc aacaatctaa gggcaattct
     5941 ctgatgagga ttgccctttt ctttaccaga catctccccc cacaagaatt ggccctacca
     6001 attcttcgct tatctgacct ctggttcaca attccccaat taaaactcac atcaatattg
     6061 ccaatacata acatttagtt aaccattcat tgtcattatc cctacacaac acaattggca
     6121 gtgccacttt tacacaacgt gtgacaagga gatgagcaac agactcatta cacgatgtgc
     6181 gtggactcta ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat
     6241 atctggcttt ccagtctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt
     6301 aagctcaaac tgaaaggata cgtcgccgcc tcatggacgg tagcaatcgc ccttgccgtg
     6361 gctttgctgt tctataaaat gccggtcgct aacgcgctgg cctcggtggt ttatggcttc
     6421 ttctatggtt tgtggcccat cgcgtggatc attattgcag cagtgttcgt ctataagatc
     6481 tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta ttctttcgat aacccctgac
     6541 cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg ctttccttga aggagccgca
     6601 ggctttggcg caccggtagc aattaccgcc gcattgctgg tcggcctggg ttttaaaccg
     6661 ctgtacgccg ccggactgtg cctgattgtt aacaccgcgc cagtggcatt tggcgcgatg
     6721 ggcattccaa tcctggttgc cggacaagta acaggcatcg acagctttga gattggccag
     6781 atggtggggc gtcagctacc gtttatgacc attatcgtgc tgttctggat catggcgatt
     6841 atggacggct ggcgcggtat caaagagacg tggcctgcgg tcgtggttgc aggcggctcg
     6901 tttgccatcg ctcagtacct tagctctaac ttcattgggc cggagctgcc ggacattatc
     6961 tcttcgctgg tatcactgct ctgcctgacg ctgttcctca aacgctggca accagttcgc
     7021 gtattccgct tcggtgactt gggggcgtca caggttgata tgacgctggc ccacaccggt
     7081 tacaccgcgg gtcaggtgtt acgtgcctgg acaccgttcc tgttcctgac cgctaccgta
     7141 acactgtgga gcatcccgcc gtttaaagcc ctgttcgcat cgggtggtgc gctgtatgag
     7201 tgggtgatca acattccggt gccgtacctc aataaactgg ttgcccgtat gccaccagtg
     7261 gtcagcgagg ctacagccta tgccgccgtg tttaagtttg actggttctc tgccaccggc
     7321 accgccattc tgtttgctgc actgctctcg attgtctggc tgaagatgaa accgtctgac
     7381 gctatcagca ccttcggcag cacgctgaaa gaactggctc tacccatcta ctccatcggc
     7441 atggtgctgg cgttcgcctt tatctcgaac tattccggac tgtcatcaac actggcactg
     7501 gcactggcgc acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggctgggg
     7561 gtattcctga ccgggtcgga tacctcatct aacgccctgt tcgccgcgct gcaagccacc
     7621 gcagcacaac aaattggcgt ctctgatctg ttgctggttg ccgccaatac caccggtggc
     7681 gtcaccggta agatgatctc cccgcaatct atcgctatcg cctgtgcggc ggtaggcctg
     7741 gtgggcaaag agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt
     7801 atggtgggcg tgatcaccac gcttcaggct tatgtcttaa cgtggatgat tccttaatga
     7861 ttgttttacc cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg
     7921 aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg ccaactggcg atgcaactcg
     7981 gcgtatcacg taattcacta cgcgaggcgc tggcaaaact ggtgagcgaa ggcgtgctgc
     8041 tcagtcgacg cggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa
     8101 acatcgtcca gccgctaaaa acgctgatgg ccgatgatcc ggattacagt ttcgatattc
     8161 tggaagcccg ctacgccatt gaagccagca ccgcatggca tgcggcaatg cgcgccacac
     8221 ctggcgacaa agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggata
     8281 tcgcctcaca agcggacgtt cgttttcatc tggcgattgc cgaagcctca cataacatcg
     8341 tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca atcctcagtg aagcatagcc
     8401 gtcagcggat gtatctggtg ccaccggttt tttcacaact gaccgaacaa catcaggctg
     8461 tcattgacgc catttttgct ggtgatgccg acggggcgcg taaagcaatg atggcgcacc
     8521 ttagttttgt tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc cacgcacgga
     8581 ttacccgcct gcccggtgag cataatgagc attcgaggga gaaaaacgca tgattatttc
     8641 cgcagccagc gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgttccacta
     8701 tatggatggt ggtgcatatt ctgaatacac gctgcgccgc aacgtggaag atttgtcaga
     8761 agtggcactg cgccagcgta ttctgaaaaa catgtccgac ttaagcctgg aaacgacgct
     8821 gtttaatgag aaattgtcga tgccggtagc actggcaccg gtgggtttgt gtggcatgta
     8881 tgcgcgtcgt ggcgaagttc aggcagccaa agcggcggac gcgcatggca ttccgtttac
     8941 tctctcgacg gtttccgttt gcccgattga agaagtcgcg ccagccatca agcgcccaat
     9001 gtggttccag ctttatgtac tgcgcgatcg cggctttatg cgtaacgcgc tggagcgagc
     9061 aaaagcagcg ggttgttcga cgctggtttt caccgtggat atgccgacac cgggcgcacg
     9121 ctaccgtgat gcgcattcag gtatgagcgg cccaaatgcg gcaatgcgcc gctacttgca
     9181 ggcggtgacg catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cacatgattt
     9241 aggtaatatc tcagcttatc tcggcaaacc gaccggactg gaagattaca tcggctggct
     9301 ggggaataac ttcgatccgt ccatctcatg gaaagacctt gagtggatcc gcgatttctg
     9361 ggatggcccg atggtgatca aagggatcct cgatccggaa gatgcgcgcg atgcagtacg
     9421 ttttggtgct gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtact
     9481 ctcttccgct cgtgcgttgc ccgctattgc agatgcggtg aaaggtgata tcgccattct
     9541 ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga
     9601 caccgtactg ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc aggcgggtgt
     9661 agctaacctg ctaaatctga tcgaaaaaga gatgaaagtg gcgatgacgc tgactggcgc
     9721 gaaatcgatc agtgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc
     9781 tacggcactg gctccgatgg cgaaagggaa tgcggcatag tcgtttgcgc cccctcaccc
     9841 taaccctctc cctcagggag aggggaccgt tcggcgctgt atgtactccc tcactctgaa
     9901 acgacaccgc actctttttt tctccctcgc ccctccgggg agggccgggg tgaggggaaa
     9961 aggccgcacg gttcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
    10021 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg
    10081 cgctaatacc ggctttcgtc tacatatcat cgaaccgatg ggatttgcct gggacgacaa
    10141 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
    10201 ctatcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg
    10261 tacgcctgct cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga
    10321 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
    10381 tccgatggtg ccggacagcc gcagcatgaa tctgtccaat gcggtgtcgg tagtggtgta
    10441 tgaagcctgg cggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc
    10501 cggatagatg attacatcgc atccggcacg atcacaggac attagatccc atccccatac
    10561 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
    10621 tctggtttac cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt
    10681 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgagg
    10741 attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca
    10801 gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc
    10861 gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg gccaattttt
    10921 gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
    10981 aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
    11041 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
    11101 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
    11161 tcaaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
    11221 atgtagctca gtgcactgcc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
    11281 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
    11341 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
    11401 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtgac tgctgcgctc
    11461 gtccttgcgt gcacgaccta ataatgtcaa tgctgcctcg cgcgcgtttt ttccgcaata
    11521 taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc
    11581 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
    11641 ttgtacatcc atgccctgac cgagcatcat accaaaacgg cggttacgcg actggttgtc
    11701 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
    11761 acccagcgcc gcaccaagac gcgacatttc agccagccca cgggtgatca gcgccgtacg
    11821 cgcattcgca ccaaaaccga taccgtcaga catccccgcg ccaatggcaa tgacgttttt
    11881 caccgcgccg ccaagctgca cgccaatgaa atccgggttg ctgtaaacgc ggaaactttt
    11941 gccgcagtgc agcagctgct ggagatcatc ggcaaaggtc tgatcggtcg aggccagcga
    12001 aatagctgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
    12061 cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc
    12121 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
    12181 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacgactggc
    12241 tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg
    12301 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
    12361 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
    12421 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
    12481 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc
    12541 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga
    12601 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
    12661 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
    12721 tcgcccaagg aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt
    12781 tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg
    12841 tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa
    12901 gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
    12961 tactgcgcaa tgtaatacca acgttcacac gttatttcag caggggatcc agtccaccac
    13021 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
    13081 ggggaaccgt ggtgcaaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat
    13141 cgatcggcaa ctcctggaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
    13201 aatacgggca ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt
    13261 tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat
    13321 aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg
    13381 taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca acgccctctt
    13441 tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact
    13501 gcatgccaga accgtctacc acgataaccg atttgtcttt gtgcttctca agctcaccaa
    13561 cattgttggc tttgatttcg ctcggcaaca gattgataga acctgcgata tggcctttac
    13621 ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg
    13681 tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa
    13741 gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt
    13801 gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa
    13861 ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc
    13921 ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt
    13981 ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc
    14041 ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg
    14101 atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg
    14161 taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag
    14221 tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg
    14281 gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct
    14341 ttgctaatcc agtgctggct ggcgcggtac ataaagcgaa aaacgcaggt aaagcagtac
    14401 acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga
    14461 tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg
    14521 gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg
    14581 ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg
    14641 ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt
    14701 tccaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg
    14761 agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg
    14821 gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat
    14881 tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga
    14941 tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg
    15001 ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa
    15061 ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag
    15121 gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa
    15181 tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca
    15241 ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg
    15301 cggttaaagc ggttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt
    15361 ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc
    15421 cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg
    15481 gtgataagaa cgtgaaagcg gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt
    15541 tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg
    15601 aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc
    15661 gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt
    15721 gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg
    15781 ccgcaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat
    15841 tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca
    15901 cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc
    15961 agcaaaaagc cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg
    16021 gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt
    16081 tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc
    16141 aaacgcgtga agaagtcacc atgcagcatg ctgaactgga agagaaacaa agcgagcaac
    16201 aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgagc
    16261 gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg
    16321 aactgcgtgc caacgaatcc cgtctgcgta acagcattgc ccgtgctgaa gccgcggcga
    16381 aagcgcgtgc tgaacgagaa gcgcgtgagg cccaggcggt tcgcgagcgc cagaaagaag
    16441 cgacgcgcaa aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa
    16501 ctggtggcct gggtgcgccg cgtggtcagg cattctggcc ggttcgcggg ccgacgctgc
    16561 atcgctatgg cgagcagcta cagggtgaat tacgctggaa agggatggtt atcggtgcct
    16621 ctgaaggtac tgaagttaaa gcaattgccg acggtcgagt gattctggct gactggctgc
    16681 aaggttacgg tctggtggtg gtggttgagc acggtaaagg cgacatgagt ctttacggct
    16741 ataatcagag cgcactggtg agcgttggtt cgcaggttcg cgcgggccag ccaattgcac
    16801 tggtgggcag cagtggcggt cagggtcggc cttcactcta tttcgaaatt cgccgccagg
    16861 gtcaggcggt caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta
    16921 acgttcttgc atttgccgct ctgttggcgc tctcctcccc cgtacttgct ggcaaacttg
    16981 ccatcgtcat tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc
    17041 cttccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag atggcgacca
    17101 aagcgcataa cagcggtcac gaagtgttga ttcatctccc gatggcaccg ttgagtaaac
    17161 agccgctgga gaaaaatacg ctacgcccgg agatgagcag cgacgaaatt gagcgcatta
    17221 ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caacaaccac atgggcagca
    17281 agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcgctggag cgttacaatc
    17341 tttacttcct cgacagcgta accatcggta atacccaggc gatgcgcgcc gcgcaaggta
    17401 ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca
    17461 tccgtgtgca atttaatcgc gcaattgatc tggcgcgtcg caacggttca accattgcca
    17521 ttgggcatcc tcacccttca acggtacgcg tgctacaaca gatggtttat aacctgccgc
    17581 cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacttcta
    17641 caccgccgaa aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc gtgaagctgt
    17701 gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg
    17761 aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca
    17821 aacagcccga agcggcgaag tttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg
    17881 atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg
    17941 gtaaccgacc ttcacgctgc gcacattggt aatcaggcgc ttatacatac ccggaagtaa
    18001 atatctcggc aatcatccgt tggcgggtaa gaatatccgg ctctttgcg
//
LOCUS       DAJTYH010000082        17582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTYH010000082 DAJTYH010000000
VERSION     DAJTYH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            123..566
                     /locus_tag="OO609_002811"
     CDS             123..566
                     /locus_tag="OO609_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6634165.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            802..1620
                     /gene="ybhA"
                     /locus_tag="OO609_002812"
     CDS             802..1620
                     /gene="ybhA"
                     /locus_tag="OO609_002812"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6634166.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1621..2679)
                     /gene="modC"
                     /locus_tag="OO609_002813"
     CDS             complement(1621..2679)
                     /gene="modC"
                     /locus_tag="OO609_002813"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6634167.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2682..3371)
                     /gene="modB"
                     /locus_tag="OO609_002814"
     CDS             complement(2682..3371)
                     /gene="modB"
                     /locus_tag="OO609_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6634168.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3371..4144)
                     /gene="modA"
                     /locus_tag="OO609_002815"
     CDS             complement(3371..4144)
                     /gene="modA"
                     /locus_tag="OO609_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6634169.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4311..4460)
                     /gene="acrZ"
                     /locus_tag="OO609_002816"
     CDS             complement(4311..4460)
                     /gene="acrZ"
                     /locus_tag="OO609_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6634170.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4589..5377
                     /gene="modE"
                     /locus_tag="OO609_002817"
     CDS             4589..5377
                     /gene="modE"
                     /locus_tag="OO609_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6634171.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5445..6917
                     /gene="modF"
                     /locus_tag="OO609_002818"
     CDS             5445..6917
                     /gene="modF"
                     /locus_tag="OO609_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6634172.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7178..8194
                     /gene="galE"
                     /locus_tag="OO609_002819"
     CDS             7178..8194
                     /gene="galE"
                     /locus_tag="OO609_002819"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6634173.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8204..9250
                     /gene="galT"
                     /locus_tag="OO609_002820"
     CDS             8204..9250
                     /gene="galT"
                     /locus_tag="OO609_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6634174.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9254..10402
                     /gene="galK"
                     /locus_tag="OO609_002821"
     CDS             9254..10402
                     /gene="galK"
                     /locus_tag="OO609_002821"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6634175.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10396..11436
                     /gene="galM"
                     /locus_tag="OO609_002822"
     CDS             10396..11436
                     /gene="galM"
                     /locus_tag="OO609_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6634176.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11639..12391
                     /gene="gpmA"
                     /locus_tag="OO609_002823"
     CDS             11639..12391
                     /gene="gpmA"
                     /locus_tag="OO609_002823"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6634177.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12558..13610)
                     /gene="aroG"
                     /locus_tag="OO609_002824"
     CDS             complement(12558..13610)
                     /gene="aroG"
                     /locus_tag="OO609_002824"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6634178.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13926..14306
                     /locus_tag="OO609_002825"
     CDS             13926..14306
                     /locus_tag="OO609_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6634179.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14420..15361
                     /gene="zitB"
                     /locus_tag="OO609_002826"
     CDS             14420..15361
                     /gene="zitB"
                     /locus_tag="OO609_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6634180.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15358..16077)
                     /gene="pnuC"
                     /locus_tag="OO609_002827"
     CDS             complement(15358..16077)
                     /gene="pnuC"
                     /locus_tag="OO609_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6634181.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16115..17158)
                     /gene="nadA"
                     /locus_tag="OO609_002828"
     CDS             complement(16115..17158)
                     /gene="nadA"
                     /locus_tag="OO609_002828"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6634182.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17407..17482)
                     /locus_tag="OO609_002829"
     tRNA            complement(17407..17482)
                     /locus_tag="OO609_002829"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17447..17449),aa:Lys,seq:ttt)
     gene            complement(17486..17561)
                     /locus_tag="OO609_002830"
     tRNA            complement(17486..17561)
                     /locus_tag="OO609_002830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17526..17528),aa:Val,seq:tac)
BASE COUNT         4482 a         4591 c         4370 g         4139 t
ORIGIN      
        1 aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt cgactgttaa
       61 ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg ttcggccttt
      121 gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct atgagatggg
      181 aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca aaaattcgca
      241 tttctctccc caccacgatt tccagattga atttaacact tgccgatgta ctggataact
      301 tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc aggacaattg
      361 cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt gacccaccgc
      421 catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa ggcagtgatt
      481 tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac cgtgacggac
      541 gagggaggga atggaaagac atttagaatt aatttcgaat gatttcaaaa tgccacttgt
      601 aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa tgctcctttg
      661 catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa tgctgaatta
      721 aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt
      781 aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt agacggcacc
      841 ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc ccgcgctcgc
      901 gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat tcatcctttt
      961 tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta tttgtatgat
     1021 tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc cctgcaactc
     1081 attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga tgcaatggtc
     1141 tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac cctgccgccg
     1201 gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca acaagttaac
     1261 gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca ttttggtaag
     1321 catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca ggttgatatt
     1381 gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc gcaaggttgg
     1441 tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat gctggaagcc
     1501 gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg cgccaacatt
     1561 gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca cctgatttaa
     1621 tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag gtttgatcgc
     1681 cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc cgacttccag
     1741 ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc gcaatacgtt
     1801 acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag cctgaatgcg
     1861 gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat gctgatcgcc
     1921 cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca ctttcagaat
     1981 gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc acacttcctc
     2041 cagagcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt ctgccagatg
     2101 gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt cccgtgtcag
     2161 ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg ccagcggttc
     2221 atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca cgcgctgttt
     2281 ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc ctaaaagcgc
     2341 caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca gattgccacg
     2401 cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac gacgcttttc
     2461 cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga ggacaatccg
     2521 ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt ttccggcacc
     2581 ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa tggtcaggca
     2641 atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc cgcccgttca
     2701 cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag cgcaatagaa
     2761 ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag ggtatacatg
     2821 gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat ggttgcacca
     2881 aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc cggtaaggtc
     2941 agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt tcttgcggcc
     3001 tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac catcagcgga
     3061 aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa ggtaatacca
     3121 aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac taataataag
     3181 taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc gagcagagct
     3241 ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa cggcaggcta
     3301 aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca ttctggatcg
     3361 gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct gcctgcggtc
     3421 ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc acaacagcaa
     3481 ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact tttacccctt
     3541 tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta cgttcgacca
     3601 gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc gtatcccatg
     3661 cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt tccggatcgc
     3721 caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg tcgatggtga
     3781 aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg agcagtgtct
     3841 gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat ttctgatcgg
     3901 cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga gtagatgacg
     3961 aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta gcaatgtcct
     4021 gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca tctgccagtg
     4081 cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac catttacgag
     4141 ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact aactcggaat
     4201 tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc ggcaggaaaa
     4261 gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa tcaatgattt
     4321 tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc accaagaccg
     4381 taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc gaataccaga
     4441 ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat tctaaccgct
     4501 aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc gttttccgtc
     4561 acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca cccttaagct
     4621 ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca ttgcgctttc
     4681 cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg cctgggatgc
     4741 cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa caggcggtaa
     4801 aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc tctatgactt
     4861 actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg ccctgccgct
     4921 gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc gtaaccagtg
     4981 gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg atgtcttact
     5041 ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg cacgtctggg
     5101 gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag gcattactca
     5161 ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta gtcatattga
     5221 gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa cactgtgcgc
     5281 cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca cggcctactt
     5341 taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat ttgttatgaa
     5401 acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca tcgttgcaaa
     5461 ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct cagctaacgt
     5521 taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag tcggcgctgg
     5581 cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc cagttttccc
     5641 acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa tggcaacgga
     5701 ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg gctgagatta
     5761 ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag ttcggtatta
     5821 ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga aaaaccctgc
     5881 tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg ttcgatggcc
     5941 tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat cagtccggta
     6001 ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc cagtttgctg
     6061 gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg ctccagcaag
     6121 cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg ccggagccgg
     6181 atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg ctgaacaatg
     6241 gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag gtgaatccag
     6301 gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta ttaagcctga
     6361 ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc cgacgtcgcg
     6421 gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc agtagtttgc
     6481 atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc tattttgatt
     6541 cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag cagtggctgg
     6601 atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt tcctggggac
     6661 agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg cttattctcg
     6721 atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt tttgttgatg
     6781 tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct gaagatgcgc
     6841 ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat cgctatgtgc
     6901 tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta agcgcactct
     6961 ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga taaaaagcgc
     7021 aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa cggctaaatt
     7081 cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct ttgttatgct
     7141 atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg ttaccggtgg
     7201 tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc atgatgtcat
     7261 cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg agcgtttagg
     7321 cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga tgaccgagat
     7381 cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag ccgtcgccga
     7441 atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc tgcgcctgat
     7501 tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg ccaccgttta
     7561 tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac cgcaaagccc
     7621 ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa aagcccagcc
     7681 ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc cgtcgggcga
     7741 tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg cccaagttgc
     7801 tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg aagatggtac
     7861 tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg tggcgatgga
     7921 aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg taggcagcag
     7981 cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta actatcattt
     8041 tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca aagccgaccg
     8101 tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca cctggcactg
     8161 gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc aatttaatcc
     8221 cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc tggtttcacc
     8281 gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac aggtgttacc
     8341 tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag gcgataaaaa
     8401 ccccgattac accgggactt acgttttcac taatgacttt gcggccttga tgtctgacac
     8461 gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc gcggtaccag
     8521 ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga gcgttgcggc
     8581 attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa aaacgtaccc
     8641 gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc cgcatccgca
     8701 cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag accgcctgca
     8761 aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc agcgcgagct
     8821 ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg tgccttactg
     8881 ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc ggatcaccga
     8941 tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga ccagtcgtta
     9001 tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg caccatttaa
     9061 tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc tgctgcgctc
     9121 cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc agcgagacct
     9181 gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt ttcgcgaatc
     9241 cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca acgcatttgg
     9301 ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg gtgaacacac
     9361 cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg tgatcagctg
     9421 tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa atcagctcga
     9481 cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg cgaactacgt
     9541 tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg tggacatggt
     9601 gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac tggaagtcgc
     9661 ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac aaatcgcgct
     9721 taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca tggatcagct
     9781 aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct cactggggac
     9841 taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta acttcaaacg
     9901 taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg gtgcgcgttt
     9961 cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg ttgcacatga
    10021 gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg cccgcaccgt
    10081 tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt tgatggccga
    10141 gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg acactctggt
    10201 agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg gcggcggatt
    10261 tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac agcaagctgt
    10321 cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt gtaaaccatc
    10381 acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg tcagccgtac
    10441 cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga ctggggtgcg
    10501 actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc gctgctcggc
    10561 tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc tattggtcgt
    10621 tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt gacgctttcg
    10681 ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa acgtcgctgg
    10741 cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga tgatggcgat
    10801 cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga cgataaccgt
    10861 atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat gactaatcac
    10921 gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt gcagattctg
    10981 gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt gaaatctgtc
    11041 gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt tcttgccgac
    11101 gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc caaaggcgat
    11161 ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct gaaggtctac
    11221 accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac accgtcgcgg
    11281 ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt tctgccggac
    11341 agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg cgaagagtat
    11401 tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt catgagggct
    11461 ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact caatattcac
    11521 aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt gctgttgctt
    11581 cgtcacggca atataatgag aattattatc attaaaagat gatttgagga gtaagcatat
    11641 ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca aagaaaaccg
    11701 tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag caaaagcagc
    11761 aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt ctgtgctgaa
    11821 acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat ggctgcccgt
    11881 tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc tgaacaaagc
    11941 ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg gttttgcagt
    12001 gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc cgcgttacgc
    12061 gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca ttgaccgcgt
    12121 gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc gcgtaatcat
    12181 cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca tgagcgaaga
    12241 agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt tcgacgagaa
    12301 tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg cgaaagcagc
    12361 agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa tgagaaagcc
    12421 gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac attgcctgat
    12481 gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac tctgacggcg
    12541 catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag ttgacgtaac
    12601 agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc gtaggccagc
    12661 ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac catcacgcca
    12721 ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac atccatctgc
    12781 tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg agctggcaga
    12841 cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta gttaggctct
    12901 ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac aatcgccgaa
    12961 tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt aatggcatcg
    13021 atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca agaaagacca
    13081 gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc gccccagctc
    13141 atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc cgctggcaga
    13201 ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc gttgatctgg
    13261 aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt ggtacgcggc
    13321 ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc acgcagcgcc
    13381 agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca cgggccaatc
    13441 accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt tcgggcatgg
    13501 gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc gacaggagga
    13561 agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat gtctgttcca
    13621 gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc cgtcagaatg
    13681 tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga aaaacgccag
    13741 attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt tgtttgttaa
    13801 ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac caatgcctta
    13861 tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca taacaggagc
    13921 atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta agccttgcca
    13981 gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag gcaaacgccg
    14041 cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca aataacgtcg
    14101 acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat ggttcttcaa
    14161 tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc aaagatggtc
    14221 gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat gatgtcgata
    14281 ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg agagcggagt
    14341 ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt aagtgagaat
    14401 aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac acctgccaga
    14461 agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt ttatgctggt
    14521 agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg cgggtcatat
    14581 gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt cacgtcgtcc
    14641 tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg cgtttgtgaa
    14701 tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag aacgcttccg
    14761 cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg ggttgctggc
    14821 aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc tcaacgtacg
    14881 agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta ttattgccgc
    14941 gttgattatt atctggactg gctggacacc tgctgacccc attctctcga tactggtgtc
    15001 gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg aattacttga
    15061 aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc gggaaatccc
    15121 ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc cggtgatgac
    15181 gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc agatccaaca
    15241 ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat atcaaccttg
    15301 tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc atcaccatta
    15361 atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc ggctgccgtt
    15421 gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc cctgaagtgc
    15481 aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt ccacatactt
    15541 acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat cccagaacgg
    15601 gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt gcatgatcat
    15661 gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca tcagaccaat
    15721 cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc agcgaatttt
    15781 caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt aaatattcgc
    15841 ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa agaaaataat
    15901 gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct ccagactggc
    15961 aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg agagatcata
    16021 accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa aatccatctt
    16081 tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta gccgcaaaat
    16141 ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg acatgaacct
    16201 cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca ttcatggcca
    16261 tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt ggtgcttcca
    16321 gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga tcggttgcca
    16381 caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg gtggaaccga
    16441 ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt atggcagcat
    16501 ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca tgcacaatgc
    16561 aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa cgccccagat
    16621 gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca ataagttcga
    16681 cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca gaagtgttgg
    16741 cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat tcttcaacag
    16801 ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt gttttttccg
    16861 gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac aaagtagaag
    16921 cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag ccaccggttt
    16981 cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc accgcattac
    17041 gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca atgcttaacg
    17101 gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt acgctcatct
    17161 taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat tcgctgttat
    17221 cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa agtcactcga
    17281 attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac gatgtcatca
    17341 ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta gggcgctttt
    17401 ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
    17461 ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc gccgaccccc
    17521 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg ctggatacta
    17581 ca
//
LOCUS       DAJTYH010000083        17498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTYH010000083 DAJTYH010000000
VERSION     DAJTYH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..371
                     /locus_tag="OO609_002831"
     CDS             <1..371
                     /locus_tag="OO609_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6634183.1"
                     /translation="NMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLS
                     LITNDKITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            452..1126
                     /locus_tag="OO609_002832"
     CDS             452..1126
                     /locus_tag="OO609_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6634184.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1142..3604
                     /locus_tag="OO609_002833"
     CDS             1142..3604
                     /locus_tag="OO609_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6634185.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3620..4653
                     /locus_tag="OO609_002834"
                     /pseudo
     CDS             3620..4653
                     /locus_tag="OO609_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4735..5073)
                     /gene="rcnB"
                     /locus_tag="OO609_002835"
     CDS             complement(4735..5073)
                     /gene="rcnB"
                     /locus_tag="OO609_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6634186.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5292..6122)
                     /gene="rcnA"
                     /locus_tag="OO609_002836"
                     /pseudo
     CDS             complement(5292..6122)
                     /gene="rcnA"
                     /locus_tag="OO609_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6244..6516
                     /gene="rcnR"
                     /locus_tag="OO609_002837"
     CDS             6244..6516
                     /gene="rcnR"
                     /locus_tag="OO609_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6634187.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6608..6704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6739..7527
                     /gene="thiM"
                     /locus_tag="OO609_002838"
     CDS             6739..7527
                     /gene="thiM"
                     /locus_tag="OO609_002838"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6634188.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7524..8324
                     /gene="thiD"
                     /locus_tag="OO609_002839"
     CDS             7524..8324
                     /gene="thiD"
                     /locus_tag="OO609_002839"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6634189.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8389..9210
                     /locus_tag="OO609_002840"
     CDS             8389..9210
                     /locus_tag="OO609_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6634190.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTEKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9258..10004
                     /locus_tag="OO609_002841"
     CDS             9258..10004
                     /locus_tag="OO609_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6634191.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(9978..10943)
                     /locus_tag="OO609_002842"
     CDS             complement(9978..10943)
                     /locus_tag="OO609_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6634192.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(10940..11943)
                     /locus_tag="OO609_002843"
                     /pseudo
     CDS             complement(10940..11943)
                     /locus_tag="OO609_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            11983..13035
                     /gene="fbaB"
                     /locus_tag="OO609_002844"
     CDS             11983..13035
                     /gene="fbaB"
                     /locus_tag="OO609_002844"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6634193.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13343..14197
                     /gene="gatY"
                     /locus_tag="OO609_002845"
     CDS             13343..14197
                     /gene="gatY"
                     /locus_tag="OO609_002845"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6634194.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14226..15488
                     /gene="gatZ"
                     /locus_tag="OO609_002846"
     CDS             14226..15488
                     /gene="gatZ"
                     /locus_tag="OO609_002846"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6634195.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15498..15950
                     /gene="gatA"
                     /locus_tag="OO609_002847"
     CDS             15498..15950
                     /gene="gatA"
                     /locus_tag="OO609_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6634196.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15981..16265
                     /gene="gatB"
                     /locus_tag="OO609_002848"
     CDS             15981..16265
                     /gene="gatB"
                     /locus_tag="OO609_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6634197.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16269..>17498
                     /locus_tag="OO609_002849"
     CDS             16269..>17498
                     /locus_tag="OO609_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6634198.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQ"
BASE COUNT         4553 a         4022 c         4473 g         4450 t
ORIGIN      
        1 ttaatatgcc gactgtacca accactgtct ttgaaggtaa agctaaatat tctacctatg
       61 atgatgcagt cggtgtaacc agcagcatgt taaaaattag ctgcccgaaa gaagttgctg
      121 gtgtaaaact ctcgttgatc acaaacgaca aaataaccgg taacgataag gcgatagcca
      181 gtagcaacga taccgtaggt gataacagcg atgtcctaga tgtttctgca ccttttaaca
      241 ttgagagtta taaaacagcg gaaggtcaat atgctattcc gtttaaagca aaatacctga
      301 aactgacaga taactcagtg caatcaggtg atgtgttatc ttctctggtt atgcgtgtgg
      361 cgcaggatta ataaataaaa ttgcaagttt ctccaggtaa taaacatggc cgctatcccc
      421 atggcggctt tttaatttaa gaggcattaa gatgaaagga ttattatctt tactcatttt
      481 ttctatggtc cttcctgcac atgccggaat tgttatctac gggacgcgca ttatttaccc
      541 ggcagaaaat aaagaagtta tggtgcagtt gatgaaccag ggaaatcgtt cttcgctgct
      601 gcaggcgtgg attgatgatg gcgatacgtc gttaccacca gaaaaaattc aggttccttt
      661 catgttaacg ccaccagtgg caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa
      721 aattatgccg aataaactgc ccactaataa agaaagcatt ttttatctga atgttctgta
      781 tattccacca aatagtccag agcaagaagg caagaatgca ctgaagtttg cgatgcaaaa
      841 cagaattaag ttgttttacc ggccagcggg tattgctccg gtaaataaag cgacctttaa
      901 aaagttactg gtaaatcgca gtggcaatgg attggtgata aaaaatgact cagctaattg
      961 ggtgacgatt tcggatgtca aagctaataa tgttaaagtc aattatgaaa ctattatgat
     1021 tgccccctta gaaagtcaga gtgttaatat caaaagtaat aatgcaaata actggtatct
     1081 gaccattatc aatgaccacg gcaactatat tagtgacaaa atttaattgc aggagctgcc
     1141 tatgttgaga atgaccccac ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc
     1201 ttatgcagct gaagaaacct ttgataccca ttttatgata ggtggaatga aagaccagca
     1261 ggttgcaaat attcgtcttg atgataatca acccttaccg gggcagtatg acatcgatat
     1321 ttatgtcaat aagcaatggc gcgggaaata tgagattatt gttaaagaca acccgcaaga
     1381 aacatgttta tcaagagaag ttatcaagcg gttaggcatt aatagcgata acttcgccag
     1441 cggtaagcaa tgtttaacat ttgagcaact tgttcagggt gggagctata gctgggatat
     1501 cggggttttt cgtctcgatt tcagtgtccc gcaggcttgg gtggaagaac tggaaagtgg
     1561 ctatgttcca ccggaaaact gggagcgggg tattaatgcg ttttatactt cttattatgt
     1621 gagtcagtat tacagcgact ataaagcgtc gggtaacaac aagagtacat atgtacgttt
     1681 taacagcggg ttaaatttac tggagtggca actgcattct gatgccagtt tcagtaaaac
     1741 aaataacaat ccaggggtgt ggaaaagcaa taccctgtat ctggaacgtg gatttgccca
     1801 atttctcggc acgcttcgcg tgggtgatat gtacacatca agcgatattt ttgattctgt
     1861 tcgcttcagc ggtgtgcggt tgtttcgtga tatgcagatg ttgcctaact cgaaacaaaa
     1921 ttttacgcca cgggtgcagg ggattgctca gagtaacgcg ctggtaacta ttgaacagaa
     1981 tggttttgtg gtttatcaga aagaggttcc tcctggcccg tttgcgatta cagatttgca
     2041 gttggccggt ggtggagcag atcttgatgt tagcgtgaaa gaggcggacg gctcggtaac
     2101 cacctatctg gtgccttatg cagcggtgcc aaatatgctg caacccggcg tgtcgaaata
     2161 tgattttgcg gcgggggcga gcaagcaaag tgattttgtc caggcaggtt atcagtatgg
     2221 ttttaataac ttattgacgc tgtatggcgg cacgatggtt gctaataatt actatgcgtt
     2281 taccctcgga acgggttgga acacgcgcat tggcgcaatt tccgtcgatg ccacgaagtc
     2341 gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa agttatcaaa ttgcctacaa
     2401 caaatttgtg agccaaacgt cgacgcgttt tggtctggcg gcctggcgtt attcgtcgcg
     2461 tgattaccgg acatttaacg atcacgtttg ggcaaacaat aaagataatt atcgccgtga
     2521 tgaaaacgat atctatgaca ttgccgatta ttaccagaac gattttggcc gtaaaaatag
     2581 cttttctgcc aatatgagcc agtcattgcc agaaggctgg ggttctgtgt ccttaagtac
     2641 gttatggcga gattactggg ggcgtagcgg cagcagtaag gattatcagt tgagttattc
     2701 caataactgg cggcggataa gctataccct cgcggcaagc caggcgtatg gcgagaatca
     2761 tcatgaagag aaacgtttta atatttttat atcaattccc tgtgattggg gtgatgacgt
     2821 tacgacgcct cgtcggcaaa tatatatgtc taactcaacg acgtttgatg atcagggttt
     2881 tgcctcaaat aatacgggat tatcaggaac agtagggagt cgggatcagt tcaattatgg
     2941 tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca gctggggcga atttgacctg
     3001 gaacgcgccg gttgcgacag tgaatggcag ttatagtcag tcgagtactt atcgacagac
     3061 tggagccagt gtttcagggg gcattgtcgc ctggtcgggt ggcgttaatc tggcgaaccg
     3121 tctttccgaa acgtttgctg tgatgaatgc gccaggaatt aaagatgctt atgtcaatgg
     3181 gcaaaaatat cgcacaacaa accgtaatgg agtggtggta tacgacggaa tgacacctta
     3241 tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat agcgaagcag aattacgtgg
     3301 caaccggaaa attgccgccc cttatcgcgg cgcggttgta ctggttaatt ttgataccga
     3361 tcagcgcaag ccctggttta taaaagcgtt aagagcggat ggtcaaccat taacgtttgg
     3421 ttatgaagtc aatgatatcc atggtcataa tattggcgtt gtcggccagg gaagccagtt
     3481 atttattcgc accaatgaaa taccgccatc ggttaatgta gcaattgata agcaacaagg
     3541 actttcatgc acaatcacct tcggtaaaga gattgatgaa agtagaaatt atatttgcca
     3601 gtaatagtca ggtggttcta tggaaattcg cataatgcta tttatattaa tgatgatggt
     3661 tatgcctgtg agctatgcgg catgttatag tgagttatct gttcagcaca atttggttgt
     3721 tcaggggaat tttgcactta ctcaaacaca aatagcgaca tatgagcata attttaatga
     3781 ttcgtcatgc gtgagtacaa atactatcac ccctatgagc ccatcggata ttattgttgg
     3841 actttataac gataccatta aattaaattt acattttgaa tggaccaata aaaacaacat
     3901 cacgttgtca aataatcaga gcagtttcac cagtggttat tcggtaacgg tgacacctgc
     3961 ggccagtaat gcaaaagtga atatttctgc ggggggcggt ggttcagtga tgattaatgg
     4021 tgttgcgaca ttatccagtg cttcatcatc gacacgcggg agtgccgcag tacaatttct
     4081 actgtgttta ttaggtggca agtcatgggg tgcatgtgta aatagctaca gaaatgcatt
     4141 ggcacaaaat gcaggtgtct attccttcaa tctgacattg tcatacaacc cgataaccac
     4201 aacctgcaaa caggacgatt tattaattac tttagacagt attcccgttt tacaattacc
     4261 agccacaggt aacaaagcaa caataaatag taaaaaaagg gatattattc tgcgttgtaa
     4321 aaatttatta ggtcaacaaa atcaaacatc acggaaaatg caggtgtatt tatcaagttc
     4381 tgacttgtta accaacagca acacaatact gaaaggtgcg gaagataatg cgtaggattt
     4441 attcttgaaa gtaatggttc gccagtcaca cttttaaata tcactaacag cagtaaagga
     4501 tacacaaatt taaaagaaat tgcggcaaag tcaaaactta cagatacaac ggtttcaatt
     4561 ccgataacag ccagttacta cgtctacgat acaaacaaag ttaaatctgg cgcactggag
     4621 gcaaccgcat taatcaacgt gaaatacgac taataacaag caaaacgagc aagtggctaa
     4681 taataaaaaa taacaaggtg tctgcatttc cccctcatga tgagggggct ttttttagcg
     4741 atgataaaaa atctcaccgt cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa
     4801 cacgtacgcg ccacccatat aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg
     4861 taactgccac tgcttaatgt tgtactcatc ggtgcggtac atctctggga cggtatcacc
     4921 tggtttgaaa tgcttaaagt cagcaatgaa actacttaac tcatacttag aaattcccga
     4981 ggtattggtc gaacccgcgg tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag
     5041 cacacccagc aacatcttat ttttaatggt cataaactct ccactttggg gatctgtaat
     5101 atcagtcgca tatctttcct gtcatacaat ataactgaca agcgatattt tgttagtgat
     5161 cccatgattt cgtaaaaagt gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag
     5221 agagtcagcg gaaatagctg atttaagaat catcctgggc tgaagataac atgcaagcct
     5281 aaaggattga gttatcgcat tatgcccatg aagccatgta cgcccatata cacaccgact
     5341 aagccaatca acagactgga aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc
     5401 cagcgttttg cgacctgctg aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt
     5461 aacgccaggc caatgctgaa actgacgacc agtgttgcgc ccagtgtcag ggctttcaac
     5521 tgaatgcata tcaacagcac ggtaattgct gccgggcagg ggataaagcc accggttaag
     5581 ccaaataaca aaatttgcca gttgatgacc tctctaccat caaagcgtcg tttaatgtca
     5641 ttggcatggg ctcgtgcatg ggcatcctga tactcgccat gttcgtgatg gtgatgatgt
     5701 ccttggtcgt ggtgatgttc gtgatcgtga tgatggtgat gatgctcata atcatgcccg
     5761 tgcatattct ccagccagtt gcgttcgccg cgctaggtac gccagaacat ccagaacgcg
     5821 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     5881 aagcgcttac tgatcaccat cccgccaaag gcaatcaacc agaccaccgc ggtatgcgaa
     5941 atggttgctg ccagtccgag catcaccgcc tgtttaatgg tgcctttgat ggcgatgata
     6001 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     6061 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     6121 atgataataa ttcttagtat taattcggca atctgattct actccccccc agtacctgat
     6181 actacccccc cagtagatta atagtgctat gatttttcat attcttgtta accaggtgtt
     6241 gccatgtctc atacaatccg tgataaacag aaactgaaag cgcgtgccag taagattcag
     6301 ggccaggtcg tggcgctcaa gaaaatgctc gacgagccgc acgaatgcgc tgcagtttta
     6361 caacagattg ctgctatccg tggagcggta aacggtctga tgcgggaagt gattaaaggt
     6421 catctgacgg aacacatcgt tcaccagggg gatgagctaa aacgtgaaga agatctggat
     6481 gtcgttctga aggtgctgga ttcatatatc aaataattta ttaacgcgat tgtaaaactg
     6541 ccgtttttcc tcgtttacaa cgggggcgct ggacattacc atcctcctct gcgatttatc
     6601 atcgcaacca aacgactcgg ggtgcccttc tgcgtgaagg ctgagaaata cccgtatcac
     6661 ctgatctgga taatgccagc gtagggaagt cacggaccac caggtcattg cttcttcacg
     6721 ttatggcagg agcaaactat gcaagtcgac ctgctgagtt cagcgcaatc tgcgcacgcg
     6781 ttacaccttt ttcaccaaca ttcccctctt gtgcactgca tgaccaatga tgtggtgcaa
     6841 acctttaccg ccaatacctt gctggcgctc ggtgcatcgc cagcgatggt tatcgaaacc
     6901 gaagaggcca gtcagtttgc ggctatcgcc agtgccttgt tgattaacgt tggcacactg
     6961 acgcagccac gcgcacaggc gatgcgtgct gccgttgagc aagcaaaaag ctctcaaaca
     7021 cgctggacgc ttgatccagt agcggttggt gcgctcgatt atcgccgcca tttttgtcat
     7081 gaacttttat cttttaaacc ggcagcgata cgtggtaatg cttcggaaat catggcatta
     7141 gctggcattg ctaatggcgg acggggagtg gataccactg acgccgcagc taacgcgata
     7201 cccgctgcac aaacactggc acgggaaact ggcgcaatcg tcgtggtcac tggcgaggtg
     7261 gattatgtta ccgatggaca tcgtatcatt ggtattcacg gtggcgatcc gttaatgacc
     7321 aaagtggtag gaactggctg tgcattatcg gcggttgtcg ctgcctgctg tgcgttacca
     7381 ggcgatacgc tggaaaatgt cgcatctgcc tgtcactgga tgaaacaagc cggagaacgc
     7441 gcagtcgcca gaagcgaggg gccaggcagt tttgttccac atttccttga tgcgctctgg
     7501 caattgacgc aggaggtgca ggcatgaaac gaattaacgc tctgacgatt gccggtactg
     7561 atccgagtgg tggtgcgggg attcaggccg atcttaaaac cttctcggca cttggcgctt
     7621 atggttgctc agttattact gcactggtgg cgcaaaatac ccgtggcgta cagtcggtgt
     7681 atcgcattga gcctgatttt gtcgccgccc agctcgattc ggtgttcagc gatgtgcgaa
     7741 tcgataccac taaaatcggt atgttggcgg aaaccgatat tgttgaagcg gtggcagaac
     7801 ggttgcaacg ttatcagatc caaaacgtgg tactcgacac cgttatgctg gcaaaaagcg
     7861 gcgacccgct gctttcacct tcggcggttg ctacgctgcg cagtcgatta ttgccacagg
     7921 tttcattaat aacgccaaac ttgcccgaag ctgccgcctt gctcgacgcg ccacacgcgc
     7981 gcaccgaaca ggaaatgctg gaacaagggc gatcgctgtt ggcgatgggc tgtggcgcag
     8041 tgctaatgaa aggtggtcat ctggatgatg agcaaagccc ggactggctg tttacccgcg
     8101 agggtgaaca acggtttacc gcaccgcgca ttatgaccaa aaacacccac ggcactggtt
     8161 gtacactctc tgcggcgttg gctgcactac gcccgcgcca tacaaactgg gctgacaccg
     8221 tacaggaggc aaaaatctgg ctttcatcgg cgttagccca ggccgacacg ctggaagttg
     8281 gtcacggtat tggtccggtt caccacttcc acgcctggtg gtgagcaccg tactatagct
     8341 gctttggtta ttgtcagcct ttggcactga cattcaggga tgaaaccaat gcagctaaga
     8401 attaccagtc gaaaaaaact caccgctcta ttatgcgctc ttgggctaat ttccatcgtt
     8461 gcaatttatc cgcgtcagac ggtgaatttt ttctattcga cagcagttca gattacagac
     8521 tacatccact tctacggtta tcgcccggtt aaatctttcg ctattcgcat tcctgccagt
     8581 tacaccattc acggattaga tgtttcgcgc tggcaggagc gtatcgactg gcagcgggtg
     8641 gcaaaaatgc gcgacaacga tatccgctta cagtttgctt ttattaaggc tacggaaggc
     8701 gaaaagctgg tggaccccta tttttcgcgt aactggcaat taagccgcga aaatggcctg
     8761 ctgcgcgggg cgtatcatta tttctccccg tcggtatccg cttcagttca ggcgcgatta
     8821 tttctgcaaa cggtggattt ctcacaaggc gatttccctg ccgtgctgga cgtagaagaa
     8881 cggggaaaat tatcggcaaa agaattacgc aagcgggtaa gtcagtggct aaaaatggtc
     8941 gaaaaacgca cggaaaaaaa gccgattatt tactcaggag ccgtttttta tcacaccaat
     9001 ctggcgggct atttcaatga atatccgtgg tgggtggctc actattatca acgtcgtccg
     9061 gacaatgacg gcatggcctg gcgcttctgg cagcattccg accgtggaca ggtagatggc
     9121 atcaatggtc cggtggattt taatgtgttt aatggcacgg ggatgagttg caggcattcg
     9181 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     9241 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgagc
     9301 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     9361 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     9421 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     9481 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     9541 tgaaagaagc gagaggtttt tctcagcagg tggtattgcg cgggaaaaag cccgatacgc
     9601 tatgggttaa taagcgtgtc gtgaaatgtc ccgaagaagt tgcgcagcag ctggcggccg
     9661 aagcgggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     9721 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcggaattt
     9781 ctctttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     9841 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     9901 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     9961 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
    10021 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
    10081 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
    10141 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
    10201 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
    10261 tcctgccatt gcttgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
    10321 atctcagcct cttgacgatt gagtgacact aaaggtcgac aggccatgat ccgcgccagt
    10381 aatgcgtccg ggatatcgcc aatacgtggg caaaaatcga taaacggcgt cacgtcttgc
    10441 agcttttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
    10501 aaatagagca ggttgccagg cgcaacggtt aatcgtgcca accattggcg attccattga
    10561 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagtgccaga
    10621 caccagccgt tatcgctttc ggcgttatcg atcaggctga ttaacccctc tttcgccatc
    10681 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
    10741 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
    10801 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
    10861 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagt
    10921 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
    10981 tggcatagcg gttgaaatca agctgattta ccgcatccag ctccgccttt aacgcaggat
    11041 cgacagcgtt aatgccatgc aacgcgccgc aaatcgccgt cgccatagca cctatggtgt
    11101 ctgtgtcgcc gccaagattg gcgcacagga cggcgcagcg gtttgggtcg gtttgtgcca
    11161 gttcaactag cgcaatggcg cacggaacgg actcaatagt gctggtgcct gcgccaacga
    11221 cctggtaaag ctgttcgcgg gcggattcgg tgccgtcggc attacgcaca attttcagcg
    11281 caatctccag acgtgccgcc agtgaggcgc tgaacgtggt ggtgcgtttt tgctgtgcat
    11341 gtcgcgcaat tgaaggcaat gaatcaacaa tcgctgacca gcgttctccg tcaatggcac
    11401 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
    11461 ggctggacgc cagcgccaca tcatcaataa aggaatcaac atcacgcgct ggaagcaaac
    11521 aacctaatgg cgagacgcgc atcgctgcgc cgttggttac gccgttgttt tccagttcag
    11581 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    11641 ttttgttaaa ggcgtcgaaa cgcaacgccc agtcgagaat attacgccca atcagattcg
    11701 gatcgatctt gccttcacat tccagtaacg catccgccag acacaggcca tcgaggtatc
    11761 gtcggtgaat tcggcgcggt taaaataaca ggccgcgtta ttctcctttg gtccaggcag
    11821 aaaacggtca atccagccaa agtgtgcttt aacgcggctg cgtggccaaa gctcggaggg
    11881 catccccatc gcatccccta acgcctgccc ataaagagca ccgagaatac gttctgtttt
    11941 catttaactt ccccttgaat cggaatttac gggagcatag taatgacaga tattgcgcag
    12001 ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgactat tccttctgac
    12061 cagctttatc tccccggaca tgactacgta gaccgcgtga tgattgacaa taatcgcccg
    12121 ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc tggcacagga
    12181 tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc ttcatttgct
    12241 gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga agcgggctgt
    12301 aactgtgtgg catcaactta cggcgtgttg gtgtcggtat cgcggcgcta tgcgcatcgc
    12361 attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa tacctacgat
    12421 caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc ggttggtgcg
    12481 actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc tgcggccttt
    12541 gaacgcgcgc acgagctggg catggtgaca gtgctgtggg cctatttgcg aaactccgcc
    12601 tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgactggtca ggcgaaccat
    12661 ctggcggcaa ccataggtgc agatatcgtc aaacaaaaaa tggcggaaaa taacggcggc
    12721 tataaagcga ttaattacgg ttataccgac gatcgcgtgt acagcaagtt aaccagcgaa
    12781 aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg ggctgggttg
    12841 ataaactccg gcggtgctgc aggcggtgaa actgacctca gcgatgcagt gcgtactgcg
    12901 gttatcaaca aacgcgcagg cggaatgggg ctgattcttg gacgtaaggc gttcaagaaa
    12961 tcgatggctg acggcgtgaa actgattaat gccgtgcagg acgtttatct cgatagcaaa
    13021 attactattg cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg gataagacgt
    13081 ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat catgcctaca
    13141 tagcactgcc acgtatgttt acaccgcatc cggcataaaa acacgcgcac tttgctacgg
    13201 cttccctatc gggaggccgt ttttttgcct ttcactcctc gaacaatttt catattgttg
    13261 tttttgcgat cattatctcg acatttaaaa gcaaataatt tcattatatt ttgaaatcga
    13321 aagcaaacga caagatatga aaatgtacgt ggtatcgaca aagcagatgc tgaacaacgc
    13381 acagcgcggc ggttatgcgg ttccggcatt caatattcac aatctcgaaa cgatgcaagt
    13441 ggtggtggaa accgctgcca acctgcatgc gccggtcatc atcgccggaa cgcctggcac
    13501 atttactcat gctggtacag aaaatctgtt ggcgctggtc agcgcgatgg cgaagcaata
    13561 tcaccatcca ctggcaattc atctcgacca tcacacgaaa tttgacgata tcgctcagaa
    13621 ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg catttgcctt ttgcgcaaaa
    13681 tatttcacgg gtcaaagagg tggtggattt ttgccatcgc tttgatgtca gcgtcgaggc
    13741 ggagctgggg caacttggcg gccaggaaga tgatgtgcaa gtcaatgaag tcgatgcgtt
    13801 gtacaccaac cccgcacagg cgcgtgaatt tgccgaggca accgggattg attccctggc
    13861 ggtcgccatc ggcacggctc acggtatgta tgccagcgca ccggtgcttg atttttctcg
    13921 actggagaac attcgccagt gggtgaactt accgctggtg ctgcatggcg cgtcaggatt
    13981 atcgactaag gatattcagc aaaccatcaa actggggata tgcaaaatca acgttgcaac
    14041 ggagctgaaa aatgccttct tgcagtcgtt aaaaaattac ctgaccgagc accccgaagc
    14101 gaccgatccc cgggattatt tgcagtcggc taaatccgca atgcgcgatg tggtgagcaa
    14161 agtgattgcc gattgtggct gcgagggcag ggcataacgc acctgccatt taacaaggaa
    14221 aaaacatgaa aacgttaatt gcccggcata aagctggtga acatatcggc atatgttcag
    14281 tctgttctgc ccatccgttg gttatcgaag cggcgctggc atttgatcgc aacagcacgc
    14341 gcaaagtgct gattgaagca acgtcaaacc aggtcaatca atttggcggt tataccggaa
    14401 tgacaccggc agactttcgc gaatttgttt ttacgattgc cgataaagtt gggtttgcac
    14461 gcgaacgcat tattctcggc ggcgatcatc tgggaccaaa ctgctggcag caagaaaatg
    14521 cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc atatgttcgt gctggcttca
    14581 gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga taccataccg ttagcaccag
    14641 aaacggttgc ggaacgagct gctgtgcttt gctttgctgc ggaaagtgtg gcgacagatt
    14701 gccagcgtga gcaactgagc tatgtcattg gcactgaagt tccggttccg ggcggtgagg
    14761 ccagcgccat tcagtcagta cacatcaccc atgttgaaga tgccgccaat actttacgta
    14821 cgcatcaaaa ggcctttatt gcccgtgggc tgacagaggc gttaacacgt gtgattgcca
    14881 tcgtggtgca gccgggtgtg gaatttgatc acagcaatat tatccattat cagccgcagg
    14941 aagcgcaggc actggcgcaa tggatagaaa acacccgaat ggtttatgaa gcacattcta
    15001 ccgattacca gacccggacg gcttattggg aattagtccg cgatcacttt gcaatattga
    15061 aagtcggtcc cgcattaacc tttgctttac gcgaggcgat atttgcactg gcacaaattg
    15121 agcaggaact tatcgccccc gaaaatcgca gcggttgcct ggcggtaatt gaagaagtga
    15181 tgctcgacga accgcaatac tggaaaaaat attatcgcac gggttttaac gattcattac
    15241 tggatattcg ttacagcctg tcggatcgta ttcgttatta ctggccgcat agtcggatta
    15301 aaaatagcgt tgaaacgatg atggtgaatc tgcaaggcgt ggacatccca ctgggcatga
    15361 ttagtcagta tcttcccaaa caatttgaac gcattcagtc cggggaatta tcagcaatgc
    15421 cgcatcagct tattatgaat aaaatttatg atgttttgcg cgcctatcgc tacggctgtg
    15481 cggaataagg acggtatatg actaacctgt ttgttcgtag cggaatttct tttgtcgatc
    15541 gtagcgaagt tttaacctat atcggtaatg agatgctcgc gaaaggtgtg gttcatgata
    15601 cctggccaca ggcattaatt gccagagaag cagaattccc taccgggata atgcttgagc
    15661 agcacgccat tgcaataccg cattgtgagg cgattcatgc taagtcgtca gccatttatc
    15721 tgttaaggcc aacaaataaa gttcattttc agcaagcgga tgatgataac gacgtggcgg
    15781 tatcgttggt tattgcgttg attgtggaaa atccgcagca gcaattgaaa cttttacgct
    15841 gtttatttgg caagttacaa cagcccgata tcgtcgagac actaattact cttcctgaaa
    15901 ctcagttaaa ggaatacttc acaaagtatg ttttagattc agacgaataa atccctctgt
    15961 aacaataaaa aggactattt atgaaacgca agattattgt cgcttgcgga ggcgcggttg
    16021 cgacctctac gatggcggcg gaagaaatta aagagttgtg tcagagtcat aatattcctg
    16081 ttgaattaat ccagtgtcgg gttaatgaaa tagaaaccta tatggatggt gtgcatttga
    16141 tatgtaccac tgccagggtg gatcgtagtt ttggcgatat tccgttagtt cacggcatgc
    16201 cttttgtttc tggtgtcggt atcgaagcat tacaaaataa aattctgact atcttacagg
    16261 gatgacctat gttttcagaa gtcatgcgtt atattctcga cctcggccct acagtgatgc
    16321 tgccggttgt catcattatt ttttctaaaa tattaggcat gaaggcaggc gattgcttta
    16381 aagcgggtct gcatatcggg attggctttg ttggcattgg ccttgtgatt ggcttaatgc
    16441 tggattccat tggtccggcg gcgaaagcga tggcggaaaa tttcgacctg aatctgcatg
    16501 tggtcgatgt cggctggccg ggctcttcac caatgacctg ggcgtcgcaa attgcgctgg
    16561 tggcgattcc gattgcgata ttggttaacg tggcgatgtt actgacccgt atgacgcggg
    16621 tggtaaatgt tgatatctgg aatatctggc atatgacctt caccggcgcg ttgctgcacc
    16681 tggcaaccgg ttcatggatg atagggatgg cgggtgtggt aattcacgcg gcgtttgttt
    16741 ataagctcgg cgactggttt gcccgcgata cccgaaattt ctttgagctg gaaggtattg
    16801 ctattccgca cggtacgtcg gcgtatatgg ggccgattgc ggtgctggtc gatgctatca
    16861 tcgagaaaat cccaggcgtt aaccgaatta aatttagcgc cgacgatatt cagcgcaaat
    16921 ttggcccttt tggcgaacct gtcaccgtgg gttttgtgat ggggctgatt atcggcatcc
    16981 tcgcgggtta cgatgtcaaa ggtgtattgc agctggcggt aaaaacggcg gcagttatgc
    17041 tgctgatgcc acggatgatt aaacccatca tggatggttt aacgcccatc gctaagcagg
    17101 cccgtagtcg tttacaggcg aagttcggcg gtcaggagtt cctgattggc ctggatcccg
    17161 cattactgct ggggcatacg gcggtggtat cggcaagcct gatttttatt ccgctcacca
    17221 ttttaattgc tgtttgtgtt ccgggtaatc aggtactgcc gtttggcgat cttgccacta
    17281 tcggcttctt cgtggcgatg gcggtcgcgg tgcatcgtgg aaatctgttc cgcaccttaa
    17341 tctcgggtgt catcattatg agcatcaccc tgtggatcgc gacgcaaact attggtttgc
    17401 acacccaacc ggcggctaat gctggggcgt taaaagccgg gggtatggtg gcttcaatgg
    17461 atcagggcgg ttctcccatt acctggttac tgattcag
//
LOCUS       DAJTYH010000084        16989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTYH010000084 DAJTYH010000000
VERSION     DAJTYH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..376)
                     /locus_tag="OO609_002850"
     CDS             complement(<1..376)
                     /locus_tag="OO609_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Specifically methylates the ribose of guanosine
                     2251 in 23S rRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6634199.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVI"
     gene            complement(556..2997)
                     /gene="rnr"
                     /locus_tag="OO609_002851"
     CDS             complement(556..2997)
                     /gene="rnr"
                     /locus_tag="OO609_002851"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6634200.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3036..3461)
                     /gene="nsrR"
                     /locus_tag="OO609_002852"
     CDS             complement(3036..3461)
                     /gene="nsrR"
                     /locus_tag="OO609_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6634201.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3666..4964)
                     /gene="purA"
                     /locus_tag="OO609_002853"
     CDS             complement(3666..4964)
                     /gene="purA"
                     /locus_tag="OO609_002853"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6634202.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5068..5265)
                     /locus_tag="OO609_002854"
     CDS             complement(5068..5265)
                     /locus_tag="OO609_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6634203.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5347..6351)
                     /gene="hflC"
                     /locus_tag="OO609_002855"
     CDS             complement(5347..6351)
                     /gene="hflC"
                     /locus_tag="OO609_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6634204.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6354..7613)
                     /gene="hflK"
                     /locus_tag="OO609_002856"
     CDS             complement(6354..7613)
                     /gene="hflK"
                     /locus_tag="OO609_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6634205.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7699..8979)
                     /gene="hflX"
                     /locus_tag="OO609_002857"
     CDS             complement(7699..8979)
                     /gene="hflX"
                     /locus_tag="OO609_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6634206.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9055..9363)
                     /gene="hfq"
                     /locus_tag="OO609_002858"
     CDS             complement(9055..9363)
                     /gene="hfq"
                     /locus_tag="OO609_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6634207.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9449..10399)
                     /gene="miaA"
                     /locus_tag="OO609_002859"
     CDS             complement(9449..10399)
                     /gene="miaA"
                     /locus_tag="OO609_002859"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6634208.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10392..12239)
                     /gene="mutL"
                     /locus_tag="OO609_002860"
     CDS             complement(10392..12239)
                     /gene="mutL"
                     /locus_tag="OO609_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6634209.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12249..13586)
                     /gene="amiB"
                     /locus_tag="OO609_002861"
     CDS             complement(12249..13586)
                     /gene="amiB"
                     /locus_tag="OO609_002861"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6634210.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13605..14066)
                     /gene="tsaE"
                     /locus_tag="OO609_002862"
     CDS             complement(13605..14066)
                     /gene="tsaE"
                     /locus_tag="OO609_002862"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6634211.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14038..15585)
                     /gene="nnr"
                     /locus_tag="OO609_002863"
     CDS             complement(14038..15585)
                     /gene="nnr"
                     /locus_tag="OO609_002863"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6634212.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15584..16723
                     /gene="queG"
                     /locus_tag="OO609_002864"
     CDS             15584..16723
                     /gene="queG"
                     /locus_tag="OO609_002864"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6634213.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16706..16759)
                     /locus_tag="OO609_002865"
     CDS             complement(16706..16759)
                     /locus_tag="OO609_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634214.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3853 a         4841 c         4272 g         4023 t
ORIGIN      
        1 caatcaccgc atgaacgcct gcggcgtccg cactacgcag gcacgcgccg aggttgtgcg
       61 gatcggttac gccgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
      121 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
      181 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
      241 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
      301 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
      361 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
      421 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
      481 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
      541 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
      601 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
      661 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
      721 tttaccgacc tgacaacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
      781 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
      841 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata
      901 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
      961 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
     1021 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1081 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
     1141 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
     1201 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatatt taatggcgcg
     1261 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1321 tgccagaccg aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgtag
     1381 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
     1441 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
     1501 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1561 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     1621 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
     1681 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
     1741 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     1801 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     1861 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
     1921 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg aacacaggcc
     1981 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc
     2041 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
     2101 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2161 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
     2221 gcccgctttt gcttcttccg gcacttcttc tttcagcccc gcaacctgtt gctcaacagc
     2281 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2341 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc ggcgagtcgg
     2401 acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2461 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2521 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     2581 cgcttcacga cgacctttac ggtcagcacc cagcggctgc gccagcacct gatcgccatg
     2641 aatgcaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     2701 cagaaagccg tagccatcac ggtgaccaat aacggtacct ttcaccaggt cgaggcgttc
     2761 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     2821 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     2881 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     2941 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3001 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3061 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3121 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3181 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3241 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3301 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3361 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3421 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3481 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3541 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     3601 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     3661 cagaattacg cgtcgaacgg gtcgcgcaga atcatggttt cagtacgatc cggaccggta
     3721 gagatgatat cgatcggcac accggtcagc tcttcaatac gcttgatgta gttcagcgcc
     3781 gcctgcggca ggccgctacg atctttcacg ccgaaagtgg attcagacca gcccggcatg
     3841 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagtcact
     3901 tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag gccatccaga
     3961 acgtccagtt tagtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4021 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcacc gaattcgtta
     4081 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggccgggaa aggacctgca
     4141 cctacacgag ttgagtaagc tttgaggata cccagaacgt aatcaacata acgcgggccc
     4201 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4261 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt cgaacatgac gaaatcgcca
     4321 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4381 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4441 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4501 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcca ctttatcttc ataagcaggc
     4561 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     4621 tccagagcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     4681 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     4741 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     4801 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     4861 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     4921 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     4981 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5041 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5101 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5161 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5221 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5281 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5341 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5401 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5461 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5521 ggcatcgcct tcaccacgca taatgcggcc ctgacgctca gcttctgcca gcgttctggt
     5581 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     5641 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     5701 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     5761 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     5821 ttcggcaatg gcgttatctg ctgccggggt agtaacttca tcttctgtac ccgcagaacc
     5881 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatatc
     5941 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6001 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6061 gatgcgccat ttgatgtaag agtcgacgat caggtctttc ttctctttgg tcacaaagcg
     6121 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6181 cggtatcttg aaatgcagac caggctcata aaccaaaggt ttgttgtcat cgtcacgcag
     6241 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6301 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6361 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6421 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6481 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc acctttcagc
     6541 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
     6601 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     6661 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
     6721 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     6781 cgcggctgaa cttcgttgga atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     6841 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agcagcctgg
     6901 aagttgacgt ccagcagcgt gatacccatg tcatacggac gaatcgtctc ttccagttcg
     6961 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7021 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7081 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7141 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7201 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7261 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7321 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7381 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7441 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7501 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7561 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     7621 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     7681 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
     7741 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
     7801 ccactctttt tctattgcct gaagctgata aaaacgactc ctcagacgcc cttcctgcgg
     7861 tggcagacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     7921 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     7981 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8041 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8101 ttcttgtaca cgcacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8161 ctcttgtaac gtggctttaa atgccgccac cagatcgtgc ggcaggtggc gaataaaccc
     8221 tacggtatct gcaagtacgg tttcaccgac atctgcaacg tcaatacgcc gcaacgtcgg
     8281 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8341 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8401 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttccaggc gcgactgtat
     8461 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8521 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     8581 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     8641 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     8701 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     8761 catcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     8821 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     8881 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     8941 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9001 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aacagcccga aaccttattc
     9061 ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat gatggtagtt
     9121 actgctggta ccgccaccgg cgttgttact gtgatgagaa accgggcgag atgggacaac
     9181 agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa
     9241 ctgatcaaaa gactcgattt gcccttgcag cttaatacca ttcaccaaat aaatagaaac
     9301 tggaacacgt tcccgacgca gtgcgttcag gaacggatct tgtaaagatt gccccttagc
     9361 cattctctct tttccttata tgcttatttg tactttgaac ctttcgattc tgaaaaaatt
     9421 gcgcacgata cgtctcaatt gtacacattc agcctgcgat agcaccaaca acctgtaata
     9481 cttcgtcacg cgcctgttct ggtttttcac tgtcaagcca gtgaacccct tcccaaccac
     9541 gcagccaggt tatctgccgc ttcgccaact gtctcgtggc gcaaacacct cgataaacca
     9601 tttcatcgta tgagatttcg ccttcaaggt aagaccacat ctggcgataa cccacgcaac
     9661 gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt
     9721 caaaacctga agccaacatc tgatgaaaac gctgctcaat gcgttgatgg agcagttcac
     9781 ggctcgccgg ggcgatggcg aactgatgca cctgatacgg tagagcgtct cctgacgttt
     9841 gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc
     9901 tttgtggatc atttggatga atccttgctg ccgcaaccgg atctacctcc tgaagttgac
     9961 gatgcaatga ctcccaacct tgctctgccg cctgttgctc aattctggcc cgcacttccg
    10021 ggtctgccga cggtagcggc gacaaccctt ccagcaatgc cttgaaatac aacatcgtac
    10081 cgcccactaa cagtgggatc cgccccgccg cggtgatatc ggccatttcc gccagcgcat
    10141 cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc gcgaatatcc agcaatcggt
    10201 gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt cccgatatcc atccctttgt
    10261 aaataaggac agaatcaacg cttatcaact ctactggtaa aattttacgc agctcaatgg
    10321 ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg
    10381 ccttactgat atcactcatc tttcagggct tttatcgccg gatgtaaatc aacagattgt
    10441 aacagaccac ccggcggcgt tttcacaagt tgcggacata accgttccac gtccgccagc
    10501 agggttatgg cctgtgccat tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc
    10561 cactgcgcaa tattgccagg ttcgaatacg gactgcttcg ccaggtagcc tatcagttca
    10621 ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag gcacggccct gatggtcaca
    10681 tgctgtgcat ctgactggaa atcaataccc aattccgcta gggcagactg cgctttttct
    10741 aatgccgatt tttcttcgcc agaaactttt agccgcaacg gaatcagcag cggctgggcg
    10801 caaacgggcg cttcacccgg cgtcaattgt gcctgacgca gccaacgttc tgccactggc
    10861 aaggataaaa gtgaaatgtt gccatcgcgc tccagcaacg cacagtcgga atggacgata
    10921 gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg caggttcctg cggttccggc
    10981 gcttttggtt tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc
    11041 tgtttctggt agcctggctg cgcattcggc cagggggcag ccggacgact gcctgaggcg
    11101 ggcgcaggag agtagcgcgg agctaccggc tcacgagctg ccggttctgc aaagtgattg
    11161 cgccccgccg ccacgcggtt ttccgggatg gcacgcggtg cgggttgtgg ttcatcgtcc
    11221 agcggtagcg gcgtttccag ttgctgttgt agcacgctca gcacgccctg atagataaaa
    11281 tcatgcacca gacgcgactg atggaaacgc acttcgtgtt tggcggggtg tacgttgacg
    11341 tccacctgat gcgggtcgat ctccagatac aacacaaatg ccggttgctg atcggccccc
    11401 agtttgtctt cgcaggcctg gcggatcgca tgattgatca ggcgatcgcg catcatgcga
    11461 ccattcacgt agcaatactg aatttccgcc agtgcgggcg tagtgtgatt tggatcggcc
    11521 acccagccgc gcagggtgag atcgccgtgt tgccattcaa tcgccagcgc ttgttcgaga
    11581 aaagcggtgc cgcaaatcgc gcctaagcgc cgttcttttt gcccgccttc cggcactgcg
    11641 cggtactgac gcacaatttt accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc
    11701 agcgcaatgc ggcggatgat ttcatcaata tggctaaatt cggttttctc ggtgcgcagg
    11761 aatttgcgcc gcgccggggt gttgtagaac agatccagca cctccagcgt cgttcccacc
    11821 ggatgcgctg ccggtttaac cgtcacgtcc atgtcgcgtc cttcggcata ggcctgccag
    11881 gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga actgatactc
    11941 gccagcgcct caccgcgaaa gcccaggctg ataatggctt cgagatcgtc cagagaggcg
    12001 attttactgg tggcatgacg agccagcgcc agcgccagct catctttttt gataccgcag
    12061 ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc
    12121 gtcgcacctg catcgaggct gttttccact agttctttga ctaccgacgc aggtcgctcg
    12181 accacctcac ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg aattggcatc
    12241 aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc
    12301 gttgcaccct gcggcgcaga ttgcatcgga tgcgcaagga agtaattgcg caggcctttg
    12361 taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg
    12421 ttgttgctga taaaaccggt ttcgaccagt actgatggga tatccggcga gcgcagaacg
    12481 ccaaggctgg cgtgttctgg tcgacgttta tgtatttcgc caatgcgttg caactgactg
    12541 atcatactgg tcgctacatc ataccctacc cgctgggaat gaccgaactg taaatccagc
    12601 accgcctggc ttaaataggg gtcagactga ctgttcgcca gcacatcacc cgccccaccc
    12661 agcagctccg acagtttctc gtgctgctcc agccagctgg ccatttcact gttggcgcga
    12721 cggttagaga gcacccatac ggaagcgcca gtcgcactac ggttcggtgc ggcatcagcg
    12781 tgaatcgaca cgaggaaatt ggcgttttgc ttatgtgcca catcactgcg ccccatcacc
    12841 gagataaagt aatccccgtc acgggttaaa acgcctttaa acatcggatc gtcattgagc
    12901 aaagtacgca atttacgcgc gatggcgatg gtgacatttt tctcccgcgt accaccgggg
    12961 ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa tagcgatgat aattttatcg
    13021 ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct gataacaccc
    13081 gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg
    13141 acaaccgcag gcgtttcaac gcgtttcgca accacgggcg gcggaggagg cggtggcggc
    13201 acatcggcgt taatcgtaaa gacgacagtg taattgctgc cattctgccg cttcaccgct
    13261 tcggttttac cgttttcggt aagatcgacc accagccgta gcgtttgtgc atctttaggc
    13321 gttccagagc gaatcgcctt caccagatta ttgccgctga acaacaacgg cagtccctga
    13381 atcatgcccg tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca
    13441 taatcaggat cgccaataaa actcaacgtt atccgcgcct gttgattacc gttagaaacc
    13501 tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc
    13561 aaccaatttc tgatgcgata catcatcccg ccacctttca aaggttaacc ggctaaacgc
    13621 gccagcaaca attcacccgc agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc
    13681 tgataatcaa tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc
    13741 cactccacca ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc
    13801 tcctcgggat cggcaaggcg gtacaaatca aagtgataga ccattaagtt gtcgagcgta
    13861 tagggttcga ccagcgtata agtggggctt ttgacattac cctgatgacc cagagcctgt
    13921 aaaaagcccc ggctaaaggt ggttttacct gcgcctaaat cgccatacag atagattacg
    13981 gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca
    14041 gggagcggaa ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg
    14101 ctgtagcgtg gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag
    14161 tacgtcagct gccgcaccgt gcgcgacaca gcctgcacag gctgcatcat acggcgacag
    14221 tttttgccca agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgctcgc
    14281 catgcctgca tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt
    14341 tccggcacct ttcagcaccg ctacgccgcc ataacgttgt accagacgtt tggcgcaatg
    14401 taagcggtca ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgctagg
    14461 atgcggcgtg atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc
    14521 atcggcatcc cacaacatcg gtttgcgaaa attctcaact ttttgcaggg cttttttccc
    14581 ccactcttgc tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc
    14641 ggtaagagag tccatagtca gttcatgcac catcaattct ggtcgtgcag tcagcagcgg
    14701 cgcaatgttc tcactgcggg tcagtactcg gactaaacca gcaccagcac gtagcgccgc
    14761 ttcccccgtc atacgaatag cccccgccgt gccgtgatcg ccaccgataa tcaccagccg
    14821 cccgtgatcg cctttatgcg aagtcgggcg acgcggtttc agccagtgag aaagttgttc
    14881 tgccgaaaac cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga
    14941 gtcaaaatgc agttgtcctg taacatcccg cgcttttcca gtgagcaagc ctagtttcag
    15001 cgcaataaaa gtgatggtgt gatcggcgtt gatcactgcg cctggcgtag cgccggtttc
    15061 agccagcagg ccggaaggga tatcaaccgc cacaatcggc gcaggatggg aattagcgtg
    15121 gtcgattaac tggctaatgg attcgcgggg cgcttgctgc aagccggtac cgagcagcgc
    15181 atcaacaatc agatctaccg attcgggcca gacaatattc gaagcatgga tctcgccacc
    15241 cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc
    15301 ctgggccaac aacgtgacct caatgccgac cgctttggcc agtcgcgcga ccacgtagcc
    15361 atcgccgccg ttattaccat gaccgcacag caccagccag tggcgggcgt caggatacgc
    15421 cgaacgacac acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag
    15481 ccccagcaca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt accagacggt
    15541 gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga
    15601 tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt
    15661 aggtattacc gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa
    15721 acaataccac ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca
    15781 tgagttatta cccggtacgc tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa
    15841 cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct
    15901 gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg agatgattca
    15961 gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg
    16021 cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg
    16081 cgaggccggt tcgttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga
    16141 tcaaccagtc gaggaaggat gcggcaaatg cgtggcctgt atgacgattt gcccgaccgg
    16201 tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga
    16261 acttgaaggg gcgctcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg
    16321 cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta cagaagacga
    16381 tttcagcccg cgtaagccgc tacacgcacc ggaactcatt gagttatttg cctggagcga
    16441 agagaagttt ttaaaagtca cggaaggatc ggcgattcgc cgtataggtc acctgcgctg
    16501 gctgcgtaat atcgccgtgg cattaggcaa tgccccttgg gatgaaacga ttttgacagc
    16561 gctggaaagt cgtaaaggtg agcacccact tctcgatgag cacatagcgt gggcgattgc
    16621 gcagcaaata gagagacgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg
    16681 tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg
    16741 aataaaaata aaaacgcatt gcaattcaag acgaataaaa tcgtcaagtg atcgactcga
    16801 caaaacggaa tcttgatttt taacttattt atcaataggt taaaaaaata ccattcaccg
    16861 tgcgaagttt ctttgaaatt aagattacag cctgaggctg tggataactc tgtttacaag
    16921 agtttgtcag agacaacaaa aaccagcaat aacgcatcgc gtcgctgtag atattttatt
    16981 gagagaaga
//
LOCUS       DAJTYH010000085        16886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTYH010000085 DAJTYH010000000
VERSION     DAJTYH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            343..909
                     /gene="mntP"
                     /locus_tag="OO609_002866"
     CDS             343..909
                     /gene="mntP"
                     /locus_tag="OO609_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6634215.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(906..1715)
                     /gene="rlmA"
                     /locus_tag="OO609_002867"
     CDS             complement(906..1715)
                     /gene="rlmA"
                     /locus_tag="OO609_002867"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6634216.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1881..2090)
                     /gene="cspE"
                     /locus_tag="OO609_002868"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1881..2090)
                     /gene="cspE"
                     /locus_tag="OO609_002868"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6634217.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2103..2246)
                     /locus_tag="OO609_002869"
     CDS             complement(2103..2246)
                     /locus_tag="OO609_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6634218.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2917..3204)
                     /locus_tag="OO609_002870"
     CDS             complement(2917..3204)
                     /locus_tag="OO609_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6634219.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3279..3422)
                     /gene="mgrB"
                     /locus_tag="OO609_002871"
     CDS             complement(3279..3422)
                     /gene="mgrB"
                     /locus_tag="OO609_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6634220.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3581..3820
                     /locus_tag="OO609_002872"
     CDS             3581..3820
                     /locus_tag="OO609_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634221.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(3963..4754)
                     /gene="kdgR"
                     /locus_tag="OO609_002873"
     CDS             complement(3963..4754)
                     /gene="kdgR"
                     /locus_tag="OO609_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6634222.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4931..6304
                     /locus_tag="OO609_002874"
     CDS             4931..6304
                     /locus_tag="OO609_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6634223.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAIRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6350..7231)
                     /gene="htpX"
                     /locus_tag="OO609_002875"
     CDS             complement(6350..7231)
                     /gene="htpX"
                     /locus_tag="OO609_002875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6634224.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7423..9471)
                     /gene="prc"
                     /locus_tag="OO609_002876"
     CDS             complement(7423..9471)
                     /gene="prc"
                     /locus_tag="OO609_002876"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6634225.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9491..10189)
                     /gene="proQ"
                     /locus_tag="OO609_002877"
     CDS             complement(9491..10189)
                     /gene="proQ"
                     /locus_tag="OO609_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6634226.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10286..10783)
                     /gene="msrC"
                     /locus_tag="OO609_002878"
     CDS             complement(10286..10783)
                     /gene="msrC"
                     /locus_tag="OO609_002878"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6634227.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10913..12196
                     /gene="yebS"
                     /locus_tag="OO609_002879"
     CDS             10913..12196
                     /gene="yebS"
                     /locus_tag="OO609_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6634228.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12165..14785
                     /gene="yebT"
                     /locus_tag="OO609_002880"
                     /pseudo
     CDS             12165..14785
                     /gene="yebT"
                     /locus_tag="OO609_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14865..16304
                     /gene="rsmF"
                     /locus_tag="OO609_002881"
                     /pseudo
     CDS             14865..16304
                     /gene="rsmF"
                     /locus_tag="OO609_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16422..16658
                     /locus_tag="OO609_002882"
     CDS             16422..16658
                     /locus_tag="OO609_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6634229.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16763..>16886
                     /locus_tag="OO609_002883"
     CDS             16763..>16886
                     /locus_tag="OO609_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR6634230.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHG"
BASE COUNT         4083 a         4271 c         4088 g         4444 t
ORIGIN      
        1 ttattttaac gtttcattga tatataaaca taaatgaaaa tcgttatcaa taaagcaatg
       61 aaataatata ttccaacagt tgttttatat tctcaaaata tgttaaggtt gcgccctcat
      121 ttggggagta gccgatttcc agattccgga aatgtacgtg tcaacatact cgttgcaaaa
      181 cgtggcacgt acggactgaa gactttcagt caggcgagac catatgcaca tcaatcgcta
      241 tgcctgcatg aacgcgaaaa cccatcgcgg cttgttgttc atgtatgcat gtttgctggg
      301 ggcgatgatg tgttttatgg ataccccggt caggacgttg tcatgaatat cactgctact
      361 gttcttcttg cgtttggtat gtcgatggat gcatttgctg catcaatcgg taaaggtgcc
      421 cccctccata aaccgaaatt ttctgaagca ttgcgaaccg gccttatttt tggtgccgtc
      481 gaaaccctga cgccgctgat cggctgggga atgggcatgt tagccagccg gtttgtcctt
      541 gaatggaatc actggattgc gtttgtgctg ctgatattcc tcggcgggcg aatgattatt
      601 gagggttttc gtggcgcaga tgatgaagat gaagagccgc gccgtcggca cggtttctgg
      661 ctactggtaa ccaccgcgat tgccaccagc ctggatgcca tggctgtggg tgttggtctt
      721 gctttccttc aggtcaacat tatcgcgacc gcattggcca ttggttgtgc aaccttgatt
      781 atgtcgacat tagggatgat ggttggtcgc tttatcggct caattattgg gaaaaaagcg
      841 gaaattctcg gcgggctggt gctgatcggc atcggcgtcc agatcctctg gacgcacttc
      901 cacggttaat aagaacgctg ccagaggtga atattaaagt ccgtctggca gtcgaacact
      961 tcttttgctg ccagtgtttg ccagacttct ggcttcgcac gccaggcaaa cggcgtcatc
     1021 tgcaataatg cgacggcttc atcaccgcga agacgcatcg gataacacaa ctccgcactc
     1081 tgctgtaatg taaaaccttc cagttgttct gcatgaggtg catgaagatg tacttcattg
     1141 taaatcagcc ccttcagctc catcaaatgt cgcggtcccg gcgtggcagt aatgacccag
     1201 ccgccgggct tcactactcg tgctaattct tctgctttac acggcgcgta aatacgtatt
     1261 atggcgtcca tactggtatc ggaaaacggc aaacggtggc tggaagcgac acaaagagtg
     1321 acctgcggat agcgtttcgc cgccgctttt atcgctacct tcgagacatc caggccaaac
     1381 gtggtgattt cgggcaacgc atcggcaaat gcgtgtgtgt aatacccttc accacagcca
     1441 atatccagca ccgccgtggc cttttcatca agccgttccc gcagttgggc gacaattgca
     1501 tcacgcagcg gctgataatg tccggcatct aagaatgcgc ggcgtgcttg catcatttcc
     1561 gcgctgtcgc ccggatcacg agaccgttta tgctgaacgg gcagcagatt gacataccct
     1621 tctttcgcca tatcaaactg atgtcgttgg ggacagatat agctgttttt ttcacgcgaa
     1681 agaggctgat ggcaaagtgg acaggaaaac gacatgacaa ctccggcagg atactaaagg
     1741 ccgcaagtgt aacgcgaatc acgcggcggg caaatatccg aacgccagga gcgaagataa
     1801 agcatagtaa aaagcctcgc attcgacgag gctttatagc gagattgaag cgtattcaca
     1861 cttcagatca gtggattcga tcagatagct gttacgttaa cagctgccgg acctttctgg
     1921 ccgtcctgaa tttcgaactc aacgttctga ccttcagcca gagttttgaa gccattaccc
     1981 tggatagcgg agaagtgtac gaacacatct ttgctgccat cagccggagt aatgaagcca
     2041 aaacctttag actcgttgaa ccacttaacc tgacctttaa tctttgccat ttgaaaaatt
     2101 ccttagattg ttttcttcgc ccgcaggcat aacatagata aaactgacac attactgcat
     2161 gaggcaccaa tataaggctc ggcagagaag cggtattcaa cgtcaacgtg tttactcagg
     2221 acttctttac tgaaaatgcc acacataaac agaactgtac ctcgtttaac ccgaaatctt
     2281 ttatcacata caacgttaat tatggcaagc catttttaaa catatctcga tcagacgcac
     2341 aaatctcgat acgtcttcca ctttttttgc acacttatgc aacggaatac gcgccgatat
     2401 atcattgtgc ttaaccttgc cagttcaggc agatacttaa actggcgtag tttctaacat
     2461 agttcaataa gttcagttgt tccaggccag agggagaagt tattacatag tgcgtgcaat
     2521 atcacatttt ctgctatgca atgaataaaa agttatatca ctttttctca taaaacagtc
     2581 agttaacggc tatcaattac cctaaagaga agtcaatccc caaagggatt gtaaatttaa
     2641 aataagaaaa attgatgagt gagcaaaaaa aatcaagaga gaaacgtttc gctaataaat
     2701 aataccacgc ttaatcacaa atctgatgta aacatcctaa taaattaatg aggaaaatat
     2761 ctttttctga tatcccgtta cgcaaataaa taatatgata attatttgaa gtcagaccag
     2821 agttttaatt tgaagcagtt gcaccatgac agtgcgtata atcaggatga attgagtaac
     2881 ccttcaacga cggggttact caagattgag gaaggattat cgttcggcga ccaggcgaat
     2941 aatatcttcg tcatcaaccg atttttgact ctcaatggtc tttttatctg ctttctctgg
     3001 ttcgtttgct tcgcacaaac gacgtaacag tgcattttga cgtttttgct gatccagcaa
     3061 cgcctcaagc agttcaatct gttcgttagt ccgtgaactg gcacgattga taaaaaacca
     3121 caagatgagc ccgataagaa gaaccaccac cgatacaacc aaagacgcaa tgttcatcac
     3181 gcctgaattt acaacttcgt tcatttcacc acctctgagt agaggcgcta ttctaccact
     3241 gctggagagg aagaaaatct agtgctgaaa aaatgatatc accacgggat aaactggtta
     3301 atggcacaaa ttccgctgaa aaattgtaca tcctgatcgc acatcatgtt gaatacctgc
     3361 gcccaaagca gcaagcaagc caacaccacg acaaccagaa cgacccatcg aaactttttc
     3421 actccaatct ccgcttcacc taccttatgt cattaaacta gcatgcgtta gttaaacagc
     3481 gcctaactat gtcgatattc gtgctttttc ggaatgagta gcttgcttca gcgcatattg
     3541 ccgctacgat taagcgaaca taaaaagaga agagattgta atgcgattca tcattcgcac
     3601 agttatgctg atagcactgg tatggatagg attattactc agcggatatg gcgttttgat
     3661 tggaagcaaa gagaatgccg caggattagg tttgcaatgt acctatctga ctgccagagg
     3721 caccagcacg gtacaatact tgcacactaa aagtggtttc ttggagataa cagattgccc
     3781 actgttacgt aaaagcaata tcgtggtcga taatggttaa gggtaataaa taaccagatt
     3841 ctcactcatg agtaaaatat tcttatagca agcgcgttta atataacgca ccttcaactt
     3901 tatttttctc atgctaaata aagaacctgt ctgtttgata ttccgacagg ttcttactct
     3961 catcagaacg gatagtcgtg ataacccatt tgggcagaaa ttttgcgcgc tgcggtatgc
     4021 aacattgcga catattcctg taaacgctct tcagagaaac gcaacgtcgg gaaggagatg
     4081 ctcaaaccgg caatgaccac gccaaagcga tcaaataccg gtaccgcaat gcattgcagc
     4141 ccttcttcct gctcttcgtt atcttctcca tacccctgct cgcgcacctg gtccagaacg
     4201 ggtaataacg cttctgtact ggtgatggtc cgctcggtac tgcgtttata ctccacgccc
     4261 tcaagaattt gcttcacttc atcgcgatcg cgccatgcca gcagtacctt accaatcgcg
     4321 gtgctgtaca gcggattacg acgcccaatc cgtgaataca tgcgcaaatt gtacatagag
     4381 tcaattttgt gaatgtaaac aatactgtct tcgtccagtg cgccgaggtg gatagtttct
     4441 ttggtcaggc gggagatctc acgcatctgg atatctgcgc tacgaattaa atcgacgttt
     4501 tgtaacgcgc gagcgcccag ttcaaacaat ttcagggtca gggaatattt ctccgactcc
     4561 ccttcctgcg ccacataacc taaggttttc atggtctgta aaaagcgata aacggtgctt
     4621 tttgacatca tgacgcgctg cgacagctcg gttatcccta tttcgcgctc ttcacccagc
     4681 gcctgcaaaa tgccaaaaac ttttagcacg gaagatacag aatcaggctg tttatccaga
     4741 tctgcgttag ccatttatca cctcattgcg agtgttttat aaaaatcaga actgtttttt
     4801 attataattt cgcaccaggg tggtcgcaat ccatcttttg ccggttagtt acaattctgc
     4861 gacatccacc gtgaatatca gtgctagaat catacccctg ttgattattc accaaagata
     4921 taaaattcct atgccaaaag ttcaggccga cggcctgcca ttgccccagc gatacggtgc
     4981 gatattaacc attgtgattg gtatttcgat ggctgtcctt gacggcgcaa tcgccaacgt
     5041 cgccctgcca acaattgcca cggaccttca tgccacacca gccagttcca tctgggtagt
     5101 gaacgcctat caaatcgcca ttgtcatctc cctgctctcg ttttcgtttc tgggcgatat
     5161 gtttggctat cgacgtattt ataaatgcgg tctggtcgtt tttctgttgt cttcactgtt
     5221 ctgcgccctt tctgattcgc tgcaaatgct cacccttgcg cgtgtcatac aaggtttcgg
     5281 cggtgcagcg ttgatgagcg ttaataccgc acttatccgc ctgatctatc cacaacgttt
     5341 tctggggaga gggatgggca taaactcgtt tattgttgcc gtctcttctg ctgccgggcc
     5401 gacaattgct gcagcaatcc tctccatcgc atcctggaaa tggttatttt taatcaacgt
     5461 accgttaggt attatcgccc tgcttctggc gatacgtttt ctgccaccca atggttctcg
     5521 cgccagtaaa ccccgtttcg acctgcccag cgccgtgatg aacgcgttaa ccttcggcct
     5581 gcttatcact gcgttgagtg gtttcgctca ggggcaatcg ctgacgttaa ttgctgcgga
     5641 actggtggta atggttgttg ttggtatttt ctttattcgc cgccagcttt ctcttcccgt
     5701 accgctgcta ccggtggatt tactgcgtat cccgctgttt tcactttcta tttgcacatc
     5761 tgtttgctct ttctgcgcac aaatgctggc aatggtttcc ctgccctttt acctgcaaac
     5821 cgtgctcggg cgtagtgaag tcgaaacagg tttacttctg acaccgtggc cgttagcaac
     5881 gatggtgatg gctcctctgg caggctattt gattgaacgc gtacatgcag gattgctggg
     5941 tgctttaggg ttgttcatca tggctacggg gcttttttcc ctggttctgc tgcccgcgtc
     6001 acctgcggat atcaatatta tctggccgat gatcttatgt ggcgctggat ttggcttatt
     6061 ccagtcaccc aataaccaca ccattattac ctccgctccg cgcgaacgta gcggtggggc
     6121 cagtggcatg ttaggaacgg ctcgtctact gggtcagagt agtggcgcgg cgctggtggc
     6181 gctgatgcta aatcagtttg gtgataatgg tacgcacgtc tcgctgatgg ctgcggctat
     6241 tctggcagtg attgctgcct gtgtcagtgg tttacgtatc actcagccac gagccagggc
     6301 ataaaaaaag cgcgtcgatc aggacgcgct ttttagtatt tacttcatat tacttcaggt
     6361 attcacccgt acgcagagct tcaatacgtt tatccagcgg cgggtgagtc atgaacaact
     6421 cactgagcga tttcgactta ccgttaatgc agaaagccat catgctggtt gcttcttgcg
     6481 gttcatagct ggttttcagg cgctgcagcg cggcaatcat tttctcgcga ccaaccagtt
     6541 ttgccgaacc ggcatctgca tggaattcac gatgacgcga gaaccacatg gtgataatgc
     6601 tcgccagaat accaaacacc agttccagaa ccgttgcaac cgcaaagtag atcagcgggt
     6661 tgccgttgct ctcttcacct tcatcacgat ttccgcccat aaaacccgcg gcaagctgcg
     6721 ccagaatacg ggaaataaag ataacgaagg tgttcaccac gccctgaatc agcgtcatgg
     6781 tgaccatatc accattggcg atgtggctga tttcgtgagc aattaccgcc tcggcttcat
     6841 ccgggctcat gttctgcagc aaaccggtgc tgacagcaac cagagaggca tcacggcgcg
     6901 caccggttgc aaaagcgttg atgtccggcg catggtagat agccacttgc ggcatagcga
     6961 tccccgcctg acgagcctgg gttgctacag tattgaccag ccaacgttcc ctttcgttac
     7021 gcggttgctc gatcacttcc ccgccaacag atcgtaatgc catccatttg gacatcagaa
     7081 gcgaaacgaa ggaaccacca aaaccgaaca gcaaggccat gatcatcagc ccctgaacgc
     7141 tgctcgactg tatccctgtc aggctcagta ccagcccgaa aacgaccatt acggccaggt
     7201 tcgttagcag gaagagcgcg attcgcatca taattttctt tttacctcag tttaacaaaa
     7261 cgcaatatgc gatacccaca tcgtatgggt tacgcgacta ttttcaagtc tggatagtgc
     7321 gtaagtcacc agaaagacac aactttacat tttgtagcat ctgatttacg gcatcttgcc
     7381 gctgttaaaa aatcaggcac aatttcttgt gcctgattga tattacttga cgggagcggg
     7441 ttgttccgcg ggtctggctt tttcaagctt cgccagatcg agtgcgatat tcaccgtctc
     7501 atccagataa ggatccggct cctggtaatc tttcgataga tcatccagct tcttcaactc
     7561 cggtttacct tctcgtttaa agcgttcgtt caaacgcgcc agacgcgtcg catcatcttc
     7621 attattctct ttctcacgca cagcgtagtt cagagaaacg atattgcgct tgtccttcat
     7681 agcgttgaag cgcgcgatat ccttcatgat gttctggaac tcaggatctt tcgcgatacg
     7741 cgcattatgt tccttcagca gctccggttc aaaggcagtt aaatctcctg atttcacata
     7801 agtcgcggca tcgatgctat cccacggcag cgcgttatct tcgaatttct cacccgtttc
     7861 cgtttcttca ttacccgtcg gcatgatgat gtctggtgtt acgcctttac gttgcgtact
     7921 gccgccgtta acgcgataga atttctggat cgtgtactgc acagaaccca gcgctggcca
     7981 ttcaggacgt aacatctgat cgtaaatacg gttcaatgaa cggtattgct gaacggtgcc
     8041 tttaccaaac gtcggttcac ccacaaccag cgcacgaccg taatcctgca ttgccgcggc
     8101 aaagatttct gaagccgaag cactgaaacg gtcaaccagt accaccagcg ggcctttata
     8161 gaacacctgc ccgtcggtat cgctgtcttc acgaaccttg ccgttgttat cgcggacctg
     8221 aacaatggga cccgcaggaa taaacagacc tgagagcgat accgcttcgg tcaacgcccc
     8281 accgccattg ctacgcaggt cgatgatcac actgctgaca ttctgttttt ccagtttctg
     8341 cagttgaact ttgacatcgt ctgtcaaacc cacatagaag cccggaatat ccagcacgcc
     8401 gactttctct ttaccgacgg tcttcaccga cattttaacc gcgcggtctt cgagacgaat
     8461 acgttcacgg gtcaacgtta cagtacgggt cttggtccct ttaccagcag gtaaaatttc
     8521 cagacgaact ttactgccct tcggcccttt aattaaggca accacatcat caagacgcca
     8581 gccaatcacg tcaaccatcg gcttgcctgt ttgaccaaca ccgacaattt tgtcaccaac
     8641 gctgatagct ttactcttcg ctgccggacc acctgccacc atcgaattga taacggtgta
     8701 gtcatcatcc atttgcagca ctgcgccaat accttccagt gacaaactca tttcagtgtt
     8761 gaactgttcg gtattacgcg gggaaagata gttggtatgc ggatcgattt cacgcgcaaa
     8821 cgccgtcatt gccagcgaga aaacatcttc gctgttggtt tgcgccagac gacgaatggc
     8881 aaatttgtag cggcgtgtca gggtttcacg aatttcttta tccgtttttc ctgtcagctt
     8941 caggcttaac tcgtcgaatt tgactttact gtcccactgc gcgttcaact cagcctcgtt
     9001 tttcggccag ggcgctttgc tgcggtcaag gttataagtg tcgttgccgg tgaaatccat
     9061 cggcttttcc agtaccgaca aagcgtactg gtaacgttca aaacggcgct tttgcgccag
     9121 attgtagaga tcgtagaaaa cgtcgagttt gcctgaacgc agttcatcgc ctaactcggt
     9181 tttctttttc gcgaactgtt caacatcgct tgccagcagc acattgtggc tgtaatcgag
     9241 cagattcagg tagcggtcaa agattttggc cgaaaatgcc tgatcgaggt cgaactggcg
     9301 ataatgagaa cgggtgaagc gcgacgttac gcgctcactt accgtcgcat gctgcgtctc
     9361 ttcctttaat accggaattt gatcagcacg cgtgatatct tctacagcga aggtctggcc
     9421 tgctattgca agcaggccag ctaacgcggt aagcctaaaa aacatgttca tgcctggccc
     9481 ggcctccgtt tcagaacacc aggtgttctg cgcgcacaat caaagacata cccgaattca
     9541 gctggacgcg gacgccgtct ttggtgattt ctaatacggt ggcatccatc gcgttttgac
     9601 ccgctttcac cttcagggct tgtccgacag tcagagctga aatgtcagaa accggggtgt
     9661 gctgttcttc gcgaggtgct tttactgttt ttggcgcttt ctctaccggc ttttgcgcac
     9721 gaggtttacg ttcagcgcct tctttgcggc gtggcgtggt cggacgtggc ttgcgttcgc
     9781 ggcgcggtgc gtcttctttc tcaccagcag ctgcggcagc ttcgcgtttt ttcgcttgct
     9841 gttcagcacg ctgtgcctga acacgcgctt tcgcttcttc aagctgcttg cgagcatgct
     9901 ctacatgttg ctcgtccagc tcaccgcatg ggttgccgtc aagatcgaca cgcgttgcgc
     9961 cgggtttaac accgtaaaga taacgccagc tcgaagtgta gagacgtaaa gcggatcgta
    10021 attgcgtttt gctcaggttc atttccccgg caacacgatc gaccaaatcc tgaaaaatac
    10081 caattttcag cggacgcgct tcaccttccg cactgaaaca gtggggaaaa cgttcggcca
    10141 gaaacgcgat tacttcttta ctgctattca acttaggttg attttccatg aaatttcctg
    10201 attacaacgg acgtagccaa caagcgcagg catgaacagg cgtcattata atgacgctac
    10261 cagtaaatgc tacgttatcc gttgattatc ctgcgacgct cgcaaagaat tttttgtaat
    10321 ccgtcgttgc aagcactttt tcaagctgtg ccacaagctg acgtaagcct tgctcgtcct
    10381 cgtctgtaaa gcgagcgaag acagtactat cgatgtcgag aacaccaata atctgatttt
    10441 tcaccaccag cggcagaaca atttcagaat tactcgccgc atcacaggca atatgcccgt
    10501 caaacgcgtg aacatcctcg atacgctgca cttgattgcg ggcaaccgct gtgccgcaca
    10561 ccccgcgccc gacgggtatc cggacacagg caattttgcc ctgaaatggt ccgagtacca
    10621 gtgtatcgtc ctcaagcaaa taaaaacctg cccagttaac gtcagtgaga cgctcgtata
    10681 acaacgcact ggtgttcgca agcgttgcca gaaaactggt ttctcccgcc atcagcgcgt
    10741 taaagtcgcg atttaaatcc gcgtaaaatt ctgttttgtt cattacttaa tcacttggtt
    10801 gtcttacctg gaactgccag cctattaaaa taagcattaa atgcgttaat gctcaagatc
    10861 attcccatca tgggttaaga ttaatgttaa ttcttattac atttggcacg tcatggctct
    10921 taacacacca caaattacgc cgacaaaaaa gataacagtg agggcaatcg gcgaggaact
    10981 gccgcgtggt gattaccaac gttgcccgca atgtgacatg ctgtttagcc tgcccgagat
    11041 aaattctcat caaagtgcct attgtccgcg ctgtcaggca aaaattcgcg acgggcgcga
    11101 ctggtcgcta acgcgcctgg cggcaatggc cttcaccatg ctgttgctta tgccgtttgc
    11161 ctggggcgaa ccgctgttgc atatctggct gttaggcatt cgtattgacg ccaacgtcat
    11221 gcaaggcatc tggcaaatga ccaaacaggg cgatgcgata acgggatcaa tggtcttttt
    11281 ctgcgttatt ggtgcccccc tcattctggt gtcctccata gcatatttat ggtttggtaa
    11341 ccgactggga atgaatttac gtccggtact gctgatgctt gagcgactta aagagtgggt
    11401 aatgctggat atctacctgg tcggcattgg cgttgcttct ataaaggtac aggattatgc
    11461 ccatatccag gcgggtgtcg gcttgttctc ttttgtggcg ttggtgattt taacgacggt
    11521 gacgttgtca catcttaatg ttgaggagct gtgggagcga tattatccgc agcgccccgc
    11581 tacgcgtagg gacgagaaac ttcgtgtctg tcttgggtgc cattttaccg gctatcctga
    11641 tcagcgtggt ctctgcccgc gttgccatat cccgctacgc ctgcgtcgtc gtcatagttt
    11701 gcaaaaatgc tgggcggcgc tgttagcgtc aatcgttttg ttgttaccag ccaacctatt
    11761 gcctatttct atcatttatc tgaatggtgg ccggcaggaa gatacgattc tttccggaat
    11821 tatgtcgctg gcaagtagca acattgcggt tgcgggaatc gtgtttatcg ccagtattct
    11881 ggtaccgttt actaaagtga tcgtcatgtt cactttactg ttgagcattc attttaaatg
    11941 ccagcaaggt ttacgcacac gcattctgtt actgcggatg gtgacctgga ttggacgctg
    12001 gtcgatactc gacctgtttg tcatatcttt aaccatgtcg ctgattaatc gcgatcagat
    12061 cctcgctttt actatgggac cggctgcgtt ttattttggc gctgccgtaa ttttgactat
    12121 tcttgctgtg gaatggctgg acagccgctt actttgggat gcacatgagt caggaaacgc
    12181 ccgcttcgac gactgaagcg cagattaaaa ataaacgccg tatctcacct ttctggctgc
    12241 tgcctttcat cgcgctaatg attgccggtt ggctgatttg ggacagttat caggaccggg
    12301 gtaataccgt caccatcgac tttatgtcgg cggatggtat tgttccgggc cgtacgcctg
    12361 ttcgttatca gggcgttgaa gtcggaacag tgcaggatat cagcctcagc gacgatcttc
    12421 gtaagattga agtcaaggtc agcatcaagt ccgatatgaa agatgcgctg cgcgaagaga
    12481 ctcagttctg gctgatgacg ccaaaagcat cgttggcagg tgtctccggg ctggacgccc
    12541 tcgtcggtgg gaactatatc ggcatgatgc cgggtaaagg taaagagcag gatcactttg
    12601 tcgcgctcga tacccaaccg aaatatcggc tggacaatgg cgatctgatg atccacctgc
    12661 aagcccccgc tctcggttcg ctgaacagcg gttcattggt ctatttccgc aagatcccgg
    12721 tgggaaaagt ctacgactat gccatcaatc ccaacaagca aggcgtggtg attgatgtcc
    12781 tgatcgagcg gcgttttacc gatctggtga aaaaaggtag ccgtttctga aacgtttccg
    12841 gcgttgatgc caacgtcagt atcagtggcg cgaaggtgaa actggaaagt ctggcggcac
    12901 tggttaacgg tgcgattgcc ttcgattcac ctgaagagtc gaaacctgcc gaggcggaag
    12961 atacctttgg tctgtatgaa gatctggccc acagccagcg tggagtaata ataaaactgg
    13021 aactgccgag tggagcagga ttaaccgccg actcgacgcc gttaatgtat caggggctgg
    13081 aagtcggaca gctgactaaa ctggatttaa atcctggtgg taaagtcact ggggaaatga
    13141 ccgttgatcc ctgcttcgtg aaaatacccg catcgaatta cgcaacccga aattatccct
    13201 tagcgatgct aatctcagcg ccctgctgac cggaaaaacc tttgagttgg tgcccggcga
    13261 tggcgagcca cgcaaagagt tcgttgttgt gccgggcgaa aaagccctgc tgcaagagcc
    13321 ggacgttctg acactgaccc tgacagcacc ggaaagttac ggtattgatg cgggtcagcc
    13381 gctcattctt cacggcgtgc aggtaggcca ggttatcgat cgtaaactca ccagcaaagg
    13441 cgtcaccttt accgtcgcca tcgagcctca gcatcgagaa ctggtaaaag gcgatagcaa
    13501 atttgtcgtc aacagccgtg tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg
    13561 tgccagcgcc tcagaatgga ttaacggcgg gatacgtatt ctgccgggcg ataaaggcga
    13621 gatgaaagcc agctatccac tgtatgccaa tctggaaaaa gcgctggaga acagccttag
    13681 cgatttaccc accacaacca tgagtttgag tgcagagacg ctgccggatg tgcaggcagg
    13741 atcggtagtg ctgtaccgta aatttgaagt tggtgaagtt attaccgtgc gtccgcgagc
    13801 taacgcgttc gatatcgatc tgcacattaa accggaatat cgcaaccttt tgaccagcaa
    13861 cagcgtgttc tgggcagaag gcggggcgaa agttcagctg aatggtagtg gcctgaccgt
    13921 acaggcatcc ccgctctcca gagcattaaa gggggccatt agcttcgata acctcagtgg
    13981 tgccagcgcc agtcagcgta aaggcgacaa gcgaattctg tatgcttccg aaacagcggc
    14041 ccgtgcggtt ggcaggcaga ttacgcttca cgctttcgat gccggaaaac tggcggtcgg
    14101 gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg atctgattac
    14161 cgcacgcaat gaagtacagg caaaggcggt gctctatccg gaatatgtcc agacctttgc
    14221 tcgcggtggt acgcgcttct cagtggtcac accgcaaatc tcggcagctg gcgttgagca
    14281 tctagatact atcctccagc cgtatatcaa cgtcgaacca ggccgtggca atcctcgtcg
    14341 cgactttgaa ttagaagagg ccaccattac tgattcgcgt tacctagatg gtttaagcat
    14401 tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc tgttccgtgg
    14461 tctggaagta ggtacggtta ccggaatgac gctggggaca ttgtcagatc gcgtgatgat
    14521 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
    14581 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
    14641 taaccaattt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cacttgcacc
    14701 gaaagcccaa gaaggcaaac acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
    14761 atggggaact gcgcttccca aataatgccc actgctccgg cgtgcctgcg ccggagcgct
    14821 tatgctaaac tgcgcgcctg tttttttgcc agtggtacat gctcgtggcc caacacaccg
    14881 tttatttccc ggacgcgttt ctgacacaaa tgcgcgaagc tatgccttcg acgctctcat
    14941 ttgatgattt tcttgccgcc tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc
    15001 tgaaaatctc cgttgctgat ttcctgcaat taaccgctcc ttatggctgg acgcttacgc
    15061 caattccgtg gtgtgaagaa ggtttctgga ttgaacgcga caatgaagat gcattgccat
    15121 tgggtagtac caccgagcat ttaagtggcc tgttttatat tcaggaagcc agttcaatgt
    15181 tgcccgtcgc cgccttgttt gctgacggta atgcaccaca gcgggtgatg gatgtcgctg
    15241 ctgcgccagg ctcaaaaacg acgcaaattg ccgcgcggat gaataacgaa ggggcaatcc
    15301 ttgccaatga gttttccgcc agttgggtaa aagtgttaca tgccaatatc agccgctgtg
    15361 gcatcagtaa tgttgcgctc acacattttg gtggccgcgt gtttggtgcg gcagtgccag
    15421 aaatgtttga tgccattttg ctggacgctc cctgctccgg cgaaggcgtg gtgcgtaaag
    15481 atcccgatgc gctaaaaaac tggtcacccg aaagcaatca ggaaatcgca gctacccaac
    15541 gggaactgat tgacagcgcc tttcatgcat tacgtcctgg cggtacgctg gtttactcga
    15601 cctatacctt aaaccgggaa gaaaacgaag ccgtttgccg gtggctgaaa gagacttacc
    15661 ccgacgaagt agagttttta ccacttggcg atctcttccc tggtgcaaac aaggcgctga
    15721 ccgaagaagg ttttttgcat gttttcccac aaatttacga ctgcgaaggc ttcttcgttg
    15781 ctcgtctgcg taaaactcag gcgatccccg ccttacccgc cccaaaatac aaagtgggca
    15841 atttcccgtt cagcccggtg aaagatcgcg aagccggaca aattcgtcag gcggctgcaa
    15901 gtgttggctt aaactgggat ggaaacctgc gactctggca acgcgacaaa gaactgtggt
    15961 tgttcccggt aggcattgaa gccctgatcg gtaaagtccg attttctcgc ttagggatta
    16021 aacttgccga aacgcataac aaaggttatc gctggcagca tgaagcggtt attgcccatg
    16081 cctcccccga caatgtaaac gcttttgaac tgacaccgca ggaagcgtag gagtggtatc
    16141 gcgggcgcga tgtttacccg caagccgcgc cagtggcgga tgacgtgttg gttactttcc
    16201 agcatcagcc gattggttta gccaaacgga ttggttcgcg actgaaaaac agctacccgc
    16261 gtgaactggt gcgggacggg aaacttttta ccagtaacgc atgacagcgc acaaaaaaag
    16321 cgcacttttt gactggcaca ttcgccggcc tcaactaggc tgaaaaattg tgcgatcgga
    16381 ctggtcgtac tacaatcagc agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca
    16441 taggcgcttt tgaaatcgac gacggcgaat tacacggtga atcgccgggt gatcgaacgt
    16501 taaccattcc ttgtaaatct gaccccgatt tatgtatgca actggatgcc tgggatgctg
    16561 aaaccagtat ccctgccctg ctaaatggcg aacactctgt cctttaccgt acccgttacg
    16621 atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat
    16681 ggcgggttat ttgtcctggt tattcccccg ttgtaaaatc tctcctaaac ttaacggtac
    16741 ggcaccacac ttcggggatg aaatgttcgc gctggtactt tttgtttgct acctggatgg
    16801 cggttgtgaa gatattgttg tggatgtcta caacacggaa cagcagtgtc tttattctat
    16861 gagcgatcaa cggatccgcc atggca
//
LOCUS       DAJTYH010000086        16875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTYH010000086 DAJTYH010000000
VERSION     DAJTYH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            436..1494
                     /gene="citC"
                     /locus_tag="OO609_002884"
     CDS             436..1494
                     /gene="citC"
                     /locus_tag="OO609_002884"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6634231.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKPPA"
     gene            1509..1805
                     /gene="citD"
                     /locus_tag="OO609_002885"
     CDS             1509..1805
                     /gene="citD"
                     /locus_tag="OO609_002885"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6634232.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1802..2710
                     /gene="citE"
                     /locus_tag="OO609_002886"
     CDS             1802..2710
                     /gene="citE"
                     /locus_tag="OO609_002886"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6634233.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2721..4253
                     /gene="citF"
                     /locus_tag="OO609_002887"
     CDS             2721..4253
                     /gene="citF"
                     /locus_tag="OO609_002887"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6634234.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4257..4807
                     /gene="citX"
                     /locus_tag="OO609_002888"
                     /pseudo
     CDS             4257..4807
                     /gene="citX"
                     /locus_tag="OO609_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4782..5660
                     /gene="citG"
                     /locus_tag="OO609_002889"
     CDS             4782..5660
                     /gene="citG"
                     /locus_tag="OO609_002889"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6634235.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5711..7173
                     /gene="citT"
                     /locus_tag="OO609_002890"
                     /pseudo
     CDS             5711..7173
                     /gene="citT"
                     /locus_tag="OO609_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7287..8093
                     /gene="rna"
                     /locus_tag="OO609_002891"
     CDS             7287..8093
                     /gene="rna"
                     /locus_tag="OO609_002891"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6634236.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8322..8732
                     /gene="rnk"
                     /locus_tag="OO609_002892"
     CDS             8322..8732
                     /gene="rnk"
                     /locus_tag="OO609_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6634237.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8963..10201)
                     /locus_tag="OO609_002893"
     CDS             complement(8963..10201)
                     /locus_tag="OO609_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6634238.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10422..10850
                     /gene="uspG"
                     /locus_tag="OO609_002894"
     CDS             10422..10850
                     /gene="uspG"
                     /locus_tag="OO609_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6634239.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10971..12536)
                     /gene="ahpF"
                     /locus_tag="OO609_002895"
     CDS             complement(10971..12536)
                     /gene="ahpF"
                     /locus_tag="OO609_002895"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6634240.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12781..13344)
                     /gene="ahpC"
                     /locus_tag="OO609_002896"
     CDS             complement(12781..13344)
                     /gene="ahpC"
                     /locus_tag="OO609_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6634241.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13716..14462
                     /gene="dsbG"
                     /locus_tag="OO609_002897"
     CDS             13716..14462
                     /gene="dsbG"
                     /locus_tag="OO609_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6634242.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14672..15574
                     /locus_tag="OO609_002898"
     CDS             14672..15574
                     /locus_tag="OO609_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6634243.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15721..15924
                     /locus_tag="OO609_002899"
                     /pseudo
     CDS             15721..15924
                     /locus_tag="OO609_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(15943..16875)
                     /locus_tag="OO609_002900"
                     /pseudo
     CDS             complement(15943..16875)
                     /locus_tag="OO609_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4170 a         4379 c         4075 g         4251 t
ORIGIN      
        1 ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt
       61 ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata
      121 catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa
      181 cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa
      241 aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc ttaggatgta
      301 tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa
      361 ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc
      421 aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat
      481 aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca
      541 gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaattgcc
      601 ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg actggcgctg
      661 acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca tctgtttatt
      721 tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac gctgacctgc
      781 gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg ctatgccgaa
      841 tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat gaacgccaat
      901 ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg cgactggttg
      961 catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg gctggatctg
     1021 gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc agaatacatc
     1081 atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat taaccattgt
     1141 tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct tggtgtaact
     1201 caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa ccaggatatg
     1261 cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt tgaaattgag
     1321 cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact gctggcgaaa
     1381 aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta tttgcagaac
     1441 ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agccccccgc atgagaaaca
     1501 ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct ggggatgtga
     1561 tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat agcagcgttg
     1621 agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc cgctacaacg
     1681 tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt ttacatgcac
     1741 gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg gaggattgcc
     1801 aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca tgttgtttgt
     1861 gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg atgccctgat
     1921 gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc gcatggttta
     1981 tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg tcaacgcgct
     2041 ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg cggacgttgt
     2101 gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag agatcctgcg
     2161 tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg cgattgaatc
     2221 tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt tgatcggtat
     2281 cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc cggaaggaac
     2341 tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg gtattcaggc
     2401 gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag aagccgccca
     2461 catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga ttgatctgct
     2521 gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg tcgtagaagc
     2581 cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca agatggtgga
     2641 cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt ccggcatccg
     2701 cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga caagaacggg
     2761 tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac tcaccaaagc
     2821 aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg gaagaagcga
     2881 ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct ttccgtggcg
     2941 gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt aaaaacctga
     3001 ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac attcgccagg
     3061 gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa gagatctccc
     3121 gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg catctggtac
     3181 agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt gatgaattcg
     3241 gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat gcaatggttg
     3301 atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct tatccgcata
     3361 atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat cgcgttggcg
     3421 atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc gaactgctta
     3481 ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa ggtttctcca
     3541 tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa gacaaaatgc
     3601 gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg atggttgatc
     3661 tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac agccatgctg
     3721 cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag tacgctaact
     3781 ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc gcgctggaaa
     3841 ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg cgtggcgctt
     3901 ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc gcgccactgg
     3961 tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc ccgggctcca
     4021 gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt ccggaactag
     4081 cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg cgcgaacgtg
     4141 cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc gttgccgttg
     4201 tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa taagccatgc
     4261 acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg ctcgtcagcc
     4321 gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt ccactggtct
     4381 cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc cgaattttta
     4441 atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa attcaggagc
     4501 aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc gccccggctc
     4561 gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg cggttatggg
     4621 atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat tcactgcgcc
     4681 tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa aaacccatca
     4741 actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg cctgcaacgt
     4801 caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg ctgggctggc
     4861 gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat cgcattaact
     4921 gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg gcgattcagg
     4981 gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca ccggaagcgg
     5041 tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc cgcgccactg
     5101 cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt gcggcaattg
     5161 gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct acggcggcaa
     5221 gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga ctgacggcag
     5281 gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc gaagcaggtt
     5341 atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat caggggttag
     5401 atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac ggcgatacca
     5461 acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag gcgcaaacat
     5521 tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg cagttcgaca
     5581 gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg atccttacct
     5641 gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata ttaattttcg
     5701 gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc cactggtggt
     5761 gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg catggcatta
     5821 cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc cggcaacagc
     5881 gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc tctttgatgc
     5941 caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat ggggcctggc
     6001 tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg cattagggta
     6061 tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca tggacaaacg
     6121 cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac cgtttacacc
     6181 gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc tgccgccgtt
     6241 gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt tgatgtggat
     6301 gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg accaaacgtg
     6361 ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt gcagtggttc
     6421 ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct ttcctacgtg
     6481 ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg tgatgaacta
     6541 aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt attgctcagc
     6601 ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg tctgctggca
     6661 gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg ctataacagc
     6721 gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg cctgactcgt
     6781 tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg attctcacca
     6841 aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct gtttgccagc
     6901 ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa aggtattccg
     6961 ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat catgggctgt
     7021 ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta tgtgaaatca
     7081 aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat gttgctgttg
     7141 gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct gaataaatca
     7201 tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag atagtattca
     7261 gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc gcgttgctcg
     7321 cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca aaacagtatg
     7381 gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc cagagtcaac
     7441 acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg accaacaaaa
     7501 ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt gctgcccgtg
     7561 gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc ccgaatctac
     7621 cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca ctggaaacgg
     7681 ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg gaacgctacg
     7741 aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc ggtacgatgg
     7801 tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg gataattacg
     7861 gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg ggaaaagaga
     7921 acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact gaaattcaga
     7981 tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc ttgccacaac
     8041 ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat taatcctctg
     8101 atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt atacataatg
     8161 cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg agcaacagtc
     8221 agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct tttgggtatg
     8281 tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga ccaactatca
     8341 tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc gcctatgctg
     8401 gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg tgttcgccag
     8461 aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc aatcttagcg
     8521 atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat agcaatactc
     8581 agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt ggcgattcca
     8641 ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa ctcgaatacc
     8701 agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga taactcgctt
     8761 tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc ctggttctca
     8821 ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca tggcagaagc
     8881 attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg ctgtccttta
     8941 cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc accagacctg
     9001 aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc accttacggc
     9061 actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca aacggcatat
     9121 agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt aataattctc
     9181 ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta tcaaaggcgt
     9241 cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta acaatgccgc
     9301 cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc tccagtttca
     9361 ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg gcgtctatta
     9421 ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc ggatcgctat
     9481 cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc aagcggtagg
     9541 gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag gcgattgtca
     9601 acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg atctgcacat
     9661 tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct ttatcatctg
     9721 aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg acatattccg
     9781 cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc gcgggagcag
     9841 gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg caggcggcat
     9901 attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga attaccaccc
     9961 ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc cccataaatt
    10021 catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa agatggaggt
    10081 cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc tgttcaatgc
    10141 ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc aatgctttca
    10201 tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta tcacagcggg
    10261 aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga atatattaat
    10321 cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa tattataatc
    10381 atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag gcaatcatta
    10441 tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac gctgaattcc
    10501 tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca gccagcctga
    10561 gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa catgaagcag
    10621 aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc attaaacaac
    10681 atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa ctgggggctg
    10741 atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta ggttctaacg
    10801 cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa cagataaaag
    10861 tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat aaaaaagccg
    10921 ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc ttatgcagtt
    10981 ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc gccagtggcg
    11041 atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac gcctttgacg
    11101 ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc gactgcgcct
    11161 tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc cagttcgatg
    11221 ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt gctgccgtcg
    11281 cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag gctgcgcagt
    11341 ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag cgttacgtgc
    11401 tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc gccgataacc
    11461 gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt cacgccttta
    11521 gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc cgtcgccacg
    11581 ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg tagaccacct
    11641 tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc aacgtcgtat
    11701 tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt cggtacagaa
    11761 atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc gcccatcaga
    11821 ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc cgggccggaa
    11881 ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc acgattttcc
    11941 gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg accaaactct
    12001 ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat ttcgttctgg
    12061 aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct catcaggttc
    12121 agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt ttcgaattca
    12181 aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt cgacggatga
    12241 ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag cggggagcct
    12301 gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg cttacgcact
    12361 ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat ttcagccaac
    12421 agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa ctcaacaggc
    12481 ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc gagcatgttt
    12541 atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg gatgcagctt
    12601 gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta ggtgaatttc
    12661 cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag cttacacaag
    12721 taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga cgaaggaaat
    12781 ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt ctttccattt
    12841 agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga ttttacgcag
    12901 cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct ggatgatacc
    12961 ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac gcatgttgtc
    13021 gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga ttttagcgat
    13081 ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag atactgcgta
    13141 tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac ccagttcggt
    13201 cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc ggccttcggt
    13261 atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt taaaaggttt
    13321 aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga gatgtaaggt
    13381 aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa gttttacctt
    13441 acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat
    13501 ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc agcgcctgcc
    13561 tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa caccctcata
    13621 aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt tatgcctttt
    13681 actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata cttttactgg
    13741 ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa gcgattgaaa
    13801 aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa ggttatctcg
    13861 gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag cacgctatct
    13921 ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa
    13981 tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac tggctcctcg
    14041 acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc tgcccatatt
    14101 gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg caattaagaa
    14161 cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca attcttgcct
    14221 ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag cttaagctaa
    14281 acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat gagaaactga
    14341 tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag gaaaatacgc
    14401 tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg ggaaataaat
    14461 aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc taaaaaagca
    14521 tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa tcctgagtta
    14581 agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa gtgaatatac
    14641 catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact tgaaaaagtt
    14701 cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca ttagcaaagc
    14761 tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac gcttacgtgc
    14821 gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga cgacaacagg
    14881 gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa caattaatat
    14941 agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc tcatttcttg
    15001 ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg agattgagtg
    15061 ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc gcaaagccga
    15121 tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc cattgcatac
    15181 aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca atagcactta
    15241 tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag gagttgatga
    15301 tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc gtggttcctc
    15361 tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag tcccctatga
    15421 actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag aacatccgtt
    15481 accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt tggtgttctc
    15541 ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca gctactataa
    15601 ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt acagtgttta
    15661 ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga gcgtcttcag
    15721 atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg agagcgtatt
    15781 acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg caaagactct
    15841 ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa aatctgcgtt
    15901 ttgtttatca actgggaggc gcaatgaatc gccacggata atctagacac ttccgagccg
    15961 ttgataatac tggttttcat attctgtcgg tgacatctgt tcgctagaac catgccgacg
    16021 cttactgtta taaaacattt cgatgtaatc aaaaatatca ctgcgggctt cttcccgcgt
    16081 tccgtagatc tttttcttta tccgttcacg tttcaacaac tggaaaaaac tttctgcaac
    16141 cgcattatca tggcagttac cgcgacggct catgctaccc tccaggccgt gtgatttcag
    16201 gaacgactgc cactcatggc ttgtgtactg actgccctga tccgaatgaa ccagcacctg
    16261 tttttcggga ttacgccgcc atacagccat cagcagtgcg ttcaggacaa tgtcctttgt
    16321 catccgggat tgcatggacc agccgataat tttgcgtgag aacagatcaa caacaacggc
    16381 aagatacagc cagccttcgt gggtcctgat gtaggttatg tccgttaccc aacgctcatc
    16441 aggagcatcc ggattgaact gtcgctggag cctgttgggt gacacgatac tggcctcgcc
    16501 tttacgtgcc cgcgggcttc ggtatccgac ctgagccttt attccgacac gtttcatcag
    16561 tctccagact ctgtttactc cgcactgttg cccgctgtca cgcagatcca gatggatttt
    16621 gcgataacca tagacgcatc ccgattccag ccagaactgt ttaatctgtc ctgtcagtct
    16681 caggtctgcc tgatggcgtt gtgaatgcgg ctgctgaagc caggcgtaaa aaccactggg
    16741 atgaacatcc agcacccgac agagcaggcg aacaggccag caacaggagt tgtcacggat
    16801 aaaggcgtac ctcagtcgga cagctttgcg aagtacgccg cggctttttt taatatgtcc
    16861 cgttcgtcgg taacc
//
LOCUS       DAJTYH010000087        15990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTYH010000087 DAJTYH010000000
VERSION     DAJTYH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..255)
                     /locus_tag="OO609_002901"
     CDS             complement(<1..255)
                     /locus_tag="OO609_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6634244.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYE"
     gene            complement(263..613)
                     /gene="chbA"
                     /locus_tag="OO609_002902"
     CDS             complement(263..613)
                     /gene="chbA"
                     /locus_tag="OO609_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6634245.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(672..2020)
                     /gene="chbC"
                     /locus_tag="OO609_002903"
                     /pseudo
     CDS             complement(672..2020)
                     /gene="chbC"
                     /locus_tag="OO609_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2105..2425)
                     /gene="chbB"
                     /locus_tag="OO609_002904"
     CDS             complement(2105..2425)
                     /gene="chbB"
                     /locus_tag="OO609_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6634246.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2723..3061)
                     /gene="osmE"
                     /locus_tag="OO609_002905"
     CDS             complement(2723..3061)
                     /gene="osmE"
                     /locus_tag="OO609_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6634247.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3263..4090
                     /gene="nadE"
                     /locus_tag="OO609_002906"
     CDS             3263..4090
                     /gene="nadE"
                     /locus_tag="OO609_002906"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6634248.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4320..5207
                     /gene="cho"
                     /locus_tag="OO609_002907"
     CDS             4320..5207
                     /gene="cho"
                     /locus_tag="OO609_002907"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6634249.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKERHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5167..5742)
                     /gene="ves"
                     /locus_tag="OO609_002908"
     CDS             complement(5167..5742)
                     /gene="ves"
                     /locus_tag="OO609_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6634250.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(5945..6430)
                     /gene="spy"
                     /locus_tag="OO609_002909"
     CDS             complement(5945..6430)
                     /gene="spy"
                     /locus_tag="OO609_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6634251.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6760..7728)
                     /gene="astE"
                     /locus_tag="OO609_002910"
     CDS             complement(6760..7728)
                     /gene="astE"
                     /locus_tag="OO609_002910"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6634252.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7721..9054)
                     /gene="astB"
                     /locus_tag="OO609_002911"
                     /pseudo
     CDS             complement(7721..9054)
                     /gene="astB"
                     /locus_tag="OO609_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9051..10529)
                     /gene="astD"
                     /locus_tag="OO609_002912"
     CDS             complement(9051..10529)
                     /gene="astD"
                     /locus_tag="OO609_002912"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6634253.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10526..11560)
                     /gene="astA"
                     /locus_tag="OO609_002913"
     CDS             complement(10526..11560)
                     /gene="astA"
                     /locus_tag="OO609_002913"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6634254.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11557..12777)
                     /gene="astC"
                     /locus_tag="OO609_002914"
                     /pseudo
     CDS             complement(11557..12777)
                     /gene="astC"
                     /locus_tag="OO609_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13223..14029
                     /gene="xthA"
                     /locus_tag="OO609_002915"
     CDS             13223..14029
                     /gene="xthA"
                     /locus_tag="OO609_002915"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6634255.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14196..14909
                     /locus_tag="OO609_002916"
     CDS             14196..14909
                     /locus_tag="OO609_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6634256.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLLYARHK
                     HVDLSASRRSPLTHPKNEG"
     gene            14914..15591
                     /gene="ydjY"
                     /locus_tag="OO609_002917"
     CDS             14914..15591
                     /gene="ydjY"
                     /locus_tag="OO609_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6634257.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15606..>15990
                     /locus_tag="OO609_002918"
     CDS             15606..>15990
                     /locus_tag="OO609_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6634258.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKL"
BASE COUNT         3984 a         4271 c         3885 g         3850 t
ORIGIN      
        1 ctcataaaaa ctttgatggt gcgaacctaa cggaataaaa acaaaatcgc cccgttccag
       61 taacacgcgc ttaccgttaa tctcctggaa ataacgcccg gttaatacca gagtaaattc
      121 ataatagtcg tgctgatgca gtccgctgat actctcagtt ttgttataga taaacacatg
      181 gaagtttttg ccattaaaca actgctgttc tcgggcagtg gcaatttccg gcgcgttaat
      241 cactggctgc atcatcgact ccttatgcct tcagtttttc atgaagctca attaattcag
      301 taatcagttc acgcgcaagc atggacgtca ttaaatgatc ctgagcgtgg accagcacca
      361 gactcacttt catcttacct tcgcccgcat cgccttcaat cagtttcgtc tgtaccagat
      421 gcgcttcatt caatgccatc cgtgactgat ccatcatggc tttcgctgcg gcaaaatcgc
      481 cctgcttcgc ctgtttcaat gcggcatacg ccaggctacg cgcttgtcca gagttgatga
      541 taagccccat caccacttct tccagctctt cagcttctgt ttgcgtatca ggaatattat
      601 cgagatccat catacatcgt tcctctctct tcttaccggc aagattaccc gtaccggcat
      661 cgattaaaat ttcaggggtt agcgatatct tcttcgctct cttctttatc aatcgcattt
      721 tgtgctttgt tagccaccac aacaaagggc agataaatta acgttgcgat gccaaggttg
      781 aagagtgcga ccagcaatgc ggcgacgcta ccgttggtgt taaagaaggc tcccagaccg
      841 gttggcatgg tccacggtgc aatattggtc accggaggaa tgatgcccat gtagtacgct
      901 gcgagggtga ttgccgccag aatcggttgt accagtacaa acgggataaa catcaccggg
      961 ttcatgataa ttggcagacc aaacagaatc ggttcgttaa tctggaagat gccggacggc
     1021 agcgccagtt ttgccacctg acgataatca gcacggcgag aggcgataaa gatagccagg
     1081 atcaggccta aagtcgcacc actgccccca aggaaaataa aggagtccag catcggcttg
     1141 gcccagatat ggaaggtctt accggctgcc agcgccgctt cgacggaacc atattgctga
     1201 taggtcgcga tattttccag cgcccacggc gtcataatgc cgttgtccag tgcggtcagc
     1261 gccagcgcgc catgaatacc gaagaaccag agcagtggaa caaagatcac ataggcccag
     1321 cccaccacgc tacccaacga tgccagtggg gttgagatgg tatccataat gatctgatgg
     1381 aagttggtgc cccaggtatt caacgcccag gcaataatcc ccatcacgga aagaataata
     1441 aagccgggaa ttaatgccga gaaagaacgc gatactgaag caggtacgct gtcgggcagt
     1501 ttaatgaccc aattgcggcg gacaataaag gtaaacattt ctgccaccac caggccaata
     1561 ataatcccgg agatgatatt cgccccacct aaccagtttg cgccaaccgc ataggcctca
     1621 ccgacactat atggggtgac ggtcataaat gctgcaacgg ataacaaccc agccgccagc
     1681 gcatcgactt tacgctcttc tgccagcgcc atgccaataa agaacggtgc cattaaagac
     1741 attattccta atgttccgtt atatacgttg ccgccaatac ctttcagacc attaagtgtt
     1801 tcaatggttg aggcgtcgag gcgaatacct aaggaataaa aaacgacccc tccccaaagc
     1861 ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc
     1921 gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag
     1981 ggaggagtac cttttcaagc gatgcaataa cattactcat agaaaaatac ccttaaaaac
     2041 cgcaatttaa atattacggt attgatttat gaaataactc tttgacggga aaatttaaaa
     2101 taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc acgcctaaac
     2161 catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac
     2221 gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca tcggcattct
     2281 gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga acttcatatt
     2341 tttctgcctg tgcgcgcatt tttgatacca gtaaagagat agacatgccc gcagaacaaa
     2401 acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc tgtcagccag
     2461 acactccgca agccttgacc tgcttccatg ctctgggtaa cttgcgaaac caacatgact
     2521 ttttttgtgg ctgaaaggag tatacggtat agacctgagc aatagtattt ctttgacctt
     2581 ataaattgtt tctccttgac ctgtggttat gaccctcttc acattcgggc aaataattcg
     2641 cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta
     2701 ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg tattcagcac
     2761 aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca aagtaggttt
     2821 ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga gcatggatca
     2881 tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt
     2941 ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca taagctgtac
     3001 aacccgccag catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca
     3061 tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt
     3121 caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttaaccacc
     3181 attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt tagctttaag gaagttttgt
     3241 cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc
     3301 aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta
     3361 cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc
     3421 cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgccagg aaactcgcaa
     3481 cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggctg acgaacaaga
     3541 ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg
     3601 cgcggtattg gctagcgagc aggcattgcg ggaagcaggc attgaactga gcgattttgt
     3661 ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac
     3721 cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat tcttcactaa
     3781 atatggtgac ggcggtacgg atattaatcc gctgtatcgt ctcaacaaac gccagggtaa
     3841 acagttactg acggcattag gttgcccgga acacctttat aagaaagccc caactgccga
     3901 tcttgaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa
     3961 tatcgacgac tatctggaag ggaaaaacct acctgaacag gtcgccagaa caatagagaa
     4021 ctggtatctg aaaaccgaac ataaacgccg tccgccaatt aacgttttcg atgatttctg
     4081 gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa gagtaatttg ttcacgccgg
     4141 atgcggcgtg aacgccttat tctacctaca aaactatgca aattcaatac attgcaggag
     4201 tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat caacccgcct
     4261 cttttttaat cgcctcccgc ctgttacact ggatagataa ccagcattcg gagtcaacag
     4321 tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc
     4381 ccgaacattt acattcattc cttaatgact tacccacccg accaggggtg tatctgtttc
     4441 atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc cgcagtcggg
     4501 tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc cggcgaatta
     4561 gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag
     4621 aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat
     4681 taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca
     4741 acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag agcatcgctg
     4801 atgaacaaaa actttgttat ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat
     4861 gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agacatgagg
     4921 aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg agtggtgtgt tggccttggc
     4981 aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa
     5041 actggctgtg gctgggggcg gttaattcgc tggaagaagc gacaacgtta attcggacac
     5101 ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact
     5161 atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa
     5221 cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacacgcc
     5281 ctggtcggta gtgagcagtt tgtcgcccag ttgccacgcc ccgttaatca caaacaccac
     5341 cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca
     5401 gacatccagg cgcgtcataa tattgaaatc catcgacatc tgccctgccg tcagtttcgc
     5461 ttttaccacc tggtccgccg taaaggcaaa aggctgtaac ggttttaagg tatggttaaa
     5521 gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat
     5581 gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg cacgccagta
     5641 aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcgtttcg
     5701 ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt
     5761 cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg
     5821 ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc
     5881 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     5941 aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct gtcagacgct
     6001 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     6061 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     6121 gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc
     6181 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgtggatct
     6241 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     6301 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg
     6361 cggcatgggc caggttagcc gcgccaagag ccagggtaga ggcaacaaac agtgcagtta
     6421 atttacgcat attctatatc cttcctttca gttatttatt acggctttct taagtagcgt
     6481 gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag caacggtaaa
     6541 acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga aaatttgatg
     6601 gagagtgatg agagaatatt acaacacgat gattttgcag agattatgaa gaactatacc
     6661 ggatgactgg tgataaataa agcaaataac caggattaat ctgtattaat ttataagaaa
     6721 gcaacttaat acccgcagaa tgatttctgc gggtaagtat tagcttattt tttcgagcat
     6781 taatcccgcg cgtaatccca acgctaccaa cggattaggg aataacacat actctacatc
     6841 atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc aatgttcctt tctcaaacgg
     6901 cataaaattc agcgtgtcac ttgccatatg catttcgaag gacggcgagt ggcgagtaat
     6961 ttgcgaaacc acccgataac ggagcggcgg tgttctcacg ataccgacac tctcaccaga
     7021 tagcagcgca gcaattgcgc tggcagttac tgcaaactgg cgaagatcgt tttgcccaaa
     7081 gggcaacgct ttgccaagtt ccaacgtaca ggccagcgcg ccaaaatgtc tgacgctgaa
     7141 atgggtaaac gtaccaccag gctcctgatg gaacaccagc gcctccagcc ccgccgcacc
     7201 cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt tgcggtaata caccgaactg
     7261 cggatgcaag gagccacgaa ttgcggtatg tagatcaagg tgccaacgca cagattcttt
     7321 gccctggtca taaaaatctt ccaggcactg ttccagttcg cgcgcccgac aggtttctcc
     7381 gctttcagca aatagctgcc aacgaccgcc aaacattcgg ttcatatcgc tatggcaata
     7441 acgtttccct tgcttcagcg caggaggatt cccgaggatc accagcaacc gccaacgtaa
     7501 cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc agcatttcca caggtgccgt
     7561 ctcattaccg tggatccccg cagaaatcac cagcgcgcct tgcggtggcg ttaatggcgt
     7621 cagttccagc acaccatcgc ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt
     7681 gataaccggt tttttacccg ttaaggtcag agcaagaaaa ttatccattg ccccctccct
     7741 cgcgctggaa cggataaacc gaaccgagat tcagtaattg gctcaataca tccagtgctt
     7801 cccgcccttc gcgcagcaat tgcgggtcgg ccagatcggc agcagtaaga cgatcgcggt
     7861 agtaacgatc cacccagtca ttgagcgcat taaacaaggt atcgttcatc atcaccgccg
     7921 gattcaccgc ccggcgttct tcttctgtca ataccacccg caaccgcagg cacgccgggc
     7981 cgccgccatt cgccatgctt tcacgtaagt caaagacttt tagttcgcta atcgggttgt
     8041 cagcggcaag gagttcattg agataacccc atactccggc gtgttcccga cactcctgag
     8101 gcagcaccaa catcatggaa ccatcatcgc ggctcaacag ttggctgtta aacagatagg
     8161 tagacaccgc atccgacacg gaaacctgag ttgccggaac ttctatcgcc ataaaaccgt
     8221 tcacccgcgt gccagtaact gtgactggcg agcgaacgcc tgttggtggc aaaacagcac
     8281 ctggcggtta ctcacggcaa tcacgtcatt atgaaaaacg ctctggtcga taacgtccgg
     8341 gttttgctgg gcgaaaatca cctgttgggg attcacctga ttaagccttg ccaccgcctc
     8401 gctggcttcg cgagtctgtc gcgccggata acgggaaggc cgggtatcat tgccttcttc
     8461 tcgcccgtag acaaaaagta gcatacccgg ttcaccgtaa tgaccgccga gacgattgtg
     8521 gtttgccgcc ccctcatcac cgagcaacgc tacctgcggc aacgccgaat ggacgctaaa
     8581 tttctcttcg tccttaaaaa tcgcttttaa cagcgattca gtgacgggcg cttccagcga
     8641 acggtgaaat ttattgttca ggttggcaac ggtgagatgc actttgccat ccagcgtatc
     8701 ggcagatggc gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg aactgacgct
     8761 ggaaagccag tgcggtgcct ggcgtgcaac tttttccagt acctgctcat cgctaccact
     8821 gaatcccaac tgacgcagca ccggaataaa cggacgctcg tgcggcggaa tcacggcctg
     8881 ggggaatccc gcatcggcaa gggctttcat tttcagtaag ccctgttttg ccgccagtcg
     8941 cgggttagac acctgaaaac ggtgacgggt agaggcttca ttaccaaacg acaggcccgc
     9001 gtaatgatgc gtcagcccta ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc
     9061 acctcatcgg aaaaatccag cccggggtta agcgtggcgg gcaatgttaa cgagtccgac
     9121 tccaggctcg ccataggcca tgcgcagtaa tctgcggcat accaggcgct ggggcgatgg
     9181 ttaccggaag caccaatgcc gccgaatggg gcggtactgg cagcaccagt aagcggtttg
     9241 ttccagttaa caatccccgc ccgcgcctcc agcaacagtt gatcgaattt ttcccgctcg
     9301 ggggaaacca gaccgcaaaa gagtccgaag cgagtgttgt tcgccatccg aatcgcctca
     9361 tcaaaagtat cataacgcca gacgcgcaaa aacggcccga acacctcttc atccggcaca
     9421 ccgccaacgc ccgtcatttc aatgatccct ggtgtcagca acgatgtccc tgcttgtaat
     9481 aagcgcggcg caagcagggt tcgtccgccc atcgcttcca gttgctgcca ggcagtaacc
     9541 acctgctgtg cggcctgttc agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc
     9601 cagttgcccg gcgttaatcg ctggctgacg gcaaccagac tagcaagaaa cgcatcgccc
     9661 tgcgctccgc ttttgagcaa taaacggcgg gcgcaggtgc agcgttgccc ggctgtgaca
     9721 aacgccgact gaatggtcag atggacagcc gcgtcgatat ccgccacctc atcgataatt
     9781 agcggattat taccgcccat ctcaagggcg agaattttct ccggctgacc ggagagctgg
     9841 cgatgcagct ggtagccggt attggcgcta ccggtaaaca gcaaaccgtc gagatcctcc
     9901 agcgcactca gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag cacgcccggc
     9961 ggcaagccag cctgctgcca taaacgcatt accgcttcgc cactccacgg tgtcagttcg
    10021 ctgggtttaa agataatggt gttacctgcc agcaatgccg gaacgatatg tccgttcggc
    10081 aaatgaccag ggaaattata cggcccaaac accgccagca cgccgtgcgg gcgatgtcgc
    10141 aggctcgccg caccgtccgg catttcacta cgctgctcgc cggtacgaac gtgatacgcc
    10201 ttaattgata tcgcgatttt attgatcatc gccgtcactt cggttgccgc ttcccagcgc
    10261 ggcttacccg tttctctggc aataatcgcg gttaattcgg ctttattgct ttccagcaat
    10321 ccggcaaagc gttcgacaac gacctgacgt tcagccaatg agagccgcgc ccagcgcgga
    10381 aacgccgcac gggctgcccg acaagcctgt tcgacctgag cggcatcggc atcattgcct
    10441 tgccataaca cctcgcccga taccggatta cgcttcacac gcgatgcgcc ctggcccgtt
    10501 atccagtcac cgttaatcca taaagtcatg ctgttttctc ctctgcgcac aggcgcacca
    10561 gacgaacgcg atcccctgcg tggcatttga gggcatccag ttgtgcggcg gttaaaatca
    10621 aacgctcggt tgccggatcg gtacgcgcca gcaccacgcg gaaatggtga taattttcat
    10681 tggcgaccag gcaggccgga aaatcgccct gcgcaggctg cccttctgcc acttccacca
    10741 gccgactttt acggatggcg cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt
    10801 cgaagatgtc gatatagttg cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag
    10861 gcgcggtttg cggatgtacc tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg
    10921 tatagatcgg atgtttcggc atcagttctg caataaatgc cttttgcccg gtgccgcaga
    10981 gaaaatcggc gcggctaaaa tccatcgaaa agaagcgttt accgaggctt tgccagaacg
    11041 gtgaatagcc gtgttcgtca atcaccccgc gcatttcagc aaccactttg tcattaaact
    11101 tgtcgcgaaa agccgccata aacataaagc gcgatttcga cagcaaatag ccgttgccct
    11161 ctttgcgcca gtccgggtcg agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt
    11221 tactaagaaa cagcgtcggc aatgcgttat agacattcag ctcttttgag gcgtgaacca
    11281 acgtgccgac gcgatagttg taccagggat cgttcagccc aaccgccacc tcaatagcgc
    11341 aaatccccgc cacggtgcct gtctcgctat cttccagcac gaacacatag ccctgctcgc
    11401 ttttgggcag ttcaccttgc caggttttga ttgccctttc gatacgcgcc gaaagcgtgg
    11461 cttcattggc gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg
    11521 ccgagacatc tgagcgctca acgggacgga tgaccatcat gatgaacctc ggctaacaaa
    11581 gtgttcgcaa gcagctgcaa agcgatccag tccggtcgtc acctcttctt cgctgacatt
    11641 gagcgcaggc gcaaaacgca ccacgttgcc acccgcaatc agtaccatca cgcctgcttt
    11701 cgccgcttcc agagagagct gtttcgcttg cccggcgtaa tcggcattca gtacacagcc
    11761 aatcaacaaa cctaagccgc gaacttcact gaacaaacca tagcggtgat taatagtatt
    11821 aagacgctca acaaaccagt cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt
    11881 gatgagctcc agcactttgc ctgctaccgc cgaggccagc ggattaccgc cataggtggt
    11941 gccatgagtg ccaacggtca tcacgcgggc gcactcttcg gttgccaaca acgcaccgac
    12001 cgggaaaccg ccgcccagcg ctttggcggt ggttaacaga tcgggcgtca cgccgtagtg
    12061 catataggca tacagctcac cagtgcgccc gacgccggtt tgtacctcat caaaaatcag
    12121 caaggcattg tggcggtcac acaattcacg cagaccttgt aaaaacgcgt tgctggctgg
    12181 caccacaccg ccttcgccct agatgggctc gacaatcacc gcacaggtgg cgtcgtcaat
    12241 cagcgcgctg gcagagttaa tatcgttata tgcagcatga cgaatatccg ccggcagtgg
    12301 cgcaaaatcc tgtgaatagg ctggctgccc acccgcactg acagtaaaca gcgtgcgacc
    12361 atgaaacgca tttttgaacg ccacgatgcc actcttatgg ctgccgtagc ggtcgtgagc
    12421 gaatttacgc gccagtttta gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa
    12481 gacgcgatcg gcaaacgtgg cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt
    12541 ggtgtaaccg ttgccggtat gccagaactt actcgcctgt tcgttcagcg cttcacgcag
    12601 ttccggatgc gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc
    12661 tttcccctgc tgatcccaca agcgcgaacc ttcgccacgt accggtataa agggtgccgg
    12721 agcgtaaaca ggtatcatcc attcatcaaa gttttcacgc gtaattggct gagacatagc
    12781 gacctctaca gtaaataatt cgttatttgt atgttaacaa taagtaatgt ttgcgctgta
    12841 aatgtagatt gcagggttcg tgccagccag tgataaaagt gcataaacgg cggaggctaa
    12901 ctggaaatca aggagttata accaaaccat atgcatttaa agtgcatata aagtgaatac
    12961 gtttgcgatg tgggtgaata aaaagaataa aaaacgcaat gttatgcaga agtaaaatat
    13021 aattctagaa ttgtgatcat tgacgaaatt tactggaaat tactgcgcca ttctgacgca
    13081 gcgcgcacca aaagcgggca ttttttgcgc catcgttgac atcattaaca accatcgatc
    13141 aaatcactta acaacaggcg gtaagcaacg cgaaattctg ctaccatcca cgcactcttt
    13201 atctgaataa atggcagcga ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc
    13261 cagacctcac cagcttgaag ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca
    13321 ggagacaaaa gttcatgacg atatgtttcc gctcgaagag gtggcgaagc tcggctacaa
    13381 cgtgttttat cacgggcaga aaggccatta tggcgtggcg ctgctgacca aagagacgcc
    13441 gattgccgtg cgtcgcggct ttcccggtga cgacgaagag gcgcagcggc ggattattat
    13501 ggcggaaatc ccctcactgc tgggtaatgt caccgtgatc aacggttact tcccgcaggg
    13561 tgaaagccgc gaccatccga taaaattccc ggcaaaagcg cagttttatc agaatctgca
    13621 aaactacctg gaaaccgaac tcaaacgtga taatccggta ctgattatgg gcgatatgaa
    13681 tatcagccct acagatctgg atatcggcat tggcgaagaa aaccgtaagc gctggctgcg
    13741 taccggtaaa tgctctttcc tgccggaaga gcgcgaatgg atggacaggc tgatgagctg
    13801 gggggtggtc gataccttcc gccatgctaa tccgcaaaca gcagatcgtt tctcatggtt
    13861 tgattaccgc tcaaaaggtt ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc
    13921 cagccaaccg ctggcagaat gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat
    13981 ggaaaaaccg tccgatcacg cccccgtctg ggcgaccttc cgccgctaat ttagcagctc
    14041 tcctggctca aactgggtca ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa
    14101 tgatttgttg cctgccgtcc tttgttatac cgtcactgcg tttttagttg tctgaccact
    14161 tctctattat caagtttgat ataggaaact ccacgatgaa cgctgagcgt aactttctgt
    14221 ttgcctgtct tatttttgcg ctggtcattt acgctatcca cgctttcggt ttattcgatc
    14281 tgctcaccga tttaccccac ttacagacac tcattcgcca gagcggattg ttcggttata
    14341 gcctctatat tctgttattc atcaatgccc ccctctttct gttaccagga agcatactgg
    14401 tgatcgctgg aggaatagtt tttggcccgc tcttagggac acaactctca ttaattgccg
    14461 caacgctggc ctcctcgtgc tcattcctgc tggcgcgctg gctgggacgt gatttactgc
    14521 tgaaatacgt gggccatagc cataccttcc aggccattga aaaaggcatt gcgcgtaacg
    14581 gtattgattt tcttattctg acccgcttaa tcccgttgtt tccttacaat attcaaaatt
    14641 acgcttacgg attcaccacc atcgccttct ggccttatac ccttatttcg gcactcacga
    14701 ccctgcccgg tattgttatt tataccgtga tggcaagcga tctcgccaat gaaggcatta
    14761 cgctgcgctt tattttacaa ctctgtctgg cgggcctggc gctgtttatt ctcatccagc
    14821 tcgcaaaact actctacgcc cgacacaaac atgtggatct gtctgcttcg cgccgcagcc
    14881 cacttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc agtgtcatgg
    14941 aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg ctgcgatcaa
    15001 aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca accgctgcgc
    15061 gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg tttcctcacc
    15121 gacgacactc gtcacggtat tgtgtttaaa gatggctcca acggacataa atcgctgttt
    15181 atggcttatg cgcccccgaa agcattttat gaagccctga aagaggcagg tggtacgccg
    15241 ggcgaaaaca tgacgatgga taataaagaa acgactcatg tcacaggcag caaactggat
    15301 atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt gattgttgac
    15361 agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc agaagagaag
    15421 aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag caatgcaaca
    15481 tacacttatg gtgcggtgga aaaacacggc gaagtgaaat tcaaaggcaa tgcctcagtt
    15541 ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata aagccaagat
    15601 aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc ctcatcatcc
    15661 tgttgttcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag tttatcaatc
    15721 gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt attcagtctt
    15781 acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc attgctgcac
    15841 cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg ttctggggcg
    15901 gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt atcgccagag
    15961 tgatgggccg cgaagtggtg gaaaaattaa
//
LOCUS       DAJTYH010000088        15566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTYH010000088 DAJTYH010000000
VERSION     DAJTYH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(115..1224)
                     /gene="anmK"
                     /locus_tag="OO609_002919"
     CDS             complement(115..1224)
                     /gene="anmK"
                     /locus_tag="OO609_002919"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6634259.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1498..1965
                     /gene="slyB"
                     /locus_tag="OO609_002920"
     CDS             1498..1965
                     /gene="slyB"
                     /locus_tag="OO609_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6634260.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2012..2446)
                     /gene="slyA"
                     /locus_tag="OO609_002921"
     CDS             complement(2012..2446)
                     /gene="slyA"
                     /locus_tag="OO609_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6634261.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2647..2883
                     /locus_tag="OO609_002922"
     CDS             2647..2883
                     /locus_tag="OO609_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6634262.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2886..3743
                     /locus_tag="OO609_002923"
     CDS             2886..3743
                     /locus_tag="OO609_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6634263.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3743..5755
                     /locus_tag="OO609_002924"
                     /pseudo
     CDS             3743..5755
                     /locus_tag="OO609_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5756..6277)
                     /gene="sodC"
                     /locus_tag="OO609_002925"
     CDS             complement(5756..6277)
                     /gene="sodC"
                     /locus_tag="OO609_002925"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6634264.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6358..7254)
                     /locus_tag="OO609_002926"
     CDS             complement(6358..7254)
                     /locus_tag="OO609_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6634265.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7303..7542)
                     /locus_tag="OO609_002927"
     CDS             complement(7303..7542)
                     /locus_tag="OO609_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6634266.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7645..8244
                     /gene="nemR"
                     /locus_tag="OO609_002928"
     CDS             7645..8244
                     /gene="nemR"
                     /locus_tag="OO609_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6634267.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8281..9378
                     /gene="nemA"
                     /locus_tag="OO609_002929"
     CDS             8281..9378
                     /gene="nemA"
                     /locus_tag="OO609_002929"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6634268.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9459..9866
                     /gene="gloA"
                     /locus_tag="OO609_002930"
     CDS             9459..9866
                     /gene="gloA"
                     /locus_tag="OO609_002930"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6634269.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            9969..10616
                     /gene="rnt"
                     /locus_tag="OO609_002931"
     CDS             9969..10616
                     /gene="rnt"
                     /locus_tag="OO609_002931"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6634270.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10709..12823
                     /locus_tag="OO609_002932"
     CDS             10709..12823
                     /locus_tag="OO609_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6634271.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12833..14125
                     /locus_tag="OO609_002933"
     CDS             12833..14125
                     /locus_tag="OO609_002933"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634272.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15075..15167
                     /locus_tag="OO609_002934"
     CDS             15075..15167
                     /locus_tag="OO609_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR6634273.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15211..>15566)
                     /locus_tag="OO609_002935"
     CDS             complement(15211..>15566)
                     /locus_tag="OO609_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6634274.1"
                     /translation="GRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAY
                     TSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSG
                     RSLKQEPTEMIQATA"
BASE COUNT         3557 a         4129 c         4088 g         3792 t
ORIGIN      
        1 agttttttca tagtcatatc ccgaagactt tcctggtccg gagggcaaat accctcctta
       61 atcttccaag tgtaacggca gacacgataa gaaaaaattc agttaattct gatatcacga
      121 gttggcgggg aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag
      181 atttcctggt aatcccgcca gcgtccgcca ggcaagccag gcgaaagcca atgcttccat
      241 gtcatcgcca ctaatgccaa cggcatcggt ggtggtgact tctgtgccta gcagtaatac
      301 cgccagacgc gccatgagta gcggattacg actacctcca ccacatacca tcaatcgttc
      361 gcagccaccg ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt
      421 ggcctgaaca tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc
      481 gtagttaaag tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat acgggtcgct
      541 gagcatattt tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt
      601 atcgtaaggt ttacctgcct gacgccagat ccaggcatcc atcagcatgt taccaggacc
      661 ggtatcgtag ccgccaacca gctgcccagg aatgagcagt gacagattgg cgatgccgcc
      721 aatattgagc accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg
      781 taccagcggt gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac
      841 cgtaattccg gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc
      901 cacgcccgtc ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct
      961 cgcctgcagg ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag
     1021 ttgagtatca agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag
     1081 tacagcctgt ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg
     1141 gtgttcatca attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac
     1201 gccaataaag cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac
     1261 tcaaacataa acttttcgtg aataccatgc ggaatgaccg atttttaccg ttggtagtaa
     1321 aacattatct tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac
     1381 tttcattatg atttgctcat atttggtaga acatgtaacc atggattcac atatgccata
     1441 tactttgacc atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg
     1501 attaaacgcg tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac
     1561 gacaccctgt caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc
     1621 tatggcacca tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt
     1681 atcggtgcaa ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga
     1741 accgggcgtt ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc
     1801 gtacagagtg caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt
     1861 aataccatca tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc
     1921 gtactggcca gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg
     1981 gtcaggttac tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat
     2041 tatgctcaag ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat
     2101 gtaatatttc cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg
     2161 ccttttccgt cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa
     2221 ttaacccttt ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa
     2281 tcgcttttgc cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg
     2341 taacccaatg ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc
     2401 atatgcgcac caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat
     2461 aattagcttg ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa
     2521 aacaaatgta ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa
     2581 tttttcgcac ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc
     2641 tactttgtga agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt
     2701 gcgtccgtct attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt
     2761 gtcgtacacc gcctgcttcg tggctggatc tacgccggcg atatctggca tcccttgtta
     2821 atggatttat cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg
     2881 taactatgtc aattaaaaca attaagtatt tctcaacaat cattgtagcg atagttgcgg
     2941 ttcttgccgg atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga
     3001 aaatacgcgc agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga
     3061 atataaaaga caaccaactg gttaacgcgg gggaacttct gctcacgatc gataaaacgc
     3121 cctttcagat cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg
     3181 caaaagccaa taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttttgccg
     3241 aagaactcga taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac
     3301 aggcgacgct gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga
     3361 gtggatgggt gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc
     3421 tgtttgccct tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt
     3481 tgcgccatat ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt
     3541 tacagggtca cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact
     3601 caagccttat tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caactcgttc
     3661 ccgtccgctt tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct
     3721 gtagcattgc cgtaggtcaa taatgaacgc atcgtcatgg tccttgcgca atttgccctg
     3781 gttcagggcc acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct
     3841 ggcgctgacg gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc
     3901 tgcagtggtt agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatggc
     3961 tggcagttta ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc
     4021 gtggtttttt ctattgagca tgtcggcgtg gcttggcttt tgtacctagg cctgtgcgca
     4081 cttcacgaat aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc
     4141 ctttccgatg gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt
     4201 ttgcgaggtg atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag
     4261 ttccgatgct actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca
     4321 tgccagttta ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt
     4381 gattgggcag atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg
     4441 ttttcgccag caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag
     4501 tgtcatctcc agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg
     4561 agaaattctc gaacaattgc tgacggcgct cgccagttcg caaacagatg cttacaccgt
     4621 cgcacgtatt atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt
     4681 ctggcagcga ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg
     4741 tctgcaaagc ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca
     4801 taccgacaac gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat
     4861 tggcgcatgg agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc
     4921 aattagctgc gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat
     4981 gcgcacgctg gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca
     5041 gattagcgat ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct
     5101 tcttaaattg cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg
     5161 ttcgtttatc gctgtcacta atcccccggt gtatgatttt gctgattttc ttaactataa
     5221 tctggcaaaa atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg
     5281 atcggatgct cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca
     5341 gctaagccgc catccaacac tgagtgaaag cgaatttgaa tcgctcactt atcatcacgt
     5401 cagtcagttg agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt
     5461 agtgctgctg aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga
     5521 tccgttatcg cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg
     5581 tggcgttcag caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag
     5641 ccttgcccgt catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta
     5701 ctgctcgctt tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact
     5761 taattacacc acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca
     5821 tattatcgcc gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca
     5881 gacgaggcgc gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca
     5941 gatcgcctaa atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa
     6001 gatgcccgcc tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc
     6061 ttcctttggc atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg
     6121 aaaactccag acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc
     6181 cttgcgacgt gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa
     6241 caaccagcgc cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt
     6301 cctgaataag tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta
     6361 cggtacgtcg taccccagtg cagctttacg gatacgaaac cattgttgac gggtcatttt
     6421 cagtgtttct gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg
     6481 cagcggctgc gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc
     6541 gtttaactcc tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt
     6601 aaacagacga ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag
     6661 ttggtcgagc gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt
     6721 agtggcaagg gtaaacggca gacgtgattg caacagggca aattgcgcag gcgtaaagtt
     6781 cgatacgcca aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac
     6841 ttcatcggca tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt
     6901 cgcgagatta attagcgact gttcggcgct cttaatgatg tgattgcggt cagtgatgta
     6961 atgaccaatg acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc
     7021 catccgttca cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg
     7081 atagccacca taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc
     7141 aataaaactg accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat
     7201 cacaaaacgg gaaaactccg ggccttccgg cgcaatagta atacgctgaa ccataatcgc
     7261 ttcctcttat cagatataag aggagtatac gcaagattag gttcaaaaga gtgatggttg
     7321 ctccggttcg tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg
     7381 acaaaggcgc agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc
     7441 atcccgacta cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg
     7501 gcacgggctc tggacgggaa agaactctaa ttgctccgcc acctcgccct cctcagataa
     7561 gattattacc attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc
     7621 aactggtcta ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat
     7681 ctcttggcga cgggcgagca actttgcctg caacgtggat tcaccgggat ggggctaagc
     7741 gaattactaa aaaccgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa
     7801 gaagcgtttg gcgttgccat gcttgagcgt cattacgccg catatcacca gcgactggct
     7861 gagttgctgc aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa
     7921 acactgaacc agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct
     7981 gccgaagtgt gcgatctgtc agaagatatg cgcagcgcga tggataaagg tgctcgcggc
     8041 gtgatcgccc tgctctcgca ggctctggaa aatggccgtg agaaccattg tttaaccttt
     8101 tgtggcgaac cgctacaaca ggcacaagtg ctttacgcac tatggcttgg cgcgaatctg
     8161 caggccaaaa tttcgcgcag tttcgagcca ctggaaaacg cgctggccca tgtaaaaaac
     8221 attattgcga cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt
     8281 atgtcatctg aaaaactgta ttccccactg aaagtgggcg cgatcacggc ggcaaaccgt
     8341 atttttatgg caccgctgac gcgtctgcgc agtattgaac cgggtgacat tcctaccccg
     8401 ttgatggcgg aatactatcg ccaacgtgcc agtgccggtt tgattattag tgaagccacg
     8461 caaatttctg cccaggcaaa aggatatgca ggtgcgcctg gcatccatag tccggagcaa
     8521 attgccgcat ggaaaaaaat caccgctggc gttcatgctg aaaatggtca tatggccttg
     8581 cagctgtggc acaccggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg
     8641 gtagcgcctt cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag
     8701 gcgatccgcg ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccgggtatc
     8761 gtcaatgatt tccgtcaggc cattgctaac gcgcgtgaag ccgcttttga tctggtagag
     8821 ctccactctg ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt
     8881 accgatcagt acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat
     8941 gccgggattg aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact
     9001 ttccagaaca cagataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa
     9061 ctgggtaaac gcggcattgc ttatctgcat atgtcagaac cagattgggc ggggggtgaa
     9121 ccgtatactg atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc
     9181 gcaggtgcat acacagtaga aaaagctgaa acgctgatcg gcaaagggtt aattgatgcg
     9241 gtggcatttg gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagctcaaa
     9301 gctgagctta acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc
     9361 gattacccga cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac
     9421 aacattttga atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc
     9481 tgcgcgttgg cgatttgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac
     9541 tgctgcgtac cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc
     9601 cggaaaccga agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac
     9661 tcggcactgc ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa
     9721 aaatccgtca aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta
     9781 cggttatcgc gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag
     9841 acgccggtcg cggtctaggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat
     9901 ctttactgca tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag
     9961 agaatcagat gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt
    10021 atcctgttgt gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg
    10081 agattgccgc catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat
    10141 tacatttcca cgtcgaacca tttgtcggcg caaatttgca accagaagcc ctcgccttca
    10201 acggcattga cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg
    10261 aaatttttaa agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg
    10321 tggcgcacaa cgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcgc
    10381 tgaaacgtaa cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg
    10441 cactcggaca aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca
    10501 cccaggcgca ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg
    10561 tcaaccgctg gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga
    10621 gtcgatgcct gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca
    10681 agcatgatat tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct
    10741 gccggacgtg ttttcaccgg cgacccgcga ctggtttctt cgcgccttta aacagccgac
    10801 cgctgtccag tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc
    10861 accgactggc tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg
    10921 cgaaggcggc gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat
    10981 ttcaccgata aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg
    11041 tattgccgat gaacggcggc ggcgcggcga aacggtaatt attgatgaag tccacgcggt
    11101 agcgggcagt aaacgtggtg cgcatctggc gttaagtctg gagcggctcg atgcgctgct
    11161 ccacacctca gcacagcgaa ttggcctttc tgccactgtg cgctcagcca gcgatgtggc
    11221 agcatttctt ggtggcgatc gcccggttac ggtagtcaac ccgcccacaa tgcgccatcc
    11281 gcagatacga attgtcgtac cggtcgccaa tatggatgat gtctcatcgg tcgccagcgg
    11341 caccggcgaa gacagccatg ccggccggga aggctccatc tggccatata ttgaaacggg
    11401 tatccttgat gaagtgttgc gccatcgctc gaccattgtc tttactaatt cacgcgggct
    11461 ggcggaaaaa ctgacggcac gattaaatga gctttacgcc gcacgcttac agcgttcccc
    11521 gtctatcgcc gttgatgcgg cccatttcga gtcgacctcc ggcgcaacct ctaaccgtgt
    11581 acaaagtagc gacgttttta ttgcccgctc acaccacggc tccgtctcta aagaacaacg
    11641 agcaatcacc gaacaggcgc tgaaatcggg tgaattacgc tgcgtggtcg caacctccag
    11701 tcttgaactg gggattgata tgggcgcggt ggatctggtg attcaggtgg caacgccgct
    11761 ttctgttgcc agtgggttac aacgcattgg tcgcgccgga catcaggttg gcggtgtatc
    11821 taaagggctg tttttccccc gtacccggcg tgatttagtc gattccgcag tcattgtaga
    11881 gtgtatgttc gcaggcaggc tggaaaacct gacaccaccg cataatcctc tcgacgtcct
    11941 tgcgcagcaa accgttgccg ccgcggcgat ggatgcatta caggtagacg aatggtactc
    12001 ccgcgtacgc cgtaccgcac cgtggaaaga tctgccaaga cgtgtttttg acgccacgct
    12061 ggatatgctt tccgggcgct atccctctgg cgatttttct gcttttcgcc ccaaactggt
    12121 ctggaacagg gagaccggga tattgaccgc ccgacctggc gctcaattgt tggcggttac
    12181 cagcggcggc accattccgg atcgtggcat gtatagcgtg ttattacccg aaggtgaaga
    12241 aaaggccggt tcgcggcggg tgggtgaact ggatgaggag atggtatatg agtcgcgggt
    12301 gaacgacatt atcactctcg gcgctacctc atggcggatc cagcaaatca cccgcgatca
    12361 ggtgattgtg actcctgctc cgggtcgttc tgcccagctc cccttctggc gtggtgaagg
    12421 taacggacgt ccggctgaat taggcgagat gatcggcgat tttcttcatt tactggcgga
    12481 tggcgcgttc ttttccggga ctattccccc gtggctggca gaagaaaata cgaacgccaa
    12541 tattcagggg ttgattgacg agcaacgcaa cgcgacgggc atcgttccgg ggagtcgcca
    12601 tctggtcctc gaacggtgcc gtgatgaaat tggcgactgg cgtattattt tgcactctcc
    12661 ctatggaaga cgggtgcatg aaccctgggc gctggcgatt gccgggcgaa tacatgccct
    12721 atggggcgct gacgcgtctg tggtcgccag tgatgacagc attgttgcgc gcattcctga
    12781 cactgatggt aaattgcccg acgccgcgat ttttttgttt taaccagaaa agttgctgca
    12841 aattgtccgc gaggcggtag gcagctcggc acttttcgcc gcccgttttc gcgaatgcgc
    12901 cgcgcgggca ttattaatgc cgggccgcac gccgggccat cgcaccccgc tttggcaaca
    12961 acggctgcgc gccagtcagt tgctggaaat cgctcaggga tatccggatt ttccggtcat
    13021 tctcgaaacc ctacgcgaat gtctgcaaga tgtttatgat cttcccgcac tggaacgttt
    13081 gatgcgtcgc ctgaacggtg gcgaaattca aatatccgat gtaacgacca ctacgccctc
    13141 gcctttcgcc acaagtttat tgttcggcta tgtcgcggaa tttatgtacc agagcgacgc
    13201 cccgctggca gagcgccggg catccgtact gtcgctggac agcgagttac tgcgcaatct
    13261 actcggacag gtcgatccgg gggaattact cgacccgcag gtcattcgcc aggtggaaga
    13321 agagttgcaa cgactggctc ctggtagaag agcgaaaggt gaagaaggat tgttcgacct
    13381 gctgcgcgaa ctggggccaa tgaccgttga agacctggcg caacggcata caggcagcag
    13441 tgaagaggtt gcgtcgtatc tggaaaatct tcttgcagta aaacgaatct tcccagcgat
    13501 gattagcgga caggagcgtc ttgcctgtat ggatgatgcc gccaggctgc gtgatgccct
    13561 cggcgtacgg ctaccagagt cattgccaga gatttattta catagagtca gttacccgct
    13621 tcgcgacctc tttctgcgct atctccgggc tcatgctctg gtcacggctg aacaactggc
    13681 tcatgagttt agtctcggta ttgccattgt cgaagagcag cttcagcaac tgcgtgaaca
    13741 gggtctggtg atgaatctgc aacaagacat ctgggtgagc gatgaagtat ttcgtcgtct
    13801 gcgtttgcgc tcgctgcaag ccgccagaga agcgacgcgt cccgttgcag ccacgaccta
    13861 tgcgcgattg ctgctggaac gtcagggcgt attacccgcc accgatggta gcccggcgct
    13921 ctttgcctca acatctccag gcgtttatga gggcgtagat ggagtgatgc gggtgatcga
    13981 acagcttgcc ggagtcggtt tacccgcctc actctgggaa agccagatcc tgcctgcccg
    14041 cgtacgcgac tattcgccag aaatgctcga tgaattactg gcaaccggag cggttatctg
    14101 gtcgggacaa aaaaaagctg ggtgaagatg acggcctggt ggcactgcat ctacagaaat
    14161 atgccgcaga atcgttcact cccgccgaag cggatcaggc gaatcgttcg gcgctgcagc
    14221 aagcgataat cgctgttctg gctgacggag gagcctggtt tgcacaacaa atcagccaac
    14281 ggatacgcga taaaatcgtc gaatcggttg attcctcggc actgcaagag gcgttatggg
    14341 cgctggtctg gcaaggcgtc attaccagcg atatttgggc accgttacgc gccctcaccc
    14401 gcagcagttc caacgcacgc acctcaactc gccgcagtca ccgggctcgt cgtgggcgtc
    14461 ctgtctatgc acaacccgtc tcgccgcggg tatcttacaa cacaccaaat ctggctggac
    14521 gctggtcgtt attgcaggtg gagccactaa acgataccga aaggatgctg gcgctggcgg
    14581 aaaatatgct cgaccgctac ggcatcatca gtcgtcaggc ggtgatagcc gaaaatatcc
    14641 ctggcgggtt tccatcgatg caaacacttt gtcgaagtat ggaagactcc gggcgaatta
    14701 tgcgaggtcg ttttgtagaa ggtctgggtg gcgcgcaatt cgctgaacgt ctgactattg
    14761 accgattgcg cgatctggcg acacaagcca cgcaaacacg ccactataca ccagtggcgc
    14821 tctccgccaa cgatccggct aatgtgtggg gaaatcttct gccctgggct gcacatccgg
    14881 caacgctggt accaacgcgt cgggcgggtg cgctggtggt cgtttctggc ggcaaattgt
    14941 tactctatct ggcgcaaggc ggcaaaaaaa tgctggtctg gcaggaaaag gaggaattac
    15001 tcgccccaga gattttccag gcgctgacca ccgcactgcg tcgcgaacca aggctgcgct
    15061 ttacgctaac agaagtgaat gatcttccgg tccggcaaac gccgatgttt acgctgctgc
    15121 gtgaggcggg attttcaagt tcgccacaag ggctggattg gggatagttg acatcctcca
    15181 cgccctgaaa gacgtggttc cctgctacgt tcaagctgtc gcctgaatca tttcggtggg
    15241 ttcctgcttc aacgagcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc
    15301 cgccgctaaa atgttacgag cgccatttac atcggcgttc gctgtatatc cacatacctg
    15361 gcatctgaat ttactttgtg acaggcggtt ttctttcgct gtatgaccac agcacgcgca
    15421 acgctggctt gtgtacgctg gcggaacagc aagcacctga ccgccactcc agagctgctt
    15481 gtactcaagc tggcggcgca tttcatacca gccctgatcc agtatcgaac ggtttaaacc
    15541 tgattttgcc cggacattgc gccccg
//
LOCUS       DAJTYH010000089        15330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTYH010000089 DAJTYH010000000
VERSION     DAJTYH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO609_002936"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO609_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6634275.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO609_002937"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO609_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6634276.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO609_002938"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO609_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO609_002939"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO609_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6634277.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO609_002940"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO609_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6634278.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO609_002941"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO609_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6634279.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO609_002942"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO609_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6634280.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO609_002943"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO609_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6634281.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO609_002944"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO609_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6634282.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO609_002945"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO609_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6634283.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO609_002946"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO609_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6634284.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO609_002947"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO609_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6634285.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO609_002948"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO609_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6634286.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO609_002949"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO609_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6634287.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO609_002950"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO609_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6634288.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO609_002951"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO609_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6634289.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO609_002952"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO609_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6634290.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO609_002953"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO609_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6634291.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO609_002954"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO609_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6634292.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO609_002955"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO609_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6634293.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO609_002956"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO609_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6634294.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO609_002957"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO609_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6634295.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO609_002958"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO609_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6634296.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO609_002959"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO609_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6634297.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO609_002960"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO609_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6634298.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO609_002961"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO609_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6634299.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO609_002962"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO609_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6634300.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO609_002963"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO609_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6634301.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO609_002964"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO609_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6634302.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO609_002965"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO609_002965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6634303.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..>15330
                     /gene="rplQ"
                     /locus_tag="OO609_002966"
     CDS             15058..>15330
                     /gene="rplQ"
                     /locus_tag="OO609_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6634304.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRA"
BASE COUNT         3959 a         3596 c         4082 g         3693 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctaaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaagccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc
//
LOCUS       DAJTYH010000090        15077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTYH010000090 DAJTYH010000000
VERSION     DAJTYH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..1117)
                     /locus_tag="OO609_002967"
     CDS             complement(<1..1117)
                     /locus_tag="OO609_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6634305.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQER"
     gene            complement(1316..1400)
                     /locus_tag="OO609_002968"
     tRNA            complement(1316..1400)
                     /locus_tag="OO609_002968"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1364..1366),aa:Leu,seq:caa)
     gene            1596..2615
                     /gene="ahr"
                     /locus_tag="OO609_002969"
     CDS             1596..2615
                     /gene="ahr"
                     /locus_tag="OO609_002969"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6634306.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2744..4246
                     /locus_tag="OO609_002970"
     CDS             2744..4246
                     /locus_tag="OO609_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6634307.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4365..5447)
                     /gene="lptG"
                     /locus_tag="OO609_002971"
     CDS             complement(4365..5447)
                     /gene="lptG"
                     /locus_tag="OO609_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6634308.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5447..6547)
                     /gene="lptF"
                     /locus_tag="OO609_002972"
     CDS             complement(5447..6547)
                     /gene="lptF"
                     /locus_tag="OO609_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6634309.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6814..8325
                     /gene="pepA"
                     /locus_tag="OO609_002973"
     CDS             6814..8325
                     /gene="pepA"
                     /locus_tag="OO609_002973"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6634310.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8459..8902
                     /gene="holC"
                     /locus_tag="OO609_002974"
     CDS             8459..8902
                     /gene="holC"
                     /locus_tag="OO609_002974"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6634311.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8902..11757
                     /gene="valS"
                     /locus_tag="OO609_002975"
     CDS             8902..11757
                     /gene="valS"
                     /locus_tag="OO609_002975"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6634312.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11811..13007)
                     /locus_tag="OO609_002976"
     CDS             complement(11811..13007)
                     /locus_tag="OO609_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6634313.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13200..13703
                     /locus_tag="OO609_002977"
     CDS             13200..13703
                     /locus_tag="OO609_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6634314.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13749..14165)
                     /gene="rraB"
                     /locus_tag="OO609_002978"
     CDS             complement(13749..14165)
                     /gene="rraB"
                     /locus_tag="OO609_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6634315.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14327..>15077
                     /gene="argF"
                     /locus_tag="OO609_002979"
     CDS             14327..>15077
                     /gene="argF"
                     /locus_tag="OO609_002979"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6634316.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIAL"
BASE COUNT         3882 a         3853 c         3886 g         3456 t
ORIGIN      
        1 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
       61 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      121 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      181 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      241 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      301 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      361 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      421 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      481 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      541 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      601 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      661 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      721 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      781 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      841 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      901 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
      961 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1021 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1081 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1141 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1201 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1261 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1321 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1381 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1441 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1501 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1561 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1621 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1681 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1741 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1801 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1861 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1921 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     1981 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2041 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2101 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2161 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2221 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2281 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2341 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2401 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2461 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2521 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2581 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2641 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2701 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2761 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2821 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2881 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     2941 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3001 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3061 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3121 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3181 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3241 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3301 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3361 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3421 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3481 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3541 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3601 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3661 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3721 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3781 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3841 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3901 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     3961 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4021 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4081 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4141 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4201 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4261 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4321 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4381 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4441 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4501 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4561 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4621 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4681 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4741 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4801 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4861 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4921 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     4981 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5041 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5101 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5161 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5221 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5281 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5341 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5401 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5461 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5521 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5581 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5641 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5701 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5761 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5821 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5881 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     5941 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6001 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6061 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6121 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6181 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6241 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6301 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6361 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6421 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6481 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6541 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6601 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6661 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6721 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6781 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6841 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6901 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     6961 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7021 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7081 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7141 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7201 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7261 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7321 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7381 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7441 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7501 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7561 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7621 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7681 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7741 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7801 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7861 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7921 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     7981 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8041 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8101 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8161 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8221 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8281 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8341 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8401 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8461 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8521 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8581 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8641 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8701 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8761 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8821 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8881 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     8941 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9001 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9061 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9121 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9181 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9241 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9301 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9361 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9421 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9481 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9541 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9601 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9661 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9721 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9781 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9841 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9901 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
     9961 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10021 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10081 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10141 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10201 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10261 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10321 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10381 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10441 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10501 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10561 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10621 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10681 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10741 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10801 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10861 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10921 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    10981 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11041 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11101 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11161 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11221 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11281 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11341 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11401 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11461 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11521 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11581 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11641 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11701 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11761 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11821 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11881 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    11941 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12001 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12061 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12121 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12181 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12241 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12301 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12361 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12421 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12481 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12541 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12601 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12661 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12721 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12781 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12841 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12901 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    12961 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13021 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13081 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13141 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13201 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13261 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13321 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13381 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13441 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13501 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13561 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13621 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13681 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13741 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13801 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13861 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13921 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    13981 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14041 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14101 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14161 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14221 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14281 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14341 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14401 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14461 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14521 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14581 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14641 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14701 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14761 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14821 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14881 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    14941 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15001 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15061 tgaacgcatt gcattac
//
LOCUS       DAJTYH010000091        14889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTYH010000091 DAJTYH010000000
VERSION     DAJTYH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO609_002980"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO609_002980"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6634317.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO609_002981"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO609_002981"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6634318.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO609_002982"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO609_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6634319.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO609_002983"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO609_002983"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6634320.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO609_002984"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO609_002984"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6634321.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO609_002985"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO609_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6634322.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO609_002986"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO609_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6634323.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO609_002987"
     CDS             complement(8065..9207)
                     /locus_tag="OO609_002987"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6634324.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO609_002988"
     CDS             complement(9216..10229)
                     /locus_tag="OO609_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6634325.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO609_002989"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO609_002989"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6634326.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO609_002990"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO609_002990"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6634327.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO609_002991"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO609_002991"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6634328.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO609_002992"
     CDS             complement(12827..13951)
                     /locus_tag="OO609_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6634329.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..>14889)
                     /gene="rfaQ"
                     /locus_tag="OO609_002993"
     CDS             complement(13948..>14889)
                     /gene="rfaQ"
                     /locus_tag="OO609_002993"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6634330.1"
                     /translation="VLLYQDTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVL
                     RANKYDLIVNLTDQWMVAILVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVV
                     ESNLSVLTPLGLDSLVKQTTMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCW
                     DNAKFSAVIDALHARGYEVVLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELG
                     ALIDHAQLFIGVDSAPAHIAAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMP
                     TRDQRDRNEMYLSVIPAADVIAAVDKLLPSSTTGTSL"
BASE COUNT         4226 a         3353 c         3067 g         4243 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg aaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgg cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaatatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcac
//
LOCUS       DAJTYH010000092        14185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTYH010000092 DAJTYH010000000
VERSION     DAJTYH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..563
                     /gene="fepE"
                     /locus_tag="OO609_002994"
     CDS             <1..563
                     /gene="fepE"
                     /locus_tag="OO609_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6634331.1"
                     /translation="IDYISALVVKESIENVRNKLEIKTQFEKEKLAQDRIKTKNQLDA
                     NIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGADGIERKLEIEKAVTDVAE
                     LNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKDGPGKAIIVILSALIGGM
                     VACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(560..1375)
                     /gene="fepC"
                     /locus_tag="OO609_002995"
     CDS             complement(560..1375)
                     /gene="fepC"
                     /locus_tag="OO609_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6634332.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(1372..2364)
                     /gene="fepG"
                     /locus_tag="OO609_002996"
     CDS             complement(1372..2364)
                     /gene="fepG"
                     /locus_tag="OO609_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6634333.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(2361..3365)
                     /gene="fepD"
                     /locus_tag="OO609_002997"
     CDS             complement(2361..3365)
                     /gene="fepD"
                     /locus_tag="OO609_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6634334.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            3476..4708
                     /gene="entS"
                     /locus_tag="OO609_002998"
     CDS             3476..4708
                     /gene="entS"
                     /locus_tag="OO609_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6634335.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGTAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(4712..5668)
                     /gene="fepB"
                     /locus_tag="OO609_002999"
     CDS             complement(4712..5668)
                     /gene="fepB"
                     /locus_tag="OO609_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6634336.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            5960..7135
                     /gene="entC"
                     /locus_tag="OO609_003000"
                     /pseudo
     CDS             5960..7135
                     /gene="entC"
                     /locus_tag="OO609_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7145..8755
                     /gene="entE"
                     /locus_tag="OO609_003001"
     CDS             7145..8755
                     /gene="entE"
                     /locus_tag="OO609_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6634337.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            8769..9626
                     /gene="entB"
                     /locus_tag="OO609_003002"
     CDS             8769..9626
                     /gene="entB"
                     /locus_tag="OO609_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6634338.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            9626..10372
                     /gene="entA"
                     /locus_tag="OO609_003003"
     CDS             9626..10372
                     /gene="entA"
                     /locus_tag="OO609_003003"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6634339.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            10375..10788
                     /gene="entH"
                     /locus_tag="OO609_003004"
     CDS             10375..10788
                     /gene="entH"
                     /locus_tag="OO609_003004"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6634340.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            10969..13074
                     /gene="cstA"
                     /locus_tag="OO609_003005"
     CDS             10969..13074
                     /gene="cstA"
                     /locus_tag="OO609_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6634341.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            13256..13453
                     /locus_tag="OO609_003006"
     CDS             13256..13453
                     /locus_tag="OO609_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6634342.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(13463..>14185)
                     /gene="hcxA"
                     /locus_tag="OO609_003007"
     CDS             complement(13463..>14185)
                     /gene="hcxA"
                     /locus_tag="OO609_003007"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6634343.1"
                     /translation="CAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIILNAPQQY
                     LLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALADQQNQQ
                     LTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGTKVAYGI
                     LVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRPVESIHY
                     LPVTLTPDTLRAAFEKVESFKA"
BASE COUNT         3091 a         3749 c         4127 g         3218 t
ORIGIN      
        1 atatcgatta tatctctgcg ttggtggtga aagagtcgat agaaaacgtc cgtaataaac
       61 tggagatcaa aacccagttt gaaaaagaaa aactggctca ggatcgcatt aaaacgaaaa
      121 atcaacttga tgcaaacatt cagcgcctca attattcact cgacattgcc aacgcggcag
      181 gaattaaaaa gcccgtatac agtaatggtc aggccgttaa agatgacccc gatttctcta
      241 tttccctcgg cgcagacggt attgaacgca aactggaaat tgaaaaagcg gttactgacg
      301 ttgcggaact gaacggtgaa ttacgtaatc ggcagtatct tgtcgagcaa ttaacaaaag
      361 caaatgtcaa cgatgtgaat tttacgccgt ttaaatatca gttaagcccg tcattgccag
      421 tgaaaaaaga cggtccgggt aaggcgatta ttgtgatcct ttccgcgctg atcggcggga
      481 tggtggcttg tggtagcgtg ttattgcgct atgcgatggc atccagaaaa caggatgcca
      541 tgatggcaga ccacttagtt taactatttg cggttgaagg tgccgttcgt ccgagcggca
      601 ccacaagcgg cattccggct accggatcgt caatgatcat gcatcgcaga ccataaatgc
      661 gctcaatcag ttcagcagtg acaatctcct tcggcgctcc ctgagcaaca attttccctt
      721 cccgcaatgc aatcaaatgg ctggcgtaac gacaggcctg attaagatcg tgcagcaccg
      781 ccgccagggt atagcctttc tcgcggttca gttcgcttaa cagttccagt agatcaatct
      841 gatgactgat atccagccag gtcgtgggtt cgtcgagcag cataattgcc gtttcctggg
      901 ccagcaccat cgcgatccac gctcgctggc gttgtccgcc agaaagggta tccacgcttt
      961 gatctgccag atgagttatt cccgtggcct gcatcgcttt cgttaccgct tcttcatcct
     1021 ctttgcgcca gcgggtaaac agcggttgat gcggataacg tccacgcgcc accagctcct
     1081 gcacggtgat atcgcccggc gtggtagcat tttgcgccaa cagaccaatc cggcgtgcaa
     1141 cttctttact ggcgtaatgt tgaatgtgct cgccatccag ccagacatgc ccgtgagcag
     1201 gcgtcatcag gcggcttaag gtacgcagta acgtggattt accacagcca tttggtccga
     1261 taattgccgt gaagtgacca tcaggaattt ctacagtcag attttccgca acggtatatt
     1321 tgccatatcc cagggttaac tgttcgccgc gcaaacgggc tactgattcg gtcatttttt
     1381 gcgagactcc tgaattaaca agacgataag gtaaataccg ccgaggctga cggtaacgac
     1441 gccaaccgga agttgatacg gcataaacag ttgttgggcg cagagaacgg ccgccagcag
     1501 taacagcgct ccgcacagcg ccgcctgggt cagcccccag cgagcggtgc cgctaatgcg
     1561 tcgggcaatg tgcggcgcga ctaaagcaat aaaggaaatc ggcccggcaa gcgccgttgc
     1621 cgcagcggta agcaccactg caaccagcat cattaacaga cgcgaacgct cgacgctgac
     1681 gcccagcgcg caggcggtat catcgcccat ctccagcaag cgcattcgcc gcaccagcaa
     1741 ggtggcggca atgagcatca atataatgat gggtgcggaa ggcgaggttt ttgcccacgt
     1801 caggccgttg agcgatccgg cattccacaa acctgctgtt agcgccgttt ctaaagacgc
     1861 tttcagcaac agccaggtat taaaggccac cagcatggcg cgaacgccga taccgataat
     1921 aatcaaccga aaggtgtcga taccgttacg ccaggcgagc agccagacca acagcgaagt
     1981 gataatgccg cccaccattg ctgccagcgc gatagccgtc agatcctgac caaacagcac
     2041 catcgccacc agcacgccgc tccacgcccc ggtgttaaag cccattacgt cagggctgcc
     2101 gagcgggtta cgcatcagcg actgaaaaat cgcgccgctg acgcccagcg ccgcgccaat
     2161 caacagcgcc atcagcacgc gtggtaaacg ccattcggtg accaccatcg tcatactgcg
     2221 cggcgcatcg cccatcaacg cggcgaatac ctgcgagatt tccagcgtga cggcaccgct
     2281 gcgtaatccc cagatacctg caaccacaca ggcggaaatc agcagcaaac aggtgatgag
     2341 taatcggcga gagacgtaaa tcatgcacca cctcgcgttt tacgtcgcac gaggaagatc
     2401 agcaccggtg caccaataaa cgcactgacc acagaaacgc gcagttcgcc gggcacaatc
     2461 acccgcccga tgatatcggc aaacagcagc agggcagggg tagcaagtag cgtgacgggc
     2521 agcgaccagc gatgatcggc acctaccagc cagcgcgcca tgtgcggcat catcaggccg
     2581 ataaaggcaa tcgggccaac taccgccgtc gcactaccac aaagcacggt aatcgccagc
     2641 agaccaatca actgtgtgcg cgccacgcga ctgcccagcg ccgtcgcggt gtcactgccg
     2701 aggctcaaac tgttcagcgc gcgactcagt aatagcgcag ttgctccggc gatcagaacc
     2761 gggatcagca ccacttttaa ggtatgtaga ttgcgaatat ccagcgaacc ggcttgccag
     2821 aaacgcagtt gatcgtagac gtcaggatta agcagggcga tgccgctggt cagtccttcc
     2881 agcaccgccg ccagcgccac gcccgccagg gttaaacgca ccggacttaa ctgccctcca
     2941 ccctgactgc cggtaaaggc aacaatcaat gaggccacca gcgccccggc gaaggccatc
     3001 gccagttgtt cctgtgcgga tgagtaacca aacagcgccg cacccagcac aatggcaaag
     3061 ctggctccgg cgttcacgcc aagcaagccg gggtcggcaa gtgggtttcg ggtgagggtt
     3121 tgcattaacg ccccggcaag gccaagcgcg ccgcctgcca gtaaaccggc aagggtacgc
     3181 ggcagccgcg cgtcgagcac gatggtgcag tcggcgctct ggcaggtgcc ggagaaggcc
     3241 tccagcacta cggaagcggg gagtgatttt gccccaatga gcaggcttaa tgccgtcgcg
     3301 ataatcagta ataacagcaa tccgggcacg gcaatggcgc gtgtcacggc aacagaacca
     3361 gatatacgaa cttccatgat aatgaaatta attatcgtta tcgatcttat ttggatatgt
     3421 taacatgtgc agcctaagaa taggtattta aaatatttga tggcaaggca ttgtaatgaa
     3481 taaacaatcc tggctgctta acctcagcct gttgaaaacg cacccggcgt ttcgcgcagt
     3541 attcctcgct cgttttatct caattgtgtc tctgggtttg ctcggcgtcg cggtgccggt
     3601 gcagatccag atgatgaccc attctacctg gcaggtgggg ctttcggtga cgctgaccgg
     3661 cggcgcgatg tttgttggcc tgatggttgg cggtgtgctg gcggatcgct atgaacgcaa
     3721 aaaagtgatt ttgctggcgc gcggcacctg tggcattggc ttcattggac tgtgccttaa
     3781 tgcactgctg ccggagccgt cattgctggc aatctattta cttggtttat gggatggttt
     3841 tttcgcatca cttggtgtta cggcgctact ggcggcaaca tctgcactgg tagggcgtga
     3901 aaacttaatg caggccgggg cgatcaccat gttgaccgtg cgcctggggt cggtgatttc
     3961 gcccatgatt ggcggtttat tgctggcgac cggcggcgta gcctggaact acgggctggc
     4021 ggcggcgggc acgtttatta ccttgttacc gttattaagc cttccggagt tgccaccgcc
     4081 accgcagccg cttgagcatc cgttgaaatc attactggca ggatttcgtt ttctgcttgc
     4141 cagcccgctg ctgggtggtt tattgacgat ggcgagcgcg gtgctggtac tgtatccggc
     4201 gctggctgac aactggcaga tgtcagcggc acagattggt tttctctacg cggcgatccc
     4261 gctcggcacg gctattggtg cgttaaccag cgggaagctg gcacatagtg cgcgaccagg
     4321 gttattgatg ctgctctcca cgctgggatc gttcctcgcc attggtctgt ttggcctgat
     4381 gccgatgtgg attttaggcg tggtttgtct ggcgctgttc ggctggctga gcgcggttag
     4441 ttcgttgctg caatatacaa tgctgcaaac gcaaaccccg gaagcgatgt tagggcggat
     4501 taacggtttg tggacggcgc agaacgtgac gggcgatgcc ataggcgcgg cgctgctggg
     4561 tggtttgggc gcgatgatga caccggttgc ttccgcaagc gcgagcggtt ttggtttgtt
     4621 gattatcggc gtgttgttat tgctggtgct ggtggagttg cgacgttttc gccagacgcc
     4681 gccgcaggtg acagcgtccg acagttaatg cttaaaacag cgccttaagc cgttccagca
     4741 cttgcatggc gctgtagtaa tccaggcgga acgtctcggt tcccagcgca taaacctgct
     4801 tgttttgtac cgcaggcagg tgcgcgagca gtggattggc ataaatcgca tcggcatctt
     4861 tctgatcacc agcgaacagg aatagcgatt caccgtttaa ccccgcagcc agattttccc
     4921 caccaagctg aatgatgtca tggcgtttgc cctggctttg gctggcgttt aaccctgcgg
     4981 gcaatttcgc cagcgtaaag ccgagttgtt ccagcatctg cccttgtgct gattccggcg
     5041 tccagagatt ggcactgtgt gcagcggcgg tatagacaat ggcagtgacc ggctgcggcg
     5101 gtaatttgat ttgctctttc gccgctgcca gttgcttatc aaactgtgca atccgctctg
     5161 ccgcttgttt ctcatgcccg gtaatttcgc caagctgcgt taacagcgac tgccagcttt
     5221 tgtcgtcgta attgatgatt aatgtcgggg cgatggtgga aagctgatca tacagcgcca
     5281 gcgccgaatc cccgccggtt gcgctaatta aaatcagatc cggcatttgc gcggcaacgg
     5341 cttcggcgct cggttcgccg atatagagcc gttgcagttt gcgttctttc gccaccttgc
     5401 tccactggcg taaaaagccc tggtcatctg cgacgcggtt attcggcgtg gtcgcgccgc
     5461 tggcgatcac cggagcatca atcgccagca gtgatccggt cagggtgacg ctggtggaaa
     5521 caatacgctg cggctggctt tccagggtat gagtgccccg gctgtcagta atctgacgcg
     5581 gccagtcggc ggcctgaact acggctattc ctgaaagcaa aagtcctgtt aatagaaggg
     5641 cgttgcggta gagcggggcg agtctcacaa gtcagcttcc tgttattaat aaggttaagg
     5701 gcgtaatgac aaattcgaca aagcgcacaa tccgtccctt tggggagagg gggcaaaacg
     5761 gcgcaggaca tcacattgcg cttatgcgaa ttcatcaata atgcttctca ttttcattgt
     5821 aaccacaacc agatgcaacc ccgagttgca gattgcgtta cctcaagagt tgacatagtg
     5881 cgcgtttgct tttaggttag cggccgaaaa tataagtgat aatcattatt aaagccttta
     5941 tcattttgtg gaggatgata tggatacgtc actggctgag gaagtacagc agaccatggc
     6001 aacacttgcg cccaatcgct ttttctttat gtcgccgtac cgcagtttta cgacgtcagg
     6061 atgtttcgcc cgtttcgatg aaccggctgt gaacggggat tcgcccgaca gtcccttcca
     6121 gcaaaaactc gccgcgctgt ttgccgatgc caaagcgcag ggcatcaaaa atccggtgat
     6181 ggtcggggcg attcccttcg atccacgtca gccttcgtcg ctgtatattc ccgaatccta
     6241 gcagtcgttc tcccgtcagg aaaaacagac ctcagcccgc tgttttaccc gcagccagtc
     6301 gctgaacgtg gtggaacgct aggcaattcc tgaacaaacc acgtttgaac agatgggtgc
     6361 ccgcgccgcc gcacttaccg ccacgccgca ggtcgacaaa gtggtgttgt cacgattgat
     6421 tgatatcacc actgacgccg ccattgatag tggcgtattg ctggaacggt tgattgcgca
     6481 aaacccggtt agttacaact tccatgtccc gctggctgat ggtggcgtcc tgctgggggc
     6541 cagcccggaa ctgctgctac gtaaagacgg cgagcgtttt agctccattc cgttagccgg
     6601 ttccgcgcgt cgtcagccgg atgaagtgct cgatcgcgaa gcgggtaatc gtctgctggc
     6661 gtcagaaaaa gatcgccatg aacatgaact ggggactcag gcgatgaaag aggtactgcg
     6721 cgaacgcagt agtgagttac acgttccctc ctctccacaa ttgattacca cgccgacgct
     6781 gtggcatctc gcaactccct ttgaaggtaa agcgaattcg caagaaaacg cactgactct
     6841 ggcctgtctg ctgcatccga cccccgcgct gagcggtttc ccgcatcagg ccgcgaccca
     6901 ggttattgct gaactggagc cgttcgaccg cgaactgttt ggcggcattg tgggttggtg
     6961 tgacagcgaa ggtaacggcg aatgggtggt gaccatccgc tgcgcgaagc tgcgggaaaa
     7021 tcaggtgcgt ctgtttgccg gagcggggat tgtgcctgcg tcgtcaccgt tgggtgagtg
     7081 gcgcgaaaca ggcgtcaaac tttctaccat gttgaacgtt tttggattgc attaaggagc
     7141 gaggatgagc attccattca cccgctggcc ggaagagttt gcccgtcgct atcgggaaaa
     7201 aggctactgg caggatttgc cactgaccga cattctgact cgccacgctg cgagtgacag
     7261 catcgcggtt atcgacggcg agcggcagtt gagttatcgg gagctgaatc aggcggcgga
     7321 taacctcgcc tgtagtttgc gccgtcaggg cattaaacct ggtgaaaccg cgctggtaca
     7381 actgggtaac gtcgctgaac tttacattac ctttttcgcg ctgctgaaac tgggcgtcgc
     7441 gccggtgctg gcgctgttca gccatcagcg tagtgaactg aacgcctacg ccagccagat
     7501 tgaaccggcg ttactgattg ccgatcgcca gcatgcgcta tttagcgggg atgatttcct
     7561 caacacattt gttgcagagc attcttccat tcgcgtggtg caactgcaca acgacagcgg
     7621 tgagcataac ttgcaggatg cgattaacca tccggcagag ggttttactg ccacgccatc
     7681 acctgctgat gaagtggcct atttccagct ttccggcggc accaccggca caccgaaact
     7741 gatcccgcgc actcataacg actattacta cagcgtgcgt cgtagcgccg agatttgtca
     7801 gttcacacaa cagacgcgct acctgtgctc gatcccggcg gctcataact acactatgag
     7861 ttcgccagga tcgctgggcg tctttcttgc cggaggaacg gttgttctgg cggccgatcc
     7921 cagcgccacg ctttgcttcc cgttgattgc aaaacatcag gttaacgtta ccgcgctggt
     7981 gccgccggca gtcagcctgt ggttgcaggc gctggctgaa ggcgaaagcc gggcgcagct
     8041 tgcctcgctg aaactgttac aggtcggcgg cgcacgtctt tctgccacgc ttgcagcgcg
     8101 tattcccgct gagattggct gccagttgca gcaggtgttt ggcatggcgg aagggctggt
     8161 gaactacacc cgtcttgatg atagcgcgga gaaaattatc catacccagg gttacccaat
     8221 gtgtccggac gatgaagtgt gggttgccga tgccgaagga aatccactgc cgcaagggga
     8281 agttggacgc ctgatgacgc gcgggccgta caccttccgt ggctattaca aaagtccgca
     8341 gcacaatgcc agtgcctttg atgccaacgg tttttactgt tccggcgatc tgatctctat
     8401 tgatccagag ggttacatca ccgtgcaggg gcgcgagaaa gatcagatca accgtggcgg
     8461 cgagaagatc gctgccgaag atatcgaaaa cctgctgctg agccatccgg cagtgatcta
     8521 tgccgcactg gtgagcatgg aaaatgagct gatgggcgaa aaaagctgtg cttatctggt
     8581 ggtaaaagag ccgctgcgcg cggtgcaggt gcgtcgtttc ctgcgtgaac agggtattgc
     8641 cgaatttaaa ttaccggatc gcgtggagtg tgtggattca cttccgctga cggcggtcgg
     8701 gaaagtcgat aaaaaacaat tacgtcagtg gctggcgtca cgcgcatcag cctgaaggag
     8761 agaacacgat ggctattcca aaattacagg cttacgcact gccggagtct cacgatattc
     8821 cgcagaataa agttgactgg gcctttgaac cgcaacgtgc cgcgttgtta atccatgata
     8881 tgcaggacta ttttgtcagc ttctggggcg agaactgccc gatgatggag caggtgatcg
     8941 cgaatattgc tgcgctgcgc gactactgca aacagcacaa tatcccggtt tattacaccg
     9001 cccagccgaa agagcagagc gatgaagatc gggcgctgtt gaatgatatg tgggggccgg
     9061 gcctgacccg ctcgccggaa cagcaaaagg tggtggatcg cctgacgcca gatgccgacg
     9121 acacggtgct ggtgaagtgg cgctacagcg cgtttcatcg ttctccgctg gagcaaatgc
     9181 tcaaagagag tggacgtaac cagctgatta ttacaggagt atatgcccac attggctgta
     9241 tgaccaccgc aaccgacgca tttatgcgcg atattaaacc gtttatggtg gcggatgcgc
     9301 tggctgattt cagccgtgac gagcatttga tgtcgctgaa atatgtggcc ggacgttctg
     9361 gccgggtggt gatgaccgaa gaattactgc cagcacctat ccccgccagc aaagcggcgc
     9421 tgcgtgaggt gatcctgccg ttgctggacg agtccgatga accgttcgat gacgacaacc
     9481 tgatcgacta cggtctggat tcggtgcgca tgatggcgct ggcggcgcgc tggcgcaaag
     9541 tgcatggtga tatcgacttt gtcatgctgg cgaaaaaccc gaccatcgac gcctggtgga
     9601 agctactctc ccgcgaggtg aaataatgga tttcagcggt aaaaatgtct gggtaaccgg
     9661 cgcaggtaaa ggtatcggct acgccacggc gctgacgttt gttgaggcgg gagcgaaagt
     9721 tacaggtttt gatcaagcgt tcactcagga gcaatatccc tttgcgaccg aagtaatgga
     9781 tgttgccgac gccgggcagg tggcgcaggt atgtcagcga ctgttagctg aaacggagcg
     9841 actggacgtg ctgatcaatg cggcgggaat tttgcgcatg ggcgcgaccg atcagctcag
     9901 taaagaggac tggcagcaga cttttgcggt taacgtcggc ggtgcgttta acctgttcca
     9961 gcaaaccatg aaccagtttc gccgtcagcg gggcggggcg attgtcactg tggcgtccga
    10021 cgccgcacac acggcgcgta ttggtatgag tgcttatggc gcatcgaaag cggcgctgaa
    10081 aagcctggcg ttgagcgtcg ggctggaact ggcgggtagc ggcgtgcgct gtaatgtggt
    10141 ttcgcctggc tccaccgaca ccgatatgca acgcacgctg tgggtgagcg atgacgccga
    10201 agaacagcgt attcgcggct ttggcgagca gtttaaactc ggcattccgc tggggaaaat
    10261 cgcccgtcca caagagatcg ccaacacgat tttgttcctc gcctctgacc tcgccagcca
    10321 tatcacccta caggatattg tggtcgatgg cggctcaacg ctgggggcat aagcatgatc
    10381 tggaaacgcc atttaacgct cgacgaactg aacgccacca gcgataacac aatggtggcg
    10441 catctgggaa ttgtgtatac ccttctggga gatgatgtgc tggaagccga aatgccggtt
    10501 gatacccgta ctcatcagcc gtttggcctg ctgcatggcg gcgcgtcggc ggcgctggct
    10561 gaaacgctgg gatcgatggc cggatttatg atgacccgtg acgggcagtg tgtggtgggc
    10621 acggaactta acgccaccca tcatcgcccg gtgtctgaag gcaaggtacg cggcgtctgc
    10681 cagccgctgc atcttgggag gcaaaatcag agttgggaaa tcgtcgtttt cgatgaacag
    10741 gggcggcgtt gctgcacttg tcggctgggt acggcagttt tgggatgagc ccgacagaat
    10801 taaatgagat tgcaggaaaa ctcggttaac ggagtgatcg agttaacatt gttaagttaa
    10861 atattggttt caactccgat ttacatggtt accgtgttgt taaatcgtac aaagatgtta
    10921 tagaaacaaa atgtaacatc tctatggaca cgcacacgga taacaactat gaacaaatca
    10981 gggaaatacc tcgtctggac agtgctctct gtaatgggag catttgctct gggatacatt
    11041 gctttaaatc gtggggaaca gatcaacgcg ctgtggattg tggtggcgtc ggtctgtatc
    11101 tatctgatcg cttaccgttt ttatgggctg tatatcgcca aaaatgtgct ggcggttgac
    11161 ccgacgcgta tgacgccagc ggtgcgccat aacgacgggc tggactatgt gccgacggac
    11221 aagaaagtgc tgttcggtca ccattttgcg gccattgccg gagcagggcc gctggtgggg
    11281 ccggtgctgg cagcgcaaat gggctacctg ccggggatga tctggctgct cgccggggtg
    11341 gttctcgccg gtgcggtgca ggatttcatg gtgctgtttg tttctacgcg ccgtgacggt
    11401 cgctcgctgg gtgagctggt caaagaagag atggggccaa ctgccggggt gattgcgctg
    11461 gtagcctgct ttatgatcat ggtcattatc cttgcagtac tggcgatgat cgtggtgaaa
    11521 gccctgactc atagcccgtg gggaacgtat accgttgcgt tcaccattcc gctggcgatt
    11581 tttatgggaa tctacctgcg ctatctgcgt ccgggccgca ttggcgaagt gtcagtgatt
    11641 ggcctggtgt tcctgatttt cgccattatc tctggcggct gggtggcaga aagtccgacc
    11701 tgggcaccgt actttgactt taccggcgtg cagctgacct ggatgctggt gggctacggt
    11761 tttgtggcgg cggtgctgcc ggtgtggttg ctgctggccc cgcgtgacta tctctcgacc
    11821 ttcctgaaaa tcgggactat cgtcggtctg gcggtaggca ttttgattat gcgcccgacg
    11881 ctgaccatgc ctgcgctgac caaatttgtc gatggcactg gcccggtatg gaccggtaac
    11941 ctgttcccgt tcctgtttat caccatcgcc tgtggtgcgg tgtctggctt ccatgcgctg
    12001 atctcttccg gcaccacgcc gaagatgctg gcgaacgaag ggcaggcgtg ctttatcggc
    12061 tacggtggga tgttgatgga atccttcgtg gcgattatgg cgctagtttc tgcctgtatc
    12121 atcgatccgg gcgtgtattt tgccatgaac agcccaatgg cggtgctggc tccggcaggg
    12181 acggcggatg tagtcgcttc tgccgcgcag gtggtgagta gctggggctt tagcattaca
    12241 ccagatacgc tcaaccagat tgccagcgaa gtgggtgaac agtcgatcat ttcccgtgcg
    12301 ggcggtgcgc cgacgttggc ggtggggatg gcttacatcc tgcacggtgc gctgggtggt
    12361 atgatggatg tggcgttctg gtatcacttc gccattctgt ttgaagctct gtttattctg
    12421 acggcggtag atgcgggtac gcgtgctgcg cgctttatgt tgcaggatct gctgggcgtg
    12481 gtgtctcctg gcctgaagcg gaccgattca ctgcctgcta acctgctggc aacagcgctg
    12541 tgcgtactgg cgtggggcta cttcctccat cagagcgtgg tcgatccgct gggcggtatt
    12601 aacactctgt ggccgctgtt tggtattgcc aaccagatgc tggcagggat ggcgctgatg
    12661 ctctgtgccg tggtgttgtt caagatgaaa cgtcaacgtt acgcctgggt ggcgctggta
    12721 ccaacggcct ggctgctgat ttgtaccctg accgctgact ggctgaaagc gtttagcccg
    12781 gatgcgaaag tcggcttcct ggtcattgct aacaagttcc aggcaatgat cgacagcggc
    12841 aatattccgt cgcagtatac tgagtcacag ctggcgcaac tggtgttcaa caaccgtctg
    12901 gatgccgggt taaccatctt ctttatggtg gtcgtggtgg tcctggcact gttctcgatt
    12961 aagacggcac ttgcggcatt gaaagatccg aagccaacgg cgaaagaaac gccgtatgag
    13021 ccgatgccgg aaaatgtcga ggagatcgta gcgcaggcga aaggcgcgca ctaaagtcag
    13081 agtgaggggg gatgttggcg aatgttggca tagtgccgag ggttccccct caccctaacc
    13141 ctctccccga tggggcgagg ggactgaccg agtgcgttga tagcatttgt aggccggata
    13201 aagcgttcac gccgcatccg gcactctttc agcaacatgg ttagcggagg ccaagatgtt
    13261 tgattcactg gcaaaagccg gaaaatattt aggtcaggcg gcgaagctga tgattggtat
    13321 gcctgattac gacaactatg tcgaacatat gcgggttaat catcccgatc aaacgccgat
    13381 gacctacgag gagtttttcc gtgagcggca ggacgcgcgc tacggtggaa aaggcggcgc
    13441 gcgctgctgc taaatttgca cgtcaggctt taaacgattc cactttttcg aacgctgcac
    13501 gcaacgtatc tggtgtcagc gtgactggca ggtaatgaat ggactccacc gggcgcaagg
    13561 tgtgggcaat cactttgtcg atctccgcct gattattgat atccacctcc agctccgcca
    13621 gcgtagtcgg cagatgaaaa cgctgatacg ccccggttaa ttgcgccagc acatcatcct
    13681 gacccagcaa ggcgctttgc accagaattc cgtaagcgac tttggtgccg tgaagaaact
    13741 tctcggtttg cggcaggaca gtcagaccgt tatgcacggc atgagccgcc gccacacgcg
    13801 taaaacgatc gcccagacca ccgaccatcc caccaccagc aataatagca tccaccacat
    13861 cgcaaaatga ttgcgttaac tgctgatttt gctgatcggc aagtgcctgt tcgctactgt
    13921 ttaacaagac gtcgcgaatg gcctgcgcat tattgatccc cagccgcacg gttagcggca
    13981 acgtttctgg ttgcggagcc agcaccaccg cttcatacca tttcgccagc gtgtcaccga
    14041 tccccgccag cagatattgt tgcggtgcat tgaggataat ctccggttcc accagcacca
    14101 taaaattggc gtcgtcgaaa atctcataat gcagcgcctg tccggcatcg ttataccaga
    14161 cggagagcgg tgtccaggct gcgca
//
LOCUS       DAJTYH010000093        14028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTYH010000093 DAJTYH010000000
VERSION     DAJTYH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..178)
                     /locus_tag="OO609_003008"
     CDS             complement(<1..178)
                     /locus_tag="OO609_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6634344.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQL"
     gene            452..3976
                     /gene="nifJ"
                     /locus_tag="OO609_003009"
     CDS             452..3976
                     /gene="nifJ"
                     /locus_tag="OO609_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6634345.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4343..5472
                     /gene="ompN"
                     /locus_tag="OO609_003010"
                     /pseudo
     CDS             4343..5472
                     /gene="ompN"
                     /locus_tag="OO609_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5613..6047
                     /gene="uspF"
                     /locus_tag="OO609_003011"
     CDS             5613..6047
                     /gene="uspF"
                     /locus_tag="OO609_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6634346.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6223..7158
                     /gene="ttcA"
                     /locus_tag="OO609_003012"
     CDS             6223..7158
                     /gene="ttcA"
                     /locus_tag="OO609_003012"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6634347.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7287..8654)
                     /gene="dbpA"
                     /locus_tag="OO609_003013"
     CDS             complement(7287..8654)
                     /gene="dbpA"
                     /locus_tag="OO609_003013"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6634348.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9132..10115)
                     /gene="zntB"
                     /locus_tag="OO609_003014"
     CDS             complement(9132..10115)
                     /gene="zntB"
                     /locus_tag="OO609_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6634349.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10370..11602
                     /gene="dgcM"
                     /locus_tag="OO609_003015"
                     /pseudo
     CDS             10370..11602
                     /gene="dgcM"
                     /locus_tag="OO609_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11623..12186)
                     /gene="smrA"
                     /locus_tag="OO609_003016"
                     /pseudo
     CDS             complement(11623..12186)
                     /gene="smrA"
                     /locus_tag="OO609_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12440..13906)
                     /locus_tag="OO609_003017"
     CDS             complement(12440..13906)
                     /locus_tag="OO609_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6634350.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGIACGLAWNIQI
                     MILFRALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLT
                     DNLDWRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYT
                     LEEGARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFS
                     FSGGVGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRW
                     LLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKER
                     LKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSAL
                     FFNRSRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(13903..>14028)
                     /locus_tag="OO609_003018"
     CDS             complement(13903..>14028)
                     /locus_tag="OO609_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6634351.1"
                     /translation="ATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3745 a         3531 c         3316 g         3436 t
ORIGIN      
        1 ccagttgacg ggcaacagaa agcgaaccgc cgatcattgc acaatttgct tctccactac
       61 tggacatcga cgcttttaaa cttggcgcta cgtgcgccgc agtcgcctgc tgaacaggct
      121 cactactaca cgccgacaac aataaagcgg cacaccctac ccaaaacgct gctcgcatct
      181 cttcttcctc tgatcttcaa gccaaacgac accgccataa ataataggca gcacagaggg
      241 cggcgtcgag agctgtcctg cgcgttgccc cgcattttta cttttttatg gctatttttt
      301 tgccctctgt ttgatcaaaa cattcattac tctgatgtgg gggacacaaa agcgaaaatg
      361 cagaagaaag ccatttgcta aaattgaagg attactactg ggcgcgcagc aatttcgtgc
      421 gcccctcatt tgcgcaatgt aagggtgtca tatgattact attgacggta atggcgcggt
      481 tgcttcggtg acgtttcgca tcagtgaagt tatcgccatc taccctatta cccccagttc
      541 cacgatggca gaacaggctg atgcctgggc cggaaacggc ttgaagaacg tttggggaga
      601 cacaccacgc gtggttgaaa tgcagtcgga agcgggtgct atcgctaccg tgcatggcgc
      661 tttgcagacg ggtgcccttt caacatcgtt tacgtcatcg cagggtttgc tgctgatgat
      721 cccgacgctg tacaaactgg caggcgaact gacgccattt gtcctgcatg tagcggcacg
      781 taccgttgcc acacatgcac tttctatttt tggcgatcat tccgacgtta tggcggtgcg
      841 ccagacgggt tgcgcgatgt tgtgtgcggc taacgtccag gaagcgcaag actttgctct
      901 catttcgcac atcgcaacgc tgaagagccg cgtgccattt attcatttct ttgatggttt
      961 tcgcacgtcc cacgaaatca ataaaattgt cccgctggcc gatgacacta ttcttgatct
     1021 catgccgcag gccgaaattg atgctcatcg cgcccgggcg ctcaacccgg aacatccggt
     1081 gatccgcggt acgtccgcca atcctgacac ttatttccag tctcgcgaag ccaccaatcc
     1141 ctggtacaac gcggtctatg accatgttga acaggcgatg aatgatttcg ctgccgcgac
     1201 aggtcgtcag tatcagccgt ttgagtatta cgggcatccg caagcggaac gggtgattat
     1261 cctgatgggc tctgccattg gcacctgtga agaagtggtt gatgaattgc taacccgtgg
     1321 cgaaaaagtt ggcgtgctga aagttcgtct gtaccgtccc ttctccgcca aacacttgct
     1381 gcaagctctg ccgggatccg tacgcagcgt ggcggtactg gacagaacca aagaacccgg
     1441 tgcccaggca gaaccgctct atctggatgt gatgaccgca ctggcagaag cctttaataa
     1501 tggtgagcgc gaaaccctgc cccgtgtcat tggtgggcgc tatggtcttt catccaaaga
     1561 atttggcccg gactgtgtac tggcggtatt tgccgagctc aacgcggcta aacctaaagc
     1621 acgctttacg gttggtattt acgatgatgt gaccaatctg tcactgccgt tgccggaaaa
     1681 cactctgcca aactcggcga aacttgaagc cttgttttat ggccttggta gtgatggcag
     1741 cgtttccgca accaaaaaca atatcaagat tatcggtaat tccacgccgt ggtacgcaca
     1801 gggctatttt gtttacgact ccaaaaaagc gggcggcctg acggtttctc accttcgcgt
     1861 gagcgaacag ccgattcgtt ccgcttatct catttcccag gctgattttg ttggctgcca
     1921 ccagttgcag tttatcgata aatatcagat ggctgagcgt ttaaaaccta gcggcatttt
     1981 cctgcttaat acgccgtaca gcgcagatga agtgtggtcg cgcttgccgc aggaagttca
     2041 ggccgtgtta aaccagaaaa aagcgcgctt ctatgtgatt aacgcggcga aaatcgcccg
     2101 cgaatgtggc ctggcggccc gtattaatac cgtcatgcag atggcttttt tccatctgac
     2161 gcaaattctg cctggcgata gcgccctcgc agaattgcag ggtgcgattg ccaaaagtta
     2221 cagtagcaaa ggtcaggatc tggtggagcg caactggcag gctctggcgc tggcgcgtga
     2281 atccgtagaa gaagttccgt tgcaaccggt aaatccgcac agcgccaatc gaccgccagt
     2341 ggtttccgat gccgcccctg atttcgtgaa aaccgtaacc gcggcgatgc tcgccgggct
     2401 tggtgatgcc ctccccgttt cggcgctgcc gccagacggc acctggccga tgggcaccac
     2461 tcgctgggaa aaacgcaata tcgccgaaga gatccccatc tggaaagagg aactctgtac
     2521 ccaatgtaac cactgcgtcg ccgcttgccc acactcagct attcgcgcaa aagtggtacc
     2581 gcctgaagcg atggaaaacg cccctgccag cctgcattcg ctagatgtga aatcgcgtga
     2641 tatgcgcggg cagaaatatg tcttgcaggt ggcaccggaa gattgcaccg gttgtaacct
     2701 gtgcgtcgaa gtttgcccgg cgaaagaccg tcagaatcca gagattaaag ccatcaatat
     2761 gatgtctcgc caggaacatg tcgaagaaga gaaaatcaat tacgatttct tcctcaacct
     2821 gccagaaatc gaccgtagca aactggaacg tattgatatt cgtacatcgc agctgattac
     2881 accgctgttt gaatattcag gtgcttgccc cggttgtggc gagacgccgt atattaaatt
     2941 gctgactcag ctctatggtg accggatgtt gatcgctaac gccaccggct gttcttcaat
     3001 ttatggcggt aacctgccct ctacaccgta taccaccgat gccaacggtc gtgggccggc
     3061 atgggcgaac tctctatttg aagataatgc cgaatttggc cttggtttcc gcctgacggt
     3121 cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa tttgccgata aaatcccggc
     3181 ggaattactg acggcgttga aatcagacgc tacgccagag gttcgtcgag aacaggtcgc
     3241 agctttacgc cagcaactca acgatgttgc cgaagcacat gaacttctgc gtgacgcaga
     3301 tgcactggtg gaaaaatcaa tctggctgat tggtggcgac ggctgggctt acgatatcgg
     3361 ctttggcggt ctggatcatg tattgagttt gacggaaaac gtcaacattc tggtgctgga
     3421 tacgcaatgc tattccaaca ccggtggtca ggcgtcgaaa gcgacaccgc tgggtgcagt
     3481 aactaagttc ggcgagcacg gcaaacgtaa agcgcgtaaa gatcttggcg tcagtatgat
     3541 gatgtacggt catgtttatg tggcgcagat ttctctcggc gcgcagctga accagacggt
     3601 gaaagcgatt caggaagcgg aagcgtatcc gggaccatcg ctgatcattg cttatagccc
     3661 gtgtgaagag catggttacg atctggctct cagccacgac cagatgcgcc aactcactgc
     3721 taccggcttc tggccgctat atcgctttga tccgcgtcgt gccgatgaag gcaaactgcc
     3781 gctggcgctg gattcacgcc cgccgtcaga agcactggaa gaaacgttac ttcacgagca
     3841 gcgtttccgt cgtctgaatt cgcagcagcc agaagtggca gaacagttat ggaaagatgc
     3901 tgcagctaat ttgcaaaaac gctatgactt cctagcacaa atggccggaa aagcggaaaa
     3961 aagcaacacc gattaaatgc tctggataag gattatccaa ttctaaaaaa aaagcccgga
     4021 cgactgttcg ggcttgtctt tttatatgtt ggaaaatcag tggcaatgca atggcccaac
     4081 agaaaataaa atctgtgaca ataaaggcat ataacccgcg cagaataacg tataggaaat
     4141 taaaagtata atttttattt tgtatgtatt atcttcgata cattcaccag acttattatc
     4201 atttcagagg aattatttga ttaaggtttt acttaaggcg taacaaatga tatttatcgg
     4261 ctaactgaac ttctccttta ggatgttttc actccccgaa atggggataa aaggcaaata
     4321 aaataactaa aggatttatt caatgaaaag aaaagtactg gcacttttaa ttcctgccct
     4381 gctcgccgca ggtgctgcac atgcagccga agtttataat aaagacggca acaaattaga
     4441 tctgtatggc aaagttgatg gcctgcatta tttttctgat aattcagcga aagatggcga
     4501 ccagagctat gcgcgtctgg gttttaaagg cgaaacccaa attaacgatc aacttactgg
     4561 ctacggtcaa tgggaataca atattcaggc aaacaacact gaatcttcaa aaaaccagtc
     4621 atggacccgt ctggcatttg ccggactgaa atttgcagat tacggttctt tcgattacgg
     4681 acgtaattat ggcgtaatgt acgacatcga aggctggacc gatatgctgc ctgaatttgg
     4741 cggtgactct tataccaatg cagacaactt tatgactggt cgagccaatg gcgtcgcgac
     4801 ttatcgtaat actgatttct tcggtctggt aaatggtctg aacttcgcgg tgcagtatca
     4861 aggtaacaac gaaggagcca gtaatggtca ggaaggcacc aataacgaac gcgatgttcg
     4921 tcatgaaaac ggtgacggct ggggtctttc cacaacatat gatttaggca tgggctttag
     4981 cgctggtgcg gcatacacct cttctgaccg caccaatgac caggttaacc atactgcggc
     5041 gggtggtgat aaagcagacg cgtggactgc tgggctaaaa tacgatgcta acaatattta
     5101 cctagcaacc atgtattcag aaacgcgtaa tatgaccccg tttggcgaca gcgattatgc
     5161 agttgcgaac aaaacccaga actttgaagt tactagtacc agtttgattt ttgtctgcgt
     5221 ccggcagtct ctttcctgat gtctaaaggc cgtgaccttc acgctgctgg tggtgcagac
     5281 aacccggcag gtgttgatga taaagatctg gttaaatacg ccgatgttgg cgcgacttac
     5341 tatttcaata aaaacatgtc cacctatgtt gactataaaa tcaacctgtt ggatgaagat
     5401 gacagtttct acgctgccaa cggcatctct accgatgata ttgtcgcttt aggtctggtt
     5461 tatcagttct aaatcctcct gcccgctgtt atggcgggct ttttctgctt attcttcctc
     5521 ctttgatcta aattaaaaat gtgaactccg tcattacaca aaaagtgtca tctggcgtta
     5581 cactttatgc ggatactaaa acaggaggtt ttatgaacag aacgattctt gtccctatcg
     5641 atatttcaga ttcagaatta actcaacgcg tgattagcca cgttgaggca gaggcaaaga
     5701 ttgatgatgc agaggttcat ttcctgacgg taataccttc actgccctac tatgcctctc
     5761 tgggtttagc gtattccgca gaattaccgg caatggatga cctgaaagcg gaagccaaat
     5821 cgcaactgga agagatcatt aaaaaattta aactgccaac cgacagagtg catgtccatg
     5881 ttgaggaagg ctcgcccaaa gaccgcattc tggaattggc gaagaagatc cccgctcata
     5941 tgatcatcat tgcttcccat cgaccggata tcaccactta tctgctcggt tccaacgccg
     6001 cagctgtagt gcgtcacgca gagtgctccg tgctggttgt gcgctgacac taacgcccgc
     6061 acattgctgc gggctttttg attcatttcg caaatgtgct gacattttcc cctctaatcc
     6121 gtaccataca cgccacagtt tttatatcag atttcttatg ctgagcgttc cggcatgacg
     6181 catactcttc tgatgccata taacgaattg agtcgctttt aaatgtcgca aaatcaagaa
     6241 attagtaaga aagaacaata caacctgaac aaattacaaa aacgtctgcg tcgtaacgtg
     6301 ggcgaagcca ttgctgactt caatatgatt gaagaaggcg atcgcatcat ggtttgcctc
     6361 tccgggggta aagacagcta taccatgctg gagattctgc gcaatttgca gcaaagcgcg
     6421 ccaatcaatt tttcgctggt ggctgttaac ctcgatcaaa agcaaccggg cttcccggaa
     6481 cacgttctgc ccgagtatct tgaaaagctg ggcgttgagt acaagattgt tgaagagaac
     6541 acttacggta tcgtgaaaga gaagattcca gagggcaaaa ccacttgctc actgtgttct
     6601 cggctacgtc gcggtatcct ttatcgcacc gcaacggaac tggggacgac gaagatcgcg
     6661 ttgggtcacc atcgtgacga tatcctgcaa acgttgttct taaatatgtt ctacggcggc
     6721 aagatgaaag gtatgcctcc gaaactgatg agcgatgatg gcaaacatat cgttattcgt
     6781 ccactggcct actgccgcga gaaagatatt cagcgatttg ccgatgcaaa agcgttcccg
     6841 attattccgt gcaacctgtg cggttcacag cctaacctgc aacgtcaggt gattgctgac
     6901 atgttgcgtg actgggataa acgttatcca gggcgtatcg agacgatgtt cagcgcgatg
     6961 cagaatgtgg tgccgtcgca tctgtgcgat accaacctgt tcgatttcaa aggcattacc
     7021 cacggttctg aagtggttaa cgggggtgat ctggcgtttg atcgcgaaga gatcccacta
     7081 caaccggcgg gctggcagcc agaagaagat gaaaatcagt tggatgagtt acggctgaat
     7141 gtggttgaag tgaaataacc aggatagcgc ccgatgcgca agcgtatcgg gctactctta
     7201 tggaggccgg ataagacgcg gccagcgtcg catctggcaa tcccgaataa gatgtttact
     7261 cttgcacccg gcaattcaac atttcattat tttaataacc gcacccggca cgtttttcct
     7321 ttaatcttcc cgccctgtaa ctgtttccat gctttatgag caacagcctg acggaccgcg
     7381 acatagacat gcgccggatg cacggcgatt ttgccaatat ctgcgccatc aagcccgata
     7441 tctcctgtca gtgcacctaa tacatcaccc gggcgcattt tggctttttt cccgccatcg
     7501 atacacaacg ttgccatttc tgcttccagc gtcacaatgg aactattagc tggcggcgtt
     7561 tgccagttaa gttttatctg caacatgtca gaaatgatat tggcccgctg tgcttcttcc
     7621 ggagcacaga aactgatcgc cagaccgcta tttcctgcac gagctgtacg accgatgcga
     7681 tgtacatgaa cttcagggtc ccacgccagc tcaaagttca ccaccagctc aagcgattta
     7741 atatccagac cacgcgcagc aacatcagtc gcaaccagta cacgggcgct accgttagca
     7801 aaacgtacca gggtctgatc gcgttgctcc agatcgccgt gtaacgacaa tgcactttgc
     7861 tctacttcat tcagcgcgtc gcagacagcc tggcaatctt ttttggtatt gcaaaacacc
     7921 acacaagagg atggctgatg caagcttaat aaccgttgca acagaggaat tttgccttta
     7981 ctggatgtct cataaaattg ttgttcaatg ggtggcaaag catctgttga gtcaatttca
     8041 atcgccaaag gatcgcgttg cactcgtccg ctgattgcgg cgatggcttc cggccaggtt
     8101 gccgaaaaca gaagcgtctg tcgagatgca ggcgcaaaac ggatgacatc atcaatggca
     8161 tcgctaaatc ccatatccag catgcggtcg gcctcatcca tcaccaaagt attcagcgca
     8221 tccagtgata ccgtgccttt ttgcaagtga tccagcaaac gacccggcgt tgccacgata
     8281 atatgcggcg catgttgcag cgaatcacgc tgcataccga acggttgacc accgcacaac
     8341 gtcaaaattt tggtatttgg cagaaaacgc gccagccgac gcaattcacc tgccacctga
     8401 tccgccagtt cacgcgtagg acacagcact aaagcctggg tttgaaatag cgacgcatca
     8461 atttgctgta acaagccgag gccaaaagcc gccgttttgc cgctgccggt tttcgcctgc
     8521 acgcgaacat cttttccggc aaggatcgcc ggaagcgcgg cggcctgcac cggcgtcatg
     8581 gttaaataac ccaactcatt aaggttcgtg agttgggcgg gaggcaaaac attcagggta
     8641 gaaaaagcgg tcacaatcta ttctcgtggt catcgacgca aagttagcag gcgcgtatcc
     8701 tcgcagatct acgctcacga tgcgacaatt taatcggttc ttcatcgggt ggtgggtcag
     8761 gcatgggttg cgggcgaggg atcggatcgg gcactggaac aggatcgcca ggaatcggtt
     8821 cagggacagg aatttgcaaa taaataagtg tcgtcatatt tccctccggt cattgggtgg
     8881 actcttaaag ggtagacgct gataaataac aggcaaaaaa aagccgactc atcaaagtcg
     8941 gcgtcgtacg aatcaattgt gctatgcagt aattcaaaaa aggaagtaag acaatatgga
     9001 gcgcaacgcc catcgcttga cgttgcattc acctgcaagg gagatattgc cctgaatggg
     9061 tagagagttt attgacttcg ctcaaacttt gcggcgtttt tgtatacaga cagccggaaa
     9121 aattgctttt tttacaacca tttactacgg tgcaaccata aagcaacacc accaataaga
     9181 acaactaaca gaatacaaaa aattgaaaat ccgaattgcc acccgccgcc agggatccca
     9241 ccaaggttga cgccaaataa cccggtcaga aaggtactgg gtaaaaagac cattgccatc
     9301 aacgacattg tataggtacg acgagctaaa ttttcctgca tcacctgagc gatttcatcc
     9361 gccatcacgc cagtccgtgc tatacaggcg tcgatttcgt caaggccgcg cccaaggcga
     9421 tcggcaatat cctgcatccg acggcgttgg tcatcgctca tccacggcaa acgttcactg
     9481 gcaagacgag cataaacatc acgttgcggt gccatatagc gacgcatcac aattaattgt
     9541 ttgcgcagca gagccaggaa tccacgcggt ggaatttgct gatcaaggag attatcttca
     9601 aggtcgataa ttttatcgtg cagctgctcg ataaattcac tggaatgatc ggtcaacgca
     9661 tcgcacacat ccaccagcca tcccccgcaa tcggtcggac ccgtgccctc ttccagatcg
     9721 ctcaccacat cgtccagcgc cagcactttg cgttgtcggg tcgaaacaat taaccgcccg
     9781 tccatatata cacgcatggc gaccagttga tcggggcgtt catcggtgct gccgtttata
     9841 cagcgcaatg taatcagcgt gccttcaccg aaacggctga ctcggggccg tgtgctctcg
     9901 cccgccagcg catcacgtac gttattggga agcagcggtg ttgtcgccag ccattgggcg
     9961 ctatcatggt gtacataatt aaggtggagc cagcagggat gcgcttcatc aatcacatct
    10021 gtattttcca gcggtttaac gccgcctcta ccatccagca tccaggcaaa tactgcatcc
    10081 gggacattaa cgtccgatcc cttaatcgct tccactgtgc ctccatcatc aacgcattat
    10141 tttgtagtct agccttctgg ccctgttacg caacatctca tcaccccatt accctgaaat
    10201 gattaataaa attctgtcta aattgaatac aaaaagcaaa atgcttttcc gtatacaaac
    10261 cgtgtgaagt gttaaatagc gtctatcatt atcagaatta tctgatcata tgacgtggct
    10321 tttttgcgat cggatagcaa caaaaattga taaaaataac gggatctcaa tgattacgca
    10381 caacttcaat accctggact tactcaccag tcctgtctgg attgtttcgc cctttgagga
    10441 acagttaatt tatgccaata gcgcggcgcg actgttgatg caagacctca cgtttagtca
    10501 gctacgaacc ggaccctatt ccgtctcctc acaaaaagaa ctgccgaaat acctctccga
    10561 tctgcaaaac caacacgata ttatcgaaat cctcactgtt cagcgtaaag aagaggaaac
    10621 agcattaagc tgtcggcttg ttttgcgaga gctgacagaa acagaaccgg tgattatttt
    10681 cgaaggtatc gaagcgccgg caacgctggg tttaaaagcc agtcgctcgg caaattatca
    10741 gcgcaaaaaa caaggttttt atgcgcgctt ttttctgact aactctgcac caatgttgtt
    10801 gattgacccg tcacgagatg gacaaatcgt cgatgctaac ctcgccgcgc tcaatttcta
    10861 tggttataac catgaaacga tgtgccagaa acatacctag gaaataaata tgcttgggcg
    10921 tcgcgtcatg cctatcatgc atgaaatttc gcatttaccc ggcggtcata accctttgaa
    10981 ttttgttcat aaactggcgg atggatctac tcgtcatgtg cagacctatg ccggaccgat
    11041 tgaaatttat ggcgacaagc tcatgttatg tattgtgcat gatattactg agcaaaaacg
    11101 gctggaggag cagctggaac atgctgctca ccatgacgcg atgaccggat tactgaatcg
    11161 gcgacagttt tatcacatta cggaaccagg ccaaatgcag catctcgcca tcgctcagga
    11221 ttacagcttg ttgctcatcg acaccgatcg ttttaaacac attaacgatc tctatgggca
    11281 ttctaaaggt gatgaggtgt tatgcgccct cgcccgcccc ctcgaaagtt gcgctcgcaa
    11341 aggcgatttg gtgtttcgtt ggggaggcga agagtttgtc ttattgctac caagaacccc
    11401 actggatacc gcgctttcgc tggctgaaac tatccgcgta agcgtggcaa aagtgagtat
    11461 ttcgggctta ccacgcttta ccgtcagcat tggtgtggcg catcacgaag gaaatgaaag
    11521 catcgatgaa ctgtttaaac gcgttgatga tgctttgtat cgggcgaaaa atgatggacg
    11581 caaccgcgtg ctggcggcat aagccgcgga tgcgtctcga gatcaacgac tgcgcttagc
    11641 gtggcgctcc cagttttctt gcttcgcctg cgccgtttta cgtagtgcga cgtaacacgc
    11701 cccgctgccg ccatgatgcg gtagcgcggt gcaatatgcc tgaacatcat caaattcggt
    11761 cagccagcgc gccacatagc tgcggacaat attggcatgt gatttatctt cccgcccttt
    11821 accatgaata atcagcacat tacgcaaacc atccgccagg gcttgttgaa tgaaactgaa
    11881 caccattttg cggcactctt ccaccggctg gcgcaaaaga ttcaggctcg cctgttgcgg
    11941 atatttacca ctgcgcagct tatccagcac cccatgctac aacccttcct gccgaaactc
    12001 cagcggctga cttagcggga tgatgtcgag aaatccggta gtgaggaaat tatcaagctg
    12061 cagcgtgtcg atacgctgcg gcgcacgttg gttacgcgtt ggatgccagt ggacatctgt
    12121 agcacgtttc agcggctgga catcttccat ggcgtcaaga aacagcgatt tgtcgtcaag
    12181 gttcatgtaa cctcctatgc accttcacac gtcgtgattc agacgggtta attgttcata
    12241 cttactgcat cgttcccttc acagattgaa acaatactct acttcatacc tgtgagtatc
    12301 tgcgcaggcg gggcatttta tactagcctg ttagcagtat cgaacagttt agcccgagtt
    12361 taattcagca caggctccga tcaggatgat ttattcatag caggaaccaa caaaaatgcg
    12421 ataaataaca gaccactgat tagcagaaag gtaccgaaaa tgccatagtt tgcgcctccc
    12481 tcagcatcaa ttgactcaat aatttggttg aagccagtat ttccgatgtc tggtcacttc
    12541 tactgcgatt aaaaaataaa gcacttctgg aaatgaagtc attcaccgta tggggaacag
    12601 aaaccatttt ttcacccatt ctcgaaaaat gaaaattagt tcgattattg agaatagagc
    12661 cacacagggc aataccaatc gcgcccccaa gattacgtgt taaattaaat acaccagagg
    12721 ctaacttcag ccgttctttt ggaattcccc ccagcgttaa tgttacgatg ggggccatcg
    12781 caaactgctg cgaaatccct ctaattgcct gtggaaacag taattcctgc catccccatt
    12841 cgtgggttat aggggtaaaa agatacattg aaaacacaaa tccgcccagc cccgccatca
    12901 acagccatcg cagatttatt tttttactta accagaagta aaagggaaca gaaaataact
    12961 ggaaaatacc tgttgtgcaa acggcaaaac ctatctcttc ggcatttaat ccgcgaatct
    13021 gccccaaaaa cacaggtatt aaatatacag tagagaatat cccaacccct ccagaaaaag
    13081 aaaaaaaaca tcccagagtg aaatttttat ccttaaaagc atgaagatcc attatcggat
    13141 tggatattgt aagtgtacgc gcagcaaata aaataaaact caccaatgcc agaactgatg
    13201 tcagcaaaat agtattatca tccagccatc cccagcgcgc tccttcttca agggtatatt
    13261 ccagacatcc cagagtcatg gccaacagga taatactcgg ataatcagca actttcagta
    13321 aagagagatc aggtttatca aaattaacca gaaaaggaat actcaaaact aaatatatgc
    13381 caggtaatat attgatataa aatagccatc gccagtcgag gttatcagtg agccagcccc
    13441 ccagagttgg ccctagcgtc ggtgacagtg aggccagcgc actgactacc gcagcagcca
    13501 accccaactc tttaccctgg taataaataa acgccatcgt gaaaactaaa gggatcatgg
    13561 atgcgccagc agctccctgt aaagcacgaa acaaaatcat gatctgaata ttccatgcaa
    13621 gtccacacgc gattccacac gcgatactca ttaaagtaaa tattcccgcg gatagagtaa
    13681 aaagccaacg agtggaaaat acccgtgaca gccacccaga gagcggaata acaataatct
    13741 cagcaattaa atagctggtc tgaagccagg ccatttcgtc tttacctgcg gacagaccac
    13801 cacctatttc atttagggat gacgatacaa tttgaatatc aagctgagca ataaataatc
    13861 ccatacacat actggcgaaa gcaatatgtt tacgcataga catcatctct catcaacact
    13921 gacggtgacg gacagtcccg ggcgaagaag ttgctttaat tcctttgcgt cgtcaaatgc
    13981 aatccgtacc gggacacgct gaacaatttt ggtgaaattg ccagtggc
//
LOCUS       DAJTYH010000094        13870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTYH010000094 DAJTYH010000000
VERSION     DAJTYH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..2615
                     /locus_tag="OO609_003019"
                     /pseudo
     CDS             1..2615
                     /locus_tag="OO609_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2625..6271
                     /locus_tag="OO609_003020"
                     /pseudo
     CDS             2625..6271
                     /locus_tag="OO609_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6332..6739
                     /locus_tag="OO609_003021"
     CDS             6332..6739
                     /locus_tag="OO609_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634352.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7281..8369
                     /locus_tag="OO609_003022"
     CDS             7281..8369
                     /locus_tag="OO609_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6634353.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8380..10659
                     /locus_tag="OO609_003023"
     CDS             8380..10659
                     /locus_tag="OO609_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634354.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10652..11788
                     /locus_tag="OO609_003024"
     CDS             10652..11788
                     /locus_tag="OO609_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6634355.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11785..13788
                     /locus_tag="OO609_003025"
     CDS             11785..13788
                     /locus_tag="OO609_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634356.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3416 a         3654 c         3564 g         3236 t
ORIGIN      
        1 cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg cattaacact
       61 gcttatacgt gtagccgggc atactaaacg ctgtcacgat cgaatgacga aagcctgcgc
      121 tgcgttccca cacgcagcac tggcagcact ggcggaactt ctagtgcaaa aagaagagaa
      181 tagttggcgc attatgctaa tgacgatgtt tatctcgcaa ccagcactgg cagaacaggt
      241 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
      301 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
      361 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
      421 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
      481 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
      541 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
      601 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
      661 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
      721 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
      781 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatcca caggaagcct
      841 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
      901 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
      961 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacag gataacgccc
     1021 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1081 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     1141 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     1201 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     1261 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     1321 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     1381 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     1441 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     1501 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     1561 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     1621 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     1681 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     1741 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     1801 tgaaagacct gccaaaacca aacaaaagcg attatgagtc acaggcgaac gatgctgtta
     1861 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc ggcccagcag gtagcaaggc
     1921 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     1981 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2041 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     2101 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     2161 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     2221 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     2281 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     2341 aaggctggat aaagggcgaa ccacaggatg gaggctggat gatcaaacct ctcggtcgct
     2401 ggtcgttaat catggaaatc gacgaaggtt ttgcggtggg tatatcacca gccgaactta
     2461 gcgctgagca gctcttaagc aagctgtggc tatgggaagg caaagcggaa agctatggct
     2521 gggggagtaa ttcaacacag gaagcgcagt tctccgtact cgatgccatc accgccagcg
     2581 agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac aaggaattac
     2641 cgtggctggc ggataacgcc caactggaac tgaaatataa aaaaggcaaa acaccactca
     2701 gtcatcgcaa ttggccgggc gaaccagtgc ctgttatcac tgaaagcatc attcagacac
     2761 tgggtgatga attgctacaa aaagctgaga agaaaaaaaa cattgtctgg cgttatgaaa
     2821 atttttcacc ggagtggcag tccgccatta cgcaggccat caacttgatc ggcgaacaca
     2881 aaccctcagt cccggcccgc acaatggcgg cactagcctt tatcgcgcaa aatgacaacc
     2941 aacagttgct cgacgaaatc gtccaacaag aggggctgga atatgcgact gaggtggtga
     3001 ttgcacgcca gtttattgca cggtgttatg agagtgatcc tctggtagtc acattgcagt
     3061 atcagaacga ggactatggc tatggttatc gctcagaaac ctataacgaa ttcgatctcc
     3121 gactgcgtaa acacctctct ctggcagagg aaagctgctg gcagcgttgc gccgacaaac
     3181 tcattgccgc actgccagga ataaccaaag ttcgccgccc ttttattgcg ctgatcctcc
     3241 ccgaaaaacc agaaatcgcc aatgagttgg taagccttga atgcccgcgg actcattttc
     3301 attctaagga gtggttaaaa gttgttgcta ctgaccccaa agcggtgaga aaactcgaac
     3361 gctactggag ccaggatata tttagcgatc gagaagccag ctacatgtcg catgaaaacc
     3421 acttcggcta cgcggcctgc gctgcccttt tgcgcgaaca aggactggca gtcgttccgc
     3481 gcctcgcgat atatgcccat aaagaagatt gcggcagtct gctggtacaa attaaccatc
     3541 cgcaagtcat ccgcaccttg ctgttagttg ctgataaaaa caaacccagc ctgcaacgtg
     3601 tggctaaata cagtaaaaac ttcccccatg cgacactcgc cgcactggca gaactgctgg
     3661 cgttaaaaga accaccagcc cgcccaggtt atccaatcat cgaagacaaa aagctgcctg
     3721 cacagcaaaa agctcgagat gaatactggc gtacgctgtt acaaacgctg atggcatcgc
     3781 agccacaact ggcagcagaa gtgatgccgt ggttaagtac tcaagcccgg gcagtgctga
     3841 atagttattt atcggcaccg cccaaaccag ttattgatag caccgataac agccaaatgc
     3901 ctgaaatcct cgtttcacca ccatggcgtg ggagaaagaa aaagacttcc atacgtcact
     3961 tttcgctgcc tgatcttacg cttgcaccgc aaacgtacag gtcgccgaat aaacactgga
     4021 cgccggaaca gcaaactgca attgaccatt ttagcgccat gtcttttgac gaacgtcttg
     4081 ctacgagagg agcggatatc ttcttgcggg aattaggttt tgacgttcat agcctggaat
     4141 ttaataagcg tcttcttaat ggacagttgg atgcctttga tgctcagcaa ctgcaagaat
     4201 acacatcaat aggactattg cgtacttatc acgcgcatca ggccaaatct aaacagtacc
     4261 gggaagacgc caccaatgca ttactggcac aagatagcat tgcgttgatg ggagggtggc
     4321 gaacttttca gcacggattt tactatggta atgaacagcg ttgggggatt tggaatctgt
     4381 accttatcgc gcagatgccg cgcgaaatgg cggtatcgtg ctggcaacaa attgtcgagg
     4441 ctgattttca ttacaccggg gtggaatatt tgttaagtgt gctgggtacc gatgctttgc
     4501 ctggattaaa caccgccttt gcccgacatc caaaagagat atttccgctg ctgattcatt
     4561 tcggcgcaac agaactggcc ctgcccattg cccgcgtctg gcaccttttt gcgggccagc
     4621 gtaatctggc tcgccagtgg attttacaat ggccggaaca tacggctact gcacttatcc
     4681 ctcttgtctt caccaaatct agcgataaaa gcgaagctgc attacttgcc ctgcgtttac
     4741 tttacgaaca tgggcatggc gaatggctac aaactgtagc caatcgttgg cagcgtacag
     4801 atctatggcc tgccctggaa caattactta aacagagtcc aatagagatt tacccggcac
     4861 gcattccaaa agcccctgat ttctggcatc cgcaaatgtg gtccaggccg cgccttatca
     4921 ctaataatca acctgttacc gatgacgctc tggaaattat cggcgaaatg ctgcgcttta
     4981 cccagggggg acgtttttat agcgggctgg aacaactgaa aacgttctgc cagccacaaa
     5041 cgctggcagc ttttgcctgg gatctcttca ctgcgtggca acaagctggt gcccccgcaa
     5101 aagacaactg ggcatttctg gcgttaagtc tctttggtga cgaaagcacg gcacgggatc
     5161 taacgacaca tatcctcgcc tggccacaag aaggcaaatc tgcccgtgcg gtcagtggca
     5221 tgaacatcct taccctgatg aataatgata tggcgctgat gcagctgcat catatatcgc
     5281 aacgggcgaa atcccgcccc ttacgtgata acgcggcgga atttcttcag gtagtcgcag
     5341 aaaatcgcgg gctaagccag gaagagttag cggacagatt agtcccaaca ctgggcctta
     5401 atgatccgca ggcgttgagt tttgattttg gtccccggca gtttaccgtt cgcttcgatg
     5461 aaaacctcaa cccggttatc tttgatcagc aaaacgttcg ccagaaaagt gttccccggt
     5521 tgcgcgccga tgacgatcaa ctgaaagcgc ccgaggcact ggcccgacta aaagggttaa
     5581 aaaaagatgc tactcaggtg agcaaaaacc tgctcccgcg tcttgaagct gccctacgta
     5641 ccacccgacg ctggtcgctg gcagattttc attctctgtt tgttaatcat ccctttaccc
     5701 gtctggttac ccagcgatta atatgggggg ttatccggca aatgaaccgc gtcgtttact
     5761 caacgccttt cgtgtggccg cagaggggga gttctgcaat gcgcaagatg agccaattga
     5821 cctgcctgcg gacgctctga ttggcattgc ccacccgtta gaaatgacag cagaaatgcg
     5881 cagtgaattt gcacagcttt ttgccgatta cgaaattatg ccgccttttc gccagttgtc
     5941 gcgccgcacg gtgctgctca cacctgacga gtcaaccagt aacagcctga ctcgctggga
     6001 aggtaaatcc gctaccgttg ggcaacttat gggaatgcga tacaaaggct gggagtcagg
     6061 ttatgaggac gcatttgtct atgacctggg tgagtaccgg ctggtcctta agttttcacc
     6121 cggttttaac cactacaatg ttgatagcaa agcgctaatg agcttccgtt ctcttcgagt
     6181 gtaccgtgac aataaatccg tcacttttgc cgaacttgat gtgtttgatt tgagtgaggc
     6241 gttaagcgca cctgacgtca ttttccatta aaacaaagcg ggcagtgatg cgactgcccg
     6301 ttatctacac gacttaccag cggggaaagc gatgatcgtg caaaaagagc tggttgctat
     6361 ttacgattat gaggtccctg tacctgaaga tccgttttcc ttcagacttg agatccataa
     6421 atgctctgaa ttatttacag gttccgtcta ccgactggaa cggttccggc tacgtccaac
     6481 atttcatcaa cgtgatcgag aggatgttga cccgctaata aatgatgcgt tgatttatat
     6541 aagagatgag tttattgatg agcaaaaatt acgaggcgaa tcacctgaac ctgtaatagt
     6601 aatttttaat tgtaaactac agaatatatt caaccaacaa atagaattat atactctaga
     6661 taattcaagt cgcagcacat gcaacttgaa gtatgacgag tatagcttta ttaaaggggg
     6721 gaacggtatt cccccttaga tgcgtttcaa tttgcccgtt acttcaacaa atcaatcaac
     6781 cgtaacagag ccactaatag ctccagtaca gcaatgagca cggttaactt ttgtgtcagc
     6841 gtgtccatcc gctctcctta ttaaggagca caacaccagc accttaccct tacactgcct
     6901 gtcgatactg gtttacgttt gttgagtcgc catgttgcac tccggatcag agcactacct
     6961 gcggcaccac ccgtccggta gccaggactt gaaataggtt cgatccggcg caacttccca
     7021 acagtcagga ttataaatct cattatctta attgcaacac tgtccagagg tggttataat
     7081 tcgttcgttg gaacacgatg ttgcacaggc agtggtgtta ggcctgaaaa tagtaaataa
     7141 aagaacctgc ctcaccagca gtttttttta tttactgtga tctgctttcc agatattttt
     7201 cgctcaaaca actaatgcgc caaacattta ttgcgcgtaa aatatcgttt atttcattaa
     7261 tacatttcag ggatgaatat atgtcaccgc agaataatca tctgcagcgt ccgcctgctg
     7321 ctgtgttata cgccgatgaa ctggcaaaat taaaacaaaa tgataacgca ccttgcccgc
     7381 ccggttggca gttaagtttg cctgcggccc gtgcttttat ccttggcgat agcgcgcaaa
     7441 atattagccg taaagtggtg attagtccct ccgctgtcga acgtatgtta gtcactcttg
     7501 ccaccggacg cggtttgatg ttggtggggg aacctggcac cgcaaaatct ctcctgtctg
     7561 aattactggc aacctctatt agcggcgatg ccgggttaac catccagggc ggcgcatcta
     7621 ctaccgaaga tcaaatcaag tacggctgga actacgccct gcttatcaat catggtccat
     7681 caacggaagc gctggtccct gccccgcttt accagggaat gcgcgacggc aaaatcgtcc
     7741 gttttgaaga gataacgcgt acaccgctgg aagtgcaaga ctgtctgtta ggtatgcttt
     7801 ctgacagggt gatgacggtg ccggaactca ctggtgaagc cagtcagctc tatgcgcgag
     7861 aaggctttaa tattattgcc actgccaata cccgcgatcg cggcgtcaat gaaatgagtg
     7921 ctgcgctaaa gcgtcgcttc gattttgaaa ctgtctttcc gattatggat ttcgcccagg
     7981 agctggagct tgtcgccagt gcttcggcgc gtttgctggc ccatagcggt attccgcata
     8041 aagtgcctga cgcggtactg gagttactgg tccgcacctt ccgcgatctg cgtgccaacg
     8101 gcgaaaagaa aacgtcaatg gatacactaa cagcgattat gtccaccgct gaagccgtga
     8161 acgtggccca cgccgtaggc gtccgcgcct ggtttttagc gaatcgcgca ggtgagccag
     8221 ccgacctggt ggactgcatt gccggaacca tcgtcaaaga taacgaagaa gatcgcgctc
     8281 gtctgcgccg ttacttcgaa cagcgtgtcg ccacacataa agaagctcac tggcaggctt
     8341 attatcaagc ccgccaccgc ctgccgtgag gaaagatgca tgagcgagcc gttaattgtc
     8401 ggcatccggc atcatagtcc ggcctgcgcc cggctggtga aatcgttaat cgaaagccag
     8461 cggccacgat acgtgttgat tgaaggccct gctgatttta atgaccgggt agacgaactg
     8521 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
     8581 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
     8641 caagccgatc gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
     8701 agtgaagaag aggacgattc gcctgatacg caagaggaaa gccagacctt gctgctgcgt
     8761 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
     8821 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgactccccc
     8881 ggcgatgctc taaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
     8941 cagcaaaata atggcgacgt gttagttgtc tgcggtggct ggcacgctcc ggcactggca
     9001 aaaatgtggc gcgaatgtcc gcaggtaatt aacacgccag aattgccgtc gctggcagat
     9061 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
     9121 tacctttcag gaatgcctgc cccggtctgg caaaactggt gctggcagtg gggcttacag
     9181 caggcgggag aacaactgct aaaaacggtt ctcacccgtt tgcgccagca caagttgcct
     9241 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcactggc acagttgcgc
     9301 ggtcatacac taccgttacg cactgactgg ctggatgcca tagcaggatc gctgattaaa
     9361 gaagccctga atgcgccgtt gccgtggagc tatcgcggcg ttattcatcc cgataccgat
     9421 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     9481 tctacgccac aaccgcctct gccaaaagat gtcacttgcg aactggaacg taccgcaatc
     9541 tctcttccgg cggagcttac cttaaatcgc tttaacccca atggactagc gcaaagtcag
     9601 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     9661 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     9721 gcggcattga ttgaggccgc ctgctttggt gccacactcc tggaagccgc acgccataaa
     9781 ttagaagccg atatgctgga cgcgggtgga atcggcagca ttaccacatg tcttagccaa
     9841 gcggcgtcag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     9901 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     9961 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
    10021 gcggctatcg atcgcacgct gtggctgtgt gaatccaacg gcagaccgga tgaaaaggag
    10081 tttcacgctc acttgcatag ctggcaagcg ttgtgtcata tcctgcgcga tctacatagc
    10141 ggtgtgcaac tccccggcgt ttcgctttct gcggcggtag ccttgctgga gcgacacagt
    10201 caggcgattc atgtcctggc actggatcgc ggcgcgactc ttggtgcgct gatgcgtctg
    10261 gaacatccca acgctagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
    10321 caatccggcg aggcgctgca cggtttgctg gcattagccc gtcatcaact ggcctgtcag
    10381 ccggcattta tcgccggttt cagcagtcat ttaaatcaac taagtgatgc cgattttacc
    10441 aatgccctgc ccgatttacg cgcggcaatg gcctggctac cgccacgaga acgcgggacg
    10501 ctggcgcatc aggtgcttga gcattatcaa ctggcgcaac ttcccgtttc ggcactgcaa
    10561 atgccgttgc attgtccacc gcaagccatt gcacatcatc aacaactcga acagcaggca
    10621 ctggcatcgc tgcaacactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
    10681 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
    10741 gctggatgac aacgcccggc agatagatca cgcgctggag tggctgtatg ggcgcgatcc
    10801 tgaacggctc cagcgtggtg aacgctctag tggattaggt ggctcaaatc tcaccactcc
    10861 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
    10921 cgatgccgta ctgcgctacg gcattgaaga tgtggtgaca aatctcgacg tgctggaacg
    10981 tatgcagcct tccgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaaccc
    11041 cgaagtactg gctgccgccc gcaggattgt gcgccaggtt gtggaagaaa ttatggcgcg
    11101 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgtcgtc gccgctcatt
    11161 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgccaacc tgcaacactg
    11221 gcacccgcaa cacggcaagt tgtatatcga attcccccgc tttaacagcc gcattaaacg
    11281 ccaaagcgag caatggcaac tggtcttact ggttgatcaa agcggatcga tggtcgattc
    11341 ggtgatccac tctgcggtga tggcggcctg tttatggcag ttacccggca ttcgtaccca
    11401 tctggtggcg tttgacacaa gcgtcgttga tctcacggca gacgttgccg atccggtaga
    11461 gttattaatg aaagtgcaat taggcggcgg aaccaatatc gccagtgcca tggagtatgg
    11521 tcggcaactt attgaacaac ctgcaaaaag cgtcattatc ctcgtgagcg atttttatga
    11581 agggggttca tcatcattgc tgacgcacca agtgaaaaag tgtgtccaga gcggcatcaa
    11641 agtgctggga ctggcagcgc tcgatagcac agcaacacct tgctatgacc gcgatacggc
    11701 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acaccgggcg agctggcatc
    11761 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
    11821 ccgcaggcac tgacggcgtt aagcaatgcc ggttttgtta agcgcagcct taaggaactg
    11881 gaaaatggca acgtcccgga aatcagccat gagaacagcg ctttaatcgc caccttcagt
    11941 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcacctat
    12001 ggggccagcg gcatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
    12061 gctaccgccc agcctacggg aaaagaagag gaatgggatc cggcaatctg gctggaagaa
    12121 ctggctaccc ttcccgatgc cacccgcaaa cgcgcacagg cgctggtcgc taaaagcatc
    12181 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
    12241 gtgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaggcaca
    12301 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
    12361 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
    12421 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
    12481 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
    12541 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
    12601 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
    12661 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggag cgacagtgtt
    12721 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
    12781 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
    12841 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
    12901 agttggccgc gaagtgaaca ggaagactca ccggcagcta cgcgtcggct gtttagtttt
    12961 caggctggcg cactggcggg tgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
    13021 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtaccgct gtcgcctgat
    13081 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
    13141 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
    13201 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
    13261 caggtaatca gcggcgaagg tgaagataat ctgctgacgt tatcactgcc agcctcagcc
    13321 agcgcgcctt atgccattga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
    13381 tgtctggttt ctggctttgt cagttttgtt gatgggcaat tgacactgga accacaggta
    13441 atgatgacaa agactcgtgc ctgggcgctg gacgcagaaa ctgcgcctgt ggtggtatca
    13501 ctaccttctg ccagcgttct gcctgtgccg tctactgctc atcagttgct gatgcgctgc
    13561 caggcgttac ttattcaact gctccataac ggctggcgct atcaggaaca gagcgctatt
    13621 aatcaggcag agttgctggc gaatgacctc accgcggtgg gtttttatcg gctggcacat
    13681 gtgttggcac aatttcgtaa tacagaaagc gaggcacggg tagaagcaat gaataacggt
    13741 gttttgcttt gcgaacaatt attccccatg cttcagcaac aaggatgaaa tagtgctttt
    13801 tactaagagt tctactccag ttccggactg ctcacgccac ggtattaggc atatcctata
    13861 tagcccctgg
//
LOCUS       DAJTYH010000095        13787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTYH010000095 DAJTYH010000000
VERSION     DAJTYH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..233
                     /locus_tag="OO609_003026"
                     /pseudo
     CDS             1..233
                     /locus_tag="OO609_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            293..400
                     /locus_tag="OO609_003027"
     CDS             293..400
                     /locus_tag="OO609_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6634357.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(381..1112)
                     /gene="yehW"
                     /locus_tag="OO609_003028"
     CDS             complement(381..1112)
                     /gene="yehW"
                     /locus_tag="OO609_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6634358.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1117..2043)
                     /gene="yehX"
                     /locus_tag="OO609_003029"
     CDS             complement(1117..2043)
                     /gene="yehX"
                     /locus_tag="OO609_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6634359.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRAWIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(2036..3193)
                     /gene="yehY"
                     /locus_tag="OO609_003030"
     CDS             complement(2036..3193)
                     /gene="yehY"
                     /locus_tag="OO609_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6634360.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3200..4117)
                     /gene="osmF"
                     /locus_tag="OO609_003031"
     CDS             complement(3200..4117)
                     /gene="osmF"
                     /locus_tag="OO609_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6634361.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4369..6666)
                     /gene="bglX"
                     /locus_tag="OO609_003032"
     CDS             complement(4369..6666)
                     /gene="bglX"
                     /locus_tag="OO609_003032"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6634362.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            6862..8577
                     /gene="dld"
                     /locus_tag="OO609_003033"
     CDS             6862..8577
                     /gene="dld"
                     /locus_tag="OO609_003033"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6634363.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8615..9547)
                     /gene="pbpG"
                     /locus_tag="OO609_003034"
     CDS             complement(8615..9547)
                     /gene="pbpG"
                     /locus_tag="OO609_003034"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6634364.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9721..10308)
                     /locus_tag="OO609_003035"
     CDS             complement(9721..10308)
                     /locus_tag="OO609_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6634365.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10478..11056
                     /locus_tag="OO609_003036"
     CDS             10478..11056
                     /locus_tag="OO609_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6634366.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11173..11934)
                     /locus_tag="OO609_003037"
                     /pseudo
     CDS             complement(11173..11934)
                     /locus_tag="OO609_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(11987..13423)
                     /gene="mdtQ"
                     /locus_tag="OO609_003038"
                     /pseudo
     CDS             complement(11987..13423)
                     /gene="mdtQ"
                     /locus_tag="OO609_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            13647..13730
                     /gene="yohP"
                     /locus_tag="OO609_003039"
     CDS             13647..13730
                     /gene="yohP"
                     /locus_tag="OO609_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6634367.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3287 a         3840 c         3471 g         3189 t
ORIGIN      
        1 tggctggaat attcaggata ccacccgtct gtggctggag ggctggattg ccagtcaaca
       61 aggatggcgc attgatgtcc tcgctcgccc actcgctcaa tcaactacgc cctgaactat
      121 tcgaaggccg tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc
      181 aactcaccag ttggcaagaa caaggccatg atattttccc actcggtatt taatgattcg
      241 ttaacaaatg cgctttactg tacaatcctt tcgttaacat aaggagtgca ttatgcgcat
      301 agctaaaatt ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt
      361 catgctcgca ggttatacct ttattttgcg tgctggctaa gcgcctgcac cagcctttca
      421 aacaggcggt ctgcgatgat cgccgccagt gccaccagta acgccccctg gatcacatac
      481 gcggtattaa atccgctaag cccgatgatg atgggcgtac ccagcgtact ggcccctacc
      541 gttgaggcga tcgtcgccgt accaatgttg ataatcaccg aagttcgcac gcccgccaga
      601 atcaccggag ccgccagcgg tagctcgacc ttacgcagtc gctgacctcg actcattccc
      661 atacctttcg caacttctgt cacgctggga tcaatcgctc ccagcccggc aagtgtcacc
      721 tgcaggatgg gcagcacacc gtaaaggatc aaggcgataa tcgctggttg cagaccaaag
      781 ccgatcaccg gaacggcaat cgccagcact gcgacaggcg gaaaagtctg tccaacggcg
      841 gcaatagttt ccaccagtgg gcgaaattcc gcgccccacg ggcgagtaac agcaattccg
      901 gcacctgtgc caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc
      961 agtgccagag ctgcaaaact ttcttgctga taaacgggtc gtggcagttg tgggaacaag
     1021 gcagcaaaca gcggctggct gtaaggcagc cagaaaatca gcgccacaaa aagagcaatg
     1081 agccagaaca gcggatcgcg caacatcttc atacgcttac gcctccacca gcagatcctg
     1141 aaaatgcagc gtgccgcaag gctggccctg cgtgttcacc accggcagca cctcgcatcc
     1201 ccgcgcgaca aacagggaga gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc
     1261 ttctgcccgt tcttcgcgac gcacgtaatc cgccacgcta cgtaacgaaa gcaggtgcac
     1321 ccccagttca ctacgtccaa aaaactggcg gacaaaatca ttcgccggac gagtcagcat
     1381 cgtcagcgga ttcccctgct gcaccacttc accgtgatcc atcaatacca gatgttctgc
     1441 cagccgtagc gcctcatcaa tatcatgagt gaccagcaca atagtgcgcc ccagcaaacg
     1501 gtgaatgcgc gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa
     1561 aggttcatcc atcagtaaga cttgcggatc ggcagccagt gcacgcgcca ctcccacacg
     1621 ttgctgctga ccaccggaaa gctgatgcgg ataacgctca cgcaaatttg actccagccc
     1681 cagtagcgcc attaattcgt cgatacgatc gtcaatccac gcccgcgacc atttttgtaa
     1741 ttgcggcacg gtagcgatgt tttgcgccac gctccagtgg gggaacaggc caatagattg
     1801 aatggcatag cccatccggc ggcgcaactc cagtactggc agcgagcgaa tttcttctcc
     1861 ggcaaagcgg atcactccgc tgtcatgctc caccaggcgg ttaatcattt tcagggtggt
     1921 ggatttgccg gagccagatg tgccaatcag caccgaaaaa ctcccttcct gaaaattgag
     1981 attgagatcg ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc taaattcaat
     2041 cattacgttt caccttcagc agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg
     2101 ccagaacaat taccgggatc acccccagca acactaaatc aatggcgctg cttagcagcc
     2161 cctggaaaac cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc accgccatac
     2221 ctacagtttg caccatcacc acccgcaggc tgcgcagaaa taccggtaac gccagcggta
     2281 actgaacatg caggaaacgc tgcgccccgc tcatccccat cgctctggcg ctctccagca
     2341 catcgcgcgg gatctggttc aaaccgacta ccacgcctcg caccagcggc agcaaggcat
     2401 agagcaccag cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg agcttcccca
     2461 gccacggaaa ggccgtaacc agcgcggcaa gcggcgcaat caacaggcca aagagcgcca
     2521 ccgaaggcac ggtctgaatg acattgagca gagaaaaaat tgccccctgc cgcgcagtgg
     2581 aaaagtagca ccagatgccc aacggcacac caatcactaa cgcaggcagc accgcaccaa
     2641 acagcaacgt cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt
     2701 ctttcattag tgagagatcg ttaagcgtgc cggagtacag caaccacagc ggaataatgg
     2761 caatctgcat atgcaacaac cagcgccaca gcggatgcgt ggagattcgg cggatggcat
     2821 cgctacaggc cagcaatgcc agcgccgcag ccagccagaa acctctgccg aggctggtac
     2881 gcgccagcgc actgccattt tgcgccagtt gggtcgccgc ctttccagct ccccacacca
     2941 acaatacgaa gacgaattgc gccagaatga gtgcaaaaat gctgcctttt ttagcgggga
     3001 caaaacaggc tgtcagccag gcgcaaccaa cgcccaccag catccagatc gtttgcggcc
     3061 acagttgcca gagatgaagc ccttcacccg agactaaacg attaggcgcg taactgataa
     3121 acggcagcgc cgctgcgatt gccgtcagca acaacagcag cgccagaaca ggattaatac
     3181 gtagataagt cacgggaaat tacttcgccc accctttttg tttcaggtag tcggcagcca
     3241 cttttttggc atccagtcct tccactgcaa tgctggcatt cagttgctgc aatgtttttg
     3301 catcgaggct ggcgaagact ggctgtagcc actgtgccat ttgcggatac tccttcaaca
     3361 ccgattcacg caccactggt gcaggcgcgt agataggttg cacgccttgc ggatcgctta
     3421 aggtttgcag ccccagcgcc gcgaccggac cgtcagtgcc gtaagccatt gcggcattaa
     3481 cgccagaggt ttgctgggca gcggctttaa tcgtcaccgc cgtgtcgcca ccagccagtg
     3541 acagcaactg atcctgaccg agcttaaagc cataggcttt ttcaaacgtg ggtaacgcat
     3601 cggcgcgttc gataaactct gccgaggctg ccagtttgaa ggtgccgccc tcttgcagat
     3661 aacgactcag gtcggcaagc gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg
     3721 cgatagtcca ggtgttattt gcaggcgcgg gcgtcagcca gattaacttg ttgtgctccg
     3781 catcgagctt tttgactttc tcgtagccct gcccggcatt tttccacgct gcatcgtttt
     3841 catctttaaa gaagaaagcg ccattgccgg tatattccgg atagatatcc agttcatcgg
     3901 aagtaatcgc cccgcgcccc acaggagtcg ttccgagttg cactttattc accgtcggta
     3961 cgccgtggct ttcgagtacc tgcaagatga tgttgccgag cagcgcgcct tcggtatcga
     4021 tttttgaacc gactttaacg ggggaagccg cctgtagcgg caggctcacg gctgccaaca
     4081 taaccagtga acctgcccag agctttaaga gtggcatgat gctttcctca ttcttttact
     4141 gttgttttca gcgaattaag agaaaagcat agttgataat gacagagtta gcctgagcgg
     4201 aaggattcag ttgcagaatc agataaatac ttaagaggca tattcggtcc ggcgtttcct
     4261 gccggatgcg gcgcgagcgc cttatccggc ctacaaaggg cgaaaattca gcaagttaag
     4321 atatgaacat caggcaaagg gcgcaaacgt cgcgccctta ctaaagcatt acagcaactc
     4381 aaactcgcct ttcttaacgc gtgcggaatc agtgccgata aatacattga acttgccagg
     4441 ctcggcgtca tatttcatct gttgattcca gaacttcagc gcctcaatat cgatcgggaa
     4501 gctgacagtc tgagtttcgc ccggtttcag ggtgattttc tcaaagcctt tcagctgttt
     4561 cacagggcga ctcatggaag ccgtcacatc ctgcaagtac atctgcacta ccgtggcccc
     4621 ctcgcgctta ccggtgttcg tcacctgcac gctggccgtc actttgccgt cacgcttcat
     4681 ggtcggcgca gaaagtttca catcagagac ggtgaaagtg gtatagctca gaccatagcc
     4741 gaacggataa agcgctccgt tagcttcatc aaaataacgc gaagtatatt tgttcggctt
     4801 gtcggcatta tacggacgac cggtgttcag atggctgtag tacaccggga tctgcccgac
     4861 agaacgcggg aaggaaattg gcagcttgcc ggacgggttg taatcgccaa acaatacatc
     4921 ggcaattgca ttaccgcctt cagtccccgc aaaccaggtt tccagaatcg catcagcctg
     4981 ctgatcttct ttcaccagcg ccagcggacg cccgttcatc agcaccagca ccagcggttt
     5041 accggtggct ttcagcgccg caatcaagtc acgttggcct tgcggaatag tgatatcggt
     5101 ccggctggag gcttcgtgcg ccatcccctg tgcttcaccg actacagcca ccaccacatc
     5161 agattgtttc gccgtctgca ccgcttcatc aatcatctct tgcggcgaac gcggatcgac
     5221 tttgaccgct tcttcatact gattcaggaa atcgataatg cctttgtcac tggtaacgtt
     5281 cgcccctttg gcatacagca ctttaccgtt ttcaccgacg gaatttttaa tcccggtcag
     5341 tacggtcacg gattgatcgg caacaccggc tgcggaccag ctgcccatca cgtcacgttt
     5401 actgtccgcc agtggcccaa ccaccgcaat ggtggccgat tttttcagcg gtaacgtttc
     5461 gagacggttt ttcagcaaca ccaggctttc gcgcgccact tcacgcgctt ctttacggtg
     5521 caggcggctt tcggcattgg tatccaccgg gtcagactct ttcggcccca aatggctgta
     5581 tgggtcgtta aacaacccca tatcatattt aacgttcagt acatggcggg cagcatcgtc
     5641 cagctctgcc atggtcactt tgccggattt aatcaaccca ggcagatact tcgagtagta
     5701 ctcgtcgctc atactcatgt tgattccgga tttcagcgcc acgcgcaccg catcttccgg
     5761 gtctgccgcc gtgccatgtt taatcagctc tttgattgca ccgtgatcgg aaacggtgat
     5821 gcctttaaag ccccactggt cgcgcagaac atctttcagc agccaggaat cggaggtggc
     5881 tggcgtgccg ttcagcgagt tcagcgccac catcaccgcg ccgctgcctg cgtccagccc
     5941 cgctttgtac ggcggcatat aatcattaaa caggcgctgc ggactcatat cgacggtgtt
     6001 gtactcttta ccgccttcta ccgcgccgta tgcggcaaag tgtttgacgc tggtcatcac
     6061 cgagtaacga tctgccgggc ttttaccctg catcgcttcc accatggttt tacccattat
     6121 tgaggtgaga tacgtatctt cgccaaaacc ttcggaagca cgtccccagc gcggatcgcg
     6181 cgagacatcg accatcggtg cccaggtcat attcaggcca tcatctgccg cttcataagc
     6241 agagacacgc ccgaccgttt tcaccgcatc gaggttaaaa gacgaggcca gaccgaggct
     6301 aatcgggaac accgtgcgct gaccgtgcag cacgtcgtaa gcaaagaaaa gaggaatttt
     6361 caggcggctt aattccatca cctgatcctg catggcgcgg atatcctgac gggttacggt
     6421 gttgaaaatc gccccaacct gaccgtcttt gatcatctcg cggatcgcct ctttcggatt
     6481 atccgggcct acgctgatta agcgcagctg accaattttc tcatcaaccg tcattttctt
     6541 gagcagatcg gtgacgaacg catcccgcgc ttcaggcgtt aatggatggt taccgaataa
     6601 atcatccgcc agtgctggct gcaggaccag actcaccgcg attcctactg aacatagcca
     6661 tttcatatgg atttttcttc ctttgttgcc cgacgtggca gcgaaaatgg tgcaaaaacc
     6721 gtagtttgcc ataagcatga tggagagaga aaaagaatgc tcagtttatt gtctgaattt
     6781 tcaaaatatt cactcgctga attgtcatac aaggcgctat tctagtttgt gatatttttt
     6841 cgccaccaca aggagtggaa aatgtcttcc atgacaacaa ctgataataa agcctttttg
     6901 aatgaacttg cccgtctggt cggtcattca cacctgctca ccgatcccgc aaaaacggcc
     6961 cgctatcgca agggcttccg ttctggtcag ggcgacgcgc tggctgtcgt tttccctggc
     7021 tcactactgg aattgtggcg ggtgctgaaa gcctgcgtca ccgccgacaa aattattctg
     7081 atgcaggctg ccaatacagg cctgaccgaa ggatcgacgc caaacggtaa cgactatgat
     7141 cgcgatatcg tgatcatcag caccctgcgt ctcgacaagc tgcacgttct cggcaagggc
     7201 gaacaggtgc tggcctatcc gggcaccacg ctctattcgc tggaaaaagc cctcaaaccg
     7261 ctgggacgcg aaccgcattc ggtgattgga tcatcgtgta taggcgcatc ggtcatcggc
     7321 ggtatttgta acaactccgg cggctcgctg gtgcaacgtg gcccggcgta taccgaaatg
     7381 tcgttattcg cgcgtataga tgaagatggc aaactgacgc tggtgaacca tctggggatt
     7441 gatctgggcg aaacgccgga gcagatcctt agcaagctgg atgacgatcg catcaaagat
     7501 gacgatgtgc gtcacgatgg tcgtcacgcc cacgattatg actatgtcca ccgcgttcgt
     7561 gatattgaag ccgacacgcc cgcacgttat aacgccgatc cggatcggtt atttgaatct
     7621 tctggttgcg ccgggaagct ggccgtcttt gcggtacgtc ttgatacctt cgaagcggaa
     7681 aaaaatcagc aggtgtttta tatcggcacc aaccagccgg aagtgctgac cgaaatccgc
     7741 cgtcatattc tggctaactt cgaaaatctg ccggttgccg gggaatatat gcaccgggat
     7801 atctacgata ttgcggaaaa atacggcaaa gacaccttcc tgatgattga taagttaggc
     7861 accgacaaga tgccgttctt ctttaatctc aagggacgca ccgatgcgat gctggagaaa
     7921 gtgaaattct tccgtccgca ttttactgac cgtgcaatgc aaaaattcgg tcacctgttc
     7981 cccagccatt taccgccgcg catgaaaaac tggcgcgata aatacgagca tcatctgctg
     8041 ttaaaaatgg caggcgatgg cgtcggtgaa gccaaatcgt ggctggtgga ttatttcaaa
     8101 caggccgagg gcgatttctt tgtctgtacg ccggaggaag gtagcaaagc gtttttacac
     8161 cgtttcgccg ctgcgggcgc agcaattcgt tatcaggcgg tgcattccga tgaagtcgaa
     8221 gacattctgg cactggatat cgctctgcgg cgtaacgaca ccgagtggta tgagcattta
     8281 ccgccggaga tcgacagcca gctggtgcac aagctctatt acggccattt tatgtgctat
     8341 gtcttccatc aggattacat agtgaaaaaa ggcgtggatg tgcatgcgtt aaaagaacag
     8401 atgctggaac tgctacagca gcgcggcgcg cagtaccctg ccgagcataa cgtcggtcat
     8461 ttgtataaag caccggagac gttgcagaag ttctatcgcg agaatgatcc gaccaatagc
     8521 atgaatccgg ggatcggtaa aaccagtaaa cggaaaaact ggcaggaagt ggagtaaaaa
     8581 ttacggatgg cagagtatcg ccatccgaat tcacttaatc gttctgtgcc gtctgccccg
     8641 ccgccgccat ttgggcggct ttttgttttt tatagctcaa cgctgctgcc ggcacaggca
     8701 tcactttacc ggtttcaatc caggtacgca ggcggctggc atcggcaaaa tgggtatatt
     8761 tgccaaacgc gtccatcact accagcgcca ccggtttatt attgataacc gtacgcatca
     8821 ccagacaatg gcccgccgca ttggtaaagc cggttttggt taactgaata ttccagttat
     8881 cgcgatacac cagatgatta gtattgcgga acggcagcgt atacgtcggg ttagagaagg
     8941 ttgccatatc ttcccgggta gtacttaact gcccgatcaa cggatattgt ttgctggcaa
     9001 tgagcagttt ggttaagtca cgggcagttg aaacgttatg caccgacaat ccggtaggtt
     9061 caacaaagcg cgtgttgttc attccgagcg atttcgcttt cgcattcatt gccttaataa
     9121 aggctttgta accaccggga taatggtgcg caaggcttgc cgccgcgcgg ttttctgaag
     9181 acatcagcgc cagcaacagc atatctttac ggctgatttc gctattcagt cgtacgcgcg
     9241 aatagacccc tttcatctcc ggcgtctggc tgatatccac ttttagtttt tcatccagcg
     9301 gcagtcgtgc atccagcaca accatcgcgg tcattaattt gctgatagac gcaatcggac
     9361 gcaccagatc cgggtggttc gaatagatca ctttgttggt attcagatca acaatcatcg
     9421 cgctaccgga ggcaatttcc ggttgtgaag cggtggtagc ggctgccgtt ttcgcaacgg
     9481 cctgcggtgc aaaaggcaca gccagcatca gggccaagct aaataaagaa actcgaaatt
     9541 tcggcatgat gagcattcag atagtggttc acgcgcacgg gttgcgcacc gccggagtaa
     9601 ggatttactg aggctagcga cgccatcata acgagcaaaa agtgcgatcg tcaaaggaga
     9661 atcgtgagta aatgctgcat tgctgacatt tacgccagca atgcaacgtc aaagaacttt
     9721 ctagaacaaa cgataaccgt agccccatag tataacggtt agggtgagca gcacttccag
     9781 taccagcacg ccaatcgcca gcgtcgaact ggagaagcta aggccttcct ctttgttgat
     9841 attcaggaag ctcggaatac caaggtaaag caggtagccg gtgtaaaaca gcgccaccgt
     9901 gccgaccagc gcacacaacc agaccagtgg ataaagcgcc accagaccgc ttaaaaacag
     9961 cggagttgca acgtagcccg cgaagaccat gcagtgcgca agagacggac gctgcgggta
    10021 attgcgcgcc atccaccaga tgacccgccc catcaccgca accccagcca gcataacgcc
    10081 gtaaaacaag acagccagcg ccagtccggt aaaccaggat aacttcagga tagtgccatc
    10141 gccaaaattc cagccaatct gtgtagtgcc aatgaaggcg caaatcaccg gaatcgccgc
    10201 catcagcaaa acgtggtggg tgtaatggtg agaaatcgtt tcgttttcgc gattaatcac
    10261 ctgcatttca cgatcgggat gggaaaacag tccccagaca tggctcatac cgcccccttg
    10321 ttgtgagttc atgaacctga cagttcaagt ataagccagc ttgtgattat tttttgttca
    10381 tccatgtaat ttgcaccgtc tgatgtatgt cctttttcag cgtgaatgat tcacagggtg
    10441 tatgcttaca ggcaaccaaa gggagacaaa ctggcctatg gatctcaata cactcatctc
    10501 acaatatggt tatgccgcgc tggttatcgg tagcctggcg gaaggtgaaa ccgtgacttt
    10561 gctgggaggc gttgcggcac atcaggggct attaaagttc ccactggtgg tactttctgt
    10621 ggcgcttggt ggcatgattg gcgaccaggt gctctatctg tgcgggcggc ggtttggcgg
    10681 caagctgtta cgccgtttct cgaaacatca ggataaaatt gagcgggcac agaaacttat
    10741 ccaacgccat ccgtatctgt ttgtcattgg tacgcgcttt atgtatggct ttcgggtgat
    10801 tggcccgacg ctgattggtg ccagccagct gccgccgaaa atctttctgc cgctgaatat
    10861 tctcggcgca tttgcctggg cgttgatttt taccactatt ggttacgctg gtggtcaggt
    10921 gattgcgccg tggttgcaca atctcgacca gcatttgaag cactgggtct ggttgattct
    10981 ggtcgtggtt ctggtggtgg gcgtgcgctg gtggctgaaa cggcgcggga agaaaaagcc
    11041 ggataatcag gcgtaaagca gttgccacag ataagcattc actccacatc cggcaccggg
    11101 tgttccatgc ctgatgcgac gctggtgtct tatctggcct aacccattga aatatccacg
    11161 cacctcactg cgctattctg ggttgaactg cggattcgcc aacataaagc cgccatccac
    11221 tatcaacgac tgccctgtcg tgtaatttgc gccctccgaa caaagccaca ccaccaggct
    11281 ggcaatctca tgcgttgcgc caaaacgccg tagaggaatc gaaggctccg cgtcgggctt
    11341 cacgtcgcta tcatccatgc tgttcattgg cgtggctatc gccccaggcg cgactgcgtt
    11401 caccaaaatc ttatgcctga ccaactccag cgccatcgtt ttggttaacc cgccgagcgc
    11461 atgtttagcg gctgtgtagg cgctggcatc cggcagcggc gtatgttcat gtaccgacgt
    11521 aatgttgatg atgcgaccgc cctgccctta tttcaccatc tgacgagccg caatttgcga
    11581 gcataaaaat gcaccatcaa catcaacggt aaaaatcttg cgccactcat caaaagccat
    11641 atcaagaaac ggcgctttgg tcattgcacc cgcattattc accagcacat caatacgccc
    11701 aaatcgctgg atgagtgtct ccagcgcctg cgcaccttct ggcagattgc cgagatccag
    11761 ttgcacgatc tccgcacgta cgccgtgggt aaccacctca cgcgcggtat cttttgcccc
    11821 ttcttcatct gagtgccagg taatgccaat atcaaacccc tgctgcgcca gtaataacgc
    11881 gcactctttg ccgatccccg aatcggaagc ggtaattatc gcgacctatg ccatcgagtt
    11941 ctccacttaa cgctgaataa acgttaagta tagaaggcgc atatcatcag cgtttgtaac
    12001 ccccgcccag cgcaccggtg agtcgaatgg aggcatccag ccactgccct tgcaataaca
    12061 ggccattggc ccgctcacgc agcgccggga ttctggcttc gctgacgcgg gaaccagcaa
    12121 tgatgcccgc gttaaagcgg gcctgcgcaa gacctaccac acgcaaggca tcgcgctcaa
    12181 tttgcgcctg atgctggttt ttctccgcca gtgtctgaac ctgactggct gcccgcgcca
    12241 cgtcattcac tgcttcaacc accgctttgt tgtagctggc gatagacaag ttgctttcgg
    12301 cttttgcgat atcgagattg gcgttaagac gaccactatc gaaaataggt agcgtcaggc
    12361 ctgccgtaac gcccatttgc tgcgcggaat gacggaacag atcgcttaag tgcaacgcat
    12421 cctgttgcag gaaggccatc aggttgatgt caggataaaa tgccgctttt gccgcatcaa
    12481 tagtgcttaa cgatgactca acgtaccagt gcgccgcctg caaatctgcc cgccgggcca
    12541 gtaaggagta ccccagttca tcaggaagct ggcttgccac tttcggcaac gcgaccggtt
    12601 taagcttcaa tgacttagtc tggtgatttg tgagtgcatt taaccgtgcc tcaataattt
    12661 tcattttccc cgcgacatcg ttgagctgct gccgggtttt gctggcatta atatcggttt
    12721 ccacaccgtc aactgaagaa gtaatcccgt tctgatatag ctggcgatcg gtcgcgataa
    12781 tggtgttctg ctctttttct atttgctgca agaccgtgtt taacgccgcc tgggtttgcc
    12841 actcccagta caggcgggct acgctgccag ccagcaattg gcgggtttgc tcgcgttccg
    12901 ccgcccgtgc tttaaccgta cccaggcggg cagtaacctc cgcccgattc tttccccaga
    12961 tatcgagatg ccagcccgcc gttaagccaa aagttccgtt ggtgtaccac ggaccggtcg
    13021 tacctgcggc cggatcgttc agagcaaacg gccccattag cccttctgcc gacatttttt
    13081 gccgctccat atccgccgaa aagtcgatct gcggaccatc ctgagtggca actgccttcg
    13141 cctgggcttc agctagctaa atgcgctgtt cagccacctg catatccggt gcgttctgta
    13201 gtgcattgtt aattaatgaa gtgagctgat tatcgtgata ctccagccac cattggctgt
    13261 ctggccaacc atttttcagc gccgtgggta atgcggtgtc aacttgtgca gcgggcgttt
    13321 gctggcttaa cgcctggcgg gtttcatgca taggcgcaca cccggccagc atcagtaaca
    13381 gcggaaaaca ggcgatggct ggataaaagg aatcacgatt catgggggaa taatcaggta
    13441 agaaacggtg cgcggagatt accgtgtgtt gcgatatatt ttttagtttc gcgtggcaat
    13501 acatcagtag caataaaacg acatacccag aaaaatatag actaagtaaa tgatatcttc
    13561 cgatttatct taatcgttta tggataacgg caaagggctt cgttttttcc tatacttatt
    13621 cagcactcac aaataaagga acgccaatga aaattatact ctgggctgta ttgattattt
    13681 tcctgattgg gctactggtg gtgactggcg tatttaagat gatattttaa aattaattaa
    13741 tgtcatcaga tccgaaaata acgagaatat ttcagtctct catcctg
//
LOCUS       DAJTYH010000096        13755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTYH010000096 DAJTYH010000000
VERSION     DAJTYH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..434)
                     /gene="fabY"
                     /locus_tag="OO609_003040"
                     /gene_synonym="yiiD"
     CDS             complement(<1..434)
                     /gene="fabY"
                     /locus_tag="OO609_003040"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6634368.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPV"
     gene            complement(479..916)
                     /gene="dtd"
                     /locus_tag="OO609_003041"
     CDS             complement(479..916)
                     /gene="dtd"
                     /locus_tag="OO609_003041"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6634369.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(913..1785)
                     /locus_tag="OO609_003042"
     CDS             complement(913..1785)
                     /locus_tag="OO609_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6634370.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(1779..2378)
                     /gene="yihX"
                     /locus_tag="OO609_003043"
     CDS             complement(1779..2378)
                     /gene="yihX"
                     /locus_tag="OO609_003043"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6634371.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(2477..3262)
                     /locus_tag="OO609_003044"
     CDS             complement(2477..3262)
                     /locus_tag="OO609_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6634372.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3296..4192)
                     /gene="yihV"
                     /locus_tag="OO609_003045"
     CDS             complement(3296..4192)
                     /gene="yihV"
                     /locus_tag="OO609_003045"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6634373.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4360..5256
                     /gene="yihU"
                     /locus_tag="OO609_003046"
     CDS             4360..5256
                     /gene="yihU"
                     /locus_tag="OO609_003046"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6634374.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5280..6158
                     /gene="yihT"
                     /locus_tag="OO609_003047"
     CDS             5280..6158
                     /gene="yihT"
                     /locus_tag="OO609_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6634375.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6175..7416
                     /gene="yihS"
                     /locus_tag="OO609_003048"
     CDS             6175..7416
                     /gene="yihS"
                     /locus_tag="OO609_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6634376.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            7554..8456
                     /locus_tag="OO609_003049"
     CDS             7554..8456
                     /locus_tag="OO609_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6634377.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            8655..10691
                     /gene="yihQ"
                     /locus_tag="OO609_003050"
     CDS             8655..10691
                     /gene="yihQ"
                     /locus_tag="OO609_003050"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6634378.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            10737..12111
                     /locus_tag="OO609_003051"
                     /pseudo
     CDS             10737..12111
                     /locus_tag="OO609_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12154..13557
                     /locus_tag="OO609_003052"
     CDS             12154..13557
                     /locus_tag="OO609_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6634379.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            13625..>13755
                     /gene="ompL"
                     /locus_tag="OO609_003053"
     CDS             13625..>13755
                     /gene="ompL"
                     /locus_tag="OO609_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6634380.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     "
BASE COUNT         3322 a         3577 c         3472 g         3384 t
ORIGIN      
        1 acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg ttggcgtggt gatttctccc
       61 tgattaacaa accccagctt ggcgaaaaac tccaccgcgt cttcacgggc gctacaggtc
      121 acgcgcttaa cgccttcctg acgcgccacc gactccaggg tcatcgccat cagcgttcct
      181 aaccctttgt cctgcacgtc cgggtgaacg accataaagc gaatggacgc ttcattgtcg
      241 gcattaatat acaatcggcc taccgccacc agattaccct gctcgtcgac gaccatctgg
      301 tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt ttggttgatg caggggctta
      361 cgcaacattt cccagcgaaa gtgatagtaa cgctctaact cttcttctgt ttgtggaacc
      421 tgaaggtgat acatagcgat actctctctt gtttcccgtg acaaccctgg aagctggctc
      481 atacctgcaa ccagaatgtc acggggccat cattgaccag cgatacctgc atatcggcag
      541 cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca gcgttcgacg aaatagtcat
      601 ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa acttgggcgc atcccccgtt
      661 cggtatctgc ggcgagggta aactgggaaa ccaccagtac actgccgccc gcctgttgca
      721 cgttgagatt catcttgcct tcggcatcgc taaagatgcg gtagccgagc acacgctcgc
      781 acagacggtt tgctttctgt tcgtcgtcat ccttttcgac acccaataac accaaaagtc
      841 ccgcgccaat ttcgcccgtc acttctccct ccacggtgac gctggcacgg gttacgcgtt
      901 gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca gctgcttgtt ttagtttgcg
      961 gtattccccg agagtgacag taatttccgc gccaagcaag acgatacacc atgtccagta
     1021 gacccaaaca aagagaatgg ggatcaccgc cagcacaccg taaatgagct gatatgacgg
     1081 gaacatggtg atataaagcg cgaaaccttt ctttcctgct tcgaacagga gtgcggcgac
     1141 aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg gtaggaacaa tgctgtacag
     1201 caaccagaag gagatccacg acaacagcaa cggaaaaata cgcagcacgt tgtcgatgac
     1261 agtattgaga tcgctcgccc agcgcaggga gagcaaatag gaactgatcg ccagactggc
     1321 ccctgccagc agcggcccca gcgttaaaat catccagtac acggcgaacg agtaaatttt
     1381 tggtcgcgct cgtttactgc gccagatggt attcaacgcg ctatcgatgg agtacatcaa
     1441 caataacgcg gtgacgatca gcccgcacgc cccaacggcg gtcatcttgt tggaattagc
     1501 aacaaattgt tcgatatacc gctgaataac atcgccagta gcaggcagaa agttggcaaa
     1561 aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg ggaaaagcgg cgaaaagcgc
     1621 aaaaacaacg gcaaccagcg gcactaatga gagcaacgac acataggcaa ggttacctgc
     1681 cagggttgtc atgttgtcct catcaatacg ttgccagagt agttttagcc aggcccatag
     1741 tggacgggta cggtgcctgg ctttgtcctg aatggttttt agcataacac cttcgcgaaa
     1801 tagtccggga tggtggtttt atctttcacc agaatactgg taatgcctag ctgattagct
     1861 ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat cgctgggtga aaaaccttcc
     1921 gcctgcaaaa catgctggta aattcgtgct tcaggtttgc gcatccccag atcttgcgac
     1981 agatagatat ggtcagcagc atcacgaatt tccgggtatt cttccggcca gaaggtggta
     2041 tgcaggcggt tggtattgga aagcaccacc acgcgatgcc cctgctcacg cagtttatgc
     2101 atgatggcga tcacttccgg gcgcagcgca acaaacaccg cctgccagcc gtgagagaac
     2161 tgctcgtagc ttagcggtag agccatctca tgacacagcg cctctgcgaa cgcttcgtcg
     2221 ctaatttccc cacgctcatg ctgatgaaac gcctccccca tatgaaaact cttcttaagc
     2281 gatgccagcg gaatacgcgt taaatcgctc caggctccca gcacacggtt aaagtcgata
     2341 tcgacaatca cattacctaa atcaaagata tagagcattt ttgcctcctt tcgcgccaca
     2401 agaaattaac tgtagcggga aaggatgggt ttggctatgt ggcaaggtat gaagccaggt
     2461 taacggtcac aaaaatctac gcgtcttcct ggggaagaat gatttcaatt tggctgtctt
     2521 gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt aaagagcgca gtgacctgtg
     2581 ccacgttacc aatttcaacc gccgccgaag catgatactt agtgtgatcg gcgaccagca
     2641 gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt agcttcgttt acatcaaact
     2701 ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt taccagataa tcagcacgaa
     2761 aatcagccac aaaggacgcc gctgaagggc caatgatccc gctattatga gagcgcaacg
     2821 taccgccggg caccatcact tcaaagcgcg ggttgtggta aagaatatgc gccacacgca
     2881 ggctgttggt gattatccgc aaatgattat ggttaagtaa cgcccgggca acatgctcaa
     2941 cagtcgtacc aatggtgata aatattgttg aaccatcagg aatatagtct gccaccgctt
     3001 cggcaatcgc tttttttttc tcggtttgcg aaacctcacg ctgctcgaac gccgtattaa
     3061 cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt aatcaggcct tgctcgctta
     3121 atttacgaat atcccggcgg accgtctgcg tggagacatc cagcagattt gccagctcat
     3181 caatattcat atacccacgc tcggcgatca gcatgaggag ttggtcgtgc cgcgggttac
     3241 cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac catcaccctg gcattttata
     3301 caaaaagtga caaaaaagat cgggtttgat cacagtcagg gatcccggct cgtccaccgg
     3361 gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg gactgactcc gctaaatccc
     3421 cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac atctcccgca cctgtggtat
     3481 ctaccacatc aactttgaag gccggttgat gctgacgccc accattttcc agccagtcgc
     3541 agcctgcgct accctgggtc acatagacat gaccatttgt gagcgtttgt gcctgtttta
     3601 gcgcactggc catctctttc acgcccgtta agcgcgccag acccggttct gaaaaggccg
     3661 cgtgatcgct taatgccacc agctcactga tatcctgcgg cgtaatgtcc ccgtccagaa
     3721 cggtcatcac acccgcctga cgggccaggg tgaaggcttt tttagcgccg tcgtgccagc
     3781 gtacatctgc cagcacaaca tcccactgag agaaatcaat ttcctccaac cactctgcgt
     3841 caggcagcag atccgggctg gggtagttaa tgattatccg ctcgcctttg gtatccacca
     3901 tgatggcgga ttgcgaagat ttcgcctggt tataccgttt ggtgtaacgg gtgttaacgc
     3961 cccaggattc cagctctgcc agcaggctgt tgcccgcgtc gtcatcacct acgcgaccaa
     4021 taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc tgccgtcgcc gctggcccgc
     4081 caccaacttc cgtataattt ctcgccacgt atttaccgct ctccgtcggt aacccttcca
     4141 cgtaatagat gcgatccatc acggttatac ctacacaagc aacacgaatc atggtcattc
     4201 cttaaacatt ttgggatgcg ttcattttaa tttcaccaat tgtttaaaaa gtgacggctg
     4261 tcaatttttt gacgataaat tacaaattcg atcaaaaaca gacaagcaaa gcatgtcata
     4321 aatgacaaaa agtgacatca ctgtattcag gagagggtta tggcagcaat cgcgtttatc
     4381 ggtttaggac aaatgggttc gccaatggcg agcaatttat tgcagcaagg gcatcaactt
     4441 cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg tagacaaagg tgcgactccc
     4501 gccgccaacc cggcgcaggc cgctaaagat gccgaattta tcattaccat gttgccgaat
     4561 ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg tttgcgaagg cttatctacc
     4621 gatgcgctgg tcattgatat gtccaccatc catccgctgc aaaccgataa attgattgcc
     4681 gatatgcaag ccaaaggctt cagcatgatg gatgttccgg taggccgtac ttctgcaaat
     4741 gccattaccg gtactctgtt actgctggct ggcggcaccg ctgaacaagt tgaacgtgcc
     4801 acaccgatcc tgatggcgat gggcagtgag ttgatcaacg ctggcggtcc gggcatgggg
     4861 atccgcgtta agctcatcaa caactacatg agcatcgcgc tcaatgcgct ttcggcagaa
     4921 gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg ttgccgtcaa agtgatgagc
     4981 ggtaccgccg ccggtaaagg tcacttcacc acttcctggc caaacaaagt cctcagcgga
     5041 gatctttctc ccgccttcat gatcgatctt gcccataagg atcttggcat cgcccttgat
     5101 gtcgccaacc agctgcatgt gccaatgccg ctgggggccg cctcacggga ggtttatagc
     5161 caggcgcgcg cagcgggtcg cggtcgccag gactggtccg ccattctgga acaggtccgt
     5221 gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac tggataaagg aataaatgaa
     5281 tgaataagta caccatcaac gacattacgc gcgcatcggg cggttttgcc atgctggcgg
     5341 tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc tggggcaccc gctccggtag
     5401 ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa ggccctctcg ccttatgcct
     5461 cggcgattct ggtagatcaa caattctgct accgccaggt ggttgagcaa aacgcgattg
     5521 ccaaaagttg cgccatgatt gtcgccgccg atgagttcat tcctggcaac ggtattccgg
     5581 tcgatagcgt ggttattgac cgcaaaatca atccgctaca gatcaaacaa gacggtggca
     5641 aagccttaaa actgctggtg ctgtggcgta gtgatgaaga tgcgcagcaa cgtctggata
     5701 tggtgaaaga gttcaacgaa ctgtgccact cacacggtct ggtaagcatc attgagccag
     5761 tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga acaagcgatc atcgatgccg
     5821 ccaaagagct gggcgacagt ggcgctgacc tctacaaagt tgaaatgccg ctttatggca
     5881 aaggtccgca acaagagctt ctctgtgctt cacaacgtct gaatgaccat atcaatatgc
     5941 catgggtgat cctctcttcc agtgtcgacg aaaaactgtt cccgcgtgcc gtacgcgtgg
     6001 caatgacggc gggcgcatcg ggattcctgg caggccgtgc agtctgggca tcggtcgtcg
     6061 gtttaccaga caacgagctg atgctccgtg acgtttgcgc accgaaatta caacaacttg
     6121 gcgatatcgt cgacgaaatg atggctaaac gccgctaaga tgaggataaa aggaatgaaa
     6181 tggtttaaca ccctaagcca caaccgttgg ctggaacagg aaaccgaccg catctttgat
     6241 tttggtaaaa attccgtagt gccgactggt tttggctggt taggcaataa agggcaaatc
     6301 aaagaagaga tgggcaccca tctgtggatc acagctcgta tgttgcacgt ttattccgtt
     6361 gctgcggcga tgggtcgacc tggcgcttac gcgttggttg atcacggtat taaagccatg
     6421 aacggcgcac tgcgtgataa aaaatatggc ggctggtatg cctgcgtgaa tgacgagggc
     6481 gtggtggatg cctccaaaca gggctatcaa cacttctttg ctctgctggg tgccgccagc
     6541 gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg attacaccat tgagattatc
     6601 gagaaatatt tctggagcga agaagagcag atgtgcctgg agtcctggga cgaagccttc
     6661 agcaaaaccg aagagtaccg cggcggcaat gccaatatgc acgcggtgga agctttcttg
     6721 attgtttatg acgtcactca cgacaaaaaa tggctggatc gcgcgattcg cgtggcttcc
     6781 gtgattatcc acgacgtcgc cagaaataat cattatcgcg ttaacgaaca cttcgatacc
     6841 cagtggaatc cgctgccgga ttacaacaaa gataacccgg cgcatcgctt ccgcgcgttc
     6901 ggaggtacac caggccactg gatcgaatgg ggccgtttaa tgctgcacat ccatgctgcc
     6961 ctggaagccc gttgcgaaca accaccagca tggctgctag aagatgccaa aggtctgttt
     7021 aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg acgggattgt ttataccgtt
     7081 gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt ggcctatcgt cgaagcaatg
     7141 ggtacggtct acgcgctcta caccgtcact ggcgatcgcc agtatgaaac ctggtatcaa
     7201 acatggtggg agtactgcat taagtacctg atggactatg aaaatggttc ctggtggcag
     7261 gagctggatg cggacaataa ggtcatcacc aaagtctggg acggcaaaca ggatatttat
     7321 cacctgctgc attgcctggt gatcccgcgt atcccgttag cgcccggcat ggctccagcg
     7381 gttgcagcgg gtctgctgga tattaatgcg aaataactga tctgaaaata gggtgcgggt
     7441 tttcccctca ccctaaccct ctccccaaag gggcgagggg accgtccggt gcagtttttg
     7501 ggtttgacat cagcctcacg ggaggctgta tgtcgttaat taaggtgcct tgtatgcaaa
     7561 tcaccaatat gcattgcagt ggacagaccg tttcacttgc cgcgggggat tatcacgcga
     7621 ccattgtgac cgtcggtgct ggtctggctg aattaacctt tcagggttgt catttagtca
     7681 tcccgcacaa acccgaagag atgccgctgg cgcatctggg taaagtgctt attccgtggc
     7741 cgaaccggat cgccaacggc tgttaccgtt atcaggggca ggaatatcag ttacccatta
     7801 atgaacatag ttcaaaagcc gctattcacg ggctgcttgc ctggcgagac tggcaaatta
     7861 gtgaattaac caccaccagc gttaccctga cagcttttct gccgcccagt tacggctatc
     7921 cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc acacacgggt ctttccgttg
     7981 agatcgcaag ccagaatatc ggcactgtcg cagcgcctta tggcgtgggt attcatcctt
     8041 atttaacctg caacttaaca tctgttgatg aatatctgtt ccagcttccg gcaaaccagg
     8101 tttatgccgt cgatgaacac gtaaatccga caacgcttca ccacgttgat gagctggatt
     8161 taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat cgaccacacc tttaaaaccg
     8221 ctaatgattt gtgggaaatg accattaccc atccacagca ggcgctttcg gtaagccttt
     8281 gttccgatca accctgggtg caggtttata gcggtgaaaa attacagcgt cagggactgg
     8341 cagttgaacc tatgagttgc ccgcctaacg cctttaattc aggtatcgac ttattattac
     8401 tggaaccagg aaaaccgcac cgcctgtttt ttaatattta cggacagaga aaataacttg
     8461 tattgattag cagtcaaaat cattgaagaa catttcactc gctaaaggca acattatgta
     8521 tcttcaattt attaagttaa aaacagctca gcttctgtaa agtttttata tatcagtttt
     8581 atgagaatca ttttacataa gcctgtaata acaggaaggc aacaatctcg ttctcaactc
     8641 tatggagatt agttatggat acgccacgtc cacagttatt agattttcaa tttcatcaga
     8701 ataacgacag ttttacccta cattttcaac aacgtcttat tttaacccat aacaaagata
     8761 atccttgttt atggattggc tcaggtatag cggatatcga tatgttccgc ggtaatttca
     8821 gcattaaaga taaactacag gagaaaattg cgcttaccga cgccatcgtc agccagtcac
     8881 cggatggttg gttaattcat ttcagccgtg gttctgacat tagcgccacg ctgaatatct
     8941 ctgccgacga tcagggccgt ttattgctgg aactacaaaa cgacaacctt aaccacaacc
     9001 gtatctggct gcgccttgcc gctcaaccag aggaccatat ctacggctgc ggcgaacagt
     9061 tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg gaccagtgaa caaggcgttg
     9121 gtcgcaacaa acaaacctat gtcacctggc aggccgactg caaagaaaat gcgggcggcg
     9181 actattactg gactttcttc ccacagccta cgtttgtcag cacgcagaag tattactgcc
     9241 atgttgataa cagttgctac atgaacttcg actttagcgc cccggaatac catgaactgg
     9301 cgctgtggga agacaaagct acgctacgtt ttgaatgtgc tgacacatac atctccctgc
     9361 tggaaaaatt aactgccctg ctgggacgcc agccagaact gcccgactgg atttatgacg
     9421 gggtaacgct cggcattcag ggcgggacgg aagtgtgcca gaagaaactg gacaccatgc
     9481 gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga ctggtctggg atccgtataa
     9541 cctcttttgg caagcgcgtg atgtggaact ggaagtggaa cagcgaaaac tacccgcaac
     9601 tggattcacg cattaagcag tggtatcagg agggcgtgca gttcctggcc tatatcaacc
     9661 cgtatgttgc cagcgataaa gatctctgcg aagaagcggc acaacacggc tatctagcaa
     9721 aagatgcctc tggcggtgac tatctggtgg agtttggcga gttttacggc ggcgttgtcg
     9781 atctcactaa tccagaagcc tacgcctggt tcaaggaagt gatcaaaaag aacatgattg
     9841 aactcggctg cggcggctgg atggctgact tcggcgagta tctgcccacc gacacgtacc
     9901 tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg gcctgcgctg tgggcgaagt
     9961 gtaactacga agcccttgaa gaaacgggca agctcggcga gatcctcttc tttatgcgcg
    10021 ccggttctac cggtagccag aaatactcca ccatgatgtg ggcgggcgac cagaacgtgg
    10081 actggagtct cgacgatggc ctggcgtccg tagtcccggc ggcgctgtcg ctggcaatga
    10141 ccggacatgg tctgcatcac agcgacattg gcggttacac caccctgttt gagatgaagc
    10201 gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc cttcacgccg atgatgcgca
    10261 cccacgaagg taaccgtcct ggcgacaact ggcagtttga cggcgatgcg gaaactatcg
    10321 cccattttgc ccgtatgact accgttttca ccaccctgaa accttatctg aaagaggccg
    10381 tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc gctgttcctg cattacgaag
    10441 acgatgcaca tacctattcc ctgaaatatc agtacctgtt aggccgcgac attctggtcg
    10501 ctccggtaca cgaagaaggt cgcagcgact ggacgctcta tctgccggag gataactggg
    10561 tacatgcctg gacgggtgaa gcgttccggg gcggggaagt taccgttaat gcgcccatcg
    10621 gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc ggcactgttc gcgtcgttaa
    10681 acagcatcta atcacatccg cccgtgggga aactcatggg caaagggaga acatcgatga
    10741 gtcacatcac aacggaagat ccagcaactt tacgcctgcc ctttaaagag aaactctctt
    10801 acggtattgg cgacctggcc tctaacatcc tgctggatat cggtacgctt tatcttttga
    10861 agttttatac cgacgttctg gggctgccag gcacctatgg cggcattatc tttttgattt
    10921 caaaattctt tactgcgttt accgatatgg gtaccggcat tatgttggat tcccgacgca
    10981 agatcggtcc aaaaggtaag ttccgtcctt ttattctgta tgcgtcattc ccggtcacct
    11041 tactggcgat cgccaacttt gtcggcacac cgtttgatgt caccggtaaa acggtgatgg
    11101 ccactattct gtttatgctt tacggactgt ttttcagcat gatgaactgc tcctacggcg
    11161 caatggtccc ggcaatcacc aaaaacccca acgaacgcgc ctcactggcg gcatggcgtc
    11221 agggcggcgc tacgctgggc ctgctgctgt gcacggtggg attcgtgcca gttatgaatc
    11281 ttatcgaagg taatcagaaa cttggctata tcttcgccgc cacgctgttt tcactgttcg
    11341 gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga gcgttatgtc gaaacccaac
    11401 caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt ccgcgcaatt gccggtaacc
    11461 gcccactgtt cattctgtgt attgccaacc tctgcacctt aggggcgttt aacgtcaagc
    11521 tcgccattca ggtctattac acccagtacg tacttaacga tcccatcctg ttgtcgtata
    11581 tgggattttt cagcatgggc tgtattttca tcggcgtgtt cctgatgcct ggcgcagtca
    11641 gacgttttgg taagaagaag gtctatatcg gcggcctgct gatttgggtg ctgggcgatc
    11701 tgctcaacta tttcttcggc ggcggctcgg tcagctttgt ggcgttctcc tgcctggcgt
    11761 tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca ccgtcgagta cggcgagtgg
    11821 cgcaccggcg tgcgttcgga aggcacggtc tacaccggct tcaccttctt tcgcaaagtg
    11881 tctcaggcgc tggctggttt cttccccggc tggatgctga cgcaaattgg ctatgtgccg
    11941 aacgtcgcac aggctgacca caccattgaa gggttacgcc agttgatctt catctaccca
    12001 agcgcactgg cggtagtcac cattgtggcg atgggttgct tctacagcct gaacgagaag
    12061 atgtatgtcc gcattgtgga agagatagaa gcccgtaaac gcacggcgta ataataataa
    12121 acgacgccct gcggggcgtt ataaggagtg attatgtctg accataatcc actgacatta
    12181 aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg acgtcggttc gaatttaatg
    12241 ctctgcatcg gtactctgta tctcctcaaa ctttataccg atgaattagg gatgcctgct
    12301 tactatggcg ggattatctt tctggtcgcg aagtttttta ccgcgtttac cgatatgctc
    12361 accggatttt tactcgactc gcggaaaaat attgggccta aaggcaaatt tcgtcccttt
    12421 attttatatg cagcagttcc ggcggcgtta attgcaacgc tccagtttat cgccaccacc
    12481 ttttgtttgc cggttaaaac gacgattgcc accacgctgt ttatgatgtt tggactttca
    12541 tacagtctga tgaactgctc gtatggtgcg atgatcccgg caattaccaa aaacccgaat
    12601 gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa ccatagggct attgatttgt
    12661 accgtggcgt ttattccgtt gcagtcgctt ttttctgact caaccgtcgg ttatgcctgt
    12721 gcggcactta tgttctccat tggcggcttt atttttatga tgctgtgcta cagaggcgtc
    12781 aaagagcatt atgtggacac agcgccaacc ggacataaag ccagtattct caaatctttt
    12841 tgcgcgatat ttcgtaatcc accattgctg gttttatgca ttgctaacct gtgtactctg
    12901 gcggcattta atatcaaact ggcgattcag gtctattaca cccagtatgt gctgaatgat
    12961 attaatttat tgtcgtggat gggattcttc agtatgggat gcatcctcgt cggcgtatta
    13021 ctggtgccag taactgtaaa atgttttggt aaaaaacagg tttatttagc tggcatggtg
    13081 ctatgggcgg tgggtgatat actgaattat ttctggggaa gtaactcttt cactttcgtc
    13141 atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa acagcctgaa ctgggcactg
    13201 gtaccagata ccgtagatta cggtgaatgg aaaaccggta ttcgtgccga aggttctgtt
    13261 tataccggtt ataccttctt tcgtaaaatt tctgccgcac ttgctggctt cttgccaggc
    13321 attatgctga cgcaaattgg ttatgttccc aacatcgcgc aaagcgatgc gacattacag
    13381 ggtttgcgtc agctcatttt tatctggcct tgtgcactgg caattattgc tgcattgacg
    13441 atgggattct tttacacact caatgaaaaa cggtttgcat tgattattga ggaaatcaac
    13501 caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg cttccgtaac gttataaatg
    13561 tcaatagccg ctatttccat cctggtgggt ggcggcctcc ctacgtttaa aaaatggact
    13621 tattatgaaa aagattaatg caataatttt attatcttct ctgacctctg cctcggtatt
    13681 tgctggcgca tacgtcgaaa acagggaggc atataatctt gcctccgatc agggcgaggt
    13741 tatgctacgt gtggg
//
LOCUS       DAJTYH010000097        13694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTYH010000097 DAJTYH010000000
VERSION     DAJTYH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(5..508)
                     /locus_tag="OO609_003054"
     CDS             complement(5..508)
                     /locus_tag="OO609_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6634381.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(553..1516)
                     /locus_tag="OO609_003055"
                     /pseudo
     CDS             complement(553..1516)
                     /locus_tag="OO609_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(1830..2966)
                     /gene="hemW"
                     /locus_tag="OO609_003056"
     CDS             complement(1830..2966)
                     /gene="hemW"
                     /locus_tag="OO609_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6634382.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(2959..3552)
                     /locus_tag="OO609_003057"
     CDS             complement(2959..3552)
                     /locus_tag="OO609_003057"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6634383.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(3560..3850)
                     /locus_tag="OO609_003058"
     CDS             complement(3560..3850)
                     /locus_tag="OO609_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6634384.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(3847..4413)
                     /gene="yggT"
                     /locus_tag="OO609_003059"
     CDS             complement(3847..4413)
                     /gene="yggT"
                     /locus_tag="OO609_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6634385.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(4431..5135)
                     /gene="yggS"
                     /locus_tag="OO609_003060"
     CDS             complement(4431..5135)
                     /gene="yggS"
                     /locus_tag="OO609_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6634386.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            5153..6133
                     /locus_tag="OO609_003061"
     CDS             5153..6133
                     /locus_tag="OO609_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6634387.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            complement(6309..6725)
                     /gene="ruvX"
                     /locus_tag="OO609_003062"
     CDS             complement(6309..6725)
                     /gene="ruvX"
                     /locus_tag="OO609_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6634388.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6725..7288)
                     /locus_tag="OO609_003063"
     CDS             complement(6725..7288)
                     /locus_tag="OO609_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6634389.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(7397..8347)
                     /gene="gshB"
                     /locus_tag="OO609_003064"
     CDS             complement(7397..8347)
                     /gene="gshB"
                     /locus_tag="OO609_003064"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6634390.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(8360..9091)
                     /gene="rsmE"
                     /locus_tag="OO609_003065"
     CDS             complement(8360..9091)
                     /gene="rsmE"
                     /locus_tag="OO609_003065"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6634391.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(9171..9878)
                     /gene="endA"
                     /locus_tag="OO609_003066"
     CDS             complement(9171..9878)
                     /gene="endA"
                     /locus_tag="OO609_003066"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6634392.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(9973..10470)
                     /locus_tag="OO609_003067"
     CDS             complement(9973..10470)
                     /locus_tag="OO609_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6634393.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            complement(10547..11941)
                     /gene="galP"
                     /locus_tag="OO609_003068"
     CDS             complement(10547..11941)
                     /gene="galP"
                     /locus_tag="OO609_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6634394.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(12378..13532)
                     /gene="metK"
                     /locus_tag="OO609_003069"
     CDS             complement(12378..13532)
                     /gene="metK"
                     /locus_tag="OO609_003069"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6634395.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         3362 a         3462 c         3498 g         3372 t
ORIGIN      
        1 tccattaact ggcctcctgc gcaggttgtt tcagttgttg aatgctttga ttaataaaac
       61 acaaggcata aaacatcatt accactccac ttaatggcag aatgatatat acgtatccca
      121 tcggtatttg catagcggca gatacctgtg atgttgccag tgttttatca attaatttca
      181 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat gagaacgtta atgactatac
      241 taagaagtaa ttgtttgcgc ttattcaggg cgagagcaaa taaatcaata gagagatgtc
      301 gctgcgcgcc gacggtatat gcagcaccta ataatcctac ccagatcatc aaaaagcgtg
      361 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
      421 ctacgcatac aaccagagca agcattacgg cgacagaaaa cgtggcaatg acgcggtcta
      481 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagaggc
      541 tggcccggaa tattattgag cagcgttgtc gaatttctct aacaaagcag cttgttttgg
      601 atcttttttg aagtcgtcaa acagaggttg cacggcttta cggaatggcg ctttatccac
      661 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
      721 ccacaatttt tgttgataag cttcggattt cttcgccgct gtttcgagga tttgttgctg
      781 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
      841 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca tccaggaagg
      901 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
      961 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
     1021 ctgaacacga attttcagtc ctttaagatc ttctggcttt gtaatcggtt ttttcgcata
     1081 gaaactgcga gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
     1141 gtccataatt tctttaccaa cttcaccaaa aacaacttaa taagaacggc aaattataga
     1201 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
     1261 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1321 tacggatcac catattacct tttgacagct gttttacctc tttcgccagc tcttcaaaac
     1381 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgcc actttttctg
     1441 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1501 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1561 tgtgcttatc agtgatttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1621 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
     1681 atttataatc aatgaatgat tagtaaatat tcactaaata tatataataa atgtcgtcga
     1741 tatttattca gtaagaaata tatttctgcc gaggttaatt aacgggggga taaaagaagt
     1801 gattaatgcg cccgcaggcg cattaatgat tactcagcga ggaataattc aagaagtgag
     1861 tttaagaata atttaccatg ttccgttatc tgccagtaat ccgcacattc ggtgagataa
     1921 ccctgggcaa tcgcctcgtc taactgtggg cgaatcacat cttcgcaaag cccggtatac
     1981 gcaataaact ccacgcgcgg cgcggcctcc agcagacgga agcgattcat aaagaactca
     2041 aacggcttat ctgcggcttc gacatcacgc tggctttcca gataccttcc ttgcataaaa
     2101 ccacgaggat gacgcgtttt agtggtacgc agaatgcgcc catccgggaa ggtcactttg
     2161 ccgtgcgcgc cgcagccaat accgatgtag tcaccaaagc gccagtagtt gagattgtgc
     2221 tggcactgat aaccgggttt ggcgtaagcg gaagtttcat attgctgata acccgctgcg
     2281 gttaataact gatgcccctg ttcgaaaata tcccacagcg cgtcatcgtc cggcagcacc
     2341 ggcggtcgcg agccaaacag cgtattaggt tcgatagtca gttgatacca ggaaagatgc
     2401 ggcggattca gttcaatggc ctggcgtaga tcgccaagcg cctcttccag tgattgatcc
     2461 ggtagtccat gcatcaaatc aaggttaaag ctacgtaacc ctaaaccgct cgccagcttt
     2521 gccgctcgtt tcgcttcttg cgggccatga atacgcccaa gtcgtttcag cttttcttcg
     2581 ctaaaactct gcacaccaat agagatgcgg tttacaccag cacgctgata atcgacaaag
     2641 cgatccgctt ctaccgtgcc tgggttcgct tccatagtaa tttctgcatc cgctgccagc
     2701 ggcaaacgcg cacgcacgcc gtctagcagc gtttgcatcg ccgggccgga aagcaggctc
     2761 ggcgtaccac cgccaataaa gattgtcttt acttcacggc cctgagcgta agccacatca
     2821 ttgtccagat cgcaaagcag atgctgaaca taatcgtcgt gcggcacttc tcctttcaac
     2881 gcgtgagagt tgaaatcgca gtacgggcat ttctgcacgc accacgggat gtgaatgtag
     2941 agactcagcg gcggtaattt aaccattacg taaagcgtcc agcagcagtt tcaacgcctg
     3001 accacggtgg gaaatggcgc tcttttcttc gcgggtcagt tcggcggcgg ttttcccttc
     3061 ggaaggtacg aagaagattg gatcataacc aaagccacca gtgcccgctg gttcgcgagt
     3121 aatcacgccc ggccagctgc cgtggcatac cagcggagtc ggatcttccg cgtgacgcag
     3181 atataccagc acgcagtgga aacgcgcctg acgttggtcg tccggtacgt ctttcagtgt
     3241 ttccaacagt ttttgcagat tcttttgatc ggtcgcgtct tcaccggaat aacgcgcgga
     3301 gtaaataccc ggcgcgccac ccagcgcatc taccgccaga ccagagtcgt cggcaattgc
     3361 cggtaaaccg gtcacttttg ccgcatggcg cgctttcaga atcgcgtttt cgataaaggt
     3421 taggccggtt tcttcagcgg aatcaacgcc gaggtctgtt tgggccacga tatcaagacc
     3481 aaagtcgcta agcagcgacg ccagctcacg cactttaccg gcattgccgg ttgcgaggac
     3541 aactttttgc ataggatacc taattaatta acgccgcgat ttctggcggg atttgttgcg
     3601 gattaatgat tttaatttgt ttgtggcggc caagttcgcc tttttcaatc accacctggc
     3661 ttttggcaac ccggaattgc ttaccgagaa acttcaccag atgactgttg gcctggccgt
     3721 caaccggcgg cgcggtaatg gcgactttaa cttcgtcgcc atgtaaaccg acaatagaat
     3781 cacggctggc tttcggctga atatagagcc gtaaaaccag accgtcatca ttaactgtta
     3841 cggcactcat aacgccatcc acagccccgg cagcagcatg tttccggttg cctgtaatac
     3901 ttctgcgaca cccatattga tggcatacag cagcagaacg aggatcatcg gcgagaaatc
     3961 aatcccaccc attgccggta gcaagcggcg aatcgggcgc agcagcggat cggccagctg
     4021 aatcagcacg tattcaatcg ggctacgacc ctggcttacc cagctcataa tcgccatcac
     4081 cagcaggacc cagaaaatca gcaagccgat ggttttcagc agaatcagta aaccggcaat
     4141 ccagatgatt ggcaggaagg tcaccacttc aaacagcaca atggctttga taaaactgag
     4201 aatataggca accagcagcg aggcgctgtc aatgggaccc attgccggaa taacgcggcg
     4261 cagtggcccg ataattggct gcgctacctt cactacaaac tgtgagaagg gggtgtaaaa
     4321 atcacaatga gcacactgca tccagatgcg taataacagc accattgtat acagctcaat
     4381 gaccgttgaa agcaggaaag tcaacgtatt catggcgttc ctttaattcc ttatttttta
     4441 gagtaatcac gcgcaccaaa aattgcagtg ccgatacgaa ccatcgtgct acctgccgca
     4501 atggcggctt ccatatcgtc cgacattccc agagagagcg tgtcgatatg cgggtagcgc
     4561 gttttcagtc cggcaaatgc tacagccatt tggcgtgcaa cttcaaactg ccttacatat
     4621 tctgactcaa gcgcagggat tgccatcagc ccgcgcagac gtaaacgcgg tagttcagcg
     4681 accgccgctg ccagctcgtc cagttctgcc agttgaatcc cggacttact gttttcatca
     4741 ctaatattaa tttgaatcag tacgttaaga gggggaagtt ctgccgggcg ctgctcgttg
     4801 aggcgggtag caatgcgcaa acggtcgatg gtatgacacc agtcgaaatg ctctgccact
     4861 aggcggcttt tattagactg caacgggcca ataaaatgcc attctaatcc tgttacgccc
     4921 agttcctgaa agtggcgaat tttatctacc ccttcctgaa cgtagttttc accaaattga
     4981 cgctgcccgg catcaatggc ttctgcgatg gcgctcgcag gttttgtttt actgactgca
     5041 agcagcgtaa tttcttctgg agaacggccg caacgcgttg cagccgctga gattttgtcc
     5101 cggacctgtg ccaggttatg cgcaatatcg ttcattttcc gaggatctta gtatgaatat
     5161 ggaagaaatt gtggccctta gtgtaaagca taacgtctcg gatctacacc tgtgcagcgc
     5221 ctggcccgca cgatggcgca ttcgtggcag aatggaagct gcgccgtttg atgcgccgga
     5281 cgtcgaagag ctactgcggg agtggctgga tgacgatcag cgggcaatat tgctggaaaa
     5341 tggccagctg gattttgccg tgtcgctggc ggaaaaccag cggttgcgtg gcagtgcgtt
     5401 cgcgcaacgg caaggtattt ctctggcatt acggttgtta ccttcgcact gtccacagct
     5461 cgaacagctt ggtgcaccac cggtattgcc ggaattactc aagagcgaga atggcctgat
     5521 tctggtgacg ggggctacgg ggagtggcaa atctaccacg ctggcggcga tggttggcta
     5581 tcttaatcaa catgccgatg cgcatattct gacgctggaa gatcctgtgg aatatctcta
     5641 tgccagccag cgatgtttga tccagcagcg ggaaattggt ttgcactgta tgacgttcgc
     5701 atcggggttg cgggctgcat tgcgggaaga tcctgatgtg attttgctcg gagagctacg
     5761 tgacagtgag acaatccgtc tggcgctgac ggcggcagaa accgggcatc tggtgctggc
     5821 aaccttacat acacgtggtg ccgcgcaggc agttgagcga ctggtggatt catttccggc
     5881 gcaggaaaaa gatcccgtac gtaatcaact tgcgggtagt ttacgggcag tgctgtcaca
     5941 aaagctggaa gtggataaac aggaaggacg cgtggcgctg tttgaattac tgattaacac
     6001 tcccgcggtg gggaatttga ttcgcgaagg gaaaacccac cagttaccgc atgttattca
     6061 aaccgggcag aaggtgggga tgttaacgtt tcagcagagt tatcagcagc gggtggggga
     6121 agggcgtttg tgacgttgcc atgcccgatg cgcaagttta tagggcctat aaaggcaata
     6181 aatgtctgat gcgacgctaa cgcgtcttat cagacctaca gccgctgcca gatgtgtgct
     6241 ggataaggcg ttcacgccgc atccggcaag atggcggtaa cgactatccg ccaggctttt
     6301 agatcgcctt aatatccctg ctcgaaatag ctttcgagaa taataaccgc agaggcagag
     6361 tcaactttgc ctttgttgag cgcccgatag ccgccctgtt caaaaagacc ggaacgggct
     6421 tccacagtgc taagacgctc gtcatggagc tttacttcaa caccgaaacg accatgaata
     6481 cggttggcaa atttgcgcgc tctggcggtt aacggctgtt cggtgccgtc catattcagc
     6541 ggcaaaccga cgatgatttc gtcaggctgc cactctttca gtaaacgttc gatgaggttc
     6601 cagtccggcg taccgtcctg cgctttaatt gcaggcaaag ggcgagcggt gccggtaatg
     6661 cgttggccga ccgctacgcc aatgcttttg gtgccgaagt cgaaggcgag taaggttcca
     6721 ctcatcaggc gtgtcctgcc acaccaggca tggtgagaat atccacacca atcagttttg
     6781 ccgcctcgcg ccagcggtcg gcaatcggcg ttttgaacag aatattcaga tccgccgggg
     6841 ccgttagcca cgcgttatcg agaatttctt gttccagttg acctttttcc caggaggcat
     6901 agcccagagc caccaatacg tcagacggtt gtttatcggt gccgagcgtt tccagcacat
     6961 cgcgggaagt ggtcatcacc gtgttgtctg aaatgcgaat gctggaagca aaattggagg
     7021 gcggagtatg caaaataaac ccgcgatctt cagccagtgg gccgccgagc ataaccggtt
     7081 tatccagacg gattgattca tcacgcggct ccggcgtgat cttcagcttt tccagaatcc
     7141 cttcaatttt gagattttcc agcggcttgt tgacgatgat ccccattgca ccattggtat
     7201 tatgttcgca aatgtagacc acggaacgac ggaaaatcgg atcctggaga gcaggcatgg
     7261 caataagaaa gtgatgctgt aaattcattg tcagaggttc tgtttcctgg ttcaaaaaag
     7321 caacagcgcc cagtatgcgt ggaaagaagg gcgctgtcac tcagagtctc aacgagatcc
     7381 ttctcgctaa ggtgggttac tgctgctgta aacgtgcttc gatggcatcc attaacattc
     7441 cggtgatcga caccggaaac tctgcttcaa tctcacgaat acaggttggg ctggtgacgt
     7501 taatttcagt cagacggtcg ccgatgatat ccagaccaac aaaaatcagc cctttttctt
     7561 tcagcgtcgg cccgatctga cgggcgattt tccagtcact ttccgtcagc ggacgaggtt
     7621 caccgcgacc accggcagcc agattgccac gggtttcgcc cccctgcgga atacgcgcca
     7681 ggcagtacgg taccggctcg ccatccacca ccagcacgcg tttgtcgcca tctttaatgg
     7741 ctggcaggta attttgcgcc atgcagtagc aagtgccatg ctcagtcagg gtttcggcaa
     7801 tcacgccgag gtttggatcg ccttctttca cgcggaaaat cgacgcgccg cccataccgt
     7861 ccagcggctt aagaatgatg tcgctgtgtt tctcccagaa cgcttttagc tgcgctttat
     7921 tgcgcgtaac cagcgtttct ggcgttaagt cagagaacca ggcggtaaac agtttctcgt
     7981 tacagtcgcg caggctctgc ggcttgttaa cgatcagcgt ccctttctct tcggcacgtt
     8041 ccagaatata ggtcgcgtag ataaactcgg tatcaaacgg cgggtcttta cgcatcagga
     8101 tcacatcgag atcggccagc ggcagatcct gttcaccgac gaacgaaaac cactcttcgt
     8161 agttctgctt cacgttcagc gtgcgggtat gggcgcgggc ttcgccattg atcagataca
     8221 gatcgcccat ctccatatag tgaagttcgt aaccacgacg ctgtgcttcc agcaacatag
     8281 cgaaactgga atctttcttg atgttgatgt ttgcgatggg gtccatcacg atgccgagct
     8341 tgatcattat tcttctccgt tagcccaaat cgccaaatcg tacttgtagc gcggtaatgg
     8401 cggtgagcgc agttgtctct gtacgcaaaa cgcgaggtcc caacaggata tcagtaaatt
     8461 gatagcgggc agtcatggca atttcatctg ccgataaacc gccttccggg ccaatcagca
     8521 ggcggacgcg ttcaaccggt aaaggcaacg tattgatgct gttactggcg cgcgggtgaa
     8581 gattcagttt cagtccttca tcctgctctg cacaccaggc ttccagatcc atcgctggac
     8641 ggatttccgg cacccggtta cgaccacact gctcacaggc agcaattgca atcttctgcc
     8701 actgctgaag cttcttgttc agacgttcac tatccagttt aacgccgcag cgctcagaaa
     8761 aaagtggcgt aatgaggctt acaccgagtt cgatcgattt ctggatagta aattccattt
     8821 tttcaccacg cgacatcacc tgaccgaggt gaatatgcag cggagattcg cgatcgtcga
     8881 tctggccttc cagcaccttc acttccacgc tttttttgct ggcgctggta atttcggcgt
     8941 caaagacctg gttgctaccg tcaaacaatt gcaacgcctg ccccggcccc atgcgcagta
     9001 cgcgcccgat atggttggcg gcatcttcgc aaagcgcgat gtgagaatgg ctggtcagtg
     9061 gttcaggatg ataaatgcgg gggatacgca tagttaaaaa tccgcgtcgt ctccccacgc
     9121 ggttgtacgc gtggggtagg ggttaacaaa aagaatcccg ctagtgtagg ttagctcttt
     9181 cgcgcctggc aagcgcgttg cacatacggg ttatgattgc cctgcacctt cgcgatgcgt
     9241 tcatcgcgct cgcactccca gtcggtaacc ggatacatct tgttccatgc gttgaacagc
     9301 tgcgtttgct ggcgagagag tgtcaggttg tattggtcgc gcatatagaa gtaggtgcgc
     9361 gcaatggcac cgcgtgcacg cgctggtggt tcggcagctt tttctttgaa atcgaccttc
     9421 atggcgcatt gaccgtactg gccttcgccg ccattccact ggctgtacat aaaattgccg
     9481 cgatcgccat tcacctcgcc gactgacggc tgcaggttat gcatatcgct ttccatcttg
     9541 cgatagaccg gatctttagc gcagttttta cgtccaccgt cctgccagca ctggcgctgg
     9601 tgaccgaatt gccaggcggg aacgacgtgt tcccactcta cgcggctggc gcggttttca
     9661 tttttgcgca cctgatagcc gcacgattgc agatcaacaa cgcctttttt gccctgccag
     9721 ttaattttac atccgcaata aaacgtaccg ggcgcatcag cgtggacttt taccgccgcg
     9781 gctttcgcct gagaaaaact attgatacct tcggccaacg tcgggccgga aaatgctgcg
     9841 ctcagtacca ccgcagcaat agacaaataa cggtacatca cttactccgt gttaaaacga
     9901 gccagcaacg tagcgaaagt tgttttggta tgcaatcagc cagatcagga aagttcctga
     9961 tagttcaggt ggttatttcg caaccagctg ttcaccgcag tgaacacagc gatagatggc
    10021 ctcgccacga actacgcgat tatggcggcg tacggtaagc tgatgttcct ggcacttaca
    10081 gcggtagggg aaggtgttgc gacgcacgga ttgcagttcg aactgatgcg tacgacgggc
    10141 gggaacgccc agcacgcttt ccatcatcca cttccactct ttgccatgtg gcgctacgcg
    10201 gccgaaatgt ttccatacca gcaaatgtgc cagttcgtgc ggtaccactt cttcaataaa
    10261 agcttcactg ttttccaaca gcaaaacggg attgaggcgg atttcatagc tttccagcca
    10321 ggctgttccg gcggaggttc cgcgctgggt gtaagagagt tttggctccg ggtagttacg
    10381 ccctagcttc aggttggcct gggcgagttt ttcccgcagg cggcgcataa cggcctgttg
    10441 gatggcgata gggagacggg atgttttcat agcggcagag gatagagcga agaaaagatg
    10501 actgcaagag gcggcttcct ccgcgacggg aggaagcctg aggagattaa tcgtgagcgc
    10561 cgatttcgcg cagtttacga cctttcatca gattacgttc aatatgttcc agcgaaacgt
    10621 gtttggtttc cggtaccagc cacaatgtca gcaggataaa cagtacgttc agagccgcat
    10681 acacccagaa ggtgttggcg ttacccagcg tgttgagcat ggtcaggaac gttgcgccaa
    10741 cgatcatgtt ggcaatccag ttggtggcgg tggagcaggt gatgccaaaa tcgcggcctt
    10801 tcagcggctg aatttcggag cacagtaccc aaatcagcgg accggcactc atggcaaaac
    10861 cgacaataaa catcagcagc atggcgatgg cgaaatactg cgccgacgga gagtggatac
    10921 cgatatgcat cattgtaccg agtacgccca tgccagcagc catcaccagg aagcccagcg
    10981 ttagcgttgg tttacgtccc cagcggtcaa caaggccgat tgcgataaag gtggcaagta
    11041 cgttggtcag gccgacaatc actgtccccc acatttgctc ggtggtgttg gtataacccg
    11101 ccagttcgaa gattttcggc gcgtaataca tgatgacgtt catcccggtg aattgctgca
    11161 ttacctgtaa cagtacgcca aggaacaccg cgcggcggaa gttgctattc tctttaaaca
    11221 gcgcccagcc actctgttta acctgcaaac tttcacggat ttcatccagt tcgcgtttcg
    11281 cttccgcgct ggtgtcacgc aggcgtagca gcacgcgttc ggcatcaaca aaacggcgtt
    11341 tggcggcaaa ccaacgtggg ctgtctggca ggaagaagac accaatcagc agcaaaattg
    11401 ccgggatgat aatcacaccc agcatccagc gccatgcacc ggtgtagctg aaggcggtat
    11461 cagaaagata agcaccgagg atcccgatag tgatcatcaa ctgatacatc gagatcatac
    11521 tgccgcgaat tttttccggc gcaatttctg acaggtacag tggtgcggta taagaggcca
    11581 cacccaccgc cagccccagc agaacgcgcg aaagaatcag tacttcaacg tttggcgcag
    11641 ccgcagagaa cagtgaaccg gcaacaaaca ggattgcgcc gatcatcagg ctctttttgc
    11701 gcccgagttt aaaggagagc cagccgatgc ccaccgcacc gactgccgca ccgaacatca
    11761 tggagcttac gacccattct tgcgtgtgcg aagttatctg gaattcatct gcaataaacg
    11821 gcagtgcgcc agcaattaca ccgatatcca ggccaaagag taatcccgcc agagcggcaa
    11881 ggaagcagac gaaaaacgtc attgccttgt ttgaccgccc ctgtttttta gcgtcaggca
    11941 tgatgccctc caatatagtt attttttatt gtgaattaag ataggtgagt acgacgtaaa
    12001 aagatgtgaa gcaaatcaca tcagtgtaat cgcttacact aacatttatc ttagttatga
    12061 tttaagggca tgaaaataat tgagttatat catcatagat taaacgctgt catctgcaat
    12121 taagacttta ctgaaaagaa atgtaacaac tgtgaaagct gaataataat atgaaaatat
    12181 tacaaatatt ttgctggtgt ggttggtgta atcggtttca gttgtgatgg taatttgccg
    12241 tgaaacgtga tcaggaagaa tgatgttatt gtgcgggcag tttttttgcc ggatgcggcg
    12301 tgaacgccta tccaccgcgg tgaggtaaaa gaaaaaggcc agcgcgaggc tggcctttga
    12361 acgcaggtga agaaagatta cttcagaccg gcagcatcgc gcagcagctg cgctttgtcg
    12421 gttttttccc acgggaaatg ttcacgacca aagtgaccgt atgctgcggt ttctttgtag
    12481 atcgggtgca gcagatccag catctgaatc agtccgtatg ggcgcaggtc gaagaactca
    12541 cgtaccagca gagtcagttg ttcagaaggc actttctcag taccgaaagt ttctaccatg
    12601 atggaggtcg gttcagccac gccgattgcg taggaaacct gaatttcaca acgatcggcc
    12661 aggccagcag caacgatgtt tttcgcgaca taacgtgctg cgtaggctgc ggaacggtcc
    12721 acttttgatg gatctttacc agagaatgca ccgccaccgt gacgcgccat gccgccgtag
    12781 gtatcaacga taattttacg gccagtcaga ccgcagtcac ccattgggcc accgataacg
    12841 aaacgaccgg tcgggttgat gaagaatttg gtggcagaag tcagccattc agcgggcaga
    12901 attggcttga tgatctcttc cattaccgct tcttgcagcg atttctggtc gatctcttca
    12961 gagtgctgag tggaaagcac gacagcatca ataccaacga ttttgccgtc gtcatactgg
    13021 aaagtcacct ggcttttcgc gtccgggcgc agccacggca gagtgccgtt tttacgcact
    13081 tcagcctgac gctgtaccag acggtgtgca taggtgatag gtgctggcat cagcacgtcg
    13141 gtttcattag ttgcgtagcc aaacatcaga ccctggtcac ccgcgccctg ttccagcgga
    13201 tcggcacggt caacgccttg gttgatgtca ggagactgtt tgccgatagc gctcagaacc
    13261 gcacaggagt tagcgtcaaa gcccatgtcg gaatgcacat agccaatttc gcgaacggtg
    13321 ttacgggtga tctcttcgat gtctacccag gcgctggtgg tgatttcgcc gccaactaaa
    13381 accatgccgg tttttacgta ggtttcgcaa gcaacgcgtg ctttcggatc ctgttcgagg
    13441 atcgcgtcta aaacggcatc agagatttgg tcagcaattt tgtcaggatg cccttcagag
    13501 acggactcgg acgtaaaaag gtgttttgcc atatttaata tcacctaaag agaatttggt
    13561 tagctcaaac tgttgtgtgg attttctgtg gtagcggatc ctaccacgac tctgcaggtt
    13621 aaaaacactg gcagtctgag tgttaatcgg tatggatgga ttaacatctg gatggctatt
    13681 ttaggtcaat tctt
//
LOCUS       DAJTYH010000098        13514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTYH010000098 DAJTYH010000000
VERSION     DAJTYH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            375..1166
                     /locus_tag="OO609_003070"
     CDS             375..1166
                     /locus_tag="OO609_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634396.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1552..1758
                     /locus_tag="OO609_003071"
     CDS             1552..1758
                     /locus_tag="OO609_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6634397.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1844..2416
                     /locus_tag="OO609_003072"
     CDS             1844..2416
                     /locus_tag="OO609_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634398.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2418..2702)
                     /locus_tag="OO609_003073"
     CDS             complement(2418..2702)
                     /locus_tag="OO609_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634399.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2673..2816
                     /locus_tag="OO609_003074"
                     /pseudo
     CDS             2673..2816
                     /locus_tag="OO609_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3985..5004
                     /locus_tag="OO609_003075"
     CDS             3985..5004
                     /locus_tag="OO609_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6634400.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5114..5986
                     /locus_tag="OO609_003076"
     CDS             5114..5986
                     /locus_tag="OO609_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6634401.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6320..9442
                     /gene="ag43"
                     /locus_tag="OO609_003077"
     CDS             6320..9442
                     /gene="ag43"
                     /locus_tag="OO609_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR6634402.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9563..12079
                     /locus_tag="OO609_003078"
     CDS             9563..12079
                     /locus_tag="OO609_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634403.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12155..12610
                     /locus_tag="OO609_003079"
     CDS             12155..12610
                     /locus_tag="OO609_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634404.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(12729..12940)
                     /locus_tag="OO609_003080"
                     /pseudo
     CDS             complement(12729..12940)
                     /locus_tag="OO609_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13021..>13514
                     /locus_tag="OO609_003081"
     CDS             13021..>13514
                     /locus_tag="OO609_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6634405.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEE"
BASE COUNT         3626 a         3343 c         3333 g         3212 t
ORIGIN      
        1 gattgggtta taaggtgtat ttacaaggta taatcacagg atcatttaca tgaccatact
       61 tctgtaaata atactaatta accttaatta tttttttatg gtacatgaac tacctgtagc
      121 tgtaacgata aacaaatcac ccaaaatgaa aaatactggt tatcacagtt cagagaactc
      181 gtatgagtgt ccccctgagc aatgcttcat tttctgtgat tctgtgtgat agagttatac
      241 tgagtacagt ggcagtaaca ctgctttcca tcacttgagg ataaaattca ttaaaaaatt
      301 aattgcatga attaacaggt ttttttatga aatgttatgc atctcaacca atatgattaa
      361 cagaaaactt catcatgaca acacgtataa cgttcagtat gaccagtgat gagacgctgc
      421 gaattgtgga tgagtactgt catacccaca aactttcgcg ctctaaggta atcaacgcat
      481 tactcagcgc cacagcacct gtccttaatg atattaactg ttattatcaa cttgcaggcg
      541 aactgcagtc acgcttatta aatggagtgt atcaacggga tcttcctcgc aaacgcaatg
      601 tcgtcagtgc agagaagtat tgtctggaaa tctgggaaaa caaacttttc accagaagaa
      661 tcctggagtt tgacagttca aatggtgttc tgtacgcgct gaaacataaa agacactatc
      721 gccgggataa gatgattgga agggttgaaa gtcggtgtat caaagacatt tgcgaatacc
      781 agatgcagct atccggggag aagacgaaat atgcctgttt tatttatatc gaaagaacaa
      841 tatacaatca cgataatcca tctgataaaa tacctgtaaa agcggctgtt ggtaatgcag
      901 tcatcctgct ggcaaaggat gtgatatata acgagtactt ttttgatttg cggcagtcat
      961 tttttgtgag cgttacagat ctgatggtct ctggcgcaaa aggtattccg gagacacaga
     1021 catatccgga cgtttattgc tggatccctc ttttttccat aaattccggc gtgctcatta
     1081 cccctgtata taaaattgac cctctgaaac ccgtaacggt aaaaaaaccg gataagatca
     1141 ctgttgtttg taattacaga gaataatttc agccttctac cttcaccgcg acagtgttta
     1201 cctctgcttt atcttccgtt cagtaaatac aaaccaccgc tctggcaggt acagggcgtg
     1261 gtttatctcc gatactcccc tgtgcagtaa taatcatcac tgaaaatact gatcccgtca
     1321 cggcaaaacg ccatcccata ttccggcaac acaaaatcat cttccgttta taccggattc
     1381 actgacaaaa cagacaacct gtattgcctg tacacaacag tttctctcct ggcaggaata
     1441 ttcccctact cattttttac gtttctcccc ggttgttatg ccggtgaagg tggtgcgtcg
     1501 ttttcatcac caacgccggt tgagattaac aacatccgga ggaacattct catgaatacc
     1561 ccagtttcac tgatggatga ccagatggtc gacatgtcgt ttatcactca gctgaccggc
     1621 ctgaccgata agtggtttta taaactcatc aagcatggag cctttccgcc ccccatcaaa
     1681 ctgggccgta gctcccgctg gctgaaaagc gaagtggaag cctggctgca ggcgcgtatt
     1741 gcacagtccc gtccgtaatt cctgaccgtt atccgttcca ccgcagcaaa ggcatgatcc
     1801 ggcccccgga ggcattttgc tgcgcgctaa aggagagaat attatgcaac aacttgaaaa
     1861 caggtgtgac ctgctgctca tccagcatca gaaatggatg acaagcgtga cgaggcttat
     1921 cgtcgctcat ggaatggggt ctccgcatct tcatggttat caccgtctga ccctggcaca
     1981 cttttttctg ccggaaaaag gctccgtcat ttcggtggcc ccccagggac tctatcaggt
     2041 cgtcaacccg ggaacaccac cgttcattcc tgccattcag gaagggttaa tgaccagcat
     2101 tcagacccat gaaatcatgc tgcttacaca ttttaatctg ggcggcgttt tactcagtga
     2161 gctacatcgt ctgggtgaaa accggctggc aaacaggctg aacagcctgc tccggcgttt
     2221 cgacgatcgt gatctctacc acacgctgat atggctgtgc tggtatgatc tgatgtgtgc
     2281 ccactccatg cagccctgga ctgaagagtt aaagcataaa tcacatgcag agcttgaaaa
     2341 ctgggctgtg gcgcgcaaac gggaaaagcg cgagctggaa ctgatgattg acgagtacct
     2401 gttatacgcc tgctgattca taacgttccc ggggttcctt cctcatcgtt acacacagcc
     2461 ggacggctat ctatggctgt ccgccaaatg accatcgccg catttcgttc cgctgaaaac
     2521 ggtacgtagt cagtgatacc ggaatttccg gagagggccg caggtatatt gcttctgtgc
     2581 aattgatgct tatttacatt ggatgtagtt atttctgcat ccaatgctat cggaaagaga
     2641 ttaaccagca aatggttcct caaatcgtat tgatatttac ccggcgtatt gagcaccacc
     2701 atctgacact ccgttcccgc atcaaacgac tggaccgaaa aacgatttac ttttcacgtg
     2761 ctgtggaaat tcacgaaaaa attattgggt cattcatcga aaaacatatg ttctgatgac
     2821 tggagtcatc ctccagaaat atacgtcaac tgaacaatat cgatttttta tgggattcta
     2881 tacacttttc catatccctc tgaaaatcag tcatcagctc accaacacct cttctgaaac
     2941 ttgtattaaa cttattgctt aaatagatga tataacatct cttccattgc actgaatcgg
     3001 cgaaaactgc cgtttcaata atctcaacaa tattatttat cggctaactt tatcagtaaa
     3061 caatggaatg tcacactgat gaaatgaaca ttcagagtaa gatgataaaa ttcatataaa
     3121 cgccattgct gaatattatt tatttcatac agataaacga taaaatacaa aagggtatat
     3181 ttctcaatgt aacagtcaat tattcatctc cactggcgag cacggtcagc acacaaacaa
     3241 agttagtcct cctgcttaat cattgcgtat cagaaactaa aaaagaaaaa attctgcaga
     3301 caacttaaca atcttcatca catttcctga agacagacat atccacaaac ggtagtcagc
     3361 gttgaattaa caagtcttac agagctatac gtagcttata aagccactca atgttcatca
     3421 tcacccgact acttatctgg aataccacca gtataaactc atcaataaaa acacctgttg
     3481 ttaaaataag aatactgtca gcagtaaatt attaaaacaa catctgtcca cactatctgt
     3541 tcttttaacg ccaacggcag cgaaaatagt gcatcacagc cttctttttt ctttcgccac
     3601 cactcagaca aacacaacac tcacacccac accacaaaac agaatcaaaa cataaaatta
     3661 acaataacag tcaaaaggaa aacacagaat attgaggtgg actgtcacca ggacagcgca
     3721 tacgacgcat gtgatcagca catcccgttc ttcgctggca tccccaaaat gcagaacaaa
     3781 caatcataac attatgaata taaaagaaaa tatgattttt caccatgaaa aaaacatcaa
     3841 tgtatctctg tataaaaata atgataacat gacataaaca tttaaaaaca taaataaaca
     3901 gcaacaaaaa catttgataa tcacttagtg attaaataca attaagacga ttttgttata
     3961 agagaggcac catggagtga gataatgaat aacgaacaga tgcttatcaa atgcagagat
     4021 ccccgtaaat gtcggtttta tgccgaatat ctgaatcaca aataccctaa tgaattccct
     4081 cactttgcgc ctgacaaccc acaaaattgt atcaggaatg ttaatttatt tttaaaggaa
     4141 aagtattgta atgtttcacc tccccccttt ggtacttttg gtactactga agaagaagca
     4201 ctcaataacg aaattaatga gcttgaaatt attgccgggc aggcaatact gcctgaagag
     4261 agttttctct ggttgaaaac tgacgaactt gcgactttct ttacctggac tactatttat
     4321 ctgtcgaagg aagaccatac aaaattcccg aaaataaacc ataattcatc aggaataaaa
     4381 aacggaattg aagtcaggaa aatacgaaca gttaccgatg aaacgttaaa cacttacaaa
     4441 aaaagtaacc ttccggataa tccatcatcc cataacgaac gaataaaagt aatcatttca
     4501 tattttgact cctgccattc tttaattaat aaaaatgcaa aaagcatata cctcgaggaa
     4561 ctcaggcagg cgtggcttaa aaagaaaagc gcttgtcaaa agtttaagtg gctccttcct
     4621 gacgatgatg aaatgtgtca gtggttctgg acaagactga aacaaaaaca ggatgaaaaa
     4681 catccagtac ataacccagt gacccggtgg ttcataccat cttcaacgaa agaatattat
     4741 cttgcctctc ttgtcgcctt tgctttatgg aaagaagcgc ctgataccat tgaactgttc
     4801 agaacacgaa taaatcatgc ctggcatcag aaaaaacaaa aggacaaata tcggagtgat
     4861 ggtaaaaagg ccattaatat acatataaga agcaatgtga aagaaatgct tgatgagctc
     4921 agaactgttt acggtctgag aacaggagag ttttttgaaa tgctgattac agagaaatat
     4981 cttgaacaca agcgaaggaa gtaattcatt acctgttaca ttctaaataa tctcagacag
     5041 atattatctc cgtgaatccc ccgccacctt tccggtgcgc ggggttttgt cttttttcac
     5101 cgggaataca tgtatgaatc cgtctgatgc cattgaggca attgaaaaac cgctctcctc
     5161 cctgccttac tcactttccc gtcacatcct ggaacatctg cgcaaactca ccagtcacga
     5221 acccgtgatt ggcattatgg gtaaaagcgg ggccggtaaa tcttcactct gtaatgcact
     5281 gtttcagggg gaggtcacac cggtcagtga tgttcacgcc ggcacccggg aagtgcagcg
     5341 cttccgtctg agtggtcacg gtcacagcat ggttatcact gacctgcccg gggtgggcga
     5401 gagccgggac agggatgcag agtatgaagc cctgtaccgt gacattctgc ctgaactgga
     5461 cctggtgctg tggctgatta aagccgatga ccgggccctg tctgtggatg agtatttctg
     5521 gcgacacatc ctgcagtgcg gacatcagca ggtgctgttt gtggtgacgc aggctgacaa
     5581 aacggaaccc tgccatgaat gggatatggc aggtattcag ccttctcccg cacaggaaca
     5641 gaacattcgt gagaaaacgg aggcggtatt ccgtctgttc cggcccgtac atccggttgt
     5701 tgccgtatcg gcccgcaccg gctgggaact ggatacgctg gtcagtgcgc tcatgacagc
     5761 gcttcccgac catgccgcca gccccctgat gacccgactg caggacgagc tgcgcacgga
     5821 gtctgtccgc tctcaggccc gtgaacagtt taccggtgcg gtggaccgga tatttgacac
     5881 agcggagagc gtctgtgttg cctctgttgt ccgtacggcc ctgcgcgctg ttcgtgacac
     5941 cgtggtctct gttgcccgcg cggtatggaa ctggatcttc ttctgaacct gttgtggatg
     6001 atgtcctccc tgcctctgag tctgctcaca aaagcgctgt tttcgttact gtctctcttg
     6061 tccgtgcaat agactgataa tagaataaaa cgatcgatac ctattttacc gatcgttaat
     6121 atcgatcgtt atgctaataa taactcctgt tagcaacgtg cgcagataca cacagacatg
     6181 agattcaggg aacaacagag ccacacgtca gaaacttccg tcagctggac ctccggcact
     6241 gtaacccttt acctgccggt atccacatct gtggataccg gcttttttat tcaccctcac
     6301 tctgattaag gaaatgctga tgaaacgaca tctgaatacc tgctacaggc tggtatggaa
     6361 tcacattacg ggcgctttcg tggttgcctc cgaactggcc cgcgcacagg gtaaacgtgg
     6421 cggtgtggcg gttgcactgt ctcttgccgc ggtcacgtca ctcccggtgc tggctgctga
     6481 catcgttgtg cacccgggtg aaacagtgaa tggcggaaca ctggtaaacc atgacaacca
     6541 gtttgtatcc ggaacagctg atggcgtgac tgtcagtacc gggcttgagc tggggccgga
     6601 cagtgacgaa aacaccggcg ggcaatggat aaaagcgggt ggcacaggca gaaacaccac
     6661 tgtcaccgca aatggtcgtc agattgtgca ggcaggagga actgccagtg atacggttat
     6721 tcgtgatggc ggagggcaga gccttaacgg actggcggtg aacaccacgc tggataacag
     6781 aggtgagcag tgggtacacg ggggagggaa agctgccggt acaattatta accaggatgg
     6841 ttaccagacc ataaaacatg gcggactggc aaccggaacc atcgtcaaca ccggtgcaga
     6901 aggtggtccg gagtctgaaa atgtgtccag cggtcagatg gtcggaggga cggctgaatc
     6961 caccaccatc aataaaaatg gccggcaggt tatctggtct tcggggatgg cacgggacac
     7021 cctcatttac gccggtggtg accagacggt acacggagag gcacataaca cccgactgga
     7081 ggggggtaac cagtatgtac acaacggtgg cacggcaaca gagacgctga taaaccgtga
     7141 tggctggcag gtgattaagg aaggaggaac tgccgcgcat accaccatca accagaaagg
     7201 aaagctgcag gtgaatgccg gcggtaaagc gtctgatgtc acccagaaca cgggcggtgc
     7261 actggttacc agtacagctg caaccgtcac cggcacaaac cgcctgggag cattctctgt
     7321 tgtggagggt aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac
     7381 cggacacaca gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg
     7441 tggcaccgcc accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc
     7501 cgctgtcagt ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga
     7561 tgccctgatg ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga
     7621 taccacggta aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac
     7681 gctgaataac ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac
     7741 catccgtgaa ggcgatgcac tcctgcaggg aggcgctctc accggtaacg gcagcgtgga
     7801 aaaatcagga agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa
     7861 cctgaatgaa ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca
     7921 gcgcggtaca gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac
     7981 gaatgtcact ctcgcctctg gtgccacctg gaatattccc gataacgcca cggtgcagtc
     8041 ggtggtggat gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa
     8101 gttcgtaccg gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct
     8161 gcgtgtacgc ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggtggcag
     8221 ggcaaccgga aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc
     8281 gaccagcggt aagggtattc aggtggtgga agccattaac ggtgccacca cggaggaagg
     8341 ggcctttatc caggggaata agctgcaggc cggtgccttt aactactccc tcaaccggga
     8401 cagtgatgag agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta
     8461 tgcctccatg ctgacacagg caatggacta tgaccggatt ctggcaggct cccgcagcca
     8521 tcagaccggt gtaagcggtg aaaataacag cgtccgtctc agcattcagg gcggtcatct
     8581 cggtcacgat aacaacggcg gtattgcccg tggagccacg ccggaaagca gcggcagcta
     8641 tggcttcgtc cgtctggagg gtgacctgct cagaacagag gttgccggta tgtctgtgac
     8701 cgcgggggta tatggtgctg ctggccattc ttccgttgat gttaaggatg atgacggctc
     8761 ccgtgccggc acggtccggg atgatgccgg cagcctgggc ggatacctga atctgataca
     8821 caacgcctcc ggcctgtggg ctgacattgt ggcccaggga acccgccaca gcatgaaagc
     8881 ctcatcggac aataacgact tccgcgtccg gggctggggc tggctgggtt cgctggaaac
     8941 cggtctgccc ttcagtatca ctgacaatct gatgctggag ccgcaactgc agtacacctg
     9001 gcagggactc tccctggatg acggccagga taacgccagt tatgtgaagt tcgggcatgg
     9061 cagtgcacaa catgtgcgtg ccggcttccg tctgggcagc caccacgata tgaactttgg
     9121 taaaggcacc tcatcccgtg acaccctgcg cggcagtgcc aaacacagtg tgcgtgaact
     9181 gccggtgaac tggtgggtac agccttctgt tatccgcacc ttcagctccc ggggggacat
     9241 gagcatgggt acagccgcag ccggcagtaa catgacgttc tcaccgtcac agaatggtac
     9301 gtcactggac ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt
     9361 tcaggccagt tatgcccaca gcatcaacgg cagcagcgct gaaggttata acagtcaggc
     9421 cacactgaat gtaaccttct gataattctg cattgcctct ctgtggtccc ggtcatgatg
     9481 accgggaccc ggacggggga aaacgtttca gtgccacatt cactggcatt cacaataaca
     9541 tgatattcat cacggagtga ctatgttaca gatagtcggc gcgctgattc tgctgatcgc
     9601 aggatttgcc attcttcgcc ttttgttcag agcattaacc agcacagcgt ctgcgctggc
     9661 agggttcata ttgctgtgtc tgttcggtcc ggctttactg gctggctata tcactgaacg
     9721 cataacccgg ttattccata ttcgctggct ggcaggcgta tttctgacga ttgccggaat
     9781 gatcatcagc ttcatgtggg gacttgatgg taaacatatc gcactggagg ctcatacttt
     9841 tgactctgta aaatttattc tgaccaccgc tctcgccgct ggtctgctgg ctcttcccgt
     9901 gcagataaga accattcagc agaacgggct cacacctgaa gatatcagca aggaaattaa
     9961 cgggtattac tgctgttttt atactgcttt tttccttatg gcgtgttctg catacgcacc
    10021 attgatcgca ttgcagttcg atatttcacc ctcactgatg tggtggggcg ggttgttgta
    10081 ctggctggct gcattagtga cgctgctatg ggcggccagc cagatccagg cgctgaaaag
    10141 actgaccagt gccatcagcc agacactgga agaacaaccg gtgctcaaca gtaaatcgtg
    10201 gctgagcagt ttgcaaaacg attacagcct tcctgaaacg ctgacggagc gcatctggct
    10261 gacactcatt tcacaacgga tttcccgggg agaactgagg gaatttgaac tggcagacgg
    10321 aaactggtta ctgaacaatg cctggtatga aagaaacatg gcagggttta acgaacagtt
    10381 gaaagagaac ctgtcattca cacctgatga actgaaaacg ctcttccgga accgcctgaa
    10441 tttatcaccg gaagcgaatg acgattttct cgatcgttgc ctggacggcg gtgactggta
    10501 ccccttttca gaaggccgcc gttttgtatc attccaccac gtggatgagc ttcgtatctg
    10561 tgcctcctgc gggctgacag aagtacatca tgccccggaa aatcataagc cggctccgga
    10621 atggtactgc tcctctcttt gtcacgaaac agaaacactg tgtcaggaca tttatgaacg
    10681 ttcttacacc ggttttattt ccgatgcaac ggcgaatggt ctgattctca tgaaactgcc
    10741 ggaaacctgg agtacaaatg agaaaatgtt tgcttccgga gggcagggac atgggtttgc
    10801 cgctgaacgg ggaaaccata ttgtcgacag agtccgtctg aaaaacgcac ggatcctcgg
    10861 tgataataat gccagaaatg gagcagacag actggtcagc ggaacagaaa tccagacgaa
    10921 atattgttca actgcagccc gtagcgtcgg tgcggcattc gacggacaaa acggacagta
    10981 tcgttacatg ggaaatcatg gtcccatgca actggaagtc ccccgtgatc agtatgccgg
    11041 cgctgtggaa accatgaaga ataagatccg cgaaggtaaa gtacccggtg taaccgatcc
    11101 cgcagaagcg tcccggctga ttcgtcgggg acatctgact tatacccagg cccgtaatat
    11161 cacccggttc gggaccatcg aatcggtcac ttatgatatt gccgaggggt cggttgtcag
    11221 tctggcggcc ggagggatca gttttgccct gacggcatcg gtcttctggc tcagcaccgg
    11281 cgatcgcgat gctgccctgc agacagctgc tgtccaggca ggaaaaacct tcacccgcac
    11341 actggctgtc tacgtcacaa cccagcaact tcaccggctc actgttgttc agggtatgct
    11401 gaagcatatt gatttttcga cggccagccc gactgtccgg caggcgcttc agaaggggac
    11461 cggtgcagga aatatcagtg ccctgaacaa agtgatgaag ggtacgctgg tgacatctct
    11521 ggcactggta gctgtcacaa ccagccctga catgatcaaa atgttgcggg gacggatctc
    11581 cggtgcgcag ttcatcagga atcttgccgt ggcatcttcc ggtgtggcag gtggtgctgt
    11641 cgggtcagtg gcgggcggga tattgttcag tccactggga ccatttggtg cactgacagg
    11701 gcgtgtggtt ggcggtgttc tggggggaat gattgcctcc gctgtatcag gaaaaattgc
    11761 cggagcgctg gttgaagaag atcgcgtcaa aattctggca atgattcagg agcaggtgac
    11821 atggcttgcc ggcagtttcc tgctgaccgg acatgagatt gaaaatctga acgcgaatct
    11881 ggcccgtgtt atcgatcaga atgctctgga gatcattttc gccgccggta tacaacaacg
    11941 ggccgcgacc aatatgttaa tcaaaccact ggtggtcagt atcatcaggc aacgccccgt
    12001 catggaatat gatgcatccc atctcggcaa gatggttaac cggctggaag aagcattccc
    12061 cccggagctc ccggcataac gtgtgttctg ctccttttag gttttatcac ctgatttgcc
    12121 agtgtcatcg tcatgactca gagaggtaaa taccatgatt cacctgttca aaacctgcat
    12181 gattaccgcc ttcattctgg ggttaacgtg gtctgcccca ctccgggcac aggatcaacg
    12241 ttacatcagt atacgcaata cagatacgat atggctcccg ggaaatattt gtgcttacca
    12301 gttccggctg gataatggcg gaaacggtga aggatttggc cccctcacca tcactctgca
    12361 actcaaagac aaatatggtc agacgctggt gaccagaaaa atggaaacgg aagcttttgg
    12421 tgacagtaat gccacgcgaa ccacagacgc atttctggaa acggagtgcg tggaaaatgt
    12481 cgccacaacc gaaatcatta aagcaactga agaaagtaac ggccatcgtg tcagtctgcc
    12541 gttatcggtt ttcgatcccc aggactacca tccactgctg attaccgttt ccggaaaaaa
    12601 tgttaactga tccctgaagt cagtttcatg cttcagcctc tgccacaacg tatggcagag
    12661 gctatttatc aggagaaacc agatatgccg ggttacacac agagtatgtc ctggctgaag
    12721 gatctttctc atacgggcag gcggttgctg tcataaccgc ttaccgcaat gtgtttatcc
    12781 aggatgaccc gggaatgcat ttccgtcggg ttatccgcaa cgcggaaggt cagcgacgct
    12841 gacgcagccg gaactctgaa gctgatgccg gaaaacagct taatgcgtgg ctcgccagtg
    12901 gagggcttct cagacaataa acttcattga tttcatccat caggccgcgt cttctccggg
    12961 agacgcggct tttttcattt gttgcaccag taaatcttaa ccaccgataa ggagcaaagt
    13021 atgcaattag tcagtcgttt tggttatgcg aaccagatac gccgtgaccg tccgctgaca
    13081 catgaagagc tgatgcacca tgtgccgggg attttcgggg aagagaaaca cacgtcccga
    13141 agccagaact atacgtacat ccccaccatc accgtactgg aaagtctgca gcgggaaggc
    13201 ttccagccct ttttcgcctg ccagacccgt gtgcgcgacc cgggccgccg gggatacaca
    13261 aaacacatgc tgcgtctgcg gcgggccgga gagataaacg gagaacatgt ccctgaaatt
    13321 attctgctca actcccatga cggtacctcc agctaccaga tgctgccggg ttacttcaga
    13381 ttcgtctgcc agaacgggtg tgtctgtggt cagtctctgg gggaagtgcg tgttccgcac
    13441 cggggaaacg tggtggagaa agttattgaa ggggcttacg aagtggtggg cgtgtttgac
    13501 cggattgagg agaa
//
LOCUS       DAJTYH010000099        13388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTYH010000099 DAJTYH010000000
VERSION     DAJTYH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..1457)
                     /gene="aldB"
                     /locus_tag="OO609_003082"
     CDS             complement(<1..1457)
                     /gene="aldB"
                     /locus_tag="OO609_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6634406.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHVAFGGYKQSGIGRET"
     gene            complement(1622..2773)
                     /gene="yiaY"
                     /locus_tag="OO609_003083"
     CDS             complement(1622..2773)
                     /gene="yiaY"
                     /locus_tag="OO609_003083"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6634407.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(2963..4807)
                     /gene="selB"
                     /locus_tag="OO609_003084"
     CDS             complement(2963..4807)
                     /gene="selB"
                     /locus_tag="OO609_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6634408.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(4804..6195)
                     /gene="selA"
                     /locus_tag="OO609_003085"
     CDS             complement(4804..6195)
                     /gene="selA"
                     /locus_tag="OO609_003085"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6634409.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(6293..6901)
                     /locus_tag="OO609_003086"
     CDS             complement(6293..6901)
                     /locus_tag="OO609_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6634410.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(6972..8057)
                     /locus_tag="OO609_003087"
     CDS             complement(6972..8057)
                     /locus_tag="OO609_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6634411.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8060..8422)
                     /locus_tag="OO609_003088"
     CDS             complement(8060..8422)
                     /locus_tag="OO609_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6634412.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            8959..10872
                     /gene="mtlA"
                     /locus_tag="OO609_003089"
     CDS             8959..10872
                     /gene="mtlA"
                     /locus_tag="OO609_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6634413.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            10967..12115
                     /gene="mtlD"
                     /locus_tag="OO609_003090"
     CDS             10967..12115
                     /gene="mtlD"
                     /locus_tag="OO609_003090"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6634414.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12115..12702
                     /gene="mtlR"
                     /locus_tag="OO609_003091"
     CDS             12115..12702
                     /gene="mtlR"
                     /locus_tag="OO609_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6634415.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(12714..12923)
                     /locus_tag="OO609_003092"
     CDS             complement(12714..12923)
                     /locus_tag="OO609_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634416.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13207..>13388
                     /locus_tag="OO609_003093"
     CDS             13207..>13388
                     /locus_tag="OO609_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634417.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKL"
BASE COUNT         3223 a         3609 c         3394 g         3162 t
ORIGIN      
        1 gtttcgcgac cgatacctga ttgtttgtag ccaccaaacg caacatgtgc cgggtaagcg
       61 tgataacagt tggtccacac gcgcccagcc tgtatgccgc gccccatctt ataggccaga
      121 ttaccgttgc ggctccagac gcccgcgccc aggccatatt gcgtatcgtt cgccagctcc
      181 agcgcttctt ccatcgtttt gaaggtggtc accgccagca ccgggccaaa aatctcctcc
      241 tggaacaccc gcatattgtt ctgaccaaac agaatcgtcg gttcgaggta gtagccgtct
      301 ttcagttcac cttccagcag cttgcgccgc ccgcctgtga gcacgtcagc gccctctttt
      361 ttaccgatat caatgtagtt gaggatggtt tccagttgcc cgtgagaaac ctgcgcgccc
      421 atttgcgtca cgctgtcgag cgggttaccg ctgcgaatgc tttcgacacg gcggatggcg
      481 cgttccataa agcgttcgta gattgactcc tgcactaagg cacgactcgg acaggtgcaa
      541 acttcgccct ggttaaaggc aaacagtgca aagccttcca gcgctttatc gaaaaaggca
      601 tcttcttcat ccatcacatc agcaaagaag atatttggcg acttaccgcc caactccagc
      661 gtcaccggaa taatgttttg cgttgcgtat tgcataattt gttggcccac ttccgttgag
      721 ccggtaaacg ccactttggc gatgcgtttc gaggtcgcca gatattcgcc aatttcccca
      781 cctgcgccat tgaccacgtt caccacgccc ggcggcagta aatcaccgac aatttccatt
      841 agcagcagta cagaaagcgg ggtaagacgt gcgggtttca gcaccacaca gttgcccgcc
      901 gccagcgcgg gagccatttt ccagctcgcc atcagcagcg ggaagttcca cgggataatc
      961 tgccccacca cgcctaacgg ttcatggaaa tgataggcca cggtttcgct atcaacttca
     1021 ctgatcccac cttcctgcgc ccgaatacac gaggcgaaat agcggaaatg gtcaatcgcc
     1081 agcggtacat ccgcagcact ggtttcgcga atgggtttgc cgttatccca ggtttcagct
     1141 gtcgctaaca gctcgaggtt ttgttccatt cgatcggcaa tcttaaacag aatcgccgcg
     1201 cgatcctgca ccgaggtgtg cgcccattta tctttcactt tgtgcgcagc atccagcgcc
     1261 agatcgatgt ctcgtttgcc cgaagacgcc acttcgcaca gcagctgccc ggtcaccggc
     1321 gtcagattct ggtaatactc accatcggca ggggctaccc attcaccgcc aataaagttg
     1381 tcgtagcggg cttttaactt gatggggaaa ccatactcgc cgggcttaat ctgtgctgaa
     1441 gggggattat tggtcatgat cgtctccttg cggtgtgagg tataacaagg gtagacgtga
     1501 ctggcgaaat cttcgccagt cggtaacagc tttacgacag ctatcacgaa tttacgggca
     1561 aggattacag agatagttga aaaagcggct gacaatttgc cagccgttgt tggaatgatg
     1621 attacattgc ggcgcgataa atcaccacaa tttcttcgtg agttgcctgg atcgggttag
     1681 taaagccaca ggcatctttc agggcattag tcgccagaac cgcgaaatct tcttctttca
     1741 cgttcaggtc gcgtaggcct gccgggatat ccactttctt cgccagttca cggatggcgt
     1801 taatgcaggc ttcagcacct tccgcgtcgt ttttacctgt cacgttcacg cccattgcag
     1861 cggcacagtc acgcagacgt gcagcggcga ctttgctgtt gaatacctga acatgcggca
     1921 gcagaacggc gttacataca ccgtgtggca ggttgtagaa accgcccagc tggtgcgcca
     1981 tcgcatgaac ataacccaga gaagcattat tgaacgccat accggcaagg aactgggcat
     2041 aagccattgc ttcacgcgct ttcgcattac tgccatcttc aacggctaac ggcaggtttt
     2101 cggcaatcat ggtcacggct ttcagtgcac aagcgtcagt gatcggcgtg gcggcaatag
     2161 aaacatacgc ttcgatagcg tgcgttaagg catccatacc cgttgcggcg gtcagtgact
     2221 tcggcatacc aatcatcaga gaggagtcat tgacagaaag cagcggagtc acgtgcttat
     2281 caacaatcgc cattttgtta tgacgcgctt cgtcagtgat gatgcagaaa cgggtcattt
     2341 cagaggccgt acccgccgtg gtattgatgg cgatcatcgg cagctgcggt tttgcagagc
     2401 ggtcaacgcc ttcgtaatcg cgaatatcgc cgccattggc tgccaccagc gcaatacctt
     2461 ttgcgcagtc gtgtggagaa ccaccgccta aggagatcac gctatcgcaa ttattttctt
     2521 taagtaattt caaacctgcg gcgacgtttt ccgtggtggg gttaggttgg gtgccatcat
     2581 aaataacgct aaaaatattg cgttcttcca gtgctttttg cacatcaccc gccataccta
     2641 atttcgttaa catattgtca gtgacaatta aggtacgggt aaatccataa tctgccatca
     2701 tattcattgc atcagtcaat gaatcagcgc cgatgacatt cacagaagga ataaagaaag
     2761 ttgaagatgc cataacacac tcctgaaagt gtaaaaaagg gtgggcagct tactgaggat
     2821 tttcattcga aaacatgatc attattatta attatcaaaa ttaatctaat aaaaagcaca
     2881 ttgtattcat taaacaatgt gctttattta aaatcataag aaatttgtat taatttattt
     2941 taaaaattaa ttaatcattt ccttattttt ccggaaataa taatgcgtcg cgcaataaat
     3001 gatcatttcc acgacgacgc gtaaagccaa tgcggtcaaa atattccaga atctgaattg
     3061 ccagctttcg gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt gaaccacact
     3121 cctgatcgag atcgcggatc atattggcaa attcgacaat ccgatcgtta cggtaataac
     3181 gatctttgac gatcgcggta attattcctt gctgcgccgc ctggcgtagc gtcaggcgca
     3241 ttgcctgctc gtcggtaccc gtctcttttg ccagatcacg cacccaccac ggctcgtcac
     3301 caaacagcgg ctctgctttt tgccagatgg cctgctgctc ttcgctgaag cccgctttgt
     3361 gatccggcag atgcagccaa ccgtgatgac tgaggatgtc gccgctttcg cgcatctttt
     3421 caatcagcaa cagtactagc gcttcatctt ccattggcaa cgccatacgt cgcagacgtt
     3481 cgcgcccagg cccaggttca tcgcgatgtt gctcatgata agtcgctaat gtgtcgagaa
     3541 ttttccgttg ccagcgagcg gcaaccggcg cattcaacaa gctataacca gcctgaatat
     3601 agccaggctg ttgcagcagt tcgcgcatcc cttcgccgtt gagctggcgc gcccaagcga
     3661 aatcaggaag attaaccgcg ccgcgttgca ggtgaacggc taacgcttcg ctatcattct
     3721 gtgccctggc gagggaagcc agccattgca gatactctgg tttacgttta ccgcgacgcg
     3781 gcgggttaag catcacgacg cgcgctccgg ccagcgtgtt gcgggcagag atatcgcgca
     3841 ataccaggcg gtcgttatct gccagccata acggggtgtc gaagaccagt tcagcaaggt
     3901 tatcttccag cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga atatgcagcg
     3961 gctgccactg ggtcagcggt gcatgggttt gaagctcgac aatcacccgt gtgaacggct
     4021 ctggcggcac atcggcaagc agccagtcgc cacggttaat ctgctctttt tccgcatcac
     4081 ccgcgatgtc aagcgcgata cgctgcccgg cattggcggt ttctgttggc tggttttgcg
     4141 catgcagcgc acgtacacgc atcggtttat ttacaccagt cagccagagt gaatcgccta
     4201 ccttcacttc cccgcttaac gccgtaccgg tgacgaccag cccggcacct tttacggtaa
     4261 atgcgcggtc aatcgcgagg cggaaactat gttggctggc gtgctcgcgt tccggcaact
     4321 gaagcagatg ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg gtgataaaca
     4381 gttttgcctc agcaaaaccg tattcccgca gaacctcctt tacctggcgt tcaacctcat
     4441 caacacgcgc ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc atcgggttac
     4501 cggtcagctg caaaatcgcc agatgctcac gggtctgtgc catcacgccg tcatcgcacg
     4561 ccaccaccaa cagcgcgtga tcgataccac caacgcccgc cagcatgttg gaaagaaact
     4621 tttcatgacc gggaacgtcg ataaaaccag gcacgcgacc atccggctgc ggccagtagg
     4681 catagccgag atcgatggtc atgccgcgct ttttttcttc cggcagacgg tcagcattta
     4741 cgccagtaat cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg gcagtcgcaa
     4801 taatcatttc aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc gtaaatccag
     4861 ccacaatcgt ccgtcataaa tacgaccaat caccggcact ggcaattcac gccagcgggc
     4921 ggctaatgac tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta atgccgcgct
     4981 cggcaggcga tcaaccggca gcgaaccact gccaatctgc gaaagacatg gcataacctg
     5041 taccgcaaac tccgcgccgt aatgtgcgac aaggggagcc tgtaaacgtt gtgcctggat
     5101 ttgaatgacc tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt cactcagagc
     5161 ttcagggtgt aaataaagac gcaacgtggc ttccagcgcc gcgagggtca ttttatccgc
     5221 gcgtaatgca cgcttcagcg ggtggctttg caggcgggcg atcatctctt ttttaccaac
     5281 gataatcccg gcctgcggcc cgcctaacaa cttgtcgccg gagaagctca ccagactgac
     5341 gcccgccgca atcaactcct gcggcattgg ctctttcggc aaaccgtact ggctaagatc
     5401 gaccagcgag ccactgccta aatcagtcac cactggaaca tccagctctt tgccgagcgc
     5461 caccagttca gcttcgtcta tcgctttggt gaacccctga atgctgtagt tactggtatg
     5521 tactttcatc aacagtgcgg tattttcatt caccgcctga cgataatcat tcgcgtgcgt
     5581 gcggttggtg gtccctactt cgtgtagggt gcagcctgcc tgacgcataa catcgggaat
     5641 acgaaacgcg ccgccaatct ccaccagttc gccgcgagat accaccacct ctttcccgct
     5701 ggcagtggcc gccaacatca ataacaccgc cgccgcattg ttattgacga tacaggcatc
     5761 ttccgccccc gtaatacggc acagcagctg cgccagcgcc cgatcgcgat gtccgcgtcc
     5821 ggcgtcgtcc agatcgtact cgagggtcac tggcgaacgc atagcctgcg caacggcttc
     5881 caccgcggct tccgcctgta aagctcgccc aaggttggta tgcagcacgg ttcccgtcag
     5941 gttgatcacc ggacgcagcg cgctctgcgc ctctttcgtc aaccgggcat cgacttcttg
     6001 cgcccagttt tcacaccacg caggcagcgt ctggctgcca cgaatcactt ctcgcgcttc
     6061 gtcgagcatc tgacgcaaca attccaccac gcgggtgtga ccataagtat cacgcaaaga
     6121 aaggaaggag ctatcgcgca ataagcgatc aatagccgga agttgactat agagggaacg
     6181 cgtttcggtt gtcataggaa acctggctga tcaaggccct ctcacacgga gaagggcgtt
     6241 taacataacc acggattgta acgtgagatg ggtcaggagg acatatcgcg catcaagcct
     6301 ttggcggttc ggtgcgagca aaactttcgc ggctaaacag gttttcgacc agtttgacta
     6361 agtgtgggcg gtcgacacac caacctggcg caacgcggcg gaaattgaga tatccgacag
     6421 cacaggcaat ggcgatagtc gccagattga ccgtatcggt tttgagtgtg ccatcgacca
     6481 gatatccttc cagcacatcc agactgcggt tgattttctc ccgctggcgt aacaattcat
     6541 cttcagactg ctgcgccgcc ggacgcgcct gttcacgcac cgataccagc ccggcatcca
     6601 taatgccatc cgccagcgcc tcaattttgc gcacccgcaa cgactccagc ggatcgcgcg
     6661 gcaacatcgc cggagcgaca ttcattaact caatatattc agcgatgatc ggcgaatcaa
     6721 accagcattc gccctcttcg gtcaccagca ccggcacttt ccctaacggg ttaaattgcg
     6781 ccacgccgtt atccgcgtta tagggcagtt cattaataaa ttcgaaagtt atgccctttt
     6841 ctaacaacag aataaaaagt ttgcgtacaa acgggctggt gtagctaccg acgagtttca
     6901 tgccgagtcc tttgtgcgag gaaaaatatc agtatggctc aggctcacgc aaaacacagg
     6961 tgatataagt ctcaatgatc caaataaaga taatgcatcc aactggtcat tcttagcagc
     7021 actttccgca tcaccgaaac atggctgaaa tggtcggagt aaaccgccac ctgggcgtag
     7081 atgccgtcgg gtaaggcatc gatatcatcg ttagggtcca gttcaatggt tcccagcaca
     7141 ccgtccgtgc cgggaacgac cgttaatgat tgcaataccc cctgcgcctg ataagaaccg
     7201 cctggcacga caggtaaaat actagtcaat ttgccgtgga acacctgccc aggtagcgcg
     7261 ttaaacacca cttccgcatc atcgccaggt ttcagacgta acagcgagtt ttgccgaaat
     7321 tgggcgacaa tttgccgttt ttgctcgggg atgaagacca tcaccggacg cagcggcaag
     7381 gcagctgcgt atgtacctgg gcggatcagt acctgagtga cgtagccatt gcttggtgcg
     7441 cgaatgacag tctgctcaag attatatttt gcttcagtaa gttgcgctct taagctcaca
     7501 atctgcgatt gctcgccgtt gaccatactg tcgagctgac tctggatctg cgcctgctcc
     7561 gccaccgagc ctttcactaa cgcatcctgc gcgaggaaat tttgccgcgc atcgtcgatg
     7621 tcacgttccg agaacggatt caccgccgcc tggctgcctt tcaggtaacg ttgataattt
     7681 ttaaacagac ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc ttcagtgagc
     7741 tgcgcacgca gcgtctttat attatgcgtc gccgtcatca ggtcagcctg aagtctgtca
     7801 actcgcgcct ggtaacgaac agggtcgagc ttaaaaagca cctcgccctt ttgaataagc
     7861 tgattattct tgtcagtgac ttcagtaaca attcccgtca cctgtggcgt gatagggatc
     7921 gctatcactg ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat caacaaaatc
     7981 aaaccactca ccagaaacac gcctcccagc gccgccgtcg ccagcgccac gtaagttaaa
     8041 acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg
     8101 cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccggttcag
     8161 tcgctttctc ctgcgacgca acatgtgact gcatacccca gccacgctcc ggttgataga
     8221 gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc
     8281 ccgccgtatg aatagcgtcg gcatgggggt gattgcgctt tttggcaatc agataaggga
     8341 tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca
     8401 acgcgaaata gtttagaaac atatctgcct cggattcacg tttatcagtg ttgttttttg
     8461 gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc
     8521 gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt
     8581 atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg
     8641 tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacat tactcacatt
     8701 taaatgccat ttttagcgaa aatcaccgcc ttgtttcttt tttatacagg cgttttgtga
     8761 tgagcgtcac gtcaattacc tctctacccc ctatatttat gtgactgata tcacacaaaa
     8821 gcccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg
     8881 gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac
     8941 ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt
     9001 ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc
     9061 gcgttattta ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg
     9121 atgatcactt atctcctacc gctgctgatc ggttataccg gtggtaagct ggtaggcggc
     9181 aaacgtggtg gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg cgcagacatg
     9241 ccgatgttcc tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc
     9301 gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc
     9361 gcaggcatca tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt
     9421 gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg
     9481 ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac
     9541 cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa atcaatcttc
     9601 ttcctgattg aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt
     9661 ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt
     9721 atccacgaaa tctacttccc gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc
     9781 ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg
     9841 gcatctccgg gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttatttcgct
     9901 aacatcgcgg gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc tattttgctg
     9961 aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac
    10021 atgaaagctg agtctaaagg cacatctccg ctatctgcgg gcgatgtgac taacgacctg
    10081 agccacgtac gtaaaatcat cgttgcctgt gacgccggta tgggttcaag tgcgatgggc
    10141 gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc ggtcactaac
    10201 agcgcgatca acaacctgcc gccagatgtg gacctcgtca tcactcaccg tgacctgacc
    10261 gaacgcgcta tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac
    10321 agcggcctgt acaccagcct aaccgaacgt ctcgttgctg cccagcgcca tactgaaaac
    10381 gaagtgaaag tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc
    10441 aaactgggcg cggagaacat cttcctcggt cgcaaagcag caaccaaaga agaagcgatt
    10501 cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata cgttcaggcg
    10561 atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac
    10621 ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg
    10681 gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgca
    10741 gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag
    10801 tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca
    10861 ggtcgtaagt aatccaatcc taccctctcc tcacggagag ggtttgggtg agggaaaagc
    10921 ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca
    10981 ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat
    11041 ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag
    11101 ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc
    11161 tgtcagcagc attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac
    11221 cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa
    11281 acgcaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg
    11341 cggcaccacg cagctgaaag gtcatgtgat gaacgccctg ccggaagacg ccaaagcgtg
    11401 ggtagaagag cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc
    11461 ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga
    11521 taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct
    11581 gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta
    11641 cctcggaaaa ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg
    11701 cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt
    11761 tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta
    11821 tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga
    11881 ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca agaatctgat
    11941 tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccgcagg ctcaggaact
    12001 ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga
    12061 tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg
    12121 gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg
    12181 gaacaaaccc aggccgttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg
    12241 cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt
    12301 caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc
    12361 gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt
    12421 aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga gctaaatcac
    12481 gacggcaacg agtacgcctt taccgacgac gaaatccttg gaccctttgg tgaactgcac
    12541 tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca
    12601 atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag
    12661 ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc
    12721 ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac
    12781 ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga
    12841 tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga
    12901 acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc
    12961 ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc
    13021 ctcaataatc atccgctgct gtttattatc tttttctata gatttcttaa ttaatcaacg
    13081 aattgcgtag tttttgagca tgttaatgat tacgttgcag aactattaca atgtcatcag
    13141 ttgtgaacaa agcacctggt cgcgcatact aggggctata aatttatctt tatcagaagc
    13201 catcacatga aagaagtcga aaaaaacgaa atcaagcgtc tcagcgatcg cctggacgcc
    13261 atccgccacc agcaggccga tctgtcgctg gttgaagccg cagacaaata tgccgagctg
    13321 gaaaaagaga aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt tcatagccaa
    13381 aaactgag
//
LOCUS       DAJTYH010000100        12841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTYH010000100 DAJTYH010000000
VERSION     DAJTYH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(5..1066)
                     /locus_tag="OO609_003094"
     CDS             complement(5..1066)
                     /locus_tag="OO609_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6634418.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1279..2742
                     /gene="bepA"
                     /locus_tag="OO609_003095"
     CDS             1279..2742
                     /gene="bepA"
                     /locus_tag="OO609_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6634419.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            2763..3122
                     /gene="arsC"
                     /locus_tag="OO609_003096"
     CDS             2763..3122
                     /gene="arsC"
                     /locus_tag="OO609_003096"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6634420.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3255..4001)
                     /gene="hda"
                     /locus_tag="OO609_003097"
     CDS             complement(3255..4001)
                     /gene="hda"
                     /locus_tag="OO609_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6634421.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4051..5340)
                     /gene="uraA"
                     /locus_tag="OO609_003098"
     CDS             complement(4051..5340)
                     /gene="uraA"
                     /locus_tag="OO609_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6634422.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5426..6052)
                     /gene="upp"
                     /locus_tag="OO609_003099"
     CDS             complement(5426..6052)
                     /gene="upp"
                     /locus_tag="OO609_003099"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6634423.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6376..7413
                     /gene="purM"
                     /locus_tag="OO609_003100"
     CDS             6376..7413
                     /gene="purM"
                     /locus_tag="OO609_003100"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6634424.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7413..8051
                     /gene="purN"
                     /locus_tag="OO609_003101"
     CDS             7413..8051
                     /gene="purN"
                     /locus_tag="OO609_003101"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6634425.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8223..10289
                     /gene="ppk1"
                     /locus_tag="OO609_003102"
     CDS             8223..10289
                     /gene="ppk1"
                     /locus_tag="OO609_003102"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6634426.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10294..11835
                     /gene="ppx"
                     /locus_tag="OO609_003103"
     CDS             10294..11835
                     /gene="ppx"
                     /locus_tag="OO609_003103"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6634427.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(11874..>12841)
                     /gene="pdeF"
                     /locus_tag="OO609_003104"
     CDS             complement(11874..>12841)
                     /gene="pdeF"
                     /locus_tag="OO609_003104"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6634428.1"
                     /translation="QFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSI
                     VTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEIL
                     LRMKGENDELIGPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLS
                     PTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIA
                     IDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVA
                     EYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         3323 a         3335 c         3234 g         2949 t
ORIGIN      
        1 aagattattc ttgcgcgatt tgcccatcgg gccaggcgtg gatcacggct ttgatcagcg
       61 tcgccaatgg aatggcgaag aatacgcccc agaatcccca caaaccaccg aagatcacca
      121 ccgataaaat aatcaccagc ggatgcaggt taaccgcttc ggagaacaac accggtacta
      181 gcaggttgcc gtccagcgcc tgaataatca gatacaccgc gaagcagctc cagaattcag
      241 tacctgcgcc aaactggaat agcgccacgc caaccaccgg aatggtcacc acaaatgcgc
      301 caatgtacgg aataagaacc gagaagccga ccagcactgc cagcagcagc gaatagttca
      361 gcccgaagag caagaacccc agccaggtgg cgatccccac cacgatcatc tccagcactt
      421 tgccgcggat atagttggtg atttgttgat tcatctcctt ccacacctgt cctgccagcc
      481 cacggttacg cggcagcacc cggcgaacgg cgttcagcat ctgctctttg tctttcagca
      541 ggaagaagac catcaatggc accagcacca gatagacggc tatggtcagc aaaccgacca
      601 gcgaggcgag ggaaattttc accaccgaat cgcccatggt cagcatccga ctgcgcatat
      661 tttcggccat tgcatcaata atgcccgcgt ccattaacac cggatagcgg cgcggcaacg
      721 tggcggcaaa gtcagaaagc ttattgagca tccccggcat atcgcggatt aagtagatgc
      781 cctgttgcca ggcgataggc agtaccacga acgccatcag tagcaatata ccgacaaaaa
      841 ccaccaatac aatcgacgtc gcccagcggc gggagcagcc aatagattgc aggcgcacgg
      901 ttggccattc cagcaaataa gccagcacaa tagccaccag caacggagca agcaggccac
      961 taaagaaaaa gataatgcca aatccggcaa ctaaaataac cagcaaggca atcgcttccg
     1021 ggtcgctaaa acggcggcga taccattgca tcaacatttc gagcataaag accttccctg
     1081 aacctcaaga gcgggattgc gatccgcaat tgtatcgaaa tgtcacaaaa aagacttcgc
     1141 tttttatgac ggattcagga aactgaaaag tcatttgagt gggctaatct tcgccgttac
     1201 actcaaaggc ggcgaggtgg gaacgatatt tcacagtatc ggtcaaatga ctatcttcag
     1261 aaatacagga tagaggttat gttcaggcag ttgaaaaaaa acctggttgc aaccctcatt
     1321 gctgctatga ccattggtca ggtagccccg gcgtttgccg acagcgcaga caccttgccg
     1381 gatatgggaa cctccgcagg aagcacgctt tccattggtc aggaaatgca gatgggcgac
     1441 tattatgtcc gccagctacg cggcagcgcg ccgttaatta atgacccgct gttaacgcaa
     1501 tatattaatt cgctggggat gcgtctggtt tcgcatgcca attcggttaa gacaccgttt
     1561 catttttttc tgatcaacaa cgacgaaatt aacgcctttg ctttctttgg cggcaacgtg
     1621 gtgctgcact ctgccctgtt ccgttattcc gataacgaaa gtcaactggc ttcagttatg
     1681 gcgcacgaaa tctcccacgt cacccaacgt cacctggcgc gagcgatgga agatcaacaa
     1741 cgcagcgcgc cgctgacctg ggtcggcgcg ttaggttcta ttttactggc gatggccagt
     1801 ccgcaggcgg ggatggcggc gctgaccggt acactggcgg gaacgcgcca ggggatgatc
     1861 agtttcaccc agcaaaatga acaggaagcg gaccgcattg gtattcaggt gctgcaacgc
     1921 tcgggattcg atccacaggc gatgccgacc ttcctggaaa aattgctcga tcaggcgcgt
     1981 tactcctcgc gcccgccgga aattttattg actcacccgt tgccggaaag tcgtctggca
     2041 gatgcccgca accgtgctaa tcagatgcgc ccgatggtgg tgcagtcgtc ggaagatttc
     2101 tatctggcga aagcgcgcac actggggatg tataattccg gacgtaacca gctcaccagt
     2161 gatttgctgg atgaatgggc gaaaggaaac gttcgtcagc aacgagcggc gcaatatggt
     2221 cgtgctttac aggcgatgga agccaataaa tacgacgagg cgcgtaaaac gctgcaaccg
     2281 ttactggcgg cagaacctgg caacgcatgg tatctcgatc tggctaccga tatcgatctt
     2341 gggcaaaaca aagccaatga ggcaatcaat cgcctgaaaa atgcccgcga tttacgcacc
     2401 aatccggtgt tgcagctcaa cctggcgaac gcttatctgc aaggcggtca accacaagaa
     2461 gcggccaata ttctgaatcg ctacaccttt aataataaag atgacagcaa cggctgggat
     2521 ttgctggcac aggcggaagc cgcgctaaat aaccgcgatc aggagctggc tgcgcgagca
     2581 gaaggttatg cgctcgccgg acgactcgat caggccattt cgctgttgag tagcgccagt
     2641 tcgcaggtga aattaggcag cctgcaacaa gcgcgttacg atgcgcgcat cgaccagttg
     2701 cgccagctgc agaaacgctt taagccttat accaagatgt aattcgcatt aaaggaagaa
     2761 caatgaccaa acaggtaaaa atctaccata acccacgctg ttcaaagagc cgggaaacgc
     2821 tgaacctgct gaaagaaaac ggcgtggagc cagaagtggt gctctacctt gagacacccg
     2881 ccgatgcggc aacgctgcgc gatttgctga aaatgctggg gatgaatagc gcccgtgaat
     2941 tgatgcgcca gaaagaggat ctttataaag aactaaacct ggctgacagt tcgcttagcg
     3001 aagaggcgct gattcaggca atggtggata atccgaagct gatggagcgc ccgattatgg
     3061 tcgcgaatgg caaagcgcgg attggtcgtc caccggagca ggtactggaa atcgtgggtt
     3121 aagatgtgct ccatcgctag ttgaagcaca ttgccggatg cgacgcttgc cacgtcttat
     3181 ccggcctacg aatactatgg attaacttcg cgtaggtcag gcgttcgcgc cgcatccgac
     3241 aataaacacc ttatctacaa cttcagaatt tctttcacaa acggaatggt cagcttacgt
     3301 tgcgcggtaa tcgacgcacg atccaactga tccaacgtca taaatagcgt gcgcatttct
     3361 ctgtcgagcc gcttcagcaa gaaacgcccc acatcttccg gcagttcaaa accacgcaaa
     3421 cgcgcgcgta actgtagcgc ctgcaactta tcttcatcag aaagtggctg caatttgtag
     3481 atctgccccc agtcgagacg cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt
     3541 ggacgatcgc cggtgatcaa caaccgtgtt ttgcccgatt ccagaattcg attgtagaga
     3601 tcgaaaatcg ccatctccca caactcatcg cctgcaatac actcaatgtt gtcgatacag
     3661 accagcgaca aatgctccat accgtcgagc acttccggaa caaaccaggt gcgtttatcc
     3721 agcgggacat agcccaccgc atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc
     3781 agatggctgc gccccgcgcc ttcgcgtgcc cagagataga tgtaaccgct atgttcctga
     3841 cgcagcacgt tttgcagcgc ggccagtaaa gaggagttat cccccggcca gaaacttgca
     3901 aaggtttcgt cgtcaggaag ataaagtggc aaagagagct gtgccggtgt gttcagagat
     3961 acctcaacca ggatttcaca aaatcgcgag aagtttacca cagaatccca tgatgtttga
     4021 accgggcagc aacactgccc ggtcggtaca ttatttgtct gttatatccg cgtcttctgc
     4081 gtccagcacc acttcttcag ggcgcagcac actgatcagt ttgaaaatca aacttaagcc
     4141 gataccgacg atggtcgcca gcgccatacc ttttaactcc gccgcgccga tgtttacctt
     4201 cgcgccactg acgccgatga tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt
     4261 gtaatccact ttcgattcga tcaaaacacg aataccggaa gcaccaatga caccataaag
     4321 cagcagcgaa acgccgccca tcaccggcaa tgggatcatc tggatagcgg cagccagttt
     4381 accgacgcag gaaagcagga tagcgaaaat cgccgccccg ccgataaccc aggtactgta
     4441 aacacgggtg atcgccatca cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc
     4501 aaagaagccg gagatcacgg tcgacaggcc gttagcaaac atcgaacggt gcagacctgg
     4561 atcgcgcagc agatcttttt tgacgatatt agccgttact accaggtgcc ctacgtgttc
     4621 ggcaataacc actaacgccg ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg
     4681 cgtatagagg gttggcagtg caaaccagtg agcattaata atcggcgtgg tatcgacaat
     4741 tcccattgcg aaagagagcg cgtaccccac cagcacgcca attaaaatcg ggataattgc
     4801 caggaaacca cgaaacagca cggaacctaa aaccgtgacc gccagggtgg taatagagat
     4861 gatgatggtt ttggagtttg gcgtttgccc ttcagccggg agtaaacccg ccataccggc
     4921 agctacgccc gccagctcca gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg
     4981 aaacagcacg tccaaccagc cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa
     5041 cagcacgccg cacataataa agccgcccag cgcgacttca taccctaacg gcaacagtaa
     5101 caataccggt gaaataaagg caaagctgga accaagataa gccggaattt tccctttaca
     5161 gatgaagaga tacagcagcg ttccaatacc gttaaataac agtacagtcg ccgggttaat
     5221 atgaaataag acgggcacca ggacggttgc accaaacatg gcgaacaaat gttgcaaact
     5281 aagcgggatt gtctgtaaaa gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat
     5341 agtattatcc tctgtattat gtgttatagg cgctttactc aaaaaaaagc cgactcttaa
     5401 agtcggcttt aattattttt attctttatt tcgtaccaaa gattttgtca ccggcatcgc
     5461 cgaggcccgg aataatgtat ccgtgctcgt tcagtccctg gtcaatcgat gcagtataca
     5521 gttcgacgtc cgggtgcgct ttttccagcg cagcgatacc ttctggcgca gcgaccagca
     5581 ccagaacttt gatgctgctg cagcccgctt ttttcagcag gtcgatggtc gcgataacgg
     5641 aaccaccggt tgccagcatt gggtcaacga tcagcgccat acgctcatcg atgttagaaa
     5701 ccagtttctg gaagtacggt accggctcca gcgtttcttc attacggtac ataccgacaa
     5761 cgctgatgcg cgcgctcgga acgttttcca gcacaccgtc catcatacca agacccgcac
     5821 gcagaattgg cacaacggta attttcttac ctttgatctg gtcgatttct accgggccgt
     5881 tccagccttc gatagttact ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca
     5941 ggctacccac ttcggaagcg agttcgcgaa agcgcttggt gctgatatct tgctcacgca
     6001 tcagtcccag cttgtgtttg acgagtgggt gtttgacttc cacgatcttc atactctttc
     6061 tcctttgagg ggcagccaca aaaaaatcga cggattatac ctcctttctt cagggcggca
     6121 atattatttt cgttgacttt agtcaaaatg ataacagttt gagataaagt tattttatat
     6181 tcaaatggtt atgaaagaag attattccat ccgaaaacta acctttgccc tggcacaagt
     6241 cttctttcgc cgcgcgcccg gggaaaagac gtgcaaaaag gttgtgtaaa acagtctcgc
     6301 aaacgtttgc tttccctgtt agaattgcgc cgaattttat ttttctaccg caagtaacgc
     6361 gtggggaccc aagcagtgac cgataaaacc tctcttagct acaaagatgc cggtgttgat
     6421 attgacgcgg gtaatgctct ggttggaaga atcaaaggcg tagtgaagaa aacgcgtcgt
     6481 ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt gtgcattgcc gcaaaaatat
     6541 cgtgaacccg tgctggtttc cggcactgac ggcgtaggta ccaagctgcg tctggcaatg
     6601 gacttaaaac gtcacgacac cattggtatt gatctggtcg ccatgtgcgt taatgacctg
     6661 gtggtgcaag gtgcggaacc gctgtttttc ctcgactatt acgcaaccgg aaaactggat
     6721 gttgataccg cttcagcggt gatcagcggc atcgcggaag gttgtctgca atcgggctgt
     6781 tctctggtgg gtggcgaaac ggcagaaatg ccggggatgt atcacggtga agattacgat
     6841 gtcgcgggtt tctgcgtggg cgtggtagaa aaatcagaaa tcatcgacgg ctctaaagtc
     6901 agcgacggcg atgtgctgat tgcactcggt tccagcggtc cgcactcgaa cggctattcg
     6961 ctggtgcgca aaattcttga agtcagcggt tgtgatccgc aaaccaccga acttgatggt
     7021 aagccattag ccgatcatct gctggcaccg acccgcattt acgtgaagtc agtgctggag
     7081 ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga ccggcggcgg cttctgggaa
     7141 aacattccgc gcgtattgcc agataatacc caggcagtga ttgatgaatc ttcctggcag
     7201 tggccggaag tgttcaactg gctgcaaacg gcaggtaacg ttgagcgcca tgaaatgtat
     7261 cgcaccttca actgcggcgt cgggatgatt atcgccctgc ctgctccgga agtggacaaa
     7321 gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga aaatcggtat catcaaagcc
     7381 tctgattccg aacaacgcgt ggttatcgaa taatgaatat tgtggtgctt atttccggca
     7441 acggaagtaa tttacaggca attattgacg cctgtaaaac caacaaaatt aaaggcaccg
     7501 tacgggcagt tttcagcaat aaggccgacg cgttcggcct tgaacgcgcc cgccaggcgg
     7561 gtattgcaac gcatacgctc atcgccagcg cgtttgacag tcgtgaagcc tatgaccggg
     7621 agttgattca tgaaatcgac atgtacgcac ccgatgtggt cgtgctggct ggttttatgc
     7681 gcattctcag cccggcgttt gtctcccact atgccgggcg tttgctgaac attcaccctt
     7741 ctctgctgcc gaaatatccc ggattacaca cccatcgtca ggcgctggaa aatggcgatg
     7801 aagagcacgg tacatcggtg catttcgtca ccgatgaact ggacggtggc ccggttattt
     7861 tacaggcgaa agtcccggta tttgctggtg atacggaaga tgacatcacc gcccgcgtgc
     7921 aaacccagga acacgctatt tatccactgg tgattagctg gtttgccgat ggtcgtctga
     7981 aaatgcacga aaacgccgcg tggctggatg gtcaacgtct gccgccgcag ggctacgctg
     8041 ccgacgagta atgcccccgt agttaaagcg ccagctctgc cgctggcgtt tttcaattca
     8101 cctgtaaatc gcaagctcca gcagtttttt tccccctttt ctggcatagt tggacatctg
     8161 ccaatattgc tcgccataat atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg
     8221 taatgggtca ggaaaagcta tacatcgaaa aagagctcag ttggttatcg ttcaatgaac
     8281 gcgtgcttca ggaagcggcg gacaaatcta acccgctgat tgaaaggatg cgtttcctgg
     8341 ggatctattc caataacctt gatgagttct ataaagtccg cttcgctgaa ctgaagcgac
     8401 gcatcattat tagcgaagaa caaggctcca acgctcattc ccgccattta ctgggcaaaa
     8461 ttcagtcccg ggtgctgaaa gccgatcagg aattcgacgg cctctacaac gagctattgc
     8521 tggagatggc gcgcaaccag atcttcctga ttaatgaacg ccagctctcc gtcaatcaac
     8581 aaaactggct gcgtcattat tttaagcagt atctgcgtca gcacattacg ccgattttaa
     8641 tcaatcctga cactgactta gtgcagttcc tgaaagatga ttacacctat ctggcggtgg
     8701 aaattatccg tagcgatagc atccgttacg cgctgctgga gatcccatca gataaagtgc
     8761 cgcgctttgt gaatttaccg ccagaagcgc cgcgtcgacg caagccgatg attcttctgg
     8821 ataacattct gcgttactgc cttgatgata ttttcaaagg cttctttgat tatgacgcgc
     8881 tgaatgccta ttcaatgaag atgacccgcg atgccgaata cgatttagtg catgagatgg
     8941 aagccagcct gatggagttg atgtcttcca gtctcaagca gcgtttaact gctgagccgg
     9001 tgcgttttgt ttatcagcgc gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac
     9061 tgactatttc ccgctacgac tccatcgtcc ccggcggtcg ttatcataat tttaaagact
     9121 ttattaattt ccccaatgtc ggcaaagcca atctggtgaa caaaccactg ccgcgtttac
     9181 gccatatttg gtttgataaa gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg
     9241 atgtgttgct ctattatcct tatcacacct ttgagcatgt gctggaactg ctgcgtcagg
     9301 cttcgttcga cccaagcgta ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt
     9361 cacgcatcat cgactcgatg atccacgccg cacataacgg taagaaagtc actgtggtgg
     9421 ttgagttaca ggcgcgtttc gacgaagaag ccaacattca ctgggcgaag cgcctgaccg
     9481 aagcaggcgt gcacgttatc ttctctgcgc cggggctgaa aattcacgcc aaactgttcc
     9541 tgatttcacg taaagaaaac ggtgaagtgg tccgttacgc acacatcggg accgggaact
     9601 ttaacgaaaa aaccgcgcgt ctttatactg actattcgtt gctgaccgca gatgcgcgta
     9661 tcaccaacga agtacggcgg gtatttaact ttattgaaaa cccataccgc ccggtgacat
     9721 ttgattattt aatggtgtca ccgcaaaact ctcgccgtct gttatatgaa atggtagacc
     9781 gcgaaatcgc caacgcgcag caagggctgc ccagtggtat caccctgaag ctaaataacc
     9841 ttgtcgataa aggcctggtt gatcgtctgt atgcggcctc cagctccggc gtaccggtta
     9901 atctgctggt tcgcggaatg tgttcgctga tccccaatct ggaaggcatt agcgacaaca
     9961 ttcgtgccat cagtattgtt gaccgttacc ttgaacatga ccgggtttat atttttgaaa
    10021 atggcggcga taaaaaggtc tacctttctt ccgccgactg gatgacgcgc aatattgatt
    10081 atcgtattga agtggcgaca ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca
    10141 tcatcgacat attgttcagc gatacggtca aagcacgtta tatcgataaa gaactcagta
    10201 atcgctacgt tccccgcggc aatcgccgca aagtacgggc gcagttggcg atttacgact
    10261 acatcaaatc actcgaacaa cctgaataac cctatgccaa tacacgataa atcccctcgt
    10321 ccgcaggagt ttgctgcggt cgatcttggt tcaaacagtt ttcacatggt catagcccgt
    10381 gtggtagatg gtgccatgca gattattggc cgcctgaaac agcgggtgca tctggcggac
    10441 ggcctggggc cagataatat gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg
    10501 tcgctgtttg ccgaacggct acaagggttt tctcctgcca gcgtctgtat agtcggcacc
    10561 catacgctgc gtcaggcgct gaacgccact gactttctga aacgcgcgga aaaggtcatt
    10621 ccctacccga ttgaaattat ttccggtaat gaagaagccc gtctgatttt tatgggcgtg
    10681 gaacataccc aaccggaaaa aggtcgcaaa ctggttattg atattggcgg cggatctacg
    10741 gaactggtga ttggtgaaaa tttcgaacct attctcgttg aaagccgccg gatgggttgt
    10801 gtcagctttg cccagcttta ttttcctggc ggggtcatca ataaagagaa ttttcagcgc
    10861 gctcgcatgg cggcagcaca aaaactggaa actttaacct ggcaattccg tattcagggc
    10921 tggaacgttg caatgggcgc ttccggtacc ataaaagccg cccatgaagt gttaatggaa
    10981 atgggcgaga aagacgggat aattaccccg gaacgtctgg aaaaactggt aaaagaaatt
    11041 ttacgtcacc gtaatttcgc atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca
    11101 gtcttcgttc ctggactggc gattttatgt ggtgtgtttg atgctttagc catccgtgaa
    11161 ctgcgccttt ctgacggggc gttacgcgaa ggcgtactgt atgaaatgga aggacgtttc
    11221 cgtcatcagg atgtgcgtag tcgcaccgcc agcagcctcg ccaaccagta tcacatagac
    11281 agcgaacagg cccgacgagt gctggatacc actatgcaaa tgtacgaaca gtggcgggaa
    11341 cagcaaccga agctggcgca tccgcaactg gaggcgctac tgcgatgggc cgccatgctg
    11401 catgaggtcg ggttgaatat caaccacagc ggtttgcatc gccactccgc ttatattctg
    11461 caaaacagtg acttgccggg ttttaatcag gaacagcagc tgatgatggc gacactggtg
    11521 cgctatcacc gtaaagcgat taagctcgac gatctgccgc gctttacctt gtttaagaag
    11581 aaacagttcc tgccactgat acagctattg cgccttggcg tattactaaa caatcaacgt
    11641 caggcaacca ccacaccgcc aacattgaca ctgattactg atgacagtca ctggacactg
    11701 cgtttcccgc atgactggtt tagtcagaat gcgctggtac tgcttgatct ggaaaaggag
    11761 caagaatact gggaaggcgt ggctggctgg cggttgaaaa ttgaagaaga aagtacccca
    11821 gaaatcgccg cttaatgctt tgcgggccga cgagaaatgt cggcccgcat tattcaggca
    11881 ctttcgcgaa tgggttcgat ttcattcagc gtatcaatta acggttgcgg cttaccaata
    11941 agataaccct gcatataatc gatccccaaa gagagcaccg cctcgcggat ctcttcgttt
    12001 tcaacgtact ctgccactac ccgcattttc ttcattcgcg ccagatggca aatcgacgcc
    12061 acaatctgat aatccagact atttgacaca atattgcgga taaagctgcc gtcaatttta
    12121 agcagatcgg cattcacatt tttaagccgt gcatagctgg cgtagccggt gccgaaatca
    12181 tcaatcgcaa tctggcagcc taattcctga agatgctgca aggtgatttg cgcctgctta
    12241 acattggtca gagcattact ttcggtgact tcaaaaataa gttgccacgc ttcaatctga
    12301 tatttagcca gcaactgact gacttcaaca ggaaaacgag cctgacatac cgaggttgga
    12361 gacagattaa tagcaaaacg gtgagcgggc atcttcgctc tgttttcagc cataaattgc
    12421 agcgtatgct caatgaccca catgtcgata ctcgacgata aaccaaattc gtgcgcgacc
    12481 ggcaagaagc taccggggcc gatcagttca tcattctcac ctttcatgcg cagaagaatt
    12541 tcatggtaaa catcaccacg cataccggta atcggctggg ccatcaggaa aaaatggttg
    12601 tgttccagcg cctgctgtag tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc
    12661 aaatacattg ccccgcaacg ctgcatattt tccggggcgt tggtcacgat ggaaagttcg
    12721 gcgaccgtat ttagctctcc cagcagcagg tagatatgat tcactggcga gcgcacatag
    12781 cagtaactga cgccaatctg cggttgcatc ggcatcccat cccaaaagaa acgaaattgc
    12841 t
//
LOCUS       DAJTYH010000101        12666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTYH010000101 DAJTYH010000000
VERSION     DAJTYH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..414)
                     /locus_tag="OO609_003105"
     CDS             complement(<1..414)
                     /locus_tag="OO609_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCR6634429.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEE"
     gene            complement(674..1294)
                     /gene="sodA"
                     /locus_tag="OO609_003106"
     CDS             complement(674..1294)
                     /gene="sodA"
                     /locus_tag="OO609_003106"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6634430.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1579..2613
                     /gene="rhaT"
                     /locus_tag="OO609_003107"
                     /pseudo
     CDS             1579..2613
                     /gene="rhaT"
                     /locus_tag="OO609_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(2610..3476)
                     /gene="rhaR"
                     /locus_tag="OO609_003108"
     CDS             complement(2610..3476)
                     /gene="rhaR"
                     /locus_tag="OO609_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6634431.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(3532..4368)
                     /gene="rhaS"
                     /locus_tag="OO609_003109"
     CDS             complement(3532..4368)
                     /gene="rhaS"
                     /locus_tag="OO609_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6634432.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4656..6125
                     /gene="rhaB"
                     /locus_tag="OO609_003110"
     CDS             4656..6125
                     /gene="rhaB"
                     /locus_tag="OO609_003110"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6634433.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6122..7381
                     /gene="rhaA"
                     /locus_tag="OO609_003111"
     CDS             6122..7381
                     /gene="rhaA"
                     /locus_tag="OO609_003111"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6634434.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            7623..8447
                     /gene="rhaD"
                     /locus_tag="OO609_003112"
                     /pseudo
     CDS             7623..8447
                     /gene="rhaD"
                     /locus_tag="OO609_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            8457..8771
                     /gene="rhaM"
                     /locus_tag="OO609_003113"
     CDS             8457..8771
                     /gene="rhaM"
                     /locus_tag="OO609_003113"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6634435.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9072..9518
                     /locus_tag="OO609_003114"
     CDS             9072..9518
                     /locus_tag="OO609_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6634436.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            9529..10980
                     /locus_tag="OO609_003115"
     CDS             9529..10980
                     /locus_tag="OO609_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6634437.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            10970..12040
                     /locus_tag="OO609_003116"
     CDS             10970..12040
                     /locus_tag="OO609_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6634438.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12040..>12666
                     /locus_tag="OO609_003117"
     CDS             12040..>12666
                     /locus_tag="OO609_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6634439.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQY"
BASE COUNT         2991 a         3292 c         3355 g         3028 t
ORIGIN      
        1 ttcttctttc gtaccgtact gctgcatgat ggaagcgtaa agtccgccgt tggtcatatc
       61 catcgccgcc accagcgcca gcgttgaaag tccggcaaag aatccaactt caacaccatg
      121 ttccggaata atgcgcgagg caattgccgc aaccacccac gcgacggcaa ttttagttac
      181 caccagcgta ccggatttac gcagtaccgt tcccgtcgcg cttaatttta ttgacgcccc
      241 catgcaaaaa aaccacaccg ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa
      301 tgatccaaaa tatttccccg cccccggcga gaaggtgtgg cacagtgcgc caaggaatag
      361 cgggacgagc atcatccccc ccgggatttt ctcaatcgag cgttttatct gcatttccat
      421 cacctgtcgg catattttta agggctgatg cgtaattcct caatggagca tcagtgttga
      481 tggaaacgaa gatagacatt catttaatca ataaaagtga tcaaaatcac atcaattgaa
      541 acgctgtttt atttttatag gaccattgtt tttattttgg aattgatcac aaaaaaacca
      601 ccgccgttgg cgatggttca ttttttaagc tgatatgcgg cctatacgcc tcattgcagc
      661 aggcggcaaa tgattatttt ttcgccgcaa aacgtgccgc tgcttcgtcc cagttcacca
      721 cgttccagaa ctctttgatg tagtccggac ggcggttctg gaatttcagg tagtaagcat
      781 gttcccacac atccaggccc agaatcggga agccggaagc gccagaaata gcttcaccca
      841 tcagcggaga atcctggtta gcagtagaaa ccaccgccag tttatcgcct ttcagcacca
      901 gccatgccca gccggaacca aagcgggaag ctgccgcttt ttcaaattct gctttgaagt
      961 tatcaacgga gccgaagtca cgttcgatag ccgctttcag gtcaccctgc agggtggtgc
     1021 cttttttcag acctttccag aacaggctgt ggttagcgtg accgccagcg ttgttgcgca
     1081 gtacggtttt cttgtctgct ggcagctggt ccagtttggt gatcagctct tcaaccggca
     1141 ggttggcaaa ttctggcagg ctttccagcg ccgcgttggc gttgtttacg taggtctgat
     1201 ggtgtttggt gtggtggatt tccatggtct gcttatcgaa gtgcggttcc agggcatcgt
     1261 aagcatacgg cagggatggc agggtatagc tcatattcat ctccagtatt gtcgggcggc
     1321 cgattgttaa tgccgcgtaa gcagttggtt cattatagtt aattaaatga tattgaaaat
     1381 gattatcaat gccgtacttt tcgtaagggt atggttttgc aggaaaatgc ccgagatgtg
     1441 aagcaaatca cccacttaat gccgtgattg ccagtaaatc gacaacggcg gcaacaggcg
     1501 aaaggttaat cgacagcacg atttttacac tcatctcgtc ggagatgtga agcgacgaaa
     1561 aatgatgagg ataagaagat gagtaacgcg attacgatgg ggatattttg gcatttgatc
     1621 ggcgcggcca gtgcagcctg tttttacgct ccgttcaaaa aagtaaaaaa atggtcatgg
     1681 gaaaccatgt ggtcagtcgg tgggattgtt tcgtggatta ttctgccgtg ggccatcagc
     1741 gccctgttac taccggattt ctgggcgtat tacagctcgt ttagtctctc tacgctgctg
     1801 cctgtttttc tgttcggcgc tatgtggggg atcggtaata tcaactatgg cctgaccatg
     1861 cgttatctcg gcatgtcgat gggaattggc atcgccattg gcattacgtt gattgtcggt
     1921 acgctgatga cgccaattat caacggcaat ttcgatgtgc tgattaacac cgaaggcgga
     1981 cgcatgacgt tactcggcgt tctggtggcg ctgattggcg tagggattgt aactcgagcc
     2041 gggcagttga aagagcgcaa gatgggcatt aaagccgaag agttcaatct gaaaaaaggg
     2101 ctggtgctgg cggtgatgtg cggcattttc tctgccggga tgtcctttgc gatgaacgcc
     2161 gcaaaaccga tgcatgaagc cgctgccgca cttggcgtcg atccactgta tgttgctctg
     2221 ccaagctatg ttgtcatcat gggcggcggc gcgatcatta acctcggttt ctgttttatt
     2281 cgtctggcaa aagtgaagga tttgtcgcta aaagccgact tctcgctggc aaaaccgctg
     2341 atcattcaca atgtgttact ctcggcactg ggcgggttga tgtggtatct gcaattcttt
     2401 ttctatgcct agggccacgc ccgcattccg gcgcagtatg actacatcag ttggatgctg
     2461 catatgagtt tctatgtatt gtgcggcggt atcgtcgggc tggtgctgaa agagtggaac
     2521 aatacaggac gccgtccggt aacggtgttg agcctcggtt gtgtggtgat tattgtcgcc
     2581 gctaacatcg tcggcatcgg catggcgaat taatctttct gcgaattgag atgacgccac
     2641 tggctgggcg tcatcccggt ttcccgggta aacaccaccg aaaaatagtt actatcttca
     2701 aagccacatt cggtcgaaat atcactgatt aacaggcggc tatgctggag aagatattgc
     2761 gcatgacaca ctctgacctg ccgcagatat tgattgatgg tcattccagt ctgctggcga
     2821 aattgctgac gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt atccagcgca
     2881 aagggacttt tcaggctagc cgccagccgg gtaatcagct tatccagcaa cgtttcgctg
     2941 gatgttggcg gcaacgaatc actggtgtaa cgatggcgat tcagcaacat caccaactgc
     3001 ccgaacagca actcagccat ttcgttagca aacggcacat gctgactact ttcatgctca
     3061 agctgaccga taacctgccg cgcctgcgcc atccccacgc tacctaagcg ccagtgtggt
     3121 tgccctgcgc tggcgctaaa tcccggaatc gctccctgcc agtcaagatt cagcttcagc
     3181 cgctccgggc aataaataat attctgcaaa accagatcgt taacggaagc gtaggagtgt
     3241 ttatcgtcag catgaatata aaagagatcg ccacgggtaa tgcgataagg acgatcgttg
     3301 agtacatgca gaccattacc gcgccagaca atcaccagct cacaaaaatc atgtgtatgt
     3361 tcggcaaaga catcttgcgg ataacggtca gccacagcga ctgcctgctg gtcgctggca
     3421 aaaaaatcat ctttgagaag ttttaactga tgcgccaccg tggctacctc ggccagagaa
     3481 cgaagttgat tattcgcaat atggcgtaca aatacgttga gaagattcgc gttattgcag
     3541 aaagccatcc cgtccctggc gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag
     3601 cgtcgaaaag tggttactgt cgctgaatcc acagcgatag gcgatgtcag tgacgctggc
     3661 ctcgctgtgg cgtagtagat gtcgggcttt catcagtcgc aggcggttca ggtatcgctg
     3721 aggcgtcagt cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg aaagagaaaa
     3781 ttgatccgcc acggcatccc aattcacctc atcggcaaaa tggtcctcca gccaggccag
     3841 aagcaagttg agacgtgatg cgctgttttc caggttctcc tgcaaactgc ttttacgcag
     3901 caagagcagt aattgcataa acaagatctc gcgactggcg gtcgagggta aatcattttc
     3961 cccttcctgc tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata cgctgtggtt
     4021 aacgcgccag tgagacggat actgcccatc cagctcttgt ggcagcaact gattcagccc
     4081 ggcgagaaac tgaaatcgat ccggcgagcg atacagcaca ttggtcagac acagattatc
     4141 ggtatgttca tacagatgcc gatcatgatc gcgtacgaaa cagaccgtgc caccggtgat
     4201 ggtatagggc tgcccattaa acacatgaat acccgtgcca tgttcgacaa tcacaatttc
     4261 atgaaaatca tgatgatgtt caggaaaatc cgcctgcggg agccggggtt ctatcgccac
     4321 ggacgcgtta caagacggaa aaaaatccac actatgtaat acggtcatac tggcctcctg
     4381 atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac
     4441 ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg
     4501 gtgagcatca catcaccaca attcagcaaa ttgtgaacat catcacgttc atctttccct
     4561 ggttgccaat ggcccatttt cctgtcagta acgagaaggt cgcgaattca ggcgcttttt
     4621 agactggtcg taatgaaatt cagcaggatc acattatgac ctttcgcaat tgtgtcgccg
     4681 tcgatctcgg cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt gaatgccgca
     4741 gcctgacgct gcgcgaaatc catcgtttta acaatgggct gcatagccaa aacggttatg
     4801 tcacctggga tgtggatagc ctggaaagtg ccattcgcct tggattaaac aaggtgtgcg
     4861 aggaagggat tcgtatcgat agcattggga ttgatacctg gggcgtggac tttgtgctgc
     4921 tcgaccaaca gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc cgcaccaatg
     4981 gcctgatgcc gcaggcacaa cagcaactcg gcaaacgcga tatttatcag cgtagcggca
     5041 tccagtttct acccttcaat acgctttatc agttgcgtgc gctgacggag caacaacctg
     5101 aacttattcc acacattgct cacgctctgc tgatgccgga ttacttcagt tatcgcctga
     5161 ccggcaagat gaactgggaa tataccaatg ccaccaccac acaactggtc aatatcaata
     5221 gcgacgactg ggacgagtcg ctactggcgt ggagcggggc caacaaagcc tggtttggtc
     5281 gcccgacgca tccgggtaat gtcattgggc actggatttg cccgcagggt aatgagattc
     5341 cggtggtcgc cgttgccagc catgataccg ccagcgcggt tatcgcctcg ccgttaaacg
     5401 gttcacgcgc cgcttatctc tcttctggca cctggtcatt gatgggcttc gaaagccaga
     5461 cgccatttac caatgacacg gcgctggcag ccaacatcac caatgaaggc ggggcggaag
     5521 gtcgctatcg ggtgctgaaa aatattatgg gcttatggct gcttcagcga gtgcttcagg
     5581 aacggcaaat caacgatctc ccggcgctta tcgccgcgac acaggcactt ccggcctgcc
     5641 gcttcatcat caatcccaat gacgatcgct ttattaatcc tgacgagatg tgcagcgaaa
     5701 ttcaggctgc gtgtcgggaa acggcgcaac cgatcccggg aagtgatgct gaactggcgc
     5761 gctgtatttt cgacagtctg gcgctgctgt atgccgatgt gttgcatgag ctggcgcagc
     5821 tgcgcggtga agatttctcg caactgcata ttgtcggcgg cggctgccag aacacgctgc
     5881 tcaaccagct atgcgccgat gcctgcggta ttcgggtgat cgccgggcct gttgaagcct
     5941 caacgctcgg caatatcggc atcgagttaa tgacgctgga tgaactcaac aatgtggatg
     6001 atttccgtca ggtcgtcagc accaccgcga atctgaccac ctttacccct aatcctgaca
     6061 gtgaaattgc ccactatgtg gcgcagattc actctacacg acagacaaag gagctttgcg
     6121 catgaccact caactggaac aggcctggga gctggcgaaa cagcgtttcg cggcggtggg
     6181 gattgatgtc gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa tgcactgctg
     6241 gcagggcgat gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg gggggattca
     6301 ggccacaggc aattatccgg gcaaagcgcg taatgccagt gagctacgtg ccgatctgga
     6361 acaggctatg cggctgattc cggggccgaa acggcttaat ttacatgcca tctatctgga
     6421 atcagatacg ccagtctcgc gcgaccagat caaaccagag cacttcaaaa actgggttga
     6481 atgggcgaaa gccaatcagc tcggtctgga ttttaacccc tcctgctttt cgcatccgct
     6541 aagcgccgat ggctttacgc tctcgcatcc cgacgacagc attcgccagt tctggattga
     6601 tcactgcaaa gccagccgtc gcgtttcggc ctattttggc gagcaactcg gcacaccatc
     6661 ggtgatgaac atctggatcc cggatggtat gaaagatatc accgttgacc gtctcgcccc
     6721 gcgtcagcgt ctgctggcag cactggatga ggtgatcagc gagaagctaa accctgcgca
     6781 ccatatcgac gccgttgaga gcaaattgtt tggcattggc gcagagagct acacggttgg
     6841 ctccaatgag ttttacatgg ggtatgccac cagccgccag actgcgctgt gcctggacgc
     6901 cgggcacttc cacccgactg aagtgatttc cgacaagatt tccgccgcca tgctgtatgt
     6961 accgcagttg ctgctgcacg tcagccgtcc ggttcgctgg gacagcgatc acgtagtgct
     7021 gctggatgat gaaacccagg caattgccag tgagattgtg cgtcacgatc tgtttgaccg
     7081 ggtgcatatc ggccttgact tcttcgatgc ctctatcaac cgcattgccg cgtgggtcat
     7141 tggtacacgc aatatgaaaa aagccctgct gcgtgcgttg ctggaaccta ccgctgagct
     7201 gcgcaagctg gaagcggcgg gcgattacac tgcgcgtctg gcactgctgg aagagcagaa
     7261 atcgttgccg cggcaggcgg tctgggaaat gtattgccaa catcacgata cgccagcagg
     7321 tagcgaatgg ctggagagcg tgcgggctta tgagaaagcg attttgagcc agcgtgggta
     7381 aacactgccg gatgcggcac gagcgcctta tccggcctac gggtcgaatg tccatgcggg
     7441 ctgtcaccct caccccggcc ctctccctga aagggcgagg gagaaaaaca tagcctcgtg
     7501 cggcaacgga gctgaacacc gctccggaca gtcccctcgc ccctacgggg agagggttag
     7561 ggtgagggga acaagccgca ccatgtctaa acaaggcaac ctaatttaca ggaacacaga
     7621 acatgcaaaa cattacccag tcctggtttg tccagggaat gatcaaagcc accaccgatg
     7681 cctagctgaa aggctgggac gagcgcaacg gcggcaacct gacgctgcgc ctggatgacg
     7741 ccgatatcgc gctttatcac gacaacttcc acccacaacc gcgctatatt ccgctcagcc
     7801 aacccatgcc tttactggca aatacgccgt ttattgtcac cggctccggc aaattcttcc
     7861 gtaacgtcca gctcgatcct gcagctaact taggcgtcgt aaaagtcgac agcgatggcg
     7921 cgggctacca tattctctgg gggttaacca acgaagccgt tccaacttct gaacttccgg
     7981 ctcactttct ctcccactgc gagcgcatta aagccaccaa cggtaaagat cgggtgatca
     8041 tgcactgcca cgccaccaac ctgatcgccc tcacctatgt actggaaaac gacaccgcgg
     8101 tctttactcg ccaactgtgg gaaggcagca ccgaatgtct ggtagtattc ccggatggcg
     8161 ttggcattct gccgtggatg gtgcccggca cggacgaaat cggccaggcg accgcacaag
     8221 agatgcaaaa acattcgctg gtgttgtggc ccttccacgg tgtgttcggt tccggaccga
     8281 cgctggatga agcctttggt ttaatcgaca ccgcagaaaa atcagcacaa gtattagtga
     8341 aggtttattc gatgggcggc atgaaacaga ccatcagccg tgaagagttg atagcgctcg
     8401 gcaagcgctt cggcgttaca ccactagcca gtgcgctggc gctgtaagga gcaaacatga
     8461 tccgcaaagc ctttgtcatg caggtaaacc ccgacgccca cgaagagtat cagcgtcggc
     8521 ataatcccat ctggccggaa ctggaagcag tgttgaaatc tcacggtgtg cataactacg
     8581 ccatttacct cgacaaagcg cgtaatctgc tgtttgccat ggtagagatt gaatctgaag
     8641 aacggtggaa tgcggtagcc agtactgatg tttgccaacg ttggtggaaa tatatgaccg
     8701 atgttatgcc cgctaaccca gataacagcc cggtgagtag cgagctgcaa gaagtgtttt
     8761 acctgccgta attgcccaat gtgccggaga tgcctccggc acaatatttt aaatttcgat
     8821 ttgaaaaatt aaacatatta catctcccgg taagttatat ttccctgata cattgtgagt
     8881 aaatcacaaa aataatgaat aacccattaa tgattcatgt ggtttattta aataaccatt
     8941 atgtgcatta ctccgcaaat ctgacctttc actctgtctc aaattgtcat tgtttaccga
     9001 catatcgcct ctcatcactt aatcgctcca ccattacaat tgattaaaat atattttatg
     9061 gagtccgatt aatggcagct cttactgcaa gctgtattga cctgaatatt cagggcaatg
     9121 gcgcttattc cgttctgaag cagttggcga caatagcgtt acaaaacggt tttatcaccg
     9181 actcacacca gttcctgcaa accctgctgc tgcgcgaaaa aatgcactct actggatttg
     9241 gttccggtgt cgcagtgccg cacggtaaaa gcgcctgcgt taaacaaccg ttcgtattat
     9301 tcgcccgcaa agcgcaggct attgactgga aagccagcga tggcgaagac gtcaattgct
     9361 ggatctgcct cggcgtgccg caaagcggcg aagaggatca ggtcaaaatc atcggcacac
     9421 tgtgtcgcaa aattattcac caggaattta ttcatcaact gcaacagggc gataccgacc
     9481 aggtgctggc cttgttaaat caaaccctca gctcataagg aagtggcgat ggagtcatcc
     9541 ttacgtattg tcgcgatcac caactgcccc gccgggatcg ctcacaccta catggtggcg
     9601 gaagccctgg aacagaaagc ccgttctctc ggccatacca taaaagtgga aactcaaggg
     9661 tccagtggcg ttgaaaaccg cttatccagc gaagagattg ccgctgccga ttacgtcatt
     9721 cttgctaccg ggcgtggcct gagcggtgat gatcgcgcgc ggtttgccgg gaagaaagtt
     9781 tatgagattg ccatctccct ggcgttgaaa aatatcgacc agattttcag cgaattaccg
     9841 acaaactcgc agctttttgc cgcagatagc ggcgtgaagc ttggcaaaca ggaagtgcaa
     9901 agcggcagcg tgatgagcca cctgatggcg ggcgtctctg cggcgctgcc gtttgtcatc
     9961 ggcggtggta tcctggtggc gcttgccaac atgctggtgc agttcggttt gccgtatacc
    10021 gatatgtcga aaggcgcgcc gtcgtttacc tgggtagttg aatccatcgg ttaccttggc
    10081 ttcaccttta tgatccccat catgggcgct tatattgcct cgtcgattgc cgataaaccg
    10141 gctttcgctc cggcgtttct ggtttgctat ctggcgaacg acaaagcgct gctcggcacc
    10201 cagtcgggtg cgggattcct cggcgcggtg gtgctggggc tggcgattgg ctatttcgtc
    10261 ttctggttcc gcaaagtgcg ccttggcaaa gcattacaac cgctgctcgg ttcaatgctg
    10321 atcccgttcg ttactctgct ggttttcggc gtgctcactt actacgttat cgggccggtg
    10381 atgtccgacc tcatgggcgg gctgctccac ttcctgaata ccattcctcc atcaatgaag
    10441 tttgcggcag cgtttctggt cggtgcgatg ctggcgttcg atatgggcgg ccccatcaat
    10501 aaaaccgcct ggttcttctg cttctcactg ctggaaaaac acatttatga ctggtacgcc
    10561 atcgtcggcg ttgtcgcgtt gatgccgccc gttgcggcgg gtcttgcgac gttcattgcg
    10621 cccaaactgt ttacccgaca ggaaaaagag gctgccagta gtgccattgt ggtcggtgcc
    10681 acagtcgcaa ccgaaccggc tataccttac gctctggcag ctccgttgcc gatgattacc
    10741 gccaatacgc tggcgggcgg cattaccggc gtgctggtga tcgctttcgg aatcaaacgt
    10801 ctggcacctg gtttgggtat ctttgacccg ctgatcggcc tgatgtcgcc ggtaggttcg
    10861 ttttatctgg tgctggcgat tggcctcgcg ctgaacattt cattcattat cgtcctgaaa
    10921 ggattgtggt tacgccgcaa agcgaaagcc gcgcaacagg agcttgtcca tgaacattga
    10981 gttactgcaa cagttgtgcg aagccagcgc cgttagcggc gatgaacagg aagttcgcga
    11041 catcctgata aacacgctgg aaccttgcgt taatgaaatc acctttgatg gtctgggcag
    11101 ctttgttgcc cgtaagggaa ataaaggtcc aaaagttgcc gttgtcgggc atatggatga
    11161 agtcggcttt atggtcaccc acatcgacga gagcggtttt ctgcgtttca ccaccattgg
    11221 tggctggtgg aatcagtcga tgctcaacca tcgggtaacc atacgcacac acaagggagt
    11281 gaaaatccct ggtgtgattg gtttcgtcgc gcctcatgcg ttaacggaaa agcaaaggca
    11341 acagccgctg tcatttgatg agatgttcat tgatattggc gcaaacagtc gcgatgaggt
    11401 ggaaaaacgc ggtgtggcga tgggcgattt tattagcccg gaagccaatt ttgcctgctg
    11461 gggcgaagat aaagtggtcg gcaaggcgct ggataaccgc atcggctgcg caatgatggc
    11521 tgaactattg cagacggtga ataatcccga aattacgctg tatggcgtcg gcagcgtgga
    11581 agaagaagtt gggctacgcg gggcacaaac ctcggcggaa cacattaaac cggatgtagt
    11641 gatcgtgctg gataccgctg tcgcaggcga tgttccgggc attgataaca ttaaataccc
    11701 gctgaaactg ggccaggggc cggggctgat gctgtttgac aagcgctact tccccaacca
    11761 gaaactggta gcagcgttaa aaaactgtgc cgcacataac gatttaccgc tgcaattttc
    11821 caccatgaaa accggagcga cggatggcgg gcgctacaac gtgatgggcg gcgggcgtcc
    11881 ggttgttgcg ctgtgcctgc caacccgcta tctgcacgct aacagcggca tgatttcaaa
    11941 agccgattac gatgctctgc tcacgttgat acggggtttt ctgacgacct taaccgcgga
    12001 gaaagtcaac gcgtttagcc agttccgtca ggtggattaa tgctaaacga acgccagtta
    12061 aagattgtcg atctgctgga gcaacagccg cgcacgcctg gtgagctggc gcaacagact
    12121 ggcgtttcag gcaggaccat cctgcgtgat attgactatc tcaactttac ccttaacggc
    12181 aaagcccgca ttttcgccag tggcagtgcg ggctatcagc tggaaatctt cgagcgccgc
    12241 agcttttttc agttgctgca aaagcacgat aacgacgatc ggctgctggc gctgttatta
    12301 ctgaatactt tcactccccg tgcgcaactc gcctcggcgc ttaatttgcc agaaacgtgg
    12361 gtagcagagc gtctgcccca gttaaaacag cgttatgaac gcacttgttg cctggccagc
    12421 cgccctggtt tgggccattt cattgatgag acagaagaga aactcgttat cttgctggcg
    12481 aacttgctgc gcaaagatcc gtttttaatt ccgctggcgg gcataacacg agacaacctt
    12541 cagcatttat ccacggcctg cgacaaccaa caccgctggc cgctcataca gggtgattat
    12601 ctctccagcc tgattctggc gatttacgcc ctgcgtaatc aactgaccga tgagtggccg
    12661 caatat
//
LOCUS       DAJTYH010000102        12625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTYH010000102 DAJTYH010000000
VERSION     DAJTYH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            748..831
                     /locus_tag="OO609_003118"
     CDS             748..831
                     /locus_tag="OO609_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6634440.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(885..2237)
                     /gene="gorA"
                     /locus_tag="OO609_003119"
     CDS             complement(885..2237)
                     /gene="gorA"
                     /locus_tag="OO609_003119"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6634441.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2309..3151)
                     /gene="rlmJ"
                     /locus_tag="OO609_003120"
     CDS             complement(2309..3151)
                     /gene="rlmJ"
                     /locus_tag="OO609_003120"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6634442.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3354..5396
                     /gene="prlC"
                     /locus_tag="OO609_003121"
     CDS             3354..5396
                     /gene="prlC"
                     /locus_tag="OO609_003121"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6634443.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5404..6156
                     /gene="rsmJ"
                     /locus_tag="OO609_003122"
     CDS             5404..6156
                     /gene="rsmJ"
                     /locus_tag="OO609_003122"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6634444.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6205..7674)
                     /gene="dtpB"
                     /locus_tag="OO609_003123"
                     /pseudo
     CDS             complement(6205..7674)
                     /gene="dtpB"
                     /locus_tag="OO609_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7992..8426)
                     /gene="uspA"
                     /locus_tag="OO609_003124"
     CDS             complement(7992..8426)
                     /gene="uspA"
                     /locus_tag="OO609_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6634445.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8820..9155
                     /gene="uspB"
                     /locus_tag="OO609_003125"
     CDS             8820..9155
                     /gene="uspB"
                     /locus_tag="OO609_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6634446.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9226..10725)
                     /gene="pitA"
                     /locus_tag="OO609_003126"
     CDS             complement(9226..10725)
                     /gene="pitA"
                     /locus_tag="OO609_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6634447.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10957..12156
                     /locus_tag="OO609_003127"
     CDS             10957..12156
                     /locus_tag="OO609_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6634448.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3103 a         3169 c         3354 g         2999 t
ORIGIN      
        1 gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga taagtcaaaa
       61 acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg aattttgagt
      121 tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact ttgcgtcggt
      181 tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc acatctaacg
      241 gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt cagctggaga
      301 agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat cgggtcgctg
      361 ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc ggttttttta
      421 ctcttcagcc cagagttcgt ggctctggct ggagtgagaa tgccatcatc taacgcatca
      481 atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc taattacact
      541 gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat aaacgctttc
      601 aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg gagtgagatt
      661 gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa gcggatgcat
      721 tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt tcttggggcg
      781 ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg atagcggaaa
      841 cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg catggtcacg
      901 aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt tttggttgcg
      961 cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa gccaatgccg
     1021 tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg gcacggctgg
     1081 cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac tttcacctga
     1141 tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc aatcggcgga
     1201 tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg cttgttatta
     1261 aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag ttccactgca
     1321 cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata tttatcgacg
     1381 acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat gttgtcattg
     1441 gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg accatcttcc
     1501 agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat ggcgttggta
     1561 tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat catcgggtcg
     1621 aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc gaggccgtta
     1681 atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac gcgctctggc
     1741 aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc tggaatatcc
     1801 gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat ggtttcgccg
     1861 ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac atcaacgtta
     1921 tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc ggtacggctg
     1981 gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata atcagggccg
     2041 tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt cggcacgcag
     2101 ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca tttctggccg
     2161 tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat ggcgatgtaa
     2221 tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt gtagcgcgag
     2281 acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt acggtggcgt
     2341 gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta ttcatctgct
     2401 gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg ccacggcgat
     2461 cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg gtcgcttcca
     2521 ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac aatgcgtaag
     2581 tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc tgatagtcgg
     2641 ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc ggcggcagtt
     2701 tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg tctttctgaa
     2761 atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc aggctgtctt
     2821 gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc aactggccgc
     2881 tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc aaatcgtcct
     2941 gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca tgttcgctac
     3001 ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt ttatctttct
     3061 ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg gcgtggttgc
     3121 cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta agtaaaaatt
     3181 ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag aggccgccgc
     3241 cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc tacacacatt
     3301 atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg ctaatgacga
     3361 atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg gaacatgtcg
     3421 ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc gtagtagcgc
     3481 aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg gacgatgttt
     3541 tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc ccggaactgc
     3601 gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg gtagggcaac
     3661 atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac gccacgctga
     3721 acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc tctggcatag
     3781 gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt tctgaactgg
     3841 gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa ctcgttaccg
     3901 acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa gcccaggccg
     3961 aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac ctgccggtaa
     4021 tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac agcacccgcg
     4081 cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg gaagagatcc
     4141 tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc tttaaatccc
     4201 ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc gatctggcaa
     4261 aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc gctaaagccg
     4321 aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa aaacagaaac
     4381 agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa aacaaagcgg
     4441 ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa gagcgtaaag
     4501 atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa aataacgaac
     4561 tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc ggggcgtgga
     4621 tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa ccggtcgcgt
     4681 atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc actcacgacg
     4741 aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg acccgcatcg
     4801 aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa ctgccgagtc
     4861 agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct ggtcactatg
     4921 aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg aagaactacc
     4981 aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc cgccttcatg
     5041 ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa atcaagaaac
     5101 tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc agccatattt
     5161 tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta ctggcggcag
     5221 atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg cagtcgttcc
     5281 tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc aaacgcttcc
     5341 gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag ggctgatcat
     5401 tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt atctgttctg
     5461 gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt gttaacgccg
     5521 gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt
     5581 gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg
     5641 aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac cgcagggctg
     5701 gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct ggagcgtaat
     5761 ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc
     5821 ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac ggcgctaacc
     5881 gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca taagcagaaa
     5941 agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat
     6001 gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt ggtaaagcgc
     6061 ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt gactaaaggg
     6121 catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt agcgataacg
     6181 ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca tgcgtttcag
     6241 ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac caatcttacc
     6301 aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc
     6361 ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat
     6421 cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg ccaggccaag
     6481 ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga taaaccatgg
     6541 cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg tcaaaaagcc
     6601 cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt ctttgccttt
     6661 gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca cccagaacgg
     6721 gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat gatgcacgtt
     6781 gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt aaaacaccac
     6841 cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt tatccagctt
     6901 gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca gcaccagatt
     6961 ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca cgctgccaag
     7021 taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag aaccaatgtc
     7081 tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc ccgccccgca
     7141 caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca gcgataacgc
     7201 tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat caagccgcgg
     7261 atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa acaggccgtt
     7321 accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc
     7381 ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc gtttggtccc
     7441 caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca gtgcagcaaa
     7501 agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa cgaagaaaac
     7561 cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga caaaaaagat
     7621 catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat taatatttat
     7681 taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt
     7741 tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac tattacaaca
     7801 gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa ttttgtattg
     7861 gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta cgctgcatca
     7921 ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg
     7981 tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc aacgtgaacg
     8041 gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca
     8101 caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg gcctaggtcg
     8161 ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag ctcggtcagc
     8221 gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac atcaataagc
     8281 ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtag
     8341 gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga gagatcgact
     8401 gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg
     8461 gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg
     8521 ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga
     8581 tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga atattatatt
     8641 gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg
     8701 ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag
     8761 cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta
     8821 tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc
     8881 gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct
     8941 atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc
     9001 gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc
     9061 gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt ggtctggtgg
     9121 tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa taacgtataa
     9181 aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca ggaactgcaa
     9241 ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa acacccaggc
     9301 catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc
     9361 cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat aactcgccag
     9421 gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt
     9481 cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat
     9541 acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga tggtgctaag
     9601 catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac caggcatctt
     9661 caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg
     9721 atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt tcaggcggtt
     9781 gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg ttgcgccagc
     9841 ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg caggatgctg
     9901 ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt
     9961 ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca acataaccag
    10021 accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga tagcggaaag
    10081 gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc
    10141 cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa
    10201 gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa
    10261 aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc
    10321 attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa
    10381 accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat
    10441 ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat
    10501 ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca ccgccgccat
    10561 aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc
    10621 tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa
    10681 taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat
    10741 tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata
    10801 tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt atcttatata
    10861 attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt
    10921 acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga tgccattatt
    10981 ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt aggacgccgg
    11041 gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc tggcggtggg
    11101 cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca gaatccgcat
    11161 ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct ggtcaataaa
    11221 cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga ctccgcgcag
    11281 cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt cagattgcgt
    11341 agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct gaacggcatg
    11401 actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc ggggctgggc
    11461 gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct gccgacccgc
    11521 gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca ggtgctggcg
    11581 ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg tgagaactta
    11641 ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag ctactggcaa
    11701 ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac cttcctgaat
    11761 gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt tcatctaccg
    11821 aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc gctaaaacag
    11881 ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg cgtacaaccc
    11941 aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa
    12001 ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggtgaagtg
    12061 atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcatgg
    12121 gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc cgtcagccgt
    12181 ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt ctggtgttaa
    12241 ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat agtgtattta
    12301 ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa ggaaattatt
    12361 tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc ctctggattg
    12421 aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt atcaggtggt
    12481 gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa tatggctatt
    12541 cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc ttgccatgag
    12601 agtcgtgata ttattattca tcatt
//
LOCUS       DAJTYH010000103        12500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTYH010000103 DAJTYH010000000
VERSION     DAJTYH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(40..1491)
                     /gene="uxaB"
                     /locus_tag="OO609_003128"
     CDS             complement(40..1491)
                     /gene="uxaB"
                     /locus_tag="OO609_003128"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6634449.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1718..3136)
                     /locus_tag="OO609_003129"
     CDS             complement(1718..3136)
                     /locus_tag="OO609_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6634450.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3290..3634)
                     /locus_tag="OO609_003130"
     CDS             complement(3290..3634)
                     /locus_tag="OO609_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6634451.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3634..4560)
                     /gene="glsB"
                     /locus_tag="OO609_003131"
     CDS             complement(3634..4560)
                     /gene="glsB"
                     /locus_tag="OO609_003131"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6634452.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVVIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4624..5559)
                     /locus_tag="OO609_003132"
     CDS             complement(4624..5559)
                     /locus_tag="OO609_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6634453.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5639..6754
                     /gene="yneK"
                     /locus_tag="OO609_003133"
     CDS             5639..6754
                     /gene="yneK"
                     /locus_tag="OO609_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6634454.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6904..8094
                     /gene="ydeA"
                     /locus_tag="OO609_003134"
     CDS             6904..8094
                     /gene="ydeA"
                     /locus_tag="OO609_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6634455.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8119..8784)
                     /gene="marC"
                     /locus_tag="OO609_003135"
     CDS             complement(8119..8784)
                     /gene="marC"
                     /locus_tag="OO609_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6634456.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            8992..9426
                     /gene="marR"
                     /locus_tag="OO609_003136"
     CDS             8992..9426
                     /gene="marR"
                     /locus_tag="OO609_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6634457.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9446..9829
                     /gene="marA"
                     /locus_tag="OO609_003137"
     CDS             9446..9829
                     /gene="marA"
                     /locus_tag="OO609_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6634458.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9861..10079
                     /gene="marB"
                     /locus_tag="OO609_003138"
     CDS             9861..10079
                     /gene="marB"
                     /locus_tag="OO609_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6634459.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10110..11009)
                     /gene="eamA"
                     /locus_tag="OO609_003139"
     CDS             complement(10110..11009)
                     /gene="eamA"
                     /locus_tag="OO609_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6634460.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11273..12391
                     /gene="ydeE"
                     /locus_tag="OO609_003140"
     CDS             11273..12391
                     /gene="ydeE"
                     /locus_tag="OO609_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6634461.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
BASE COUNT         3282 a         3224 c         2857 g         3137 t
ORIGIN      
        1 gaaacccggt cgaaaccggg ttcagaagta acggtgttat tagcacaacg gacgtacagc
       61 ttcgcgcatc cctttttcaa gaatcgcatc caggtcatta gcaacctgct cgaccagacc
      121 aggcacttgc gtcaggtcct gctcccagtg gtctttttct gccaacacaa tcgctaccag
      181 ttcctgggta ccgataacac ggtcacgatg ctggctccag agttgctgat aacgttccag
      241 ccagtgtgca tcgtcctgta ccggatatgt ttctccgtta cgctcaccac gatagaacgc
      301 aatcaatgcc gctaatgcga aagtaaggcg cgccggaagt gtgccatttg ccttctgccc
      361 tgccagcagc tgcggcagga tgcgggtgcg gaacttggtc ataccgttga gcgcgataga
      421 cagcagctga tgcttaatgt acgggttacg gaaacgcccg gtgactgcac tggcgaaaga
      481 ttccagttca tcacgaggca aatccagtac cggaataatt tcttcgtaga tggctttttc
      541 aacgaatgcg caaatttcag catcgttcat cgcttcacct acggtatcca gccctgcctg
      601 gaacgccacc ggcaccagcg cggtgtgcgc accgttgagg attgccactt tgcgttcttt
      661 atacggttta atatcgtcaa caatcagcac gttgagcgga tatttgtcca ggcgcagctc
      721 agtcgctaag gatttcggcc cctgaatcac aaacaggtaa aagtgttcag cggtgtcgag
      781 gaaaccatcg tgataaccca gttcttcttc cagtttagcc acttcatcgc gcggataacc
      841 ggtaacgata cggtccacca gcgtagaaca gaagctgtta gcctgatcca gccattgaat
      901 aaatgcttct ggcagtgccc actcttgcgc atagcgcagc accagttcac gcaacgcgtc
      961 gccattgtag tcaatcaact cacacggaat gatgatccaa cctttatcca gcgcaccgtt
     1021 gaaatggcta aagcgttcga acagcagacg ggtcagtttt gccggatagc ttaccgctgg
     1081 cgcgtcatcg aatttatcgc ccgcatggta gctgataccc gcttcggtgg tgttagagaa
     1141 aacaaagcgc atttccgggt tgtgcgccag tttcaggaat tcatcgtatt cactgtagac
     1201 gctgatttca cgattaaccg agcgaatcag acgcgcgtcg ctgaccgctt cccccttctc
     1261 attcaggcca cggataatgg tggtgtacag accatcctgc gtgctcagtg acggcgggaa
     1321 tgaagtttca atcggacgaa caacgaccac gccagaattc agatcggtgt gctcattcag
     1381 gagatcgatt tgccagtcaa caaaggcgcg caggaagtta ccttcaccaa actgaatgat
     1441 acgttctgga tactgtgcac cgggaaaatc gcgacgattt agtgttttca caatgggttc
     1501 ccttctgatt agtcatacaa cccgtttgaa ttggtgcgac aggttagcaa actttaatac
     1561 gccgaacccc tgttttgatc aactcctgat gattaatgag cagttttatg agaaaagtgt
     1621 ggcgcggatc atggtttaat cgaggaaata cgccttttcc tggatcataa agtggtagaa
     1681 cacattgcat tcaaatcgcg cggaatgaat aaagatgtca gacaacttcc tcaccgtaac
     1741 gcatagtgct ggtacggttg cgcccatctt tcttcgagcg atacagacac gtatcggctt
     1801 cgaccatcaa tttattaaaa tcatcggtca gcgttcggta cgatgcgcga ccactaccga
     1861 cgccaatact taccgtgaga taaagcgttt tttgttgcca ggtgaatggt tgcagttcaa
     1921 cgcctttacg gattttttcc gccattagca gaccatcgac aggattcacc gacggcactg
     1981 caacagcaaa ttcttcgccg cccatgcgcg ccaccagccc cttatcaccg acaatcttct
     2041 gaatatgccg ggcaaacacg cttaacactt tatcgccaca ttcatgtcca tagttatcgt
     2101 taatgctttt gaaatagtcg atatcaagca gcatgaccgt cagatgttgt gtctgtttca
     2161 gcgatggact tttcagcgcc tcataaagac cggaccgtga gtaaacctga gtcagaaaat
     2221 caaagtcggc tcgcagcgca acttgcttca ttagcaaatt gatcgctgcc acgctaaaag
     2281 aaaccataat tgggcatatc gccatcgtgg caataccgag acgtgcggag aacatttccg
     2341 gaatggagaa cggcgaaccg accgagatat caatcaccga atttgccacc agtacgattt
     2401 ccaccgcacc ggtgacaaag gtcaacagac atgttacctg cggcgtatag cgcactgcac
     2461 accagattaa tgcaggcagc gggaacgcca gactccccgc tccgccaatg actaccgagg
     2521 caataaccga cacaataaac gcgatggcgg gcatcatctg ttctgcttta aagcgcggca
     2581 gcactccagg aatagccaac gtcagcatac aaggcacgat caacacgccc gttgagaatt
     2641 gctcactgaa ccagtccgca agcaaaggcc agaaatccag actatcaata ctgaccgaac
     2701 caatcgcccc gacaatagcg cataataatg cggcaatcag acagtaatta aatagccgta
     2761 aagcacttac tggctcatac ttatttttcc ccagacgctt atcccgcacg accagtaagg
     2821 cgacggtaac aataaacatc atattggaga aattgataac cagtgaaacc agtccccatt
     2881 cggtggttat ggcatcataa accagcatcg ccacataact gatcgcatag taatgcagtc
     2941 gattaagcca gacatagcgg gcaaacaccc ccgccattac gccattcagc ggccagaaaa
     3001 gagaaagttc gtgaaccagc cgtaactctg caccaataaa atagaacagc gtggttagcg
     3061 caaaaatagc gatgctatta cgtagcagat gaccttcctg gaacaaacgg aaagttgaaa
     3121 tggggtgtac atgcattaat tatctgtcca tacaattgcg ggtggcaatt atctcaacgt
     3181 gataagtaaa ttctcataaa ggatagcatg ttcgctaaca gcggcggcaa ataaaggtac
     3241 cctgggaatg ctattttatc gttttccgca attctcatgg ctgattcatc tatacaacca
     3301 gccagtgata gattaccgcc acgatataac gttgttgttc ttcgcttaac gaaacaccct
     3361 gcggaatgtt atgccactta gccagacaac cgcgacagca ggtcgcggtg gcgtgctgag
     3421 cgataaacac cggatgcccg cgcatggggg tttgcttacc gtcattagcc ggtaatgccg
     3481 gagcaagacg tttagcgaca aaatccgccg catgttgttc gatgactggc gcgcctttct
     3541 ccaggcaata ctgacgctct ttcatgccca gacgaaagcg agagcgaaat tttgaacggg
     3601 ataaacgcgc gaagagtgga tcgagagact gcattaataa accgaacgcc ctaactgttt
     3661 cgtcaattgt tcaaggacgg cgatacccgc aagcgagtta cctgcatcat ccagttccgg
     3721 actccagact gcgatggcca tttcatgcgg aacaatcacc acaataccgc caccaacgcc
     3781 agatttcgcc ggtagtccca cccgccaggc aaactccccc gcgttctggt acataccact
     3841 ggtcgccatc agcgcgttaa tttgccgcgc ctgcattggc gtcaccacag gttcatcaat
     3901 atgaatagct ttcccctgat tagccagaaa gacaaacgtc cgggccagct ctacacagct
     3961 cattttcaga gcgcagtaat gaaagtagtt ttgcagaacg gttgtcacgt catgatggaa
     4021 attgccaaac gacttcatca gccaggcgat agccgcattt cgcgcggaat gttcaaattc
     4081 ggaacgcgct accaccgtat cgtatgaaat atcagacaca ccgcttaagc cgcgcacgac
     4141 ttccagcata cgttgccgtg gtgcgcttag tcgcccttgc aacatatcgc agaccaccag
     4201 cgcaccggta ttaatgaacg gattacgcgg aataccctgt tccatttcca gttgcactaa
     4261 agaattgaac ggtgatccag acggatcttt gccgacgcgt tgccagattt cctcttcgga
     4321 gtaatgacgc atggcgacaa cgagactcag cactttggaa atagactgaa tggaaaaacg
     4381 ttcttgcgcg tctccggcct gaaaaagctg tccgtcaacg gtacagatag caatccccaa
     4441 tcgggaaccg tctactgtag ccagcgccgg aatataatcc gcgactttac cctgaccaat
     4501 gagcggccgc acttgccgca agatgttttc taaaattgca ttatccatgg cgactgccac
     4561 tttctactcc tggaccgcag gtctgaaaag acctgcgagt atatcagagc tgaatatgtc
     4621 gcgtcagatc cggtctttcc acactgtctg gatattacag aattcgtgta agccgaaatg
     4681 ggaaagctca cgaccaaagc cactcttttt cacgccacca aaggccactc gcgcgtcgct
     4741 ggcacaataa ccattgataa acaccccacc gcattccaga cgtgccgcca tctgtctggc
     4801 ctgtgtttcg tcggtggtaa aaatggtcgc tgaaaggccg aactcactat cattagccag
     4861 ttccagcgca tgttctgcat ctttcgcaat ggtgattgca gcaacggggc caaacatttc
     4921 ttcccgaaac gcggtcattt ctggggtaac attcgccaga accgttggcg gatagtagtt
     4981 acctgctcca gccatctttt ccccgcccag taacaaacgc gcaccctgcg ccagggtttt
     5041 ctccacctga tgatgcagct catcacgtaa atcaaaacga gccattggtc cgagagcgtt
     5101 ctcttcatca cggggatcgc ccattttcaa ggctgccgca gctgacacaa aacgttcggt
     5161 aaatgccgaa gcaattccct cttcgataat aaagcgtttt gccgctgcac aaacctgtcc
     5221 ggtattctga taacgtccgg ctaccgccgc tttaaccgcc agttccagat cggcatcgtt
     5281 aagcacaata aatggatccg aaccgcccag ttccagtacg cattttttca gtgccgctcc
     5341 agcacgaaca cttccggtca ccgtgacagc agcaatgcgc gagtctttaa tcatctgact
     5401 gacaccgtcg ttgtcggcat tcagccagcc atatacgcct tgcgggattc ccgcatcttt
     5461 aaacacttgg gcaatgagct gagcacagcc catcacattc ggcgcatgtt taagtaagta
     5521 gccgttacct gcaagaatga tgggaacagc gccacgcatc acctgccttt gatagttctt
     5581 gcagctcaaa tgccctatat agtgatgttc cgatgactta tgactatatg gggcaaatat
     5641 ggttacgcca gtaagcatct gcaattacat atctcttcct gatgattttc ccgctcgtaa
     5701 tattgcacct caagtaaaag aagttttaaa ggattttatt gatgcactca gtaccataat
     5761 atgtgatgaa gagtggcgca cgtctttaaa caccaactca gccacaaaaa agatatttaa
     5821 caaccttgat aacttatcat atattcagag gacttctttt cggggtaacg acacactata
     5881 caatgaaaag gttcagttta aactcactta tcctgtcaag aatggaagac acaaagaaaa
     5941 tattgaattt caggtagtaa taaatttaag ccctatttat ttagataatt ttcgccatga
     6001 tggagaaatt aatatttttt gcgctcccaa cccaaagcct gtcactatgg ggcgcgtatt
     6061 tcagaccggc gtcgagcgtg tgctctttct gtttatgaat gattttattg aacaatttcc
     6121 aatgatcaac cttggcgccc ccatcaaaag agcgcataca ccacatattg aacccctgcc
     6181 tccggatcac cataccgctg cagattactt acgccagttt gatttgcttg tcctgaattt
     6241 tatctctcgc ggtaattttg tcatactccc ccgattatgg aataactctg aggttcacag
     6301 atggttcgtc aataaagatc ctaacttgat caccgccatt ctcgacataa cggacagcga
     6361 attaaaagag gatttattgc aaagcctgat ggattcatta ggttctaaca aacatgtaca
     6421 acccgaagtc tgcatctgct tcttatccct tttagcagaa caagaatctc ctcattttca
     6481 ggacttgttt ttatttttcg ccaatatgtt attgcactat caccaattta tgaatcccaa
     6541 tgaaagtgat ctgaatgacg tgttgatgcc agcatcatta agtgatgata aaattatcaa
     6601 acatatggcg cgcaggaccc tcaaactgtt tgtaaaaaat gaaacgccgc caaaagttac
     6661 tcacgaagac ctggtgaaaa acaggcctcg ctcccctgtc agaccaccta tacccgcaac
     6721 cgccaaaacg ccagacctcc ctgaacgtca ttaaaccgtg atgttaccga ctctctgact
     6781 cggaacagaa tcagcgtcag agaaacggaa aacgcgatcc agatcacaaa tgcattgtat
     6841 tcacatcatt aaccgtttta agatcatttc atcacttttt cgcaactcac ccgataatct
     6901 gttatgacaa caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt tacgctggca
     6961 gtcgccgcct tcatcttcaa caccaccgaa tttgtccctg ttggcctgct ctctgacatt
     7021 gcgcaaagtt ttcacatgca aaccgctcag gtcggcatca tgttgaccat ttacgcatgg
     7081 gtagtagcgc taatgtcatt gccttttatg ttaatgacca gtcaggttga acggcgcaaa
     7141 ttactgatct gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt tttatcgtgg
     7201 agctttaccg ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc gattttctgg
     7261 tcgattacgg cgtctctggc gatccgtatg gctccggccg ggaagcgggc acaggccttg
     7321 agtttaattg ccaccggtac agcactggcg atggtcttag gtttaccgct cgggcgcatt
     7381 gtggggcagt atttcggctg gcgaatgacc ttcttcgcga ttggtattgg ggcgcttgtt
     7441 acccttttgt gcctgattaa gttacttccc ttactgccta gtgagcattc cggttcattg
     7501 aaaagcctcc cgctattgtt ccgccgcccg gcattgatga gcatttattt gttaactgtg
     7561 gtggttgtca ccgcccatta cacggcatac agctatatcg agccttttgt gcaaaacatt
     7621 gcgggattca gcgccaactt tgccacggca ttactgttat tactcggtgg tgcgggcatt
     7681 attggcagcg tgattttcgg taaactgggt aatcaatatg cgtctgcgtt ggtaagtacg
     7741 gcgattgcgc tgttgctggt gtgtctggca ctgctgctac ctgcggcgaa cagtgaaata
     7801 cacctcgggg tgctgagtat tttctggggg atcgcgatga tgatcatcgg gcttggtatg
     7861 caggttaaag tgctggcgct ggcaccagat gctaccgacg tcgcgatggc gctattctcc
     7921 ggcatattta atattggaat cggggcgggt gcgttggtag gtaatcaggt gagtttgcac
     7981 tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg cttttgctgc gttaatatgg
     8041 tcaatcatta tatttcgccg ctggccagtg acactcgaag aacagacgca atagttgaaa
     8101 ggcccattcg ggcctttttt aatggtacgt tttaatgatt tccaggatgc cgttaataat
     8161 aaactgcaca cccatacata ccagcaggaa ccccatcagg cgggagatcg cttcaatgcc
     8221 acccttgccc accagccgca taattgcgcc ggagctgcgt aggcttcccc acaaaataac
     8281 cgccaccagg aaaaagatca gcggcggcgc aaccatcagt acccaatcag cgaaggttga
     8341 actctgacgc actgtggacg ccgagctaat aatcatcgct atggttcccg gaccggcggt
     8401 acttggcatt gccagtggta caaaggcaat attggcactg ggttcatctt ccagctcttc
     8461 cgacttgctt ttcgcctccg gtgaatcaat cgctttctgt tgcggaaaaa gcatccgaaa
     8521 accgataaac gcgacgatta agccgcctgc aattcgcaga ccgggaatcg aaatgccaaa
     8581 tgtatccatc accagttgcc cagcgtaata cgccaccatc atgatggcaa atacgtacac
     8641 cgaggccatc aacgactgac gattacgttc ggcactgttc atgttgcctg ccaggccaag
     8701 aaatagcgcg acagttgtta atgggttagc taacggcagc aacaccacca gccccaggcc
     8761 aattgcttta aacaaatcta acattggtgg ttgttatcct gtgtatctgg gttatcagcg
     8821 aaaagtataa ggggtaaaca aggataaagt gtcactcttt agctaacctt gcatcgcatt
     8881 gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt caattcattt gacttatact
     8941 tgcctgggca atattacccc ctacaactaa ttacttgcca aggcaactaa tgtgaaaagt
     9001 accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag
     9061 aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt
     9121 aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg
     9181 ttgtcggtcg acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg
     9241 gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc
     9301 ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta
     9361 acaaaaaacc tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg
     9421 ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
     9481 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
     9541 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
     9601 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
     9661 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
     9721 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
     9781 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aacagctagt tgataacgtg
     9841 acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc
     9901 tcttttccgc gcagagcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca
     9961 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
    10021 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
    10081 ttgttctggc cccgacatct cggggcttat taacttccca cctttaccgc tttacgccac
    10141 cgcaagccaa atacattgat atacagcccg gtcataatga gcaccgcacc taaaaattgc
    10201 agacccgtta agcgttcatc caacaatagt gccgcacttg ccagtccgac tacgggcacc
    10261 agtaacgata acggtgcaac ccgccaggtt tcatagcgtc ccagtaacgt cccccagatc
    10321 ccataaccaa caattgtcgc cacaaacgcc agatacatca gcgacaagat ggtggtcata
    10381 tcaatagtaa ccagactgtg aatcatggat gcggaaccat cgagaatcag cgagacaaca
    10441 aagaagggaa tgattgggat taaagcgctc cagattacca gcgacatcac cgccggacgc
    10501 gttgagtgcg acatgatctt tttattgaag atgttgccac acgcccaact aaatgctgca
    10561 gccagggtca acataaagcc gagcatcgcc acatgctgac cgttcagact atcttcgatt
    10621 aacaccagta cgccaaaaat cgctaaggcg atccccgcca attgtttgcc atgcagtcgc
    10681 tccccgaaag taaacgcgcc aagcacgata gtaaaaaacg cctgtgcctg taacaccagc
    10741 gaagccagtc cagcaggcat accaaagtta atggcgcaga acagaaaggc aaactgcgca
    10801 aaactgatgg ttaatccata ccccaagagc aaattcagtg gtactttcgg tcgtgcaaca
    10861 aaaaagatgg ccggaaaagc gaccagcata aagcgcaaac cggccagcat cagcggtggc
    10921 atgttatgaa gccccacttt gatgaccaca aaatttagcc cccatacgac cactaccagt
    10981 agcgccaaca ccccatcttt tcgcgacatt ctaccgcctc tgaatttcat cttttgtaag
    11041 caatcaactt agctgaattt acttttcttt aacagttgat tcgttagtcg ccggttacga
    11101 cggcattaat gcgcaaataa gtcgctatac ttcggatttt tgccatgcgt ccgatggtta
    11161 ataacaacga cgaggcaaga agggcgctgg tagagcgtcg tagggataag ttcatttgtc
    11221 tgtccggcag tgcgtttcgt tatgttttat tttagagatg taaagaaata gcatggctac
    11281 gctgccattt atgaccattt acttgagtcg ccagtacagc ctgagtgtcg atctaatcgg
    11341 ttatgcgatg acaattgcgc tcactattgg cgtcgttttt agcctcggtt ttggtatcct
    11401 ggcggataag ttcgacaaga aacgctatat gttactggca attaccgcct tcgccagcgg
    11461 ttttattgcc attcctttag tgaataacgt gacgctggtt gtgctctttt ttgccctcat
    11521 taactgcgcc tattctgttt ttgctaccgt gctgaaagcc tggtttgccg acaatctttc
    11581 gtccaccagc aaaacgaaaa tcttctcaat caactacacc atgctaaaca ttggctggac
    11641 catcggtccg ccgctcggca cgctgttggt gatgcagagc atcaatctgc ccttttggct
    11701 ggcagctatc tgttccgcgt ttcccatgct tttcattcaa atatgggtaa agcgcagcga
    11761 gaaaatcatc gccacggaaa caggcagtgt ctggtcgccg aaagttttat tacaagataa
    11821 agcactgttg tggtttacct gctctggttt tctggcttct tttgtaagcg gcgcatttgc
    11881 ttcatgcatt tcacaatatg tgatggtgat tgctgatggg gattttgccg aaaaggtggt
    11941 cgcggttgtt cttccggtga atgctgccat ggtggttacg ttgcaatatt ccgtgggccg
    12001 ccgacttaac ccagctaaca tccgcgcgct gatgacagca ggcaccctct gtttcgtcat
    12061 cggactggtc ggttttattt tttccggcaa caacctgcta ttgtggggta tgtcagctgc
    12121 ggtatttact gtcggtgaaa tcatttatgc gccgggcgag tatatgttga ttgaccatat
    12181 tgcgccgcca ggaatgaaag ccagctattt ttccgcccag tctttaggct ggcttggtgc
    12241 cgcgattaac ccattagtga gtggcgtagt gctaaccagc ctgccgcctt cctcgctgtt
    12301 tgtcatctta gcgttggtga tcattgctgc gtgggtgctg atgttaaaag ggattcgtgc
    12361 aagaccgtgg gggcagcccg cgctttgttg atttaagtcg aacacaataa aaatttaatt
    12421 cagccttcgt ttaggttatc tctgctaata tctttctcat tgagatgaaa attaaggtaa
    12481 gcgaggaaac acaccacacc
//
LOCUS       DAJTYH010000104        12469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTYH010000104 DAJTYH010000000
VERSION     DAJTYH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1731
                     /gene="ldtD"
                     /locus_tag="OO609_003141"
     CDS             <1..1731
                     /gene="ldtD"
                     /locus_tag="OO609_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6634462.1"
                     /translation="SPVAVSEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLP
                     AGYKPVYLNQLQLLYAARDMQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTD
                     PGVNGMARDVVLSDAMMGYLHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALD
                     KGQLPTFVAGLAPQHPQYAAMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALRE
                     ILQRTGMLDGGPKITLPGDDTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRA
                     AYDNELVEAVKRFQAWQGLGADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTEL
                     STGIMVNIPAYSLLYYQNGNQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTL
                     ARKDILPKVRNDPGYLESHGYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGP
                     RNSLGRYKFNMPSSEAIYLHDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGW
                     NDKRISDALKQGDTRYVNIRQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQ
                     IVSKAEQLIR"
     gene            1912..2460
                     /locus_tag="OO609_003142"
     CDS             1912..2460
                     /locus_tag="OO609_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6634463.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2487..3134
                     /gene="gloC"
                     /locus_tag="OO609_003143"
     CDS             2487..3134
                     /gene="gloC"
                     /locus_tag="OO609_003143"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6634464.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3357..4547)
                     /gene="aspC"
                     /locus_tag="OO609_003144"
     CDS             complement(3357..4547)
                     /gene="aspC"
                     /locus_tag="OO609_003144"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6634465.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4732..5820)
                     /gene="ompF"
                     /locus_tag="OO609_003145"
     CDS             complement(4732..5820)
                     /gene="ompF"
                     /locus_tag="OO609_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6634466.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6421..7821)
                     /gene="asnS"
                     /locus_tag="OO609_003146"
     CDS             complement(6421..7821)
                     /gene="asnS"
                     /locus_tag="OO609_003146"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6634467.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(7990..9192)
                     /gene="pncB"
                     /locus_tag="OO609_003147"
     CDS             complement(7990..9192)
                     /gene="pncB"
                     /locus_tag="OO609_003147"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6634468.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9458..12073
                     /gene="pepN"
                     /locus_tag="OO609_003148"
     CDS             9458..12073
                     /gene="pepN"
                     /locus_tag="OO609_003148"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6634469.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12280..12438)
                     /locus_tag="OO609_003149"
     CDS             complement(12280..12438)
                     /locus_tag="OO609_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6634470.1"
                     /translation="MLIEEGKISLDLTVDIPRPRRLGSVRLAELEAEVLQRVMRRGHS
                     EQLIRRHG"
BASE COUNT         3166 a         3133 c         3161 g         3009 t
ORIGIN      
        1 agcccggtgg ctgtcagtga acagggcgag gcactgccgc aggcgcaagc cacggcaata
       61 atggcgggga tccagccatt gcctgaaggt gcggcagaaa aagcccgcac gcaaatcgaa
      121 tctcaattac ccgcaggtta caagccggtt tatcttaacc agcttcaact gttgtatgcc
      181 gcacgcgata tgcaacccat gtgggaaaac cgtgatgctg ttaaagcctt ccagcaacag
      241 ctggcagagg tggcgattgc tggtttccag ccgcagttta ataaatgggt agagttactg
      301 accgatcctg gtgttaacgg gatggcacgt gacgtggtgc tctctgatgc gatgatgggc
      361 tatctccatt tcattgcaaa tattccgatc aaaggcactc gctggctata tagcagtaaa
      421 ccttatgcgc tttcaacgcc gccgctttcg gtgattaacc aatggcagct agcgctggat
      481 aaaggtcaat tgcctacgtt tgttgcagga ctggcaccgc agcatccgca atatgcggcg
      541 atgcatgaat cgttactggt cttactcagt gacaccaaac cgtggcccca actgaccggc
      601 aaagcaacgt tgcgcccagg gcagtggagt aacgacgtac cggcgttgcg cgaaatattg
      661 caacgcacag gcatgttgga cggggggccg aaaattactc tacctggcga tgacacgcca
      721 actgacgcgg tagtcagccc atccgctgtt actgttgaaa cagcagaaac taagccgatg
      781 gacaagcaaa cgacgtctcg tagtaaacct gcgcctgccg ttcgtgccgc ctacgataat
      841 gaactggtgg aagccgttaa acgttttcag gcatggcaag gattgggagc agatggtgct
      901 attggcccgg caacgcgtga ctggttaaac gtaacgcccg cccagcgtgc tggcgtgttg
      961 gcactcaaca tccagcgatt gcgcttgctg ccaacagagc tttctaccgg gatcatggtt
     1021 aacattccag cctattcgct gctctactat cagaacggca atcaggtgct ggattcgcga
     1081 gtcattgtcg gtcgccccga tcgcaaaacg ccgatgatga gcagtgcact taacaacgta
     1141 gtggtaaacc cgccgtggaa cgtaccacca actctggcac gcaaagatat tctgccgaaa
     1201 gtgcgcaacg atccgggata tctcgaaagc catggctata cggtgatgcg cggctggaac
     1261 agcagagaag cgattgaccc atgggaggtt gactggtcta caatcacggc ctcgaattta
     1321 ccgtttcgct tccagcaggc tccaggccca cggaactcgc tggggcgcta taaattcaat
     1381 atgccgagtt cagaggccat ttatttgcat gacacgccga accacaatct gttcaagcgt
     1441 gatacacgcg cattgagctc aggctgtgta cgagtgaata aagcttccga tctggcgaat
     1501 atgctgttgc aggatgcagg atggaatgac aaacgtattt ctgatgcgct gaagcagggt
     1561 gatacacgtt acgtcaatat tcggcagtcg attccggtga atctctacta cctgacggcc
     1621 tttgttggtg cagatgatcg tacccagtat cgtacagata tttacaatta tgatctgcct
     1681 gcgcgatcca gctcgcaaat cgtatcgaaa gcggaacaat taatcaggta aatgaagtag
     1741 ttcggggaaa tcaattgtcg taataattag catgattggg ggcgattctc tgcagccccc
     1801 gtcactgctg gggttgagtc atcttgacgt ctgctttacg ggcggttaag gtgcctcttg
     1861 tgcgccagaa gtgcatataa acgataacat tgagctgtag acttgattat catggacaaa
     1921 ttcgacgcta atcgccgcaa attgctggcg cttggtggcg ttgcactcgg tgccgccatc
     1981 ctgccgaccc ctgcgtttgc aacactctct accccacgcc cgcgcatttt gacactcaat
     2041 aatcttcata ccggagagtc aatcaaagcg gagtttttcg atggcagagg ctatattcag
     2101 gaagaattgg caaaacttaa ccattttttc cgcgattacc gcgcgaacaa aataaagtcc
     2161 atcgacccag gattattcga ccagttgtat cgcctgcaag ggttgttagg cacgcgcaaa
     2221 ccggtgcaac tcatttccgg ttatcgttcc attgatacca acaatgaact acgcgcccgc
     2281 agccgtggag tagcgaagaa aagctatcac actaaaggcc aggcgatgga tttccatatt
     2341 gaaggtatcg cgttaagcaa tattcgcaaa gccgcgttat ctatgcgcgc aggtggtgta
     2401 ggatattacc cacgtagtaa ctttgtgcat attgataccg ggccagcacg gcactggtag
     2461 taatcgctta acgaaacagg ggcagtatga actatcgtat tattccggtc accgcattct
     2521 cccagaactg ttcattgatc tggtgtgaac aaacccgcct ggccgcactg gtcgatcctg
     2581 gcggcgatgc ggaaaaaatc aaacaggaag ttgatgacag cggcctgaca ctgatgcaga
     2641 tcctgctgac gcatggtcat ctggaccacg ttggcgcagc ggcggaactg gcgcaacatt
     2701 acggcgtgcc ggtttttggc ccggaaaaag aagatgagtt ctggctgcaa ggcttgcctg
     2761 cgcaaagtcg tatgtttggt ctggaagagt gccagccgct gacgccagat cgttggctga
     2821 acgaaggcga taccatcagc atagggaatg tgactttaca ggtgttacat tgccctgggc
     2881 atacgccggg tcatgtcgtg ttttttgatg atcgggcaaa gctgctgatt tctggcgatg
     2941 ttattttcaa aggcggagta gggcgcagtg acttcccgcg tggcgatcat aatcaactga
     3001 tttcttcaat caaagataaa ttgctgccac tgggggatga cgtgacattt attccgggtc
     3061 acggaccatt atctacactt ggttatgagc gcctgcacaa tcccttcctg caagacgaaa
     3121 tgcccgtctg gtaaaggcgc ataaaaaagt ccacttttaa tgcgggcctg gatttctggc
     3181 aaagtgcgct ttgtttatgc cggatgcggc acgagcgcct tatccggcct acaaaatcgt
     3241 gcaaattcaa aatattgcag gggacgcgta ggcctgataa gcgtagcgca tcaggcaatg
     3301 ttgcgtttgt catcagtctc agcccgcttt tcagcgggct tcattgtttt taacgcttac
     3361 agcactgcca caatcgattc gcacagcgga gccatgttat ctggtgtcat cccggccaca
     3421 tttacgcgac cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt
     3481 tcttttgtca gaccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg
     3541 cggtttgcac ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc
     3601 tggcgcatat cagtcagctc ttgttcccaa atcgcacgta atgcatcgct gctcaggatg
     3661 gtggcaacaa cagaagcgcc gtgtgctgga gggttagagt agttagcgcg aatcgccgct
     3721 ttcatttggc tgaatgcgcg atcaacgatt tcactgtcgg cagcaaccag agtacaagcg
     3781 ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctct
     3841 ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaaa
     3901 ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caacggagag ttgtgccagt
     3961 gtctgccatt gttccagcgt agggtcgata ccggttgggt tatggcagca gccatggaac
     4021 agcactacgt cgccagcctg agcttcgttc agactgttaa tcagtgcatc gaagtcaagg
     4081 gtgtgatttt ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag
     4141 acgctcttat ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt
     4201 tttgccagga agtcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg
     4261 cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag
     4321 cgaccaaatt caaggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc
     4381 agatactgtt cagccttttt cacgctggtc agtaccgggg ttttgcccgt ctcatcttta
     4441 tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc
     4501 aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat
     4561 tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta
     4621 ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa
     4681 aaaacaggac caacgtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg
     4741 gtaaacgata cccacagcaa cggtgtcgtc tgaacctacg cccagtttgt tgtcagaatc
     4801 gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaagt agtaggttgc
     4861 gcccacttca aagtagttca ccagatcaac atcaccgata ccttctacgt ctttcgcttt
     4921 agatttggtg taagcgatgg acggacgcag accgaaatcg aactggtatt gcgcaactaa
     4981 cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt gtaaatttat tagtgatcgg
     5041 cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcgtactt
     5101 cagaccagta gcccactgtt cagctttttt accgttgcca agaggttgag cttcttgcag
     5161 gttggtacgg tcagctgcac cataagcacc aacgatacca aagccttcgt attcgtagct
     5221 gatagaaccg ccaacaccgt cgccgttaga acggcgtgca gtgtcacgct cgtttttacc
     5281 caggtactga acagcgaagt tcaggccatc aaccagacca aagaagttgg agttacgata
     5341 ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg ctgtatgcag tatcaccacc
     5401 aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc
     5461 gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt
     5521 accagtttga gcgtcagcgc cttcagagtt gttaccctgg aagttatatt cccactgacc
     5581 ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt
     5641 catgtcgcca ttgccaccgt aactgttttc accgttaccc ttggaaaaat aatgcagacc
     5701 aacagtttta ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt
     5761 tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat
     5821 tatttattac cctcatggtt tttttatgac acctgccact gccgtcaata agttctgtca
     5881 ataaaaattt acggaactat tgatgagagt ttggtgtctt tatgtgtctg caggcatctt
     5941 tccattcaaa ctaacgtttc gctaccgtga aagtgctaca aagataaaga tttagtttca
     6001 aaaagaaaaa atatgtaacc aaaagtaaaa attaaggagc tttgtgaaca ccgtcatatt
     6061 tccataggaa cgtgatgata tttacagcaa tttttatcta tttatatgat ttacttatat
     6121 ttaaattaac taaacggaaa ttttgtttct gatggaaact ttatcgacct ggcacaaaat
     6181 cctctttcag acatccagaa tgccaaaaaa tagtatgaaa attgtgctat cagctaagaa
     6241 aaaactaatc cgcaataaga gtacgggtta ttttttgtgc gtaaatgttg caatgtaata
     6301 ttccgtaaca ggatgatcgt tatggcgggg gtgcaaatag attgcttgac ttaacaaaca
     6361 gataacttga cagaaaggat aaaaaagagc cagcaggcgc tggctctttg aggaagaaaa
     6421 tcagaagctg gcgttgcgcg gagtacgtgg gaacggaatc acatcacgca cgttttgcac
     6481 gccagttacg taagcaatca ggcgttcaaa accaagaccg aaacctgaat gcggaacagt
     6541 accgtagcga cgcagatcgc gataccacca gtaatcttct ttattcaggc ccatttccag
     6601 catacgctcg tccagaacgt ccagacgttc ttcacgctgg gagccaccaa tgatctcacc
     6661 gatgcccgga gccagaacgt ccatagccgc aacggtttta ccgtcttcgt tgaggcgcat
     6721 atagaacgct ttaatatctt tcggatagtt tttaaccact accggtgctt taaagtgttc
     6781 ttccgccaga taacgctcat gctcagaaga gagatcgacg ccccagtaaa ccgggttttc
     6841 aaacttcctg ccgcagtttt cgagaatggt cactgcgtcg gtgtaatcca cctgcgcgaa
     6901 atcggcttca atgaagcgtt ccagacgtga aacggcatct ttatctacgc gttcagcgaa
     6961 gaatttcatg tcgtctgcgc gttcttcgag aaccgctttg aagacatatt tcagcatggc
     7021 ttcagccaga cccgcgatat cgttcaggtt agcaaacgcc acttccggct ccagcatcca
     7081 gaattccgcc aggtgacggc tggtgttgga gttttcagca cggaaagtcg ggccgaaggt
     7141 ataaattttg gataatgcgc aagcgtaggt ttcgccgttc aactggccag atacggtcag
     7201 gaaagactct ttaccaaaga agtctttgtc gaaatccact ttgccctgat cgttacgcgg
     7261 caggttttcc agatccagcg tagaaacgcg gaacatttcg cctgcacctt cggtatcaga
     7321 tgcggtaatc agtggcgttg aaacccagaa gaatccctgc tcgttaaaga agcgatgcag
     7381 cgcctgcgcc agcgtatggc gaacgcgcgc gacggcacca atcaggtttg tgcgcggacg
     7441 caggtgagcg acttcacgca gatactcaat gctgtggcgt tttgccgcca tcgggtaagt
     7501 gtctggatct tcaacccaac cagcaacttc aaccttgctg gcctgaattt caaattgttg
     7561 cccctggccc ggcgatgcca cgactttacc cgtcacaatg accgagcagc cggtggtcag
     7621 acgcaggacg tcttcattgt aattgggcag agaattattg atgacagcct gtacaggatc
     7681 aaagcaggaa ccgtcataaa cggcgaggaa ggagatgcca gcttttgaat ctcggcgggt
     7741 acgtacccat ccgcgtacgg tgacttcgct gtcaacggct acgcggccct ggagtacgtc
     7801 ggctacaggc acaacgctca taatattctc tctgttaata gtcggaaaaa ataaacactt
     7861 gtccacccgt aaatgggggg gatacctatg ttacctggca tctgcaatca gacaagcaga
     7921 attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaagggagcc gattagctcc
     7981 ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc gcagcgcccg
     8041 aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt tcgccaccgg
     8101 tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct gggggatatc
     8161 gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga agtggcggta
     8221 tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt tactctgtgg
     8281 atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa ccgggtcgcc
     8341 agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat cacgcaggaa
     8401 agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat actcttccag
     8461 ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt gctgatgtgc
     8521 ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac gcgccagatc
     8581 gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga tggtttcttg
     8641 tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc gcgacatatc
     8701 aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt cgagggcttg
     8761 cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa ccgccagcaa
     8821 aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat ccagcttgcc
     8881 attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta accagttaag
     8941 atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat cctgcaggcg
     9001 caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac ccagcagatc
     9061 gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat gaaacacggc
     9121 ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa caggagaagc
     9181 gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta caataaacat
     9241 cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga tcacaccata
     9301 tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta gcaataaagg
     9361 cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca ggaatagact
     9421 gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc cacaagccaa
     9481 ataccgtcac gattatcgtg cgccggatta ccagattact gatattgact tgacctttga
     9541 cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc atggtgcatc
     9601 agatgctccg cttcgtctca acggcgaaga cctcaaactg gtttctgttc atattaatga
     9661 tgagccgtgg accgcctgga aagaagaaga agagggcgca ctggtcatca gtaatttgcc
     9721 ggagcgtttt acgcttaaga tcattaatga aataagcctg gcggcgaata cggcgctgga
     9781 agggctttat cagtcaggcg atgcgctttg cacccagtgt gaagccgaag gtttccgcca
     9841 tattacgtat tatctcgacc gcccggacgt gctggcgcgt tttaccacca aaattattgc
     9901 cgataaaatc aaatatccct tcctgctttc caatggtaac cgcgttgcgc aaggcgaact
     9961 cgaaaacgga cgccattggg tacagtggca ggacccgttc ccgaaaccgt gctacctgtt
    10021 tgcgctggtg gcaggcgact ttgatgtact gcgcgatacc tttaccacgc gttctggtcg
    10081 cgaagtagca ctggagctgt acgtcgatcg cggcaacctt gatcgcgcgc cgtgggcgat
    10141 gacctcgctg aaaaactcca tgaaatggga tgaagaacgc tttggcctgg agtatgacct
    10201 cgacatctat atgatcgtcg cggtggattt cttcaatatg ggcgcaatgg agaataaggg
    10261 tctgaatatc tttaactcca aatatgtgct ggcccgcacc gacaccgcca ccgacaaaga
    10321 ttacctcgat attgaacgcg ttatcggcca tgaatatttc cataactgga ccggtaaccg
    10381 agtgacctgc cgcgactggt tccagctcag cctgaaagaa ggtttaaccg tcttccgcga
    10441 tcaggaattc agctctgacc ttggttcccg cgcagttaac cgcatcaata atgtgcgcac
    10501 catgcgcgga ttgcagtttg cggaagacgc cagcccgatg gcgcacccaa tccgcccgga
    10561 catggtcatt gagatgaaca acttctacac cctgaccgtt tacgagaagg gcgcggaagt
    10621 gatccgtatg atccacaccc tgctgggcga agaaaacttc cagaaaggga tgcaactcta
    10681 tttcgagcgt catgatggta gcgcagcgac ctgtgacgac tttgtgcagg cgatggaaga
    10741 tgcgtcgaat gttgatctct cccatttccg ccgttggtac agccagtccg gtacgccggt
    10801 tgtgaccgtc aaagacgact acaatccgga aactgagcag tacaccctga ccatcagcca
    10861 gcgcacgcca gccacgccgg atcaggcaga aaaacagccg ctgcatattc cgtttgccat
    10921 cgaattgtat gacaacgaag gtaaagtgat cccgttgcag aaaggcggtc atccggtgaa
    10981 ttccgtgctg aacgtcaccc aggcggaaca gacctttgtt tttgataatg tctacttcca
    11041 gccggtgcct gcgctgctgt gcgaattctc tgcgccagtg aaactggaat ataagtggag
    11101 cgatcagcaa ctgaccttcc tgatgcgtca tgcgcgtaat ggtttctccc gttgggatgc
    11161 ggcgcaaagt ttgctggcaa cctacatcaa gctgaacgtc gcccgtcatc agcaagggca
    11221 gccgctgtct ctgccggtac atgtggctga cgctttccgc gcggtgctgc tcgatgagaa
    11281 gattgatccg gcgctggcgg cagaaatcct gacgctgcct tctgtcaatg aaatggctga
    11341 actgttcgat atcatagacc cgattgctat tgccgaagta cgcgaagcac tcactcgtac
    11401 tctggcgact gaactggcgg atgagctgct ggctatttac aacgcgaatt accagagcga
    11461 gtaccgtgtt gagcatgaag atattgcaaa acgcactctg cgtaatgcct gcctgcgctt
    11521 ccttgctttt ggtgaaacgc atctggctga cgtgctggtg agcaagcaat accatgaagc
    11581 aaacaatatg accgatgcgc tggcagcgct ttctgcggcg gttgctgcac agctgccttg
    11641 ccgtgacgcg ctgatgcagg agtacgacga taagtggcat caggacggtc tggtgatgga
    11701 taaatggttt atcctgcaag ccaccagccc ggcggcgaat gtgctggaga cggtgcgtgg
    11761 tctgttgcag catcgctcat ttaccatgag caacccgaac cgtattcgtt cgttgattgg
    11821 tgcgtttgcg ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg gttaccagtt
    11881 cctggtggaa atgcttaccg acctcaacag ccgtaacccg caggtagctt cacgtctgat
    11941 tgaaccgctg attcgcctga aacgttatga tgccaaacgt caggagaaaa tgcgcgcggc
    12001 gctggaacag ttgaaagggc tggaaaatct ctctggcgat ctttacgaga agataactaa
    12061 agcactggct tgatactttt gagccaaaat gcctgatgcg acgcttgacg cgtcttatca
    12121 ggcctacaac tccgttgaaa ttgctggttt tgtaggctgg ataaggcgtt cacgccgcat
    12181 ccggcagccg tgcccaactg cctgatgcga tgcttgacgt gtcttatcag gcctacaagt
    12241 ccgttgaaaa tgctggtttt gtgcgccaca tccggcatat taaccatgac gccgaatcag
    12301 ttgttcagaa tgcccacgcc gcattacccg ttgcaatact tccgcttcca gctccgctaa
    12361 tcgcaccgac cctaatcgac gcggacgggg gatatccact gtcaaatcca gactaatttt
    12421 tccctcttca attaacaaca cccggtcagc cattgctacc gcttcgctc
//
LOCUS       DAJTYH010000105        12203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTYH010000105 DAJTYH010000000
VERSION     DAJTYH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..995
                     /locus_tag="OO609_003150"
     CDS             <1..995
                     /locus_tag="OO609_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6634471.1"
                     /translation="ADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGE
                     GLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQI
                     IASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQA
                     QVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQ
                     LKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLY
                     HRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            996..3161
                     /locus_tag="OO609_003151"
     CDS             996..3161
                     /locus_tag="OO609_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6634472.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3175..4086
                     /locus_tag="OO609_003152"
     CDS             3175..4086
                     /locus_tag="OO609_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR6634473.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4086..5381
                     /locus_tag="OO609_003153"
     CDS             4086..5381
                     /locus_tag="OO609_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6634474.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5426..5656
                     /locus_tag="OO609_003154"
     CDS             5426..5656
                     /locus_tag="OO609_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634475.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5634..6134
                     /locus_tag="OO609_003155"
     CDS             5634..6134
                     /locus_tag="OO609_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6634476.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6134..7552
                     /locus_tag="OO609_003156"
     CDS             6134..7552
                     /locus_tag="OO609_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR6634477.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7552..8400
                     /locus_tag="OO609_003157"
     CDS             7552..8400
                     /locus_tag="OO609_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634478.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8400..8855
                     /locus_tag="OO609_003158"
     CDS             8400..8855
                     /locus_tag="OO609_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6634479.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8858..9550
                     /locus_tag="OO609_003159"
     CDS             8858..9550
                     /locus_tag="OO609_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6634480.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9560..10891
                     /locus_tag="OO609_003160"
     CDS             9560..10891
                     /locus_tag="OO609_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6634481.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10892..>12203
                     /locus_tag="OO609_003161"
     CDS             10892..>12203
                     /locus_tag="OO609_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6634482.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQA"
BASE COUNT         3275 a         3035 c         3220 g         2673 t
ORIGIN      
        1 cggctgatgg cgttgaagat ggcatttcaa tctgctactt caagccatac tcgcaaggcc
       61 gtgctcgctg gcagggtgac acaatccacg gcgtgtggtt tgacgaagag ccaccataca
      121 gcatttatgg cgaaggtctt acccgtacca acaaatacgg gcaattctca attctgacgt
      181 ttaccccgct gatggggatg tctgacgttg ttaccaagtt cctgaagaac cccagcaagt
      241 cgcagaaagt ggtcaacatg accatctatg acgctgagca ctataccgac gagcagaaag
      301 agcaaatcat cgcatcatat cccgagcatg agagagaggc gcgtgctcgc ggtattccta
      361 cgatgggtag tggtcgaatc ttccagatac cggaagagac gattaagtgt cagccgttcg
      421 agtgtcctga tcacttctac gtaattggcg ggatggattt cggatgggat cacccacagg
      481 cgcaagttca gctttggtgg gataaggagg cagacacaat ctacgtttca cgcgtgtgga
      541 aggcgaaaga aaaaacagcc gttcaggcgt ggggagccgt taaatcatgg gcgcataaag
      601 tgccaaccgc atggcctcat gatggaaacc agcacgagaa gggcggcggt gagcagctca
      661 aagggcagta tgcagacgct ggatttatga tgttgcagga gcatgcgaca tggcctgatg
      721 gcggtaacgc tgttgagcca ggcatcactg aattgcgcga catgatgctc gatggtcgct
      781 tcaaagtatt caatacctgt gagccattct ttgaggagtt ccgcctctat caccgtgatg
      841 aaaacgggaa aatcgtcaag cttaacgacg acgtgctatc cgccgttcgc tatgcataca
      901 tgatgcgccg cttcgcaaaa atgatgcgcg acatcaaaaa accaaaagag aaaaagatac
      961 cagccccaat caggcccatc gcacggagaa cttaaatggc cgacgaaaac agactcaatt
     1021 ccattctgtg taagtttgac gcagactgga tggcgagcga tgaagccaga accgaggcga
     1081 caaatgacct gtattttagc cgagtgtcgc aatgggatga ctggctatca aactacacta
     1141 ccctgcaata tcgcggacaa ttcgatgttg ttcgcccgga ggtcaggaaa ctggtcgcag
     1201 agatgcgccg gaaccctgtc gacgttctct tccgacccaa agacggcgct aatcctgatg
     1261 cagccgatgt attgatgggg atgtatcgta ctgatatgcg ccataacacg gcaaagattg
     1321 ccgttaacgt tggcgttcgt gagcagatag agtcaggcgt tggtgcatgg cgtctggtca
     1381 cgcagtacga agacaacgat ccaacaagca acaatcaggt aatccgacgc ctgccaatcc
     1441 atgaagcctg ctcacacgtc atatgggacg ccaacagcaa gcagatggat aagagcgacg
     1501 ctaagcactg cacggtgatt aacgccttgt cgcgcaatgg ctggaaagag ttcgcagagg
     1561 attacggtat tgacccggat accctgccat ctttccagaa tcctaacgat acatggctat
     1621 tcccgtgggt gtcgaatgat gtcgtctacg tcgctgagta ttacgaggta gaagaggaga
     1681 aggagaaggt cttcatctac cgcgacccgc tgacaggtga gccggtcagc tattaccagc
     1741 aggatatcaa agacgtcatc gacgacctgg ctaatcgtgg attcattaag gtagcagagc
     1801 gcaaggtgaa gcgtcggcgt gtgtataagt cgatcatcac ctgtacgcag atactgaaag
     1861 accgcgagaa gatagccgga gagcatattc caatcgttcc agtgtatggc gaatggtcat
     1921 tcgctggtga caaggagtgc tacgagggtg tggtaaggct gacgaaagac ggtcaacgcc
     1981 ttcgtaacat gattatgtcg ttcaacgccg atattgttgc tcggtcaccg aagaagaaac
     2041 cgaccttctt ccctgagcaa atcgaaggct acgaatacat gtacggcgga aatgatgact
     2101 atccgtacta tctgcagaac aggactgatg aaaacggtaa cgacctgccg attggtccaa
     2161 tctcctacat ggaaaaccct gaagtgccgc aagccaacgc ttacatgctt gaggctgcca
     2221 ccaacgcagt gaaagaagtg gctagtcttg gcgtggatgc gcaggcggca aatggtcagg
     2281 tcgcttttga taccgtcaat caactgaaca tgcgggcaga ccttgagaca tacgtgtttc
     2341 aggataacct ggctaccgca atgcgacgtg atggcgagat ttatgcctca atggtcaacg
     2401 atatttatga cgttcctcgt catgtaacgc tgacacttga agatggaagc gagaaagacg
     2461 ttcaactcta tgcgcaagtt gtcgattacc agtccggcaa tgtggtcaca ctcaacgaca
     2521 ttcgcggtcg atatgagtgc tatacagacg ttgggccatc cttccagagt atgaaggaac
     2581 agaaccgcgc agagattcag gagttgctca ccaaggttcc gcaaggtact ccagagttcc
     2641 agatgctgat gctgcaatac ttcacgctgc ttgacggtaa aggcgtcgag atgatgcgag
     2701 agtacgcgaa caagcaactg gtgatgatgg ggctgaagaa accagaaaca cctgaagaga
     2761 tggagatggt gcagcaggca caacaacagc cgcagcagcc atcagcagag caaattcagg
     2821 cgcagggtat ccttctgcaa ggtcaggctg aattgctcaa ggcagagaac caacaggcgc
     2881 agattcaggt tgaagctgcc aaggttgaag cccaaaacca actcaacgcc gcgaagattg
     2941 cagaaatatt caacaatatg gacctcgaca agcaggcaga actgcgtgag tacctcaggc
     3001 tcgtaggtca attccagcaa cagcgcagca aagatgctcg tgctaacgct gagctgcttc
     3061 ttaaagatgc agaccagact cattcacaac gcatggattt cgcgaatctt atgcgtcaag
     3121 ttcaaatccc ctccggcgga gtagccgaga cacctcaata agagagagtt aatcatggac
     3181 caaaccaccg acattcaggc ttctgaagaa ttaaccctgc ccggcaatca tgcagcggca
     3241 tctgctgatg gcttagttgt cgataatgcc aacgacagcg caggtcagga agaaggcttt
     3301 gagattgtcc ggaaagacga tgagaaacca aaacaagacc cggcaactaa tgctgaattt
     3361 gcccgtcgcc gcatcgaacg caaacgccag cgtgagcttg agcagcagat ggaagcggtt
     3421 aagcgtggag agttgccgga gcacctgcgg gtgaaccctg agttaccaaa acaaccagac
     3481 cctaacgatt atctttccga agatgcactg gctaagtacg actatgacca gagccgcgca
     3541 ctggctgcct tccagcaggc aaacagtgaa tggcagatca aggctatgga cgcacgaagc
     3601 caggctgtcg ccgagcaggg gcgcaaaact caggagttca cccagcaatc agcgcaatac
     3661 gtcgaggcgg cccgtaagca ctacgaggca gcggaaaagc tcaatatccc tgactatcag
     3721 gagaaagagg atgcattcat gcaactggtg ccgccagcag tcggtgccga catcatgcgc
     3781 ctcttcccgg agaaatccgc cgctctcatg tatcaccttg gtgctaatcc tgagaaaaca
     3841 cgccagttgc tggcgatgga cgggcaatcc gcgctgattg aactcactcg actgtcagaa
     3901 cgtttaactc tcaagcctcg agccaagcct gtttcagaag ccccgttacc tgatgaaccc
     3961 attcagggac atgctgttgc tgcaaatatc tctgcgattg aaaagcagat ggaggcggca
     4021 gcaaacaaag gggatgtaga gacgtaccgc aagctcaagg cgcaactgaa taaaggaatt
     4081 cgataatggc attaaatgaa ggtcaactgg tcacgtatgc tctggatgaa atcatcggaa
     4141 ccgtccagaa cctgacgcca atggcgtcaa aagtgacaaa atacccccct ccggcagaat
     4201 ccatgcagcg ttcaagcaac accgtgtgga tgcctgttga gcaggaagcg ccaacccaga
     4261 ctggctggga tttaactggc aacgcaaccg ggattctgga actctccgtg aagtgcaaca
     4321 tgggcgatcc ggataacgat ttcttcgagc ttcgtgcaga tgacctgcgt gatgagcgtt
     4381 cttaccgtcg ccgcatccag gcatccgcca aaaaactggc gaataacatt gagtcagcga
     4441 ttgccaaaca ggcaactgaa atgggctcgc ttgttgttca cgatacccgc gcaattggtc
     4501 catcgactgg cctgcctggc tgggattttg tgtctgatgc agagcgactg atgttctccc
     4561 gtgagctaaa ccgcgacatg ggcatcagtt acttcctgaa ccctgacgat taccgcaaag
     4621 caggccgcaa cctggtagat ggtgacatct tcgggcgcgt tcctgaagaa gcatatcgaa
     4681 acggtactat tcagcgtcag attgctggct ttgatgaaat tcttcgctca ccgaaacttc
     4741 cggcagttac caagtcaacc gcgactggtg taactgtttc tggtgcgcag aagtttaagc
     4801 cgcaggcata cacccttgat accgatggta acaaagagaa cgtcgacaac cgtgttgcaa
     4861 cggtgaccgt atcctccacc accggattta agcgcggcga caaaatcagc ttcattggtg
     4921 tgaagttcct gtctcagatg gcgaagaatg tgctgactga tgacgcgact ttctcaatca
     4981 cccgtgtgat cgatggtact cacatcgaaa tcacgccgaa gccgattgcg ctggatgacg
     5041 cgtcactgac aaaagaagag aaggcttacg ctaacgtaaa cacctctctt gctgatacca
     5101 ctccggtaaa cgttctgaac gtggcaacaa ccaccgctaa cgtgttctgg gctgatgact
     5161 caatccgcct gctgtctcag ccgatcccgg taacccatga actgtttgct ggcatgaaaa
     5221 cgtcttcctt cagcattcca ggtattggtg ttaacggcat cttcgcaacg cagggtgata
     5281 tcaacactct gtctggtaag tgccgtattg ctgtgtggta ttcagcatgt gctgtacgac
     5341 cagaggcaat tggtgttggt ctgcctaacc agaccgcgtg ataaccagag ggagcttcgg
     5401 ctcccgtttt tatctggaga caagcatgac acacatgatc tttcgtcatg gcgacatgaa
     5461 gaagtggaaa ggcgttggct acgactttga aatcgtgaaa gccgaagagc ttcaggaata
     5521 tctggatgct ggctggtttt cacatcctga tgaccttttg aaggatgttg cagagccaga
     5581 gccagaagaa aaacagcgta aaaagcctgg tcgaaaacct aaggcggcag cagatgaacc
     5641 tgacaacgaa gggtgattta gttcttgcgg cattacgtaa gctcggtgtt gcatcaaatg
     5701 ccacgttaac cgatgtcgaa ccgcagtcta tggaagacgg cgtcaacgac cttgaaatga
     5761 tgatggctga atggcttggc ggtgatgcgt cacctggtat caacgttggc tacatttttg
     5821 ctgatgcaga tgttgctccg gatccgggcg atgagcacgg tttatcaaat aacgctatca
     5881 atgccgtcat tttcaacctt gcctgccgaa ttgctccaga ttatgcgctg gaagcgtctg
     5941 caaaacttat aaccactgcc agatacggga aagagcgact cgtcaaactg tctgcaatgg
     6001 acagggcaaa agcagctaaa tgtaagtccg gttatccaaa ccgtatgcct gttggtagtg
     6061 gtaaccagtt ggcgaagtgg aatggttgga attacttcca ccgaaaggaa ccttgcgata
     6121 acgggagcga ataatgccta ttcagcaact tccgcttatg aaaggtgtcg gcaaagactt
     6181 ccgaaacgcc gactatatcg actatctgcc agtgaatatg ctggctacac ccaaagaaat
     6241 cctgaacagc agcggatatc ttcgctcatt cccgggcatt gccaaacgtt ctgacgtgaa
     6301 cggtgtatct cgcggcgtcg agtacaacat ggcgcagaat gctgtttatc gcgtgtgtgg
     6361 cggcaagctg tacaaaggag aaagtgaagt cggtgatgtt gccggaagtg gtcgtgtatc
     6421 aatggcgcat ggtcggacat cacaggcggt aggcgttaat ggtcaactgg tcgagtatcg
     6481 ctatgatggc acggtcaaaa ccgtctcaaa ctggcctaca gacagtggat tcactcagta
     6541 tgagttaggt tcagttcgcg acattacgcg cttacgtggg cgttatgcgt ggtcaaaaga
     6601 cggaactgat tcatggttta tcactgacct tgaagacgaa tctcatcctg accgttacag
     6661 cgcacaatat cgtgccgagt ctcagcctga cggcatcatc ggtatcggga catggcgaga
     6721 cttcatcgtc tgctttggtt catcgactat tgaatatttc tccctgactg gcgcaaccac
     6781 cgttggtgcc gctttgtatg ttgcacaacc atcgctgatg gtgcagaaag gcattgccgg
     6841 aacttactgc aaaacgccat tcgctgattc gtatgcgttt atcagcaatc cggcaacggg
     6901 tgcgccgtct gtgtatatca tcgactccgg tcaggtgtca ccaattgcca gcgcgagcat
     6961 tgagaaaatc ctccgctcct acactgctga tgaactggct gatggtgtga tggaatcgct
     7021 gcgatttgat gctcatgagt tgctgattat ccaccttccg cgtcacgtcc tcgtgtacga
     7081 cgcatcttca atcgccaatg gcccacaatg gtgtgtgctg aaaacaggtc tgtatgacga
     7141 tgtgtaccgc gctatcgact tcgtttacga aggcaatcag ataacgtgcg gcgataagct
     7201 ggaatctgtt accgggaaac tgcaattcga tatcagcagc cagtacgaca agcaacagga
     7261 acacctgctg tttactccac tcttcaaagc ggataacgcc agagtgttcg accttgaggt
     7321 tgaatcgtca actggcgttg cgcagtacgc cgaccgcctt tttctctctg caaccactga
     7381 ctgcatcaat tacgggcgtg agcagatgat tgagcagaat gaaccgttcg tttacgacaa
     7441 gcgtgttttg tggaagcgag tagggcgcat caggaaaaat gtcggcttca aattgcgcgt
     7501 tatcacgaag tcacctgtca ctctgtctgg cgctcagata aggattgagt aatggctgat
     7561 tcgaatctca atgtgccggt aatcatccaa gctacgcggc tcgatacatc agtccttcca
     7621 cgcaatatct tctcgcagtc gtatctgctt tacgttatcg cacagggcac tgatgttggt
     7681 aacgtggcta acaaagccaa cgaggccgga cagggcgctt atgatgcaca ggtcaggaac
     7741 gatgagcagg atgtgattct cgctgaccat gagcagcgaa tttctgctgc ggaagcaacg
     7801 cttgttaatc atgaggagcg aatcagacag gcagaatcaa ctcttcagga ccatgaaaca
     7861 cgaatagctc agaatgaaag cgatatttcg tcgcttgaca caagagttca gtcgctggag
     7921 tcgcaggttt cagaccatga aacgcgcatc gatgctctgg agtatgcaac cacacgcaag
     7981 aagtcagagg ttgtttactc tggtgtatct gtaaccatcc cgacagcgcc gaccaacctt
     8041 gttagcctgc tgaaaacgct cacaccgtca tccggctcgt tggcaccatt cttcgacacc
     8101 gttaacaaca agatggttgt gttcaacgag aacaaaaccc tgctcttcaa gttgtcgatt
     8161 gtcggcacgt ggcccagcgg aaccgccaac aggtcaatgc agctaacctt ttccggctct
     8221 gttcctgaca cactggtcag cagtcgtaat gcggcgacaa caaccgacaa catcctgtta
     8281 gctacgttct tcagcgtgga taaagacggc tttcttgcca caaatggcag tacgttaacc
     8341 attcagtcga atggtgcggc gtttactgcc acaaccatca agataatcgc ggagcagtga
     8401 tgattacatt caaaccaacg cgaaacatcg acctgataga agcagtcgga aatcaccctg
     8461 acattatcgc cgggagcaac aacggtgatg gatacgacta caaacctgaa tgccgttact
     8521 ttgaggtgaa cgtgcacggt cagtttggcg gcattgttta ctatcaggag attcagccgc
     8581 tgacattaga ttgccacgcc atgtacctgc cagagattcg cggcttcagc aaggaaattg
     8641 ggctggcgtt ctggcgatac attctgacta acaccaccgt tcagtgcgta acatcgttcg
     8701 ctgcacgcaa attccgacac ggtcagatgt actgcgcaat gattggcctt aagcgtgtag
     8761 gaaccatcaa gaaatacttc aaaggcgtgg atgacgtgac tttttacagc gccacacgcg
     8821 aagaactaat cgacttcctg aatcacggga gatagccatg ttatatgcat ttaagctggg
     8881 cagaaaactg cgcggcgagg aaccttattg ccctgaaaaa ggcgggaaag gtggcagttc
     8941 tgataaaagc gcaaagtatg cagcagaagc ccagaagtat gctgcagacc tgcaaaatca
     9001 gcagtggcag acgatcatga aaaaccttgc tccgttcacg cctcttgcgg agcagtatgt
     9061 taaccagctt cagaaccttt ccagtttaga aggtcagggg caggcactta atcagtatta
     9121 caactctaag cagtataaag accttgcagg gcaggcgcgt taccagagtc ttgctgctgc
     9181 ggaggcgacg ggtggacttg gttcgacagc cacaagcaat caactggcta cgatcgcgcc
     9241 gacactcggt cagtcttggt tatcaaatca gatgagcaat tacaacaatc tggcaaacgt
     9301 tgggcttggt gcgctgcaag gtcaggcaaa cgccgggcag acgtacgcca acaacatgag
     9361 cagcattgca cagcaaagcg cagctcttgc cgctgctaat gccaataaac catcaagtct
     9421 tcagactgca attagcggtg gcacgtctgg tgcgattgcc ggtgcaggtc ttgccagcct
     9481 tttgggaaca tcaacgcctt ggggcgctgg tattggtgct ggtatcggat tgcttggctc
     9541 gttgttttaa gggggaatca tggctacttg gcaaggaaca aacggcggat tgttggctgg
     9601 tatcggcggc gtcaactcaa acgctccgag cgtaaatgac atcggcaata cgcttcagct
     9661 tatcaggcag aacaatgata ttgagcgttc aggcgctaac aatgttgggc tgactgcttt
     9721 gcaaggtctt tcaggtattg cgggtgtttt tcagcaggaa aagcaggctc agcggcagaa
     9781 agaatttcag caggcgtacg ctaatgctta tgcgtctggt gatcgcggtg ctttgcgtca
     9841 gttggctact caatatccag accagattga atccgttcgt aaaggcatgg gattcattga
     9901 tgaagagcag cgtaattcta tcggcacctt agcggctggc gcacgccttg cgtcatcgtc
     9961 tccagaagca atgcaatcat ggctgcaaaa caacgccggt gagttagctc gtgttggcgt
    10021 taatcctcat gacgtcgctc agatgtacca acagaacccg cggcagttcg gcgaatttgt
    10081 cgatcacctg gggatgaaca gtctcgggcc cgaaaaatac tttgacctac aggataaaat
    10141 gcagggtcgc caggttacca tgcgcggtca ggatctggat tcgcaaaccg ccgctcggaa
    10201 tcaggcaatc acaatgcgcg gacaagatat ccaggcgaat ttaggtcagc agcgcattaa
    10261 tctggacgca gaaacaaacc gcattaacaa cgaaaataag cgccttgacc ggatgctatc
    10321 agcagaaact aacgacctga agcgccagga aatacagagc cgcatagcag ccaacaacca
    10381 gcagttgcag cagaagcagc aagcgctaaa tgatggctac aaagacggca tcaacaccct
    10441 cacaaccagc atgttcactc tgaacgatat cgttagttct ccttcactta agagcattac
    10501 aggcttacgt ggagtaatcc ccaacgttcc aggctcacag gctgcagaca ctcaggcacg
    10561 acttgatacc tttaaatcac aagcatacct gacagcggtt caggccatgc gaggcatggg
    10621 cgcactttct gatgccgagg gcaaaaagct cgaccaggct gttggttcgc tgcagaactc
    10681 gcagagcgag gagtcctttc gtcgcaacgc tggcgtcatc ctgaacacgc tcaaccagaa
    10741 gcgtaatgag gcggttggta agtacgttca gcaaaacggt atcaagcgag tggaagcgcc
    10801 tcaggcttct atagattacc tgaagcagca ccccgagctg tcaatcgact tcattaatcg
    10861 ctacggatat cttccatctt tggggcagta aatggctaat taccgtgatt tgttagagca
    10921 ggctggcgca cgttacggtg tgccagaagg gttgatgact gcactgggtg ccaaggagtc
    10981 ttcttacaac cctgccgcag taagctccgc cggggctgta ggattgactc aggtcatgcc
    11041 tgggacatgg cgtgatatgg gttataccga tgagcaaatg caaaaccccg aatatcaggc
    11101 tgacgctggc gcgcgctatc tggcaaagat gtaccagcag tttggtaact ggcgtgactc
    11161 tcttcaggct tatcacgacg gtcccggcaa cgttatgaag gcaaagcgtg gtgaatatac
    11221 gccaggacct gaaggccgcg gttacgttga tgatcgcttt gctcaatggg ctggtgaccc
    11281 ggtgacagac tcaacagtcg aacagcgcgc cacctctgca aaggtacatc ctcagcaaga
    11341 ccctaacaac ccgtttgcac aactggaagt acagtcatcc gaacaagtat cggcatcagg
    11401 tgtgcagtca gacccaaata atccatttgc tcagattgag cagcaggcag ccagtcagca
    11461 gccacctcaa cccgtaagtt ctgtcgcacc gaaacctgtt cagcaacaaa cagttaatca
    11521 ggccaataat gcaccaacac gggaagaacc atcattgatg caacaagctg gcgattggct
    11581 cactggtggt caaagtgcag ggcaaattgc agaacaggct ggtcgtggtc tggtaaacat
    11641 accatttgac gtattgcagg gtggcgcaag tctgattaat gcaatcagtc aggggcttgg
    11701 ggggccaaaa gtattggatg atgtttatcg tccagtagac agaccgacag acccctacgc
    11761 gcaagctgga gagtcaatag gcggttatct tgttcctgga gcaggtgtag ctggaaacat
    11821 ggtcattggt tctctcgctg acgcggcgaa tcaacggggt gattttgccg aaaatgccgc
    11881 tattaatgcc ggacttagca ttgctacgca tggcctgata aatggcgtta cccgtggtgt
    11941 tcgtggtgca tcaaatataa ttagtggcaa taaaacatct gcacagagag cgaccactgc
    12001 gccaacagaa acatcaccat tctccggtga tgccgctgca gcaacaaatc ctgcggttca
    12061 tgccgcagag gcaagagtag cacaaggtgt accaatgacg cctgcgacga agaacccaga
    12121 ggaagtcgtt cgcacagtag cagcacaaaa aaggccaaat ctcgcttcat cgcttgatga
    12181 actagatatc aatcctcagg ctg
//
LOCUS       DAJTYH010000106        12105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTYH010000106 DAJTYH010000000
VERSION     DAJTYH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(105..743)
                     /gene="rutR"
                     /locus_tag="OO609_003162"
     CDS             complement(105..743)
                     /gene="rutR"
                     /locus_tag="OO609_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6634483.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1031..2122
                     /gene="rutA"
                     /locus_tag="OO609_003163"
     CDS             1031..2122
                     /gene="rutA"
                     /locus_tag="OO609_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6634484.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2122..2814
                     /gene="rutB"
                     /locus_tag="OO609_003164"
     CDS             2122..2814
                     /gene="rutB"
                     /locus_tag="OO609_003164"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6634485.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2826..3212
                     /gene="rutC"
                     /locus_tag="OO609_003165"
     CDS             2826..3212
                     /gene="rutC"
                     /locus_tag="OO609_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6634486.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3220..4020
                     /gene="rutD"
                     /locus_tag="OO609_003166"
     CDS             3220..4020
                     /gene="rutD"
                     /locus_tag="OO609_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6634487.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4030..4620
                     /locus_tag="OO609_003167"
     CDS             4030..4620
                     /locus_tag="OO609_003167"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6634488.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4631..5125
                     /gene="rutF"
                     /locus_tag="OO609_003168"
     CDS             4631..5125
                     /gene="rutF"
                     /locus_tag="OO609_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6634489.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5146..6474
                     /gene="rutG"
                     /locus_tag="OO609_003169"
     CDS             5146..6474
                     /gene="rutG"
                     /locus_tag="OO609_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6634490.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6556..6663)
                     /locus_tag="OO609_003170"
     CDS             complement(6556..6663)
                     /locus_tag="OO609_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634491.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7036..7632
                     /gene="wrbA"
                     /locus_tag="OO609_003171"
     CDS             7036..7632
                     /gene="wrbA"
                     /locus_tag="OO609_003171"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6634492.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7653..7880
                     /locus_tag="OO609_003172"
     CDS             7653..7880
                     /locus_tag="OO609_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6634493.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7918..9159)
                     /gene="agp"
                     /locus_tag="OO609_003173"
     CDS             complement(7918..9159)
                     /gene="agp"
                     /locus_tag="OO609_003173"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6634494.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9452..10708)
                     /locus_tag="OO609_003174"
     CDS             complement(9452..10708)
                     /locus_tag="OO609_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6634495.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10969..11889
                     /gene="cbpA"
                     /locus_tag="OO609_003175"
     CDS             10969..11889
                     /gene="cbpA"
                     /locus_tag="OO609_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6634496.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11889..>12105
                     /gene="cbpM"
                     /locus_tag="OO609_003176"
     CDS             11889..>12105
                     /gene="cbpM"
                     /locus_tag="OO609_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6634497.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAV"
BASE COUNT         2896 a         3073 c         3055 g         3081 t
ORIGIN      
        1 aacgttcgct gagtgtgaat accgctgccg ctggcggtaa cgccagttta atgacgatcg
       61 gttaaacagg tagccggagg atgttacaac ctcctccgtc atctttaacg tggtcgaatc
      121 ccctcaataa taatccgctg cacgttttca accgtttgat tgaaaaatac ctcatcgcgc
      181 aacgtcgcgc ctgtcaccgc ctccacctga ggggcgaaat cggcgtaatg ttgagtggaa
      241 gcccaaatca taaaaatcaa atgctgcgga tcaatcggcg cgagtttgcc gcttttgacc
      301 caaccggcaa tcagcgccga tttctcatca attaatgcct tcaaatcgcc cgtcagttca
      361 tccattaaca acggcgcgcc tgccagcatc tccatacaga acaggcgcga agcctgcgga
      421 taatcgcgtg agacttccag cttcagacgg atgtactctt ttatcgccgc cagcggggcg
      481 aaatcttcac gaaacgcttt taacggtgcc agccagatat cgagaatctg ccgcagcacg
      541 gcaatataca gcgcctcttt tgacgggaag taatacagca gattggtttt tgaaacaccc
      601 gccaactctg cgatctgctc cagccttgtg ccgtgaaaac cgaattgtga aaaagtgtcc
      661 agtgctgcgc taagaatcgc ttttttcttc gcgcttactg cgcgcgaacg tttacccgtt
      721 gttttcactg cgccttgcgt catgcgctct cccctcttgt agattggtaa caggatagca
      781 aaacccccgc ccggcctcga ccttctgcac tctcatcgcg cacggatgca tgttttatgt
      841 gcaactgttt tgaccgttta gtccactttt taccagatat tttagataac acaatgctaa
      901 cacattaata atattcactt tttaaaaact ggcatccgct ttgcgaacaa gccaatacgc
      961 ggctgaaaag gatatgcagg atgcagcgcc ccgtctgact ttcactttac gagacgaaga
     1021 gaggttgatg atgaaaattg gcgtattcgt acctattggc aacaacggct ggctcatttc
     1081 gacccacgcg ccgcagtaca tgccgacctt tgaactgaat aaagccattg cgcaaaaagc
     1141 ggagcattac catttcgatt tcgccctgtc gatgatcaaa ctgcgtggct ttggcggcaa
     1201 aactgagttc tgggatcaca accttgagtc gttcaccttg atggcgggtc tggcagccgt
     1261 gacctcgcgc attcagattt acgccactgc tgccacctta acgttacctc cggcaatcgt
     1321 cgcccgtatg gccgcaacca tcgactccat ctctggcggg cgttttggcg tcaacctcgt
     1381 gactggctgg caaaagcccg agtatgagca gatgggtatc tggcctggcg atgactattt
     1441 ctcccgtcgt tacgactatc tcaccgaata tgttcaggtg ctgcgcgacc tgtggggcac
     1501 ggggaaaagc gattttaaag gcgatttttt caccatgaat gattgtcgcg tcagtccgca
     1561 accgagtatc cccatgaaag tgatctgcgc cgggcaaagc gacgctggca tggcgttctc
     1621 cgcccagtat gccgatttca acttctgttt cggcaaaggc gtaaatacac ccacggcttt
     1681 cgccccgacc gctgcgcgga tgaaacaggc cacagagcaa accggacgcg acgttggctc
     1741 ttatgtgttg tttatggtga ttgccgatga aaccgacgat gccactcgcg ccaaatggga
     1801 acactacaaa gcgggcgcgg atgaagaggc gttaagctgg ctaaccgaac aaagtcagaa
     1861 agatacccgc tccggtactg acaccaacgt tcgtcagatg gccgatccca cttcggcggt
     1921 aaacatcaat atggggacgt tagtcggttc ttacgccagt gtcgcgcgca tgttagatga
     1981 agtcgcaagc gtgcctggtg cagaaggcgt gctgttgacc ttcgatgatt ttctgtcggg
     2041 aatcgaaacc ttcggcgagc gcattcaacc actgatgcag tgccgcgccc atctctctgc
     2101 gctgactcag gaggtggcat gatgacaacc ttaaccgctc gaccggaagc cattaccttc
     2161 gatccgcagc aaagtgcgca gatcgtggtg gatatgcaaa acgcttatgc cacgccaggc
     2221 ggctacttag atctcgccgg gtttgatgtc tcaaccactc gcccggtcat tgccaacatt
     2281 caaaccgctg tgaccgcagc gcgagcggca gggatgttga tcatctggtt tcaaaatggc
     2341 tgggatgaac agtatgtcga ggctggcggc cccggctcac cgaattttca taaatcgaac
     2401 gccctgaaaa ccatgcgtaa gcagccgcag ctgcagggga aattgctggc gaaaggctcc
     2461 tgggattatc aactggtgga tgaactggtg ccgcagcctg gcgatattgt gctgccgaag
     2521 ccgcgctaca gcggtttctt caatacgccg ctggacagca ttttacgcag ccgtggaata
     2581 cgccatctgg ttttcaccgg tatcgctacc aacgtctgcg tcgaatcgac gctacgcgat
     2641 ggcttttttc tggagcattt cggcgtggtg ctggaagacg caacacacca ggcggggccg
     2701 gaatttgcac agaaagccgc gttgttcaat atcgaaacct tttttggctg ggtcagcgac
     2761 gtcgaaacgt tctgcgacgc gctttctccc acgtcctttg ctcgtatcgc ttaaggagtt
     2821 tagcgatgcc aaaatccgta attattcccg ctggcagcag cgcaccgctg gcccccttcg
     2881 ttcccggcac gctggctgat ggcgtggtgt atgtctccgg tacgctggct tttgatcaac
     2941 ataataacgt gctgtttgcc gatgacccaa aggcgcaaac ccgccacgtt ctggaaacta
     3001 tccgcaaggt gatcgagacg gcgggtggca cgatggcgga tgtgaccttc aacagcatct
     3061 ttattaccga ctggaaaaat tacgccgcga ttaacgaaat ctacgccgag ttttttccgg
     3121 gtgataaacc ggcgcgattc tacattcagt gcggactggt aaaacctgac gcgctggtgg
     3181 aaatcgccac aattgcgcat atcgccaagt gaggccgcga tgaaactttc actctcatct
     3241 cccccttatg ctgatgcacc cgtagtggtg ttgatttcgg gtcttggcgg tagcggcagt
     3301 tactggttac cgcaactggc ggtgctggag caggagtatc aggtagtctg ttacgaccag
     3361 cgcggcaccg gcaataatcc cgacacactg gcagaagatt acagtatcgc ccagatggca
     3421 gcggaactgc atcaggcgct ggtagccgca gggattgagc gttacgcggt ggtcggccat
     3481 gcgctcggtg cgctggtggg gatgcagctg gcgctggatt atcccgcgtc ggtaactatg
     3541 ctggtcagcg ttaacggctg gctacgaata aacgcccata cgcgccgctg ttttcaggtt
     3601 cgcgaacgat tactgtatag cggcggcgcg caagcatggg tggaagcgca gccgttgttc
     3661 ctctatcccg ccgactggat ggcggcccgc gcacctcgcc tggaggcaga agacgcgctg
     3721 gcactggcgc attttcaggg caaaaataat ttactacgtc gacttaacgc cctcaaacgc
     3781 gctgacttta gtcaccatgc ggatcgcatc cgctgcccgg tgcaaatcat ctgcgccagt
     3841 gatgatctgc tggtgccaac agcatgttcc agtgaacttc atgccgccct gcccgatagc
     3901 cagaaaatgg tgatgcccta tggcggacac gcctgcaacg tgaccgatcc cgaaacgttt
     3961 aatgctctgt tactcaacgg gcttgccagc ctgttacatc accgtgaagc cgccctgtaa
     4021 ggaattgcta tgaacgaagc cgttagccca ggtgcgctta gcaccctgtt caccgatgcc
     4081 cgcactcaca acggctggcg ggagacaccc gtcagcgatg agacgttaca ggagctttat
     4141 gccctgatga aatgggggcc gacatcagct aactgttctc cggcacggat cgtgtttatc
     4201 cgcactgcag aaggaaaaga acgtctgcgc ccggcgcttt ccagcggcaa tctgcaaaaa
     4261 accctgaccg cgcccgtcac cgctatcgtc gcctgggaca gtgaatttta tgaacggtta
     4321 ccactactgt ttccccacgg tgatgcccgc agttggttta cctccagccc acaacttgcc
     4381 gaagaaacag cgtttcgcaa cagttccatg caggcggctt atctgatcgt cgcttgccgt
     4441 gcgctgggac tggataccgg cccgatgtcg ggttttgacc gtcaacacgt tgacgacgcc
     4501 ttttttgcgg gcagcacgct gaagagcaat ctgctgatta atatcggcta tggcgatagc
     4561 agcaaacttt ttgcgcgcct gccacgtctg tcctttgaag aagcctgcgg gctgttgtaa
     4621 ggagcaaact atgaacatag ttgatcaaca aacctttcgc gatgcgatgt cctgtatggg
     4681 cgcggcggtc aatatcatca ccacggacgg tccagccggg cgcgccgggt tcaccgccag
     4741 cgccgtctgc agcgtgaccg acacgccgcc cacattactg gtgtgcctga atcgtggggc
     4801 gtccgtctgg ccggtattca atgaaaatcg aacgctgtgt gtaaatacac tgagcgccgg
     4861 gcaggagccg ctttcaaatc tttttggcgg caaaacgccc atggaacacc gctttgccgc
     4921 cgcccgctgg cagacgggtg tgaccggatg cccgcaactg gaagaggctc tggtttcgtt
     4981 tgactgccgt atcagccagg tggtgagcgt cggcacccac gacattctgt tttgcgccat
     5041 cgaagcgatt catcgtcaca ccacacccta cgggctggtg tggttcgatc gcagttatca
     5101 cgcgctgatg cgccctgctt gttaacctcc ttaaggagac agctgatggc aatgttcggt
     5161 tttcctcact ggcagttaaa atcgacctct acagagagcg gcgtggtcgc gccggatgaa
     5221 cgattgccgt ttgctcagac ggcagtaatg ggcgttcaac acgcggtggc gatgtttggc
     5281 gcgacggtgt taatgccgat tctgatgggg ctggatccca atctttccat tttaatgtcg
     5341 gggatcggca cgttactgtt tttctttatt accggcgggc gcgtccccag ttatctcggc
     5401 tccagcgcgg cttttgtcgg cgtggtgatt gccgcgacgg gctttaacgg tcagggcatt
     5461 aacccgaata tcagcatcgc gctgggcggg attatcgcct gtgggctggt ttataccgtc
     5521 attggcctgg tggtgatgaa aattggtaca cgctggattg aacgactaat gccgccggtg
     5581 gtgacgggtg cggtggtgat ggcgattggc ctgaacttag cgcctatcgc agtgaaaagt
     5641 gtatcggctt cggcctttga tagctggatg gcggtaatga cggtgctgtg tattgggctg
     5701 gtggcggtgt ttacccgtgg catgatccag cggttattga ttctcgtagg attaattgtc
     5761 gcctgtctgc tgtatggcgt gatgaccaac gttttagggc tgggaaaagc cgtcgatttt
     5821 actctcgtca gctatgctgc ctggttcggt ctgccgcatt tttccactcc tggttttaat
     5881 ggccaggcga tgatgttgat tgcgcccgtg gcggtgattc tggtggcgga aaacttaggt
     5941 catctcaagg cggtcgccgg gatgaccgga cgcaatatgg acccgtatat ggggcgagca
     6001 ttcgtcggtg atggtctggc aacgatgctt tccggctctg tcggcggtag cggcgtcacc
     6061 acctatgctg aaaatatcgg tgtgatggcc gtgacaaaag tctactcgac gctggtgttt
     6121 gttgcagctg ccgtcatcgc tatgctgttg ggcttttcgc cgaaatttgg cgcgctgatc
     6181 cataccattc cggccgcggt gattggtggt gcatcaattg ttgtgttcgg gctgattgcc
     6241 gtcgcaggcg caaggatatg ggtacaaaac cgtgtcgatt taagccagaa cgctaatttg
     6301 attatggtcg cagtaacctt cgtgctgggc gcgggtgatt ttgctctcac gcttggcggt
     6361 tttacgttgg gagggatcgg tacagcaacc tttggcgcga ttttactcaa tgcgttatta
     6421 agcagaaggt tggttgacgt tccaccgcca gaagttgttc atcaggagcc ataaacagaa
     6481 aaccgccggg ttaccggcgg tgatgacttt ttgacgagct taagaaaaaa atgcattcgt
     6541 cattgattag cccggctagt tgtcgctttt gccgtgactg ctcttgccac cttttttacc
     6601 tgcttctgat gctctttcgc ggtcttctgc aaaattgccg gaaccgcctc gatggtttgc
     6661 cattactgac ctccgtctgg gtatagacat catattgcat agaattaacg cggcgtatgg
     6721 cggctgccat attttcgcta cgtgggacta agcttagtga actgacgcgg acagacccgc
     6781 gtttggtaat atcctgcaac agcttcttag cgagaataga tatcatctcc aataattatc
     6841 cataagccgc tcttatgatt aaaaaaacgc gatgagataa aaatgtgtca ttttgcgaca
     6901 aaattacgtg cttgtgaaag ttgttataaa tcaaataagt ggttgtgaaa tttgcactct
     6961 gaaaaggacg tcttatcttt aaataagtgg tagcgaatcg ctacggaata gagataacac
     7021 gaggagtggt tagaaatggc taaagttctg gtgctttatt attccatgta cggacatatt
     7081 gaaacgatgg cacgcgcagt cgctgagggt gcaagcaaag tggatggcgc agaagttgtc
     7141 gttaagcgtg taccggaaac catgccgccg caattatttg aaaaagcagg cggtaaaacg
     7201 caaactgcac cggttgcaac cccgcaagaa ctggccgatt acgacgccat tatttttggt
     7261 acacctaccc gctttggcaa catgtccggt caaatgcgta ccttcctcga ccagacgggc
     7321 ggcctgtggg cttccggcgc actatacgga aaactggcga gcgtctttag ttccaccggt
     7381 actggcggcg gtcaggaaca aaccatcaca tccacctgga cgacccttgc gcatcacggc
     7441 atggtgattg tccccattgg ctacgcagcg caggaattat ttgacgtttc acaggttcgc
     7501 ggcggtacgc cgtacggcgc aaccaccatc gcaggcggtg acggctcacg ccagccaagc
     7561 caggaagaac tgtctattgc tcgttatcaa ggggaatatg tcgcaggtct ggcagttaaa
     7621 cttaacggct aatcttcaac aggaggatac gcatgccaac tcaagaagcg aaagctcatc
     7681 acgtcggtga atgggcatct ctgcgcaata cgtcgccgga aatagccgag gccattttcg
     7741 aagtcgccgg gtacgacgaa aaaatggcag aaaagatttg ggaagaaggt agtgatgaag
     7801 tgttagtcaa agcctttgct aagaccgata aagattcgct tttttggggc gaacagacca
     7861 tcgaacgtaa aaacgtttaa ccagcgactc ccccgcttct cgcgggggag ttttctgtta
     7921 tttcaccgcc tcattcaaca cgctatcaaa cttatccatc gggcagaaac cgttggcatc
     7981 tatcgggcaa ccgcttaatt ccagcgtcac gcgctgcgca ggtgcctgca gggttaacgc
     8041 atcggcatta cgtaactgtt ccgcactctg gtacacatat tcaattttca tcaaatcgcg
     8101 attggctttg ctgtcatgcc aacgctggaa aacgattttg ccgccaatcg gcgtgcgttc
     8161 gttctggtca tgcaactgat acggtttgaa atccagcgcc gttaacagag aggctatgtt
     8221 ggagtcgtgc ccaactaaca ccgtgatttt cggagcactg gcgcgatcgg tgaccagggc
     8281 tttgtcgata taactgacca gcggtttcgc aacattgcgc gccacttccg gtgaggtaaa
     8341 cagactgtcc tggtaaccgt ttttcagctt cgacaatacc ttccactgct ggtcagattt
     8401 gatttctccc caggccacct gatccatcgg aaaaccttcg tagtattgca aagtaaacgc
     8461 atctaccagc gagttgccga ctttcagcgg cccggaaaca cctggttctt gttgatactt
     8521 ggcgctaaag gtatttttgc catccaccag cgaacactgt tgtttctctt tacaggcagg
     8581 ggaatcttta tagttaacga ttttttccag tagctggtag ctgtcggtaa gctggagttt
     8641 gctgagctct ttctccattg ccgccaccgc ctgttcactg aatgcggcgg aatcatcggt
     8701 gatcatcggg ttaaaggttg ggtccatagt gcccattttt tcctggtgat gcacaggaat
     8761 atcacaccca gggaatgcac cagtaataaa gaactgggcg gtagcgacgg tacgttgcag
     8821 actattggca taggcataaa cggtgtccgg cggcgggcat tcccccgatt tcactatccc
     8881 ctgctctgcc agccattcac gcatgtaatg gcccatatac acttcgagca cgccaccttt
     8941 ggtggtgagt tgcccaccgg ggacgtccca ttcgggccat ttattcggcg tcgactgctc
     9001 cagcacactg ccattgttcg ccagcggcgc acgtaagtta tggcggctca tcatgagcac
     9061 ttgctgtagc tgatagcctt ccggtacggt ttgtgcctga gcatttgaag cgagtaaaac
     9121 tatccctgcc acagttgcgg cgattagcgt tttgttcatt cctggcacct cttttgttat
     9181 caatcattaa agagtgtgac agaaatatga cttttttccg ggaacaggtt cgcaaaatgg
     9241 cttgttgaac gcctgttttt ctatatacat cttcaaaaac aggcaaggtt tgacgcaaac
     9301 tatgcctgat tagcccctgc ctcgcgtacg ccgctgataa ttgcacctgt ggctaaacag
     9361 aatatctgcg gcgttaccaa tacagagaca atatttacca ataaaaaagt gtgagactaa
     9421 taacaatggt ttttagcata tgcctttgcc attaagttgc ataacgattt aaatagtcaa
     9481 gttgcgcatt catttcctta ttttttaact tataagcctg agaaaaaagt tcaggaggga
     9541 ggtaaacatc ataatgattg ccagtgtttg ttaaatatcc atactcaaga tcttcatctg
     9601 ttgaaaagga ttcatttccc aaggagattg atgccacatt actattaaca ataaataatg
     9661 agcaaatgct atctgaaaga acagcaagaa aattcttcac cgctttaatc ctatccgacg
     9721 tgtcggtatc taaagcaagc aaattcttgt tatcattaaa tataaactcg ggtaaatgca
     9781 gtatgcattt tttgtcattc aagcaactat caatgaattg ttttttaaaa aacagaaagc
     9841 tccatgacat aaaactaccc acaccctgaa ttttgttgtc agattgcaga tcaacgccaa
     9901 ataatgtctt tacatcttcc ctgagattat agaatgccgt atcaacaaga gcctcgatcg
     9961 tttgtttatc aacaatttta ataaactctt cgtatacttg cttttgcgtc ttatggctgc
    10021 ccagcgccca tgaatactca tgttctttta aaaccagtat ggctcttaac agacaatatc
    10081 catcgccatc tactggagcg tattttaatc catatgtttt attaatttca ctctgaataa
    10141 aagatatgcc agacaaatca ataccagtat ctaaatcaga tattcccggc atatttatct
    10201 gttctggctg gatactcata ttttcattat ctaaacaata ctcagaaaaa acatcatcac
    10261 acgcattaat gttttcaata tattccataa agccatacca ataataacct ttaaaatctg
    10321 tgatagactc tgaacctctg cccggcaagg acactctaac aatatcaaca tcttgaacat
    10381 attgtatagt atatgtaata cctcctgaaa cccctgaata aatttgtgta tcattagtac
    10441 gcaatgttct cataaataat gatagatcat tatcattctg catttcctca aattcttttt
    10501 ctagtgattc acattccaga catggagtat cctcttcatt tactttacgt ctgagttcat
    10561 tgtacaaccg cataaaacac accagtgtgt tttcaaaaag cgtgattgaa tatgtttgat
    10621 ttttttcgta ctcatttttt atatcgttcc aggcctgttt caaataatta tttgcagtgc
    10681 aactaattcc atgtatatta ctacccatat atagcgtcta taaaatttaa taaataatga
    10741 cgccctagtt aaacttaaag tgcctggttc aactatcaaa aatcgctcac cctttttcac
    10801 ctgtttaaaa tatgttcagc aacccatctt gatggcgacc tcctctccgc gatgatttca
    10861 ataacatatt ctgtgttggc atatgaaatt ttgaggatta ccctacactt ataggagtta
    10921 ccttacaggg gttccttcaa tttgtgttga tttacgcgag ataacgctat ggaattaaag
    10981 gattattacg ccatcatggg cgtgaaaccg acggacgatc tcaagacaat caagaccgcc
    11041 tatcgtcgac tggcccgtaa ataccatcct gatgtcagca aagaaccgga tgccgaagcc
    11101 cgcttcaaag aggtcgctga agcctgggaa gtgttaagtg atgaacaacg tcgcgctgag
    11161 tatgatcaga tgtggcaaca tcgcaacgat ccgcaattta accgtcagtt ccatcatggc
    11221 gacggtcaga gttttaatgc cgaagatttt gacgatatct tctcgtcaat tttcggtcag
    11281 catgcccgcc agagccgtca acgccccgcc gcacgtggcc acgatattga aatcgaagtg
    11341 gcggtattcc tcgaagaaac gcttactgag cataagcgta ccatcagcta taacctgccg
    11401 gtttataacg cctttggcat gatcgaacag gaaatcccga aaacgctgaa tgtgaagatc
    11461 ccggcaggcg tcggcaatgg tcaacgcatc cgcctgaaag gccaggggac gccgggcgaa
    11521 aacggcggtc caaatggcga tttgtggctg gtgattcata ttgcgccaca tccgctgttc
    11581 gatattgtcg gccaggatct ggaaattgtg gtgccggtta gcccgtggga agcggcgctg
    11641 ggtactaaag tcaccgttcc aacactgaaa gaaagcattt tgctgactat cccgccaggc
    11701 agccaggccg ggcaacgatt gcgcgttaaa ggcaaaggtc tggtgagcaa aaaacagacc
    11761 ggcgatctgt atgcggtact gaaaatcgtg atgccgccga aaccggatga aaacactgcc
    11821 gcgctgtggc agcaactggc agacgcccag tcgtcttttg atccacgtaa agattggggg
    11881 aaagcataat ggctaatgtt acggtgactt ttactattac cgaattttgc ctgcataccg
    11941 gcatctctga agaggagttg aatgaaattg tcggtttggg ggtggttgaa ccgcgtgaga
    12001 tccaggaaac aacctgggta tttgacgacc atgccgccat tgtggtgcaa cgcgcggtac
    12061 gcctgcgtca tgaactggct ctggactggc cggggatcgc ggtgg
//
LOCUS       DAJTYH010000107        11941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTYH010000107 DAJTYH010000000
VERSION     DAJTYH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..709)
                     /gene="envY"
                     /locus_tag="OO609_003177"
     CDS             complement(<1..709)
                     /gene="envY"
                     /locus_tag="OO609_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6634498.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFY"
     gene            complement(892..1782)
                     /locus_tag="OO609_003178"
     CDS             complement(892..1782)
                     /locus_tag="OO609_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6634499.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(1783..4751)
                     /gene="nfrA"
                     /locus_tag="OO609_003179"
                     /pseudo
     CDS             complement(1783..4751)
                     /gene="nfrA"
                     /locus_tag="OO609_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            complement(4738..6975)
                     /gene="nrfB"
                     /locus_tag="OO609_003180"
     CDS             complement(4738..6975)
                     /gene="nrfB"
                     /locus_tag="OO609_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6634500.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7125..8567)
                     /gene="cusS"
                     /locus_tag="OO609_003181"
     CDS             complement(7125..8567)
                     /gene="cusS"
                     /locus_tag="OO609_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6634501.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(8557..9240)
                     /gene="cusR"
                     /locus_tag="OO609_003182"
     CDS             complement(8557..9240)
                     /gene="cusR"
                     /locus_tag="OO609_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6634502.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9397..10779
                     /gene="cusC"
                     /locus_tag="OO609_003183"
     CDS             9397..10779
                     /gene="cusC"
                     /locus_tag="OO609_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6634503.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            10803..11135
                     /gene="cusF"
                     /locus_tag="OO609_003184"
     CDS             10803..11135
                     /gene="cusF"
                     /locus_tag="OO609_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6634504.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            11151..11870
                     /locus_tag="OO609_003185"
                     /pseudo
     CDS             11151..11870
                     /locus_tag="OO609_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2872 a         3058 c         3134 g         2877 t
ORIGIN      
        1 tgtaaaacgc cttaaaaaca gagataaagt acgacgtgct gctatagcca cataattgcg
       61 ccacctgagt gatatttttg ttatccatca ataacatctg tacggcgtaa cgcatacgac
      121 actctgtgac aatctggcta tagctggtat tttcgttttt taatttcttt ttgagcaggc
      181 tggggcttaa acatagcgaa ctggcgacaa ttcgcagatt ccagtaatgc tgaatatcgc
      241 tttgaataat gcggcagacg ctgtcgcgga cgctgctgcg taagatatac atcagtaggg
      301 caataaaccg cgattgctca agaaagttag acaatacggt aaaaagtaat gcgcgcgtca
      361 actccgcttc gcagggtttg ctggattgct gcttgctgtg gttggtggct agccggaaca
      421 cttccggtgt acggctatga caagcgataa ccggggttgc tagccgcgac cagggcttta
      481 cacaggttaa tttcttattt aaaaacaaca gataatcgtt gatgatgtta cggttgatgt
      541 gggtgattaa tacgtgattt aacgttgaga gttcaatgac gttattgttg cacgcgaagg
      601 cggccaggta gtttttcgga agggtaaacg tagcatggtt attgacgctt acctctacct
      661 ctttttcggt caggatcacc acgcaaggtt cactgctgct caattgcatt tcgcactcct
      721 cagatatcag aaactccgct caaaggatct atgcttcctg catgagtgat cggcccgttc
      781 gccgataacg atcttctttc tttagcatgc tttttagcaa ttaatcttga tggaattctg
      841 acgagagcga aagaggtaag ccaggtcgta cccgacttac ctggagggga tttaatactc
      901 gagaatgccg tgcgcgacgg gcaaatagcg cagagagaaa tagagcatgt ctttaccgca
      961 agaggaacgt ttcgctaaca gcgaggcaat ttctgcgtcc ggtttcggtt tcgctgtaaa
     1021 ggttttgcct ttgccggcga caaacagttc ataaacaatg ccgcttgctg gggcggagct
     1081 ttggcaatcg gcgctggcct gtagataacg ttcacgctgt tcagcggtaa gtttatccac
     1141 gccgctgcca tcctgtaccc agacattaac tccggttgct ttaacttgtt ccagcagttg
     1201 gcgatagcca tcgggcgaca tgtttccggc gaaaaaacta ctgatataaa ccggttttgc
     1261 tgaaacatcg ctaatcagcc gctgcgcgtt gtttaaccat gttagcaaag gttgacgggc
     1321 ggcttcgctg cgccagttca ggtcgtcaat ttccgcgctg atgtaccagc catccggcgt
     1381 tatgccaggc gcggcgctcc ataatctggc ttgctggaga tcggcagcca gcaggcgatt
     1441 aagatagctt tccagcgctg cagacgactg tttctggtgc ataaaaaatt ccggatcggc
     1501 gttcagcccg acaatcagct tcaggccagt ctgttgcgca gctgcggcac gcttaaacaa
     1561 taacgtgcgc tgttctggct gggtaaatgc atcgccgtaa cgggtccatt gcaaaaccag
     1621 ggtatcgaag ccttgaaaac gtaactgact catcagcccc tgccactggg tatcggtaac
     1681 ctgactatct cggttttgtg gttgccagat aatacctttc atcgcaaagg aaaaagggct
     1741 gaccaaaagc agtgtcagca atacgaaaat gaacttacgc atttaccagt gcactccaat
     1801 ggtgagaaac gcgttgttgc gctctccgtt acgttgatta atcgccttaa aggtatgttg
     1861 atactcgacg ccgagactga ctttgtgcgg ccaggcgtcg tagtgcgtct cgccggtcca
     1921 aatattccag cggaccccga caccgccaag ctgcgcgccc tgagtgcctt tatcacgata
     1981 gccgttgtcc tgaacgtgag cgtaaggctc aatagtctgt ccgttagcta ccttctgatg
     2041 ccagctgacg cgataatctg ccgtccacgc ctgaatatcc tggcggatat attgcgccgc
     2101 atcgaggtac aggttttggg caaaccagcc tgaaccgttc gggtgccatt cgtcgctgta
     2161 tttgccgcca ttaaagaatg aggcgctggc gcgcagcatg gtatcggatg cgccattttg
     2221 cccgttcagc ggcaactgct gttcgacggc gaggaaaaag atctgatcgc gcagcggctt
     2281 ccagcgcaga ccggtgccgg acatcggatt tttcaccggc atcatcaccc cgttttctcc
     2341 ggtatcggca aagacgcggc tataaactga gagcaggtcg ccttccagca gcatattgcg
     2401 tccgatgcgg tactcggctt ccagttgtcc gtagctacga tagcttttcc caggcgctgc
     2461 accgccgaca ttattgttag cggtactcat tgcgccggaa cgcaagccga tggaagaatc
     2521 gaaactgaac gtccagcggc gaccgacctc ctcatgcaaa cggcggaaat tgaagcgttg
     2581 ctgattttgc tctggggtca gtggggttat gcctgattat caatgtcatc aatcacgagc
     2641 cgggcgtagt gctgcgtcgc aggcatgtca tccagacgct ggttcacgta ggccagttgt
     2701 cggatcagtg ccggatcgtc cggaagccct ttatgcgcct gttcgagcat ttcccgcgac
     2761 tgtgcgatat caccgctatc ccacaaggcg taaccaagcg ctgcctgggt gttgctatta
     2821 tttggttcca gttccagcgc ggcgcgcaaa tcactcaccg cggccggaac attatgacgt
     2881 tggcgataaa ttgtcgcccg cgcaacgtaa gagttggcag aaggggcaat attgattgag
     2941 cgcgtgagat cgttcagtgc gagttctggc tgagctggga tataacgtcg cgcatgcagc
     3001 caccagtatt gagcattgtt tcccagctca cgttgttctg cctgttgaag ccagcgatcg
     3061 cgagctgcgc catttcccgc tgcctgagcg gtgttggcgg cggcgagcag atcatcattg
     3121 ctcatgtcgt gaagactgat tttctgccag gccgccagtg cggtggcgta gtcctcaacc
     3181 tgatacgcct gataggctac cgcacgatgt tgccaggcgc tcggttgtcg ttgttcggcc
     3241 tgaagccatg catacaacgc cacaccgggt agcgtgtccc gataacactt tgccagacgg
     3301 ttccaggcgg cagcatcgta ggaaggcgac atatcgccca gcaagcgaac tattgccggg
     3361 caattatctg caatacccgg caactgactt tgccactgac gttgctccgc cagcggtaag
     3421 ggtttcgata aaatcgccac cttcgccggc gttgctaggt aaggatgact ttccagcaga
     3481 gacgccagtc gcgccattaa agtctggctg acacgcgcat cgccctggaa aggatagcgt
     3541 tgcagcaata aatcggcagc ttcgcgtgac tgctcgttct gcatcagttg ccaggtcagt
     3601 tgatccaggc ggggaagatt tgtcggttct tgctgataca gcaatcgtgc cagacgcaga
     3661 gattcagcct tgttacgggt cgccacgctg acagcataac gctcctcaag catttcattg
     3721 gcggggaggg tggcgagcag tttttgcgct gcgtcgtact gaccttcttt taacagcacc
     3781 ggtagcgtcg cgccaacaac atactggcgg ttatcggcaa actgcaccgt ataattcgcc
     3841 aatgcctgaa cggggttggc gctgtattta gataacagat agagccaact tttctcttgt
     3901 gcgcccgtgg taaacagcgg cttattttca atgagataat gctggaggcg tgctttttcg
     3961 ccacgataag ccagcgcggt cgcgtaagta atatatgact gaggatcggt gaagatcccc
     4021 tgtgattgca gtgccaggat ccgatcgtcc agctgcccgg caagaagcac gtcaaaccac
     4081 tgacggcgtt ctgccgcgct taatgtgttc tgctggcgtg cttcattgta tagcgtatct
     4141 gcctgggatc attgtttcag gtagattgcc cgttgcagca gatcggttcg cagcgttttt
     4201 ccttccggcg atgcagcaaa cgtcgcatcg ttcagttgcg ctctggcgac aggtaattgt
     4261 gccagccgca gggcattctg cccgacttca ctgcgacaac gcagggtcgg cgcagcgtcg
     4321 cccgcttttt gctgggcaag cagttcttca acagtcgtaa cgcttttcac ttcaactgga
     4381 atagccgcca gactgcgctc aagtcgggca tctcctgggt gacgtttcag ttgatcctca
     4441 agcaacagcc gcgcccggtc atcatgacca aaatgacgat aggcttccgc aaggtataaa
     4501 gtcagcggaa tattatccgg cacctgttgg tgtatatatt caaattcgcg gatggcggtt
     4561 gcttcgtcgt tatttttctg tgccttcagc gccttatcga gacggggata aataacaaaa
     4621 tggcgataat cgctcagccc cagctcttct gcgctggtgc cgatattgtc tgcgagtgcg
     4681 ctggtactca ataaagacgt cagcagtaaa ccagaccatc cgatgacgcg attaaggtta
     4741 ttctccttca ttttcggact ccagttgcgc aacctgttct gtgtttaaac ctgctttgag
     4801 taatagtgat tgcatcgaaa cttgtaattc gcgttgaatt gtcaggacgc gatccaacgt
     4861 ttcctggctg ataacgcctt cggtgaccaa aaacttgccg agcggcagag aactgcgttc
     4921 atggcgcaat aacaacacgt taattgctga acgattaata tgaccgagcg tggtcagtat
     4981 ttcggcgaac aggaactgat gcggcacata ttgccgccag atttcaccgg tctgctgttc
     5041 cgtgagccac tgatgctgaa ccgcattgta caacattgcc cgcggatcgt gaccgcgtcg
     5101 gcgtgcatac cagtggcgta accctgtgac aatttgtccc cgcagaacaa tgacgtaacg
     5161 cactttgcgt ccgactttac gcgtcagggc cgccagcgaa accgggtcaa taccatcttc
     5221 actgccgaca attaactcat cattttccag acgcagcggc agtactgcat aatgcagcgc
     5281 tacggaggct ggcatttcgg caatcagcga ggacgggatc tgccaggcat cgatggattc
     5341 ccacgccacg ccgttttgct ctgccagcgc ctgtgccagc tgctcggcgc taatcagccc
     5401 ctgcatcagc attgaaccgc ccaggcgtag accttcgacg cgattacgca gtgctgtatc
     5461 gagttgttct tcagtgatga cctgattttc cagcagaatt tgacctaacg ggcgcaacga
     5521 gcgggtatcg ccagtgacgc tggggaagtc atgcgttgtt ttatcccacg cgacgcgacg
     5581 cggatcgccg tgctgaagaa cctgtttcag cacgcgccag ttagccataa agttaatcag
     5641 gttgccccag aaaagacgca ggacggaaag caggccctgc gtgaggccgt agtagccagt
     5701 gacgaaaatc actcgctgca cgatgcggtt aaccatcaag ccaaagttta gccacagcag
     5761 ggtcattaac catgcgctgc cgctaaaaat agaaaggaaa tgccaggcat cgggccacaa
     5821 actttcatac gccagcaaca gcaaaagctg gatcatcacc agcatcgcga ggaagctgac
     5881 aaagttactg attgcccctt tgcggtcgcg ccagagaaag tagttcagcg tcaggctgga
     5941 ggtccattta tgggttttaa agccctggaa aacaatgccg atgatccagc gagatttttg
     6001 tcgaaccgca gtcgaaaagg tatcggggaa atattcgcgc acgcagatca tgtttgacgt
     6061 ccgtgcgtgc tgtaaaaatt tacgctgctc gcgttctttg gcttcgtcca ccaccggaaa
     6121 acggacaaaa atttccgtca tacctttttc tttcaggcgg aagccaatgt cgtaatcttc
     6181 agtaagactc tgcacgtcga aagcaatacc gttaccgtca gctaacagtg cggtcacggc
     6241 gcggcggctg aaacaggtgc cgacgcctgc gctgggcact tgtccggcga gggcttcacg
     6301 caccggaaca tctttgccat gcagttctga aaactcatca atgtaagtca tgctggtgaa
     6361 gtgcgtccat tcgcgttcaa acggatacac tgggatctga atcagatctt tacgctcgac
     6421 cagatagttg aacagacgca attccatcgg tgaaatcaca tcttcggcgt catgcagaat
     6481 aaaaccagca aaagcgaaat tggcgctacg ttcaaactgg gtgatggcgt ccagcacgtt
     6541 gttcaggcag tcggctttgc tggtgggacc aggacgcgcg cagactacct tatgcacgtt
     6601 cggaaagcga gcgcacactt cgtcaacatc acgttgagta tcggggtcgt tggggtaggt
     6661 gccaacaaag atatgatagt tttcgtagtc gagcgtggtc gccgccagct cggccatatt
     6721 gccgatgacg cccgtttcat tccacgccgg aaccataatc gctaacggtt tttcatctgg
     6781 tttatacagt tcgcggtaac tcattcgcgg gtagcggcga taaacactca acttgcgttt
     6841 aatgcggcgt acccagtaga cgacatcaat aaaaaaatcg tccagcccgc tgatgaacat
     6901 gatgaccgct aacgttatcg cgattacttt taagccgtag agccaggtag caaaaacatc
     6961 aagaagccag tccacacaaa aaccttacat taacgctggt tatgtttagg gtggcgtata
     7021 ttaaggtttt ttatgaattg tgacagcttt ttaccattaa tatgtatgac tattgcggca
     7081 cgttattttt acactggtta taaaagttgc cgtttgctga ataattaagc gggtaatgtg
     7141 ataacaaacc ttgtcccccg cgcatctgac gttaccgcaa ccgtgccttt atgcgcgaca
     7201 acaatcgatt tcactatcgc cagcccaata ccgctacctt caccttttcg ctggcgggag
     7261 gggtcaacgc gatagaaacg gtcaaacaat cgcggtaagt gctcgggcgc aatgggcgta
     7321 ccggggtttt cgacggaaac ttgcacctgg tgatcgaccg tctgacagcg tactacaatt
     7381 gtctctctgg ttggcgtata acgaagggca ttagaaagca ggttgcttag cgcccgacgc
     7441 agcatcaacg gatcgcccgc aacctgacgt ttgtcgccaa caaaccgcaa ctccacgccg
     7501 cgatcttccg ctagcgcctc gaaaaaatcg aacactttgc cgacttcatc cgccaggttg
     7561 agcattttct tttcggggat tagctggttg ttatcggcct gagcgagaaa cagcatatcg
     7621 ctgaccattt tcgccattcg cgtcagctct tcgagattag agtagagcac atcttccagc
     7681 tccttctggc tgcgcgactg gctgagggcg atttccgttt gcgtaatgag attagtaatt
     7741 ggtgtgcgaa tttcgtgggc gatatccgct gagaaattgg actggcgagt aaagacatcc
     7801 tcaatacgct cgatcatatg gttgaacgac agtaccagct gttccagctc aatcggcacg
     7861 gtctgcgggt caaggcgaac gtcgagatct ttcgaggtga tattctggat ttgacggctg
     7921 acgctgcgga tcggcgcgtg acctttatgt accgccaaca gcacgataaa gacgatcagg
     7981 atgctgatta ccgatgcggt cataataagt ttattcatca aatcatttat gtaatgaaga
     8041 tgaaaatcga tcgacagcgc gatgtagagc gtataaatcg gtttgccgtc caccaacggg
     8101 ccaaccggca agttaatcat ccgccagttg ctgtgttcca tatgcccgtg accgtggcct
     8161 ggcatcataa tcgtcgggcc ggaaaggaga tacacctcgc caccccgcgc gtctttatcg
     8221 ggtatggcat cacgtgcaaa ctcgcggata tcaggcgcac cgggggagtg atacaccgtt
     8281 ttgccgtggc tatctgccag ggaaatcaac acgttggaat aaccactgac gatatcttcc
     8341 agcgtcatta agcgtcgggc ttgcgtttcg tcagggtgat ttagtacccg ttcaagggtg
     8401 gcgctaatct cttttaaatc attaatgtcc tgctcggcaa aatgcacttt tactgagtgg
     8461 attatgatcc atgcaaaggc gaaaaacgcc gcgatggtgg cctggctgat aaaaaaggtc
     8521 aggcgggttg ccagcgaaaa cgggcgctga aatggcttac tgaccatccg gcacctcaag
     8581 catgtaaccc acgccgcgca cggtttgaat cagcttcggc tcaaagtcgt tgtcgatttt
     8641 gccgcgtagc agcttcaccg ccacatcaat ggcgttagtg tcgctgtcga aattcatgtc
     8701 ccatacctgc gaggcgataa gcgagcgggg cagcacttcg ccctgatggc ggagaaaaaa
     8761 ctccagcaga gtaaattctt tactggtcag agtgatgcgc gtgccgctgc gggtgacttt
     8821 gcggctgacg agatcgacca tcagatcggc aacctgaaac tgactttcga taatcaccgc
     8881 cgccccgcgc cgcaggagag tgcgcacccg cgccagcagt tcagcaaaag cgaacggttt
     8941 caccaggtag tcatctgccc ccaactccag ccccttgacg cgatgttcaa tggtgccaag
     9001 cgcggtaagc aacagaatcg gcatcccttt attggcggag cgcaacatgc gcacgatatc
     9061 ccagccgttc acgtctggca gcataatatc gaggattatc aggtcataat caccggtcat
     9121 cgccagatgg tagccattca gcccgttgtc ggccaaatcg accacaaaac cggcttcggt
     9181 taaccctttg gtcaagtatt ctccggtttt cttttcatct tcgacaatca acagtttcat
     9241 atttcctccg catgttgccc gggcaattct agagtagcgg gatcagatgg caatcgctta
     9301 ttggcaaaat gacaattttg tcattttcct gtcaccggaa aatcagagcc tggcgagtaa
     9361 agttggcgac ataaaatcac cagaaattat gagcctatgt ctccttgtaa acttctgcca
     9421 ttttgtgtgg cccttgcgct aactggttgt tcactggcac cggattatca gcgtccggta
     9481 atgcccgtac cgcagcagtt ctcactcagc cagaacggcc tggttaacgc agcggataac
     9541 taccagaacg tgggctggcg cacctttttt gttgataatc aggtgaagac gctgattagc
     9601 gaggcgctgg tgaataaccg ggatttgcgc atggcgacgc tgaaagtgca ggaggcacgg
     9661 gcgcaatatc gtctgaccga tgccgaccgc tacccacagc tcaatggcga gggcagcggc
     9721 agctggagcg gcaatcttaa aggcgattca gccacgacgc gggagttctc gactggcctt
     9781 aacgccagct ttgatctcga ttttttcggt cgcttaaaga acatgagcga agccgagcgc
     9841 caaaattatt tagccactga ggaagcccag cgcgcggtgc atattttgct ggtttctaat
     9901 gtcgcgcaaa gctatttcaa tcagcagctg gcgtatgcgc aattgcaaat agccgaagaa
     9961 acgctgcgta attatcagca gtcatatgcg tttgtcgaaa aacaactgtt gaccggtagc
    10021 agcaatgttc tggcgctgga acaggctcgc ggggtgatag aaagtacccg cagtgacatc
    10081 gctaaacgtc agggggaact ggcgcaggcg aataatgcat tgcaactgtt attgggaagc
    10141 tacggcaagc taccgcaagc gcagacagta aacagcggca gcctgcaaag cgttaaatta
    10201 ccggcgggct tgtcgtcgca aatcttattg cagcgcccgg atattatgga agctgaacac
    10261 gcgttaatgg cggctaatgc taatattggt gctgcgcgtg cggcattttt cccgtctatc
    10321 agcctgacca gcggaatatc aaccgccagc agcgatctgt cgtcattatt taacgctagc
    10381 agcgggatgt ggaattttat tcctaaaatt gagatcccca tttttaatgc cggacgcaac
    10441 caggccaatc tggatatcgc cgaaattcgc cagcagcagt cggtggtgaa ttatgaacag
    10501 aaaatccaga acgcctttaa agaggtggca gatgcgctgg cattacgtca aagcctggac
    10561 gatcaaatca gcgcccagca gcgttatctg gcgtcgctgc aaattaccct gcaacgggcg
    10621 cgggcattat atcagcacgg cgcggtaagt tatctggaag tgctggatgc cgagcgttct
    10681 ttatttgcaa cccgacaaac tttacttgac ctgaattatg cccgtcaggt taacgaaatt
    10741 tatttgtata ccgccttagg gggcggttgg cagcaataat ttttaactcc aggagagaat
    10801 aaatgaaaaa agcactgcaa gtcgcaatgt tcagtctgtt taccgttatt ggctttaatg
    10861 cccaggctaa cgaacatcat catgaaacca tgagcgaagc acaaccacag gtcattagtg
    10921 ccaccggtgt ggtaaaaggt gttgatctgg aaagcaaaaa aatcaccatc catcacgatc
    10981 cgattgctgc cgtcaactgg ccggagatga ccatgcgctt taccatcacc ccgcagacga
    11041 aaatgagtga aattaaaacc ggcgacaaag tggcgtttaa ttttgtccag cagggcaacc
    11101 tttctttatt acaggatatt aaagtcagcc agtaacccag gtttaatgag atgaaaaaaa
    11161 tcgcgcttat tatcggcagc atgatcgcgg gcggtattat ttcagcggca ggttttacct
    11221 ggtttgacca ggcggaaccg cacgcagaaa aaatgccgac cgcagaacgt aaaatcttat
    11281 tctggtacga cccgatgtat cccaatacgc ggttcgataa accaggtaaa tcgccgttta
    11341 tggatatgga tctggtgccg aaatatgccg atgaagagag ttctgcgtct ggtgtgcgca
    11401 ttgacccgac tcagacgcag aatctggggg tgaaaacggc taccgtcacg cgcgggccgc
    11461 tgacttttgc ccagagtttc ccgacgaatg tcagttacaa cgagtatcag tatgccattg
    11521 tgcaggctcg cgctgccggg tttatcgaca aggtgtatcc gcttaccgtg ggcgataaag
    11581 tacaaaaggg cacaccgctt ctcgacctga ccattcccga ctgggtggaa gcgcagagtg
    11641 agtatttact gttgcgcgaa accggcggta cggcgaccca gactgaaggc attcttgagc
    11701 ggctgcgact ggcgggaatg ccggaggcgg atattcgcca cctgatcgcc acgcaaaaaa
    11761 tccagactcg ctttacgctc aaagcgccca ttgatggcgt gatcaccgcg tttgatctgc
    11821 gcgcgggaat gaatatcgcc aaagataacg tggtcgcgaa agaggaaggt aggttgctta
    11881 tctgtgtctg tcattgtcta gcattgtcca ctgtagttaa ttaactgtct gttataactc
    11941 g
//
LOCUS       DAJTYH010000108        11899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTYH010000108 DAJTYH010000000
VERSION     DAJTYH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..219)
                     /locus_tag="OO609_003186"
     CDS             complement(<1..219)
                     /locus_tag="OO609_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6634505.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSI"
     gene            460..1260
                     /locus_tag="OO609_003187"
     CDS             460..1260
                     /locus_tag="OO609_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6634506.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1338..2108
                     /locus_tag="OO609_003188"
     CDS             1338..2108
                     /locus_tag="OO609_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6634507.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2156..3502)
                     /gene="mltD"
                     /locus_tag="OO609_003189"
     CDS             complement(2156..3502)
                     /gene="mltD"
                     /locus_tag="OO609_003189"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6634508.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(3574..4329)
                     /gene="gloB"
                     /locus_tag="OO609_003190"
     CDS             complement(3574..4329)
                     /gene="gloB"
                     /locus_tag="OO609_003190"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6634509.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            4363..5085
                     /locus_tag="OO609_003191"
     CDS             4363..5085
                     /locus_tag="OO609_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6634510.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5082..5549)
                     /gene="rnhA"
                     /locus_tag="OO609_003192"
     CDS             complement(5082..5549)
                     /gene="rnhA"
                     /locus_tag="OO609_003192"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6634511.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            5614..6345
                     /gene="dnaQ"
                     /locus_tag="OO609_003193"
     CDS             5614..6345
                     /gene="dnaQ"
                     /locus_tag="OO609_003193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6634512.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            6478..6554
                     /locus_tag="OO609_003194"
     tRNA            6478..6554
                     /locus_tag="OO609_003194"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6512..6514,aa:Asp,seq:gtc)
     gene            complement(6685..7731)
                     /locus_tag="OO609_003195"
     CDS             complement(6685..7731)
                     /locus_tag="OO609_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6634513.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(7745..8304)
                     /gene="tssG"
                     /locus_tag="OO609_003196"
                     /pseudo
     CDS             complement(7745..8304)
                     /gene="tssG"
                     /locus_tag="OO609_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8301..10184)
                     /gene="tssF"
                     /locus_tag="OO609_003197"
     CDS             complement(8301..10184)
                     /gene="tssF"
                     /locus_tag="OO609_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6634514.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10200..10694)
                     /gene="tssE"
                     /locus_tag="OO609_003198"
     CDS             complement(10200..10694)
                     /gene="tssE"
                     /locus_tag="OO609_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6634515.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(10691..11521)
                     /locus_tag="OO609_003199"
                     /pseudo
     CDS             complement(10691..11521)
                     /locus_tag="OO609_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(11508..>11899)
                     /gene="tagK"
                     /locus_tag="OO609_003200"
     CDS             complement(11508..>11899)
                     /gene="tagK"
                     /locus_tag="OO609_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HCR6634516.1"
                     /translation="YREALLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLVTEAETLQ
                     DMVTGGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNTVLPDLTR
                     KEHRIIGIDSHYRINPAQHGEIYHDKQ"
BASE COUNT         2714 a         3109 c         3199 g         2877 t
ORIGIN      
        1 aattgactgt acgcgacgaa aataacgctc gccttcttcc gtcaggctaa gttgccgcgt
       61 ggtccgatta agcaggctaa cgccaagttt catttccagc tttttcaccg cccggcttac
      121 cgctgagttt gcttgcccta attgttccgc tgcccggcta aagctgccgc tttcgacgac
      181 cgaaacaaaa atggcgagtt cttccgacgt ggctttcatt tttgctcctg ttgcaaaata
      241 gatgagatat tttgaattta tttgtcatta aaccatcagg atgcgtgata tgtcatccga
      301 tttaatgttc tcaataatga gcaaatttct gacaggtgta agcacttgct tacataacaa
      361 tatacaattg ctcgttgaaa gagtgagcta aaatccctat aacagtagaa ccctcccgag
      421 tgcggaaggg ttgacgtaat agaggtttca aagtcaaaag tgcgaaaaaa cacctatgcc
      481 atgcgctatg ttgccggaca acctgcggaa aggatcttac cgccggggtc ttttgcgagc
      541 atcggccagg cattaccacc tggagaaccg ttaagtaccg aagagcgtat tcggatcctg
      601 gtgtggaaca tatacaaaca gcaacgcgct gaatggttgt cggtattaaa gaactacggc
      661 aaagatgcac atctggtgtt attgcaggaa gcgcagacaa cgccagagtt agtacagttt
      721 gcgaccgcta actatcttgc cgccgatcag gtgcccgctt tcgtgctgcc acaacatcct
      781 tccggtgtaa tgaccctttc ggcggcgcat ccagtgtatt gctgcccgtt acgtgaacga
      841 gaacccattt tgcgtctggc gaagtcggca ctggtgacgg tctatccatt acctgacacc
      901 cgcctgttga tggtggttaa tatacacgcc gtcaacttca gtctgggcgt ggatgtctat
      961 agtaagcagt tacttcctat tggtgatcag atagctcacc acagcggccc ggtcattatg
     1021 gcgggagatt tcaatgcctg gagccgtaga aggatgaacg cgttatatcg ctttgcgcgg
     1081 gaaatgtcgc tgcgccaggt gcgttttacc gatgatcagc gccgtcgggc gtttggccgc
     1141 ccgctcgatt ttgttttcta ccgtggtctg aacgtcagcg aagcttctgt attggttacg
     1201 cgcgcttccg atcacaatcc gctactcgtt gaattcagtc ccggcaagcc tgataaataa
     1261 ggtatgtcag gtctgccaca gggcagacca acgtttggcg ctgtcctttt atactcaaac
     1321 aacaaaagga cagcgcaatg acaacacaat cccaccatga ccacgtagaa aagcagttta
     1381 gctctcaagc ctgcgaatat ctgaccagta ccgtacatgc atccggacga gatttacagc
     1441 gcctggcggt gcgtctggct gattatcctg gtgcaagtgc acttgatatg ggctgcggag
     1501 cagggcatgc cagctttgtt gctgcgcaaa acgtgagcgc ggtggtggcg tatgacttat
     1561 ctgcccaaat gctggatgtc gtggcacaag ctgccgaagc ccggcaactg aaaaatatca
     1621 ccacccgcca gggatatgcc gaaagtctgc catttgccga taacgcattt gatattgtta
     1681 tcagccgtta ttctgcccat cactggcatg atgttggtgc agcactgcgt gaagtgaacc
     1741 ggatattgaa acctggcggt aggatgattg tgatggacgt aatgtctccg ggtcatccag
     1801 tgcgcgacat ctggttacag acggtagaag cattacgcga tacctctcac gtacgaaact
     1861 acgccagcgg tgagtggttg aggttaatca atgaagccaa tctgatagtt gataatttaa
     1921 ttacagataa gttaccgctg gaattttgtt catgggtcgc gagaatgcgt acgccagaag
     1981 cgttagtaga cgctattcgc atttaccaac agagcgcatc gacagaggtg agaacgtatt
     2041 ttgccttgca gaatgacggc tctttcacca gtgatatcat catggtagaa gcacataaag
     2101 cggcataaat aaaaaaggca ccgggggaat cggtaccttt ttattatctg gtttgtcagg
     2161 aatctggcat gttgttgttt ttcacaaaca acgtcagctt gtcgcctggt tgcagattcg
     2221 cagtgtcgct gttccagcgc atcacatctt tgatgttcac gccgtggcgt ttagcaatgc
     2281 ttgaaagcga atcgcctttg cgcacacgat acgtaatgct atcgctgttg tttgccagtc
     2341 gctgtgcacc aatcgtcaaa ctttggcctg gcttcagctt agatccgcgc agtttgttcc
     2401 actgctgcaa atctttggtg cttacgccga gacgtgaagc gatgcttgaa agcgtgtcgc
     2461 cagagcgtac ggtgtaaaca cggctgttaa gcggcgtatt gttggcaacc agcgtcgact
     2521 gtacagcagc aatttcgcct gaagcgagag attcacgcag ttgatctgca tgcttctttg
     2581 gcaccatcac gtactgcggc ccacttgcgc ccagcgtgga gcctttcacg ccagcgttga
     2641 atgtcttcag cttgctgacg gaaatccccg ccatatctgc aaccttcgcc atttcaaccg
     2701 ggctactcag gtgcacacgc gccagagcac ggctttcatc ggtcgttggc agacgtacgc
     2761 cataacgctt gctgtttttg agaatatcac tcaatgccag cattttaggc acgtactgct
     2821 tcgtttcctg cggcaacggt aacgaccaga agtccgtgga tttcccacgc gctttgttcg
     2881 ttttaattgc cttcatgacc cgaccttcgc cgctgttata agccgctacg gtcagaagcc
     2941 agtcgtcgtc aaacattttg ttcagacgct gcatcatatt cagcgcggca gttgttgaag
     3001 caacaacatc gcgacgcgcg tcataattgc gggtctgttt caaaccataa ttgcgccccg
     3061 tgctcggaat gatctgccag atgcctgcgg cattggcgcc agacgttgcg tgaggatcaa
     3121 aagcgctctc cactatgggt agtagtacca gttccatagg catgttacgt tttttaactt
     3181 gccctgctat ccagtacata tacggctctg cccgtaaagt tacatcgtgg agatagctct
     3241 tattgcgtaa atatttctgt ttctgttcgc gaatccggtc attttccgga attcccatct
     3301 ttagctcgtc gccaatgaaa gcccacaagt caccatctgg cgcgatagac gtcccatcgt
     3361 ccatccatcg tgcctgactt gtaaactttg ctgcttcccc ttgaccagct gcagaaaggc
     3421 tctgtgcgtg ctgttgaacg ttgccggtac tctggcaacc cacgagcagg acagaggcga
     3481 gtaatatcgc ttttgccttc atgtgtgtgt caatagttgc ttaaaagacg accgatcata
     3541 acggcgaacg gagccgatga caagaaagtt ttatcagaac ctatctttct ttgaccttaa
     3601 ccatgcaaaa cgctcttcag gttgttgcaa taatgtttct tcattaattc cattaattaa
     3661 atcaatatct tccgttctta aaaaaacatt aatttgccgc tcatttttca gaattacggg
     3721 tagtgttatt tgattttttg cccgtaactc cttaacttta cgataataat catttatgga
     3781 caaatcgtgc ggaaaaatac tcaaagcaaa cttcatattt gataacgtat attcatgagc
     3841 acaacatacc aatgtatcgt caggtaacgc acttagcttt ttaagtgatt gatacatttg
     3901 taaagctgtc ccttcaaaca accgaccaca cccaccagaa aacagcgtgt cgccgcaaaa
     3961 tagataaggt ttactgaagt aacagatatg tcctaaagtg tgacccggtg tggtaattac
     4021 actaaattca tgccctaaaa cgaaggcagt ttcgccatct ttgactacct gtgttgttcc
     4081 cttatcttgt gtctcttgtg gaccatacac tacaatttgc ggaaactttt ccaccagttc
     4141 ttttacgccg ccaacgtgat cgtggtgatg gtgggttaga aatatggcct ccggttgcca
     4201 gttattggcg gtaatggcgt ttaatactgg ctctgcgtct ccgggatcga caatcaggca
     4261 gcgacctgcc tcatcattca aaacccagat gtaattgtca tcaaaggcgg gaatactgtt
     4321 aagattcata gattacctct cagtgtaaaa cggaaggttg tgatgaaacc ggcaagagtc
     4381 cctcaaactg tcgtggctcc tgattgctgg ggcgatttgc cctggggaga gctttatcgc
     4441 aaggcgctgg agcgccagct caacccgtgg ttcactaaaa tgtatggttt tcatctgctt
     4501 aagattggca atttaagcgc agaaatcaat tgcgaagcgt gcgcggtttc tcatcaagtg
     4561 aatgtttctg cgcaaggaat gcccgtccag gtgcaggcgg atccacttca tcttcctttt
     4621 gccgataaat ccgttgatat ttgtctactg gcacatacct tgccgtggtg caccgatccg
     4681 catcgtttat tacgtgaagc cgatcgggta ctgattgatg atggctggct ggtgattagt
     4741 ggcttcaacc ccatcagttt aatgggatta cgcaaacttg tgccggtatt gcgcaaaacc
     4801 tcgccctata atagccggat gtttactctg atgcggcaac tggactggct ctctttgttg
     4861 aattttgaag tgctgcacgc cagccgtttc cacgttctcc cgtggaacaa acacggagga
     4921 aaactattga atgcgcatat tcctgcgctt ggttgcttac aacttattgt tgcccggaaa
     4981 cggactattc ctttaacgct aaatccgatg aaacagagta aaaacaagcc acgaattcgc
     5041 caggcggttg gagccacccg gcaatgtcgt aaaccacagg cttaaacttc gacttggtag
     5101 cccgtatctt ccagtgtggg attcatcgcc gcggcacgag ccagttcatc acagcgttcg
     5161 ttttccgggt gtccggcatg gcctttaacc cattcccatt tgatttgatg ctgccccaat
     5221 gcagcatcaa gacgttgcca gagatcgaca ttttttactg gttttttgtc tgctgttttc
     5281 cagccacgtt ttttccagtt atggatccac tgggtgatac cctggcggac atactggctg
     5341 tcggtactca aaatgacttc gcaatgttct tttaacgcct ccagcgcgac aatagcggcc
     5401 atcaactcca tacggttgtt ggtggtgcgg gtgtagcctg cgctaaaggt tttctcgcgt
     5461 ccgcgatagc gtaaaatagc gccgtaaccc ccaggtccag gattacccag acacgaacca
     5521 tcggtgaaaa tttctacctg tttaagcatc tctggtagac ttcctgtaat tgaatcgaac
     5581 tgtaaaacga caagtctgac ataaatgacc gctatgagca ctgcaattac acgccagatc
     5641 gttctcgata ccgaaaccac cggtatgaac cagattggtg cacactatga aggccacaag
     5701 atcattgaga ttggtgctgt tgaagtagtg aaccgtcgcc tgacgggcaa taacttccat
     5761 gtttatctca aacccgatcg gctggtggat ccggaagcct ttggcgtaca tggtattgct
     5821 gatgaatttt tgctcgataa gcccacgttt gccgaagtag ccgatgagtt tatggactat
     5881 atccgcggcg cggagttggt gatccataac gcagcgttcg atataggctt tatggactac
     5941 gagttttcgt tgcttaagcg cgatattccg aagaccaata ccttctgtaa ggtcaccgat
     6001 agccttgcgg tggcgaggaa aatgtttccc ggtaaacgca acagcctcga tgcgctatgt
     6061 gcccgctacg aaatagataa cagtaaacga acgctgcacg gggcattact cgatgcccag
     6121 atccttgcgg aagtttatct ggcgatgacc ggtggtcaaa cgtcgatggc ttttgcgatg
     6181 gaaggagaga cacagcagca acaaggtgaa gcaacaattc agcgcattgt acgtcaggca
     6241 agtaagttac gcgttgtttt tgcgacagat gaagagctgg cagcacatga agcccgtctc
     6301 gatctggtgc agaaaaaagg cggaagctgc ctctggcggg cataaataca tgtaaaaggc
     6361 gctaaaaata gcgacttggg cgatttttgc agcaaacgat tcaaaagatg agaaaaatcg
     6421 ttgacgaagg tcgaggcaat ccgtaatatt cgcctcgttc ccaacggaac acaacgcgga
     6481 gcggtagttc agtcggttag aatacctgcc tgtcacgcag ggggtcgcgg gttcgagtcc
     6541 cgtccgttcc gccaatattc agaaagcccg aatcagtaat gattcgggct ttcgtcgttt
     6601 tggtgaagtg agaatttttc actccgaagt aaaacggata tgatttaaaa aatggccaga
     6661 cattataaat tctggccact tctgttactc cagcgcatcc catttctcca gcagttctac
     6721 tggaatttca tttattacct ccgagacgcg ttttccgacc aggcgttctg cctggcgcag
     6781 caaaggaatg gtcgggctgc tcggttcgct gtgctcaaac cagcgacgga tgacacgcag
     6841 gcgttccagc gcgtcgtttc ggtcgcggat ctgccggggt tctgcgccgg tcatcggtga
     6901 tatttcctcc gcgatatgca ccggtagcgc aatctgctct gcctgcatct gttcagtatg
     6961 aacgatggtg gcgttttccg gctgctcagc ctgtggttgt ggcaggatct ccggcgtggt
     7021 gctctgcact gctcccggta acagggccag cagttgcgtc agacgggaga agtccggtgc
     7081 ctggtcattc agcgtttcac gcgcatggcg ttgcagccgc tcagtggtct ctgctgcctg
     7141 gcgaaaggca tccagcggca gtgtgccgcg cgcttccagg tctgccagtt gctggcgtac
     7201 cgattctggt gccagcgcat ccgccgggcg taatgcagat agcgaacgct ccacatcccg
     7261 cacctgaagg cgcatggcag cattatttga cagtgtaatg ccacgaatat ccgccattac
     7321 accttcgtgg tccagcaacg ccgccagcgc gttactgcgt gccaccgcag catcttcggc
     7381 ggtgatatct tcagtcgcga gcagttgagg atggatggcg tccgggtacg tggagcacaa
     7441 cgtttccagc agcgtgagcg cctccgacag tcccctggca ccggcctgct ggatacggca
     7501 gcgcagcagg acaaccagaa tgcggatatc tttgctgcgc atcagcagac gatgggcgtc
     7561 ccgctcaagc tccgcccagt taatgttttc cggcgtactg acaaaatcgc catattgcgc
     7621 ttcttcacgc ggtgcggcgc gggtgaacag cagcaggtat tccggatcgt actccagatc
     7681 cgggccgcag gggcattccg ccgaaattgg gtcagacagg aatatttcca tttaattatc
     7741 ctcagtgcgc cagcgtggtg gccagaccgt ccggattgct ggactcacgc accagatggg
     7801 ccgatgccgc cagacgagcg tgatctggca acgggctgtc agcgctctcc cgcaaatcct
     7861 gcccgctgag gctggcgaca taaaaggcaa agcggtcatg ttgcggttta tccagaccgc
     7921 ccccagcgga ctgggcgctg cgccatgcct ggtaaaactg cgtcagccag cggtgatgaa
     7981 aaagatcaga aaaacgggtc agcgtcggct ttcagtccgg ttacgcgcca gttcggtgta
     8041 gtgcagcggc agtggaccgt tcggccccca cagcacgagg ctgtacagac tgatgtgcag
     8101 gcgcccttcc tgccagccca cactggcgat ttctcgtggc gcaaaggtca gcgcgggcgt
     8161 ttgtccgagg cgaaattttt ccatccgtgg ttgccaggtg ttgcccgccg gaacggtaca
     8221 caactgcaca tccacgcggc gcatcaggtt cagaaaaccg taacgccagg ggggggcctg
     8281 cgcctgagtg aaatcgttgc tcatacgctt ccccgctgtc ccgccctgac aggccaggtc
     8341 ataattttgc cgcgctgcat gctgtgcagc gtcatctgcg agaaggtatt aatggaaaca
     8401 tggcgggcga tatagtgttc cagcaccagg ccgaacagat acggactgat gccggaaaaa
     8461 ccttcttcat ccaccgtcag ttcacaactg acgccacgcc catagaccag tagtccggaa
     8521 ccgggcagac ggcgggtgac cggggtcgtt ttacaaccaa tcaggctgcg cacctggcgc
     8581 gattgcggac tgtcatgagc aggaataaac aggttcagca gatctcgcaa cgcctggccg
     8641 ccggtacggt gatccagatc ggccagcggc agataattaa aagataactg gcggatcaac
     8701 cgccatgcca tctcacgttc agccatcggg ggctgcggcg agcgtggcgg gcggataagc
     8761 cctacgcccg ccaccgggat ggctgcatcc acggttagat cgtcccggcc attgcgggca
     8821 ataagacagg gcagatcgcg gttagtgacc atggccgtga tggtgatgtg gcgcagattt
     8881 tccgggtacg gcgcttcata ctggtcaacc agcgacagga acacttccga gcccgtatag
     8941 ggggtacggg tgccgtaacg gcgggcgttt tctgataagc ggcggggctc ccggcgcagg
     9001 gaaaaataac gtccgtgatt gccttcatca ttattgcggg tgtggtagag cgggcgaaag
     9061 gccatcttcc gtgtggtgtc ggtttccagc ccttccactt cctggaccga gaacacctca
     9121 taatccagcg gatgggttct gtccaccacc agatgctgtt ccgtcgtgct gtgcgtgaca
     9181 tcgatccggg cggtgacgcg gggaaacagg ttgataaccg gggtacagaa caggctgaac
     9241 tgtgccgcat ttgtctggtg gatcagccag tccggcggca ggcggttcag caggataaca
     9301 atctccgcca cgccaccgtc aattttctgt aatccggcag acaggcctgt cggggtgaaa
     9361 aaatagaagc gttccggaca ggcaaaatat tcatgcagca ggttatgacc gtgaaacacg
     9421 ttccacgcca gcggcagcag cccctgatcc ggctccaggc cttcgtacat aacgggctgt
     9481 tgcagattca catccagcgc gccgtcaaaa tgccccggaa tgccagccag gggcgcgatg
     9541 gcgctggtat gtagaagttc taacaggtgt gaggccgtac gctcctcgcc gcagagataa
     9601 aacggcagcc tgtccagtcc ggcaagctga ctgaacgtta gttcaccaaa ggtgcgcagg
     9661 gtaatacgca gcgccccggc gacgtgaatg ttggccggca ggtagcggtg cagggcaggc
     9721 atatccggtg gcgcggcggt cagtcgcgcc tcttcgatgg cgagcggcca cagcgttaca
     9781 tcctggctgc tgcgaaactg gcaggcagtg ctttcgcctt ccggaatggc ggagaaaaag
     9841 gctgtgtcgc gcgggacggt aaatcctttc gccagatcgc cttcttccgt atcgggatgc
     9901 aactgcgcca cgctcattga gggcgtgggt gtgacgtaat tggggctgac cacttccagc
     9961 aggcgctggg tgaaacgggg gaattcagcg tcaattttta gctgcgtgcg ggcggtcagg
    10021 aagctgaagg cctcgaccat tcgctctaca tacggatcgg caatatccgt tccctgcatg
    10081 cccagccggg cggcgacttt gggatgacgg gcggcaaatt cggctcccgt ttcccgcaga
    10141 tagctcagtt cgcggttgta gtattccagt agccgtggat ccatcggtct ctctctgaat
    10201 taaaaaaacg taatgcgact ctgctccata tccagcgcgc tacgtacccg gaatgcagtc
    10261 ggatacggct gcgtgaggat ctcgccgcga atttcaaact gtaatgtgtt atggccactc
    10321 tggcggcgct catcctgaag tgggatgatt tgcagagtgg acgctttcag ccggggttca
    10381 aaccgggtga tggttcgcaa aatggccctg cggatatcgt cccacttgtg ctcatacaga
    10441 aagctgcccg ccagtggcgg caggccgtag ttaagtacgg acgctgccgc gtacggataa
    10501 cgtgcagcat caatatcatc ttcatggctg acggtgttga gcagaaatga caaatcccgg
    10561 cggatgatct ccttaatctg aaccggactg acgctgatac cgtggtcgcg tttctggttt
    10621 ggggcgtcgt cacacaggcg gtcaaacagc gtgggcagca ggtgattgcc tggacgctgg
    10681 cgggaagcgc tcacgcgtcg cttccttcag cggtgttaaa ggtacaggtg gacaaatcca
    10741 gcaggctgat atcgccgtga ccggtgagcc acaccctctg acccagcgcc tgtaccagtg
    10801 tctctcccgg accgtcctgc cagaccgttt cccggcacag acgcagggag tcggcagcct
    10861 tttcagagcc gctgtagcgg ggaaacagct aggcactgtg tgtatcgcca ttgttcaggg
    10921 tgacgttgac gggtttccag atcaaatcga tcagatgcgc aggccgcggc gatttcagcg
    10981 aacagatttg tgaaaacggc agccagatat atgctccgcc cgcgatcagt tcgagtaccg
    11041 gaccggtacg ggagtcgctg tcgctaatcc agtcaaatgc gccgccattc cactgcccgg
    11101 aagtgtctgt tatggcctca agcgcgcggc tgcggtgagc atcagcttcc tgcgcttcac
    11161 cacgggcgtt acaggcgagg gcggtcagca gatcctccat ccaggcgggc ggcgggagaa
    11221 taacaccggg ccgctgttcg ccctgaaagc aggcgtggcg gtatatctca cagcggatca
    11281 gctcgccaaa tacctgcgct tcgcgagtat aatttgcgtc catccgggcg catagctgga
    11341 tctgttgcag tgcccgcgac cagtcgcctg ccacgcacaa taactgaaac aggctgtggc
    11401 ggcgggaggc ctgcgctggg tgcatcctga cctgttgctc tgccatctga atgccctcca
    11461 ccacggaata atcccgcagc agcgccgaca aggtaatcgg tagctcgtta ttgtttgtca
    11521 tgatatatct ctccatgttg tgccgggttt atccggtaat ggctgtcaat gccaatgatg
    11581 cgatgctctt tccgtgtcag atctggcagt acggtgttat gcgctgtttc cggggcgtat
    11641 tccggggaaa gtaaatgcag gatatcaggg ggagtctttt cgacaggcca gatagtttca
    11701 tcttcgccag tggcatccag tacattgaga atagcgtcaa tgcttaaccc accagtgacc
    11761 atatcctgca atgtctcggc ttcggtgacc aggtcacgca atgaatccag atcctgcgag
    11821 gagggctctt cgttaaaagg aaaaggatca gcatggcgat taagatcttg ttcgggagat
    11881 aacaacgcct cgcggtatt
//
LOCUS       DAJTYH010000109        11698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTYH010000109 DAJTYH010000000
VERSION     DAJTYH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO609_003201"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO609_003201"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO609_003202"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO609_003202"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6634517.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO609_003203"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO609_003203"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6634518.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO609_003204"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO609_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO609_003205"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO609_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6634519.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO609_003206"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO609_003206"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6634520.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO609_003207"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO609_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6634521.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO609_003208"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO609_003208"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6634522.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO609_003209"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO609_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6634523.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO609_003210"
     CDS             9553..10332
                     /locus_tag="OO609_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6634524.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO609_003211"
     CDS             10322..11131
                     /locus_tag="OO609_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6634525.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO609_003212"
     CDS             11218..11652
                     /locus_tag="OO609_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6634526.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
BASE COUNT         2799 a         3150 c         2966 g         2783 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggtt
//
LOCUS       DAJTYH010000110        11672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTYH010000110 DAJTYH010000000
VERSION     DAJTYH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..336
                     /locus_tag="OO609_003213"
     CDS             <1..336
                     /locus_tag="OO609_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6634527.1"
                     /translation="MCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQQQL
                     RKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQNDLL
                     LRATGGVCV"
     gene            complement(453..728)
                     /gene="yjfN"
                     /locus_tag="OO609_003214"
     CDS             complement(453..728)
                     /gene="yjfN"
                     /locus_tag="OO609_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6634528.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(877..1206)
                     /gene="bsmA"
                     /locus_tag="OO609_003215"
     CDS             complement(877..1206)
                     /gene="bsmA"
                     /locus_tag="OO609_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6634529.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1388..2137
                     /gene="yjfP"
                     /locus_tag="OO609_003216"
     CDS             1388..2137
                     /gene="yjfP"
                     /locus_tag="OO609_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6634530.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2134..2889)
                     /gene="ulaR"
                     /locus_tag="OO609_003217"
     CDS             complement(2134..2889)
                     /gene="ulaR"
                     /locus_tag="OO609_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6634531.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2997..4061)
                     /gene="ulaG"
                     /locus_tag="OO609_003218"
     CDS             complement(2997..4061)
                     /gene="ulaG"
                     /locus_tag="OO609_003218"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6634532.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4416..5813
                     /gene="ulaA"
                     /locus_tag="OO609_003219"
     CDS             4416..5813
                     /gene="ulaA"
                     /locus_tag="OO609_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6634533.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5829..6134
                     /gene="ulaB"
                     /locus_tag="OO609_003220"
     CDS             5829..6134
                     /gene="ulaB"
                     /locus_tag="OO609_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6634534.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6144..6608
                     /gene="ulaC"
                     /locus_tag="OO609_003221"
     CDS             6144..6608
                     /gene="ulaC"
                     /locus_tag="OO609_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6634535.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6622..7272
                     /gene="ulaD"
                     /locus_tag="OO609_003222"
     CDS             6622..7272
                     /gene="ulaD"
                     /locus_tag="OO609_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6634536.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7282..8136
                     /gene="ulaE"
                     /locus_tag="OO609_003223"
     CDS             7282..8136
                     /gene="ulaE"
                     /locus_tag="OO609_003223"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6634537.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8136..8822
                     /gene="ulaF"
                     /locus_tag="OO609_003224"
     CDS             8136..8822
                     /gene="ulaF"
                     /locus_tag="OO609_003224"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6634538.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8951..9226)
                     /locus_tag="OO609_003225"
     CDS             complement(8951..9226)
                     /locus_tag="OO609_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6634539.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9553..9948
                     /gene="rpsF"
                     /locus_tag="OO609_003226"
     CDS             9553..9948
                     /gene="rpsF"
                     /locus_tag="OO609_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6634540.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9955..10269
                     /gene="priB"
                     /locus_tag="OO609_003227"
     CDS             9955..10269
                     /gene="priB"
                     /locus_tag="OO609_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6634541.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10274..10501
                     /gene="rpsR"
                     /locus_tag="OO609_003228"
     CDS             10274..10501
                     /gene="rpsR"
                     /locus_tag="OO609_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6634542.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10543..10992
                     /gene="rplI"
                     /locus_tag="OO609_003229"
     CDS             10543..10992
                     /gene="rplI"
                     /locus_tag="OO609_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6634543.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11063..11626)
                     /locus_tag="OO609_003230"
                     /pseudo
     CDS             complement(11063..11626)
                     /locus_tag="OO609_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2764 a         2869 c         3085 g         2954 t
ORIGIN      
        1 atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga cttattgtcg
       61 gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt tcgtcgttta
      121 cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca tcagctattc
      181 ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc gcaggcgtgg
      241 tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat ccagaatgat
      301 ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga tgcgacgctg
      361 gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg ctcgcgccgc
      421 atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc ctgtacgcgc
      481 cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga cgcaccttgt
      541 tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga aatattgcta
      601 actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc gaattctgca
      661 gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga ggcaagtaat
      721 tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt ccatcgcggt
      781 aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc atagggacgc
      841 aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca aaatggcttg
      901 tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt aataatcagc
      961 ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag aaccccgaac
     1021 catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc gaatctcttg
     1081 cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc tacaggcact
     1141 taacatcaac accaataata aactggcaaa ccggtaaata acgctattac gtttcctgct
     1201 aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa ttgttacctg
     1261 gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa cattatgaac
     1321 taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag acaaaaagga
     1381 gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc ttcatgctta
     1441 tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg gctttacttc
     1501 atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt tgcgggtgat
     1561 catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc ggcggttaaa
     1621 tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac gtgcggcaat
     1681 agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt cgatgggcgc
     1741 gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg ccagcatgat
     1801 gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga tacctgaaac
     1861 ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt gggaagcgac
     1921 aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc tcgacgatga
     1981 cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga cgggacggga
     2041 taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc ctgaggcgtt
     2101 agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc tgaccccttg
     2161 cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca gcatatcgat
     2221 ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc tatcaaccag
     2281 taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg tcagcatatc
     2341 ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc agtgcccggc
     2401 atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt tgttgtactg
     2461 tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg ccagcggtag
     2521 ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca gaaacgcggt
     2581 ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg ccgctttagc
     2641 aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg tccagcgcgg
     2701 gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc cgctttcgcc
     2761 aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct caacgacttt
     2821 ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat gtctttgtgc
     2881 ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg aaatagccag
     2941 gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac gacagattac
     3001 aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa accgcgcgga
     3061 tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc aacctgccag
     3121 ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag cacgcggatc
     3181 tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat aaccactttc
     3241 gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc ggtaataccg
     3301 cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt accgtgcttc
     3361 gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc aggggttttg
     3421 aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc cgctttttga
     3481 tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg aatttcaatg
     3541 tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt cggtacgccc
     3601 cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc atcagcacag
     3661 ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc gtgagtcgcc
     3721 agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt ggtgcgtagg
     3781 tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg tttcattaac
     3841 gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac gttggtgcca
     3901 ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa tgtgccaggg
     3961 gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg gaaagtgctc
     4021 aggatccagg attctcgggt gatacttttc actttactca tcgccattta ccttcatgat
     4081 agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat tccttcagga
     4141 aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt gagtggggaa
     4201 agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata attgtttcgt
     4261 tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca tttaatcata
     4321 aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt ccgggtgtgc
     4381 ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac aacatcttta
     4441 ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt gtgacctgtc
     4501 tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg attaaaacca
     4561 taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc ttcaaaccgg
     4621 tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat acttacgcgt
     4681 cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt tacgcggtgt
     4741 tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc ggtattcgta
     4801 ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc gttacgttgt
     4861 ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg gtttcgctct
     4921 actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg gacggctgcg
     4981 gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa gttgcgccgt
     5041 tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg ctgaatattt
     5101 tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt gccattctgc
     5161 tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg acggtctata
     5221 tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag ggtgtgcgca
     5281 tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg atccctggtg
     5341 cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc gtggtctggg
     5401 gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc gcctgcggtt
     5461 cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc accatcggcg
     5521 tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg gtgatgggga
     5581 tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt gcctggatgg
     5641 ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc tccatcggta
     5701 tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc gctggccgcg
     5761 cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct taataaggag
     5821 ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc agttccatga
     5881 tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac acggtaaata
     5941 gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc atcgcttcta
     6001 cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc gtgcgcaaca
     6061 tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg catttcccgc
     6121 aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag aataaatcca
     6181 tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc gttgacctgc
     6241 tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat gcggttgaac
     6301 agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt cgcccggaag
     6361 agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg gagttcaacc
     6421 acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat gccaacacgc
     6481 accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa aatttcgacc
     6541 gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc accaacgcgg
     6601 cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct ggacaaccag
     6661 actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga cattatcgaa
     6721 gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct gaaagcgctc
     6781 tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa aattctttct
     6841 cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc ggatatcaac
     6901 accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca gatcgaactg
     6961 accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat tcagcaggtg
     7021 gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga agccgatatc
     7081 actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg tggcctggcg
     7141 ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc cggtcgcagc
     7201 atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc catcgccgaa
     7261 ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat ctatgaaaaa
     7321 gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac gttgggcttc
     7381 gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct tgactggagc
     7441 cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg tgtgccgtcc
     7501 atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc ggtgcgggcg
     7561 caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg cattcgcgtg
     7621 atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac gcgtcgtcgt
     7681 ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt gacgctggcg
     7741 atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg ttacgcgcac
     7801 tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc ggcgtgggac
     7861 aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt gcatgtgaaa
     7921 gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt ggatttcgaa
     7981 cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat tgagatgtgg
     8041 agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg ggtgaaagcg
     8101 cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa cagcaggtgt
     8161 ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg ggcaacgtca
     8221 gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt gcctacgaaa
     8281 ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg gaaggggagt
     8341 atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac ccgtcgcttg
     8401 gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg gggctggcga
     8461 tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg tgtacgcgtg
     8521 gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa gtgattatcg
     8581 aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat cagcacggac
     8641 cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg atggaagaag
     8701 tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat atcgacagct
     8761 acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac gggcagaagt
     8821 aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt ccgcagccgt
     8881 ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt ttcgttggtt
     8941 atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta ctccccggtt
     9001 cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct tcatttaatg
     9061 cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg cctaagcttt
     9121 gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc catgtatttg
     9181 cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat acctcctcga
     9241 cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta atcaatgata
     9301 aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt gacggaatgt
     9361 gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt atttctgctc
     9421 cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca gccgaccttt
     9481 aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga ataatccgta
     9541 aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga ccagagcgaa
     9601 caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga aggcaagatc
     9661 caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa actgcacaaa
     9721 gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga gctggaaact
     9781 accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa gcacgctgtt
     9841 accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg cgatgatttc
     9901 gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt tctgatgacc
     9961 aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt cagtccatca
    10021 ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga agccggcttt
    10081 caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa ccaggccatt
    10141 actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc atgccacaag
    10201 gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt gatagattct
    10261 ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt tcaccgcgga
    10321 aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca tcaccgaaag
    10381 cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc gtcagctggc
    10441 tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc gccatcagta
    10501 atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt tattctgctt
    10561 gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc gggctatgct
    10621 cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa cattgaattc
    10681 ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc agctgctaat
    10741 gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa agctggcgac
    10801 gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt aactgcagct
    10861 ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg taccactggc
    10921 gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat cgtaaacgta
    10981 gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg tcggcgtttt
    11041 gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca ttctcttgtg
    11101 tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt atgtctcggt
    11161 cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt gataaggaac
    11221 gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat gcagcaatga
    11281 tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata taaagatcga
    11341 ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt acaacgtatc
    11401 ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca acctcgtgta
    11461 ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat ttcagttctg
    11521 cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata ttatctaatg
    11581 tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa tcgccacggg
    11641 tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAJTYH010000111        11571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTYH010000111 DAJTYH010000000
VERSION     DAJTYH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..2342)
                     /locus_tag="OO609_003231"
     CDS             complement(<1..2342)
                     /locus_tag="OO609_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6634544.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO609_003232"
     CDS             complement(2276..2899)
                     /locus_tag="OO609_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6634545.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO609_003233"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO609_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO609_003234"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO609_003234"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6634546.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEYIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO609_003235"
     CDS             7507..8229
                     /locus_tag="OO609_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6634547.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11571)
                     /gene="yfaL"
                     /locus_tag="OO609_003236"
                     /pseudo
     CDS             complement(8357..11571)
                     /gene="yfaL"
                     /locus_tag="OO609_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2658 a         3225 c         2873 g         2815 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgta ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg ttataaaccg ccaagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca t
//
LOCUS       DAJTYH010000112        11565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTYH010000112 DAJTYH010000000
VERSION     DAJTYH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..846
                     /locus_tag="OO609_003237"
     CDS             <1..846
                     /locus_tag="OO609_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6634548.1"
                     /translation="NTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALES
                     RLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQG
                     ENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYD
                     VDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKE
                     IEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKT
                     APQAK"
     gene            complement(1049..1402)
                     /locus_tag="OO609_003238"
     CDS             complement(1049..1402)
                     /locus_tag="OO609_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6634549.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1540..3186)
                     /gene="groL"
                     /locus_tag="OO609_003239"
     CDS             complement(1540..3186)
                     /gene="groL"
                     /locus_tag="OO609_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6634550.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3230..3523)
                     /locus_tag="OO609_003240"
     CDS             complement(3230..3523)
                     /locus_tag="OO609_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6634551.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3799..5055
                     /gene="yjeH"
                     /locus_tag="OO609_003241"
     CDS             3799..5055
                     /gene="yjeH"
                     /locus_tag="OO609_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6634552.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5071..5547)
                     /gene="fxsA"
                     /locus_tag="OO609_003242"
     CDS             complement(5071..5547)
                     /gene="fxsA"
                     /locus_tag="OO609_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6634553.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5884..7320
                     /gene="aspA"
                     /locus_tag="OO609_003243"
     CDS             5884..7320
                     /gene="aspA"
                     /locus_tag="OO609_003243"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6634554.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7438..8739
                     /gene="dcuA"
                     /locus_tag="OO609_003244"
     CDS             7438..8739
                     /gene="dcuA"
                     /locus_tag="OO609_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6634555.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8855..9193
                     /gene="cutA"
                     /locus_tag="OO609_003245"
     CDS             8855..9193
                     /gene="cutA"
                     /locus_tag="OO609_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6634556.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9169..10866
                     /gene="dsbD"
                     /locus_tag="OO609_003246"
     CDS             9169..10866
                     /gene="dsbD"
                     /locus_tag="OO609_003246"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6634557.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10903..11478
                     /locus_tag="OO609_003247"
     CDS             10903..11478
                     /locus_tag="OO609_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6634558.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2765 a         3003 c         2855 g         2942 t
ORIGIN      
        1 aataccggtg ttattcgcaa gagtaataat ttcattgcac tggcgctgaa aattaaagaa
       61 cctcgcaata aagaatcgtt gttctttatg tctgtcatgg aactgcgcga cctgctgatt
      121 gcgctggaaa gtcgtctgca tcaaaagcat aaactggatg ctgctgcacg ccttcagtat
      181 gagcaagctc gcgataaagt cattaagaaa atggcagaaa acatcccaga aattctggtt
      241 gatgagctta aaaatgccga tattaatcgt cgggttaaca cgctggaact taccgataat
      301 caaggcgaaa atcttacctt cgtattaacg ctacatgacg gtagcaaatg tgagttagtc
      361 gtcaatgagt tgcagattga aatgctggcg cgagcaatca ttcatgcaat taataatgcg
      421 gaaatgcgtg aactggcatt acgtattacc tcactgttag atttcctgcc gttatacgac
      481 gttgactgcc aggacaatgg caatctggaa tatgacacct attcccaacc ggaatggaaa
      541 cataacttgt tcgaccatta tctggcggtg ctttaccgct ttaaagatga aagtggcaaa
      601 gaacaattta gcggcgcagt ggtgaaaacg cgtgaagcaa cgccgggtaa agaaatagag
      661 gcgatcactc gccgaatgct tgatttcagc ccacgactga aaaaactcgc gggtgtgcca
      721 tgtcaggtct atgttcgcac cgtagcggcg aataacgccc aaccgctaac ccaggatcaa
      781 tgcttacgcg cgctgcatca cttacgtgta caatccacca gcaaaactgc accacaggcg
      841 aaataatctc atctttgcta atgcgaggct ggagccatcc agcctgaaat gactgataac
      901 gtgatcgttg gtcatgccgg atgcggcgta aacgccttat ccggcctaca aattctcaag
      961 cattcaatca gttgcaatgc ctgctgtagg cctgataagc gaagcgcatc aggcaataat
     1021 acgtttgtca cccagcctct ggatcacatc agttcgggca cttataaacc tgaccgataa
     1081 tctggctatc cgtcgggaca aaactggaca acattccctg cgacgggctg ctgatgccat
     1141 aaatcacgtt accgcccatt gcagccgcct ggttgcgcag atcgtttgct gcgccgcgca
     1201 tagaaccgcc ctcttctccg tgttgcccgg aaagccagtt gctttgctta cctgtcgcag
     1261 taccaatcag ctggcactct gcgcctggct gctcgtccac aatgcgtaca ctctgaccgg
     1321 cagcactcaa ttcgttgctg gagctacaac ccgccatcag tagcgcggca ccgacaatcc
     1381 ctgctaagta ttttacgtgc atgttattcc ccataatcaa tgagctggac gccactcgcg
     1441 tcatccgtgt ctgaatctta tactagaaag atgaccaaaa gaaaaacccc cagacatttc
     1501 tgcccggggg tttgtttatt tctgcgaagt gcagggcaat tacatcatgc cgcccatgcc
     1561 acccatgccg cccataccgc cagcagcgcc taagtcagct gcatcgtttt tcggcaggtc
     1621 ggtaaccatg cattcggtgg tgatcatcag gccagccaca gaagctgcgt actgcagagc
     1681 agaacgggtt actttggttg ggtccaggat acccatgtcg atcatgttgc cgtattcttc
     1741 ggttgctgcg ttgtaaccgt agttgccgtc gccgccttta acggtgttag caacaacaga
     1801 cggttcttcg ccgcagttca ggacgatctg acgcagcgga gcttccattg cacgcagtgc
     1861 aactttgata cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca gtttagacgc
     1921 tacgcggatc agcgcaacac caccaccagc aaccacgcct tcttctaccg cagcacgggt
     1981 cgcgtgcagg gcatcttcaa cgcgtgcttt tttctctttc atttcaactt cggtagcagc
     2041 acctactttg ataactgcaa cgccgcctgc cagtttcgct acgcgctcct gcagtttttc
     2101 acggtcgtag tcagaagttg cttcttcaat ctgctgacgg atctgagcaa cacggccctg
     2161 gattgcagct tcttcaccca cgccatcgat gatagtggtg gtgtctttgt tgatcacaac
     2221 acgtttagcc tgacccaggt cttccagggt tgctttttcc agctccatac cgatctcttc
     2281 agagattacg gtaccgccag tcagggttgc gatatcctgc agcatagctt tacgacgatc
     2341 gccgaagccc ggagctttaa ccgcagcaac tttcacgatg ccacgcatgg tgttaacaac
     2401 cagagttgct agcgcttcgc cttcaacatc ttcagcgatg atcagcagcg gtttgcctgc
     2461 tttggcaaca gcttccagaa ccggcagcat ttcgcggatg ttggagattt tcttgtcagc
     2521 cagcaggatg aacgggcttt ccagttctac tgcgccagtt tccggcttgt tgatgaagta
     2581 aggagacagg tagccacggt cgaactgcat accttcaacc acgtccagtt cgtcctgcag
     2641 accggtaccg tcttcaacgg tgataacgcc ttctttaccg actttgtcca tcgcttcagc
     2701 gatcagttta cctacggttt cgtcggagtt agcggagatg gtaccaacct gagcaatcgc
     2761 tttagaatca gagcacggta cggacagcgc tttcagttct tcaactgcag cggtaaccgc
     2821 tttgtcgata ccacgtttca ggtccatcgg gttcatgccc gcagcaacag ctttcagacc
     2881 ttcagtgatg atagcctgag ccagtacggt tgcagtggtg gtaccgtcgc ctgcagcgtc
     2941 gttcgctttg gaggcaactt ctttcaccat ctgcgcaccc atattttcga acttgtcttc
     3001 cagttcgatt tcacgagcaa cggaaacacc atctttggtg atggtcggtg caccgaaaga
     3061 tttatccaga actacgttac ggccttttgg accgagggta actttcactg catctgccag
     3121 tacgtttacg ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta cgtctttagc
     3181 tgccattatc tttattcctt aaattcgtat gttcagtgtc gtgcgcggat tacgcttcaa
     3241 caattgccag aatgtcgctt tcggacatga tcaacacttc ttcattgtcg atcttctcgg
     3301 atttcacacc gtagccatcg ttgaaaataa cgatgtcgcc aactttcacg tccagcggct
     3361 tcacttcgcc attttcaagg atacggccat tgccgacagc cagcacttcg ccgcgggtgg
     3421 atttagccgc tgcagagccg gtcagaacga tgccgccagc agatttagtt tcaacttctt
     3481 tacgcttgac gatcacgcga tcatgcaatg gacgaatatt cattgataac tctcctttga
     3541 gaaagtccgt atctgttatg ggtgacgccg gagcttacgt ggtttcccgg ctggtgacca
     3601 gagaaatggg gatgaggctt cgccccttca agggggaaaa agaaaaaaaa ttctgatcag
     3661 cacaaaatcg ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc ttcatcgccg
     3721 tcataagcga atctgattgt gctaccatcg aaaatctacg cagttgccca aaatttgggc
     3781 gcaatcggat atcaacccat gagtggactc aaacaagaac tggggctggc ccagggcatt
     3841 ggcctgctat cgacgtcatt attaggcact ggcgtgtttg ccgttcctgc gttagctgca
     3901 ctggtagcag gcaataacag cctgtgggcg tggcccgttt tgattatctt agtgttcccg
     3961 attgcgattg tgtttgcgat tctgggtcgc cactatccca gcgcaggcgg cgtcgcgcac
     4021 ttcgtcggta tggcgtttgg ttcgcggctt gagcgagtca ccggctggct gtttttatcg
     4081 gtcattcccg tgggtttgcc tgccgcacta caaattgccg ccggattcgg ccaagcaatg
     4141 tttggctggc atagctggca actgttgttg gcagaactcg gtacgctggc gttggtgtgg
     4201 tatatcggta ctctcggtgc cagttccagt gctaatctac aaaccgttat tgccggactt
     4261 atcgtcgcac tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc gagtatcccc
     4321 ttccccgcgc caggaaatat cgaacttacc gggttatttg ctgcgttatc agtgatgttc
     4381 tggtgttttg tcggtctgga agcatttgcc catcttgcct cggaatttaa aaatccagag
     4441 cgtgattttc ctcgtgcttt gatgattggt ctgctgctgg caggattagt ctactggggc
     4501 tgtacggtag tcgtcttaca cttcgacgcc tatggtgaac aaatggcggc ggcagcatcg
     4561 cttccaaaaa ttgtagtaca gttgttcggt gtaggagcgt tatggattgc ctgcgtaatt
     4621 ggctatctgg cctgctttgc cagtcttaac atttatatac agagcttcgc ccgcctggtc
     4681 tggtcgcagg cgcaacataa tcctgaccac tacctggcac gcctctcttc tcgccatatc
     4741 ccgaataatg ccctcaatgc ggtgcttggc tgctgcgtgg tgagcacgtt ggtaattcat
     4801 gccttagaga tcaatctgga cgctcttatt atttatgcca atggcatctt tattatgatt
     4861 tatctgttat gcatgctggc aggctgtaaa ttattgcaag gtcgttatcg actactggcg
     4921 gtggttggcg ggctgttatg cgttctgtta ctggcaatgg tcggctggaa aagtctctat
     4981 gcgctgatca tgctggtggg gttatggctg tttctgccaa aacgaaaaac gccggaaaat
     5041 ggcataacca cataatccgg cgtttcgaca ttaatcctgg cgatcgtctt tatgatcaag
     5101 gcggtcgcgg tcatcatcct ttcgctgata ctcaccatca aaagtattac cgccaccggt
     5161 cccggcgcta aaaccgccgc ctggcatgcg agaaaagcgc aaatgtggca tcaacttcac
     5221 tgtcagatgc ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt cggtgaaaaa
     5281 gcccggtaat aaaagcagca aaccggcaat aatcagcgaa acacttttaa tcatctccgc
     5341 cgcagggttt tcacccgccg ccattttttg ctgcatcagc acgaaattct taaagccctg
     5401 gttacgtacc agtgacatac cgataactga cgtgaatata accagcacga gggtcagcaa
     5461 tacccccaat acatgggcaa cctgaataaa gattgaaatc tcaatataga cataaaggaa
     5521 aatggcaata aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc ggttaaaccc
     5581 ggctacctga atgggttgcg aatcgccttc agcttatatt gtggtcatta gcaaaatttc
     5641 aagatgtttg cgcaactatt tttggtagta atcccaaagc ggtgatctat ttcacaaatt
     5701 aataattaag gggtaaaaac cgacacttaa agtgatccag attacggtag aaatcctcaa
     5761 gcagcatatg atctcgggta ttcggtcgat gcaggggata atcgtcggtc gaaaaacatt
     5821 cgaaaccaca tatattctgt gtgtttaaag caaatcattg gcagcttgaa aaagaaggtt
     5881 cacatgtcaa acaacattcg tatcgaagaa gatctgttgg gtaccaggga agttccagct
     5941 gatgcctact atggtgttca cactctgaga gcgattgaaa acttctatat cagcaacaac
     6001 aaaatcagtg atattcctga atttgttcgc ggtatggtaa tggttaaaaa agccgcagct
     6061 atggcaaaca aagagctgca aaccattcct aaaagtgtag cgaatgccat cattgccgca
     6121 tgtgatgaag tcctgaacaa cggaaaatgc atggatcagt tcccggtaga cgtctaccag
     6181 ggcggcgcag gtacttccgt aaacatgaac accaacgaag tgctggccaa tatcggtctg
     6241 gaactgatgg gtcaccagaa aggtgaatat cagtacctga acccgaacga ccatgttaac
     6301 aaatgtcagt ccactaacga cgcctacccg accggtttcc gtatcgcagt ttactcttcc
     6361 ctgattaagc tggtagatgc gattaaccaa ctgcgtgaag gctttgaacg taaagctgtc
     6421 gaattccagg acatcctgaa aatgggtcgt acccagctgc aggacgcagt accgatgacc
     6481 ctcggtcagg aattccgcgc tttcagcatc ctgctgaaag aagaagtgaa aaacatccaa
     6541 cgtaccgctg aactgctgct ggaagttaac cttggcgcaa cagcaatcgg tactggtctg
     6601 aacacgccga aagagtactc tccgctggca gtgaaaaaac tggctgaagt cactggcttc
     6661 ccatgcgtac cggctgaaga cctgatcgaa gcgacctctg actgcggcgc ttatgttatg
     6721 gttcacggcg cgctgaaacg cctggctgtg aagatgtcca aaatctgtaa cgacctgcgc
     6781 ttgctctctt ctggtccacg tgccggcctg aacgagatca acctgccgga actgcaggcg
     6841 ggctcttcca tcatgccagc taaagtaaac ccggttgttc cggaagtggt taaccaggta
     6901 tgcttcaaag tcatcggtaa cgacaccact gttaccatgg cagcagaagc aggtcagctg
     6961 cagttgaacg ttatggaacc ggtcattggc caggccatgt tcgaatccgt tcacattctg
     7021 accaacgctt gctacaacct gctggaaaaa tgcattaacg gcatcactgc taacaaagaa
     7081 gtgtgcgaag gttacgttta caactctatc ggtatcgtta cttacctgaa cccgttcatc
     7141 ggtcaccaca acggtgacat cgtgggtaaa atctgtgccg aaaccggtaa gagtgtacgt
     7201 gaagtcgttc tggaacgcgg tctgttgact gaagcggaac ttgacgatat tttctccgta
     7261 cagaatctga tgcacccggc ttacaaagca aaacgctata ctgatgaaag cgaacagtaa
     7321 tcgtacaggg tagtacaaat aaaaaaggca cgtcagatga cgtgcctttt ttcttgtgag
     7381 cagtaactta aaaataacaa tctaatatca acttgttaaa aaacaaggaa ggctaatatg
     7441 ctagttgtag agctcatcat agttttgctg gcgatcttct tgggcgccag attgggggga
     7501 ataggtattg gttttgcagg cggattgggg gtgctggttc ttgccgctat tggcgttaaa
     7561 cccggtaaca tcccgttcga tgtcatttcc attatcatgg cggttatcgc cgctatttct
     7621 gccatgcagg ttgctggcgg tctggactat ctggttcatc agacagaaaa gctgctgcgc
     7681 cgtaacccga aatacatcac gatcctcgca ccgatcgtga cctatttcct gactatcttt
     7741 gctggtactg gcaacatctc tctggcgaca ctgccagtta tcgctgaagt tgcgaaggaa
     7801 caaggcgtta aaccttgccg tccgctctct actgcagtgg tatccgcgca gattgcgatc
     7861 accgcatcgc caatctcagc ggcagtggtt tacatgtctt ccgtgatgga aggtcatggc
     7921 atcagctacc tccatctgct ctccgtggtc atcccgtcca ccctgctggc ggttctggtg
     7981 atgtccttcc tggtcactat gctgttcaac tccaaactct ctgacgatcc gatttatcgc
     8041 aagcgtctgg aagagggctt ggttgaactg cgcggtgaaa agcagattga aatcaaatcc
     8101 ggtgcaaaaa cgtccgtctg gctgttcctg ctgggcgtag ttggcgtggt tatctatgca
     8161 atcatcaaca gcccaagcat gggtctggtt gaaaaaccgc tgatgaacac caccaacgca
     8221 atcctgatca tcatgctcag cgttgcaact ctgaccaccg ttatctgtaa agtcgatacc
     8281 gacaacatcc tcaactccag caccttcaaa gcaggtatga gcgcctgtat ttgtatcctg
     8341 ggtgttgcgt ggctgggcga tactttcgtt tccaacaaca tcgactggat caaagatacc
     8401 gctggtgaag tgattcaggg tcatccgtgg ctgctggccg tcatcttctt ctttgcttct
     8461 gctctgctgt actctcaggc tgcaaccgca aaagcactga tgccgatggc tctggcactg
     8521 aacgtttcac cgctgaccgc tgttgcttct ttcgctgcgg tgtctggtct gttcattctg
     8581 ccgacctacc cgacgctggt tgctgcggta cagatggatg acacgggtac tacccgtatc
     8641 ggtaaattcg tcttcaacca tccgttcttc atcccgggta ctctgggtgt tgccctggcc
     8701 gtttgcttcg gcttcgtgct gggtagcttc atgctgtaat gatcaatcgc ggggcgttca
     8761 cgccccgctt tctttcccgc cgactaacat ccttcccccg tccgttgtat agtgacctct
     8821 ctcttgcggt tccatctgtt cttgcgaggt gtttatgctt gatgaaaaaa gttcgaatac
     8881 cacgtctgtc gtggtgctat gtaccgcacc ggatgaagcg acagcccagg atttagccgc
     8941 caaagtgctg gcggaaaaac tggcggcctg cgcgaccttg atccccggcg ctacctctct
     9001 ctattactgg gaaggtaagc tggagcaaga atacgaagtg cagatgattt taaaaactac
     9061 cgtatctcac cagcaggcac tgctggaatg cctgaagtct catcatccat atcaaacccc
     9121 ggaacttctg gttttacctg ttacacacgg agacacagat tacctctcat ggctcaacgc
     9181 atctttacgc tgattctgct actttgcagc acttccgttt ttgccggatt attcgacgcg
     9241 ccgggacgtt cacaatttgt ccccgcggat caagcctttg cttttgattt tcagcaaaac
     9301 caacatgacc ttaatctgac ctggcagatc aaagacggtt actacctcta ccgtaaacag
     9361 atccgcatta cgccggaaca cgcgaaaatt gccgacgagc agctgccgca aggcgtctgg
     9421 catgaagatg agttttacgg caaaagcgag atttaccgcg atcggctgac gcttcccgta
     9481 accatcaacc aggcgagtgc gggagcgacg ttaactgtca cctaccaggg ctgtgctgat
     9541 gccggtttct gttatccgcc agaaaccaaa accgttccgt taagcgaagt ggtcgccaac
     9601 aacgcagcgt cacagcctgt gtctgtttcg cagcaagagc agcacaccgc gcaattgccc
     9661 ttttccgcgc tctgggcgtt gttgatcggt attggtatcg cctttacgcc atgcgtgctg
     9721 ccaatgtacc cactgatttc tggcatcgtg ctgggtggta aacagcggct ctccactgcc
     9781 agagcattgt tgctgacctt tatttatgtg caggggatgg cactgactta cacggcgctg
     9841 ggtctggtag ttgccgccgc agggttacag ttccaggcgg cgctacagca cccatacgtg
     9901 ctcattggcc tcgccatcgt ctttaccttg ctggcgatgt caatgtttgg cttgtttact
     9961 ctacaactcc cctcttcgct gcaaacacgt ctcacgttga tgagcaatcg ccaacagggc
    10021 ggctcaccta gcggtgtgtt tgttatgggg gcgattgccg gactgatctg ttcaccatgc
    10081 accaccgcac cgcttagcgc gattctgctg tatatcgccc aaagcgggaa catgtggccg
    10141 ggcggcggca cgctttatct ctatgcgttg ggcatgggcc tgccgctgat gctaattacc
    10201 gtctttggta accgcttgct gccgaaaagc ggcccgtgga tggaacaagt caaaaccgcg
    10261 tttggttttg tgatcctcgc actgccggtc ttcctgctgg agcgagtgat tggtgatgta
    10321 tggggattac gcttgtggtc ggcgctgggt gtcgcattct ttggctgggc ctttatcacc
    10381 agcctacagg ctaaacgcgg ctggatgcgc gtggtgcaaa taatcctgct ggcagcggca
    10441 ttggttagcg tgcgcccact tcaggattgg gcatttggtg cgacgcatac cgcgcaaact
    10501 caaacgcatc tcaactttac acaaatcaaa acggtagatg agttaaatca ggcgctcgtt
    10561 gaagccaaag gcaaaccggt gatgttagat ctttatgccg actggtgcgt cgcctgtaaa
    10621 gagtttgaga aatacacctt cagcgacccg caggtgcaaa aagcgttagc agacacggtc
    10681 ttacttcagg ccaacgtcac ggccaacgac gcacaagatg tggcgctgtt aaagcatctt
    10741 aatgtccttg gcctaccgac aattctcttt tttgacggac aaggccagga gcatccacaa
    10801 gcacgcgtca cgggctttat ggatgctgaa accttcagcg cacatttgcg cgatcgccaa
    10861 ccgtgaacga cacttgcagt gggaaagacg gaggaaaata gcgtgcaacg tgaagatgta
    10921 ctgggagaag ccctgaaatt attagaatta caagggattg ccaacaccac gctggagatg
    10981 gttgctgaac gtgtggatta tccactggac gagctacgcc gcttctggcc agacaaagag
    11041 gcaatcctct acgatgcgct gcgttatctt agccaacaga tagatgtctg gcgtcgtcag
    11101 ctgatgctgg acgaaacgca aaccgccgaa caaaagctgc tggcacgtta tcaggcgtta
    11161 tcggagtgcg ttaaaaacaa ccgctatccg ggctgtctat ttatcgctgc ctgtacgttt
    11221 tatcccgatc ctggccaccc tattcatcaa ctggccgatc agcaaaaaag cgctgcctac
    11281 gatttcaccc acgaactgtt aaccacgctg gaagttgacg atccggcgat ggtagcaaag
    11341 cagatggaac tggtgctgga aggctgttta agccgaatgc tggtgaatcg tagccaggcg
    11401 gatgtcgaca ctgcacatcg gctggcggaa gatatcctgc gcttcgcccg ctgccgccag
    11461 ggtggtgcat tgacctgaca gaaacacaga aaagaagcga tttgccgcaa tcttaagcag
    11521 ttgaatcgct tttactgaaa ttaggttgac gagatgtgca gatta
//
LOCUS       DAJTYH010000113        11550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTYH010000113 DAJTYH010000000
VERSION     DAJTYH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..583)
                     /gene="cheZ"
                     /locus_tag="OO609_003248"
     CDS             complement(<1..583)
                     /gene="cheZ"
                     /locus_tag="OO609_003248"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6634559.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTS"
     gene            complement(594..983)
                     /gene="cheY"
                     /locus_tag="OO609_003249"
     CDS             complement(594..983)
                     /gene="cheY"
                     /locus_tag="OO609_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6634560.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(998..2047)
                     /gene="cheB"
                     /locus_tag="OO609_003250"
     CDS             complement(998..2047)
                     /gene="cheB"
                     /locus_tag="OO609_003250"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6634561.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2050..2910)
                     /gene="cheR"
                     /locus_tag="OO609_003251"
     CDS             complement(2050..2910)
                     /gene="cheR"
                     /locus_tag="OO609_003251"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6634562.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(2929..4530)
                     /gene="tap"
                     /locus_tag="OO609_003252"
     CDS             complement(2929..4530)
                     /gene="tap"
                     /locus_tag="OO609_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6634563.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4576..6237)
                     /gene="tar"
                     /locus_tag="OO609_003253"
     CDS             complement(4576..6237)
                     /gene="tar"
                     /locus_tag="OO609_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6634564.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6382..6885)
                     /gene="cheW"
                     /locus_tag="OO609_003254"
     CDS             complement(6382..6885)
                     /gene="cheW"
                     /locus_tag="OO609_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6634565.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(6906..8870)
                     /gene="cheA"
                     /locus_tag="OO609_003255"
     CDS             complement(6906..8870)
                     /gene="cheA"
                     /locus_tag="OO609_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6634566.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(8875..9801)
                     /gene="motB"
                     /locus_tag="OO609_003256"
     CDS             complement(8875..9801)
                     /gene="motB"
                     /locus_tag="OO609_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6634567.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9798..10685)
                     /gene="motA"
                     /locus_tag="OO609_003257"
     CDS             complement(9798..10685)
                     /gene="motA"
                     /locus_tag="OO609_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6634568.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10812..11390)
                     /gene="flhC"
                     /locus_tag="OO609_003258"
     CDS             complement(10812..11390)
                     /gene="flhC"
                     /locus_tag="OO609_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6634569.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11393..>11550)
                     /locus_tag="OO609_003259"
     CDS             complement(11393..>11550)
                     /locus_tag="OO609_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6634570.1"
                     /translation="HFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLNDVNQPEE
                     ALRKKRA"
BASE COUNT         2613 a         3243 c         2835 g         2859 t
ORIGIN      
        1 tgctggtatc gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact
       61 cctgttccgg gatgttttcc aacagcacca tcagcaactg gcgttcgatc tcctgaatga
      121 catccatcat ccgcttaata acctgcccgg tgagatcctg aaaatcctgc gccatcatga
      181 tttccagcag ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc
      241 gtgtatctgt taccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat
      301 cccaacgttg ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg
      361 cctcaacact gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca
      421 aacgatcgcg cgcatcgggg atggcttccg ccgcttcggc aatggcctga accagcccca
      481 gttcccgcaa actgtcgcgc agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc
      541 cagctgaatg ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg
      601 cccagtttct caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc
      661 accacatagc cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct
      721 gcagtcacca ttaacactgg caatgccgac atcgcgccat ccgcacgaat tgttttcagc
      781 aattccaggc catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct
      841 gcctgcaact tattgagagc gtcgacacca tcttccgctt cctcaacatt attgaatccc
      901 agctctttca gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa
      961 aatttaagtt ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg
     1021 cactaattgt tgccaacatt tgctggctta cctggctaag atcgaccact tcgcagacac
     1081 cacccatatt gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg
     1141 caagggtcca tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca
     1201 taccggtcag gatcacccca accgcattac gccccgcctg tttggcgaca gaatggaaca
     1261 acacatctac cgaaggccga tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat
     1321 aatttgcgcc actacgcgcc agttccatat gccgatcgcc cggcgcaata taggcatgcc
     1381 ccggcaagac acgttctccg tcttcggctt ctttaacccc gatctggcaa agcttattaa
     1441 gtctgtcggc aaaagagcgg gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg
     1501 ggctggaaag cggcaacggt tgcagtacgt gacgaattgc ctcagttcca cccgttgaag
     1561 caccaatcgc aatcagtttt tcagaactca acaacggccc tgccttcagc gttgtcggtg
     1621 ctgacaatgg cttatgtgct gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa
     1681 tcatttcgct atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat
     1741 ctattgcccc cagctccagc gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag
     1801 aaaccatcac aacgggcatt ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt
     1861 ccatccgcgg catttcaaca tccagcgtca gcacatcggg attgaatttc ttaatcaagt
     1921 cacgcgcaac cagcggatca ggcgcggtcg ccaccatttc catgtcgcta tggctgttga
     1981 tgatttctgt catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt
     2041 tgctcatcgt taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg
     2101 ctcgaggtgg ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag
     2161 cggaacaaag cggcgcaaaa tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt
     2221 acgacagaag atcgcatcaa acggccccgg cacggtgtac tgtttcgcca gtaaattcag
     2281 cggggcaaaa tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg
     2341 tcccgtccct cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc
     2401 atggcgatag ataccgcttc tggctttttc cagcacttcg gtgtcgatat cactggcaaa
     2461 cactttccag cgtccgggca cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta
     2521 cggctcttcg ccggtcgaag ccgccgcgct ccatacgcga tcctcgccag aacggcgacg
     2581 tgcgtgatcc gcgagcagag ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt
     2641 ggtcagcgaa ttgataaacg cctgccactc accgctgtgc tgattagatt ccagcaagtt
     2701 cagataatga ccgaaatccg tcagtcccag agaacgcaaa cgacgaacca gtcggttgta
     2761 aaccatgtcg cgtttatggt cagccagaac gatcccggct cgttgataga tcaattgact
     2821 tatccgccga aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga
     2881 cgtttgccca cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac
     2941 tggcgcaatt tgtaactgcg ccgactcatg tcgtgctact tcatgttctt caagggtaaa
     3001 taccgccaca cgcgacgaaa gatggtcggc ctgattcgcc agttgttccg ttgccaccgc
     3061 cgcttcttct accagcgagg agttctgctg agtcacctga tccatctggc tgacagcctg
     3121 tgcaacctgc tcaatccccc gccgttgttc ttccgacgcc gaagcaattt ctcccataat
     3181 gtcgttcacg cgggtcaccg aactgacaat atcggtcatg gtcgcggcgg cgttattcac
     3241 cagtttcgaa ccctgctgga cacgattgac tgactcttcg atcagccctt tgatctcttt
     3301 tgctgcctgc gcgctacggc tggcaagatt gcgcacttca cctgccacta ccgcaaaacc
     3361 acgcccctgc tctccggcgc gagccgcttc cactgccgca ttcagggcca gaatattggt
     3421 ctggaaagca attccgtcga taacgctgat aatgtcgcca attttttgcg agctggtggc
     3481 gatctcctgc atggtgtgag tcatggtact gacctgaaca ccgcccgcct gcgccgttgt
     3541 cgcggcattt tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt
     3601 ggcggttaat tgctccatac tggcggccgt ttgtgccagc gatgccgcct gctgttcggt
     3661 acgacttgag agatcgttat tgcctgcgac aatctccgcg atcccaatgt gcatctcatg
     3721 gcttccctta cgcacatcac tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct
     3781 ggcaaaaatg gcggtgatct cattacgacc atataccgca atcggacgcg ccagattacc
     3841 cgcagcaatg ctgtcaaaat ggctaccgat aatggccagt ggttgaacaa tcatcttgcg
     3901 cgtccaccac agcgcactgc tgatgtagat cgccgcaaca ataatcatgc tgataaacac
     3961 cagcgccgaa atctgatagt tacgctggct ttgcgcactg gcggcttcca gcacatggtt
     4021 gatctccagc tgccaggcct caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg
     4081 cgcagtgagg aaatccgaaa gttgattact ttcaagccag gtcgcctgat gttcgagatc
     4141 gttgtgccag cgggcaaaac ttttctccgt ctctttttgc aatgccctga cgtgctcgtt
     4201 gcccgcagtc atcgccataa aacttttaaa cagcgtggtg gattgcgtca gactggcgcg
     4261 cgccgtcgtc atcaacgttt taatgtcatc cgccggatag ctaagcgctg tcagagtgcc
     4321 tgctttgttc agcgcggtac tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc
     4381 acgttgctga ttgctctgct cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca
     4441 aaaagacatg ccgttactgc caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag
     4501 cgtggtcgaa attcgaatac gattaaacat caacgctccc catcaggcgg caatgaccgc
     4561 gttagtaaaa actcgtcaaa atgtttccca gtttggatct tgttcagtaa ttcgcagtcg
     4621 tggctgtgcc ggtggttgct cactggcagg acgggatggt gtttgcggtt tattggtgag
     4681 tgggctggct gccagacgga acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc
     4741 cagcgcagct gcggcggcag ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg
     4801 atccatttcc gaaaccgcca atgcgacttg atcgatgcca cggctctgtt catccgatgc
     4861 cgatgcaatc tcgcccataa tgtcagtcac gcgagtgaca gcattgacga tattgttcat
     4921 tgtttccccg gcgctttcga ccagcaccga accggtatca atgcgcgaga cggagtcttc
     4981 aatgagggct ttgatctctt ttgccgccag ggcgctgcga ctggcaagat tacgcacttc
     5041 acccgccacc acggcaaaac cacggccctg ttcacccgca cgcgcggctt caaccgcggc
     5101 attcagcgcg aggatattag tctggaaggc aataccgtcg ataacgctga taatgtcggc
     5161 aattttcttc gaactatcgg cgatctcatg catcgttttc actacgccat ccaccacttt
     5221 gccgccgtgc tgggcggtgt cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt
     5281 atcggcgttt tgcttcactg tcgcggtgag ctgctccatg ctggcggcag tttcttccag
     5341 cgcggatgcc tgctgttcag tacgggagga aagatcggtg ttgcccgccg caatttcacg
     5401 ggtaccggca tagatggcat ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga
     5461 gcgttgcata tgtgaaacgc tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt
     5521 cagggtattc gccaggttac caccggcgat ttcgcaaatg tgagcaataa tttttgccag
     5581 cggagtaagc aacatacggc gaatgccgta ccacgccacc agcagaatca ataccaccac
     5641 cagcgcgata accgccagtt gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac
     5701 gatatcgcga tacagttttt cactgctgag ggcgtactga gcaaacgctt cgcccattgc
     5761 attttgcatt ccctgggttg gctgagcgaa ataagctcca gtattgccat aatcaagata
     5821 atcaatcagt tcagttaacg ctgtgtaata gtttttatat ttttcatcaa aattacggct
     5881 ggtagcgacc atttcaggta acggtgccat gcttttgaat tttttataat gcgtcgcggc
     5941 ctgggccaat gttttcctgg cgctatcgag caattcaact ttggcgttac tttgttgatt
     6001 ggaggaatcc atcatcatcc gcaccgctga acgactcagg ttaatgcgcg tttgcagcat
     6061 taaatcccag gttgacgtca gctcgccctg ctgttcccgt aattgattgg aaaccacaaa
     6121 gctcttctgg ctatggtgaa gggaagaaaa aaacaaactg ccggaaataa gctgtaacag
     6181 tgcgaatacc cccagcacca ttaccaacag cgtgactacg cggatacggt taatcataag
     6241 gcaccttcct gaaaacaagt tgatctcgtt atcggcaaga aggggggaaa ctttattgct
     6301 gatgccaccc gccgcgaaat tgaaataaaa aacccgatgc gcagatcatc gggttcattt
     6361 caattgagga aatcgggaga attacgccac ttctgacgcc gcgctatcta acagcgccat
     6421 ctcttcgctg ttcagcagtt tttcgatgtt caccagaatc aacatacggt cgcccagtgc
     6481 gcccagtcca gtgagatatt ctgttgaaag cgtcacggca aactctggtg ccggacgaat
     6541 ttgctccgcc gtcaatgaaa gcacgtctga aacgccgtca accacgatgc cgaccacccg
     6601 ctgtccgaga ttcaggacga taactaccgt gttgtcgtta tagtccacat ccacctggct
     6661 gaacttaatt cgtaagtcaa caatcggcac aataacgccg cgcagattcg tgacgccttt
     6721 gataaacgct ggcgtgttcg caatccgtgt tacctgatcg tagccacgga tctcctgcac
     6781 tttcaggata tcaataccgt actcttcatc accaagggta aataccagaa attcctggcc
     6841 tgacggctcg ctggccagct ttgttacatt cgtcataccg gtcatattgt taccttttta
     6901 ctcattcagg cggcggtgtt cgccatacgt tgttcgcggt ttatcgcctg caaggcggag
     6961 acatcaacaa tcagtgccac gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg
     7021 actttgcgat agttactttc aaggttttta accacaacct ggtgttgacc aattaattga
     7081 tccaccagca aggcgtagcg gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg
     7141 gcttcggttt tcgcgcccgc gacgttgaac actttccaca gttcgacgat gggcagatat
     7201 tcaccccgca cttccagcac ccgctcgccg ccggccagtg gatggagatc ggcttcacgg
     7261 ggttgcagtg attccataac agcattcagc ggcagaatga aaacttcatc cgcaacgcgt
     7321 acggacatgc cgtcgaggat ggccagcgtc agcggcagta aaatgcggat cgtagtgcca
     7381 gtaccctgct tcgactggat ttcgacatga ccgcccatct cctggatatt acgtttaacg
     7441 acgtccatgc cgacgccgcg cccggagacg tcggtgacct gctctgccgt ggagaagcct
     7501 ggtgcaaata tcagcattgc aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct
     7561 tgcgaggccg cttttgccag aattcgctca cggtttagcc ccgccccatc gtcggtcact
     7621 tcaatgcaaa tgttgccgcc ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt
     7681 ttacctgcgg cgagccgttt ttctggcagt tcaataccgt gatcgaggct attgcgtacc
     7741 aggtgggtca gcgggtcgat aatgcgttct atcaggctct tgtcgagctc ggtggaactg
     7801 cccaccagcg tcagttctac ctgcttgccg agttttcccg ccagatcacg caccagccgg
     7861 ggatagcgac taaagacata ttccatcggc atcatgcgaa tcgacatcac tgattcctgc
     7921 aaatcacggg cgttacgttg taactgcccc atgctggtaa tcaaatcacc atgattaacc
     7981 gggtccagtt cgctggaacg ctgggcaagc atggactggg tgataaccag ctcgccgacg
     8041 aggttaatta attgatcaac cttttctacc gctacacgga tgctggtgga ttcactgctg
     8101 cgcgtcgttt tttcccgctc cacgcggccg gttggcgctt gttcggctgc cagtttaagc
     8161 acaggtgggg tagctatttt tggcgaaact tctactgttt caaaggtaat ctgatcggct
     8221 tcaatcacaa aacagagtac cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt
     8281 gccgagagag aatccgcccc tttcaccacg tcatttaacg ttgtcagatg ccccagctct
     8341 tcttccagca ggtcgacttc gccggccttc aggcgcgaaa ggataattcg tcgcggcaac
     8401 tgactgcgac tttgctcatc ttgcggttca cttttggcaa ccacacttaa tcgggtcact
     8461 gcggatggcg tttcgccttt cgcttctaat gccagttgac gcaaggcctg gcagatataa
     8521 tcgtagctgg cggcatccgg ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg
     8581 atgtccttcg tttccaaaaa cagattgata atgtcggtgt tgagttgcat ctcacctcgt
     8641 ctggcttcat cgagcaggtt ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca
     8701 aaagttcctg cccctccttt gatcgagtgg gcagcccgaa agatggcatt caattgttcg
     8761 gcatctggcg cttccggctg caaaaccagc agatgctgct ccatatcagc caacagttcg
     8821 tccgcttcat caaaaaatgt ctgataaaaa tcgcttatat cgatgctcac gctgtcacct
     8881 cggttcggct gatggcattg tgggaacact gacctgtggt gcaacctcag gtttttctag
     8941 ggcgcttact ggctcattct ggctttcggc gttttcatgc aaaatggcct gttcggcttg
     9001 tttgttcagt accaacaggc tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct
     9061 taagcgcatc gttgccgcca tgccgacgac acgtaacact ttgccgctat ccaaccctcc
     9121 gaccatcagt tcgcggcggg atgcattggc ccgatcggca gaaagctccc agttgctgta
     9181 acctttctca ccgctggcgt agggaaaatc atcggtatga cctgaaaggc taatacggtt
     9241 gggaataccg ttcagcacag gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac
     9301 atcggcactg ccggttctaa acatcgggcg attctggcta tcgatgatct gaatacgtag
     9361 accttcctgg accagatcga ttttgagatg gagacgtaac gcccgcagtt tcggatcgga
     9421 ctctatcaac tggtcgagat caccccgcaa tttccgcaat cgactttgct ccatgcgttt
     9481 tttcagctct tcgatgttcg gctgcttatt cacttccccc tggctttggg tgtaatcatc
     9541 accaccgccg ggaattgggc tttcactatt ggaaatgcga tcgccgcccg taaccgctgt
     9601 cgccagtgga gtccggaagt actccgcaat ctgaatcaac tcttttgggc tggagatgga
     9661 gatcagccac atcaccagaa aaaaggccat catcgcagtc ataaagtcgg cataagcaat
     9721 cttccacgat ccatgtgctg ccccgtggct tttggctttg cgtcgtttga cgacaataat
     9781 cggatgcgct tgattcttca tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc
     9841 gcacgcacat gctcttccag ttcaataaac gacggacgtt cgctggaata gagcgttttg
     9901 cgaccaaact caacggcgat aggcggtgcg taaccgttca gattagaaag cagagtgact
     9961 ttgacgcact gcatcatttt gctggtttcg gcgcttttct gacgtaaaac actcgctagt
    10021 ggggaaataa atccgtaagc caataaaata ccgaggaaag tccccaccat cgcatgtgcg
    10081 ataagcgccc ccagctcggc ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc
    10141 attacagccg cgacaatacc aaacgccgga agtgagtccc cgaccagcgc cagactgttc
    10201 gccgggactt ctgcctcgct ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt
    10261 tcgaaggtgt tcatgtgacc gctgataatc aggcgcagat aatcgacgat aaaatcaagc
    10321 atgacgctat ccgcgaggat gcgtgggtag ctggcgaaga tctcgctctc acggggattt
    10381 tcaatatcac gttccagcga aaacatcccc atctgccgcg atttcgccat caaccgataa
    10441 agcagagcca gcagatccat atacattgct ttggtgtatt tggagcgacg aaacagcaac
    10501 ggcagcgctt tcagcgtgcc tttaatcgct ttgccattat tgccgacgat aaacgaccca
    10561 atccctgcac cggcaataat caccagttca gcgggttgat agagtgctcc aaggcttcca
    10621 ccggtcatca aataaccgcc gaaaactgta ccgagaacaa ccaggtaacc taataagata
    10681 agcacgacat catccttcca ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac
    10741 gcgcgattgg ggaaaaaaaa agcagcggta cgtcgttacc gctgctggaa tgttgcgcca
    10801 caccgtatca gttaaacagc ctgtactctc tgttcatcca gcagttgtgg gataatatcg
    10861 gcaggattct gggaaagttt acgtcttttt actgcccggg atggcggttg acataagctg
    10921 caggcaaagc tgccaacagg ctggtgagcg tgggtaataa aattgccgcc gcagcagttg
    10981 cagctggaaa gttgcagtaa tccactttca acaaaccgca ccaatgtcca ggcacgggtt
    11041 aatgccagca gtggtccttc ttctgcttgt gggcactgtt caaggtataa acggtaggct
    11101 ttgatcaccg catcgacgcc attacacaaa ccggttttca gtaaaaactg ccatgcatta
    11161 cagaacatcg aagcatgaac gttttgttcc caggtcataa accagtcggt tgagaatggc
    11221 agcatgcctt tcggcggtgg gcttccgcgc agttctttat aaagttttat caggcgtccg
    11281 cgacttaact gtgtttcgct ttccagcatc tgcaaacgag cgcccagggt gatcaattcc
    11341 attgccagct gaatatcccg cgcttcctga acaatgcttt tttcactcat gatcaggccc
    11401 ttttcttgcg cagcgcttct tcaggctgat taacatcatt cagcaagcgt gttgagagca
    11461 tgatgccggt atgaatttgc tggagatcgt caacgcggga atcttgcgtc aactgagtaa
    11521 tcgtctggtg gctgtcaaaa cggaagtgac
//
LOCUS       DAJTYH010000114        11511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTYH010000114 DAJTYH010000000
VERSION     DAJTYH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            523..720
                     /locus_tag="OO609_003260"
     CDS             523..720
                     /locus_tag="OO609_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634571.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(670..1863)
                     /locus_tag="OO609_003261"
     CDS             complement(670..1863)
                     /locus_tag="OO609_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6634572.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1999..3723
                     /gene="iucA"
                     /locus_tag="OO609_003262"
     CDS             1999..3723
                     /gene="iucA"
                     /locus_tag="OO609_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6634573.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3724..4671
                     /gene="iucB"
                     /locus_tag="OO609_003263"
     CDS             3724..4671
                     /gene="iucB"
                     /locus_tag="OO609_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6634574.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4671..6413
                     /gene="iucC"
                     /locus_tag="OO609_003264"
     CDS             4671..6413
                     /gene="iucC"
                     /locus_tag="OO609_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6634575.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6410..7747
                     /gene="iucD"
                     /locus_tag="OO609_003265"
     CDS             6410..7747
                     /gene="iucD"
                     /locus_tag="OO609_003265"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6634576.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7753..9948
                     /gene="iutA"
                     /locus_tag="OO609_003266"
     CDS             7753..9948
                     /gene="iutA"
                     /locus_tag="OO609_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6634577.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10499..10642)
                     /locus_tag="OO609_003267"
     CDS             complement(10499..10642)
                     /locus_tag="OO609_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634578.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10881..>11511
                     /locus_tag="OO609_003268"
     CDS             10881..>11511
                     /locus_tag="OO609_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6634579.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGES"
BASE COUNT         2908 a         2867 c         2966 g         2770 t
ORIGIN      
        1 aaagaaaata tcactgaaag agctcagcag tttaaaagag ttttagtttt acaggataca
       61 acagaactta attattctgg tcagaaagaa aaacaaggag ttggtcccaa aaaacataaa
      121 gatgaaagaa acttatttct tcatcctcaa ctcgtcatca gtgagtcagg tgtttgcctc
      181 ggtgtttatg atgactatca gtggttcagg gatgaattaa aaaccagtaa aaatacccga
      241 caagaaatct gcaatgattt acttcataaa aaacatgtca gtgaaaaaga aacctggcgt
      301 tgggttgaag gatataataa agcaacagaa ctggctcgcc aatgccctga tacccatgta
      361 ttgtcaattt ctgatcgtga aggcgatttt tatgacctgt ttgaacgcgc agcgcagact
      421 cccggaataa aagctgactg gcttgtcaga atgaaattta ataacagagc aacgctgaat
      481 attaatggta aacgggatca taggttgttg cacgaaagaa tcatggagat tacaccgcag
      541 caactggttg aattcactat acctgatggg cgggggcagc aagccagaac actgcctgac
      601 atgctttcat cagacgcagg tcctctctca tatcctctga tttactccag taaggtgatg
      661 atcatatctt tatttgatag tacggtaatg ccagataaat acagacacaa tggtaaataa
      721 tatagttgca aataatgctg ccataatcag cgcgattgcg acacctgttt tcgataccag
      781 cggcaccagc aaagcgcaca gtccatagcc aagtgtgtga ctggtagcga gccagcccgc
      841 gcctgcgcca tcagccagct tatcattcag tagaagttga taagcgggtg ttgccagagc
      901 tgctccaaat gacagcactg cacagcccgg gtaaaacaac catagctgtt cggagagcat
      961 gatagccaga ccaccactca tcaacactcc ggcactcagg agcagggcca ccggagtcag
     1021 gcgctgcgga cggagtaccc cgaactgcgc gataagcgca gctactgcgg acagacccaa
     1081 cagccaggcc acctgttggc taatggcggt ggtatccgta gcgaactggc gagtaagggc
     1141 aggcgaaagt ccaagctgca tcatgctcac cgccgcagcc agtaatattg cgcaaagcag
     1201 ataaggcaga caatcccgct tcagactgac gctcttgcac tccggtgtgg gctgtggtgg
     1261 tgtgccgggc aaccgcagca gcatcagcag cgccagcact ggggctgcca tcagcagccc
     1321 cagtggcgcc agaggatgaa tggccagcat tgctgccgcg cacagcggcc cgaatagcct
     1381 gccgcaactc aggccggagc tgatggttgc cagagcggcc atgcgattcc cttctccagc
     1441 tctctgcaat gcccagacct gacaggctgg caccattgcc gacacggtca gcccgtaggc
     1501 gatccgggcg atgattaata ttcccagtcc caccgctgtt gttatggcgc tggtagccat
     1561 cagcacgctt cccagcccta gcagggtaaa gcttatgcca tatcctccca gagcccacaa
     1621 caccacaggt ttactgccag tacgggaaat ttgctttccc caccacgggg acgatggcag
     1681 aaacagcatt gagccaagca tcagcagggc agcccataca gacagactca gatttgtctg
     1741 tatgaccaga acagggagca caaccagcag accgttctgt ccgataccga gaagcccggc
     1801 actgaacgca agtggccagc aggacagtgg tttttggggc gtatcttcga tccctggtga
     1861 cattataatg tttcaactcc atgtattaat tgtgtttatt tgtaaaatta atttatctga
     1921 caataacatt tattgttgat aatgagaatc attattgaca taattgttat tattttactg
     1981 tgtgggagct gtttgactat gaccctgccc tctgaaaaac cagccacaga tgtggctgcg
     2041 cagtgcttcc tgaatgcact gattcgtgaa accacagact ggaaactgac agaatacccg
     2101 ccagacgaat tgctcatccc gctggatgag cagaaatcgc tccatttcag agtggcttat
     2161 ttctccccaa cccagcatca ccgctttgca tttcctgcac gtctggtcac ggcatcaggc
     2221 agttatcctg tcgactttac cactctctcc cggcttatta ttgataagct acgccatcaa
     2281 ctgtttctgc ccgttcccct ctgcgaaact ttccaccagc gcgtgctgga aagccatgtc
     2341 catacgcaac aggcaattga tgcccgtcat gactgggccg ctctgcgtga aaaagcgttg
     2401 aattttggcg aggctgagca ggcgctgctg acaggacacg ctttccaccc tgcgcctaag
     2461 tctcatgaac cgtttaaccg gcgggaggct gaacgctacc tgcctgatat ggcaccccac
     2521 ttcccactgc ggtggttttc ggtggataaa acgcaaatcg ccggtgaaag tttgcatctc
     2581 aaccttcaac agcggctgac gcgatttgcc gcagagaatg cgcctcagtt actcaacgaa
     2641 ttaagtgaca accaatggct gttcccgttg cacccgtggc agggggaata tcttttgcag
     2701 caggggtggt gccaggcact tgttgctaaa gggctgatta aagacttagg tgaggccggc
     2761 acgtcgtggc tgccgaccac ctcttcccgt tccctctact gtgccaccag ccgcgatatg
     2821 atcaagttct ccctgagcgt acggctgacc aactccatcc gtactctgtc cgtgaaagaa
     2881 gtgaagcgtg ggatgcgcct ggcacgcctg gctcaaaccg acggctggca gatgctacag
     2941 gtccgcttcc cgactttccg ggtaatgcag gaggatggct gggccgggct gctcgatctt
     3001 aacggcaaca tcatgcagga aagtctgttt gccctgcgtg aaaatctgct ggtggatcag
     3061 ccgaaaagcc agactaacgt actggtctcc ctgactcagg ccgcacctga tggcggtgat
     3121 tcgctgctgg tttcggcggt aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag
     3181 gccgcccatg catgggttga tgcatactgt cagcaagtgc taaagccgct gtttacggct
     3241 gaagcggatt acggcctggt gctgctggcg catcagcaaa atattcttgt ccagatgctt
     3301 ggggatctgc cggtcggatt tatttaccgt gactgtcagg gcagcgcttt tatgcctcac
     3361 gcgacagact ggctcgattc tattggcgag gcgcaggcgg aaaatatttt cacccatgag
     3421 cagttgctgc gctatttccc ttattacctg ctggttaact ccacttttgc tgtgaccgct
     3481 gcgctggggg ctgccgggct ggacagcgaa tcgaatctga tggctcgtgt acgagcatcg
     3541 ctggctgaag tgcgtgatca ggtgactcat aaaacctgtc tcaactacgt gctggaaagt
     3601 ccgtactgga acgtaaaagg taactttttc tgttatctga acgatcataa cgagaacacc
     3661 attgttgacc cttcggtgat ctatttcgat ttcgctaacc cgctgcaggc tcaggaggtc
     3721 tgaatgtctg aggcaaacat tattcacagc agatatggac tgcgctgtga aaaactcgac
     3781 aagcccctga atcttggctg gggactggac aatagcgcgg tgttgcactg tcccggggag
     3841 ctgccgacag ggtggctgtg cgatgcgctg gatcagatat ttatcgccgc accacaactg
     3901 tcagcagtgg ctctcccctg ggccgaatgg cgtgaggagc cacaggcgct gacgcttttt
     3961 gggcaggtaa aaagcgacat tatccatcgt actgccttct ggcagttacc gttatggttg
     4021 agttcaccgg caaaccgggc ctccggtgaa atggtttttg atgcagagcg tgagatttat
     4081 ttcccgcagc gcccaccccg tccacagggt gaggtttacc gccgttacga tccacgtatt
     4141 cgcaggatgc tgagtttccg cattgccgat cccgtttctg atgcagaacg tttcacccgc
     4201 tggatgaatg atccgcgcgt tgagtatttc tgggagcaaa gtggctcact ggaggtacag
     4261 accgcctatc tggagcgcca gctcaccggt aaacatgcat tcccgctgat tgggtgcttc
     4321 gacgatcggc cattcagcta tttcgaaatc tactgggcgg cggaagaccg cattggtcgt
     4381 cactattcgt ggcagccctt tgaccgtggc ctgcatctgc tggttggtga acagcaatgg
     4441 cgcggggccc actatgtgca aagctggctg cgcgggctga cacattacct gctgctggat
     4501 gagccccgta cgcagcgcac agtactggag ccacgcaccg ataaccagcg cctgttccgc
     4561 catcttgagc ctgcgggata ccggacaatt aaagagttcg acttcccaca taagcgctcg
     4621 cgcatggtga tggcggatcg tcatcacttc ttcacggagg tcggtctgta atgaatcaca
     4681 aggactggga ttttgttaac cgtcggctgg tggcaaaaat gttgtctgag atggagtatg
     4741 agcaggtttt ccacgcggaa tctcagggcg atgaccacta ctgcattaac ctgccgggag
     4801 cacaatggcg cttcatcgct gaacgtggta tctggggctg gctgtggatt gatgcccaaa
     4861 cgttgcgctg cacggacgaa ccagtactgg cgcagacgct gctgatgcag ctaaagccgg
     4921 tgctgtcaat gagtgatgcc actgttgcag agcatatgca ggatttgtat gccacgctac
     4981 tgggcgacct gcaactgctg aaggcccgtc gcggactgag tgccagtgac ctgattaatc
     5041 ttgatgccga tcgtctgcaa tgcctgctga gcggtcatcc taaattcgtt tttaataaag
     5101 gtcgccgtgg ctggggcaaa gaggcgctgg aacgatatgc gccagagtat accaacacct
     5161 tcagactgca ctggctggcg gtaaaacgtg aacatatgat ctggcgctgc gataacgatc
     5221 tggatattca gcagttgttg actgccgcaa tggatccgca ggagtttaca cgcttcagtc
     5281 aggtctggca ggaaaacggg ctggatcaca actggctgcc gctgccggta catccgtggc
     5341 agtggcagca aaaaatcgcc acagacttca tcgctgattt tgccgaaggc aggatggtgt
     5401 ctctcggcga gtttggcgac cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg
     5461 ccagccgccg gggagggctg gatatcaagc tgccgctgac catctacaat acctcatgct
     5521 accgggggat ccctggcaga tatatcgctg ccggaccact ggcttcacgc tggctgcaac
     5581 aggtttttgc gaccgacgcc accctggtgc aaagcggcgc agtgatcctt ggtgaaccgg
     5641 ctgcaggcta tgtgtcccat gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc
     5701 aggaaatgct tggtgttatc tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa
     5761 gtcctgttct gatggcaaca ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg
     5821 catatatcga ccgctccgga ctggacgctg aaacctggct tacgcaattg ttccgggtgg
     5881 ttgtggttcc gctgtatcac ctgctttgcc gctacggtgt cgcgcttatt gcacacggac
     5941 aaaatataac gctcgccatg aaagaggggg taccacagcg tgttctgctg aaagacttcc
     6001 agggcgatat gcggctggtg aaagaagcgt ttcccgaaat ggactctttg cctcaggagg
     6061 tgcgtgatgt cacatcccgt ctgagtgcgg actacttaat ccatgatttg cagacgggtc
     6121 acttcgtgac agtactgcgt tttatttcgc cactgatggt tcgtcttggc gtacctgaaa
     6181 ggcgatttta tcaactgctg gcagcagtgt tgagtgatta catgaacaaa catccacaaa
     6241 tggcagagcg ttttgcgctt ttctcactct tcaggccaca aatcattcgc gtggtgctga
     6301 acccggtaaa actgacctgg ccggacctgg atggcggcag tcgcatgctg ccgaattacc
     6361 ttgagaatct gcaaaatccg ctgtggctgg taactcagga atatgaatca tgaaaaaaag
     6421 tgtcgatttt attggtgtcg ggacagggcc atttaatctc agcattgctg cattgtcaca
     6481 tcagatcgaa gaactgaact gtctcttctt tgacgaacat cctcattttt cctggcatcc
     6541 gggtatgctg gtaccggatt gtcatatgca gaccgtcttt ctgaaagatc tggtcagtgc
     6601 agttgcacct acaaatccct acagctttgt taactatctg gtgaagcaca aaaagttcta
     6661 tcgcttcctt acaagcaggc tacgtacagt atcccgtgaa gagttttctg actatctccg
     6721 ctgggctgct gaagatatga ataacctgta tttcagtcat accgttgaaa atattgattt
     6781 cgacaaaaaa agtcgattgt ttctggttca gaccagtcgg ggagaatatt ttgcccacaa
     6841 tatctgcctt ggtacaggaa aacaacctta tttaccaccc tgtgtgaagc atgtgacaca
     6901 atcctgtttc catgccagtg aaatgaatct tcgtcggccg gacctgagtg gaaaacggat
     6961 aaccgtggtt ggtggaggac agagtggtgc agacctgttc cttaatgcat tacgcgggga
     7021 atggggagaa gcggcggaaa taaactgggt ctcacggcgt aataatttta acgcactgga
     7081 tgaggctgct tttgctgatg agtattttac acctgaatat atttcaggct tctccggact
     7141 gaaggaagat attcgccatc agttactgga tgagcagaaa atgacatcgg atggcatcac
     7201 tgccgattct ttactgacca tttatcgtga gttgtaccac cgttttgaag ttctgagaaa
     7261 accaagaaat atccgtctgc tacccagtcg ctcggtaaca actctggaaa gtagtggtcc
     7321 gggctggagg ttattgatgg aacatcatct ggatcagggc agagagagcc tggaaagtga
     7381 tgtggtgatt ttcgccacag gttaccgttc tgcattgcca caaatacttc cctcactgat
     7441 gcccttgatc accatgcacg ataagaacac ctttaaagtg cgtgatgact tcactctgga
     7501 atggagtggc ccgaaagaaa ataacatctt tgcggtcaac gccagcatgc aaactcatgg
     7561 catcgccgaa ccccagctca gcctgatggc ctggcgatct gcacgtattc ttaatcgcgt
     7621 actggggcgt gatttattcg atctcagtat gccgcccgca ctgattcagt ggcgcagcgg
     7681 cagccggaaa aaaccacagc cggaggctgc ttctttaact cactatacag caaatattca
     7741 ggaataacga tgatgcgcaa aaagtatatg ccccgggctc ttggtccgct gcttcttgtc
     7801 gtgctgtcac cagctgtcgc ccagcaaaac gatgataatg agatcatagt gtctgccagc
     7861 cgcagcaatc gaactgtagc ggagatggcg caaaccacct gggttatcga aaatgccgaa
     7921 ctggagcagc agattcaggg cggtaaagag ctgaaagacg cactggctca gttaatcccc
     7981 ggccttgatg tcagcagcca gagtcgaacc aactacggta tgaacatgcg tggccgcccg
     8041 ctggttgtcc tgattgacgg tgtgcgcctc aactcttcac gttccgacag ccgacaactg
     8101 gactctgtcg atccttttaa tatcgaccat attgaagtga tctccggcgc gacggccctg
     8161 tacggtggcg ggagtaccgg agggttgatc aacatcgtga ccaaaaaagg tcagccggaa
     8221 accatgatgg agtttgaggc tggcacaaaa agtggcttta acagcagtaa agatcacgat
     8281 gagcgcattg ccggtgctgt ctccggcgga aatgaccata tctccggacg tctttccgtg
     8341 gcatatcaga aatttggcgg ctggtttgac ggtaacggcg atgccaccct gcttgataac
     8401 acccagaccg gcctgcagca ctccaatcgg ctggacatca tgggaaccgg tacgctgaac
     8461 atcgatgaat cccggcagct tcaactgata acgcagtact ataaaagtca gggagacgac
     8521 aattacgggc ttaatctcgg gaaaggcttt tccgccatca gcgggagcag cacaccatac
     8581 gtcagtaagg ggctgaattc tgaccgcatt cccggcactg agcggcattt gatcagcctg
     8641 cagtactctg acagtgattt cctgggacag gaactggtcg gtcaggttta ctaccgcgat
     8701 gagtcgttgc ggtactaccc gttcccgacg gtaaatgcga ataaacaggc gacggctttc
     8761 tcctcgtcac agcaggatac cgaccagtac ggcatgaaac tgactctgaa cagccaactg
     8821 atggacggct ggcaaatcac ctgggggctg gatgctgagc atgagcgctt tacctccaac
     8881 cagatgttct tcgatctggc tcaggcaagt gcttccggag ggctgaacaa ccataagatt
     8941 tacaccaccg ggcgctatcc gtcatatgac atcaccaatc tggcggcctt cctgcaatcc
     9001 agctatgaca ttaatgatat ttttaccgtt agcggtggcg tacgctatca gtatactgag
     9061 aacagggtag atgatttcat cgactacacg cagcaacaga agattgctac cgggaaggcg
     9121 atatctgccg acgccattcc tggtggttcg gtagattacg ataactttct gttcaatgct
     9181 ggtctgctga tgcacatcac cgaacgtcag caggcatggt tcaatttttc ccagggggtg
     9241 gcattgccgg atccggggaa atattatggt cgcggcatct atggtgcagc agtgaacggc
     9301 catcttcccc tgacaaagag cgtgaacgtc agcgacagta agctggaagg cgtgaaagtc
     9361 gattcttatg aactgggctg gcgctttatc ggtgacaacc tgcggactca aatcgcggca
     9421 tattactcgc tttccaataa gagcgtggaa aggaataaag atctgaccat cagtgtgaag
     9481 gacgacaggc gccgtattta cggcgtggaa ggtgcggtgg actacctgat cccggatact
     9541 gactggagta ccggtgtgaa cttcaatgtg ctgaaaaccg agtcgaaagt gaacggtcaa
     9601 tggcaaaaat atgacgtgaa ggaatcaagt ccatcgaaag cgacagctta cattaactgg
     9661 gcgccggaac cgtggagtct gcgtgtacag agcaccactt ctttcgacgt aagcgatgca
     9721 gagggtaacg atattaatgg ttacactacc gtcgatttta tcagtagttg gcagcttccg
     9781 gtgggaacac tcagcttcag cgttgagaac ctcttcgacc gtgactatac cactgtctgg
     9841 ggacagcgtg cacctctgta ctacagcccg ggttacggcc ctgcttcact gtacgactac
     9901 aaaggccggg gccgaacctt tggtctgaac tactcagtgc tgttctgacg gggatgcttt
     9961 tgcaacaagg gtatgctgta aacaacatgg tccttggtgg ccatgttgtt tacagccagt
    10021 ataatataca gccttaacaa gatattccgt ctccggaatg ggctgttcac cactgaataa
    10081 cattatgtct gttttatgga tctttgactg aaatttatcg catatcctca tttcattgtc
    10141 cgtctgaaaa ctaaccttac aagtaagcgt cgtctcagca ccgtctggca gatcctgaaa
    10201 ttcctgagag gatgatctgc gcccaatatc ctgagccgga actttgctgc aatgaaggcc
    10261 aacgaaaaat gggggagtat gttatagagt acttgctaca gggtaaaaag aggtacctgt
    10321 cgctggttct ccgttcagtt taaccgggag attttctcct gcgcctgtca taaagtccgg
    10381 taatggagat tgttaatacg atggtgcacg atgcatcgtc agtgcccggc ccggaggatg
    10441 ccccgtagct acatacggat cagggttggc aatatcgaat ggcaggctat aggcaaagtt
    10501 aaagccccat ggagtagcac aaaatatgcc gcacaaagga aacggtctga ataacgcagt
    10561 gatggagaac ttcttcggca cactgcaaaa tcgcatcgtg gtcgatcgct aattcgtcgt
    10621 gaatcactga acgatgttcc atgcgaacag atattcgatc atatcgaaat attaaacatt
    10681 acccctcccc acattgggca aaaaaaaaca aaaaacgatc aattagtatt gattcacata
    10741 atctctcatc attaatgaga acattgtcac atatttacaa tatagaactg ttacttttag
    10801 caagctgtta gtaaccaacc gttactaata attttaattc ttaatacttt gttcattcaa
    10861 taaatgagtt gagagaatat atgaataaaa tatactccct taaatatagt gctgccactg
    10921 gcggactcat tgctgtttct gaattagcga aaagagtttc tggtaaaaca aaccgaaaac
    10981 ttgtagcaac aatgttgtct ctggctgttg caggtacagt aaatgcagca aatattgata
    11041 tatcaaatgt atgggcgaga gactatcttg atcttgcaca aaataaaggt attttccagc
    11101 ccggagcaac agacgtaaca atcactttaa aaaacggaga taaattctct ttccataatc
    11161 tctcaattcc ggatttttct ggtgcagcag cgagtggcgc agctaccgca ataggaggtt
    11221 cttatagtgt tactgttgca cataacaaaa agaaccctca ggccgcagaa actcaggttt
    11281 acgctcagtc ttcttacaag gttgttgaca gaagaaattc caatgatttt gagattcaga
    11341 ggttaaataa atttgttgtg gaaacagtag gtgccacccc ggcagagacc aaccctacaa
    11401 catattctga tgcattagaa cgctacggta tagtcacttc tgacggttca aaaaaaatca
    11461 taggttttcg tgctggctct ggaggaacat catttattaa tggtgaatcc a
//
LOCUS       DAJTYH010000115        11491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTYH010000115 DAJTYH010000000
VERSION     DAJTYH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..304)
                     /gene="csgB"
                     /locus_tag="OO609_003269"
     CDS             complement(<1..304)
                     /gene="csgB"
                     /locus_tag="OO609_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6634580.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSAND"
     gene            1058..1708
                     /gene="csgD"
                     /locus_tag="OO609_003270"
     CDS             1058..1708
                     /gene="csgD"
                     /locus_tag="OO609_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6634581.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1713..2102
                     /gene="csgE"
                     /locus_tag="OO609_003271"
     CDS             1713..2102
                     /gene="csgE"
                     /locus_tag="OO609_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6634582.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2127..2543
                     /gene="csgF"
                     /locus_tag="OO609_003272"
     CDS             2127..2543
                     /gene="csgF"
                     /locus_tag="OO609_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6634583.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2570..3276
                     /locus_tag="OO609_003273"
                     /pseudo
     CDS             2570..3276
                     /locus_tag="OO609_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3409..3900)
                     /locus_tag="OO609_003274"
     CDS             complement(3409..3900)
                     /locus_tag="OO609_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6634584.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4002..4556)
                     /gene="ycdY"
                     /locus_tag="OO609_003275"
     CDS             complement(4002..4556)
                     /gene="ycdY"
                     /locus_tag="OO609_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6634585.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4580..5317)
                     /gene="ycdX"
                     /locus_tag="OO609_003276"
     CDS             complement(4580..5317)
                     /gene="ycdX"
                     /locus_tag="OO609_003276"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6634586.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5372..6310)
                     /gene="ghrA"
                     /locus_tag="OO609_003277"
                     /pseudo
     CDS             complement(5372..6310)
                     /gene="ghrA"
                     /locus_tag="OO609_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6545..6632
                     /locus_tag="OO609_003278"
     tRNA            6545..6632
                     /locus_tag="OO609_003278"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6579..6581,aa:Ser,seq:gga)
     gene            complement(6782..7063)
                     /locus_tag="OO609_003279"
                     /pseudo
     CDS             complement(6782..7063)
                     /locus_tag="OO609_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7345..8349)
                     /locus_tag="OO609_003280"
     CDS             complement(7345..8349)
                     /locus_tag="OO609_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6634587.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8421..8981)
                     /locus_tag="OO609_003281"
                     /pseudo
     CDS             complement(8421..8981)
                     /locus_tag="OO609_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9066..9263)
                     /locus_tag="OO609_003282"
     CDS             complement(9066..9263)
                     /locus_tag="OO609_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634588.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9275..9766)
                     /locus_tag="OO609_003283"
     CDS             complement(9275..9766)
                     /locus_tag="OO609_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634589.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9763..10137)
                     /locus_tag="OO609_003284"
     CDS             complement(9763..10137)
                     /locus_tag="OO609_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6634590.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10184..10558)
                     /locus_tag="OO609_003285"
     CDS             complement(10184..10558)
                     /locus_tag="OO609_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6634591.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10721..10942)
                     /locus_tag="OO609_003286"
     CDS             complement(10721..10942)
                     /locus_tag="OO609_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6634592.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11005..11481)
                     /locus_tag="OO609_003287"
     CDS             complement(11005..11481)
                     /locus_tag="OO609_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6634593.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
BASE COUNT         2989 a         2782 c         2702 g         3018 t
ORIGIN      
        1 catcgttggc actgcccgcc tgatcaatat atgcaaggtt ataatctcct gtctggtcaa
       61 tctttgcccg attgctacta ccttcttgcg caacaaccgc caaaagtttt gagcctccct
      121 gccgtaactg agcactatta ttagtcccag cctgaccaat tatggctgcc tgattaaatg
      181 aagacttact caattcattt accgcgaagt tatattctga attagctaaa tcataacctg
      241 ctgcggctgc aatcccaggc gcacccagta ttgttaacat cataaataac aatttgtttt
      301 tcatgttgtc accctggacc tggtcgtaca tttaagaaat taaatcattt caacttggtt
      361 gttaacgcaa cctgtatttt gttaacgctg cgttacgatg gaaagtatgt ctgcggaaat
      421 atttttaata actcacccgc gcgttgtatt tttctttttt tgttcatacc aaagtattaa
      481 aacccgcgta aatacgctgt tatctacgca aaaaatattt tgttttctct taaatctccg
      541 ttttccgcta tcaaaaatca ccagacagtc attcttcttg cccgtcgctg attgctgcga
      601 tatgtcttgc gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa attgtccacg
      661 gaggtgtgga gaaaaaacaa aaacgtttta catgacgaaa ggactacacc gaaatatttt
      721 ttatatgtat tattagtaag ttagcaccat ttgtatgatt ttttaaaatt gtgcaataaa
      781 aaccaaatgt acaacttttc tatcatttct aaacttaata aaaccttaat gttaacattt
      841 taatataatg agttacattt tgttacatgt ttaacacttg atttaagatt tgtaatggct
      901 agattgaaat cagatgtaat ccattagttt tatattttac ccatttaggg ctgatttatt
      961 actacacaca gcagtgcaac atctgtcagt acttctggtg cttctatttt agaggcagct
     1021 gtcaggtgtg cgatcaataa aaaagtgggg tttcatcatg tttaatgaag tccatagtat
     1081 tcatggtcat acattattgt tgatcactaa accttctttg caggcgacag ctctcttgca
     1141 gcaccttaaa caatcgctag caattacagg aaaattacat aatattcaac gttctctgga
     1201 cgatatctct tcaggctcta ttattcttct ggatatgatg gaagcggata aaaagcttat
     1261 ccattattgg caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa
     1321 tacgcctgaa gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt
     1381 ttatgccatg gaggatcaag aacgtgttgt caatgggttg caaggcgtcc tgcgtggcga
     1441 atgctacttt acgcaaaagc ttgccagtta cctgattaca cattctggta actatcgtta
     1501 taacagcacg gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg
     1561 tatcggcgcg tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa
     1621 aacgcatctt tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc
     1681 ctgggcaaac gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg
     1741 cggcagaatt tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat
     1801 tgttaactga ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata
     1861 aatgggaaag tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg
     1921 gaagctggat cacaataacg gtcaatcagg atgttatttt ccagactttt ttattcccgt
     1981 tgaaaagaga ctccgagaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa
     2041 atcgtcgcca gataaatcag gcgttattaa gtacggacga tttggcgcat gatgaattct
     2101 aaataaaaaa ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc
     2161 ttatttcgcc attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg
     2221 gtggtaaccc aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata
     2281 aagatccgag ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta
     2341 ctcaggccat ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac
     2401 cgggccgcat ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc
     2461 agttgaacgt gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac
     2521 aaaataactc aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt
     2581 atttcttttg gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc
     2641 cgccagaccg acattaatgc ctcgtgctca gagctacaaa gatttgaccc atctgccagc
     2701 gccgacgggt aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa
     2761 accctacccg gcaagtaact tctccactgc tgttccgcaa agcgccaggg cttacaaaac
     2821 ctgcttaacg agcgcaaggt tattcgtgcg gcacaagaaa acggcacggt tgccattaat
     2881 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatta tggttgaagg ttcgattatc
     2941 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     3001 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     3061 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     3121 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     3181 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     3241 aatgatggta tcgaccgtgg tctgtgggat ttgtaaaata aagcagaacg gcagaatgac
     3301 attctggtga aataccgcca tatgtcggtt ccaccggaat cctgacagat aaaaatgccg
     3361 gaattaataa tccggcattt actgtttaag cgaaagcgtg agtgcccctc acgctttatt
     3421 tttgatttcc tgctcacggt gagccgtctt tgcactacgc gccgccagcc acaggccgct
     3481 gtttttcatt gcgtaaccaa ataccagccc aagcgccagc gaaggtaaca ccagtttcca
     3541 gtcaccctgc ccggcaaacg tcgcgcatgc gcctataaac gtccccggaa caaatgaaag
     3601 cagcagctgt ttggcctgaa tacacatcag aaacgcgaca ataccggtta tgacataacc
     3661 gagaatttcc agatgtggtg ccagtgcact accgtaaata atgaccatcg cccacaccac
     3721 gccactaagc agggttgcag cggagatcgc cagccctttc agcccacctt gcgggcaagc
     3781 aaagtaggcc gtacagccca ggaagcccgc ccagcttagt aagcccaggg aaacagccac
     3841 ccatccccag ataccggaga gaatgcctgt tgtgattgca atagagagaa gtatgttcat
     3901 gccgcgcatg atagcagaaa acgcggccat gaatgagatc gagcacacat tttatgcaac
     3961 aacaaattat ctgttgcagt tatgagtgac gatgatcaca tttactcttc agaatcttct
     4021 tccagctcgt cccacattgc actaatggca tcacgggtta gcggtgccat ggtgcgccag
     4081 aaaggcgtgg ttgcatgggc ctccactttg ccgaggaacg caccacacca gggtaacaga
     4141 tactcactga acagtgtttc cagtgcttcg ctctcatctt ccgttgactg atcttccagc
     4201 caggaagccg caagcagcaa tgtgccgatg tgatcggctg gcgtatccgc cagtagcatc
     4261 cctcgttcgg aaagaaaagc gcgcacttcc gcttccgttg cgtcctcaac ccatgcgcta
     4321 cgatatggcg gcacggcaca ttcatcgccg ataaacaacg cattgtaatc ggcagagact
     4381 tgcgtcatat cacagctttt ctgtaaacgc gtcagtaact catcctgctc cagtggccag
     4441 ttcgcagcca gtttcccctc acgaatcaga gtaaacagcg gcaccagtaa aggatcttgc
     4501 ggttggcggt aatagagcga acccagcaca cgacagagga tagaaaactc gttcattaat
     4561 atatttccat tactagtaat taaaggtctg caaattccgc aatcggtgcc ataccgcgag
     4621 attcaaggaa gttcagtaag cggcgcggag aaacattcaa aatgcgctct agcggaaaat
     4681 ctaccgcgtc gaggatttta agacactctt caaattcccc catggtaaac gcggtgtgag
     4741 aatccgagcc taatgccacc caacctccag catcacgtac cgctgcagct accgcacgac
     4801 agttgtcttc actgccctta cgtgagtgta aaaatgagga attattgatt tccagcgcca
     4861 cctgatgttt cgcggctgct tcagcaacgg ctttaacatc tatttcatat ttcggatttc
     4921 cgggatggct tattatatgc acattgccgc tggcgatagt ggagatcatc gcttgtgtat
     4981 tggtcgcttt gtcatgtggc gcaaaaaccg gctcatgaaa accggcaata atcagatcca
     5041 gcgagtcaaa cattttaccg ctgcagtcaa tttcaccatc aacattttta atgttagctt
     5101 caatgccgcg caggatccct accccatcaa ccactcgcgg ccagatacgc atgttaatga
     5161 agtgccagtg atgcggcgca tcttccatat ccgggccatg atcggtgatc gcaaaaagtt
     5221 taatgccctt ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat gcatgtgtgc
     5281 tggcaacggt atgcatatga aggtcgacgg gatacataac tctctcctgt attcaattcc
     5341 cgccaaggat agcaggaatc ctgatgcttt attagtagcc acgtgcgcgg tcgacttgcc
     5401 cgcagaccct ctcccctttt tcgagctggg caatagtgcg agaaatgtac tccacagctt
     5461 cagcgggacg ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga
     5521 gcggactttc aggcggtaag ggttcacgat taaaaacatc cagcattgcg cctttaactt
     5581 tcccgctatc cagcgccgcg agcaggtcat cttccacaac atgaacacca cgcgccaggt
     5641 tgaggagata cgcgccatca ggtaattttt cgagtaattg ttgattaata atgccgacgg
     5701 tttcaggggt attcggtaac aaattaatca atacccgaca ttggctcaga aatgcagaca
     5761 gttcttcccg tccggcaaag ctttgcacgc caggccacga tttacgggtt cgactccagc
     5821 aacgcagcgg aaagcgctag gtttgcagac tctgagcaac tttacagccc agtacgcctg
     5881 cgcccaaaat gccgatggta aaatcttccc gatgatattc aggcagcggt tgccaatgcg
     5941 aactattttg ctggatgcga taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga
     6001 cagcatattc ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa agtggaacag
     6061 aagggttcag catttcaggg tgtgcctgta gcttgctcaa aatagaatca acaccggccc
     6121 cgagtgcgaa caccgcttta agatcgcgcc ctgccagcat ttcaacagga ggatgccaga
     6181 ctaaaacata atcagcagag tcattatctc cgcttttcca tgccctgact cttgcctgag
     6241 gaatagcttt gcgcagtgcc tcaatccacc attgggtatc gaacgttggg tgataaaaga
     6301 tgatatccat actgactccc gaaaagcgtt gtgcgaaatt tattcgcact tatcgttatg
     6361 atctacaaag gccaccagca taacaaatcc gtggtcggtg gcaaaaaaag cagatttcgc
     6421 ttattaaaac cacacattga ttgaatgttt gaataaacgc gcgatttttt caaaaagttt
     6481 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     6541 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     6601 tatcgggggt tcgaatcccc ccctcaccgc cagattataa gtgcatgtta ttgtttaaca
     6661 tgcgcttttt tatttttcct tccagacagt atgccatcgg gtatgccatc tgaaaccggt
     6721 ttatatgccc cgcgatcata tcttatcgga caatttccga tctttgagca tactggcaaa
     6781 gctatactgc cagcatatca ggatagtgtt ttgcgataat atcattcagt ctgtcaacca
     6841 gttctggttt tctgaacgtg atatgcgcgg agcccttctt aaagtatctg atgctgaaca
     6901 tctcatcttc atagctctct ttaccctgaa cagactgaat gtgatcatcc agacgaaggg
     6961 tgatgtttcc ccggttgtcg gggattggtt taccgctgaa caggtgcaac attctttcca
     7021 gatcggcgag tcggtcagcc tgttgtccgg tgataagatg aaatctatgg tcacccccgt
     7081 ttttgcaaca ctgatttttg attaatggtt ggcttgctta aatctatccg gcgtctctgt
     7141 gggcaaggat gcccgcgccc cgatgagttc cgcacctgat aatcctaaaa atcccctcgg
     7201 cttcagtgag ccatttcatc tgttaggttt ttaatcaggt cgatttgccg ttcatgtcat
     7261 caataatcag tcttcgcaaa accagaaagg taacttgtat cttattgttt aaaataaact
     7321 aattgagtta tatcagcatc tatatcaggc ctgatattct tttttctgag tagtgattgc
     7381 ccaggcaatt cgagccagtt tatttgccag agcgcaggta accacattcg aatgcttctt
     7441 gttgagttgc tcccgaaccc actcagcaag ttttccatgc ttgcgttcaa gtcgcattat
     7501 gaatgagcgg gcacactgaa ccagtaaccg gcgtaaattt ttatcacctc gcttactgat
     7561 acccaacaga gtcgattttc ctccagtgct atactgccgg ggaacaagtc ctgtggaagc
     7621 tgcaaaatcc ctgctgcatg agaactgttt cccgtcacca agttgcgacg atagtaagct
     7681 ggcagtaatc ggaccaacac caggaattgt cataatgcgc tgagcggtat catcagcgtt
     7741 gattgactga cttaattctg attccagctc tgctatttgc tcaacaaggt aaaagtagtg
     7801 ggtatgcaac ctcaccaaaa ggcggctcag atattcgggg atttcgtgtt cagccaacac
     7861 gagagacaac cgttttatta cagcatcacc tatcggcagg ctgattccaa attccagcaa
     7921 gaaaccgtgg atctggttgg ttgttttcac cttatctctg atgagcgatt ctctgacccg
     7981 gtgtaacgcc ctcatggcct gctgggtctc agttcttggc tggacaaaac gcattgatgg
     8041 tcgtgaagcc gcttcgcata tggcttctgc atcaacaaaa tcattcttat tacttttgac
     8101 aaacgggcga acgaactgtg gagaaatcag cttagtttca tgcccaaaat cagagataca
     8161 gcgagccata aagtgtgcac ccgcacaggc ttccattaca acaactgagg gcggacatgt
     8221 cgccagaaac tgcatcagtt tagtacgggt aaatttttta cgcagaagag ccttaccaga
     8281 cttgtcttga caatgaatgt ggaaggagtg ctttccgaga tcgataccaa taagtgtgat
     8341 gttctgcatg atggtctctc cgttaaaggg atttacctta acagcgtact gctgttaagg
     8401 tgggggtgac catatcatta tccccaccgg tcccatctca ccagattgtt gacgataatt
     8461 ttactgccaa atttacaggg gcaattggtt ttgtaattcc agttcagccc tctgaagacg
     8521 ttgatcaccc ctcgctcaaa cacctcatcc ttattctggt gcagttgttc aaatgtgctc
     8581 agaatgttcg cttcgctgat ttccggaaaa ttatcgtact ccagtgaccg gtaccatgta
     8641 tcacgggcct gcgtatccat taatgacagc atcccggaac gttgcatcag gtcacgccag
     8701 atttcgcgat caagattccg ggtaatggct ttcattgctg tttcgggttt ctccatcagc
     8761 cagcagccgc agcgaaaatc ctgtttcatg gcccagtccg gggcagtttt acctccgatg
     8821 ctttccgtca gcaccgagat ttcagccagt tgtcggataa gtgtgtcgat ttgcaacagt
     8881 gtactctttc gtacagccag aatgtggcga atgctggttg agcagataac gtccgtatgc
     8941 ccgttgacca cttccggacg ggagattgtg atatctgaca taatcgatat tcctttttgc
     9001 agataaatga aaacgccagc cctgtgcagg ctggcgtcag aggaatgaag tctggtgagt
     9061 gagcttcacc ggttctggtg cagaagctgc agatgacgca gggtacggac atcctcccgg
     9121 gcctgttcga tacagggcaa cagtgctgct gaatccgttt tatcctcatc gttgtcaaag
     9181 ataattcccg atccgcagtc gatattgtcc tgcagccacg caatcagaat atccagcgct
     9241 gtttccgtgg ttaatgattt catgttgttc ctggttacca gtcgaaagtg ggtaaacccg
     9301 gcaggcattc agcgctggca tccagatgaa tgagactgac gccataacgc cggatgagtg
     9361 tgacgaccag acgacggaac gtttttgaca gccccaggcg tttaagccgc agaaccgggt
     9421 atgaccacgc atcggtacgt aacagatatc cgctacctgt aaaatgaatc cattctgatt
     9481 caccgaagtc actggtctgg tgtgacagcg agtacaacca ggcgttgtcc gtttccgtga
     9541 tatgtgcggt actgcagcgt atgccggtaa gggtagcaaa tggtggcgtg cattctgctg
     9601 aggtctggtc agattcatca acaacacgaa gggagtagcc gttatcgcag accatgttaa
     9661 tcagttcagc gagctcaata ccatcgacgt caacgacaat gcgccctatg ttcagtgcct
     9721 gtacgttaac gctgtcggct tccagtgtca gggccagttt catcatttcg cctccggata
     9781 cttacccagg gtaatgttat ttaccgttct gtaattgtcg cgggtcatca ggccggtcgc
     9841 ccggcgagcc cggaggatat caatactgtt tattaactga gagcggggac aggtgctgaa
     9901 tcccggctgg tcggtacgca ccagcgcgta tttttccacg agaaaattaa ctgcatcaca
     9961 cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt tcatcgacaa acggtgtgtc
    10021 attcagtgtg aggccataat gctggtccag cagtcggatg agcagtgtct gccagatggt
    10081 gacgggagac gggcagcgag atacctcccg tacatgtgtg tcggataatg ttttcatctt
    10141 aagttctctg taactggtag tgctaaaggt gagagcttcg gggttatgag gtggttgcgg
    10201 gtgctggtgt cggataaaca gccagataaa cgtaaccaca ggagccgagg gtgtcggctt
    10261 cgcaggtcag cccttttgca tacagcgtga cggtatgctg atatcgggga ttcagttcac
    10321 cgctggtgag catgagttcc agttgtttca tcagcagcgg aaaggcctgg tccagatggt
    10381 atgcatctgc gtcgctgaag cggcctctga taccggcgcg gtcggcaagg taatacaggc
    10441 ggttaccctc ttgcaccaga cgagcgccaa aacagggcct cactgtgcag ggaagccccc
    10501 accaggggcg gtcgtgattg tcatcgggat gagttatccc ggagagtttg tctgacacga
    10561 taaaatcctc tctgaaaagt ggttaagaat ggtttaacga tgacaggtca gccggcgcgc
    10621 tgaccggttt cggtgaagat gtatgcattg atgagaatgg cctcatccac agcggcgtct
    10681 gaggtcatct ggtcgtactc gtttcccggg tgacggaaca tcagttcagg gtgacactca
    10741 tcagccggac atacgggcca aaactgtcct tacggcgttc tgcaaacacg gcaagaacgc
    10801 cgggaatatc ctgcacttca cgaccagtat acgcttcagc actgccgtgc cagcgatact
    10861 taccggtaca gaacggaaaa agacgggatg caggatgctg ttggtgaata cgcatggctt
    10921 caccacgggt gatgattctc atgatgtgat acctctgaag acagaagata aaagtgaaaa
    10981 caggtgtgat gtggttgtga cgggttaaag cagaccatgt tcggcaaagg agaaaacctg
    11041 gttgccaccg actatcagat ggtccggcac ccggatatcc accaggccca gtgcctgtac
    11101 cagacgttcg gtgataagcc ggtctgcctt actgggtgtg acttcaccgg acgggtgatt
    11161 gtgcgccagc accacggcag cggcattgtg gtacagggcg cgtttaatca cttcccgggg
    11221 atgaacttcc gtacggttga tggtgccggt gaagagggtt tcaccggcaa tcagctgatt
    11281 ctggttgttc agatacagca cccggaactc ttcccgctcc agtcccgcca tgttcagaat
    11341 cagccattcc cgtgccgcac gggtggaggt gaaggccacg ccgggttcat gaagatggcg
    11401 gtccagggtt tgcagggccc gcagaatgag actgcgctcg ccgggcgtca tgtttccggg
    11461 cagaaaggaa agctgctgca ttgtccctgc c
//
LOCUS       DAJTYH010000116        11473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTYH010000116 DAJTYH010000000
VERSION     DAJTYH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..203)
                     /locus_tag="OO609_003288"
     CDS             complement(<1..203)
                     /locus_tag="OO609_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634594.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGV"
     gene            complement(234..926)
                     /gene="fimC"
                     /locus_tag="OO609_003289"
     CDS             complement(234..926)
                     /gene="fimC"
                     /locus_tag="OO609_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6634595.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1146..1688)
                     /gene="fimA"
                     /locus_tag="OO609_003290"
     CDS             complement(1146..1688)
                     /gene="fimA"
                     /locus_tag="OO609_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6634596.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2169..3035
                     /gene="folD"
                     /locus_tag="OO609_003291"
     CDS             2169..3035
                     /gene="folD"
                     /locus_tag="OO609_003291"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6634597.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3037..3249
                     /gene="ybcJ"
                     /locus_tag="OO609_003292"
     CDS             3037..3249
                     /gene="ybcJ"
                     /locus_tag="OO609_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6634598.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3357..3866
                     /locus_tag="OO609_003293"
     CDS             3357..3866
                     /locus_tag="OO609_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6634599.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(3902..5287)
                     /gene="cysS"
                     /locus_tag="OO609_003294"
     CDS             complement(3902..5287)
                     /gene="cysS"
                     /locus_tag="OO609_003294"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6634600.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5461..5955
                     /gene="ppiB"
                     /locus_tag="OO609_003295"
     CDS             5461..5955
                     /gene="ppiB"
                     /locus_tag="OO609_003295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6634601.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            5958..6680
                     /gene="lpxH"
                     /locus_tag="OO609_003296"
     CDS             5958..6680
                     /gene="lpxH"
                     /locus_tag="OO609_003296"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6634602.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            6798..7307
                     /gene="purE"
                     /locus_tag="OO609_003297"
     CDS             6798..7307
                     /gene="purE"
                     /locus_tag="OO609_003297"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6634603.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7304..8371
                     /gene="purK"
                     /locus_tag="OO609_003298"
     CDS             7304..8371
                     /gene="purK"
                     /locus_tag="OO609_003298"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6634604.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8508..9401)
                     /locus_tag="OO609_003299"
     CDS             complement(8508..9401)
                     /locus_tag="OO609_003299"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6634605.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9398..10045)
                     /locus_tag="OO609_003300"
                     /pseudo
     CDS             complement(9398..10045)
                     /locus_tag="OO609_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10048..11280
                     /gene="allC"
                     /locus_tag="OO609_003301"
     CDS             10048..11280
                     /gene="allC"
                     /locus_tag="OO609_003301"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6634606.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11291..>11473
                     /locus_tag="OO609_003302"
     CDS             11291..>11473
                     /locus_tag="OO609_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6634607.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVD"
BASE COUNT         2767 a         2987 c         2948 g         2771 t
ORIGIN      
        1 tacacgcccg caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg
       61 ccattctcta ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt
      121 aaaatggcaa gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata
      181 tccgtagtag tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt
      241 aagttcactt ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc
      301 tttccctgca ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca
      361 ttccccaact tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga
      421 ttatgaacat tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg
      481 ggtaattctt gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt
      541 tgcaaaacat tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc
      601 atccagaaca gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa
      661 gtattttcgc ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct
      721 tttacaccgc tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta
      781 tttttaatcc ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct
      841 aatgcgatcc ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc
      901 atcaataact ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa
      961 ataaggagta taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt
     1021 taataaagcg gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct
     1081 tcagacaacc ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt
     1141 tttgattatt cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca
     1201 cttgtaccgg tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag
     1261 ttctggttgg ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt
     1321 atttcaatac cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa
     1381 ttatcattga ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca
     1441 ggatcgcagt cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca
     1501 ttgaaaatat cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc
     1561 actgaacagg cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg
     1621 gcagcataac tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt
     1681 aatttcatat atattccttc aatctcattt atcgactcca catccgtata taaccgatta
     1741 ctttatttaa gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt
     1801 gaaaatatgc actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa
     1861 aatatattca ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt
     1921 atattgtcag gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca
     1981 gatttacaac gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg
     2041 gtgcgcctaa ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag
     2101 cgcgccccgc ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc
     2161 tctctctgat ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg
     2221 aagttgctca aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg
     2281 ttgtgctggt gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt
     2341 gtgaagaagt cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg
     2401 agctgctgga gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc
     2461 aactgccgtt accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca
     2521 aagacgtgga cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc
     2581 tgcgtccctg caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct
     2641 tcggcctcaa cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg
     2701 aactgttgct cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc
     2761 atcacgtaga aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg
     2821 gtgactggat caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg
     2881 gcaaagttgt gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc
     2941 ctgttcccgg cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg
     3001 cgtgcgttga atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa
     3061 catccgcacg ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg
     3121 caggcgaaaa tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc
     3181 aaacgctgca aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt
     3241 gttgcctgat ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc
     3301 ctctacgccc cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc
     3361 caaccgttat tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag
     3421 gtctgttatt tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta
     3481 aatttggcgt tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt
     3541 ttgcgtttgt tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc
     3601 tgattcgctg ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg
     3661 taaccactgg cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg
     3721 ctccctggca ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg
     3781 ggcatcaggt gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg
     3841 gaatgttgtg gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca
     3901 attacttacg acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt
     3961 taagacggtc acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac
     4021 gctgttgaat taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt
     4081 gcaggaacgc ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt
     4141 gagatgccat tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg
     4201 ccatatcaaa cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa
     4261 taaagcgcgc ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca
     4321 gcgcagtgta gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt
     4381 tcagctggct gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt
     4441 atttcagcac atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt
     4501 caaccatcac cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg
     4561 tggactgcgc gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga
     4621 tatcaaagtg gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag
     4681 gacgacccgc gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca
     4741 gaacgaagtc catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca
     4801 gctggtcgag atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca
     4861 tcacgtcgct gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt
     4921 caataatttc tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca
     4981 aagcatcaaa atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt
     5041 cgccattttc attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat
     5101 acttcagttt atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg
     5161 tacgcccgtg accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga
     5221 cttccccggc gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt
     5281 ttagcatcga agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata
     5341 tttcccgcgc gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca
     5401 ccaccctata tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa
     5461 atggttactt tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca
     5521 cctgaaacag ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt
     5581 ttccaccgtg ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa
     5641 caaaaagcca ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt
     5701 ggtacgctgg caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc
     5761 aacgtggttg ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac
     5821 tgcgtgtttg ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca
     5881 accggtcgta acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg
     5941 accgttagcg agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag
     6001 aaccggcgat caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg
     6061 cgctgtatat tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac
     6121 tccatcgcca gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt
     6181 tcattcatgg caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga
     6241 cattattgcc ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg
     6301 gcgacacgct gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac
     6361 cctggctgca aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc
     6421 gaatgcgcgc gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta
     6481 accaaaacgc tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc
     6541 atacccatcg cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg
     6601 tactgggtgc ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc
     6661 tgattcattt tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt
     6721 tttccttgct ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca
     6781 caggagtttt aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg
     6841 ggtccaaaag cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg
     6901 tcccgcacca cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg
     6961 ccgaaagcgc cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc
     7021 acctgccagg catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga
     7081 gcgccgcact gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc
     7141 cggtgggtac gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac
     7201 aaattctcgc gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc
     7261 agaccgacga agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt
     7321 cctcggtaac gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc
     7381 tgtctggccg gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat
     7441 taccgctgag atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc
     7501 ggcctttgtg aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct
     7561 tttcgataag ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg
     7621 gcctgcggtg tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta
     7681 tgacggtcgc ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg
     7741 ttacggtgaa tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg
     7801 cgcgcgcggc tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga
     7861 cggtattttg cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc
     7921 cgaagagatg ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga
     7981 gtgttttgtc accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag
     8041 cggtcactgg acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat
     8101 taccgatctg ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat
     8161 tggtagcgat gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga
     8221 caaagaagtc cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc
     8281 gcgtctgact gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg
     8341 cgtgatttgg gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg
     8401 cgtaaacgcc ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc
     8461 aagcgtcgca tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata
     8521 caggtccccg cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg
     8581 ggtttactac ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca
     8641 tcggctttgg caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc
     8701 ggcgttcccc agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca
     8761 ttaatctgct cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct
     8821 gccccatctt ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct
     8881 ttgagcaaca actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc
     8941 acccggcgca aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc
     9001 tcttgttctt ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga
     9061 tcatcaggcg aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt
     9121 gcgctcaagc tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca
     9181 tccagcggat acggttctac ctctttccac gccagattct gtaacgccag cagccccacc
     9241 tgcggcccgt tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg
     9301 ggtacagcac tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg
     9361 agtaaggcgt tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt
     9421 gttggccaag ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca
     9481 gcgaatcaat cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga
     9541 gtggcgaggc gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg
     9601 taacgagttg aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag
     9661 cgccataata ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta
     9721 atccggggcc tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct
     9781 gaaaacagtg acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca
     9841 gtggcccgaa aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag
     9901 gatgcgaagg cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac
     9961 gcccgaggcg aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc
    10021 ataacgcatc gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg
    10081 ctgccctggc tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat
    10141 tcgccggaat ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg
    10201 gaaacacgtt tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca
    10261 caggaagtgg ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac
    10321 gggcaattcg gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc
    10381 gcaccgttac gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg
    10441 tatgtcttct ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat
    10501 atctgtgatg ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg
    10561 ccgaacgccc cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa
    10621 cagggctgtg tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg
    10681 cagcgtcgct atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg
    10741 ggttatcgtc gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa
    10801 gcgaaaaaga tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat
    10861 acggtaaatg tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct
    10921 gccgtgctgc gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa
    10981 atggacattg gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag
    11041 gagctggtcg ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg
    11101 cacagtggtg ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt
    11161 tttatcccca gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac
    11221 cttgccgaag gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa
    11281 ggagtcataa atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa
    11341 ccgtgcgatt gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa
    11401 tattattccg gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc
    11461 ctcttttgtt gat
//
LOCUS       DAJTYH010000117        11245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTYH010000117 DAJTYH010000000
VERSION     DAJTYH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..898
                     /gene="maeA"
                     /locus_tag="OO609_003303"
     CDS             <1..898
                     /gene="maeA"
                     /locus_tag="OO609_003303"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6634608.1"
                     /translation="NDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIA
                     EMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDS
                     DVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATP
                     QDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDE
                     MLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEA
                     LQQAIDDNFWQAEYRDYRRTSI"
     gene            1032..2042
                     /gene="adhP"
                     /locus_tag="OO609_003304"
     CDS             1032..2042
                     /gene="adhP"
                     /locus_tag="OO609_003304"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6634609.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            2188..2472
                     /locus_tag="OO609_003305"
     CDS             2188..2472
                     /locus_tag="OO609_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6634610.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(2700..3353)
                     /gene="fdnI"
                     /locus_tag="OO609_003306"
     CDS             complement(2700..3353)
                     /gene="fdnI"
                     /locus_tag="OO609_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6634611.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(3346..4230)
                     /gene="fdnH"
                     /locus_tag="OO609_003307"
     CDS             complement(3346..4230)
                     /gene="fdnH"
                     /locus_tag="OO609_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6634612.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4243..7290)
                     /gene="fdnG"
                     /locus_tag="OO609_003308"
     CDS             complement(4243..7290)
                     /gene="fdnG"
                     /locus_tag="OO609_003308"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6703..6705),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6634613.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            7522..8403
                     /gene="yddG"
                     /locus_tag="OO609_003309"
     CDS             7522..8403
                     /gene="yddG"
                     /locus_tag="OO609_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6634614.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            8658..9758
                     /gene="ompC"
                     /locus_tag="OO609_003310"
     CDS             8658..9758
                     /gene="ompC"
                     /locus_tag="OO609_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6634615.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            9922..10407
                     /locus_tag="OO609_003311"
     CDS             9922..10407
                     /locus_tag="OO609_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6634616.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            10517..>11245
                     /locus_tag="OO609_003312"
     CDS             10517..>11245
                     /locus_tag="OO609_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6634617.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLI"
BASE COUNT         2908 a         2814 c         2649 g         2874 t
ORIGIN      
        1 taacgatgac attcagggca ctgcggcggt aacagtcggc acactgatcg cagccagccg
       61 cgcggcaggt ggtcagttaa gcgagaaaaa aatcgtcttc cttggcgcag gttcagcggg
      121 atgcggcatt gccgaaatga tcatcgccca gacccagcgc gaaggattaa gcgaggaagc
      181 agcgcggcag aaagtcttta tggtcgatcg ctttggcctg ctgacggaca agatgccgaa
      241 cctgctgcct ttccagacca aactggtgca gaagcgcgaa aacctcagtg actgggatac
      301 cgacagcgat gtgttgtcac tgctggatgt ggtgcgcaat gtaaaaccag atattctgat
      361 tggcgtctca ggacagaccg ggctgtttac ggaagagatc atccgtgaga tgcataaaca
      421 ctgtccgcgt ccgatcgtga tgccgctgtc taacccgacg tctcgcgtgg aagccacacc
      481 gcaggacatt atcgcctgga ccgaaggtaa cgcgctggtc gccactggca gcccgtttaa
      541 tcctgtggta tggaaagata aaatctaccc tatcgcccag tgtaacaacg cctttatttt
      601 cccgggcatc ggcctgggtg ttattgcttc cggcgcgtca cgtatcaccg atgagatgct
      661 gatgtcggca agtgaaacgc ttgctcagta ttcgccgctg gtcctgaacg gcgaaggtct
      721 ggtactaccg gaactgaaag atattcagaa agtctcccgc gcaattgcgt ttgcggttgg
      781 caaaatggcg cagcagcaag gcgtggcggt caaaacgtct gctgaagctt tgcaacaagc
      841 cattgacgat aatttctggc aagccgaata ccgcgactac cgccgtacct ccatctaagc
      901 ctgcacccgg tagtgaaggc taccgggcta tttccctctc cctttttcag atctcatcca
      961 tactgggtag tggcgaataa atctcatttg cctcacctgc tatgcagaac atcatccgaa
     1021 aaggaggaac tatgaagact gcagttgtta cgaaggatca tcatgttgac gttacggata
     1081 aaacactgcg ctcactgaaa catggcgaag ccctgctgaa aatggagtgt tgtggtgtat
     1141 gtcataccga tcttcatgtt aagaatggcg attttggtga caaaaccggc gtaattctgg
     1201 gccatgaagg catcggtgtg gtggcagaag ttggcccagg tgtcacctca ttaaaaccag
     1261 gcgatcgtgc cagcgtggcg tggttttacg aaggatgcgg tcattgcgaa tactgtaaca
     1321 gtggtaacga aacgctctgc cgttcagtta aaaatgccgg atacagcgtt gatggcggga
     1381 tggaggaaga gtgcatcgtg gtcgccgatt acgcggtaaa agtgccagat ggtctggact
     1441 cggcggcgcc cagcagcatt acctgtgcgg gggtcaccac ctacaaagcc gttaaactgt
     1501 caaaaattcg tcccggtcag tggattgcta tctacggtct tggcggtctg ggtaacctcg
     1561 ccctgcaata cgcaaagaat gtctttaacg ccaaagtgat cgccattgat gtcaatgatg
     1621 agcagttaaa actggcaacc gaaatggatg cagatttagc gattaactca cgcaccgaag
     1681 acgccgccaa aattgtgcag gagaaaaccg gtggcgctca cgctgcggtg gtaacagcag
     1741 tagctaaagc tgcgtttaac tcggcagttg atgctgtccg tgcaggcggt cgtgttgtgg
     1801 ctgtcggtct gccgccggag tctatgagcc tggatatccc acgtcttgtg ctggatggca
     1861 ttgaagtggt cggttcgctg gtcggcacgc gccaggatct aaccgaagcc ttccagtttg
     1921 ccgccgaagg caaagtggtg ccgaaagtcg ccctgcgtcc gttagcggac atcaacacca
     1981 tctttaccga gatggaagaa ggcaaaatcc gtggccgtat ggtgattgat ttccgtcact
     2041 aagaggtctt tgctgcgact cccatgttcg ggtcgcagca tcgcacactc tccaacatga
     2101 aatggctaaa tggattattg ttgcgtagga aggaacaata atgatagtgt tcccagagaa
     2161 gtaacgctac tgagagtaaa gacactaatg aaaatggcca atcatccccg cccgggggac
     2221 attattcagg aatcactgga cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg
     2281 gaaattgcgc cctcaacggc aagccgattg ctgaccggaa aagcagcttt gacgccagaa
     2341 atggcaatta aactttccgt ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa
     2401 aacgcctgga gtctggcaga ggcagaaaaa acggtggttg tgtcgcgatt acgccgtctg
     2461 gttacgcaat aaaaaatggc accctataaa gcgccatttg aaactgatga caaacgcaac
     2521 attgcctgat gcgctacgct tatcaggcct acacgttcac ttcaatctat tgtatttgaa
     2581 gggttttcat gccggataag gcattcacga cgcatccggg atgaacaaag cccacgttgt
     2641 ctacaatatg aaaatggcgc cttttacagc gccattttca gcttaaagtt caaaagagat
     2701 tatatccctt cttcactctc ttttttcgct tctgccttct cgatttcacg ataccagcgc
     2761 ggatggtgtt tcttcgccca gcgacggctc accttccctt cgatcatccc tttaatcgac
     2821 cctttcaccc agaatgccat atacatatgg atcaggatgg cgtggatcag gatgataccc
     2881 gcagccgcgt ggatcagcag gctgtagcga acaacctgca tcgggaagta ctgcgcaaag
     2941 tacggacgcc agataatcac cccggtcacc agcagcacga aaatcatgct catgatcgac
     3001 cagaacatca tcttttgccc ggcgttgtac ttaccaacat ccgccacttt gtgctcattg
     3061 ccttttaata cttcgacaat gttcaacagc cacggaatat ctttcttatc cgggatgttg
     3121 tgatgcacaa aacgcacaaa cataaacatc agtgcgacga aaatcgcaat gccgaagaag
     3181 gggtgcaaaa tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca ttgcagcgtc
     3241 gggaagaaga acgaaatccc ggacagcgcc accaggaaga agcaaatcac cacggtccag
     3301 tgacaggcgc gatcaataaa tttggtgcgc acaatcattt tcgacttact catgatgatc
     3361 ctcctcgtca tcgtccactt ccttattcgg gccaataccg atgtagtgga aaatcaaccc
     3421 ggcaaaagtg gcaataaagc cagccgctgc cagcggtttc aacgcgcctt tccacaggct
     3481 taccgaggtg tcgatcttcg gatctttcgg cagaccgtga tacagctccg gctgatcggc
     3541 gtgatgcagc acgtacataa cgtgcgtacc accgacccct tccgggttgt agacgccagc
     3601 atgttcgtaa ccacgcgctt tcagtttcgc cacgcgctgt tccgccagct ccagcatctc
     3661 cttcttggtg ccgaagtgga tagccccggt cggacaggtt ttcacacaag ccggttcctg
     3721 gccgacgctg acgcgatcga cgcagagcgt gcatttatat acccggttat cctctttgtt
     3781 gaggcgcgga atattaaacg gacacccggc aatgcagtaa ccacagccga tgcagttttc
     3841 cgactggaaa tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag acgggcacgc
     3901 cttcaggcag cccggatctt cacagtgcat acagccgtct ttacggatca gccactccag
     3961 cttgccattc tgttcggttt cgctaaagcg catcaccgtc caggacttgg cgctcagatc
     4021 ggcggggtta tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt cgttccactc
     4081 cgaacacgcc acctgacagg ctttacagcc gatacaggtg gaaacgtcga taagtttcgc
     4141 gacttctgct ttgtaatcac gcacctgaga aggcggcgtg atggagttag ttgcggacct
     4201 tttgataata tcctgcgttt ccatagccat ctgttcgccc ccttacgcct tctcgatgtt
     4261 gactaagaac gctttatatt ccggcgtttg cgagtttgca tcaccgacat tcggcgtcag
     4321 agtgttagcg atataacctt tacgcgcgac accctcaaag ccccagtgga ttggaatacc
     4381 caccgtttca acctgctgac cgtttacatt cagcggtttc agacggcgcg ttaccactgc
     4441 caccgcgcgg ataaagcctc gcttgctgga gacagtgaca cgatcgccat tattaatgcc
     4501 tttcgccgcc gcctgcgttt cgctgatttc cacaaactgt tccggctgag caattgcgtt
     4561 gagcaacgcg tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg tcgtacccac
     4621 atacgggaac tgctcttttt tacccatccg cagcgcgtct tgttcataca gacgaacaac
     4681 cgggttagac accacgttcg gatgcagcgg gttagtgccc agcggcgttt caatcggctc
     4741 gtagtgttcc gggaacggac cttccgccat tttgttgatg gcaaacaggc gtcccatccc
     4801 ttccggctgc ataataaacg gcccggttgg cgtacccggt gcggcattgc cgaagtccgg
     4861 aatatcgtta cccgtccact tgctgccgtt ccactggatc agcatccgtt tcggatccca
     4921 cggtttaccg ttgatatccg ccgaagcacg gttgtagagt acgcgacggt tgagcggcca
     4981 cgcccaggcc catcccagcg tattccccag accggacggg tcggagttat cgcggtttgc
     5041 catctggtta ccctgctctg tccagctacc ggtgtagatc cagcaagaag atgcggttgt
     5101 accgtcatca cgcagatgcg caaagctact cagcaactga cctttcttcg caatcagcac
     5161 gccattagca tcatagagat cttccaacgc atagccgttg ttctctttag ctacttcgtc
     5221 agattgcggt tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg gttctacgcc
     5281 tttaccacct tcggcctggt acaactcgcg cagatgatgg tagatacccg ccagaatttc
     5341 gccgtcgtta cgcgcttcgc ccggcgcgtc ctgacctttc cagtgccact gcaaccagcg
     5401 accggagtta gcgatagaac catcctcttc agcaaagcag gtcgaaggca gacggaatac
     5461 ttcagtctga atagacgccg gatcgacatc gttcgactcg ccgtggttct gccagaaggt
     5521 agaggtttca gtcaccagcg gatcgataac caccatgtac ttcagtttgc tcaggcagct
     5581 caccactttg tttttatccg ggaaggacgc aaccgggtta aagccctggc agaaataacc
     5641 ggtaactttg ccttcatcca tcatgttgaa atacttgatg acgtcgtagg tctggtccca
     5701 cttcggcagc cagtcatagc cccagttgtt ctctttctgc gcggcatcgc catagaaaga
     5761 tttcatcagg ctaacgaaga acttcggata gttgctccag tagttcacct gatcagccag
     5821 cgtcgctttc ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt tttctgacgg
     5881 cagcgtcaga taacctggca ggctggtaga gagcagacct aagtcagtca gcccctgaat
     5941 gttggagtga ccacgcaatg cgttcacgcc gccaccggcc atacccatgt taccgagcag
     6001 caactggatc atcgccatag tacggatgtt ctgcgcaccc acggtgtgct gcgtccagcc
     6061 cagcgcgtac aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca gcacttcaca
     6121 cactttcagg aagtcggctt ttggcgtacc acagatgttt tcaacaacgt ccggcgtgta
     6181 gcgggaaacg tgcgctttca gcaggttcca cacacagcgc ggatgagtca gtgtttcatc
     6241 gcgtttcgca tagccgtttt catcgaactg atagttccag gacgatttgt cgtactggcg
     6301 tttttcagcg tcataaccgc tgaacaggcc atcttcgaaa gcaaaatcat cacgcaccag
     6361 taggctggcg ttggtgtaat gtttaacgta ttcggcgttg attttgttgt tttcgatcag
     6421 gtagcgcaaa atgccagaca ggaacgtaat gtccgtaccg gaacgaatag gtgcgtaaat
     6481 atccgccaca gaagcggtac gcgtaaaacg gggatcgaca acgatcaagg ttgcatcgtt
     6541 gttgtttttc gcttccatcg cccagcggaa accgacggga tgcgcttcag cagcgttacc
     6601 gcccattacc attacgacgt tagcgttttt gatatccacc cagtggttgg tcatagcacc
     6661 gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacgc gcgcctggtt
     6721 gtctaccgcc agcatcccga gggagcgggc aaatttctgg gtcagcatcc cggtttcgtt
     6781 gctggcaccg gaggcacaca gcataccggt agaaagccaa cggtttaccg ttacgccctg
     6841 ctcgttcttt tcaataaagt tagcgtcacg gtcagctttc atcagcttcg caatacggga
     6901 gaatgcttct tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac gatatttcgg
     6961 gtagcgcaga cggttttcac tgttgacgta atccagcaaa ccggcccctt tcgggcacag
     7021 cgcaccacgg cttaccggat gatccgggtc accttcaatg tgataaatcg cttctctggc
     7081 gttttttgcg ccatcaccca ggctatacat caatagcccg caacctacgg aacagtatgt
     7141 gcaggtgtta cggatctctt tagcgcgtaa taatttgtag tttcgcgcct gagccagtgc
     7201 ttgcttcggg gcaaagccca atgccgctac cgttgttcca gccataccgc ccgcgcagat
     7261 tttaaaaaat tgtctgcgac tgacgtccat tgctttcctc ttttttcagg ggtattactg
     7321 cgcgaggaaa ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa agaacacgtg
     7381 tttgccacaa aataatccgc ctgcgcaaat tgacctacct caatagcggt agaaaaacgc
     7441 accactgcct gacaggccag ttaaaaaaat gctataaaat tcagcttaat ttttaacggc
     7501 aagagagaca aaacagcgag catgacacga caaaaagcaa cgctcatagg gctaatagcg
     7561 atcgtcctgt ggagcacgat ggtaggattg attcgcggtg tcagcgaggg gctcggcccg
     7621 gtcggcggcg cagctgctat ctattcatta agcgggctgc tgttaatctt cacggttgga
     7681 tttccgcgta ttcggcagat tccgaaaggc tatttactcg ccgggagtct gttattcgtc
     7741 agctatgaaa tctatctggc gctttcctta gggtatgcgg cgacccgtca tcaggcgatt
     7801 gaagtgggta tggtgaacta tctgtggccc agcctgacaa ttctctttgc cattctgttt
     7861 aatggtcaga aaaccaactg gctgattgta ccaggattat tattagccct ggtcggcgtc
     7921 tgctgggtgt taggcggtga gaatgggtta cattatgatg aaatcatcaa taatatcacc
     7981 accagcccat tgagttattt cctggcgttt attggtgcgt ttatctgggc agcctattgc
     8041 acagtaacga ataaatacgc acgcggattt aacggaatta ccgtttttgt cctgctaacg
     8101 ggagcatgtc tgtggattta ctattttctc acgccacaac cagaaatggt atttagcacg
     8161 cccgtcatga ttaaactcat ctctgcggca tttaccttag gatttgctta tactgcatgg
     8221 aatgtcggta tattgcatgg caatgtcacc ataatggcgg taggctcgta ttttacgcct
     8281 gtactttcct cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt ctcgttctgg
     8341 caaggcgcac taatggtctg cggcggttcc ctgctctgct ggctggcgac acgtcgtggt
     8401 taaaataatt gccgggtcta cggcccggca gttaatcaga taaattccga caatattaaa
     8461 aaataaattt caaataccag taattcacct tatatctcat ttcgacttcg atcacatatc
     8521 gataaataat attattctat ttttctcatt gtttttaatc accattctat tttaaaaaca
     8581 gtatgtcatt tataaatgac gccgtttaaa attcgtttag aaaatacgtt taacgataat
     8641 taaaaggatt ctgttaaatg aaattaaaaa tagttgcggt ggttgtaact ggtttgttag
     8701 ctgcgaacgt agcacacgct gccgaagtct ataacaagga tggtaataaa ctcgaccttt
     8761 atggcaaggt taccgctcta cgttatttta ctgatgataa gcgtgacgat ggtgataaaa
     8821 cttatgcccg tctcggcttt aaaggagaaa cgcaaatcaa tgatcaaatg attggttttg
     8881 gtcactggga atatgatttt aaaggctata acgatgaagc caacggctcg cgcggcaaca
     8941 agacccgtct ggcctatgct ggtttaaaaa ttagtgaatt tggctctctg gactatggtc
     9001 gtaactacgg tgtcggctac gacattggtt catggaccga tatgttgcca gaatttggtg
     9061 gcgatacctg gagtcagaaa gatgtcttca tgacataccg taccaccggt ctagcaacct
     9121 atcgcaacta cgatttcttt ggcttaattg aagggctgaa ctttgccgcg caatatcaag
     9181 gcaaaaataa acgtactgac aacagtcatc tttatggtgc tgacgacacg cgtgccaacg
     9241 gtgacggttt cggtatctcc tcaacttatg tttatgatgg ctttggtatc ggtgcggtat
     9301 ataccaaatc cgatcggaca aatgcgcagg aaagagccgc agctaatcct ctcaatgcct
     9361 ccggtaagaa tgcagaacta tgggccacag gtataaagta tgatgccaac aacatctact
     9421 ttgcagctaa ttacgatgaa acattaaaca tgaccaccta tggggatggt tatatctcta
     9481 acaaagcaca aagttttgaa gtggtggcgc aatatcaatt cgacttcggc ttgcgcccat
     9541 cactcgctta cctgaaatcg aaaggcagag atctgggccg ctacgccgac caggacatga
     9601 ttgagtatat cgacgttggt gcgacgtatt tcttcaacaa aaatatgtcg acctatgttg
     9661 attataaaat caacctgatt gatgaaagcg actttacccg tgccgtagat attcgcaccg
     9721 ataacatcgt cgctacgggc attacctatc agttctaacg tcctctcctg accagccctt
     9781 ctctgggctg gtttttgatt taagtgctgc tgtatgactc cttaagtaaa aatgagcaat
     9841 tcttgatatt tctggtcatt cacctcaatt aatattattc catatcctcc ggggacatct
     9901 tactataata tttattgatg agtgattatg accaatatta atacagcttg tgtaaaaaat
     9961 aatgccagtt atcaacttaa taacgcatta cctaacaaag aaaccatctc cagcaatttt
    10021 tgtgaacgac tagaacaatg gggtaataaa tcggttaata atggtgaaga aagagcaatt
    10081 gccgtggagc gaattaaaga agcttacaat tcgaacatgg catctcttga tttgtcatat
    10141 cttgatttga gtgaattacc gcctatcccc tcgacagtta atacgttaaa tctggaaaac
    10201 aattgtctaa cttgtcttga cttagctgac aatgccagtc tcgttaatat caacctcagc
    10261 gttaataaaa ttaacacgat aacctttcca aaagaaccaa aactggaaaa catttatatt
    10321 aatcataaca atctggaaag tttagattta aaaaatcagc attcattggt taatctggaa
    10381 gcgcaaaata atagcctaaa aaaatgatat ttctgatagt tataaactga aatttcttaa
    10441 tcttgattat aacaagctag catcgctgga tgtctcccgg caagaatctc tgattgagtt
    10501 aagtgcccat cacaatatga tcactgacct tatattacac aatcatccca ggatgaaaac
    10561 aatcacttta aacgacaacc atattgcaca tttaaacgcc aaaaacacta caaaactgga
    10621 atatttaaac ttaagcaata acaatttact gccaaccaat gacattgatc aactaatatc
    10681 atcaaaacat ctttggcatg tattagttaa cggcatcaac aatgatccac ttgcacaaat
    10741 gcagtactgg actgcagtaa gaaatataat tgatgacact aatgaagtga ccattgattt
    10801 atcaggactt aatttaacca ctcaaccacc agggctgcaa aacttcacct ctatcaatct
    10861 tgataataac caactcacac attttgatgc aaccaactac gatagactcg taaagctaag
    10921 tctgaatagt aatgctcttg agtcaataaa ttttcctcaa ggcagaaatg taagtattac
    10981 acatatatct atgaataata atgctctcag aaatattgat atagataggc tttcatcagt
    11041 tacttatttt agtgcggcac ataatcaact agagtttgtg caattagaat cttgcgaatg
    11101 gctgcaatac ctgaatctca gccataatca attaactgat attgttgcag gtaataaaaa
    11161 tgaactctta ctgctggatc tatcccataa taaactaaca agtttacaca atgtcttatt
    11221 tcccaacttg aatacgttac ttatt
//
LOCUS       DAJTYH010000118        11178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTYH010000118 DAJTYH010000000
VERSION     DAJTYH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(205..280)
                     /locus_tag="OO609_003313"
     tRNA            complement(205..280)
                     /locus_tag="OO609_003313"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Ile,seq:cat)
     gene            839..3091
                     /locus_tag="OO609_003314"
     CDS             839..3091
                     /locus_tag="OO609_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634618.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3427..4359
                     /gene="csiD"
                     /locus_tag="OO609_003315"
     CDS             3427..4359
                     /gene="csiD"
                     /locus_tag="OO609_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6634619.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4379..5647
                     /gene="lhgO"
                     /locus_tag="OO609_003316"
     CDS             4379..5647
                     /gene="lhgO"
                     /locus_tag="OO609_003316"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6634620.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5670..7118
                     /gene="gabD"
                     /locus_tag="OO609_003317"
                     /pseudo
     CDS             5670..7118
                     /gene="gabD"
                     /locus_tag="OO609_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7126..8412
                     /gene="gabT"
                     /locus_tag="OO609_003318"
                     /pseudo
     CDS             7126..8412
                     /gene="gabT"
                     /locus_tag="OO609_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8650..10050
                     /gene="gabP"
                     /locus_tag="OO609_003319"
     CDS             8650..10050
                     /gene="gabP"
                     /locus_tag="OO609_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6634621.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10071..10733
                     /gene="csiR"
                     /locus_tag="OO609_003320"
     CDS             10071..10733
                     /gene="csiR"
                     /locus_tag="OO609_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6634622.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10734..>11178)
                     /gene="kbp"
                     /locus_tag="OO609_003321"
     CDS             complement(10734..>11178)
                     /gene="kbp"
                     /locus_tag="OO609_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6634623.1"
                     /translation="LFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDADKV
                     NIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVKSG
                     DTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
BASE COUNT         2861 a         2746 c         2792 g         2779 t
ORIGIN      
        1 gcatagcggg cattttttcc aggtactttt gaatgagtac tgatgaataa atacattgca
       61 gtggcgtgcc acgtaccaaa acaccagccc tcattcgaaa ccacccaccg cacttcttcc
      121 ttgaaatggc gttagtcatg aaatatagac cgccatcgag taccccttgt acccttaact
      181 cttcctgata catgaatgat gatttggtgg cccttgctgg acttgaacca gcgaccaagc
      241 gattatgagt cgcctgctct aaccactgag ctaaagggcc ttaagtatgc aataacaata
      301 cttataaacc acgcaataaa catgatgatc ttatgatgta aataacagat ttttatgcgt
      361 tcccattcac cttgggtcgt gattacacgc gacataaaac ccgacaccgc ctccattcgc
      421 aaagtcgata ctcgcagtca acaagcaaat gttaataatt agcactatct atagttatca
      481 tcgattcaat gatactttgt aatgattttg tatctaataa tataacttta ttacattagc
      541 tgaagagttt tcgcatcatt atgatatctg ttacttttca ctccataaaa ataaacttca
      601 tatagcaata tattctttca tagatcttat tctgctaatc attagtttcg tatgcgcgat
      661 ttttgacagt tgcctctcca gaccacatcg ataattaaaa aacagattta aacattatcc
      721 ttttccatat agatattgga taaaaatagg acatctgttt gcaattactt tcacaacaat
      781 taaacatttt tatgttttcg tatacatcat attactctac cattagagga acgttattat
      841 gttttctata aaaccaggac ctggaaattt acctatcgac aaccccacat tgttatcatg
      901 gaacattact gatggggatc taaattccaa atttaataca ttagaatatc taaactgtat
      961 aacaaatatt attaatgctt gtggagttta tcctcaggat ttaaaagaca gagaaattat
     1021 atcaactttt cacgcagaaa aagtcattaa tgatctgtta aaaaacgatt ataaaatttc
     1081 cctttctcca gatacaactt accgagagct gaataaagct gcacagagta gcattacagc
     1141 gccagacagg ataggagaag ggaaaacatg ggtttatcaa cgagatacaa tggttgaaag
     1201 aggtgataac agtggtgttc atcagtatgg tccagctgaa catttcactc acattatatc
     1261 tgacaaaccg tccccaaaag ataagtatgt tgcatatgct attaacattc ctgactatga
     1321 gctagcagcc gatgtatata atattaacgt gacgtcacct tccggacagc aagaaacatt
     1381 taaaatacta atcaatccag aacatctacg gcaaacactt gagcgtaaat ctcttactgc
     1441 tgttcagaaa tcacaatgtg aaatcatcac ccccaaaaaa cctggcgaag cgattcttca
     1501 tgcttttaat gccacctacc agcaaatcag ggaaaatatg tctgagtttg cacgttccca
     1561 ttatgggtat atacaaatcc ctccagtgac aactttccgc gccgacggac cagaaactcc
     1621 cgaagaagaa aaaggttact ggtttcacgc ttatcaaccc gaagatcttt gtaccatcca
     1681 taatccaatg ggggatttgc aggattttat cgcattggtt aaagatgcta aaaaatttgg
     1741 tatcgatatc attcctgatt atacctttaa ctttatggga atcgggggaa gtggtaaaaa
     1801 tgacctggat tatccctctg ctgatatacg agcgaagatc agtaaagata tagaaagtgg
     1861 tatccctggc tattggcaag gtcaggtttt gattccattc attaaagatc cagtaacaaa
     1921 agaacgtaaa caaatccatc cagaagatat acatctcact gcaaaagact tcgaagcaag
     1981 taaagataac atctctaagg atgaatggaa aaacctccat gcattaaaag aaaagagttt
     2041 aaatggaatg cctaaaacaa cccccaaaag tgaccaggtt attatgttgc aaaatcaata
     2101 cgttcgtgaa atgcgaaaat atggcgtacg aggtttacgt tatgatgcag caaaacactc
     2161 aaaacatgaa caaatagaaa gatcaataac cccaccgctt aaaaattata atgagcgatt
     2221 acacaatact aacttattta acccaaaata tcatgaaaaa accgttatga attacatgga
     2281 atatctggta acttgtcagt tgaatgaaga acaaatgtca tcgctgcttt atgaaagaga
     2341 tgatttaagc gccattgatt tttcattgct catgaagacg ataaaagcct tttcattcgg
     2401 gggagatctc ctaacccttg catcaaaacc gggttccaca atctcaagca tcccatcaga
     2461 aagacggata ttgattaaca ttaaccacga ttttcctaac aatggcaatc ttttcaatga
     2521 ctttctattt aaccatcaac aagatgaaca attagcaatg gcatatatgg ccgctctccc
     2581 gttcagcagg cctttagttt actgggatgg ccaggtatta aaatcaacga ctgaaattaa
     2641 aaattatgat gggcccacgc gtgtcggtgg tgaggcgtgg cttaataaag gttgctctac
     2701 ctatcagcag ctctacaatg aattccacgc attatatata gataaagcag gaatatggag
     2761 cgcatttgag ggtgtatttg caactaaaaa cgttctggcc tttagtcgtg gggattctgt
     2821 gaacattaat cactctcctc atgatggact agttataata aataaaggaa acgaagaagt
     2881 tgaaggtacc tggcctaaca aattgcaacc tggaatatat aaaaacatgg ggagtaatag
     2941 cgttaacatt attattaata atacccgaaa aattatcccc ccttgtaaag tatttacgct
     3001 tagaggcgga tctctaaata tcaatattcc taggcgtagc gctcttcttt tagggaaaac
     3061 aggagaaccg ccgaactatc tctatttata atttaatgtt atatctgccc cgataaaacg
     3121 gggcagataa tatgtttagt ttactaacgg tcattttgta gtgaagccat ttactgtttt
     3181 ttatcgacca gataatctgt tctctaatgt taactccccc taacctgttg ctttagttat
     3241 tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat ttgttgcttt
     3301 tgatcacaat aagaaagcaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt
     3361 tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg
     3421 cttctgatga atgcactgac cgccgtacaa aataacgctg tcgattcagg ccaggactat
     3481 agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc
     3541 gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca ggcgctggag
     3601 tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag
     3661 ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat
     3721 gcggtaggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta
     3781 gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc
     3841 gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa
     3901 ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc
     3961 gatgactggg aacatctgga ccactatttc cgccatccac tggcgcgtcg cccgatgcgc
     4021 tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt tccatccagt gttcgacgtt
     4081 gatcaacagg gtcgcccggt gatgcgctat atcgaccagt tcgtccagcc aaaagatttt
     4141 gaagaaggcg tgtggttgag cgagctttcc gatgccattg aaaccagcaa aggcattctt
     4201 tctgtacccg ttcccgttgg caaattcctg ttgataaaca acctgttctg gctgcacggt
     4261 cgcgaccgct ttactccgca cccggatctg cgccgtgaac tgatgcgtca gcgtggctat
     4321 ttcgcttacg ccactcacca ctaccagacg catcagtaag cgcaaaggaa ttgagcggat
     4381 gtatgatttt gtgattattg gcggcggcat catcggcatg tcgaccgcca tgcaactgat
     4441 tgatgtttat ccggatgccc gcattgcgtt gctggaaaaa gagtccggcc cggcctgtca
     4501 ccagacgggc cacaacagcg gcgtgatcca tgccggggtc tattacacgc ccggcagcct
     4561 gaaggcgcag ttttgcctgg cggggaaccg tgccaccaaa gccttttgcg atcaaaacgg
     4621 cattcgctat gacaactgcg gcaagatgct ggtcgccacg tctcagctcg aaatggaacg
     4681 gatgcgcgcc ttatgggaac gcacagcggc gaacggtatc gagcgcgagt ggataaacgc
     4741 cgtggaactg cgcgagcgcg aaccgaatat caccgggctt ggtggcattt ttgtgccgtc
     4801 cagcggcatt gtcagctacc gcgaagtgac ggcggcaatg gcaaaaatct tccaggccag
     4861 aggcggcgag attatttata acgccgaagt cagcgccctc agtgagcata aaaacggcgt
     4921 ggtgatacgc acccgtcagg gcggtgaata tgaagcatcg acgctgatta gctgttccgg
     4981 gctgatggct gaccggctgg tgaaaatgct cggcctcgaa ccgggcttta ttatctgccc
     5041 gttccgtggc gagtatttcc gccttgcgcc ggagcataac cagattgtta accacctgat
     5101 ttaccccatt cccgaccccg cgatgccatt tttgggcgtg catctcaccc gcatgatcga
     5161 cggcagcgtg accgtcgggc caaacgcggt gctggctttc aaacgcgaag gctatcgcaa
     5221 gcgcgacttc tcatttagtg acacgctgga gattttgagc tcgtcgggga ttcgccgggt
     5281 gctgcaaaac catctacgct caggactggg cgagatgaaa aactcgctgt gcaaaagcgg
     5341 ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg ttaagcgatc tccagccctg
     5401 gcccgccggt gtgcgggcgc aggcagtatc gccggacggc aagctgattg acgattttct
     5461 gtttgtcacc accccacgca cgatccacac ctgcaatgcg ccctccccgg cagcgacatc
     5521 agcaattcct attggtgcgc atattgtcag caaggtacaa acgctgttgg caagccagag
     5581 taaccccgga cgcacgctgc gagcggcacg tagtgtggat gccttacacg ccgcatttaa
     5641 tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa cgacagtaac ttattccgcc
     5701 agcaggcgtt gattaacggg gaatggctgg acgccaacaa tggtgaagtc atcgacgtca
     5761 ccaatccggc gaacggcgac aagctgggta gcgtgccgaa aatgggcgca gatgaaaccc
     5821 gcgccgctat cgacgccgcc aaccgcgccc tgcccgcctg gcgtgcgctc accgccaaag
     5881 aacgcgccac cattctgcgc aactggttca atttgatgat gcagcatcag gacgatttag
     5941 cgcgcctgat gaccctcgaa cagggtaaac cgctggctga agcgaaaggc gaaatcagct
     6001 acgccgcctc cttcattgag tggtttgctg aagaaggcaa acgtatttat ggcgacacca
     6061 ttcctggtca tcaggccgat aagcgcctga ttgttatcaa gcagccgatt ggcgtcaccg
     6121 ccgccatcac gccgtggaac tttccggcgg cgatgattac ccgtaaagcc ggtccggcgc
     6181 tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca gacgccgttc tctgcgctgg
     6241 cgctggcgga actggcgatt cgcgcgggca ttccggctgg cgtgtttaac gtggtcaccg
     6301 gttcggcagg cgtggtcggt aatgaactga ccagcaaccc gctggtacgc aaactgtcgt
     6361 ttaccggtta gaccgagatt ggccgccagt taatggaaca gtgcgcgaaa gacatcaaaa
     6421 aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt ctttgacgat gccgatctcg
     6481 ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa cgccgggcaa acctgcgtct
     6541 gcgccaaccg tttatacgtg caggacggcg tgtatgaccg ctttgccgaa aaattacagc
     6601 aggcggggag caaactgcac atcggcgacg ggctggagaa aggcgtcacc atcgggccgc
     6661 tgatcgatga aaaagcggta gcaaaagtgg aagagcatat tgccgatgcg ctggagaaag
     6721 gtgcgcgtgt ggtttgcggc ggtaaagcgc acgaacgcgg cggcaacttc ttccagccaa
     6781 ccattctggt ggacgttccg gccaacgcca aagtgtcgaa agaagagacg ttcggccccc
     6841 tcgccccact gttccgtttt aaagatgaag ccgatgtgat tgcacaagcc aatgacaccg
     6901 agtttggtct tgccgcctat ttctacgccc gtgatttaag ccgcgtcttc cgcgtcggcg
     6961 aggcgctgga gtacggcatc gtcggcatca ataccggcat tatttccaat gaagtggccc
     7021 cattcggcgg catcaaagcc tcgggtctgg gtcgtgaagg ttcgaagtat ggcatcgaag
     7081 attacttaga aatcaaatat atgtgcatcg gtctttaact ggagaatgcg aaggaacagc
     7141 aataaagagt taatgcagcg ccgcagtcag gcgattcccc gtggcgttgg gcaaattcac
     7201 ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg acgtttaagg ccgtgagtat
     7261 cttgatttcg cgggcgggat tgcggtgctc aataccgggc acctgcatcc gaaggtggtg
     7321 gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct gcttccaggt gctggcttac
     7381 gagccgtatc tggagctgtg cgagattatg aatcagaagg tgccgggcga tttcgccaag
     7441 aaaacgctgc tggttacgac cggttccgaa gcggtggaaa acgcggtaaa aatcgcccgc
     7501 gccgccacca aacgtagcgg caccatcgct tttagcggcg agtatcacgg gcgcacgcat
     7561 tacacgctgg cgctgaccgg caaggtgaat ccgtactctg cgggcatggg gctgatgccg
     7621 ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg gcataagcga ggatgacgct
     7681 atcgccagca tccaccggat cttcaaaaat gatgccgcgc cggaagatat cgccgccatc
     7741 atgattgagc cggttcaggg cgaaggcggt ttctacgccg cgtcgccagc ctttatgcag
     7801 cgtttacgcg ctctgtgtga cgagcacggg atcatgctga ttgccgatga agtgcagagc
     7861 ggcgcggggc gtaccggcac gctgtttgcg atggagcaga tgggcgtggc gccggatctt
     7921 accacctttg cgaaatcgat cgcaggcggt ttcccgctgg cgggcgtcac cgggcgcgcg
     7981 gaagtgatgg acgctgttgc accgggcggt ctgggcggca cctatgccgg taacccgatt
     8041 gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc aggaaaatct gctgcaaaaa
     8101 gccaacgatc tggggcagaa gctaaaagac ggattgttgg cgatagccga aaaacacccg
     8161 gagatcggcg acgtacgcgg gctgggatcg atgattgcca tcgagctgtt tgaagatggc
     8221 gatcacagca aaccggacgc aaaactcacc gccgagatcg tggcacgcgc ccgcgataag
     8281 ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct tgtaccactc
     8341 accattgaag acgctcagat ccgtcagggt ctggagatta tcggtcagtg ttttgatgag
     8401 gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg cgcgtcttgt ccggcctacg
     8461 agaatccaag tcggataagg cgtttacgcc gcatccgaca atctgtacgt gaacaggaat
     8521 aaatctatgt cggccggata aggcggagcc gctctccggc aaaaagaatc aataacaatt
     8581 aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga
     8641 ggattcagga tggggcaatc atcgcaacca cacgagttag gcggcgggct gaaatcacgc
     8701 cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc
     8761 gtcgccatcg ccgaagcgga cccggcggta ttactggcct atctgttcgc cggattactg
     8821 gtggttatga ttatgcggat gctggcggaa atggcagttg ccacgcccga taccggttcg
     8881 ttttccacct atgccgataa agccattggc cgctgggcgg gctataccat cggctggttg
     8941 tactggtggt tttgggtact ggttatccca ctggaagcca acatcgccgc catgatcctg
     9001 cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac cctcgcctta
     9061 actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg
     9121 tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt
     9181 tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg ctttatgccc
     9241 aacggtttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca
     9301 gaaattgtca ccattgccgc cgcggaatcc gacatgccgg aaaaacatat tgtccgcgcc
     9361 accaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtcgtg
     9421 gcattaatac cgtggaatat gccggggctg aaagccgttg gatcttatcg ctcggttctg
     9481 gaattgctca atattcccca tgcgaaatta atcatggact gtgtgatatt actttccgta
     9541 accagttgcc tgaactcggc cctgtatacc gcctcaagga tgctctactc cttaagccgt
     9601 cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg
     9661 gtgttactct ccaccggcgc agcattctta acggtggtgg tgaactatta cgcgcctgcg
     9721 aaggtattta aatttctgat cgacagctcc ggcgctatcg ccctgctggt ttatttagtc
     9781 atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc
     9841 ttgcgaatgt ggcttgatcc ctggctcacc tggctggtca tcggctttat tacctttgtg
     9901 ttggtagtga tgctattccg tccggcgcaa cagttagaag tgatctccac cggcttatta
     9961 gcgataggga ttatctgtac cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg
    10021 caaaaaacac ccgttcataa tacgcgctga taatgatcag gagtcacacc atgaccatta
    10081 cgtctctgga tggctatcgc tggctgaaga acgatattat tcgcggtaat tttcaaccgg
    10141 atgaaaaatt acggatgagt ttactgacat cgcgttatgc acttggcgtt gggccgttgc
    10201 aggaagcact ttctcaactg gtggcggaac ggctggtcac ggtggtgaat caaaaagggt
    10261 atcgggtggc gtctatgtca gagcaggagc tgctcgatat tttcgacgcc cgcgccaata
    10321 tggaagcgat gctggtcagt ctggcgattg cccgcggtgg cgatgaatgg gaggcagacg
    10381 ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg tgacgccagc gagaaaatgc
    10441 ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc aattgtggcg ggctgtggtt
    10501 ctcactattt gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgt tatcgattta
    10561 tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga ggataaacac gatcagcacc
    10621 agaccctgac tgcggcggta ctgccgcgag atacagcgcg cgccagtgag ttaatgcgcc
    10681 agcatttact gacgccaatt cccattatcc agcaggcgat ggctggcaat taactactct
    10741 tccggaatac gcaacacttg ccccggataa attttatccg ggctttttag catcggttta
    10801 ttcgcttcga agattttatt gtacagatta gcgttaccgt agacctgttt ggaaatggca
    10861 ctcagagtgt cgccagactt aacggtataa aactggctgg cagtggctgg tgtcgccgtt
    10921 ttcacctgat catcaacact ggcaataccg gaaatattcc ccaccgcaac aaggattttc
    10981 tccttcgcct cctgactcag gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga
    11041 atgttcactt tatcggcatc aggtataccg gttttgctca gatgctcctg caccttcttc
    11101 gcctgatcgt ctttatcgtg ctgacctgta accgcatccc agagtttttc tccggcatct
    11161 ttaacaaaat tgaacaga
//
LOCUS       DAJTYH010000119        11107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTYH010000119 DAJTYH010000000
VERSION     DAJTYH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..461)
                     /locus_tag="OO609_003322"
     CDS             complement(<1..461)
                     /locus_tag="OO609_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6634624.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKN"
     gene            complement(573..1601)
                     /gene="malI"
                     /locus_tag="OO609_003323"
     CDS             complement(573..1601)
                     /gene="malI"
                     /locus_tag="OO609_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6634625.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1776..3368
                     /gene="malX"
                     /locus_tag="OO609_003324"
     CDS             1776..3368
                     /gene="malX"
                     /locus_tag="OO609_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6634626.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3378..4550
                     /gene="malY"
                     /locus_tag="OO609_003325"
     CDS             3378..4550
                     /gene="malY"
                     /locus_tag="OO609_003325"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6634627.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4654..5655
                     /gene="add"
                     /locus_tag="OO609_003326"
     CDS             4654..5655
                     /gene="add"
                     /locus_tag="OO609_003326"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6634628.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5691..6731)
                     /locus_tag="OO609_003327"
     CDS             complement(5691..6731)
                     /locus_tag="OO609_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6634629.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6974..7099
                     /gene="blr"
                     /locus_tag="OO609_003328"
     CDS             6974..7099
                     /gene="blr"
                     /locus_tag="OO609_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6634630.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7372..7587
                     /gene="ydgT"
                     /locus_tag="OO609_003329"
     CDS             7372..7587
                     /gene="ydgT"
                     /locus_tag="OO609_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6634631.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7673..8113
                     /locus_tag="OO609_003330"
     CDS             7673..8113
                     /locus_tag="OO609_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6634632.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8190..8771
                     /gene="rsxA"
                     /locus_tag="OO609_003331"
     CDS             8190..8771
                     /gene="rsxA"
                     /locus_tag="OO609_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6634633.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8771..9349
                     /gene="rsxB"
                     /locus_tag="OO609_003332"
     CDS             8771..9349
                     /gene="rsxB"
                     /locus_tag="OO609_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6634634.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9342..>11107
                     /gene="rsxC"
                     /locus_tag="OO609_003333"
     CDS             9342..>11107
                     /gene="rsxC"
                     /locus_tag="OO609_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6634635.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2586 a         2864 c         2804 g         2853 t
ORIGIN      
        1 atatttttat tttctgccgc catagaagtg atggtcagaa taacgccacc tccatttttt
       61 tccatttctg gcgcaacaag ttgtgacaga tggaaaaaag aaaacacatt cagttcataa
      121 gcacggcgaa aatctgccat tggcatatca aacggtttag gaccaccgcc accggcgttg
      181 ttaaccagga tatcaacttt acccagatta ctgacggcaa agtctgccag tgcagagagt
      241 tcctgttcgg aagtaatatc acaacgccag gcaaatgcct gaccatccag ttgttgaatt
      301 tcgtctacaa catggttagc tgcgtcggcg ttaatatcac tgaccaccac agatgcgcca
      361 gctgtcgcga atgtaatggc gatttcttta ccaatacctg cacccgcacc tgtgatgatg
      421 gcgcattttc cgtcgagtct caggttgtca gaattaaaca cataaacctc ctgtgaactt
      481 cagtaaaaat aaaaggcacc gcctgtgtgc cacatttttt atcattaacc ttgaggaaag
      541 gacactacca agtatagcag ataaagagag gattatttcg ccgcaatgag ccgggcggga
      601 ataataagat tgcgtgaatg cgtctcttca tgggtgattt tttgcatcat gcgatccgca
      661 agtgtgatac caagttcccg cgctggcgtg ctggcccagg taacaggtat atcatcaagt
      721 gtggttggtg tcgcatcggt aaatgccgcc agcgaaacct gttgctcaaa gtaacgatcg
      781 actccgcttt ccccgctttg ccgccctgct ttcagcaaac caaaccatgc ccccatcgca
      841 atagtttcgt tatagcaaac cacggcactg atggtcgggt tatgacgtaa aagcgccgtg
      901 atagcttccg cggcttgctt ctggctggaa gtgcactcca acacccaatc gctgtgaaac
      961 ggcaggccaa attttagtag agttgcacaa tagcccccca cccgttctgc acgggttaat
     1021 gaggaacttt gccctcccag ccaggcgatc cgctgatgcc cattgcgaat gagatgctcc
     1081 gtcaacaact gtgcagcctg catgttgtcc gggcgaaccg tatcaacatc atcaagataa
     1141 ctggcacggg aagcgaaaat caccgggata gctttttctt ctgccatccg ccgcaggtca
     1201 tcgctgcttc ctgcagcccc ggcaattacc acaccatcga caccctgatt cagtaacagt
     1261 gaaaaccgct gtgccagctg ctcgccgtct ttaccgccgt gaagcaaaaa aaccatccgt
     1321 ccctgtgctt ccagagcttc cgtcaatccg gccgtcaatt cggcgtaaaa cggcgcagat
     1381 aaatcacgga cgatcaaacc aatgacgccg ctttgcccgc cgcgcagcgc cgacgcctgg
     1441 cgattgcgca caaatcccag ctcttcaatg gcggcgttaa cgcgttctcc tgtggcggta
     1501 gagattcgcc ctttgccatt aagcaccagc gaaacggtac ttaccgacac gcccgcagcc
     1561 agcgcaacat catgaatggt tatttttttg gcggtagcca tgcagatgac ctactccctg
     1621 attatgtgac agataaaacg ttttaccttt tatttatctt atacccgcta ttatcgttgc
     1681 gtaatgtgat ttatgactca ctaaaatttg ataaaacgtt ttatcttctc gcgcaattta
     1741 ctgaatccag attgttctct acgaggagtc gttttatgac ggcgaaaaca gcaccgaaag
     1801 tcacgctgtg ggagttcttc cagcagttag gcaaaacctt catgttaccc gtggcattat
     1861 tgtcgttctg cggcattatg ctcggcattg gtagttctct tagcagccat gatgtcataa
     1921 ccctgatccc ggtcctgggc aaccccgtgt tgcaggctat ctttacctgg atgagtaaga
     1981 ttggctcgtt tgcttttagt ttcctgcctg tcatgttctg tatcgccatc ccgctgggcc
     2041 tggcacgcga aaataaaggc gtagcggcat tcgctggctt cgtcggttat gcggtaatga
     2101 acctcgcggt aaacttctgg ttgaccaata aaggcattct gccaaccaca gatgccgccg
     2161 ttctgaaagc caataacatc cagagcattc ttgggatcca gtcgatcgat accgggatcc
     2221 tcggtgcggt gatcgccggt attatcgtct ggatgctgca tgagcgtttc cataatatcc
     2281 gcctgccgga tgcgctggca ttcttcggcg gtacgcgctt cgtaccaatt atctcctcgc
     2341 tggtgatggg ccttgtcggc ctggtgattc cattagtctg gccgattttc gccatgggta
     2401 ttagcggctt gggccatatg ataaacagcg cgggtgattt cggaccgatg ctgtttggta
     2461 ccggtgaacg tctgctgttg ccgtttggtc tgcatcacat tctggtggca ttaattcgct
     2521 ttaccgacgc aggcggcacg caggaggtct gcggtcaaac cgtcagcggc gcactgacca
     2581 tcttccaggc gcaattgagt tgcccgacca ctcacggttt ttctgaaagc gtcacgcgtt
     2641 tcctttcgca aggtaaaatg cctgcgtttc tcggcggtct accaggtgca gcgttagcta
     2701 tgtatcactg cgcgcgcccg gaaaatcgcc ataaaattaa aggtctgctg atttctggcc
     2761 tgatcgcctg cgtcgttggc ggcactaccg aaccgctgga attcctgttc ctgttcgtag
     2821 cgccagttct gtatgtcatc cacgcgctgt taaccggcct cggcttcacc gtcatgtctg
     2881 tgctcggcgt caccatcggt aataccgacg gcaatatcat cgacttcgtg gtgttcggta
     2941 ttttgcatgg tctgtcaacc aagtggtaca tggtgccagt ggtggcggca atctggtttg
     3001 tcgtttacta cgtcatcttc cgtttcgcta tcacccgctt caatctgaaa accccggggc
     3061 gcgatagcga agttgccagc tcaatcgaaa aagccgttgc cggtgcgccg ggtaaatcag
     3121 gttacaacgt tcctgcaatc ctcgaagcat taggcggtgc cgacaatatt gtcagcctcg
     3181 ataactgcat tacccgtctg cgtttgtctg tggaagatat gtcgcttgtt aatgtgcagg
     3241 cactgaagga caatcgggca attggcgtag tacaacttaa tcaacataac ctgcaggttg
     3301 ttatcgggcc acaagttcag tcagtaaaag atgaaatggc cggtctgatg catactgtcc
     3361 aggcataagg ataagatatg ttcgattttt caaaggtcgt ggatcgtcat ggcacatggt
     3421 gtacacagtg ggattatgtc gctgaccgtt tcggcactgc tgacctgtta ccgttcacga
     3481 tttcagacat ggattttgcc actgccccct gcattatcga ggcgctgaat cagcgcctga
     3541 tgcacggcgt atttggctac agccgctgga aaaacgatga gtttctcgcg gctattgccc
     3601 actggttttc cacccagcat tacaccgcaa ttgatacgca gacggtggtg tatggccctt
     3661 ctgtcatcta tatggtttca gaactgattc gtcagtggtc tgaaacaggt gaaggcgtgg
     3721 tgatccacac gcccgcctat gacgcatttt acaaggccat tgaaggtaac cagcgcacag
     3781 taatgcccgt tgctttagag aagcaggctg acggctggtt ttgcgatatg ggcaagctgg
     3841 aagccgtttt ggcgaaacca gaatgtaaaa ttatgctcct gtgtaaccca cagaatccta
     3901 ccgggaaagt gtggacgtgc gatgagctgg agatcatggc tgacctgtgc gagcgtcatg
     3961 atgtgcgggt tatttccgat gaaatccata tggatatggt ctggggcgag cagccgcata
     4021 ttccctggag taatgtggcg cggggagact gggcgttgct gacatcggga tcgaaaagtt
     4081 tcaatattcc cgcgctgacc ggtgcttacg ggattataga aaatagcagc agccgtgatg
     4141 cctatttatc ggcactgaaa ggacgtgatg ggctttcttc cccttcggta ctggcgttaa
     4201 ctgcccatat cgccgcctat cagcaaggtg caccgtggct ggatgcctta cgcgtctatc
     4261 tgaaagataa cctgacgtat atctcagata aaatgaacgc cgcgtttcct gaactcaact
     4321 ggcagatccc gcaatccact tatctggcct ggcttgatct acgtccgttg aatattgacg
     4381 acaacgcgtt gcaaaaagcg cttatcgagc aagaaaaagt cgcgatcatg ccggggtata
     4441 cctatggtga agaaggtcgt ggttttgtcc gtctcaatgc cggctgccca cgttcgaaac
     4501 tggaaaaagg tgtggctgga ttaataaacg ccatccgcgc tgttcgctaa ccccaattgc
     4561 gcaacgtaaa aaatcgttgc gcaatcgtgg atttttaccc tgctttgttt ttataatggt
     4621 gcgcactttt atatccagaa aaagagtgcg accatgattg ataccaccct gccattaact
     4681 gatatccatc gccaccttga tggcaacatt cgtccccaga ccattcttga acttggccgc
     4741 cagtataata tctcgcttcc tgcacaatcc cttgaaacac tgattcccca cgttcaggtc
     4801 attgccaacg aacccgatct ggtgagcttt ctgaccaaac ttgactgggg cgttaaagtt
     4861 ctcgcctctc ttgatgcctg ccgccgcgtg gcatttgaaa acattgaaga tgcagcccgt
     4921 aacggcctgc gctatgtcga gctgcgtttt tcaccaggct acatggcaat ggcacataag
     4981 ctgcctgtag cgggtgttgt cgaagcggtg atcgatggcg tacgtgaagg ttgccgcacc
     5041 tttggtgtgc aggcgaagct tatcggcatt atgagccgaa ccttcggcga agccgcctgt
     5101 cagcaagagc tggaggcctt tttagcccac cgtgaccaga ttaccgcact tgatttagct
     5161 ggtgatgaac ttggattccc gggaagtctg ttcctttctc acttcaaccg cgcgcgtgat
     5221 gcgggctggc atattaccgt ccatgcaggc gaagctgccg ggccggaaag catctggcag
     5281 gcgattcgtg aactgggtgc ggagcgtatt ggacatggcg taaaagccat tgaagatcgg
     5341 gcgctgatgg attttctcgc cgagcaacaa attggtattg aatcctgtct gacctccaat
     5401 attcagacca gcaccgtagc agagctggct gcacatccgc tgaaaacgtt ccttgagcat
     5461 ggcattcgtg ccagcattaa cactgacgat cccggcgtac agggagtgga tatcattcac
     5521 gaatataccg ttgccgcgcc agctgctggg ttatcccgtg agcaaatccg ccaggcacag
     5581 attaatggtc tggaaatggc tttcctcagc gcagaggaaa aacgcgcact gcgagaaaaa
     5641 gtcgccgcga agtaacaaaa atggatggtg caaattgcac catccatttt tcacgcaagg
     5701 cacaacgtcg cgcgatgttt ggcggattcg atgcccagct caatcaactc cattacctgg
     5761 attgcctggc ttgccggaac cggattttca ccatcgccat ttaacgcatc acgaatcgcc
     5821 gcatagtaag ccggataatt cccaggcacc gtcaacagcg tttcttcgac acgttcctca
     5881 ccttccacgc gggtaagtac gccatcacgc atatcgtagc cccagtcttc ctgcggcaga
     5941 cgctcgccat ttttcagacg ttcttcctgt ggatcgaggc catatttcac ataactgcct
     6001 cgggatccat gcacgatata ccgtgctgac tcagcggctg ccagcatggt accgtgtaaa
     6061 atgactcgcc gctgtggata ggacaagatg gcgtggaaat aatcggtcga ctgcgctccg
     6121 ggccgtaact gtgccaaatc taccgtcatg ctgaccggta aaccaaatag cgtaatggcc
     6181 tgatcaagaa gatgcggtgc taaatcgtac cagataccgc tgcctgggcc gccctgttca
     6241 cgccaacgat cgcgcacctg cggacggaag cggtcaaaat gagactcaaa gtaagcaact
     6301 tcacccagca cgccttccgc gagcaaacct tttagcgtca agaaatcgct atcccaacgg
     6361 cggttatgga atacagacag cacacgcccc aggctttttg ccagcgcatc cagctctcgc
     6421 gcttgtgaca gtgtcacggt aaagggttta tcaacgacca catgtttacc cgcctcaagc
     6481 gccgctttgg ctaacgggaa atgggtatcg ttgggtgtag gaatgacaat caggtctatg
     6541 ttgggatcgt taaacagatg cttcggctca gagacaaccg taaccgttgg ccagtcggct
     6601 tttacttttg tttcatcact gctggagatt accgccagtt ccagcccggg cgtgcccgca
     6661 atcaggggcg catggaaggt tttgctcgca taaccatacc caatcaaccc aacacggatg
     6721 ttgtcgctca tgtcattgcc tctcattgaa gtatgatggc tatttgacac tatcctttac
     6781 ccacgctcaa cagtttaata acctgccagc aataagggat gttgtttaac ttaagtcaaa
     6841 aaaatagcga attttccaac gacaaaagct aaatatcgca aaaacctcag taaaaatctt
     6901 gctggagcta ttattgctaa gtaacattta ccccctgaag ttaatggatc aatcaagaga
     6961 gatgtgggct gtaatgaatc gtcttattga attaacaggt tggatcgttc ttgtcgtttc
     7021 agtcattctt cttggcgtgg cgagtcacat tgacaactat cagccacctg aacagagtgc
     7081 ttcggtacaa cacaagtaag ctctgcactt gtggagcgac atgctgcccg tccgggtgca
     7141 tgttttcact tgtcggatat taaaccagga atttattatc ttgttcgatg ttgttggtga
     7201 ttgtcaggga tagtaaagcg cgttactctt ttcagagcag tttttcatcc ctgaatattt
     7261 gccatcagcg cattcttatt attgtaataa cacaagcgtt cacttttggt gaaattttat
     7321 catcactaat aatacctacc cctttggtat taattatttt atggaccttt tatgactgtt
     7381 caggactact tattaaaatt tcgcaaaatc agttcactcg aaagtctgga aaaactctac
     7441 gaccatctta attacaccct gacggacgat caggaactga tcaatatgta tcgtgctgcc
     7501 gatcaccgtc gcgcagagct ggtttctggc gggcgtttgt ttgacctcgg ccaggtaccg
     7561 aagtccgtct ggcactatgt ccaataaaga aagtagcgat cggttataaa agccttataa
     7621 taacgcccct aaaattagat ttgcctgttt gcgtcgctat tctggagaac ctatgaccac
     7681 cacaacgcca caacgtattg gaggctggtt gcttggcccg ttggcctggc ttttagtcgc
     7741 tctgttgagt acgacgctgg cgttgttgct gtataccgct gcgttatctt ctccgcaaac
     7801 atttcaaacg ctcggcggac aagcactgac gacacaaatc ttatggggcg tttcttttat
     7861 taccgctatc gccatgtggt attacacact gtggttgacc atcgctttct ttaaacgtcg
     7921 gcgttgtgtt cctaaacact atattatctg gctgcttatt tccgtattgc tggcagtaaa
     7981 agcattcgct ttttcacccg tggaagttgg catagccgtt cgccagttgc tgtttacttt
     8041 gttggcaacc gcattgatcg tcccctattt caaacgctcg tcgcgggtaa aagcgacgtt
     8101 tgtgaatccg taataacctt acagttaacc tgttgtcgcc tgctctggat taacggataa
     8161 taggcggctt ttttatttca ggccgaaaaa tgactgacta cctgttactg tttgtcggaa
     8221 ctgtactggt caataacttt gtactggtca agtttctcgg tctctgtccg tttatggggg
     8281 tttccaaaaa actggaaacc gcgatgggca tggggctggc aacaacgttt gtgatgacgc
     8341 tgacgtctat ttgcgcctgg cttatcgata cgtggatttt gatcccactt aatctgattt
     8401 acctgcgcac cctggcattt attctggtga ttgctgtggt cgtgcagttc accgagatgg
     8461 tggtgcgcaa aaccagcccg gtgctttacc gtctgctggg gatttttttg ccgcttatca
     8521 ccaccaactg tgcagtgctc ggcgtggcgt tgctgaatat caatctcggg cacaatttct
     8581 tgcagtcggc gctgtacggt ttttccgccg ctgtcggttt ctcgctggtg atggtgctct
     8641 tcgccgccat ccgcgaacgc cttgctgtgg ctgatgtccc ggcacctttt cgcggtaatg
     8701 ccattgcgtt aattaccgca ggtcttatgt ctctggcctt tatgggcttt agtggtttgg
     8761 tgaagttgta atgaatgcta tctggattgc cgttgccgcc gtgagcctgc tgggcctggc
     8821 gtttggcgcc attctgggtt atgcctcccg ccgttttgcg gtggaagacg atccggtcgt
     8881 tgagaaaatt gacgaaatct taccgcagag ccagtgtggt cagtgcggtt atcccggctg
     8941 tcgcccctac gcggaagcca tcagctgtaa cggtgaaaaa atcaaccgtt gcgccccagg
     9001 tggcgaagct gtgatgctaa aaattgccga gttgcttaat gtcgagccgc agccgctgga
     9061 tggcgaagcg caagagctaa cgcctgcgcg gatggtggcg gttattgatg aaaataactg
     9121 tattggctgc actaaatgta ttcaggcttg tccggtagac gccatcgttg gcgctacccg
     9181 tgtcatgcat acggtaatga gtgatctctg tacgggctgc aatttatgtg ttgatccgtg
     9241 cccgacgcac tgcatctcgt tgcaaccggt cgcagaaaca cctgactcct ggaaatggga
     9301 tctgaacacc attcccgtgc gtatcattcc cgtggaacac catgcttaag ttattctctg
     9361 cattcagaaa aaataaaatc tgggatttca acggcggcat ccatccaccg gagatgaaaa
     9421 cccagaccaa cggtaccccc ctgcgccagg tactactggc gcagcatttt gttattccac
     9481 tgaaacagca tattggcgct gaaggtgagt tgtgcgttag cgtcggcgat aaagtattgc
     9541 gcggccagcc gcttacccgt ggtcgcggca aaatgctgcc tgttcacgcg cccacctcgg
     9601 gtaccgttac ggctattgcg ccccactcta cggctcatcc ttcagcttta gctgaattaa
     9661 gcgtgattat tgatgccgat ggtgaagact gctggatccc gcgcgacggc tgggtcgatt
     9721 atcgctctcg cagtcgcgaa gagttaatcg agcgcataca tcagtttggt gttgccgggc
     9781 tgggcggtgc cggattcccg acaggcgtta aattgcaggg tggtggagat aagattgaaa
     9841 cgttgattat caacgctgct gagtgcgagc cgtacattac cgccgatgac cgtttgatgc
     9901 aggattgcgc ggctcaggtc gtagagggta ttcgcattct tgcgcatatt ctgcagccac
     9961 gcgaaattct tatcggcatt gaagataaca aaccgcaggc gatttccatg ctgcgcgcgg
    10021 tgctggcgga ctctaacgat atttctctgc gggtgattcc aaccaaatat ccttctggcg
    10081 gtgctaaaca attaacctac attctgaccg ggaagcaggt tccacatggc gggcgttcat
    10141 ccgatatcgg cgtattaatg caaaacgtcg gcactgctta tgcagtgaaa cgtgccgtta
    10201 ttgatggcga gccgattacc gagcgtgttg taaccctgac tggcgaagca atcgctcgcc
    10261 cgggcaacgt ctgggcacgg ctggggacgc cagtgcgtca tttattgaat gatgccggat
    10321 tctgcccctc tgccgatcaa atggtgatta tgggtggccc gctaatgggc tttaccttgc
    10381 catggctgga tgtcccggtc gtaaagatta ccaactgtct gttggctccc tctgccaatg
    10441 aacttggcga accacaggaa gaacaaagct gcatccggtg tagcgcctgt gctgacgcct
    10501 gccctgtgga tcttttgccg caacagttgt actggttcag caaaggtcag caacacgata
    10561 aagctaccac gcataacatt gccgactgca ttgaatgtgg ggcttgcgcg tgggtttgcc
    10621 cgagcaatat tcccttggtg caatatttcc gtcaggaaaa agctgaaatt gcggctattc
    10681 gtcaggaaga aaagcgcgcc gcagaagcca aagcgcgttt cgaagcgcgc caggctcgtc
    10741 tggagcgcga aaaagcgact cgccttgaac gacataagag cgcagccgtt caacctgcag
    10801 ccaaagataa agatgcgatt gctgccgctc tggcgcgggt gaaagagaaa caggcccagg
    10861 ctacacagcc tattgtgatt aaagcgggcg aacgcccgga taacagtgca attattgcag
    10921 aacgggaagc ccgtaaagcg caagccagag cgaaacaggc agaactggag caaactaacg
    10981 acgcagcaac cgttgctgaa ccacgtaaaa ctgccgttga agcagctatc gcccgctcca
    11041 aagcgcgcaa gctggaacag caacaggcta atgcggaacc agaagaacag gtcgatccgc
    11101 gcaaagc
//
LOCUS       DAJTYH010000120        11101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTYH010000120 DAJTYH010000000
VERSION     DAJTYH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            317..1957
                     /gene="trg"
                     /locus_tag="OO609_003334"
     CDS             317..1957
                     /gene="trg"
                     /locus_tag="OO609_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6634636.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1995..2918)
                     /locus_tag="OO609_003335"
     CDS             complement(1995..2918)
                     /locus_tag="OO609_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6634637.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3135..4478
                     /locus_tag="OO609_003336"
     CDS             3135..4478
                     /locus_tag="OO609_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6634638.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4703..6358
                     /gene="opgD"
                     /locus_tag="OO609_003337"
                     /gene_synonym="mdoD"
     CDS             4703..6358
                     /gene="opgD"
                     /locus_tag="OO609_003337"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6634639.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6498..6722
                     /locus_tag="OO609_003338"
     CDS             6498..6722
                     /locus_tag="OO609_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6634640.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6785..7321
                     /gene="rimL"
                     /locus_tag="OO609_003339"
     CDS             6785..7321
                     /gene="rimL"
                     /locus_tag="OO609_003339"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6634641.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7316..8296)
                     /locus_tag="OO609_003340"
     CDS             complement(7316..8296)
                     /locus_tag="OO609_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634642.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8420..9412
                     /gene="tehA"
                     /locus_tag="OO609_003341"
     CDS             8420..9412
                     /gene="tehA"
                     /locus_tag="OO609_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6634643.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9409..10002
                     /gene="tehB"
                     /locus_tag="OO609_003342"
     CDS             9409..10002
                     /gene="tehB"
                     /locus_tag="OO609_003342"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6634644.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10305..10961
                     /locus_tag="OO609_003343"
     CDS             10305..10961
                     /locus_tag="OO609_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6634645.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10979..>11101)
                     /locus_tag="OO609_003344"
     CDS             complement(10979..>11101)
                     /locus_tag="OO609_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6634646.1"
                     /translation="ISVIRQYIEQQQTPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         2861 a         2594 c         2798 g         2848 t
ORIGIN      
        1 atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc
       61 acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca cgaaattgcg
      121 tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct
      181 tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg
      241 tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg
      301 acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat
      361 caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag
      421 ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc
      481 ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt cagcccggat
      541 caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa tgaagcgaaa
      601 tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa
      661 tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc aacgctttca
      721 ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc
      781 gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt
      841 gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac tggcccatca
      901 gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac
      961 gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgccgcaca
     1021 acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg cgggtcgtaa
     1081 tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg ggatgacagt
     1141 agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa tttcagctgg
     1201 caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac aaactgccgc
     1261 cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag
     1321 caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt
     1381 agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga tcaccgccgt
     1441 catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc
     1501 gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac ggacactcgc
     1561 aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt
     1621 aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca ctattgttga
     1681 tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag
     1741 tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga cgcaacagaa
     1801 tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac aggctgcacg
     1861 attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg
     1921 cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca
     1981 gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat caactggcaa
     2041 ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt
     2101 cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac agcgccagaa
     2161 gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga agtagacagc
     2221 gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc atctgaatgc
     2281 tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc
     2341 gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa cgattcaaga
     2401 aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg accaatgccg
     2461 atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat tgtcgcaact
     2521 tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg taatatcccc
     2581 agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc ttctttacga
     2641 tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat
     2701 tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgccc
     2761 gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa attaagggtt
     2821 tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc
     2881 aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg
     2941 gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat attattaaca
     3001 attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg cgagatcaca
     3061 gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat tactgaccac
     3121 tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa cagttttcgc
     3181 aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg ctggaactgg
     3241 tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa aaaatggtaa
     3301 atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg
     3361 cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac ccggtgagct
     3421 attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg cccattgatg
     3481 atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc cttgagctta
     3541 tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc gatatcttca
     3601 ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt aacgaaacac
     3661 aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag ttagcaacgg
     3721 tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct gatgtggtct
     3781 gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt gaccgggtgc
     3841 agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg ccgccgcgcc
     3901 gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag acggtgttgt
     3961 ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag cagcgtggcg
     4021 tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac gctggaaccg
     4081 ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact tttcctgaca
     4141 gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg acgccttcgg
     4201 gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta attgaacgtg
     4261 gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc gcggcgggga
     4321 tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat gccagtcgcg
     4381 aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt taccaggaag
     4441 cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc cgcctttgca
     4501 aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa aaaaatgccg
     4561 atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt ctagagtgag
     4621 cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc attcacaaca
     4681 ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt caatggctat
     4741 ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag cattcgcggc
     4801 agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc tacagtcaat
     4861 ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct
     4921 ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa aatcgctctg
     4981 gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa tgggattccg
     5041 tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg aaattcactt
     5101 tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat tagaagggca
     5161 aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga
     5221 tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat atcaatacgg
     5281 tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag agttccccga
     5341 ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta ccgtttatgc
     5401 gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt gtgagaaaag
     5461 tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta aacagctggg
     5521 catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga tgtgcgatac
     5581 aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca acggcgagtg
     5641 gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca ccgacaacaa
     5701 cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc aggacattat
     5761 gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt ggggtaaggg
     5821 caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca ttgtctgctt
     5881 ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt atcgtctgta
     5941 ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga cgcgtaccgg
     6001 catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg
     6061 tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa aaggcattga
     6121 gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct atattgaacc
     6181 catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca ctgatccggt
     6241 cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat ggctgtatca
     6301 gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga tgagttaatc
     6361 gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat cccgctcgca
     6421 tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac cgtgtcagcc
     6481 actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt ctgaaaaacg
     6541 aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat gaaattgcca
     6601 gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa
     6661 agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt
     6721 aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat tgactggaga
     6781 taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg ttgcggaaag
     6841 tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac agcagtcgct
     6901 aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc agggtaatgt
     6961 gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag atgaacttat
     7021 cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa tcggctactg
     7081 gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga cattgattca
     7141 tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg tggacaatcc
     7201 gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc
     7261 tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta tcgattcata
     7321 ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact tttggaccac
     7381 ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt aactccacat
     7441 ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca acgtgctcgt
     7501 cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca tgatgtttta
     7561 acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg
     7621 gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact ttagcgcaat
     7681 cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca aaaacttcgg
     7741 cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt gcctggtgga
     7801 caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt tgatggtcgg
     7861 gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg tgaccaaata
     7921 ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt atatatgcac
     7981 cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc tctccccaca
     8041 gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg aacgcaatag
     8101 cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg cgatcgatcc
     8161 agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata atctgccgta
     8221 ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt tcttcactta
     8281 ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta gcaaattctg
     8341 cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac tttataaatt
     8401 aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg caggctactt
     8461 tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca gccaggtttg
     8521 gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca tctggggatt
     8581 attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc tggcggaagt
     8641 tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc
     8701 gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt ttggtgtcgt
     8761 ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat ctcaccctga
     8821 agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat
     8881 ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt
     8941 ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg gagaattacc
     9001 cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg cctgtagtgc
     9061 ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt tcggttatgg
     9121 actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa
     9181 tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct
     9241 ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt ttatctttac
     9301 caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt
     9361 gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt
     9421 gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga agtactggaa
     9481 gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac
     9541 agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt
     9601 atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca cacccgggtc
     9661 gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc gactgtggtg
     9721 ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca acgttgcact
     9781 aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc
     9841 gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga aggctgggag
     9901 agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt
     9961 attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa gcacaaaacg
    10021 cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag catatttttt
    10081 atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt aacttactga
    10141 aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt cgtctgaaaa
    10201 ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag cggcgctttt
    10261 tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt accacatcat
    10321 ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca
    10381 cccagccaga taaatataac aattactctg atttaaaaga aacaacctca gcaacaggta
    10441 aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac agcatcgtct
    10501 ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg cagaaagttc
    10561 tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg cagcgtaaac
    10621 cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt accggtgtag
    10681 ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta gtggttgcgg
    10741 ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt gaaggtgagc
    10801 tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc gaaggtaaag
    10861 acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt attgatgaca
    10921 tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg cgccatcgtt
    10981 agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg ggatataagg
    11041 cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg atgacggata
    11101 t
//
LOCUS       DAJTYH010000121        10858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTYH010000121 DAJTYH010000000
VERSION     DAJTYH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            217..1047
                     /locus_tag="OO609_003345"
     CDS             217..1047
                     /locus_tag="OO609_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6634647.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1188..1961)
                     /gene="uxuR"
                     /locus_tag="OO609_003346"
     CDS             complement(1188..1961)
                     /gene="uxuR"
                     /locus_tag="OO609_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6634648.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2176..3636)
                     /locus_tag="OO609_003347"
     CDS             complement(2176..3636)
                     /locus_tag="OO609_003347"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6634649.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3717..4901)
                     /gene="uxuA"
                     /locus_tag="OO609_003348"
     CDS             complement(3717..4901)
                     /gene="uxuA"
                     /locus_tag="OO609_003348"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6634650.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5241..6584
                     /gene="gntP"
                     /locus_tag="OO609_003349"
     CDS             5241..6584
                     /gene="gntP"
                     /locus_tag="OO609_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6634651.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6835..7737)
                     /gene="fimH"
                     /locus_tag="OO609_003350"
     CDS             complement(6835..7737)
                     /gene="fimH"
                     /locus_tag="OO609_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6634652.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7757..8260)
                     /gene="fimG"
                     /locus_tag="OO609_003351"
     CDS             complement(7757..8260)
                     /gene="fimG"
                     /locus_tag="OO609_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6634653.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8273..8803)
                     /gene="fimF"
                     /locus_tag="OO609_003352"
     CDS             complement(8273..8803)
                     /gene="fimF"
                     /locus_tag="OO609_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6634654.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8813..>10858)
                     /gene="fimD"
                     /locus_tag="OO609_003353"
     CDS             complement(8813..>10858)
                     /gene="fimD"
                     /locus_tag="OO609_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6634655.1"
                     /translation="NRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKN
                     KWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAP
                     VIHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQI
                     FTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQ
                     LADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTN
                     IQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQL
                     TVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKG
                     RDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNL
                     SYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHA
                     NGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTN
                     TLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQ
                     SSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2611 a         2795 c         2661 g         2791 t
ORIGIN      
        1 aattagtaat aagatatgct tttaaattgc atgcaaataa ttttaaatga gcgttttttg
       61 atactttaga gtgtttccta ccctttaata tcactgtgta gagttgatat cactacaaca
      121 gaaacatgca tgtcatttgt ttacttctct ttttactaca gaaaaattta tctatttttc
      181 taaacatcag ttaaatactc ataaaaggta actattatgt ctatgccatt aagcaatgcc
      241 gctaataacc aattcaatac aaataatcac ttccttcatc atccaaaagt cgacagtgag
      301 ttaacacgtc gatatgaata tgcacgcctg gatacggaaa atatctatct tctgcctctt
      361 gctcgtggca ataaccataa ttacgatgga aaaagcgttg tcgaaattag aaaacttaat
      421 attggcaaac agtcctggcc atttaattat gtgacagata catgcagaga attcaatggt
      481 attacaacaa caggacgtat gctctatcga aacttgaaaa tcacctcagc tttagacgaa
      541 atatatggtg gtatatgtaa aaaggcgcat gctgctacag agttagcaga aggtttacgg
      601 ctaaatttat ttatgaagag ccctttcgat cctgttgagg actatacagt tcatgaaata
      661 acattaggcc ctggttgcaa tgttcctggt tatgccggaa ccacgatagg atatatttca
      721 acccttccag cttctcaggc aaaaagatgg accaatgagc agccaagaat tgatatctat
      781 attgatcaga taataactgt ctcaggggta gcaaattctt ccggattcgc ccttgccgca
      841 ttacttaatg ctaatattga gatgggaaat gacccaatca ttggtataga agcctatcct
      901 ggtaccgcgg agatccattc aaaaatggga tataacgtta tccccggtga tgaagatgcg
      961 ccactcaaac gcatgactct gcaaccgtca tctcttccag aactgtttga attaaaaaat
     1021 ggtgaatgga attatattgg taaatagcca ctgagaagaa tgagtactaa tattcaggtt
     1081 atacagatat ttaaacaaaa ccctgcaatt tacaaagtaa tactcattgc gacttcttaa
     1141 tttaagaaag cgcaaggaac gtttaccctt gcgcttatta taataagtca ggcgtcgact
     1201 ttatccagcg gccatgaatc aaagagatag ccgtcaaaat aaatatcgtc aacgttcgag
     1261 aattccagca gacgttgctt aacgttttcc agatgctgcc acattgccag cttcgccgct
     1321 cgggcatctt ttttgattaa cgcggcgagg atctgtttgt gatcgcccaa ccactctttg
     1381 cgatacaggc tgtcatccag atggctgtgc aactggatcc acattggatt gttttcccgc
     1441 cactgccagg actgacggaa taactccacc agcatgctgt tatgcgttgc ttcagcaatg
     1501 gcgagatgga actgcatgtc accgctttcg ctgctacccg gcgcgctgga agccagttca
     1561 cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat cttcgcgggt agcctgcagt
     1621 gcggcaaact cggcgatgtt actttccagt aactgacgcg cctgtaacag ctcaaaagga
     1681 cccgcatcat tgcagacgtt ggcatccgga ctgtctgtgt tgtggctgcc tgagctatca
     1741 agcacataga taccggcacc ccggcgcact tccaccagcc ctttgatctc cagcatgatc
     1801 agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt ctgcaatttc acgctccggc
     1861 ggcagccgtt cgccaggatt gtacggcgtc tttatgatca gatcgtggat catcgccccg
     1921 acttcctggt aaggtctttg cgcagaggtg acagatttca tggtgttgtg tccgttaggt
     1981 gcggttaatc aaatttttgc cggatgcggc gtgaacgcct tatccggcct acaacgggca
     2041 attttacggc ccccgttaat ctcaaactat accattagca ctggaagttg gcacgtacag
     2101 gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt tgattgacca gtaagtctgt
     2161 agggtaatca gttagttaca gcgcagccac acactcgcgc gcaccgcgtt cgcacagctg
     2221 ctgatatgcc gacgtcaccg cgccaacaaa gtcggcattc tgcggcagat catcggcaaa
     2281 aataccgctc aggcccagca gcgctttcac gcggtctgcg ccctgatact gcgcgttaat
     2341 cttctggaac tcggccagca tcgggtcgac cacgtcaatg gcattaccct gctcatccac
     2401 gccctgggtg taacccatcc agccagccac gcccagcgcc aggtgacgcc agctgccgcc
     2461 gttttgcagg tgcagacgca ccgggtccag tagacgctgc ggtaacttct ggctgccgtc
     2521 catcgcaatc tgccaggtac ggtgacgcag agacgggttg ctgaaacgct cgatcagcag
     2581 cgtcgcatag gcgttcaggt ccgtaccttc cggcatcgac agcgttggcg cttgttcctg
     2641 catcatcagg gcaaaggccg ctttgcgata atccgggtta gtcatggtgt cggcaatggt
     2701 ttcatagccg ccgaggtaac cgaggtacgc caggaaagag tggctgccgt tcagcatacg
     2761 cagcttcatc atttcgaacg gcacaacgtc tgcaacgaac tgtgtgccca ctttatccca
     2821 gtccgggcga ccattaacga agttatcttc aatcacccac tgacggaacg gttcgcaggc
     2881 aatggcgcac gggtcgtaaa cacccagctg gtcagcaatt tcctgtaagg tttctggcgt
     2941 cgccgccgga acgatgcggt caaccatggt gcacgggaag gtgacgttct cttcaatcca
     3001 tgccgctagc tgcgggtcac gcgcctgagc cagccccagt accgcgacct tcgccacatg
     3061 gccgttttca cgcacgttat cgcaggacat caccgtaaat gctttcagcc ctttttcacg
     3121 acgcagacgc aaggcttcga cgatgtaacc aatcgcggac ttcggcgcag tcgggttttc
     3181 cagatcgtgc ttgatcagcg ggttattgag atccagttga ccgcttgccg catcagcgca
     3241 gtagcctttt tccgtgaccg ttagagagac aatcgccgtt tgcggacgcg ccatcgcgtt
     3301 gagaatacct tcgcagccgt cgatttccgg atgcagcgct tctttcatcg aaccgataat
     3361 tttcagctcg gtgctttctg cgcctttttc cgctacggtg tacagcagtt gctgtttttt
     3421 caggttttcg atcagcatgc ggtcgttgcc tggcatcagg ttaacttcgc agatgcccca
     3481 gtcgctgtcg gtgctttcca gcagatgatg ggtatacagc gcctggtgcg cgcggtgaaa
     3541 cgccccgcaa ccgagatgca caatgcgtga ttccagacga gaatgatccc atgacgggcg
     3601 ggcaaccggc agattgctgt caacaatagt agtcatcgta aactccagaa tccgtcttca
     3661 gagagtgacg gcaaggaaga aggaaccggc acggcgcgca gccatgccgg tgggtattaa
     3721 cggataaaga aagcgcgctg gatcgccagt tcgacaccgc gaacttcggc caggcctttc
     3781 agacgaccaa ttgcggagta accagggttg gttttcttct tcaggtcgtc cagcatctga
     3841 tgaccgtggt ccggacgcat cgggatcagg tcttctttgc cttccgcttt acgacggtgt
     3901 tcttcttcaa caatcgcttt caccactttg tacatatcaa cgtcaccgtt caggtgcgcc
     3961 gcttcgtgga aggttttcgg gttatcttca cgcatggtgg agcgcagatg ggtgaagtaa
     4021 atacgcggac cgaactgctt gatcatatca accagatcgt tgtcagcacg cacgccgtag
     4081 gaaccggtgc acatggtgaa accgttcgcc atgctgttta cggtatcaac catccactgc
     4141 atatcttcaa tggtggaaac aatgcgcggc aagccgagga tcgggcgcgg cggatcgtcc
     4201 gggtgaaccg ccatacgcac gccaacttct tcagcaactg gaataatcgc tttcaggaag
     4261 acggcaaagt tttcgcgcag tttggctttg tcgatatctt tgtacagctc caggtgctta
     4321 cggaactggt cgagggtata cccttcttcc gcgcccggca gaccggcaat gatgttacgc
     4381 gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc gttcagctgc ctgagcaatt
     4441 tcttcttcgg tgtaatccgc ttccgcgcct ggacgtttca ggatatgcat ttcgaatgca
     4501 gcgaattcga tctggtcgaa gcgcagagct ttggagccgt ctggcagcac gtattcgagg
     4561 tcagtacggg tccagtcgag caccggcatg aagttgtagc acacggtgcg aatgccacac
     4621 tgcgccaggt tgcgcagagt ctgctgatag ttagcgatcc actgctcata gttgccagtg
     4681 tgggttttga tatcttcgtg aattggtacg ctttctacga cagaccacac caggcctgcg
     4741 tcttcaacga tcgccttgcg tttgaggatc tcttctacgg accatacttc gccgttcggg
     4801 atatggtgca gcgcggtaac cacgccagtt gcgcccgcct gacggacatc agctaaagaa
     4861 accggatcgt ttgggccgta ccagcgccag gtctgttcca tgatttttcc tctttcacat
     4921 agtgggttcc acagcaaaga gacgcggaac cttggagttg tttaggtatc cggatgaaca
     4981 accagaccgc agccgtggcg ttgcatcgga tgagatggcg tataagttct accttttggt
     5041 aagacatgtc aaccagaatt ctgaaattgg ttaaccgcgt cgcaagaatt tcactctcat
     5101 ggttgttgct gcaaattgca gcaaagggta atcgatggtt attgatcaga ggatgtgaca
     5161 ttcatcgcaa caatggttga ccaatttaca taacatgtcg cccaaaataa caacggttca
     5221 accaagtaag gtaaaacgtt atgcatgtgc ttaacattct ctgggtggta ttcggcattg
     5281 gcctgatgct gatactgaat ttgaagttca aaatcaattc aatggtggct ttgttggtgg
     5341 cggcgctgtc cgtcgggatg ctggcaggca tggatttgat gtcgctgctg cacaccatga
     5401 aagcgggctt cggcaacacg ctgggagaac tggctatcat cgtggtgttc ggtgcggtca
     5461 tcggtaaatt gatggtcgac tccggcgcgg ctcaccagat agcacatacg ctgctggcgc
     5521 gtctcggtct gcgctatgta cagctgtcgg tgattatcat cggccttatt ttcggtctgg
     5581 cgatgttcta tgaagtggcc tttatcatgt tagcgccgct ggttattgtt attgccgccg
     5641 aagctaaaat tccgttcctg aaactggcga tcccggcagt agcagctgcc actaccgcac
     5701 attcactgtt cccaccgcag ccgggtccgg tggcgctggt gaatgcttat ggcgcggata
     5761 tggggatggt ttatatctat ggcgtactgg tgacgatccc aagtgtaatc tgcgcaggtc
     5821 tgatcctgcc gaagttcctc ggcaatcttg agcgcccaac gccatcattc ctgaaagcag
     5881 atcaaccggt agatatgaat aatctgccct ctttcggcgt ttcgattctg gtgccgctga
     5941 tcccggcgat cattatgatc tctaccacca tcgccaatat ctggctggta aaagataccc
     6001 ctgcctggga agtggttaac tttattggtt cctcgccgat tgcgatgttt attgcgatgg
     6061 tggttgcatt cgtactcttt ggcaccgcgc gtggtcatga catgcagtgg gtgatgaacg
     6121 cttttgaaag cgcggtgaag agtattgcaa tggtgattct gatcatcggt gcgggtggcg
     6181 tgctgaagca gaccatcatc gacaccggca ttggcgacac catcggcatg ttgatgtccc
     6241 acggcaatat ctcgccctac attatggcat ggctgatcac tgtgctaatt cgtctggcga
     6301 cgggtcaggg tgtcgtttcg gcgatgaccg ccgccgggat tatcagtgct gcaatccttg
     6361 atccagcaac cggtcagctg gttggcgtga atccggcgct gctggtactg gcgacggctg
     6421 cgggttccaa caccctcacc cacattaatg atgcatcttt ctggttgttc aaaggttact
     6481 ttgacctgtc agtaaaagac acgttgaaaa cctggggtct gctggagctg gtcaactccg
     6541 tggttgggct gattattgtg ctgattatta gcatggtagc gtaaaagaac accagagcct
     6601 gccaatggca ggctcagact gatgactaaa gtctaaaaat gtgcccggac agtcccctcg
     6661 cccctccggg gagagggtta gggtgagggg aacagaccag taaagtgcgc tttgtttatg
     6721 ccagatgcga cgctgacgcg tcttatctgg cctacaaagg gctaacgtgc aggttttgta
     6781 ggtctgatag cgtagcgcct caggtaccag cattagcaat gcgctgtaat ttttttattg
     6841 ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct gccctccggt
     6901 acgtgcgtaa tttgccgtta atcccagact taccgccgaa gttcctactg ctcctaacga
     6961 taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg
     7021 cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg cggttgtgcc
     7081 ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag gaattggcac
     7141 tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat cacaaccgcc
     7201 agtgggcacc accacatcat tattggcgta aatattccac acaaactgaa aatcatcgct
     7261 gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgaaccag ctttaatagc
     7321 caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg gcttatccgt
     7381 tctcgaatta taaacaaccc gcggcgtttc gctggtggtc ggaaatggat agctactgcc
     7441 actatatttt acggtcccgg aaaaattaga taacacgccg ccgtaagccg agcctcgttg
     7501 cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga tttgcgtcga
     7561 aagatctacg accaggtttt gccccacatt cacgacaggc gcaaggttta cataaacatt
     7621 agcgctgcca ccgccaatag ggatagcggt accattggcg gttttacagg cgaatgacca
     7681 ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaatagctc gtttcattac
     7741 aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc tgaatggttc
     7801 cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag tgcgctgatt
     7861 gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg ttgccactgt
     7921 catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa tatccggtgc
     7981 tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga caattagtca
     8041 actcaagcgc aacatcatgc caggccgatg ccgccccggc agacataaga ctgaaagaat
     8101 aaagatcgcc gagatctacc gtggcattgg tggtggaaac cgtacacggt ttggcgacga
     8161 ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt gcgagcgcca
     8221 acattgccgc caatacatac ccacgtttgc accatttcat gagcatctcc agttactgat
     8281 attcaagagt gaaggtagcc gtagcattga tatgccccgc agtgacaggc acctgtgtcg
     8341 ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc cggcgtcagg gtcgtccacg
     8401 aaatcgcaga cgatggagca ttaaggggta tttgattttg ctgctcattc agaagctgta
     8461 ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc aagcaggttg gcattgtggc
     8521 tatctgcaac gccggtaaac ccaactttta cggcagaaac ggcattacca cagggtgaca
     8581 gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat gttgttaaat tgcttcgccg
     8641 cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta cagccgttat
     8701 ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga cttaccgcca
     8761 gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc ccttaacgac
     8821 attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt tgataattgg
     8881 cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc gctaaaggca
     8941 ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg ctactctctg
     9001 atgtcaccat cgccccaaac ggcagcggct tattattgtg agttagcgtc atgagcagtt
     9061 ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg ggaacaacgt
     9121 tagcgaccgc gttatctaaa tcgacgttat cagccaaggt attggtatcc agcgccactc
     9181 tattttcccg atattcagtg gcataaggca gcacggcata accacgccag tcggtacgca
     9241 cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca agcaccaccg
     9301 tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccaatacc ccaccgctga
     9361 ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg gcattgccgt
     9421 aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctattacca tcgcctcccc
     9481 cggcatagcc ggtttgcacg ctataactga ggttgttgtc ttccagcaac gtaccgtata
     9541 caccagccag attggtcatc cgaccgttga gatcgtgtga catgctgtag ctggcactgg
     9601 catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga atattgacgt
     9661 taagcgctaa catctgatcc cgtccttttt gccaggcgtt tttcgtcagg ctatagctga
     9721 gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctggaattgc tcatcgacat
     9781 tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat gtgcgcccga
     9841 gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg ttgtaatagt
     9901 cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg tagccattca
     9961 ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa cggtaaccca
    10021 ctaactgaat attcgtgcct gattcattga gcgatttgtt atagagaaaa cgcaccgatt
    10081 gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata tccacagaca
    10141 gagcgcccag tgcctccatg tttttcccga taccgaaatt aaaagcacga taacgatccg
    10201 ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt aatgtactct
    10261 ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg gtattctcct gccgtaatgg
    10321 aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt acggtaaaaa
    10381 tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta ttacctgcgg
    10441 catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa atgccatatc
    10501 cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc accggggcaa
    10561 atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg ccgcgaaagt
    10621 taataccatc gaaaatatcg ccctgggtat aaccatcacc caacgtcagc cgggaacgta
    10681 aaggaattat gtctcgctca atccaggtaa tgatatgctg ccatttattt ttgctacctg
    10741 atgaactgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc cacgcaccaa
    10801 tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca atccgatt
//
LOCUS       DAJTYH010000122        10858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTYH010000122 DAJTYH010000000
VERSION     DAJTYH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO609_003354"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="OO609_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6634656.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="OO609_003355"
     CDS             complement(1433..1759)
                     /locus_tag="OO609_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6634657.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="OO609_003356"
     CDS             1894..2235
                     /locus_tag="OO609_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6634658.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="OO609_003357"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="OO609_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="OO609_003358"
     CDS             complement(2833..3543)
                     /locus_tag="OO609_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6634659.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="OO609_003359"
     CDS             3651..3956
                     /locus_tag="OO609_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6634660.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="OO609_003360"
     CDS             4155..4475
                     /locus_tag="OO609_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR6634661.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="OO609_003361"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="OO609_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6299..7288)
                     /locus_tag="OO609_003362"
     CDS             complement(6299..7288)
                     /locus_tag="OO609_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634662.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYVLQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7373..7864)
                     /locus_tag="OO609_003363"
     CDS             complement(7373..7864)
                     /locus_tag="OO609_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634663.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8185..8685)
                     /locus_tag="OO609_003364"
     CDS             complement(8185..8685)
                     /locus_tag="OO609_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634664.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8675..9190)
                     /locus_tag="OO609_003365"
     CDS             complement(8675..9190)
                     /locus_tag="OO609_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634665.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9621..9989)
                     /locus_tag="OO609_003366"
     CDS             complement(9621..9989)
                     /locus_tag="OO609_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634666.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10401..>10858)
                     /locus_tag="OO609_003367"
     CDS             complement(10401..>10858)
                     /locus_tag="OO609_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6634667.1"
                     /translation="EVCHLTKRDIVTLDNGLVCIDINEDGDGKSVKNKHSVRLVPLTD
                     GAYGFDLTSFLSWVDMQPDEGPLFGMTPSAYSSWFNSRVLTEALGDSQDVSLHSLRHW
                     LATRMKERGVNLVDAQGILGHSSQSITYDLYGKGHAVGRLADVLKTALL"
BASE COUNT         2790 a         2757 c         2677 g         2634 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga
     6001 caactagtac aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt
     6061 gacaagccac cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc
     6121 ttacctccct cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga
     6181 ggagctacag gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca
     6241 cctgtcaggc ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt
     6301 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc
     6361 cagtgcccat cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt
     6421 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     6481 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct
     6541 tgtcttcacc gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac
     6601 cggagaagcc ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga
     6661 taataccgta gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt
     6721 ccatgatagc gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct
     6781 tcagcagctt agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat
     6841 tctcacagat gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact
     6901 tggcgtccca gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca
     6961 cgatttcttg tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa
     7021 cgaccacaga gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg
     7081 tcggcaggac ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca
     7141 gcatagcctt aaccgtctta gctaactgga ggacatagac ctgcgggtca gcgttagtcg
     7201 gcagaccttc attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa
     7261 actggaaagc gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact
     7321 gtgagtcctc aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc
     7381 tgtccatcca gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag
     7441 cggaatgggt agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt
     7501 cgcggcgctg tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct
     7561 cagcctgaag tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc
     7621 ggcgaccata ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt
     7681 cagccagagc ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt
     7741 agcccggaat gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca
     7801 cagtgttttc ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca
     7861 tcataggtaa ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct
     7921 acgggtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga
     7981 ctgcttagca aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat
     8041 cagctcgtct cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga
     8101 cagcagatga cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg
     8161 agggtcaatc atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg
     8221 atagtctgaa tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg
     8281 acgctggatg aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg
     8341 cacggtggcg ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag
     8401 gtccaaccac cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa
     8461 gtgacccatg ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc
     8521 cttggtcctc atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg
     8581 aatgcctacc cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg
     8641 accgaagata tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg
     8701 ccagagcttc ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt
     8761 cggcatcagg tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct
     8821 gaaggcggcg ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt
     8881 cggcatcaga gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag
     8941 gattgttgaa atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct
     9001 ggaggtatga ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt
     9061 ccgcctgggc ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga
     9121 cactggactc tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt
     9181 cggtattcat acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc
     9241 accattcaga cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta
     9301 aatgttgacg gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt
     9361 caggacgagc tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga
     9421 gtagacgagc tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg
     9481 aggtctgtca gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg
     9541 accagatgct cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag
     9601 tgatagttta gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta
     9661 ctggcgctga cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca
     9721 cccaaggcaa cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg
     9781 tgcgttagta gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt
     9841 gattcgttca ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc
     9901 tgaccatcga atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg
     9961 ttgtcgtgag ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt
    10021 cccctatagt gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg
    10081 actcacaggt tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat
    10141 ataaagagag accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga
    10201 atttggaggg actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc
    10261 ggacagccga aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc
    10321 tccctaactt cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc
    10381 tccaccttgg gtctccctac ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc
    10441 acagcgtgtc ctttcccgta caggtcatac gtaattgact gactgctgtg acctaagatg
    10501 ccttgagcgt ccaccagatt gactccacgc tctttcattc gggtcgccag ccaatgtctc
    10561 agactgtgga gagagacatc ctgagagtct cctagggcct cagtcaggac ccttgagtta
    10621 aaccagctag agtaggcgct cggagtcatc ccgaagagag gaccctcatc aggctgcatg
    10681 tctacccacg acaggaagga cgtcaggtca aacccataag cgccatcggt taacggcaca
    10741 agtcggactg aatgtttgtt cttcacagac ttcccgtccc catcttcatt gatgtcgata
    10801 cagaccagtc cattgtccaa ggttacaatg tcacgtttag tcaggtggca gacctcag
//
LOCUS       DAJTYH010000123        10814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTYH010000123 DAJTYH010000000
VERSION     DAJTYH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..285
                     /locus_tag="OO609_003368"
     CDS             <1..285
                     /locus_tag="OO609_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6634668.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWM
                     ENTIFPAMKDIPVLSGLIDTMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OO609_003369"
     CDS             296..1036
                     /locus_tag="OO609_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6634669.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OO609_003370"
     CDS             1095..1481
                     /locus_tag="OO609_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6634670.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OO609_003371"
     CDS             1490..1819
                     /locus_tag="OO609_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6634671.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..4832
                     /locus_tag="OO609_003372"
     CDS             1791..4832
                     /locus_tag="OO609_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6634672.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4832..5161
                     /locus_tag="OO609_003373"
     CDS             4832..5161
                     /locus_tag="OO609_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6634673.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5161..5859
                     /locus_tag="OO609_003374"
     CDS             5161..5859
                     /locus_tag="OO609_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6634674.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5864..6607
                     /locus_tag="OO609_003375"
     CDS             5864..6607
                     /locus_tag="OO609_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6634675.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6544..7146
                     /locus_tag="OO609_003376"
     CDS             6544..7146
                     /locus_tag="OO609_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6634676.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7207..10686
                     /locus_tag="OO609_003377"
     CDS             7207..10686
                     /locus_tag="OO609_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6634677.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10754..>10814
                     /locus_tag="OO609_003378"
     CDS             10754..>10814
                     /locus_tag="OO609_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCR6634678.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2545 a         2560 c         3466 g         2243 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgcgcag tacaccggaa aaatgacgga tgaggacgac
       61 tggcaggctg ttctgcatat tgccgttttc gtccgtgcac aggcgccgga ttctgagctg
      121 gatacgtgga tggagaacac catatttccg gccatgaagg atattccggt gctttccgga
      181 ctcatcgaca ccatgatccc actcggtttt aactatcaac gtgataatga gatggccacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg
     4621 atggtaagct gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc
     4681 agtacaacat tgagatcaat aacgacggca cgaacgggca gataggtccg gctgccctga
     4741 aggtggttta tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg
     4801 atggtggccg gttaagtgga gcataccggt aatggagacg tttcactgga aagtgcgccc
     4861 ggatatgaat gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga
     4921 acagcgtcgt gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt
     4981 tcgtaaaggt gaacacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc
     5041 atttcagtgg acgccacctt atgactggaa actgatcagg gtggtttgtc gtaaatggtc
     5101 ggcaagcgtg ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg
     5161 atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcagcccgg
     5221 gtggagctct gggaaatcga tctgacagag gtcggtggtg agcgttattt tttctgtaat
     5281 gagcagaacg aaaaaggtga gccggtcacc tggcaggggc ggcagtatca ggcatacccc
     5341 attcaggggt cggggtttga actgaacggc aggggctgtg ctgcccgtcc gacactgacg
     5401 gtctctaacc tgcacggcat ggtcaccggg atggcggaag atctgcagag tctggtcggc
     5461 ggaacggtgg tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac
     5521 ggaaacagcg acgccgatcc ggaacaggag gtgatcagcc gctggcgcat cgagcagtgc
     5581 agcgaactga gcgcggtcag tgcctctttt gtgctggcca caccaacgga gacggatggc
     5641 gcggttttcc cggggcgtat catgctggcg aatacctgta tgtggactta ccgttctgat
     5701 gagtgtggtt acacgggcag ggcagtggct gacgagttcg acaaaccaac aacggatatc
     5761 cggaaggaca aatgcagcaa gtgtatgcgc gggtgtgagt tgcgcaacaa taccggtaat
     5821 ttcggcggtt tcctttccat caataaactt tctcagtaaa tccatgacac agacagaatc
     5881 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgtggct tcgtggtgag
     5941 aacgccggag ggagacaggt atcttcccag cgagaatatc tccggtgagc cggaggaacg
     6001 gttccggatg gctccggagg actggctgcg ggcacaaatg cagggtgaga ttgtggcact
     6061 ggtccacagt catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt
     6121 gcagagtgat ttgccgtggt ggctggtctg ccgggggacg attcacaagt tccgctgtgt
     6181 gccgcatctc accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     6241 ggatgcttat catctggcgg ggattgagat gccggatttt catcgcgggg atgactggtg
     6301 gcgtcacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc
     6361 gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa
     6421 tcatgccgcc atttactgcg gcgatggtga gctgctgcac catattcctg aacaactgag
     6481 taaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
     6541 ggaatggcac gcatctgcct ttacggggat ttgcaacgat ttggccgccg catcgacctt
     6601 cgtgtgaaaa cgggggctga agccatccgc gcactggcca cacagctccc ggcgtttcgt
     6661 cagaaactga gtgacggctg gtatcaggtg cgcattgccg ggcgtgatgc aggtgaaacc
     6721 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg cagtgatcca catagtaccg
     6781 cgtctggtgg gagctaaaag tggcggtgtg tttcaggcgg tgctgggggc agctgttatg
     6841 gcggttgcta tatggatgcc gggggtagga attatggcga gtaatctgct gttttctctc
     6901 ggtgccagta tgacgcttgg cggtgttgca cagatgctgg caccgaaagc cagaactccc
     6961 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
     7021 gttgcccagg gcaatgttct gcctgttctg tacggtgaaa tgcgcgtggg gtcgcgggtg
     7081 gtatctcagg agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt
     7141 cgctgatgca aaatgtttca tgtgaaaccg cctgcgggcg gttttgtcgt ttatggagcg
     7201 tgaggaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
     7261 aaatccacgc agttgctgag tgtgattgat gccatcagcg aagggccggt tgacggtccg
     7321 gtggatggat taaaaagcgt gctgctgaac ggtacgccgg tcctggatag cgaggggaag
     7381 acaaactttt ccggtgttac ggtggtgttc cgcgccggcg agcaggagca gacaccgccg
     7441 gaggggtttg aatcttccgg ctcagagact gtgctgggta cggaagtgaa atacgacacg
     7501 ccgatcaccc ggaccatcac gtcggcaaac attgatcgtc tgcgcctgac cttcggtgtg
     7561 caggcactgg tggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     7621 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     7681 accacttcgc agtatctggc ctcggtggtg gtgggtaacc tgccgccgcg cccgtttaat
     7741 atccggatgc gcaggatgac accggacagc accacagacc agctgcagaa caaaacgctc
     7801 tggtcgtcgt acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgctggtc
     7861 ggcgtgcagg tggattcaga gcagttcggt aaccagcagg tgagtcgcaa ttatcatctt
     7921 cgcgggcgca ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt
     7981 atctgggacg gaacgcttaa gccagcatac agcaacaaca tggcctggtg tctgtgggac
     8041 atgctcactc atccgcgcta cggcatgggg aaacgtcttg gtgcggcaga tgtggacaaa
     8101 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg atttggcggc
     8161 acggagccgc gcatcacctg taacgcttac ctgaccacac agcgtaaggc gtgggatgtt
     8221 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     8281 ttcgtgcagg accgaccgtc ggataaagtg tggacctata accgcagtaa tgtggtgatg
     8341 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgtc
     8401 gttgaggtga actggattga tccggacaac ggtcatgaga cggcgacaga gcttgtggag
     8461 gacacgcagg ccattgtccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     8521 accagccggg gacaggcgca ccgcgccggg ctgtggctga ttaaaacgga actgctggaa
     8581 acgcagaccg tggacttcag cgtcggcgca gaagggcttc gccatgtacc gggtgatgtc
     8641 attgaaatct gcgatgatga ttatgccggt atcagcaccg gtggtcgcgt actggcggtg
     8701 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccgtc ctccggtacc
     8761 acgctgataa gcctggttga cggaaatggc aatccggtca gcgtggaggt tcagtccgtc
     8821 accgacggcg tgaaggtgaa agtgagccgg gttcctgacg gcgttgcaga atacagcgtg
     8881 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     8941 aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg tgccggaaaa agaggccatc
     9001 gtggataacg gggcacactt tgacggtgac cggcgcggca cggtgaatgg tgtcacgccg
     9061 ccagcggtgc agcatctgac cgccgaagtc accgcagaca gcggggaata tcaggtgctg
     9121 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     9181 gcagcggacg acggcagtga gcggctggtc agcacagcca ggacgacgga aaccacatac
     9241 cgcttcaggc agctggcgct ggggaattac agtctgacag tccgggcggt aaatgcccgg
     9301 gggcagcagg gcgatccggc gtcggtatcg ttccggattg cggcaccggc agcgcctgtc
     9361 actattgaac tgataccggg gtattttcag ataacggtgg tcccgaaact ggctgtatat
     9421 gacccgacgg tgcagtttga gttctggttt tcggaaaagc ggattgcgga tatcaggcag
     9481 gttgaaacca gcgcccgcta tcttggcacg gcgctgtact ggatagccgc cagtatcaat
     9541 atcaggccgg gccatgatta ttatttttac gttcgcagtg tgaacaccgt tggcaaatcg
     9601 gcatttgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct gagtttttat
     9661 aaagggttga tcaataaaac gcatctcggc aaggagttgt ggacgcagat tgataacggt
     9721 cagcttgcgc cggacctgac tgaaatcagg acgtccatta cgaatgtcag caatgaaatc
     9781 acgcaaaccg tcaataaaaa actggaaaat cagagtgctg caatccagca gatacagaaa
     9841 gttcaggttg atacaaataa taacctgaac agcatgtggg ctgtgaagct gcaacagatg
     9901 aaggacggac gcctttatat tgcgggtatc ggtgccggta ttgagaatac gccagcagga
     9961 atgcagagtc aggtgctgct ggcggcagac aggattgcga tgattaatcc tgcgaatggc
    10021 aacacaaagc cgatgtttgt tggtcagggc gatcagatat tcatgaacga cgtgttcctg
    10081 aaacgcctga cggctccgac cattaccagc ggcggtaatc ctccggcatt ttccctgaca
    10141 cctggcggac ggctgacggc gaaaaatgcc gatatcagcg gtaacgtgaa cgcgaactcc
    10201 gggacgctca acaacgtcac gattaataag aactgtcggg ttctgggaaa attgtccgcg
    10261 aaccagattg aaggcgatct cgttaaaaca gtgggcaaac ctttcccacg ggactcccgg
    10321 gcaccggaga ggtggccatc agggaccatt accgtcaggg tttatgacga tcagccgttt
    10381 gatcggcaaa ttgttattcc cgcggtggcg tttcgcggtg ctaaacatga gcggaagaat
    10441 aacaatattt attcgtcatg ccgcctgata gtgaagaaaa acggtgctga aatttataac
    10501 cgaacgaccc tggataatac gctgatatat acgggtgtta ttgatatgcc tgccggtcac
    10561 ggtcacatga cgctggagtt ttctgtatcg gcatggctgg taaatggctg gtatcccaca
    10621 gcaagtatca gcgatttgct ggttgttgtg atgaagaaat ccacagcagg tatcagtatc
    10681 agctgaattt tataacccag atacgggcac cagaaatggt gcctttttta ttgcagaaaa
    10741 gcgagaggta attatgcgta aactttatgc tgccattttg tccgcagcca tctgtctggc
    10801 tgtatccggt gcgc
//
LOCUS       DAJTYH010000124        10631 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTYH010000124 DAJTYH010000000
VERSION     DAJTYH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            145..495
                     /locus_tag="OO609_003379"
     CDS             145..495
                     /locus_tag="OO609_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6634679.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            548..943
                     /locus_tag="OO609_003380"
     CDS             548..943
                     /locus_tag="OO609_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6634680.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            984..1727
                     /gene="cmoA"
                     /locus_tag="OO609_003381"
     CDS             984..1727
                     /gene="cmoA"
                     /locus_tag="OO609_003381"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6634681.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1724..2695
                     /gene="cmoB"
                     /locus_tag="OO609_003382"
     CDS             1724..2695
                     /gene="cmoB"
                     /locus_tag="OO609_003382"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6634682.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2860..5289)
                     /gene="torZ"
                     /locus_tag="OO609_003383"
     CDS             complement(2860..5289)
                     /gene="torZ"
                     /locus_tag="OO609_003383"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6634683.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5314..6414)
                     /locus_tag="OO609_003384"
     CDS             complement(5314..6414)
                     /locus_tag="OO609_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6634684.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6802..7548)
                     /gene="cutC"
                     /locus_tag="OO609_003385"
     CDS             complement(6802..7548)
                     /gene="cutC"
                     /locus_tag="OO609_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6634685.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7562..8128)
                     /locus_tag="OO609_003386"
     CDS             complement(7562..8128)
                     /locus_tag="OO609_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6634686.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8344..10077
                     /gene="argS"
                     /locus_tag="OO609_003387"
     CDS             8344..10077
                     /gene="argS"
                     /locus_tag="OO609_003387"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6634687.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10254..>10631
                     /locus_tag="OO609_003388"
     CDS             10254..>10631
                     /locus_tag="OO609_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6634688.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYS"
BASE COUNT         2585 a         2724 c         2552 g         2770 t
ORIGIN      
        1 tttaatccta tcgtttattg cttgcatagt agaatctaac catccatcac tcatagctaa
       61 ccctcggaaa tcttacattt ctgtctatca taaccactag catcaccttt aggaaccatc
      121 tgaaacggga ggtgtattta gaccttgcta atcttagaca gccgcccggt tcatgcatca
      181 cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat
      241 gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga tatgacgcaa
      301 aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca gaccactacg
      361 ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt acataccctg
      421 tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa
      481 tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg
      541 agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa tgaagttctc
      601 ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg gtggttttag
      661 cgaactgcaa agcgctattt gcattcatgg taacgcggtg gaatatattc ccatcgcgat
      721 tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg
      781 catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg
      841 ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa
      901 tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt
      961 tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg
     1021 ccagactggg cgactggacc tttgatgaac gggtcgctga agtcttcccg gatatgatcc
     1081 agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct
     1141 tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct
     1201 cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg
     1261 cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg ccagtagacg
     1321 tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt
     1381 ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag
     1441 gactgaaccc gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag
     1501 ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac
     1561 tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa
     1621 cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct
     1681 ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa
     1741 cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca
     1801 gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacacggt
     1861 ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga
     1921 aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcggaacct
     1981 gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg
     2041 ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat
     2101 tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca
     2161 cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa
     2221 actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc
     2281 actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct
     2341 ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac
     2401 gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat
     2461 gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg
     2521 ttttgtagat atccgcattg tggatgtgtg cgttaccact actgaagagc agcgacgcac
     2581 tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac
     2641 agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc
     2701 tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc ttgctaattc
     2761 aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt
     2821 gcataacctc agcgcccgtt tccgggcgct attcacttct tacgcctgaa cagcaggctt
     2881 atcaaatgcc gttaacttcg gtgcgttacc cgtatatttt tcgatataca ccagcgcaga
     2941 gttaccggca caggcatttg cccgctgcga gctggggata tccgccgtta acacgttcgc
     3001 actgccgttt ttacacaagc cgttttccag atctggccat gcaccttcat gcacgcatac
     3061 cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt
     3121 ccagacacgc accagatcgc cattcgcaat accaaatcga gcagcatctt cggtgtgaat
     3181 agtgatcggt tcacgatcgg caaccgcata ttttttacgc agttccgcat agttaagctg
     3241 actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt
     3301 acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa
     3361 tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca gcgcattttt
     3421 caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg
     3481 catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg
     3541 cgcacgggcc cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt
     3601 accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat cggcaaacac
     3661 gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg
     3721 ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg
     3781 taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa cgacgatcat
     3841 ctccggtttc tgccaggctt tgatcagacg attagtatcc tggtgatggg taaagttacc
     3901 gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac cgttatgctg
     3961 atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccgtcat
     4021 tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag
     4081 cacgccgcca acacgcgtcg ggttgccacc attggagtaa tgataagaga aaccaaaacc
     4141 gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt
     4201 ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg tgcgattcgc
     4261 cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgt
     4321 ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt caaactgcgg
     4381 atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt
     4441 atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc
     4501 attatcgcca aagaattcga tggtttcgga gcggatagga tcaatggcaa tcactggttt
     4561 gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc
     4621 aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc tgttttccag
     4681 aatcagcggc caggaggcct gctgttcata cacctctaca gaaccgacca catgcggcat
     4741 gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag
     4801 gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc
     4861 gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct catgttgctc
     4921 atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt cgccacgacc
     4981 tttcgccggt tgcagtggat tgtcgagata gctttttctc accatcggat gctgaatacg
     5041 cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta tggttttcgc
     5101 cagcgcgcct gtcgaagaaa caatcttgcc gtccttcact tctacgctca tcgctcccca
     5161 acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga gcggtgctgc
     5221 cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt cacgtcttgt
     5281 taatgccata acttcctccc tgatcaacga ggatcactgt ttctcggtaa tatctttggc
     5341 gttgtactgg aaataccgcg ttaaaatgtc cggttcgttt tcgctcatgc tggttcgtgc
     5401 ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg cggcaataga
     5461 ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc atagcggttt
     5521 acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac gccattgatt
     5581 gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac cttcttcact
     5641 taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt cactgccctg
     5701 catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa cgacggtgag
     5761 atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga aaggataaat
     5821 atgtgcgcca ttagtcactg aagtagcggc ctgactttcc agctcatgcg ccgcgttgtc
     5881 atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga tacaggtttc
     5941 gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg aggcaatatc
     6001 catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt ttaattcttt
     6061 ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac tgtctatttt
     6121 gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa ataaataatc
     6181 catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt tctggttcga
     6241 aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc aggaaaggca
     6301 aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc cgccgccacc
     6361 aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc gcatagattc
     6421 ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa cgaattaacc
     6481 gcttgcataa ctcggcacaa cactgcctga aataatcgat aaagaatatg atttattaca
     6541 atgtaatcat taattgctaa ggaataaccc agttgctatt tagaatatat atttatcagt
     6601 tttagcaatt taaatcccat aatcaatgtg aatataaaaa atatttttag cacaacgcta
     6661 tgcgcactgt cacccgctca acagatcacg gttatcgttc gttttttata ctgctcaggg
     6721 ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc
     6781 gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc
     6841 agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt catctgatga
     6901 catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct
     6961 atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc
     7021 tccagccata atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc
     7081 ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgcaag
     7141 attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc
     7201 cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac
     7261 atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc gcacatcctc
     7321 aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaattat
     7381 cggatgcaca gggatcgtca cccgctggcg cacggatttc agtaccccca acgacggcgt
     7441 taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc
     7501 cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta
     7561 attacgctga ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatag
     7621 ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg
     7681 gtgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca
     7741 gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata tgttcccaac
     7801 cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacattggaa aactgccagt
     7861 gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcattt
     7921 tttctgacaa aagctcgcca cactgttcaa aaccgcgacg ccagcgctca gcggtgacgt
     7981 tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccagac
     8041 ggcgggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca
     8101 gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa
     8161 tttaccctgt tgccctgtgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt
     8221 gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc
     8281 cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt
     8341 ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc
     8401 gcgcctgcgg attgcgaacc gcagattcgt cagtcagcaa aagttcagtt cggcgactat
     8461 caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca
     8521 gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt
     8581 ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg
     8641 ctggcgtccg atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac
     8701 tctgcgccaa acgtggcgaa agagatgcac gtcggtcacc tgcgctctac cattattggt
     8761 gacacagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc
     8821 ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa
     8881 aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat
     8941 tacgacgaag atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc
     9001 ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac
     9061 cagatcactt acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggcgaaagc
     9121 ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta
     9181 gaaagcgaag gggcgaccgt cgtattcctt gatgtgttta aaaacaagga aggcgaaccg
     9241 atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc
     9301 tgtgcgaaat atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc
     9361 cgtcagcatc aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg ctatgtaccg
     9421 gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg
     9481 ttcaaaaccc gcgcgggtgg taccgtgaaa ctggcagacc tgctggatga agccctggag
     9541 cgtgcacgtc gtctggtggc agaaaagaac ccggatatgc cagctgacga gctggaaaaa
     9601 ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc
     9661 acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac
     9721 atgcagtatg cttacacgcg tgtattgtcc gtgttccgta aagcagaaat taacgaagag
     9781 caactggctg cagctccggt aatcatccgt gaagatcgtg aagcgcaact ggcagctcgc
     9841 ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg
     9901 tgtgcttacc tgtacgatct ggccggtctg ttctctggct tctacgagca ctgcccgatc
     9961 ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacagcg
    10021 aagacgctga agctgggtct ggatacgctg ggtattgaga ctgtagagcg tatgtaatcg
    10081 atttttcgtg agagtgaagc ctgatcaggg ttagccgatc aggctttttt attgccatct
    10141 aaatgtattc tgaaaacgga catgccattg ttttctcact gttgagtaag aggccagaag
    10201 cgtaatatcc aggcccagga aaacgataac ggttcaattt aaggaatacc gaagtgttta
    10261 aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa gatacggtta
    10321 tagtaaacga ccatgacact tcagccatca aagattgttg gcaaaaaaat tcagatgatg
    10381 atactgacat taacgtgatc aaatcatgcc tgcgacaaga atacaatctc gtcgatgcgc
    10441 aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca cgcacaggtg
    10501 caaaaaaact tgatactgaa caacttaact tgcttaaaaa atcacagcga gcctggctgg
    10561 attttaggga caaagaatgt gaattaatcc tttcaaatga ggatgttcag gatttaagtg
    10621 acccctattc t
//
LOCUS       DAJTYH010000125        10586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTYH010000125 DAJTYH010000000
VERSION     DAJTYH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..281)
                     /locus_tag="OO609_003389"
     CDS             complement(<1..281)
                     /locus_tag="OO609_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR6634689.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSF"
     gene            complement(443..517)
                     /locus_tag="OO609_003390"
     tRNA            complement(443..517)
                     /locus_tag="OO609_003390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:cct)
     gene            complement(591..1523)
                     /locus_tag="OO609_003391"
                     /pseudo
     CDS             complement(591..1523)
                     /locus_tag="OO609_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1817..2572
                     /gene="mlaA"
                     /locus_tag="OO609_003392"
     CDS             1817..2572
                     /gene="mlaA"
                     /locus_tag="OO609_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6634690.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2754..3812)
                     /locus_tag="OO609_003393"
     CDS             complement(2754..3812)
                     /locus_tag="OO609_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634691.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4178..5518)
                     /gene="fadL"
                     /locus_tag="OO609_003394"
     CDS             complement(4178..5518)
                     /gene="fadL"
                     /locus_tag="OO609_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6634692.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            5890..6174
                     /locus_tag="OO609_003395"
     CDS             5890..6174
                     /locus_tag="OO609_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6634693.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6354..7664
                     /gene="fadI"
                     /locus_tag="OO609_003396"
     CDS             6354..7664
                     /gene="fadI"
                     /locus_tag="OO609_003396"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6634694.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7664..9808
                     /gene="fadJ"
                     /locus_tag="OO609_003397"
     CDS             7664..9808
                     /gene="fadJ"
                     /locus_tag="OO609_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6634695.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10011..10496
                     /gene="sixA"
                     /locus_tag="OO609_003398"
     CDS             10011..10496
                     /gene="sixA"
                     /locus_tag="OO609_003398"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6634696.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2625 a         2449 c         2666 g         2846 t
ORIGIN      
        1 ctaaatgaat tgttgttaga cgaagacttt ttcgccttta ccggatcgat gccagccgag
       61 atgtctttcc tcgcagtcca tgctttatcc cttgcctcct gcaaagtcat aagcggatat
      121 tttccgacgg tcaggatttt ctccttaccg tcaatcttgt agcgaagctg ccataccttt
      181 ttcccggata cagggacata aaggtacagg ccattaccat cgagaaggcg gtatggtttt
      241 tctttcggct ttgctgcttc aatctgctta acggtgagca tgggtaaaaa tccggtgggt
      301 aaaattattt tatccacttt ttacccgtca tggagtgcgg ctgtcaacga tctgacgcga
      361 accatgacga actgtgaata tacggaaggc ttgatattca ggggattttg cggactggta
      421 cggatgggag cgaactgata aatggtgtcc cctgcaggaa tcgaacctgc aattagccct
      481 taggaggggc tcgttatatc catttaacta agaggacaat gcggcgagta tacccgctaa
      541 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
      601 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
      661 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
      721 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      781 tgcagcgtac cgttaaacac cagatagagg atttcaacag aaccgaccac gatatgggtg
      841 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcacct
      901 gctgcaggaa acatccatac catagtggcg atcagccagc cggaaatgat cgcgttggca
      961 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     1021 gtttcttcat tgaagatagg catatattca aatgcccacg cagcaatacc agtcccgaga
     1081 atattaccca gcagcacgac tccccataac cgcataagta agccaacgtt gctcattgtc
     1141 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     1201 gccattatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
     1261 ggcacacctt ccagttcggc atgaaatatc ccttttgcca gtaacgaagc gcccatcgac
     1321 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttctag ctctttttca
     1381 ccatcctggc ggatatgctc atgaattgcc atcgctcggg aggggagtcg gtcttcatct
     1441 atttctattt ttttgccgcg ctctttttct tcgctctaaa cttcaatttc gtcgctgtgt
     1501 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1561 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt
     1621 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1681 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1741 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1801 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     1861 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     1921 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     1981 cgctgtcgcc tggcgtgatt atgttccgca accggcgcgt aatggtttga gcaactttac
     2041 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     2101 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     2161 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     2221 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     2281 cttcacgctg cgtgatgacg gtggtgatat ggcggatgct ctttacccgg ttctttcctg
     2341 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     2401 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2461 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2521 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2581 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     2641 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga tgtttgtcac
     2701 tgactatttc cgatattcag gcgttgaaaa atcaaaaaac tggaaaaata attttaaata
     2761 tcatcaaaat taacacctag tgcagatagt tgattaattt cattttgata tttcgtctgg
     2821 cgattttgaa gtcttttttc aactttatct tcgagattct taaagtcttt gtatttattt
     2881 tcaggatggt caacgtcaac tttgttagcg aataaagtag aaattatttc ttttacatta
     2941 tcgcgtctaa attttgcatc gtccacttga aaattatatc ttattgctcg acctaaacgt
     3001 ttaaaaactt cgttaggttc tctcagtctt tccccgttga gctcgtttac aacctgatga
     3061 tcggaaccag ttgcaaatga ttctgccatg atagctttac tgctttcaaa ccacgctgaa
     3121 gcactcctgg cgttttcact acaatattgg gtgagactat caccattaat acgaattcct
     3181 ctttgaatgt ggtagtcgtc ctgatcgtag taaccgagag ataagtaatc atttccgttt
     3241 ttatttttaa ttacaatagc gagagagtcg ccttttttat ctttaccgta ccaggtaaat
     3301 aagtcagttg gttcgctttt cccgctctca tcaaattccg ttaccgtatg tctgagtttg
     3361 tgtgatatct tatcgaagat aaaaccagga tctccatcat atatttcttt taatgcgctg
     3421 aagatcattt ccttgttatt gatatcatga gtcttctcaa ctatgttgtc attaatgata
     3481 gtacggacga gttctttgtc tttatagttc gcaggtaatt ctattggttc ttggttgtcg
     3541 ataccaatag taatatggtc tgtatcattg gagccaatat caatataagt aactgaggaa
     3601 ttattaatat tagatctata aggatcgtcg tttactcgta tggcgttttt tataagctca
     3661 agtagatgtt taatacaggt gcttattcct tccttaccat taaaaaggtt tttgaactct
     3721 tgtactaatc cattaatttc atcattgtta ttttcattga tggattctgg gtaagctgcg
     3781 ctggtattgt tgattgaaat agatggtagc atgattttat tccatgttga ttgatttgtt
     3841 acaaatatat attacctaat gaaatagact atcaaaaaac gctcatttat ttagaaccta
     3901 tctttaattt gagtgtataa gcgcacaata gccagggggg ttgagtatcc tggaaatgca
     3961 gaaaagtaaa ctctttgaga aggacgagca gaacgcttga aaatatgcat ttcccttcag
     4021 gtttgtctgt ggataccact tactgatttg aaagggcaag ctgccatcag gctgtttgca
     4081 aaactcagga ggcaagcaat gaggaaacta ctttcggtga agtgggggag ttaaagtcac
     4141 ctgcatagca ggtgacttta tccaggcgaa cgcgttatca gaacgcgtag ttaaagttag
     4201 taccgaacag ccaggcttta ccttcagact cgaactggta tgggccttcg ttaattttca
     4261 cgctctgacc gtgcatataa gaaacaccaa cgtcgactga agcatcttta ttaaatgcgt
     4321 aagtcgtacc tgcactcagc cagaaacggt cctggtccgg aatggagata gaacgattct
     4381 gagccggaac tgggctgtca tcaaaggcga taccggtacg gaaggtccag ttatcatcgt
     4441 agtaataagt ggtacccaac gcgatgcggt aagcatcttt aaagccttca tgtttctgga
     4501 acagcgtgtc gccactggtt gaggtcgctt tcagctgctg gaactgactc cagctggtgt
     4561 aagccaggct atagtgaatc gcccactgtg gatcaacacg gttataacct gacacttccc
     4621 acatttcagg caggttcagc gtcagataac ccgattgcgt tgcgccacct gtcgcggtag
     4681 gaattggcaa accgtagtta ttaaacgcac gattaagatc gctgctgtag ttacctttga
     4741 agtcaatttt cacttcagaa cggtaggtca gtgcatagcg gttattttta tccagttcat
     4801 acaggattcc ggcgttccag ccaaagcccc actggttacc gttcagatga gcgattttgg
     4861 tattactgtc gataccgttg gcggtagctg ccaatgcttg cccttgagga gtttgcccgg
     4921 caggagattg cataatctga ccagcaacca actgccccag atcgcctgcg aaacgttcaa
     4981 ttttcgcgcg agcgtagatg gcgttgaaac caagaccaaa gctccatgca ttatttaagc
     5041 gatacgcacc gcttaagttc aggttcatgg tttcaaggtc agttgtaccc ccgacagagc
     5101 cgcctgcata agtatcatta aactctgttg ccagaccata gttagaggta atagaagcgc
     5161 cccaaccaaa ttggtcgtta atcggtgcaa caaagtgcat gttcggaacc catgccgtag
     5221 gcgcgatgtt atcggctttc aggctacgac cagacggaga cgttccgctg atattcacat
     5281 ccgggtcaat ataaaccgca cccgcagaaa atgtcgggcg gtcaaacatg gtaatcaatg
     5341 cggggttacg gctaacgtta cctgcatcat cggcaattgc gccttcccct gaataagccc
     5401 ggcccaggcc agaggaagaa aattcgttta actgaaagcc tgccgaccag gcctgggtgg
     5461 agataagtgc cactgcgact gcgagagcag actttgtaaa cagggttttc tggctcatga
     5521 ccataacctc aatgatttat ttttatacaa actatgttac gtgctgtaac aggagcgcga
     5581 agtgtagggt ctgaggtaca tcttagaaat cagaccagtg gcgagagtat aggtcggacc
     5641 agctagaatg ttgcaagtat gtttccatct tttttcaaat atgatctaag aaacgcgatc
     5701 cagctggcaa aatttggagg ttgcgcactc gcggattcgg gtgaattttg ttttagatca
     5761 tttttaagtg tgatttcggt cacttatccg attttgcaga attaacaact ggagcagcac
     5821 tttccggaag cgtaaaatat ccacaacgtt tatctggcac cttgggtgca catacagagg
     5881 aaatcaacga tgagtaaatg tagtgctgat gaaaccccgg tttgctgctg tatggatgtt
     5941 ggcaccatta tggacaactc cgactgcacc gcgtcttaca gccgcgtgtt tgcaaaccgc
     6001 gcagaagcag aacaaacgct ggcggcgctg actgaaaaat cccgtagcgt gaaatccgaa
     6061 ccgtgcaaaa tctccccgac tttcactgaa gagtctgacg gtgtgcgcct ggatatcgat
     6121 ttcacttttg cctgcgaagc tgaaatgctg atcttccagc tgggtctgcg ttaattcctc
     6181 tcgggtacgt acgcccctgt cagttgctgg caggggcgtt ttcttttctt tcttctgtct
     6241 tcactgtgtc atagctccca ctttcctttt tctccgcttg gctaaatgta aaaaaatggt
     6301 taagactatg atcaggtcag accactttat ttattttttt acaggggagt gttatgggtc
     6361 aggttttacc actggttacc cgccagggcg atcgtatcgc cattgttagc ggtttacgta
     6421 cgccttttgc ccgccaggcg acggcttttc atggcattcc cacggttgat ttagggaaga
     6481 tggtggtagg cgaactgctg gcacgcagcg agatccccgc tgaagtgatt gaacagctgg
     6541 tctttggtca ggtcgtacaa atgcctgaag cccccaacat tgcgcgtgaa attgttctcg
     6601 gtaccggaat gaatgtgcat accgatgctt acagcgtcag ccgcgcttgc gctaccagtt
     6661 tccaagcggt tgcaaacgtc gcagaaagcc tgatggcggg aactatccga gcggggattg
     6721 ccggtggggc agattcctct tccgtattgc caattggcgt cagtaaaaaa ctggcgtacg
     6781 tgctggttga tgtcaacaaa gctcgaacca tgagccagcg cctgaaactc ttctctcgcc
     6841 tgcgtttgcg cgacttaatg cccgtaccgc ctgcggtagc agaatattct actggcttgc
     6901 ggatgggcga caccgccgaa caaatggcga aaacctacgg catcacccga gaacagcaag
     6961 atgcattagc gcaccgttcg catcagcgtg ccgctcaggc atggtcagac ggaaaactca
     7021 aagaagaggt gatgactgcc tttatccctc cttataaaca accgcttgtc gaagacaaca
     7081 atattcgcgg taattcctcg cttgccgatt acgcaaagct gcgcccggcg tttgatcgca
     7141 aacacggaac ggtaacggcg gcaaacagta cgccgctgac cgatggcgcg gcagcggtga
     7201 tcctgatgac tgaatcccgg gcgaaagaat tagggctggt gccgctgggg tatctgcgca
     7261 gctacgcatt tactgcgatt gatgtctggc aggacatgtt gctcggtcca gcctggtcaa
     7321 caccgctggc gctggaacgt gccggtttga cgatgagcga actgacattg atcgatatgc
     7381 acgaagcctt tgcagctcag acactggcga atattcagtt gctgggtagt gaacgttttg
     7441 ctcgtgaagt actggggcgt gcacatgcca ctggcgaagt ggacgatagc aaatttaacg
     7501 tgcttggcgg ttcgattgct tacggacatc ccttcgcggc gaccggcgcg cggatgatta
     7561 cccagacatt gcatgaactt cgccgtcgcg gcggtggatt tggtttagtt accgcctgtg
     7621 ctgccggtgg gcttggcgcg gcaatggttc tggaggcgga ataatggaaa tggcatcagc
     7681 gtttactctt aatgttcgtc tggacaacat tgctatcatc accatcgacg taccggatga
     7741 gaaaatgaat accctgaagg cggaatttgc ctcgcaggtg cgcgccatta ttaagcaact
     7801 ccgtgaaaac aaagagttgc gcggcgtggt gtttatctcc gcgaaaccgg acaattttat
     7861 cgccggagca gacatcaaca tgattggcaa ctgcaaaacg gcgcaagaag cggaagcact
     7921 ggcgcggcag ggccaacagt tgatggcgga gattcatgct ttgcccattc cggttatcgc
     7981 ggctattcat ggcgcttgcc tgggcggtgg gctggagctg gcgctggcgt gccacggtcg
     8041 cgtttgtact gacgatccta aaacggtgct cggtttgcct gaagtacaac ttggattgtt
     8101 acccggttca ggcggcaccc agcgtttacc gcgtctgata ggcgtcagta cagcattaga
     8161 gatgattctc accggaaaac aacttcgggc gaaacaggca ttaaagctgg ggctggtgga
     8221 tgacgttgtt ccgcactcca ttttgctgga agccgctgtt gagctggcga agaaggatcg
     8281 cccatcttcc cgccctctac ctgtacgcga gcgtattctg gcggggccgt taggtcgtgc
     8341 gctgctgttc aaaatggtcg gcaagaaaac agaacacaaa actcaaggca attatccggc
     8401 gacagaacgc atcctggagg ttgttgaaac gggattagcg cagggcacca gcagcggcta
     8461 tgacgctgaa gctcgcgcct ttggcgaact ggcgatgacg ccgcaatcgc aagctttgcg
     8521 tagtatcttt tttgccagta cggacgtgaa gaaagatccc ggtagtgatg caccgcctgc
     8581 gccattaaac agcgtgggaa ttttaggtgg tggcttgatg ggcggcggta ttgcttatgt
     8641 cactgcttgt aaagcggggc ttccggtcag aattaaagat atcaacccgc agggcataaa
     8701 tcatgcgctg aagtacagtt gggatcagct ggagggcaaa gttcgccgtc gtcatctcaa
     8761 agccagcgaa cgtgacaaac agctggcatt aatctccgga acgacggact atcgcggctt
     8821 tgcccatcgc gatctgatta ttgaagcggt gtttgaaaat ctcgaattaa aacaacagat
     8881 ggtggcggaa gtggagcaaa attgcgccgc tcataccatt tttgcttcga acacgtcatc
     8941 tttaccgatt ggtgatatcg ccgctcacgc cgcgcgacct gagcaagtta ttggcctgca
     9001 tttcttcagt ccggtggaaa aaatgccgct ggtagagatt attcctcatg cgggtacatc
     9061 ggcgcaaacc atcgccacta cggtaaaact ggcgaaaaaa cagggcaaaa cgcccattgt
     9121 cgtgcgtgac aaagccgggt tttacgtcaa tcgcatctta gcgccttaca ttaatgaagc
     9181 tatccgcatg ctgaccgaag gtgaacgggt agagcacatt gatgccgcgc tagtgaaatt
     9241 tggttttccg gtaggcccaa tccaactttt ggatgaggta ggaatcgaca ccgggactaa
     9301 aattattcct gtactggaag ccgcttatgg agaacgtttt agcgcgcctg caaatgttgt
     9361 ttcttcaatt ttgaacgacg atcgcaaagg cagaaaaaat ggtcggggtt tctatcttta
     9421 tggtcagaaa gggcgtaaaa gcaaaaaaca ggtcgatcct gccatttacc cgctcattgg
     9481 cgcacaaggg caggggcgac tctccgcacc gcaggttgct gaacggtgtg tgatgttgat
     9541 gctgaatgaa gcagtacgtt gtgttgatga gcaggttatc cgtagcgtgc gtgacgggga
     9601 tattggtgcg gtatttggca ttggttttcc gccatttctc ggtggaccgt tccgctatat
     9661 cgattctctc ggcgcgggcg aagtggttgc aataatgcaa cgactggcca cgcagtatgg
     9721 ttcccgtttt accccttgcg agcgtttggt cgagatgggc gcgcgtgggg aaagtttttg
     9781 gaaaacaact gcaactgacc tgcaataaga aggtcaaagc tatatgaatc cgcgctgaat
     9841 ggcggagtgt tggtcaaaat gtaaacgcat attgactata cttacgccat tgaggtaaaa
     9901 aacagcgttt cattcggtga atggataagg cacaatgccg gccaccgttt tctttctctg
     9961 gtttcagatg aaagaaaacg ggcgaatctg gttaacaaaa gcggtgcaat atgcaagttt
    10021 ttatcatgcg tcacggcgac gcagccctcg attccgccag tgattcggtt cgtcctctga
    10081 ccactaatgg ttgtgacgaa tctcgcctga tggcgaactg gctgaaaggt caaaaagtgg
    10141 aaatcgaacg cgttctggtg agcccgttcc tgcgagccga gcaaacactg gaagaagtag
    10201 gggattgtct gaacctgcct tccagtgcag aagttctgcc ggagttaacg ccctgcggcg
    10261 atgttggtct ggtcagtgcc tatttgcagg cgttgaccaa tgaaggtgtt gcttcggtac
    10321 tggtgatctc tcacttaccg ttagtcgggt atctggttgc cgagttatgt ccgggcgaaa
    10381 cgccgcctat gtttaccacc tcggccattg ccagcgtaac gcttgatgaa agcggaaacg
    10441 gtacattcaa ctggcagatg agcccgtgta atctgaagat ggcaaaagct atctgatgtg
    10501 ttccaaatta tgcccaatga ggtagattca ttgggcataa ttgctcatta ggcagcggat
    10561 atctgtcaac ctcactacta tcgaag
//
LOCUS       DAJTYH010000126        10421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTYH010000126 DAJTYH010000000
VERSION     DAJTYH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..341
                     /locus_tag="OO609_003399"
     CDS             <1..341
                     /locus_tag="OO609_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6634697.1"
                     /translation="YEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQ
                     PVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALAK
                     VIPFFNTHLG"
     gene            359..997
                     /locus_tag="OO609_003400"
     CDS             359..997
                     /locus_tag="OO609_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6634698.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1288..2016
                     /gene="mpaA"
                     /locus_tag="OO609_003401"
     CDS             1288..2016
                     /gene="mpaA"
                     /locus_tag="OO609_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6634699.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1991..2956)
                     /gene="ycjG"
                     /locus_tag="OO609_003402"
     CDS             complement(1991..2956)
                     /gene="ycjG"
                     /locus_tag="OO609_003402"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6634700.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3075..3581
                     /gene="tpx"
                     /locus_tag="OO609_003403"
     CDS             3075..3581
                     /gene="tpx"
                     /locus_tag="OO609_003403"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6634701.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3625..5166)
                     /gene="tyrR"
                     /locus_tag="OO609_003404"
     CDS             complement(3625..5166)
                     /gene="tyrR"
                     /locus_tag="OO609_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6634702.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5314..6375)
                     /locus_tag="OO609_003405"
     CDS             complement(5314..6375)
                     /locus_tag="OO609_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6634703.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6372..7769)
                     /locus_tag="OO609_003406"
     CDS             complement(6372..7769)
                     /locus_tag="OO609_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6634704.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNASFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7925..8923
                     /locus_tag="OO609_003407"
     CDS             7925..8923
                     /locus_tag="OO609_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6634705.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9034..9906)
                     /gene="ompG"
                     /locus_tag="OO609_003408"
                     /pseudo
     CDS             complement(9034..9906)
                     /gene="ompG"
                     /locus_tag="OO609_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(9979..10421)
                     /locus_tag="OO609_003409"
                     /pseudo
     CDS             complement(9979..10421)
                     /locus_tag="OO609_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         2619 a         2791 c         2454 g         2557 t
ORIGIN      
        1 tttatgaagc aactgactac tataaaacac cacgcgggca acaaccggga ggtgcgacaa
       61 aaatgttgtt ctcacatgca cagaaaacgc tggcctggga tgccttcgca tttactgaag
      121 tgttactgac ccagccggtg atggtagtag ttggtgaaaa agtcggtgcc ttcggtgctt
      181 atcgtgatgg actggaagtc tatggccggg cgatggtttc gcaggatcgt cagttagttt
      241 cgttacccga tttctcacat tatgaactat atgacaaacc ggaagcagtt caggaggcgc
      301 tggcaaaagt tattcctttt ttcaacaccc atctgggata agtttcatga gaaatattat
      361 gaaaaatgta ctgattcttg gtgctggcgg tcagatagcc cgccatgtga ttaatcaact
      421 ggcagataaa caaactatta aacaaacttt atttgcccgt cagccagcaa aaatacacaa
      481 accctatcca actaatagcc agattattat gggggatgta ctgaatcatg cagcactgaa
      541 gcaggccatg cagggacagg atattgtgta tgccaacctt acgggggaag atttagatat
      601 tcaggcaaat tcagtgatcg ctgcgatgaa agcctgtgat gttaaacgtc tgatctttgt
      661 tctttccctc ggtatttatg atgaagttcc aggaaaattt gtcgaatgga ataatgcagt
      721 tattggtgaa cccctaaaac cattccgcag ggctgcggat gcaattgagg cttctggact
      781 ggaatataca attcttcgcc ctgcctggct gactgatgaa gatattattg attatgagct
      841 tacctctcgc aatgaaccat tcaaaggtac gatcgtatca cgtaagagtg tcgcagcatt
      901 aattacagat atcatcgata aacctgagaa acacattggt gagaatatag gcatcaatca
      961 acccgggact gatggcgata agccattctt catgtaaatc aattatatag ataccatcgt
     1021 actgtactat gtgcagtacg atttattaaa cttattaagg tgaactgatc aatcagatgc
     1081 gattaacatg tgtagaacca gtaagaataa aatacgccat gtatataaca ttacgacaat
     1141 tctaatatct gcacatcaat gaaaatgcaa acaacttccg gcaaaaaagt gatacaagat
     1201 cgatgacata gcgttgaaga aaaggatttg ataatccgca aaatttgcac aacactgcct
     1261 gtaatacttc ctgaaccaga gacgatcatg accgtaaccc gctcacgcgc cgaacgcggc
     1321 gcatttccgc ccggaacaga acattacgga cgttcattat tgggcgcgcc gttaatctgg
     1381 tttcctgccc ctgcagccag ccgcgaaagt ggtttgattc tggctggcac tcacggtgat
     1441 gaaaactctt cggtcgtcac gctctcctgt gctctgcgga ctttgacacc ttctttgcgc
     1501 cgccatcatg tggtgctgtg tgtgaatcct gacggctgcc agctggggtt acgggccaat
     1561 gctaatggtg tggatttaaa cagaaacttt ccggcggcga actggaagga aggtgaaacg
     1621 gtttatcgct ggaacagcgc cgctgaagaa cgtgatgtcg tattactgac aggtgacaaa
     1681 cctggctccg aacctgaaac ccaggcgctg tgccagctta tacaccgcat tcaacctgcc
     1741 tgggtggtct cattccacga tccactggct tgtattgaag atcccagaca caacgaatta
     1801 ggcgaatggc tggcccagtc gtttgaattg cctctggtaa ccagcgtcgg ttatgaaacg
     1861 cctggctctt tcggcagttg gtgtgcagat ctcaacttgc attgcatcac cgcagagttt
     1921 ccgcccatct cctccgacga agccagcgaa aaatatctct ttgcgatggc aaacttgctg
     1981 cgctggcatc ctaaagatgc aattcgcccg tcgtgaactg aagcgccggt tccacatcta
     2041 ccgccagcca ggtcggtccg tcaagatcgg cgaaactgac ctgcggcacc agcggtaaag
     2101 cggcgctaat ggcacgagag gtacacaaca tgcagcccag catcagactg aaaccttgtg
     2161 cacgcgcttc agtcgccagc gccagcgctt ccgtcagacc accggtttta tcgagcttaa
     2221 tgttaaccat ctcatagcgc cctttcagcg ccttcagatt gctacgagta tgacaacttt
     2281 catcagcaca aatcggcaac ggatgaataa aattctccag cgccgcatcg tcctgcgcag
     2341 gaagcggttg ttcaagcatc gcaacgccta aatccgccaa tagctggcaa cgcgccgcca
     2401 acccttctgc acgccaggat tcatttgcat caacgatcag cgtggcatcg ggcacagcgg
     2461 tgcgaattgc caccatccgc tcactgataa gatggttatc cagcttcact ttcagtaatt
     2521 tcgcgcctgc ctgccagagt gttgatgcac tattggccat ctgatcaggc gtaccgatga
     2581 caaccgtctg tgcagtgata actgtctcgg gaagcgttat gccgatcaaa tcagccagcg
     2641 attgctgctg ttttcgcgcc gcgagatccc acaatgcaca atccagcgca ttacgtgctg
     2701 cgccggcagg gagaattttt tgcaactcct cccgtgtcag ccctttctct aattgcggca
     2761 cgacgctcat aatttgcgcc attaccgagg catcactttc tccataacgc ggatacggcg
     2821 tgcattcgcc ggtgccttta ataccctctt cttccagttc aaccaccacc acgcgcgctt
     2881 cactgcgact tccccgggca atcacaaacg gggtatgtaa gggccaggct tcctcaaata
     2941 ccttaacgat tctcatcttt actccttgtt gtcgcggtgg tctgcggatt aagcgcaaat
     3001 agcgtttgct gtgttattga cagttagcat aaactaggtg tgacgttaac tatatgtaaa
     3061 caggaaagat aatcatgtca caaaccgttc atttccaggg caacccggtt acagtcgcca
     3121 attccatccc gcaggcgggt agcaaagcgc agacttttac tctcgtggca aaagatctgt
     3181 ctgacgtcac cctcggtcag tttgcgggta aacgcaaagt gctgaacatt ttcccgagta
     3241 ttgataccgg tgtttgcgcc gcatcagtac gtaagtttaa ccaactggca actgagatcg
     3301 acaacaccgt tgtgctgtgt atctctgccg atctgccgtt cgcccagtct cgtttctgcg
     3361 gcgcagaagg tctgaacaac gttatcaccc tctccacttt ccgtaacgct gaatttctgc
     3421 aagcctacgg tgtggcaatt gctgatggcc cactgaaagg tctggcagcg cgtgccgttg
     3481 tggttattga cgaaaatgac aatgtgattt tcagccagct ggtggatgaa atcaccaccg
     3541 agccggatta cgaagcagct ctggctgtac tgaaagcata agttaatatg cctgatggtg
     3601 ttgcaccatc aggcatattc gcgcttactc ttcgttcttc ttctgactca gaccatattc
     3661 ccgcaactta ttggcaatcg cggtatgtga aacgccgaga cgttttgcca gtttgcgcgt
     3721 gctgggataa ttgcgataaa gctgggttaa taccgagcgt tcaaaacggc tggtgatttc
     3781 gtccagcgaa ccttccatcg catcttcgcc cacggctacc gttgcggcgt cataatccgg
     3841 caacaaaata tcctgtggac gcagctcata accgtccagt tgtgtcagtg cgcgatagat
     3901 agcgttcttt aactgccgca catttcccgg ccacgcataa cgtgtaagta cagtattcag
     3961 gtcagcggcc agtttcggac gcggcacgcc ctgctcgtcg gcaaagcggg cgacgaacag
     4021 ctcagttaac ggcataatgt cctgcggaca gtcacgtagc ggcggcagat tgagcgtcaa
     4081 cacgttcaga cgataataga gatcttcacg gaacacgcct ttttgcacca gttcgaccag
     4141 attcttctgc gtagcgcaaa tcacccgcac atcgacatgc acctcatggt cttcgccaac
     4201 ccgacggaaa gtgccatcat taagaaaacg cagtaatttc gcctgcatcc gtggtgacat
     4261 ttcccctatt tcatccaaca gcaccgaacc accgttcgcc tgctcaaaga accctttctt
     4321 cccttccgga gcatgaccaa acagttcact ctcgaccgca tcttctggta tagacgcgca
     4381 attcagcgcc aggtaaggtt tgcccgctct ggggcttgcc tgatggcagg cgtaggcaaa
     4441 aagatcttta cctgtacctg tgtcacccgt aatcagcagc ggcgcgctta gcatcgccag
     4501 tttctgcgcc tgttcgacaa cgtgcttcat tttcgggctg acggcgacaa tttggctgaa
     4561 ggcgctgacg tcctgggcgg cgacattttg caactggcgg cccatacgaa tcgttgatcg
     4621 caacatcacc accgcaccgg tcaggacgtg ttgatcattt tcatcctgaa gataaacagg
     4681 cgtaatctcc atcaggaaat tctgcccatt aataacgaca tgctcgttat gcgaatcttg
     4741 cggttcgctt tccagccaac gtaaaaaatt aaagccgtta atcaattgtg cggcggtatg
     4801 gttgcgcagg cgatccaatt tttgcccaaa aagctgacag ctcgccgggt tcgccatatc
     4861 cactttgctt ttcatatcga cagagagcac aggttcaggc aacgcctcca gtaacgcgct
     4921 caacgccaga tgctcacgtt cggaaggcat ccacgggaca gtacgcacat cggtaacacc
     4981 tgcaatacgg cgtatttcgg ccatcagact gctgaaactc tcaaactcca gttcagcaaa
     5041 attgaggtag attcgcccaa tgggatcaat ctcaataccg cgtaaatcaa tgcctcttag
     5101 cacgagtaga tcgagtaatt cgcgggtcag accgagtcgg tcttcacaaa agacttccag
     5161 acgcatggga accttcacct gaaaaaagaa caattaatat gatgatatga cactatttgg
     5221 atagcaggaa ggtttctgtc aacaatcatt gacacaaaga cggaaaaacg ggcacttcgg
     5281 cgtaaagata tccggtcagc acccgatatt gcattatttt tcgctgggcg ttttgccttt
     5341 ttgcagagtt tctttcacct gaccgataag ctgacgacgg aaatccccaa ggcgaggttt
     5401 gtcatcgtca atccacggca gcgggcggca aagctccata gctttaatcc cgagtcgtac
     5461 cgtcagaagt ccagcaccaa ttccctgagc tgcgcgggta gacaaacgag cagcgagatc
     5521 ttgcgacatc cagtccatcc ccacttcgcg caccagttcg ctggctccgg caaaagcgat
     5581 attcagcaat accagcttaa acagacgcaa acggctgtaa taccccagtt caatgccata
     5641 cagcgtggca atgcgattaa ttaaacgcag attgcgccag gcgataaacg ccatatcgac
     5701 caacgccagc gggctgaccg caatcatcaa cgttgattcc gccgccgaac ggctgatttc
     5761 gcgccgcgcc tgggcatcta aaactggctg gaccaaatgc gcatacaggc tgaccacttc
     5821 acgatcgttc tgcgtttcat ggattgaggc ataccagcgt tgcagcgccg gatgcgactg
     5881 atcaataccc gcctgctgcg ccagtttttc gcaaaatgcg cgacctttgc ccgtgccgtg
     5941 gctatgcaat aaatcacgcg cttcgtcgcg ttcatgggcg cgctgtcgca agcgccataa
     6001 gcgccgccac tctgttacca cagaacctac gccagcgccg ataatcaatg ccccagcggc
     6061 acatccaccc agcgctaccc agtcctgggt ttgccaggca ttcattgtcc actgtatacc
     6121 ctgcccgaca acgcttgcgc caaacagagc cagcccgccc atcaccattt tgcgccacag
     6181 gctgcgtttc ggacgtaacg ctgcgtccat taccgcttcg acttgcccct cttcttcctg
     6241 cgcttcgtcg agcgtggccg gggcaaaatt ttgcgcctga ttttcgtcaa aggtctgctg
     6301 cgccctgaat tttggattct gttcgacctc cagcggaccg tcgaaatcaa tacgtggttt
     6361 taacggttcg gtcatcgcaa tttatctcct attaaaaatt ccagcgcggc atcaagacga
     6421 atatgcggca gcggtttgtc gacatccatc acctgcgggc gaaacgcttc aaactggaac
     6481 ccttgcttgt cccagaacgc ctgaccgggc aaacgcgcag gaacttcacc ggggtaaacg
     6541 gtgagaagcg caccatcgct gagccggtta ccgcgcaatg ccggaatttt ctcgccgttg
     6601 acgtcaataa tgccgctggt ggtcgcctgc accgaggcca gccccaggca atccatacta
     6661 atcctttcaa acgacgcatt ttgccaggca tcctgaatca gttgttgcag taatgaaacc
     6721 atattggcgt gctgatcgac cgttacatgg tccgctttca tcgcggcaaa cagcagtttg
     6781 tcgataaccg gcgagaataa gcggcgaaac agcgtgcgct gcccgtagtg gaaactttgc
     6841 atcagctgcg tcagtgccag acgcatatca ttaaatgcct gtggcccact gttgagaggt
     6901 tgcaggcaat ccaccagcac aatctggcgg tcaaagcgca gaaaatgatt cttatagaac
     6961 cccttcacca ctttctcgca gtaataatta aaccgctcgc gcagcattcc ggcattggtg
     7021 tgcttatcgg cctgcgccag tttggactcg tcccaggcat cgacatccgg ccacgggaag
     7081 aattgcagcg cgggcgcacc tgccatatct cccggcaaga caaagcgccc aggctgaata
     7141 aagtgcagcc cctgctgttt acagtggtgg agataatcgg tccacacggc ggcaatgtct
     7201 gccagacggt tttcgtcggc aggcgctagc gggtcaagtc cttcgcacat cattcgccat
     7261 ttcgccgacc attcgccgcg ctgaccattg agtaagcccg tcatctggcg cgaccagctt
     7321 aagtaatcct gcgccagcat cggcaggtcg agcaaccatt cgccgggata atccacaatt
     7381 tccagataca gcgtggaggt atccttaaag tggcgcagca gcgcatcgtt cgatttaaaa
     7441 cgtaacgcca ggcgaatttc actgacgccg cgcgtaggcg tcggccaggc gggaggatca
     7501 ccatataact gcgccagtcc ttcatcatag gtgaagcgcg gaatgccaaa gtcacgctgg
     7561 ggaatgcgtt ttacgcccag caagcgctct tcacgcaccg cgcttaacag cggcaaacgt
     7621 gctccggcat gaatattgag caactgatta accatcgcgg tgataaacgc tgttttgccg
     7681 ctgcggctaa gtccggttac agcgaggcgc agatgtctgt cgacaccccg attcaccagc
     7741 gcattgagtt catttttaag tcgcttcatt gctgtcctgt gctgctctga agaccagaaa
     7801 aaaaatcatt cgtaaggata tgaatgtggg ggccaggcca ttgattatca actgcccgtg
     7861 ttgtcatcga cgggcgaact ccataagatg tgcagcattc acgttcggac ggagttcctg
     7921 acctatgtcc cctactattt atgatattgc cagggttgca ggcgtatcaa aatccaccgt
     7981 atcacgcgtg ctgaataagc aaactaatat ctccccggaa gcgcgcgaaa aagtgttacg
     8041 ggccattgaa gaattacagt atcagccaaa caagctggcc cgcgcgctga cctcttcggg
     8101 ttttgatgcc attatggtga tttctacccg ttcgaccaaa actacggcgg gtaatccgtt
     8161 tttctctgaa gttttgcatg ccatcaccgc caaagcggaa gaagaaggtt tcgacgtgat
     8221 attacaaacg tcgcacaatc cggcagaaga tctgcaaaaa tgcgaaagca aaattaaaca
     8281 gaaaatgatt aaaggcatta ttatgctcag ttcgccagcg gatgagtcat tttttgccca
     8341 actcgataaa tacgatattc cggtggtggt aattggcaaa gttgaaggtc aatatgccca
     8401 tgtttattct gtcgataccg ataattttgg tgacagcatt gcgttgaccg atgcattaat
     8461 tgaaagtggg catcaaaata ttgcctgcct gcatgctccg cttgatgtcc atgtttcggt
     8521 tgatcgcgtt aatggctata agcaaagtct gtccgcgcat aatattgcag tgcgtgataa
     8581 atggattgtt gacggcggtt atacccatga aacagcactg caagcagcac ggcaattatt
     8641 aagtcagtca ccgttgcctg aggcagtgtt tgccactgac agcctgaaat taatgagcat
     8701 ttatcgtgcg gcggcagaga aaaatattgc tattccgcag cagttagcgg tggtgggtta
     8761 tagtaatgaa acgctgtcgt ttattttaac gcctgcacca ggcggcatcg atgttccgac
     8821 gcaggagtta ggtcaacaaa gctgcgagtt attattccgc ttaatttcgg gaaaaccgtc
     8881 accacagaat attaccgttg ccacgcatat gacgctgaaa taaataccag ctaccttgcc
     8941 tgaatgccgg atgcgatgct ggcgcatctt atccggccta catttataga tcggataaag
     9001 tactcgcgcc gcatccggca atttagccca ttatcagaac gagtaattta cgccgacacc
     9061 tgcataatgg aatttatcgc tgtcgccttc gtcgtgatcc tgccactcaa acgcgtattc
     9121 cagcgaaacg gaaagtccgt tctggaaatc ataaccgtaa aataaaccca cacggttaaa
     9181 atcatggcct tcacgttcaa tatcatcctg ccagtcccag ttactctagc gatccagccc
     9241 aatgcgcgta tacggcgtca ccgagtggtt gccgagcgtc agcggcaaat aggcgcgaat
     9301 ttcttgtgtg gaaaactcac cgttattgcg gctgtcgtcc atattgaagc cgcgctcgag
     9361 ataatagttc actcgcaaag caaccgtttc gttgaaggta tattgcagac ctgtttccgt
     9421 ttcgacacgg gtatcagcgt aaccggtcgt gttcaaatcg ttggcaaatt tatacatcga
     9481 caaccaaccg ttgaaacgta aatcatcagt cagtttcaca tcccagtctg gcgcgatttt
     9541 ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt
     9601 acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac
     9661 ctccagctcc gggcgatcaa acctcgttcc acgtttaccc gcactataat ctaccggccc
     9721 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
     9781 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
     9841 cgcaccgata ttaaagtgcc agtcgctctt ttcctcggcc tgtgcgcagg ccattcccgc
     9901 acacatcacc agtgcggtac agggtaataa ctttttttgt tccttgtatt atcctggggg
     9961 aatgccttcc ccctgtattt agcgaattgc tatttccgtt tctgcatcaa agaaatgaca
    10021 tttcgtcata tcaaaatgaa tagtgatatt ttctcctgca tgataatcat ttaacgcacc
    10081 agcacggacc actaactcgt gcccccaacc gtggtgtaga gcataaattc cgcaccggtt
    10141 aattctgcca cgctaatttt ggcggaaatg ttattttcct cttgcgcgtc cggatgaata
    10201 tcttccgggc gtattcccat cacgatgggc ttatgcaaac tttcctgtgt tttcagaacc
    10261 gctaatttct cttcgagaat ggttaattta agcgtttccg taacgaattt atcgccatcg
    10321 atcgtgccgc gaataaaatt catcgctggt gatccaataa atccggcaac aaacatattc
    10381 gcgggttggt tataaacggt tttcggcgca cctacttgct g
//
LOCUS       DAJTYH010000127        10309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTYH010000127 DAJTYH010000000
VERSION     DAJTYH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1367
                     /gene="amn"
                     /locus_tag="OO609_003410"
     CDS             <1..1367
                     /gene="amn"
                     /locus_tag="OO609_003410"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6634706.1"
                     /translation="AIGKYITIGELPDENARKQGLFVYPSLTVTWDGSTTNPPKTRAF
                     GRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPYVIDGSEL
                     TLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSLARLRHYT
                     GTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWITAETEAPEE
                     AISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDIWLMIGHC
                     GGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGE
                     EVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVPYGT
                     LLCVSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDRLHSRKLRTFNE
                     PPFR"
     gene            1710..2426
                     /locus_tag="OO609_003411"
     CDS             1710..2426
                     /locus_tag="OO609_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6634707.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(2882..2957)
                     /locus_tag="OO609_003412"
     tRNA            complement(2882..2957)
                     /locus_tag="OO609_003412"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2922..2924),aa:Asn,seq:gtt)
     gene            complement(3058..4685)
                     /gene="yeeO"
                     /locus_tag="OO609_003413"
                     /pseudo
     CDS             complement(3058..4685)
                     /gene="yeeO"
                     /locus_tag="OO609_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4690..4765
                     /locus_tag="OO609_003414"
     tRNA            4690..4765
                     /locus_tag="OO609_003414"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4723..4725,aa:Asn,seq:gtt)
     gene            complement(4803..5753)
                     /gene="cbl"
                     /locus_tag="OO609_003415"
     CDS             complement(4803..5753)
                     /gene="cbl"
                     /locus_tag="OO609_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6634708.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5855..6772)
                     /gene="nac"
                     /locus_tag="OO609_003416"
     CDS             complement(5855..6772)
                     /gene="nac"
                     /locus_tag="OO609_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6634709.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7100..7175
                     /locus_tag="OO609_003417"
     tRNA            7100..7175
                     /locus_tag="OO609_003417"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7133..7135,aa:Asn,seq:gtt)
     gene            complement(7231..8166)
                     /gene="ldtA"
                     /locus_tag="OO609_003418"
     CDS             complement(7231..8166)
                     /gene="ldtA"
                     /locus_tag="OO609_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6634710.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8228..9307)
                     /gene="cobT"
                     /locus_tag="OO609_003419"
     CDS             complement(8228..9307)
                     /gene="cobT"
                     /locus_tag="OO609_003419"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6634711.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9319..10062)
                     /gene="cobS"
                     /locus_tag="OO609_003420"
     CDS             complement(9319..10062)
                     /gene="cobS"
                     /locus_tag="OO609_003420"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6634712.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10059..>10309)
                     /locus_tag="OO609_003421"
     CDS             complement(10059..>10309)
                     /locus_tag="OO609_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6634713.1"
                     /translation="PDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVP
                     ESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2892 a         2629 c         2218 g         2570 t
ORIGIN      
        1 acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc cgtaaacaag
       61 gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca aatcccccca
      121 aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg attactcgcc
      181 ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag gattatggtg
      241 cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc atcgatggct
      301 ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc ccgacaacag
      361 aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg gaattctccc
      421 cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg cgccattata
      481 ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac acacgttatg
      541 tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt ccctacattg
      601 ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca gaagaagcca
      661 tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt accgctgatg
      721 gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa accatctgcg
      781 atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt ggcggattac
      841 gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc gatgaccacg
      901 ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa gtgcaacgtg
      961 cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc aaacagcggc
     1021 tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt tactcagctt
     1081 ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt gcaaccattg
     1141 ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt tcagataaac
     1201 cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa ggcgctattt
     1261 ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa ggcgaccgac
     1321 tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa gttatgaacc
     1381 ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag cctgcaataa
     1441 acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta atgatttcca
     1501 catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc aacaacatta
     1561 gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc taatagacga
     1621 gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt taccagtaat
     1681 gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat attgttgcta
     1741 aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt gtagaaatct
     1801 atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta aaattcgtta
     1861 ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca attgataaag
     1921 ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt ggtcctaatg
     1981 gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt gctaacgttc
     2041 gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc agctatatgt
     2101 ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt tttgaaattt
     2161 tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat attgttattt
     2221 atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct ggaatcactg
     2281 agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt tccccagaag
     2341 atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat gtacaaaaag
     2401 tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt tatggcagat
     2461 ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca tattgcttat
     2521 aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt ctttttctga
     2581 ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga cagctacgtc
     2641 ccgcataagg catttttctc tttatctttg tacggtactt catggaacag aatttttgac
     2701 cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg tgagactcga
     2761 ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa gtggccgggt
     2821 aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca aaactaaaat
     2881 ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg ccgttctacc
     2941 gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc acccacaatg
     3001 tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt ttgtcgttta
     3061 ttccgacaca actggctttt tttcacactt ttgcggctcg gatcgagggt atttccacag
     3121 ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc agtcggcaaa
     3181 cattcccatc cagacaccaa ccacacccca gccaagcatg attccctgca cataaccgac
     3241 tacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac gggcatcacg
     3301 agcaccctta aatccagcgg gtagcaccca tgaggcggac caaataggca taaataaagc
     3361 atttagctaa atcagaatca caacgacgtg tttaacctgt ggatcctggg tgtaaaacga
     3421 tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc caagagtgga
     3481 cagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta cccccaacct
     3541 tcggcctgta atgatcgtag aagcagaacc gagcgcactt ccgggtaagt tgataagagc
     3601 cgcaattgag aacgcgataa aatttccggc aataacgctg gtccccatcc cggcaacgaa
     3661 catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg ctggaatacc
     3721 aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc tctttaacga
     3781 aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa ctgcgccaat
     3841 ataacgagaa atggttaaac ccaacccagc cccgaccagg agaaaaggcc gtaaatcaat
     3901 atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag cggtattttc
     3961 gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc ggctggataa
     4021 ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacttctgt cgtggcatca
     4081 cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc caacagtacg
     4141 gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc
     4201 cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc tgcaaaaaaa
     4261 gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc atcttttccc
     4321 agccaactga ccagaaaagt gctcaggacc cccatcaaca ggacacaggc gttctccatg
     4381 aagataggaa cagcaagcgg ggttatctcg cgctagaaca acactttgta gctcttgcgt
     4441 ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat attcaaagcc
     4501 gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc agcaaagctg
     4561 caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa
     4621 cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa gatgtgcctc
     4681 aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact
     4741 ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata gcgggctttt
     4801 tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg cttgatatcc
     4861 tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc aaacatagtt acgctgaagt
     4921 tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg cgtatccagg
     4981 cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa tccgatccca
     5041 agcccaagag caacataggt tttaatgaca tcagaatcct gtgcacttaa tacaatatct
     5101 gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg agcgccccgt aattccctgt
     5161 cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt aatttgcgtt
     5221 aagggatgat cgagtggaac aagcaaacta tggtgccaac aaaaccatgg gaaggcgacg
     5281 agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc gccattttgc
     5341 aacaacgtcg caatttcctg tggcgaaccc tggattagct cgagccgaac ctccgggaaa
     5401 agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt atgcgtcgtt
     5461 gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg acgaacatta
     5521 ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg ttcagtcatc
     5581 cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caaattcatc ctccagttcc
     5641 cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac ctctgtcagg
     5701 ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt cacggtaaac
     5761 tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata acaaataata
     5821 aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc cactgccttt
     5881 tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc tgtgcctgtg
     5941 gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt gaaggagtgg
     6001 taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc gattcaggta
     6061 atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata gactcaattt
     6121 caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca cgaagtctaa
     6181 cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta acatcaacgt
     6241 tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc aaagcctgac
     6301 tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt tggtgattta
     6361 tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg acctccggga
     6421 attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg gttcctggcg
     6481 caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc gccagttggg
     6541 cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt ccggcgtctg
     6601 ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca ccttccagta
     6661 tggcaacctg ttggctgagc gctggttgtg cgatatgcaa tacttcagca gcctgggtca
     6721 ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc atgttgcctc
     6781 cggtttttaa gaatcggccc aagtgccgcc attacttaca accagtatgc aagatgcctg
     6841 ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga gattatgata
     6901 gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa aaggagggta
     6961 tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt tgaaattggc
     7021 agcaaacaaa caaaaaaaat gcatttcacc ctttgacatc accatgcact gccattaata
     7081 tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     7141 gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct gctttcgagc
     7201 aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac cagaactaat
     7261 ttgcacaggc attcccgatc gatgttgcaa cgcagcattt gcgcgattta catcaacttc
     7321 ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt ttcggtcaga
     7381 ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact ttgaaccatc
     7441 cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc ctacaggaac
     7501 attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct gacttacacg
     7561 gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc caatataaat
     7621 cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca aactctcccc
     7681 tcgtttcgca tattcgcgcc ggatgttcgg cgttggcgtc caggttggtg cttcttgttt
     7741 acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc cgataccaat
     7801 aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca tttcagcgac
     7861 gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg gaatggtgag
     7921 ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca gcatattact
     7981 taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt gatcaggaac
     8041 agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg gataagttac
     8101 cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat ttacacgacg
     8161 catcatgtcc ctttcctaag tcgcgaatac tatccgataa gtatagcttt tatcagactt
     8221 ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata ttactggcag
     8281 caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatggac atcgccagag
     8341 ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc agccccaaat
     8401 gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg agaaggaatg agatacggtt
     8461 tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga aatccatcca
     8521 gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt ccgaccaaat
     8581 caaatccacc cacttttgcc aggacatcaa caccatcctg aggatttggt tgattcaacg
     8641 taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc aggtttgcgc
     8701 caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt gccgctgccg
     8761 gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg tttttcgcca
     8821 gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg cgactcattg
     8881 ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata agcccgggga
     8941 taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc gcttgtgctg
     9001 ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg gctgtcactt
     9061 cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac ataaccagta
     9121 ccgctttttt gcccacatgc ggtatgccat tcacccccgg cattcctgcc agttgtatgg
     9181 caagcgcctc cagctttcct aggctaccaa caggtttgaa taacccgtca acatgccgtt
     9241 gtgcacgcga catagcggca gaattgatgg ctgggatcgt attcagtaaa tcggcaatta
     9301 tttgcatgtc acgtttcttt atagcagtgc cagcaggaag accagttcac caagctctat
     9361 ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa gctgaccgag
     9421 gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac ccagcaataa
     9481 tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc catcaatttt
     9541 gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat acatcaataa
     9601 tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga tcggttcgcc
     9661 acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa tcaatgccag
     9721 accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat cgcgactacg
     9781 tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatt ccccggtcat
     9841 cagcgccagc acaagtacgc taaacagtgc cgccagtggt gcgccacacc atgcctgcag
     9901 caccatgaag accagcccgc taatcgcgcc aagcaataat ccaatcaaag gaaaagtaat
     9961 aataccgcga gaataatgtt cgaaatccag tccctgggac cagcgacgcg gtacaggcag
    10021 acgcgtaata aatgagagca tcgcccaaaa taatttgctc atttgatttt tactccaata
    10081 cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt tacccgcccg
    10141 gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat tcccacttcg
    10201 ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa cgactgaatc
    10261 tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggt
//
LOCUS       DAJTYH010000128        10245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTYH010000128 DAJTYH010000000
VERSION     DAJTYH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..182
                     /locus_tag="OO609_003422"
     CDS             <1..182
                     /locus_tag="OO609_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6634714.1"
                     /translation="HLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITFDNV
                     ALQIRTDHAPLDFTK"
     gene            157..387
                     /gene="ymcE"
                     /locus_tag="OO609_003423"
     CDS             157..387
                     /gene="ymcE"
                     /locus_tag="OO609_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6634715.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            377..550
                     /locus_tag="OO609_003424"
     CDS             377..550
                     /locus_tag="OO609_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6634716.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(599..1672)
                     /locus_tag="OO609_003425"
     CDS             complement(599..1672)
                     /locus_tag="OO609_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6634717.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1774..4485)
                     /gene="torS"
                     /locus_tag="OO609_003426"
                     /pseudo
     CDS             complement(1774..4485)
                     /gene="torS"
                     /locus_tag="OO609_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4568..5596
                     /gene="torT"
                     /locus_tag="OO609_003427"
     CDS             4568..5596
                     /gene="torT"
                     /locus_tag="OO609_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6634718.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5569..6261)
                     /gene="torR"
                     /locus_tag="OO609_003428"
     CDS             complement(5569..6261)
                     /gene="torR"
                     /locus_tag="OO609_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6634719.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6348..7520
                     /gene="torC"
                     /locus_tag="OO609_003429"
     CDS             6348..7520
                     /gene="torC"
                     /locus_tag="OO609_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6634720.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7520..10060
                     /gene="torA"
                     /locus_tag="OO609_003430"
                     /pseudo
     CDS             7520..10060
                     /gene="torA"
                     /locus_tag="OO609_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10057..>10245
                     /gene="torD"
                     /locus_tag="OO609_003431"
     CDS             10057..>10245
                     /gene="torD"
                     /locus_tag="OO609_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6634721.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2463 a         2637 c         2738 g         2407 t
ORIGIN      
        1 aacatctcca tttccgctgt ccatgttgtc atggttcaca gtaccgtaca tcggcattcg
       61 atgtgacgga gcgaaaccct ttgggcgcta agtgtatttt ttgtaaatcg actatgatca
      121 cctttgataa cgtcgcgctg caaatacgca ctgaccatgc gccgctggat ttcacaaaat
      181 aatatcaggc tccctcgtgg agcctttttt atatctgcct tatttttctt caacgctgta
      241 tgtatagtaa gcgataacct gttgattatt gaatctttcg ggaagatggc ttataacatt
      301 tcttacctga ccagggtacc gggaaccaac accttactgg cgtgttgctg tctttcaaga
      361 ccagacgagg ttaacagtga atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc
      421 cgactttatc gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag aagtctctga
      481 aattcgcttc atcgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa
      541 aataatgtga ttttgtgaac atcaccccgt gcgaggtgat gttccgcttg ttgctaattt
      601 agtgaccaat cattggcgct tgtggaatta agcgtcggta caattcctcc ggcaccgggc
      661 tttgccatac tcccgcatac attgcgtaac caatcaccgc aaacataatc cccagaacca
      721 gtagcgtcat taaccagcca gacaacgcaa aggctttttt atttgctgca ggtttttgca
      781 gtgaaaaggt caatgttgag gctaccggac atgactctac gcaagtcata cagccggtac
      841 attccactgt tcgtacctga attaatttat ctaccgggat ccgtgatggg cacttttttg
      901 cgcatttgcc acaatcgata caactttcgg cattgcgacg aatcttaaac ggcgacaata
      961 gcgaaaccac gcccatcagc gcgccatatg ggcaaagata acgacaccag gcatggcgaa
     1021 taaacaggct ggcaatcagc aaaacggtca cgctgattaa tgtcgcggtc cccatatgac
     1081 gaaagaaatc gagcatttta acgtccatca ccacgctata gggcgacaac ataaaatagt
     1141 gaatcgcctg agcgggcatc aataacgcga tatagagaaa taaactcaac agcaaatact
     1201 tcacgccgcg cagaggaata tccagccagc ggggaaggac acattgccga ccaaacagtt
     1261 tgttaccgag atcgccgatt aattcagaaa gcgtaccaac cgggcataac catgagcaaa
     1321 aggccttttt gagtaataga ctgatgacga taaaagcgac caataacagc atcgcggcgg
     1381 cgtggacgga cggtaactga cctgttgcaa ggctatattt cagattcatc agaccggcaa
     1441 tcggtagcca gccttcgata cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt
     1501 cgtaatagcg cacccaatac cagaacgtga tggcaatata aatattcatt gccaatagta
     1561 agaattgcgt cgctttacgc caggtcgtgg cattgcgcca gtcattccac ggtaatttgc
     1621 cgcccgtcgt gcctggccgc cgctgccagc gggttctttt tttctctgcc atgattttgc
     1681 cagtccgtta agttgcatac caaatgccac tattctagtt attcttaact ggctgatatt
     1741 gattcaaatc gcgttcaggt ctttcttatt caatcatgct tccagagcgg caacactgcg
     1801 tgtaatttct tcgtgtggaa ggggggcaga taatggctgc tgctccagtt gtgcgcatag
     1861 ctgactggcg ctacgcattc ccagactcga gcaactgctt tttagctgat gcgcggcacg
     1921 ctttattttc tcgctgtcct ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag
     1981 ggcatgttgt ttaaataatg ccagccattc gtggatcttc tccgtcccca ttaactgagc
     2041 gtcttcattg agttgcgaga catccagtgg tagatcgtta ttggcttgca gttggagata
     2101 gtgcgccagt aactgaccga gcacttcacg cggcaccggt ttagggataa tcccgcgaaa
     2161 tagtgaactg gtacgctggc gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa
     2221 aaccagcgac ggatattgct gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg
     2281 cagatcaaaa tccaccagtg cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc
     2341 ctgcgcggca ttgccaatag caacaacctg cgcaccactg gtgttcagca tctcaacggt
     2401 aattcgctgg gttagcgggt tatcttcaat taacagcaaa cgtaaaccgt caagacgcac
     2461 cgcctgattg gctgtttttg gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca
     2521 aaaacagctt ccaacctccg gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc
     2581 cagacggcta ctgatagtca gccccagccc ggtgccgccg cgtttgccgc ttacctgcac
     2641 aaatggctgg aagatttctg ccagtttcgc gggatcaata ccgcagccgc tgtcttccac
     2701 ttcgaccagc cattgctcgc catcagtgcg actacgcagg ataatgtgcc cttcgtcagt
     2761 aaaacgcagg gcattgctca ataggttggt aatgacctga cgaatacgtc gtggatcgcc
     2821 cattaacgcg ctcggtacat catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt
     2881 cacccgtccg ctcattaatt gcagggtact ttccagcagc gggcgcggtt caaagggctc
     2941 atcgctgact gaaacatttt tgccacctgc ttcgatagcc gaataatcga gaatatcgtt
     3001 gaggatggtc agcaacgatt cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc
     3061 gttaagtgcg gggttatctg ccagcagttg agcagtgccg agaataccgt acagcggtgt
     3121 gcggatctca tggctcatcg ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc
     3181 ttccgcccgt gcctgtcggt gttctatcac cagttcctgc aattcagctg tacgcgcttt
     3241 gacctgcgcc gccagctgtt cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc
     3301 atccatcagc cgcccgatgg tatccagctc ccgtacgcca gcggtttccg ggaaagggga
     3361 gtcaatatca ccgtccagca gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt
     3421 gaatgagcga taaaccacgc gccagaggat cataatcagt gcgcaaagtg aaaccatccc
     3481 cagcaataac aggctgtatt gcccgcgtgc actggctttt tccagatgtg ccagtccatg
     3541 ctgattgtgc agctcaatgg tgtcgaccag ctgactgact tcgctactaa actgcgcgaa
     3601 ctgggcgata ttattttggg cgagagtttg caggtgattg ctgatttcac tgtcctgctg
     3661 gaacagcgcc aacagatcgc tatattggct aacggtagtt aacgttgttg cgacctgcgc
     3721 acgaactccc ggatcttcaa tgcgtatttg ccgacgttgc agaattttca ccgcattatt
     3781 gagctgcttt tccagcgttg gtgcattttt ctggatctgc tccagcccca gattcatcac
     3841 catttgctgc acccgcagag cgctaaggcg cagttcattc atctggttaa catactcaag
     3901 atcgatatca atcagccgat cgagtgcact ttcagcagcc tgacgctgat cttgttcgat
     3961 caaatcgtaa atcccggcct gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc
     4021 ttgctccagg cgtgcgatct catcggcggc agctatctgc tggctgagtt gccgttgttg
     4081 ctggcgtagt tgcagacgcc gccccaccag ttccccttgc tgacgtaatg aacgggagat
     4141 ctcctgctcc tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag
     4201 caacgcatta atcttcaggc tttatgcggt gagcattcgc ccctgcgcct gccacatctt
     4261 ttcgttatcg gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct
     4321 caactggcgc gccatattca tggtaggaat caatgcctga gtattgtctt tttccacctg
     4381 gctgataaag cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat
     4441 cagggcaaag cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa
     4501 ggtgaaaaag gttgagtcgc aaagtggaat gcatctagca taaagcctta ttattgatga
     4561 ggctatcatg cgcgtactgc tatttttact tctttccctt ttcatgttgt cggcattttc
     4621 ggctgataac ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag cctctatgcc
     4681 gcttaaagcc aaacgcgtat ggaaactgtg cgcgctttat cccagcctga aagattcata
     4741 ctggttatcg ttgaactatg gtatgcagga ggctgctcgc cgctacggtg tggatttaaa
     4801 agtgctggag gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg
     4861 taaacagtgg ggcgcagagg ccattttgct cggtagtagc acgacgtcat ttcccgacct
     4921 gcaaaagcag gtagcaaatc tgccggtgat cgaactggta aatgctcttg atgatcccca
     4981 ggtgaaaagc cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt
     5041 ggtgcaatgg gcgcacggta aaccactgaa cgtgctgctg atgcccggac ccgataacgc
     5101 cgggggcagt aaggagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg
     5161 tattgttgat attgcgcttg gtgataacga tattgaaatc cagcgcaacc tgttgcagga
     5221 gatgctggaa cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc
     5281 ggcaatgggt gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc
     5341 acatcaggtg tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat
     5401 ggtctggcag ggggaactgg cagttgagca ggccatcagg caattacagg ggcaatcggt
     5461 gtctgataat gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgagaaca
     5521 tatccgccgc tcgctgtcac cggggggatt tcgtccggtc tatttttatc agcacacatc
     5581 agcggctaag aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg
     5641 acgtaaacga cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc
     5701 agaaagcata cgcagcagac gctcacggct gagaatttcg cccggattag tcacaaatgc
     5761 caccaacatt tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt
     5821 atggcgcgac acattcaggc aataacccgc aaagcgatag cagtagtcct gagtgtgcgg
     5881 ttgagcttgt cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc
     5941 gcgcagttcc agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg
     6001 gtcaatccga tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag
     6061 ggcgcgggtt aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc
     6121 taccggctga ttctgcataa tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt
     6181 atacccctcc tgagtgaagt aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac
     6241 aataacaatg tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt
     6301 tgctcattaa gatcgcttca ctaaaccata attctacagg ggttattatg cggaaactct
     6361 ggaacgcgct acgccgaccc agtgctcgtt ggtcggtact ggcgctggtc gcaattggga
     6421 ttgtgattgg cattgcgctg attgtattgc cacacgttgg gatcaaagtc accagcacaa
     6481 ccgaattttg tgtcagttgc cacagtatgc aaccggtgta tgaagaatat aaacagtcgg
     6541 tgcatttcca gaacgcctcc ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg
     6601 atattccagg catggtgaag cgcaaactgg aagcgagcaa cgacatttac cagaccttta
     6661 ttgcccactc cattgataca cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc
     6721 gtgaatgggc gcgaatgaaa gaaaacaact cggcaacctg tcgctcctgc cataactacg
     6781 atgcgatgga tcatgccagg cagcatcctg aagcggcacg tcagatgaag gtggcagcga
     6841 aagataatca atcctgcatc gactgtcata aaggtattgc ccaccagtta ccggatatga
     6901 gtagcggctt ccgtaagcag ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca
     6961 cgctgtactc tattgatatt aagccaattt atgcggcgaa aggcgataaa gaagcctctg
     7021 gttctctgct gcctgcttcg gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa
     7081 ttgaaattac cggctggacg gaaagcgccg gacgtcagcg tgtactcacc caattcccag
     7141 gtaaacgcat ctttgttgcc tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg
     7201 agaaaaccac cgttgccgac accaataccg agtggagcaa gttgcaggcc accgcgtgga
     7261 tgaagaaagg cgacatggtg aacgatatca aaccgatctg ggcctatgcg gattcgttgt
     7321 acaacggcac ctgtaacgag tgccacggcg caccggaaat cgcccacttt gacgctaacg
     7381 gttggatcgg cacgctcaac ggcatgattg gctttaccag tctcgataaa cgtgaagaac
     7441 gcactttgtt gaaatatctg caaatgaatg cgtctgacac cgcaggtaag gctcacggcg
     7501 ataagaagga agaaaaataa tgaacaataa cgatctcttt caggcatcac gtcggcgttt
     7561 tctggcacaa ctcggcggct taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc
     7621 gcgccgtgca actgcggcgc aagcggcgac tgaggctgtc atctcgaaag agggcattct
     7681 taccgggtcg cactgggggg ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc
     7741 aaaaccgttc gaactggata aatatccgtc gaaaatgatc gccggattgc cggatcacgt
     7801 acacaacgcg gcacgtattc gttatccgat ggtacgcgtg gactggctgc gtaagcgcca
     7861 tctgagcgac acctcccagc gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc
     7921 cctcgacatg ttctatgaag aactggaacg cgtacagaaa actcacgggc cgagtgcctt
     7981 gctgaccgcc agtggttggc aatcgacggg gatgttccat aacgcttcgg ggatgctggc
     8041 gaaagctatt gccttgcatg gtaatagcgt tggtacgggc ggagattact ctaccggtgc
     8101 tgcgcaggtg atcctgccgc gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc
     8161 ctggccgctg gtattgcaga acagcaaaac cattgtgctg tggggctccg atttgctgaa
     8221 aaaccagcaa gcgaactggt ggtgcccgga tcacgatgtt tatgaatatt acgagcagct
     8281 aaaagcgaaa gtcgccgccg gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc
     8341 cacccatgag tatctggggc gcgagcatgt gaagcacatt gcggttaacc cgcaaactga
     8401 cgtgccgctg caactggcgc tggcacatac gctgtacagt gaaaacctgt acgacaaaaa
     8461 cttcctcgct aactactgtg tgggttttga gcagttcctg ccgtatctgc tgggtgagaa
     8521 agacggtcag ccgaaagatg ccgcatgggc tgaaaaactg accggcattg atgccgaaac
     8581 cattcgtggg ctggcgcggc agatggcggc gaacagaacg caaattattg ctggctggtg
     8641 cgtgcagcgt atgcagcacg gtgaacagtg ggcgtggatg attgtggttc tggcggcgat
     8701 gctggggcaa attggcctgc caggtggtgg ttttggctgg cactacaacg gcgcaggcac
     8761 gccggggcgt aaaggcgtta ttctgagtgg tttctccggc tctacgtcga ttccgcctgt
     8821 tcacgacaac agtgactaca aaggctacag cagcactatt ccgattgccc gttttatcga
     8881 tgcgatcctc gaaccgggga aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc
     8941 gctgaaaatg tgtatttttg ccggaactaa cccattccat cgccatcagc agatcaaccg
     9001 cattattgaa ggctggcgca agctggaaac ggttatcgcc atagataacc agtggacctc
     9061 aacctgccgc tttgccgata tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct
     9121 cgaccagtac ggcaaccact ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc
     9181 gtagttcgag gcgcgcaacg actttgatat tttccgcgag ctgtgccgtc gctttaatcg
     9241 tgaagaagcc tttaccgaag ggctggacga aatgggctgg ctgaaacgca tctggcagga
     9301 aggtgtacaa caaggcaaag gacgcggcgt tcatctgcca gcgtttgatg acttctggaa
     9361 taacaaagag tacgtcgagt ttgaccatcc gcagatgttt gttcgccacc aggcattccg
     9421 cgaagatccg gatctcgaac cgctgggcac gccgagtggc ctgattgaga tctactcgaa
     9481 aaccatcgcc gatatgaact acgacgattg tcaggggcat ccgatgtggt ttgagaaaat
     9541 cgaacgctcc cacggtgggc ccggctcgca gaagtatccg ttgcatctgc aatctgtgca
     9601 tccggatttc cgacttcact cgcagctatg tgagtcggaa acgctgcgtc agcaatatac
     9661 ggtagcgggt aaagagccag tgttcattaa cccgcaggat gccagcgcgc gcggtattcg
     9721 taacggtgat gtggtacgcg tctttaacgc tcgcggtcag gtgttggcag gagcagtagt
     9781 ttctgaccgc tatgcacccg gcgtggcgcg aattcacgaa ggggcatggt acgatccaga
     9841 taaaggcggc gagcctggtg cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga
     9901 catcgggact tcgcagctgg cgcaggcgac cagtgcgcac actacgctgg tggaaattga
     9961 gaagtacaat ggaacagtgg agcaggtgac ggcgtttaac ggccccgtgg agatggtggc
    10021 gcagtgcgaa tatgttcccg cgtcgcaggt gaaatcatga ccacgctgac agcacaacag
    10081 attgcctgtg tttacgcctg gctggcgcag ttgttctccc gtgaactgga cgatgaacaa
    10141 ctgacgcaaa tcgccagtgc gcagatggct gaatggtttt cgttgctgaa aagcgaaccg
    10201 ccgctcactg cggcggtgaa tgatctggaa aacagtattg ccacg
//
LOCUS       DAJTYH010000129        10136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTYH010000129 DAJTYH010000000
VERSION     DAJTYH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..270)
                     /locus_tag="OO609_003432"
     CDS             complement(<1..270)
                     /locus_tag="OO609_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6634722.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="OO609_003433"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="OO609_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="OO609_003434"
     CDS             complement(1130..1336)
                     /locus_tag="OO609_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6634723.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="OO609_003435"
     CDS             complement(1443..2006)
                     /locus_tag="OO609_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6634724.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="OO609_003436"
     CDS             complement(3381..5216)
                     /locus_tag="OO609_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634725.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="OO609_003437"
     CDS             5317..5604
                     /locus_tag="OO609_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6634726.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="OO609_003438"
     CDS             5576..7105
                     /locus_tag="OO609_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6634727.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="OO609_003439"
     CDS             complement(7275..8492)
                     /locus_tag="OO609_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6634728.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="OO609_003440"
     CDS             complement(8859..9845)
                     /locus_tag="OO609_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634729.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9842..10136)
                     /locus_tag="OO609_003441"
                     /pseudo
     CDS             complement(9842..10136)
                     /locus_tag="OO609_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3183 a         2296 c         1895 g         2762 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga
     9961 aagctaccgg ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta
    10021 tgagttaagt gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct
    10081 gttacgttca tcagaacctg atgctctggc gaagcgatat ccagtccggc aagata
//
LOCUS       DAJTYH010000130        10106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTYH010000130 DAJTYH010000000
VERSION     DAJTYH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            134..265
                     /locus_tag="OO609_003442"
     CDS             134..265
                     /locus_tag="OO609_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6634730.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            525..1520
                     /gene="pgl"
                     /locus_tag="OO609_003443"
     CDS             525..1520
                     /gene="pgl"
                     /locus_tag="OO609_003443"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6634731.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1561..2514)
                     /locus_tag="OO609_003444"
                     /pseudo
     CDS             complement(1561..2514)
                     /locus_tag="OO609_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2698..3750
                     /locus_tag="OO609_003445"
     CDS             2698..3750
                     /locus_tag="OO609_003445"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6634732.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3826..5259
                     /locus_tag="OO609_003446"
     CDS             3826..5259
                     /locus_tag="OO609_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6634733.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5442..7622
                     /locus_tag="OO609_003447"
     CDS             5442..7622
                     /locus_tag="OO609_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6634734.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7763..9046)
                     /locus_tag="OO609_003448"
     CDS             complement(7763..9046)
                     /locus_tag="OO609_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6634735.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9181..10062)
                     /locus_tag="OO609_003449"
     CDS             complement(9181..10062)
                     /locus_tag="OO609_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6634736.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2453 a         2359 c         2552 g         2742 t
ORIGIN      
        1 tcaatgtact gtttttttct ttaaaaaaac agtacattct attttataat gactgtgtat
       61 tcccatggaa ggagatcacc caattctccc ccccgacttt tgacgcagtg tcttgttaac
      121 agggggattg accatgctta ttggctatgt acgcgtatca acaaatgacc agaataccga
      181 tctacaacgt aatgcgttta tttgtgccgg aagtgagttg attgttgcgc gaatgggaag
      241 ttggccagaa gttgttatgg catagtgttt acggtcgttg atacaggtaa acaaaagcgg
      301 ggagagtcat actccctgaa tagtcgcgaa aatatctgtg gcattgtcaa attcgtgtcg
      361 gtgactaata ttttagtaat atgccattcg aaatggagtg ctaacttatt gatttaattg
      421 atgtgcgata ttgtatttta tcgtgttttg ttgtctgttt tttatccagt aagtgttgtt
      481 atatgtatga aagataaggg atttatcatt tttgaggtgt gttcatgaag caaacagttt
      541 atatcgccag ccctgagagc cagcaaattc acgtctggaa cctgaatcat gaaggcgcac
      601 tgacgctgac acaggttgtc gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg
      661 acaaacgtta tctctatgtt ggtgttcgcc ctgagtttcg cgtcctggcg tatcgtattg
      721 ccccggacga tggcgcactg acctttgccg cagagtctgt gctgccgggt agtccgacgc
      781 atatttccac cgatcaccag gggcagtttg tctttgtagg ttcttacaat gcgggtaacg
      841 tgagcgtaac gcgtctggaa gatggcctgc cagtgggcgt cgtcgatgta gtcgaggggc
      901 tggacggttg ccattccgcc aatatctcac cggacaaccg tacgctgtgg gttccggcat
      961 taaagcagga tcgcatttgc ctgtttacgg tcagcgatga tggtcatctg gtggcgcagg
     1021 accctgcgga agtgactacc gttgaagggg ccggcccgcg tcatatggta ttccatccaa
     1081 acgaacaata tgcgtattgc gtcaatgagt taaacagctc agtggatgtc tgggaactga
     1141 aagatccgca cggcaatatc gaatgtgtcc agacgctgga tatgatgccg gaaaacttct
     1201 ccgacacccg ttgggcggct gatattcata tcaccccgga tggtcgccat ttatacgcct
     1261 gcgaccgtac cgccagtctg attaccgttt tcagcgtttc ggaagatggc agcgtgttga
     1321 gtaaagaagg cttccagcca acggaaaccc agccgcgcgg cttcaatgtt gattacagcg
     1381 gcaagtatct gattgccgct gggcaaaaat ctcaccacat ctcggtatac gaaattgttg
     1441 gcgagcaggg gctactgcat gaaaaaggcc gctatgcggt cgggcaggga ccaatgtggg
     1501 tggtggttaa cgcacactaa ccgctgattt aaccggcgca gtctctcctg cgccggtgta
     1561 ttaacttatc tcctgtaacg catgtctctg gcgttcgacg atattggtcc acaaattgtc
     1621 tttatcgtca gtccacaaat taatcagcaa ggaaaaaaag cgttcagctg ccggagaaag
     1681 cacggcatct ttgcggcgaa taatccccaa tgtccgacgt ataacgggtt ccaccagtgg
     1741 gataccaata atcgaagaat agggcgcatg gggcattgcc agaccaggaa gcgccgaaat
     1801 ccccagtcct gcctccacta gtcctaatga cgtcgaaaga tgacgcactt cgtaaaacca
     1861 gtccagcttc cagggcttgt cggctagctg ttgttctatc aacaatcggt tgccgctgga
     1921 ggaacgtacg ccaatcattt tgtaacccac taactcctgc cattctacaa gctacttttt
     1981 ggccaatgga tggtcacgcc gacaggccag cacgaatggc tcgttgacca gtggggtaaa
     2041 atcaatggat gaatttgtga cgttgttcat gtttatgcca aagtctgatt cgttacacag
     2101 caccgattcc atgcaattat tcgtgccttg ttccagaatc cgcactttaa tattgggata
     2161 cagctcatta aatttaccga ttgccagcgg taaaaaataa aatactgcgg tcggaataca
     2221 cgctaatgtc accataccac gatgataagc attcatatca cgaatattaa aaagcgtttc
     2281 atcgaatttc tttattaatt cccgcgcctc cgggagcaac ctttttcctg cttttgttaa
     2341 cgtaaccttg cgagttgtac gctcaaatag ttgaatatgt aaatcttcct ccatcttttt
     2401 tattcttcgc gttaatgcag gttgcgtaat attgagtaat ttagcggcat tgttaaatga
     2461 actggactct gccagtatga caaatgcctt catacttgat aattcatgct tcattattac
     2521 tccggaaaat ggaagcgacg attttggggg gctggtcgtt aaaaatttta actgcattta
     2581 gccaacttaa attaatgaaa aaatgttatt aatcgttgag ctaaagtcat tagagatgct
     2641 ttgtccttaa tgtaaccata tcgcaataag ttatgttttt aaattgaggg cattattatg
     2701 aaaaaaatac cctgcgtgat gatgcgaggt ggaacctcga ggggcgcgtt cctgttagcg
     2761 gaacatttac ccgaagatca aacgcagcgc gataaaatat tgatggcaat tatgggttcc
     2821 ggtaacgatc tggaaattga cggtattggc ggcggtaatc cactgaccag taaagtcgcc
     2881 attattagcc gttccagcga tccgcgtgct gatgtcgatt atctgtttgc tcaagttatc
     2941 gtccatgagc aacgtgtcga taccacgcct aactgcggca atatgctgtc tggcgtaggg
     3001 gcattcgcca ttgaaaatgg tttgattgca gcgacttcgc cagttacccg tgtacgtatc
     3061 cgcaacgtca atacgggtac gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc
     3121 gagtacgagg gtagcgccag aattgacggc gtaccgggta ctgccgcacc ggttgcgctc
     3181 actttcctga atgccgctgg aacgaaaact ggaaaagttt tcccgactga taatcagatt
     3241 gattattttg acgatgtccc ggtgacctgt atcgatatgg cgatgccagt cgtcattatt
     3301 ccggctgaat atctgggaaa aacaggttat gaattaccgg cggaactgga tgccgacaaa
     3361 gcattattag cccgcattga atctatccgg ctacaagcgg gtaaagcaat gggcttaggt
     3421 gatgtcagta atatggttat ccctaaacct gtgcttattt ctccagcgca gaaaggcggg
     3481 gcaattaatg tgcgttattt tatgccgcat tcttgccatc gcgcgctggc gataaccggt
     3541 gctattgcta tttccagtag ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct
     3601 tctgtaggat atggcaatat caatattgaa caccccagtg gtgcgctcga cgttcattta
     3661 agtaatgaag gtcaggatgc cacgacgtta cgcgcatctg ttattcggac aaccagaaaa
     3721 atattttccg gtgaagttta tcttccctga aaaaattcgt tgtcaggata aggacaatca
     3781 ataaaggact tctgtatgag tcatacagaa agaacaggat tttaaatgaa taagaaatcg
     3841 ttatggaagc taattctgat attagcgatc ccatgtatta ttggttttat gccagctccg
     3901 gcaggattaa gcgaactggc gtgggtgctt tttggtattt acctggcggc cattgtgggt
     3961 ctggttatca agcctttccc ggaacctgtc gtactgttaa ttgccgttgc tgcctcaatg
     4021 gtggtggtcg gtaacttatc cgacggtgcg tttaagacca ccgccgtatt aagcggttac
     4081 tcttcaggta ctacctggct ggtgttctcg gcgtttacct taagcgccgc atttgtgacc
     4141 accggtttag gtaaacttat tgcctatctg ctgattggta aaatcggtaa caccatgctg
     4201 ggtctgggtt acgttacggt attcctcgat ctggtactgg ctccggcaac accgtctaac
     4261 accgcgcgtg cgggcggcat tgtgttaccg atcatcaaca gcgtggcggt ggctttgggg
     4321 tccgaaccgg aaaaaagtcc gcgtcgtgtc ggacattacc tgatgatgtc catttacatg
     4381 gtcaccaaaa ccaccagcta tatgttcttt accgcaatgg cggggaacat tctggcgctg
     4441 aaaatgatca acgacattct gcacctgcaa attagctggg gtggatgggc gctggccgcc
     4501 ggattgccgg gcatcattat gctgctggtc accccgctgg taatttacac catgtatcca
     4561 ccagaaatta aaaaggtgga taacaaaacc atcgctaaag cgggccttgc cgaactggga
     4621 ccgatgaaaa tccgcgaaaa aatgctgctc ggtgtctttg tgctggcgct actgggctgg
     4681 attttcagta agtctctggg ggttgatgaa tccactgtgg caatcgttgt tatggcgacc
     4741 atgctgctgc tgggtatcgt tacctgggaa gacgtggtta aaaataaagg cggctggaat
     4801 accttaatct ggtacggcgg tattatcggc ttaagctcct tattatcgaa agttaaattt
     4861 ttcgaatggt tagctgaagt ctttaaaaat aacctggcat ttgatggtca cggtaacgtt
     4921 gctttcttcg ttattatttt cctcagcatc atcgtgcgtt atttcttcgc ttccggtagt
     4981 gcctatatcg ttgctatgtt acctgtattt gccatgctgg cgaacgtctc cggcgcaccg
     5041 ttaatgttaa ccgcgctggc actgttgttc tccaactcct atggcggcat ggttactcac
     5101 tatggcggcg cggcaggtcc ggtcatcttt ggcgtgggtt acaacgatat taaatcctgg
     5161 tggttggtcg gtgcggtact gacgatatta accttcttgg tgcatatcac cctcggcgtg
     5221 tggtggtgga atatgctgat cggctggaac atgctgtaaa tatactcgtc atacttcaag
     5281 ttgcatgtgc tgcgtctgcg ttcgctcacc ccagtcactt acttatgtaa gctcctgggg
     5341 attcactcac ttgtcgcctt cctgcaactc gaattattta gagtatatcc atttattatc
     5401 tttctgcgca cttcacggtg cgcagatatc tggagcattt gatgatcaag ttatctgaaa
     5461 aaggcgtgtt tctcgccagt aataacgaaa taattgccga agaacatttc accggcgaaa
     5521 ttaaaaaaga agaagcaaaa aaaggcacta ttgcctggtc tattctctct tctcataata
     5581 cgtccggaaa tatggataaa cttaaaatta agtttgattc attagcctct cacgatatta
     5641 cctttgttgg tattgtacag accgctaaag cgtccggaat ggaacgtttc ccgctgccgt
     5701 atgtgctgac caactgccat aactcactct gcgccgtcgg cggcaccatt aacggtgatg
     5761 accatgtttt tggtttatcg gcagctcagc gttatggcgg tatttttgtg cctccgcata
     5821 ttgcggtcat ccatcaatat atgcgtgaga tgatggctgg cgggggcaaa atgatcctcg
     5881 ggtcagacag ccacacccgt tacggtgcat tagggacaat ggcagtcggt gagggtggcg
     5941 gtgagttggt aaaacagctg cttaatgaca cctgggatat cgactatccg ggcgtggttg
     6001 cggtgcatct gaccggaaaa cctgcgccgt atgtggggcc acaggatgtg gcgctggcta
     6061 tcattggcgc ggtgttcaaa aacggttacg tcaaaaacaa agtcatggag ttcgttggac
     6121 cgggcgttag cgcgctctct accgatttcc gtaacagcgt tgacgtgatg accacggaaa
     6181 ccacctgttt aagctccgtc tggcaaactg atgaagaagt gcataactgg ctggcgctgc
     6241 acggtcgcgg tcaggattac tgccagctta accctcaacc gatggcgtac tacgatggct
     6301 gcatcagcgt tgatttaagc gccatcaaac caatgattgc gctgccgttc cacccgagca
     6361 acgtgtatga aatcgacaca ctgaaccaga acctgaccga cattctgcgt gagattgaaa
     6421 ttgagtccga acgcgtggcg cacggtaaag ccaaactctc gctgctggat aaagtggaaa
     6481 atggtcgcct gaaagtgcag caggggatta tcgcgggctg ttctggcggt aactacgaaa
     6541 acgtcatcgc ggcggcgaat gcactgcgcg gtcaatcctg tggcaatgac accttctcgc
     6601 tggcggttta cccgtcatca cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag
     6661 cagatttgat tggcgcaggc gcaatcatca gaaccgcatt ctgtggccca tgctttggcg
     6721 cgggcgatac gccaatcaac aacggtttga gtattcgcca caccacgcgt aacttcccga
     6781 accgcgaagg ctctaagcca gccaatgggc agatgtcagc ggtggcgttg atggacgctc
     6841 gttctatcgc tgcgactgcg gcaaacggtg gctatttaac ctctgccagc gaacttgatt
     6901 gctgggacaa cgtgccggag tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc
     6961 agggctttgt gaaaggggca actcagcaac cgctgattta cggaccgaac attaaagact
     7021 ggccggaatt gggtgcgctg actgacaata tcgtcctgaa agtgtgctcg aagatcctcg
     7081 acgaagtcac caccaccgac gaactgattc cttccggtga aacctcttct tatcgttcaa
     7141 atccgattgg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     7201 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     7261 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     7321 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     7381 actgggggat gttaccgctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     7441 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     7501 tgatccatga agatgcgccg gtaacggaaa ttacgcttta catggaaagc ctgacggctg
     7561 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     7621 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgctggcg
     7681 catcttatcc gacctacaaa tcgcatcgaa ccgtaggccg gataaggcgt ttacgccgca
     7741 tccggcaaat agttaatagc tcttacttct tctcctctgc aaccactttg ctgcccacgc
     7801 cgcggttatt gtattcccac atgcggttgt agttagtgtc attcagattg cgctgtattt
     7861 cgtcgttatc atctacgctg ccggtattac ccgcaaacgg acgattagag atcaccgcat
     7921 cggcccacgg tttagccgtg ttaaaacctt cgttgatggc gctatcacgg atcaccacct
     7981 gaccgttggt attagcatca acatccagcg agcggcccag ttgcgccacg ccatcaccgg
     8041 aagcattgaa acggctgttt acggcgagga aaccgtaata gatgttagac agcgtagccg
     8101 gtgcaaacac atatgcttct tgctgggtac gtgagttcac cacgcggaat tcggtgttat
     8161 cgaacaccac tgcgccgcga ccagaaacga tatccacatc cccttcaata tagctgttgg
     8221 tcaccagcgt acgcggctga cggttggttt ccagacggtt ctgcacaccg ctgttggtga
     8281 caaagaaggt gttctgacga ccgagaatgt taacgttgtt aatctgtact tggtcaccgt
     8341 cagtacgcag tgctaccgcc ggatggttac ctgcatctac gctatcgccc agcgtgtttt
     8401 cgatggtcag attttgcagt tgcagtccat tgttttgtga ccagaagacc gcagaacaca
     8461 gaacaccgat actgtcgctg cgtttgctct ggcagctatc gtacatatac cacgctggtt
     8521 tacctggcat atatttgccg cgcgggttga cgtcgtgacg ccagtcggca gggctcatgc
     8581 ccccatcaag ggaaagccca atcttcacat caatcggttt ttcacctgta ccgtacagag
     8641 tgattccacc tggagcagca gggacataca ccgttccctg atactcacca ggcatcacgg
     8701 caatatactg gcgcttgttg gtgcgcttga taattgccgc atctaccgcc gcctgaatcg
     8761 tggtatgcgt tacaccttga gtacccgccg ggccgacaac aaagtcaggt tgcgcaggca
     8821 gggttatcgg ggacggattc cacgctgcag cacctggtgt cagggatgca aaatagtgtt
     8881 gcgcatcgaa attcagcgct tcttttgccg acagaatcgg gcgagaagag gtaccaggcg
     8941 cagtttgatc agaaggacgt tgatcgggtg gtgttgagct acaggcggtc agcgtcacgc
     9001 caaaagccaa tgccagcgcc agacgggaaa ctgaaaatgt gttcacaggt tgctccgggc
     9061 tatgaaatag aaaaatgaat ccgttgaagc ctgctttttt atactaagtt ggcattataa
     9121 aaaagcattg cttatcaatt tgttgcaacg aacaggtcat tatcagtcaa aataaaatca
     9181 ttatttgatt tcaattttgt cccactccct gcctctgtca tcacgatact gtgatgccat
     9241 ggtgtccgac ttgtgcccga gaagatgttg agcaaactta tcgcttatct gcttctcata
     9301 gagtcttgca gacaaactgc gcaactcgtg aaaggtaggt ggatcccctt cgaaggaaag
     9361 acctgatgct tttcgtgcgc gcataaaata ccttgatact gtgccggatg aaagcggttc
     9421 acgacgagta gatgcaatta tggtttctcc gccaagaatc tctttgcatt tatcaagtgt
     9481 ttccttcatt gatatcccga gagcatcaac atgcaatgtt gtagggatgg caatttttac
     9541 gcctgttttg ctttgctcga cataaagata tccatctacg atatcagacc acttcatttc
     9601 gcataaatca ccaactcgtt gcccggtaac aacagccagt tccattgcaa gtctgagcca
     9661 acatggtgat gattctgctg cttgataaat tttcaggtat tcgtcagccg taagtcttga
     9721 tctccttacc tctgattttg ctgcgcgagt ggcagcgacc gggtttgttg ttatatggcc
     9781 ttcagctatt gcctctcgga atgcatcgct cagtgttgat ctgattaact tggctgatgc
     9841 cgccttgccc tcgtctatgt atccattgag cattgccgca atttcttttg tggtgatgtc
     9901 ttcaagtgga gcatcaggca gccccctcct tattgcttta attttgctca tgtaatttat
     9961 gagtgtcttc tgcttgattc ctctgctggc gaggattttt tcgtagcgat caagccatga
    10021 atgtaacgta acagaattat cactgttgat tctcgctgtc agaggcttgt gtttgtgtcc
    10081 tgaaaataac tcaatgttgg cctgta
//
LOCUS       DAJTYH010000131         9762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTYH010000131 DAJTYH010000000
VERSION     DAJTYH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..616)
                     /locus_tag="OO609_003450"
     CDS             complement(<1..616)
                     /locus_tag="OO609_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6634737.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMAARI"
     gene            complement(630..1289)
                     /gene="pgmB"
                     /locus_tag="OO609_003451"
     CDS             complement(630..1289)
                     /gene="pgmB"
                     /locus_tag="OO609_003451"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6634738.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1286..3553)
                     /gene="ycjT"
                     /locus_tag="OO609_003452"
     CDS             complement(1286..3553)
                     /gene="ycjT"
                     /locus_tag="OO609_003452"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6634739.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3550..4173)
                     /locus_tag="OO609_003453"
     CDS             complement(3550..4173)
                     /locus_tag="OO609_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6634740.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(4170..4592)
                     /locus_tag="OO609_003454"
     CDS             complement(4170..4592)
                     /locus_tag="OO609_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6634741.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(4614..5389)
                     /locus_tag="OO609_003455"
                     /pseudo
     CDS             complement(4614..5389)
                     /locus_tag="OO609_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(5407..6459)
                     /locus_tag="OO609_003456"
     CDS             complement(5407..6459)
                     /locus_tag="OO609_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6634742.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6490..7332)
                     /locus_tag="OO609_003457"
     CDS             complement(6490..7332)
                     /locus_tag="OO609_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6634743.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(7319..8200)
                     /locus_tag="OO609_003458"
     CDS             complement(7319..8200)
                     /locus_tag="OO609_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6634744.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8221..9504)
                     /locus_tag="OO609_003459"
                     /pseudo
     CDS             complement(8221..9504)
                     /locus_tag="OO609_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(9518..>9762)
                     /locus_tag="OO609_003460"
     CDS             complement(9518..>9762)
                     /locus_tag="OO609_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR6634745.1"
                     /translation="RRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKN
                     IQHVNITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2292 a         2743 c         2462 g         2265 t
ORIGIN      
        1 caatccgcgc cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg
       61 tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc agcttggcat
      121 caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc
      181 cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact
      241 cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc tctttggcga
      301 ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg
      361 cgtagttctg gaacaccatc gcgatattgc gtgctttggc tggaacgtca ttcattcgtt
      421 tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc atgcgcaggg
      481 tggtcgattt accgcagccc gacgggccga caaatacgat gaactcttta tcggcaattt
      541 ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga atatgttgta
      601 acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc cgataaccgc
      661 gaccaggtca gggagtccgt tgagggcaac agtaattgcg ccccggttaa gcccgcaccg
      721 atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca
      781 atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag aaagatttcc
      841 ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc
      901 agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cggaagccag ccctaccgga
      961 atctgctgtg cacggagctc tgccagcaaa ttgcgaatac cgggcagcac agcattaacc
     1021 gtcagctcgc gcagtgagtg gacgtaaagc agatttttgc gatacgccag ttgcgcccgc
     1081 tcctgcgggt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg cagagactca
     1141 tcgcggctga tcccttttag agattcgtta aactgcgcat caatactgat gccaatttca
     1201 gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc
     1261 agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgcgtggta gcggtcccat
     1321 tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg gatataagct
     1381 gaccgtccaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc tgcgcgtcga
     1441 gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt tgccactgct
     1501 caagtaacgc cggatttaga tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct
     1561 gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca tcacaactat
     1621 gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa ctttgggtca
     1681 gcaggccaca gcgtgcagca acaatgccgt ggattgcttt acttaatgac gagtcgtgaa
     1741 tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgatgag aactgctctg
     1801 gcagcatgta attgagcatc accacatcag cttgtttgag gatctgcatc tcgttcactt
     1861 ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg tatttcgcca
     1921 gattaatcgc cggcttagtc ataaacgaat catcctgcgg caaaacaccg tcgggctgaa
     1981 tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac gcgtcgtcac
     2041 tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac accatatagc
     2101 tggtgaaggc gttattattg acatgttcgg tatattcgtc tggcccaata acatcatgaa
     2161 tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca gtttccagaa
     2221 gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa
     2281 taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag gccacttttt
     2341 gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct tcgccgctgc
     2401 gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg
     2461 cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa
     2521 acagatggaa cggcagcaaa aatacttctg tatcccagaa aacgtggcct ttgtagcctt
     2581 cgccggttaa gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc gtcatgatgc
     2641 gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg ccatttaccg
     2701 tgatacggcg tttctgccac cattgacgcc agttttctgt tgatgctgca agatgttggt
     2761 cgtaactctg ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc cactcgtcta
     2821 aagcagcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac gtcactgtct
     2881 cgcctgcatg aagctgcgta ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc
     2941 attgctgcac atcaccgctc acctggcagc aacaactgat ggccacatca ctgcgcccat
     3001 cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt
     3061 cgagatgctg gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa atcagcactg
     3121 acgcgtcggc gtccagtggc ataatagtga tttccagcgc aatgagcggc agttggtctg
     3181 ccgaaacaaa gcgacggctg gcgatggtgt aacctgcgcc gttgctggta cgccagacaa
     3241 cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct tcgcgggata
     3301 acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc agattcacca
     3361 gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac atccctcgcg
     3421 tctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg ttcccctgcg
     3481 ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct gataacgtta
     3541 ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgatata acgccgccac
     3601 cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct tgccctgtac
     3661 gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca tttcccgttg
     3721 catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc
     3781 accggcttta tcaccacaaa agctgacgtt cataatcaac tgttcgcgga tgttgagtgc
     3841 aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc caaacagcga
     3901 atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccacagc tcttttgcgt
     3961 gccgatcttt tgaaaactat gcgcattcac gcttttcacc gccggaaagc ccagcacata
     4021 catcgcagca tccagcatat gaatgccgat gtcgatcagc aggccaccac cctgcagttc
     4081 tttattggta aagacacccc agccgggaac gccgcagcga cgcagggcgc gggcggtggt
     4141 aacgtaaatc tctcccaaaa cgccgttggt cacctgttca cgcagctgtt gcgtatcgag
     4201 cgcaaacgat ggtgaaagtc gtaggccagc accttgccca gtttgcgcgc ggtatcgcac
     4261 atttcccacg cctgttctgt cgtcatggcg ggcggttttt cgcacatcac atggcagcct
     4321 gcttccagtg ccatcagggt atgttcgtaa tgaaaacggt taggtgagcc gacgctaacc
     4381 acatcaggtt tcaccgccag cagcatggcc tgcggatcgt cccacacgga tgcattcccg
     4441 tatttttctg ccagcgcctg cgcctgggaa aggtggctgt cacagacagc caccagttcc
     4501 agatcgttgc gggtgcagta gtacgaagca tgaaccttat ccgccacctg gcccgcgcct
     4561 attatcgcga cccgcagcgg agagcttgtc attgcacttt tcacaaagac ctcttagcag
     4621 gtacgcaacc aggccaacga atcacggtac gcctgggcag gatcttctgc ccggatacgc
     4681 ccttcataca ccacataacc ctgatagtta tcggcgcgca gctgttcaaa cagcgcgtgg
     4741 aaatcgaggg tgccactgcc cggctggtaa cgatggttat cggcaatatg cacatgaccg
     4801 agcaagtcac ggttgtcgtg cagcgcctgc gccaggttat cttcttcgat gttcatgtga
     4861 tagaaatcgc cgataatctg cacatgttta agattgtttt cgacgatgta acgacgagca
     4921 tcggcgaggg tgttgatcat atgatcctga tagcggttta acggttcgag atacaccacg
     4981 gttccggtac gcgcggcgac ctgttccagt acgcgcaggg aatcactcac cagtttgcgg
     5041 tcgccgtcca ggctacgcgg cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac
     5101 gctgccggaa cccccccact tctgccagcg cctcgagaat gcgttcgatc tgctttaagc
     5161 cattaagacg acgctcttcg ataaagtcac caatccagcc gtcatagccg ccgcaggcgg
     5221 tggtcactgg taaaccggtt tctttgatcg ccgctttgac ttcttcgatg ttgttgacca
     5281 gcagtttgcc gtcaatctca aaaccatcga agcccatctc tttgatataa cgaaatctct
     5341 cgcggatatt ttccggaaag aatgcctgat tctgtgtgcc gattttcatt ttttcattcc
     5401 tttgaattaa aacgtaacgc ccattttgat gctctgttcc ggatgctgat cgacatactg
     5461 catgtagctt tccgggctgt tggcaaaggt cactaccggg tcgattaaat cttcgcaatt
     5521 gagataaccg ttcatcagca gttcccagca ggtttcttca atacgcttac ggctccagcg
     5581 cggataatcc gggttcggtt cgctgcacgc gcgggagaaa acaattttgg cgttattgaa
     5641 atgcgcttcg cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg cgacatagga
     5701 gatggtgccg ccgtaggcca ggccgcgcag cgccgattgc agcgcgtcgg cgtaaccgct
     5761 ggtttcgata atcacatcag ccccctgctt gccggtcagc gttttgatct ctttgcctac
     5821 gtcagtgcca atggggttaa ggcagaaatc agcaccgtgg cgacgggcaa tatcacagcg
     5881 atgggcgata ggatcgacgc caattaccac cgatgcgcca gcgcgtttcg ccagttggat
     5941 ggcaatttga ccgatcgcgc caagccctac caccacgaca aaatccccta cgcgtacgtt
     6001 ggcatcgcgc acgccgctca tggcaaactg cgccgggtcg taacagacgg cgtttttcca
     6061 ggagctgcct tgcggcattt tgcgcagctt gtagttattc actgcgttaa tgatgaccgt
     6121 ctcggagagc gggccgtagc cgcatacgct gtcgcccacc gcgtagtctg taacgtcgct
     6181 gccgcactcg ataatgtcgc caaccaccat gttgccaagc tggaatttgc caaactcaat
     6241 gccgcgcggc gcatctgccg gacgcggggt gaacatctgc cactcgccgt taaagtcttc
     6301 atcgataaac gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt tcggtgcgcc
     6361 gaaacgcacg cggatcttca cttcattggc taaaatcgcc cgatcttcat actcaaccag
     6421 cgcagcaaca cgcggtgctg tggctactaa ctttttcatg taaaactcct tgtaaagaaa
     6481 ctggccgctt tagcccttca cgccaccggc ggtcaaacca ctcttaataa aacgttccaa
     6541 aagcgcatac ataatcacca ccggcaatgc ggtcaccagc gaggccgcca tcatccgtcc
     6601 ccagatgtaa tcaggcgtac tgaacagcgc gttcaggccc actggtaagg tgaaattgct
     6661 ggcgctggag aggaaaatcg acgcaaacag atagtcgttc cacgccacca taaagcagta
     6721 gacgaacacc gatatcagcc cggacatcgc caggggcacg gtgatacgga agatgatttg
     6781 cagacggttg aggccatcca tcatcgccgc ttcttcgatc tcatcaggga tggtgtcgaa
     6841 gtaacttttc agcataaaca cggcggtggg cagggtttgc gtcaccatgg taatgatcag
     6901 cgccatctcg gtgtcgtaaa taccaagcgc ggtgatgatt ttgaacagag gcaccaccag
     6961 caaaatgcca gagaacatat acaccgtgta aaagctggcg ttgatggtca tccggccttt
     7021 aaagcgcagg cgggaaagcg cgtaagctcc gagaatgccg agaaataccg ccaccacgga
     7081 ggaaaccacc gacaccacca tgctgttacg gaagtagtcg acaaacggga aaatcatcgg
     7141 gttaaaaatg tcgacgtaat gctccagcgt ccactgttgc ggcagcagcg taggatgcag
     7201 cgatatcgcc tctttcgcgc ttttgaacga ggtcatcagc atcacaaaaa acggaaacag
     7261 cgtgatgatg agaaacagcg ccagcccgca gtaaaaaccg atacgactga gcgtgcgttt
     7321 atttgttgcc attgaggttc acccgtttcc tggtcagcag aatgacagcg aaaataatga
     7381 tgaagagcac caccgagatc gccgccgctt tccctaagtc attaaaggca aaggcggttt
     7441 tgtagagata gacaccaaga atatcgacct tggttgtcag cagataaaca tcagcgaaca
     7501 tgtagaacat ccagatggtg cgcagggtca ccaccgtcgc caggaccggc ataattgcgg
     7561 gcagcgtgac gatgcgaaac cgctgccagg cattagcgcc atccatctct gccgcttcgt
     7621 acagcgattt gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca tacgggaagt
     7681 agcgccagat ggcgaacagc accaccagca caaagctact gcccggatta tcaaaccaca
     7741 gcggtgcctg ctcatagaga tgcagaagat cgacgccgag gtagttcaca atgccgtagc
     7801 cgttgttgaa catgtatttc caggcaaaca ccagcgaaat ggacggcgtt acgtaggaga
     7861 ggatcaccag cgaacgcgcg gttttgcgca ggcggaactc gcggttgaaa aacatcgcca
     7921 cggccagccc gagcacggtg ctgcccgcca ccaccagcgc cgtataccag accgtcatcc
     7981 acagcgagtg ccagaagccg ggatcggaga gaatgcgcac atagttactc accccaacaa
     8041 acgttgactc gatgttggga ttgagcggca gacgtaaaaa actgatttcg atattcgaca
     8101 ccatcggcca cgccaccaga ccgcccagca gtaataagct gggcgcgaga agcagcagcg
     8161 caaacggcat atcggaacga cctgaaaaaa gcctgttcat tgcggattcc tgtcctgggt
     8221 ttagtgctgt tcgatcagtt catccagctt tttctggctc gcttgcagcg tgccagggag
     8281 atcggcttta cccacggtga cgttatgcac catggagctc accacaccag aacccgtcac
     8341 atcgcccatg cgggtaaagt ttttatcccc tactgcgcca aagacctgaa tatttggcag
     8401 ttcaccgatt agctgattcg gtagttcacc cagcgcctta ataacgtcgt tgtctttcca
     8461 ggtggcggta gtgacaaccg ctttattcac cggcagcgca gcacctggcg acatcatcac
     8521 ccagtcggca atgttgtccg cctgctccat aaaggtgaca aatttttctg ctgcttcggt
     8581 cttttgtccg gcggtaatgg tcagcgaggt caacatgccg tagaccgcag agtttttctc
     8641 ggttggcacc acgaaaccga cgtttttcgg gtcgccttct ttaatcacag ccggaaggat
     8701 ataggtggag taaatcgcca tcggcgcggt gccgttcata aaggcgtctt tcacttccat
     8761 gatgtcgtta gaacccggca tggtgttggc agcaaggtcg cggtaatagg tcagcgcctg
     8821 catcatctct agtgtatcaa gggtgatttt gccttcggcg ttaaagacgt tggcctagtt
     8881 ggataacgca aactgggaga aggattgttc cgtcagcacg ctttctgccg taggcagcgc
     8941 aataccgtat tttttattcg ccgggtcatt cagtttctgt gcaacgtcca gcagctgttg
     9001 ccagtttttc ggctcctcca gccccgcttt tgccagcaca tctttgcgat accagatgcc
     9061 gccaatccag gcgctgacag gaacaccggt ccatgcgcta ccatcttcgg tacgcacaat
     9121 acgcagtacg ccatcgtaaa acgcgccttc accaacgttg ctgatgaccg tggcaaccgc
     9181 tttgcgatcg ataagctgct ctttatccat aactttggcg tagtcatggc tggtttcgat
     9241 cacttccggc agcgagccgc tacgtgaaag ggtgatgact ttggtgttat gagcgtcttc
     9301 ttccacgggc acttgtttaa cggtgatacc tgggttttct ttttcaaaac gggcaatcaa
     9361 tttgctgata actgcctgac gctcctgctc caccgaagaa tgcataaatt cgatggatac
     9421 atgggtttta ttttcttctt tacagcctga aatcagggcg caggaaacca gtgctgataa
     9481 cagcacgatt tttgatttaa tcatgagatg ttccttttta tttaatccac ataacctgcc
     9541 aggggcgcag cgttatttca ttacccaata tatcaacttc actaatcaga tcccgaccat
     9601 gcagatcagt aatatttaca tgctgaatat ttttactgac attaaacaat ccagtcagac
     9661 aattaccatc agcgttactt ctttgaatac gcattacgga tgaattaacc gtatcgatgg
     9721 taaaattatt atccggatga aactcgttat ggctgcgacg aa
//
LOCUS       DAJTYH010000132         9757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTYH010000132 DAJTYH010000000
VERSION     DAJTYH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(168..488)
                     /gene="psiF"
                     /locus_tag="OO609_003461"
     CDS             complement(168..488)
                     /gene="psiF"
                     /locus_tag="OO609_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6634746.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(607..2022)
                     /gene="phoA"
                     /locus_tag="OO609_003462"
     CDS             complement(607..2022)
                     /gene="phoA"
                     /locus_tag="OO609_003462"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6634747.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2123..2383)
                     /gene="iraP"
                     /locus_tag="OO609_003463"
     CDS             complement(2123..2383)
                     /gene="iraP"
                     /locus_tag="OO609_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6634748.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2565..2768)
                     /locus_tag="OO609_003464"
     CDS             complement(2565..2768)
                     /locus_tag="OO609_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634749.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2846..3940
                     /gene="ddlA"
                     /locus_tag="OO609_003465"
     CDS             2846..3940
                     /gene="ddlA"
                     /locus_tag="OO609_003465"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6634750.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3964..4176)
                     /locus_tag="OO609_003466"
     CDS             complement(3964..4176)
                     /locus_tag="OO609_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6634751.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4436..4744
                     /locus_tag="OO609_003467"
     CDS             4436..4744
                     /locus_tag="OO609_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6634752.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4803..5897)
                     /gene="yaiW"
                     /locus_tag="OO609_003468"
     CDS             complement(4803..5897)
                     /gene="yaiW"
                     /locus_tag="OO609_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6634753.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5910..7130)
                     /gene="sbmA"
                     /locus_tag="OO609_003469"
     CDS             complement(5910..7130)
                     /gene="sbmA"
                     /locus_tag="OO609_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6634754.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7482..8639
                     /gene="ampH"
                     /locus_tag="OO609_003470"
     CDS             7482..8639
                     /gene="ampH"
                     /locus_tag="OO609_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6634755.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8640..9143)
                     /locus_tag="OO609_003471"
     CDS             complement(8640..9143)
                     /locus_tag="OO609_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6634756.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9231..>9757)
                     /locus_tag="OO609_003472"
     CDS             complement(9231..>9757)
                     /locus_tag="OO609_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6634757.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2429 a         2488 c         2328 g         2512 t
ORIGIN      
        1 acgtgggtgg actggtataa caatcgacga ttgcaggaaa ggctgggcca catccctccg
       61 gcagaagcag aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt
      121 cacagataaa acactctcca ggaaacccgg ggcggttcaa tcaggtatca ggcggctttc
      181 ttgaggcagg cactcataaa cttattacga tcatcacctt tcagagcttg ttgcgttgct
      241 tgattattac attcacgcat cttttgctgc tgtggcgtca aacttttttc accaggcgca
      301 gacttgctgt tcttcaggca atcactcatg taggtcttac gagcatcccc tttcaacgcc
      361 tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt gttgtggggt taaggttctc
      421 tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa gcaaggtaac cagtaatgtt
      481 attttcatag caccatccct cttcatgttt taaccatgag cgtttgcgcc cgtgatctgc
      541 cattaagtct ggttgctaac agcaaaaaaa ccacccggca gcgaaaattc actgccgggc
      601 gcagttttat ttcagcccca gagcggcttt catggtgtag aagagatcgg tctggtcggt
      661 cagtccaaca acattggcgg catgcgggcc atacgccgca atacgcaact gactgccggt
      721 atgttcttgt gaatcctctt cggaattccc gtaactcatc accatcactg cgccatcttt
      781 agtatttagc gcctgagtga ggcccggcgc tttggtatcc ggcgcaacaa cctggctggc
      841 gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc tcctttttag cgaattccag
      901 cgcccgttgt acggcttcat cgagatcgac cgtctcgcca atttgcccac aagggttcgc
      961 agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc aggaaaaatc ctttctcatt
     1021 tttactcaac aattcaatgg ctttgtcggt catctgtgcc agggttggta cactgtcatt
     1081 acgttgcgga ttaggcgtat aggtgactgc gggcttatcg atattgccgt ggtacgttgc
     1141 tttcggccct agccagcgca ctggcatatt gccgtcagca aacaggccaa gcaggggttt
     1201 ttgctgattc gcttccgtca ccgaattcag tgaggcagca tcgttcacca actgataacc
     1261 acgcgcctgt gcctgttcac gcagcgtttt tccctgccat tcaccagcgg ttgccgtttc
     1321 agcaaaggtt tttgcgccgc cgccaagcgt aacgtcggca cgagcgttaa gcagctgttc
     1381 ggtaatcgat ccttttccgc ctttttccag agcgttaccc ggacattttt cactggtcgc
     1441 gctcggaccg tagcatttgc gcgaagtcac atgtgccacc agcgcagcgg gcgtggcatc
     1501 ctgcaactct gcggtagaaa cgttaccggt cgccagacct gcggcttttg ccatttccag
     1561 aatcgttggg tgatcttttt cgtgaatatc gacgcccagc gcgccgttat aggttttgac
     1621 atcggttgac caggcggttg ctgatgcagc cgagtcggtg acgtagtccg gtttgccagt
     1681 ttttttattc agcgcatagt gagtgtattg cccggtaagc ggtaaggcat cgataccttt
     1741 aaaaaagccg cccgcacctt cggcataatt acgtgcggca gtaatttccg aatcccccat
     1801 cccatcgcca atcagcaaaa taatattttt tgcaggttta tcgctaagag aatcacgcag
     1861 agcggcggtc tgatcacccg ttaagcggcg agcaccgccg ggtgcagtaa tatcgccctg
     1921 agcagcccgg ttttccagaa caggcatttc tggtgtccgg gcttttgtca caggggtaaa
     1981 cagtaacggt aagagtgcca gtgcaatagt gctttgtttc actttatttt ctccatgtac
     2041 aaatacatta aaaaataaaa acaaagcgac tataagtctc ggccgtgaca actttatgac
     2101 agctgttgaa aagattaact ttttactgac aaggatgctt caataacttc tttacgtaat
     2161 cgcgcagcag ctctgtatcg tcgtcaggaa tgctggcatc gggctttacc tcgtacagcg
     2221 ccccctctac ctgatcaatc aaccgctgtt ggtcattttg cgccatattg cgaagcattg
     2281 cagtgacgat aatctccaaa gcttctacct gcgcacacag ttctttcgac tcttcttctt
     2341 tttgggcaag cttaaataac aactcagcaa tgagattttt catgtctgta tttccttatc
     2401 caaagtatgg agaagttaac attatgtttc acaacttcat agtgtctgga agtattattt
     2461 ttatgacaaa ggaaatactt tgcgcaaata ttttttgttt gattgccagc gaaacgtttc
     2521 tcttatttaa ataattaccc acacaaaata taggctttta attatcaacc tttattataa
     2581 ataaaagcta tgaaatatat tctcttcgtt aacttataat taagagaaaa aaactcaccg
     2641 cattgccatt taagccagga ataaattagc ttcccgttca tcatttttgt gatcttgcgc
     2701 acatctttga gataacgcac tttcttcacc agtacgttgc acgctttgtc agcagaagat
     2761 tgcaacaatc tttcagaagg ccgcgaatcc cccgttttgt cgtggtaatg tatgcacttt
     2821 gtgttagacg ggataggttt ttaagatgga aaaactgcgg gtaggaatcg tttttggtgg
     2881 taaatcagcg gaacatgaag tgtctctgca atcggcaaaa aacattgtcg atgccattga
     2941 taaaagtcgc ttcgacgttg tgctgctggg cattgataaa caagggcaat ggcacgtcag
     3001 cgatgccagc aattatctgc taaatgcaga cgatcctgcc catattgcgt tgcgcccttc
     3061 ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat cagcttatcg acgcgcaaaa
     3121 cggtcagccg ttgccgacgg tggatgtcat tttcccgatt gtccacggta cgctgggcga
     3181 agatggttcc ttgcagggaa tgctgcgggt cgccaattta ccgtttgtag gttctgatgt
     3241 tctggcttca gcagcctgta tggataaaga tgtcaccaaa cgtctgctac gcgatgccgg
     3301 gctgaacatt gcgccattta ttaccctgac gcgcgctaat cgtcacaaca tcagttttgc
     3361 cgaagtggag tctaaactgg ggttaccact gtttgtaaaa ccggctaatc agggctcttc
     3421 tgttggtgtc agcaaagtaa ccagtgaaga acagtacgca attgccgtcg atctggcgtt
     3481 cgagttcgat cataaagtga tcgttgagca agggatcaaa ggtcgtgaga tcgaatgcgc
     3541 agttctgggc aacgacaatc cgcaagccag cacctgtggc gagatcgtac tcaccagcga
     3601 tttctatgcc tacgacacca agtacattga cgaagatggc gcgaaagtgg tagttccggc
     3661 agccattgca ccagagatca acgataagat ccgggcgatt gccgttcagg cttatcagac
     3721 gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta accccagaga acgaagtggt
     3781 gatcaacgag atcaacacac tgcctggctt caccaacatc agtatgtatc cgaagctgtg
     3841 gcaagccagc ggtctgggtt acaccgatct gatcacacgt ttgattgagc tggcgctgga
     3901 gcgtcacgct gcggataacg cattgaaaac cacaatgtaa tattttaacg cccgataata
     3961 ttcttattgg gcgttttcag tttcttcatc gcgacgacga ataatcaacc ccgccagcca
     4021 gaaactaatc acccacgtca ccagaccgac ggcataggtc tgccaacctt ttgcttcaaa
     4081 tcccagtacc cccaccacgc cattaagaat gaatataagg ccaatggcga acgcatagta
     4141 gtgccagtca cggcggattt ttacaggcag attcatggca gctccgttaa cgaaaaacaa
     4201 catccttgca tattctgtaa caaccgtctg tggtgtagac aacattttcg taattattaa
     4261 taaatttaac ttaaggttgc cattttgtga ctcacaacga agattcacgc ttcgggaata
     4321 ttcccaacat caatttacca tgattctgat attgacaatc taaatgacct gtcagacttg
     4381 ccataagccg ctggtatcac accagaagag aagcagatga cttccggagg tttttatggc
     4441 tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat cgcaatgcct cttcaacgcc
     4501 tggcaacatt gcctatgcgt tgtttgtgct gttttgcttt tgggctgggg cgcaattgct
     4561 gaacctgtta gtgcatgcgc ccggcgtcta tgagcgttta atgcaggtcc aggaaacagg
     4621 tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt ttcgggctga tcccgttttt
     4681 agcaggctgc ctcatttttg cagtggtggc gctatggctg cactggcgac atcgccgcca
     4741 gtaaaattta cgttgccaga tgcggcctga aacgccttat ctggcctgac attcctgttt
     4801 gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc gcttcgcaaa ccaggccgta
     4861 gtcaggttgc gggtgatttt cgggctttcc agttgaatgc caggcaacat ttcgcggggt
     4921 agtgatttac ccgttttcgc ttcggcaagt tgataaactt tcttgtacag cgccgtctcc
     4981 tcaaaagaga agctgtcacc tttctctaac tgacggcgaa tttcgctgtc gctcatcccc
     5041 agttttcccg ccagtttgcg cgttgccagt tcggttttcc cgggttcttt actgtcgtaa
     5101 cgaattaaat caccgtccag tgccagcttc acgccgctgg ccttactgac tgcgttctga
     5161 aacgcggcat tacggctggc gtaccagcca gcgttaaaat cagcaaagcg gtatatcggt
     5221 gcgctatagc tggcgggata attcagtaaa tggtaagtac caaaccacaa cccgccgcgg
     5281 cgactgaaga cttcctgacg aactgtaccg tccattttcc acggataccc tttggtatgc
     5341 tgttcggcaa aagcaatgct gacctgcatc ggcccaccgg tgcgcaccgg attaagcgaa
     5401 ccaaacagcg tctgccccat tggtaccatg ctgatcagat cgtcaaaaat cgcgctcaat
     5461 tgcttttctg tacgcaccga atctaaccgt tcgctataac ttttgccgtt tggcgatttg
     5521 atttttagcg cggtatgaac cagaaaagca ggaatgtgca tccgttcagc acgacggtca
     5581 atctcttgcc aggcgatctt gcttaagccc ggaacggccg gatctgcctg ataatttgac
     5641 tcctgctgcg ccaccgccag caccgagtag acgttttcca gcgtcggtgc cagcccctga
     5701 ctttcaaagg tggtggcgag atctttcgcc caggcatcgc ggtctttcac gctcgcaggc
     5761 atcttctgcc gcacgacgct cgctacatct atcgcttttt cgcccttttt caacggttgc
     5821 ggagcctgac tactacaccc cgctaaaacc agaaccgcca acaatgacag cgaagataga
     5881 tgattgacgc gcgacattac ttctcctttt tagctcaagg tatgggttac ttcctgaatt
     5941 ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt tgtagataga catcaactca
     6001 accagtgtgg tccatgagtt aatcaggtac tggaaagcac cgcgaacctg accaaaaacg
     6061 ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg caacaattga cggaaacagc
     6121 aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa tgcgggcgat gttgaaatac
     6181 atatagtgaa aatagaggcg aaaatagttt ttccgtacgg cgctaaacag ctcgcgtacc
     6241 gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa ccagctcttt acggtaggcg
     6301 gcctctactc gctggttttt aaactccagc cccggcagtt tgatccctac cactgccagc
     6361 aatccggtcc ccatcagcga ccagacgata gcggcaatca ccagaccata cggaatgtgc
     6421 ccgataatcg gcagctccgg cacatgcgcg gagagcgtta ccagcaccgg caggaaggcg
     6481 atcaacgtca tgatggcgtt gataaaactg acgcccatat tctccagcgt tgaagcaaaa
     6541 cgcatggtgt cttcctgcac acgctgtgcg gcaccttcga tatgacgcag ttgttgccag
     6601 ttcgccatgt aatattcgtt catcgccgta cgccagcgga acacgtagtg actgacaaag
     6661 aagttgttca gtacactgat caccaccgcg atcagcgcaa tccccagaaa gacgcccact
     6721 tcgcggtaaa attgttcgat agtgacttta tgcggcgaac ttagcgcggt ttgaatcaga
     6781 tcatagaacg gcgcatacca ggcgttgacg gcgaccccga cttccaccaa aaaccaagtg
     6841 acgaagataa tcagcgcagt accgagtatt gaccagtatt gccaacggtg cgggctgtag
     6901 ataaaccaga aaaatgcaaa aagtcctacg caaacaatgt agtaagcgta aaaaatcagg
     6961 aaatccaacg accagaaacg cgcggcgcta atcgggatct gcccggaagc gccggtgata
     7021 cgtgccaccc agtcaccccc accggcttgc cagaagataa cggcaatcaa tgcccaaaca
     7081 aaggccgaga gaaaaaacgt tcccggcttt gggaaaaaag acttaaacat atgcactcct
     7141 gctaacttct tatcgtttta acaactgctg tgtattaacc gcatgaccgc tacaaagcgt
     7201 cagccaaatg aaaccgccca ttgtgaccca tagcaaggcg cttagttcgc cagcctgacc
     7261 ataagaattg tttcgacgga tttcacaaac caaagattgt ttacataagc ggattaacac
     7321 gcgcaggatc acaaatgcag gcattggtga gcaggaaaaa gatcgtgttt acgcgccgtt
     7381 acgtttttaa cactattatt gcactaattc gccctttgca atccatcaat gagtagtata
     7441 aatacgctca gttaccttca ttcaatctat ggacaccacc gttgaaacgt agtctgcttt
     7501 tttctgccgt gctgtgtgcg gcgtcattga cctccgtcca tgcggcgcag ccgatcactg
     7561 aaccggagtt tgcctctgat attgtcgatc gttatgccga tcatattttt tacggcagcg
     7621 gtgccacggg gatggcgctg gttgttatcg acggtaacca gcgcgtcttt cgtagttatg
     7681 gtgaaacgcg acctggtaat aacgttcgcc cgcagctgga ttcggtcgtg cgtattgctt
     7741 ccctcaccaa actaatgacc agtgaaatgc tggtgaaatt gctcgaccag gggaccgtaa
     7801 agttaaacga tccgctaagt aaatatgctc cgccaggcgc acgcgtgccg acctacaatg
     7861 ggacgcccat taccctagtg aatctggcaa cccataccag cgccctgccc cgtgaacagc
     7921 ccggtggcgc ggcacatcgt ccggtgtttg tctggccaac gcgcgagcaa cgctggaaat
     7981 acctttctac ggcgaagctg aaagccgcgc caggcagtca ggcggcgtac tctaaccttg
     8041 cgttcgacct gctggcagat gcactggcga atgcgtccgg caagccttat acccagttgt
     8101 ttgaagagca gattacccat ccattgggga tgaaagacac cacctacacc ccctcaccgg
     8161 atcagtgccg ccgtttaatg gttgctgagc gcggtgccag tccgtgcaat aacacgctgg
     8221 cggcaattgg cagcggcggc gtctattcca cgcctggcga tatgatgcgc tggatgcagc
     8281 agtatttgtc atctgatttt tatcagcgca gtaaccaggc agatcgcatg cagacgttga
     8341 tttatcagcg cgcgcagttt acgaaagtga ttggcatgga tgtccccggc aaagcggatg
     8401 cgcttggttt aggctgggta tatatggccc cgaaagaggg tcgtccgagg attattcaga
     8461 agacaggcgg tggtggcggt ttcattacct atatggcgat gatcccgcag aagaatattg
     8521 gcgcgtttgt ggtggtcact cgttcgccgt tgacgcgctt taaaaatatg agcgatggca
     8581 tcaatgatct ggtgaccgag ctaagcggga ataaaccgtt ggttatcccc gcatcctgat
     8641 taatactctg aaggcaaacg gttgatagcg accagtttgc ctttattcat ttcgatatag
     8701 ccgcctttac gcaaagcagc gagaacttct gcgacgacag aacgcgatat gcgtgtacgt
     8761 tgatggatat aattcatcac accaatacgt gatcgcaatt cttcattcca gtcaatcatt
     8821 gataataatg ttgcgcgggt ttgtttggct ggtagatgat atcccgtaca atttccttct
     8881 gatattaatt tgtatggtat atcgtttttc attaaaccat cagccagccc cataatataa
     8941 ggagcctggg taataccgat aagtacgttt tcttctctac gcagagagat aactccttct
     9001 aaaataacga aagtatcttc gttattgaca tcactggaaa atataacttg cttttcatta
     9061 ttaaactcga agcgcgtacc gtatctggac aaacatttat cgagcttacc aaattcctga
     9121 agaggtttaa ctacagataa catttgcgcg tcctttgcag taatgcccgt caaatccttg
     9181 acgggcatta tttagattaa attaccagta tttcttcgga gtgaagaata ttaccaagta
     9241 tatttaacac ccacgttcgc ggaccagtct tgatctacgt caccaccacc gaggtagtta
     9301 gcatcggtat aggcgctgaa gttcttggtg aagctgaact gagtacccag accaacacgt
     9361 accgcagacc cttcagtacc gttatcgatg gaatcaccgt tcacatcgtt atcgttgtta
     9421 gagtcgtcgt agacgtaagc cagtttgaag tacggagtca gagcctgatc ttcgctgtag
     9481 gtgaaggtat aacctgcatc tacacccagt tcataacgca tgctgtcgta aggctgaccg
     9541 tcaactttca tgtcgttgct cagctggtag tcatcaccag actggaacag accagaaatg
     9601 ctgccgtaag gagtcacgta accagcatca cccagtttga agtcgtaacc ggttttcaaa
     9661 ccgaagcccc aagcgtcgga gttggtgcta ccgtcaacgt aagtaccgtt gctcatggtt
     9721 gcagacaggt cgttgttgaa gtgagagtag ctcaagc
//
LOCUS       DAJTYH010000133         9655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTYH010000133 DAJTYH010000000
VERSION     DAJTYH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..741)
                     /gene="bglB"
                     /locus_tag="OO609_003473"
     CDS             complement(<1..741)
                     /gene="bglB"
                     /locus_tag="OO609_003473"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6634758.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEE"
     gene            complement(760..2637)
                     /gene="bglF"
                     /locus_tag="OO609_003474"
     CDS             complement(760..2637)
                     /gene="bglF"
                     /locus_tag="OO609_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6634759.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2771..3592)
                     /gene="bglG"
                     /locus_tag="OO609_003475"
     CDS             complement(2771..3592)
                     /gene="bglG"
                     /locus_tag="OO609_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6634760.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(3879..4604)
                     /gene="phoU"
                     /locus_tag="OO609_003476"
     CDS             complement(3879..4604)
                     /gene="phoU"
                     /locus_tag="OO609_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6634761.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4619..5392)
                     /gene="pstB"
                     /locus_tag="OO609_003477"
     CDS             complement(4619..5392)
                     /gene="pstB"
                     /locus_tag="OO609_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6634762.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5484..6374)
                     /gene="pstA"
                     /locus_tag="OO609_003478"
     CDS             complement(5484..6374)
                     /gene="pstA"
                     /locus_tag="OO609_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6634763.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6374..7333)
                     /gene="pstC"
                     /locus_tag="OO609_003479"
     CDS             complement(6374..7333)
                     /gene="pstC"
                     /locus_tag="OO609_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6634764.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7420..8460)
                     /gene="pstS"
                     /locus_tag="OO609_003480"
     CDS             complement(7420..8460)
                     /gene="pstS"
                     /locus_tag="OO609_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6634765.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8708..>9655)
                     /locus_tag="OO609_003481"
     CDS             complement(8708..>9655)
                     /locus_tag="OO609_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6634766.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2486 a         2520 c         2236 g         2413 t
ORIGIN      
        1 ctcttccatg gcctgcaaca tatcctgtgg ctggcaggtg agggggtaaa ccagcccacc
       61 gagaagcata ttgccgattt tcgcttcggg gatcaggcta tgataggctt taactgcccg
      121 cgcactggca accagttgat gatggatcgc ctgataaact tccgcctcgc cactctcttc
      181 tgccagcccc acgcccgtga atggcgcgtg taacgacata ttgatttcat taaacgtcag
      241 ccataacgct actttatgtt ggtagcgggt aaagaccgtg cgggcgtaat gctcgaagtg
      301 atcgatgacc gcccgattag cccaaccgcc gtagtttttc accagcccat atagcatttc
      361 gtaatgggat aacgttacca gcggcttgat ccccgcctgc gccatttcat caaacagccg
      421 atcgtaaaac gctaaccccg cttcattcgg ttcggcttcg tcgccctgag ggaaaattcg
      481 cgcccaggca atggaaatac gcagacaggt gaagcccatc tcggcaaata acgcgatatc
      541 ttccgggtaa cggtaataaa aatcgatggc gacatctttg atattctctt tccccaggat
      601 gcgcggttcc atttttccca ttacgccatg aggctgtaaa tctgaggtcg tgatcccttt
      661 gccatcttcc agccaggcac cttccacctg attggcagct gttgcgccac cccaaagaaa
      721 tgtttctgga aatgctttca taattaactc cttttatcgt tagcgaatga tggataacag
      781 cggttcacct gcgcttatct gcgccgtgcc gtggggtaat acgtctgtaa aatcatcgct
      841 attactgatt aataccggcg tcgtcagatc aaatccggcc tcgcgaatag cagggatatc
      901 aaaagaaatc agccgatcgc ctgtattgac cttgtcaccc acgttgacgt gagcggaaaa
      961 gaatttgccg tccagtttta cggtgtcgat accgacatga atcaggatct ccacaccatc
     1021 atctgactca atgccaatgg cgtgtaatgt ggcgaacaac gaagcaattc gacccgcaac
     1081 cggagaacgc acttcaccaa ccgagggcag aatggcgata cctttaccca acaggccact
     1141 ggcaaacgtg gtatcagcga cgtgaatgag cggcacaatc tctcccgtca tcggtgaaca
     1201 gataccgccc tgctcaggtg gtgtaataac ctctggtgtt ttctcttgcg gggcaccctg
     1261 cgctggctga cgtttactgg tgatgaaatg aagcatcaac gtaccaacaa atgcgcaacc
     1321 tatggcaatg acaccgccaa taacgctggc ccagacggtg aaatcaatcc ccgttgacgg
     1381 gatggtctgc atgaaggtga aaatacttgg caaaccaaag gagtaaactt tcgtttgcgc
     1441 gtagccaatg atggtggccc ccaatgctcc actgatacag gcgataacaa aggggtactt
     1501 acgcggcagg ttgacgccat ataccgctgg ttcggtgata ccaaacagac ccgtcaacgc
     1561 cgctgatccc gccaccactt ttttctgcgc atcgcgttcg cagaggaaga cgccgagcgc
     1621 cgccccgacc tgcgccataa tggcgggcat taacagcggg atcatggtgt cgtagcccag
     1681 cacggtgaag ttattgatac acagcggcac caggcccctg tgcagtccga acatgacgaa
     1741 gatttgccag aagccgccca ttaccgcgcc cgcaaatgca ggaaccgcct gataaagcca
     1801 gagataaccg gcggcaatca tttcgcttat ccaggttgat agctgcccca ccagcagaaa
     1861 ggtgacgggt gtgataacca tcagacatag caatggtgtg aagaaatttt tgattgccga
     1921 cggtaaccac gcattaagtc ggcgttccag aatgctgcac aaccaggcag aaaaaataat
     1981 gggaataacc gatgacgagt aattcaacaa tgtgaccgga atacccagga aatccagccc
     2041 cggcgcatcc gctttttgcc cgttctcgaa ggcagtcaga attaatggat gcactaacgc
     2101 tccaccaatc accatggcag taaatggatt accgccgaag cgtttgcccg cggtgtatcc
     2161 caggattatc gggaagaacc caaacaaggc atcactggcg ctgaataaaa ttaaataagt
     2221 accactttgt tcggtcgtcc actgaaaagt gagcgccaga gccagcatac ctttcaggat
     2281 cccggttgcc gccatcagac cgatcagagg cgtaaaaata cctgaaataa cataaacaaa
     2341 gcggtttagc agattacctt tctcatcatt ttccggtgcc tgttgcgctt tttcgccaag
     2401 gcctgccacg ctgttaaccg ccaggaagac atcggccaca tggttaccta tgaccacctg
     2461 aaactgacca ccgctttcca ccaccataat aataccgggg gtctttttca gtacctcagc
     2521 ttgcgctttg ctttcatcct ttaacttaaa acgtaatcgc gttgcgcaat gcatcagact
     2581 cacaatgtta tctgcgcccc cgactcctgc gactattttt ctggctaact ccgtcataac
     2641 ttgccctcta ccgctttgcg gcaaaactcc aaaaaaaaac ctgaaaaaaa cggcctgacg
     2701 tgaatcaagc aatttttttc aggttttgcc cgcttagtgc ggtaacaatc ctttactcag
     2761 taataatatt tcagtgttct ttgcgcacgc gctctatatt tatggctaaa aacataatct
     2821 ctgcgggtga aattttacgt tgatactgca aaccaataaa aatggcgatc cgttccgcac
     2881 attgccatgc ttgcgggtaa ttttgtttta ctgcttgttg taatgattca tcactatcgt
     2941 taattgaagc atgttcaaga atacgccagg ataaaaactt cagatgtgta accagtcgct
     3001 gataactcaa gctttcttcc tggtaattaa ggctgaactg aaattttatt aattgcagca
     3061 tttcgcgcat taactgcgtg acacctgcaa catcctccat atttccgctc atttgggcac
     3121 tgaccagatg catggcaata aagcccactt catctttcgg taactgcacg cccaaccgtt
     3181 tatcaataat ggttaacgct tcttccccta gctggaactc tttcgggtaa agccgctgga
     3241 tatcccacag caacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
     3301 gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataagac
     3361 gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt
     3421 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
     3481 cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
     3541 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
     3601 ccttttttat aaacaaaaaa aaaccgactt caccagtatt ctctggttat gtcaggtttt
     3661 gcctgcgaat gcagtaacaa tccagtcatt tattaatggt ttttataacg aacatccagg
     3721 ttcggaaatt aatttaattg cgtgcagaga aaatagcaat gcgctattga taaaaatatg
     3781 accatgctcg cagttattaa ccttgtgtaa ttttaggaat ttataaagtt atatataaca
     3841 aatcccaata attaagttat tgggatttgt ctggtgaatt atttgtcgct atcttttccc
     3901 gccagcagtt tatccagctc atcaccgcct acatgacgga aatcctgacc cttcacgtag
     3961 tagaagataa actcacaaat attctggcag cggtcgccga tacgttcgat tgaacgcgca
     4021 cagaacagcg cggtaagtac gctcggaata gtgcgcgaat cttccatcat gtaggtcatc
     4081 agttgacgaa caataccttc gtattcctga tcgacttttt tatcttcacg ataaatacgt
     4141 accgcttcgt caatgtccat ccgcgcgaac gcgtccagca cgtcgtgcag catctggatg
     4201 gtatgacggc ccagcgactc cagacttacc agcaacggct gatgctgctg ggagaatttc
     4261 tccagcgcag tacggcagat tttgtccgcc acgtcgccaa tacgctccag ctcggcaatg
     4321 gttttactga tcaccataac cagtcgcagg tcgctcgccg tcggctgacg tttggcgata
     4381 atgcgcacgc aggcttcatc gatcgccact tccatcatgt tgacgttctt gtcgccttcg
     4441 atcacgcgct tcgccagatc gctgtcctgg ttatgcatcg cggtgattgc atcagaaagc
     4501 tgctgctcca ccatgccgcc catggtcatc acctgcgtgc ggatactttc cagttcggcg
     4561 ttgaactggc cggaaatatg tttattaaga ttgagactgt ccataacgca ctcctgaatc
     4621 aaccgtaacg accggtgatg tagtcttctg tttgtttctt cgctggcttg gtgaacagat
     4681 cgtccgtgtt gctgaactca atcaattcgc ccaggtacat aaatgccgtg tggtcggaac
     4741 aacgcgcagc ctgctgcatg ttgtgggtga cgatcaccac ggtgtaatcc tgcttcagtt
     4801 cggtgatcag ctcttcaata cgcccggtag agatggggtc gagcgccgaa cacggttcgt
     4861 cgagcagcag cacttccggg cgaatggcga taccacgcgc aatacacaga cgctgttgct
     4921 gaccaccaga gagagagtaa ccgctctggt gcaatttatc tttggtttcg ttccacaatg
     4981 cggctttggt caatgcccac tgcacgcgct cgtccatgtc ggcacgggag agcttctcaa
     5041 acagacgaac gccaaaggcg atgttgtcgt agatggacat cgggaacggc gtcggtttct
     5101 ggaacaccat gcccactttc gcacgcagca gggcgatatc ctgagagttg gtcaggatgt
     5161 tgtcgccatc aagcagaatt tcaccttccg cacgctgctc cgggtacagt tcaaacattt
     5221 tgttgaaggt acgcagcagc gtcgatttac cgcagccgga cggcccgata aacgccgtaa
     5281 cctggttttt ggcgatatcc aggttgatgt ttttcagggc atggaatttg ccgtagtaga
     5341 agttcaaatt acgaacctga attttactcg gggcagtttc aaccatactc attgcaatct
     5401 tttcctcatc gtacctgatg cttttcgcta atcaggctta ccgtagggcc gggtaaggcg
     5461 ttcacgccgc atccggaaaa gtgttaaccg tgtttattct tcgcaaaaac aacgcgcgcc
     5521 agaatgttca gcagcaggac gcacagggta atgatcaata ccccggccca ggccagttgt
     5581 tgccattccg caaacgggct catcgcaaac ttaaagatcg tcaccggcag gttggcgatc
     5641 ggctgcatca tgtccgtgct ccagaactgg ttggagagcg cggtaaacag cagcggcgcg
     5701 gtttcaccgg caatacgggc aatcgccagc aggataccgg tcataatccc ggacaccgac
     5761 gctttcagcg taatcgcgga gatcatcttc cacttcggtg tacccagcgc ataagccgct
     5821 tcacgcaggc tgtccggcac cagtttcagc atgttctcag tggtgcggat aacgatcggc
     5881 acctgcaaca gcgccagcgc aattacgccc gcccagccgg agaagtgctc catctgtgcc
     5941 accacgatgg tgtagacaaa cagaccaacc acaatcgacg gcgcggagag cagaatgtcg
     6001 ttaatgaagc gaatcacttc tgccagccag gatttacgac catattccgc cagataaatc
     6061 cccgccataa tgcccagcgg cgtaccgaat accgtggccc acaaaattaa cagcccgcta
     6121 cccgccaggg cgttcgccag accaccacct tccgtattgg gcggcggcgt catttcagtg
     6181 aacagcgcca gcgacatacc gtcgataccg cgagtgatgg tggacattaa aatccagatc
     6241 agccagaaca ggccgaaggc catcgtcgcc atcgagagcg tcagcgcaat acggtttttg
     6301 aggcggcgac gcgcctgcat tttgcggcga gattcagcca gcgccgcagt ggtttgcatt
     6361 tcaaccatag ccattagcgt gccccctcat tcttagccag gcgcataatc ataaacttcg
     6421 atgcggcgag gacgatgaag gtaatcacaa acaggatcag gcccagttcc atcagtgcgg
     6481 caacgtgcag accggattcc gcttccgcaa attcgttcgc cagcgcagag gtgatactat
     6541 tgcccggcat gtacagcgag gcgctgtcga gctggtaggt gttaccgatg ataaaggtca
     6601 ccgccatggt ttcaccgagc gcgcggccca gccccagcat gatgccgccg ataacaccat
     6661 ttttggtgaa cggaagaacg atacgccaga taacttccca ggtggtgcag ccaataccgt
     6721 aggccgactc tttcatcatc accggggttt gttcgaacac atcacgcatt accgccgcaa
     6781 tgtacggaat aatcatgatg gcgaggatca cgcctgccgc gaggataccg ataccaaatg
     6841 cggggccaga gaacagcgcg ccaacaatcg ggatattcga catgatattg ccgaccggct
     6901 cctgaaagta aacggcgaac agcggcgcaa agataaacag gccccacatg ccgtaaacga
     6961 tacttggaat ggctgccagc agctcaatgg cgatacccag cgggcgtttc agccagccag
     7021 gcgcaagctc agtcaggaac agggcgatac cgaaactcac cgggacggcg atcagcagcg
     7081 cgataaacga agtcaccaac gtaccgtaga tcggcaccag cgccccgtag atatcgttcg
     7141 gtgcatccca ctctttggtc cataggaaag ccagaccaaa tttctgaatg ctcggccagg
     7201 aggagatgat cagagagaca ataatgccac ccaacatcaa tagcacaatc agcgccgcca
     7261 gttttaccag cacgctgaaa attatgtcgc cctttttacc cggtgggtta aaagcgggct
     7321 tggttgcagc cataagttac tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc
     7381 ggatgcggcg taaaacaccg tacccggcct ggagttttat tagtacagcg gcttaccgct
     7441 actgtcttta atattggtct tccacgcagc gcgaacctgt tcaactacac tatccggcag
     7501 gctggcgtaa tccaggtcgt tcgcctgttt agccccggtt ttgtacgccc agtcgaagaa
     7561 tttcagcacc tctgtgcctt gttctggttt cttctgatct ttgtggatca gaatgaacgt
     7621 ggtagaggta ataggccatg catcttcgcc tttctggttg gtcagatcct gagcgaaggt
     7681 tttgctccag tctgcacctt ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg
     7741 tttaccatca gcggagatca gtttggtgta cgccaggttg ttctgcttcg cgtaagcata
     7801 ttcaacataa ccaattgcac ccggcagacg ctgaacgaac gcggcgatac cgtcgttacc
     7861 tttaccgccc agaccgatcg gccattttac ggtagagcca gtaccaacgt tgtttttcca
     7921 ctcttcgttc actttcgcca ggtagctggt gaagacgaag gaagtcccgg agccatctgc
     7981 gcggcgtact acagcaatgt tttgtgaagg cagtttcaga cccggattca gtttggcgat
     8041 ggcttcatca tcccacttct tgattttgcc caggtagatg tcgccgaggg ttttaccatc
     8101 cagcaccagt tcgccagact tcagccctgg aatgttaacc gccagcacca cgccgccaat
     8161 cacggtcggg aactggaaca gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc
     8221 agaggcacca aaatcaacgg tattagcgat aatctgtttt acgccaccgg aagaaccgat
     8281 accctggtag ttaactttat taccggtttc tttctggtaa gtgtcagccc atttggcata
     8341 caccggcgca gggaaggttg cacctgcacc tgtcaggctt gcttctgcaa acacagagaa
     8401 agcactcatc gataaggtcg cggcgacaac agttgcgacg gtggtacgca taactttcat
     8461 aatgtctcct gggaggattc ataaagcatt gtttgttggc tacgagaagc aaaataggac
     8521 aaacaggtga cagttatata taaggaatat gacattttta tgacagaaag gaattaccaa
     8581 ataacttact gattttaaat gtgaagatga aaattccaca tgccatcttt ttgtcatgat
     8641 gcatttaaaa gtgataaaga aatgggtgag atgacaacga tgtgatgaac aatcgttgtc
     8701 agacctatta gcgcacaatc tcaacagtta tcgtcgctgt tgcagtaaat tgccccgggg
     8761 ctgggcgagc tccagtaagg ctcgcaggag cagctgaaaa agttacacta cctgattttt
     8821 caccatacac tgttgattta tccatgtctg ttggcaactc accaccgttc actttgattt
     8881 ccttattatt tttgtcgtaa atttttatcc ccacgttaga gtttgaagtt acgaccattg
     8941 aattgtccgt attgggttcg gtagcataaa aagacgtact gatatcatcg gtattcagta
     9001 tccccaggtt ttcacactca atagtgactg ttttagtaat tttatgtgtt gttaccgcac
     9061 ttccaacaac agatgaaaaa tctgcagctg gtatttcttt aaaatcaaaa caaactggct
     9121 ttaaattatt tatttcgcaa ctttgcggag ctgtaatatc gccccgaata taaacatcag
     9181 ccatcggctg tgagtaatcc accggggtgt cacggctaat tgttccatac aatgtcacta
     9241 tctttgtttc aggaataatc aattttcctg caaatgtctt cttgatataa aaatatacta
     9301 tcgctttagc cccactggat atacttaaag gttcaatgcg gttgcaattg tactttgtat
     9361 taccagacgg gttgttagca tgatattcaa aaggtgcatt cacataccca accccaagaa
     9421 tatctatagc aatcccaaca tcaaggttcg gattaagatc ataataatta atattagagt
     9481 gaaccccagc ataagttaat gcctggttta tttttgacgt ataatagata taggggaatt
     9541 ctcctacgtt accagtatca caatgacagc gtccaagata tgaggatcca ttagttacag
     9601 tggtgttaaa tgtcgttcca accttatttt cgttcgcggt aataagatat cccga
//
LOCUS       DAJTYH010000134         9532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTYH010000134 DAJTYH010000000
VERSION     DAJTYH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..603)
                     /locus_tag="OO609_003482"
     CDS             complement(<1..603)
                     /locus_tag="OO609_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR6634767.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRVM"
     gene            complement(670..1395)
                     /gene="fimC"
                     /locus_tag="OO609_003483"
     CDS             complement(670..1395)
                     /gene="fimC"
                     /locus_tag="OO609_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6634768.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1432..1971)
                     /locus_tag="OO609_003484"
     CDS             complement(1432..1971)
                     /locus_tag="OO609_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6634769.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2036..2584)
                     /gene="fimA"
                     /locus_tag="OO609_003485"
     CDS             complement(2036..2584)
                     /gene="fimA"
                     /locus_tag="OO609_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6634770.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3065..3661)
                     /gene="fimE"
                     /locus_tag="OO609_003486"
     CDS             complement(3065..3661)
                     /gene="fimE"
                     /locus_tag="OO609_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6634771.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4139..4741)
                     /gene="fimB"
                     /locus_tag="OO609_003487"
                     /pseudo
     CDS             complement(4139..4741)
                     /gene="fimB"
                     /locus_tag="OO609_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6195..6911
                     /gene="nanC"
                     /locus_tag="OO609_003488"
     CDS             6195..6911
                     /gene="nanC"
                     /locus_tag="OO609_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6634772.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6931..8037
                     /gene="nanM"
                     /locus_tag="OO609_003489"
     CDS             6931..8037
                     /gene="nanM"
                     /locus_tag="OO609_003489"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6634773.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8102..9082
                     /locus_tag="OO609_003490"
     CDS             8102..9082
                     /locus_tag="OO609_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6634774.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2790 a         2080 c         1997 g         2665 t
ORIGIN      
        1 cattacccga ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat
       61 accgggatcc cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg
      121 agggatcgtc aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac
      181 cattgtggtt aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc
      241 cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc
      301 actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat
      361 atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc
      421 cacagcctgg ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa
      481 aggtgcctgt gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg
      541 atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga
      601 cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt
      661 ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat
      721 tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat
      781 tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag
      841 gtaatagggt gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt
      901 ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg
      961 gctgataatt gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat
     1021 cgctttaacg ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc
     1081 atcaagaata cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac
     1141 gataaaacga ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt
     1201 actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat
     1261 tacgcgagtc gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat
     1321 gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac
     1381 gtttttatta ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata
     1441 ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc
     1501 acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc
     1561 tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt
     1621 ggaggctatc cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac
     1681 accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat
     1741 aacaaaaggc accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg
     1801 ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat
     1861 gacgccctga aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag
     1921 agcaaacatt ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt
     1981 tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt
     2041 gatactgaac cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg
     2101 cataataacg cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac
     2161 taaatgtcgc accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa
     2221 cgtttgttgc actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat
     2281 caattgccgt acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat
     2341 tcagctgaat gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg
     2401 tacgaacctg tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg
     2461 cgttaacaac ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca
     2521 gagccgctgc ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt
     2581 tcatgctgct ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca
     2641 ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa
     2701 catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt tcagtttatt
     2761 tgttggctta atattctatt attatcttta tttatagatt tttatattgc atgaggtggt
     2821 ttttggagag aagaatgagg aagatgcgtc gagccacaga aacgttagct ttacatatag
     2881 cggaggtgat gtgaaattaa tttacaagag aaataattta catatcaaat agttagatgc
     2941 tttttgtcgt tttttaatat ttttatgctt gagaaaaaat acgtaactta tttatgatat
     3001 ggacagtttg gccccaattg tcttgtgttt atttgttttt taactttatt atcaataagt
     3061 taaatcaaac ctcttctctt tttaattttt cgtttatgag attatttctt tcccataatc
     3121 cggcaaaacg agcagcatta ctggcggtat aacgcacagt atggcgaata tttcgatgcc
     3181 cgagataatc ctgaattaaa cgggtatctg caccacgctc cgccagttca taaccgcaag
     3241 catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata ccggcatcgc
     3301 gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa ataaatatag
     3361 cgtcggtccg gtcagcgcct ttccagttag cacgttcctg ggtccagcgt tccacggctt
     3421 cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat
     3481 ttattcgacc ttcattaagg tcaaggtcct gataatgcag atcaagcagt tcactaatac
     3541 gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc gttgccccgt
     3601 aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga cgtttactca
     3661 caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa acctcaatcg
     3721 gtcaaaatta gcccaataga taaaaagata accacacacg aagttatgtg gctatcagtc
     3781 aattacatta aacatcatac aaaaataaaa tatcactgat gtgacaacat ctattttcat
     3841 tgatttgatt caatataaca atagattaca gaggattaac ttccagttac ttaaaaaata
     3901 aagaaaaaag tgtggaaaca ggagaattta agtgatgtgt aaacttatga atgattttgt
     3961 ttatttattt tgaagaacat aaatcattag attttgtatg ggtatggtta acttaatgtt
     4021 tattatgata attaagaatc acacttacaa taacatcaat taataaatgg ttaatcattt
     4081 ttgatatatc gtaagaataa tgtagttttt aacaccatcc ctggtatctc aactatctct
     4141 ataaaacagc atgacgctgt cgtcctctgg ctctatccca gatgccgtaa aaacgccctg
     4201 cattgctggc ggtataccag acagtatgac gaatattgcg atgcccaaga taatcctaga
     4261 taagtcgcgt atctattccc atattcgcca aagcaaaacc acacgaatag cgtaacatgt
     4321 gcggatgaat ctccagtgac aacccggcat taccaccaga agtcgagata atatggtaaa
     4381 actgttgccg agaaagcgga ttccccttac gtgataaaaa tacccactcg ctctcagcat
     4441 gcggataaga agtacggata ctcaaccagt tttttaaagc ctgaacttct ttgttcaata
     4501 gcgggtgcgt tgttgaaaag ccttttttta atcgatggat atatatacac tttgctttaa
     4561 gatcaatatc cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca tgaataaaac
     4621 aaagcaaaat cagacaataa ttacgtgttg catgaggccc ggtatttgct gctttaagga
     4681 gtgactcgat ttcactatgg gtaaggaagt tccttttttt gttatcagcc ttattcctca
     4741 tcgttttccc ttataattac agacgcgcac tagctgtgct gggttagcaa taatccaaca
     4801 tttttatcct gattatgttt ataaaagcga acgtttgctt aataacttac gtaagtggac
     4861 cagttcttct gagcgaactt aaatggagta gcgatagtta cttataataa ctttgccata
     4921 caaacgccat gctcttgcat gctatgtacc tttatatatt tatcaatctg atacgaatct
     4981 aataataacc ctcatcatta atgcaactaa tcttatctat atatcatgtg atatgttctg
     5041 taacaagtaa aatcaacata aaaaattcag caaaatttaa tatttgttaa actagccgtc
     5101 tcaaatatga cattattagt taactgcatt tgccacaaaa tggctatttc atggaacgca
     5161 gtaatttctt agtggctatt atcatgctaa ttcttagatc cctagcgatt attcctgcct
     5221 ggttcgtcag cttaggccat gagattgtta cattcctttt tatcctggag atatggggga
     5281 acgcaggcga cgctttttca tctacatccg ttggtaaata tagctttggt cactacgact
     5341 taagccttcc tcaatcacag ctattcgccg ggcgatacgg caaacaaact aagtgaaaca
     5401 tcatcctgaa aacaccaaca tcaacaagcc tctccagatc gacatcagga gtgaccagtt
     5461 acaagccaca aaccaagaaa catatttcat gatgagaatt atgctcagcc tgatggcgag
     5521 taaaccctta ttttcaccac tctcactgag ttagcttgca aagctttctt tgtttagatt
     5581 ctaaataaat tatttataaa acgtatagtt aaaagaaaat ctttagtttt tgaagaccac
     5641 cgcaagtgtt cgtctggctt cacatggcat cttcttctta gaaaaagatc gacatatttt
     5701 gtgacgcgaa ttgcaaatct ggttttgttg tatggattgc gtgatttttg atctggtata
     5761 acaggtataa agatgcacca cgatagtcaa tgagactggg catctcgtaa tctatggcaa
     5821 acatcacttc agttctttca catcgggtga tgaaaatgca cttcagtctg aaaggaatat
     5881 gaaaatgaga tcaacagaca ctctatttta tgactctggg taaaatgggt tgagtaagtg
     5941 atataactta cgaatattca aatcaattaa aaatcagaag agattttata ctcaggtatt
     6001 taatctggat ctctgtttat ttaaataatg ttaaaagaga attttcacag gagaccttat
     6061 acaaaaaata taaaatacag ttacccgttg ccaaagacac tataagcctg gcaaaaaaat
     6121 attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca
     6181 aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt
     6241 cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa
     6301 gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa
     6361 gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat gccgcactca
     6421 atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg acggtgcgcc
     6481 cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt
     6541 cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt
     6601 accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat
     6661 atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca
     6721 aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat gcctttgttc
     6781 tacaatacca tatgacgccc gatattacgc catacataga atatgactac cttgaccgcc
     6841 agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat
     6901 ttaaactgta gtagacagga gacagtcaca atgaataaaa ctataatggc gcttgctatc
     6961 atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt gccatttaaa
     7021 agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtaca
     7081 gcatggtaca agctagatac acaggccaaa gataaaaaat ggactgcgtt agctgcattc
     7141 cctggtggac cgagagagca agcaacctct gcatttattg atggcaatct gtatgtgttt
     7201 ggcggcattg gcaaaaacag cgagggattg actcaggtat ttaatgacgt acacaaatac
     7261 aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggt
     7321 catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc
     7381 ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc tatagataaa
     7441 atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg
     7501 tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg
     7561 gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa
     7621 ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg
     7681 aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc
     7741 aatgattctc ttatatttgc tggaggggcc ggattcaaag gttcacgaga aaattaccag
     7801 aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt
     7861 tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta
     7921 tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg cggcaaagcg
     7981 gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca aaactaacgc
     8041 ttcagggccc cggtaaaggg gccctgctat caatttgtca tttgtattac gcgaaaatta
     8101 tatgaacgca ataatatcac ccgattatta ctatgttctt accgttgctg gtcagtctaa
     8161 tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc ctcattccag
     8221 aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat gtcactttaa
     8281 cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg gttatcacca
     8341 tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac tgcatattgc
     8401 acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc cgtgttgccg
     8461 tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc acggagccag
     8521 ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag tcagcagaac
     8581 gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct ggatgcaagg
     8641 cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta atcatatggt
     8701 tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata ttactgacgc
     8761 gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc attcgtatga
     8821 agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg tcgacttcca
     8881 gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg atttaagcac
     8941 gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac tgcgaagcag
     9001 tcatttcagc acggcagccc gtcgggggat tatttctgac agatttgtag aagcaatttt
     9061 gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc ttgttcgttc
     9121 tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact tgcgggctcg
     9181 aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaaaacgc gcaccgcata
     9241 aaccgttgtt attgcttaca gattagccgg atgccttaat agacatccgc gccttttcga
     9301 ttatggctcg gaaatctacg aacatctaca cagtttactg gaacgttttg ggccacagcg
     9361 ttcgcagcac cgtcctgata tgccgttctg gcgattataa gtcgacggat tctggcaaat
     9421 agacgatgat gatatgctta atgatgttca aagaaattag gccattatta tattattgct
     9481 tgtattttct ttcattccta aaagcacccg ccattaatcc caggcgcatt at
//
LOCUS       DAJTYH010000135         9332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTYH010000135 DAJTYH010000000
VERSION     DAJTYH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..138)
                     /locus_tag="OO609_003491"
     CDS             complement(<1..138)
                     /locus_tag="OO609_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6634775.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SA"
     gene            complement(357..1334)
                     /gene="epmA"
                     /locus_tag="OO609_003492"
     CDS             complement(357..1334)
                     /gene="epmA"
                     /locus_tag="OO609_003492"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6634776.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1659..3467
                     /gene="frdA"
                     /locus_tag="OO609_003493"
     CDS             1659..3467
                     /gene="frdA"
                     /locus_tag="OO609_003493"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6634777.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3460..4194
                     /gene="frdB"
                     /locus_tag="OO609_003494"
     CDS             3460..4194
                     /gene="frdB"
                     /locus_tag="OO609_003494"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6634778.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4205..4600
                     /gene="frdC"
                     /locus_tag="OO609_003495"
     CDS             4205..4600
                     /gene="frdC"
                     /locus_tag="OO609_003495"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6634779.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4611..4970
                     /gene="frdD"
                     /locus_tag="OO609_003496"
     CDS             4611..4970
                     /gene="frdD"
                     /locus_tag="OO609_003496"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6634780.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5033..6166
                     /locus_tag="OO609_003497"
     CDS             5033..6166
                     /locus_tag="OO609_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6634781.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6255..6788
                     /gene="blc"
                     /locus_tag="OO609_003498"
     CDS             6255..6788
                     /gene="blc"
                     /locus_tag="OO609_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6634782.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6785..7102)
                     /gene="sugE"
                     /locus_tag="OO609_003499"
     CDS             complement(6785..7102)
                     /gene="sugE"
                     /locus_tag="OO609_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6634783.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7278..7424)
                     /gene="ecnB"
                     /locus_tag="OO609_003500"
     CDS             complement(7278..7424)
                     /gene="ecnB"
                     /locus_tag="OO609_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6634784.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7535..7660)
                     /gene="ecnA"
                     /locus_tag="OO609_003501"
     CDS             complement(7535..7660)
                     /gene="ecnA"
                     /locus_tag="OO609_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6634785.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7712..8278)
                     /gene="efp"
                     /locus_tag="OO609_003502"
     CDS             complement(7712..8278)
                     /gene="efp"
                     /locus_tag="OO609_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6634786.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8320..>9332
                     /gene="epmB"
                     /locus_tag="OO609_003503"
     CDS             8320..>9332
                     /gene="epmB"
                     /locus_tag="OO609_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6634787.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDL"
BASE COUNT         2345 a         2412 c         2401 g         2174 t
ORIGIN      
        1 tgcagaaaat atataaaagg gaatcgcact ataacccatt aagtaataag ccgatgggct
       61 attggcaaat ccaaatacgg aagtaaagat catcaatatg agtcctatca gactcatctt
      121 ttttatcgtg tgaggcatga aaccatcctt catttcagcg cgtgtataac acgtgcccac
      181 tgaactgcac tgcgcaacat tgagcaggca gcgtaaaaac caatacagaa ttgatacttg
      241 gatgatatca aatatccagt cttaaattgt ggcctttaac ggaaaaatga aaacatatgc
      301 tgcagaatgg cgcttatcac gccattcttc gctgttaatt cagtaatttt tcagaattat
      361 gcccggtcaa cgctaaaggc gatgacttca gccagtgtct ccgcgcccag cgccaacatc
      421 accagacgat caacacctaa tgccacgccg gaacagtcag gcataccgac tttcaaggct
      481 tcaatcagat tctggtcaat ggggtgctgc ggcaaaccgc gcgccgcgcg cttacggtta
      541 tcttgttcaa agcgttgttg ctgctcacgg gcatccgtta attcatggaa accattcgcc
      601 agctcaatac ctttataata aacctcaaag cgttcagcga cccgatgatc ttcggtactg
      661 atttgcgcca gtgatgcctg gctggctgga aagtggtaca caaaggtcgg tttttcttta
      721 ccaatatttg gctctacgcc aaaggtaaac agcaattgta gcaacgtgtc gcggtcttct
      781 tcggtatcag caacattgct caaatccagt ttcgctgcga cttcccgcag ttgcgttttg
      841 tcggcagaga gcgggtcaat ttccagataa cgcaagaaag cttgttgata agaaaggctt
      901 tctgctgccg ggcagtccag cacctgttgt aagagatcgt ccacctcgtt catcaaccgg
      961 tacatatcat agtgcggtcg ataccactcc agcatagtga actcagggtt gtgataacgc
     1021 cccatctctt cattacggaa gctgcggcac agctggaata ccggcccaca accagcaacc
     1081 agcaggcgtt tcatatggta ttccgggctg gtcattaacc agagattcat cccctgcgaa
     1141 tgcccggggc caacgaaacg tgtctcaaac gggaccaaat gaatatcggt taccgtcgcc
     1201 tggctcatac aaggcgtctc cacctccagc actccacgat cggcaaagaa acgacggatc
     1261 tccgccataa tcgccgcgcg ttttaataag ttaggaatgg atgcgctcgg ctgccaggat
     1321 gccgtttcgc tcatagttaa atctccagtt tttgacaagg gcacgaagtc tactcgcaac
     1381 gcgacggcga gacaaatttt acgcaggaat caaacagcgg tgggcagtga ctaaaaaaag
     1441 cacgatctga tggtttagta attaaattaa tcatcttcag tgataattta gccctcttgc
     1501 gcactaaaaa aatcgatctc gtcaaatttc agacttatcc atcagactat actgttgtac
     1561 ctataaagga gcagtggaat agcgttcgca gaccgtaact ttcaggcact taccctgaag
     1621 tacggggctg tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt caagccgatc
     1681 ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa
     1741 atccaaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg
     1801 ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc
     1861 acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc
     1921 accactgccc aaccgaaatg acccaactgg aactgtgggg atgcccatgg agccgtcgcc
     1981 cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg
     2041 ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc
     2101 cgcagatcca gcgttttgac gaacatttcg tgctggatat tctggttgat gatggtcatg
     2161 ttcgcggcct ggtagcaata aacatgatgg aaggcacgtt ggtgcagatc cgtgctaacg
     2221 cggtcgttat ggctaccggc ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca
     2281 tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg
     2341 aattcgttca gtatcaccca accggtctgc caggttccgg tatcctgatg accgaaggct
     2401 gccgtggtga aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg
     2461 gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac
     2521 gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc
     2581 cgcgtggtga tgtggtttat ctcgacctgc gtcacctcgg cgagaaaaaa ctgcatgaac
     2641 gtctgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac
     2701 cgattccggt acgtccgact gcacactaca ccatgggcgg tatcgaaacc gatcagaact
     2761 gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg
     2821 gtgcaaaccg tctgggctcc aactccctgg cggaactggt ggtcttcggc cgtctggccg
     2881 gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg gcaattgaag
     2941 cgcaggcagc tggcgttgaa caacgtctga aagatctagt taaccaggat ggcggcgaaa
     3001 actgggcgaa gatccgcgac gaaatgggcc tggcaatgga agaaggttgc ggtatctacc
     3061 gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag gaacgcttca
     3121 agcgcgtgcg catcaccgac acctccagcg tgttcaacac cgacctgctc tacaccattg
     3181 aactgagcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag
     3241 agtcccgcgg cgcacaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact
     3301 ttctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg
     3361 acgtgaagat tactacgctg ccgccagcta aacgcgttta cggtggcgaa gcggatgcag
     3421 ccgataaggc ggaagcagcc aataataagg agaaggcgaa tggctgagat gaaaaacctg
     3481 aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat
     3541 gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac
     3601 ctggcaccgg acctgagtta ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc
     3661 atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac
     3721 ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg
     3781 acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc
     3841 gcggatcagg gtactaacat ccagaccccg gcgcagatgg caaagtatca ccagttctcc
     3901 ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag
     3961 ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac
     4021 ggtaagaagg agcgtatggc gcagttgaac agccagaacg gtgtatggag ctgtactttc
     4081 gtgggctatt gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag
     4141 ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg
     4201 caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa
     4261 attgccgttt tatcgctttt acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt
     4321 cagcattgaa ctgattttcg cgctgtttgc cctgaaaaat ggcccggaag cctgggcggg
     4381 attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca ctctgacggc
     4441 agctctgctg cacaccaaaa cctggtttga actggcaccg aaagcggcca atatcattgt
     4501 aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt
     4561 tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc
     4621 caaatccaaa gcgttctgac gaaccggtat tctggggcct cttcggggcc ggtggtatgt
     4681 ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg tattctgctg ccactggggc
     4741 tgtttccggg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg
     4801 gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgtatgc
     4861 accacgcgat gcacgatctg aaaatccacg tgcctgcggg caaatgggtt ttctacggtc
     4921 tggctgctat cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa cgcatcgcca
     4981 atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa
     5041 aacgacgctc tgcgccttat taattaccgc ctcttactcc acatttgctg cccctcaaca
     5101 aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg
     5161 tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga
     5221 catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa
     5281 aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga
     5341 tcccgcaaca aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt
     5401 acatcttgcg acctacaccg ctggcggcct gccattgcag gtgccggatg aagtgaaatc
     5461 ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca
     5521 acgtctgtat gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg
     5581 tttgagtttt gagcaggcga tgcaaactcg tgtcttccag ccactcaaac tcaaccatac
     5641 gtggattaat gtacctcccc cagaagaaaa gaattacgcc tggggatatc gcgaaggtaa
     5701 ggcagtgcat gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat
     5761 tgaagatatg gcccgctggg tgcaaagcaa tttacccccc cttgatatca atgagaaaac
     5821 gcttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca
     5881 gggcctgggc tgggaaatgc tggactggcc ggtcaatcct gacagcatca ttaacggcag
     5941 tgacaataaa attgcactgg cagcacgccc ggtaaaagcg attaagcccc caactcctgc
     6001 agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc
     6061 gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc
     6121 agcgagagtc gccgccgcct gtcagattct caacgctcta cagtaaaatt tcatcgggtc
     6181 cgaattttcg gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca
     6241 gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg
     6301 tcgttgcctg tagttttcct acgccgccgc gtggcgtgac cgtagtgaat aatttcgacg
     6361 ccaaacgtta tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg
     6421 gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg aatgtcatta
     6481 ataaaggcta taaccctgac agaggaatgt ggcagcagag tgaagggaaa gcgtacttta
     6541 ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt
     6601 ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg
     6661 actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc
     6721 tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg
     6781 gtagttagtg agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg
     6841 ccagacgcat cgggttagcg gactcaccga gcagcacaat gccggtgatg gccgcgccga
     6901 ctgcgccaat acccgtccac acggcataag ccgtccctac cggtaacgat ttcatcgccc
     6961 aggcaagtag cgccatactg actatcatcg ccgtcacggt aataacactc ggcgtcaaac
     7021 ggctaaagcc gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa
     7081 taactaagat aatccaggac atatcaggct ccggaaattt ggggccgtcc ccggtgaaaa
     7141 gaggcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca
     7201 ggggaatttc aactattacc tttatgacga tcgcagaaaa aacaaacggc acgacactgc
     7261 tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
     7321 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
     7381 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
     7441 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgat
     7501 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga gagatggagt
     7561 taccgagatg tttgatgtct tcgccgaaac cgcaagcggt gttacagccc gtgagcagcg
     7621 tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg aataagtgat
     7681 ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag
     7741 agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaact ttaaccacag
     7801 cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg
     7861 gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag atcggctgac
     7921 cattccacag agttacgata cactctgcct gatccagcag ccatttagca ttgtcgccaa
     7981 ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag tgccagaact
     8041 caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct tcagcggaat
     8101 cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc agtttaacgc
     8161 gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc gcgtaaggtt
     8221 cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac gttgccataa
     8281 ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat tgtaacccta
     8341 aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac cgatcctgat
     8401 gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc
     8461 aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat
     8521 ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt cgtcgcgtcc
     8581 ggattctcca ccgacccact ggaagaacag cacagcgtag tgcctggttt gttgcataaa
     8641 taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc
     8701 ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg gcagactgca
     8761 cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg cggcgatccg
     8821 ctgatggcga aagagcacga gctggactgg ttgctcacac aactggaagc catcccgcat
     8881 atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg tatcaccgag
     8941 gcgctggttg aacgctttgc ccgttctacg ctgcaaatct tgctggtgaa tcacatcaat
     9001 catgccaatg aggtagatga aacgttccgt caggcgatgg ctaaattgcg ccgtgtcggt
     9061 gtcaccctgc ttaaccagag cgttctgtta cgtggtgtga acgataacgc acaaacgttg
     9121 gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct gcatgtgcta
     9181 gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg gcagattatg
     9241 cgtgagttgc tgacattggt gtcgggatat ctggtgccga aactggcgcg agaaattggc
     9301 ggcgaaccca gcaaaacgcc gctggatctc ca
//
LOCUS       DAJTYH010000136         9293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTYH010000136 DAJTYH010000000
VERSION     DAJTYH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..258
                     /gene="mliC"
                     /locus_tag="OO609_003504"
     CDS             <1..258
                     /gene="mliC"
                     /locus_tag="OO609_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6634788.1"
                     /translation="VERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGL
                     SASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            317..973
                     /gene="pdxH"
                     /locus_tag="OO609_003505"
     CDS             317..973
                     /gene="pdxH"
                     /locus_tag="OO609_003505"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6634789.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1102..2376
                     /gene="tyrS"
                     /locus_tag="OO609_003506"
     CDS             1102..2376
                     /gene="tyrS"
                     /locus_tag="OO609_003506"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6634790.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2435..3298
                     /gene="pdxY"
                     /locus_tag="OO609_003507"
     CDS             2435..3298
                     /gene="pdxY"
                     /locus_tag="OO609_003507"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6634791.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3342..3947)
                     /gene="gstA"
                     /locus_tag="OO609_003508"
     CDS             complement(3342..3947)
                     /gene="gstA"
                     /locus_tag="OO609_003508"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6634792.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4053..5555)
                     /gene="dtpA"
                     /locus_tag="OO609_003509"
     CDS             complement(4053..5555)
                     /gene="dtpA"
                     /locus_tag="OO609_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6634793.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6166..6801)
                     /gene="nth"
                     /locus_tag="OO609_003510"
     CDS             complement(6166..6801)
                     /gene="nth"
                     /locus_tag="OO609_003510"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6634794.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6801..7496)
                     /gene="rsxE"
                     /locus_tag="OO609_003511"
     CDS             complement(6801..7496)
                     /gene="rsxE"
                     /locus_tag="OO609_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6634795.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7500..8120)
                     /gene="rsxG"
                     /locus_tag="OO609_003512"
     CDS             complement(7500..8120)
                     /gene="rsxG"
                     /locus_tag="OO609_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6634796.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8124..9182)
                     /gene="rsxD"
                     /locus_tag="OO609_003513"
     CDS             complement(8124..9182)
                     /gene="rsxD"
                     /locus_tag="OO609_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6634797.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2326 c         2358 g         2225 t
ORIGIN      
        1 gttgagcgaa tgcaaaccga tacgctggaa taccagtgtg atgaaaaacc gttgacggtc
       61 aaactgaata atccgcgcca ggaagtcagt tttgtttacg ataatcaact actgcatctc
      121 aaacagggcc tttcagcctc tggcgcgcgt tactccgacg ggatctatgt tttctggtcg
      181 aaaggtgagg aagcgactgt ctataaacgc gaccgcatcg tcttgaataa ctgtcagtta
      241 caaaatccac agcgttgaga tttttccagg ggcggcgcac aatagcgtca cccactgaca
      301 atccgtaaag aaaaccatgt ctgataacga cgaattgcag caaatcgcgc atctgcgccg
      361 tgaatacacc aaaggcgggt tacgccgccg cgatcttccc gccgatccat taaccctttt
      421 tgaacgttgg ctctctcagg cttgtgaagc caaactggcg gaccctaccg cgatggtggt
      481 cgctaccgtg gatgaacatg gtcagcctta tcagcgcatc gttttactca aacattacga
      541 cgaaaaaggc atggtgtttt acaccaacct cggtagccgt aaagcacatc aaattgaaaa
      601 taatccgcgc gttagcctgc tgttcccgtg gcataccctt gaacgccagg tgatggtgat
      661 cggtaaagca gaacgacttt cgactctcga agtgatgaaa tattttcata gccgcccgcg
      721 tgatagccag attggtgcat gggtttcgaa gcagtccagt cgcatttctg cccgcggtat
      781 ccttgaaagt aaattcctgg agctgaagca gaagtttcaa cagggcgaag tgccattgcc
      841 gagcttttgg ggcggttttc gcgtcagcct tgaacagatt gagttctggc agggcggtga
      901 gcatcgcctg catgaccgct ttttgtacca gcgtgaaaat gatgcgtgga agattgatcg
      961 tcttgcaccc tgaaaagatg caaaaatctt gctttaatcg ctggtactcc tgattctggc
     1021 actttattct atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc
     1081 gttatataca tggagatttt gatggcaagc agtaacttga ttaaacaatt gcaagagcgg
     1141 gggctggtag cccaggtgac ggacgaggaa gcgttagcag agcgactggc gcaaggcccg
     1201 atcgcgctct attgcggctt cgatcctacc gctgacagct tgcatttggg gcatcttgtt
     1261 ccattgttat gcctgaaacg cttccagcag gcgggccaca agccggttgc gctggtaggc
     1321 ggcgcgacgg gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc
     1381 gaagaaactg ttcaggagtg ggtggacaaa atccgtaagc aggttgcccc gttcctcgat
     1441 ttcgactgtg gagaaaactc tgctatcgcg gcgaacaact atgactggtt cggcaatatg
     1501 aatgtgctga ccttcctgcg cgatattggc aaacacttct ccgttaacca gatgatcaac
     1561 aaagaagcgg ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt cactgagttt
     1621 tcctacaacc tgttgcaggg ttatgacttc gcctgtctga acaaacagta cgatgtggtg
     1681 ctgcaaattg gtggttctga ccagtggggt aacatcactt ctggtatcga cctgacccgt
     1741 cgtctgcatc agaatcaggt gtttggcctg accgttccgc tgatcaccaa agcagacggc
     1801 accaaatttg gtaaaactga aggcggcgca gtctggttgg atccgaagaa aaccagcccg
     1861 tacaaattct accagttctg gatcaacact gcggatgccg acgtttaccg cttcctgaag
     1921 ttcttcacct ttatgagcat tgaagagatc aacgccctag aagaagaaga taaaaacagc
     1981 ggtaaagcac cgcgcgccca gtatgtactg gcggagcagg tgactcgtct ggttcacggt
     2041 gaagaaggtt tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt
     2101 gcgctgagtg aagcggactt cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg
     2161 gaaaagggcg cagacctgat gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt
     2221 caggcgcgta aaactatcgc ctccaatgcc atcaccatta acggtgaaaa acagtccgat
     2281 cctgaatact tctttaaaga agaagatcgt ctgtttggtc gttttacctt actgcgtcgc
     2341 ggcaaaaaga attactgtct gatttgctgg aaataatgca ttaagtggaa agggggagtg
     2401 agaaatcact ccccctggtt tttatacagg gaacatgatg aaaaatattc tcgctatcca
     2461 gtctcacgtt gtttatggtc atgcgggtaa cagtgcggca gagtttccga tgcgccgcct
     2521 gggcgcgaac gtctggccgc tgaacaccgt tcaattttct aatcacaccc aatacggcaa
     2581 atggactggc tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag gcattgccgc
     2641 cattgataaa ttacacacct gtgatgccgt attaagtggc tatctgggat cggcggagca
     2701 gggtgaacat atcctcggta tcgtccgtca ggtgaaagca gcgaatccgc aggcgaaata
     2761 tttttgcgat ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc
     2821 agagtttcat gtgcggcacg gtttgcctgc cagcgatatc attgcgccga atctggttga
     2881 gctggaaata ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg
     2941 cgaactcatt gagcaagggc cacaaattgt gttggttaaa cacctggcgc gagctggcta
     3001 cagccgtgac cgttttgaaa tgctgctggt caccgccgat gaagtctggc atatcagccg
     3061 tccactggtg gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt
     3121 actgctggtg aaactgcttc agggggcaac gctgcaggag gccctggaac atgtgaccgc
     3181 tgcagtctac gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt
     3241 ggctgctcag gatcgtattg ccaaaccaga acattacttc agcgcaacaa agctctgaaa
     3301 tatctcattt cggcccacaa cgacctgtgg gccgatggct attactttaa gccttccgct
     3361 gacagcgcgt cttgtacttc cggacgttca gccatacgtt gcataaatgc cgcaatgtgc
     3421 tctaaccctt ccagattcag tttcaccgcg tatgcccagc gcagaaccgt aaacagatag
     3481 gcatcagcaa ttgtaaatct ttgcccgcag atccagtgct catccttcag tgcctcgttc
     3541 acatattgca gcttcttatc cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta
     3601 tctgggcgaa acagaggtgt gaaaccttta tgcagctcgg tggcgatgta attcagccat
     3661 tcgatggttt tatagcggga aatactgttt accggtgcca gcaactggcg gtcggggacg
     3721 ctgtcggcaa gatactgcat aatcgcaacg ccttccgtca gcaaagtacc atcgtccagc
     3781 agcaatgcag gcacctgccc cttagggtta acggaaaagt aatcgtcacc gttttcgaga
     3841 cgttttttca ttaaatccac actgacgagc gtaaaatcct ttccgctctc acgcagggtg
     3901 atatgggaag cgagagagca ggcgcccggt ttgtagaaca atttcatcgg taactccttt
     3961 tgtctgtggt ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa
     4021 gttagcggct tgtaaaagag tttccctgaa ttttacgcta cagtagcttt cgccgctttg
     4081 tctgcagcgt catcctgcgt catgcggtgc agtttaggcg cggtcagcag catcagtact
     4141 gcaataacgg cagtagcgac accaatctgc aagaatacgc gaccatagac ttccagtgac
     4201 atcagcgggt cggtaacgtt atccggcaca gccatcatac ccgcaacata accaccaatc
     4261 aggtttgcac cggcagtggt caggaaccag ctacccataa tgaagcccat cagacgctgc
     4321 ggaacgagtt gtgcaaccat tgccagaccc agaccagaga tcatcagttc cccgatgctc
     4381 tgcaggccat agcttgcgac cagccagctg acagacacga tacctgcgtc agacgcgaat
     4441 ttcgcaccca gcggcagaat caggaacgca ccagaacaca tcaccatgcc gattgcaaac
     4501 ttggttggca tcggcagggt atcacccatc ttgttataga tagcggccag aatcggacta
     4561 ccgatgatga tccagaacgg gttcagtgcc tgatactgct caggttctac ggccagaccc
     4621 agaatggagt gctcaacgtt acgaatcgca aagaagttca gtgacgttgg catctggctg
     4681 tacagcacga agaagatgat tgcttcgagc atcaggatga aggcaacgat cattttacga
     4741 cgcgcagcac ctttcatcgc gaaagcttct ttaccgaaga taaccacaat accgaaggca
     4801 acaacgccca gcgccatacg cgcaacttcc tgattgtgca gtagccaggt ggcaacagcg
     4861 atcagcgcca caacaccaat aatggtcagc aacaggttac ggtagttgac tggttcaaag
     4921 tctggttttg aaccgtactg tttaacccag cgttggcaga aggcgaagtt aacgatagtg
     4981 atcagcaggc ctacaacgct caacgcaaac gcaacactcc agccgtattt cgcggccagc
     5041 cacggggtgg ctatcataga gaagaaagag ccgatgttga cggacatgta gtacatggtg
     5101 aatgcaccgt ccagacgtgg gtcgtttttc tcatagcatg tagaaagcag agaagacggg
     5161 ttagctttaa acaggccgtt accgaccgca atagccgcca tacccatata aacaataccg
     5221 gcgtcgtgac cagaccaggc aaccagagca taaccaatcg ccagcacaat agcgccgagc
     5281 ataattacgc gtttagtacc cagtaccttg tcacctaacc agccgccgat agcgaccaga
     5341 ccataaacca gggcactaaa ggaagagaaa agggtgattg aatccgcttc agacataccc
     5401 agttgtttaa ccaggtaaac agccataatt ccttgtaggc cgtaataacc aaaacgttcc
     5461 cataactcaa tcgagaagat gagatagaac gccttcggtt gtttgaaagc gttcaaactg
     5521 acgctttcag ttggtttttg gtttgcagtg gacactttta cctctttttt tacatcccat
     5581 attaacgggg gtgctttctc gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg
     5641 gaggggaaac ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa
     5701 tctgttacat ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta
     5761 aatttttcaa aaaacgtaac ggcagatttt ttcactgcat tgagaggggg atgatggctt
     5821 ggcggcgata tgttctgcta tttagcgttt ttcctagtga gatagtccag tttctgaaaa
     5881 atagccagtg taatgttttg caggtcaata tttgttgcgt gtgtaaattt aaatcatttt
     5941 aacctgattc ttgcaatgtt tttacattgc cacagcatcg tgatcttgat cacgaatgaa
     6001 tagaaaattt cgctattaaa aaaacaatta acctgcattt ttggttatcc gcaaacggat
     6061 tacctgatga taacgctaag caatggcatc attgacgtta aaaagatgtt ttaaaagagg
     6121 ataaagaaag gttatcaatg gggtaatcgg tgttacccct tttcttcaga tgtcaacttt
     6181 ctctttgtat tcacaaagat cttcaataat acaagagcca cagcggggct tgcgggcaat
     6241 gcaggtataa cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg
     6301 aaccactttc agtagctttt cttctacctg ttcgacgttt ttccccggcg caaattgagt
     6361 acgattacaa acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc cgaatgcagt
     6421 gtttaatacg acgttggctg ttttacgacc tacgccgggc agggcttcaa gcgcagcacg
     6481 atcttccgga acctcgccat tatgttgttc cagtaatata cggcaggttt tgatgatatt
     6541 ttctgctttg ctgttataaa ggccaatcgt tttgatatag gttttcaccc cttcaacgcc
     6601 tagttcaagc atcgctgcag gcgtattcgc caccgggtag agtttcgccg tcgccttatt
     6661 aacactgata tcggtcgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga
     6721 actgaaatta agctcggtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc
     6781 cagacgtttt gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg
     6841 gcagctgctt cagcacggcg ctttttcatt ctttcatcaa tcaggtattt tcctgccagc
     6901 atcagtccca agccaataaa tgcacctggt ggcagcatcg ccagcaggaa aggggagtcg
     6961 gtgtggaaaa tctccacgcg taatactttt gcccagctac ctaacagcgc atctgcaccg
     7021 tcaaacaacg tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag
     7081 gttgcgcccg taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg
     7141 gcgaaggctt cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc
     7201 cctaatgatt gatacaggcc aaaggcgtag gcgttgatca gcatctgtac agcgctgacc
     7261 accgaggcga tgatcatcac gtaaatggga atgcggatct cggctggcgt ccagtgacgc
     7321 agcgtcgaaa tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc
     7381 agagcgttag tggcagtgga tgtgaccgcc aatagcggac aaaggccgag cagttggacc
     7441 agtgcagagt tgtttttcca caacccctga acaataacgt ctttaatttc gctcacgctt
     7501 tattctccac aggcaggaag ttgagaaagt tgtgccggta acgtctgagc gtacaatccg
     7561 gcgcgtttta ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg
     7621 aaatcaccac catctttctt caccgcccag tgcgcatcat ccgcaccact gatttttttt
     7681 cccgcaaaat gggtgagcca gtcagaaagg cgcaattcga ttttatcgcc aagccctggc
     7741 gtttcgtggt gctctgtcac gcgcgtgcca agtaccgtgc cattaaaatc ggctcccacc
     7801 agcagctgaa tcgcaccgga atagccatct ggcgcggttg cttccagaac ggcggctacc
     7861 ggtttgtcat cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca
     7921 gttaccagat agcaactctg tgcgagcgca ttgttatagc gttcggctgg cagcacctga
     7981 tcaaataacg tcttttgttg cagactggcc tgttcagcaa ttgtcgtttt ggtcatctgg
     8041 ttgatggccg cagttaaccc tgtagaaccc gctgcaaata gtgccagtgt aatgccgtgt
     8101 tttcggatag ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg
     8161 tagtaatcga tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca
     8221 ggatagccgc cgaaactgcg gatcaaccag actaataagc ccgccagcgc gccgaacatc
     8281 agccgaccac gattggtcgt agaagcagta actgggtcag tcaaaataaa gaatgcgccg
     8341 agcatagtcg caccagacag cagatgaatt tgcggtgctg ccagtgtttc tggtgagaac
     8401 aaccagccca acgttgcgca taacgccagc gttactaaga agctgagggg aatatgccag
     8461 cgaatcgctt tctgccatag caaccacacg ccaccagcca gccaggcgag atttacccat
     8521 tgccaaccag cgcccgccag aataccgctg tagatcggat attgcataat ctgttcaacc
     8581 gaatgacctg cgcggacaga ggttttgaat gtatccagcg gtgtcgcctg actaatgcca
     8641 tcaataccta agcgtagcgt attcatatca ccaccactgg cggtatgacc gctgaaaata
     8701 acctgtatgg cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag
     8761 ctggtcatct gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta
     8821 aacgggtttt gccccagacc gccatacaac tgtttagcga tgatcaccgc aaacaccgta
     8881 cccagtacga ccatccacca tggcgctagg gggggaatac ttaccgccag caataagcct
     8941 gtcagcaatg ctgagttatc tttcaacgtt gcggctaccg actgcttgcg tagtttgagt
     9001 acgagagctt cggctaacag agcactgacc gacgccaaca ggatctgaac gagagtaccc
     9061 caaccaaaaa accgcagttg cgctgcgatt cctggcacgg ctgcgagcaa caccaacagc
     9121 ataatgcgcg atgtctggcg ctggttatgg gtataagggg agctagctat tctgaatacc
     9181 atttagtcct cgtttacaac cttttgctgg gcggcttttt tggcctgaac gcgggcaata
     9241 gccgcggcaa ctgcggcttt gcgcggatcg acctgttctt ctggttccgc att
//
LOCUS       DAJTYH010000137         9286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTYH010000137 DAJTYH010000000
VERSION     DAJTYH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            508..1203
                     /gene="minC"
                     /locus_tag="OO609_003514"
     CDS             508..1203
                     /gene="minC"
                     /locus_tag="OO609_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6634798.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1227..2039
                     /gene="minD"
                     /locus_tag="OO609_003515"
     CDS             1227..2039
                     /gene="minD"
                     /locus_tag="OO609_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6634799.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2043..2309
                     /gene="minE"
                     /locus_tag="OO609_003516"
     CDS             2043..2309
                     /gene="minE"
                     /locus_tag="OO609_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6634800.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2681..3025)
                     /locus_tag="OO609_003517"
                     /pseudo
     CDS             complement(2681..3025)
                     /locus_tag="OO609_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3060..3179)
                     /locus_tag="OO609_003518"
     CDS             complement(3060..3179)
                     /locus_tag="OO609_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6634801.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3140..3325)
                     /locus_tag="OO609_003519"
     CDS             complement(3140..3325)
                     /locus_tag="OO609_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634802.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3426..3599
                     /locus_tag="OO609_003520"
     CDS             3426..3599
                     /locus_tag="OO609_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634803.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3601..3945
                     /locus_tag="OO609_003521"
     CDS             3601..3945
                     /locus_tag="OO609_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6634804.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3949..4284
                     /locus_tag="OO609_003522"
     CDS             3949..4284
                     /locus_tag="OO609_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6634805.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4341..6662)
                     /locus_tag="OO609_003523"
     CDS             complement(4341..6662)
                     /locus_tag="OO609_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6634806.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6779..6988)
                     /locus_tag="OO609_003524"
     CDS             complement(6779..6988)
                     /locus_tag="OO609_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634807.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7739..9262)
                     /locus_tag="OO609_003525"
     CDS             complement(7739..9262)
                     /locus_tag="OO609_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6634808.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIVLKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2597 a         2150 c         2040 g         2499 t
ORIGIN      
        1 taatccaacc gtacaacact tatgctcatt tcgtcatcaa tccgtaatca aaaccgctga
       61 cctggacgag ttacgatgac ctgacagaag tatcagacgt cttctgcggt ttctatgata
      121 gagcaattct gtggcggata ttaagataac gggtatctgt tctttaatga tgctgcaaaa
      181 aatggaaagt taagaaagcc tggcgtggtg aaaaaataca cttgcctcaa tactggatat
      241 ttaaacaggt ctggagatgc tgtcacgatt agggtgcgga aaaaggaagc gtaaataaga
      301 agaatcaaaa gtacgacggc aatgggttga ttgacaaggg tattttttaa gctatgaatc
      361 agcgccattt atcacagaat agacttttac tctgaataaa tgggagggtg acttgcctca
      421 atataatcca gactataaca tgccttatag tcttcggaac atcatcgcgc gctggcgatg
      481 attaatagct aattgagtaa ggccaggatg tcaaacactc caatcgagct taaaggcagt
      541 agcttcactt tatctgtggt tcatctgcat gaggcagaac ctaaggttat ccatcaggcg
      601 ctgaaagaca aaatcgctca ggcccccaca tttttaaaac atgcccccgt tgtactcaac
      661 gtcagtgcac tggaagaccc ggtaaactgg tcagcgatgc ataaggcggt ttcggcaacc
      721 ggtttgcggg ttattggcgt aagtggctgc aaagatgcgc aacttaaagc cgaaattgaa
      781 aaaatggggc tgcctatcct gacggaagga aaagaaaaag cgccacgtcc agctcccgca
      841 ccgcaggctc cagcgcaaaa tacaatgccg gtcacaaaaa cgcgtttaat agataccccg
      901 gtgcgttccg gtcagcgtat ttatgctcca caatgtgatc tgattgttac aagccacgtt
      961 agcgctgggg ccgaattgat tgccgatggg aacattcatg tctatggcat gatgcgcggt
     1021 cgtgcgctgg caggggcaag tggtgaccgg gaaacgcaaa tattttgtac gaacctgatg
     1081 gcggaattgg tgtccatcgc aggtgaatac tggctgagtg atcaaatccc agcagaattt
     1141 tatggcaaag cggcgcgact gcagttagtc gaaaacgctt tgaccgttca accgttaaat
     1201 tgatcccttt ttaacaagga atttctatgg cacgcattat tgttgttact tcgggtaaag
     1261 ggggtgttgg taagacaacc tccagcgcgg ccatcgccac tggtttggcc cagaagggaa
     1321 agaaaactgt cgtgatagat tttgatatcg gcctgcgtaa tctcgacctg attatgggtt
     1381 gcgaacgccg ggtcgtttac gatttcgtca acgtcattca gggcgatgca acgctaaatc
     1441 aggcgttaat taaagataag cgtactgaaa atctctatat tcttccggca tcgcaaacac
     1501 gcgataaaga tgccctcacc cgtgaagggg tcgccaaagt tcttgatgat ctgaaagcga
     1561 tggattttga atttatcgtt tgtgactccc cggcagggat tgaaaccggt gcgttaatgg
     1621 cactctattt tgcagacgaa gccattatta ccaccaaccc ggaagtctcc tcagtacgcg
     1681 actctgaccg tattttaggc attctggcgt caaaatcacg ccgcgcagaa aatggcgaag
     1741 agcctattaa agagcacctg ctgttaacgc gctataaccc aggccgcgta agcagaggtg
     1801 acatgctgag catggaagat gtgctggaga tcctgcgcat caaactcgtc ggcgtgatcc
     1861 cagaggatca atcagtattg cgcgcctcta accagggtga accggtcatt ctcgacatta
     1921 acgccgatgc gggtaaagcc tacgcagata ccgtagaacg tctgttggga gaagaacgtc
     1981 ctttccgctt cattgaagaa gagaagaaag gcttcctcaa acgcttgttc ggaggataag
     2041 ttatggcatt actcgatttc tttctctcgc ggaagaagaa cacagccaac attgcaaaag
     2101 aacggctgca gattattgtt gctgaacgcc gtcgcagcga tgcagaaccg cattatctgc
     2161 cgcagttgcg taaagatatt cttgaggtca tttgtaaata cgtacaaatt gatcctgaga
     2221 tggtaaccgt acagcttgag caaaaagatg gcgatatttc tattcttgag ctgaacgtga
     2281 ccttaccgga agcagaagag ctgaaataag cccgctgtaa aaacgcattt atcttcaagg
     2341 cagagtcatt tctgccttga gtttttcatc cctctcatcc acgttgtggt aaagcggcga
     2401 gtattcttgc tgatactcct cattgctatt tccacctccc cctttacacc ttaaggctgt
     2461 tcccacgcac agaacaaaat gtttatgcct ggttgagtaa ataaccatgt tattagtgtg
     2521 tatatactgg gagatgataa ccccgcaatt tcattattca ttgttctccc ataggatgag
     2581 gtttcccgcc gtaactggcg ggcttttttt gccccagaat tttctctttc aacatcctgt
     2641 aaacgaaaac tgcgccgaag cgcagtttaa tcgttgatgg tcagaacgac cagttcacac
     2701 cagccaccgc gttccacggg gactccacac cggcaccatg gctatacccc accccgaaat
     2761 gcccgcttaa cgtactgctg aatgaggctt taatacctgc ctggtatatt ccacgtctgc
     2821 ccgacaaatc attgacaaaa ttaccgtcac tattcacttt cacccgatta tcatcgacaa
     2881 attctttgcg cacagccgcc ttcaggcagg gttcaatttc cataccgttc cccagacgca
     2941 tgttgtaact cagcgttgca cccagttcac gatatatact gcgggtatcg actgatttcg
     3001 attccatgcc attggataaa tgatagtagc gtctaagtga cctaagctct gctgcaacat
     3061 cagcccgcag gcaccagacc aggggattca tcctgaagag acagcgcagg tgtattgtgt
     3121 tcaccgctca ccaaagacat catgatgaaa tgatgatatc ccgcataaga aagaggcatt
     3181 ttttacacgc agtgcgctga agtgtagttg gataaaaaat cactcttttc agataatact
     3241 gtcgtacgct tatctttaga caatgaggcc gtcggaaaaa taaaatgact aatgtaatcg
     3301 ttgctgatga atatcttctg ctcacataaa aatcacacaa taatattgat aaatcagatt
     3361 atcttacctt tacaacattg cgttaacagt caatgacact tcttgaataa aaatattgtc
     3421 aatcaatgag ttattcctca tttaagatta ttctcattca cgtaaaaaac ataattccta
     3481 tcatcacagc cacgctcatt ttgaattatc tgaataatag cgagcgttct ttagttaagc
     3541 agatcttaat tgaagacgaa attattgtgt gtgcaactta cttaataccg gacatctaag
     3601 ttgaagaaaa aaatacttgc cttcgggctt atctcagcat tgttctgctc cacgccagcg
     3661 atagcggata tgaaccgcac cacgaaaggt gcattgctag gcgcaggcgt tggtttactt
     3721 actggcaatg gtgttaatgg cgtactgaaa ggtgcagctg ttggcgctgg tgttagtgca
     3781 gtaacagaaa aaggccgcga cggtaaaaat gcgcgtaaag gtgcaaaagt gggtgccgcc
     3841 gttggtgctg tcactggcgt tctgaccggc aatggcctcg aaggggcgat taaaggtgcc
     3901 gtgattggcg gtacgggtgg tgcaattctg ggtaaaatga aataaggaat gatgatgaaa
     3961 aaatttgctt tactggcggg cctgtttgtt ttcgcaccaa tgacctgggc gcaggactac
     4021 aatattaaaa acggtttgcc gtcagaaacc tatattactt gcgcagaagc caacgaaatg
     4081 gcaaaaactg acagcgcaca ggttgcagaa attgttgcgg taatgggtaa tgccagcgtt
     4141 gccagccgtg atttaaaaat tgagcaatct ccggaactga gcgccaaagt ggtagaaaaa
     4201 ctgaatcagg tttgcgctaa agacccgcaa atgctgttga tcacggcaat tgatgacacc
     4261 atgcgtgcta tcggcaaaaa ataatatttc atatgtaccg cgtcgatgaa tacgacgcgg
     4321 ttttttagct aacggcggga tcagaactta tatttcaggc ccaccatcac tgcggtgtca
     4381 ttgtagccat tatcacccag ctgcacgccc acattccccc agacgctggc cgctggattc
     4441 aggttgccat ttacgccaag tttaatttcc cccaaattac tcaccccatc ttgttcgact
     4501 ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatccagtt catttcaaca
     4561 aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg aataaaggtg
     4621 cggaagccga gtcgggtctg gatgttgtta tttgcgtcat tctcaatacg tgagccattg
     4681 gcttccgtgt gcgcattatg atcgacgccc atccagatag cctgtgcctg tggctggaag
     4741 atccacgtat atttcgccgc ttccagacca aaggatttat tcagggcaaa accgtaaccc
     4801 gcttccagcg agacggttgc acctttcgcg ctataggatt cataggctaa ttcatcccct
     4861 ttcacggagt ttttaaacca gctatattgc gcccaggagt caatgtatgc gcctttttta
     4921 ctgatgtcat cggcaaacca agtggcatac agccctgcgc tatagcctct gacactccct
     4981 ttcgaacgat aatccgacac gctggaatga gttaagttgt agtcgcgggc ataaccagcc
     5041 atcactccca aacgccaact gtcactatcg gtaaaaccac cggttaacag atcgccccct
     5101 aactgcgaga cgtagcgatg cgaggtcgtt ctcaactgtc cgttgctgtc acgccaggca
     5161 ttatgcccgc caatttgacg tagccaaagt cggcttgaac gctcctgttc agttacagga
     5221 tcgatgtagc gcgtttcacc cgcacggtcg ttcagacgca taacaaaaag agtgttagcg
     5281 gctgccaggt tggcgacgta acttccagct tcggggcgca ggacgggggt aggcgcaaca
     5341 gtaggcgctg aacttcctcc ggagtttgtt tcaccgccgc tcgttgcctg gaagttagtc
     5401 agataccagt ttttattggt cgcttcggta ttacctcggg ttaatgaata ttcgtaggca
     5461 ccggcgaaaa tcctcgaatc cttaataaat tccccatttg attccccctc aacgctgata
     5521 atctctatcc catttaacgt gtaagctccg ctgcctcctg cattggtaac tttaactcgg
     5581 gttgttccag aggtatttcc attaataacg actttatccg tagctgaatt atcatcatcc
     5641 aattcagttc tgagatgcaa tacgccatta ttaccaacgt aattttcagt tattgttaat
     5701 cttgttggtt cgacatcccg tccatctgcc cgggaaatat agaccgtact attatcaacc
     5761 gtgaggttgc tcaccgttga gtcagcttta atatcccagg tactattatc tgaaagcgac
     5821 agataggtat gagtgttatc atctgttgaa atattagctg aacccgttat ttttgaatta
     5881 gttgcatcga cggtcaaatg acctttaata ttattgataa aaacatctgc agccgatgat
     5941 acctcactat ccgtaatggt cacgttactc gtctcatctt tatggtagag gatttcattt
     6001 gctgaccgta gtgagctacc agataaaata tctaccgttg cttcctgacc atttatggaa
     6061 gactccagca caattccaga cccctgatgc gcgactgcag atgtattttt cagcgtcacc
     6121 gtgccgccat tataaatttt agcaatcgcg gcgtcaggac ccgttgtata aatttctccc
     6181 tggttaaatg aaactgtccc ctggcgggca gcgatggcat gagcattgtc cccatgcgtg
     6241 gtgatatcaa catcggttaa tgtaacgctg gcattggtat aatcaagcgt gtcaccagcc
     6301 ttgacgacag tgccattagc gtacgcggca taactttctg ttccgtgggt tgtgatctcc
     6361 ccttttgcca tatccagttt tgctatggca ttattttcat tacttcctgt atcggcagta
     6421 ctttcttcat caagagaaga tacacacgca cacgcgcttc cgggagatgc acacgcaaga
     6481 acgccgactg cgtgattacc cgcagtaaca ataaatgtat catctccgtc agaaaaagag
     6541 ggggtaaccg aaccgtcaga gttgactctt attatggtgt cagtattcag gtttccactc
     6601 gtttgactta gcaaaccaat tgagtaatcc cccgttgtga caatcgtgtt tttaccctgc
     6661 aaaataacag caccgccaaa ttcacttacg attgcgctag ctgttaatgc tgggcttaga
     6721 ggactattgt cagaaggata taaaccgccc gttgttacaa tgacgccatt gttaatggtt
     6781 aacttactac cctctcctgc aacggatacc cgtaacttcc attacccgtt gttataacta
     6841 gcgcattatc aatatctttt acttttccat cttttattgc cacagcatgt ttatattcat
     6901 cgtgctgaat aaactgttca gcatatgcat tacccaccgc cagagcaata agtcccaaac
     6961 taaacccggg gagttttttc agtttcatag attaactatc cattcattgg attaacaaac
     7021 attcctggtc aaataaccta tgagctcaga ttaccaaaac ggagggtaaa gacaaaaaca
     7081 aaagcacaaa aaatttaaat agataaacag cgacaatata taaatgataa ataagtcggc
     7141 attatcattt ttcaccttat tttcatgaca ttgatcactt tggggtgact attttgttta
     7201 acaaataact cttcgacatc aaaattatta atagttgcca tggtaattat ttcggtgata
     7261 tataaaaaca actgtgataa caccaaggtt gacaactatt tgcgcaataa ttcctttcct
     7321 tcaatgatcc aggcgttcac tgccagcaac ccattcagcc gcatactgac agtgagaaac
     7381 tgaaagatta ttgctgatcc ctgtggctaa cgagtctttc tcttaaatcg attaaccaga
     7441 ttgatcgcca gccaaacaag ggcattaagc aaatttatcg tcacaagcac ttctcccagc
     7501 ccggtactgg gttttgcaat aaggatgacc aggagggtga tcagcataaa tgcaatggaa
     7561 aagatgatat agagagtgaa ataatccaga ttattactat tattcatgct actaatggca
     7621 atcctgtcaa aactttcaaa caattatagc aaacaaaata atgaacaata gtaacaagag
     7681 gcaacgagct aattagctgt ctataattac accgcactat ggccttactg atcaattttc
     7741 actcaaccac aaccttcacc ttcggtttag aaaggataat tttgaccagg tcgatgtatg
     7801 taacaggttt atagaaataa taaccctgct gaaatgtgat attattttgg ttcagatagc
     7861 caagttgttc tttcgtttcg acaccttcag cgacgatact gatcgaaagt ttacgcgcca
     7921 gatccaatac acaatccaga attcgggttg attctgggtc tgcgtttacg cggccaacga
     7981 aactgtgatc aattttgata taatcaatat gcaggtcatg aagataagaa agccctgagt
     8041 agccagtacc gaaatcatcc agcgcgatga caaaaccatt ttcatgcaga atgttcaacc
     8101 gctgaaccag actttcatca acattcaatg gctcacgctc ggtgacttcc agaacaaggt
     8161 ttaaatcgcg gcgggtaaaa ctgtcacgga aatttaaaca ctcgtcgaca aacgtcggcg
     8221 aaataatatg cgaagcgcta aaattaattc caatatgaaa accttccggc agtttactcg
     8281 cgatagcgtt catctgtctg gcaacctgat tcatcaggct ttgtgtaagc ggaacgatta
     8341 atccggattt ttcagcaagt ggaataaatg ccgcgggtga aatatatcca ccgtgaggtt
     8401 gtttccagcg ggctaacacc tcaactcccc gcaatgtccc ttcccgacca tttaccacag
     8461 gttggtaaaa agggacgatc tccccattat ttattgcccg tcgcaggatc tcttcaggcg
     8521 ttgcgctttt attgaaatat ctatcaagca ggaaggcagc ggcgctggcg attaacagaa
     8581 taaatattaa aatcccaaag cctttatcga taagtctgta gaagctaaag agtggcggcg
     8641 ggttaaagat aatagtgaaa tgatattttg tggagtgaac cttctcggta tagggataat
     8701 gtccattaag cgtcatcaca tcagccgttg gtccaataat gtcatccgcc acgcgtaata
     8761 cataccttac ccctttcaag ggtacattaa gcgccccgcg aatatgttga tcacttatcg
     8821 taaccaaaat gcgcgtatct gcaaattggc tctgatagag caggataggt aatctattaa
     8881 cggtgtcgat tgccggagcc aacagtaaat tactgtccgg gacaacagga atacgagaca
     8941 gcaggacccg attcccaggc agggatgtac accagactat tccctgtttg agaacgatga
     9001 ttgtgcgcag gtgaggtttc agagctgctt cagtgccaag ctgatattgc tcctcgaggt
     9061 cgcattcctt cccggcaatg ttaaccgctg tttgagtcgc ctgtgacgct tcttcaagta
     9121 tatgattgat attgttggcg gcatatcttg ctcccgccag gtactctgct tttgcggaat
     9181 accagagctg gatgttcaaa atagcgacac cggtaataaa caagcatatc gcaacgagga
     9241 tgggtatgag tgtattgcgc atttgcttgt cccggccaaa gggttt
//
LOCUS       DAJTYH010000138         9204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTYH010000138 DAJTYH010000000
VERSION     DAJTYH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..663)
                     /gene="pflA"
                     /locus_tag="OO609_003526"
     CDS             complement(<1..663)
                     /gene="pflA"
                     /locus_tag="OO609_003526"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6634809.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDG"
     gene            complement(855..3137)
                     /gene="pflB"
                     /locus_tag="OO609_003527"
     CDS             complement(855..3137)
                     /gene="pflB"
                     /locus_tag="OO609_003527"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6634810.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3192..4049)
                     /gene="focA"
                     /locus_tag="OO609_003528"
     CDS             complement(3192..4049)
                     /gene="focA"
                     /locus_tag="OO609_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6634811.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4456..6216)
                     /locus_tag="OO609_003529"
     CDS             complement(4456..6216)
                     /locus_tag="OO609_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6634812.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6346..6972
                     /locus_tag="OO609_003530"
                     /pseudo
     CDS             6346..6972
                     /locus_tag="OO609_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7171..8259
                     /gene="serC"
                     /locus_tag="OO609_003531"
     CDS             7171..8259
                     /gene="serC"
                     /locus_tag="OO609_003531"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6634813.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8330..>9204
                     /gene="aroA"
                     /locus_tag="OO609_003532"
     CDS             8330..>9204
                     /gene="aroA"
                     /locus_tag="OO609_003532"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6634814.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGD"
BASE COUNT         2325 a         2211 c         2311 g         2357 t
ORIGIN      
        1 accatcgagt ttgtattctt cacccatcgc cacccatttg tgtttgccca gttcgtggta
       61 ggggagaagt tcgattttct caacgttacc catatcacgg gtaaattcac cgaggcgatg
      121 tgccgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc acaccttcac
      181 atttttgttc gccagatatt tagcgaactc cagcgtgcgg tggttggaaa ctccaaccag
      241 attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt cggttacttc
      301 cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtat ccagacaggt
      361 atgaatgcct tcttttttac aggcgcggaa ccagtcacga acaaactcag cttgcaggat
      421 tgcttcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt ggcgataggt
      481 caccacttcc ttcatcaaat cttcaacggt aacttcttta ccgccatgcg tgtcccaggt
      541 gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg tgataaagcg
      601 aatacctggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac caataactga
      661 cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc agctcgaagg
      721 ctacgtcgag tctgttttgg cagtcacctt aaagtataga tagctgacaa aaaaggctct
      781 cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta ctgtacgatt
      841 tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc ctgctgctgt
      901 tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt cagctgcgga
      961 tatttttccg ggttttccat cgcgtcgagc agcatttcac ggttcatcac gttaacgttc
     1021 aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat cagaccagcc
     1081 aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat agagaaggtg
     1141 taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt cagagacgct
     1201 acagcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa cggcgcgcca
     1261 gcacgacgac cgtctggggt gttaccagtt ttcttaccat acacaacgtt agaagtgatg
     1321 gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg aattttcttc
     1381 atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcatt gttaccaaac
     1441 tgcgggtatt cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc acgaatcggt
     1501 ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag accagcgata
     1561 ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga ggcttcgtag
     1621 ctgtacttgt cgtgcatgta gtggatgatg ttcagtgcag tgatgtactg tttagccagc
     1681 cagtccatga agtgatccat gcgctccatc acttcatcgt agctcaggag atcgcctttg
     1741 atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc gccgttgatt
     1801 gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat ttgtttacca
     1861 acgatcatcg ggcttacgca gcaagcgata gcgtagtcat cgttgttgaa gtccggacgc
     1921 atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt agcggcgaat
     1981 ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg ttccggagac
     2041 ggccccatag tgtacagggt gttcaggaaa cggaagctgt ttttggtaac cagggtacga
     2101 ccatcgaggc ccataccacc gatagattcg gttgcccaga ttgggtcgcc agagaacagt
     2161 tcatcgtatt ccggagtacg caggaagcga accatacgca gtttcatgac caggtggtca
     2221 accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg ttcgatgtac
     2281 acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg agacttaaca
     2341 gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt agccggacca
     2401 gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc gcggtgctgt
     2461 tcagcgattt cttcgcgcag acggatagtc tgttccaggt ttacgccgtt ttccagatca
     2521 gcctgtagag aggtgaactg agcgtatttg tctttcatca ggtagtcgat accgtacagc
     2581 gcaacgcgac ggtagtcacc gatgatacgg ccacggccat aagcatctgg cagaccggtc
     2641 agaacaccgg atttacggca acgcaggatg tccggagtgt aaacgtcgaa cacgccctgg
     2701 ttgtgagttt tacggtattc agtgaagatt tttttgatca tcgggtccag ttcgcggttg
     2761 tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag agcacgtttc
     2821 agcggagctt cagtctgcag accaacaact ttttccaacg ctttattgat gtagccagcg
     2881 tcgtgagagg tgatggtgga agcaacagcg gtgtcaaaat caactggcgc gtgagtgcgg
     2941 ttttccagtt taacaccttc cattactttg tcccacaggg tggtggtcgc ttcagtagcg
     3001 ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat gaagtcacgg
     3061 acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt ggctaacttt
     3121 tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt tactgcgtac
     3181 ttcgacaacc attaatggtg atcgttttca cgcaggtaaa tgacccagta tgtcaaccca
     3241 accaacaaac caccgccgat aatattaccg atcgtaaccg ggatcaggtt atcagtgatg
     3301 aagttcatca cggtcaggtg agaaaaattt tccggtgcag aaccgacagc ggtccagaat
     3361 tccggagatg cgaagtcgcg gattacaata cccatcggga tcataaacat gtttgcgata
     3421 ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccataat gaacgctttg
     3481 tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat caggtttgcc
     3541 aggataccaa gacagacggc ctcaataaaa gtatggtgca ctttgtggtc ggcggtttgt
     3601 aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag ccacattaaa
     3661 agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca gtttttagcc
     3721 aactgacacc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac tgtggaagta
     3781 aagagatcgg ctccgcagac aacacaaaga atcagcccca gagagaagca aatgccgcca
     3841 accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt gatatagaag
     3901 acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt cttaagcgga
     3961 tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc agcaggaagt
     4021 aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag tcggcgacga
     4081 gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc ctggcttata
     4141 ggcccgtaac tcgcatggtt tttatgcaaa tacggagtaa ctatttgatt atccaaaata
     4201 aaaataaatt ttaaaaatta acaaatgagt tgaatttttt ccgcatcctc cgctaaaaca
     4261 gttaattaaa agggagcatc aggcgaataa agtaacaata tcgatcgtat ttattaaata
     4321 caaattaccg atatttaacc ttataattac aattatttta ttaatgcaaa tatatgtaaa
     4381 gcggggcatt aaaaaaacgc cccgtaatat aactcagact aatcattaag cctacattgc
     4441 gtaggctatt tgattttatt ttgcccagaa tgctgctttg gcgcgctgca gcttttcgta
     4501 ggccttcaac aacgactgat gtgcagcaaa cgcgtgcaga tcgctatcta ccggttgcag
     4561 gccgtaaaac gccgcttcgc cgctcattgc cgcactggcg gcttctacgg catctgcgcc
     4621 gtacatgcga acaaacgcat tcagatattg cagcggctgg cgatcttctt cctgtgccag
     4681 taataacaac gtttgcaggc agcgataata gttggcgcgt tccggactaa atactgatga
     4741 gttaaactcc atcgtccatt cggtccagac cagagcctgt tccagatcgc caccagccag
     4801 cgccagcatg gcttttaatt caccgatacg cagggtgtac caaccgttat ccgacccggt
     4861 cgccagaccc aacagctcac gcacgcgggt aaagtcatca aaaccttctt catccagttg
     4921 ctcgatgagg ttcaggtaat cttctttttc ccactcgctg cctggtagcg aaagaatcgt
     4981 ttcacgtaaa tggctgccca tactgttatt cgcgagccac agatcttcag ccggataaat
     5041 atcggacatg ccaggcacga taatacggca agcataaacg cccagatgct cgtaatcggc
     5101 aatataaact tctttatctt ctttgttgaa gatagccatc agcgtagcaa actcttcttc
     5161 cgtggtgccg gagaaattcc agtccacaaa cggataatcg gcatcctgct tgaacaggtc
     5221 ccaggagatt aaaccgctgg aatcgataaa gtgcgtttcg aggttggtat gttcagcgac
     5281 ttcttcatca tcgaaggttg gcggagtaaa cacatccaaa tctttcaggc cacgaccttg
     5341 cagcagctcg gtcacggtac gttccagtgc tacgccaaaa tcaggatgcg caccgaaaga
     5401 ggcaaagcag gtgccgtttg caggattaaa cagtaccacg caaatcaccg gatactggcc
     5461 gccaagggaa ccatcatatg cgaagatcgg gaaaccttct gtttccagtg tttcgatcgc
     5521 ttcaaccacc gctgggtaac gcgccagcac gtctgccggg atttccggca agctgatgct
     5581 ttcagcaata atgcggtttt tcacgtagcg ttcgaaaact tcggacaacc cctgaacgcg
     5641 tgcttcgttg cgggtattgc cagcggacat accgttagaa acgtacaggt taccaatgat
     5701 attcatcgga atataaacgg tctgattgtc ggactggcgc gtaaacggca gaccgcaaat
     5761 accacgatct tcgttaccgg attgtaggtc aatcagcata ctgccggtca attcattctc
     5821 cggatcgtaa aatgcgcgca gacggtcatc gagcagccct tctggcacat cgtcattttc
     5881 gctcagtggg aaccattttt cgttgggata atgcacaaat ggaccgttgg cgatggtttc
     5941 gcccagccag aaatcagcaa aaaagtagtt ggttgagaga cgctcgaaat attcaccgag
     6001 tgcagaagcc agcgccgctt tcttggttgc gcctttaccg ttggtaaaac acagtgcgca
     6061 ctctttgtcg cgaatatgta cagaccagac gttaggcacg ggattcagcc aggaggcctc
     6121 ttcaatctga aagccgaggt ctgaaagttt ttgctggaag cgagcgatgg aatcttccag
     6181 agcggcatct ttgccgggga taaatgtttg cgtcatgaaa atcactttag tcgtacggaa
     6241 agcgcgcaat aatacgggtt ttatctcaaa ggcgctatca ccgccgccat gccgatgaac
     6301 tgttgactat gcttttagcg gataaaccac gttaagagca taaaaatgaa ggcgttcgat
     6361 ctccaccgta tggcatttga taaagtgcct tttgatttcc ttggcgaagt tgcactacgt
     6421 agtctttata cctttgtact ggttttttgt tcctcaaaat gaccggaaga cgcggtgtgc
     6481 gacagatgtc gctgtttgaa gtgttaatca ttctcacgct gggttcagcg gcgggagatg
     6541 tggcgtttta tgatgatgtg ccgatggtgc cggtacttat cgtctttatc actctggcgt
     6601 tgttataccg cctggtaatg tgatggcgag ctggcctggt cgaaactcaa taactccaac
     6661 atgacggaat ttgagttctt tatggagcta cgattgcgtg gcgtggagca gctggggcag
     6721 gtacgtctgg cgattctcga aaccaacggg caaatcagtg tctatttctt tgaagatgac
     6781 aaggtgaaac cgggtttact tattttaccc agtgattgta cgcagcgtta caaagtggtg
     6841 ccggagtcgg cggactatgc ctgcattcgt tgtagtgaaa tcattcatat gaacgcgggg
     6901 gaaaaacaat tatgtccgcg ctgtgcaaat ccagaatgga cgaaggcaag tcgggcaaaa
     6961 cgggtgacct gacagtaaaa acatcggctt tttgctaata atccgagaga ttcttttgtg
     7021 tgatgcaagc cacatttttg ccctcaacgg ttttactcat tgcgatgtgt gtcactgaat
     7081 gataaaaccg atagccacag gaataatgta ttacctgtgg tcgcaatcga ttgaccgcgg
     7141 gttaatagca acgcaacgtg gtgaggggaa atggctcaaa tcttcaattt tagttctggt
     7201 ccggcaatgc taccggcaga ggtgcttaaa caggctcaac aggaactgcg cgactggaac
     7261 ggtcttggta cgtcggtgat ggaagtgagt caccgtggca aagagttcat tcaggttgca
     7321 gaggaagccg agaaggattt tcgcgatctt cttaatgtcc cctccaacta caaggtatta
     7381 ttctgccatg gcggtggtcg cggtcagttt gctgcggtac cgctgaatat tctcggtgat
     7441 aaaaccaccg cagattatgt tgatgccggt tactgggcgg caagtgccat taaagaagcg
     7501 aaaaaatact gcacgcctaa tgtctttgac gccaaagtga ctgttgatgg tctgcgcgcg
     7561 gttaagccaa tgcgtgaatg gcaactctct gataatgcgg cttatatgca ttactgcccg
     7621 aatgaaacca tcgacggtat cgccatcgac gagacgccag acttcggcaa agatgtggtg
     7681 gtcgccgccg acttctcttc aaccattctt tcccgtccga ttgacgtcag ccgttatggt
     7741 gtaatttacg ctggcgcgca gaaaaatatc ggcccggctg gcctgacaat cgtcatcgtt
     7801 cgtgaagatt tgctgggcaa agcgaatatc gcgtgtccgt cgattctgga ttattccatc
     7861 ctcaacgata acggctccat gtttaacacg ccgccgacat ttgcctggta tctatctggt
     7921 ctggtcttta aatggctgaa agcgaacggc ggtgtagctg aaatggataa aatcaatcag
     7981 caaaaagcag aactgctata tggggtgatt gataacagcg atttctaccg caatgacgtg
     8041 gcgaaagcta accgttcgcg gatgaacgtg ccgttccagt tggcggacag tgcgcttgac
     8101 aaattgttcc ttgaagagtc ttttgctgca ggccttcatg cactgaaagg acaccgtgtg
     8161 gtcggcggaa tgcgcgcgtc tatttataat gccatgccgc tggaaggcgt taaagcgctg
     8221 acagacttca tggttgagtt cgaacgccgt cacggttaat gccgaaattt tgcttaatcc
     8281 ccacagccag cctgtggggt ttttatttct gttgtagaga gttgagttca tggaatccct
     8341 gacgttacaa cccatcgctc gtgtcgatgg cactattaat ctgcccggtt ccaagagcgt
     8401 ttctaaccgc gctttattgc ttgcggcatt agcacacggc aaaacagtat taaccaatct
     8461 gctggatagc gacgacgtgc gccatatgct gaatgcatta acagcgttag ggttaagcta
     8521 tacgctttca gccgatcgta cgcgttgcga aattatcggt aacggcggtc cattacacgc
     8581 agaaggtgcc ctggagttgt tcctcggtaa cgccggaacg gcaatgcgtc cgctggcggc
     8641 agctctttgt ctggatagca atgatattgt gctgaccggt gagccgcgta tgaaagagcg
     8701 cccgattggt catctggtgg atgcgctgcg cctgggcggg gcgaagatca cttacctgga
     8761 acaagaaaat tatccgccgt tgcgtttaca gggcggcttt actggcggca acgttgacgt
     8821 tgatggctcc gtttccagcc aattcctcac cgcactgtta atgactgcgc ctcttgcgcc
     8881 ggaagatacg gtgattcgta ttaaaggcga tctggtttct aaaccttata tcgacatcac
     8941 actcaatctg atgaagacgt ttggtgttga aattgaaaat cagcactatc aacaatttgt
     9001 cgtaaaaggc gggcagtctt atcagtctcc gggtacttat ttggtcgaag gcgatgcatc
     9061 ttcggcttct tacttcctgg cagcagcagc aatcaaaggc ggcactgtaa aagtgaccgg
     9121 tattggacgt aacagtatgc agggtgatat tcgctttgct gatgtgctgg aaaaaatggg
     9181 cgcgaccatt tgctggggcg atga
//
LOCUS       DAJTYH010000139         9167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTYH010000139 DAJTYH010000000
VERSION     DAJTYH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..925
                     /locus_tag="OO609_003533"
     CDS             <1..925
                     /locus_tag="OO609_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6634815.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1177..2094
                     /gene="oxyR"
                     /locus_tag="OO609_003534"
     CDS             1177..2094
                     /gene="oxyR"
                     /locus_tag="OO609_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6634816.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2077..3477)
                     /gene="sthA"
                     /locus_tag="OO609_003535"
     CDS             complement(2077..3477)
                     /gene="sthA"
                     /locus_tag="OO609_003535"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6634817.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3798..4448
                     /gene="fabR"
                     /locus_tag="OO609_003536"
     CDS             3798..4448
                     /gene="fabR"
                     /locus_tag="OO609_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6634818.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4448..4807
                     /locus_tag="OO609_003537"
     CDS             4448..4807
                     /locus_tag="OO609_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6634819.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4847..5947)
                     /gene="trmA"
                     /locus_tag="OO609_003538"
     CDS             complement(4847..5947)
                     /gene="trmA"
                     /locus_tag="OO609_003538"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6634820.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6061..6251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6316..8160
                     /gene="btuB"
                     /locus_tag="OO609_003539"
     CDS             6316..8160
                     /gene="btuB"
                     /locus_tag="OO609_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6634821.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8105..8962
                     /gene="murI"
                     /locus_tag="OO609_003540"
     CDS             8105..8962
                     /gene="murI"
                     /locus_tag="OO609_003540"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6634822.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2093 a         2219 c         2474 g         2381 t
ORIGIN      
        1 tctgggtatt ggcgaagcgc catttatgcc tgcgggagta aagtcgatca ccgactggta
       61 tgcacaaaaa gaacgcggca cggcgctggg gatttttaac tcatctaccg ttatcggtca
      121 ggctatcgcg cctcctgctc ttgtattgat gcagctggca tggggctggc ggacgatgtt
      181 cgttatcatc ggcgtggcag ggattctggt tgggatctgt tggtacgcgt ggtatcgcaa
      241 ccgggcgcag tttgtcctga ctgacgaaga acggacgtat ctctccgcgt cggttaaacc
      301 gcgtccacag ctgcaattta gcgagtggct ggcgctgttt aagcatcgga caacctgggg
      361 gatgatttta ggtttctctg gcgtcaatta taccggttgg ctttacattg cgtggttacc
      421 cggttatttg caagccgaac aaggtttcag tctggcgaaa accggttggg tagcggcgat
      481 tcctttcctc gcggcggcgg tcgggatgtg ggttaacggt attgtggtcg atcggctggc
      541 gaaaaaaggc tacgacctgg cgaagacgcg taaaacggct attgtctgcg gtttgatgat
      601 gtcggcatta ggcacgttgc tggtcgtgca atcttcctcg ccagctcagg cggtagcgtt
      661 tatctcaatg gcgctgttct gcgtgcattt cgcgggaacg tctgcatggg gactggtgca
      721 ggtgatggtg tcagaaacaa aagtggcttc catcgccggg attcaaaact ttggcagttt
      781 tgtttttgct tcctttgctc cgatcgtaac cggttgggta gtggatacca cacactcgtt
      841 taatctggcg ctggttatcg cggcctgtgt gacgttcacc ggggcgctgt gttacttctt
      901 tattgtcaaa gatcgcattg agtaatcgtt actatcaggc tctcttgctg tgggcctgta
      961 gaataaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
     1021 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
     1081 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
     1141 atcgccatga actatcgtgg cgatggagga tggataatga atattcgtga tcttgagtac
     1201 ctggtggcat tggctgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
     1261 cagccgacgc ttagcggcca aattcgtaag ctggaagatg agctgggcgt gatgttgctg
     1321 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt ggatcaggcg
     1381 cgtaccgtgc tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggtgagacg
     1441 atgtccggac cgctgcatat tggtttgatt cctacggttg gaccatacct gctaccgcat
     1501 attatcccga tgctgcacca gacatttcca aagctggaaa tgtatctgca tgaagcacag
     1561 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
     1621 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
     1681 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac tgatggccga tctggcaggg
     1741 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgc
     1801 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga aactctgcgc
     1861 aacatggtgg cggcaggtag cgggatcact ttattgccag cgctggctgt gccgccggag
     1921 cgcaaacgcg atggggttgt ttatctgcca tgcattaagc cggaaccatg ccgcactatt
     1981 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
     2041 atccgcgcaa gaatggatgg ccatttcgat aaagtgttaa aacaggcggt ttaaaccgtt
     2101 taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa
     2161 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
     2221 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
     2281 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcacgtgcca gatgtttgaa
     2341 ctgggcgcgg cccacttcat acggcacttt catcgcggtc agctgctgtt cggttttgcc
     2401 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc
     2461 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
     2521 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
     2581 atacatgctg ttgaccttca gctgtccgcg actgtcagtt tccagcccaa tgttctgtaa
     2641 cgccagcgaa tcggtattac cggtacgacc gttggcatag agcaggcagt cagctttcag
     2701 ttttttaccc gacttcagat gcatgatcac accgtcgtca cagctttcga tcttctcgta
     2761 ctcttcgttg tgacgaatca ctacgccact gttccagaag tggtaggaga gagaatctga
     2821 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
     2881 atccatacct cggaagatcg acgcatattc acagccgatc actccagcac catagataag
     2941 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
     3001 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
     3061 agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
     3121 aaagcgagcg ttcccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
     3181 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
     3241 gagcagtcgg gaatggtcgc tgtatagtgg gttttgattg aattctataa tgcggctgac
     3301 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
     3361 ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca ttgcagcgcc
     3421 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
     3481 agggcttacc tgttcttata cataaaagca acagaatggt aacattctat cgcgggtaag
     3541 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
     3601 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
     3661 ggtttccgct gtacgtaaaa gaaccggcca gtaaatatgt tttattgcgt taccgttcat
     3721 tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc agtacttttg
     3781 gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca aaaagaaaaa acccgccgtt
     3841 cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcgcc agcctgagtt
     3901 tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg catttccgcg
     3961 acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta cgccaactca
     4021 tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgtacc tcggtctcca
     4081 catttatgga gttcatcggt aataatccta acgccttccg gttattattg cgggaacgct
     4141 ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac ttcattgcgg
     4201 aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact gaagcgcaag
     4261 ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac gtcggcgtcg
     4321 aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt tcgaaagggg
     4381 cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga aatgtgaagg
     4441 acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt tggttgctgg
     4501 tttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat tttctgtatt
     4561 cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc ttaatcgcac
     4621 catgtcggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg tactgctgta
     4681 cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc cggcagtact
     4741 ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc aggaagttgc
     4801 tgagtaatcg gtatcatgcc ggatgctggc gcatccggca tgggttttac ttcgcggtca
     4861 gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga gccagacgtt
     4921 cgaccttgtg cgtctggctt aatgtttcca gatttttgca taacgtttcc ggattgcagg
     4981 agatatacag aatacgtgga tacgcctgca ccattttctc ggtttcactg tccagaccgc
     5041 tgcgcggtgg gtcgacaaaa atggtttcga actgatagct ctttaagtcg atcccttgca
     5101 ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc gccatacgaa
     5161 taatttgtac gttatcaata tggttagcgg caatgttgta ttgcgcggca gcgactgacg
     5221 gtttggcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct aatgaaaagt
     5281 taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt acgtccagcg
     5341 cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg ttttctacct
     5401 gacgatagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa tcttgatcca
     5461 gcgcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc tgcgcgcgca
     5521 gtgcatcgcg cagtgcttcc gcttcctgac gccactcatc atccagcttt ttatggtaca
     5581 gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg aacaacttgt
     5641 ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac tggttgatga
     5701 gttcactggc ggcggggaag ctatccacgc ggatgcggct tttggtttgt tgatcgaaaa
     5761 tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcgcgc atccggtaat
     5821 gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc atcatacttt
     5881 gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga aggtgttcgg
     5941 gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt gctcatcaga
     6001 tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac ttgttacaac
     6061 ctgtagcatc cacttgccgg tcctgtgagt taatagggaa tccagtgcga atctagagct
     6121 gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc catccggtgg
     6181 gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct gccggccaac
     6241 gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc cttcttctat
     6301 tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc cgtcacggca
     6361 ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc tatccgtttt
     6421 gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca ggatatcgac
     6481 cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt cgatatcacc
     6541 caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa tgccagtcat
     6601 gtgctggtgt taattgatgg cgtccgcctg aatctggcgg gggtgagtgg ttctgccgac
     6661 cttagccagt tccctattgc gcttgtccag cgtattgaat atatccgtgg accgcgctcc
     6721 gctgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac gcgcgatgaa
     6781 cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta tgatgtctct
     6841 acgcagcaac aactggggga taaaacacgg gtaacgctgt tgggcgatta tgcccatact
     6901 catggttatg atgttgttgc ctatggtaat accggaacgc aagcgcagac agataacgat
     6961 ggttttttaa gtaaaacgct ttatggggcg ctggagcata actttactga tgcctggagc
     7021 ggctttgttc gcggctatgg ctatgataac cgtaccaatt atgacgcgta ttattctccc
     7081 ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc cgggctgcgc
     7141 tataacggcg aactgattaa atcacaactc attaccagct atagccatag caaagattac
     7201 aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga gatgaagcaa
     7261 tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg tgcgggtgtc
     7321 gactggcaga aacagactac gacgccgggt acaggttatg ttgaggatgg atatgatcaa
     7381 cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac ctttgaaggc
     7441 gcagcacgca gcgacgataa ctcacagttt ggtcgtcatg gaacctggca aaccagcgcc
     7501 ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc ttataaggca
     7561 ctaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc ggagaaaagc
     7621 aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg tatttccgga
     7681 tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa atattacaac
     7741 gaagggaaag cgcggattaa gggtgtcgag gcgacagcca attttgatac cggaccactg
     7801 acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga cacgccgttg
     7861 ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta tgacttcgac
     7921 tggggtatta cttatcagta tttaggcact cgctatgata aggattactc atcttatcct
     7981 tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc gtatccggtc
     8041 acctctcacc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga ttatgagaca
     8101 gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta caccttctga
     8161 accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg tctatgacga
     8221 gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg tcgctttccc
     8281 gtatggcgaa aaaagcgaag cgtttattgt tgagcgagtg gtggcaattg tcaccgcggt
     8341 gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacaccgcca gtaccgtttc
     8401 acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc cggcgattaa
     8461 acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc gcggaacagt
     8521 taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc agatagaaat
     8581 gctgggctcg gcagagatgg ttgagttggc tgaagcgaag ctgcatggcg aagatgtttc
     8641 tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc cgccagatac
     8701 cgttgtattg ggttgcaccc atttccctct actacaagaa gaactgttac aagtgctgcc
     8761 agagggaacc cggctggtgg attctggcgc agcgattgct cgccgaacgg cctggttgtt
     8821 agaacatgaa gccccggatg caaaatctgc cgatgcgaat attgcctttt gtatggcaat
     8881 gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg aaacgctcga
     8941 aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcggaaaa ttattttaaa
     9001 tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg
     9061 gcaaacacgc cgacgggtca gcggggttct cctgagaatt ccggcagaga aagcaaaaat
     9121 aaatgcttga ctctgtagcg ggaaagcgta ttatgtacac cccgcgc
//
LOCUS       DAJTYH010000140         9112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTYH010000140 DAJTYH010000000
VERSION     DAJTYH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            383..535
                     /gene="hokE"
                     /locus_tag="OO609_003541"
     CDS             383..535
                     /gene="hokE"
                     /locus_tag="OO609_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6634823.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(657..1277)
                     /gene="entD"
                     /locus_tag="OO609_003542"
     CDS             complement(657..1277)
                     /gene="entD"
                     /locus_tag="OO609_003542"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6634824.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1452..3692)
                     /gene="fepA"
                     /locus_tag="OO609_003543"
     CDS             complement(1452..3692)
                     /gene="fepA"
                     /locus_tag="OO609_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6634825.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3935..5137
                     /gene="fes"
                     /locus_tag="OO609_003544"
     CDS             3935..5137
                     /gene="fes"
                     /locus_tag="OO609_003544"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6634826.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5140..5358
                     /gene="ybdZ"
                     /locus_tag="OO609_003545"
     CDS             5140..5358
                     /gene="ybdZ"
                     /locus_tag="OO609_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6634827.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5355..>9112
                     /gene="entF"
                     /locus_tag="OO609_003546"
     CDS             5355..>9112
                     /gene="entF"
                     /locus_tag="OO609_003546"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6634828.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDI"
BASE COUNT         1973 a         2417 c         2521 g         2201 t
ORIGIN      
        1 gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata
       61 tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga
      121 tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac
      181 aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg
      241 cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat
      301 atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc
      361 ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct
      421 gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt
      481 aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt
      541 gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt
      601 acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa
      661 tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc
      721 tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga
      781 aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca
      841 agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt
      901 gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca
      961 acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg
     1021 ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta
     1081 tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta
     1141 cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca
     1201 caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag
     1261 gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga
     1321 tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt
     1381 aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc
     1441 tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta
     1501 taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg
     1561 gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg
     1621 acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct
     1681 ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg
     1741 ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt
     1801 gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc
     1861 ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac
     1921 gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg
     1981 ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta
     2041 cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg
     2101 ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg
     2161 gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc
     2221 ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc
     2281 gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc
     2341 cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa
     2401 atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg
     2461 gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc
     2521 cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg
     2581 ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta
     2641 aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca
     2701 tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg
     2761 tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca
     2821 gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc
     2881 agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg
     2941 atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga
     3001 tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg
     3061 aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct
     3121 tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg
     3181 ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct
     3241 gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca
     3301 ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc
     3361 ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg
     3421 ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg
     3481 atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc
     3541 gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt
     3601 gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc
     3661 aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa
     3721 ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt
     3781 gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc
     3841 attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca
     3901 atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga
     3961 gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga
     4021 ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt
     4081 atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca
     4141 gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg
     4201 cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg
     4261 cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa
     4321 tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc
     4381 gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat
     4441 ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt
     4501 aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat
     4561 gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta
     4621 tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga
     4681 tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag
     4741 cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta
     4801 tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg
     4861 gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga
     4921 agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat
     4981 gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg
     5041 tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat
     5101 cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc
     5161 gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg
     5221 ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag
     5281 tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag
     5341 ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga
     5401 tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa
     5461 ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag
     5521 atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg
     5581 cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta
     5641 ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac
     5701 cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt
     5761 atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca
     5821 tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg
     5881 atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat
     5941 tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag
     6001 cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat
     6061 tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga
     6121 cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta
     6181 tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc
     6241 tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc
     6301 gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg
     6361 tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta
     6421 aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa
     6481 ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta
     6541 ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac
     6601 gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg
     6661 atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga
     6721 ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc
     6781 cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg
     6841 cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac
     6901 taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct
     6961 ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc
     7021 gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt
     7081 taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt
     7141 cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag
     7201 gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc
     7261 cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt
     7321 gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc
     7381 accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact
     7441 ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga
     7501 gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc
     7561 gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg
     7621 cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca
     7681 gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc
     7741 atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg
     7801 ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg
     7861 gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt
     7921 acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa
     7981 tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga
     8041 ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc
     8101 aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc
     8161 cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca
     8221 agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc
     8281 cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg
     8341 tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac
     8401 tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg
     8461 cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca
     8521 tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc
     8581 atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat
     8641 ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc
     8701 tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg
     8761 cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc
     8821 cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg
     8881 cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg
     8941 agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc
     9001 atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag
     9061 gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc ta
//
LOCUS       DAJTYH010000141         9087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTYH010000141 DAJTYH010000000
VERSION     DAJTYH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..798)
                     /locus_tag="OO609_003547"
     CDS             complement(<1..798)
                     /locus_tag="OO609_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6634829.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDET"
     gene            858..1136
                     /locus_tag="OO609_003548"
     CDS             858..1136
                     /locus_tag="OO609_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634830.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1137..1664)
                     /gene="gadE"
                     /locus_tag="OO609_003549"
     CDS             complement(1137..1664)
                     /gene="gadE"
                     /locus_tag="OO609_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6634831.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2463..3035)
                     /gene="hdeD"
                     /locus_tag="OO609_003550"
     CDS             complement(2463..3035)
                     /gene="hdeD"
                     /locus_tag="OO609_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6634832.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3290..3622
                     /gene="hdeA"
                     /locus_tag="OO609_003551"
     CDS             3290..3622
                     /gene="hdeA"
                     /locus_tag="OO609_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6634833.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3738..4064
                     /gene="hdeB"
                     /locus_tag="OO609_003552"
     CDS             3738..4064
                     /gene="hdeB"
                     /locus_tag="OO609_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6634834.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4128..4774
                     /locus_tag="OO609_003553"
                     /pseudo
     CDS             4128..4774
                     /locus_tag="OO609_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4816..5346)
                     /locus_tag="OO609_003554"
     CDS             complement(4816..5346)
                     /locus_tag="OO609_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6634835.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5502..6068)
                     /gene="slp"
                     /locus_tag="OO609_003555"
     CDS             complement(5502..6068)
                     /gene="slp"
                     /locus_tag="OO609_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6634836.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6315..7538)
                     /locus_tag="OO609_003556"
     CDS             complement(6315..7538)
                     /locus_tag="OO609_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634837.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8119..>9087)
                     /locus_tag="OO609_003557"
     CDS             complement(8119..>9087)
                     /locus_tag="OO609_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6634838.1"
                     /translation="AEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGR
                     GYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTF
                     GWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIK
                     VSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFI
                     YGFIPSAWIAAHAGSDNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMAL
                     IIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2656 a         1796 c         1897 g         2738 t
ORIGIN      
        1 ggtttcatca accgtcgggt cggagaattt cagcgtgccg gtctggctgt aacgtttacc
       61 attttccaga ttgagctgta ctggcgtact gccctgaacc tgtttgattt gcccactggc
      121 gacctcttct ttcatgcgta agaaatcttg caccgactgc gtgagatcga cataaatcgg
      181 gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac
      241 ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag gtgacattcg cgtattgcag
      301 attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg acattggctt ctgcttcatt
      361 cagctgggtg cgcgcggtgt cgtaatcctg acggctaacg tagttggtct tcagcaacga
      421 tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta gagagcgctt tcgccagcga
      481 gcctttggcg gagtttagct cggcctgtaa aggtgcagga tcaatctgat acagcgaatc
      541 gccctggttc actttatcgc cttcgataaa gttgcgttta atgataatac cgcccacctg
      601 gggacgtatc tcggcaactt cataaggaac ggttctaccg ggcaattcgc tcaacacatt
      661 gaccgaacca ggggagagtg tgacgacacc gacctcaggc gtcatggcgg cggcgttttc
      721 cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag aataacaacg gtattaacag
      781 ctttcttctt ctgttcattt tagtccctga aaattcttga gaaataacgg ttctttaaat
      841 tgcgcgcgag gagagatatg agggatttac agaccagcgg catagtgggg ttgtcagcca
      901 gtaaggtggg atacaggcac agtgatcgac atggtgaggt caacgacaaa aaaaatgttg
      961 ccgttctgcc aacagttcct gccagcattc gggcaacgaa gggttctact ggtggataca
     1021 cataccaggg gaatcaagtc gttttagcct ctcctttagt aatcaccggg ggtaatgaaa
     1081 tcagcatctg catccctcgt catgccagcc atcaatttca gttgcttatg tcctgactaa
     1141 aaataagatg tgatacccag ggtgacgatg tcgctcatac gtcttagttt cagcttgtac
     1201 ataatgttga atttctgaat ctttaatgtt tgttgcgaaa tattgagcgt cgacgtgata
     1261 ttgctttcat ttttattttt cagaatcagt tgcataattt tgaactgatt atgtgaaaaa
     1321 cataattgtg aattcttatg gggcaagtgt ttaccataag taatctcaag aagatttttg
     1381 atactttctt tgcggctaac aaaataaata ggcgctttag cttttagttt actgatgtga
     1441 taaggtgcca aaacaataat tctctcggca tctaatttct ccagaaattt aatcgcttct
     1501 tcatcaagga tatgattgtg ataggtatca atgataacct tgaagggttt attgcctact
     1561 tttttgatct ctgacaggga attgattttt atcatttcgt gattatcttt caactgccaa
     1621 aagccctgta aaagaaaaga atctttcgtc atgagaaaaa tcataacttg ctccttagcc
     1681 gttatcgttt cgaacatgtc atccttttca ttatttacat ccttgtccga atcgttgttc
     1741 aatatagtaa acgcctataa gaactttgcc cttcgtgaac taatacgtct attaacacta
     1801 atcaatactt cagatttatc catcacgcac tccatatatt gatcctaagt aagattcctg
     1861 gttgttatca gcttgtaaat agtaaacaaa aagttaacgt ttgcggatta ctgcccaaga
     1921 ataagttatc aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat
     1981 ccgatttaaa tatcgagtct ccttgtttcg acttaagctg gcaattggat tgccagcttt
     2041 cttttaacgc attgaaatta cctgagtaaa taacgcattc atgtcttcag gtttttatat
     2101 agcgtctgga gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac
     2161 ccggattact tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc
     2221 ccacagcact tttaaaacat taaacctgac tacggaaaat atcagccatg ataatgtttg
     2281 aaatggctaa tttgccatgg agtgaaaaaa attagatgaa attcagtagg ttgaaataat
     2341 cactagcagg taattatttc aatgatagtg cgcaattgat ctacaacact gcgtagcgga
     2401 gagagtatta atcggatcat agtcacatca agtgactatg atccgggtga caaccggggt
     2461 aattattgct gcttaacgaa caaactggcg aagctgaaca ggctggcggc gctaaatatc
     2521 agttcaattc ccaccagtgt ggaaaccagc gttacagaca ccatcggcgt tgcaccaagg
     2581 aatatccagg caatgacgat atccagcaca ccaataacga gctgtagcca gctgcctttc
     2641 attgaacgct gacgatacca actcatcagg cgaataaccc ctgcaacaca gaacaaaccg
     2701 gcaataaatg ccgcaatggc aaaaatgccc agctccggtg cacggatgaa gaaatagccg
     2761 atcaataaat aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg
     2821 cggttgctga ataacccgac aataagcgca atacccgagc agattaataa tgcacccact
     2881 actgtgctta aaatatcgcc agagacgaac gggaaactga tacacagcaa cccgacgata
     2941 aacagcagca cggcaataaa ctggattgct ctgcgatgct ttttcagcat ctccagatca
     3001 aacttcaaaa ttgttgcctt atctatatat aacatagaac caccctataa aattaagaag
     3061 aaaatcccct gctatcaatc tatgccaaaa acgcgtctaa gaatgcagtc gatttaataa
     3121 aaatttccta attgcagtat ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc
     3181 tgattttgat attttccatc aacatgacat atacagaaaa ccaggttata acctcagtgt
     3241 cgaaattgat tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt
     3301 attaggcgtt attcttggtg gtctgcttct tctgccagtt gtgagcaatg cagcggatgc
     3361 gcaaaaagca gctgataaca aaaaaccggt caactcatgg acctgtgaag atttcctggc
     3421 tgtggacgaa tccttccagc caactgcagt tggttttgct gaagcgctga acaacaaaga
     3481 taaaccagaa gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt
     3541 tcaggcttgt actcaggata aacaagccaa ctttaaagat aaagttaaag gcgaatggga
     3601 caaaattaag aaagatatgt aattccggga atgcgttaca tcgtacttcc ttgcatattg
     3661 aacaggccgg aatatcttct ttaaaagcag ctattcctcc tgttcatata taatctctat
     3721 attgaatggg ttacaaaatg aatatttcat ctctccgtaa agcgtttatt tttatgggcg
     3781 ctgtagcggc tttgtcactg gtgaacgcac aatctgcgtt ggcagccaat gaatctgcta
     3841 aagatatgac ctgccaggaa tttattgatc tgaatccaaa agcaatgacc ccggttgcat
     3901 ggtggatgct gcatgaagaa acagtatata aaggtggcga taccgttact ttaaatgaaa
     3961 ccgatctcac tcaaattcct aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt
     4021 tgtatacctt caaaaatcaa gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa
     4081 aggagtagca agttgagcca tcttgctgct cctttttgca tttttatatg acagcagaat
     4141 ttattatacg tcttatactc gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa
     4201 tgcgcggcaa aggagcaggg ttacgcacac atgtattaat tggcatggga agcgccctgt
     4261 ttatgattgt ttcgaaatat ggttttgctg acgtgctgtc tttagatcac gtcggactcg
     4321 accccagccg tattgctgct caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca
     4381 ttctcgttcg taaccaaaat attgtaggtc tgacgacggc agcggatatc tgggtgaccg
     4441 ccgcataggt atggttattg gcagcggtat gtacgaactg ggtatttatg gttcagtgat
     4501 gaccttgttg gtgctggaag tcttccatca attaaccttc cgcctgatga acaaaaatta
     4561 ccatttacag ctgacattag tgaacggtaa tacggtatca atgctcgact ggtttaagca
     4621 gcaaaaaata aaaactgatt tagtttcgtt gcaggaaaat gaagatcatg aagtggtcgc
     4681 cattgatatc cagcttcatg ccacaacgtt gatagaagac ctgttgcgat tattaaaagg
     4741 tatggcaggg gtgaaaggcg tttcaattag ctaatctgtg tggcagcgta gccagactca
     4801 ccgtaagcct gaaattcaca ccagataatc aatatgctga tggcgtacca gctcattgat
     4861 ccgcttaaga ttgaggatca tcatgatatt gtgtttatgg cagtagaacg tttttagcga
     4921 tattttcagt ttctccagaa tatcttcagt tgtcatttga ttcgcaatgc agctcagaac
     4981 ctgatgttgc ttgcgcgaaa gtgacacatt cgcttcaggc acagagcagt aactgccatt
     5041 gatgagtgcg acaaaatcaa tgatagtgat agtccgcgga acaaaggtca ccggtacttt
     5101 ccccagacag cgtttaatat taaaggggga gaaaacaatg acatggacag gttttaatcg
     5161 ttcaatttga gtgagaaaat tagaatgaaa tacattattc attaatgtat caataatgac
     5221 gaaggcattc tgatgcaaca ttacgtcgat ctcttcttcg ttctgtatat gaacgacatt
     5281 acctttactc agaatgtttt tcatcgcggt gaagaacatc gtatccctgg taattataag
     5341 aaacataacc gactcctggt gcggacatta actttataat cagtttgcca aaggtaatta
     5401 acaggacagg gcttataata ataatagtta gcttactgta tattaatttt atgtaataca
     5461 tgatgcagat gctgagaaag catctgcatc tttcggtggt gttatttgac cagctcaggt
     5521 gttacctgac tcaccgcatt ggtgtagtaa ggcgcacccc agcccggttc cggccagaat
     5581 gcaccatagc cgtaatccca caggttatag gtggtattaa ccacttctct caaatgccac
     5641 acctggatgc cctgcatatt cacttccagg aagttatacg ggactttatt gataaagcct
     5701 ggttgttcac cctgaatggt accgaggatg gtaacaaagt gattacgata gttcactgga
     5761 tcaaggaagc cgctttgtct ggcgagcagt cggccctgat agttggcttc aatatcaggc
     5821 ttcgcatagc tatccaacgg taatacagcg atttctaaca acgtatccgt tttgccattg
     5881 ataacgttga taaccttccc accaaagcgc gcttgttgac caacatataa ccccggctgg
     5941 ttatgaacag caacaaaact tttttgaata tcaggttggt tattgccttt gatattttgc
     6001 ggaattgaac tacatgcggc aagcaaaaat gaaaggctga ggatgagtgc accttttgtc
     6061 atgttcatgt tactatcctt atcaacaaat attaaaacca taatagatat ttctttatag
     6121 ttttcatctg attctgaggt gatatgcctg atagtaacgt aaacctgtct gatgtttata
     6181 tcccttctgt ttagttcaat tccgttattt tggggcgtgg tatgagtatc catcgcccaa
     6241 taatataacc ctttgtttta aaattacata aaatctattt ttattcaatg attataaagt
     6301 gctaataatc aatattatgg tattatattc tgataatgat ttgataagcg aattcttttg
     6361 ccagatggta tttctatttc caccccataa tttatatctt tttcattagg cagatctctc
     6421 tttaaataaa aggggtatga aatcttttcg ggttgaagtg tctcgtaatg aaattctatg
     6481 aattcatttt tagacattgt tgcccatttt tgaattttat tgtatagaga gggtgttgta
     6541 aactcttgaa gttgtttata acatattttt ttgaactctt gcttatattt taatatgtca
     6601 tcaaatgaaa gattcactac ctttgcctct aagaaatcaa ttattctcga agcaatttcc
     6661 gctttcgcgc gtaaagagtt gtcattaata gttaacaatg tagaaaacaa accattgatt
     6721 tgtttgtcaa gatgttgttc atatttatgt agtttgggga tgggattttc cttgtgtgaa
     6781 aaaatagtgt taaataaatt tgcgtcagtg aaatttatat taagtagatc ttcaaaataa
     6841 aattttgata aggttaggac tagcttggac atatcttgcc ctgtcatgtt gaaatcaata
     6901 aagtcgcatc cagcaagggc ttccatccta ttttcatttt tactgtgctc aatatacagc
     6961 ttcgccattt ttaagtacaa gctttgaatg tctcttatat cagttggtaa gtcagctcgt
     7021 tgcccagcaa aatttttatt atcggaagaa acggtttgga cactgaatct ttcgggtatt
     7081 ccttcttcta cgagaatttt agttatgatt ggattgttgg gttctggatt aacgacaaca
     7141 atgatttgac atacagaatc cttatccttt tcgtatctaa tgttatgaat ctgattaagt
     7201 attgattgtg tctgttgcgt aagtggtgtc gaacttatcg tttcaatgtt attaataaga
     7261 cttacaagtt gaaccacaac ttttgagtag tctgcatttg tatatgtggt gttcgtgtgg
     7321 catgcattaa tttgttggag ttgttgttgc gctaaatcaa attgttcagg gttgtatcct
     7381 tctccagaga atgtggaatt aatgggttgt tgttcttccc aattatcaca aggccacttc
     7441 ggtgtgtctg gcgttttaac attatcggta tttgttgtgc agttataaat atcgccgtga
     7501 tatgtattta ttatacctgg ggtaaagtca attgacataa cattccttat tcatcatctg
     7561 ttttatgcta atgtttttaa attggataat tagaacagga atggattttc ttctacacac
     7621 aataaatata atgcgttata tcttcctgat ataattacct ttaaattccc tttcatttcc
     7681 acccggtaat atttgtgtat aaaaaaaggg cagtgatagt atcgtaaagg aaaacgttaa
     7741 caactatcaa aaaacgctca ttttaaaaag ttattatgtt ctccgatagt gtgtgaagta
     7801 atatttttgt gatgttttag atgtgtaata atgaaccagg ttaaagtgta tttaaaaatg
     7861 tgttgtatta gcgactgaaa tctgcctttt aaaaaggagg agggtactga gacaaagagt
     7921 cattgcttct ccgataaaat gtcagagaag catgattacg tggttattgt ctgttgatga
     7981 cttgttgacc gagccgtcat cattctcaat gcattagtta aatcatttgt gatttttaag
     8041 gattatcctg tctatgattt tctacttaga aggatatggc actaactatg catcttatag
     8101 tttactgctg gcatctattt aaaatagcaa ttgagtcaaa tagcccatca agatagccat
     8161 acctaatata accgtcagga atgcagctat catcggcatt ctgaacattg atttaagtaa
     8221 tatcacttcc gtcaggcttg cgccggcact gccgatgatt aatgccatta atgctcccag
     8281 acccattcct tttgtcatca gaacagatgc cagagggata accgcctcag cccggatata
     8341 cagcggaata ccgacaacgg cgcttaatgg gatggcgaag ggattatctg atcctgcatg
     8401 agcggcaatc cacgctgagg gaataaagcc ataaataaaa gatcctatta atactcctaa
     8461 caaaagatat ggcagtacat ctttaaactg ttgcaacgca tctttggtgg ctagttttat
     8521 gggattaata cttactacag ctttggtact gcaacaggta actacggggt tctcaatggc
     8581 tttagccgct ggactacaac aactcacttt tatgggcgta tatgtcgttc ccggcgaagt
     8641 ttttgctgga gcacaacaat ttgctgatga gcttttactg gcaatgatat gacgttcaaa
     8701 ccccagagaa tccaggataa tactggcaag aacggagacg ccggctgcga taatcgcgta
     8761 caacaaggtg actttccaac caaaggtcat ccacattagt ccgacgataa tgggattaag
     8821 taatggtgaa acaaataaaa aagtgagagt cggaccaaac ccggctttcg ctgacaacaa
     8881 tccacgtaac atgggtattg tcgagcaact acagaacggg gtaacggctc ccaacagagc
     8941 agccaggaga tagcctctcc cttttctggc ccccatcatc tgctggattt tatggtctgg
     9001 caccttttgt cttatcaggc tgacaccggc actaatcact ataaacagca acgaaagctc
     9061 gacggcgaga aacacaaaca tctctgc
//
LOCUS       DAJTYH010000142         9061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTYH010000142 DAJTYH010000000
VERSION     DAJTYH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..251)
                     /locus_tag="OO609_003558"
     CDS             complement(<1..251)
                     /locus_tag="OO609_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR6634839.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEA"
     gene            complement(248..1126)
                     /gene="xdhB"
                     /locus_tag="OO609_003559"
     CDS             complement(248..1126)
                     /gene="xdhB"
                     /locus_tag="OO609_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6634840.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1137..3434)
                     /gene="xdhA"
                     /locus_tag="OO609_003560"
                     /pseudo
     CDS             complement(1137..3434)
                     /gene="xdhA"
                     /locus_tag="OO609_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            3849..4604
                     /locus_tag="OO609_003561"
     CDS             3849..4604
                     /locus_tag="OO609_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6634841.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4683..4756
                     /locus_tag="OO609_003562"
     tRNA            4683..4756
                     /locus_tag="OO609_003562"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4715..4717,aa:Gly,seq:ccc)
     gene            4891..6501
                     /locus_tag="OO609_003563"
     CDS             4891..6501
                     /locus_tag="OO609_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6634842.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6533..7140
                     /locus_tag="OO609_003564"
                     /pseudo
     CDS             6533..7140
                     /locus_tag="OO609_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8071..8817
                     /locus_tag="OO609_003565"
     CDS             8071..8817
                     /locus_tag="OO609_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6634843.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2314 a         2227 c         2323 g         2197 t
ORIGIN      
        1 ttcgcttcac cttccagcgt gcggatctct tttccttcag cccaggcggc aaggtataag
       61 caactgtcta ttgctgtgcc gtcgaccaac accgtacagg caccacattc acccacgcag
      121 cacccttgtt tgacacttag cagtccttgt tcgcggagta attccgagag cggcgtgcct
      181 ggtgcggcgt gaagctgaaa aggcatcccg ttaatggtgc attcggtggt aattgtttcg
      241 ctgtgattca ttgcaatttt ccccccgccg cggcgacggc ttcgctaatc acttttttgg
      301 tcatcgtctg gatgagatgc agacgaaact ctttactggc ccgccatgaa gaacgcgggg
      361 cgacatcttg caggacagat tcgctgatag cttccagcgt ttgcaggttt aatggcgcat
      421 tttgtgcagt ctgttcggca tgttggcagc gaatcggcgt tggcgcggca acaccaaatg
      481 ccaggcgtaa ttcgctgaaa ttgccgttat ccagtcggca atgtgcggcg cagccaatcg
      541 ttgaaatatc cattgcgtcg cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt
      601 tcggctgtgg cggaaaatga aaggcgacga ggatttcgtc atgctcaaga gacactttgc
      661 ccggcccggt gtgaaagcca ttaatcggga cgaaacgaac accgcgcggg gagtggatct
      721 ccagtttcgc gtcataaatt agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc
      781 aaatatttcc accgtaggta gcgacgttac ggatctgcgg tccagcaatg aacgtggccg
      841 cagcacataa cgccgggaga tgacgttgag ttataggatc ttctattagc tgggtaaatg
      901 tcgttgcaga gccgatacgt agcgagccat cttccgccag cgtaattccc cgcagctccg
      961 ccagattatg aatatcaaca atatggcgat aacggtcatt gtgatggtgg agctgaatca
     1021 gtacgtcagt gccaccggcg agcagtttgg cctgcgggtt gtcagccagc aggttgatgg
     1081 catcggcaag ggttgctgcg cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa
     1141 atcaatcctg ccagatggaa ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg
     1201 attgcaacac cggtagccat cttcaccgcg ttacgaatag cagcggcaac aggaattatg
     1261 ggtggctcac ccagtgactt atgtccgtat gcggattgcg gctcattgat ttcgacgaac
     1321 gcgctttcca gttgtggcag atccggcatg gtcggcattt tgtaatccag cagattgggg
     1381 ttacggacca cgccgctttt agcatcgatg atcatctctt caaatagcgc ccagccaatg
     1441 cccattccca ttccgccgtg tacctgacct tctgccagca gtggattaag aatatgccct
     1501 gaatcatgaa cgttgaggat gcggttgatg gtgactttgc acagcgcaat atcgaccgtc
     1561 agatcaacaa aggtacagcc aaacgccggt gggttagtgg tggttttgat ggagctttca
     1621 gcagagagct gcccgccgcg ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac
     1681 gacattaacg gctcttccgg tcgttcaacc agcacgatat ggccttttat cagggtcaaa
     1741 ttcatcgctg actgatgtag catgactgcg gcgtgagcga tgattttctc ttttaataat
     1801 agtgccgcac tgcgcagcgc aggcgcggca acatagctct ggcgtgaggc aaatgcgccg
     1861 ggatcgaacg gcgtaacgtc ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga
     1921 acccccacgg tttctgccac catttgcgag aagacggtgt cggcaccctg accgatttcc
     1981 gtcgcgccgc tttgcacgtt gatggttcca tcctgattca tcagaaggcg cgcgcctgct
     2041 atttctacgc cgacaggcca ggtgttagag gtgtagctaa aacaggcgac gccaacgccg
     2101 cggcgcaaat tgccttgctg gttctggcat tctgcacggc gtttttccca ttcaaagatt
     2161 ttccggcctt tttcaagaca ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc
     2221 ggattagcat ctccttcgcg ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct
     2281 aacgctgtcg cggcgtcatc aagcatagac tcaacggcaa atacgacttg tggcgcgcca
     2341 taaccacgca tcgcaccagc cgaggggagg ttggtatagc aggtctttga actgtaagcg
     2401 taggcacaac gaggataaag gtaagcgact ttattccccc ccgcagaagc gatggagtgc
     2461 ccgtgagatg cataagcgcc ggtgttagac agaacatcca gactataacc tttcaaggtt
     2521 ccgtcgcggt tcacgcccat ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg
     2581 aggaaacact cttcacggct aagggaaact ttcaccggaa tgccgccaag cttgctggtc
     2641 aggaatgccg ccattggctc ttccagtaca tcctgtttat taccaaaacc gccaccgaca
     2701 aacggtttga tgactcgtac gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg
     2761 cgaacaatgt gcgggatctg ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc
     2821 cacgccagcg atgttacgct ttccatgtga caatgttgaa taacgggagt ctgatagtgc
     2881 ccctgtacct ggtagtcggc ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg
     2941 ctttgtttca gtaaattgcc gccgttatgg attggtgcag cgtcttctgt cagcgccgct
     3001 tctggcgtgg tgataacggg taattcttgc cactcaatgc tgaccaattg cgccgctttt
     3061 tctgccgtga gttcatcgcg ggccacgacg atggcaacgg cgtcaccatg atgacgaaca
     3121 tggcgagtta gcagtgcgcg atcggcggta tcgcgcttgt tttcgtcaag tgtctaggca
     3181 tgcccagctg tagcgaatgg aatatcaggc acatcttccc aagtaaaaat cgccagtacg
     3241 cccggtaaac ttctggcttg ttcatcatta atacttacgg cataaccatg tgcgataggg
     3301 ctacgtacat atttcgcgta acacatgccc gccataacat aatcgtcagt atatcgtgcc
     3361 cgcccggtga ccttagcaat ggcatcgacg cgcatgcatg attcacccgt agcggttgct
     3421 tcccgcgctt ccatagaaat cccctcgatt tgaacatttg gttcttatgg caaagattta
     3481 gtctaagcag gcaagattta cgccagaaac gacgataagc gttaggggag gaaggaagtg
     3541 tgatgacgtt aaaaaaacac agatatgata cgaaacagga ctctgaatag ggacagtgat
     3601 acgtatcaat atgagtggcg ataaaatcaa tgtgataaat caggattatt acgcatcagg
     3661 atgggaaata gggtaaaaag tcaggaactt cgatacctct acagcgaata ttttgctgat
     3721 gatacttccc tttgccaaat gaaattaacc tctttctcta ttaccacgtt tttccagaag
     3781 caagagattg ggtatcaaga ggctggctgc tatgatatgg cgtcttgttt tttaagcgag
     3841 gaaagatttt gagtgcggga cgcctgaata aaaaatctct gggtatcgtg atgtttttat
     3901 cggttggact gctattggcg ggctgttcgg gtagcaaatc atccgataca ggaacgtatt
     3961 ccggctccgt ttacaccgtg aaacgggggg atacgctata tcgtatttcg cgcaccacgg
     4021 gaaccagcgt aaaagagctg gcgcgactga acggcatttc ccccccttac accattgaag
     4081 ttggtcagaa actaaaactg ggtggggcga aaagtagcag tagtacacgt aaatcaaccg
     4141 ccaaatcaac gactaaaacc gcatcggtta caccgtcatc agcggtaccg aaatcttcct
     4201 ggccgccagt agggcaacgt tgttggttat ggccaacgac agggaaagtt atcatgccgt
     4261 attcgacagc agatggcggc aataaaggga ttgatatctc agctccacgg ggtacaccta
     4321 tttacgccgc gggtgcagga aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta
     4381 atctcatcat gattaaacac agtgaagatt acattacggc ttacgcccat aatgacacga
     4441 tgctggtaaa taatgggcaa agcgtgaagg ctgggcaaaa aatcgccact atggggagca
     4501 cggatgcggc atctgttcgc ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc
     4561 cgctacgtta cttgccgccg cagggcagca agccaaaatg ctgatggcga attaatcagc
     4621 agtcagcagc gcggcacttg ctaaggagag cgtaaggttt ataatgcctt acgcatctcg
     4681 aagcgggcgt agttcaatgg tagaacgaga gcttcccaag ctctatacga gggttcgatt
     4741 cccttcgccc gctccatgac acttcttaaa gtctactaac ttactgaatc ctaagggata
     4801 cggcttaacg ccttccgata gttttactca ctgtaaccaa cactgtgact aactttgttg
     4861 aagtagtgag cagacctaag gtctcagtac atgctcaagt caagaaccta cctttatcag
     4921 cgtaatggtg tcttttacat ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg
     4981 cttccttctc acaaccgtta taaactagcg tcagtatctt tacggaccaa ggatagacgt
     5041 accgctatgg ctcactcacg gcacatcaag tcagcactta aagcaatcca tgcagacaac
     5101 cctaacgcct cttatgagga gctacgggag cacctgaaga ccattgtcga gtgggaactt
     5161 agtgtcagcc gtgatgacct gaacgacccg gagtcctacc agctttacgt tgaccagtac
     5221 gatgacatta agtccaacct tcgggaagct gtagcgaccg agcgtctgac tgtagaccag
     5281 catcgctata ttaatgacgt tatcggtgtg cttaaggcct gtcaggatag actcaatggc
     5341 gatagctctg gcctattgtc ttaccttgag cctgagactg gcagtctaag accgtccgtc
     5401 tccctatctg tattggcgga acctgaagtc cctgaaccta aagcactgac cctcgcgtct
     5461 ctcattgagc agtacgagca ggagaacgcc cagaactgga agccagcgac cctaagcgaa
     5521 aaccgagcgt cacactctac gctaatcgag atattcgact atctggatat tcaggatgtc
     5581 ggcaaggcga cccgtgcaga tatgctcaga gttcgtgaag tcctccagca gctaccgaag
     5641 aaccgcaagc aacgctttaa gtctatgccg ctgtctgact tgctgaaccg ggagtctaag
     5701 actgactgtc tggacgtcgt taccattaac aataaatatc ttatcaagat ggctgctgtg
     5761 tttaagtggg cggtacgcaa cgacttgata gctaagaacc tgaccgaagg tcttgagctg
     5821 aaagtccctc agcgtaaagc ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg
     5881 caactactgg tcgcagctaa agcgtactcg cagaagacct caggtaagcc ataccattat
     5941 tacgtcaccg ctctggctgc gattacaggt gcgagactta acgaggtggc ccaacttcag
     6001 gttaaagatg tcaggaccac tgaggctggg accgttttta tccatatcaa cgaagacgat
     6061 agcagcctgc cgggtaagag tatcaagaac gcccacagtg accgctgtgt cccgttggta
     6121 gatggtgctt atggttttgt attggctgac tttatgtcac tcgtggaaga ccgtagaaag
     6181 actgagggtg ataatgctat ggtgttcaat ggcctcaagc taatgaagaa cggctacggt
     6241 gagcaggtta gcaagtggtt taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc
     6301 ggcttagctt ttcactcgtt tagacataca gttgcgaccc agttaaaaca acatggggtc
     6361 gagttagcgt atgctcaggc gattatgggt catagctcag ggtctataac ttacgaccgt
     6421 tacgcgaaag aggtcgaggt ggaaacgctg aaagaaaaat tagcggagtc attgtcagta
     6481 aaaaaaatag acggaaagta atcgcatagg gatagcggaa tgtgaggacg taatggtcaa
     6541 agaattttta gttagcaaag aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac
     6601 ttgtttgggg ctggctggaa tggcttcgga agattggcaa cgtgagcatc attgccgcac
     6661 aggtataata tttactgctt ttgctattga ggcaatgttc atcttctata gaaaacaggt
     6721 tgaccccggc tacgataaga cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt
     6781 aaaattgtgc ggtataaaca attatatggg gacaaaacct taccaaatta tcaaagagtg
     6841 tcttgaggta agagacgcta ttgctcacgg cgattcgtat acttcgagct ttaatttctc
     6901 ggcggatcat ttggataacc aagacgatat tgcaaaagtg tcctggcggt aaactctgaa
     6961 caatttcggg gattaactgt tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt
     7021 gaccatgaga tttctgataa tggttacagg ccttctgaag gttaccttcc agcagccgag
     7081 cgcagaagtc tgatgttagc ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga
     7141 gcgataatac acctcactat agaggaatgg acccttaact atcactatag gggagagacc
     7201 ctaagtcccc accttaatag cagtcacgac ccatacacac attcattaag tctcaggctt
     7261 ccggtagcgg accgattgga aaccatccgc agacgcctag tgttaaatca ccttggcctg
     7321 agaccattcc ttttaacgta agctatcttc ccttttgaca ggcggggagc agtagaccac
     7381 atccgacttt aagttggctc tgtgataatc gctgcgcgga taactgaaga accaagacac
     7441 cattactgag gtctgccatc catttgtgga caggcggaaa ctcaaagagg ttagatcagt
     7501 ggtgtctgag gttggagctt tgagtcttga ccagcaggct ggaggagacc atgagtttat
     7561 cttgttatgg attgtgttat cacaattcca ataagaagaa ctttaagtcc agcctccact
     7621 cccgccaacc cgaagtaaaa ttatggtcgc tatgatgtgg tctccatgct tcgggtctta
     7681 ctggaagtac tgaacacttt cgcgtcaagt tctccgaagt tcattcctgt ttgactaaac
     7741 tatcactata gggaccatat ctcgcctctg acctcctgag agacggcaag cgagaaaccg
     7801 tggtccacgc tagcaagcta gccaccagcc taaggcaaga cagcctctgg tcagcctgtg
     7861 tgttctttgg tctcgtgact taagtctatc accacacacg ctcggacatg ctgtcgaacc
     7921 ttcatctcta tggtcatcgc agaccactaa ccccaattac ctcaagtcat tctgtttgcg
     7981 tcctgttgcc tgctggtctc cctttacgtg ggggcttgag taggtcagtg gggtgcgact
     8041 tcagggtgca aacaaaagga gtacacgtaa atgtcccaac aagaaatcag catcattaac
     8101 ctcgaccagc ttgtgtccat gacctcagtt gaaattgctg agctgacagg aaaagaacat
     8161 aaacacgttt tacgagatat tcgtaatatg gtcgaagagc ttaacggggc caaaactgag
     8221 cactgctcaa ctttgagcag cgagcttaac gggtccaaat ttgggctcgt tggcgaggaa
     8281 gtatacaagg acgcgaaagg tgagagccga acaatgtatc gtctcgaccg taagcatact
     8341 ttcattctgg tcgctggcta ttccgttcac ctccgcgcca agtgttacga ccatattcag
     8401 acgttagagc gtcgagtcct tcaactggaa gaccagaaga agcgagcagc cattcagtcc
     8461 gctaaccgcc gaggcgtgac ttggggcgac tactgcaaaa cctatggtct gcccgctcag
     8521 aagctaatga ccgcacttct ccagcatcgt ggcctctttc gtaagaaccc aatcagtaac
     8581 gagtggtctg taaatccgaa gtattccgac tgctttcgta tcattaagcc gtcagaccag
     8641 aagttctcag caggtgggta taacttccgg ttcaacgcta aggggctgga ggtcttcggt
     8701 aagcctgaga tggttgacaa gatgcgaggc atccttattg cattcactgg caccgaccag
     8761 cagaagcaag agcacctcct gaagctggca caatccggta aggtggaggg aatctaagat
     8821 gatgattgac ccgcgcacac ctgaggggcg catgactctc cgttaccgtg gctaccggac
     8881 tgaagtcctc ctcagagagc ttggtctaga cccggaagat gagacccgcc agcaccaatc
     8941 ccgagacgag ctaatagccc aactggtcgc tatgaaactg ccgcttaacc gttagtagct
     9001 cacctactca atacctaatt taaacacctt ttgagatgac cgatgcgggg agacccgtag
     9061 g
//
LOCUS       DAJTYH010000143         9049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTYH010000143 DAJTYH010000000
VERSION     DAJTYH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO609_003566"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO609_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6634844.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO609_003567"
     CDS             1828..3147
                     /locus_tag="OO609_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6634845.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO609_003568"
     CDS             3445..3486
                     /locus_tag="OO609_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634846.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO609_003569"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO609_003569"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6634847.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO609_003570"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO609_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO609_003571"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO609_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO609_003572"
     CDS             5815..6711
                     /locus_tag="OO609_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6634848.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO609_003573"
     CDS             6708..7682
                     /locus_tag="OO609_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6634849.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO609_003574"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO609_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..>9049)
                     /gene="osmC"
                     /locus_tag="OO609_003575"
     CDS             complement(8657..>9049)
                     /gene="osmC"
                     /locus_tag="OO609_003575"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6634850.1"
                     /translation="DIKRGKGTVSTESGVLNQQPYGFNTRFEGEKGTNPEELIGAAHA
                     ACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKSEVAVPGIDASTFD
                     GIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2195 a         2219 c         2280 g         2355 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatc
//
LOCUS       DAJTYH010000144         8881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTYH010000144 DAJTYH010000000
VERSION     DAJTYH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..1325)
                     /gene="trkA"
                     /locus_tag="OO609_003576"
     CDS             complement(<1..1325)
                     /gene="trkA"
                     /locus_tag="OO609_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6634851.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDK"
     gene            complement(1347..2636)
                     /gene="rsmB"
                     /locus_tag="OO609_003577"
     CDS             complement(1347..2636)
                     /gene="rsmB"
                     /locus_tag="OO609_003577"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6634852.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2682..3629)
                     /gene="fmt"
                     /locus_tag="OO609_003578"
     CDS             complement(2682..3629)
                     /gene="fmt"
                     /locus_tag="OO609_003578"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6634853.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3644..4153)
                     /gene="def"
                     /locus_tag="OO609_003579"
     CDS             complement(3644..4153)
                     /gene="def"
                     /locus_tag="OO609_003579"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6634854.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4318..5442
                     /gene="dprA"
                     /locus_tag="OO609_003580"
     CDS             4318..5442
                     /gene="dprA"
                     /locus_tag="OO609_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6634855.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5414..5887
                     /locus_tag="OO609_003581"
     CDS             5414..5887
                     /locus_tag="OO609_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6634856.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5916..6458
                     /locus_tag="OO609_003582"
     CDS             5916..6458
                     /locus_tag="OO609_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6634857.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6463..7035
                     /gene="tsaC"
                     /locus_tag="OO609_003583"
     CDS             6463..7035
                     /gene="tsaC"
                     /locus_tag="OO609_003583"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6634858.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7040..7858
                     /gene="aroE"
                     /locus_tag="OO609_003584"
     CDS             7040..7858
                     /gene="aroE"
                     /locus_tag="OO609_003584"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6634859.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7855..8112
                     /locus_tag="OO609_003585"
     CDS             7855..8112
                     /locus_tag="OO609_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6634860.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8088..8642)
                     /locus_tag="OO609_003586"
     CDS             complement(8088..8642)
                     /locus_tag="OO609_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6634861.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2206 a         2168 c         2222 g         2285 t
ORIGIN      
        1 tttttatctg tgaggaacat aattacgtga tcgccttgct caatgcgcag attgtcattg
       61 gcaatcatca cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg
      121 atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct
      181 tcaatagctt ctgctacgcc gcggcgcaat gaggaaacac caacaatatc tgcttttcgc
      241 acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaattgcaat atcgatcacg
      301 ctcccctgaa ccagatccac ataagcgcga cgctggatca ataccatcac ctttttcgca
      361 cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt ggtgacggca
      421 ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca
      481 ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta
      541 cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata
      601 ttaccgccac caaccagcat gatccgttta tacggttttt ccagtcgctg taattcactc
      661 atcaccgcgc ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttcaaca
      721 atggtcgaac cttgtggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta
      781 tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcat taccaatcag cgggccgcca
      841 taataggctt taactacagc gaggctgact ttaccctcag cgaagttcac cacctgcaat
      901 gcgcctggat actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc
      961 agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg tacgtagtct
     1021 ggtgagcgga tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag
     1081 gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc gtcgtcggca
     1141 cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgactcg caggtcaaat
     1201 ttatcctgca aggtccgcag acgctcaccg ttggtatcga caacagtaat atcgttgttc
     1261 tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt
     1321 ttcatcagtc gcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg
     1381 tcgccctctt cggcaccagg ctgattttgt ttacccggtt gctctggtgt tcctgtttcg
     1441 caaagttcgg catcagcggt acgttgcaaa aaggctttaa tctgcaggct attctcttcc
     1501 ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg cggccaaatg
     1561 gcgtcgagaa tttcagactg caattgcgcg agttccggga tatcgcgatc gcgacgtaac
     1621 catttaatat ctggatggcg acgaatcacc cccgttgctg aacaaggcgc atctaataaa
     1681 atgcgatcaa actgttgctc gccacaccat tgggaagggt aacggccatc accttgtttc
     1741 acggttgcct tcataccaag gcgttttaaa ttgtcgtaaa tgcgagagag gcgctgttcg
     1801 tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg cgttgtttta
     1861 ccgccggggg cggcacaaag atccaagatg tgttcaccgt tttgtggcgc aagccaggtc
     1921 atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa accaggtagc
     1981 gcatgaacag gtgcaggtgt ttccagccgt acagcatcag ggtaatccgc atgcgggaaa
     2041 cctttcattc ctgcttcatc cagcaatgca agccagctgt cgcgggaatg atgcgtacga
     2101 ttaatacgca gccacattgg cggacgctgg ttattggctt cggcgatgga ttgccactgc
     2161 tctggatacg ctttttgcag acgcttcagc aaccaggaag gatgcagata acgtgcatca
     2221 cgggtattaa actcggctaa taactcttct tgccgacgct ggaactggcg taatacaccg
     2281 ttaatcaacc ctttaagttg cggacgctta attgcgatag cgccttcaac cgtttcagcc
     2341 agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac cataatcagg
     2401 taatgcacag tgcgctgttt gccggtcatc ggacgggcca ttaacttatt aatcagccag
     2461 tctaactgcg aaagcgtacg cagtacgcca aagcacaact cttgaagaag tgctttgtct
     2521 ttatcggaaa ctttttgctg gagcggtggc agaatgttgc ttaatgattg cccttgctcg
     2581 acgacttgtt caacggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt
     2641 ataaaaatgc tcggcaagac cgggcttaga agagtggact atcagaccag acggttgccc
     2701 ggaacaaacc attcccgacg agagttcagg aggtcttgcg cgctcatcgc tttcttaccc
     2761 gcaggttgta acgagagcag gttcaggatg ccatcaccag tcgcaacctg aatgccttgt
     2821 ttgttggctt caaggatcgt tcctggtgca gcgttggttg ccgtatcaat gaccgacgct
     2881 ttccagactt taaccggctg tccttcaatt tccagccagc tcattggcca tgggttgaaa
     2941 gcgcgaatgc agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg cgcttcttct
     3001 ttactcaact tctcggcgta agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg
     3061 ccgtctgcca attgtttcaa cgtggtgata agcccttgtg ggccaagctc tgccagcttg
     3121 tcgtacaacg taccactggt atcttctgca gtaatcgggc aggagagctt atagagcatg
     3181 tcaccggtgt ctaaaccaac atccatttgc ataatggtta caccagtttc tgcatcaccc
     3241 gcccatagtg agcgttggat tggtgcagca ccgcgccagc gtggcagcag tgaaccatga
     3301 atgttgatac agccaagacg cggcatctcc agcacagctt taggcagaat taaaccatag
     3361 gcgacgacga ccataacatc agcctgcaaa tcggcgacca gttgctggtt ttcttgtgga
     3421 cgcagggaaa caggttgaaa aacgggcaga ccttcttcct cagccagaac tttaatcggg
     3481 ctgggcatca gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg
     3541 acgttatgac cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc
     3601 gcaaaaataa tacgtagtga ttctgacacg ttagttctta tccttaagcc cgggctttca
     3661 gacgatccag tttttcaact ttctgacgaa ttcgttgttg tttcagcggt gacagataat
     3721 ccataaacag tttgccaacc aggtgatcca tctcatgctg aatacagata gctaacagac
     3781 cctctgcttc cagttcaaat ggtttaccgt cgcggtcaag ggcgcgaatt ttaactttct
     3841 ctgcgcgcgg cactaaagca cgttgttcag ggatcgacag gcaaccttct tcaatgcctg
     3901 tttcgccgct tttttctaaa agctccggat tgattaacac cagccgttcg tcacggtttt
     3961 ccgaaacatc aataacaatg atacgttgat ggatatcaac ctgggttgcc gccaggccaa
     4021 taccttcttc tgcgtacatc gtctcgaaca tatcatcgac gatacgctga atttctgcat
     4081 tcacttcttc taccggttta gcaactttgc gaagccgctc gtccggaata tgtaacactt
     4141 gcaaaactga cataaatctc cagagatgtg ttcaggagtt agaaagatta tttcttctat
     4201 tctagacaaa tccccctctg attgacagca tcacttgatt gacagcatca cttgattgac
     4261 agcatcactg accaatcgca aagattgcta aggcttgctt atggcaggga gataaggatg
     4321 gtcgatatag aaatttggct gcgtttaatg agcatcagca gcttgtacgg cgatgatatg
     4381 gtccgtatcg ctcactggct ggcaaaacag tcgcatattg atgcggttgt attgcagcaa
     4441 acagggctta cattgcggca ggcacaacgc tttctttcat ttccacgaaa gagtatcgaa
     4501 agctcacttt gttggttgga gcaacccaac catcatttaa tccctgcgga cagcgaattt
     4561 tatcctcctc aacttcaggc gacgacagat taccccggcg cactgtttgt tgaaggagaa
     4621 ctgcacgcgc tgcattcatt tcagcttgcc gtagtgggga gtcgggcgca ttcatggtat
     4681 ggcgagcgat gggggcgatt attttgcgaa actctggcga cgtgtggagt gacaattacg
     4741 agtggactgg cgcgtggaat cgatggtgtg gtgcataaag cggccttaca ggtaaatggc
     4801 gtcagcattg ctgtattggg gaatggactt aataccattc atccgcgtcg ccatgcccga
     4861 ctggctgcca gtctgtttga acagggtggc gctctcgtct cggaatttcc cctcgatgtt
     4921 ccaccccttg cttacaattt cccacgaaga aatcgcatta tcagtggtct aagtaaaggt
     4981 gtactggtgg tggaagcggc tttgcgcagt ggttcgctgg tgacagcacg ttgtgcgctt
     5041 gagcaggggc gtgaagtttt tgccttgcca ggtccaatag ggaatccggg aagcgaaggg
     5101 cctcactggt taataaaaca aggtgcgatt cttgtgacgg aaccggaaga aattctggaa
     5161 aacttgcaat ttggattgca ctggttgcca gacgcccctg aaaattcatt ttattcacca
     5221 gatcagcaag acgtggcatt gccatttcct gagctcctgg ctaacgtagg agatgaggta
     5281 acacctgttg acgtcgtcgc tgaacgtgcc ggccaacctg tgccagaggt agttactcaa
     5341 ctactcgaac tggagttagc aggatggatc gcagctgtac ccggcggcta tgtccgattg
     5401 aggagggcat gccatgttcg acgtactaat gtatttgttt gaaacctata ttcacactga
     5461 agctgagttg cgtgtggatc aagacaaact tgaacaggat cttaccgacg caggttttga
     5521 gcgagaagat atctacaatg ccctgctatg gctggaaaaa cttgctgatt atcaggaagg
     5581 gttggcagaa ccgatgcaac tggcctcgga tcctctctcc atgcgtattt atacaccgga
     5641 agagtgtgaa cgactggatg ccagctgccg tgggtttctg cttttccttg agcagattca
     5701 ggtgctcaac cttgaaactc gtgaaatggt gatagagcga gtgctcgcgc tggataacgc
     5761 agagttcgaa ctggatgatc tgaaatgggt gatcctgatg gtgttgttca acatcccggg
     5821 ctgcgaaaat gcgtaccagc aaatggaaga attactcttt gaagtgaatg aaggtatgct
     5881 gcattaatca tttctttaat tcagcataag ttgttatggc aaaatcagca ctgttcacgg
     5941 tgcgtaataa tgagtcctgc ccaaagtgcg gggctgaact ggttattcga tccgggaaac
     6001 acggtccgtt tcttggatgc tcacagtatc cggcgtgtga ctacgtccgt cctctgaaat
     6061 cttcagcgga tggacatatt gtcaaagttc tggaggggca ggtttgcccc gtatgtggcg
     6121 caaatctggt attacgccag ggacgctttg gtatgtttat tggttgcagt aactaccctg
     6181 aatgcgaaca taccgaactt atcgataaac cggacgaaac agcaattaca tgcccccaat
     6241 gtcggacggg ccatctggtc cagcgccgct cccgttatgg caaaacattt cactcctgtg
     6301 atcgctaccc ggagtgtcaa tttgccatta acttcaaacc aatagctgga gaatgccctg
     6361 agtgtcatta tccgctactc atcgaaaaga aaaccgcgca gggtgtaaaa cacttttgtg
     6421 ccagtaaaca atgtggaaag ccggtttcgg cggaataata acgtgaataa taacctgcaa
     6481 ggagacgcta tcgcagctgc gatagatgtc ctcaatgaaa aacgtgtcat cgcctatcca
     6541 acggaagccg ttttcggtgt cgggtgcgat cctgatagcg aaacagcagt gatgagactg
     6601 ttggagttaa aacagcgtcc ggttgataag gggctgattt taatcgcggc aaattacgag
     6661 cagcttaaac cctatattga tgacaccatg ttgactgacg tgcagcgtga aaccattttt
     6721 tcctgctggc caggtcctgt cacctttgtc tttcccgcgc ctgcgacaac accgcgctgg
     6781 ttgacgggcc gctttgattc gcttgctgta cgagtcaccg accatccgtt ggtggttgct
     6841 ttgtgccagg cttatggtaa accgctggtt tctaccagtg ccaacttgag tggattgcca
     6901 ccttgtcgaa cagtagacga agttcgcgca caatttggcg cggcgttccc ggttgtgcct
     6961 ggtgaaacgg gggggcgttt aaatccttca gaaatccgcg atgccctgac gggtgaactg
     7021 tttcgacagg ggtaacataa tggaaaccta tgctgttttt ggtaatccga tagcccacag
     7081 caaatcgcca ttcattcatc agcaatttgc tcagcaactg aatattgaac atccctatgg
     7141 gcgcgtgttg gcacccatca atgatttcat caacacactg aacgctttct ttcgtgctgg
     7201 tggtaaaggt gcgaatgtga cggtgccttt taaagaagag gcttttgcca gagcggatga
     7261 gcttactgaa cgggcagcgt tggctggtgc agttaatacc ctcaagcggt tagaagatgg
     7321 acgcctgctg ggtgacaata ccgatggtgt aggcttgtta agcgatctgg aacgtctgtc
     7381 ttttatccgc cctggtttac gtattctgct tatcggcgct ggtggagcat ctcgcggcgt
     7441 actactgcca cttctttccc tggactgtgc ggtgacaatc actaatcgga cggtatcccg
     7501 cgcggaagag ttggctaaat tgtttgcgca cactggcagt attcaggcgt tgggtatgga
     7561 cgaactggaa ggtcatgagt ttgatctcat tattaatgca acatccagtg gcatcagtgg
     7621 tgatattccg gcgatcccgt catcgctcat tcatccaggc atttattgct atgacatgtt
     7681 ctatcagaaa ggaaaaactc cttttctggc atggtgtgag cagcgaggct caaagcgtaa
     7741 tgctgatggt ttaggaatgc tggtggcaca ggcggctcat gcctttcttc tctggcacgg
     7801 tgttctgcct gacgtagaac cagttataaa gcaattgcag gaggaattgt ccgcgtgaat
     7861 caggccatcc agtttccgga cagggaagag tgggacgaga ataaaaaatg tgtatgtttt
     7921 cccgctctcg tgaatggtat gcaactgaca tgcgcgatct ctggcgagag tctggcgtat
     7981 cgctttactg gagatacgcc agaacagtgg ttagcgagtt ttcgtcagca tcgctgggac
     8041 ctggaagaag aagcggaaaa cttaattcag gaacaaagtg aagatgatca aggctgggtc
     8101 tggttaccct gatccagata ttcgtccttc catttcacgt aattattcgc ggaatagcgt
     8161 aacccagcct tctcttcatc acttaacggg cggatctgtt tgacggggct accgagatac
     8221 agatatccgc tctccagccg tttattttgt gggaccagac tacccgcacc aatcatcaca
     8281 tcatcttcta ctattgcgcc atcaagtaaa attgagccca tcccaaccaa aactcgattg
     8341 ccaatggtgc agccgtggag catcaccttg tgaccaacag tgacatcttc gccaatggtt
     8401 aatgggttgc catctgggtt gtacgaggat ttatgagtga catgcaacat actgccatcc
     8461 tggatattgg tgcgtgctcc gatctgtaca taatgtacat ctccacgaat cacaacgagc
     8521 ggccagatcc ccacatcatc agccagacga acgtcaccaa tcacgacact gctatcgtcg
     8581 atcattacgc gctgaccgat ttgtggaaaa agatcgcggt atgggcgtaa aacatcagac
     8641 atacttacct cagcaataaa tgatttacta atgactttgg gggcattatt ggccttgtgc
     8701 aagtctttta gtatgcaaaa aagcaccgtt ttgtgtgcga ttgcagcaaa aagggtgaaa
     8761 aaacaacaaa cagaaaagaa gatcaaaaaa atacttgtgc aaaatattgg gatccctata
     8821 atgcgcctcc gttgagacga caacgtgaaa cacttcacag gatggtcgga acaacgaaga
     8881 g
//
LOCUS       DAJTYH010000145         8879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTYH010000145 DAJTYH010000000
VERSION     DAJTYH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..723
                     /locus_tag="OO609_003587"
     CDS             <1..723
                     /locus_tag="OO609_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634862.1"
                     /translation="ACKELPQSVEYVPLACEAHSHRSVALAKTVAAPIMATGVGLALM
                     YATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHE
                     RNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRL
                     ADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNKFNVGLQGNINTDVLQ
                     DSFFKNVDFIHEYNSYTVGMGV"
     gene            1402..1620
                     /locus_tag="OO609_003588"
     CDS             1402..1620
                     /locus_tag="OO609_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6634863.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            1838..2080
                     /locus_tag="OO609_003589"
     CDS             1838..2080
                     /locus_tag="OO609_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634864.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2410..3339)
                     /gene="fdhE"
                     /locus_tag="OO609_003590"
     CDS             complement(2410..3339)
                     /gene="fdhE"
                     /locus_tag="OO609_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6634865.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3336..3971)
                     /gene="fdoI"
                     /locus_tag="OO609_003591"
     CDS             complement(3336..3971)
                     /gene="fdoI"
                     /locus_tag="OO609_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6634866.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(3968..4870)
                     /gene="fdoH"
                     /locus_tag="OO609_003592"
     CDS             complement(3968..4870)
                     /gene="fdoH"
                     /locus_tag="OO609_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6634867.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(4883..7933)
                     /gene="fdnG"
                     /locus_tag="OO609_003593"
     CDS             complement(4883..7933)
                     /gene="fdnG"
                     /locus_tag="OO609_003593"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7346..7348),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6634868.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8127..>8879
                     /gene="fdhD"
                     /locus_tag="OO609_003594"
     CDS             8127..>8879
                     /gene="fdhD"
                     /locus_tag="OO609_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6634869.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAE"
BASE COUNT         2126 a         2222 c         2328 g         2203 t
ORIGIN      
        1 gcatgtaagg aattaccgca gtcagtggaa tatgttcctt tagcctgtga agcgcatagc
       61 catcgcagcg tcgcccttgc caaaaccgtg gctgcgccca tcatggcgac aggtgtgggc
      121 ttggcattaa tgtatgcaac atcatcagga ccgtattatg cgcaaagtct ttctggttcg
      181 ccggatatcg agatgataaa aaatcgcatg gctgaattgt ttaaccagtc ggatatttct
      241 gtgcgcaaca aatatagccc ggcttttgat aaatggaatg aactggtaga taaattaaat
      301 cacgaaagaa atacagttcc gtttgcgtgt ttgaccacca ttttagcgac ggcgataaat
      361 gaaactcttg agggggatac gaatgttgcc gttgtgatgg ggtgtaaatc tgcgaaagat
      421 cgcacgatat ccattgtcct tggcaactcg atgttgcaaa cactccttga aaaacgcctc
      481 gccgatagtg gagaaataga aaatcttttt gatcaacagg gatattttag ttgcgattca
      541 ttaacacccg aagaattaat gatgttaaaa gacctttttg atattcgagt tcttcatgtc
      601 tctaacaaat tcaatgttgg attgcaaggt aatatcaaca ctgatgtttt acaagattcg
      661 ttttttaaaa atgtcgattt cattcatgaa tataactcgt ataccgtagg gatgggagtc
      721 tgacgagtaa aactcattag gattatcaga attcggacaa tgcattgcaa aaatgacata
      781 caaagagaga tgtgtgcagg tacttagccg cgaggggcat ggcgaagcca tacagaaagt
      841 accagtattc agaactccag tgaaagcggc atagtggccg cctgctcagg agttttgcta
      901 tgacccgtaa tcgtgctgaa cgtcgccacc ctatgatgcg attaaaaaaa ctcatcgtcg
      961 cgctgatgat tgacgtcaat gctatcggat gatgccgggg gtgttccgga aggaattcct
     1021 tgtttcccga actgttacca acaaaattat aacgatgagc aagtaaagac cggttggtgg
     1081 cagtactgac cggtctgtga tgaaagatta atttcacctt aaatcctggt gctgttagga
     1141 tgctgacgtt tttcggctac accattcaga ttatgtaaat aaatatccct gaatataata
     1201 tattcatagc acccataact catcctgggc gataactaaa ccatttttga gtaataaatg
     1261 aaacaactgc aacgctctta ttatttctgg aaaacgcctc gcaaaaacaa aatgacatgc
     1321 aaaaatccgc atttttttaa atgcttttcc ggcgtaattt tcgcacatac attaatgagt
     1381 aatatatgta aggtggtggt aatggcgatg aatacggttt ttcttcattt atcagaagag
     1441 gcaattaaac ggctgaacaa gcttcggggc tggcgtaagg tttcacgatc tgcaatttta
     1501 cgcgaagcgg ttgagcaata tctggagcgg cagcaatttc cggtgcgtaa agcaaaggga
     1561 ggcaggcaaa agggggaggt agttggtgtt gatgatcagt gtaaggagca taaggaatga
     1621 aaaaaatttt tcattcttga atataaaaaa cagatgccct tattctggta ttaatacaag
     1681 gctgttttac ttgaacttat aataactgca actgttacat cgtatctgga aaacgcctcg
     1741 caaaaataaa aaatgatgcg taaatgagct ttctttaaag attcggcaag ggtacttttc
     1801 tctccataga ttcaaaaatg atattggcga gatatttatg aactctctgg caggtataga
     1861 tatgggcaga attttactcg atttatcgaa tgaggtgatt aagcaacttg atgatctcga
     1921 ggtgcagcgt aatcttcctc gtgcggacct attaagggaa gcggtagatc aatacctgat
     1981 aaatcaatcg caaacagcaa gaaccagtgt tcctggcatc tggcaagggt gtgaggaaga
     2041 tggtgtcgaa tatcagcgta agctgcgcga ggaatggtaa tgggaaaaaa tgatgtcaat
     2101 cagattgctg acaacgtgcg cgttgttcat gccggatgcg gcgtgaacgc cttatccggc
     2161 ctacaaagtc atgcaaattc aatatattga agtttattgg taggcctgat aagcgcagcg
     2221 catcaggcaa tcatacgctt gtcatcagtg acatacccat aaccttatcg atgtgttgga
     2281 gggaagaacg aggcaaacag acaattgcgg catgatcttg aatgggtaac gcggaatacg
     2341 agagatggtg tcgtcacacc ctatgagctg taatccgggc gacctgcgcc acccggaaaa
     2401 cgtcagagat tactctcctt cacccggaaa caggaacggg ttaatggaac tacgggcgta
     2461 gccttcttgc tccattcgcg cgtccagtac cagagaggcg aggtcatctg ccacggcttc
     2521 aactttcggt tctttttcct gataaagaat cttcaggtaa gtgccgcagt catcgcagct
     2581 ttcggcttta atcgcggcct gttcgtcatc cagcgaccag taatgcagtt tgccgctctg
     2641 ctcgcagttg ctgcatttta cgcgcactac gtgccattcg gtttcacaca ggttgcagtg
     2701 caggtaacgc agaccctgag tggtgccaat ttgcaccatg ctggacaccg gcatagagcc
     2761 acataccggg caatattgac gttgttcgcc gtattcagcg cgggctttgc cggggatcag
     2821 attggccatc tgcgcccagt agagcgacag cgcagcccag ataaacggcg ctttatcgct
     2881 gctgacggac gagaaatcag aggcaaacag cgcgctggcc atatcttcca gctcctgagt
     2941 cgatgccttc tccagattct caatcactgc cagcgccggg ccgctcattt caggtttcag
     3001 ctcagcaatt agcgccatca gcagcttttg ccagtgctta tcacgcggca gaacgtgaat
     3061 atccagcggg ggcttgccct gtgcgctggc ttctttaatg cgcgcggtca gatccatctc
     3121 cagcggatgg tcgtacagca ccactttctg ggcgtgggcg ataagcgcag caaagcgcag
     3181 gtaatcaccc agcggattat tttctgccag ctcgcgcaga cgctcggcgc ggcggttgta
     3241 taaattcttg agccgaggga acaataacgg cggaatcata tccgccgtac gtttctcgct
     3301 cgaacccagc tcatcttgcg ggattatgcg aatactcatt cagctttctt ttccgttgtc
     3361 ttgcggacct cacggtacca gcgcgggtga tgtttcttcg cccaggcgct ggtaacccat
     3421 ccttccacca tggcggtaat cgtgcctttc acccaaaggg cggcgtagat atgcaccatg
     3481 ataaccacaa ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa tcggatcacc
     3541 gggattgaga aagcaggcgc aaaataagga cgccagataa tcacgccgct caccagcagc
     3601 agaaccagga aaataatcgc cgcccagaaa acgcatttct gaccgaagtt ataacgcccg
     3661 gtgtcaccta cttcctcgtt gacgacgatc ttacgaatat tcttcgccca aaagatatca
     3721 tcccgattga ttaggttttg atgccagtaa cggaaaaaca tgatgatgaa cgaggcaaac
     3781 ataaccacgc cgacaaacgg gtgcagaatt cgcgccagct gcggtgtgcc catgatttgc
     3841 atcaaccagt tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc caggatgaag
     3901 cagaaggcgg tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac gatggtgtca
     3961 cgtcgtttca tttgcgctcc tcgtctttct cttcgtgcag attattctct tcctcatccg
     4021 cacggttcgg accgacaccc acgtagtgga agatactggc cgcaaaggta gccgcaaagc
     4081 caacagccgc gagcggtttc cagatgcctt tccagaattt cacggtttcg ctgatttccg
     4141 ggttctccgg caagccatga tacagatttg gcttgtcagc atggtgcagc acgtacatga
     4201 cgtgtgtacc accgacgcct gccggatcgt acagacccgc attgtcgtaa ccgcgggttt
     4261 tcagctcagc aacgcgctcg ctcgccagcg ttttcatcga ctctttcgtg ccgaagtgaa
     4321 tcgcgcccgt tgggcaggtc ttcacgcagg ctggttcttg cccaaccacc acgcggtcaa
     4381 cgcacagcgt acatttgtag acgcggttgt cttccgggtt gaggcgcgga atgtcgaacg
     4441 gacagcccgc aatgcaataa ccgcagccaa tgcactgctc ggactggaag tcgacgatac
     4501 cgttggcata ctgaataatt gcaccttccg ccgggcacgc tttcaggcag cctggatcgg
     4561 aacagtgcat acagccgtct ttgcggatca gccattccag tttgtcgttc tgctccactt
     4621 ccgagaagcg cattaccgtc cacgatttgg cgcttaaatc attggggttg tcgtacaccc
     4681 caatgttatt gccgacggta tcgcgaatgt cgttccactc tgaacacgcc acctgacagg
     4741 ctttacagcc gatacaggtg gtaacgtcga tgagtttcgc cacttcttcc tggaagtccc
     4801 gcgcctgagg cgcgggggtc agaccgttag tcgcggaacg acgaatgata tcttgagatt
     4861 gataagccat atgtcgtctc cgttacacct tttccacatt cacaaggaag gacttaaact
     4921 ccggcgtctg cgtgttcgca tcaccaacga atggcgttaa agtattggca ataaagcctt
     4981 ttttagctac gccttcatag ccccagtgaa tcggaatacc gatggtatcg atatctttgc
     5041 cgttcgcttt cagcgtgcga atacgtttgg tcaccaccgc tttggctttg atatagccac
     5101 ggttggagga gactttcacg gtatcgccct gggcaatgcc aagtttattc gccagcgact
     5161 ccccgatttc cacaaactgc tctggctgca aaatcgcgtt caacagcgcg tgtttggtcc
     5221 agtagtggaa gtgctcggtc agacgatagg tggttccgac atacgggaac ttatcggctt
     5281 tacccaatgc ttcggcgtcg tctttaaaga tacgtgcagc tgggttcgag ataacgtttg
     5341 gatgcagcgg gttagttccc agcggcgttt caaacggctc gtagtgttcc gggaacggac
     5401 cttccgccat cttatcgagg gcaaacagac gccccatgcc ttcctgctgc atgataaacg
     5461 gcccgacgcc gctacccgga ggcgctgcgc tgtagtccgg aatatcccag ccggtccact
     5521 tagtgccgtc ccatttcagc aactgacgct tcggatccca cgggttaccc tgcggatctg
     5581 cggaggcgcg gttatacaga atgcggcggt taagcggcca tgcccatgcc cagcccagcg
     5641 tgttaccgag gccagacgga tcggcgttat cacgacgcgc catctggttg ccttccggcg
     5701 tccagctacc ggcgaaaatc cagcagccac aggaggttgt accgtcatcg cgcaattggg
     5761 cgaacgagct aagttgttgg cctttcttga cgataaccgc accggttgcc ggatcggtaa
     5821 tatcggccag cgccttaccg ttgctctcca tcgctacttc ttccgacttc ggctcatgcg
     5881 gaatggcgta gttccaggtc atgttcagca cctgatccgg gttcgcgcca ccctgttcgg
     5941 catacatctt gcgcaagcgc aggaagatac cggagaggat ctcaccgtca gtcagcgcaa
     6001 tccccggggc gtccgcacct ttccagtgcc actgcaacca gcgacctgag ttaacgattg
     6061 aaccgttctc ttctgcgaag caggtcgatg gcagacggaa cacttcggtc tggatcttcg
     6121 acgagtcaac ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt tcagtgttca
     6181 gtgggtcgat ggtgacgagg aacttcagtt tcgacagaca accgatcact ttgtttttgt
     6241 tcgggaatga ggcaacaggg ttaaagccct ggcagatata gccattgacc ttgccctctt
     6301 tcatcatctc gaagtattgc aggacgtcgt agcctttatc ccacttcggc aaccagtcaa
     6361 agccccagct attttccgcc gtcgctttat caccaaagaa ggccttcatc atagagacga
     6421 agaatttcgg gtagttgccc cagtagttta cctggccttc cagcagcggt tttggcgtgt
     6481 tggcggtaag gtaggtttgc agatcggtct gcttctcgct tggcagcgtc atgtaacctg
     6541 gcagactctg cgacagtagc cccaggtccg tcagcccctg aatattggag tgaccgcgca
     6601 gggcgttaac gccgccgccg gccatcccca tattgccgag cagcagctgg atcatcgcca
     6661 tcgtacgaat gttttgcgca ccaacggagt gttgcgtcca gccgagggca tacaggaacg
     6721 aggcagtttt atcgtgagca ctggtttctg cgatgtattc gcagactttc aggaacgcgt
     6781 cttttggcgt accacagatg ttttcaacca catctggcgt gtagcgggaa acgtgctgtt
     6841 tcagcaagtt ccacacgcag cgcggatgtt gcagcgtggt atcgcgtttg gcgaagccgt
     6901 tttcgtccag ttcataagtc caggaggatt tatcgtactt gcgtttttcc gcgtcgtagc
     6961 cggtgaacag gccatcttca aagccgtaat cctcacgcac gatcaggctg gcgttggtat
     7021 aggcttcggt gtattcgtgg ttgaattttt cattgttcag caggtacagc aatacgcctg
     7081 acaggaaagc aatgtcagta ccggaacgaa taggggcata gtagtcagct accgccgccg
     7141 tacgcgtaaa gcgaggatcg atcacaatca gcttcgcgcc gttgtgaatt ttggcttcca
     7201 tcgcccagcg gaacccgacc gggtgagctt cagcggcgtt accgcccatc accacgacga
     7261 ggttggcgtt cttgatgtcg acccagtggt tggtcatcgc accgcgacca aatgttggag
     7321 caagacttgc taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc gcgagcatac
     7381 ccagcgcgcg ggagaatttt tgcgttaaat agccggtttc gttactcgac gcggaagcac
     7441 acagcatccc ggtggagagc cagcggttaa cagtcacgcc ttcggcgttt tgcgcaatgt
     7501 agttagcatc gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc tcttcccaac
     7561 tgatttgctg ccatttatca gaacctggcg cacggtattc cggaaacttc agacggcttt
     7621 cggagtggat gaaatccacc aggccagcgc ctttcggaca aagtgcaccg cggttgaccg
     7681 ggtgatccgg atcgccttcg atatggaaga tagatgcttt ggcgtttttt gctccgtcac
     7741 cgaggctgta catcaacagc ccacagccta cggaacaata ggtgcaggta ttacgggttt
     7801 cgcgggtgcg cagcagttta tactgccggg tttccgcgag tgctacgctg ggtgcaaaac
     7861 ccagtgccgc tgccgtggtg cctgccatac cgccagcgca gatcttaaag aactgccttc
     7921 tgctgacctg catggattgc tccttgtttc gacattgtca cgtcccattt acattcgctt
     7981 gctgcgtgtg cagggagtgg gagttatttt tctttgcgga aggggccgca aaggtccaga
     8041 attggctcaa tttccctcca tctaggaagg gtttgtaaca gaataccata atgttggtgt
     8101 gtatgttctt atctggttaa gagaaagtga aaaaaacaca gcgaaaagaa atcgaaaatg
     8161 tgacaaatat cacaggtgtt cgtcaaattg agttatggcg gcgtgatgat ttacaacacc
     8221 cacggttaga tgaggtcgcg gaagaagttc ccgttgcgct ggtctacaac ggcatttcgc
     8281 atgtggtgat gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt ttttcgcttt
     8341 ccgaggggat aatagaaagt ccgcgcgata tcttcggcat ggatgtcgtt ccttcctgta
     8401 atggtcttga agtacaaatt gagctttcca gccgccgctt tatggggttg aaggagcgcc
     8461 gccgggcgct ggcgggacgt acgggctgtg gcgtatgcgg cgtggagcaa cttaatgaca
     8521 tcggaaaacc ggtgcagccg ctaccgttca cccagacgtt tgatctcaac aaactggatg
     8581 atgcattacg tcatctcaac gattttcagc cagtggggcg actgactggt tgtactcacg
     8641 ccgctgcctg gatgttgcca tctggcgaac tggtcggcgg gcatgaagac gtgggtcgcc
     8701 atgtggcgct ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc tggcagcaag
     8761 gtgcggtact ggtttccagt cgtgccagtt atgaaatggt gcaaaagtcg gcgatgtgcg
     8821 gcgtagagat tttgtttgcg gtgtctgccg cgaccacgct tgctgtagaa gtggctgag
//
LOCUS       DAJTYH010000146         8763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTYH010000146 DAJTYH010000000
VERSION     DAJTYH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..182)
                     /locus_tag="OO609_003595"
                     /pseudo
     CDS             complement(1..182)
                     /locus_tag="OO609_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            186..761
                     /locus_tag="OO609_003596"
                     /pseudo
     CDS             186..761
                     /locus_tag="OO609_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            812..1762
                     /locus_tag="OO609_003597"
                     /pseudo
     CDS             812..1762
                     /locus_tag="OO609_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2165..2746)
                     /locus_tag="OO609_003598"
     CDS             complement(2165..2746)
                     /locus_tag="OO609_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634870.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2793..6650)
                     /gene="sigA"
                     /locus_tag="OO609_003599"
     CDS             complement(2793..6650)
                     /gene="sigA"
                     /locus_tag="OO609_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6634871.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(7011..8015)
                     /locus_tag="OO609_003600"
                     /pseudo
     CDS             complement(7011..8015)
                     /locus_tag="OO609_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            8000..8377
                     /locus_tag="OO609_003601"
                     /pseudo
     CDS             8000..8377
                     /locus_tag="OO609_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8416..>8763)
                     /locus_tag="OO609_003602"
     CDS             complement(8416..>8763)
                     /locus_tag="OO609_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6634872.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         2176 a         2023 c         2089 g         2475 t
ORIGIN      
        1 ggcattttca gcgatgttgt tgtcgatttc cacccagcca tcgttcgcat agtacgtcag
       61 tgccggccac tggttaagtg cgtacgcgaa cgccttcgcc aactctgagt gtcgcgactg
      121 ggtcttcatc ttttccgcaa ccagctttcc agggatttca acagcggttt cgtttttcgc
      181 tgacaccatg gagggagtgg ttaaaagagc aacgagaaag tggtaattat aacgccagcc
      241 ggatatggcg ggaaatggtg gcccggggat ttacaggcag tgaaaccatc gtcagggatg
      301 cggttgccaa atggcgtaaa ggctggaacc caccggttac tactgccgtc agacttcctt
      361 cagtctcccg ggtaagccgc tggttgatgc cctggagaat aaccagggat gaagaaaatt
      421 atgcttcccg atttattagt ctgatgtgtg aaaaagaacc tgagctgaaa atagcgcagc
      481 aactggcact cgagttctac cgtattctga aaacccaaaa taaatcacag ctaagcagtt
      541 ggttcactcg agtccacgaa agcggttcag cagaatttcg gcgcgtggcg gcggggatgg
      601 aagctgatgc tgcagctata tgtgaggcaa tcagtagtcg ctggagtaat ggtgttgtcg
      661 aaggtcatgt aaatcgcctg aagaagatgc tgaaacgcca gatgtatggt cgagcaggat
      721 ttgaactact caggcagagg gtaatgagtc cactggcatg aaaatctcca cccaggttgc
      781 ggaagagcca cttttcatcc gtcgttgaca gttataccgc cagtcagaaa tataccgtcg
      841 tcagggcgtg gagctgagcc gtgccacact gggtcgctgg accggtgccg ttgctgaact
      901 gctggagccg ctgtatgacg tcctgcgcca gtatgtactg atgcccggta aagttcatgc
      961 ggatgatatc cccgttccgg tccaggagcc gggcagtggt aaaacaagaa ctgcccggct
     1021 gtgggtctat gtccgtgatg accgtaacgc cggttcacag atgcccccgg cggtctggtt
     1081 cgcgtacagc ccggaccgga aaggtatcca tccacagaat cacctagccg gttacagcgg
     1141 tgtgcttcag gcagatgctt acggtggtta ccgggcgtta tacgaatccg gcagaataac
     1201 ggaagctgcg tgtatggccc atgcccggag aaaaatccac gatgtgcatg caagagtgcc
     1261 aaccgacatc accacggaag ccctgcagcg tatcggtgaa ctgtatacca tcgaggcaga
     1321 agtccgggga tgttcagcag aacagcgtct ggcggcaaga aaagcaagag ccgcgccact
     1381 gatgcagtca ctgtatgacc ggatacagca acagatgaaa acactgtcgc gtcactcgga
     1441 tacggcaaaa gcgttcgcat acctactgaa acagtggaag gccctgaacg tgtactgcag
     1501 taatggctgg gtggaaatcg acaacaacat cgctgaaaat gccctgcggg cggtcagtct
     1561 gggtcgtaaa aacttcctgt tcttcggctc tgaccatggt ggtgagcggg gagcgctact
     1621 gtacagcctg atcgggacgt gcaaactgaa tgacgtggat ccagaaagct accttcgcca
     1681 tgtgcttggc gtcatagcag actggccggt caaccgggtc agcgaactgc ttccgtggcg
     1741 catagcactg ccagctgaat aacacatccc cgtcaatacg gccctcgctg tacgcttact
     1801 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
     1861 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
     1921 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
     1981 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
     2041 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
     2101 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
     2161 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
     2221 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
     2281 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
     2341 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
     2401 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
     2461 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
     2521 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
     2581 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
     2641 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
     2701 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
     2761 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     2821 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     2881 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     2941 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     3001 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     3061 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     3121 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     3181 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     3241 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     3301 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     3361 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     3421 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     3481 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     3541 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     3601 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     3661 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     3721 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     3781 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     3841 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     3901 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     3961 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     4021 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     4081 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     4141 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     4201 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     4261 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     4321 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     4381 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     4441 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     4501 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     4561 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     4621 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
     4681 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
     4741 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
     4801 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
     4861 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
     4921 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
     4981 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
     5041 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
     5101 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
     5161 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
     5221 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
     5281 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
     5341 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
     5401 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
     5461 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
     5521 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
     5581 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
     5641 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
     5701 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
     5761 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
     5821 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
     5881 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
     5941 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
     6001 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
     6061 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
     6121 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
     6181 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
     6241 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
     6301 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
     6361 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
     6421 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
     6481 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
     6541 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
     6601 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
     6661 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
     6721 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
     6781 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
     6841 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     6901 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     6961 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     7021 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     7081 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     7141 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     7201 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     7261 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     7321 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
     7381 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     7441 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     7501 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     7561 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     7621 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
     7681 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     7741 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     7801 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     7861 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     7921 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     7981 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     8041 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     8101 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     8161 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     8221 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     8281 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     8341 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     8401 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     8461 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
     8521 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     8581 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     8641 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
     8701 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     8761 cag
//
LOCUS       DAJTYH010000147         8758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTYH010000147 DAJTYH010000000
VERSION     DAJTYH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..246
                     /locus_tag="OO609_003603"
     CDS             <1..246
                     /locus_tag="OO609_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6634873.1"
                     /translation="GYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDD
                     ITGRVHKDRKRLTGDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(354..1712)
                     /gene="hprS"
                     /locus_tag="OO609_003604"
     CDS             complement(354..1712)
                     /gene="hprS"
                     /locus_tag="OO609_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6634874.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(1712..2383)
                     /gene="hprR"
                     /locus_tag="OO609_003605"
     CDS             complement(1712..2383)
                     /gene="hprR"
                     /locus_tag="OO609_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6634875.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2516..2929
                     /gene="hiuH"
                     /locus_tag="OO609_003606"
     CDS             2516..2929
                     /gene="hiuH"
                     /locus_tag="OO609_003606"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6634876.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3038..4042
                     /gene="msrP"
                     /locus_tag="OO609_003607"
                     /pseudo
     CDS             3038..4042
                     /gene="msrP"
                     /locus_tag="OO609_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4043..4678
                     /gene="msrQ"
                     /locus_tag="OO609_003608"
     CDS             4043..4678
                     /gene="msrQ"
                     /locus_tag="OO609_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6634877.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            4936..5586
                     /gene="zinT"
                     /locus_tag="OO609_003609"
     CDS             4936..5586
                     /gene="zinT"
                     /locus_tag="OO609_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6634878.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(6664..6933)
                     /locus_tag="OO609_003610"
     CDS             complement(6664..6933)
                     /locus_tag="OO609_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6634879.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            6990..7658
                     /locus_tag="OO609_003611"
     CDS             6990..7658
                     /locus_tag="OO609_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6634880.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            7974..>8758
                     /locus_tag="OO609_003612"
     CDS             7974..>8758
                     /locus_tag="OO609_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6634881.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTA"
BASE COUNT         2524 a         1971 c         1777 g         2486 t
ORIGIN      
        1 ggttattcta tttgcgcatt cccagacgcc gcagacaaac aaacgccaga tattggctat
       61 atgccgggtc atctcacctg gtacttcggc gaagagttga agaaaatggg catgaacatc
      121 attaatgacg acatcaccgg gcgagtacat aaggaccgta aacgtctcac cggcgacagt
      181 ccttttgcag cgaatgcgtt gggtaaactg gcggcgcagg aaatgctggc agcttacgcg
      241 agttaatccc tgttacattg tgatgtaagc gcataatcaa tcgcagccac gttaatactc
      301 acgacgtggc tgcgtttttt cacggttaac ttatggcgta ctgaagcccg atgttaattt
      361 ctttgcggta acgtaattcg gaacacgtta tgcttactga gatagtgata cgtagcactt
      421 cctccatgta attcggcaat cgctttgact aaagaaaggc caagtccctg acctacggaa
      481 tgacgcgaat tatctccccg ccaaaatcta cggaagagtt tttcaggctc attaattttc
      541 gttccaggac tggcgatatc aatattaaga gagccgttgg catcaagaaa actggttata
      601 tgaatacgcg atttttctgg tgaatatcga atggcgttaa caataagatt cgataacatt
      661 cgttgcagta aaattttatc cgcaaagatt tgttgattgc acttgacctt aaagcaaatc
      721 tctttctcgt ctgaaagata ttcaagatag tccaaaagat tttcaacttc cttattgaga
      781 gaaagcgaat ccagtttcac caaaacattg tttttgtctg ccctggcaag aaacagtata
      841 ttctctgtta accgcgaaat attttccagc tcttcaatgt ttccggcaat tgttttttga
      901 tattcagcga tacttctgat ttgactgagc gtaacctgat tctgacccag taatgcatta
      961 attggcgttc taagttcatg agcgagatcg tcagcaaatt gactaaggcg ctcaaaatct
     1021 ttgactaaag catgatgcat tttattcaac gcctgcccaa gaggttttag ttctctcggt
     1081 aatgcgttaa cctcaaccgg cactcggcta tcgttataat tcagcgcttc cgttacgcca
     1141 ctcaactttt tgatctctcg caaccccgtc ctgattaaca acggacttaa tactgagcac
     1201 agtataatgg cgacaatgca aattataatg ctattaattt tatactgttc aagcatatta
     1261 tgtctggctg aagccaattt agccacagta accgttaatg atggcgaaac ttcatcaatg
     1321 ttaattcgta aagcatctat ctctgtatca ttaataatgc ttctgtaaat gcctgctgcg
     1381 ctgattgtct cactagcagg tatgttattt aacatgtcat cactgacatt tgtccggtta
     1441 acaattttat tgatgccatc actatgaatg atcaaaatat cctgactaac atccatcatc
     1501 cggttaaagt acacaggtaa cgtatctgga tttaccccat caattaacaa ctgcttgatc
     1561 tgcgctgtcc ggttaatgag tgttgtatca tcgcgccatt tcaactcact tgccaggcca
     1621 ttatagagag tccatacaat tccagcgcca gcaacagact gcagaaatat aaaaagcaag
     1681 gttaaacgga cggttataga taatcttttc attatttttt taccgctacg aatgaatagc
     1741 ccatcccccg gattgtggcg attagctttt caggaaaagg atcatcaact tttgcgcgga
     1801 gcctgcgaat ggcgacatcc accgtattgg tatcactatc aaagttgatt ccccaaattt
     1861 cactcgcaat aaccgttctg ggtataatct cgccagctct ggaggccagt agccaaagta
     1921 actgaaactc cttgcgcgtc agtgtaatac tgatattgtc cctgctgaca ctttgactga
     1981 cagagtccat ttttaagcca ctgatttcta atgttgaatt cagagcgtga tgttgcctta
     2041 attgtgcccg aacccttgct agcaactcag aaaatgaaaa aggttttacc agataatcat
     2101 ttgccccact gtccaaccct ctgactctgt catcgacaga atcccttgca gtaaggcaaa
     2161 taacaggggt ttgctttgcc gttcttaacg tttgtaagat ctgccagcca tccatacccg
     2221 gaagcataat atccagaatg atcaatgcat aatcatcctt cagcgcaaga taaagcccat
     2281 ctctgccatc agaaacggca tcaatgacat aacccgcttc ggaaagcccc tgcgttaccc
     2341 attcctgggt cctttgatta tcttcaataa gtagaatctt catgaaaata atatgccata
     2401 aatcatatgt tatagaaaca acctggttca ttacaaaatt gtaatgctgc tgtaaggtta
     2461 ccctggacgt tttttcgcta tcctcaaaac tcattcacat gacaaggata taaacatgtt
     2521 aaagcgttat ttagtactct cggtagcaac ggcagccttt tcattacctt ctttggttta
     2581 tgccgcacaa caaaacattc ttagcgtgca cattttgaac cagcaaacag gaaaacccgc
     2641 tgccgacgtg acagtcactc ttgaaaagaa agcggataac ggctggttac aacttaatac
     2701 cgccaaaaca gataaggatg gacgaattaa ggcactgtgg cccgagcaaa ctgcgactac
     2761 tggcgattac cgtgtcgtat ttaaaaccgg ggactatttc aagaaacaaa atcttgaaag
     2821 tttcttccct gagattcccg ttgaatttca tattaataaa gtgaacgagc attatcatgt
     2881 gcctttgtta ctcagccaat atgggtattc aacctatcgt ggcagttaat gcgtaaatat
     2941 gtaaaagctg atctctgcta ctgttaggga ggtgactccc tgacagatta acagtaaaca
     3001 gctcttgttg gttaacgaca aaaaagtgtg atggcttatg aaaaagaagc aatttttaaa
     3061 agaatcagat gttacggccg agtcggtatt ctttatgaag cgtcggcagg tgttaaaagc
     3121 actgggcatc agcgcagctg cattttcttt gcctcacgct gcgcatgccg atctgcttag
     3181 ctggtttaaa gggaacgatc gtccgcccgc ccccgccgga aaaccgctgg agttcagcaa
     3241 gcctgccgcc tagcaaaata acctgccact gacgccagca gataaagtct ccggttataa
     3301 caacttctat gaattcgggc tggataaaga cgatcccgcg gctaatgctg gtagcctgaa
     3361 aaccgatcca tggacactga aaatcagcgg cgaagtggca aaaccattga ccctcgatca
     3421 tgatgattta acccgtcgct tcccgctgga agagcgtatt tatcgtatgc gctgcgtgga
     3481 agcgtggtcg atggtggtgc cgtggattgg ttttccgcta cacaaattgc tggcgcttgc
     3541 cgaacccacc agcaatgcga agtatgtcgc tttcgaaacg atttatgcgc cggaacagat
     3601 gcctggccag caggaccgct ttatcggcgg cgggctgaaa tatccttatg tcgaaggatt
     3661 gcgtctcgac gaagcaatgc atccgctcac actgatgacc gtaggtgttt atggcaaggc
     3721 gttaccaccg caaaatggcg cgccggtgcg actgattgtg ccgtggaaat atggctttaa
     3781 agggattaaa tcgatcgtca gtattaagct gacccgcgag cgtccgccaa ccacctggaa
     3841 tctggcagcg cctgacgaat acggttttta cgccaacgtt aatccgcatg ttgatcaccc
     3901 gcgctggtca caggctaccg aacgatttat tggttcaggc ggcattctcg atgttcagcg
     3961 ccagccaaca ctactgttta atggttacgc cgaccaggtg gcatcgctgt atcgtggcct
     4021 ggatttgcgg gagaatttct aaatgcgtct gacagcaaaa caagtgatat ggctgaaagt
     4081 ttgcctgcat cttgccggat tgttgccgtt tctctggctg gtctgggcga tcaatcacgg
     4141 tggactgggt gccgatccgg tgaaagatat tcagcatttt accggtcgca ctgcactgaa
     4201 atttttgctt gcggccttgt taatcacacc tctggcacgc tacgcaaaac agccgttatt
     4261 gatacgcact cgccgcctgt taggcttatg gtgctttgcc tgggcgacac tgcatttaac
     4321 cagttacgca ttgctggagt tgggcgtgaa taatctggcg ttattaggta aggagttaat
     4381 tacccggcct tatttaacgt taggaattat cagctgggtt attttgcttg ctttagcgtt
     4441 cacttcaacc cagtcgatgc agcgaaaact cggcaaacac tggcaacagt tgcataactt
     4501 cgtctatctt gtcgcgatcc tggccccaat acattatctg tggtcagtga agattatttc
     4561 accgcagcca ctcatctacg ccgggctggc tgtactgctt ttagccttac ggtataagaa
     4621 gttgctttcc cttttcaacc ggttacgcaa gcaggcccac aacaaattat ctctataact
     4681 tcatattgcc gacaaacttc gcgttatccc ggccggatgc ggcgcgagcg ccctatccgg
     4741 cccacacaga tcgcatcaag ttatgtttcg tttgtcatca acctttcatc accagaataa
     4801 tttattttat ttcaataaga tagttcaata cacctgagaa taccatgctc tcatttccta
     4861 atgagttgtt gcattttttc atatgttaca atataacatt acacataatg tacattaact
     4921 ctggaggaaa ttgttttggc gattcgtctt cataaactgg ctgttgcttt aggtgtcttt
     4981 attgttagcg ctcctgcctt ttcgcatggt catcattctc atggcaaacc cttaacagag
     5041 gtcgaacaaa aagctgctaa tggtgttttt gatgatacca atgtacaaaa ccgaacgctc
     5101 agtgactggg atggagtctg gcaatccgtt taccctttac tgcaaagtgg caaacttgat
     5161 cccgtctttc agaagaaagc ggatgcagat aaaaccaaaa catttgctga aattaaagat
     5221 tattatcgca aaggttatcc gacagatatc gaaatgattg gcattgagga cggcattgtc
     5281 gaattccata gaaataatga aacaacatcc tgtaaatatg attacgatgg atacaaaata
     5341 ctcacctata aatcaggcaa gaaaggcgtt cgttacttat tcgaatgtaa agatcctgaa
     5401 agcaaagccc ctaaatatat acaatttagc gatcatataa ttgcaccacg aaaatcatct
     5461 cattttcaca tttttatggg taatgattcc cagcaatctt tattgaatga aatggaaaac
     5521 tggccaacgt attatcccta tcagttgagt agcgaagaag tggtcgagga gatgatgtct
     5581 cattgatttt tttgcgctga cttcagttat ttaacagaac agtgcctggt agtaacaaca
     5641 ataatttacc aagacaaata agtaagacaa actcagacac gctcatactt gagtcgtgtc
     5701 ttttttatta tgtctgtaga aaaaaactga gaaaaaggct aacaataaat acacaactca
     5761 gccatacaga ctggcagcgc aaatgtttcc aggagcaagt catctgtcat ggtaaagcta
     5821 accagaaata taggttaatt ttggtgttta aacctttttt acttgttagt atgatacaaa
     5881 ggctttcaaa aaagctggga aattcaacaa ctcttggaaa ttatggactt tgtcccgcgg
     5941 ctttaatctg acggccgcgt tccttttttg gctcaattat tgaccccttc tctacgcaac
     6001 ttcagttccc gccagcaact ttgcggcagt ttcataggat caatgtctaa aagaataatg
     6061 gtgaccgata agaaaacgac tgaataactg tagattttcg ctcgaaacct tcctgtcagc
     6121 tccatagcga atcaagcgct gaatgtcaca gtatcgaaca gaaaacagtg acgatctaac
     6181 ccttcaagaa tattctacga ttgttctgtt taggaaaagc aaggcgggaa gtcgggagat
     6241 aagtcattga taaaatggcg gagagagggg gatttgaacc cccggtagag ttgcccctac
     6301 tccggttttc gagaccgatg atacagagtc gaaaaatcaa agagttaaaa atttttcttg
     6361 gaaaaaatgt cttaaaatcg tacacgaaaa atcaatgaat tacataacat taaagaagaa
     6421 tattccaagc cttcttgagt ccaccacctg ttttcaggtg tcattcagac gtgatttgcc
     6481 ccagattggg aaagcacgct tttttctcag cttattccgt tttgcctgtg tgttgaataa
     6541 ttccactgca gttatataaa atcggtaacg gctggaaatc attcaatact cgcactatcg
     6601 aaagtttacc agccaaccgc agcacgtcct gcatacgtcg tgtctgcggt ttttcttttt
     6661 cgcttacatt gtgtctggtt cttccggcca ctcaatatca ggtgcagttg atgtatcaac
     6721 acggttcagc aacacccgat acttcttcca ggcttccagc aacgagtttt cttcctccgt
     6781 tgcgatctcc agatctacag catcctgaag tggcgcaata tgctcactga atccctggat
     6841 gtagaactgt gtggtgacgg tcttccagcc attcggctcc tgctgtatcg aagcatacca
     6901 ggctatttca atatcgctat gctgcggcag catttaaccc cttgtaattc atcgccataa
     6961 ttgatttaat tcacaaataa aactataata tggtgaaatt aatgaaaaaa aacacagatg
     7021 atggggctaa aatttacaca ccacttaccc taaagcttta tgactggtgg gttttgggag
     7081 tatcaaatcg gcttgcatgg ggatgtccta caaaggaaca ccttcttcca cactttctgg
     7141 aacatttagg taacaaccat ctggatattg gtgttggaac tgggttttac cttactcacg
     7201 tacctgagag tagtctgata tctttaatgg atttgaacga agctagcctg aacgcggcat
     7261 ctacaagggc tggggaatca aaaattaaac ataaaattag ccatgatgtt tttgaacctt
     7321 atcctgcggc gttacatggt caatttgatt ccatttccat gttttacctt cttcactgcc
     7381 tgcctggaaa tatatctaca aaaagctgtg taatacgcaa tgcggcgcag gccttaactg
     7441 atgatggaac tctatacgga gccacaattc ttggcgatgg agttgtgcac aatagcttcg
     7501 gtcaaaaact gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca aaagattccg
     7561 aagaaggctt aacacatata ctctcagagc atttcgagaa tgttaaaacc aaggttcaag
     7621 gtactgtagt aatgttttcc gcttcaggga aaaaatagca tccaaccgca gcacgttctt
     7681 gcttaagacg tgctacggtt tcctttacga tttggggttg cgactttacc tctattgata
     7741 atgcattccg gcagaacgtt caaatatgaa cgtacgatat ttaactaacc gaaaaacaaa
     7801 atatagcaca ggcgagacat ttaccatcta agaaaatagt atcgtttttt tacagttaaa
     7861 tcaacatcac ttccttaaaa tgaaaacaat aaatttcaat cctgaaacat tttatttcag
     7921 cttattattc cccagtatca cattttatca ggatatcctt ctgcgaggtt attatgcttc
     7981 ctgtaaataa tcccccccta tccactggaa acgtctcttt ttacagaact acatcaatcg
     8041 acaatgttca caataattat ctctccgaat gggttgaatg gactaaaaac agcatttccg
     8101 gagaaaacag ggaaactgct tttacccggc tccaattatg tctggagaac agtgaaacat
     8161 cgttggactt atcttgttta ggtctcagat ctctaccacg attgcctgac aatcttgatg
     8221 aaattaatgt aagcaataac caactatcaa tgctccccga gctaccaagg gcattgaaag
     8281 agctgaatgc aagcagtaat caattatctg cacttcctga attaccagtg tcgctggaat
     8341 atataaatgt gagtgataac catttgttcg cacttcctga attacctgcg tcactagaat
     8401 atattaatgt aagtgacaat cacctgtctg tacttccgag gttaccaatg tcattggaat
     8461 tacttgatgc agccagaaat gctttggaag taataccaga ttttccagaa agagatgatc
     8521 atattataag aatattctgg cttaatcaga accggatcac ggcaattccg gaaagcatac
     8581 ttggcctcag ttctgatagc gttgtcaatc ttagagaaaa tcaactatct cccagaataa
     8641 tgcaaacttt gttacaacaa accgcccaac cggactacca cggcccacgg atttacttct
     8701 ccatgagtga cggacaacag aatacactcc atcgccccct ggctgatgcc gtgacagc
//
LOCUS       DAJTYH010000148         8713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTYH010000148 DAJTYH010000000
VERSION     DAJTYH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            362..1480
                     /locus_tag="OO609_003613"
     CDS             362..1480
                     /locus_tag="OO609_003613"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6634882.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1546..1794
                     /locus_tag="OO609_003614"
     CDS             1546..1794
                     /locus_tag="OO609_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6634883.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1859..2227
                     /locus_tag="OO609_003615"
     CDS             1859..2227
                     /locus_tag="OO609_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6634884.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2321..2974
                     /gene="nfsB"
                     /locus_tag="OO609_003616"
     CDS             2321..2974
                     /gene="nfsB"
                     /locus_tag="OO609_003616"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6634885.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3082..4329
                     /gene="ybdG"
                     /locus_tag="OO609_003617"
     CDS             3082..4329
                     /gene="ybdG"
                     /locus_tag="OO609_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6634886.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4397..5773)
                     /gene="pheP"
                     /locus_tag="OO609_003618"
     CDS             complement(4397..5773)
                     /gene="pheP"
                     /locus_tag="OO609_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6634887.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5874..>8713)
                     /gene="cusA"
                     /locus_tag="OO609_003619"
     CDS             complement(5874..>8713)
                     /gene="cusA"
                     /locus_tag="OO609_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6634888.1"
                     /translation="PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVD
                     RSGKHDLADLRSLQDWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLA
                     EVKSALDASNQEAGGSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDV
                     AKVQIGPKMRRGIAELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVE
                     IVTTYDRSQLIDRAIDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAF
                     IVMPFQGLNANIMSLGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRW
                     QVITDASVEVGPALFISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAI
                     VVIPILMGYWIRGKIPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLW
                     PLNKVGGEFLPQINEGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTG
                     KAETATDSAPLEMVETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRN
                     RIDMLSTGIKSPIGIKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINV
                     EINREKAARYGMTVADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALR
                     QLPILTPMKQQITLADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQK
                     AIAEKVQLKPGTSVAFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLI
                     ISSVPFALVGGIWLLWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSL
                     NNPQTFSEQKLDEALYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPM
                     IGGMITAPLLSLFIIPAAYKLMWLHRHRVRN"
BASE COUNT         2136 a         2414 c         2097 g         2066 t
ORIGIN      
        1 ggcttcatct ttccagacct cagagtaaac ctgaaatcgg aaagcttcag gcacccgcag
       61 ttatcttacc cttcctcaac aaaatagctg tttatttata cagcaaatcc tgtttcggac
      121 ttctcactcc ccatttgatc aactgaagct gaacccgttt ttttctgcat gcctcaatcg
      181 tcaaaattct tcgccaccag ccagctctgc cataacttgc cacattgaaa aagtagccaa
      241 agtgcttcag gcaccatcag ttcaccatca ccaaaaaaaa gaaacattcc agaacttccg
      301 catctcgccc agccgctccg acttctatac tgaatagaaa acgtcaactt aagagaaacc
      361 tatgccatta cccgattttc atgtttctga accttttacc ctcggtattg aactggaaat
      421 gcaggtggtt aatccgccgg gctatgactt aagccaggac tcttcaatgc tgattgacgc
      481 ggttaaaaat aagatcacgg ccggagaggt aaagcacgat atcaccgaaa gtatgctgga
      541 gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc gggcagtttt cagcgatgca
      601 gaaagtcgta ttgcaggcag ccgcagacca tcatctggaa atttgcggcg gtggcacgcc
      661 cccgtttcag aaatggcagc gtcaggaggt atgcgacaac gaacgctatc aacgaacgct
      721 ggaaaacttt ggctatctca tccagcaggc gaccgttttt ggtcagcatg tccatgttgg
      781 ctgtgccagt ggcgatgacg ccatttattt gctgcacggc ttgtcacggt ttgtgccgca
      841 ctttattgcc ctttccgccg cgtcgccata tatgcaggga acggatacgc gttttgcccc
      901 ctcacgaccg aatatttttt ccgcctttcc tgataatggc ccgatgccgt gggtcagtaa
      961 ctggcaacaa tttgaagccc tgtttcgctg tctgagttac accacgatga tcgacagcat
     1021 taaagatctg cactgggata ttcgccccag tcctcatttt ggcacggtgg aggttcgggt
     1081 gatggatacc ccgttaaccc ttagccacgc ggtaaatatg gcgggattaa ttcaggccac
     1141 cgcccactgg ttactgacgg aacgcccgtt caaacatcag gagaaagatt acctgctgta
     1201 taaattcaac cgtttccagg cctgtcgcta tggtctggaa ggcgtcatta ccgatccgca
     1261 cactggcgat cgtcgaccgc taacggaaga taccttgcga ttgctggaaa aaatcgcccc
     1321 ttcagcacat aaaattggcg catcgagcgc aattgaagca ctgcatcgcc aggtcgtcag
     1381 cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc gatggcggct cgctgattgg
     1441 gctggtgaaa aagcattgtg agatctgggc cggtgactaa gccgggaatt gccttacaga
     1501 agaatatccc tgacaatggt gttttaactt acatttaaca atgttatgaa acacccttta
     1561 gaaaccttga ccaccgcagc aggcattttg ctgatggctt tcctctcttg cctgctgctg
     1621 cccgcccccg cactggggct gacgctggca caaaaactgg tgaccatgtt ccatctgatg
     1681 gatcttagcc agctttacac tttattgttt tgtctgtggt ttttagtgct gggcgctatt
     1741 gagtattttg ttctgcgctt tatctggcga cgctggttct cgctggcgga ttaaacgtcg
     1801 attatcgccg aacttcgcgg catactttgc ttattctcct tcgcctaaag gaacgtttat
     1861 ggataagcaa tcactgcacg aaacggcgaa acgcctagct cttgagttac cctttgtcga
     1921 gctttgctgg ccttttggcc cggagttcga tgtttttaaa attggcggca agatttttat
     1981 gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg aagtccgatc cacaaaaatc
     2041 cctgttaaat cagcaaatat acccaagcat taagccaggg tatcacatga ataaaaagca
     2101 ctggatttcg gtgtattccg gcgaggaaat ctccgaagcg ttacttcgcg atctgatcaa
     2161 cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc gatcaaaaaa gagtgcgtcc
     2221 aggctaaagc gggaatctat agcgcatttt tctcgcttac catttctcgt tgaaccttgt
     2281 aatctgctgg cacgcaaaat tacttacaca tggagtcttt atggatatca tttctgtcgc
     2341 cttaaagcgt cattccacta aggcatttga tgccagcaaa aaacttaccc cggaacaggc
     2401 cgagcagatc aaaacgctac tgcaatacag cccatccagc accaactccc agccgtggca
     2461 ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt gccaaatccg ctgccggtaa
     2521 ttatgtgttc aacaaacgta aaatacttga tgcctcgcac gtcgtggtgt tctgtgcaaa
     2581 aaccgcgatg gacgatgcct ggctgaagct ggttgttgac caggaagatg ccgatggccg
     2641 ctttgccacg ccggaagcga aagccgcgaa cgataaaggt cgcaagttct tcgcctatat
     2701 gcaccgtaaa gatctgcatg atgatgcaga gtggatggca aaacaggttt atctcaacgt
     2761 cggtaacttc ctgctcggcg tggcggcgct gggtctggac gcggtgccca tcgaaggttt
     2821 tgacgccgca atcctcgatg cagaatttgg tctgaaagag aaaggctaca ccagtctggt
     2881 ggttgttccg gtaggtcatc acagcgttga agattttaac gctacgctgc cgaaatctcg
     2941 tctgccgcaa aacaccacct taaccgaagt gtaattctct cttgccgggc atctgcccgg
     3001 ctatttcccc tcagattctc ctgatttgca taaccctgtt tcagccgtca tcataggctg
     3061 ctgttgtata aaggagacgt tatgcaggat ttaatatccc aggttgaaga tttagcgggt
     3121 attgagatcg atcacaccac ctcgatggtg atgattttcg gtattatttt tctgaccgcc
     3181 gtcgtggtgc atattatttt gcattgggtg gtactgcgga ccttcgaaaa acgtgccatc
     3241 gccagttcac ggctttggtt gcaaatcatt acccagaata aactcttcca ccgtttagct
     3301 tttaccctgc aggggattat cgtcaatatt caggcggtat tctggctgca aaaaggcacc
     3361 gaagcggcag atattctgac tacctgcgcg cagttgtgga tcatgatgta tgcgctgctt
     3421 tcagtcttct cgttgctgga tgttattttg aatctggcgc agaaattccc ggcggcatct
     3481 cagttaccgc tgaaagggat atttcagggg attaaactga tcggcgcaat cctggtcggc
     3541 attttgatga tctcgctgct gattggtcag tcgccagcga ttctaatcag cggtcttggt
     3601 gcaatggctg ccgtgctgat gttggtattt aaagatccga ttcttggtct ggtggcaggt
     3661 attcagcttt ccgcgaacga tatgctgaaa ctgggcgact ggctggaaat gccgaaatac
     3721 ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg tcaaagtgcg taactgggac
     3781 aataccatta ccactattcc cacctggtct ctggtttctg actcctttaa aaactggagc
     3841 gggatgtcag catctggcgg gcgacgtatt aagcgcagta tcagtattga tgtcaccagt
     3901 attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata aagcgcattt gttaaagcct
     3961 tatttaacca gccgccatca ggaaattaat gagtggaatc gccagcaagg ttctacggag
     4021 tcggtattaa acctgcgccg aatgaccaat attggaacct ttcgtgccta tctgaacgaa
     4081 tatctgcgta accatccgcg gattcgtaaa gatatgacct taatggtacg ccaactggct
     4141 ccaggtgata acggtttacc gctcgagatc tacgcgttta ctaacaccgt ggtgtggctg
     4201 gaatatgaaa gcattcaggc tgatatattc gatcacatat ttgcgattgt cgaagagttt
     4261 ggtctgcgac ttcatcagtc gccaaccggc aatgatattc gctctctggc gggtgcattt
     4321 aagcagtaat taaaaaaacc gctctcatcg aatggatgag agcggacatc gttttgtcgg
     4381 atgtagcgtg aatgccttat ttccgacgca gcgttttaaa tgccacaaac aggaatacaa
     4441 tccacaccgg cagcaggatc gctgacaagc gcatatcatc catcgtgcac atcagcagca
     4501 aaatcatgcc gaggaaggcg atgcaaagat agttgccgaa cggatagagc agcgccttaa
     4561 actgtgtttc acgcccctga cgtcgcatcg ccgcgcgaaa acgcagatgc gccaggcaga
     4621 tcataatcca gttcaacagc aacgttgcta ccaccagcgc catcagcaga ccaaacgctt
     4681 tttgcggcag cagatagttg attaacacca ccagcgaagt gatcgctccg gaaagcatca
     4741 gcgagttaat cggcacaccg cgacggctga cgcgagtcaa aaacttcggc gcattaccct
     4801 gcacagaaag gccaaacagc atgcggctgt tagagtaaac cccgctgtta tacaccgaca
     4861 gcgatgccac tagaatgacg aagttcagcg cagaagctac cacattgctg tcgagattat
     4921 ggaaaatcat cacaaacggg ctactgttgg atttcacttc cacccacgga tagagtgcca
     4981 gtaaaaccac cagtgaaccg atataaaaca gcaggatgcg atacaccacc tgatttaccg
     5041 cttttggaat gcttttttcc ggatcgcgcg cttcagcggc tgtaatccca atcagctcca
     5101 gaccgccgaa ggagaacata attaccgcca gcgacaaaat cagcccattc cagccggtgg
     5161 cgaagaaacc accgtagcgc cagaggttgt cgatactggc tttctcgccg ccgtaaccag
     5221 aaaacagcag ccacaggcca aagccgatca taccgatgat cgccagcact ttaatcagcg
     5281 caaaccagaa ctcggtttcg ccatataagc gcacgttcac cagattaacg gcgttgatga
     5341 taataaagaa ggcggcagcc caaatccacg ttggaacatc cgggaaccag tactgcatat
     5401 agatgcccgc agcggtcagc tctgccattc ccaccagcac gaacattacc cagtagttcc
     5461 agccagagag gaagcccgca aacggtcccc agtatttata ggcaaagtgg gcaaatgaac
     5521 cggataccgg ctcttcgacc accatctcgc caagctggcg cataatcagg aaagcgatga
     5581 tcccggcgac gccgtagccc agcaatacag ccggacccgc catctgaatc gccgggccaa
     5641 tgccaagaaa cagaccagta ccaattgcgc cacccagcgc aatcagttga atatgacggt
     5701 tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc agtatcttcc gatacggttg
     5761 acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt gaggggcacc ttttaacatt
     5821 tagtgcccat cgtcgcaaga cacaatccac acggttaaac gaggtatcct gctttaattc
     5881 cgtacccgat gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgac
     5941 agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg caatccggct catcacctct
     6001 gaaccagccc ccgttcccca cagaatcggc agcagaccgg cgataatcac cgccaccgtc
     6061 atcgctttcg ggcgcacgcg caggaccgcg ccgtgataca acgcctcatc cagcttctgc
     6121 tcgctgaatg tttgtggatt attcaacgac ggctcggcct ctatggcgtg acgtaaatac
     6181 atcagcatca ccacgccaaa ttcggcggcg accccggcaa gggcaataaa gccggtaccc
     6241 gtcgccacag aaagatgaaa gcccatccac cacagtaacc agatgccgcc caccagcgca
     6301 aacggtacgc tgctgataat cagcaacgct tcgcccaccc gacggaatgc cagatacaac
     6361 agcacgaaga tgatcatcag tgtcatcggc accatgagtt tcagcttatg gttggcgcgc
     6421 tccagtagct cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt
     6481 tcagctatcg ctttttgcaa atcgtgaacc accgacacca tgtcacgatc gcgggcatcg
     6541 atataaatcc agctcgtcgg gcgcgcattc tcggttttca gcatcgacgg tccggtagag
     6601 actttaacgt cggccacgtc tgccagggtg atttgctgct tcatcggcgt caggatcggc
     6661 aactggcgca gtgcctgcgg actatcgcgc cagctttgcg gataacgcag attaattgga
     6721 taacgggcaa tcccttccac cgtttcgcca accatcgccc cgcccaccgc agaagtcaca
     6781 aacaactgca catccgccac cgtcataccg taacgtgcgg ctttttcacg gttaatctca
     6841 acgttgatat agcgcccacc ttccagccgc tcagcaagcg cagaagctac gcctggcacc
     6901 gttcgcgcca cttcttcaat ttgctcagcc atcgcgtcga tatccgccag cacagtgccg
     6961 gaaactttaa tgccgatggg gcttttaatg ccggttgaga gcatatcgat acggttacga
     7021 attggcggca cccacagatt cgccagcccc ggcagacgca cggtgttatc cagttcctca
     7081 atgattttgt ccattgtcat gcctggccgc cactgatcct gtggcttaag ctggatggtc
     7141 gtttctacca tctccagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca
     7201 aatacccgcg ccacttcagg tacgctcata attagcttgt cggttttttg cagcatactc
     7261 gccgcctctg ctgcggaaat ccccggcagc gtcgatggca tatacaacaa gtcgccttca
     7321 ttgatctgcg gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc
     7381 gaaagcgccg ccaccagcag cgtggttttc ggccagtgca gtactttcag caacagcgga
     7441 tgataaacac gaatcaaaaa gcgattgagc ggattactgc tttccggcgg aattttgcca
     7501 cggatccagt aacccatcag gatagggatc actacgatcg ccagcagcgc cgcacccgcc
     7561 atcgcatacg ttttggtgaa cgccaacggg ccaaacagac gcccttcctg cccttccagg
     7621 gtgaagatcg ggataaacga caacgtgata atcagcagac tgataaacag cgccggcccc
     7681 acttcaacag acgcatcggt gatcacctgc cagcgcgttt tattatccag cgtggcgtca
     7741 ggatgctggt gctgccactc ttccagctgt ttatgcgcat tctcgatcat gacgatagcg
     7801 gcatcgacca tcgccccgac ggcaatcgca atgccaccca gcgacataat attggcattc
     7861 agcccctgga agggcatgac aataaaagca atacacaacc ccagcggcaa cgaaataatc
     7921 gccaccagcg ccgagcgcac atgccagaga aacggcgcgc agactaccgc caccacaata
     7981 aactcttcca gcaacttgcc gctgaggttg tcgatggccc ggtcaatgag ctggctgcgg
     8041 tcgtatgttg taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg
     8101 tccttcacgg cggcgatcac ttctcgggcg tttttgccgg atcgcaggat caccaccccg
     8161 cccgccactt cgccttcgcc gtttagttcg gcaatgcccc ggcgcatctt cgggccaatc
     8221 tggaccttcg caacatcgcg cagataaacg ggcacgccat tttcactggc ttttaaaacg
     8281 atgtgattaa agtcgtcgag cgtttgtaga tagccgctgg cgcgcaccat atattctgct
     8341 tccgccagtt cgattgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt
     8401 acttcggcga gactgatgcc atactgcgcc aggcgctgag gatcgataac cacctgatac
     8461 tctttcacca caccgcctac cgacgccact tccgcaacgt caggaatggt tttcagctca
     8521 tatttgagaa accagtcctg caatgagcgt aaatcggcaa gatcgtgctt accgctgcga
     8581 tccaccagtg catattcata gatccagcca acacccgtgg catctggtcc cagctcggcg
     8641 ctgactcccg caggcagctt accctgtacc tggttgaggt actccagcac ccgcgaacgc
     8701 gcccagtacg gat
//
LOCUS       DAJTYH010000149         8674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTYH010000149 DAJTYH010000000
VERSION     DAJTYH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            163..1794
                     /gene="oppA"
                     /locus_tag="OO609_003620"
     CDS             163..1794
                     /gene="oppA"
                     /locus_tag="OO609_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6634889.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1880..2800
                     /gene="oppB"
                     /locus_tag="OO609_003621"
     CDS             1880..2800
                     /gene="oppB"
                     /locus_tag="OO609_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6634890.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2815..3723
                     /gene="oppC"
                     /locus_tag="OO609_003622"
     CDS             2815..3723
                     /gene="oppC"
                     /locus_tag="OO609_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6634891.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3735..4748
                     /gene="oppD"
                     /locus_tag="OO609_003623"
     CDS             3735..4748
                     /gene="oppD"
                     /locus_tag="OO609_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6634892.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4745..5749
                     /gene="oppF"
                     /locus_tag="OO609_003624"
     CDS             4745..5749
                     /gene="oppF"
                     /locus_tag="OO609_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6634893.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5802..6131)
                     /locus_tag="OO609_003625"
     CDS             complement(5802..6131)
                     /locus_tag="OO609_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6634894.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6166..7626)
                     /gene="cls"
                     /locus_tag="OO609_003626"
     CDS             complement(6166..7626)
                     /gene="cls"
                     /locus_tag="OO609_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6634895.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7769..7942
                     /locus_tag="OO609_003627"
     CDS             7769..7942
                     /locus_tag="OO609_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6634896.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(7997..>8674)
                     /locus_tag="OO609_003628"
     CDS             complement(7997..>8674)
                     /locus_tag="OO609_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HCR6634897.1"
                     /translation="PVSESARLFTISVIISGITVFATSMTSIFGPLIRGGFNKLLKGN
                     NHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLPEDDIKQLEQRLGDNADVI
                     PGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKDMSSDVKTVLAVSDSKNLN
                     KIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLLNSGHGIFSDNDEQETKAD
                     SKESAQK"
BASE COUNT         2190 a         2079 c         2193 g         2212 t
ORIGIN      
        1 aggtgatcgt ctgaatcacc agaataaata aagtcggtga tagtaatacg taacgataaa
       61 gtaacctgac agcagaaagt ctccgagctt gtgcagggtc ccaatccggg attacacatg
      121 ctggttaata ccagtaatta taatgaggga gtccaaaaaa caatgaccaa catcaccaag
      181 agaagtttag tagcagctgg cgttctggct gcgctaatgg cagggaatgt cgcgctggca
      241 gctgatgtac ccgcaggcgt cacactggcg gaaaaacaaa cactggtacg taacaatggt
      301 tcagaagttc agtcattaga tccgcacaaa attgaaggtg ttccggagtc taatatcagc
      361 cgagacctgt ttgaaggctt actggtcagc gatcttgacg gtcatccagc acctggcgtc
      421 gctgaatcct gggataataa agacgcgaaa gtctggacct tccatttgcg taaagatgcg
      481 aaatggtctg atggcacgcc agtcacagca caagactttg tgtatagctg gcaacgttct
      541 gttgatccga acactgcttc tccgtatgcc agttatctgc aatatgggca tatcgccggt
      601 attgatgaaa ttcttgaagg gaaaaaaccg attaccgatc tcggcgtgaa agctattgat
      661 gatcacacat tagaagtcac cttaagtgaa cccgttccgt acttctataa attacttgtt
      721 cacccatcaa cttcaccggt gccaaaagcc gctatcgaga aattcggcga aaaatggacc
      781 cagcctggta atatcgtcac caacggtgcc tataccttaa aagattgggt cgtaaacgaa
      841 cgaatcgttc ttgaacgcag cccgacctac tggaacaacg cgaaaaccgt tattaaccag
      901 gtaacctatt tgcctattgc ttctgaagtt accgatgtca accgctaccg tagtggtgaa
      961 atcgacatga cgtataacaa catgccgatc gaattgttcc agaagctgaa aaaagagatc
     1021 ccggacgaag ttcacgttga tccatacctg tgcacttact attacgaaat taacaaccag
     1081 aaaccgccat tcaacgatgt gcgtgtgcgt accgcactga aactaggtat ggaccgcgat
     1141 atcattgtta ataaagtgaa agcgcagggc gacatgcccg cctatggtta tactccaccg
     1201 tatactgatg gcgcaaaatt gactcagcca gaatggtttg gctggagcca ggaaaaacgt
     1261 aacgaagaag cgaaaaaact gctggctgaa gcgggttata ccgcagacaa accgttgacc
     1321 atcaacctgc ggtataacac ctccgatctg cataaaaagc tggcgattgc tgcctcttca
     1381 ttgtggaaga aaaacattgg tgtaaacgtc aaactggtta accaggagtg gaaaacgttc
     1441 ctcgacaccc gtcaccaggg cacttttgat gtggcccgtg caggctggtg tgcagactac
     1501 aacgaaccaa cttccttcct gaacaccatg ctgtcgaaca gctcgatgaa taccgcgcat
     1561 tataagagcc cggcctttga cagcattatg gcggaaacgc tgaaagtgac tgacgaggcg
     1621 cagcgcacag ctctgtacac taaagcagaa caacagctgg ataaggattc ggccattgtt
     1681 cctgtttatt actacgtgaa tgcgcgtctg gtgaaaccgt gggttggtgg ctataccggc
     1741 aaagatccgc tggataatac ctatacccgg aatatgtaca ttgtgaagca ctaatggcaa
     1801 tacgtggggc aggagtgtcc tgctccacgg tgtctgattt ttatcgcatt acagaaggca
     1861 caggccagaa ggtagggcaa tgttaaaatt tattctacgt cgttgtctgg aagcgattcc
     1921 gacgctattt attcttatta ctatttcgtt ctttatgatg cgcctcgcac cgggaagccc
     1981 ttttaccggc gaacgtactt taccgccaga agtgatggcc aatatcgaag cgaaatatca
     2041 tcttaatgat ccgatcatga cacagtattt cagctacctg aaacaactgg cgcatggcga
     2101 tttcggtcca tcgtttaaat ataaagatta ttcggtcaat gacctggtgg catccagttt
     2161 tcccgtttct gccaaactgg gagccgccgc atttttcctt gcggtaatac tgggtgttag
     2221 tgctggcgtt attgccgcat taaaacaaaa caccaaatgg gactataccg tgatggggct
     2281 ggcaatgacc ggggttgtta tccccagttt tgtggttgcg ccattattag tcatgatatt
     2341 tgcgatcatt ttgcattggc tgccgggcgg tggctggaat ggtggggcgc ttaaattcat
     2401 gatattgccg atggtggcgt tgtcactcgc ctatatcgcc agtattgcgc gtattacccg
     2461 tggctctatg attgaagtat tacactccaa ctttattcgt actgcccggg cgaaagggtt
     2521 acctatgcgg cggatcattt tacgccatgc attaaaacct gctctgttac ccgtgctctc
     2581 ctatatgggc cccgcatttg tcggcattat taccggttct atggtcatcg aaaccattta
     2641 tggtttgccg gggattgggc aactgttcgt taatggcgca ttgaaccgtg actattcctt
     2701 agtgttaagc ctgaccatcc tggttggtgc gttaaccatt ttgtttaatg ccattgtcga
     2761 tgtgctatat gcggttatcg acccgaaaat ccgttactga tactggagct cgcgatgatg
     2821 ttaagtaaga aaaacagcga gacgctggaa aatttcagtg aaaagctgga ggtcgaaggg
     2881 cgcagcttgt ggcaggacgc acgtcgacgt tttatgcata accgtgcggc ggttgccagt
     2941 ctgatagtgc tggtgctgat cgcgttattt gtaatcctgg caccgatgct ttcccagttt
     3001 gcctatgacg atactgactg ggcgatgatg tccagcgccc cggatatgga gtccggtcac
     3061 tactttggta ctgactcatc cggtcgcgac ctgcttgtgc gcgttgcgat tggcgggcgt
     3121 atctcactca tggtcggtgt tgctgcggca ctggtggcag tggtcgtggg gacactttat
     3181 ggttcgcttt ccggttatct gggcggtaaa gtggattcgg taatgatgcg tctgctggaa
     3241 atcctcaact ccttcccatt catgttcttc gtcattttgc tggtgacctt tttcggccaa
     3301 aacatcctgc tgattttcgt ggcgattggc atggtttcct ggctggatat ggctcgtatt
     3361 gtgcgtgggc aaaccctgag tctgaagcgc aaagagttta ttgaggcggc acaagttggc
     3421 ggtgtatcga cgccgggcat tgttattcgc cacattgtgc cgaacgtact cggtgtggtg
     3481 gtggtctacg catcgctact ggtgcccagc atgatcctct ttgaatcttt ccttagcttc
     3541 ctggggttgg gtacgcaaga gccgttaagc agctgggggg cattgctgag tgatggcgcg
     3601 aactcgatgg aagtctctcc atggttactg ttgttcccag cgggattcct tgtggtgaca
     3661 ctgttttgtt tcaactttat cggcgatggc ttgcgtgatg ccctcgaccc gaaagatcgt
     3721 taaggagtgc agccatgagc gtaattgaaa ctgcaactgt gccgctcgca caacaacagg
     3781 ctgacgcact gctgaacgtg aaagatttgc gtgtcacctt tagtaccccg gacggcgacg
     3841 tcacggcggt caatgattta aatttttccc tacgtgccgg agaaacgctg ggcattgtag
     3901 gtgagtctgg ttcgggtaaa tcgcaaactg catttgcgtt gatgggcctg ttggctgcca
     3961 acggacgcat tggcggatcg gcaaccttca atgggcgtga aatcctcaat ttgccagagc
     4021 gtgaactcaa taaactgcgc gctgaacaaa tctcaatgat ttttcaggac ccaatgactt
     4081 cgttgaatcc ctatatgcgc gtcggtgagc agttgatgga agtgctgatg ctgcataaga
     4141 acatgagcaa agctgaggcg tttgaagagt cggtgcggat gctcgatgcg gtaaaaatgc
     4201 cggaagcacg taaacgcatg aaaatgtacc cgcacgaatt ttctggcggc atgcgtcagc
     4261 gagtcatgat tgcgatggca ttgctatgtc gacctaagtt gctgattgcg gatgaaccca
     4321 ctacagcgct ggacgtcacc gtacaggcgc agatcatgac gctattgaat gaactgaagc
     4381 gggaatttaa tactgccatc attatgatca cccacgatct tggcgtggtg gcggggatct
     4441 gtgacaaagt gctggtaatg tacgccgggc gcacgatgga atatggcaat gcgcgcgatg
     4501 tcttttatca acccgttcat ccttattcta tcggtttgct caacgcggtg ccgcgtcttg
     4561 atgcggaagg tgaaacaatg ttgaccatcc cgggtaatcc gccaaacttg ctgcgattac
     4621 cgaaaggttg cccgttccag ccacgttgtc cacatgcgat ggaaatttgt agtagcgcac
     4681 cgccgctgga agagtttacg cctggccgtc tgcgtgcttg ctttaaaccg gtggaggaac
     4741 tgttatgaat gctgtaactg aaggaagaaa agtcctcctt gaaatcgccg atcttaaagt
     4801 gcactttgaa atcaaagatg gcaaacagtg gttctggcaa ccgccgaaaa cgctcaaagc
     4861 cgtcgatggt gtcactcttc gcctgtatga aggggaaaca ttaggtgtgg taggggaatc
     4921 gggatgcggt aagtccacct ttgctcgcgc catcatcggt ttggtcaagg cgaccgacgg
     4981 tcatgttgcc tggttaggta aagagttgct gggcatgaag cccgatgaat ggcgtgccgt
     5041 tcgcagtgat attcagatga ttttccagga tccgttggca tcgctaaacc cgcgtatgac
     5101 catcggcgag atcatcgctg aaccactgcg tacttatcat ccgaaaatgt cacgccagga
     5161 agttcgcgag cgcgtgaagg cgatgatgct gaaagtcggg ttattgccta acctgattaa
     5221 ccgctatccg catgagttct ccggtgggca gtgccagcgt atcgggattg ctcgtgctct
     5281 tattcttgaa ccgaagctga ttatctgcga tgagccggtt tcggcgctgg acgtgtcaat
     5341 tcaggcgcag gtggttaacc tgctccagca gctgcaacgt gagatgggat tgtcattaat
     5401 ttttattgct catgacctgg ccgtggtaaa acacatttcc gatcgtgtgt tggtgatgta
     5461 tctcggccat gcagtagaac tggggaccta tgatgaggtc taccacaatc cactacatcc
     5521 ttacaccagg gcattgatgt cggcagtccc catacctgat ccggatctgg agaagaacaa
     5581 aaccatccag ttactggaag gggaattacc gtcgccgatc aacccgcctt ccggttgtgt
     5641 tttccgtacc cgttgcccga ttgccggtcc ggagtgcgcc aaaacacgtc ctgttctgga
     5701 ggggagtttc agacacgccg tttcttgcct gaaagtcgat ccgctttaaa aacaataagg
     5761 gctgacagtt gtcagccctt tttcacgcta aaagcgatta tttattcccg ccagatgata
     5821 tggcaaagtt tgtgatcttt ttcgcggcat aacagaatgc gggcgaaaac atcgctgatt
     5881 tcaccatctt cactgtccgc cagaccaatc accacttcgg caaaaaagtc cgggttcaaa
     5941 tcgaaatcaa catgttcctg ccagtcttcc gccggatcga ataactccgc gccgccgcgc
     6001 tcttcaaact gaagattgaa cagcagaaca tcagctggat cgagattgtc ggcagccagt
     6061 tcgagaaaaa tatcgtaggc ctgctcaagc gtttcatctt cagtcaggcg attgtttaga
     6121 tccatatcca tagtcactac ctgtttaacc tctgttggcg acgttttaca gcaacgggct
     6181 gaagaagtaa aacagtcgct cggcgacacg ttgccataat ggacgtttta gccataaacg
     6241 ggcatcgagc agacgtgaac gcgaaatata atcgtcctga acggcggcaa ggtctgcacc
     6301 aaaaccttta tcgtcgattg ccaaggtaat ctcgaaattc agccacagac tacgcatatc
     6361 aaggttaacg gtgccaacca gacttagttc gccatcgacc agcacgctct tggtatgcag
     6421 taacccgcct tcaaactgat aaattttaac cccagcagca agcagttccg taaagaatgc
     6481 gcgactggcc cagccgacca gcatcgagtc attttttcgc ggaaggataa tactgacatc
     6541 caccccgcgc tgcgccgccg tgcaaatcgc atgaagtaaa tcatcgcttg gcacaaagta
     6601 gggcgtggtc atgatcaaat attcatgcgc cgaataagcc gcagtcaata atgcctggtg
     6661 aatgagatct tccggaaagc cggggccaga agcaattgtg tgaatggtgt gaccgctggc
     6721 ctgttcaaac ggcataatat tgacatctgg tggtggcggc agaatacgtt ttccggtttc
     6781 aatctcccag tcgcaggaat aaataatccc catcgcggtg gcgatggggc cttccatacg
     6841 cgccatcaga tcaatccatt gccctacgcc cgcatcttgt ttgaagtagc gaggatcgac
     6901 catattcatg ctgccggtgt acgcgatgta attatcgatc atgatcatct tgcgatgttg
     6961 gcgcaggtcc atacggcgta aaaacacacg catcagattg acctttaagg cttcgaccac
     7021 ttcaataccg gcattacgca ttagctcggg ccacgggctg cggaaaaaag ccacactccc
     7081 ggcggagtcg agcatcaatc ggcaatgaat accacgccgt gcagccgcca ttaatgattc
     7141 agccacctgg tccgccatgc cgccgggctg ccagatataa aacaccatct caatattatg
     7201 gcgcgcgagc tggatgtcgc ggattaacgc ctgcatcaca tcatctgact cggtcatcag
     7261 ctgtagctga ttccctttga ccccagcgat cccctgacga cgctcgcaaa gcttgaataa
     7321 tggcgcagcg acactgctat tttcttcggc gaagatatgc ttacaggctt taaggtcgtt
     7381 aagccatttt gcggtggaag gccacatcgc tctggcgcgc tcagcgcggc gtttgcctaa
     7441 atggagctcg ccaacggcaa gataggcaat aattccgact aacggcagaa tgtaaataat
     7501 caacagccag gccatcgcgg agggaactgc gcgtcgtttc attagaatgc gtaaagttac
     7561 gcctgcaatg agcaaccagt atcccagaat ggccaaccaa ctcaccaacg tataaacggt
     7621 tgtcatagat tagaaatcct tttgaaagcg cattgttatg agtttacgca tatggattca
     7681 tctggcaaat aaaaacgcgg gaaaagcact ggtcagcgtc gggggcgggg gtataatgac
     7741 cattctgtta ttgcatagag tagttaacat gaagcggagt agaacggaag tggggcgctg
     7801 gcgcatgcag cgtcaggcta gccgacgtaa atcgcgttgg cttgaggggc aatcgcgccg
     7861 aaatatgcgt atccacagca tcaggaagtg cattctaaac aaacagcgta actcgttatt
     7921 gtttgcgatc tacaatatct aaatgtactg ggcaccattg cggtgcccaa taagtttact
     7981 cttccgtctt taaactctat ttttgcgccg attctttact gtcagctttc gtttcttgtt
     8041 catcgttatc gctgaaaata ccatgacctg agttcaacaa cattgaaacg agcatatcat
     8101 tattaatctc ttcaccattt aaaactcgcg ccagaatttc gctgccaaac agttgcggtg
     8161 agagaatgat atccggatgt accatcttaa tcttattcag gtttttgcta tcactgacgg
     8221 cgagaactgt tttgacatca ctgctcatat cttttgccga gagtacaaca aacgcgttat
     8281 ctgcatcgtt atcactcagc gccagaatgg cccggcaacg atcgattccc gctttcttta
     8341 atactgaact gtcattactg tcaccgggga taacatcagc gttatcgcct aagcgttgct
     8401 caagttgctt gatatcatct tcaggcaagt tgctgataac cgttacgttt tgtccgcgtt
     8461 gattcagttg caggatcgta ttgatggcga gaatcgaatg tccgcaaaca ataaaatgat
     8521 ctttacgatg cattgtatga ttgtttcctt ttaaaagttt gttgaatccc ccgcggataa
     8581 gcggaccaaa aattgaggtc atagatgtgg caaaaacggt aatgccggaa ataatgaccg
     8641 aaatagtgaa caatcgtgct gattcagaaa cagg
//
LOCUS       DAJTYH010000150         8582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTYH010000150 DAJTYH010000000
VERSION     DAJTYH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..254
                     /locus_tag="OO609_003629"
     CDS             <1..254
                     /locus_tag="OO609_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634898.1"
                     /translation="IHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKL
                     NVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(401..2563)
                     /gene="uvrD"
                     /locus_tag="OO609_003630"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(401..2563)
                     /gene="uvrD"
                     /locus_tag="OO609_003630"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6634899.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2647..3363)
                     /gene="yigB"
                     /locus_tag="OO609_003631"
     CDS             complement(2647..3363)
                     /gene="yigB"
                     /locus_tag="OO609_003631"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6634900.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3363..4259)
                     /gene="xerC"
                     /locus_tag="OO609_003632"
     CDS             complement(3363..4259)
                     /gene="xerC"
                     /locus_tag="OO609_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6634901.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4256..4963)
                     /locus_tag="OO609_003633"
     CDS             complement(4256..4963)
                     /locus_tag="OO609_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6634902.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4960..5784)
                     /gene="dapF"
                     /locus_tag="OO609_003634"
     CDS             complement(4960..5784)
                     /gene="dapF"
                     /locus_tag="OO609_003634"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6634903.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5821..6024)
                     /locus_tag="OO609_003635"
     CDS             complement(5821..6024)
                     /locus_tag="OO609_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6634904.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6213..7691
                     /locus_tag="OO609_003636"
                     /pseudo
     CDS             6213..7691
                     /locus_tag="OO609_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7688..>8582
                     /locus_tag="OO609_003637"
     CDS             7688..>8582
                     /locus_tag="OO609_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6634905.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT         1992 a         2356 c         2198 g         2036 t
ORIGIN      
        1 gtattcatcc caacgtcgcc tcacgtaaaa ttcgtaacgg tgttgggatt aataaacatg
       61 ggaatgccgt gtttttgttg agccagcagg cgacaaattt ttatgatttt gcctgttatg
      121 ccaaagcgaa actgaacgtt gagcagctgc tttatcttgg tggcacgatt tcacatatgt
      181 atatgaaagg cggggcaatc ccgtggcaac gctatccttt tgtcaccatg atttccgtcg
      241 agcggaaagg ttagaatgat agctttgtgt cagatactaa atatgctgct gatgccggat
      301 gcggcgtaaa cgccttatcc ggcctacatg acgttgcaat ttattgaatc tgaatgattt
      361 tttaggccaa ataaggtgcg cagcaccgca tccggcaacg ttacaccgac tccagccggg
      421 cgtaagctgc caccagccat ttgattccct ggccctggaa tgccacctgc aaacggctat
      481 gttcaccgct gccttccata ttgacgatgg tgccttcacc aaacttagcg tggcgtacac
      541 gctggccgag cttgtagccg ctgtcgttct cgaccatcgg cgtacccatc cgctgatggc
      601 tgaccgggcg gcttaccgtg gcgcgcaggc gcacctcttc cacacactct tccggcaact
      661 cgccaataaa gcgcgacggg cgatggtaaa cctctttgcc atacagacgg cgagtttccg
      721 cgtaggtcag cgtcagtttc tgcatcgcgc gggttacgcc aacgtaggcc agacggcgtt
      781 cttcttccag acgcccgcct tcatccagcg acatctggct tgggaacatg ccctcttcca
      841 taccgacgat aaacacctgc gggaactcca gacctttcgc cgagtgcatg gtcatcagtt
      901 gtaccgcatc ctgccaggta tccgcctgcc cttcgcctgc ttccagcgcc gcatgggata
      961 ggaacgcctg cagcggcatt aaatcttcgt cttcttcgtt gtagctgaac tggcgcgttg
     1021 ccgtcaccag ttcctctaag ttttcgatac gcgtctgacc tttttcgccc ttctcctgct
     1081 cgtacatggt acgcaggccg gagtctttaa ttacccggtc agtctgtaca tgcagcggca
     1141 tatcggcagt ttcctgcgct aaggcgtcga tcagttccat aaaccgctgt aaggcgctgg
     1201 cagcacgtcc ggcgagggct ttttcctgca acagttcacg acatgcctgc cagagtgtta
     1261 actggcgatc gcgcgatgtc tgacgtacca cgtccagcgt ccggtcacca ataccccgcg
     1321 ttggcgtatt caccacacgc tcaaaggccg cgtcgtcgtt acggttggca atcaggcgca
     1381 gatacgagag cgcatctttg atttcctggc gttcgaagaa gcgcatcccg ccgtaaatac
     1441 ggtacggcat actcgcctgc aacagtgctt cttccagcac acgcgactgg gcgttactgc
     1501 ggtagagaat ggcgcactcg gcaagcgccc cgccgttgtc ctgccaggtt ttgatgcggt
     1561 taaccacaaa acgcgcttca tcgagttcgt taaaagcgca atagagggaa ataggctcac
     1621 cgtccgcgcc atcggtccac agttttttac ccagacgccc gttattgttt tcaatcaggg
     1681 cgttagcggc gctcagaata ttgctggtag agcggtagtt ttgctccaga cgaataattt
     1741 cggcaccggg gaaatcatta aggaaacgct gaatattctc cacctgcgcc ccgcgccagc
     1801 cgtagattga ctggtcgtca tcaccgacga tcatcacttt gccggtgtcg cccgccagca
     1861 ggcggatcca cgcgtactga atgttgttgg tatcctggaa ttcgtccacc aggatattgg
     1921 taaaacgttc gcggtagtgt tgtaggatat gcggcttgtt aagccacagt tcgtgagcgc
     1981 gcagcagcag ctcggcgaag tccaccaggc ccgcgcgatc gcacgcttcc tgatacgcct
     2041 gatacacctt ctgccaggtc tgctccaccg gattaccgta gctttgaata tgatgcggac
     2101 gcaggccttc atctttctgg ctgttgatgt accacattgc ctggcgcggc ggccactgct
     2161 tctcgtcgag gttcatggct ttgatcagac gcttaagcag gcgtagctgg tcttcgctat
     2221 cgaggatctg gaaatcctgc ggcagattgg cgtccatatg gtgcgcacgc agcaggcggt
     2281 gcgccagccc gtggaaggtg cctacccaca tgccgccctg gctggtgccc atcagttgtc
     2341 cgatacgatg acgcatctcc gccgccgctt tgttggtaaa cgtcacagcc ataatcgagt
     2401 atggcgagca gttttccacg ctcatcagcc aggcgatacg atgcaccagt acgcgcgtct
     2461 taccactgcc cgcgcccgcc agcaccagaa ggttgctgcg tggcgcggcc accgcttcgc
     2521 gttgtttgtc attaaggctg tcgagcaggt aagaaacgtc cattggcacc gccgcgtaaa
     2581 aataggttgg gcggagaagc gcaacgccct ccgccaaaaa gctgggtata tatacagatt
     2641 tgctgattat atcagcgagg tcagagatgc caaccgggaa atttccagat gtggcagtaa
     2701 acggctgtcc caggtttgca tcagatcgcc attttccggt ctgatccaac aagcctgcat
     2761 tccgctgcga attgccccac ccacgtcagt ggtgagatcg tccccaacat gtaagatctc
     2821 gccgatcggc acgttgagtt tttccgcagc caaaaagtac atatcgctga acggttttga
     2881 gcgcccgtgc gggccagcgc gcagcacaaa ctcaaaataa tcccccaaac caaacagttc
     2941 cggctgggcg ttaccgttgg tgatcgccac cagcggccat ttcttcgcca gctgttttaa
     3001 ggtgtcgtga gtttgctgcg ggacgtcgat tcggctgcgc catttggcaa agttaatcat
     3061 tgctgcgtgt gcgcctgcac tggcttcttc ggcactcagc ccggcgtcga gcatcgcttg
     3121 ttcaatcgaa cgaaaacgcc aacgtgtcac gtcgtgataa atctcgggtt ccgcttcccg
     3181 taccgcctgg cgcaggcgtt gcagatcttc attctggaag ctgcgcagcg ccggatgata
     3241 attttgcaca aaggtaagcg cctctcgctc ggtgcgcaaa atcaccggac ggttatcgta
     3301 aagggtatca tccaggtcaa aggtgagcgc cgagatgcgc cccaaaggcc ggtaaaaacg
     3361 cattatttcc cccgtttggc gcgtggatgc gccgcatcgt acaccgaggc aaggtgttga
     3421 aaatcaagat gagtatagat ttgcgtggtg gagaggttgg catgacccag tagctcctgc
     3481 acaccacgaa gatcgccgct cgactccagc atatgcgtag cgaacgagtg acgtaattta
     3541 tgtgggtgaa cgtgattatt cagcccttgt tttatgcccc attcggcaaa gcgtttctgc
     3601 acattacgcg cggagatacg cttgcccagt ttcgacagaa aaagcgcgtc gtcttcgcta
     3661 ccaaacaggt cgcgcaaatc aagccagtgc tcaatccacg ccacagcgtt gcgaccaatc
     3721 ggcaggcggc gctctttgct gcctttcccc atcacccaca cttcgccgga ctccaggtcg
     3781 aggtgtttga tatccagccc caccagctcg gaaagacgca gacccgcgcc gtacatcact
     3841 tccagcattg cacggtcgcg tacagcgagg ggatcgttga tatcaatatc cagcagccga
     3901 ttcatatcgt cgacgtcgat atttttcggc agatgacgcg gcgctttcgg tgccgaaact
     3961 cctttcgccg ggttagcttt gagttcgttc tggctgacca gccagtcaaa aaagctacgt
     4021 agcgcagaaa gccgtaacgc cagacttgct gctcccagcc ctttacggcg actgcgtaca
     4081 gcaaaattgc gtaccatcgc tgcatcgcat tgttgccagc tttgcaggcc gttttcgctg
     4141 gcaaaattga tgatcgcctc aagctgacgc tggtagttaa gtagggttat cgggctaagc
     4201 tgacgctcca cgctcagata acgtaggtag cgttctacat cggtgtgtaa atcggtcata
     4261 cgcgttcaat ccaacgctcc agaagctccg gcaacatcag cgcaatttca tgaagtaact
     4321 gcgttccctg cccttgttga tagtgactgg catcgcgact ggtaaacagc acaacaccca
     4381 aatcagcatc gcttcccagc atcgacatcg ccaccgatcc caccgctttc gcttccggta
     4441 gcaccaccag cagctccggt ccgttaagcg gcccaagata gtgctgttcc tgccccaaac
     4501 gctgaatacg cagcggttcg aaagactgac ggcttaatgc cagatgagtg tggttcgacg
     4561 gcgcacctaa gcgccagcga tctggaaaca ggcgcagact cgcaccagct aggccgagat
     4621 cgcgcgccca gcggtgaaag cgcatcagca tatcgtcgag actgctggcg gcggtgagac
     4681 tgcgctgcag gtagagtagg cgataaaaca gcccttcgtt ggcgatagcc tgctccatca
     4741 acaacgccat gttctcttcc agaacatgaa tgtgattacg tgcgcgggcc atgtgccact
     4801 cgaccaacga aacggtgccg cgtaccggat gcggcacacg tatcgcttct actgcgcgcg
     4861 cattgcggat aaaaaactca ggatttttaa tcagataatc gacaaccgcc cggtcatcaa
     4921 gctccgtgag tgtttcctgc agttcttccc ctggttgctt catagatgaa taaatccatc
     4981 gtagacatgt accgccgggc cagtcatata taacgggtga cccggacctt tccaggcgat
     5041 atcaagacga ccgccgggga gttccacgcg tacttcttcg tccagcaaac cttgctgaat
     5101 ccctaccgcc accgccgcac acgcgccgct gccgcaggcc tgggtttctc ctgccccacg
     5161 ctcataaacg cgtaaacgaa tatgctcgcg cttaaccact tgcataaaac cgatattggc
     5221 gcgctccgga aaacgctcgt gactttccag aacaggacca agcgtttcta ccgccgcggt
     5281 atcgacatca tcgacctgaa tcacgcaatg cggatttccc atcgacacca cgccgcataa
     5341 gattgtctgc tcggcggcgc gcataatata ggtcttttcc gctttgttag cgcgaaacgg
     5401 cacggcggaa ggttcgaagt tgggttcgcc catatttacg cggaccagat catcatcggt
     5461 gacggtcaga accatccgcc cgttggcggt gctgacgcgg atatcacgct tattggtcag
     5521 tcctttcaga cgcacaaaac gggcaaagca gcgcgcaccg ttaccgcact gcgccacttc
     5581 actgccatca gcattgaaaa tgcgatagtg aaaatccagt tcaggatcat acggcggctc
     5641 aaccaccagc agctggtcaa accctacccc caggtgccga tccgccaggc gacgaatcag
     5701 ctccggtgaa aaaaagacat tctgcgttac cgcgtcgacg accataaaat cgttgccaag
     5761 gccatgcatt ttcgagaact gcatcattta ctccaatcac gcgggtacag aaactgactt
     5821 ttagtaattc acctgggatg gaccatcgcc agtggcgcga tcgtttttat ccggcaccgt
     5881 ggattgcgtt tgcgtctcta ccggtttggt cggcggcggt gcgtttttat ctgcaggcgg
     5941 gaaatagagc ggacctttca gaccgcagcc cgtcaggctg aagagagtaa gtaatacagt
     6001 gagtgcctta aacacgtttt tcattatgga ttgcctgtaa agttcatcgc tttctgcttt
     6061 ctatcatcgc aggagaaggc gtaaaagcaa gcgtttagcg actctttatg aagtctgaaa
     6121 agtgaggaaa tgctgcgagt ataacgctta ctcgtgagga tttatgttct gctggcagtt
     6181 tcgtgatcaa cacctcgcgc caggatggct ttatgcacta tcgcaatgtg gtttgtttgt
     6241 tgtcttgttc attgttttta tcgtcggcat ggggatgtcg gctttccgca cctgaacata
     6301 atatttacca gaaacaggga aaaggcgtgg tgtacctgcg cccttataaa gaaacaaatc
     6361 tttctctacc tgacgtgaat tacaagagtt tgcgtcggtt acccaattta ttaatagacc
     6421 cgacaacact agacgaatgg gataaagaac cgcctctgac tgatttgaca accgactacc
     6481 tctatgaggg cgcacaggcc tggtatcctc attattcatg gcatagcgat ggtcgcaata
     6541 tcctctacgc gggtgaagtt gtgcaaaacc ctcccggcac acctcccgtc gatgtcgcct
     6601 cgttcaaggc atggggtgat tttgccgcag ataaacacag tctctatttt gaaggcaagc
     6661 gcaccgatga caatggtggt ggaaatagct tggatatcaa aacgctgcat caggtggaat
     6721 tccggcctcc ctgggatcct gacttactgg ggctgatatt acgtgacgct aatttcttgt
     6781 atatcaatgg gcatcgcctc gctgaccccg agagtttccg tgtgctggcg cagaagtcct
     6841 gggatcagcg aggaaagttt tccaccacgt ttaacccctg cattgccgta ccatttggcc
     6901 cctgggatac cctggctcgt acacggacca aaatcctgct caacggcgaa cagcttgatg
     6961 ccgacccgga caccttttcc gtcgtgcgct ggatgcccgg ctcactcttg acctggcgtg
     7021 ataaaaacgg gctgcagcgt aaagtcctcg acaaggaaaa tctggcgtgg gatgaagatt
     7081 taacaaagca ctgtctggat ttttctctgc tggaaaagaa agtgttctgg cgtaaaggac
     7141 ctgcttgtaa acaggaagaa ttacccggac tcgatccgga gcagtttcaa cccatcagtg
     7201 atgctgtcgc ccagcatcag gactcgcttt ataccatcat cgaaacagag tctggtaacc
     7261 gtaagctgga gatcgtgaaa cttgatgatc ccaatcttat tatcaacaaa cgtttcaacg
     7321 ccgggaaacg ccacggctat ttacttacgc gtgccgaagg gtagccatac cattccggtt
     7381 tacacgtgtt tgaatctgac ggaccgctga tcttactgga taaccgttct ccggatgaac
     7441 gcgaagccca tcttaatgac catccctttt tgcgcagatg gtatgcccgc gataaccgct
     7501 acatttacag ctttgatggc gcgcagctct ggcgataccg caccgctgat ccgaaacaag
     7561 ttcgcttaat ctggaaggaa caacattcgg gatatgtcta tggcgtaaat tacaaaacgg
     7621 gatatctgga cggaaaaatt actgatgacg gcgaatttat tcctgccccg cgcaatgagg
     7681 cgacaaaatg aagggtcaaa taatcacagt tgcactcatc ctgttgggac acacatttct
     7741 gacgcctgct gtacaggcca tagggttcga ctattacaat gatcacggtg ttatgtctta
     7801 cggtaaaggt tattgggggg ataagaaaat cattgcccag tttccggaga tgagcagggc
     7861 cggtttgcgc cttgtgaaaa atatctctgg gaaaacgggt tatatacctt ccgatgaaat
     7921 aagcatagaa aatgaggaat accactgggt gacagacggc cgcgttattc tctggcgtgg
     7981 caaaatcgtt agcaatccac cgggaacact cactgtcgat attgccagct ttcaggctat
     8041 gggccgcttc gcggtcgata aatatagcct ctattttgac ggacagcgca ccgaaagtaa
     8101 tagcggcgcg tcacgtgttg agctggcgac actaaaagct atcgaaggta actctaccac
     8161 gctggtggat agtaaaaatc tctacttgtc cggtcgacgt caggggagca gcagtgatgt
     8221 tactgtatta gaaaaaagat ggtggggtat taatccacgt cttatgagtg taaacagaaa
     8281 ttcatattcc aatgatctgc ttatccgcag tgggcagaat atttatttaa atggcgttca
     8341 ccttacggcg aatgcagact catttgagat aattcgctgg atacctcatt cactgctggt
     8401 ttttcgcgac aataagggtc tgcatcgtta tccctttggt caattatcag gcaaagcgat
     8461 acccgtagat gatgacgtct cttttgaagt aggggaaagt cgcgttcgct ggcgtaaaca
     8521 gctcacgccc gaccgtcagt ggagcaagtg gatagaccta ccaggtattg aacctgaaca
     8581 at
//
LOCUS       DAJTYH010000151         8486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTYH010000151 DAJTYH010000000
VERSION     DAJTYH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(613..1809)
                     /gene="hemY"
                     /locus_tag="OO609_003638"
     CDS             complement(613..1809)
                     /gene="hemY"
                     /locus_tag="OO609_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6634906.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1812..3023)
                     /gene="hemX"
                     /locus_tag="OO609_003639"
     CDS             complement(1812..3023)
                     /gene="hemX"
                     /locus_tag="OO609_003639"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6634907.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3045..3785)
                     /gene="hemD"
                     /locus_tag="OO609_003640"
     CDS             complement(3045..3785)
                     /gene="hemD"
                     /locus_tag="OO609_003640"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6634908.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3782..4723)
                     /gene="hemC"
                     /locus_tag="OO609_003641"
     CDS             complement(3782..4723)
                     /gene="hemC"
                     /locus_tag="OO609_003641"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6634909.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5110..7656
                     /gene="cyaA"
                     /locus_tag="OO609_003642"
     CDS             5110..7656
                     /gene="cyaA"
                     /locus_tag="OO609_003642"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6634910.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7696..8016)
                     /gene="cyaY"
                     /locus_tag="OO609_003643"
     CDS             complement(7696..8016)
                     /gene="cyaY"
                     /locus_tag="OO609_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6634911.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8064..>8486)
                     /locus_tag="OO609_003644"
     CDS             complement(8064..>8486)
                     /locus_tag="OO609_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6634912.1"
                     /translation="QFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRS
                     FNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPA
                     KTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1918 a         2203 c         2182 g         2183 t
ORIGIN      
        1 ctccctgaca ggatgattac ataaataata gtgacaaaaa taaattattt atttatccag
       61 aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac ctctggcgca gttgatatgt
      121 aaggcaggtt tattatatcg cgttgattat tgatgctgtt tttagtttta atggcaatta
      181 atatatgtgt tattaattga atgaatttta tcattcataa taagtatgtg taggatcaag
      241 ctcaggttaa atattcactc aggaagttat tactcaggaa gcaaagagga ttacagaatt
      301 atctcataac aagtgttaag ggatgttatt tcccgattct ctgtggcata ataaacgagt
      361 agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg aatgacgcag
      421 agccgtttac ggtgcttatc gtccactgac agatgtcgct tatgcctcat cagacaccat
      481 ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt aacgcctgct
      541 ccgtaataag agcaggcgtt tttttatgca tcagaaaggt cccggaggtg ctttcctccg
      601 ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt aacatcaaac cgtcgcgacg
      661 catcgctgca gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa gccatgcgta
      721 atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag gcgagcgatg cttcctgcca
      781 ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac aacagcgggc gatcaccgac
      841 gtttttgatt tgctggcgta gcactttttc cagctgttcc gggttattgg ttttcagacg
      901 cgggatcggc agcagtaggc gatcgtcata ctggcgtttc aggccgtcga tgataatttg
      961 ctgggcagta tcatgatcgt cacattcaat aagatgttcc gccattgcca cctgcaacgc
     1021 tacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac cttcactacc
     1081 gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca gcattgcacg
     1141 atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca gcagagaact
     1201 ccatgcccct gtgcggatat acgcctgttc cgccagacgt aatacttcag gatggcgtgg
     1261 cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca tggttttcat tacgggccag
     1321 ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca gctccgctgc
     1381 gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg cttcggcagc
     1441 cagtagatag ttcaccaccg gttgttcagc gtgatcggca tttttcgcca tcagcttttc
     1501 aacttgctga taatcacctt ccgccagttt cagcagcgcc tgttcggtct gcttacgcgc
     1561 acggcgacgc ttacgtccga caaaccatcc acgggtgtgc gcgccagtgc ggaagatccg
     1621 ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaata tgatcgccag
     1681 gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg atcagcacat aaccctgatg
     1741 accggcaatc atcgggccaa ccactatccc cgcaatcagc aaaacaaaga gtaataacac
     1801 ttttagcatg gttattctcc ttgcggcgcg gctgccggag tatcaccttg cggtgcaggt
     1861 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtttag cttccgttgt ccccgctgcc
     1921 ggttgtgcca gcaggttacg cacgcgagtc tgcatcagtt tttccagcat cgcctggctt
     1981 tgcagggttt ccggaagatc catcgagata ttttgctggc ttaactggtc cacctcgtcg
     2041 aggaacgctt tggtggtggc atcatcagta tcgtagtaag cacgtaccca ggtggagacg
     2101 ttctccagcg cctggcgata agtctcttcc tgatgacgcg gtacagcttg tgctgcgacc
     2161 agcaggcgag agcgaatatt ttcgcgcaga tagatatcct gatttggcgc taacagcggt
     2221 acggcggtgt catcacgacg gcgaatcgta atgaagttgt ccataaagtt ctgccagctt
     2281 ttttgcagat tgatacgcca ttcgctgatg gaactggaaa gctcttcacc gtctgaatcc
     2341 atcggcgaac catcgctgtc attatcggcc agacgcaggt tatctacctg atttgaaagc
     2401 tgattaagct taaggatgat gccgtcataa tccacctgcg atactgcaga aaggctggcg
     2461 atatcatcgg taattgcccg acgaacggta atcagactcg ggtcattcat atccgccagg
     2521 ctggcgtctg cacttttcag caacgctgca gaggtcgtga cgtcctgatc gctccacagc
     2581 ttccgtccgg cgagtttcac cagaaaatcg gcctgagcca gcagccaggt tttagcatcg
     2641 ctgccggaaa tggtggcgat cttttgttgg acttcatcca actgttttgc cagcgtttct
     2701 tgctgacgat tcgcctgctt aagctgcgca gcttgttgct taataatgcc ttccagctcg
     2761 gctttttggc tctcctgggc tttttgcaat gccgtcagtt ggttagccag ggcatcgctg
     2821 gtggcggtct gattgacggc ctgttgttta ccccagccat acaaaccgac acccgccgcc
     2881 agagcaatag cgatagccac cgcgctgaga atcaatgcag tattgttctt actctttttt
     2941 tctgttgcga caggttgtga cgtggtgtcc acggcctccc tggtctcttc aaccacggcg
     3001 gaggtttttt cttgttccgt cattatggct tcctgttatg agagttattg taatgcccgt
     3061 aaaagcgcat cgttgtcagc gttatcggcg accttaatgt cttgccagcc cagttcccgg
     3121 gcgagtttcg ccaaacgctc actgacgacc aatagtcgac agtgtagtaa ccagtgctca
     3181 cgataccatt gtgggatcag cgaccagagt tgctgcaaca tttcaccgct ggtaacaacg
     3241 accatcgtca cctcgcggga ttgccagcgc atcgcttctt ctgcaccatc gtaatggatt
     3301 gcgcatcgtt gataacattc acaaaaagtg acctcagcac cgcgcaccgt cagggtatcc
     3361 ccaattagct cacgaccgcc attgccacgt aatatcagcg cacgtttgcc cgcaatattt
     3421 tgtaattcag gtaattgtag caagacttcg ctgatttccc gatcctgtgg gtagagaatc
     3481 ttctgtccgc ttacggtatg tagcgccagt gcggtggtgc gtccaatggc gaaataagta
     3541 ggtagttggg gccatttacg atcttgctga tgcagctgtg attgggcaaa agcaaccgcg
     3601 tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg ctgccagttg atcagcaagt
     3661 tgcggtaatt gtcgacccgg agaaaactca atcagcggaa aatgccaggc cacctgcccc
     3721 agtgtgcgca gacggctcac taactcttct ccagcgggag acgggcgggt gaccaggata
     3781 ctcatgccgg ggcctctccg ttatagactt cagcgaggat ctcgcgcgcg ccgttattca
     3841 gtagctcttc tgccagcgaa atccccattt gttcggcatc ttgcggcgca ccgcggcgtt
     3901 caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag cgcacatagc cagatttcgc
     3961 catcaataag ctcggcgtag ctaccaattg gcacctgaca tccgccttcg agacgggtat
     4021 tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg gtgattcagc gcggcaagca
     4081 gctcgcgagt gcgtgtatca tcaaggcggc attcaatacc gactgcacct tgtcctaccg
     4141 ccggaagaga aatctcgggt ggtaacgcgg cgcgaatgcg ggactccaga cctaaacgtt
     4201 ttagtccggc tacggcaaga atgatggcat cgtattcgcc gttatctagt ttgctcaggc
     4261 gagtgccgac gttgccgcgc agggagcgga taatcagatc cgggcggcgt tcagccagtt
     4321 ggcactggcg acgtaaactg gacgtcccga cgatactgcc tgctggtaac gcatccagac
     4381 tgtcatagtt attggacaca aaggcatcgc gaggatcttc acgctcgcaa atagtgacca
     4441 gccccagacc ttgcgggaat tcaaccggca catctttcat tgaatgtacg gcgatatcag
     4501 cgcgattctc caggagcgcg acttccagct cttttacaaa taagcctttt ccgcctactt
     4561 tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac catcggtacc agttcaacga
     4621 ccaggcccgg atggctcgcc atcaacttgt ctttgacata gtgtgcctgc cagagtgcaa
     4681 gtgggctttg gcgtgtggca attcttaaaa cattgtctaa catgcttgtt accgtcatta
     4741 tcatccgtgg tccatcctaa catccttgcc agagtgatgt cagtgttgtg gtgaaacgta
     4801 gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat cctgacaggc gtttcacgcc
     4861 gtcgtaataa ggaatttaca gagaataaac ggtgctacac ttgtatgtag cgcatctttc
     4921 tttacggtca atcagcaagg tgttaaattg atcacgtttt agaccatttt ttcgtcgtga
     4981 aactaaaaaa accaggcgcg aaaagtggta acggttacct ttgacatacg aaatatcccg
     5041 aatgccgcgt gttaccgttg atgttggcgg aatcacagtc atgacgggta gcaaatcagg
     5101 cgatacgtct tgtacctcta tattgagact ctgaaacaga gactggatgc cataaatcaa
     5161 ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat tccaacaggt ctacagtcta
     5221 ctgccgacat tgttgcacta tcaccatccg cgaatgccgg gttaccttga tggtaacgtt
     5281 cccaaaggca tttgccttta cacgcctgat gaaactcaac gccactacct gaacgagctt
     5341 gaactgtatc gtggaatgtc agtacaggat ccaccgaaag gtgagcttcc aattactggt
     5401 gtatacacca tgggcagcac ctcgtccgta gggcaaagtt gttcctctga cctggatatc
     5461 tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc agttactaca acgtaaatgc
     5521 agcctgctgg aaagctgggc cgcctcgctg ggtgtggaag tcagcttctt cctgattgat
     5581 gaaaaccgct tccgtcataa tgaaagcggc agcctggggg gcgaagattg tggctccacc
     5641 cagcatatac tgctgcttga cgaattttat cgtactgccg tgcgtctcgc cggtaagcgt
     5701 attctgtgga atatggtgcc gtgcgacgaa gaagagcatt acgacgacta tgtgatgacg
     5761 ctttacgcgc agggcgtgct gacgccaaat gaatggctgg atctcggtgg cttaagctcg
     5821 ctttctgctg aagagtactt tggtgccagc ctttggcagc tctacaagag tatcgattcc
     5881 ccatacaaag cggtgctgaa aacactgctg ctggaagcct attcctggga atacccgaac
     5941 ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg acggcgagat tgtatcgttt
     6001 ggcctcgatc catactgcat gatgctggag cgtgttactg aatacctgac ggcgattgaa
     6061 gatttcaccc gtctggattt agtacgtcgc tgcttctatt taaaagtgtg cgaaaaactc
     6121 agccgtgaac gcgcctgtgt aggctggcgt cgcgcagtgt tgagccagtt agtgagcgag
     6181 tggggttggg acgaagctcg tctggcaatg ctcgataacc gtgctaactg gaagattgat
     6241 caggtgcgtg aggcgcacaa cgagctgctc gacgcgatga tgcagagcta ccgtaatctg
     6301 atccgctttg cgcgtcgcaa taaccttagc gtctccgcca gtccgcagga tatcggcgta
     6361 ctgacgcgta aactgtatgc cgcgtttgaa gcattgccag gtaaagtgac gctggtaaac
     6421 ccgcagattt cacccgatct ctcggaaccg aatctgacct ttatttatgt gccgccgggc
     6481 cgggctaacc gttcaggttg gtatctgtat aaccgcgcgc caaatattga gtcgatcatc
     6541 agccatcagc cgctggaata taaccgttac ctgaataaac tggtggcgtg ggcatggttt
     6601 aacggcctgc tgacctcgcg cacccgtttg tatattaaag gtaacggcat tgtcgatttg
     6661 cctaagttgc aggagatggt cgccgacgtg tcgcaccatt tcccgctgcg cttacctgca
     6721 ccgacaccga aggcgctcta cagcccgtgt gagattcgcc atctggcgat tatcgttaac
     6781 ctggaatatg acccgacagc tgcgttccgc aatcaggtgg tgcattttga tttccgtaag
     6841 ctggatgtct tcagctttgg cgagaatcaa aattgcctgg taggtagcgt tgacctgctg
     6901 taccgcaact cgtggaacga agtgcgtacg ctgcacttca acggcgagca atcgatgatc
     6961 gaagccctga aaactattct cggcaaaatg catcaggacg ccgcaccgcc agatagcgtg
     7021 gaagtcttct gttatagcca gcatctgcgc ggcttaatcc gtactcgcgt gcagcaactg
     7081 gtttctgagt gtattgaact gcgcctttcc agcacccgcc aggaaaccgg gcgtttcaag
     7141 gcgctgcgcg tttctggtca aacctggggc ttgttcttcg aacgcctgaa tgtatcggta
     7201 cagaagctgg agaacgccat cgagttttat ggcgctattt cgcataacaa actgcacggc
     7261 ttgtcagtgc aggttgaaac caatcacgtc aaattaccgg cggtggtgga cggctttgcc
     7321 agcgaaggga tcatccagtt ctttttcgaa gaaacgcaag acgagaatgg ctttaatatc
     7381 tacattctcg acgaaagcaa ccgggttgag gtgtatcacc actgcgaagg cagcaaagag
     7441 gagctggtac gtgacgtcag tcgcttctac tcgtcatcgc acgaccgctt cacctacggc
     7501 tcaagcttca tcaacttcaa cctgccgcag ttctatcaga ttgtgaaggt tgatggtcgc
     7561 gaacaggtga ttccgttccg aacaaaatcg attggcaacc tgccgcccgc caatcaggat
     7621 cacgatacgc cgctattaca gcagtatttt tcgtgatgag cgtgccggaa agcgaggctt
     7681 atccggcatg caatcttagc ggaaactgac tgtttcaccc gcctgttgcg tcgccgcctg
     7741 ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa atccattcat cgcctttcag
     7801 gtcaaaatgg tagccgccct gtttggttgc cagccacacc tggtgcagcg gctcctggcg
     7861 gttaataatg attttgctgc cgttttcaaa ggtaatggtc agtacgccgc cgttgatttc
     7921 gcagtcgata tcgctgtcgc catccctgtc gtccagacgc tcttcaatgg tcagccacag
     7981 ttgatcagcc aggcgatgaa attcactgtc gttcattgtt gtatcctgtt tttaagtgat
     8041 ggcggcagta tagcggcatg gggtcagggc ttcaaagttt gcacctctgc tgctgcgttc
     8101 cggcatgatt catccgtcac cggaataatg atgtctctgt tttgaacttc acgtacgttc
     8161 tcctctctga cacgagtttt cgccgggcag ggggagggcg taatccttac ctctgtatcg
     8221 gtccatgtat aaacattgcg atcgtcataa gcgaagcgat cgtttttagt tagcgaaccg
     8281 ttaactggaa taatttgcaa gctctttgac cgtaattggc ggataaagta agcatgctgt
     8341 ttgccgctat taaatgagcg atcaatgacc aggtctggcg tatccagcgg gattatctca
     8401 agctggtctt caccaaatgt cgataatttt gttacataca gccgatcttt atattgcgca
     8461 atattgccag taatcagatg gaattg
//
LOCUS       DAJTYH010000152         8332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTYH010000152 DAJTYH010000000
VERSION     DAJTYH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..157
                     /locus_tag="OO609_003645"
     CDS             <1..157
                     /locus_tag="OO609_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634913.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO609_003646"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="OO609_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6634914.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO609_003647"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="OO609_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6634915.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1244
                     /locus_tag="OO609_003648"
     CDS             873..1244
                     /locus_tag="OO609_003648"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6634916.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1265..6126
                     /gene="traI"
                     /locus_tag="OO609_003649"
                     /pseudo
     CDS             1265..6126
                     /gene="traI"
                     /locus_tag="OO609_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6146..6892
                     /gene="traX"
                     /locus_tag="OO609_003650"
     CDS             6146..6892
                     /gene="traX"
                     /locus_tag="OO609_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6634917.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6947..7507
                     /locus_tag="OO609_003651"
     CDS             6947..7507
                     /locus_tag="OO609_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6634918.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7638..7850
                     /locus_tag="OO609_003652"
     CDS             7638..7850
                     /locus_tag="OO609_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6634919.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8094..>8332
                     /locus_tag="OO609_003653"
     CDS             8094..>8332
                     /locus_tag="OO609_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6634920.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2671 g         1662 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cttgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcag gtggaggcca gtgcctccac ctgacggacg gacagtcaga
     1261 aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca gtcgcgatca
     1321 ggaaccacag ttacacacgc atgcggtggt gactaatgtt acgcagtata acggcgaatg
     1381 gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg tgtacgctaa
     1441 tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg aggcgctggg
     1501 ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggtg taccggtgga
     1561 ggccttttcc ggacgctcac agactatccg ggaggccgtc ggggaagacg cctcgctgaa
     1621 atcccgggat gtggcggcgc tggatacgcg taaatccaaa cagcacgtcg atcctgaagt
     1681 cagaatggcc gagtggatgc agacgctgaa ggaaaccggg ttcgacatcc gggcatatcg
     1741 tgacgcagcg gaacaacggg catatacccg cacgcagaca cccggacccg cttcacagga
     1801 cgggccggat gtgcagcagg cggtgacaca ggccattgcc ggattaagtg aacgcaaagt
     1861 gcagtttatg tatacggacc tgctggccag gacggtcggc atactgccgc cggaaaatgg
     1921 tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agccgtgagc agcttatccc
     1981 cctcgatcgt gagaaggggc tgttcacgtt cgggattcat atgctcgatg agctgtcagt
     2041 ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc cggagaaaag
     2101 tgtcccccgg acggccggtt acagcgatgc cgtcagtgtt ctggcacagg accgtccgtc
     2161 gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg tggctgaact
     2221 ggtcatgatg gcccgggagc aggggcggga ggtgcagatt atcgctgctg accgtcgctc
     2281 gcagatgaac ctgaagcagg atgaacggct gtccggtgaa ctgataaccg gacgtcgtca
     2341 gttgctggaa ggaatggcct tcacgccggg cagtaccgtt atcgtcgacc agggcgaaaa
     2401 actctccctg aaagagacgt taaccctgct ggatggtgcc gcacgtcata acgtacaggt
     2461 cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcactgatgg ccatgaagga
     2521 tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca ccatcatcag
     2581 tgaaccggac cgtaatgtcg gctggccgga gattttgcgg ccagcgtgaa agccggagaa
     2641 gagagcgtgg cacaggtcag cggagtacgg gaacaggcca tactgacaca ggccattcgc
     2701 agtgagctga aaacacaggg cgtgctcgga cacccggagg taaccatgac cgccctgtca
     2761 ccggtctggc tggacagccg gagccgttat ctgcgggata tgtaccgccc gggaatggtg
     2821 atggagcagt ggaacccgga gacacgcagt catgaccgct atgtgacaga acgtgtgacg
     2881 gcgcagagtc acagcctgac cctgcggaat gcgcagggcg aaacgcaggt ggtgcgtatt
     2941 tcttccctgg acagcagctg gtcgctgttc cggccggaaa aaatgccggt ggcagacggt
     3001 gagcgtctga gggtgacagg gaaaattccc ggactccgcg tctccggcgg tgaccgcctg
     3061 caggtggcat ccgtcagtga agatgcgatg acggttgttg tgccgggacg ggctgaaccg
     3121 gccaccctgc ctgtgagcga ttcaccgttc actgcactga agctggagaa cggctgggtg
     3181 gaaacgcccg ggcattccgt cagcgacagt gcgacggttt ttgcctccgt cacacagatg
     3241 gcaatggaca atgccaccct gaatggtctg gcccgcagtg gtcgtgatgt ccggctgtat
     3301 tcctcactgg atgaaacccg tactgcggaa aaacttgccc gccatccctc ctttacggtg
     3361 gtttctgagc agataaaggc gcgtgccggt gagacattgc tggaaaccgc tatcagtctg
     3421 cagaaagccg ggcttcacac gccggcacag caggccattc atctggccct tcctgtgctg
     3481 gaaagtaaaa acctggcctt cagcatggtg gacctgctga cagaggcgaa gtcgtttgct
     3541 gcagaaggaa ccggttttgc tgacctggga ggggaaatca atgcgcagat aaaacggggt
     3601 gatttactgt atgtggatgt ggcaaaaggc tatggcacag gcctgctggt ttcccgtgcg
     3661 tcgtatgagg cagaaaagag cattcttcgc catattctcg aaggtaagga ggcggtcacg
     3721 ccgctgatgg agagagtacc tggcgaactc atggagaaac tgacatcagg acagcgcgcc
     3781 gccacccgta tgatactgga aacgtctgac cgtttcacgg tggtgcaggg ctatgccggt
     3841 gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg tgaacatgct gccggagagt
     3901 gaacgtcccc gtgtcgtggg gctggggccc acacaccgtg cggtcgggga gatgcgcagc
     3961 gccggcgtgg atgcacagac actggcgtcc tttctgcatg acacgcagct gcagcagcgc
     4021 agcggagaaa ctccggattt cagcaacacg ctgttccttc tcgatgagag ctctatggtg
     4081 ggcaataccg acatggcacg ggcatacgcc ctgattgcgg ccggtggcgg tcgggctgtg
     4141 gccagcggtg acacggacca gctgcaggcc atcgcgcccg gtcagccttt ccgtctccag
     4201 cagacgcgca gtgctgccga tgtggtcatc atgaaggaga ttgtgcgtca gacgccggaa
     4261 ctgcgggagg cggtatacag cctgattaac cgggatgtgg aaagggcgct gtccgggctt
     4321 gagcgtgtga aaccgtctca ggtgccacgt ctggagggcg catgggcacc ggagcactcc
     4381 gtgacggagt tcagtcacag ccaggaagcg aaactggcag aagcgcagca gaaggcgatg
     4441 ctgaaaggcg aggcttttcc ggatgtcccc atgacactgt atgaagccat tgtccgcgac
     4501 tataccggca ggacaccgga agcacgggag cagacgctga ttgtcacgca cctgaatgag
     4561 gaccggcgcg tactgaacag catgattcat gatgcacggg aaaaggccgg agaactgggg
     4621 aaggtgcagg tcatggtgcc tgtcctgaac acagcgaata tacgcgacgg ggagctgcgt
     4681 cgtctctcca cctgggagaa taacccggat gcccttgccc tggtggataa tgtgtatcac
     4741 cggattgccg gtatcagtaa ggatgacggg ctgataaccc tgcaggatgc ggaaggtaac
     4801 acgcggctga tttcgccccg ggaggcggtg gctgaaggcg tcacgctgta caccccggac
     4861 accatccggg tggggaccgg tgacaggata cgcttcacga agagtgaccg ggagcgcggt
     4921 tatgtggcca acagcgtctg gacggtgaca gcggtttccg gtgacagtgt cacactttct
     4981 gacggtcagc agacccgtgt gattcgcccc ggtcaggagc gggcagagca acatattgac
     5041 ctggcctatg ccatcaccgc ccacggtgca cagggggcga gtgaaacctt tgccatcgcg
     5101 cttgaaggca cggaaggtaa ccggaaactg atggccggct ttgagtcagc ctacgtggcc
     5161 ctgtcgcgta tgaagcagca tgtgcaggtg tacaccgata accgtcaggg ctggacggat
     5221 gccatcaaca atgccgtaca gaaaggaact gcccacgatg tgtttgagcc gaaaccggac
     5281 cgggaggtca tgaatgcaga gcggctgttc agtacggcgc gggagctgcg ggacgtggcg
     5341 gcaggccgtg ccgttctccg tcaggcgggg ctggccgggg gagacagtcc tgcacggttt
     5401 attgctccgg gacgtaaata tccgcaaccg tatgtggcac tgccggcgtt tgaccgtaac
     5461 ggcaagtccg ccggtatctg gctgaacccg ctgaccacgg atgacggaaa cgggctgcgg
     5521 ggattcagcg gtgaaggtcg ggtaaaaggc agcggggatg cgcagttcgt ggccctgcag
     5581 ggcagccgta acggagaaag cctgctggct gataatatgc aggatggcgt ccggattgcc
     5641 cgtgataatc ctgacagtgg tgtggtggta agaatcgccg gtgaaggccg tccgtggaat
     5701 cccggtgcca tcaccggtgg ccgcgtgtgg ggggatatcc cggacaacag cgtccagccg
     5761 ggagccggaa atggtgaacc ggtcacggca gaggtactgg cacagcggca ggctgaagag
     5821 gccatccgcc gtgaaacgga acgtcgcgca gatgaaattg tccgtaaaat ggcagagaac
     5881 aaacctgacc tgccggacgg caaaacagag caggctgtca gggaaattgc cgggcaggag
     5941 cgtgaccggg ctgccataac tgaacgggaa gccgcgctgc cggagagtgt actgcgtgaa
     6001 ccacaacggg tgcgggaagc ggtccgggaa gttgcccggg aaaatttgct gcaggagcgt
     6061 ctgcagcaga tggagcggga tatggttcgt gacctgcaga aagagaaaac cccgggcgga
     6121 gactgataca ggaagacaaa cactgatgac aacggataac acgaacacga cacgtaacga
     6181 ttcactggct gcacggaccg atacctggtt gcagtcattt ctggtctggt cacccggaca
     6241 gcgggatatc atcaaaacgg tggcactggt gctgatggta ctggaccaca tcaatctgat
     6301 attccagctg aagcaggaat ggatgtttct ggccgggcga ggagcctttc cgctttttgc
     6361 cctggtgtgg gggctgaatc tgtcccgtca tgcgcatatc cggcaaccgg ccattaaccg
     6421 gctgtgggga tggggaatta ttgcccagtt cgcatattat ctggcaggct ttccttggta
     6481 tgaggggaat atcctgtttg cctttgcagt ggcggcacag gtgctgacgt ggtgtgaaac
     6541 gcgcagtggg tggcgtacag cagcggccat actcctgatg gccctgtggg ggcctttgtc
     6601 cggcaccagt tacggtattg ccgggttgct gatgctggca gtcagcaacc ggctgtaccg
     6661 ggcggaagac agagcggaac gtctggcgct ggtcgcctgc ctgctggctg ttattccggc
     6721 gcttaacctt gccaccagtg atgcagcggc ggtggccggt cttgtgatga cggtgctgac
     6781 cgttggtctg gtgttgtgtg ccggaaaatc attaccccgt ttctggcccg gagacttttt
     6841 cccgacgttc tatgcctgtc atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg
     6901 gtatctttgg ccgcaagagg atgattcgtc agaggcagaa cacagcatga cagagcagaa
     6961 gcgaccggta ctgacactga agcggaagac agagggaaca gcgcctgtcc gcagccggaa
     7021 aaccatcatc aatgtcacca cgccaccaaa gtggaaggtg aaaaagcaga agctggccga
     7081 gaaagcggcc cgtgaagcag agctggcggc aaaaaaagcg caggccagac aggcgctgtc
     7141 catttatctg aacctgccca cgctggatga ggctgtgaat accctgaagc cctggtggcc
     7201 gggattattt gacggtgaca cgccccggct tctggcctgc ggtatccggg acgtgttact
     7261 ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa aaactgcgca gggcgctgaa
     7321 ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa gccggtgcct gccggtatga
     7381 cacggaaggg tatgtgacgg agcatatttc tcaggaggag gaagcgtatg cgggagcgcg
     7441 tctggcgaaa attcgtcgcc agaaccggat aaaggcagaa cttcaggccg tgcttgatga
     7501 gaaataaaaa aagcctcccc cagagagagg aggcagggaa ataaggctta aaaggaaata
     7561 acttccgctt ccgaaaggaa gccagtccac tgaattattt atcacaataa tactgacaac
     7621 taaaggggga caagaaaatg aatggattca gaaacagttc acggaacggc caggtctggc
     7681 gttaccagcg tgccggcggg cgggccgtta ttctggaagt cagtggacgc tggatggaag
     7741 cggcagaagc atggcgacgg gctgcctgtg ttgcgcctag gacagactgg cagcagtttg
     7801 cccggaaaag ggctgaacac tgccaccggc gctgccgggg aagggtgtaa cgaaaaaagc
     7861 agccttcatt actctaggag tcagaaggct gaaaggagtt tatgagattt gcttcttaat
     7921 atccggtcgg gcagtatgac cggacagttc tgagtagcac agagtctttt ccgcattaag
     7981 tcgatttttt atattttgtg attagtttca caaaataagg tgttattcaa tgtgtgctgc
     8041 actattcagg ggggctgaaa tacaggtggc atttatctga tatggagaat aacatgagga
     8101 aatatattcc actggcatta tttatctttt catggccggt attgtctgca gatattcatg
     8161 gacgggttgt tcgtgtactg gacggtgata ccatcgaggt catggactca ctgaaggcgg
     8221 tgcggatcag gctggtaaat attgatgcac cggaaaagaa acaggattat ggccggtggt
     8281 caacagacat gatgaaatca ctggtcgcag gaaaaacggt taccgtcaca ta
//
LOCUS       DAJTYH010000153         8111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTYH010000153 DAJTYH010000000
VERSION     DAJTYH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            12..296
                     /locus_tag="OO609_003654"
     CDS             12..296
                     /locus_tag="OO609_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6634921.1"
                     /translation="MLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDVSTQSSPVEM
                     NCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEYN"
     gene            391..924
                     /gene="ymdB"
                     /locus_tag="OO609_003655"
     CDS             391..924
                     /gene="ymdB"
                     /locus_tag="OO609_003655"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6634922.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            866..2347
                     /gene="clsC"
                     /locus_tag="OO609_003656"
     CDS             866..2347
                     /gene="clsC"
                     /locus_tag="OO609_003656"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6634923.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2355..3512)
                     /gene="mdoC"
                     /locus_tag="OO609_003657"
     CDS             complement(2355..3512)
                     /gene="mdoC"
                     /locus_tag="OO609_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6634924.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3906..5441
                     /gene="mdoG"
                     /locus_tag="OO609_003658"
     CDS             3906..5441
                     /gene="mdoG"
                     /locus_tag="OO609_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6634925.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPLPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5434..7977
                     /gene="mdoH"
                     /locus_tag="OO609_003659"
     CDS             5434..7977
                     /gene="mdoH"
                     /locus_tag="OO609_003659"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6634926.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2029 a         1936 c         2129 g         2017 t
ORIGIN      
        1 tagactctct tatgctcggc agtttgtttt ttccttttat tgccattgca ggaagcaccg
       61 cgcaaggggg cgtgatccat ttttatggcc aaattgtgga accggcatgt gacgtcagca
      121 cccagtcatc acccgtagaa atgaactgcc cacagaatgg ttctattccg ggcaaaacct
      181 actccagcaa agcgttaatg agcggcaatg tcaaaaacgc gcaaatagcc tcagtaaaag
      241 tgcagtatct tgataaacag aaaaagctgg ctgtaatgaa catcgaatat aactaagttt
      301 ctgaacaact cacgttgctg agcagaaaaa tgcgatttaa ccaaaaagcc tgctgtacac
      361 ttaagaaaca agaaggtgta aggaggcgtt atgaaaacgc gtattcatgt tgtgcagggt
      421 gatattacca aactggccgt tgatgtgatt gtgaatgcgg ctaatccgtc attaatggga
      481 ggcggcggcg tcgatggggc cattcatcgc gcagcgggtc cggccctgct ggatgcctgt
      541 ttaaaagtca ggcaacagca gggcgattgc cctacggggc atgccgttat tacgcttgca
      601 ggcgatcttc ccgctaaagc cgtagtacac accgtcgggc cagtctggcg tggtggggaa
      661 caaaacgaag accagctttt gcaggatgcc tatctgaata gcctacgact ggtggcggca
      721 aacagctata cgtcagtggc ttttcctgca atcagtactg gggtttatag ttaccctcgt
      781 gcggcagcgg ctgaaatcgc agttaaaacc gtttcagaat ttattacccg tcacgcttta
      841 cccgaacagg tatactttgt ctgttatgat gaagaaaacg cccacctcta cgaaagactc
      901 cttacccaac aaggagatga atgatttgcc ccggctggcg agcgcggtgc tgccactgtg
      961 ttcgcaacat cccggtcagt gtggtctttt ccctctggag aaaagtctgg atgcgtttgc
     1021 cgcccggtat cgtctggccg aaatggcaga gcatacgctg gatgttcagt attacatctg
     1081 gcaggacgat atgtcggggc ggttactgtt ttccgccctg ttagccgcag caaagcgtgg
     1141 cgttcgcgtc cgtttgttgc tggacgacaa caatacgcct ggacttgacg acattttgcg
     1201 cttgcttgac agtcatccac gcattgaagt ccggcttttt aatcctttct cgtttcgctt
     1261 gctacgtccg cttggatata tcaccgactt ttcccgtctc aatcgccgga tgcacaataa
     1321 aagtttcact gtcgatggcg tggttaccct agtgggagga cgaaatattg gtgatgccta
     1381 ttttggtgca ggggaagagc cacttttttc ggatttagat gtcatggcaa taggccccgt
     1441 ggtagaggat gttgccgatg atttcgcccg ctactggtat tgcaaatcgg tttcaccctt
     1501 acagcaggtg ctggatgtcc cggaaggtga aatggcggat cgcatcgagt tacccgcctc
     1561 ctggcataac gatgccatga cgcatcgtta tttacgcaaa atggaatcca gtccatttat
     1621 aaatcatctg gttgatggaa cgttgccgct tatctgggcg aagacacgtt tattaagtga
     1681 tgatccggcg aaaggggagg gcaaggcaaa acggcattca ctgttaccgc agcgcctgtt
     1741 cgatatcatg ggctcaccca gtgaacgcat cgatattatc tcttcctatt ttgtaccgac
     1801 acgcgcaggt gtggcgcaac tcttacggat ggtgagaaaa ggggtaaaga ttgcgatcct
     1861 aaccaattct cttgccgcta acgatgttgc tgtcgtccat gccggatacg cgcgctggcg
     1921 caaaaaattg ctccgctatg gcgtggaatt atatgaactc aagccgacgc gtgaacaaag
     1981 cagtacgtta cacgatcgcg gcataaccgg taattccggt gccagcctgc atgctaaaac
     2041 ttttagcatc gatggtaaaa cggtgtttat cggctctttc aatttcgatc cgcgttcaac
     2101 attgctcaat actgaaatgg gcttcgtgat agagagcgaa acgctggcac agttaattga
     2161 taaacgcttt attcagagcc agtacgatgc ggcctggcag ctccgtctgg acaggtgggg
     2221 acggatcaac tgggttgatc gtcatgcaaa gaaagaggtt attctcaaaa aagaacccgc
     2281 caccagtttc tggaagcggg ttatggtcag actggcgtcg atattgcccg tggaatggtt
     2341 attgtaaatg tggcttaacg ggctggtgct ttatcgttct cacgcttaac aaccggttta
     2401 ccagagaaca aaaacttcag taacgggatg cgtaaatgaa tttcatacag aattatcgca
     2461 atccctacta cgaatatcag gccacagaga aaaccaagcc agttggaggt gatgtgcggt
     2521 gtaatgtatg cgccgaaaaa cagcgttaac gggtggtgaa ccagatagat aaacagcgat
     2581 gcgttaacaa aataagtcac ccgcgctgac tggaagttaa gcaaacggtg gccaaaggag
     2641 aagaccacat tcaccatcca caggccgagg accatggtga tcaccgactc ggtttcgtac
     2701 atccaggcat cgccactgcc atagcgctgg ttgagtaaat aagcgacaaa cgccaatgct
     2761 gcagcgaggg tacagccacg agacggcgtg gtaaacaagg ctttaagatg agggaaaatg
     2821 aaagccagtg cgccgaggat aaagaacggt aaataaaata gcgtttgcat gacaataaaa
     2881 ttgaacatgc cattactcag aatgggtgga tacacaataa aaatcgttct tcttattacc
     2941 gcatagccga tgccgaggca taaaaaaatc accgatagtt ttaccatcga gaatttttta
     3001 ttcgttttat cagaattttc taaattattt ctgatgcgct taaatatcca tacacacagc
     3061 gtcgtcatga ctactaacac cagtaaaaac cacaggtgtg atattaattc ccaggccaac
     3121 gtattatatt tgtcatacaa tgatagccca ggccaacttt ccgctttccc tttgacatat
     3181 tgcagcataa taaattgcgg taatgtcagt agggggatgg ctgttaacat cgggatacct
     3241 acacgttcga cacgtacttt ccaccatttt ttcaagggat agcgtaaaaa aagcatgtag
     3301 gaaaagtatc cggatataac gaaaaatacc tgcatgcgga acgagtggat gaagtcatta
     3361 aaaagggtta gccacaatga cggttcggcg ctattcacat gccatgtatg gctcgaatag
     3421 attaaagaaa tatgaaaagg gatccctaac aacatcagcc aggcgcggat ggagtcgagg
     3481 aaatattcac gttgcgcggg tactggattc atatatggtt aactaatctc ggatttttcg
     3541 tcttatccct gtcgggttat gcctttaggc ttgttgccat agcgacaccg acctgaccgc
     3601 gccaggcgca ggcttcaagg tttttatgca tagcatcatc gctaccacta accagaatgg
     3661 aagcgtctgt aagacggttg ataaataaat ttgctggcaa accctacacg aagtcgatgc
     3721 ttctgtcttt aggagaagca cggaaagtga aaacggttgc aatcaggtgc ttaatccatg
     3781 agccagcgtg ctgaacgata ccgggattca gttgtcggaa tggctggtta tccattaaaa
     3841 tagatcggat cgatataagc acacaaaggg ggaagtgctt actaattatg aaacataaac
     3901 tacaaatgat gaaaatgcgt tggttgagtg ctgcagtaat gttaaccctg tatacatctt
     3961 caagctgggc tttcagtatt gatgatgtcg caaagcaagc tcaatcctta gccgggaaag
     4021 gctatgaggc gcctaaaagc aacttgccct ccgttttccg cgacatgaaa tacgcggact
     4081 atcagcagat ccagtttaat catgacaaag cgtactggaa caatctgaag accccattca
     4141 aactcgagtt ctaccatcag ggtatgtact tcgatacccc ggtcaaaata aatgaagtga
     4201 ctgccaccgc agtcaaacga atcaaataca gcccggatta tttcactttc ggcgatgttc
     4261 agcatgacaa agacacggta aaagaccttg gttttgccgg ttttaaagtg ctttacccga
     4321 tcaacagcaa agataaaaac gatgaaatcg tcagcatgct cggggccagc tatttccgcg
     4381 tgattggtgc aggtcaggtt tatggccttt ctgcccgcgg cctagcaatt gataccgctt
     4441 tgccatcggg tgaagaattt ccacgcttca aagagttctg gatcgagcgt ccaaaaccga
     4501 ctgataaacg tttaaccatt tatgcattgc ttgactcgcc gcgtgcgaca ggtgcttaca
     4561 aattcgtggt tatgccaggg cgtgacacgg ttgtggatgt gcagtcgaaa atctatctgc
     4621 gcgataaagt cggcaaactg ggggttgcac cgttaaccag tatgttcctg tttgggccga
     4681 accaaccgtt gcctgcaaat aactatcgtc cggagttgca cgactctaac ggtctgtcta
     4741 tccatgctgg taatggcgaa tggatctggc gtccgttgaa taacccgaaa catttagcgg
     4801 tcagcagctt ctccatggaa aacccgcgag gctttggtct gttgcaacga ggtcgtgatt
     4861 tctcccgctt tgaagatctc gatgatcgtt acgatctccg tccaagcgca tgggtgactc
     4921 cgaaagggga gtggggcaaa ggcagcgttg agctggtgga aattccaacc aacgatgaaa
     4981 ccaacgataa catcgtcgct tactggacgc cggatcagct gccggagccg ggtaaagaga
     5041 tgaactttaa atacaccatc accttcagcc gtgatgaaga caaactgcat gcgccagata
     5101 acgcatgggt gcaacaaacg cgtcgttcaa cgggggatgt gaagcagtcg aacctgattc
     5161 gccagcctga cggtactatc gcctttgtgg tcgattttac cggcgcagag atgaaaaaac
     5221 tgccagagga taccccggtc acagcgcaaa ccagcattgg tgataatggt gagatagttg
     5281 aaagcacggt gcgctataac ccggttacca aaggctggcg tctggtgatg cgtgtgaaag
     5341 tgaaagatgc caagaaaatc actgaaatgc gtgctgcgct ggtgaatgcc gatcagacgt
     5401 tgagtgaaac ctggagctac cagttacctg ccaatgaata agacaactga gtacattgac
     5461 gcaatgccca tcgccgcaag cgagaaagca gcattgccga agactgatat ccgcgccgtt
     5521 catcaggcgc tggatgccga acaccgcacc tgggcgcggg aggatgattc cccgcaaggc
     5581 tcggtaaagg cgcgtctgga acaagcctgg ccagattcac ttgctgatgg acagttaatt
     5641 aaagacgacg aagggcgcga tcagcttaag gcgatgccag aagcaaaacg ctcctcgatg
     5701 tttcccgacc cgtggcgtac caacccggta ggccgtttct gggatcgcct gcgtggacgc
     5761 gatgtcacgc cgcgctatct ggctcgtttg accaaagaag agcaggagag cgagcaaaag
     5821 tggcgtaccg ttggtaccat ccgccgttac attctgttga tcctgacgct cgcgcaaact
     5881 gttgtcgcga cctggtatat gaagaccatt cttccttatc agggttgggc gctgattaat
     5941 cctatggata tggttggtca ggatttgtgg gtttccttta tgcagcttct gccttatatg
     6001 ctgcaaaccg gtatcctgat cctttttgcg gtactgttct gttgggtgtc cgccggattc
     6061 tggacggcgt tgatgggctt cctgcaactg cttattggtc gcgataaata cagtatatct
     6121 gcgtcaacag ttggcgatga accattaaac ccggagcatc gcacggcgtt gatcatgcct
     6181 atctgtaacg aagacgtgaa ccgtgttttt gctggcttgc gtgcaacgtg ggaatcagta
     6241 aaagccaccg ggaatgccaa acactttgat gtctacattc tgagtgacag ttataacccg
     6301 gatatctgcg tcgcagagca aaaagcctgg atggagctta tcgctgaagt cggtggcgaa
     6361 ggtcagattt tctatcgccg ccgccgtcgc cgcgtgaagc gtaaaagcgg taatatcgat
     6421 gacttctgcc gtcgctgggg cagccagtac agctacatgg tggtgctgga tgctgactcg
     6481 gtaatgaccg gtgattgttt atgcggcctg gtgcgcttga tggaagccaa cccgaacgcc
     6541 gggatcattc agtcgtcgcc gaaagcgtcc ggtatggata cgctgtatgc gcgctgtcag
     6601 cagttcgcga cccgcgtgta tgggccactg tttacagccg gtttgcactt ctggcaactt
     6661 ggcgagtcgc actactgggg acataacgcg attatccgcg tgaaaccatt tatcgagcac
     6721 tgcgcactgg ctccgctgcc gggcgaaggt tcctttgccg gttcaatcct gtcacatgac
     6781 ttcgtagaag cggcgttgat gcgccgtgca ggttgggggg tctggattgc ttacgatctc
     6841 ccgggttctt atgaagaatt gccgcctaac ttgcttgatg agctaaaacg tgaccgccgc
     6901 tggtgccacg gtaacctgat gaacttccgt ctgttcctgg ttaagggtat gcacccggtt
     6961 caccgtgcgg tgttcctgac gggcgtgatg tcttatctct ccgctccgct gtggtttatg
     7021 ttcctcgcgc tctctactgc attgcaggta gtgcatgcgt tgaccgaacc gcaatacttc
     7081 ctgcaaccac ggcagttgtt cccggtgtgg ccgcagtggc gtcctgagct ggcgattgca
     7141 ctttttgctt cgaccatggt gctgttgttc ctgccgaagt tattgagcat tttgcttatc
     7201 tggtgcaaag gaacgaaaga atacggcggc ttctggcgcg ttacattatc gttgctgctg
     7261 gaagtgctgt tttccgtgct gctggctccg gtacgcatgc tgttccatac ggtcttcgtt
     7321 gtcagcgcgt tccttggctg ggaagtggtg tggaattcac cgcagcgtga tgatgactcc
     7381 acttcatggg gcgaagcgtt caaacgccac ggctcacagc tgctgttggg gttagtgtgg
     7441 gctgttggga tggcgtggct ggatctgcgt ttcctgttct ggctggcacc gattgtcttc
     7501 tcgttgatcc tgtcaccgtt tgtttcggtg atttccagcc gtgccaccgt tggtctgcgc
     7561 accaaacgct ggaaactgtt cctgatcccg gaagagtatt caccgccgca ggtgctggtt
     7621 gataccgatc ggttccttga gatgaatcgc caacgctccc ttgacgatgg ctttatgcac
     7681 gcagtgttta acccgtcatt taacgctctg gcaaccgcaa tggcgacagc gcgtcaccgc
     7741 gccagtaagg tgctggaaat cgcccgtgac cgccacgttg aacaggcgct taacgagacg
     7801 ccagagaagc tgaatcgcga tcgtcgcctg gtgctgctaa gcgatccggt gacgatggcc
     7861 cgtctgcatt tccgcgtctg gaattcccct gaaagatatt cttcatgggt gagttattac
     7921 gaagggataa agctcaatcc actggcattg cgtaaaccgg atgcggcttc gcaataaaaa
     7981 cgtagttgcc tgatgcgcta cgcttatcag gcctacatcg ttcctgcaat ttattgattt
     8041 tgcaagattt tgtaggtcgg ataaggcgtt cacgccacat ccggcaaaaa caacgagcca
     8101 ataaaaatac c
//
LOCUS       DAJTYH010000154         8074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTYH010000154 DAJTYH010000000
VERSION     DAJTYH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..248)
                     /locus_tag="OO609_003660"
     CDS             complement(<1..248)
                     /locus_tag="OO609_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6634927.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="OO609_003661"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="OO609_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="OO609_003662"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="OO609_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="OO609_003663"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="OO609_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO609_003664"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO609_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6634928.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="OO609_003665"
     CDS             complement(2964..3278)
                     /locus_tag="OO609_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634929.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="OO609_003666"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="OO609_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO609_003667"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO609_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6634930.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="OO609_003668"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="OO609_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="OO609_003669"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="OO609_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5945..6104)
                     /locus_tag="OO609_003670"
                     /pseudo
     CDS             complement(5945..6104)
                     /locus_tag="OO609_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6108..7337)
                     /locus_tag="OO609_003671"
                     /pseudo
     CDS             complement(6108..7337)
                     /locus_tag="OO609_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO609_003672"
     CDS             complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO609_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6634931.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7704..>8074)
                     /locus_tag="OO609_003673"
     CDS             complement(7704..>8074)
                     /locus_tag="OO609_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6634932.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
BASE COUNT         2068 a         1882 c         1773 g         2351 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaatac
     5581 atcacctaaa atagtattgt tttttatggt tggttgaagg tcgtggtgat gatgttagtc
     5641 cttttgagaa tattccagtg ccagatctta cttataataa aggggcttgg cagtttggat
     5701 aaactattgt tcttattcca ctaatacaaa ctttttgtaa agtatattaa atatacttca
     5761 tttcatactg aaatccccgg aagtattcaa tcaaacaatt acaccataaa aggaagaggg
     5821 ggccctaatt aagttgtcaa gcatgtttat gatacctgca gggtataaat tttctggctg
     5881 caagcagaca catataaaac gtctttattg atcctaccca cataatatgg acacagtcgt
     5941 aagtgaggct ctggttttca aattgttccg gactgagacc accacaccaa cggtgccgtc
     6001 gccagcgatt gtaatcgcac tcgatataac taaacactgt tgcccgcact atttcccgat
     6061 ggataatagt gttctccgtg gagacattcc attttcagcg aatgaatttt catctgttgc
     6121 tgtatccagt catacagtga ctgcatcagt ggcgcggctc tggcttttct tgccgccaga
     6181 cgctgttctg ctgaacatcc ccggacttct gcctcgatgg catacagttc accgatacgc
     6241 tgcagggctt ccgtggtgat gtcggttggc actcttgcat gcacatcgtg gatttttctc
     6301 cggacatgag ccatacatgc ggcttccgtt attctgccgg attcgtacaa cgcccggtaa
     6361 ccaccgtaag catcggcctg aagcactccg ctgtaaccgg ccagatgatt ctgtggatgg
     6421 atacctttcc ggtccggtga gtacgcgaac cagaccgccg gaggcatttc cgaaccggcg
     6481 ttacggtcat cacgaacgta gacccacagc cgggcagtcc ttgttttacc gctgcccggc
     6541 tcctggaccg gaacggggat atcatcggca tggactttac cgggcatcag cacatactgg
     6601 cgcaggatgt catacagcgg ttccagcagt tcagcaacag cacctgtcca gcgccccagt
     6661 gtggcacggc tcagctccac gccctgacga cggtatattt ctgactggcg gtataacggc
     6721 agatagtctg catattttcc agcaacaaca tgggccagaa gccccgctcc ggcataactg
     6781 cgtgcaatgg gttttgaagg caccggtgcc tgcacgatat ggtcacaccg gcaacaggcc
     6841 agtttcggac gttgtgtttc gataacctta aaggcgctgc tgataagctc cagctgctct
     6901 gacacatcac atcccagaga actgagttcg ccaccacagg ccgggcagca ttcctcttcc
     6961 ggacggatga cccgggtttc acggggaagt gaggctggta acggtttacg ggctgaagac
     7021 tggcgcaggg gagatggcag taccgggtca tattgctcac ccagcgtttc cgccatttct
     7081 tcctgaagtg cgctgattcg ctcctgtgct tccagtatct gccgttcggt ttttgcgcga
     7141 agtttttctg agcttttacc gaactgcatg cgctgcagtt tcgcaaccag cgccttcagc
     7201 cggttgattt cggaagcata agccgccacc cgctgtgaga gcaggcggtt gtatttcgcc
     7261 atctggcgga tggtgtcctg ttgcgtctgc aacagtgccc gcaggcgggc attctcatga
     7321 gcaagtgagg tgtccatatc ctcactttac aacgggttat atgcggatgc cagcgcgttc
     7381 cgttcgtttc gggtgtttcc agtcgatgcc ctcaagaagc atggataact gggccggagt
     7441 aaggtgcacc ttgccgtcac gggtgaccgg ccagacgaag cggccccgct ccaggcgtct
     7501 ggtaaagagg cacagtccgt cactgtcagc ccacaacact tttatctggt caccccggcg
     7561 tccgcggaag atgaacagat gcccggagaa cgggtcatcc ttcaggacgt tctgaacttt
     7621 tgagaccagg ccgttaaagc catttcgcat atcggtgata cctgcaacca gccagatacg
     7681 cgaacctgca gggaaagata tcatcagtgg ctgctccctt ttatttcgcg gataagtatc
     7741 tgtaataacg ccggcgtcag tttaccttta agcctgagag ttccggccgg cagaaccagc
     7801 tcacagcaca gactgtcgga cggtgtattt atctgctctg gttcctgtgc cggagccggg
     7861 attttattat ccggctccgg cgttaacgtc acgggaagca gtgcaggcat attttttccg
     7921 gaaggcagca ggccaccttt ccggtattga tggcgccagt tgaagagcag gttatcgttg
     7981 attccgtttt cacgggcgat ctgcgccaca caggctccgg gctgcagtga ctgctccact
     8041 aaagcgattt taaattcata aggaaaatta ggcc
//
LOCUS       DAJTYH010000155         7993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTYH010000155 DAJTYH010000000
VERSION     DAJTYH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..381
                     /locus_tag="OO609_003674"
     CDS             <1..381
                     /locus_tag="OO609_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634933.1"
                     /translation="KTALENLGLGEAAKRNVGTGANQIPDMSLFASINTVTAAAQKFP
                     SGLILQCGQLNGAPNVSSTYGMRFPMTFSRVIAVVVTLNVTGAAGQPTVSATSVQNTG
                     FNITVSPGSGYGSSADAYYIAMGY"
     gene            353..787
                     /locus_tag="OO609_003675"
     CDS             353..787
                     /locus_tag="OO609_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6634934.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1560..2609)
                     /locus_tag="OO609_003676"
     CDS             complement(1560..2609)
                     /locus_tag="OO609_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6634935.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3060..4250
                     /locus_tag="OO609_003677"
     CDS             3060..4250
                     /locus_tag="OO609_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR6634936.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4640..6100)
                     /gene="gtrII"
                     /locus_tag="OO609_003678"
     CDS             complement(4640..6100)
                     /gene="gtrII"
                     /locus_tag="OO609_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR6634937.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6097..7026)
                     /locus_tag="OO609_003679"
     CDS             complement(6097..7026)
                     /locus_tag="OO609_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6634938.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7023..7385)
                     /locus_tag="OO609_003680"
     CDS             complement(7023..7385)
                     /locus_tag="OO609_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6634939.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7647..>7993)
                     /locus_tag="OO609_003681"
     CDS             complement(7647..>7993)
                     /locus_tag="OO609_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6634940.1"
                     /translation="TKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLR
                     HTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNT
                     AQEENVVYLNAR"
BASE COUNT         2461 a         1525 c         1598 g         2409 t
ORIGIN      
        1 aaaacggctc tcgaaaacct tggtttggga gaagcagcta aaaggaatgt aggtacaggg
       61 gcgaatcaga tacctgatat gagcctgttc gcgtcaatta ataccgtaac ggctgctgcg
      121 caaaaatttc cgtctggatt aattttacag tgcggtcagt tgaatggtgc cccgaatgta
      181 tcttcaacat acgggatgag gttcccgatg acgttctcaa gagtaattgc tgtcgtagtt
      241 acattgaacg ttactggcgc ggctgggcag ccgactgtat cggcgacaag tgtccagaac
      301 actggattta atattactgt gtcgcccggt tcaggatacg gttcatctgc tgatgcgtat
      361 tacattgcaa tgggatatta acaaaatgtc atatttttat tctgcatcga caaacggatt
      421 ttattcgact gaatttcacg gcaccaatat tcctgatgat gcagtggaaa tctcggaatc
      481 agagtggaaa acactgatta atgcacagag tgtaacaaaa atgattacct gtggtgagaa
      541 cggtcatcct gtcattgttg accgtccttc tccaacacca gaacaattag ccttaataaa
      601 tgatgaaaag aaatctgcac tgatagcaga ggcaacgaat gtaatagctc cgcttcagga
      661 tgcggttgat ttaggtatgg caacagatga tgaaacgaaa ctgttactgg catggaaaaa
      721 atatcgggtg ctgcttatgc gtgttaatgt agtaaaaccc gagtggccta tgcatccaaa
      781 taaatagact tgttaatcta ggataattgt tggaataata ctcgtttttc agtaggttca
      841 ggtagcctgg gtataatgct cacttctaca atgatttcaa cttattttat ttaattgata
      901 ttacgaaact taactcatac ataaacaata tgactcaata ctgtcctaaa tagctgcgca
      961 gaaaagtgct ccactttcgc tcagcccttg ctgggcgaag catcaggaag gcgttctcag
     1021 ctaggttgtg ttatatctgg cttgagtttt gttgcccata cgcaacacat tacccatctg
     1081 taaacgacaa gtgtggcaga attaggtgca gctatttaga ctatatcgac gcagtaacga
     1141 taatagatat cgaaactgta ctagatatta gctacttatg gttaccgggg ggagtctttt
     1201 gagcactggt gttatcaatt ggcttcaaat aattcgccaa taaattatat tgcgatagct
     1261 catcaccttc caggttgttt atagccagtt tatcagcgtc accacctact gtttatgctt
     1321 ttatttcttt gattaataag tatatgatat tttaattcat ctggtgtatt tctttctcat
     1381 gtgttggtcg actgaatatc tgttcagtga aggggtaatt ttatgtttgc acttcgaaca
     1441 ttcccttcag ttgctaggaa agaaaaccgt aacatttgcg cattaatttt aacagtataa
     1501 tctgagcata aatatgcgct atatatctat aaaaaggcgc aattaattgc gcctttttat
     1561 cattttatca aaaatggccc accgactgtc ctcgatagcc aatggccaat tttatctgat
     1621 acatatacac aaatacaggt atatatgatt gcgcagatag gatttggaat agtcacgctg
     1681 aagcctataa ttgctaaagc catagggata aataatttgg tgatatactc cattccacat
     1741 atgttaagag tatttcttcc agctcttaca ataaagtcat ttccacaaat tatttttgct
     1801 atgaaaatat taaagattat caagcctacc gtggtgataa tagtattaat agcttgaata
     1861 taactacttg atattatgtc tgcattgaat attgagataa tcttaaaaag taaaggtttc
     1921 tggttaagta gttgatacgc actcattata gcggtaattg agaagacaat gaaaccaatt
     1981 ttagattttc caaaaaacct gtcacgtgtt aattctaaaa acatacatct accgagaggc
     2041 aataaccacc agtatgccat agcggagtct atgttccaaa accattgagg atctagtaat
     2101 gggttatgat tgagcactgt ttgtgacaac atgaacgata acagtgatat gattaaaatc
     2161 actatattgt tcttgactat ttctctaaag attgcatcaa ttgctattat aacaaaaagg
     2221 cagtttataa accatattgt tcctacgaat tggttatttc taacaccata tattatatca
     2281 taaatgtggt tgtatatata atcaatcgtc tcacctgagt taattgtgtt tattattaat
     2341 atagaaaatg caaaagtgaa atatggaatc attaatctgt aaaactttga cttgatataa
     2401 tcgaaaacag ataagtcatt ttttttgata gtaaaaaaac cagcagcgaa aaagaatagt
     2461 gggacgtgat aactaaaaac aaaaggatac aatttgccag cccccaaccc aaggtgacct
     2521 aaatatatag caaaaatacc gagaaatttt aaagcatcaa cccaatcatg tcttattttt
     2581 ctgttgctgt cgattttgtt attatacatt taaagtaggc tctccagttt tgtgtgattt
     2641 ttaaacactc attgcaaata tgaatgcctg aaagtttatg attattaatc aacgagttca
     2701 atattaattt attatttttc aaagtcgcac tatataggca tttacttccg cagtaccacc
     2761 gatagtatcg atatcaaatc agcctgcggc cgcgataact ttcgtaacat tctgccagcg
     2821 gctttgttga ataaatcagg ttcaagataa tcgtctctgc atcaggttag agtttcaggc
     2881 aatacatcct ctttctggca tacctgccac tctcgcacca tggtcatagc ctttacgatc
     2941 tgcatactca ccagaccaga gcccgctccc ttttaggctg aggaatcccc agaaaagaag
     3001 acaggcatag agttttgtga tctactgatt gcgctaacaa attcaatgag atcacctcta
     3061 tgcctgcccg taaagtatgc cagacttttt tccgtaatgc tttagcttcc acgcatcagt
     3121 atcgacaaaa tgccattatt gattctgctg ctgctttagt cggtggcgca tctctttctc
     3181 ttaccagcat tggccggcat ttacccggtc ctgctcgcgt gaaagataaa ataaaacgcg
     3241 ttgactgtct gttgggtaat aaacgacttc ataatgatat ccctctgata ttcaaaaata
     3301 ttacatccat gatgacaaat aaactgtcgt ggtgtgttat cgctgttgac tggagcggtt
     3361 acccttttca ggaatatcat gtccttcgtg ccagcctcct ctgcgacggt cgctctatcc
     3421 ccctgatgag tcaggtcttc ccgtcaaaaa aaaataacga ggcggtggaa atcgcttttc
     3481 tggatgcgct ttccggggcc atttctcccc ggactcgtgt tgttattgtt actgatgctg
     3541 gttttcaaag tgcatggttc cgtcatatca aatcacaggg atgggatttt attggtcgta
     3601 tcaggggggt agtgaagttc aggctggata gtgacaaaga taagtggctg gacataaaaa
     3661 tgtgcagggg gagttcagag gcaaaatacc tggggacggg aaccctggcc cggaaaaaac
     3721 gctcgcagtg tgaaggacat ttttatcttt ataaacattc acctaaaggc agaaaaagca
     3781 ggcgtgccag agggaggccg ggcttaccaa caacggaaaa agaacaaaaa gccgcaggca
     3841 gagaaccatg gttaatattc agcaatacag ctgagtttaa tgccaaaaaa atcatgaaac
     3901 tgtacagtcg aagaatgcag atagagcaaa actttcggga tgaaaaaagt gaacgctttg
     3961 gttttggcct tcgggcgagc agaagtcgga ggggagagcg ttttctggta ttaagtctac
     4021 tggttacact ggccagtatc gtactctggc tacttggtta tcattttgaa aataaaggat
     4081 ttcatttaaa gtatcaggct aatagtctca aaaaacgaag agtattgtct tttctgacgc
     4141 tggctgaaaa cgtcctgaga tttaaccctg aacttctacg acgagcacaa cctgaaaaga
     4201 tattgtgccg gttggccagc acttaccgaa gtatggtgct ggcttactga tgaaaatttt
     4261 ctggggatcc ctcagccttt cggggaggaa tgagggctct gatattcttt tgccgtagtt
     4321 catcgtgaaa ccgtcggatg tcatttggcc tgagtgaccc atgcctccca aaagcagagt
     4381 cgctagtgtt atgtaattag gcggttaaac ttcaattctc agtgttaagt ttgggtaaac
     4441 gcttgtaaat ggtattgcta attttttaat tgactaatat tagaaacggt tagtgtgatt
     4501 gtatatagat atttgagtgt aaggtattta aaggggcatt cagcccctcc gtctatttgt
     4561 gattgcatcc attgatgtgt gtattatttt gagcttgaaa aagggaggcg ctctttaata
     4621 ataaataaat ctatcaaagc taaatattaa atggaagcca cccttttttc tctgatagtt
     4681 ttattatcac caaaccattt atttcagcta tcgaatctgg attcggccag gaaggtaaac
     4741 tcttcacaat tggataaatc ttctctattt gttctttatt tgctggtttg caattacaga
     4801 ttccatagta attcataaat gcaaccatcc taaaatagtt gccaccatcc caccaaaaaa
     4861 aagattttcc aaaagtgtct gcgctcatgg agagcatatt tcctgagtcg tttgaaccat
     4921 atatatatat atattttccg ttaagggaaa tgccttttgt ttgagatgtg tatgatattt
     4981 ctttagctaa agagatatct ttctgtcttg cgagagtatc attcaaaaat agatttttgg
     5041 atgttgaaac gccattaaat ataataaata aaaaaaacat gcagtttagg tattttatag
     5101 agcgaaaatt gcttaagcaa gaaaacaaaa ttactgctac tataggcatt aaaacaaaca
     5161 ggcgaggtgg cgcccctgag ccaacaacaa cgataaatat aaccggaagt atataaatga
     5221 aaatgattga tgaaatcaaa taaattgatc ttgtttttaa tttatatgtt agggtaaaca
     5281 tcagaattaa taaaggaatc agtaaccact tgtataggtt taaaccagtg ggaggattgt
     5341 tatagaagtt atatatatta tctatggctg atttaacaag ccatttaata tcgcttgcat
     5401 tttgtatata atttgagatg tactcgttcg attcaagcga aaaataatgc ttgatagctt
     5461 tggttaatag caggtaaatt aaggtagata aagctatgag ggatagagat aaacatgaac
     5521 tataaaaaga ttcacgaata tttttctcat ttcgtattat cgaatttatc tgcaacccaa
     5581 tgacgaatgc aataatatat gttacgaatg tttggtagat tgccattgaa agaatggaca
     5641 gtactatacc agaaaatatc acaatcctat ttttttgcga gtgaaaaata attgctgata
     5701 tcgctgccag tagaaaagca attcccacag tatcagcttg gttaagaaac tctaattgat
     5761 aggagatctg agggaaggta ataaatacta acatacctat caataattca taagattcta
     5821 gctttagcga tctgcagatt ataaatgctg aaatgatgat aaaagataag gctattaatg
     5881 gtgttatata aagtgaaaaa ggctcaggga gaaagtaatg tcgcaaaaaa gtgtgaaacc
     5941 accgcccaag cgtgattgtt tgataaaaat tatttgtaaa ctcgccatca atattaagtg
     6001 ttccatcgta caaccacccc caataacaat aaattgatat tgcaaaacat gatatgaagg
     6061 caaggagatt tgcattttta aataaattaa ttttaatcat gggttacctc gatgagattt
     6121 ttttatgatg tatttgggtc tattttttac ttcaatatag attctaccta tatattctcc
     6181 gagaacacca atcccgatca gttgcactcc acccaagaaa agtattgata caagcaggga
     6241 gggatacccg cgtactgggt taccaaagac aagggtgtct ataatcatcc atgcaccata
     6301 taaaaatgaa atgcttgcaa caaacaagcc tatataagtc catacgcgaa gaggaaaggt
     6361 tgaaaaactt gtgatacctt ccagtgccag attccataat ttccagccat taaattttga
     6421 gatgccagca acacgctctg cgcgtacata ttcaacgaca tccgtcggac cacccaccca
     6481 gctcagtatg cctttcatga aaagattgcg ctcaggcaac agtttaatgt tctccacaac
     6541 ctcacgagac atgagtcgaa aatctccgac attttcctcg atctttgggg tgcttatttt
     6601 gttgtgtaat ttatagaacc actcagctgt cttacgtttc agtcgtccat cagttgagcg
     6661 gtctgaacgt ttagcaagca ccatgtcagc acctgcctgc cacttttcaa taagatgagg
     6721 gataacctca attgggtctt gcaggtcgac atcaatagga attacagcat cgccgcttgc
     6781 atggtctaac cctgcaaata aggctggttc tttaccaaag ttgcgtgtaa atgacagcgg
     6841 aacaactagc gggtctgaaa cagccagcgc gttaataatt gactctgtgg catctttact
     6901 tccgtcattt atgaatacaa tttctacttc atatggcttc aactcttgga attcacgtac
     6961 cgttttatag aaaacaggta tcgcttcttc ttcattgaag acaggaacga caagagatat
     7021 tttcatttcg catccctaaa gacaatgaac tttgaataga cgaaaccgca caccagactg
     7081 atggcggaga aggtgataag agtgactatt ggaggaagtg agcatttatc agctgcccaa
     7141 ccaacaatca cactcaatat tcccataaat cccacgtata acatgtagcg catcgctgta
     7201 gtcaatgctt tgaatgtgaa tcttgcattc gcgaagaagc taaagctcac agccactacg
     7261 aaacctgtga agtttgccag agcctgactg gtatgtgcgg catagataca tacaccaaaa
     7321 accacccagt gtataagtgt gttcagcaca cctatagatg tgtactttac aaataacttt
     7381 aacatttatt taatcaatga gctctgaaag gcatgaagtc tatcatccaa gtctcaattg
     7441 atcgatactt gctatgtctg atgagacaaa actgagacac ataaggcctc acaatggctt
     7501 gcaaggcttt acatgttttg atgtggtggg acgtgtgagc gcagtgttga tgggataatc
     7561 ctttgaatta caagcggatt cttataattc gtaatgcgaa ggtcgtaggt tcgactccta
     7621 ttatcggcac catttaaatc aataagttac ctcgcattta agtaaactac gttctcctct
     7681 tgtgccgtat ttgtgccatt gcgacttata atcgcatcga ttttgctcgc gtgctcggta
     7741 agatgcccgg ctgaaaggtg ggcgtatctt tgaaccattt cgagagtttc ccatcctccc
     7801 atctctttaa gcgcaagaag agagacaccg gactgaacca gccagcttgc ccaggtatgc
     7861 ctcaggtcat ggaagcggaa gttgctaatg cctgcccgct ttaacgctcc cttccatgcc
     7921 ttgttgctgt cggttctcat cttccttacc gctgctgttt ttgttccgtc gcttcggtag
     7981 gcaggtttgg tgt
//
LOCUS       DAJTYH010000156         7915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTYH010000156 DAJTYH010000000
VERSION     DAJTYH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1659
                     /locus_tag="OO609_003682"
     CDS             <1..1659
                     /locus_tag="OO609_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6634941.1"
                     /translation="MVLGVIGYFIEHKSRNTLLFQPTDSAAEDFMKSHVEATIRDVPC
                     LKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSVDVVCYDELSSFEPD
                     VEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGSCQIEKAANESAHFMRFYVPCPHCG
                     EEQYLKFGDDATSFGLKWEKGKPETVYYLCEHNGCVIRQSELDQTGGRWICDNTGMWT
                     CDGLTFFSASGNEIPPPRSITFHIWTAYSPFTTRVQIVCDWLDALKDPNGVKTFVNTT
                     LGETWEEAVGEKLDHQVLMDKVVPYTATVPVRVVYLTAGIDSQRNRFEMYVWGWAPGE
                     EAFLVDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDIGGIDGEIVYQ
                     RSKKHGIFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADPT
                     PVNEATSYAIRFPDNPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRRNEALDCL
                     VYAYAALRVSVQRWQLDLAVQLHPARMQLHNRPLKNWQRSCPEE"
     gene            1660..1863
                     /locus_tag="OO609_003683"
     CDS             1660..1863
                     /locus_tag="OO609_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6634942.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            1860..3453
                     /locus_tag="OO609_003684"
                     /pseudo
     CDS             1860..3453
                     /locus_tag="OO609_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3443..4948
                     /locus_tag="OO609_003685"
     CDS             3443..4948
                     /locus_tag="OO609_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6634943.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            4985..5332
                     /locus_tag="OO609_003686"
     CDS             4985..5332
                     /locus_tag="OO609_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6634944.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5390..6418
                     /locus_tag="OO609_003687"
     CDS             5390..6418
                     /locus_tag="OO609_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6634945.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6470..6856
                     /locus_tag="OO609_003688"
     CDS             6470..6856
                     /locus_tag="OO609_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6634946.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            6868..7245
                     /locus_tag="OO609_003689"
     CDS             6868..7245
                     /locus_tag="OO609_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6634947.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7232..7816
                     /locus_tag="OO609_003690"
     CDS             7232..7816
                     /locus_tag="OO609_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6634948.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            7813..>7915
                     /locus_tag="OO609_003691"
     CDS             7813..>7915
                     /locus_tag="OO609_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6634949.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFID"
BASE COUNT         1815 a         1876 c         2470 g         1754 t
ORIGIN      
        1 atggtgcttg gtgtgatcgg gtattttatt gagcacaaat cccgcaacac tcttcttttt
       61 cagccgacgg actcagctgc tgaagatttt atgaagtctc acgtggaagc aacaattcgt
      121 gatgttcctt gcctgaaaaa actttctccc tggctgggcc ggaaacaccg ggataatacg
      181 ctcacactga agcgtttttc ctctggtgtg gggttctggt gtttgggtgg tgctgctgcc
      241 aaaaactacc gtgaaaaatc tgtcgatgtg gtctgctatg acgaactctc ctcgtttgaa
      301 ccggatgtgg aaaaagaagg ttcgccaaca ctgcttggcg ataaacgtat tgaaggttcg
      361 gtatggccaa aatccatacg cggttcaacg ccaaaaatta aaggctcctg tcagattgaa
      421 aaagctgcga atgaatctgc gcatttcatg cggttttatg tcccttgccc tcattgcggg
      481 gaggagcagt atctgaagtt tggcgatgat gcgacgtcgt ttggcctgaa atgggagaag
      541 ggtaaaccgg aaacggtgta ttacctgtgt gaacataatg gctgcgtgat ccgtcagtct
      601 gaacttgatc agaccggggg gcgctggatt tgtgacaata ccgggatgtg gacgtgtgac
      661 ggtctgacgt ttttcagtgc ttcgggtaat gaaattccgc cgccgcgctc catcactttc
      721 catatatgga cggcgtacag tccgttcacc accagggtac agatagtctg tgactggctg
      781 gatgcactga aagatcccaa cggcgtgaaa acttttgtga acaccacgct gggcgagacc
      841 tgggaagagg ccgtgggcga aaaactcgat caccaggtac tgatggataa ggttgtgcct
      901 tacacggcaa cggtgcctgt ccgggtggtt tatctgacgg cgggcattga ctcgcagcga
      961 aaccgttttg agatgtatgt ctgggggtgg gctccgggag aggaagcctt tctggtggat
     1021 aaaatcatca tcatgggacg tcctgatgag gaagagacgc tgttacgtgt ggatgcggcg
     1081 atcaataaaa aataccgcca tgcagacggt accgaaatga ccatttcccg tgtctgctgg
     1141 gatatcgggg ggatcgatgg cgaaatcgtg tatcagaggt caaaaaaaca cggtattttc
     1201 cgggtactgc cggtaaaagg tgcctctgtc tatggcaagc cggtgatcac catgccgaaa
     1261 acccgcaatc agcggggtgt gtatctgtgt gaggtgggga cggataccgc aaaagaaatt
     1321 ctctatgccc gaatgaaggc cgatcccacc cctgtgaatg aggccacatc gtatgccatc
     1381 cgctttcctg ataatccgga gattttttcg cagacagagg cacaacaact ggtggcggag
     1441 gaactggtgg aaaagtggga aaaaggaaag atgcgtctgt tgtgggataa caaaaagcgg
     1501 cgtaacgaag cgctggactg cctggtgtat gcctatgcgg cattacgtgt gtccgtgcaa
     1561 cgctggcaac ttgatctggc tgtgcagttg catcccgcaa ggatgcaact gcacaaccga
     1621 cccttgaaga actggcagcg aagctgtccg gaggagtgaa tggttacagt cgctgaactg
     1681 caggcgctgc gtcaggcgcg ccttgattta ttaaccggta aacgggttgt gtcggtacag
     1741 aaagacggtc gcaggatcga atatacggca gcgtccctga atgagcttaa ccgtgcgatc
     1801 aacgatgcgg agtcggttct gggtacgacc cgacgccgcc gaccgctggg agtgaggtta
     1861 tgaaacgaac gcctgtcctg attgatgtga acggcgttcc gcttcgtgag agtctcagct
     1921 acaacggagg cggcgcagga tttggcgggc aaatggcaga gtggttacca ccggcgcaga
     1981 gtgccgatgc ggccctgttg cctgcattgc gtctggggaa tgcccgggca gatgatctgg
     2041 tgcgcaataa cggtatcgcg gctaatgcgg tggccctgca taaggatcat atcgtcgggc
     2101 acatgtttct tatcagctac cgtctgaact ggcgttggct ggggatgcgg gagaccacgg
     2161 caaaaagttt tgtcggggag gtggaagcag cctggtcgga atacgcagaa ggaatgtttg
     2221 gcgagatcga cgtggaaggg aaacgcacgt ttacggaatt tatccgtgaa ggggtgggcg
     2281 tgcatgcctt taacggcgaa atctttgtgc agccggtctg ggatacggaa accacgcagt
     2341 tattccgtac gcgttttaaa gccgtgagtc cgaaacgggt ggacacgcca ggacacggta
     2401 tggggaacca ctttctgcgg gccggggttg aggtcgatcg gtatgggcgg gcgattgcgt
     2461 accatatctg tgaggatgat tttccgttct ctggtgccgg acgctgggag cggatcccgc
     2521 gtgagcttcc caccgggcgt ccggccatgc tgcatatttt tgagccggtg gaggacgggc
     2581 agacccgtgg ggctaatcag ttttacagcg taatggagcg gatgaagatg ctggactccc
     2641 tgcaggccac acagcttcag tcggccattg tgaaggcgat gtatgcagcc acaatcgaaa
     2701 gtgaacttga taccgaaaag gcatttgaat atatcgcggg ggcaccgcaa ggtcagcagg
     2761 ataatccgct tattaatatt ctggagaagt tcacccgctg gtatgacacg aataacgtga
     2821 cgctgggcgg tgttaaaatt ccgcaccttt tttcctggcg atgcgctgaa cctgcagaca
     2881 gcgcaggatt cagacaatgg attttcggcg cttgaacagg cgctgctgcg gtatattgca
     2941 gccggtcttg gcgtttccta cgaacagttg tcccgtgatt actcgaaggt cagttactca
     3001 agtgcccgcg cctccgccaa tgagtcgtgg cgctatttta tggggcggcg aaaatttatt
     3061 gcggcccggc tggccacgca gatgttttcc tgctggctgg aagaggcgct tcttcggggg
     3121 attattcgtc cgccacgggc gcgttttgat ttttaccagg cgcgttcagc ctggtcacgg
     3181 gcagagtgga ttagctccgg aagaatggcc attgacgggc tcaaggaggt ccaggaatcg
     3241 gtgatgcgca ttgaggccgg actgagcacg tatgagaaag aactggcact gatgggcgaa
     3301 gattatcagg acattttccg ccagcaggtc agggaatccg cagagcgcga aaaagccgga
     3361 ctctcacgtc cggtgtggat agcccaggcg tatcagcagc agatagcgga gagtcgcagg
     3421 ccggaagagg agacaacatc acgtgagacg taatctttca catattatcg ctgcggcatt
     3481 caatgagccg ctgcttctgg agcccgccta tgcgcgggtt ttcttttgcg cgctcgggcg
     3541 tgagatgggc gcagcaagcc tttcggtacc gcaacagcag gtacagcttg acgctcccgg
     3601 gatgctggct gaaacggacg agtacatggc cggaggcaaa cgacctgccc gtgtttaccg
     3661 ggtggtgaac ggtattgctg tactgccggt gaccggcacg ctggtacacc ggctgggtgg
     3721 catgcggcca ttttccggaa tgactggcta tgacggcatt gtcgcctgtc ttcagcaggc
     3781 aatggcggac actcaggtac ggggcgtact gctggacatt gacagttctg gcgggcaggc
     3841 caccggcgcg tttgactgcg ctgacatgat ttaccgtctc cggcagctga agcctgtctg
     3901 ggcactgtgc aatgacacgg cctgttcagc cgccatgtta ctggcatcgg cctgctcccg
     3961 acggctggtt acccagacat cccgtatcgg ctccattggg gtgatgatgg gccatgtcag
     4021 ctatgccggt catctggcac aggccggtgt ggatatcacg ctgatttacg ccggatcgca
     4081 caaggtggat ggcaatcagt ttgaatccct gccagcaaag gttcgccagg acatgcagca
     4141 gcgcattgat gcggcgcgcc ggatgtttgc tgaaaaagtg gcgatgtata ccggtttatc
     4201 tgtggatgcg gttatgggaa cagaggccgc cgtttttgaa ggtcagtccg gtattgaggc
     4261 cgaactggcg gatgaattaa tcaatgcgtc ggacgccatc agcgtgatgg ctgcgacgct
     4321 gaacacacac gatacaggag gcactatgcc gcaattaact gcaacggagg ctgccgcgca
     4381 ggagaaccag cgagtgatgg ggatcctgac gtgtcaggaa gcgaaaggac gtgaacaact
     4441 ggccacgatg ctggcagggc aacagggtat gagcgttgag caggcccggg cgattctggc
     4501 cgtggcggca ccgcaacaat ctggtgtatc tgcgcagagt gaagccgatc gcattatggc
     4561 atgtgaagag gcgaaaggac gtgaacaact ggcggcaacg ctggcggcga tgccggagat
     4621 gacggtggaa aaagcccgtc cgattctggc tgcttcaccg caggcgaatg ccggaccctc
     4681 acttcgtgat cagattatgg ccctggatga ggcaaaaggg gcagaggcgc aggctgaaaa
     4741 actggcggcc tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg cggcagcctc
     4801 aggcagggca gaaccggttt ctgcatccac aaccgccatg tttgaacatt tcatggcgaa
     4861 ccattcaccg gcagcggtac agggtggcgt gccacagacg tcagcagacg gtgatgcgga
     4921 cgtgaaaatg ctcatggcca tgccatgaag tcagtgctga ccatcaatat gaggttttta
     4981 caatatggta acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt ttgccggtaa
     5041 tgatccggcc cataccgcca caggctgcag cgggatttcc tcggcaacac cggctctgac
     5101 gcccctgatg ctggatgagg ataccgggaa actggtggtc tgggacggac agaaggcagg
     5161 ccatgtggtt ggcatactgg tactgccgct tgaaggtaca gagacggtgc tgacgtatta
     5221 caagtcgggg acctttgcga cggaggccat ccactggcct gagagagtgg atacacacaa
     5281 aaaggcgaat gcctttgccg gcagtgccct gagtcacgca gcgctgccgt aacattttat
     5341 caggtcactg cggtagcctg actgatttct gaatgaaagg aactgattta tgggattgtt
     5401 tacgacccgc cagttactcg gttataccga acaaaaagtt aaatttcgtg cgctgtttct
     5461 ggagctgttt ttccgccgta cggtgaattt ccataccgaa gaggtaatgc tggacaaaat
     5521 taccggaaaa acgccggtgg cggcctatgt ctccccggtt gttgaaggaa aggtgttgcg
     5581 tcaccgtggc ggtgaaactc gtgtactgcg cccgggctac gttaagccga aacacgaatt
     5641 taattaccag caggcggtgg agcgtcttcc cggtgaagat ccggcccagc tgaacggccc
     5701 ggcttaccgt cgcctgcgta tcatcaccga taacctcaaa caggaagagc acgccattgt
     5761 ccaggtggaa gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca ccatggaagg
     5821 agagcagttt gaaactgttg aggtcgattt tggccggtcg gtgggaaata acattacgca
     5881 ggcaggaggg acggagtgga gcgctcagga ccgcgatacc tttgatccga cgcatgatct
     5941 ggatgcgtac tgtgattttg cttccggcac catcaatatt gccattatgg atggtaaggt
     6001 ctggcgtctg ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc gtcgtggctc
     6061 aaattcacag ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct tcaaagggta
     6121 ttatggtgat ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg acggtaccga
     6181 aaaacgttat ctgccggacg gcacactggt actgggaaat acggccgcag agggtattcg
     6241 ttgctacggt gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg cgtcttcccg
     6301 ttatccgaaa cactggctga ccgtgggtga tccggcgcgt gaattcacca tgacgcagtc
     6361 cgcgccgctg atggtgctgc cggatccgga tgagtttgtg gtggtgcagg tgaaataatc
     6421 cgtgagcggg agctaaatgc tcccgttttt cattcaatca ggagcgggta tggctacgaa
     6481 agaagaaaac ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg aaccggatgt
     6541 ctccggtagt gcggcagata ttgcgcagcg agtggcggaa ctggaggaag agcttggaga
     6601 tgcgggggag gtcgcagagc cggatacgta ctcaccatcg aaagatgatc cgaccgcgca
     6661 taaaggcgag tcagttcctg aacagccagg cattgttacc ggagacgaaa cggggcaggt
     6721 tacggtggtg gcactggcga cactgcatac aaaatgtaat ggttcagtga tatttgtttc
     6781 accgggaacg tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg caggtggtct
     6841 ggcaaaaagg cagtaacagg agatgtcgtg gacggtttcg ataatctgtt tgatgctgcg
     6901 cttgccggtg tcaatgaagt cattctccgt gatatgggga tcagcgccgt cattacgtcc
     6961 ggagaacttg aggggacaca cctgacaggg gtttttgatg atcccgaaag tatttctttt
     7021 gttgccgggg ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa aacggcagat
     7081 atatcgcagt tacgccgtca ggatacgtta actatcgggg atgattcttt ctttgtggat
     7141 cgtattactc cggatgacgg gggatgctgt tatatccggt taaggcgggg cagtcctgcc
     7201 ccacagaata atgccagaat gaggcgatat gatgaaggga cttgagaatg ccatccgcaa
     7261 tctgaacagc ctcgataccc gcatggtgcc acagaccagc gcatgggcgg taaaccgtgt
     7321 ggcggcaaag attgtttctg tcgccacacg gcaggttgcg cagaataccg ttgccgggga
     7381 taaccaggtg aaaggtattc ccctgaaaac ggtcagggag cgtgtcaggc tgcttaaagc
     7441 cagcccctca ggaaaaatgt atgcccgcat gcgtgttaac cggggtaacc tgcccgccat
     7501 taagctgggt accgcacagg tcagactggc ccgctcccgg catggcagca actcacgtca
     7561 tcgtggcagc gtactgaaag tggggaaata ccttttccgg gatgcgttta ttcagcaact
     7621 ggcgaatggt cgctggcatg tgatgcggcg tattgacggc aaaaatcgtt accccattga
     7681 tgtggtgaaa atcccgatgt ccggaccgct gacacaggca tttgaagatg cccgcgaccg
     7741 catcattgct gcggaaatgc cgaaacagct ggggtatgcc ctgaaacaac aactgaggtt
     7801 acatctgagt aaatgaaccg acacacacaa atccgtcaga gcgtgctggc acgccttcgg
     7861 gaacagtgtg gagacagcgc cacgtttttt gacgggcttc cggcatttat tgatg
//
LOCUS       DAJTYH010000157         7909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTYH010000157 DAJTYH010000000
VERSION     DAJTYH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..775
                     /gene="espL1"
                     /locus_tag="OO609_003692"
                     /pseudo
     CDS             1..775
                     /gene="espL1"
                     /locus_tag="OO609_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            947..1054
                     /locus_tag="OO609_003693"
     CDS             947..1054
                     /locus_tag="OO609_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634950.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1107..1865)
                     /locus_tag="OO609_003694"
     CDS             complement(1107..1865)
                     /locus_tag="OO609_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6634951.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2152..3081
                     /gene="pfkB"
                     /locus_tag="OO609_003695"
     CDS             2152..3081
                     /gene="pfkB"
                     /locus_tag="OO609_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6634952.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3182..3472
                     /gene="ghoS"
                     /locus_tag="OO609_003696"
     CDS             3182..3472
                     /gene="ghoS"
                     /locus_tag="OO609_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6634953.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3578..4438
                     /locus_tag="OO609_003697"
     CDS             3578..4438
                     /locus_tag="OO609_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6634954.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4479..5015)
                     /locus_tag="OO609_003698"
     CDS             complement(4479..5015)
                     /locus_tag="OO609_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6634955.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5162..5830
                     /gene="hxpB"
                     /locus_tag="OO609_003699"
     CDS             5162..5830
                     /gene="hxpB"
                     /locus_tag="OO609_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6634956.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5993..6583
                     /locus_tag="OO609_003700"
     CDS             5993..6583
                     /locus_tag="OO609_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6634957.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6716..7909
                     /gene="tcyP"
                     /locus_tag="OO609_003701"
                     /pseudo
     CDS             6716..7909
                     /gene="tcyP"
                     /locus_tag="OO609_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2069 a         1886 c         1891 g         2063 t
ORIGIN      
        1 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
       61 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      121 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      181 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      241 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattagccac tccaatagca
      301 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      361 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      421 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      481 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      541 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      601 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      661 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      721 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      781 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
      841 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
      901 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
      961 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1021 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1081 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1141 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1201 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1261 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1321 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1381 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1441 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1501 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1561 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1621 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1681 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1741 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1801 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     1861 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     1921 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     1981 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2041 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2101 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2161 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2221 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2281 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2341 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2401 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2461 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2521 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2581 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2641 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2701 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2761 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     2821 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     2881 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     2941 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3001 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3061 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3121 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3181 tatggccagt ggcgatcttg tccattatgt cataaccgtc atgttgcatg aggatacatt
     3241 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3301 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3361 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3421 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3481 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3541 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3601 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3661 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3721 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3781 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     3841 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     3901 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     3961 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4021 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4081 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4141 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4201 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4261 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4321 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4381 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4441 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4501 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4561 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4621 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4681 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4741 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4801 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     4861 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     4921 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     4981 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5041 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5101 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5161 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5221 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5281 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5341 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5401 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5461 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5521 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5581 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5641 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5701 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5761 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     5821 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     5881 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     5941 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6001 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6061 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6121 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6181 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6241 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6301 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6361 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6421 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6481 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6541 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6601 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6661 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6721 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6781 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     6841 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     6901 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     6961 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7021 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7081 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7141 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7201 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7261 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7321 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7381 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7441 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7501 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7561 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7621 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7681 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7741 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7801 ggcattaacc cgctgggccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     7861 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactga
//
LOCUS       DAJTYH010000158         7714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTYH010000158 DAJTYH010000000
VERSION     DAJTYH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..1264
                     /gene="yjeM"
                     /locus_tag="OO609_003702"
                     /pseudo
     CDS             1..1264
                     /gene="yjeM"
                     /locus_tag="OO609_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1415..4726)
                     /gene="mscM"
                     /locus_tag="OO609_003703"
     CDS             complement(1415..4726)
                     /gene="mscM"
                     /locus_tag="OO609_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6634958.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4748..5716)
                     /gene="psd"
                     /locus_tag="OO609_003704"
     CDS             complement(4748..5716)
                     /gene="psd"
                     /locus_tag="OO609_003704"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6634959.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5813..6865)
                     /gene="rsgA"
                     /locus_tag="OO609_003705"
     CDS             complement(5813..6865)
                     /gene="rsgA"
                     /locus_tag="OO609_003705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6634960.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6960..7505
                     /gene="orn"
                     /locus_tag="OO609_003706"
     CDS             6960..7505
                     /gene="orn"
                     /locus_tag="OO609_003706"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6634961.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1741 a         2009 c         2085 g         1879 t
ORIGIN      
        1 atagtgtcgg accacgtttt gccttcattg gtacgtttat gtggttttcc tcttatatca
       61 tctggatggt gagtacctcc gcgaaagttt gggtaccgtt ctcaacattc ctctatggta
      121 gtgacatgac ccagcactgg cgtattgccg gactggagcc tacgcaggtg gttggtctgc
      181 tggcagtagc atggatgatt ctggtcacag tcgttgcttc aaaggggatt aataaaattg
      241 cccgcattac tgcggtgggc ggtattgcag taatgtgtct gaatttagta ttgctgttag
      301 taagcattac tattttgtta ttaaatggtg ggcatttcgc gcaggatatt aatttccttg
      361 catcaccgaa cccgggttat cagtccggtc tggcaatgct atcgtttgtg gtatttgcta
      421 tttttgccta tggcggaatt gaagcggttg gtggtctggt cgataaaacg gaaaatccag
      481 aaaagaactt tgccaaaggt attgtttttg ccgctattgt tatttcaatc ggttattcgc
      541 tggcaatatt tttatggggc gtcagcacaa actggcagca ggtattaagt aatggttccg
      601 ttaaccttgg caatattacc tatgtgctga tgaagagcct cggggtgatg ctgggtaacg
      661 cactgcattt gtcacctgaa gcgtcattgt cgctgggcgt atggtttgcg cgtattaccg
      721 gactttcgat gttcctcgct tataccggtg cgtttgctat tcaccgctga aagccatcat
      781 ccaggggacg ccgaaagcgt tgtggccgga accgatgacg cgcctgaatg cgatggggat
      841 gccgtctatc gccatgtgga tgcagtgcgg gttggttact gtcttcattc tgctggtttc
      901 gtttggtggc ggtaccgcat cggcgttctt taacaagctg acgctgatgg cgaacgtgtc
      961 tatgacgctt ccttacctgt tcctcgcgct ggctttcccg ttttttaaag cacgtcagga
     1021 tctcgacaga ccatttgtga ttttcaaaac gcgtatgtcg gcaatgattg cgacggtggt
     1081 tgtcgttctg gtggtgacat ttgcgaacgt cttcaccatt attcagcctg tggttgaagc
     1141 cggagactgg gacagcacat tgtggatgat tggcggccct gtcttcttct cgctgttagc
     1201 gatggcgatt taccagaact attgcagtcg catggcgaat aaacctgagt tagctctcga
     1261 ctgatcatat gaaatcctgc aactcatcgt ctctccggcg atgagttgtc aggaagtaat
     1321 gcgtatggat gatactctcc agggccggtg ataactgaag atgatgttaa gctgctaaat
     1381 gagccacgtt gacatcattt ggcagcttca tgcattacaa acttcccgcc tgacgacctt
     1441 tgcccgcaga cgtcagcgtt ctccccgttt gtttaccgtt gaggctttcc agacgcatct
     1501 ggaagggcgg gaatggcata tcgataccgt gggcgtggaa gcctgccaga atcagctggt
     1561 ggatctcatg gcgtagcggc atacggtgac ccatctcagc ggcgtaaata cgcagctcga
     1621 aaatctgaat cccctgttgc agatccacca ggaagacttc cggtgccggg ttgtcgatca
     1681 ccagcgagca gcgacgcgct gcggtgagca ggatttccgt cacttcttca ctgttggcat
     1741 cggcaggggc cggaatcgtc aacactacgc gcgtgaccga gtcagagagc gaccagttga
     1801 taaactgctc ggtaataaac gccttgttcg gcacgattat ctctttacgg tcccagtcgc
     1861 tgatggtggt ggcgcgggtg ttaatttttg tcacgctacc agtaagatcg cggatcgtta
     1921 ccgtatcgcc aatgcgaatc ggtttttcga acaggatgat caggccagag ataaagttgg
     1981 cgaaaatttc ctgcaaacca aaaccgagac caacaccgag cgcggcaacc agccactgca
     2041 atttcgacca ctcaatacca atcattgaga agccgaccag cccgccaatc agcatcagca
     2101 gatattttgt gatggtggtg atggcgtaac ccgtacccgg cgttaaatcc aggtgctgca
     2161 aaatcgccag ttccagcagc gcgggcaggt tgcgcaccag ctgcgtggtg atgataaata
     2221 ccagaatggc aatcagcacc gcaccgaggg taattggctc cagactttct acgccctgta
     2281 ccgtggaggt gacatcccac agcgaaatat tttcgaggaa gccgaaagcg gaatggattt
     2341 ctgaccacag cacaatgacc gaaagcaggg cgatcaacat caaaattgag cgcaccagcc
     2401 gcaaggattg cgcactgatg gcatcgagat cgacttcgct ttcatcgact tcaattgctc
     2461 cttccgggct actgtgatga tgcgcttcct cttcgccacg cgcacgttgc gctaacatct
     2521 ctgcccggcg atgcttcgcc cggtcaaacg ccagcctgcg acgctggatc agcatccagc
     2581 ggcggataac gtgataaacc accagtagca ggaaccagat ggcaaccgag gtttcaagcc
     2641 tcgctaacag tgcctgcgcc gttgccagat aacccaccgc cgacgccaga atggcaacca
     2701 gtggtgcgcc aatcatcatg ttccacagca tatggttggt aatgttgtcg ccgctgcctt
     2761 ctttgttgag atacaacgga ataccggctt ttttcaggct tagggtgacc accgccagcg
     2821 caccgcaaat gaggataaag caaagccgtc ccagcgaacc ggaaaattcg cggtcgtcga
     2881 ggttatcgaa catcatcagc gccataatca gcccgatgct catcaggtag tagcgcatcc
     2941 cacgggaaac acgttcgcgc ggccagccaa aatgagcgat aaacaagccg ttcgggcggg
     3001 caaaggtggc gcaaatcatc actacccaca gcagcggcac ggtggccgtt acaccatcgc
     3061 caatcgcgac cgccagcgga taaggccacg cctcgcgcaa gccgtaaccc agcgtcatcc
     3121 acagcaccgg taacggtgac gcgacgagaa tcgaccagaa aagagtgcgc aacgtcagcc
     3181 agaagtgatc ctgagtcact ttgccgactt tcgccgccga acgttcaaga aaacgggtga
     3241 aatagcggcg cgagtaaata ctgcaaccga ccagaatcaa cgcgccaaac agcggcaaaa
     3301 ttgtctcttt gctggtcagc atcatcacac tggctttgcc caactgactg aaggtgtcca
     3361 gcgaaatgag acgacgcaga tcctgggcga tttccagcgg ccaggcgatg gtcatcgggc
     3421 gcacgtcaga ggcccagaac agatagcggt gcgttgcttc gttcacctct ttcagcgcat
     3481 cctccagttg cctgttggag actttcagct tggtcagttc caatagtagc gtgtcgccac
     3541 cctgcaacaa tgagttcagc aactcacgct gagtgcgcag ttgtgcttcc agaatacggt
     3601 tttgctcggc ggtcagcggc tgaccgtcgg cctgatggat ttgccgtagc agcggctgtt
     3661 tattaagtaa atcctcataa cgtaaccgtt gcacacgcaa ctgcgccatt tcggtatcaa
     3721 gctgttgtgg ttttggcatt tctggtagcc gtgccacctg cgcccgcagc gcttcgccga
     3781 gcaggttgga cgatcccagc cattgcgact gttcacgcag cgtattcaac gcctgccgga
     3841 cctgtaacgt ctggctggca gcctgacgct gttgcgaggc aacgagctcc atccgctgcg
     3901 cctgttgatt cagcgctgcc gatagttcgc ggttaatttt gaattgcgcg acgatatctt
     3961 tcggcaaatc ggcactgttt tctgccagta attcggtact ttccagcgcc cgctccgcct
     4021 caagctgacg ttggctattc aactgattac gtaaggcctg caaatacgca tccagttgct
     4081 ggctctcttt ttccgccagc tctgagcgta agcgcgctaa ttcctggcgg ttattggcag
     4141 acagctgcgc cagctccagt tcatcaacga gcgccttaag acgtgcagag tcagactgca
     4201 acgcgaaatt ttgtgcctga ttgagcggag tattgccggt aagcgttccc aggcggcgct
     4261 cgatctcatt taactgacgg cgggcgtcgg tttgctgttg cggcagttga ttcagcgaat
     4321 cggcaatctc gcgggcgcgc tcctgctctt gctgggcctg acggctttta tccagcaact
     4381 ggctgctgac ctggagaatt tcctgattca gcgcgtcggt agacattccc ggcgacacgc
     4441 tgcgcggctc gtcacgaatg ttgtttaatt gtgcgcgcag agtagcggag agtttcggat
     4501 aattatcgat aacttgctga tattgtttga tgcgctcaag ggaacctttt cgttcctcaa
     4561 gcgcatttaa ggcagactgg agcgcctcta cgacttccgg ctgtgcgggt ttcgccgctt
     4621 ttgcctgctc cagttcctga gtgatttgtt tgctatcggg ggccgtcgcg gcgtacgccc
     4681 cccaactgag gcaccaggcc atcagaaaag tgataatcag gcgcacgtca gcgtttcctt
     4741 tgatggatta gacctggtct tttttatcgt caaccaatgg gctggcgtcg tgttctgctt
     4801 cgatctcttc agcaggaagt ggggcgggtt cagcgtctgg cgtaacaaag gtttcggtag
     4861 atactgccag cggctggcca attttcgtga cagacaggct ttccagttgc tcaaccagat
     4921 tcactttacc cggtgcaaac aggttgataa cggtggaacc gagtttaaag cgacccattt
     4981 cctggccttt cagcagtgcc acagaaccgt cgttttcccc ggcaggccag gtccagcgct
     5041 tgatgatacc ttcgcgcggc ggcgtaatgg tgcccgccca gaccgtctca atgctgccaa
     5101 caatcgtcgc tccgaccaga atctgcgcca ttgggccaaa ttcggtatcg aaaaggcaaa
     5161 tcacgcgttc gttacgggca aacagatttg gcacgttctg agcggtgaga tggttaacgg
     5221 aaaagagatc gcccggcacg tagatcatct cacgcagaat accgttgcac ggcatgtgta
     5281 cgcgatggta gtcacgcggg gagaggtaag tggtcacaaa cgtaccgttg cggaacaggt
     5341 ccgccatcag atagttgcct gccagcaggg cttcgaggct gtagttgtgg cctttggctt
     5401 gcaggatttt atcttcttcg attttaccca actggctgat aacgccatcg gcaggcatga
     5461 ccagtacatt cggatcggta tcgattgggc gtacttcgtc acgcagcgga cggacaaaga
     5521 attcgttaaa ggtgcggtag ctggcggtgt ccggcttttg cgcctctttc atgtcgacct
     5581 tgtagtattt aacgaacaga tcgataacca gttttgtcag ccatcctgcc cgcttgcttg
     5641 cgccccaacc cgccaggcga gtaagccata gtttcggcag aatgtactgt agcgaaagtt
     5701 taaatgaatt taacaaggta gcctccaggc cattgttttg tcgttcctga tccggccaac
     5761 atgccggatc ctgaaaaaaa ggggacgatt ctaacgacgg ttagcttaat tgtcagtcat
     5821 ccgtatcaga aaagttttta cgcgttttta cctgcgccat gctttccaga atacggtgat
     5881 agttttcgaa acgggtttcc gcgattttcc cttcctcaac cgcttcccgg atagcgcagc
     5941 ccggatcggt atcgtgtttg caatcgcgat atttacacag acctaaatag tcatggaatt
     6001 cgacaaagcc ctgagtgatt tgttccggct ccaggtgcca gaggccgaac tcacgcacac
     6061 caggggaatc aatcacatca ccgccgtgcg ggaagtgata cagccgagcg gcggtcgtgg
     6121 tgtgctgacc gagccccgag ttgtcagaga catcgttggt caggatctct ttttgcagcc
     6181 ccagcaacgc gttcagcagg ctggatttac cgacgccaga ctgcccggca aaaatgctga
     6241 tgcgcccggt caacgcctct tccagcggtt tcagcccatc ctgagtatgg ctggaaacca
     6301 tcaatacgcg ataaccgatg ttgcggtaga tatccatctg ctcgttgacg aacgccatgc
     6361 cttcgtcgtc cagcaggtct atcttgttga gcacgataat cggctcaatc tgcaaggttt
     6421 cgcaggccac caggtaacgg tcgataatat tgagcgacag ctccggcaaa atggcggaga
     6481 caatgacaat ctggtcgatg ttggcggcta tgggtttcac gccgtcgtag aaatccggac
     6541 gcgttaacac cgaggtgcgc tcatgcaccg cttccacgat ccctttgaca tttacgcctt
     6601 ccgccgccgg tttacccgga cgccagacta cgcggtcgcc ggttaccagc gaacggatgg
     6661 tacggcgaat attgcagcgg tgaacgtcgc catcggcgga ttccacatca gcgtgcatac
     6721 caaagcggct gatgacgata ccttcatcag gctcgccaaa cagattgtcg tcgtagtcgg
     6781 gcttctcctt agacgtttta agacgacgct ggtgattggc gttcacgcgg cgctgctggc
     6841 ctttggagag tttattttta ctcaatcgta ctgactcctg gtcgcccctg atgggcaaaa
     6901 catctatgat acacgcaatt gtggatcaat atagtcactg tgaatgggtg gaaaatagca
     6961 tgagtgccaa tgaaaacaac ctgatttgga tcgatcttga gatgaccggt ctggatcccg
     7021 agcgcgatcg cattattgag attgccacgc tggtgaccga tgccaatctg aatattctgg
     7081 cagaagggcc gaccattgca gtacatcagt ccgatgaaca gctggcgctg atggatgact
     7141 ggaacgtgcg cacccatacc gccagcgggc tggtagagcg cgtgaaagcg agcacgatgg
     7201 gcgatcggga agctgaactg gcaacgctcg aattcttaaa acagtgggtg cctgcgggaa
     7261 aatcgccgat ttgcggtaac agcatcggac aggaccgtcg tttcctgttt aaatacatgc
     7321 cggagctgga agtctacttc cactaccgtt atctcgatgt cagcaccctg aaagagctgg
     7381 cgcgccgctg gaagccggaa attctggatg gttttaccaa gcaggggacg catcaggcga
     7441 tggatgatat ccgtgaatcg gtggcggagc tggcttacta ccgcgagcat tttatcaagc
     7501 tgtaaaattt ttcctggtca cgtaagcgcc ggaatccact tgcggcggcg tgattttgac
     7561 gctaaatcga acgtcttgct gataatttca gcacttgaga taaaaacgca aaaaaaactt
     7621 ttttgggggg ttgcagaggg aaagatttct cgtataatgc gcctcccgta acgacgcaga
     7681 aatgcgaaaa ttacgaaagc aaaattaagt agta
//
LOCUS       DAJTYH010000159         7708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTYH010000159 DAJTYH010000000
VERSION     DAJTYH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..257
                     /locus_tag="OO609_003707"
                     /pseudo
     CDS             1..257
                     /locus_tag="OO609_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(287..3199)
                     /locus_tag="OO609_003708"
     CDS             complement(287..3199)
                     /locus_tag="OO609_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634962.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3202..3318
                     /locus_tag="OO609_003709"
     CDS             3202..3318
                     /locus_tag="OO609_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6634963.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3682..4524
                     /locus_tag="OO609_003710"
     CDS             3682..4524
                     /locus_tag="OO609_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6634964.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4527..5615
                     /gene="capU"
                     /locus_tag="OO609_003711"
     CDS             4527..5615
                     /gene="capU"
                     /locus_tag="OO609_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6634965.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5620..6570
                     /gene="virK"
                     /locus_tag="OO609_003712"
     CDS             5620..6570
                     /gene="virK"
                     /locus_tag="OO609_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6634966.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6635..7579
                     /gene="lpxM"
                     /locus_tag="OO609_003713"
                     /gene_synonym="msbB"
     CDS             6635..7579
                     /gene="lpxM"
                     /locus_tag="OO609_003713"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6634967.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2079 a         1715 c         1819 g         2095 t
ORIGIN      
        1 cccggtcaaa acagcgttgc ggtgcaccac gcctgacgga tgaactgcgt gctcagggtt
       61 accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg agggcaaagg
      121 cctcccggaa gttcagcccg gtcagttacc gcgcacatgg cctgcccgtg tcagaaaatc
      181 tgctggagca ggatttttac gccagtggtc cgaaccagaa gtgggcggga gacatcacgt
      241 acttacgtac agatgaagtt cggttacatc ctgtcagcac ggaacctcac gctttttgag
      301 cggcacgacg gatccccccc acccagtatc cgacagactt ttcactgcgt tcgtaaaaac
      361 ccttcttccc tcccagcata tccactaatt ccggtgacat atcctgcaga taagtctgca
      421 aatcatgccc cagatcttcc ggtgactgca actcataaac cgcgcgccac acatcaccct
      481 tccttatttt aagccccggg ttaagaatat actgtccgtg gttcagcctc agaaaaagct
      541 tttttccata aggatttttg ctgttaactt catgttgaga gagctggctg ctgatataac
      601 tccttcgttt gcggcgttcc ggcagcaacg cttcaggaag atgaaccaca cactctgcca
      661 gtcgtgccgc actgaaggct tccgaaaaaa acagagcact tcccagatgc gtgtggaaca
      721 gcgcctgcat caggatcacc agaaacattg ccatctgccg gttgtccagt ttgatgaggc
      781 gtccttccac ctgcagtgac agactttcag gtttcagcag ggtatacaac tgcgcacttt
      841 tctgttgcgc ataacggggc gtactgaccg ccgctgacag aacttgatga taagccgcca
      901 gcccgtcatt accggtcagc aagggatctg caccacgctc cagcagtaac tgaaccagtg
      961 caatattacc tgcccgggct gctgacatca gcggagtaag attaaacggc gaacggtgat
     1021 caacaccata acgttcaata tcccgcaaca cggcaaccgg gttattcatt gagtatggcc
     1081 ggaaatactt cagctccagt aactgcagag tcttcgttct gggctgcctt gtgggcttaa
     1141 aatcttcccg gtacagtgca ttatacagcg ggaacaggga ataaatcaga ctgtactcca
     1201 gcgcctgcag ttgtatggtt ttatcggctg tacctgcgag gatatgctgt ttccactgtc
     1261 gtgcatcctc tgcggtgata atctgccatg gcatctcaga tgtctgcagg atccggctac
     1321 ggatggtgtc cgcctgctcc tgcttcccct gtttttcaag cagatgtgcg gttttctgcc
     1381 actcatcacg gctggaaatc tcttcctgca gattcagagg ctcttcctga tgcgtgagcg
     1441 caagcagact ccacagagga tgctccacct gctgttcaac aagatacaca ttgtgtttag
     1501 cacgggtgag cgcaacatac agcgcgttgg taaaaaattt atagatttcc gctgaacgat
     1561 cctgcctgtc ccgcggacgg gaataacggg cctcgccctc aagatcagcc ggtgtaagac
     1621 cctcgcaaat atcatcaaat gcctgccggg cagcgcttac tatattgtaa aggatcaccg
     1681 tttcgtattc cagcccctta acctcctgaa ccgaaaagac cagcggcgta ctgaaccagc
     1741 atcgcgcctg tgctttctgt tccggatgca tcacaatgac cgctacacga ttcgaaagtg
     1801 aggttttggc attcagttcc tgctttgtct cttccctatc gtccagcaac cggatggtcc
     1861 cttctgcctg accgcagctt ctgacaaaat ggtgactttc ccgatcaata gcactaaagc
     1921 ggacctgttt gagtctcagc aaccggttag ccagtgcagt gacgtggtgc ccgttacggt
     1981 aattcgcctg caggatatta acggttgtgt cgtttccctg ttgctgccgg aaaaagagac
     2041 ttttgagcga actccatgag aaaaagctcg gatgaactat ctgattggca tcaccacaga
     2101 gtaaaaactg accgggatga cgtagcgttt tcagtaccag ttgcagttga acaggcgtca
     2161 tatcctgaac ttcatcaaca aaaatgacat cataacgggg agttgccaac gaaagatact
     2221 gatgactgag cagattactg tcatactgat gcgactgttt cagccatgcg atataacgtt
     2281 caaacaggac atagaccgtc gggcggtcct ccatgccata gagcgactgc cgtatcccca
     2341 gtgacagata cgcctcccgg gacagcggcc cattcccgga ggcaaccgca ccgatcacgc
     2401 ccctgaactc ctcatacagg gtatgggcag cccccagtgc ggcgatagcc ctgttgcggg
     2461 gcagccagtc gctgaacgca ctgaaagtca cctccctgcc ttccggaatg cgcagagttt
     2521 ccagaaattc cgtcagcgaa agaaagtcaa tattctgtac ctcactccct tcccctgaag
     2581 cgtcatagag agtacgcgct ttttccacca gaaatgagga aaggctgaga tacagtatat
     2641 caccagcagc ctgcttcatt ttttccagta ccagcgccgt cttaccactt cccgccgagc
     2701 caacaatcac cagcggtagc gggtgttcgt acagtgcctg ctgatcatca tcaaaggaca
     2761 gcattttatc caggcggtgg aaacgcccat gagacggatt gatatacgtc agcgcttcag
     2821 tcgcgatatc aacaggatcc ggcactggtt gttgttgtaa gcgcccttca tcaatcacca
     2881 cgtttctgcg aagaaaacgt gaagtgttat aggcatgatt gcgaatatat tccagaacaa
     2941 ggcagatagt ttcaccgtgg tagcgataca gcgaaaacag taatcggtcc cggatactga
     3001 gccgggcgcg gtaaagattg gtgtcaattt tacgaacatc agcctgaaca aagttatcgg
     3061 tggcaatagc ctgtgcaaat tttttataac ccgggatcgt gtcaggattc agagtatgat
     3121 aaagacagat tttcatggct gccggtttcc gtcgtgcaaa aaacatgctg attttacgaa
     3181 ggaatgcctt actgaacaac cttgcccgga ataccccccg aaatcagatg gaacaatcag
     3241 aaaaatgctc tgcagttatg gaataaagaa ggtggggtgg tacgccggga gggcgatccg
     3301 ctggaaatgg cgcagtagct gcaggcctgt catgatgcag gaatagaagt tcgtgtacag
     3361 gttaatgaaa gcgtcacccc gtaaataaac tctgcgctga aattcctttt tttcattgga
     3421 gtacctgtgt tgttctgagg tgaacatatc acctctgttc aggtagccaa attcagtgtg
     3481 ccacctcaag gttcttagtg agtcgttttt aaaagatatg ataatatatc attgtttatg
     3541 aattgttgat gtttaattta gtaaattata tcaaatatgc catgggtttg ctatgttatt
     3601 catccagtat ctggaaataa aatgcaccag ctttattggg ttttcctttt gtctctgctt
     3661 gctaggtgcc agaaacttta aatgttaaac gaagggggca ttttgttcaa agccaatcat
     3721 gttccggtat taatgtatca tcatgtcagt cattgccccg gactggttac actttctccc
     3781 gtgacattcc ggaagcaaat aaaatggttg gctgaaaaca actggaaaac actgtcatca
     3841 gatgaactgg agttttttta tcggggcgga aaactccccc ggaaaagcgt tatgttgaca
     3901 tttgatgatg gataccttga caactggttt caggtatatc ctttattaaa agagtttaat
     3961 ctcaaagcac atattttttt aattaccggt tttattggga atggtccggt tcgtcactct
     4021 cctggaaagg aatattctca tcgcgattgc gaacatcaaa tagcgacagg gaatgctgat
     4081 aatgttatgc ttcgctggtc tgaagtaaac gaaatgttgc aatctggtct ggtcgaattt
     4141 catgttcata cgcatactca tacacgctgg gataaaaagt tcagttcacg agaagaacag
     4201 tgtaaacatc ttcggcagga cttgttgagc gggagggaat atttgaaaga aatgacagga
     4261 aaatgcagta aacatttgtg ttggccggaa ggttattata acaaggatta tattcaggtc
     4321 gctgaagaac ttggattcta ttatttatat acaacagaaa gaagaatgaa tgctcctgct
     4381 aaaggaacta cacggatagg aagaataagc accaaagaac gggaaagttg cgcatggctg
     4441 aaaagacgac ttttttatta tacaaccccg tttttttcat cgttgctggc ctttcataag
     4501 gggccaagat taccggatga ttaataatga atatactatt tacggaatca tcaccgaata
     4561 taggtggtca ggaattacag gctgttgctc aaatgaaggc cctgaagaaa atggggcatt
     4621 cagttctgct tgtctgcagg gaaaacagca aaattgcttt tgaagccagt aaattgggga
     4681 ttgatatcac attcgcgtta tttcgaaaca gtcttcacat ccctactgca tggagattac
     4741 tcggaatagt tcatggtttt cagcccaatg caatcgtttg tcacagtggg catgatagca
     4801 atattgttgg tttagtacgg ttattcacta ggaaacatcc attcagaatt atcaggcaaa
     4861 agacatattt gacacgaaaa acaaaagttt tttcaataaa tcatttttgc gatgaggtga
     4921 ttgttcccgg aacaagtatg aagacacatc tggagcagga aggatgtcga acccgggtta
     4981 ctgttgtgcc tccaggcttt gatttccaga aattatacgt tgattcccga aacagtttgc
     5041 ctccaaatgt tctttcttgg ctggcgtccc gaaggggatg ccccgttatt gctcaggtag
     5101 gaatgttgcg cccggaaaaa gggcacgaat ttatgttgaa tttactgttc catttaaaaa
     5161 tgaatggacg acagttctgt tggttgattg tggggtctgg ttcgcctgaa ctgcgggagc
     5221 atttacagta tcagattgac agtatgggca tgcatgatga tgtttttatt gctgacaatg
     5281 tttttcctgc cgcccccgta tatcgggttg ccagtctggt ggttctgcct tcagaaaacg
     5341 aatcgtttgg tatggtactg gcagaagcat cggcattttc tgtgcctgta ctggccagtc
     5401 agattggtgg aatccctgat gttattcaga ataaccagac cgggacattg ttaccagcag
     5461 gtaataagca cgcatggatg tgcgccctga atgatttttt taatgaccct gggcgttttt
     5521 atcagatggc tcgccaggca aaacaggata tagaagagcg gtttgatatt aataaaactg
     5581 cgttaaaaat actcacatta gcgaagcaca agtaacaata tgttttctgt aagtaactta
     5641 tcatttatcg gtttccttaa aaggattgtt ttttcctcag attcactccc ggggaagtgg
     5701 gaacacagaa aattccggtt catgtacatt ttgcgatgtg ctataaatcc ggttgccagt
     5761 attcgatatt attacgaact gcgttccttg cagtgcattg aggatattct ggcaatacaa
     5821 cccacgttgc cagcaagaat tcatcgacct tatttgcata aaggggggcg agcctggtcc
     5881 cgggggcaat atattcttga gcattatcgc tttgtgcaaa atttgccaga aaaatattct
     5941 gaatttttgt ttccacaaaa atcggtatca ttagtccaat tcatcgggaa agatggagag
     6001 gattttgata tccagtgttc tcccagtggt tttgaccggg aaggagagtt gatgttgtca
     6061 ttgtttttca ataaaatagt gattgcccgg ttaacatttt cagtcattct gactcaaaat
     6121 ggacacaccg cttttattgg cggattacag ggagccccca aaaataccgg acctgatatt
     6181 attcggtgcg caacccgggc ctgttatggg cttttcccaa aacggattat ttttgaagca
     6241 ttttgtgcgt tgatgaaagc ttgtaatgtc tctgagtgtc tggcagtcag tgagcacagt
     6301 catgttttcc ggcaattgag atactggtat cagaaacgca aaacctttgt tgctgtctac
     6361 agtgatttct gggagtctgt agcagggaaa acctgtggtg actggtacaa attaccaaca
     6421 caggtggttc gaaagccgct aagcaatata gctagtaaaa aacgctctga gtatcgaaaa
     6481 cgatacgctt tgctggatta tatccatgaa actgctatcc gctctttgga tgcatatcct
     6541 gtaaactcag aacatcagga cttaaattaa ggtttgatgt attctctgaa taaaatatta
     6601 atgatgattt tggtaggggc attcgcacta aataatgaaa aaatacaaat ccgagtttat
     6661 tcctgaattt aagaaaaatt atctttcccc tgtttactgg tttacatggt tcgttttggg
     6721 aatgattgca ggtatttcaa tgtttccccc ttcattcaga gatcctgtct tggccaaaat
     6781 agggcgttgg gtgggcagat tgagcagaaa agctcgtcgc agggcgacga ttaatttatc
     6841 gctttgtttc ccggaaaaga gtgatacaga acgggaaata attgtcgaca atatgtttgc
     6901 cacagcattg caatccatag tgatgatggc agaactggca attcgtggtc cggaaaagtt
     6961 ccagaaacga gtgttctgga aaggcctcga aattcttgag gaaattcggc acaataacag
     7021 aaatgtgatt tttctggttc cccatggctg gagcgtggat attcctgcaa tgttgctggc
     7081 agctcagggg gaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta
     7141 tgtctggaat tctgtacggc gtaaattcgg ggggcgctta cattcccggg aggatgggat
     7201 aaaaccattt attcagtcag tacgccaggg atactggggg tattaccttc cagatcagga
     7261 tcatggtcct gaatacagtg aatttgctga tttttttgcg acctataaag cgacattacc
     7321 aattattgga cgtctgatga acatcagtca ggctatgatt ataccgcttt tcccggttta
     7381 tgatgaaaaa aaacatttcc tgactattga agttcggcca ccaatggatg catgcattgc
     7441 cagcgcggac aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg
     7501 gtcacatccg gaacagtata tctgggtttt aaaattgtta aaaacgcgca aatcaaacga
     7561 agcggacccg tacccttgaa attataaagt acaggtattt ccactagttg tttcttacag
     7621 gttaccaatc gaaacacatc ccccttccgt gtgttgcccg tttggcacgg aggactgtgt
     7681 agatgcagtt cgttaaattt aatttatg
//
LOCUS       DAJTYH010000160         7469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTYH010000160 DAJTYH010000000
VERSION     DAJTYH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..685
                     /locus_tag="OO609_003714"
     CDS             <1..685
                     /locus_tag="OO609_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6634968.1"
                     /translation="AICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKTTNQ
                     IHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELES
                     ELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYST
                     GGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVVACA
                     LANKLARIA"
     gene            complement(1024..1794)
                     /locus_tag="OO609_003715"
     CDS             complement(1024..1794)
                     /locus_tag="OO609_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634969.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1839..2273)
                     /locus_tag="OO609_003716"
                     /pseudo
     CDS             complement(1839..2273)
                     /locus_tag="OO609_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2285..2506)
                     /locus_tag="OO609_003717"
     CDS             complement(2285..2506)
                     /locus_tag="OO609_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634970.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2507..3190)
                     /locus_tag="OO609_003718"
     CDS             complement(2507..3190)
                     /locus_tag="OO609_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6634971.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3254..3565)
                     /locus_tag="OO609_003719"
     CDS             complement(3254..3565)
                     /locus_tag="OO609_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6634972.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3562..4464)
                     /locus_tag="OO609_003720"
     CDS             complement(3562..4464)
                     /locus_tag="OO609_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6634973.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4904..5863
                     /locus_tag="OO609_003721"
     CDS             4904..5863
                     /locus_tag="OO609_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6634974.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5863..6258
                     /locus_tag="OO609_003722"
     CDS             5863..6258
                     /locus_tag="OO609_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6634975.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6441..6821
                     /locus_tag="OO609_003723"
     CDS             6441..6821
                     /locus_tag="OO609_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6634976.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6867..7469
                     /locus_tag="OO609_003724"
                     /pseudo
     CDS             6867..7469
                     /locus_tag="OO609_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         2023 a         1935 c         1825 g         1686 t
ORIGIN      
        1 agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa gaactgagac
       61 ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag ataaggtgaa
      121 aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc cgacagggga
      181 tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg aatatctgag
      241 ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa tagcagagct
      301 ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca ttatgacaat
      361 tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg acgggaaaca
      421 gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc agtatagcac
      481 tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt tacgccggtt
      541 actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg gaaaacttgc
      601 tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct gcgctctggc
      661 aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat atcaggcctg
      721 atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata caagttaccg
      781 tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct gattaaaaac
      841 ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc aggtgcggaa
      901 ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta agcaagccca
      961 ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc cccgccatcg
     1021 tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg cccgggcttc
     1081 ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt catccggtat
     1141 acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat cgctgaccag
     1201 atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga gcaccacggg
     1261 caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca cgaccaggcg
     1321 aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg ccgcccggat
     1381 acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc gcaccgcatc
     1441 aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga aagacacctg
     1501 cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca gaacaagttc
     1561 cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca gcacgccatc
     1621 gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt aaatgctgat
     1681 ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc agccctgcgc
     1741 cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat tcataattca
     1801 tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc gtgtgtgttg
     1861 tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac gtattccggg
     1921 agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg ttcagcgatc
     1981 tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc gcgcacgttg
     2041 ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta gtcttccggg
     2101 atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga aatatccatt
     2161 ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag cggcgtgtct
     2221 cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta catactctct
     2281 cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc tccatcaaga
     2341 ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg tcagcattaa
     2401 agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg gcattggcca
     2461 cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac gcagcctccg
     2521 gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg gcagccagtc
     2581 gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac cggcgtccgg
     2641 actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc actatcgcat
     2701 tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt tccgtcgggt
     2761 gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt tgtggcagac
     2821 gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca tatgcggcct
     2881 tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc gcatttttcc
     2941 aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc agcgcgtctt
     3001 ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca gttttatcac
     3061 cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga tcggtgagga
     3121 tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta ccgaggacaa
     3181 aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc gttccccgct
     3241 gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa taccgcagcc
     3301 gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt tcatcagaaa
     3361 agggcgacgg gctggcgtac actgtcaccg acaccagccg ggagcggtaa aagcgcccgt
     3421 cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc gccgcgtcag
     3481 ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa cgggcggttg
     3541 cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga ctatccacgc
     3601 aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt caagttcccc
     3661 ccacagcaca ttgacgagct ctccacgctc atacaactgc cagccacaga aatcacagcc
     3721 ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt ctgcaataac
     3781 gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt tttctccctg
     3841 actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt ccgtatacca
     3901 gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca cttcaacatc
     3961 caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg gcactacgtt
     4021 gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt cacaccagtc
     4081 acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg gtaatccttc
     4141 ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca ggcagttttg
     4201 ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg cgatattttc
     4261 cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag agcggatatt
     4321 ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac cttcatttgt
     4381 ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct cagcgatctg
     4441 tgccggctca ccaggaaaat acatacgatt cgagcaccag tttggcatgg gaattctcct
     4501 ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg catacgaatg
     4561 caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg accgggtgag
     4621 gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg ccggcacggc
     4681 ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat aagaactcaa
     4741 aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt gacatagact
     4801 caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga cataaaggat
     4861 gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa aagtatcatg
     4921 cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg taagaaccag
     4981 tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg ccggcaaagt
     5041 ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca ctgctgtcat
     5101 aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca tacatcatgc
     5161 actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat tacctgtgac
     5221 cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa aaaaggcaaa
     5281 tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa aagttaatgt
     5341 aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag taaacaaact
     5401 tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt taattctcgg
     5461 tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat cccgcatcac
     5521 tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt ttattgctga
     5581 tgagttaata aaaaacagac acgataatga atatctccag acattaataa atgatgtagc
     5641 tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg cagaaagcat
     5701 caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg gcggaggagc
     5761 cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga acaaggtgga
     5821 tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag cagtgatcca
     5881 aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca ggacaacctg
     5941 acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta cagacaatcc
     6001 cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct aatcagcgtt
     6061 tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca aacaacagga
     6121 tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc atcttcagaa
     6181 aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag acgcaatctt
     6241 tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc tcttctttta
     6301 tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa tgcaacagac
     6361 aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca ttattgtcag
     6421 tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt gatgatgata
     6481 taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact caggctattg
     6541 cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc acctcaattg
     6601 taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa gctcttgcag
     6661 tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa gcaaagctaa
     6721 tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa ataggtcttc
     6781 tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag ttatagtaac
     6841 tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat gaggcatgga
     6901 ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga cgcccatggg
     6961 ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccagt gcaccatggc
     7021 gcgatttacc cgaacgatat ggggcatgga aaactgttta taaccgcttt aaccgatggt
     7081 caaagtcagg cgtgattaat attattttca acaggttgct ttcgctactt gatgcaaacg
     7141 gctttattga ctggtctgcc accgcgctgg atggcagtaa tatccgggcg ctgaaatgtg
     7201 ccgccggtgc tcaaaaaaac atcccgatat cgacggagat aatgggccgg gtcgctctcg
     7261 cggcggtttt ggcaccaaaa tccatctggc aacagacgga agtggcctcc cgttaaatat
     7321 cgtgctaagc cccggacaag ctcatgaaag ccagttcgcg caacgacttc tggacgggat
     7381 tggcgttcag cgtcagaacg gcagcatgaa acgccgtgga catgcggtac tggctgataa
     7441 agcgtattca gggcgcgcat tgcgcaatg
//
LOCUS       DAJTYH010000161         7400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTYH010000161 DAJTYH010000000
VERSION     DAJTYH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..782
                     /gene="ydfJ"
                     /locus_tag="OO609_003725"
     CDS             <1..782
                     /gene="ydfJ"
                     /locus_tag="OO609_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6634977.1"
                     /translation="ASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQS
                     KSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLG
                     WLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFAL
                     ENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYS
                     VIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            871..2331
                     /locus_tag="OO609_003726"
     CDS             871..2331
                     /locus_tag="OO609_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6634978.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2367..2570)
                     /gene="ydfZ"
                     /locus_tag="OO609_003727"
     CDS             complement(2367..2570)
                     /gene="ydfZ"
                     /locus_tag="OO609_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6634979.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2748..3434)
                     /gene="ydfH"
                     /locus_tag="OO609_003728"
     CDS             complement(2748..3434)
                     /gene="ydfH"
                     /locus_tag="OO609_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6634980.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3523..4269)
                     /gene="ydfG"
                     /locus_tag="OO609_003729"
     CDS             complement(3523..4269)
                     /gene="ydfG"
                     /locus_tag="OO609_003729"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6634981.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4406..6451
                     /gene="dcp"
                     /locus_tag="OO609_003730"
     CDS             4406..6451
                     /gene="dcp"
                     /locus_tag="OO609_003730"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6634982.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6496..7014)
                     /locus_tag="OO609_003731"
     CDS             complement(6496..7014)
                     /locus_tag="OO609_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6634983.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7290..>7400
                     /gene="ydeI"
                     /locus_tag="OO609_003732"
     CDS             7290..>7400
                     /gene="ydeI"
                     /locus_tag="OO609_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6634984.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAI"
BASE COUNT         1856 a         1738 c         1859 g         1947 t
ORIGIN      
        1 tggcgagcgt tgtcgtgatg gtctttgcta tctggttgcg tatgaatctg aaagaaagcc
       61 cggtctttga gaaggttaac gacagcaacc aaccgacagc aaaacctgca cctgctggta
      121 gcatgttcca gagcaaatcc ttctggctgg caacagggct gcgttttggt caggcgggta
      181 actccggttt aattcagact ttccttgcag gctatttagt acagacgtta ttgtttaaca
      241 aagcaattcc aacagatgca ttgatgatca gttcgattct cggctttatg accattccgt
      301 tccttggttg gttatccgat aaaattggtc gccggatccc gtatattatt atgaatacct
      361 ctgcgattgt gctggcgtgg ccaatgcttt ctatcatcgt agataaaagc tatgccccga
      421 gcaccattat ggttgcactg attgtgattc ataactgtgc ggtgctggga ttatttgctc
      481 tggaaaacat taccatggca gaaatgttcg gctgtaaaaa ccgcttcacc cggatggcta
      541 tttcaaaaga aattggtggt cttatcgctt ccggttttgg tcctatcctg gcgggtattt
      601 tctgcaccat gacggaatcc tggtatccga tcgccattat gatcatggca tattcagtga
      661 ttggtttaat ctctgcgctg aaaatgccag aggtgaaaga ccgtgattta agtgcgctgg
      721 aagacgctgc ggaagatcaa ccgcgtgttg taagagctgc gcaaccttcc agaagtctgt
      781 aaaccctcaa tcccttctct taccggagag gggattttta ttcatataac aaaacatata
      841 gcttgccata tttaaattta aggaattgtt atgggaaata atttgttatc agcaaaagcg
      901 acactccctg tttatgatcg taataacctg gccccaagaa ttgttcattt aggctttggt
      961 gcatttcacc gtgcgcatca gggcgtgtat gccgatattc ttgctacaga acatttcagt
     1021 gactggggat attatgaggt caacttaatc ggcggcgaac agcaaattgc cgatttacaa
     1081 cagcaagata atctttatac cgttgcggaa atgtcggccg atgcgtggac ggctcgcgtc
     1141 gttggcgtcg ttaaaaaagc cttgcacgta cagatggatg gcttagaaac cgtgttggct
     1201 gcgatgtgtg aaccgcaaat cgcgattgtc tctctgacaa tcaccgaaaa agggtatttc
     1261 cactctccgg cgaccggaca gttaatgctc gagcacccga tggtcgctgc cgacgtgcaa
     1321 aatccccacc agccgaaaac agcaacaggg gtgattgttg aggcgctggc tcgccgtaaa
     1381 gcggcaggac ttcccgcatt taccgtcatg tcatgtgaca acatgccaga aaacggtcat
     1441 gttatgcgtg acgttgtcac ttcctacgcg caagccgttg atgtaaaact ggcacaatgg
     1501 atcgaagata acgtgacttt cccatcaaca atggtggacc gtattgtgcc cgcagtgaca
     1561 gaggatacgc tggcgaaaat cgaacaactt accggtgtgc gcgatcctgc tggcgttgcc
     1621 tgtgaacctt tccgccagtg ggttatcgaa gataactttg tagccggacg tccggaatgg
     1681 gaaaaagcgg gagccgaact ggttagtgat gtgctgcctt atgaagagat gaagttgcgc
     1741 atgctcaacg gcagtcattc attcttggca tatctgggtt atcttgctgg atatcagcac
     1801 attaatgact gtatggaaga tgaacattat cgtcatgcgg cgtatggcat gatgttgcag
     1861 gaacaagcgc cgacgctgaa agtgcagggc gttgatttgc aagattacgc taaccgatta
     1921 attgcacgct atagcaaccc ggcgttacgt catcgaacct ggcagattgc gatggatggc
     1981 agccagaaat tgccacagcg gatgttggat tctgttcgct ggcatctggc gcatgacagc
     2041 aagttcgatc tgctggcgct gggcgtcgcg ggttggatgc gttatgtcgg tggtgttgat
     2101 gaacagggaa atccgataga aatcagtgac ccactgttac ctgttattca gaaggctgta
     2161 caaagtagtg ccgaagggaa agcgcgcgtc cagtcattgc tggcgattaa ggcaatcttt
     2221 ggtgatgatt tgccagacaa tagcttgttt actgcaaaag tgacggaagc gtacttgtct
     2281 ttattagcgc atggcgcgaa agcgaccgtg gcgaaatatt ccgtgaagta aaaaaagcaa
     2341 tgccgccatc ggcggcattc acacacttag ttcatgccga gacgaatcag gtccagaggt
     2401 gccagtttct cttcacaacc ttcaacaact acggtttttc cgcggcggat ttgctcagcg
     2461 gtcagacctt cagtatcaat cgacaggatc ttgccagtgt gaccggtgcc gctaaccata
     2521 acacggctgc cagtggtgat tgcgttacgg ttacgatcgt aggtcgtcat aggtgttttc
     2581 tcctttctga tttacagtcg aagcccacgt aacgggcgca atataaatac tcctgtcgag
     2641 tgaaatattt tttgtttttg atcaagctca catctttttt gatgcatgca tgagccgtaa
     2701 ggcgggggga aaagcctctc atcccggatg ggatgagagt aaatgaatta ctcttcgcta
     2761 aaccagtcgc tgttttcctg gcggatctgg cgcacggatt cgctgatttc ctgcaaatgt
     2821 tgcgtcattg cacgttctac cgcatcgcca tcacgttttt gcagggcaga gaaaatatca
     2881 agatgctggc gtaacagcat ttctggtgga gaaacatggt cgaaactcat atagcgcacg
     2941 cgatcaacgg tcgctttcag gttctcaatg gtatcccacg ccagttgaca gtcggcaatc
     3001 tgcgtcagga gttgatggaa gttgtcatca agttcaaaaa aatcatccag ttgcttgcgc
     3061 tcaatggcaa tgcgttgctg gtgaagattt tgttccagtt ggtagcactg gctttcggta
     3121 atcatgctcg ccgcccgacg cgccaccgcg cactcaatgg cctgacggat aaaactgccg
     3181 ttgcgcacct gggccatgga aattttgttg acgtagctgc cacgctgcgg acgaatttga
     3241 atcaggccgt tttccgccag tttaataaag gcttcacgaa ccggctggcg tgacacattg
     3301 aaacgaacag aaacttcttt ttccgacaac ggtgtgcctg gtgcaatcag gcaatggaca
     3361 atgtcgcgac gaagaatacg ataaatctgc tgattgacag gctgtgtggg attaagttgc
     3421 gtttcgacgg tcattcgttc ttgcttgtga gtgagttaac tgcatgagtc taccactttt
     3481 ttgtgacaag caataacccg gcagttacgc cgggtataaa aattactgac ggtggacatt
     3541 cagtccggca tagctttggg taaccggcat catttccagg gtattgatat tgacgtgagc
     3601 aggcagcgtt gacacccacc agacggcttc gctgacatct tctggcgtca atgcaacggt
     3661 attttgatag gttttttccg ctttaccgtc atcgccttta aagcggacat tggaaaactc
     3721 ggtgccaccc accagacccg gttcgatgtc ggtgacgcgc accgccgtac catgcagatc
     3781 cgtacgcaga ttcaggctaa actgacgaac aaacgctttc gtcgcaccgt aaacgttacc
     3841 accggcatac ggccagctgc ctgccgttga gccaatgtta ataatatgac catgattacg
     3901 ttcaaccata cccggtaaga cggcgcgcgt catatatact aggcctttgt tgttggtatc
     3961 aatcatcgtt tcccagtctt caacgctggc tttatgcgca ggctccatgc ccaacgccag
     4021 gccggcatta tttaccagga tatcaatatt gctccactcg gcagggagcg atgccagcat
     4081 ctcttcaata gcggcgcggt tgcgaacgtc cagttgggcg atatacagat tatctcccag
     4141 ttcgtctgtt aactcctgca accgctcctg gcgacggcca gtggcgataa ctttatgccc
     4201 ttgttgaata aaacgacgag taatgcattc accaaaacct gccgttgctc cagttactaa
     4261 aacgatcatc tcactgttcc tcaacgcttt tgtgtgtcaa taacatagca cgtgatgctt
     4321 aagagcgggt aatactggtt tgcaagattg cactccgtta tcagtaagcc tacgctatgg
     4381 aacacctcgc gttcaggaga agaaaatgac aacaatgaat cctttccttg tgcaaagcac
     4441 actgccgtat ctggctcctc attttgatca aattgccaat catcactatc gcccggcatt
     4501 cgatgaggga atgcaacaaa agcgggcaga aattgctgcc atcgcgctta acccgcaaac
     4561 gcctgatttc aacaatacta ttctggcact ggaacaaagc ggagaattaa ttacccgcgt
     4621 taccagcgtc ttttttgcga tgactgcggc gcataccaat gatgaattac agcgtcttga
     4681 tgaacagttt tccgctgaac tggcggaact ggctaatgat atctatctga acggtgaatt
     4741 attcgcgcgg gtagatgctg tctggcagcg ccgtgaatcc ctggggcttg atagtgaatc
     4801 catccgcctg gtggaggtta ttcatcaacg ttttgtcctt gccggagcca aacttgcaca
     4861 agctgataaa gcaaaattaa aagtactgaa tacagaagct gcgaccctga ccagccagtt
     4921 taaccagcgg ttacttgcag caaataaatc cggcggtctg gttgtgaacg atatcgcgca
     4981 gctggcagga atgagtgagc aagagattgc gctggcggca gaggcggctc gcgagaaagg
     5041 tctggataac aaatggctga ttccgctgct gaataccacc caacaaccgg cgcttgccga
     5101 aatgcgcgat cgtgcgacgc gtgaaaaact gtttattgcg ggctggacgc gagcggaaaa
     5161 aaatgatgcc aatgataccc gcgctatcat tcaacgtctg gtagagattc gcgcacagca
     5221 ggcgaaactg cttggttttc ctcattatgc cgcatggaaa atcgccgatc agatggcaaa
     5281 aacgccagaa gcagcactca actttatgcg ggaaattgtt ccagcggcgc gtcaacgtgc
     5341 gagcgatgaa ttagcctcca tacaggcggt tatcgataag cagcagggcg ggtttagcgc
     5401 gcagccgtgg gactgggcat tttatgcgga acaggtacgg cgggagaaat ttgatcttga
     5461 tgaggcgcag ctcaagccat attttgaatt aaacacggtg ttaaatgaag gtgtattctg
     5521 gaccgcgaat cagctcttcg gtattaagtt tgtcgaacgt tttgatattc cggtctacca
     5581 tcctgacgtt cgtgtgtggg aaatttttga tcataatggc gtgggactgg cgttatttta
     5641 cggtgatttc ttcgcccgtg attcaaaaag cggcggtgca tggatgggca attttgttga
     5701 gcaatcaacg cttaatgaaa cgcatccggt aatttataac gtctgcaatt atcagaaacc
     5761 cgctgccggt gagcctgcgt tgttactctg ggatgatgta ataaccttat tccatgaatt
     5821 tggtcatacg ctgcacggcc tttttgcccg ccagcgttat gccacgcttt ccggcaccaa
     5881 cacgccgcgt gattttgtcg aatttccgtc gcaaatcaac gaacactggg caacgcatcc
     5941 gcaggtattc gctcgctacg cccggcatta tcagagcggg gcagcaatgc ctgacgaact
     6001 gcaacagaaa atgcgtaatg ccagcctgtt caacaaaggg tatgagatga gcgaactgct
     6061 tagcgccgca cttctcgata tgcgctggca ttgcctggaa gaaaacgaag caatgcagga
     6121 tgtcgatgat tttgaattgc gggcgctggt ggcggaaaat atggatcttc ctgctatacc
     6181 gccacgctat cgcagcagtt atttcgccca tatttttggt ggcggatatg ctgcaggtta
     6241 ttacgcttat ctgtggacgc aaatgttggc cgatgatggt taccagtggt ttgttgagca
     6301 gggcggatta acgcgtgaaa atgggcagcg ttttcgcgag gcgatccttt ccagaggtaa
     6361 cagcgaagat ctggaacgcc tgcatcgaca atggcgtggt aaggctcctc agattatgcc
     6421 gatgctgcaa catcgtggct tgaacatata aacaccacca atggctggcc tggagatatc
     6481 gccaggccag cctggttata gcagtaattg cagcagctgg gcgagggcaa acctcaccgc
     6541 taaagctaat accgtttcgc agtcgccagc aaaatgcata accgcagtgt agttctggcc
     6601 tttaatatgc cacgcgaacc agacggtacc cgctggcgta ccatcttcac cgccctccgg
     6661 tccgccgtag ccggtaatgg caatactgac atcagcatcc gcacgctcta tggcacctgt
     6721 tgccatttct gctgccactt tctcactcac cgcagaatat cgttcaagag actgctggct
     6781 tacgctgagg attttcatct ttgcctgatc ggtgaaagta acaaagcctg caccgtaaaa
     6841 tttgggtgta tcttcagctg cacacagggc gctagccaac ttaccgccgg tgcatgattc
     6901 ggcggtagtt aagcgtaatt gatccgcgat aagtctttga ctcaacgcgg atgtcaggtt
     6961 ttcaatagtg tcggtatctg cgagttgtac aattttatct ttattaatgt tcactgtttt
     7021 ttgtccacgc tattatatgt aaaggtgatt tgctttttct gtatgctcgt ccaatataat
     7081 aattttcaaa catataaatc cgattattct gaaaacatgt ttaagactct tcaatcttct
     7141 taactttagt gagtcaaatt ctctgaatat ttgacagcta gtgggctgat tttaaatgcc
     7201 ttataacaat tggcagtatt cttatttggc aattataaaa atagcaacca gacttaataa
     7261 gtcaaaccaa acgcaattaa ggagttagta tgaaatttca gtccattgta ttagcaagtt
     7321 ttcttgtcat gccttatgcg ttggcagatg accagggcgg tttaaaacag gatgcagcgc
     7381 caccgccgcc tcatgcaata
//
LOCUS       DAJTYH010000162         7202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTYH010000162 DAJTYH010000000
VERSION     DAJTYH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..821
                     /locus_tag="OO609_003733"
                     /pseudo
     CDS             1..821
                     /locus_tag="OO609_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            826..1791
                     /gene="rbsC"
                     /locus_tag="OO609_003734"
     CDS             826..1791
                     /gene="rbsC"
                     /locus_tag="OO609_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6634985.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1816..2706
                     /gene="rbsB"
                     /locus_tag="OO609_003735"
                     /pseudo
     CDS             1816..2706
                     /gene="rbsB"
                     /locus_tag="OO609_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2832..3761
                     /gene="rbsK"
                     /locus_tag="OO609_003736"
     CDS             2832..3761
                     /gene="rbsK"
                     /locus_tag="OO609_003736"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6634986.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3765..4757
                     /gene="rbsR"
                     /locus_tag="OO609_003737"
     CDS             3765..4757
                     /gene="rbsR"
                     /locus_tag="OO609_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6634987.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4723..6150)
                     /gene="mdtD"
                     /locus_tag="OO609_003738"
     CDS             complement(4723..6150)
                     /gene="mdtD"
                     /locus_tag="OO609_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6634988.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6173..6865)
                     /locus_tag="OO609_003739"
     CDS             complement(6173..6865)
                     /locus_tag="OO609_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6634989.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1826 a         1751 c         1904 g         1721 t
ORIGIN      
        1 accgaagatt cgctgattga gatgatggtg ggccgcaagc tggaagatca atatccacac
       61 ctggacaaag cgccaggaga tatccgcctg aaagtcgata atctctgcgg acctggcgtt
      121 aacgatgtct cttttacttt acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc
      181 gcaggtcgta ccgaactgat gaaagtgctc tacggcgcac tgccgcgcac cagcggttac
      241 gtcaccctgg atgggcatga agtcgttacc cgttcaccgc aggatggcct ggcaaacggc
      301 attgtgtata tctccgaaga ccgtaaacgt gacggtttag tgctgggcat gtcagtaaaa
      361 gagaacatgt cgctgacagc gctgcgctac ttcagccgcg ctggcggcag tttgaagcat
      421 gccgatgaac agcaggctgt gagtgatttc attcgtctgt ttaatgtgaa aacgccgtcg
      481 atggaacagg caattggtct gctttccggt ggcaatcagc aaaaagtggc gattgcccgc
      541 ggtctgatga cacccccaaa gtattgatcc ttgatgagcc tacccgtggc gtagatgtcg
      601 gcgcgaaaaa agagatctat caactgatta accagttcaa agccgatggc ttgagtatca
      661 ttctggtgtc atcggagatg ccagaagtat taggcatgag cgatcgcatc atcgtcatgc
      721 atgaagggca tctcagcggg gaatttactc gtgagcaggc cacccaggaa gtgttaatgg
      781 ctgtcgctgt gggcaagctt aatcgcgtga atcaggagta aaaaaatgat aacccagact
      841 gtctctggtc gccgttattt cacgaaagcg tggctgatgg agcagaaatc gcttatcgct
      901 ctgctggtgc tgatcgcgat tgtctcgacg ttaagcccga actttttcac catcaataac
      961 ttattcaata ttctccagca aacttcggtg aacgccatta tggcggtcgg gatgacgctg
     1021 gtgatcctga cgtcgggcat agacttgtcg gtaggttctc tgttggcgct aaccggcgca
     1081 gttgctgcat ctatcgtcgg cattgaagtc aatgcgctgg tggctgtcgc tgctgctctc
     1141 acgttaggtg ccgcaattgg tgcggtaacc ggggtgattg tagcgaaagg tcgcgtccag
     1201 gcgtttatcg ctacgctggt tatgatgctt ttactgcgcg gcgtgaccat ggtttatacc
     1261 aacggtagcc cagtgaatac cggctttact gagaacgccg atctgtttgg ctggtttggt
     1321 attggtcgtc cgctgggcgt accgacgcca gtctggatca tggggattgt cttcctcgcg
     1381 gcctggtaca tgctgcatca cacgcgtctg gggcgttaca tctacgcgct gggcggcaac
     1441 gaagcggcaa cgcgtctttc tggtatcaac gtcaataaaa tcaaaatcat cgtctattct
     1501 ctttgtggtc tgctggcatc gctggccggg atcattgaag tggcgcgtct ctcctctgca
     1561 caacccacgg cggggactgg ctatgagctg gatgctattg ctgcggtggt tctgggcggt
     1621 acgagtctgg cgggcggaaa aggtcgcatt gttgggacgt tgatcggcgc attaattctt
     1681 ggcttcctta ataatggatt gaatttgtta ggtgtttcct cctattacca gatgatcgtc
     1741 aaagcggtgg tgattttgct ggcggtgctg gtagacaaca aaaagcagta ataacgacta
     1801 caggacatct tgaatatgaa catgaaaaaa ctggctaccc tggtttccgc tgttgcgcta
     1861 agcgccaccg tcagtgcgaa tgcgatggca aaagacacca tcgcgctggt ggtctccacg
     1921 cttaacaacc cgttctttgt atcgctgaaa gatggcgcgc agaaagaggc ggataaactt
     1981 ggctataacc tggtggtgct ggactcccag aacaacccgg cgaaagagct ggcgaacgtg
     2041 caggacttaa ccgttcgcgg caccaaaatt ctgctgatta acccgaccga ctccgacgca
     2101 gtgggtaatg ctgtgaagat ggctaactag gcgaacattc cggttatcac tcttgaccgc
     2161 caggcaacga aaggtgaagt ggtgagccac attgcatctg ataacgtact gggcggcaaa
     2221 atcgccggtg attacatcgc gaagaaagcg ggtgaaggtg ccaaagttat cgagctgcaa
     2281 ggcattgccg gtacatccgc agcccgtgaa cgtggcgaag gcttccagct ggccgttgct
     2341 gctcacaagt ttaatgttct tgccagccag ccagcagatt ttgatcgcac caaaggtttg
     2401 aacgtaatgc agaacctgtt gaccgctcat ccggatgttc aggctgtatt cgcgcagaat
     2461 gatgaaatgg cgctgggcgc gctgcgcgca ctgcaaactg ccggtaaatc ggatgtgatg
     2521 gtcgtcggat ttgacggtac accggatggc gaaaaagcgg tgaatgatgg caaactagca
     2581 gcgactatcg ctcagctacc cgatcagatt ggcgcgaaag gcgttgaaac cgcagataaa
     2641 gtgctgaaag gcgagaaagt tcaggctaag tatccggttg atctgaaact ggttgttaag
     2701 cagtagtttt aatcaggttg tatgacctga tggtgacata aatacgtcat cgacagatga
     2761 acgtgtaata taaagaaaag cagggcacgc gccaccctaa cacggtggcg cattttatgg
     2821 acatcccgaa tatgcaaaac gcaggcagcc tcgttgttct tggcagcatt aatgctgacc
     2881 acattcttaa tcttcaatct tttcctactc caggcgaaac cgtaaccggt aaccactatc
     2941 aggttgcatt tggcggcaaa ggcgcgaatc aggctgtggc tgctgggcgt agcggtgcga
     3001 atatcgcgtt tattgcctgt actggcgatg acagcattgg cgagagcgtt cgccagcagc
     3061 tcgccactga taacatcgat attactccgg tcagcgtgat caaaggcgaa tcaacaggtg
     3121 tggcgctgat ttttgttaat ggcgaaggtg agaatgtcat cggtattcat gccggcgcta
     3181 atgctgctct ttccccatcg ctggtggaag cgcaacgtga gcgtattgcc aacgcgtcag
     3241 cgttattaat gcagctggaa tcaccactcg aaagtgtgat ggcagcggcg aaaatcgccc
     3301 atcaaaataa gactatcgtt gcgcttaacc cggctccggc tcgcgaactt cctgacgaac
     3361 tgctggcgct ggtggacatt attacgccaa acgaaacgga agcagaaaag ctcaccggta
     3421 ttcgtgttga aaatgatgaa gatgcagcga aggcggcgca ggtactgcat gaaaagggta
     3481 tccgtactgt actgattact ttaggaagtc gtggtgtatg ggctagcgtg aatggtgaag
     3541 gtcagcgcgt tcctggattc cgggtgcagg ctgtcgatac cattgctgcc ggagatacct
     3601 ttaacggcgc gttaatcacg gcgttgctgg aagaaaaacc attgccagag gcgattcggt
     3661 ttgcccatgc tgccgctgcg attgccgtaa cacgtaaagg cgcacaacct tccgtaccgt
     3721 ggcgtgaaga gatcgacgca tttttagaca ggcagaggtg acgcttggct acaatgaaag
     3781 atgttgcccg tttggcgggc gtttctacct caacagtttc tcacgttatc aataaagatc
     3841 gcttcgtcag tgaagcgatt accgccaaag ttgaagcggc gattaaagaa ctcaattacg
     3901 cgccatcagc tctggcgcgt agcctcaaac tcaatcaaac acataccatt ggcatgttga
     3961 tcactgccag taccaatcct ttctattcag aactggtgcg tggcgttgaa cgcagctgct
     4021 tcgaacgcgg ttatagtctc gtcctttgca ataccgaagg cgatgaacag cggatgaatc
     4081 gcaatctgga aacgctgatg caaaaacgcg ttgatggctt actgttactg tgtaccgaaa
     4141 cgcatcagcc ttcgcgtgaa atcatgcaac gttatccgac agtgcctacc gtgatgatgg
     4201 actgggctcc gttcgatggc gacagcgatc ttattcagga taactcgttg ctgggcggag
     4261 acttagcaac gcaatatctg atcgataaag gtcatacccg tatcgcctgt attaccggtc
     4321 cgctggataa aactccggcg cgcctgcggt tggaaggtta tcgggcggcg atgaaacgtg
     4381 cgggtctcaa cattcctgat ggctatgaag tcactggtga ttttgaattt aacggcgggt
     4441 ttgacgccat gcgccaactg ttatcacatc cgctgcgtcc tcaggccgtc tttaccggaa
     4501 atgacgctat ggctgttggc gtttaccagg cgttatatca ggcagagtta caggttccgc
     4561 aggatatcgc ggtgattggc tatgacgata tcgaactggc aagctttatg acgccaccat
     4621 taaccactat ccaccaaccg aaagatgaac tgggggagct ggcgattgat gtactcatcc
     4681 atcggataac ccagccgacc cttcagcaac aacgattaca acttactccg attctgatgg
     4741 aacgcggttc ggcatagatt tacgctgtct tttgatcaaa ttattaccat cggttgtttt
     4801 cagaagcatg aacattgctg ctgaagcaac agtaataatg cccatcgtga taaacgtata
     4861 gtggaattgt tcgacagtcg ttgtgccttc catcccttca taaatgcgaa ggacggccgc
     4921 acttacagca acgcctaaac taatcgacag ttgctgcgtg accgccagaa cactgttacc
     4981 gctgctggcg ttgtcatcgg tcagatcggc aagtgtgatg gtattcatcg cggtaaactg
     5041 cgtcgacata gccatcccta atataaacaa cggcaagatc agcatccata tcgccattgc
     5101 cggtgattgc aaagagaact gagcgatcat tagcccaata atcaccgtga tccccactaa
     5161 tgtatggcga tagcccagac gacgtaagac ttgggtaacc atcgattttg caataatgga
     5221 acctaacgct gtcggtgcca tcatacagcc ggcaataaac gcctgataac caaatcctac
     5281 ctgtaacatc aatggcataa ggaacggtac acagccggtc cccagacggg ttgcaatatt
     5341 gcctacgata ccgatcgaga aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt
     5401 tggcgtacgt cgcgcatgga gaatatagag aagcagtaac ccgatgctgg tgacaattac
     5461 cgtcaaggca atccagctgg cgacaatctt ttccccgaat agctctattc cgcttgaaaa
     5521 gagaacaagg ctgaggccaa acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt
     5581 ggtgaaattg ggcatatgtt tgcgcgcgta aagaaggccc gcaataccta tggggatatt
     5641 aattaaaaat atccagtgcc aggttgccca ggtgaccagc acgccgccaa gaacggggcc
     5701 taaaattggc cccaccagac ccggcatggc gacaaaattc aataccggaa gaagttcatt
     5761 acgaggataa gcacgcagta aggccggccg agcaacaggc atcatcattg cgtcgcctat
     5821 cccttgaata acccggaaga caaccagctg tggtagcgaa ttagaaagtg cgcaggccag
     5881 agaacccaac gtgaacagac tcacggcaag ggtaaaaatg cgacgcgtac cgaagcgatc
     5941 ggctagccat ccgcttaccg gaataagcat cgccaccgtc agcgtataac tgatgatggc
     6001 tgattgcatc gcgagaggag aacgattaag gctatgagcg attgcgggta aggcggtatt
     6061 cagaatagtg gcatcaagtg cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc
     6121 cgacatacta cgcttctttt tatcgctcat tcaatgtcct gttatcgggt tatcacttat
     6181 caggtgagcg tagcagcgcc tgacaagctt taaatgccgc gtcgccatcg ctttggataa
     6241 tcgcatcgac aatcgcctgg tgcagatcca gctttatcac tgtgtcgctg gtaattgacg
     6301 tgaagtaagt gtgataaacc gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg
     6361 cgctcatttc atagatatgc tcatgccagg ccatatcgac ttcgatccag cgttcacggc
     6421 gaaagttctc ttttaatgcc gccatttcgg ccattaacgt attgagatgc gccttctgtt
     6481 ccgcggtgcc aaccgttgct gccagtaggc aggcttgcgg ctccagacag atacgcataa
     6541 ccagaaagtg atcgatgacc tgatgaaagt tctcttctgt tatccaccaa gtaagcaatt
     6601 cctgatcaag aaaattccag ttcgattgtg gcatgacccg agtaccaatt cgcggtcgcg
     6661 gtaaaaccat cccttttgcc gttaacgttt tgaccgcttc gcgtaccgct gtacgactca
     6721 ctccaaattg ctcgcccagc tcaatctcac cgggcaaaat ggtgccgggt tcatattcac
     6781 cttttaagat ccgttgcgcc agcttctcag ccagaacata cgaaaggttt ttctgtgcag
     6841 ctaactgttg tgcgcttaaa ggcattactt atcttccttt ttctttttat tcctccttag
     6901 tatgccacca ggaagtgtga ttacggttgc aaaaacggca aattgcttgt tttatggcac
     6961 attaacgggg tgttttgctg aaaaaatgcg cggtcagaaa attattttaa atttcctctt
     7021 gtcaggccgg gataactccc tataatacgc caccactgac acggaacaac ggcaaatacg
     7081 ccgccgggtc agcggggttc tcctgagaat tccgacagaa aaagcaaaaa taaatgattg
     7141 actctgtagc gggaaagcgt attatgcaca cccccccccc gcgccgctga gaaaaagcga
     7201 ag
//
LOCUS       DAJTYH010000163         7188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTYH010000163 DAJTYH010000000
VERSION     DAJTYH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO609_003740"
                     /pseudo
     CDS             complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO609_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            764..916
                     /locus_tag="OO609_003741"
                     /pseudo
     CDS             764..916
                     /locus_tag="OO609_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2297..3244
                     /gene="icsP"
                     /locus_tag="OO609_003742"
     CDS             2297..3244
                     /gene="icsP"
                     /locus_tag="OO609_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6634990.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3806..4178)
                     /locus_tag="OO609_003743"
                     /pseudo
     CDS             complement(3806..4178)
                     /locus_tag="OO609_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4422..4541
                     /locus_tag="OO609_003744"
                     /pseudo
     CDS             4422..4541
                     /locus_tag="OO609_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4791..5657
                     /gene="ospB"
                     /locus_tag="OO609_003745"
     CDS             4791..5657
                     /gene="ospB"
                     /locus_tag="OO609_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6634991.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5986..6726
                     /gene="phoN2"
                     /locus_tag="OO609_003746"
     CDS             5986..6726
                     /gene="phoN2"
                     /locus_tag="OO609_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6634992.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7086..>7188)
                     /locus_tag="OO609_003747"
     CDS             complement(7086..>7188)
                     /locus_tag="OO609_003747"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6634993.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2252 a         1356 c         1385 g         2195 t
ORIGIN      
        1 acaattaata tacattaagt aacaggcatt cgagccttga tttgagaaag cctgtcattc
       61 atttgtttta tgtagctatc tatttcctct gatgcataac taaaaagttc tcccatttca
      121 cgagcaataa taatctcaga tttaatagac tttaatctct ggcgactcat ctcttaacac
      181 ttgcataatc tctcttttta tatcatatag ttctataaac cgatttgccc gtagttctcc
      241 ttgggaagaa tcatttctga atacaacaat tatatacaaa aaatcactat tttctttaga
      301 gaagatctta caactaatct ctgggagctc acataatgca tgtaagtcag gatggtacaa
      361 actatccaca gaaaattgcc ccataggacg ttttccagat ataactgtca accagcttcc
      421 ttccggtgta ctggcttcgg ctctggaaat aggctgaaaa tcttccggac atggagaata
      481 cattgtcttc atataattaa gtcgtctgga tttctcatta ataaactcct ctatttttaa
      541 ttgtgccata cttctggtta taccagagat atacttcttg tcatatatgt ttggtgattc
      601 attaaagtaa ccatgttcaa ctctaggata aatatttctc actatatatt cagtatttgc
      661 agtatttatt ggaaatacgt tcttaatatt cttgatggga ctaatcattt taatctctat
      721 actcaaatat aaacattacc atgaacaata taattgttcc taagtggaat gtctccacgg
      781 agaacacttt atctatcggg aaatagtgcg ggcaacagtg tttaattata tcgagtgcaa
      841 ttacaatcgc tggcgacggc accgttggtg tggcggcctc agtccggaac aatttgaaaa
      901 ccagaacctc gcttaggcct gtgtccatat tacgtgggta ggctcacaga gcctcactta
      961 cgactgcgtc catattatgt gggtaggatc aataaagacg ttttatatgt gtctgcttgc
     1021 agccagaaaa tttataccct gcaggtatca taaacatgct tgataactta attggggctc
     1081 cctcttcctt ttatggtgta attgtttgat tgaatacttc cggggatttc agtatgaaat
     1141 gaagtatatt taatatactt tacaaaaagt ttgtattagt ggaataagga caatagctta
     1201 tccaaactgc caagcctctt tattataagt aagatctggc actggaatat tctcaaaagg
     1261 actaacatca tcaccacgac cttcaaccaa ccattttatt gcatttaaat cccagaatag
     1321 ttcagtagtg gtcaatttgg gaaacgcaca atattgtacg gttacgttat cgcaatagag
     1381 tgaactctcg ccctatacgg cgttttcaag gattagtcct taatcggaca accacattaa
     1441 agcataataa ataattaagc tttaataata tactatagat acagttctat tataaaaaat
     1501 actattttag gtgatgcatt aaattagtag aatatttatt cttttataaa agcaccaatg
     1561 aattgtccaa gtaaacatga gataaaatgt gtagaggata actgagtttg gtcactttaa
     1621 ctgtattagt cgcagtctga ctctcgactt taaaaggatg ggaattacct gtactgatag
     1681 tgagaaagct ttccattgaa ataagtcaaa taaaagttga tggcttcagt gctaaagtga
     1741 ggtgcccgct agccgtattc actcgaatcg gaattcaatg tattgcttct gccatttcca
     1801 taatatttca cgggaaaaac aaaatctgaa aaactatact taagtgttgt attgagtcag
     1861 ttgagataca aatacatatt tatcagcaca cgttacgtca aatacaggag gttgtcatgt
     1921 tggtactgaa aggcacgttg gctattgcag ccgtgatgac aatttattgt cttatcgttg
     1981 ttcttatgga acgcttttct gattgatttc atattggcga gagaacaaga aggtgagtag
     2041 aattgtcgcg gaatcctgaa actatcagcc tcaggtatga acaccaaaag gtaggtagag
     2101 aaatctatgg cccccgttac tttattcgca caatgaggta tttttttatt tttacaaaag
     2161 ttcaataaat attataataa aagatatgtt cttggtaaca taaacaaata tttaattaaa
     2221 ataatgcttg gcattgacgt ttatcttata aagtaagaag atcatggaca tttcaaccaa
     2281 aaaagtagag ttctcgatga aattaaaatt ctttgtactt gcactttgtg tacctgcgat
     2341 ctttactaca catgctacca ctaactatcc acttttcata ccggacaaca tcagtacgga
     2401 tattagtcta ggatctctga gtggcaaaac aaaagaacgc gtttatcatc ccaaggaagg
     2461 ggggagaaaa attagtcaac tggactggaa atacagtaat gcgactattg ttagaggtgg
     2521 catcgattgg aagctaattc caaaagtgtc tttcggagtt tccggttgga ctactttagg
     2581 taaccagaaa gcaagcatgg ttgataaaga ctggaacaat tccaataccc ctcaggtatg
     2641 gaccgaccag agctggcatc ccaatacgca tctccgtgat gctaacgaat tcgagctgaa
     2701 tcttaaaggt tggttattaa ataatttgga ttatcgactt ggactaatag caggttacca
     2761 agagagtcgt tacagtttta atgcaatggg agggagttat atttatagtg aaaacggggg
     2821 gagcaggaat aaaaaagggg cacatcctag tggtgaaaga acaataggtt acaaacagct
     2881 cttcaaaata ccttatattg gattaactgc taattaccgc catgagaatt ttgagtttgg
     2941 agcagaactg aaatatagtg gttgggttct ttcatctgat acagataaac actatcagac
     3001 tgagacaatt tttaaagatg aaataaaaaa ccaaaattac tgctctgttg ctgcgaatat
     3061 tggatactat gtcaccccca gtgcaaaatt ttatatagaa ggctccagaa attacatttc
     3121 taataaaaaa ggtgatacat ctctttatga gcaaagtacc aatatatctg gcaccattaa
     3181 aaatagtgca agtattgaat atattggttt tctcacttcc gcaggtataa agtatatttt
     3241 ttgaattggt tcaccgagtt gattacgttt tcatggctaa aaattgccgc ccattgtgag
     3301 tcatattcgc cctgactttc cagaaccata cgaactgccc gttgacggac ttcgggggaa
     3361 aaacgtgtat ttttagtcat tctgtttacc tctttctcag ggcgtttagt ctccaggatt
     3421 tccggggcgg ttcaaaaaac tcttcgttga ttactgtgga cttactgttg gcattaccga
     3481 ccctgagacc ggagaaataa gaaccgctta gatcatcgtg gctgttctcg gggtatcaag
     3541 ttacacatgg gcagaggcta cccaaggtct actattatcg cctgaaagcg ctgatggaac
     3601 agtgctatca gggacatgct gatgaaaata cctaaataat cagccagcac tctatctttc
     3661 caaatccaca gcatagcaaa gagagcaaaa gagcctgtaa attcagaaat taaggaaacg
     3721 cctcttggtg tacataatct atcttatgtt aaatgaaatc taatttcttg attatatgct
     3781 ggatcgttcg tgggtggctt tgcccctata tttccagaca cctgttatca ttggcccgct
     3841 gccgcagata ttcccgtggc gagcgataat ccagcgcact atgcggatgc cattcgttat
     3901 aatgctcgaa tgcctctgca aggttctttg ctgccgttaa cccgtcaggt ttgggcatga
     3961 tactgatgta gtcacacttt attgttttca cgaagctctt tgctattctg ttactctccg
     4021 gactccgcac cgccgtgttc ttcggttcaa caaagtccca acatccgggt gaactggcgt
     4081 gtttcattag ctcggtagca tgaaccatta tccgtcagtc acttcactgg agacgccgga
     4141 agctcgttgc cgaagcggtg ttccaccgct cccagcatgt cgccaacggg agagcatcta
     4201 cggtctgatt gaactgttta ttcaaattgt ccaccgtctg tcagtacgct ctgaacgtcg
     4261 gctggttaaa acactgctcg ctgattttca gagggttcac ggtaaaacag cacttttatt
     4321 ccggattgcg gaagcggctc tgaataatcc ggatgggctg gttaaagagg ttgtgtacca
     4381 tctgcatatt gttgaaccag atcgcagcgg taagagaagt tcatcatact tggcgcagtt
     4441 acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac cggaacaaga
     4501 tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct ccacggtact
     4561 ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt gaaattcttt
     4621 cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat ataattgcat
     4681 ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat atgaaaatat
     4741 aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt atgaatttag
     4801 atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata tcagatattg
     4861 catttgcaca tataataaaa agggtaaaaa attcatcatg tactcaccca aaagcagcat
     4921 tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct attatgggac
     4981 aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt aaaaatgaaa
     5041 aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt gaaccaatag
     5101 taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta aaaaaagcga
     5161 aaataaaaac agtaaacgat actgatttta aagagtataa taaggtttat attcttgggc
     5221 acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat gtacaaacaa
     5281 tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt tttacttcat
     5341 gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct gaaagtcttt
     5401 cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta gagcagataa
     5461 aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc ggctatcatg
     5521 gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt tcaacttcaa
     5581 ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact tttattatta
     5641 ataaagaact ggattagtat aaaattttta acctatagtt aattgtttgc atttaagatg
     5701 aagttatttt atctttagta gaaaagtgct tccctatgag ggaaaatcac aaatcatcat
     5761 aatacagaga caaaacgata cgaaaaaata gataaaaaac atcattcttt taccacatta
     5821 ttttccgtga atatgaaaaa taatgttatt actttaatat aagactattt tttgtttttc
     5881 catcactctg ttcaaatttt tccgcatgac ttgtgttttt tgtaatacag ctcgtttttt
     5941 acagctgacc aaaatcatca attaattatg ctaaggaaat aaattatgaa aaccaaaaac
     6001 tttcttcttt tttgtattgc tacaaatatg atttttatcc cctcagcaaa tgctctgaag
     6061 gcagaaggtt ttctcactca acaaacttca ccagacagtt tgtcaatact tccgccgcct
     6121 ccggcagaga attcagtagt atttcaggct gacaaagctc attatgaatt cggccgctcg
     6181 ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg catactacga gaattttggt
     6241 cttgcatttt cagatgctta tggcatggat atttcaaggg aaaatacccc aatcttatat
     6301 cagttgttaa cacaagtact acaggatagc catgattacg ccgtgcgtaa cgccaaagaa
     6361 tattataaaa gagttcgtcc attcgttatt tataaagacg caacctgtac acctgataaa
     6421 gatgagaaaa tggctatcac tggctcttat ccctctggtc atgcatcctt tggttgggca
     6481 gtagcactga tacttgcgga gattaatcct caacgtaaag cggaaatact tcgacgtgga
     6541 tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt ggcaaagcga tgtagaggct
     6601 gggcgtttaa tgggagcatc ggttgttgca gtacttcata atacacctga atttaccaaa
     6661 agccttagcg aagccaaaaa agagtttgaa gaattaaata ctcctaccaa tgaactgacc
     6721 ccataaagct ggacagcctg tatcaggcta tggagggccc atagacaaat ctaccctata
     6781 tgagcatagg aggagtctat gggcacacca cgttttaccc ctgaatttaa gggattactg
     6841 gaaaggctgg gacatatccc tccggcagaa gcagaaaaag cttattatgc tgccatcgga
     6901 aacgatgatc tggcaacctg agttcacaga taaaacattc tctaggaaac tcggggcggt
     6961 tccgttcacc acatgcaatg tggtgttgca ggggaacggt ctgcaccatc ccctatgtcg
     7021 atcaatataa cagaaatgac aacttcagat tcagggcaca acctaaatat attttaggtc
     7081 acctctcaaa tcgtttgcct gataccgctc cattttttaa taaaaaaatc aatcattttt
     7141 gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactattt
//
LOCUS       DAJTYH010000164         6849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTYH010000164 DAJTYH010000000
VERSION     DAJTYH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(57..959)
                     /locus_tag="OO609_003748"
     CDS             complement(57..959)
                     /locus_tag="OO609_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6634994.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1038..1991)
                     /gene="lsrR"
                     /locus_tag="OO609_003749"
     CDS             complement(1038..1991)
                     /gene="lsrR"
                     /locus_tag="OO609_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6634995.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2240..3775
                     /gene="lsrA"
                     /locus_tag="OO609_003750"
     CDS             2240..3775
                     /gene="lsrA"
                     /locus_tag="OO609_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6634996.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3769..4797
                     /gene="lsrC"
                     /locus_tag="OO609_003751"
     CDS             3769..4797
                     /gene="lsrC"
                     /locus_tag="OO609_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6634997.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4797..5789
                     /gene="lsrD"
                     /locus_tag="OO609_003752"
     CDS             4797..5789
                     /gene="lsrD"
                     /locus_tag="OO609_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6634998.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5801..6824
                     /gene="lsrB"
                     /locus_tag="OO609_003753"
                     /pseudo
     CDS             5801..6824
                     /gene="lsrB"
                     /locus_tag="OO609_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1570 a         1744 c         1806 g         1729 t
ORIGIN      
        1 agacatgtcc gcgctacagt ctcaccacag cgatcactgc tttcaaagga tgggactcat
       61 gacggcatac ggaacggagt aatcggcaac tgatgccggg cctcgctgag cgtgcactgc
      121 ttattatccg tgaacgtgcc gccaagaacc gcggtttgtg ccggacgcac aacgcctaac
      181 ccaaggcaac caagctgcac gtcgcctcct ccaacgacca ccagagtgcc cgccttcaga
      241 ccgcagagtt ccgccgcttg tgaacttacc acgcccagca atgtgccggt ttctttgaca
      301 ggagaaagaa tatcggcacg caggccagcc atatccagca atgcaggttt ccaggcacgg
      361 gtggttagat caagaagtcc cgtggtgcca gcgttagagg gatccaccgc cagttcgccg
      421 ctgagcatat aggccagcca gtcgctgatc atggtgatgg ttgatgcctg acggtaaata
      481 tcggaacgat ggtgcgccag ccaaagtaat ctggggatgg cacttaaagc cagtgtttgt
      541 ccggtcgctc gataaacttc gttttcaaag gtattgttgt gcagttcttt aagttcgcta
      601 acttcgcgtg ccgctctggc atccacattg gcgcaggccc agatcggtgt tccttcattg
      661 ttatataaaa caatgccttc acgcatagaa catgccgaaa cggcagcgat atactccggg
      721 tctatgccgg cgttgtgcag cgcctggcgc atacactcac acgccagttg ccagtttttg
      781 ttgagatcaa attccataga accaggaacg tccggtactg ccagatgccg ccactccgcc
      841 tgtcccactg ctatttgatt gccttccagg tcgaatatca cagcccgaat acttccggtg
      901 cctgcatcca gcgccatcag gtagtacttt gattctgaag gggtaaagag tcgagccatt
      961 agattatcct cggcttatgc gttctgtctg ttcctctata cgttctccat cattcccggt
     1021 aataaggtca tgcaaattta actacgtaaa atcgccgctg ctgtgtcctg atcggtaacc
     1081 agtgcgttga tataaccgcc tttcattgca gcggcaattg cttcagcttt attttctccc
     1141 cctgccacgc caacccggac gggtatggtc ttcagcgcgc ttaaaggtaa gccaatcagt
     1201 tcgttatgta ttttgatatc cgtgacaacg tcaccttttg catcaaaaaa gtagcctaaa
     1261 atgtcgccaa ccgccccttt tcggccaatc attaactgtt cgccctggct gatataaccg
     1321 gagcgaatga ttgtcgcgtc gtcttgttga ctcacggctc caatgccgac aatcgccaca
     1381 tccgctgctt gcgcggctag cagaacatct ttgacgcaat tttcattttt tagcgtacgg
     1441 gcaatgtcag cggaggatgc ccgcaacgga gccggaataa tattcacact gcacgccgcg
     1501 ttaagctgcc cgatacccgt catataagaa ccgacgccac cggagagcgt gaccaggcga
     1561 atttgctgtg acgaaataaa accacttaag cgttgcagcg tattcatggt tgcctcgcca
     1621 aaaccaatcg ccagcatctg ttgtggttga agtaaactca tcaacatatg cgccgcgcct
     1681 atccctagtc gcccgccgac atcagcatcc gcaagcccag ggatcacccg gacatgttgc
     1741 agcgaaaact gacgacgtaa ttgagtttca tattccagac agccttcaaa gcgagaatta
     1801 atctgtacgc gaataatgcc ggactgatgc cctttctcca gcaagcgcga cactttcaaa
     1861 cgggtcaggc cgagacgatc gctgatctcg ctctgggtca gcccgtcgtg atagtaaaac
     1921 cacgcgatcc gcgcgacctg ttcttcttca cacattccct gttctgaaat tgccgaatcg
     1981 ttgattgtca taattcattc ttcactttga acatatttaa atctttaatg caattgttca
     2041 gttcttgcgc atttatatct gtgatggcaa ccacagtttg actctacgag catgaacaaa
     2101 cgcaaccgtg aaaatcaaaa tagcataaat tgtgatctat tcgtcggaaa tatgtgcaat
     2161 gtccacctaa ggttatgaac aaattaaaag tagaaataca tttgttcaaa actcacctgc
     2221 aaaactgaac gggggaaata tgcaaacgag tgatacccgc gcgttaccgc tactttgcgc
     2281 ccgctcggtt tataaacagt attcaggggt caatgtcctg aaaggcatcg attttacgtt
     2341 gcatcagggg gaggtccacg ccctgctcgg cggcaatggt gccggtaaat cgacgttaat
     2401 gaagattatt gccggtatta cccctgctga tagcggtacg ctggagattg ggggcaacaa
     2461 ctacgccaga ttaacgccag ttcatgctca tcagctgggc atttatctcg ttccccagga
     2521 accgctgctt ttcccaagcc tgtcgataaa agaaaacatc ctgtttgggc tggcaaaaaa
     2581 acagctctcc atgcagaaaa tgaagaactt gctggcggcg ctgggctgcc agtttgatct
     2641 gcatagtctg gcaggatcgc tggatgtcgc cgatcgccaa atggtggaaa tcctccgcgg
     2701 gctgatgcgc gactcgcgga ttctgatcct cgatgaacct accgcctcgc ttacccctgc
     2761 ggaaaccgaa cgcttgttta gtcgcttgca agagctgctt gctactggcg tgggtattgt
     2821 ttttatctcg cataagctgc cggaaattcg ccagattgcc gatcgaatta gcgtgatgcg
     2881 cgacggaacc atcgccttaa gcggcaaaac cagcgaactg tctaccgacg acattattca
     2941 ggccatcacc ccagcggtac gggaaaaatc gctctctgcc agccaaaaat tatggctgga
     3001 gttacctggt aaccgcccac aacatgccgt cggaacgccg gtgctgacac tggaaaatct
     3061 gactggcgaa ggcttcagga atgtcagcct gacgctcaat gccggagaaa ttctgggcct
     3121 ggctgggctg gtgggagccg gacgcacaga actggccgag acgctctatg gtctgcgtac
     3181 tttgcgtggc ggacgcatta tgctgaatgg taaagagatc aataaattat ccactggaga
     3241 acgtttactg cgcggtctgg tttatctgcc ggaagatcgc cagtcatccg gactgaatct
     3301 cgatgcttcg ctggcctgga acgtctgcgc ccttactcat aaccttcgtg gattctgggc
     3361 gaaaaccgcg aaagataatg ccaccctgga acgttatcgt cgggcgctga atattaaatt
     3421 taaccaaccg gaacaagctg cacggacatt atccggtagc aaccagcaaa aaatcctcat
     3481 tgccaaatgc ctggaagctt cgccgcaagc attgattgtc gatgagccga cgcgcggcgt
     3541 ggatgtctcg gcccgtaatg atatctacca gctgttgcgc agcatcgccg cacagaatgt
     3601 ggctgtgctg tttatctcct ctgatatgga agagatcgaa ctgatggcag atcgtgtgta
     3661 tgtgatgcat cagggcgaaa ttacccactc tgcactgacc gggcgcgata ttaatgtcga
     3721 gactattatg cgcgttgcct tcggcgatag tcagcgtcag gaggcatcat gctgaagttt
     3781 attcagaaca accgtgaaat cacggcattg ctggcggtgg tgctgctgtt tgtattaccc
     3841 ggttttctcg accgccagta tttaagtgtg caaacgctga ccatggttta tagcagcgcg
     3901 caaatcctga tcctgctggc aatgggcgcg acgctggtaa tgcttacgcg caatattgat
     3961 gtttcagtgg gttcgattac cggaatgtgc gcggtgctgt tggggatgtt actgaacgca
     4021 ggatattcac tacctgttgc ctgtgtcgcg actttactgc ttggtttgct cgcgggattt
     4081 ttcaacggtg ccctggtcgc gtggctaaag atccctgcca ttgttgccac ccttggcacg
     4141 ttagggttgt acagaggcat catgttgctg tggactggcg gtaaatggat tgaagggtta
     4201 cccgccgaac tgaaacagct ctccgccccg ctgctgcttg gcatttcagc aattggttgg
     4261 ttgacgataa ttctggtggc atttatggcc tggctgctgg cgaagacggc gtttggacgc
     4321 agtttttatg ccacgggcga taatttacag ggcgctcgtc aactgggcgt tcgtacggaa
     4381 gccattcgca ttgtggcatt ttcgttgaac ggctgcatgg cggcactggc gggaattgtg
     4441 tttgcttcgc agattggttt tatcctcaac cagaccggta ccgggctgga gatgaaagca
     4501 attgcagcct gcgtgctggg cggcattagt ttgctcggtg gttccggtgc gatcatcggt
     4561 gcggtactcg gcgcatggtt cctgacgcag atcgatagcg tactggtgct gttgcgcatt
     4621 ccggcatggt ggaatgattt tatcgcgggt ctggttctgc tggcggtgct ggtgtttgat
     4681 ggacgcctgc gttgtgcgct ggaacgtaat ctacggcggc aaaaatatgc ccgctttatg
     4741 acgccaccgc catccgttaa acccgcttcg tcaggtaaaa aacgggaggc cgcataatgc
     4801 gtattcgcta cggttgggaa ctggctcttg ccgcactgct cgttattgag attgtcgcat
     4861 ttggtgcaat taacccgcga atgttagatc tcaatatgtt gctgttcagc accagtgact
     4921 ttatctgcat tggcattgtc gctctaccgc tgacgatggt gattgtcagt ggcgggatcg
     4981 atatttcgtt tggttcgacc atcggcctct gcgccattgc attgggcgta ctgtttcaaa
     5041 gtggtgtgcc gatgccgctg gcgatactcc tgaccttact gctcggcgca ttgtgcgggc
     5101 tgatcaacgc cggattaatt atctatacca aagttaaccc gctggtgatt acgcttggca
     5161 cgctgtatct gtttgccgga agcgctctgc tgctttccgg tatggccgga gcgacggggt
     5221 acgaaggtat tggtggattc ccgatggcgt ttacagattt cgctaacctg gatgtgctgg
     5281 gactccccgt tccgctgatt atcttcctga tatgtctcct cgttttctgg ctctggctgc
     5341 ataaaaccca tgccggacgt aatgtgtttt tgattgggca aagcccgcgc gtggcgcttt
     5401 atagcgcgat tccagttaac cgcaccttat gtgcgctcta tgccatgacg gggctggcgt
     5461 ctgcggtcgc cgctgtgctg ctggtatcgt attttggttc agcacgttcc gatctcggtg
     5521 cgtcgtttct gatgcccgcc atcaccgccg tggtgcttgg cggggccaat atttatggtg
     5581 gttccggttc cattatcggc accgccattg cggttttatt agtgggatat ttgcagcaag
     5641 gtttgcaaat ggcaggagtg ccaaatcagg tgtccagcgc cctttccggt gcgctgctta
     5701 tcgtcgttgt cgtaggtcgt tccgttagcc tgcatcgcca gcaaattaaa gagtggctgg
     5761 cgcgtcgggc caataaccca ttgccataaa ggatatcttc atgacacttc atcgctttaa
     5821 gaaaatcgcc ttacttagcg ctcttggcat tgccgcaatc tctatgaatg tgcaggccgc
     5881 agagcgtatt gcatttattc ccaaactggt tggcgtggga ttttttacca gcggtggcaa
     5941 cggcgcacaa caagcgggta aagagctgag cgttgatgtg acctacgacg ggccgacaga
     6001 acccagtgtt tctggtcagg tacagttgat taataacttc gtcaatcaag gttataacgc
     6061 cattattcgt ttctgcggtt tcgcctgatg gcttgtgtcc ggcactgaaa cgcgccatgc
     6121 aacgtggtgt gagagtgctg acctgggact ctgatactaa accggagtgc cgctcttact
     6181 acattaatca gggaacgccc gcccagttgg gaggtatgtt ggtggatatg gcggcgcgtc
     6241 aggtgaataa agacaaagcc aaagtcgcgt ttttctactc aagccccacc gttacggacc
     6301 aaaaccagtg ggtgaaagaa gcgaaagcga aaatcgccaa agagcatcca ggctgggaaa
     6361 ttgtcactac gcagtttggc tataacgatg ccactaaatc gttacaaacc gcagaaggaa
     6421 tattaaaagc gtatagcgat ctcgacgcca ttatcgcccc cgatgccaac gccctgcccg
     6481 ctgccgcaca agccgcagaa aacctgaaaa atgacaaagt agcgattgtc ggattcagta
     6541 cgccaaatgt gatgcgcccg tatgtagagc gcggcacggt gaaagaattt ggactgtggg
     6601 atgtggttca gcaaggcaaa atttcagtgt atgtcgcgga tgtattattg aaaaaaggat
     6661 caatgaaaac gggcgacaag ctggatatcc agggcgtagg tcaggttgaa gtctcgccaa
     6721 acagcgttca gggctatgac tacgaagcgg atggtaatgg catcgtactg ttaccggagc
     6781 gcgtgatatt caacaaagag aatatcggca aatacgattt ctgatgtgca ttacttaacc
     6841 ggagtaagt
//
LOCUS       DAJTYH010000165         6661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTYH010000165 DAJTYH010000000
VERSION     DAJTYH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            58..1482
                     /gene="sbcB"
                     /locus_tag="OO609_003754"
     CDS             58..1482
                     /gene="sbcB"
                     /locus_tag="OO609_003754"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6634999.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1525..1752)
                     /locus_tag="OO609_003755"
     CDS             complement(1525..1752)
                     /locus_tag="OO609_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6635000.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1766..2824)
                     /gene="yeeE"
                     /locus_tag="OO609_003756"
     CDS             complement(1766..2824)
                     /gene="yeeE"
                     /locus_tag="OO609_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6635001.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3003..4361)
                     /gene="plaP"
                     /locus_tag="OO609_003757"
     CDS             complement(3003..4361)
                     /gene="plaP"
                     /locus_tag="OO609_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6635002.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4351..4413)
                     /gene="yoeI"
                     /locus_tag="OO609_003758"
     CDS             complement(4351..4413)
                     /gene="yoeI"
                     /locus_tag="OO609_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6635003.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4574..5557)
                     /locus_tag="OO609_003759"
     CDS             complement(4574..5557)
                     /locus_tag="OO609_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6635004.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5603..6427)
                     /locus_tag="OO609_003760"
     CDS             complement(5603..6427)
                     /locus_tag="OO609_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6635005.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6510..>6661)
                     /gene="yoeB"
                     /locus_tag="OO609_003761"
     CDS             complement(6510..>6661)
                     /gene="yoeB"
                     /locus_tag="OO609_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6635006.1"
                     /translation="RTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAA
                     CRYHY"
BASE COUNT         1799 a         1753 c         1571 g         1538 t
ORIGIN      
        1 ggttgctttt cgagttataa cagacagtta attaactaca gtggacaatg ctagacaatg
       61 acagacacag ataaacaacc taccttcctc tttcacgatt acgaaacctt tggcacgcac
      121 cccgcgttag atcgccctgc acagttcgca gccattcgca ccgatgacga attcaatgtc
      181 atcggcgaac ccgaagtctt ttactgcaag cccgcggatg actatttacc ccggcctgga
      241 gcagtattaa ttaccggtat taccccgcag gaggcacggg cgaaaggaga aaacgaagcc
      301 gcgtttgccg cccgtattca ctcgcttttt accgtaccga agacctgtat tctgggctac
      361 aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt tttatcgtaa tttctacgat
      421 ccttacgcct ggagctggca gcatgataac tcgcgctggg atttactgga tgttatgcgt
      481 gcctgttatg ccctgcgccc ggaaggaata aactggcctg aaaatgatga cggtctaccg
      541 agctttcgcc ttgagcattt aaccaaagcg aatggtattg aacatagcaa cgcccacgat
      601 gcgatggctg atgtgtacgc cactattgcg atggcgaaac tggtaaaaac gcgtcagcca
      661 cgcctgtttg attatctctt tacccatcgt aataaacaca aactgatggc gttgattgat
      721 gttccgcaga tgaaacccct ggctcacgtt tccggaatgt ttggggcatg gcgtggcaat
      781 accagctggg tggcaccgct ggcgtggcat cctgaaaatc gcaatgccgt aattatggcg
      841 gatttggcag gagacatttc gccattactg gagctggata tcgacacatt gcgcgagcgt
      901 ttatataccg caaaagccga tcttggcgat aacgccgccg ttccggttaa gctggtgcat
      961 atcaataaat gtccggtact ggcccaggcg aatacgctac gcccggaaga tgccgaccga
     1021 ctgggaatta atcgtcagca ttgcctcgat aacctaaaaa ttctgcgtga aaatccgcaa
     1081 gtgcgtgaaa aagtggtagc gatattcgcg gaagccgaac cgtttacacc ttcagataat
     1141 gtggatgcac agctttataa tggctttttc agtgacgccg atcgtgcagc aatgaaaatt
     1201 gtgctggaaa ccgagccgcg taatttaccg gcactggata tcacttttgt tgataaacga
     1261 attgaaaagt tgttgttcaa ttatcgggca cgcaacttcc cggggacgct ggattatgcc
     1321 gagcagcaac gctggctgga gcaccgccac caggtcttca cgccagagtt tttgcagggt
     1381 tatgctgatg aattgcagat gctggtacaa caatatgccg atgacaaaga gaaagtggcg
     1441 ctgttaaaag cactttggca gtacgcggaa gagatcgtct aatttgaagc cgccgccgct
     1501 ggtacggcgg aggacaatgt tgagtcaggc tttttgaacg gtgatgctcc acgctgcatc
     1561 gccaatctgc tgataatcgg tgatggcatg tccttcttct gccgcccact gtgggattgc
     1621 ttcagttgcc tgggtgcaat caaactcaat taccaactca tcgccgctgg ccatttctgc
     1681 cagtgctgct tttgcttcta tgagtggaaa cggacagacc tgcgtcacca catccagctt
     1741 tttaatcgcc atctttacac cttacttaat ttgccgcagc agttgccagt cgcgccttac
     1801 gctgcggtcg aacatacaca agccaggacg cagtccagac tccgagaatc ataaatacaa
     1861 ggccaatcca gccctgccag gtcatcattg cagtcataac cagtccatta ccgatagagc
     1921 aaccacctgc aatactggca ccgaagccca tcagtacacc acctaacccg ctacgtaatg
     1981 ttgtttgtgc atcagctgcg cgaacgcgga actcacggct cgctttggct gcaataaatg
     2041 cctccacgaa gatccctaac accaggaaaa cgccccagtt aatgtatttc acgtcacccg
     2101 cgaccagaaa ttgaagaata ttggccgttg gggaagtgac tcctaaccca aacatgcgtc
     2161 cggttgcttc actcaggggc caggctaaaa gcgcaatcaa accgatgagt acagcagtga
     2221 caaagggatg ccagcgtttt tcaaacagaa tatgagcgat cccggttcgg cgcggcggta
     2281 aggtcgcgac tttgagtttt ggcttcttca actctttcat caccacccag agcgttatca
     2341 ccagcaaaac ggtaaccaac ggccacacag acaaattaaa agtctcagca atagagttat
     2401 gttcagtagt gtagtgctgc aaggtttgat ttaaaccact ggcgtgtgga gaacgcatca
     2461 ccgcactcat caccatataa gtgaaaagcg cgatccaact gccgatcaat ccctcacccg
     2521 cgcgatacca ggtcccggtg gcacatccac ccgccagaac aattcccagc ccgaaaagat
     2581 acccacctat aacagtacca agccacggga acgccccggc ttcgtaagtc agtaggcccg
     2641 cctgaattaa cgcaaaaacc cccacgcttt gtgccgaaat tgcaatcagc aaggcgtaaa
     2701 acatgcgatt atttttcgcg atatacatat cgcgaaaacc accagtcagg caaaaacgcc
     2761 cacgctgcat gacaaatccc agcagagcac cacaaattag cccgcttaat atcattgaaa
     2821 acataaatta attaaccaga tgaatgttaa tggggaaatt tttcatgact ggtggaatgc
     2881 agaccaataa ccaaattctc taaattagaa caaatggtta ttaatgagga tttcgataaa
     2941 aaagaataaa aaaaccggag ccaaatgttc attcgactcc ggtttattat tagaaaagat
     3001 gattatgcta cgtcttcgta ctgcggaacc ggattacgga agctcttggt gacgcaagcc
     3061 aggtaaatca gaccgatagc agcccagatc agacccagaa ccattgagct ttcttccagg
     3121 ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag atactggaag
     3181 tgatctttca gcgtcttgtt acgcttctca cggatccaga actgcgagat gaccgacagg
     3241 ttaacaaagg tgaacgccac cagcgcacca aagttaatca gcgccgttgc cattaccagg
     3301 tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat cgccggagta
     3361 cgccatttcg ggtgtacata accgaagaag cttttcggga acacgccgtc acgacccatt
     3421 acgtacatca gacgcgctac gcctgcgtgc gccgccatac cggacgccag tacggtaatg
     3481 gtggagaaga tcagcgcgcc aacctggaac gctttacccg caacgtacag catgatttca
     3541 ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag ctgcaggaag
     3601 taagttgcaa agatgaagat catgccgcca atcagcgcgg tcaggaaaat cgcacgcggg
     3661 atcacgcgct ctgcatcttt ggtttcttcc gacagattgc tgatgccgtc aaagccggta
     3721 aaggagaagc acaggattgt cgccccggta atcatcggga taacatgcgc atcgccagac
     3781 cagaacggac gcgtgctcgc cagcgtacca gcgccttcac cttcaaatac gccataaaca
     3841 accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac agtgttgacg
     3901 ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac cagcgccacc
     3961 acaaacatcc acgatggaat ggaaggcacc agagcttcaa aatagatttt cgccagcaga
     4021 atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac cataaagccg
     4081 acagtcgggc taatggattt ctgggcgtaa gtgtatgcag agccagcaga aggatagcgg
     4141 cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc gaacgcatag
     4201 gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaatgtatc aaacagcgtc
     4261 atcggctgca tataggctag acccatcatt acaaccggaa ctaacgtaag cgttttacgc
     4321 aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggttattct cctttacggt
     4381 gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag cgacaacaac
     4441 tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta tgaatgattg
     4501 gtttgaagca aaaaataacc gacgctaatt aaacgtcggt ttttaatcat tttttgacag
     4561 cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaggctt gtcataataa
     4621 tttttctttg aatggctgat tttccgtcat cagattttgc ataaacaccg gcgatcgcac
     4681 tatttgctaa aatttcatct cgccaccagc cgctggcaag ccctgcggtc tgttcattcc
     4741 agccaatcca gacaggttca aagagagttt gggcgaccac tttcttctcg attaacgcgc
     4801 cactatcgag aaaacgttgc gccagatacc gtggtaaata gccgcagcca aggccgctaa
     4861 tttgcagctc cagtttggtt ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa
     4921 gttgtgaggc cgtagaagcg cctgcctgcg cagtatcccc caccacaatc gcccgatatc
     4981 gtttaatgat acgacggttt aacggttctt cttcctgggc taacggatga tgaggcgcga
     5041 ccgcaaagac ttgttcaaga tcgcccaacc gcgagaaacc aaactcactg gaagatggtg
     5101 gctcatgcat cgcgccaacg ataatatccg ctcgcccctg agtcagggca tcccaggaac
     5161 caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga taaaaagctt
     5221 caatgagagg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc accagctcgt
     5281 tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa
     5341 cttcccgccc tttctctaat aacatttttc cggtgcgggt gaatttagcg cggtgtccgc
     5401 tacgatcaag caattgaata tttaggtcgc tttccagctt gtgaacggtg taactcagag
     5461 cggacggcgt cttgtagagt ttggctgacg ccgccgcaaa actgccctct ttttctatgg
     5521 catcgaggat catcaacaca tccagcagtg gtttcataca cgcccccttg cagtgcatgc
     5581 ggcgtcctgc tggcgtgatc gtttactcca acggcattac cagcggatcg ggatactggt
     5641 attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct ttgccgctgt
     5701 ccagactatt tctgaactgc ggaggttgca gccccagtaa acgggccatc tgcggataga
     5761 aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg cctttaggtg
     5821 cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa ttaacaccat
     5881 gttcaccatc aggggcggtt tttccggcaa agaagcgtcc cggatgacgt cccggtccga
     5941 ccaggcccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc agccagtctt
     6001 cgagttcttc taatactcgt ccactgttgg ttaccggatt acgcggggta gtttctttaa
     6061 ccgtgccttg cgcgtcgcca tagacagatg ttgagctggt aaaaataatt cggggaatac
     6121 gatgggccag cgcgctatcc actaactctt gtaccgcttg taaatagaac tcatcgccgg
     6181 ggccgctacg acgtgccgga agcgtaatga ccagcgcatc ggcatccata agggcatcca
     6241 gatcgtcaga atcgcaaact aactcaggct ccatgcgaag cagatagcta tcaatgccac
     6301 tcattcgggc cgcttcgaca ccatcttgtg tggttttact cccggtgact tgccagcctc
     6361 gcgctgaaag tgacatcgcc agcggcatac ctaaccaccc taacccgaca attgcgacct
     6421 ttttcatccg ttatctcctg atacacctta gatctataag gctacgctag cgtatcaaaa
     6481 ctgacaattc attctatgaa tgaatctgtt caataatgat aacgacatgc tgcaatgagc
     6541 agtgaatcat cagtaaccgc gtataccaga cggtgctcct ctgtaatgcg tcgggaccag
     6601 aaacctgaca aattatgttt caggggttct ggtttcccct taccttcaaa tggtgttctg
     6661 c
//
LOCUS       DAJTYH010000166         6613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTYH010000166 DAJTYH010000000
VERSION     DAJTYH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            53..1543
                     /locus_tag="OO609_003762"
     CDS             53..1543
                     /locus_tag="OO609_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6635007.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1595..2752
                     /locus_tag="OO609_003763"
     CDS             1595..2752
                     /locus_tag="OO609_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6635008.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2718..2840)
                     /locus_tag="OO609_003764"
     CDS             complement(2718..2840)
                     /locus_tag="OO609_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635009.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3043..4728
                     /locus_tag="OO609_003765"
                     /pseudo
     CDS             3043..4728
                     /locus_tag="OO609_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4766..>6613
                     /locus_tag="OO609_003766"
     CDS             4766..>6613
                     /locus_tag="OO609_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6635010.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVD"
BASE COUNT         1948 a         1445 c         1392 g         1828 t
ORIGIN      
        1 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga
       61 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      121 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa
      181 atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta acggctatgt
      241 ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
      301 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
      361 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
      421 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
      481 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
      541 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
      601 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
      661 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
      721 accaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac
      781 agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
      841 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
      901 tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac
      961 ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg
     1021 taattatgac aagctgattt ccgcaatgga tttctaccca acagctcttg atgcagccga
     1081 tatcagcatt ccaaaagacc ttaagctgga tggtgttccc ttgctgccct ggttgcaaga
     1141 taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt
     1201 tgatgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga
     1261 cgattacccg cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa
     1321 taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac
     1381 ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca
     1441 aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgagg taaatcagga
     1501 gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc
     1561 ggatttttcc gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag
     1621 ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac
     1681 tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat atatcgaagc
     1741 gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga
     1801 ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa
     1861 tacattacaa acgaatggca ttttattgaa taatgaatgg tgtgcctttc tcaaagaaca
     1921 tgaatttctg gttggtatct cgatcgatgg cccccaggag ttacatgacc gttacagacg
     1981 cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc
     2041 atatcaagta gagtttaata cgttaaccgt cattaataac gttaatgtcc attatcctct
     2101 tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct
     2161 agaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat
     2221 tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg
     2281 ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt
     2341 tttggggaat gggcatacaa gttgtatttt ccaggagtcc tgcaaggata atctggttgt
     2401 tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg
     2461 aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa
     2521 acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc
     2581 taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa
     2641 aatccttttt tcaaccatgg taccttatat gaacgccatg gttgagttag ctaagaacag
     2701 agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc
     2761 tcttttaact ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt
     2821 cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg
     2881 cgtcagggca ttagcaatac ccttgattat caatagcata accataacca ctgaaaatta
     2941 aaaatttcta tatatttcat attgttaaat gatgcttaaa taaatttcat cagtaaatca
     3001 ttgccataga taatcattgg atggcacaat caattttcgc taatgataac cattcccatt
     3061 acgttttgta tgttgatagc aaagtatctc tgtctgctga agcccttttg gctgcgtaag
     3121 aataataaaa cttcagtgtt gttaatcatt attatcctcg caatgatcct tggcgtggtt
     3181 aaaattcagg tctggttaaa cgactggaat aatgattttt tcaatgcgct aagccagaaa
     3241 gaaaccgata agctttggca gctcgttctt tggttccctg ccctgctggg gatctttgta
     3301 ttaatatccg caaacaaaac atggttaatt aaactactga ctattcgctg gcgcgaatgg
     3361 ttaacagatt actaccttaa tcgctggttt gcagataaaa actactactt cacgcagatc
     3421 tacggcgagc ataaaaatac agataaccct gaccaacgta tcgctgaaga cattctctta
     3481 ctgatcaaca aaacgctcag tctttcattt ggcttcatcc agtcacttag catgctgatc
     3541 acctttaccg ttattctttg gcaaagcgcg ggtacgctct cattcactgt tggtggaaca
     3601 gaatggaata tccaggggta tatggtctat accgtcgtgc tcattgtgat cggtggaact
     3661 ttatttaccc ataaggtggg taaacgtatt cgtccgctta atgtggaaaa acaacgtagc
     3721 gaagcgacat ttcgaactaa tcttgtgcag cataataagc aggcagaact gattgcgcta
     3781 agcaatgctg agtctctaca acgccaggag ttgcgcgaca attttcatac cattaaagag
     3841 aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt actggcaaaa tatttattcg
     3901 cgctcgctta gcgttcttcc ctactttctg ttattgccgc agtttattag cgggcagatc
     3961 aatctgggcg gactgatgaa atcgcgccag gcatttatgc tagtatcgaa caatttaagc
     4021 tggtttattt ataaatatga cgaacttgct gaactggctg cggttatcga tcgcttgtat
     4081 gagttccatc aactcactga atagcgccct acgaataagc ctaaaaattg ccaacatgcg
     4141 gtacaagtgg ctaatgcgag tattcgtacg cctgataata agatcatatt agagaacttg
     4201 aactttcatg tttcgccagg caaatggcta ttactgaaag gctactctgg tgcgggaaaa
     4261 accacactgc ttaaaacatt atcccactgc tggccgtggt ttaaaggtga tatttcttct
     4321 cctgctgaca gttggtatgt gtcacaaaca ccgttaatca aaagcggctt actgaaagag
     4381 attatttgta aagcacttcc cctgcccgta gacgataaat cgttgagcga agtactgcat
     4441 caggttggtc tgggaaaatt ggctgcgcgt attcatgacc acgatcgctg gggagatatt
     4501 ctttccagcg gcgaaaaaca acgtatcgcc ctggcacgat taattttacg acgtccgaaa
     4561 tggatatttc ttgacgaaac tacctctcat cttgaggaac aagaggctat acgcttactg
     4621 cgtttagtgc gtgaaaaact acccacaagc ggcgtcatta tggttacaca tcaacccggt
     4681 gtctggaacc tggctgatga tatttgtgac attagcacgg ttttataaca acaataaaag
     4741 ccctgctgta cggagatata aataaatgaa gcgagttctt attcctggcg tcattttatg
     4801 tggcgctgat gtggcgcagg ccgtcgatga caaaaacatg tacatgtatt tttttgaaga
     4861 gatgacggtc tatgctcctg tccctgtacc cgtaaacggc aacacgcatt acaccagtga
     4921 aagcatcgag cgtttaccga ccgggaatgg caatatcagc gatctgctga gaaccaaccc
     4981 tgcggtacgc atggattcaa cgcaaagtac ctcgttgaac cagggagata ttcgccctga
     5041 gaaaatctct attcacggtg cgtcgcccta ccagaatgcc tatttgattg acggtattag
     5101 tgcaactaat aacctgaacc cagcgaatga gtccgatgcc agtagtgcaa ccaatattag
     5161 cgggatgtca caggggtatt atcttgatgt cagcttactg gacaatgtga cgctttatga
     5221 cagttttgtg ccggttgaat ttggtcgctt caatggcggg gtaattgatg caaagatcaa
     5281 acgcttcaac gctgatgata gcaaggtgaa attgggttat cgcactacgc gtttggactg
     5341 gttaacatcg catatcgatg agaataacaa gagcgcattt aatcaaggtt cttcaggaag
     5401 tacctatttc tctccagatt ttaaaaagaa cttttatacc ttgtcgttta atcaggagct
     5461 ggctgataac tttggcgtta ccgccggttt atcgcgccgc cagtctgata tcacccgcgc
     5521 ggattatgtt tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa acgttatcga
     5581 tactgcattg agcaaattta cctggtttgc cagcgaccgc tttacccacg atttaacctt
     5641 aaaatatacc ggctccagcc gtgattataa taccagcacc ttcccgcagt ctgatcgcga
     5701 aatgggtaat aaatcctatg gtctggcatg ggatatggat acgcaactcg catgggccaa
     5761 actacgtacc accgttggtt gggatcatat tagtgattat acccgtcacg atcatgacat
     5821 ctggtacacc gaactttcat gtacatatgg tgatattaca gggcgttgta cccgtggcgg
     5881 attaggacac atttcccagg ctgtagataa ttacaccttc aaaacacgcc tggactggca
     5941 aaaattcgcc gtgggtgatg tttcgcatca accctacttc ggcgcggaat acatctattc
     6001 cgatgcatgg actgaacgcc ataaccagtc tgaatcctat gtgattaatg ctgcaggaaa
     6061 gaaaactaac cataccattt accataaagg taaaggcagc ctgggaattg acaactacac
     6121 gctgtatatg gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg gtgtgcgtta
     6181 tgactatgac aactatctgt caaaccacaa tatctccccg cgctttatga cggaatggga
     6241 tatttttgct gatcaaacct caatgattac cgccggttat aaccgttact atggcgggaa
     6301 tattcttgat atgggattac gtgatatccg caatagctgg acggaatcgg tatcaggtaa
     6361 taaaaccctg acgcgttatc agaatttgaa aacgccttat aacgatgaac tggcaatggg
     6421 attgcagcaa aaaatcgata agaacgttat tgcacgcgca agtgaagcgc atgatcaaat
     6481 cagcaaaagc agtcgtaccg acagcgcgac taaaaccacc attactgaat ataacaacga
     6541 tggcaaaacc aaaacgcatt cgtttaacct cagttttgaa ctggccgaac ccctgcatat
     6601 ccgccaggta gat
//
LOCUS       DAJTYH010000167         6429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTYH010000167 DAJTYH010000000
VERSION     DAJTYH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..678
                     /locus_tag="OO609_003767"
     CDS             <1..678
                     /locus_tag="OO609_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6635011.1"
                     /translation="GRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNL
                     RNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSI
                     DILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPE
                     LEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAYKASDERLYVNK
                     QNKNSRS"
     gene            721..1224
                     /gene="yeaK"
                     /locus_tag="OO609_003768"
     CDS             721..1224
                     /gene="yeaK"
                     /locus_tag="OO609_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6635012.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1225..1329)
                     /locus_tag="OO609_003769"
     CDS             complement(1225..1329)
                     /locus_tag="OO609_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635013.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1499..1945
                     /locus_tag="OO609_003770"
     CDS             1499..1945
                     /locus_tag="OO609_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6635014.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1902..2720)
                     /gene="nimR"
                     /locus_tag="OO609_003771"
                     /pseudo
     CDS             complement(1902..2720)
                     /gene="nimR"
                     /locus_tag="OO609_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            2817..3997
                     /gene="nimT"
                     /locus_tag="OO609_003772"
                     /pseudo
     CDS             2817..3997
                     /gene="nimT"
                     /locus_tag="OO609_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4052..4399
                     /locus_tag="OO609_003773"
     CDS             4052..4399
                     /locus_tag="OO609_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6635015.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4421..4675)
                     /gene="yoaF"
                     /locus_tag="OO609_003774"
     CDS             complement(4421..4675)
                     /gene="yoaF"
                     /locus_tag="OO609_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6635016.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4858..5883
                     /gene="dgcP"
                     /locus_tag="OO609_003775"
     CDS             4858..5883
                     /gene="dgcP"
                     /locus_tag="OO609_003775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6635017.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            5916..6014
                     /locus_tag="OO609_003776"
     CDS             5916..6014
                     /locus_tag="OO609_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6635018.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6017..6091
                     /gene="yoaJ"
                     /locus_tag="OO609_003777"
     CDS             6017..6091
                     /gene="yoaJ"
                     /locus_tag="OO609_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6635019.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6252..6429)
                     /locus_tag="OO609_003778"
                     /pseudo
     CDS             complement(6252..6429)
                     /locus_tag="OO609_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1535 a         1570 c         1576 g         1748 t
ORIGIN      
        1 gggcgtaccg tattgtcgag catttaccag gatcagcgta ctaaacagaa cgtgatgagt
       61 ttgctgacgc cggtatatgt cgcagggcag ctaaaaggga ttgtgctgct ggatattaac
      121 aaaaacaatt tgcggaatat cttttacact catgaccgcc ctctcctctg gcgttttctc
      181 aatgtcacgc taaccgatac cgattcgggg cgcgacatta tcatcaacca gagcgaagat
      241 aatctgttcc agtatgtcag ttacgtccat gacttacctg gcggcattcg tgtctcgtta
      301 tccattgata ttctttactt tatcacgtct tcgaggaaaa gcgttctgtt ctggattttg
      361 acggcgttaa ttttgctgaa tatggtccgg atgcacttcc gtttatacca aaatgtgtcg
      421 cgagaaaata ttagtgatgc gatgactgga ctgtataatc gcaaaatttt aacccctgaa
      481 ctggagcagc ggttgcagaa actggtgcaa tccggttctt cggtgatgtt tattgcaatt
      541 gacatggaca agttaaagca aataaatgac accctcggtc atcaggaggg ggatttagcg
      601 attacgttac atgatgcgta taaagcttcc gatgagcgtt tatatgtcaa taagcagaac
      661 aaaaacagcc gttcatgata accttctgtg gttgtttgct tgtaatctca ggagcgtgaa
      721 atgactgaaa tggctaaagg aagcgtgacg catcagcagt taatcgcttt attatcacaa
      781 gaaggtgcta acttccgcgt cgttacccat gaagcggtag gtaaatgtga agcggtgtca
      841 gaaattcgtg gtaccgctct aggccagggt gctaaggcac tggtatgtaa agtcaaaggc
      901 aatggcgtaa atcaacatgt tctggcgatc ctcgcagccg atcaacaggc cgatctgagc
      961 caactcgcca gccatatagg cggattacgc gcttcgttgg ccagcccggc ggaagttgat
     1021 gaactcacag gctgtgtctt cggcgcgatc ccccctttca gcttccatcc aaaactcaaa
     1081 ctggttgccg accctctact cttcgaacga tttgatgaaa tcgccttcaa tgcagggatg
     1141 ctggataaat ccgttatttt gaaaaccgcg gattatttgc gcattgcgca accagaactg
     1201 gtcaatttcc gccgcactgc gtagctagcc tatccgttca ataagcagaa cggacaaaac
     1261 cagcacaacc gcaatagcaa aaaacgatga tgtgataatc agtgtctcga cgaacatttg
     1321 atcgttcata gcatgccccc gggagatagc tgttttcacg ttattgttag cgtgcacaaa
     1381 tggcagtttg atgacagttc gcctgatttt ttatgcaaaa aacgtaaaga ttttctactt
     1441 ccttcctgca gcaagcataa agtaagcagg cttattattt tttggcaagg aaaccacgat
     1501 gtttgatgtc actttgctga tcctgctcgg gttagctgcg ctgggcttta tcagtcataa
     1561 caccactgtc gccgtttcaa ttctggtgtt gatcattgtc cgcgtcacac cgttaagcac
     1621 ctttttcccg tggattgaaa aacaggggct aagtatcggg ataatcattc tgacgattgg
     1681 cgttatggcc cccatcgcca gcgggacgct accgccctca accttgattc actcgtttct
     1741 gaactggaaa tcgctggtgg caattgcagt aggcgttatt gtctcctggc tgggtgggcg
     1801 cggcgtgacg ttaatgggca gccagccgca actggttgct gggctgctgg tgggcactgt
     1861 tttaggcgta gcgctgtttc gcggcgtacc ggttggcccg cttattgccg cgggtctggt
     1921 ttcgctgatt gtggggaaac agtagttaat cccgcaatat atcggcctgg cgtttgcccc
     1981 aagcctttct taaacatagt aataaaggcc gttgttgaat cataccccag cgtatgagca
     2041 actttctgta cagtatcgcc tttcactaat ccctgaagcg ccataatcag ctgtaactgc
     2101 tgacgccact ggcgaaaact caaccccgtc tctttcacta tcaggcgggc aagattgcgt
     2161 tcactcatcg caaaaaagcc agcccattgc cccaatgccc cccactcgac gggccctttc
     2221 gccatcatct ccaccatcgt gcggatttta ggatgagaag aaacgggcag gtgcagctgt
     2281 tgttgtggct gctgagggag ttcatcaaac aggacctgaa ttaatctgcg ggtcatcggc
     2341 tcagcacgct gagtcgtcgt cctgtttgcc agggttaaaa tcaattcccg gcataagggc
     2401 gatattttca atgtgcaaca tggtcggcat tgtcactgcg ctgggttcga taaacagaaa
     2461 acagagttca gcgttagctg tcacttgatt gctatgcttc accccgcccg gtatccaaac
     2521 cgcatattgt ggcggcacca tccacaaagc attttccacc gtacaggtaa ttgcaccatg
     2581 taatgccagt atcagctgtc ctttgcggtg ctgatgtaca ggactggtta actcatccgt
     2641 tccggcgtga atacaaaatg cgaccgcggc ttcatggtgt cggtgggttc atagccattg
     2701 agattcaacc tgtgcatcat tttgtccgaa cttagcgata atttgtcatt ttagcttgat
     2761 tcaacataac aataaaaacg gtaaggtaca gcctcgtttg taacaatgag aagcatatga
     2821 cctgttcaac ttcattaagc ggcaaaaaca ggattgtcct tatcgctggc attctgatga
     2881 ttgccacaac attacgcgtc acctttaccg gcgcagcgcc gttactggat acgattcgtt
     2941 ccgcttactc gctgacgaca gcgcaaaccg gcttattaac caccctgcca ttattggcct
     3001 ttgcgctaat ctcacctctg gctgccccgg tggcgcgacg ttttggtatg gaacgtagcc
     3061 tgtttgccgc gttacttttg atctgtgcag gtatcgcaat tcgttctctc ccttcgcctt
     3121 acttattatt tggcggtaca gcggtcattg gcggtgggat tgcattaggc aatgtcttac
     3181 tgccaggatt aattaaacgc gatttccctc attccgtcgc cagacttacc ggcgcatatt
     3241 ccctgacaat gggagctgca gcggcactgg gatcggctat ggtcgtgccg ctggctttga
     3301 acggttttgg ctggcaaggc gcgttgttca tgctgatgtg ttttcctctg ctggctcttt
     3361 ttttatggct gccacagtgg cgaagtcagc aacatgcaaa tttgagtacc tcacgcgcct
     3421 tacatactcg gggtatctgg cgttcgccac ttgcctggca ggtaacattg tttcttggga
     3481 tcaactcact ggtctattac gtgattattg gctggcttcc ggcgatcctc atcagtcacg
     3541 gctatagcga agcacaggcg ggttcactgc atggtttgct gcaactagcc acagcagctc
     3601 ccggtttgct gatcgcactt ttcttacatc atgtgaaaga ccagcgtggt attgcagcgt
     3661 tcgttgcctt gatgtgcgca gtgggcgcgg ttgggctctg ctttatgcca gcgcacgcga
     3721 tcacctggac tctgcttttc ggttttggtt ccggcgcaac aatgatactg gggttgacgt
     3781 tcattggtct tcggcaagtt ctgcgcatca ggcggcggca ctctcgggga tggcacaatc
     3841 cgtcgggtat ttgctggcag cctgtgggcc gccgctaatg ggtaaaatac acgatgctaa
     3901 cggtaactgg tccgtaccac tgatgggtgt tgcaatactt tcactactga tggcgatttt
     3961 cggactttgc gccgggagag acaaagaaat tcgctaatat ccggtgctat agtgacgtga
     4021 caaatcttgc gtgaaaggga gaacaaacac gatgaatatt cagtgcaaac gcgtttatga
     4081 tccggctgaa cagagcgatg gttatcgcgt actggtcgac cgcctctggc cgcgcggtat
     4141 caaaaaaacc gatttagccc ttgatgagtg ggataaagaa atcacgccgt caacggaact
     4201 gcgcaaagcc tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc aatatcgcgc
     4261 agaactggcg caacacgagc aagaaggaaa gcggctggcg gacatcgcca aaaaacagcc
     4321 gctgaccctg ctctacgcag caaaaaacac cacgcagaac catgcgctgg tgctggccga
     4381 ctggctacgt agcttgtgat tttagtacgg catccggcgg ttatttttca ccagccggat
     4441 ggtcccgtcg ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt
     4501 tgctgacccc ttgcgctgtc tgcactggaa tgagcgtccc gcccttctgc tggcaataga
     4561 ccgacgccgg atttgccata ccaatctgcg gcggtttagg tgcttctggc tgagaagggg
     4621 ttgaacaacc agccaggacc agcaagcaag gcagaacaaa actgataatt ttcatttatt
     4681 gatctcacat atttatccaa gcttagagta tcgcggtatc gttttgtttt gcagcactat
     4741 ttttattaca ttcactcaaa acatattacg tcttgtttca tctttgttga tgatgtttta
     4801 tcatgcctgc aaagattaaa taatcagcac ttacccgccg tatcctggag ttgttccgtg
     4861 tcagatcaga ttatcgcccg cgtctcgcaa tcccttgcca aagaacagtc actggaaagt
     4921 ctggtccgac agcttctgga gatgctggaa atggtcactg atatggaatc aacctacctg
     4981 accaaagtgg atgtcgaagc gcgcctgcag catattatgt ttgcccgtaa cagccagaaa
     5041 atgcacatcc cggagaattt taccgtcttg tgggattact cgttatgcaa acgcgccatt
     5101 gatgaaaact gctttttcag cgatgaagtc cctgaccgtt ggggcgactg tattgcggca
     5161 cgcaatcttg gcatcaccac atttctgagc acgccaattc acttaccgga tggatcattc
     5221 tatggcacgc tttgcgccgc cagcagtgag aagcgccagt ggagtgaacg cgcggaacag
     5281 gttttacagt tattcgccgg actgattgca caatatattc aaaaagaggc actggttgaa
     5341 cagctgcacg aagccaatgc tgcgctgatt gcgcaatcgt ataccgactc gttaaccggg
     5401 ctaccgaatc ggcgggcgat ttttgaaaat ctgacgacgc tgttttccct cgcccggcat
     5461 cttaaccata agataatgat cgcgtttatc gatctggata acttcaaatt aatcaatgat
     5521 cgttttggtc ataatagtgg cgatctgttt ctcattcagg ttggcgagcg ccttaatacg
     5581 ctccagcaaa atggcgaagt tattggtcgt ctcggtggtg atgagttttt ggttgtttca
     5641 ctaaacaacg agaatgcgga tatttcgtcg ctgcgagaac gtattcaaca gcaaatacgt
     5701 ggagaatatc acttaggtga tgttgatttg tattatcccg gtgccagtct tggcatagta
     5761 gaagtcgatc ctgaaacaac cgatgcagac agtgccctgc atgctgccga tattgcgatg
     5821 tatcaggaga aaaaacacaa acagaaaaca ccttttgtcg cgcatccagc gctacattcc
     5881 tgaggcgtat tcacatcctt ttgattggtg ataacatgcg aatcggtatt atttttccgg
     5941 ttgtaatctt cattacagcg gtcgtatttt tagcatggtt ttttattggc tgctatgctg
     6001 ccccgggagc ataaagatga aaaaaacaac gattattatg atgggtgtgg cgattattgt
     6061 cgtactcggc actgagctgg gatggtggta acgtcacctc taaaaaatag caaaggctgc
     6121 ctgtgtgcag cctttgtgca atttaagcgt taacttttaa tcttcctgta gataaatagc
     6181 acgacaatcg caccaataac ggctgaagcg gcgcacgaaa aacgctttcg cgtttttcgt
     6241 gcgccgcttc acccctggtt cagcatttcg tctgcagtct gaaccgcagg caaccatttt
     6301 tcagcaagtc tgcgctggga atcgctggct ttatcccatt tgctccacag tgcctcacga
     6361 tcgtaatcgt gcgcttccag cgtggggcgg gcgatttcaa aataccccta gcttgtttct
     6421 atttgctca
//
LOCUS       DAJTYH010000168         6419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTYH010000168 DAJTYH010000000
VERSION     DAJTYH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..2347
                     /locus_tag="OO609_003779"
     CDS             <1..2347
                     /locus_tag="OO609_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6635020.1"
                     /translation="NTTQIRQEVKLRDGKVIVRGKVAARRTVKSADIVLYHKPGIPLA
                     VIEAKANKHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAEGECLEKQITLD
                     DFPSPAELWQKFCLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAINKTIEAVSNGQ
                     NRVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQPFGTAM
                     TKVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSA
                     WREILDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRV
                     DIDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRTN
                     PMDKTIVFCNDIDHAERMRRALVNLNPQQVKKNDKYVMKITGDDEIGKAQLDNFINPK
                     KPYPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTI
                     LDFKKATELFADERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEP
                     PAPPYQVTDTDDVGPFPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDY
                     TRKTLLKEYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCH
                     VVYGQPPLTRKERAENVHKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLK
                     PFDSMGTLPEIIKTGFGDRNGYNQALSELENEIYQLPPRSA"
     gene            2414..3883
                     /locus_tag="OO609_003780"
     CDS             2414..3883
                     /locus_tag="OO609_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6635021.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            3883..5544
                     /locus_tag="OO609_003781"
     CDS             3883..5544
                     /locus_tag="OO609_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6635022.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5765..6106
                     /gene="symE"
                     /locus_tag="OO609_003782"
     CDS             5765..6106
                     /gene="symE"
                     /locus_tag="OO609_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6635023.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6153..>6419)
                     /locus_tag="OO609_003783"
     CDS             complement(6153..>6419)
                     /locus_tag="OO609_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635024.1"
                     /translation="QEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFHELVDPKGIA
                     KDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
BASE COUNT         1889 a         1530 c         1482 g         1518 t
ORIGIN      
        1 caacacaacg caaatcagac aggaggtcaa actccgggac ggtaaagtca tcgtacgtgg
       61 taaagttgcg gcacgcagaa cggtaaaatc tgctgacatc gtgctgtacc acaagccagg
      121 cattccctta gccgtgattg aagcaaaagc caacaaacat gaaattggca aagggatgca
      181 acagggcatt gaatatgcgc gcctgctgga cgttcctttt gtttttgcca ccaacggtga
      241 tggctttatc ttccgcgatg ccaccgcagc cgaaggtgaa tgccttgaaa agcaaataac
      301 gctggatgac ttcccctccc ccgctgaact ctggcaaaaa ttctgcctct ggaaaggata
      361 cacacaagct cagcttccgg tgattactca ggactattac gacgatggca gcggcaaatc
      421 gcctcgttat tatcaacttc aggcaatcaa caaaaccatt gaagccgtct ccaacgggca
      481 aaaccgcgtt ctgctggtca tggcgaccgg aacagggaaa acctataccg cattccagat
      541 catctggcgc ctgtggaaat caaaaaataa aaaacgcatt ttgttccttg ccgatcgcaa
      601 cattctggtc gaccaaacca aaaataatga tttccagcca tttggtacgg caatgaccaa
      661 agtcagcgga cgcaccattg atcccgctta tgaaattcac ctcgcgctct atcaggctat
      721 aactggcccg gaggaagacc aaaaagcgtt taaacaagtc gcaccagatt tcttcgatct
      781 gatcgtgatc gacgaatgcc atcgcggcag cgcatccgaa gacagcgcct ggcgagagat
      841 ccttgattat ttcagttccg ccacccaaat tggcttaacc gccacgccaa aagagacgca
      901 tgaagtctcc agcacggatt acttcggcga tccggtttac gtctactcac taaaagaagg
      961 gatcgaagac ggcttcctcg ctccttataa agttgtccgt gttgatattg atgtcgacct
     1021 gcaaggctgg cgcccaacca aagggcaaac tgacttaaac ggcgaagtga ttgacgatcg
     1081 tatctataac cagaaagatt tcgatcgcac gatggtgatc gacgaacgca cagaactggt
     1141 tgccagaacc attaccgact acctcaagcg caccaatccg atggataaaa ccatcgtctt
     1201 ctgtaacgac atcgatcatg cagaacgtat gcgccgcgcc ctggttaatc tcaacccgca
     1261 gcaggtgaaa aagaacgaca aatacgtcat gaaaatcacc ggcgatgatg aaattggcaa
     1321 agctcagttg gataacttca tcaacccgaa aaaaccgtac ccggttatcg cgaccacttc
     1381 agagctgatg accactggtg tggatgctaa aacctgcaaa ctggtagtac tagaccagaa
     1441 catccagtcg atgaccaaat tcaagcaaat tatcggtcgt ggtacacgca tcgacgaacg
     1501 ttacggcaaa ctctggttta ccatcctcga ctttaaaaaa gccaccgaac tgtttgccga
     1561 tgagcgtttc gatggcattc ccgaaaaagt catggatacc acaccagagg atatcgccga
     1621 tccagaatct gattttgaag agaaactcga agaaatcagc gaacatgacg acgaacaggt
     1681 aacaggcgtt gatgaaccgc ctgcgccacc ataccaggtt accgataccg atgatgtcgg
     1741 cccatttccg gaagaagacg agaagaaaat ccgcaagttt cacgtcaacg gtgtagcggt
     1801 gggcgttatt gcacagcgcg ttcagtatta cgacgccgac ggtaaactgg ttaccgaatc
     1861 ctttaaagat tacacccgca aaacactgct caaagaatat gcctcgctgg atgactttac
     1921 ccgcaagtgg caggacgccg atcgcaaaga agcgatcatt cacgagctgg agcaacaggg
     1981 gatcatctgg gaagtactgg cagaagaagt cggtaaagat ctcgacccgt tcgacatgct
     2041 ttgccacgta gtgtatggtc agccgccgtt aacccgcaaa gagcgcgccg agaacgtgca
     2101 caagcggaac tacttcacaa aatactctga agcagcgcaa gccgtgctcg ataatctgct
     2161 ggataaatac gccgatgcgg gcgtacagga gatcgaaagt attcaggtgc tgaaacttaa
     2221 accattcgac agcatgggca ccttaccgga gattattaaa accggatttg gcgaccgtaa
     2281 cgggtataat caggcgctca gcgagctgga aaacgaaatc taccaattac cgccccgctc
     2341 tgcttaacaa ctctcttttg cgccttctgc aaccatcatg ttgcagaagg ccttgttttt
     2401 catggaatac aacatgtcta tcagctcagt aatcaaatca ttacaagata ttatgcgcaa
     2461 agatgccggt gtggacggcg atgcgcagcg tctcggtcag ctctcctggc tgctgttttt
     2521 gaaaatcttc gatgcccagg aagaggcgct ggaactggag caggataact atcaatatcc
     2581 gatcccacag cgttatttgt ggcgcagttg ggccgccaac gcgcagggca ttaccggtga
     2641 ttccctgctg gaattcgtta atgatgatct gtttccggcg ttgaaaaacc tcactgcgcc
     2701 tatcgataaa aacccacgcg gctacgtggt gaagcaggcg ttcagcgatg cctataacta
     2761 tatgaaaaac ggtacgctac tgcgccaggt gatcaacaag ctgaacgaaa ttgactttac
     2821 cagcgccagc gaacgccatc tgtttggtga tatttacgaa cagatcctta aagatctgca
     2881 atctgcgggc aatgcgggcg aattctatac tccacgcgcc gtcactcgct ttatggtgga
     2941 tcgcgttgat ccgaaactcg gcgaatccat tatggacccg gcctgcggta cgggcggttt
     3001 tcttgcctgc gcgttcgatc atgtaaagaa caaatacgtg aagagcgtcg ccgatcatca
     3061 gacactgcaa caacagatcc acggtgttga gaaaaaacag cttccgcacc tgctggcgac
     3121 caccaatatg ctgctgcacg gcattgaagt accggtacaa attcgtcacg acaacaccct
     3181 gaataaaccg ctttcctcct gggatgagca actggatgtc attgttacca acccgccgtt
     3241 tggtggcacg gaagaagacg gtattgagaa gaactttccg gcagagatgc aaacccgcga
     3301 aacggcggat ctgtttctgc aactgattgt ggaagtactg gcgaaaaacg gtcgtgcggc
     3361 ggtggtattg ccggatggca cactatttgg cgaaggtgtt aaaaccaaaa tcaaaaagct
     3421 acttaccgaa gagtgcaacc tgcataccat cgtgcgttta ccgaatggtg tgtttaatcc
     3481 ctataccggc attaaaacca acctgttgtt ctttaccaaa ggtcagccaa ccaaagagat
     3541 ttggttctat gaacatccgt atccggcggg cgtgaaaaac tacagcaaaa ccaagccgat
     3601 gaagtttgaa gagtttcagg cggagatcga ctggtggggt aacgaggccg atggttttgc
     3661 cagccgcgtc gagaatgagc aagcgtggaa agtcagcatt gatgacgtga ttgcgcgcaa
     3721 cttcaatctg gatattaaaa acccacatca ggcggaaacc gtcagccatg atccggacga
     3781 actgttagcg cagtatgcaa aacagcaggc ggagatccag acgctgcgta atcaactgcg
     3841 cgatattctt ggcgctgcgc tgtctggcaa ggaggttaac taatgagtgt ggaaaagctg
     3901 atcgttgatc atatggaaac ctggacctcg gcgttgcaaa cccgttccac cgccgggcgc
     3961 ggcagctccg gtaagattga tttgtatggt attaagaaat tgcgtgagct gattctggaa
     4021 ctggcggtgc gcggtaaact ggtgccgcag gatccgaatg atgaaccggc gtcggacctg
     4081 ctgaagcgta ttgcggcgga aaaagcagag ctggtgaagc aaggcaaaat taaaaagcaa
     4141 aaaccactgc cagaaattag tgaggaagag aagccgtttg aattgccgga gggatgggag
     4201 tgggtgcacc tccctgatat ttattgctca atttctgagt catctagaaa aataaaatcc
     4261 tccgaaatac tacctgaggg aaaatatcct gttatcgagc aaagccaaga atttatttct
     4321 ggttattgca acaatgaatg tctgttaata aagttaaata atccagttat agtttttggt
     4381 gatcacacaa ggaatattaa atttattgat tttgattttg ttgtcggcgc tgacggtgtg
     4441 aagattttat cacctatatt aatttgtgag cgtttctttt tttggcagtt acgatcattt
     4501 aaattagatg taagaggata cgcgagacat tttaaagtat taaatagctg cttatttgca
     4561 ttacccccca ttgcagaaca agagcgaata gttgaaaaag ttagctcttt gatgtccctc
     4621 tgcgaccaac tagaacagca atccctgacc agtctggacg cacatcagca actggttgaa
     4681 accctgttgg gaacgcttac agacagccaa aacaccgcgg aactggctga aaactgggcg
     4741 cgtattagcg agcatttcga caccctattt accaccgaag ccagcgtgga tgcgttaaaa
     4801 cagaccattc tgcaactggc cgtaatgggc aaacttgtgc cgcaggatcc gaatgacgaa
     4861 cccgcctctg aactgctcaa acgtattgcg caggaaaaag ctcaattggt gaaagaaggg
     4921 aaaataaaaa aacaaaaacc actgccgcca attagcgatg aggaaaaacc gtttgaactt
     4981 ccggaagggt gggaatggtg tcgtattggt aatatagtta atattaaatc tgaattagta
     5041 tcaccaaaag attatttaaa tttatatcaa gtagcacctg atattattga aaagggcact
     5101 gggagagtga tatcaaagag aacagtgaaa gaatcaggag tcaaagggcc aaatagtcgc
     5161 ttttataaag gacaaattgt ttattctaaa atccgcccgt cattatccaa agttttttta
     5221 gcagagtata atggtctttg cagtgctgat atgtatccat tagactgtta cattaatcca
     5281 aattacttat tgaaatatat cctttccatt ccatttctta tgcaagtcaa aaaagcagag
     5341 aacagaataa aaatgccaaa acttaattct gacagttttt acaatattat tgttgcgatt
     5401 cccccctata atgaacaaca agcaatattt gataagataa attctattga agcagtatgc
     5461 aatggtctta tttcttatat tgggatttat cataaaaccc aactgcatct tgcagacgca
     5521 ctcactgacg cagcgataaa ctaattccct cgccctcaca aaaccaataa agcggctaaa
     5581 tagccgcttt attcacatca ccaaaattta tatttcacgc taattttcat cttattttca
     5641 ttaattttta gctggacttt ccctgtattt actgatgatg tatccaggta tttagcacgg
     5701 tgcggatgtg cgccaacaca ctcgcaccgc taatcacaat cactatttat agagaatagt
     5761 aatcatgact gacacgcatt ctattgcaca accgttagaa gcagaagtct ccccggcaaa
     5821 taatcgtcaa ttaaccgtga gttatgcgag tcgttacccg gattacagcc gtattcccgc
     5881 catcaccctg aaaggtcagt ggctggaagc cgccggtttt gccaccggca cggcggtaga
     5941 tgtcaaagtg atggaaggct gtattgtcct caccgcccaa cccgccgccg ccgaagagag
     6001 cgaactgatg cagtcactac gcaaggtgtg taagctgtcg gcgcgtaaac aaaggcaggt
     6061 acaggaattt attggggtaa ttacaggtaa acagaaagtc gcctgataca ttgacccggt
     6121 agcgccagtg ctaccgggta ttattcgccg cgttatacca acgcgctctc catctgtttt
     6181 tcaaatgcct gacgaattaa tcccataatg taaggaagtt gtgcgaggtc gctgaaacca
     6241 atttctacat cgccattacc ccagcgccca acatttgtca catctttggc aatacctttc
     6301 ggatcgacca gttcatgaaa ctgcatatta agcgacaatc gcagtcggct tttttgcggc
     6361 acaacatcaa caaaattcgt ttccgcttta aacgcaatat atagcttcaa tacttcctg
//
LOCUS       DAJTYH010000169         6049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTYH010000169 DAJTYH010000000
VERSION     DAJTYH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            86..496
                     /locus_tag="OO609_003784"
                     /pseudo
     CDS             86..496
                     /locus_tag="OO609_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            528..1142
                     /gene="istB"
                     /locus_tag="OO609_003785"
                     /pseudo
     CDS             528..1142
                     /gene="istB"
                     /locus_tag="OO609_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1311..5405)
                     /gene="sepA"
                     /locus_tag="OO609_003786"
     CDS             complement(1311..5405)
                     /gene="sepA"
                     /locus_tag="OO609_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6635025.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5807..5995
                     /locus_tag="OO609_003787"
     CDS             5807..5995
                     /locus_tag="OO609_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635026.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
BASE COUNT         1477 a         1534 c         1443 g         1595 t
ORIGIN      
        1 gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact
       61 ctccaggaaa cccggggcgg ttcatgccgt caggcagcag cgaagcaaag cgttactgac
      121 gtcgctgaat gaatggatgg tggagaagaa tggtacgctg tcgaaaaaat ccagactggg
      181 cgaagcgttc agctatgtac tgaatcagtg ggatgccctc tgttattaca gtgatgacgg
      241 tctggcggag gcggacaata acacagcaga aagagcgctt cgtaccgtct gtctcgggaa
      301 aaagaattac atgttcttcg gcagcgatca tggaggcgat cgcggagctc tgctgtacgg
      361 actaatcggg agctgtcggt tgaacggtat cgatccagaa gcgtatctgc gccatatcct
      421 gagcgtactg ccggaatggc cttccaaccg agttgacgaa ctgctgccat ggaacgtagt
      481 tctcaccgat aaataagcgt caatacggcc ttccgttgac gcttacagaa ctcctgtggc
      541 gcgagagtga aaaactgcgt cgttataaaa aggaggcccg gttgccagtt gccaaaacgc
      601 taagcgaata cgacttcatt cagttgccgg aactgaatgg agctcagttc cagcaactct
      661 gtgaaacgac agactgggtt gatgctggag aaaacgttct gctgttcggg gccagcgggt
      721 tggggaaaag ccatctggcg gcagcgatcg tggatggcgt agtaggccag ggctaccggg
      781 cccggttcta cagcgcagga gagctgttgc aggaactacg taaagccaga gctcagttga
      841 aactgaatga gctgctactg aaactggatc gctaccgggt gatagtggtg gatgatcttg
      901 gctatgtcaa acgcgacagc gccgaaacgg gtgtgctgtt cgagttaata gcgcatcgct
      961 atgaacgtgg gagcctggtg ataaccagta accatccgtt cagcatgtgg ggcagcatct
     1021 tcgtggatga gactatggcg gtggcggcag cagaccgact gatccatcac ggatatatgt
     1081 tcgaactgaa aggggaaagc tacaggaaaa agacagcgaa ggcagtaaca agcgtgactt
     1141 gatgtcgcac tgaaggggcg gccagtatag ttggcgccag tcggcaaaac tagttgacgt
     1201 ctaatacctg gacggtcact tctgagaaaa ggcatttaca cagaatctta aacaggctca
     1261 cggagttctt tcagaaaaag gcctgccgaa tggcagacct atcccattcg tcagaaaaca
     1321 taacggaagt tggcgtttat cgcattatcc acattatatt tcccgaacgc tgatttctcc
     1381 agctccagtc ccaaacgcat attatcctta atttccgcat tcatccctac cgtcatcagc
     1441 atcctgctat ctttttcacc ttcgaaacgt ttctctccgg aagcatcctg cagaaccgtt
     1501 tctccgttcg ccagcaggtc gaactgataa cccagcccgg ctcgagctgt gattttccag
     1561 tcgtctccgg agaaggctct tcccacgtca acaccagtac ggccaatcag tgggttataa
     1621 tccttgtctt tcatgctcag agccattccc cggtcttccc agctaaaagc ttttcctgat
     1681 acagaaccgt aaaccagctc tatctgtggc tccacccagg actctttcgt caggtggtaa
     1741 cgataaccaa cttcagcacc ggcataccag gaatgagagc tgtagtcttt tgttcccagt
     1801 gaggcaaaat tcgccgtgtg ctgattatca tgatggagat atttaccaat caggtcaaaa
     1861 taagctccgg aattaaagag tgcagaggca tacagaccgc cacccacgga tttgtttttt
     1921 ccactgaatg cgtggctgct tgcattgctg tccgtatagg tcagcaatgc ccccgtaaat
     1981 aaatccacac cgtccagctc atgctttctg tcgacaccaa tctgaacgtg agtgtagtta
     2041 tcgctgtagt caccatctgc cgaaccggta ccattcatta tgcgtgccca gacaccggca
     2101 tccccctgag tatcccgcag gtcacccata cgtttgttca ggttattgac ctctgtaagg
     2161 aaggacttat aacctgatgc cataaagtct tttgcggctt tcgaggctcc ggcatcagcg
     2221 gtagtctgat atcctgtcag cacccatttt gtggcatcat ccgttttttc cgtgctgatt
     2281 actggcgtaa cgttactgaa accaatctgc tgcgtaccgg cagtgaacgt tttctcatca
     2341 ctccctttcg gtgcggtaat taacgtcacg ctcatttcac tggctggttt gtttaaaaag
     2401 tcaaccagga cgctattatt actgcctgac agtttgtctg tgacattcaa ctggtcagcc
     2461 cggctgttgt tggtatgcat caggaacgtg gtattatctg cagaaagctc ttttacttgt
     2521 aaagtggcaa agtgtttccc gtcccccagt tcaattcgcg agcccttagc cttcagtgca
     2581 ccggcctctg attttccgtc agagtaccac agagcattga tcatatttac cgtactctgt
     2641 gcccccgtaa tactgcctct cagaacagcg ttatatccct tcattaagga gctgaataac
     2701 ggagagtccg tcttccctgc atcagcatca ctgtctccca gattaatggt tgccgcagtg
     2761 gttcctgcat gaatatccga actcagatag cccatatttt taacagaaaa gctggcgtta
     2821 tcttcgaggt taatcccttc tgttgtagaa attacgggtg agtaataccc ctgtttctgc
     2881 gcagaaggca tccccgtcag ggtcagagaa gcattttctt ttacatccac agccccggtg
     2941 ctgaaaatac cgctctgagc cgtaagtttt gctccttttc cgagggtcag gctgctgttg
     3001 acaaagctgc caacacggtc aaaaacagca ttctgagagg tcacactcac ggtgctatca
     3061 taagcctcaa taccaccggt gaatctctcc atgatttgca gggatgattt attttccagc
     3121 gtaacatttc cgctgtaatc actctggtca ccaactgtgg ttgcagtggc actcccttca
     3181 acagaggaga ccgcattacc ggttccgtca ctcctgtcag taaagacacg gctacttccc
     3241 agaatgactg tacttccatt ggtggcgtta atatctccct gcatcttgcc gtttctggac
     3301 agatggaatg ttgccgcatc gagtttcagt ttttccagcg taaactgacg ggtttcccag
     3361 tcgctctggc tggaagaagt cgtcgttcca gcatgaataa ctggatgacc ctggaatatc
     3421 agcgtgccgg acttcttggt tatcgaacct tctggcaggt taactgaacc atcaagagcc
     3481 agtacatcct tacctgagag ctgcggaata ttcacatcca tattgccgtt tagttggccg
     3541 tgatacatat aaggctggct gctggcctca attttttgtt gcttcacagt atcaatggct
     3601 ttattacgat cttttccgac attctgccag acactgctgt tgttcagatc ggagaagaac
     3661 ggggaatatg aactcgcttt caggatgaaa tattgcttgg tagaactgtc ataatatagg
     3721 tctcccggcg ctcctctccc cccaaaaata ctgacagtat taaccggtac attgatgtca
     3781 ctggctttta atgtctgaag gtcgagtgtt accgttgatt ttttctcact gttattggta
     3841 atgatggccc cgtagtcagc cgcctgaaga cgggtaaagg tcagattatt accattcagt
     3901 tccagacgtc ctccacggta tccccacgaa atattatccg gattaacttg ctgtgagtca
     3961 gaaagcacaa ctgtcggacg accgctggca ataccaacag agctgaaagc ctgtaccttt
     4021 ccgtcagcat ccgcctgctg gttgagaata acggtaccgt cccccacctt gagtccgcct
     4081 gcattcacac cggtcccgtt tactgtcagg gtaccttcgc cagttttatg caggttatca
     4141 ccggcaactc cgttcacctg ccacagaacg tgggttcctt tatcggtgat aatcccgccc
     4201 cccatccatg tctggccgtt cagggcggac accctgtaat cgccggcaaa ctgcagatac
     4261 ccagctccct gattaacgga attctgcaga acgacctcgc cattattgcc ggtgaacagg
     4321 aggttttttc ctgcgttcag atcatttcct ttctttccat gcatatccca agtcgtgttt
     4381 ccctgagtca gtgtgccggt gccattagcc gcatcatatt tccactgcag tactccttca
     4441 ccagaggtgt aggctatagt tttgtcaaaa tcattttgcg gttgctgacc aaggaaatcc
     4501 tgagtggtga cgacccagtt atttccatag ttagttcctg aggaaagcac gccaatcagt
     4561 acccattttt tttgcaggga atcataagca aacagggggg agccgctgtc ccccatattc
     4621 agataattag ccagaggtcc attcgcggga ttaaaaatgt caccggtctg agctgtaatc
     4681 atctgtccgt tgttatagga gttcagcgcg ctgacggttc ccccaacaag aaatccgcca
     4741 ttggtataca cttgggtgcg attaccattt ttgtctttga cgtactgcat ccccccccca
     4801 aggcgataga attcagtaaa gcgtccgccg gcctgatagg caccgctcac agccccgatg
     4861 tcagacactt ctgccggcgc cacctctgtc accagtttac tcaggcggcg ggttttaata
     4921 tcaagaccgc tattattatt tgtaccaacc gcagtatagg tgtttttggc gtatccgaag
     4981 ctcatcgtgt cagaaccgct gacatgtttg gcggtaacca catactgagg ggagtaaagt
     5041 gtatggtctc ccggaggcag gctaccggta gtaatagtgg cactactgaa atcagccatc
     5101 ggggctttat caagtttacc gaccagattg ccctgcttgt cgtaaatgga aatattcgtg
     5161 gtccccggcg taaactgacc tttattctcg gcaaagtcgc ggaatatctg ataagggatc
     5221 tctgcactga ccgttgctga cagagctggc catgccgagg aaagtgataa agctgcaaca
     5281 gacgtaagaa taacccgacg tgaaagtctg cgatggcttt tgcaggtaac cctgcgggcc
     5341 agttcggaga cagcaatcag gcttttggtt atatggcaat acttaagata atatattttg
     5401 ttcatataga aacatcaacc taattttgac aaagattaat tacataggat gtagatggag
     5461 cagtccgaga ttctactaat atcaattatt aagcatctgt tttttagttg gtgtaatata
     5521 atttttatta aggtaagcgg aaaaacaacc aaaaaaaacg caagatgtta tttttttatc
     5581 attttgagcc agtttggctg gggggatact cttctcttaa gcttcttgct caactttaaa
     5641 aatagttatg ccagttcggt atcgataagg ggcagtttgg atatcgagaa ccacggcagc
     5701 ctgacggctg caaattgaaa actgcccctg ttcgtcctgg tgttgccgca cgctccgcag
     5761 aatatgtatg ctctgaggac ctgttttatt aaggactcat cctcttatgt tgacccgcaa
     5821 aagtatagat accgttctgc tctctgttgg tgctgagaag ctctcacaac gtgaatggga
     5881 ctggatgaag atgctcaaac ccatggaccc accacctgcg atggtcgcgg cctccatcct
     5941 ggagcgccgt ggcgacacag cagcccttac ccggctacag gataccgggg gttaatccgt
     6001 cctcatgttg tccgctttta ctccccgccc gctgaaacgt ctgttcacg
//
LOCUS       DAJTYH010000170         5954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTYH010000170 DAJTYH010000000
VERSION     DAJTYH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..364)
                     /locus_tag="OO609_003788"
                     /pseudo
     CDS             complement(1..364)
                     /locus_tag="OO609_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(550..3858)
                     /gene="icsA"
                     /locus_tag="OO609_003789"
                     /gene_synonym="virG"
     CDS             complement(550..3858)
                     /gene="icsA"
                     /locus_tag="OO609_003789"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6635027.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4387..5589
                     /gene="virA"
                     /locus_tag="OO609_003790"
     CDS             4387..5589
                     /gene="virA"
                     /locus_tag="OO609_003790"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6635028.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5768..>5954
                     /locus_tag="OO609_003791"
     CDS             5768..>5954
                     /locus_tag="OO609_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6635029.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1836 a         1347 c         1011 g         1760 t
ORIGIN      
        1 cctcgccact tcaccgggct caggaccccg taggatcttc accgaaaaaa atgcatcacc
       61 agttgcttta ttatttgttt taggtagacc tgttactaat ttatacctac tctgaccaag
      121 tctggataag aaaggcacac ctgcacattt ctacttggaa atgttttctt ttaccatttt
      181 gctcaaaaat ttcaacatcc aatattcctc gtacagaact actcaagtcc tgaatagaaa
      241 aagttccagc tggtacagta gagtcatata ttactcgacc ttgttgagat acaacaacac
      301 gtgcattagt ttctgcaata ccagtaatct gaggcgcata accacgaata tttggcggta
      361 gcattcgttc atcgctttct aatgcaattc ctgtgtaacg ccaagcaaca gcgcattctg
      421 gcgcatgatc tggtaaacac gtttggcatt gatcgcaggc ataccatcat gtgcacaaaa
      481 acccaccgag cttttacacg gtgggtccca gagaaatgca ggacatcaac acgccctgca
      541 tttttattat cagaaggtat atttcacacc caaaatacct tgggtgtctc tgtaactgtt
      601 attacccaat tgctgagata cgtttcccca gatgcttagg ttatctgcaa gacgcccccc
      661 cacaccagtt ttgagttcaa tcacattacg gttgcctatc tggtgatttg acatgccatt
      721 cataattaca ccatattgct ttgagctcca tttccaattc acttccaaaa atggttcgaa
      781 cttacgaaga gtctccttat cgatacaact ctgactaatt aaggatgtac gcaatcccaa
      841 ttttgcttga atattattgc ccccagagga tgtgaccata gtgccattgt gttcaatata
      901 gtcgtttgct tttaccccat gcctagttat ctggacttgt ggattgagat acaatgcgtt
      961 gtcaatatta ttatgagcag tccagcgtat ggttgggtag atatagccag cttccaaagc
     1021 tcctgttatt cctttgctgg aatatttctc accagttaac ccatctcctt tgactgtatt
     1081 attaaaccaa ctgtactgca accaagcatc catatataga cctgttcgtt ctttttcatc
     1141 ctgaaaccaa gaactgtaca aaccggcagt ataaccgctc acagttcctc gtgaactata
     1201 actagtatac ttattatgag ttttgctatc ctgatatcca taagctccca taatacccac
     1261 atgcaagcca tcatgatcag tgaagtttgt cttcaaaata tctccaccaa tctgataaac
     1321 catcctgtta gctgtggttt ttaattgtct atcagctaaa ttatgctcat tatgtccacc
     1381 aacagtacga atccacatgg tggtttcgct gagctgacct gtagtaggat caacaatagt
     1441 gctaccgtgt cggtcataga gagaaggact aaacatcgtg ttcattgcta tcaggttagc
     1501 aatataactg ccagcttcag ggcgccatgt gtgaatacta ccttcaccca aattaagtga
     1561 agatacaagc tgtgaactag ctcggcgact actcatttga gtagactctt gattgctcat
     1621 ctgttttgat tcttgattat ccatctgact agttagatac cacttattgg tattcagtcc
     1681 agatacagtg ccctgtttca ggcgatagtc ataactacca gcaacaatac ggcctttctg
     1741 aataaaagca ttcttatcag aagaatctgt cgaaataatt tgaacacctt caagagtttt
     1801 atctccagag cctccctcat ttgtcactgc aacataggta ataccagacg catggcctgc
     1861 aatttccagc cggtcagttt tactgttgtc attgccgaga accgcgccca aagataatat
     1921 tccgcccttt ccatgccaat tacctttctg aacataggtc tgaccgacat ttgacgaact
     1981 attattgaga ataacatgac cgtttttctc cagagtcatg ttacctgtga caatgacggg
     2041 gtcaggcaaa aaaggggcat taatattatt gattagcaca gtcccactat ttaataaagt
     2101 gttaacagtt tggttcatgc ctgaaaaatt caaggtagca cctttattaa caataacacc
     2161 agcggtacgt gtcatagctt caactgtccc catttttaga atgcctgcat tgatgttggt
     2221 gaaaccagag taggtatttt tttccgccaa aatgagagtt ccctcccctg attttgttag
     2281 ggattttccg tcccagctat ttatattatg atggctgaga ttatctgcca gaatagtact
     2341 aacagtaagt tcagcgtttt ctttcagatt gaactctcca tgactgtcgc catctgtatc
     2401 attccagctc aaaccgtaga tcaatttatt ctggtcactg atatagcttt ttgtatagtc
     2461 aatagcacca gtagtgagta acggattgat gatagatata ttagaaacac tgaaacctgt
     2521 tatcccttta tctgcactta gcagtttata gtccttctgc gcccagttat ttataatagc
     2581 ctccactttt acattgttgc caaatgacat atttccagaa gcttccagac tactgattcc
     2641 agctaaattt aaagaactgt tctcaccaaa agaaacgtca cctgaaacag tgaatttgtt
     2701 acctgatagt gaaacagttg tacaatcacc agcacataaa tcaccttcaa tagttggtgt
     2761 ggtcccagtg taatcattgt tgataatttt cagaatggat gaattgttaa cctgtacatc
     2821 accagtaatg acagaacctt catggagtat taatgaattg tttccaccag tgatgtggat
     2881 agcattacct gctacatttt gtgttccatc atcttcttta cctgaaatta tatatccgct
     2941 attagcaatg gtcatattgc taccatttat agcatccccc caatgtccac cattaccgcc
     3001 taaaatagct ccattgttga ttacagacag gttatctcct gtgatagcat taccaccgta
     3061 gccatcataa ccataaccgt aagcagcacc tccgttgcct ccagcgaacg tgcccttatt
     3121 gattacagag aggctgcttc ctgtgatggc atcacctcca ttaccgccat caccctcacc
     3181 ataattgtta cctccattac caccggaaat agtccctttg ttgattatgg gtaaattgac
     3241 tcctgtgacg gcatcacctc cattcccccc atcactatca ctataactat cgccaccatg
     3301 tccacctgaa ataatttctc catttattat aaacagattg tctcctgtaa cagcctcacc
     3361 accatcaccg ttatggtcag caccgctacc gccgctacca ccaagaatca tgccttgatt
     3421 gattatagac aagtcagatc cggtaataga gtcaccacca ttaccgccac aactattacc
     3481 atcattatta tcaccagcac caccatgacc tgcgctaatc ataatatcag agttaatccg
     3541 tagcttagtt ctagatgcat gagaggggtt ggaagaagtt atataccacg catccattcc
     3601 atcttcacca gctcccaagg gagaaagtcc atcaacaggt gttaataatt tttcataatt
     3661 gtcctctgaa aaatgaagtt cttgagtacc cgaaagagga gtagcaaaag ctattggccc
     3721 cccgagcaac agggatgcac caacaacaaa aggagaaaaa gacaatttgc atgttttttc
     3781 ctttaccgta ggtaattctc cggccccccc ctgtgaacat tgggtcatat tacaaaaaaa
     3841 tttgtgaatt tgattcatgc actatattat cagtaagtgg ttgataaacc cctgaaaaga
     3901 ttccgtttta tccggaataa agggacgata tatgcaaaaa catattaaac aaagcctcaa
     3961 ccaaaacatg agagctgtgt tatatatcaa tgcaacggga gaaatttacc tgtgtcttgc
     4021 tttgaaaaaa agagggggaa agctatatgt tcgataataa attaataatt ttactatgat
     4081 agtctgcttc agaccgcaac ttttcagttc tttctgtacg cttgcccaaa gtttaaccac
     4141 tttgtttttc tatacaccca atggttctat ctgccccttg atgttaatcc ccaaagccaa
     4201 taacatgaat gtattcatat cacaactttc ctaccagact ctccaatgca ataactgcat
     4261 aattgttttt agtggaaatg tatcgttttc ttaaagagaa gaataacatt ccattttatt
     4321 atgttcccaa tttaattata ttaatatata ttggcattaa taggaaaata catcaggaga
     4381 aatcaaatgc agacatcaaa cataactaac catgaaagaa atgactcatc atggatgtca
     4441 actgtcaaat caactacaga agtaagttgg aataaattga gtttttgtga cattttactg
     4501 aaaataataa ctttcggcat atattctcca cacgaaactc tagccgaaaa acatagcgaa
     4561 aaaaaactaa tggatagttt ctcaccatca ctatcacaag ataaaatgga cggagagttt
     4621 gcccacgcaa acatagatgg catttcaata cggctatgcc ttaacaaagg catatgttcg
     4681 gttttttatc ttgatggaga taaaatacaa tccactcaac tttcatcaaa agaatataat
     4741 aacttgttaa gttcactccc ccccaaacaa ttcaacttag gaaaagtaca tactattaca
     4801 gctccggtat cagggaattt taaaactcac aagcctgcac cagaagttat tgaaacagcc
     4861 attaattgct gtacatcgat aataccaaac gatgattact tccacgttaa agacactgat
     4921 tttaatagtg tttggcatga tatttacaga gatattcgag catctgacag caatagtaca
     4981 aaaatatatt tcaacaatat tgaaatacca ctaaaattaa ttgcagatct aattaatgag
     5041 ttaggaatta atgaattcat tgatagcaaa aaagagcttc aaatgctaag ttacaaccaa
     5101 gtaaataaaa taatcaattc aaacttcccc caacaagacc tatgttttca aacagaaaaa
     5161 cttttgttta cttcactctt tcaagaccct gcatttatat ctgcactaac atctgcattc
     5221 tggcaatctc ttcacatcac gtcttcctct gtggaacaca tatatgccca aattatgtcc
     5281 gaaaacatag aaaacaggct taatttcatg cctgaacaac gagttattaa caattgtgga
     5341 catattataa aaatcaacgc agttgtccct aaaaacgaca ctgcaatctc tgcctctggg
     5401 gggagggctt acgaagttag ctcatcaata ttaccatcgc acataacatg taatggtgtt
     5461 ggtattaata aaatagaaac aagctatctc gttcatgctg gaacacttcc aagcagtgaa
     5521 gggcttcgca atgctatccc tccagaaagt cgtcaagtat catttgccat catatctcct
     5581 gatgtttaag ccaatattat attggctgcc agactgtaaa aaaacgttct tcagaacagc
     5641 tctataaact attaaaccaa agctaaaagg atggggacca atagaatctc agcccatcct
     5701 ttttatatca ttatgaaccg ccccggaaat cctggagact aaactccctg agaaagaggt
     5761 aaacaggatg actaaaaata cacgtttttc ccctgaagtc cgtcaacggg cagttcgtat
     5821 ggttctggaa agtcagggcg aatatgactc acaatgggca gcgatttgtt ccattgcccc
     5881 aaagattggc tgtatactgg aaactctgcg tgtctgggta cgccagcatg agcgggatac
     5941 cgggggcggt gagg
//
LOCUS       DAJTYH010000171         5945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTYH010000171 DAJTYH010000000
VERSION     DAJTYH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1251
                     /gene="narU"
                     /locus_tag="OO609_003792"
     CDS             <1..1251
                     /gene="narU"
                     /locus_tag="OO609_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6635030.1"
                     /translation="TGVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPY
                     SFMVPIFGGRRWPVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASS
                     MGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGS
                     VMSLANAAWIWVPLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLAT
                     FGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLIN
                     FIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIY
                     RVKMKGGSDEQAQREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKV
                     FLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1333..5073
                     /gene="narZ"
                     /locus_tag="OO609_003793"
                     /pseudo
     CDS             1333..5073
                     /gene="narZ"
                     /locus_tag="OO609_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5070..>5945
                     /gene="narH"
                     /locus_tag="OO609_003794"
     CDS             5070..>5945
                     /gene="narH"
                     /locus_tag="OO609_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6635031.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1385 a         1595 c         1597 g         1368 t
ORIGIN      
        1 acaggtgtct ggatgctatt cagcgcagtt accgttaatc tcaataaaat cggttttaat
       61 tttactaccg atcaactctt tttattaacc gcattaccct ccgtttctgg cgcattattg
      121 cgtgttccct actcctttat ggtgcctata ttcggtggac gccgatggcc ggtttttagt
      181 actgcaatcc tgattattcc ttgcgtctgg ctcggaattg ccgtgcaaaa tccgaatact
      241 ccttttggga tatttatcgt tatcgctttg ctatgcggtt ttgcaggtgc aaactttgct
      301 tcgagcatgg gcaatatcag tttcttcttt ccaaaagcca aacaagggag cgctcttggg
      361 attaatggcg gattaggaaa cttaggtgta agtgtaatgc agctggttgc accgctggtc
      421 atttttgtac ctgtatttgc ctttctcggc gtcaatggcg taccgcaggc cgacggttcg
      481 gtgatgtcgc tggcgaatgc cgcatggatt tgggtaccgc tactggcgat tgccacgatc
      541 gccgcctggt cagggatgaa tgatatcgcc agttcacgcg cctcaattgc cgaccagctc
      601 cctgtcttac aacgcctgca tctctggctg ctgagcctgc tttaccttgc caccttcggt
      661 tcgtttatcg gtttttctgc gggttttgcc atgctggcaa aaacccagtt cccggatgtg
      721 aatattctgc gcctggcgtt ctttggccca tttattggtg ccatcgcgcg gtcggttggt
      781 ggtgctattt ccgataaatt cggcggcgtg cgggtgacgt tgatcaactt tatttttatg
      841 gcgattttca gtgccctgct gttccttacc ttaccgggca caggctccgg taatttcatc
      901 gccttttacg ccgtatttat ggggctgttt ctgaccgcgg gtctgggaag tggttctact
      961 ttccagatga tcgccgtcat ctttcgccag ataaccattt atcgggtgaa gatgaaaggc
     1021 ggtagtgatg agcaagctca gagagaagcc gtcaccgaaa cggcggcaac gctgggcttt
     1081 atctcagcca ttggcgcagt gggcggcttt tttattccgc aggcatttgg catgtcgctc
     1141 aatatgaccg gctctccggt cggcgcgatg aaagtgtttt taatcttcta catcgtttgt
     1201 gtgctgctga cctggctggt ttatggtcgg cggaagttca gccaaaaata agagatcgat
     1261 gcctgatgta caaccatgtc gcatccgaca aggcatcaga tcccaattca ttcctggagc
     1321 aggagtcatg tcatgagtaa acttttggat cgctttcgct acttcaaaca aaagggcgaa
     1381 acctttgccg atggtcacgg acaggtgatg catagcaacc gcgactggga ggacagctat
     1441 cgccagcgtt ggcagttcga caaaatcgtg cgttccaccc acggcgttaa ctgtacaggc
     1501 tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga cctgggaaat ccaacagacc
     1561 gactacccgc gcactcgccc tgacctgccc aatcatgaac ctcgcggctg cccgcgtggc
     1621 gcaagttact cctggtatct ttacagcgct aaccgcctga aatacccgct cattcgtaaa
     1681 cgactgattg aactgtggcg cgaagccctc aaacaacaca gcgatccggt actggcgtgg
     1741 gcatcgatta tgaatgatcc gcaaaagtgc ctgagctaca aacaagtgcg tgggcgcggc
     1801 gggtttatcc gctccaactg gcaggaacta aaccagctga ttgccgccgc taacgtctgg
     1861 accatcaaaa cctacggccc ggatcgcgtt gccggtttct cgccgatccc ggcgatgtcg
     1921 atggtttctt acgccgccgg aacgcgttat ctgtcgctgc ttggcggcac ctgttaaagt
     1981 ttctacgact ggtattgcga cctgccgccc gcctcgccga tgacctgggg cgagcaaacc
     2041 gacgtaccgg aatctgccga ctggtataac tccagctaca tcatcgcttg ggggtctaac
     2101 gtaccgcaga cacgtacgcc ggacgcccac ttctttaccg aagtacgcta caaaggcact
     2161 aaaaccatcg ccattacccc tgactactct gaagtggcca aattgtgcga ccagtggctg
     2221 gcaccgaaac aaggcactga tagcgccctg gcgatggcaa tggggcatgt gattttaaaa
     2281 gagtttcatc tcgataatcc cagcgactac tttatcaact actgccgccg ctacagcgac
     2341 atgccgatgc tggtaatgct ggagcctcgc gacgatggta gctacgttcc cgggcgcatg
     2401 atccgcgcat ctgacctggt ggatggactg ggcgaaagca acaatccgca gtggaaaacc
     2461 gtagcagtta ataccgcagg tgaattggta gtgccgaacg gttcgattgg tttccgctgg
     2521 ggagaaaaag gcaaatggaa tctggaatcc attgccgccg gtacggaaac cgaattgtcg
     2581 ttaaccctgc tcggtcaaca tgacgctgtt gcaggcgtgg ccttccccta ctttggcggc
     2641 attgaaaatc cgcattttcg cagcgtaaaa cacaatccgg tgctggtgcg ccaattgccc
     2701 gttaaaaacc tgacgttagt cgatggcaac acctgtccgg tggtcagcgt ttatgatttg
     2761 gtactggcga attacggcct cgatcgcggg ctggaagatg aaaacagcgc gaaagattac
     2821 gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa ttaccggcgt accgcgccag
     2881 tatattgaaa ccatcgcccg cgaatttgcc gataccgccc ataaaacgca tgggcgctca
     2941 atgattatcc tcggcgcagg tgttaaccac tggtatcaca tggacatgaa ctaccgcggg
     3001 atgatcaata tgctgatctt ctgcggttgt gtcgggcaaa gcggtggcgg ctgggcacac
     3061 tatgtcggcc aggaaaaact gcgcccacaa accggctggt tgccactggc ctttgcgctc
     3121 gactggaacc gaccaccgcg ccagatgaac agcacctcgt ttttctacaa tcattccagc
     3181 caatggcgct atgaaaaagt ctctgctcag gagttacttt caccgctcgc cgatgccagt
     3241 aagtacagcg gtcatctgat tgatttcaac gttcgcgccg aacgtatggg ctggctacct
     3301 tctgcgccgc agctggggcg taacccgctc gggattaaag ctgaagccga caaggccgga
     3361 ttatccccca cagaatttac cgcccaggcg ctgaaatcgg gcgatttacg tatggcctgc
     3421 gaacaaccag atagcggcag caatcatccg cgtaatttgt ttgtctggcg ctctaacctg
     3481 cttgactcct ccggcaaagg ccacgagtat atacagaagt atctgctggg gaccgaaagc
     3541 gggattcagg gcgaggaact cggtgccagc gacgggatca aaccggaaga agtcgagtgg
     3601 caaactgcgg cgattgaagg caagctcgac ctgctggtga cgctcgactt ccgcatgtcc
     3661 agtacctgcc tgttctccga tatcgttctg cccaccgcca cctggtatga aaaagacgat
     3721 atgaacacct cggatatgca tccatttatt catccgcttt ctgcggcggt cgatccggcc
     3781 tgggaatcac gcagcgactg ggaaatctac aaaggtattg ccaaagcatt ttcgcaagtg
     3841 tgcgtgggcc atcttggcaa agaaaccgac gtggtattac aaccactgct gcatgactct
     3901 ccggcagagc tctcacagcc gtgtgaagtg ctcgactggc gcaaaggcga atgcgatctg
     3961 atcccgggta aaaccgcgcc gaatattgtg gcggtggagc gcgactaccc tgctacgtat
     4021 gaacgcttta cctcgctcgg gccattgatg gacaaacttg gcaatggcgg taaagggatt
     4081 tcgtggaata cccaggatga aatcgatttc ctcggcaaac tcaattacac caagcgtgat
     4141 ggcccggcac aggggcgtcc gctgattgac accgccattg acgcttcaga agtgattctg
     4201 gcactggcac cggaaaccaa cggtcatgtt gcagtcaaag cgtggcaggc gctgggcgag
     4261 atcaccgggc gcgaacatac ccatctggcg ctgcacaaag aggacgagaa gattcgcttt
     4321 cgcgatattc aggcgcagcc gcgtaaaatt atctccagcc ccacatggtc cggtctggaa
     4381 agcgatcacg tatcctataa cgcgggatac accaacgttc atgagttaat tccgtggcgc
     4441 acgctgtcgg gacgccagca gctctatcag gatcatccgt ggatgcgtgc ttttggtgaa
     4501 agcctggtgg cttatcgccc gcctatcgac acccgtagcg tcagtgagat gcgccagata
     4561 ccgccaaacg gcttccagga aaaagcactt aacttcctga cgccgcacca gaaatggggc
     4621 attcactcaa cctacagtga aaacctgcta atgctgacgc tctctcgcgg tggaccgatt
     4681 gtctggatca gcgaaacaga tgcccgagaa ctgaccattg tcgataacga ctgggtggaa
     4741 gtatttaacg ccaacggcgc gctgactgcc cgcgcggtgg tcagccaacg tgtaccgccg
     4801 ggcatgacca tgatgtatca cgcccaggaa cgcattatga atattcctgg ttcggaagta
     4861 actggcatgc gcggcggcat tcataactcg gtcacccgcg tttgcccgaa accgacgcat
     4921 atgattggcg gttacgcgca gctggcctgg ggctttaact actacggcac cgtcggttcg
     4981 aaccgcgacg aattcatcat gatccgcaag atgaagaacg ttaactggct ggatgatgaa
     5041 ggtcgcgatc aggtacagga ggcgaaaaaa tgaaaatacg ttcgcaagtc ggcatggtgc
     5101 ttaacctcga taaatgtatc ggctgccata cctgttcggt gacctgtaac aacgtctgga
     5161 ccggacgcga aggcatggag tacgcatggt ttaacaacgt cgaaaccaaa ccgggcattg
     5221 gttatccgaa aaactgggaa gatcaggaag agtggcaagg cggctgggtc cgcgatgtga
     5281 atggcaagat ccgtccgcgt ctgggcagca agatgggcgt aataaccaaa atcttcgcca
     5341 acccggtggt gccgcagcag attgatgatt actacgaacc tttcaccttc gactacgaac
     5401 atttgcatag cacaccggaa ggcaaacata tccctactgc tcgcccgcgt tcactgattg
     5461 acggcaaacg gatggacaaa gtgatctggg gcccaaactg ggaagaactg ctgggcggcg
     5521 agtttgaaaa acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag atgtacgggc
     5581 agtttgaaaa caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca
     5641 gctgcgtggc gacctgccca agcggcgcta tctacaaacg cgaagaagac ggcattgtgc
     5701 tgattgatca ggataaatgc cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa
     5761 aaatctactt caactggaaa agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa
     5821 ttgagtcagg ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg
     5881 gcgtgctgct ttacgacgcc gaccgcatcg aggaagcgtc gagcaccgag cgcgaagttg
     5941 acctc
//
LOCUS       DAJTYH010000172         5845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTYH010000172 DAJTYH010000000
VERSION     DAJTYH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..276
                     /locus_tag="OO609_003795"
     CDS             <1..276
                     /locus_tag="OO609_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6635032.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            646..1470
                     /gene="gadX"
                     /locus_tag="OO609_003796"
     CDS             646..1470
                     /gene="gadX"
                     /locus_tag="OO609_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6635033.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1838..2566
                     /gene="gadW"
                     /locus_tag="OO609_003797"
     CDS             1838..2566
                     /gene="gadW"
                     /locus_tag="OO609_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6635034.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2929..5845)
                     /gene="mdtF"
                     /locus_tag="OO609_003798"
                     /pseudo
     CDS             complement(2929..5845)
                     /gene="mdtF"
                     /locus_tag="OO609_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
BASE COUNT         1536 a         1375 c         1381 g         1553 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacac acctgataac ataacgttgt aaaaaccgaa
      301 tgcccagcct ttaaaaaaac agctgggcat tcggttgctt attaatcgca ataatatatt
      361 ggctgtctat tcatcgtgtt gataagatat atgttatatg aatgtttatt aaatgtagcg
      421 attaccttta atgtcacgaa aagggattat ttgcttgcta ttaatttcac tgcgttctta
      481 aacacgtttt tgtattataa atccagtcat cctgcccgta gaacatcaca cattatcatc
      541 ctgttctccc gcttcgttta aatttattta tcaatcaatt tgacttaaga gggcggcgtg
      601 ctacattaat taacagtaat atgtttatgt aatattaagt caactatgca atcattacat
      661 gggaattgtc taattgcgta cgcaagacat aaatatattc tcaccatggt taatggtgaa
      721 tatcgctatt ttaatggcgg tgacctggtt tttgcggatg caagccaaat tcgagtagat
      781 aagtgtgttg aaaattttgt attagtatca agggatacgc tttcattatt tctgccgatg
      841 ctcaaggagg aggcattaaa tcttcatgca cataaaaaaa tttcttcatt actcgttcat
      901 cactgtagca gagatattcc tgtttttcag gaagttgcgc aactatcgca gaataagaat
      961 cttcgctatg cagaaatgct acgtaaaaga gcattaatct ttgcgttgtt atctgttttt
     1021 cttgaggatg agcactttat accgctgctt ttgaacgttt tgcaaccgaa catgcgaaca
     1081 cgagtttgta cggttatcaa taataatatc gcccatgagt ggacactagc ccgaatcgcc
     1141 agcgagctgt tgatgagtcc aagcctgtta aagaaaaaat tgcgcgaaga agagacatca
     1201 tattcacagt tgcttactga gtgtagaatg caacgtgctt tgcaacttat tgttatacat
     1261 ggcttttcaa ttaagcgagt cgcagtgtcc tgtggatatc acagcgtgtc gtatttcatt
     1321 tacgtctttc gaaattatta tgggatgacg cccacagagt atcaggagcg atcggcgcag
     1381 ggattgccga accgtgactc ggcggcaagt attgttgcgc aagggaattt ttacggcact
     1441 aaccgttctg cggaaggaat aagattatag agttttactc agacataaaa aaaacccggc
     1501 ataggggacc gggaagagga tagtctgccg tctccagact aataaaccgt tataacactc
     1561 cctgttggca cgggaaactt tgtgctctca gtaagttaaa tataactttt acaggaaata
     1621 agatcagcca tttttttata aacataagct atacgctgtg cgaaaatata ttcgtgctgc
     1681 atttacttat tatcaattaa ctgttatgca aaactacttt gtggataaat tttggtccta
     1741 ccaaatctgg cagtttttgc gctaagaaac agtctgtcat catttcatta gtatactgaa
     1801 attgaaataa tcgcagtatg aaatataagg gataatcatg actcatgtct gctcggtgat
     1861 cctcattcgt cgttcattcg atatttatca tgaacagcaa aaaatatcgc tgcataacga
     1921 gagtatcctg ctgctggaga aaaatttggc agacgatttt gcgttttgtt caccggatac
     1981 gcgacgactg gatatcgatg agctgacagt ttgccattac ttacaaaata ttcgtcagct
     2041 accacgcaat ttagggttac atagcaaaga ccgtctgtta attaaccagt caccccccat
     2101 gccgctggtg acggcgattt ttgatagctt caatgaatcc ggggtaaatt caccgatact
     2161 gagcaatatg ctctaccttt cctgtttatc gatgttttct cataagaaag aactgatccc
     2221 cttacttttc aatagcatca gcactgtttc aggaaaagtt gaacgcctta ttagctttga
     2281 tatcgccaaa cgttggtatc tgcgcgatat cgcggaaaga atgtatacca gcgagagtct
     2341 catcaaaaaa aagttgcagg atgaaaatac ctgtttcagt aaaatattac tcgcttccag
     2401 gatgtcgatg gccagacgat tactcgagtt acgtcaaatt cctctgcata ctattgcgga
     2461 aaaatgtggc tatagcagta cgtcgtactt tataaacaca tttcgacaat attatggtgt
     2521 aacgccacat cagtttgcgc aacattcgcc aggtaccttt tcctgacata ttttgcattt
     2581 gaatattgat caggatctca cacctgcttc atgtgaaact cttccctgat gatttctgcc
     2641 gggctaccgg ctagttctct ttcgcagttt gccaggcggt ataggtattt ttaggaggaa
     2701 aagactcggc atgtttggga ttattaagct gacaattcat acgattacgg gaatgtgggt
     2761 cagtattgtg ttgttcaaat tgatgacgaa tggctggagt ggtttctact ttcagtgctg
     2821 tgtactaagc ctggtattcc tgacggttag ctggttgttg agtggtgaat ggttagcagg
     2881 aaagagtaag gctgaacctt cacgttcaac cttactctca tttacacgtt acgctttttt
     2941 aaagcgggca aagagatgtt ccactacaac gaaaaagacc ggaacgaagt aaattgccag
     3001 cactgttgcg gcaaacatcc cgcccatcac gccggtacct accgcgtttt gcgcgccaga
     3061 accggcacca tgactgataa ccagcggcag cacgccgaga ataaaggcca gagaggtcat
     3121 caggattggg cgtaaacgca tccgcgccgc ttcgatgatt gcctctatcg gcgttttccc
     3181 ttctttctgc atcatctcaa cggcaaattc gacaatcagg atggcgttct tggcggaaag
     3241 cccgatggtg gtcagcaaac caacctggaa gtagacgtca ttacttaagc cgcgcagatc
     3301 ggtggccagt aatgcgccaa cgacgcctaa cggaacaacc aacatcaccg agaacggaat
     3361 tgaccagctc tcatagagtg cggcgagggc gaggaacacc acgaccagtg aaatggcata
     3421 cagtgcagga gcctggtttg aggataacgc ttcctgatac gacagtccgg tccatgagta
     3481 gccgacgcct gccggaagtt tagcgaccag gtctgccata aatttcatag cgtcaccggt
     3541 acttttcccg gccgccgctt cacctaaaat ctccattgac gggatgccgt tgtagcgttc
     3601 caatcgcggt gaaccatagg tccattcggt agacgagtag gcagaaagcg gtgccatcgt
     3661 gccagaggcg ttgcgtacat accattggtt gatgttatcc ggcaacatac ggaacggcgt
     3721 gcctgcctgg acatacactt ttttcacccg cccctggttg aggaagtcgt tcacgtagct
     3781 gctgccgaag gcggtggaaa ttgtctggtt gatatcagac agcgccacac ccattgcctc
     3841 agctttcgcg gcgttgacgt tcactttgaa catcggcgta tcttccaggc cgttcgggcg
     3901 taccccggtg acctggtcag gcgattgcgc tgccagtgat aacagctcgt ttcgtgccta
     3961 ggttagtttt tcgtgcccca ggttaccgtt gtccagcagt tccatatcaa aacctgacgc
     4021 ggtacccagt tcagccaccg cgggtaagtt gaacgggaag acgacggctt tattgatact
     4081 gcttaacgca atcattgccc gctgaatgat cgcggtaacc gagttttcct caccgacacg
     4141 ttcagaccac ggcttgagac tgataaacgc caggccgttg ttttgcccct ggccgctgaa
     4201 gccaaagccg ccaacggtaa acaccgactg aacattatct ttctcttttg taagataata
     4261 atccgtcacc tgttgcagta ctttcgtggt gttaaccatg gtggcaccgg aaggtaactg
     4321 cgcggtggtc ataaataccc cctgatcctc ttctggtaag aaagaggtcg gtgtacgcag
     4381 gaacagcacc gccatcccgg cgcaaatcag caggtagacc accatgtagc gaccggtaca
     4441 acgcaacagc gagcgggtgc tatcggtata gtgctgagtt gatttttcaa acagcgtgtt
     4501 gaagcgtgcg aacagggcgt taggtttgtg accgccttcc ggcgtggctt tcagaatggt
     4561 ggcgcacagg gcaggggtca ggctcattgc cacaaatact gaaagccgca tggaggagat
     4621 cagcgtgatg gagaactggc ggtagatctc cccggttgca ccgctcataa aggccatcgg
     4681 cataaacact gcagaaagaa caacggcaat cccgaccagc gcacgttgga tctgccccat
     4741 cgatttatgc gtcgcttcct tcggcggtag cttatcttcc gcaatgacac gctcgacgtt
     4801 ctccaccacc acgatggcgt catccaccag taaccctatc gccagcacca tcccgaacat
     4861 agtcaacgtg ttgatggtga aaccgaccgc cgacaagatc gcaaacgtcc cgagaataac
     4921 caccggtacg gcaattgtcg ggatgatggt ggcacggaaa ttctgcaaaa acagataaat
     4981 gaccaggaag acgaggatga tagcctcaac cagtgttttg aaaacttcct gaatagaaat
     5041 ttcgataaac ggcgtggtgt cgtaaggata aaccgtcttc agacttgcgg ggaaataggc
     5101 tgataagcgg ttcagctcct ctttgactgc ccgcgaggta tccagggcgt ttgctccggc
     5161 agccagtttg atggcgatcc cggcaacagg tttgccgtta tagcgcgcca cggtggaata
     5221 atcttccgcc ccaagttcga cgcgggcgac atcacgcagc agcacttgcg aaccatcttg
     5281 ctgaactttc aacaggattt tgccaaattc ttccggcgtt tgcagacgcg tctgcacaat
     5341 gatcgaggcg tttagttgct ggtctgccgc ctgtggcatg ccacccagtt gaccaccaga
     5401 aatctggttg ttttgcacct taatctggga aataacatcg gaaggtacca ggttatattt
     5461 attgagtttt tgcgggtcca gccagatacg catggcgtac tcggaaccaa agagctgtac
     5521 gctaccaacg cccgcggtac ggcttagcgg gtctttgata ttagacgcta catagtccgc
     5581 gatatcgtac tggttgaggc tgccgttatc agaaataaac gccgctacca tcaggatatt
     5641 actgctcgac ttatcgacgc taatcccctg ttgctgcacc gcttcaggta atgaaggcat
     5701 agcgagttgc agtttatttt gcacctgaac ctgtgcgata tcaggagatg ccccagtctc
     5761 gaaggtcaga gtgatagagg cattacccgc cgcatcactg gttgaagaca tgtacatcag
     5821 gccatcaagc ccattcatat tttgc
//
LOCUS       DAJTYH010000173         5839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTYH010000173 DAJTYH010000000
VERSION     DAJTYH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            420..1328
                     /locus_tag="OO609_003799"
     CDS             420..1328
                     /locus_tag="OO609_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6635035.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1374..3365)
                     /gene="tkt"
                     /locus_tag="OO609_003800"
     CDS             complement(1374..3365)
                     /gene="tkt"
                     /locus_tag="OO609_003800"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6635036.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3643..4401
                     /gene="loiP"
                     /locus_tag="OO609_003801"
                     /gene_synonym="yggG"
     CDS             3643..4401
                     /gene="loiP"
                     /locus_tag="OO609_003801"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6635037.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4607..5527)
                     /gene="speB"
                     /locus_tag="OO609_003802"
     CDS             complement(4607..5527)
                     /gene="speB"
                     /locus_tag="OO609_003802"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6635038.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5663..>5839)
                     /locus_tag="OO609_003803"
     CDS             complement(5663..>5839)
                     /locus_tag="OO609_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635039.1"
                     /translation="NVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCSGQAF
                     PADNNGYAPPPCRK"
BASE COUNT         1434 a         1526 c         1519 g         1360 t
ORIGIN      
        1 aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc cttattttat
       61 ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg aaagcaattg
      121 ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata acaacaatat
      181 gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa atcaacattt
      241 tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt cgaaaacata
      301 cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt ctcacctgtc
      361 ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg aagattgtca
      421 tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct atcgccattg
      481 gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat gaaggtgaac
      541 gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg ctgggtttta
      601 agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct gtggtctatc
      661 agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta tcagtcagct
      721 tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact attgaagcat
      781 taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt ttcggccaat
      841 ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg caaaatgcca
      901 tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa aatattgatt
      961 tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc gccattgcca
     1021 cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta actcaggccc
     1081 aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact attcgcgtaa
     1141 gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca gaagcaatcc
     1201 gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc actgccgaac
     1261 gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg tttatctcta
     1321 ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac tgattacagc
     1381 agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc aaacagcagc
     1441 tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt caggccaaca
     1501 tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc
     1561 ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg
     1621 gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc aaccgccagt
     1681 tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca gtctttcagc
     1741 acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg ctgcgccagg
     1801 ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac
     1861 gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc
     1921 agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag accgatggag
     1981 tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg tacggcgtta
     2041 cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg
     2101 gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg gatgtagtta
     2161 cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt agacggtgcc
     2221 aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc gatagcattc
     2281 tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc gataaactct
     2341 ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt aaattcagcc
     2401 gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc agattctttc
     2461 gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat ttcgaacggt
     2521 gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca
     2581 ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat ggttttgcac
     2641 atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg tttgatggat
     2701 gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt
     2761 gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc gttgtcatcg
     2821 tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac ttcgtgggag
     2881 atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg
     2941 tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat accgactgcg
     3001 ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt ataacctact
     3061 tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc ttccatcggc
     3121 agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg gccgttggac
     3181 agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt caggaaatca
     3241 cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg accggatttg
     3301 gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc tttacgtgag
     3361 gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg acgacagcgc
     3421 gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg tcatgtacat
     3481 ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc
     3541 tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac ctgcaaacgg
     3601 cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta
     3661 ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga ctccaacgga
     3721 ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa
     3781 accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc gccagccaat
     3841 agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa tatcaacggt
     3901 cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc aatggctaac
     3961 ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga agtcgaagcg
     4021 gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg
     4081 gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat tgtcggcagt
     4141 ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca attctcccag
     4201 cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg cggcatcagc
     4261 ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg tcgtcaaagc
     4321 tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc
     4381 agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat aaggcgttca
     4441 cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg atgcgatgct
     4501 ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt tcacgccgca
     4561 tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt
     4621 tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga gcggtgattt
     4681 ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg ttgagatctt
     4741 tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc actggcgtac
     4801 cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg taaaccggca
     4861 tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga tcgttcacct
     4921 ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga ataccaatct
     4981 gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac atagtgccgt
     5041 ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt accagcgcca
     5101 ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag tggtcaccac
     5161 cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt
     5221 cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc acgacgttca
     5281 gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc agattcgtcg
     5341 aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa gtggccatat
     5401 cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg aagttcatcg
     5461 gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga tgacctaagg
     5521 tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg cgtcgttagg
     5581 cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc gccttaaatg
     5641 ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag ccattgttat
     5701 ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt
     5761 caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc gaattggctg
     5821 cgtaggaatc agcaacgtt
//
LOCUS       DAJTYH010000174         5657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTYH010000174 DAJTYH010000000
VERSION     DAJTYH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            5..343
                     /gene="spa13"
                     /locus_tag="OO609_003804"
                     /gene_synonym="spaM"
     CDS             5..343
                     /gene="spa13"
                     /locus_tag="OO609_003804"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6635040.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            330..1208
                     /gene="spa32"
                     /locus_tag="OO609_003805"
     CDS             330..1208
                     /gene="spa32"
                     /locus_tag="OO609_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6635041.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            1202..2083
                     /gene="spa33"
                     /locus_tag="OO609_003806"
                     /gene_synonym="spaO"
     CDS             1202..2083
                     /gene="spa33"
                     /locus_tag="OO609_003806"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6635042.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            2088..2738
                     /gene="spa24"
                     /locus_tag="OO609_003807"
                     /gene_synonym="spaP"
     CDS             2088..2738
                     /gene="spa24"
                     /locus_tag="OO609_003807"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6635043.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            2753..3013
                     /gene="spaQ"
                     /locus_tag="OO609_003808"
     CDS             2753..3013
                     /gene="spaQ"
                     /locus_tag="OO609_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6635044.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            3013..3783
                     /gene="spa29"
                     /locus_tag="OO609_003809"
                     /gene_synonym="spaR"
     CDS             3013..3783
                     /gene="spa29"
                     /locus_tag="OO609_003809"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6635045.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            3790..4818
                     /gene="spa40"
                     /locus_tag="OO609_003810"
                     /gene_synonym="spaS"
     CDS             3790..4818
                     /gene="spa40"
                     /locus_tag="OO609_003810"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6635046.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            4831..5073
                     /locus_tag="OO609_003811"
     CDS             4831..5073
                     /locus_tag="OO609_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635047.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         1805 a          803 c         1042 g         2007 t
ORIGIN      
        1 agatgtggag gcattagata aaaggattat atacttcctg caactggaga atgacttaga
       61 acctgtaggc gctcagagtg ttagtcagct tttcaataca agacgtaaga tcgctatagt
      121 aaaaaagcat attatccaat atcagtctga acgaattctt ctaaagggaa gaattgaaga
      181 aattcaaaaa gatattgatg aggcaaatgc ttctaaaaga aaactgcttc ataaagagag
      241 taaaatatgt aaacggattg gcttgattaa aagaaataac tttgccaaac agttgatact
      301 tgatgagtta tcacaagagg atatgaagta tggcattaga taatataaac ctaaatttta
      361 gtagtgacaa acagatagaa aaatgtgaga aactatcttc aatagataat attgatagtc
      421 tcgttttgaa gaagaagagg aaggttgaaa ttcctgagta ctctttaatt gcatctaact
      481 attttactat tgataagcac tttgaacata agcatgataa aggagaaatt tatagtggca
      541 ttaaaaatgc gttcgaactt agaaacgaac gagcgacata ttctgatatt ccagaatcaa
      601 tggccattaa agaaaatatt ttgataccag atcaagatat caaagcaagg gaaaaaataa
      661 atatcggcga tatgaggggg atcttttcat ataataagag tggaaatgca gacaagaact
      721 tcgaaagaag tcatacttct tctgtaaacc ctgataatct gctagaatct gataatagaa
      781 atggtcaaat tggtttaaaa aatcatagct tgtctattga taagaatatt gctgacatca
      841 tttctttact aaatggaagt gttgctaaat catttgagct gcctgtaatg aataaaaata
      901 ctgcagacat aaccccatcc atgtcattgc aagaaaaatc aatagttgaa aatgataaaa
      961 atgtttttca aaaaaatagt gaaatgactt accactttaa acagtggggg gctggacatt
     1021 ctgttagtat ttcagtggag tctggttctt ttgttctaaa accgtcagat caatttgtag
     1081 gaaataaact tgacttaatt ttgaaacaag atgctgaggg taattacaga tttgatagca
     1141 gtcaacataa taaggggaat aaaaataata gtacaggata taatgaacag agtgaagaag
     1201 aatgctaaga attaaacatt ttgacgctaa cgaaaaacta cagattttat atgcaaagca
     1261 actctgcgag cgtttttcga ttcagacatt caaaaataaa tttacaggca gtgaaagttt
     1321 agtcactctt acttccgtgt gtggggattg ggtaattcgt attgatacat tatctttttt
     1381 gaaaaaaaaa tacgaggtat tttcaggatt ttctacacaa gaatctttac tgcatttatc
     1441 aaaatgtgtc tttatagagt cgtcatctgt attttcgatt ccagaactgt ctgataagat
     1501 tactttccgg atcacgaatg aaatccagta tgcaactact gggagtcatt tatgctgttt
     1561 ttcctcttct ttaggtatta tttattttga caagatgccg gtattacgta atcaagtttc
     1621 tcttgactca ttgcatcatc ttttagagtt ttgcttaggt tcatctaatg taaggctggc
     1681 tactttaaaa agaattcgca ctggtgatat aatcatagtt cagaaacttt ataatttatt
     1741 attgtgtaat caagttatta ttggggatta tattgtgaat gataataatg aggcaaaaat
     1801 taatctgtca gaaagtaatg gtgagtcaga acacacagaa gtttctttgg cattattcaa
     1861 ttatgatgat atcaatgtaa aagtggactt tattctttta gaaaaaaata tgacaatcaa
     1921 tgaactaaaa atgtatgtag aaaacgaatt attcaagttt cccgatgaca tagttaaaca
     1981 tgtaaatatt aaagtaaatg gttctttggt tgggcatggg gaacttgttt ctattgagga
     2041 tggttatggt atcgagatta gttcttggat ggtaaaggag taatctcatg ctgagtgaca
     2101 tgtccctcat cgctacactt tcttttttta cgctattacc atttttagtt gctgctggta
     2161 cctgttatat aaagttttct attgtttttg taatggtacg aaatgcactt ggtttgcagc
     2221 aggttccatc aaatatgact ctcaatggta ttgcattgat tatggctttg ttcgtgatga
     2281 aacccattat tgaagctggt tatgaaaatt atctaaatgg ccctcaaaaa tttgatacaa
     2341 tatctgatat tgtccgtttt tctgattctg gcttgatgga atataagcaa tacttgaaaa
     2401 agcatacaga tcttgagtta gcacgttttt tccaaaggtc tgaagaagaa aatgctgatt
     2461 taaagagtgc agagaataat gattattcat tgttttccct cttgcctgca tatgcgctaa
     2521 gtgaaatcaa agatgcgttc aaaataggtt tttatttata tttgccattt gttgttgttg
     2581 atcttgttat ttcaagcatt ttacttgcac ttggtatgat gatgatgagt ccaatcacaa
     2641 tatcagtacc aataaaactt gtattatttg ttgcgcttga tgggtggggt atcctgtcta
     2701 aggccttgat tgaacaatat atcaatattc ctgcttagat gatggaggta atgtgtctga
     2761 tatagtttat atgggtaata aggctcttta tttaatcctt atcttttcct tatggccagt
     2821 aggtatagct acggttattg gattatctat tggtttgtta cagacagtga ctcaactcca
     2881 agagcagaca cttccttttg gtataaagct tataggtgtc tcaatatctt tgctacttct
     2941 ttctggatgg tatggtgagg ttttattgtc tttttgtcat gaaataatgt ttttaattaa
     3001 gagtggggtt tgatggacat ttcaagctgg ttcgaaagta ttcatgtgtt tttaatactc
     3061 ctgaacggcg ttttttttag attggctcca ttatttttct ttcttccatt tttaaataac
     3121 ggtataattt ctccatctat tagaatacct gtgatttttc ttgttgcatc aggattaatt
     3181 acttctggta aggtagacat aggttcttct gtttttgaac atgtttattt ccttatgttc
     3241 aaggaaataa ttgttggcct ccttctctct ttttgcttgt ctcttccctt ttggatattt
     3301 catgctgttg gtagcattat tgacaaccag cgtggggcaa cgcttagtag ttcaattgat
     3361 cctgccaatg gtgttgatac gtctgagttg gcaaaatttt tcaatctttt ttctgcagtt
     3421 gtatttctat acagtggtgg tatggtcttt attttagaat ccatacaatt gtcttataat
     3481 atatgcccgt tattttctca atgttctttc cgtgtctcaa atatcttaac atttctgact
     3541 ttattggcaa gtcaggctgt tattttagcc agtcctgtta tgatagtatt gttactatca
     3601 gaagtattac ttggtgtatt atcgagattt gctccgcaga tgaatgcttt ttccgtatca
     3661 ttaactatta aaagtttact tgcaatattt attattttca tctgttcttc tactatttac
     3721 ttttctaaag ttcaattttt cctcggtgaa cataaatttt tcacaaatct atttgttaga
     3781 taaaatatta tggcaaataa aacagaaaag ccgacaccta aaaaactaaa ggatgccgca
     3841 aaaaaaggac agtcatttaa atttaaggat ttaacgactg ttgttattat tctggtaggg
     3901 acatttacta taatatcatt cttttcctta agtgatgtaa tgctcttata cagatatgta
     3961 ataattaatg acttcgaaat taatgagggt aaatactttt ttgcagtggt tattgtcttt
     4021 tttaagataa ttggcttccc actttttttc tgtgttcttt cggctgtgtt gccaacattg
     4081 gttcaaacaa agtttgttct tgcgactaaa gctatcaaga ttgatttttc agtattaaac
     4141 cctgttaaag ggttaaaaaa aatatttagt ataaagacaa taaaagaatt tttcaaaagc
     4201 attctgcttc ttattattct agcattaaca acctatttct tttggattaa tgaccgaaaa
     4261 ataatttttt ctcaggtgtt ttctagtgtt gatggcttat atcttatttg ggggaggctg
     4321 tttaaggata taatattatt tttcttggca ttttctattc ttgttattat tcttgacttt
     4381 gtgattgagt tcattttata catgaaagat atgatgatgg ataaacagga gataaaaaga
     4441 gaatatatag agcaagaggg acactttgag acaaagtcga gaaggcgtga gttgcatatc
     4501 gagattcttt cagagcagac taaatctgat atacgtaatt caaaattagt ggtaatgaac
     4561 ccgactcata ttgcaattgg tatttatttt aatccagaaa tagcgcctgc accttttatt
     4621 tctctcattg aaactaacca gtgtgccttg gctgtcagaa aatatgcaaa tgaagttggt
     4681 ataccgactg tgcgtgatgt gaaattagct agaaaactat ataaaacaca tacaaaatat
     4741 agttttgttg attttgaaca cttggatgaa gtcctacgtc ttattgtttg gcttgagcag
     4801 gttgaaaaca ctcattaata agtaaaggag atgtgttata tgggagttaa tttctgtaat
     4861 aaaataggta ttgatcagtc tgaatttgaa atagaatctt ccatcattaa ctccattgct
     4921 aatgaggtat tgaacccaat atctttcctt agcaataagg atataataaa tgttttactc
     4981 agaaagattt catctgaatg tgacctggtt agaaaagaca tttatcgttg tgctctggag
     5041 ttggtcgttg aaaaaacacc tgatgattta taatcattca taataactct tttgctgata
     5101 taaatatttt tgttgaagat atttatgtag tcacaggatc aattgtagaa aattgttatg
     5161 tttttgttag gaactgcact aaataatata tgataagaca gcaaaaacga ctgaccataa
     5221 tccttctttt attaggagtt gataagagag actattcatc ctgtaatgtt aaaactctgt
     5281 tatatagtat tcgcgattat gcaaagtctg taaatgatca tgagatacta accgagagta
     5341 atcgtttatt atctcattgc atttcagata gtaacggagc tttctttaaa agtagtaaat
     5401 acgttcccct taagtactta cgtaaaagga gaatagcacg aaagatacca aatgattagc
     5461 actttgctag taagcttgct ggtaaactat attgaccgtt gggtttgttt tgagactgta
     5521 atttaaattg tgtaattgcc tgtttttgat atgtttactc caacaacgga gacaggtaaa
     5581 ttatggacga aaagaagctt aaagcccttg cggctgaact ggctaaaggc ctcaaaactg
     5641 aggccgacct caatcag
//
LOCUS       DAJTYH010000175         5551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTYH010000175 DAJTYH010000000
VERSION     DAJTYH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            515..1693
                     /gene="ltrA"
                     /locus_tag="OO609_003812"
     CDS             515..1693
                     /gene="ltrA"
                     /locus_tag="OO609_003812"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6635048.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1776..1955
                     /locus_tag="OO609_003813"
                     /pseudo
     CDS             1776..1955
                     /locus_tag="OO609_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1985..2467)
                     /locus_tag="OO609_003814"
                     /pseudo
     CDS             complement(1985..2467)
                     /locus_tag="OO609_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2723..3703)
                     /locus_tag="OO609_003815"
     CDS             complement(2723..3703)
                     /locus_tag="OO609_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6635049.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3703..4902)
                     /locus_tag="OO609_003816"
     CDS             complement(3703..4902)
                     /locus_tag="OO609_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6635050.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5224..>5551)
                     /locus_tag="OO609_003817"
     CDS             complement(5224..>5551)
                     /locus_tag="OO609_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6635051.1"
                     /translation="WYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTSDDW
                     GSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIGSFI
                     EKHMFY"
BASE COUNT         1533 a         1266 c         1310 g         1442 t
ORIGIN      
        1 ttatccatgc tctgaggtgg gtagaggtgg gccctccggt cagggtttac aggagcacag
       61 accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac tcgggaaaag
      121 gcagagatct actgtcacgt gggttcggga agctgaacga aagtaaactg tccgatgaag
      181 catcgaaatc ttagatgatg ccaaaatggg gccgtacagc cgtcccctga taagcgcaga
      241 agacacctgc ttactgactg cgcggcatcc ggtgtgaagg cagcaggatc ccgggccaga
      301 ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc gggaaacagg ggcggaccca
      361 ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg aagggatggg gatcacattc
      421 attgcagaag acacaaccca aaaggggatg acgtaactga tgcaacgaaa atcatttgaa
      481 ataccaaaag cactggtctg ggcatcatat ctggatgtgc gtcgcaacaa gggagccccc
      541 cggatgcgac gggcagactt tgaaaatgtt cgaccagcaa cgtgatggca atctatacaa
      601 gatctggaac cggctatgtt cgggaacatg gtttccgcca ccagtgctgg aaaaacggat
      661 cccgaagtcg aatggcaagg aacgcattct ggggatccca acagtatccg atcgaattgc
      721 tcagggagcg ataaaacttt tcatggaaga aaagcttgac ccgattttcc atgctgattc
      781 atatggttac cgaccaggca aatcagctca tgacgctctg aaacaatgtg ccatccggtg
      841 ctggcgttat agctggatac tggaagtgga catcagtgcg tttttcgacc atgtgagaca
      901 tgatctggtg ctcaaagctc tggaacacca cgggatgcct aaatgggtca tcctgtactg
      961 tcgacgctgg atggaagcgc caatgcagag ttgtgaaaat ggagaattaa taacccgaac
     1021 acgaggcact ccgcagggcg gggtcattag tccgctactt gctaacttgt tccttcacta
     1081 tgcatttgat ttgtggatgg aaagagaata tcggggggta ccgtttgaga ggtacgctga
     1141 cgatattgta gtacattgtt cacgaatgag tgatgcaaca aggctaaaga acaggttatc
     1201 ggagcgcttc tcggaagtcg ggctggtcct gaatgcaggg aaaacgaata tcgcctacat
     1261 tgacacgttt aaaaggagaa acgtcgcaac gagtttcacc tttctcggat atgacttcaa
     1321 agtgcgtacg ctgaagaatt tcaacggcga acggtaccga aaatgcatgc cgggtgcgtc
     1381 aaatgcagca atgcgcaaaa taacagaaac aatcaagaag tggcgtatac atcgctcaac
     1441 agctgagagt ttgctggatt ttgcgaggcg ctacaatgcg atagtgagag gctggatcga
     1501 gtactacgga aagttctggt ccagaaattt caactatcga ctgtggagtg caatgcagtc
     1561 acgtctgctc aagtggatgc agtctaaata cagactttcg aaccggaagg ctcagcgaaa
     1621 gctgacgctg gtaaggaagg agtatccgaa gctatttgtt cactggtatt tactacgtgc
     1681 atcaaatgag tgatcaagag ccgtatgacg cgagagtgtc acgtacggtt ccgtgagcag
     1741 ccggggagtg aaattctctc cggctgactc gactcgcggg ttccgacagc gatgacgaac
     1801 atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg gaaccagaaa
     1861 aatggctgcg ttacgtcatt gagcatatcc aggactggcc ggcaaaccgg gtacgtgatc
     1921 tgttgccctg gaaagttgat ctgagctctc agtaaatatc aatacggttc tgacgagccg
     1981 cttacttttg gcgtaattct gctgcgcctt tactgaacac aatgccgtat tcagcaagaa
     2041 gtccacgaat gtgatttatc agttgtgtcc tttccattga aaccaaccga cgcatattca
     2101 aaaaagtctg gacgtcctgt tgctccagag tttttattgg tacgggtcgt atagttccat
     2161 gttggcaggc ctcagctatt gcctgtgcgt cgttataatc atttttctgg caccgctgat
     2221 aagctttaac atgttgtgcc ggaagcaata ttacatcatg gtcgagtttc ctgatttccc
     2281 gggcccagta atgagatgcc ccgccagcct ccatcgcaac agtcatttta ggctgttgca
     2341 ccagccagtt aagcagttga ttgcgagaaa gtttggtctt tcctgcgggt ttgccatgag
     2401 cattgatact gaaaagataa aagttcgttt tagccagatc aatgccggtg attttctgtt
     2461 cagacatggt atcccgctta cttttgttgt taaagtttat ccatcatggc gcaattgaag
     2521 gctgccgtga aagaggggga agactatctc atcggttact gttaaacggc taagagaaac
     2581 tatctttttc aagcaaagaa gatacggtga aacaggcgat ttcaccgtga aatactgttc
     2641 aaaaaaaacg ctaataaaag caaaatttct tttaaaacag ccaattaaaa actggcgagt
     2701 tgctggcgaa aaacttgaat tttcacagcg aattcagatt atcttttagt acaagagcaa
     2761 tcatacgatc taacttatcc tgaacttcca acggtaatct gccgaattca tatgagaacg
     2821 tacgtccctt tactcgtttt cgagcaaatt tatctttact atcaaatttc cataacaaag
     2881 ttacaaccgc tttatccttt actcctgaat caagcaatga agacatttcc ttggttataa
     2941 gccgcataat actgttttta acctcgtctt cagaaagatt gtcatcagca gtaataagct
     3001 caatactcgg cgagatattc ttcacaactt catcaataga tatatcaccc agcctcagtc
     3061 gttcacttaa ttcagccagt tgcctgtaat cggcgtatgt cagctcagac tgaacaggaa
     3121 aaagagacac aaaatcttta ggtacggacg ctgcttggag ggcacgagtc accttagctg
     3181 ccgacaatcc ctctctttcc gcaatttgct tctggttcat accggcctct ttgagacgta
     3241 ctaaacgcaa cccaatttca cgaatattat gttcaagtga cgtctgcaaa tctttcgcca
     3301 gatgctgagc ttcagaaaca gtaagctcat catccgttac aagtacacgt aatccaacct
     3361 tgcatagcaa agctgctgcc cgacgacggg aaccatccaa aatctcaata agatcgccag
     3421 tccgaatccc aatacatgga tagaattgtt gtaaacgtat agttctggtg atgtctttta
     3481 gtgagtccgg cgtcagggct gtctgttcgc ggccattcac ctcttcaacc acaaaagttt
     3541 gagtatccac tttgtcatga tcaaccttga tctcagtaaa ttttgccttt cggccagtat
     3601 tgagggtaaa tacatgaact ggagccgata tttcttcagt attattcagg atatttgtat
     3661 tcagcgtacg gccaatagtc ggtcggtgct ttctgttttc catcagtagt tggcccttat
     3721 aaattcaata cgatcaaaaa ctgctttagc gaaatcttct gctgccatac gtgcattttt
     3781 caaagcttca ccactgccaa cataagtaac aggattcgca gatatcacgg tatcaaacga
     3841 ctcgccgctt ctctcaaaac catcaagacg tggcattgaa acatcaagca tgtcaccacc
     3901 gaaaatctct tttgtcaggc tatggcagta tttatgatct gacttattcg ccagtttcga
     3961 catgaagcca atatttgcct gcaaacggca tgaacatcca tcctgttcaa taatctgtac
     4021 aagttcaggt aaacgagcca aatatttaag cgtggaatgg aaatcaactt gtgctggcgg
     4081 caccggtgta aacatgatat ctgcggcagc aatggcattt ttcaaaaatg cgtccagatg
     4141 cgggcctgta tcaataagga tgaagtcgaa atcatgcttc agcttatcaa taatattctc
     4201 acgcaatatc gcgtgtttgt tttgtcctaa tagatgctcc tcgcacagag tatcccagtt
     4261 tgatgcaata aaggcgtcgt caatagaagc cggcataaca taaacgccag ggataactga
     4321 cggaacgata aaatcttcca gtaattcctc acgactcacg ttctgcaaca tagcctgcgg
     4381 tgcggttgta tcaaccagcc ccaccgcatg caagtagttc agaaacatag tggctgatga
     4441 ctggggatct aaatcaagga gcagaatacg taaatcttct gacagcaggt gaggatgagc
     4501 acgcagggcg tgagctacgg aaacactact cacggtttta gacacaccgc cttttagact
     4561 accgatgaat atggaatatg cctcactata acgatcgcga tatttaggaa tgccacgatg
     4621 tgcatagata tcgataatgt tctggatggt taatgcatat tttgtcgcgg ttcctgctgg
     4681 gcgtttttca aactgatagc cctgagcctc catctcaccg actgcaccat ccacgcttgc
     4741 gcgcgttaat tttggaagct tagctacagc agactttgaa tagacttgat aaaaaacatc
     4801 agcctgaaac tcctgctttt gctcctgaat ctgttttgtt agggcgagca acattttatc
     4861 tgcacgctgc gcaaccttac ttaactgctc aaaactagtc attccccctc cgtgctatgt
     4921 aggattttaa cttattacaa aaaaaactgc attagcaggg taatgcatgc aagtacatat
     4981 tgtacatgct tatattaaaa atgcgcgcat tgctgattta ttaatcatcg gaatgaatat
     5041 tactgtaaga tagtatgcct ttccgggaga aagacgctga tttcccccga ataatccagt
     5101 tcaccaccac actccggaca acagctttct tcaggctcca gatggtgtat ttcgcgggaa
     5161 acatgttccg gtaacggacg gcgatgacgt gactgtcgca acaggcgggg gtagtgcttc
     5221 caatcagtag aacatgtgct tttcaatgaa ggagccgatg actttttcgt ggatttccac
     5281 ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc ggagggtccg
     5341 gttgttacgt gcaatacgtt gcgtgaagat tttgccggtc aggtgtttct ccttcggcac
     5401 ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga acggagtcaa
     5461 cagggctaac agttcccgac aggtctcatc attccgtggc ccgaaagtgt aagccaacac
     5521 accgccagtt ttagtgttgt acgcatacca g
//
LOCUS       DAJTYH010000176         5519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTYH010000176 DAJTYH010000000
VERSION     DAJTYH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..299
                     /gene="mddA"
                     /locus_tag="OO609_003818"
     CDS             <1..299
                     /gene="mddA"
                     /locus_tag="OO609_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6635052.1"
                     /translation="DGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVA
                     GIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQLQLDERTEPDAIG"
     gene            296..745
                     /locus_tag="OO609_003819"
     CDS             296..745
                     /locus_tag="OO609_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6635053.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(746..979)
                     /locus_tag="OO609_003820"
     CDS             complement(746..979)
                     /locus_tag="OO609_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6635054.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1065..1238)
                     /gene="ortT"
                     /locus_tag="OO609_003821"
     CDS             complement(1065..1238)
                     /gene="ortT"
                     /locus_tag="OO609_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6635055.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1433..1528
                     /gene="yncL"
                     /locus_tag="OO609_003822"
     CDS             1433..1528
                     /gene="yncL"
                     /locus_tag="OO609_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6635056.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1930..2739)
                     /locus_tag="OO609_003823"
     CDS             complement(1930..2739)
                     /locus_tag="OO609_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6635057.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(2949..4373)
                     /gene="patD"
                     /locus_tag="OO609_003824"
     CDS             complement(2949..4373)
                     /gene="patD"
                     /locus_tag="OO609_003824"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6635058.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4395..5189)
                     /locus_tag="OO609_003825"
     CDS             complement(4395..5189)
                     /locus_tag="OO609_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6635059.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5179..>5519)
                     /locus_tag="OO609_003826"
     CDS             complement(5179..>5519)
                     /locus_tag="OO609_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6635060.1"
                     /translation="PVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAAGS
                     IFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYLAF
                     VKRLGAFDAL"
BASE COUNT         1457 a         1414 c         1207 g         1441 t
ORIGIN      
        1 tcgatggttt tcgccatacc gtggaacatt cggtttatgt ccatcccgat catcagggca
       61 aaggtctggg gcgtaaattg ttaagtcgat tgattgatga agcgcgggat tgcgggaagc
      121 atgtcatggt cgccgggatc gaatcgcaaa atcaggcttc gctgcatctg caccagtcgc
      181 tgggatttgt cgtcaccgca caaatgccgc aggtaggcac taaatttggt cattggctgg
      241 atctgacatt tatgcagttg caactcgacg agcgcactga accggacgcg attggatgaa
      301 tcagtcgctc acccttgctt ttttgattgc cgccggaatt ggtctggtag tccagaacac
      361 gctgatggcg cgcatcaccc agacctcctc taccattctc atcgccatgt tgctgaactc
      421 actggtgggg attgtgctct ttgtttctat tttatggttt aaacaaggca tggcggggtt
      481 tggcgaactg gtgtccagcg tgcgctggtg gacacttata cctggcttgc tgggttcgtt
      541 ttttgtcttc gccagtatca gcggatatca gaatgtggga gcggcgacaa ctattgcggt
      601 actggtcgcc agccagctga ttggcggtct ggtgctggat atcttccgca gtcatggcgt
      661 accactacgg gcgttgttcg gcccaatatg tggcgcaatt ttacttgtgg ttggcgcctg
      721 gttggtggcc agacgctcat tttaatcaga ggatggtgcc gcctttggtt aactgttcgt
      781 ggcgcgcttc catatcttct ttatgcttgc gaccgtgatg agcaatagcg gtgcgcaaac
      841 gctgctgctg ggtgtagcga tcttcccgac ttaactccgc gtcatcgctc agggcgatta
      901 ataattcgtt catatgggca atgacgctct cttcaatcgc ggcatccacg cgcgcgatga
      961 cttcatccag atgcgacata ctttctcctt agccacttgt gaaatttatc gattaatcca
     1021 aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta agatttaaaa cagtgaaaac
     1081 aacagcgcca ccgggaaact catcggccat gtggccccca ccagaaaggc gcttaagaaa
     1141 cggatgcgtt tcttatcgtg agaaagaaac caggtgataa gaaatgcgat tgctgccata
     1201 accgcataaa aaacaagcat atgttgatag agaaacatgt acgggcgtcc agccatatga
     1261 ataaaacgcg cgcaaatatg cgccattttt tgacgtgcat caagtttttt caccagtttg
     1321 agaaaaaatc atcaaacagc aacttgccct tatccatctt cagaatgagt attattgcgg
     1381 attttcttaa cccgtactat acacataggg gcaatgataa aaggtggcaa aaatgaatgt
     1441 ttccagtaga actgtagtac tgataaatgt ctttgctgct gttggtttgt ttactcttat
     1501 ctctatgaga tttggctggt ttatttgatg tagtgatttt gcacgcagca cgtactgtca
     1561 gagaaatcaa ctctctgaca gtacgcaaga tcttctcata ctctccaagt ttgcaacatt
     1621 atccttaata tttacttctt tttgctggca tctacattga aatgagtaga aaacatcccc
     1681 ctactgtcgt ctatacaaac agcttactga ttcaaacgga atcttgtttt cacatttttc
     1741 tatcacgaat actttatacg cgcgggagtc tataaatgct ttatactttc aaggaataac
     1801 gtaaatataa aaagatggcg taaagtatat gcatggcagg atgtttgcaa cctccttgat
     1861 gacgctgccc cattgaaatt gcaaaccccg ccctggttat cttgcccatt tatcatgcaa
     1921 aacagataat tataggcctc tgaatacctc agaaagattg aattttttat agtccctctc
     1981 ttgaatattt ccatttgata tcaatcgatt catctcttca acaaaataat ttatatcatc
     2041 acacttatta ttatccggaa gatctgagta gttataattc caccatttta ttctcaacag
     2101 ttcatcaata acatttgaat caaacctaaa tctgattatt tttgcaggaa ctcctgcaac
     2161 tatggcatat gggggcacat ctttggtaac aacggaattg gcagcaataa cagcaccatc
     2221 acctattgcg atgccacctt tcaatacaac atcatttcct atccagacat catttcctat
     2281 aatcggattt tcaggtttgg taatgaatgg tttcaatgtc cagctacgtt caaattcact
     2341 catagcaaac ttatcaaatt caccgttata agtcatcatg tgagtagtaa aacgattcaa
     2401 aggatgattt ccacccatta tttttacatt tgatgcaata ctacaaaatc gacctatacg
     2461 tatattccct gaaaaatggc aggtcggaaa tgaaaaagcg ccgacactgg agaaattttt
     2521 cacaggcatt gttgaatatt cttcagcaat aacattatct tcaattgtta tcttgtcacc
     2581 ttttgagaaa agaaataaat tcctttcttt atacgacttc ttccccttat gatttaaaaa
     2641 gattttatta tccatacaaa actcaaagtg ttttttcgtc catctgaagg taagggtttt
     2701 atttttatct tccgtgaatt tgtacaattg aactttcatt gttaatactt ctacttataa
     2761 agtattagtt taataacgat actctaaagc cttatgctaa tcaactgtga caggatgaaa
     2821 aatcgccatc aaacatttag cctaattttt acgccagtta ttaatgtaat cattctaata
     2881 cttaaccgcc attaaactca gaacctaaaa cgatagtggc cgtcctataa cagccactaa
     2941 catctaactt aatgtttaac catgacgtgg cggacgacgg tgtaatcctc cagcccataa
     3001 agtgacatat ccttgccgta accagaaagt ttctgcccac cgtgcggcat ttcacttacc
     3061 agcatgaaat gggtattgac ccaggtacaa ccatattgca gccgtgcgct gacgcgatgc
     3121 gccctgccca catctttcgt ccataccgaa gatgcaagtc cgtactggct gtcattcgcc
     3181 cagttcacca cctgttcttc gttgtcgaag ggcgtaacac tcactactgg accaaatacc
     3241 tctttttgca cgatggcatc gtcctgtaat gcgccagcca gcagcgtcgg cgcatagtaa
     3301 tagccattac ccttgcgctt ttcaccgcca gtgatcactt tgatgtgccc tgtcgctttc
     3361 gcctcttcta ctgccttgct gacgcgttcg agatgcgcca gcgagcttaa aggtccaagc
     3421 tccgtagact cgtcatccgg cgcaccagat tttaacgttg ccaccgcagc acccagtttt
     3481 tccaccagcg tatcgtaaat gcctttttgc gcgtagatcc gacaagccgc agtacaatcc
     3541 tgtccagcat tgtaatagcc aaatgtacgt acaccttcga ccactgcttc aatatccgca
     3601 tcatcaaaaa caatcactgg cgctttgcca ccaagttcca tatgagtacg cttaatggac
     3661 gacgcggtat ggctgatgat gtgctcgccg gtggcgatag agcccgtcag cgacaccatc
     3721 cgcactttgg gatgaccggt cagcggatca cccaccgttt tgcctctgcc aaacagtatg
     3781 ttaatcacgc ctgccgggaa gatatctttc gccagctctg ccaacttcaa cgcggtcagc
     3841 ggggtaattt ctgatggttt aagcactacg cagttccctg ccgccagcgc cggagcaagt
     3901 ttccacgcgg ccatcatcag cggataattc cacggtgcga tagaagccac gacccccaac
     3961 ggatcgcgac ggatcatcga agtatgacct tcaagatatt cacctgccgc cagaccattc
     4021 agacagcgcg ccgcacccgc gaaaaagcga aaaacatcga caatcgccgg gatttcatca
     4081 ttgaacgcac tatgcagcgg tttgccacaa ttacgggatt ccagttcggc aaaaacctga
     4141 ccattttctt cgataacatc cgccagtttc agcaggcatt ccgcacgcgc tttcggtgtg
     4201 gtttgccccc attcagcaaa tgctgcatct gccgcacgca cagcagcatc gacctgctct
     4261 gcggatacct cggcaatctc cagtaaaacg tcccccgttg ccggattata gacaggctgt
     4321 ttttcccctt cgccgctaac ccgttctccg ttaatcagta acttatgttg catagcttat
     4381 tcctgtatca gtggttattg accattgtcg ccttcgcggg ttagccacca ggcccccagg
     4441 atcggcaagg ttgttaccaa cataaccagc agtgccacca cgttggttac cggtacatca
     4501 cgcggtcgcc caagctgatt gagcaaccac aacggtaacg ttcgttcatg acctgccgta
     4561 aaggtcgtaa cgatgatttc atcgaacgac aaggcaaacg ccagcattcc tcctgccagt
     4621 aacgccgaac tgagattcgg caacactacg tagcggaagg tttgccagcc attggcccca
     4681 agatccattg acgcctcaac cagactccac gaggtgcggc gaaaacgggc gatgacattg
     4741 ttaaacacca ccactacgca aaaagtcgca tgaccgacca cgatggtgaa aaatcccggc
     4801 tccagattga tggttttaaa ggcggttaat agcgccagac cagtgacaat gcccggcagc
     4861 gcaatgggca gcagcaataa cagcgaaatg gcgtttttgc cgaaaaagtc tcgtctccac
     4921 agcgcggcag ctgccagcgt ccctaacacc agcgcaatta atgtcgccag cgccgccact
     4981 ttaagtgaca gcgtcacggc atcaagaata tcactacgct gtgctgccac gctaaaccag
     5041 cgcagcgtca ggccctgcgg tggaaaacta aacgccgcat cttcagtgtt aaacgcatag
     5101 gcggcgatta tcaggatggg aaaatgtagg aaaacaacgc cgccccaggc cgccagtttg
     5161 aggaaaaacg gtgcgcgttc agagtgcatc gaacgctccc agacgtttca cgaacgccag
     5221 gtacagtgcg atgagaataa tcggcaccag ggtgaatgcc gccgccatcg gcatattgcc
     5281 aatcgccccc tgctgggaat aaaccatatt gccgataaaa tatcctggag gaccaaccag
     5341 ctgcgggacg ataaaatcgc ccagtgtgag tgagaaggta aagatagagc cagcggcaat
     5401 acccgggatt gccagcggca gcaccacata gcgaaaggtt tgtcgtggac gtgcgccgag
     5461 atcagccgac gcctgcaaca atgacggcgg caaacgctca agcgccgcct gaacgggca
//
LOCUS       DAJTYH010000177         5437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTYH010000177 DAJTYH010000000
VERSION     DAJTYH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(85..246)
                     /locus_tag="OO609_003827"
     CDS             complement(85..246)
                     /locus_tag="OO609_003827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635061.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(243..431)
                     /locus_tag="OO609_003828"
     CDS             complement(243..431)
                     /locus_tag="OO609_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6635062.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            691..1026
                     /gene="iraM"
                     /locus_tag="OO609_003829"
     CDS             691..1026
                     /gene="iraM"
                     /locus_tag="OO609_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6635063.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1307..1415)
                     /locus_tag="OO609_003830"
                     /pseudo
     CDS             complement(1307..1415)
                     /locus_tag="OO609_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1938..2014)
                     /locus_tag="OO609_003831"
     tRNA            complement(1938..2014)
                     /locus_tag="OO609_003831"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1978..1980),aa:Arg,seq:tct)
     gene            complement(2116..2191)
                     /locus_tag="OO609_003832"
     tRNA            complement(2116..2191)
                     /locus_tag="OO609_003832"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2156..2158),aa:Ile,seq:cat)
     gene            complement(2336..3157)
                     /locus_tag="OO609_003833"
     CDS             complement(2336..3157)
                     /locus_tag="OO609_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6635064.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3172..3528)
                     /locus_tag="OO609_003834"
     CDS             complement(3172..3528)
                     /locus_tag="OO609_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6635065.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3541..4590)
                     /locus_tag="OO609_003835"
     CDS             complement(3541..4590)
                     /locus_tag="OO609_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6635066.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4592..4870)
                     /locus_tag="OO609_003836"
                     /pseudo
     CDS             complement(4592..4870)
                     /locus_tag="OO609_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4937..5188)
                     /locus_tag="OO609_003837"
     CDS             complement(4937..5188)
                     /locus_tag="OO609_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635067.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
BASE COUNT         1461 a         1449 c         1143 g         1384 t
ORIGIN      
        1 ctgataactc atgcaagcgt ctcagacgac ccactgacac taccggtgag tttaacgata
       61 ccttccattt gactggctca ctttttatga tgatgccggt gcatttatct ccagcaccag
      121 actttctatc tcaacgccat acgttgcatt tttggtaata tccgtcagcg tcagtgcatt
      181 tagtcccact gccagactgt cttttatggc ctggaatgcc gggccagtac gatgacgtag
      241 tatcactccg gctcagttgc accactgacc accacatcac cttctgctgc aatcgcctgc
      301 atcagggtat aaggggttat ggccaccgga ctaccaaacg gctgccagcc ctctttcagt
      361 ttatgtgtca gcttttccgc aagatctgac ggcggcgccg ccctgacaac atcatagtgt
      421 ttaaatgcca tggttctttc caacatctga aaaataattc tttaaaatac ctgacatgta
      481 atacagaaaa aacacaaaac cataccttaa ataaaaacct gattatcaag cagatatgca
      541 tggataaact acaagacgag atataaacca ccctgcattt aaataaacaa taaacaacat
      601 cagaaaaata attctgttct atggtttaat tcaaaaatat catttatact tttcagaaca
      661 tcaccagcaa ggcataaaca aggaaactaa atgaagtgga ttgtgattga tacagttatc
      721 cagccatcat gcggaatatc tttttcagtc atatggagta aaataaaatt aataatctgg
      781 tatcaatcgg atgctttctt acctcctgaa agtatattta cactgactca cacaggcatc
      841 atgctcaata acaaagtgct acctgtaacc atttacaacg tagtaccatt caataaaaca
      901 ttctggaatt taatcaaaaa cagccaggaa tgccctacaa atacagataa cgtattgaat
      961 gaatgcttta ataaccgttg cactctgcaa atatgccctt atggactaaa acaacaaagt
     1021 ccataaggag tttactcaca tctgacaaaa tcaatataaa cagcccctcc ggagaggggc
     1081 tggagagtgg cgctatgtgc cattccatgg tgccgggtgc ctcccggtga attcagtacc
     1141 agcacctgaa tccgcgatta tcccatatac ctactcgctg attgcccctc cgcacagggg
     1201 gattcaccat gccagtttct tttaacaaac tccccgcaaa ccagacaaca gtcaaccgcc
     1261 tgaattgtga agtatttaaa aatttctccc gctaactgat acccggctaa cagtctggcg
     1321 ttttcttttt cagcaacggg aaagcaacaa ccaccacacc cgccaccagc acaccgtcag
     1381 ccggcactga cattatccgg ctgctgcaat gccattaaca aaaacagtaa gcaatcactt
     1441 tttaccgtaa caggtgataa tccagatatg tatctacccc agatgagtaa tccgaagttc
     1501 atccatacca caggtcctgg ctattctgtt gtactcctga acaagagcaa ataattctga
     1561 attagcaacc atgaactcat cgcaaaccct ctgtatagca tcactattca gaataataac
     1621 gtctcttccc gaaagacgat caggagtaca gaacaaaact gtcaaacggc tgaaggcctt
     1681 tgctcgtgct gcattgacta tatcaatacg ctgcctaagg atgaaacacc ccgacgcctc
     1741 atcaatattc actctaccca caccatatga atgataaata cttaatgctg aaaaaaccat
     1801 tagaccgtat aacaaatact caatcaatac ttaacagaac ttttattttt gacaaatata
     1861 taatattttc aacaatatcc tgagccaggt atatttcagt ataaggctct gccggaagga
     1921 atctggaaga atgaatatgg cgcgctgtac tggattcgaa ccagtgaccg attgcttaga
     1981 aggcaattgc tctgtccggc tgagctaaca acgctgaata ccgataatgg accgccatcg
     2041 gggacccgcc ccccgcacca acaaccctgt tatcgtgtcg tctgctcttc ctgataagct
     2101 aatggcggtt tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag
     2161 tcgctcgctc tcaccactga gctaaagggc cgggagcaga ataataacgg tccgtaatta
     2221 atttcgcaat aaaaaaaccc gctcagcggc gggttgtaga aactcttcta acgccaggca
     2281 taaaacgccc atcattatga cgaatttacc acagattccg gaaaaatcaa ccttgttacc
     2341 tagttacctt ttttaactgc cgctcagccc atgcttcttc aatatcaaac cgggtcacca
     2401 gcgcatcata aaatttctta actgtttttt cccatgacgc gcgtgttatc tggtttgtca
     2461 cctcgcatat agcattaaat gcctccgttg atggtagtct ttcatagcca cgaccaccac
     2521 aacgctggca gtctctgata acaggcatac cacgttttac cgactcttca cgatgaatgg
     2581 cgacaccacg cccacggcaa tccttacagg cggtggaaac ctcacccttt ccgccacact
     2641 ccggacaggc aacttttacc acctccctga cttttttcca ttcttcccag taaaacggat
     2701 acacaccttt cgtacacttt gcccataccg gcggcttacc atccggatac tggatcttgt
     2761 ttgtaaaaac tatgctttca ataaattttt ccccatagca acaagggcac tgctttttac
     2821 tcgctgcgct gcgggcataa tcctcaaaag cgtacgaagc cataatgcgc atcactaccg
     2881 gttttatttc tgccggaagt tttctcaacg ccgccacacg atcgcaccga ctgagtgcat
     2941 aatctgccag taattctgtt gcccgcgccc tgtcattcat actgatgccc attttcccca
     3001 ggaacgcaga aaaacccatc tcagcccgat tctgtgtcat gccctgcgcg gccatcacat
     3061 cagtgatact cagcgcatct tttgacgttg aggccgatgc atcggtcagg ccaggggatt
     3121 ttggggagta gtatttcggt aaatcttcca gtttcatttt ttgacctgct cttaatgcat
     3181 tatggggtaa atcttcaccc ccagacgtcc accagatact ggctgaccac gaacgatatt
     3241 gatttcatca aactgctcat cgtccattaa cactcccgca tgcgtcagcg catccagcgg
     3301 tgctttcagg atattgtcca ggtcgcgacg acgcttatcc ggtggctctg caatcacctt
     3361 tatcgccagc cttccggaca ggcttaattt cagccgctgc tggcgaacaa taagcgccac
     3421 agcccggcga taacgctttc cctcctccga gataaaatat gtgctgccac ggcgtcgcca
     3481 gtaagtgttc accgtcggcg ggtaaggtaa aaccaaatct atgagcatca gtcacctctt
     3541 ttacccaagc acgccagttg caaaggcgtg atcaagaaaa cgaaaaatta aatcaacctg
     3601 agaaccatgc ttttcttcga acgccagagg atccgcatga agctcgttgt gatgctcccg
     3661 acacagcggt agcgtgaaaa tatcgtgaga tttggtcccc attccgccct gaccatgacc
     3721 aatcaggtga tggggatcgt cggctggctt accacaacac gcacacggct gtgtcttcac
     3781 ccagcgtttg tatttctcgt taacccagcg gcgacgttta ggtcgtttca tgaaagattc
     3841 cggagactca ggatcaacgg caatgctgac caccgtcttt tcctgttgtg ggttctgttg
     3901 ctggtgggcg tgaggcagcg gcgcaagatt ttttgtgcgc tgcttcagta tgctggtggc
     3961 ggtctgctct cccggcacga tgtcgctttc acggtacatt gagcggattt tttccgcacg
     4021 caaccccagc gaacgacgta ataccgcttc cggtagcgcg tccgccacct gattgcggac
     4081 cgcccaccag gataattcag ccagagataa ttcacgctcc tgcgtaccgc ttattgcgtg
     4141 accgatgacg tcaatcatcc atgctgacag gttttgatga gcaagttgct cgagtgattc
     4201 ggatgtctgg tcacgcagct ggttgtcgca gtgccagcac aataccattg cgccggtacc
     4261 ataacggtga atgacggttt cgctgtgatg ataatcgccg tgtggccact ggcaggattt
     4321 aatatggcgc aacagccagt cagacaatgc gccagcacca ccagcagcac gaatcacccg
     4381 tgcgttactg aaaaacggca gcaatgtttt gtcttccact agcggctggc gaacggcagg
     4441 aacgaccccg gacggcagat tacgcatgct tttcggttcc ggctccacca gtacccgggt
     4501 attgtggaat accggcatgg attcacgacc gggcttaagg accaccagcc cgagttccgg
     4561 aaccagaaca ggtcgaagta atacccgcac gttacctcca gatccgttgc tggaatgtgc
     4621 gggacggacg cggtggctgt tcggagtaag ggagcctgac ggagattatc tagtgacggt
     4681 agtcgaggct aagggctttt ttaacctcgc atccgcgcct gcggtaacac tgaataagcc
     4741 attcggcctg ttcttcagtg catgagggat gctggtacca gtctgactta aatgcgtgag
     4801 aataccgctc gtgcgtgtgg gcaagaacgg tcgaattatc atgattgtaa tattttgcgt
     4861 tgcgtgccat cggttttctc cggtggcacg gtgttactca gcgggagttc agccccgcgc
     4921 aagattgtag atgagtttat tcttctgaaa aagcagaaaa gccagctttt attccgatct
     4981 ctttcaatgc ctgtaatgaa gtgacaaact caccgtcgcg caagataaat ccgtccgtca
     5041 ctcgggcatc cacaaaatta attaacgcag ccccattttt ttgcaaacac acaatgcggt
     5101 aatgactaac aatatttcca ttttcaacgc acacagcata gaggccatct tcacaaaaaa
     5161 ttttacgcag ttcttcgatg ttcatcatca gaatccttcc agataagtag cactcccctg
     5221 ttcggggtcc atccctttta tccctgcgcg ctacttaagt atttttgatt ctattccggc
     5281 accatccaga acttcaaatg cgttgaaaat aaaaacaaaa aaccgccgaa gcgggttaag
     5341 tgcgggtgcg ttgaggatgc ctgccacatc agaggtggcg agggatttct cactcgccgg
     5401 gtctcttact cctcaggttc gtaagctgtg aagacag
//
LOCUS       DAJTYH010000178         5430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTYH010000178 DAJTYH010000000
VERSION     DAJTYH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            202..386
                     /locus_tag="OO609_003838"
                     /pseudo
     CDS             202..386
                     /locus_tag="OO609_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            529..750
                     /locus_tag="OO609_003839"
                     /pseudo
     CDS             529..750
                     /locus_tag="OO609_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(771..833)
                     /locus_tag="OO609_003840"
                     /pseudo
     CDS             complement(771..833)
                     /locus_tag="OO609_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1283..2695
                     /gene="ospC1"
                     /locus_tag="OO609_003841"
     CDS             1283..2695
                     /gene="ospC1"
                     /locus_tag="OO609_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6635068.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2799..2948)
                     /locus_tag="OO609_003842"
                     /pseudo
     CDS             complement(2799..2948)
                     /locus_tag="OO609_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3024..4721
                     /gene="ospD3"
                     /locus_tag="OO609_003843"
     CDS             3024..4721
                     /gene="ospD3"
                     /locus_tag="OO609_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6635069.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4838..4957
                     /locus_tag="OO609_003844"
                     /pseudo
     CDS             4838..4957
                     /locus_tag="OO609_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
     gene            5124..5396
                     /locus_tag="OO609_003845"
     CDS             5124..5396
                     /locus_tag="OO609_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635070.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT         1820 a         1036 c         1053 g         1521 t
ORIGIN      
        1 tgcgtcttct tcgtcaaaaa aatcatcttc gtgaagcttc acgacatagc ggggaagttt
       61 gcttctttga gaggcgggtt tacgtttacg ggatttagct gaacgggcca tataaccacc
      121 tgaaagacaa tgacattgcc tgtttttata acggtaattg cagaccatga caaaccgcag
      181 ccgtcaggct gcctactcga gtcacaaggc tctttgtgtc cgatatgccg gcagcgcatt
      241 acccatgaaa ctggctggaa cgtacaccat aaggtgaaga aagttatggg ggcggtgaag
      301 aattatctaa ccttgtcttg ttacatccaa attgccatcg acagttacac agttgtgaag
      361 ccggctctct caaaaagggc ttataaaggc ttgagcggtg taccgggaaa ccggtatgca
      421 ccgttcttag gggagggcag tccagcaatg aactgtcctt acccgacaaa catccaaaac
      481 gaacggaacg tgctggaatc cgcatataac ccgttgtaaa gtgaggaaat ggacacctca
      541 cttgctcatg agaacgcccg cctgcgggca ctgttgcaga cgcaacagga cactatccac
      601 cagatggctg aatacaaccg cctgctctca cagcggatgg cggcttatgc ttccgaaatc
      661 aaccgactga aggcgctggt tgcgaaactg cagcgtatgc agttcggtaa aagctcagaa
      721 aaacttcgcg caaaaaccga acggcagatt ccattttcac gggcgatcta cgccacacag
      781 gctctgggtt gcagtgactg ctccattaag gcgattttga actcataagg gaagtaacaa
      841 aaaaactgca aaagtacaat aattatatat ggccaaacta acctctatta aaaaacatca
      901 gtttttcaca cttagttgag cttcatgaaa aaatcatcga ggatttcatt gcagaataca
      961 cattccacta actggaagca ctccataaga ggaatcagag aaagtatcgt ttttttatag
     1021 taaaattcat tgctgttcaa taaaattgat atagttatta aattatgaaa ataatttcac
     1081 aaaaaagatt aacttgagtt cttaagcatc ggattttaga tattttgaaa tatctggcat
     1141 cacataacat caaaagtata taatactctt atctaattac atgcagtata ttgtatatat
     1201 actcaaaaat tgtaattcat accctgctat atcttgtagg gaaagatatt aattaaaact
     1261 gttttcatat aaggttcatt ttatgaatat atcagaaaca ctgaactcag caaataccca
     1321 atgcaatata gattctatgg ataacagatt acatacattg tttccaaaag tgacatcagt
     1381 gcgaaacgct gcacaacaaa ctatgccaga tgaaaaaaat ttaaaagata gtgcaaatat
     1441 tattaaaagt ttctttagga aaactatagc agcacagagt tatagtagaa tgttctctca
     1501 aggctctaac tttaaatctt taaatatagc aattgatgca ccatcagacg ctaaagcctc
     1561 atttaaggct attgagcacc ttgacagatt atcgaagcat tatatatctg aaataaggga
     1621 aaaacttcat cctctttctg cagaggaact caatttgctt tcgctaatta ttaattctga
     1681 tttaatcttc agacatcaaa gtaattctga tttgtctgat aaaattttaa acattaagtc
     1741 attcaataaa attcagtctg aaggaatatg cacaaaacga aacacatacg ctgatgatat
     1801 aaaaaaaata gctaatcatg actttgtgtt ttttggcgtt gaaatctcta accatcagaa
     1861 aaaacacccc ctgaatacaa aacatcacac tgttgatttt ggtgcaaatg cgtatatcat
     1921 tgatcatgac tctccatatg gatatatgac attaaccgat cactttgata atgctattcc
     1981 acctgttttt taccatgagc accaatcatt tttagataaa ttttcagagg ttaataaaga
     2041 agttagtcga tacgtacatg gaagtaaagg aattatagat gtaccaatat tcaatactaa
     2101 agatatgaag ttagggctcg gattatacct gattgacttt attagaaaaa gtgaagacca
     2161 aagcttcaag gagttttgct atggaaaaaa tcttgcccct gtggatctgg atagaatcat
     2221 aaactttgtt tttcagccag agtaccatat acctaggatg gtaagtacag aaaacttcaa
     2281 aaaagttaag attagagaaa tatccttaga ggaggctgtt acagcatcta attacgaaga
     2341 aattaacaag caggtcacta acaaaaaaat tgctctccag gctctttttc tttcgattac
     2401 taatcaaaaa gaggatgtcg ccttatatat attatctaat tttgagataa ctagacaaga
     2461 tgttatttcc ataaagcatg agttgtatga tattgagtat ctacttagcg ctcataattc
     2521 aagctgtaaa gtacttgagt attttatcaa taagggattg gttgatgtaa acacaaagtt
     2581 caaaaaaact aatagtgggg attgtatgtt ggataacgca ataaaatatg agaatgcaga
     2641 aatgataaaa ctattattga aatatggtgc aacatctgac aataaatata tttaatcaaa
     2701 attgaatatc gtttagcaaa aggcagtatt tgcacaaaat atcgatatat tgtttattgt
     2761 cagtatggct caatgtgata atagttggaa agtttgatgg gtttcgcccc gttgtagcgg
     2821 tagtcgaact ggaaggtctt caggcactgc ttacagcgat agagcagccc ccctgaactg
     2881 gaatggccgt tccggatacc cccctgagtt tcagagtaac ggtgacaaac cacatcaatc
     2941 tttgccatca atcatccaaa gggcaaagag tacaacaaca ctaagtctgc gtcacaaccc
     3001 atcaatgaaa ggaatatata catatgccat cagtaaattt aatcccatca aggaaaatat
     3061 gtttgcaaaa tatgataaat aaagacaacg tctctgttga gacaatccag tctctattgc
     3121 actcaaaaca attgccatat ttttctgaca agaggagttt tttattaaat ctaaattgcc
     3181 aagttaccga tcactctgga agacttattg tctgtcgaca tttagcttcc tactggatag
     3241 cacagtttaa caaaagtagt ggtcacgtgg attatcatca ctttgctttt ccggatgaaa
     3301 ttaaaaatta tgtttcagtg agtgaagaag aaaaggctat taatgtgcct gctattattt
     3361 attttgttga aaacggttca tggggagata ttatttttta tattttcaat gaaatgattt
     3421 ttcattccga aaaaagcaga gcactagaaa taagtacatc aaatcacaat atggcattag
     3481 gcttgaagat taaagaaact aaaaatgggg gggattttgt cattcagctt tatgatccca
     3541 accatacagc aactcattta cgagcagagt ttaacaaatt taacttagct aaaataaaaa
     3601 aactgactgt agataatttt cttgatgaaa aacatcagaa atgttatggt cttatatccg
     3661 acggtatgtc tatatttgtg gacagacata ctccaacaag catgtcctcc ataatcagat
     3721 ggcctgataa tttacttcac cccaaagtta tttatcacgc gatgcgtatg ggattgactg
     3781 agctaatcca aaaagtaaca agagtcgtac aactatctga cctttcagac aatacgttag
     3841 aattactttt ggcagccaaa aatgacgatg gtttgtcagg attgctttta gctttacaaa
     3901 atgggcattc agatacaatc ttagcatacg gagaactctt ggaaacttct ggacttaacc
     3961 ttgataaaac ggtagaacta ctaactgcgg aaggaatggg aggacgaata tcgggtttat
     4021 cccaagcact tcaaaatggg catgcagaaa ctatcaaaac atacggaagg cttctcaaga
     4081 agagagcaat aaatatcgaa tacaataagc tgaaaaattt gctgaccgct tattattatg
     4141 atgaagtaca cagacagata cccggactaa tgtttgctct tcaaaatgga catgcagatg
     4201 ctatacgcgc atacggtgag ctcattctta gcccccctct cctcaactca gaggatattg
     4261 taaatttgct ggcctcaagg agatatgaca atgttcccgg acttctgtta gcattgaata
     4321 atggacaggc tgatgcaatc ttagcttatg gtgatatctt gaatgaggca aaacttaact
     4381 tggataaaaa agcagagctg ttagaagcga aagattctaa tggtttatct ggattgtttg
     4441 tagccttgca taatggatgt gtagaaacaa ttattgctta tgggaaaata cttcacactg
     4501 cagaccttac tccacatcag gcatcaaaat tactggcagc agaaggccca aatggggtat
     4561 ctggattaat tatagctttt caaaatagga attttgaggc aataaaaact tatatggaaa
     4621 taataaaaaa tgaaaatatt acacctgaag aaatagcaga acacttggac aaaaaaaatg
     4681 gaagtgattt tctagaaatt atgaagaata taaaaagctg aatattatct cgtggtgata
     4741 cactgaattt gaggtaaccg gctcatttaa accgtctggt ctgttacctc cggttccaca
     4801 aaaataatgt ccatcatttt gaagtggaca ctatcgtatg gaataccgga cctggatcac
     4861 tgaagcttta cgccttcatt tcgaagaaca tttaccacgg gttgtggccg gacgtcgcct
     4921 gggcgtacca aaatcaactg tctgtggtat gttcgtgtga accgcccccg gtatcccgct
     4981 catgctggcg aacccagaca cgcagtgaac cgccccgggt ttcctggaga gtgttttatc
     5041 tgtgaaccac ggcagcctgg cggctgcaaa tcggggtgtg gcagaatatg agctttcaga
     5101 gtaaccatcc agaatgagtt cccatgtcac acaacctgga gcaccagaaa gtccacacca
     5161 gaatggtgaa agaggttctg aaagccgtcg ccagagccaa taatcaccct tataagtccg
     5221 tatttgcaga ctttatcacc ggtcatccct cctgcacggt ctgtttctgg gaaaccttcc
     5281 acaaaatgta cccggattca ccgtacgaat acgtcacgtt ctgccatacc tgccgtcgtt
     5341 ttgatttata cgaaacagaa gcggagatga aggcggatga cccgaagtgg tggtgatttt
     5401 cagccccgac cgctgaaacg cctgttcaca
//
LOCUS       DAJTYH010000179         5370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTYH010000179 DAJTYH010000000
VERSION     DAJTYH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(21..428)
                     /gene="nudG"
                     /locus_tag="OO609_003846"
     CDS             complement(21..428)
                     /gene="nudG"
                     /locus_tag="OO609_003846"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6635071.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            515..1135
                     /locus_tag="OO609_003847"
     CDS             515..1135
                     /locus_tag="OO609_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6635072.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1144..2451)
                     /gene="ynjE"
                     /locus_tag="OO609_003848"
                     /pseudo
     CDS             complement(1144..2451)
                     /gene="ynjE"
                     /locus_tag="OO609_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(2518..3171)
                     /locus_tag="OO609_003849"
     CDS             complement(2518..3171)
                     /locus_tag="OO609_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6635073.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3171..4642)
                     /locus_tag="OO609_003850"
                     /pseudo
     CDS             complement(3171..4642)
                     /locus_tag="OO609_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(4615..5370)
                     /locus_tag="OO609_003851"
                     /pseudo
     CDS             complement(4615..5370)
                     /locus_tag="OO609_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1237 a         1474 c         1434 g         1225 t
ORIGIN      
        1 tgatgacaaa acccttagca ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc
       61 tctaataatg gaatgtcagc aggggccagc ggatattgca gcgcctcttc aggtgagcac
      121 cagaccagcg cctgatgttc atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag
      181 gcatgaagat ggataatccg gcccgaaact tctcgctgat ggctggcaac atactcaccc
      241 acagttgctt cgatgtccaa ttcttcgttt aactcacgca ccagcgcctg ctgctggctt
      301 tcatccagct cgactttacc tccggcaaac tcccataatc ccgcctgatc gctctgggcg
      361 gggcgttgcg cgagtaaaat tttgccatca cgttcaatga tggcggcaac aacttcaatc
      421 attttcataa attacacacg ggcagataag cgaaaaatgc cataatatcc gcactgttgc
      481 tcaatcagaa aggctttata tttaaggagt gcctgtgcta gaccgccatc ttcatccccg
      541 gattaaaccg ttgttgcatc agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga
      601 tggtttaaca ttagtgggat ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg
      661 ctggtatctg gcggcgctag tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg
      721 tgcgctggcg cggcgcagag ggcttaccga cgcgggcggc tttcttgata tttctctcga
      781 ttttctcttt tacgcgctgg tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc
      841 gctggcaggc ggctggttgt tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt
      901 tgctgcactg gcagcaaaac atcagattga taaccccggt tatgcacata agtcgtttta
      961 ctatctgggg ggattaaccg aaggcacaga aacgatctta ctgtttgtgc tgggatgttt
     1021 attccctgca tggtttgcgt ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac
     1081 ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc agtaattgca
     1141 atgttatttg ctactgcccg ggccgcgttc accggttgca accggatttt ttggatcgct
     1201 gctccattcg taccagccgc cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc
     1261 gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc
     1321 tggtttgata ttccatgctt tccacatagc ggtaatatca tcggcgctac gcatggtgcc
     1381 atccgggtta tagaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc
     1441 tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac cgataaattc
     1501 tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc cacgggcctg
     1561 ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat ccggttccgc
     1621 tttcactttc ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca
     1681 gccgccatcc agcagacgca catctttcac gcctgcgtag agcataattt gcgccacacg
     1741 cgctccagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg
     1801 ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg gttcgctttc
     1861 cacttcgttg gtatcgatgt agtcagcgcc aggaatatgg ctgataaggt aaagcttagg
     1921 agcgccctag gccgcttcaa tgactttcca gtcaccgaca ggtttcgccg taacctcttt
     1981 accttgttgc aggtcatgca gccattgcgg ataaaccagc tgctcaaagt gcggcagttt
     2041 ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg taaaacctgc
     2101 tttttgcagt cgcgttttga cggcgtcaac atctttgcca ttaccgtaca gcgccaccgg
     2161 ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg tgctcatttt
     2221 gtccagccag ctagcagaga ggtttaaggc ggcaggttca tgaccagaag ggccatttaa
     2281 ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tctatcgctt tgccattttg
     2341 ttgctgaagt tggtcaagtg taagaggctt cgccagttca gcggcccacg aagaagcgca
     2401 agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca tcgaaaatcc
     2461 cgacattgta gttttgagtg cgccattctg aactgacgca ttgagaaaaa ctttgcgtta
     2521 tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac tatcagcagg
     2581 aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagggcgc gaacttcgct
     2641 gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga atggctcatc
     2701 caggagtaac gcttttggct gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg
     2761 accgccagac aaagtggcag gatcctgatg gaaagctccg tcaaggcctg aacgctcaag
     2821 tgcatcattc acggcattac gtcgggcatt ccccttaagt gtcgccggta gcgccagcag
     2881 taaattttgc ccgacgctga actggtcaaa taacagtgca tcctgaaaaa gaatgccaat
     2941 ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata gctcacctgt
     3001 acaagaaaac tgtccggcca gtgcaccaat catccatgaa aacagagtgg attttccaca
     3061 gccagacggc cccattaacg tgacgatgtc acctttatca accgtaaagt taacgtttgt
     3121 cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca ttagcggatt
     3181 ccttgtctga cataacctac ccattttgcg actaacgcgg tcagggcaaa aataataagc
     3241 ggtaacaaca gttgccataa cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt
     3301 aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc
     3361 atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcac agggcgcaac
     3421 atgagtgggc atttcacgta aaagaagatt ttggcccgcg accagcccag tgtttgcgca
     3481 atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa cagcatccac
     3541 ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc caggttcagc
     3601 catagcgcca gcgtgtactg gcccgccacc agtggcagag cgggcaatag aatgggcaac
     3661 catagccaca actggcgacg ctgtgccccc cattccagcc acagcagtaa caggagcaaa
     3721 gcgatgaatg ttgctaccag ccccattgtc agactgttga tgagtgcttc actattgatc
     3781 gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt taagggtaaa
     3841 aaactcgcca gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat gccatctacg
     3901 cggggaatag tgcgccgcca gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag
     3961 cagactggcg agtgcgcctt tcgtttgttg atccgcgtcg ccctgagtta accactgcca
     4021 gctaattact gccagcgtcg gtggattacc tggcccaaga ataatcgcca catccacgac
     4081 cgaaagcgac caggcaacaa tcgccagcat cgccattgcc agcggctgta gcccagcgaa
     4141 tccagcacaa tgacctgctg caacagccgt ttttcgctca atactgccac taagatccac
     4201 agcagaaatg cgctttcttt cactgcaagg gtgaggccca gaccgatcca aatcggtcca
     4261 ttggcggagt gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca
     4321 gggcgctggt ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca
     4381 tacgctgcca tttcggccca ggccatagcg ccacaatcac cagcagggca atcaacaatg
     4441 ccccgaccgc cgcgatggtt gtcgacacca gcgttgccag taatgcctgc ggtaactgtg
     4501 gatcggcaaa taacgcctgc cagcgtgtca acgacaacgc aggcgatatc agcgtgagag
     4561 ctgccgggat cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg
     4621 cgtaccgtaa cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc
     4681 agccagtacc ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc
     4741 aggcagtttt tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg
     4801 cagttgcgca tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt
     4861 cgcgttggct ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa
     4921 actgtaactg cttgccggca aatcgccgct ggcgattttt tgctgcgcat gtgcggggtt
     4981 aaaggtcagc gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga
     5041 aggcgggaaa tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcta
     5101 caagggcgca gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg
     5161 atctggcgtc agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg
     5221 gcgcggataa gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt
     5281 ttgtggtggc tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca
     5341 gggcgattcc gcaccttgtg tcggcactga
//
LOCUS       DAJTYH010000180         5363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTYH010000180 DAJTYH010000000
VERSION     DAJTYH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..398)
                     /locus_tag="OO609_003852"
     CDS             complement(<1..398)
                     /locus_tag="OO609_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6635074.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPG"
     gene            complement(395..1270)
                     /gene="nanK"
                     /locus_tag="OO609_003853"
     CDS             complement(395..1270)
                     /gene="nanK"
                     /locus_tag="OO609_003853"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6635075.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1267..1956)
                     /locus_tag="OO609_003854"
     CDS             complement(1267..1956)
                     /locus_tag="OO609_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6635076.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2004..3494)
                     /gene="nanT"
                     /locus_tag="OO609_003855"
     CDS             complement(2004..3494)
                     /gene="nanT"
                     /locus_tag="OO609_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6635077.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3604..4497)
                     /gene="nanA"
                     /locus_tag="OO609_003856"
     CDS             complement(3604..4497)
                     /gene="nanA"
                     /locus_tag="OO609_003856"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6635078.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(4619..>5363)
                     /gene="nanR"
                     /locus_tag="OO609_003857"
     CDS             complement(4619..>5363)
                     /gene="nanR"
                     /locus_tag="OO609_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6635079.1"
                     /translation="AIGRNLRSRPLARKKLSEMVEEELEQMIRRREFGEGEQLPSERE
                     LMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADTIIGELSGMAKDFLSHP
                     GGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQSLDNNAAFIRSDVDFH
                     RVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVSYQQHIAIVDAIRRHDP
                     DEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1285 a         1516 c         1391 g         1171 t
ORIGIN      
        1 catcccggct tatgcggctc tcctggcata aacacggcga acattcccgg tttcagaatg
       61 atggtttgct cgttttcaat ggcgctacaa agctggtaat catcttcatg gtggaactct
      121 tcacattggc gcgcagtgcc tgccatgcca aacagaatcc gttcctcacc gtttaataac
      181 agctggatat caatgtattg ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg
      241 ttaaacgtca tgacattcat aaaaatattg tcgccctgta attcgtaacg acccggcgct
      301 ttttcttgcg gtctggcagc taaggccagc gttaacgcgt cctgtaacgc aggatgtaac
      361 ccagcagacg gtaatgactg tacttcaccc atcatcataa tttttctccc tgggccaaca
      421 gcgcagcccc aagtaaacct gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa
      481 atgccgctgg ctcctgcgcc agatacattt ccaccaacgc cagataccct tctgccagac
      541 caacgctgcc accaaccacc acgcactggc aatcagttgt ggctttaata tcagcgatca
      601 gccttgcaag cgtacgtgcg gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc
      661 cggcgcgtgt gaaaatagtt ctcgcatccg cgccagccag ctcgccctgc gctgccgccg
      721 caatgccgcg accagaagca attgcttcca cgcaacctgt gcgtccacag ccgcagactg
      781 ggccgtgtgg atcggcaagc gtatgcccga tgtgccccgc cagaccgcca gggccggtaa
      841 gcagtttgcc gccgctcact acaccgccgc cgacgccagt ggaaacggtg ataaagacca
      901 tatcggttat atcgccttcc agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt
      961 taatggcaat ggtcggcaaa ttggtaagtt gttccagcgt tttgactaac ggaaagtgta
     1021 gcaatccacc gagattatgc ggattcagcg ccagcaaact gccgtcgcga ataattccgg
     1081 ttgaagcgat ggcaacccgc tgcgcatgag cttgcaacgg agagactaat gcggataagg
     1141 catcacgcaa ggcttctggt gtctggctgg ctggcgtagg aagttcacga cgatcgcgga
     1201 tctgcccgtc agcgccaatc agcgcggcgg caagtttagt accgccgata tcaatcgcca
     1261 gtatggtcat agcaccgcct ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag
     1321 acgcgtaatg gcagaaccga ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc
     1381 ctgagcaggc gtgttgtaac gcccttcggc aatcacccga catccggctt cgctcaacgt
     1441 tttcaccagc gccagatccg gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt
     1501 agtgccaata atttcggctc ccagcttttg gcatgccagg ccgtcttccg gcgttgagca
     1561 gtcggtcatc gccagtaaac cgtgatgatg gatacgtgcc agcagcgttt caacaggcac
     1621 cggacgcggg cggtcggtgc cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc
     1681 atcgacatct tcaatatagg ccgtgatgcg taccggagaa tcctccagat cgcgtttcac
     1741 aattccaata atcggcacgc tcaccaccgc acgcgtggct tgcagatttg ccacaccttc
     1801 gatgcgaatg gcaaccgcgc ccgcctgctc tgccgctaat gccatggctg cgacgatttc
     1861 gggtttatcg agcgggctgt caggaaccgg ctggcaggag acaatcaggc caccgttagc
     1921 agcgattttt tgatccagtt gtgcaagtaa cgacatacat cttcccttag cgaaaggccc
     1981 ggtacataga ccgggcaaca ggattaactt ttggttttga ctaaatcgtt tttggcgctg
     2041 ccaaatggca cggcaccgct aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct
     2101 tccgggcgca gccaacgctg aacgcgggaa ggcatatcca gaccaatcag caggatcacc
     2161 acaaatgtca ggctgaacga gagcgatgcc agcgcagtac ccagatccag acgttgagcg
     2221 atcaacgcgc cgatgattgg ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag
     2281 cccaggcccg ctgcacgctg gtcggtatcg aaataaccgc caatcagttt cggtaagatc
     2341 ccggcgatcc cttgtccaag catttgctgg aagaacagta acagaccgag cacccagacg
     2401 tttgcgccgc caatcgcaaa taccggaata atcagcagct gcgaggccag caggctacaa
     2461 acgtacgctt tgcgggttcc cagccagtca ccgaggaagc cacctacgca gcatcccacg
     2521 gccgcgccaa agccactaaa gaacagcaca ttggcgacag tatgcgggtt ataagccaga
     2581 tcggttttca gatacgttgg cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac
     2641 aacacgacca ccatcagcat tacgcccgtt ggccagcgtt tacccgtact ttgcaccata
     2701 aagctgataa agattgcggc gcataacagc ccgagaacag cgacgattgc agcattttgc
     2761 aggttaccgg cgaagcagaa ccacagcgca gtagccgccg ccagtgtcat tacgatattg
     2821 gcaatgcgat gttcaccacg gtagagaata tccaccattg tgcgtactgg tgctttacct
     2881 gcgtgtttct ctttccagtc ttccgcttcc gggatgtttt tacgcagcca gagagcaaag
     2941 atgattggca aaatgccgat aaagaacagc gcacgccaac cccagaccgg aaccaccagg
     3001 ctatagacct gagcggcaac gacggccccc acagagaagc ctgaaatcaa aaaaccactg
     3061 gctttgttac gcagatgttt tggccagctt tcaatgacat aggtggcgct ggaaccgtat
     3121 tcacccgcca tccccatgcc gatgaccaga cgagcgataa acatggtgat gtagcctggc
     3181 gcaaagccgc aggccagcgt cccggccgag aagagaacga tgctggtgac cattgccaga
     3241 cgacgcccgt agcggtcacc catagcgccg agcatcaggc cgccgaacca gcgagagata
     3301 aaggctgcgg agatcagact tgccgcctgc accgtcgtca gcccgaattc accctgtact
     3361 tcggtgagta ccagggcgat taaaacgaaa tcaaaaccgt caagcagata tcccaaccag
     3421 gcagcggaaa atgcgcgcca ttgtgcacgg ttgagatggc gataccacgg gatattctgg
     3481 gttgtagtac tcattgtgag tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa
     3541 attgtagggt acagatgcgt ttatttcccc tcacccggta ggggcgagcg aggggaaaac
     3601 aactcacccg cgctcttgca tcaactgctg ggccagcgcc ttcagttctg gcagatattt
     3661 ttcatctacc ggtccaaacg gtttgcggca cagcggcaca gaaacgacat ccatataatg
     3721 caggacagtt ttcaggccgc ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt
     3781 gcactcagtt tgcagtttct gcgcggtctg gatatcgcct tctttcagcg ccttcacgat
     3841 cccctgatat cgccagccca tgatgttgta agtactgccg ataccaccat cagcgcctgc
     3901 cagcaagccg gaagcgaaga tttcgtcgta accgttatag agcaccagat caggatgttc
     3961 acgacggatc tgttccatct gatagagatc gccagaagtc tgtttcagcg caccgacgcc
     4021 aggcaacgta accagggtgt tgatctgatc cagggtcagt tttaccccac tcagggctgg
     4081 aatgttgtac accaccatcg gcaaaccatc cgctgaatca ataattgccc gatagtgatc
     4141 gcagtgttct tcaaagctga aaggatagta gaacggcgtg acggcggaga cggcatcgaa
     4201 gccataacgt ttagccgatg ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac
     4261 gtgggcgatg agtttaatct tacctttcgc ctcttcggcg acgatttcca gtacctgttc
     4321 acgctcggaa aggctttgta caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc
     4381 gatgccctgc tgaatattga actgaaccag gcggcgcaga ctcgctttat ccagtgcttg
     4441 ttgttgatca aaaggagtca ggagtgcagc cattacgcca cgtaaattcg ttgccataaa
     4501 tacctctgaa gtgatgcttg tctgataaac gatatacctt tatacctgtt ataccagatc
     4561 aattaagcaa caccccatac agaaagctta taatgcgatc tgcttcacta aagtggcatt
     4621 atttcttttt gttggtggtc tgaccgaaag cgtgccaggt agcagagacg ctgttgagat
     4681 gcgattgcaa cgcacgatcg gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg
     4741 caatatgctg ttgataacta acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg
     4801 ggcgtgcggc aataagccag tcgagcaggg caacgtggat cgccatgaag attgggttac
     4861 cggggatctc cgccagcacg cggtggaaat caacgtctga acgaatgaat gccgcgttgt
     4921 tatccagcga ctgactgttg atttccagtg cttttgccag caaatcgatt tgctcatcgg
     4981 tggcgtgttc agccgcatag cgcaccaggc tggattcaaa gaacagacgt aattgttcga
     5041 aatgggcaat cccaccagga tgagaaagga aatctttcgc catgccggaa agctcgccga
     5101 tgatagtgtc cgcagaaggt cgagagacac gagcgcgttc gccgttgttt atttgcacca
     5161 gacctttgcg ttttaacgct gccagcgctt cacgcacaga aggacgcccg acgttaaaga
     5221 acgccatcag ttcgcgttca gacggtaatt gttcaccttc gccaaattca cgacggcgga
     5281 tcatctgttc cagctcttct tccaccattt cggagagttt tttacgcgcc agcgggcggc
     5341 tacgcaagtt gcgaccaatt gca
//
LOCUS       DAJTYH010000181         5271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTYH010000181 DAJTYH010000000
VERSION     DAJTYH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..737
                     /gene="celF"
                     /locus_tag="OO609_003858"
                     /gene_synonym="chbF"
     CDS             <1..737
                     /gene="celF"
                     /locus_tag="OO609_003858"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6635080.1"
                     /translation="IKDVLVNGKSRFAELLDGVASGQLKVSGVKNIFDLPFSEGLIRS
                     LNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKE
                     LEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCKLGRDGA
                     TPHPRITHFDDKVMGLIHTIKGFEIAASNAALSGEFNDVLLALNLSPLVHSDRDAELL
                     AREMILAHEKWLPNFADCIAELKKAH"
     gene            750..1508
                     /gene="chbG"
                     /locus_tag="OO609_003859"
     CDS             750..1508
                     /gene="chbG"
                     /locus_tag="OO609_003859"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6635081.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(1555..3816)
                     /gene="katE"
                     /locus_tag="OO609_003860"
     CDS             complement(1555..3816)
                     /gene="katE"
                     /locus_tag="OO609_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6635082.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            3999..4262
                     /gene="cedA"
                     /locus_tag="OO609_003861"
     CDS             3999..4262
                     /gene="cedA"
                     /locus_tag="OO609_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6635083.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            4539..5271
                     /locus_tag="OO609_003862"
                     /pseudo
     CDS             4539..5271
                     /locus_tag="OO609_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1338 a         1146 c         1339 g         1448 t
ORIGIN      
        1 tcattaagga tgtgctggta aatggcaagt cgcgctttgc cgaattgctt gatggtgtgg
       61 catccgggca gttaaaagta tctggcgtta aaaatatttt cgatctgcca tttagcgaag
      121 gcttaattcg ttctctgaat ctgttgccgt gttcttattt gctttattac ttcaagcaaa
      181 aagagatgct ggctattgaa atgggcgaat actacaaagg cggcgcacga gcgcaggtag
      241 tacagaaagt cgagaaacaa ctttttgagc tgtataaaaa cccggagttg aaagttaagc
      301 cgaaagaact ggaacagcgc ggtggggctt attactctga tgcagcgtgc gaagtgatca
      361 acgctatcta caacgacaag caagctgaac attacgttaa tatcccgcat catgggcata
      421 ttgataatat tccggcagac tgggcggtag aaatgacctg taagctgggg cgcgatggcg
      481 cgacgccaca tccgcgcatt acgcatttcg atgataaagt gatggggctg attcacacca
      541 ttaaaggctt cgagattgct gccagcaacg ccgcacttag cggagaattt aacgatgtgt
      601 tactggcgct aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac
      661 gcgagatgat tctggcgcac gagaaatggc tgccaaattt tgccgactgc atcgcagagc
      721 ttaaaaaagc acattaaccg aggctgatta tggaacgctt actgattgtt aatgccgatg
      781 attttggctt aagcaaaggt cagaactacg gcattatcga ggcctgtcgc aatgggattg
      841 tcacatcgac gacggcgctg gtgaatgggc aggctattga ccatgcggtg cagttgagtc
      901 gtgatgaacc gagtctggcc atagggatgc actttgtcct tactatgggc aagccactga
      961 cagctatgcc ggggttaacc cgcgatggtg tgctgggaaa atggatctgg cagttggcag
     1021 aagaagatgc tttaccgctg gaagaaatta ctcaggagct tgccagtcag tatttgcgtt
     1081 tcattgagct atttggacgc aaacctacgc atcttgatag ccatcatcat gtgcatatgt
     1141 tcccgcagat tttcccgatt gttgccaggt ttgcggctga agaggggatt gcgttgcgca
     1201 tcgaccgtca gccgctatct aacgctggtg atttaccggc taatctgcgc agttcgcagg
     1261 gattcagtag cgcgttttat ggtgaagaga ttagcgaggc actgttcctg caagtgctgg
     1321 atgacgcaag ccatcggggt gatccttcgc tggaggtgat gtgtcatccg gcgtttatcg
     1381 ataatactat ccgtcagagt gcttactgtt tcccacggtt aacggagctg gaagtgctga
     1441 catcagcgtc gttgaaatat gcaattgctg agcgtggtta tcggttgggt agttatcttg
     1501 atgtgtaagt aaggtggtaa ggtcactccc caggggacgt gaccttatcc atatttacgc
     1561 cggaattttg tcaatcttag gaatgcgtga ccacacgcgg tgttctgcca tcagcgttaa
     1621 cagttcatcc ataaaactac cgtcggtgct gtcagcttcc acaatccctt cttcaccctg
     1681 gtcagctacc ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg caatcggttt
     1741 aaggtgtttg taggcttcca tcagatagta gttggcatcg ccgttgtcag cgatatccgc
     1801 gatattgccg caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac cggcaaaggt
     1861 agcggctata ggcaacaccg taccgtcatc cgcagtcact tcacccattc gggagtagag
     1921 cagtttggca tgaacgcctt tggccttcat ggccttgaga atggccagaa ggtctgccga
     1981 tctcacttca tcattaagta aaatcgctac cacgcgacct ttcacatcac cgtcaggaat
     2041 ggcgtacaaa cttaaggatg gatccttttt cagaccgttg acgtccggag gtggggtgat
     2101 attcagctgg tcgtcagtca gttcgatacc gagatttttc gccaccgcct gggccagagt
     2161 gagatcaata tgcgccagct ggtcaacaac gcgctcacga atatacggac gaacgacttt
     2221 gcttaactca aaactgaaac catcgacaat atggcgctgc tcgaatggcg tctgacttag
     2281 ccagaacaga cgcggatggg aataatattc gccaaacgat gggctgcgct cgcgaacttt
     2341 attgccttcc acgcgctctt ggtatgattc aaaaccgccg cgtttcggcc ccggtggtgt
     2401 ttcgcgcggc cagttatcgt tgatcgagtt cggttcataa ttcgccgggt tagtgtcgat
     2461 ccccatacga tgcatgccgt cacgctggaa attatggtaa gggcaggtcg ggcggttaat
     2521 cggaatctca tggaaattcg gcccaccaag acgactgatt tgtgtatcgg tataggagaa
     2581 caaacgtccc tgcaacaggg gatcgttggt gaagtccaga ccaggaacaa tatgacctgg
     2641 atggaatgct gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat tgagcaccat
     2701 tttgccgaca cgctgaacgg gcaccagttc ttccgggatc agtttggttg gatcgagaag
     2761 atcgaagtcg aacttgaatt catcttcttc aggaatcaac tggaagccca gttcgtattc
     2821 cggaaaatcg cctgcttcaa tggcttccca caactcgcgg cggtggaagt ccgggtcacg
     2881 tccggtgagt ttttgtgctt catcccaaac gagtgaggct ttacctgcca gtggtttcca
     2941 gtggaaacgt acaaacgttg ccttcccttc ggcattaatc aggcggaagg tgtgaatacc
     3001 gaagccttcc atggtgcggt aactgcgggg gatgccgcga tccgacatcg cccacatcac
     3061 gttgtgcaga gtttcaggtt gcagagaaac ataatcccag aaagtatcgt gggcactttg
     3121 cccttgtgga attgcccagt gcggttctgg ttttaccgca tgaacaaaat cggggaattt
     3181 atgcgcatcc tggataaaga agattggcgt gttattgcca acgaggtcaa aaataccctc
     3241 ttcagtatag aacttggtgg caaaaccacg gatatcacgc acggtatcgg cagagccagc
     3301 gccaccctga acggtagaga acctaacaaa tactggggtg attttgttcg gatctgagag
     3361 gaaatccgct ttggtgatat cgcttaagct tttatatggc tggaaataac cgtgagctgc
     3421 tgagccgcgt gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt gggtgatttt
     3481 ctcgcgcaga ataaaatctt ccagcagcgt tggaccacgg ttaccggcac gcagtgagtt
     3541 ttgatcatcg gcgatgcgca cgccctgatt agtggtcagc gcataatttt cactgccttt
     3601 gcgtacgtct tccagagaat taagtttttc gttacgtgta tcaggggctt tcaggctccc
     3661 aggggcggta ggttgtgcac ctggcggtgt tggttcagcc gctggacgat gagatccgtc
     3721 ctcaggtgcc agtgagtcca tccccggttt cgcttcgctg gaatcgtgta gtggtgactg
     3781 gtgctgatgt gggttctttt cgttatgttg cgacattgaa ctcgtctcct taatttatta
     3841 ctgaaagggc cgctgttttg gtcaaaaaac tctctaacta tagaccacca gccagatccc
     3901 gcttcggaga ctattctgga aacggtgtta tgaaatacac gcaaagcggg acgtacaggg
     3961 gctaaatcgg ctaaactaca gtttttctaa tttttaatat gcgtttagtg aagccagtaa
     4021 tgaagaaacc gctccgccag caaaaccgcc agattattag ctatgtccca cgcacggaac
     4081 ccgcgccgcc agaacatgcg ataaagatgg attcgtttcg tgatgtctgg atgctgcgtg
     4141 gcaaatatgt tgcgtttgta ctgatgggag agtcatttct gcgctcaccg gcgtttactg
     4201 tgcctgaatc tgcccaacgt tgggcaaatc agatccgcca ggaaggggaa gtgactgagt
     4261 aaggattaca tacctcccgg ttttgccggg agttacagga cctgaaattc tgcatttcgt
     4321 tgctacattc attcaaacca cctgtaacaa aaagctgcct ttgacgagct attgctataa
     4381 ataagagatc ttttttcttt aattatttat ttattttaaa atggttaagt tttgctaatc
     4441 atgatgagtt atttttgata gttcatcctg tggcgtcgct cttaaactta tattgtaaat
     4501 gacttagatt taaataagat aataattttg ttaacaatat gaggcgggag atgttttcta
     4561 caaacttaat acagagtaat tatggtgatt taaatattaa aagcctggct ttcgactctt
     4621 ttaaagaaag gcttgagtct accatggcgg cactcacttt ctttatatca acgggtcaat
     4681 gtgactgcga tgaaatagca gagtcgaact tcaatcgtat gattgcctat atgtctaaca
     4741 ttaactatga tgcctcaaag ctaggagcgc cagcacttag ttttgatacg tatctgcagg
     4801 acaatgttaa atatagagtg ataattaata atctttatgg cagtgagttt cggataagag
     4861 atattaatga gaattttgtt gggatggatt ttacctgtga attccgaccc gaaaaaatga
     4921 cgaatttgat caatataaaa aataggttgg ttattcatta tttaaggact atatactatg
     4981 aaaaaaatta tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt
     5041 ctcctaaagt ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta
     5101 aatcttccgg gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca
     5161 cttagaaaac gtctcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc
     5221 caggaatgta ttgataagtt ggacattttt ttcgctgacg atctgtttaa a
//
LOCUS       DAJTYH010000182         5265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTYH010000182 DAJTYH010000000
VERSION     DAJTYH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..363
                     /locus_tag="OO609_003863"
     CDS             <1..363
                     /locus_tag="OO609_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR6635084.1"
                     /translation="IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAK
                     QSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEH
                     SAHEGMEGMDMSHAESAH"
     gene            385..2376
                     /gene="cyoB"
                     /locus_tag="OO609_003864"
                     /pseudo
     CDS             385..2376
                     /gene="cyoB"
                     /locus_tag="OO609_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2366..2980
                     /locus_tag="OO609_003865"
     CDS             2366..2980
                     /locus_tag="OO609_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6635085.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            2980..3309
                     /locus_tag="OO609_003866"
     CDS             2980..3309
                     /locus_tag="OO609_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6635086.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3321..4211
                     /gene="cyoE"
                     /locus_tag="OO609_003867"
     CDS             3321..4211
                     /gene="cyoE"
                     /locus_tag="OO609_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6635087.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4395..4706
                     /locus_tag="OO609_003868"
     CDS             4395..4706
                     /locus_tag="OO609_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6635088.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4690..4848
                     /locus_tag="OO609_003869"
     CDS             4690..4848
                     /locus_tag="OO609_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6635089.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4841..5239
                     /locus_tag="OO609_003870"
     CDS             4841..5239
                     /locus_tag="OO609_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6635090.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1151 a         1303 c         1395 g         1416 t
ORIGIN      
        1 atcgccaacg aacccggtac ttatgacggt atctccgcca gctacagcgg gccgggcttc
       61 tcaggcatga agttcaaagc tattgcaaca ccggatcgcg ccgaattcga ccaatgggtc
      121 gcaaaagcga aacagtcgcc gaacaccatg tctgacatgg ctgcgttcga aaaactggcc
      181 gcgcctagcg aatacaacca ggtggaatat ttctccaacg tgaaaccaga cttgtttgcc
      241 gatgtgatta acaagtttat ggctcacggt aagagcatgg acatgaccca gccagaaggt
      301 gagcacagtg cacacgaagg tatggaaggc atggacatga gccacgcgga atccgcccat
      361 taaaggggtt gaggaagaat aaagatgttc ggaaaattat cacttgatgc agtcccgttc
      421 catgaaccta tcgtcatggt tacgatcgct ggcattattt tgggaggtct ggcgctcgtt
      481 ggcctgatca cttacttcgg taagtggacc tacctgtgga aagagtggct gacctccgtc
      541 gaccataaac gcctcggtat catgtatatc atcgtggcga ttgtgatgtt gctgcgtggt
      601 tttgctgacg ccattatgat gcgtagccag caggctcttg cctcggcggg tgaagcgggc
      661 ttcctgccac ctcaccacta cgatcagatc ttcaccgcgc acggcgtgat tatgatcttc
      721 ttcgtggcga tgcctttcgt tatcggtctg atgaacctgg tggttccgct gcagatcggc
      781 gcgcgtgacg ttgcgttccc gttcctcaac aacttaagct tctggtttac tgttgttggc
      841 gtgattctgg ttaacgtttc tctcggcgtg ggcgaatttg cgcagaccgg ctggctggcc
      901 tatccaccgc tatcgggaat agagtacagt ccgggagtcg gtgttgatta ctggatatgg
      961 agtctccagc tatccggtat aggtacgacg cttaccggta tcaacttctt cgttaccatt
     1021 ctgaagatgc gcgcaccggg catgaccatg ttcaagatac cagtatttac ctaggcatca
     1081 ctgtgcgcga acgtactgat tattgcttcc ttcccaattc tgacggttac cgtcgcgttg
     1141 ttgaccctgg atcgctatct gggcacccat ttctttacca acgatatggg tggcaacatg
     1201 atgatgtaca tcaacctgat ttgggcctgg ggccacccgg aagtttacat cctgatcctg
     1261 cctgttttcg gtgtgttctc cgaaattgcg gcaaccttct cgcgtaaacg tctgtttggt
     1321 tatacctcgc tggtatgggc aaccgtctgc atcaccgtgc tgtcgttcat cgtttggctg
     1381 caccacttct ttacgatggg tgcgggcgcg aacgtaaacg ccttctttgg tatcaccaca
     1441 atgattatcg ccatcccgac cggggtgaag atcttcaact ggctgttcac catgtatcag
     1501 ggccgcatcg tgttccattc tgcgatgctg tggactatcg gttttatcgt caccttctcg
     1561 gtgggcggta tgactggcgt gctgctggca gtaccgggcg cggacttcgt tctgcataac
     1621 agcctgttcc tgattgcgca cttccataac gtgatcatcg gcggcgtggt cttcggctgc
     1681 ttcgcaggga tgacctactg gtggcctaaa gcgttcggtt tcaaactgaa cgaaacctgg
     1741 ggtaaacgcg cgttctggtt ctggatcatc ggcttcttcg ttgcctttat gccgctgtat
     1801 gcgctgggct tcatgggcat gacccgtcgt ttgagccagc agattgaccc gcagttccac
     1861 accatgctga tgattgcagc cagcggtgca gtactgattg cgctgggtat tctctgcctc
     1921 gttattcaga tgtacgtttc tattcgcgac cgcgaccaga accgtgacct gactggcgac
     1981 ccgtggggtg gccgtacgct ggagtgggca acctcttccc cgcctccgtt ctataacttt
     2041 gccgtagtgc cgcacgttca cgaacgtgat gcattctggg aaatgaaaga gaaaggcgaa
     2101 gcgtataaaa agcctgacca ctatgaagaa attcatatgc cgaaaaacag cggtgcaggt
     2161 atcgtcattg cagctttctc caccatcttc ggtttcgcca tgatctggca tatctggtgg
     2221 ctggcgattg ttggcttcgc aggcatgatc atcacctgga tcgtgaaaag cttcgacgag
     2281 gacgtggatt actacgtgcc ggtggcagaa atcgaaaaac tggaaaacca gcatttcgat
     2341 gagattacta aggcagggct gaaaaatggc aactgatact ttgacgcacg cgactgccca
     2401 cgcgcacgaa cacgggcacc acgatgcagg cggaaccaaa atcttcggat tttggatcta
     2461 cctgatgagc gactgcattc tgttctcaat cttgtttgct acctatgccg ttctggtgaa
     2521 cggcaccgca ggcggcccga caggtaagga cattttcgaa ctgccgttcg ttctggttga
     2581 aactttcttg ctgttgttca gctccatcac ctacggcatg gcggctatcg ccatgtacaa
     2641 aaacaacaaa agccaggtta tctcctggct ggcgttgacc tggttgtttg gtgccggatt
     2701 tatcgggatg gaaatctatg aattccatca cctgattgtt aacggcatgg ggccggatcg
     2761 cagcggcttc ctgtcagcgt tctttgcgct ggtcggcacg cacggtctgc acgtcacttc
     2821 tggtcttatc tggatggcgg tgctgatggt gcaaatcgcc cgtcgcggcc tgaccagcac
     2881 taaccgtacc cgcatcatgt gcctgagcct gttctggcac ttcctggatg tggtttggat
     2941 ctgtgtgttc actgttgttt atctgatggg ggcgatgtaa tgagtcattc taccgatcac
     3001 agcggcgcgt cccatggcag cgtaaaaacc tacatgacag gctttatcct gtcgatcatt
     3061 ctgacggtga ttccgttctg gatggtgatg acaggggctg cctctccggc cgtaattctg
     3121 ggaacaatcc tggcaatggc agtggtacag gttctggtgc atctggtgtg cttcctgcac
     3181 atgaatacca aatcagatga aggctggaac atgacggcgt ttgtcttcac cgtgctaatc
     3241 atcgctatcc tggttgtagg ctccatctgg attatgtgga acctcaacta caacatgatg
     3301 atgcactaag agcggcggtt atgatgttta agcaatacct gcaagtaacg aaaccaggca
     3361 tcatctttgg caacctgatc tcggtgattg ggggattcct gctggcctca aagggcagca
     3421 ttgattatcc cctgtttatc tacacgctgg ttggggtgtc actggttgtg gcgtcgggtt
     3481 gtgtgtttaa caactacatc gacagggata tcgacagaaa gatggaaagg acgaagaatc
     3541 gggtgctggt taaaggcctg atctctcctg ctgtctcgct ggtgtacgcc accttgctgg
     3601 gtattgctgg ctttatgctg ctgtggtttg gcgcgaatcc gctggcctgc tggctggggg
     3661 tgatgggctt tgtggtttat gtcggcgtct atagcctgta catgaaacgc cactcagtct
     3721 acggcacatt gattggttcg ctctccggcg ctgcgccgtc ggtgattggc tactgtgcag
     3781 taaccggcga gtttgatagc ggcgcagcga tcctgctggc tatcttcagc ctgtggcaga
     3841 tgcctcactc ctatgccatc gccattttcc gctttaagga ttaccaggcg gcaaacattc
     3901 cggtattgcc agtggtaaaa ggcatttcgg tggcgaagaa tcacatcacg ctgtatatca
     3961 tcgcctttgc cgttgccacg ctgatgctct ctcttggcgg ttacgctggg tataaatatc
     4021 tggtggtcgc cgcggcggtt agcgtctggt ggttaggtat ggctctgcgc ggttataaag
     4081 ttgctgatga cagaatctgg gcgcgcaagc tgttcggctt ctctatcatc gccatcactg
     4141 ccctctcggt gatgatgtcc gttgatttta tggtaccgga ctcgcatacg ctgctggctg
     4201 ctgtgtggta acaaaacctc tctattaaaa aggtgctacg gcaccttttt attgtctgag
     4261 atcagagata cggctaataa ccgtatctct tgacagagtg agcagatgca cataaattaa
     4321 tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc attcagttaa
     4381 tcaaagaagt cataatgcct ggaaaaacag ccgctctata cgatgtcgat aaaacgctga
     4441 aaaatgcgcg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt cgtgaagctg
     4501 cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgctgctatg gaacgcgccg
     4561 aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg gaactgatga
     4621 accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta atgaaaagga
     4681 aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct ctcctgtaat
     4741 tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa ccctgttatc
     4801 gataaatttg tacgcgctac gcgcatcgct aaagaaaagt gtgcgtaata gcgactgtgc
     4861 ggctaaagca cttattgaca gacaaagtgg tgaactgatt ggcatctgta cttttacggc
     4921 atattcgctg gaaagacaac gcgtttctgg cgtccttcag ggttcacaac cttcagaaat
     4981 tggtgttgtc agattagtca tgttgggggg agcgcggaag tatcaaaagc ggggctttgg
     5041 tcaggaccta ctatgtgatt tttttgaaca tgtaaaaaaa attcacccgg cattaccaat
     5101 taaaggggtt tatcttgatg ctgaccctgc cgccattaac ttttatgctc gtctcggctt
     5161 tgttcagctt tcagcgacac caaatgcttt tggtgctgta cctatgtttt tggcgattca
     5221 gcatattctc gcggcttaga ttattatcgc cacccgccag ggcaa
//
LOCUS       DAJTYH010000183         5129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTYH010000183 DAJTYH010000000
VERSION     DAJTYH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(56..997)
                     /locus_tag="OO609_003871"
     CDS             complement(56..997)
                     /locus_tag="OO609_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6635091.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1009..1498)
                     /locus_tag="OO609_003872"
                     /pseudo
     CDS             complement(1009..1498)
                     /locus_tag="OO609_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1502..2722)
                     /locus_tag="OO609_003873"
                     /pseudo
     CDS             complement(1502..2722)
                     /locus_tag="OO609_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
     gene            complement(2726..3469)
                     /locus_tag="OO609_003874"
     CDS             complement(2726..3469)
                     /locus_tag="OO609_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6635092.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3360..4826)
                     /locus_tag="OO609_003875"
     CDS             complement(3360..4826)
                     /locus_tag="OO609_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6635093.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTAMGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4826..5059)
                     /locus_tag="OO609_003876"
     CDS             complement(4826..5059)
                     /locus_tag="OO609_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6635094.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
BASE COUNT         1088 a         1447 c         1294 g         1300 t
ORIGIN      
        1 tcattttttg ccatcgggtc atccttaatg gccccgccag ggggccatgt ggctgttaaa
       61 tgccatcaac gtacgaaatg gtttctttgt acactggctc gactgcaccc agcttgccgt
      121 agtaagtgac gatctgatac agaccgcgat actgcaccgg cacgctctga agcggaacca
      181 gcgggtagcg gacgtatttt ttatcgttgg tgtacgcaac tatgcgatcc ttattcccca
      241 caccacggcc tttcagccat ttaaccgcgc ggatattcag cggaacaccg ttctggtgat
      301 agctgatggt gttggtctga aggttcgtca acagggactg gttacctgca gatgaaacga
      361 tgatgctgga caacagagca aactgctcag gcgggatcag caaatcacgc ggaaccacag
      421 agtaaccaga agcggcccac gcatcagaca gcacctggtt aatgcttgcg cggatttcgt
      481 ccggtgttga ggttgcccac gttttggcag cgttgttgac aggcacgccg tccagggtaa
      541 caaggccttt caggtttaat gcggaatcgc caacatatac ctgttcatcg ttatccatct
      601 gccatttcag ttgcataccg tcatacttct gcgtatcaat cgggcggccg acctgctgag
      661 cagcctgcaa ttctatgacc gtccagccaa gttccatccc ccacaggttc agcgggttac
      721 cggatttgcc gatatccacg ttcacgccag caatagcggt tgagtctttg cctacccagt
      781 tttttccatt cggatttgcg ccagtacccg cagcggcgaa gctggtattc gtccagctgg
      841 aaatgtcatc tgcgatggag gcgtcttcac gcagttgaat atcgcggctc caggtgtacc
      901 ccaccagtgg caggttcagc gtctggtcga gtctctccag ctccccgatg agaaaggcac
      961 cagagctgtc aacggttgcc tgatcaaaag taatcattcg tctgttcctt aaatcttcca
     1021 ggaaatttct gcattgccgt cagcatcacc ggcacctgtg aattcagcgt tggtcagcac
     1081 cacgtttttg ccactgactg acgtggacat gaatccaccc agaggcactt tgatggattc
     1141 atcagtggag atcgcctttt ttgatagtgc tggcatcaaa atcagaaccg agattaacgg
     1201 tcacgtagcc acgcttcatg gcgtcgcccg ggaagttctt gcctgttccc acctggcgaa
     1261 ccatgtccgg ctgcgacgtg gtcggataag ggcgcacgta gatccccttc accttgtctg
     1321 cggtatcacc atctgccagc ggcacgaaaa aaccgtcatc atcgtattta ccagccaggc
     1381 cataggcagc gaaggcgtta tcggatttaa ggaccaccgg ttcgacggtt aagtcctgcg
     1441 ggcgagagac agccccggca atgccaacag gcatccggta cagaaataca ttattcattt
     1501 tttacccttt acggtttgcc cagaattcag cgttttgttt gttcagggaa gcgatactgg
     1561 tcatgcccat gtttaggcgc tgtgcatcgc cggtggtggc gcgggtgttt cgccctttgg
     1621 caacctcaga cacggcatta aacgccatgt cgaccgattg tttcggtaat ttgcggatat
     1681 ccgcatcacc gactatctgg cgaaccagcg ttttgtcagc agaagccaga acctcgcgtt
     1741 tgaacgcggt cggtttcatc ttacggctca gatcgatacc cggaacgata acttcggcac
     1801 gccaggctga gtcaccagta atcgtggttt cctcttcatc gtcctcgccg tcaccggtcg
     1861 gattatcgtc aggcttatta tcgttatcgc ccgtggcatt tctttccagc ttagccagca
     1921 gggctttcag taatgttttg aggtcatcat cactgtcgcc ggttggatct ccgcccatct
     1981 ctggtgcttt gtccggtagt ggttgctgcg gggacaggtt gatgttgaga ttaacgccct
     2041 gcggcaaatc cccctaatct cctgtaaccg atgcgggagc cgactccacc agttcgttca
     2101 tggtgtcggc atctcctgtc ttgatggccg cacgcatgcg gttccaccag tttttctttt
     2161 gatttgccat tgtgtctctg tctccaattg cacaacgatt tccggctctg cctttaggga
     2221 caagagccac atggtttccg gtaatatcga cctgctcggc tttacctggc tcggtctgct
     2281 cgtactccgc gtcatagccg cacgacactt cgcgcaggcc atcttcgata agctgaatgg
     2341 cgctttcgtc tttgacgata aggtcagcca gcatcaaatc agactgctca cccgtcccgc
     2401 gccggacatt ctggaggtgc ccgacagcaa gcactttcca gttctcggga tttaccagcc
     2461 gcacattccc gttttcatct tcaggatgca ggatcgtgat gctcatccct tcgaatgagg
     2521 caagcgtggc cggatggaat acctgctcag gagaacgcgt gacgactatt tcaacgaact
     2581 tatcgggttt cagttttggc aggtcatcag caccatagag ctgcttacct gttcgtccta
     2641 tcggcacgtc tttgcacagc aacgagccgt cagccaggcg gtatcgggtt tccccgagcc
     2701 tggtctcaaa aaagtatttc atgggttatg ctgccctttt cattgcgaat ggaacgcgaa
     2761 caaacacgat ctctttatag cagcggcagt ttggcagctc gcccgcgtgg ccggtcatgc
     2821 cgtccaaagt tggaggtgaa tcccagcgga cgaatttacc tttcatgtga tcgtgggaat
     2881 ctcggacatc gccatcatcg gctgtacgcc aaatataacc atccgagcca atagctatcg
     2941 ctcgggccat atccagcgcg cccgttgctc ttcccagttc cgttcgggca atcagatcgg
     3001 ccctagactt agcaacatcg ccggtagatg caatctcttt agcaaactgc tccgcccgac
     3061 caccggtgac aacagcctct atcgctttgt tctggatgtc gtacaccctg tctgcggcct
     3121 cgagaggcag agatttgatg tacttgacct gttcggcgat gatggattgc atcacctggc
     3181 ccacaggagc gctttccaca agattgcgga gctcgcgact gatgttcttg ctgtgttgcc
     3241 gccaaacttt ctcgttctgc cgggttaggt ccgcagtaaa gttttccgcg acctttgtcg
     3301 cccagggggt gatgatttca ctgtaacgct ccagcgccgc aataatttcc gtgatactgt
     3361 catttgaacc atcgtagcga ccatttacga tgtctccgac cgccctcgct atcctgcgta
     3421 ggctggttcg ataccggatt tccgcctgac ggctcctgcg gttcgtcatc aggttcgccg
     3481 atgccgggcg gcgcttcatc ttcggcattc tcgatgtcct cgttggtaat ggatgcccct
     3541 atgccggtta cgtcagaatt ttctcgcaaa tcagtcatag cggctttcag tgtcatcaga
     3601 ccatcaccca tcgccgtact gattgcgttg gtggtgttta acgccaccgt tgagcgatcg
     3661 acatcagaca tttgccagag cgggttaaac tcaaacgtga aatcgtccgg cagcggcttg
     3721 ccaagttccg aacgatgcat gatgtccagt atccgccgca ccggaagacg taaacgcctc
     3781 tcctgcaacg aactgatgcg atcgtaatag ttggcaaggt ctgcatcacc ggtagaaaat
     3841 cctttcgggg actgtccgaa caaccgcacc agtgggatac caacagcgcc actaatctgt
     3901 tctgcaaact gcgaaaggat gtcatccaga ccactgaagc tgtactgatg ggtttcaaac
     3961 ttatcccgcg agtccatgag cgtcatgcct tcattactct ggaactgtcg aatcaggtcg
     4021 atattcttca gcaacgcttc atacgcagga ccaccaagtg cgataagctc gcgtagcttc
     4081 tccacgctgt aggtacgcaa atgcgccttg tagaccagct gcgccgcgcc gacagtagcg
     4141 ctgtcgaacg cggtaagacg atcccagata cgctctacaa ccgacattcc ccattcgttc
     4201 tcggtcatct tctgctgaaa tggcaacgtg acgccatcaa agcgaatcag gcgactgtga
     4261 tgaatgcgcc aggcaggaat tcccgttgct gtggtcacca catcgtaaaa ctcaggttta
     4321 cccaggtccg gccccatatc tttaatgcgg cgggtcagtg ccgggtcgac catccagcgg
     4381 tcgagcggga gaatcccctt aaacttgccc ttaccgatgg tttcgggtcg cagcggggtc
     4441 attggtgcct gcccctcaat catgatgaaa cccaccgcgc cgccgtagag gcgcgaccat
     4501 ttcagcacgt cattcagcgc atcccagatt tgcaactcat ccagttgtga ttcgagaatg
     4561 ccacgatctt ttgcatcaat ttccgaagtg atgcgaatgc ctttgcgggt catatcatcc
     4621 gggatagcat cgactgcttc gccgatgatc caggatgaac gataggacca ttccaccagc
     4681 atgcggttac gactggtgaa attagcccgg taggtggatg ctgagtgctg gttaggtgtc
     4741 tgcatcccta ctcgggcaat aaaattctca taaccatcag ctgtggcctg cacagttcgc
     4801 cgcagggctt gtttgtttcg tgccatcagg cctgtctccc cagctgttcc cagatatcca
     4861 gcgatgtatc gattggcgcg aaggccataa tgaatgcatc agcaacgttt ggtgacggta
     4921 tctcgcgttt tgcgaggtct tttttacttt cgaccatcac acgtccgtta cggtcgaaat
     4981 cacgatgagg tgttgtcagt tccagtttca gcttttcaag caacggacaa cgagaatcta
     5041 tgctgatcag ctcatccaca ggatactgtt ctccgttgtt aatggcgtta aacgtatttc
     5101 tgaaacggtc agccaccatg cctgagcct
//
LOCUS       DAJTYH010000184         5106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTYH010000184 DAJTYH010000000
VERSION     DAJTYH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..921
                     /gene="tauA"
                     /locus_tag="OO609_003877"
     CDS             <1..921
                     /gene="tauA"
                     /locus_tag="OO609_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6635095.1"
                     /translation="FIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWRKF
                     DSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTISK
                     PEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAY
                     VWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQ
                     QPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAI
                     IDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            934..1701
                     /gene="tauB"
                     /locus_tag="OO609_003878"
     CDS             934..1701
                     /gene="tauB"
                     /locus_tag="OO609_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6635096.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1698..2525
                     /gene="tauC"
                     /locus_tag="OO609_003879"
     CDS             1698..2525
                     /gene="tauC"
                     /locus_tag="OO609_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6635097.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2522..3373
                     /gene="tauD"
                     /locus_tag="OO609_003880"
     CDS             2522..3373
                     /gene="tauD"
                     /locus_tag="OO609_003880"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6635098.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3479..4453)
                     /gene="hemB"
                     /locus_tag="OO609_003881"
     CDS             complement(3479..4453)
                     /gene="hemB"
                     /locus_tag="OO609_003881"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6635099.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1173 a         1282 c         1435 g         1216 t
ORIGIN      
        1 ttcatcgctt ttcaggcaca ggcggtgaac gtcaccgtgg cgtatcaaac ctcagccgaa
       61 ccggcgaaag tggctcaggc cgacaacacc tttgctaaag agagcggggc gagcgtggac
      121 tggcgtaagt ttgacagcgg agccagcatc gtgcgggcgc tggcttctgg cgacgtgcaa
      181 atcggcaacc tcggttccag cccattagcg gttgcagcca gccaacaggt gccgattgaa
      241 gtcttcttgc tggcgtcaaa actgggtaac tccgaagcgc tggtggtaaa gaaaactatc
      301 agcaaacccg aagatttgat tggtaaacgc attgccgtac cgtttatctc caccacccac
      361 tacagcctgc tggcggcgct gaaacactgg gggattaaac cagggctagt ggagattgtg
      421 aacctgcagc cgcccgcgat tatcgccgca tggcagcggg gtgatattga tggtgcttat
      481 gtctgggcac cggcggttaa cgccctggaa aaagacggca aggtgctgac cgattctgaa
      541 caggtcgggc agtggggtgc gccaacgctg gacgtctggg tggtgcgcaa agattttgcc
      601 gagaaacatc ctgaggtcgt gaaagcgttc gccaaaagcg ccatcgatgc acagcaaccg
      661 tacattgcta accctgacgc gtggctgaaa cagccggaaa acatcagcaa actggcgcgt
      721 ttaagcggcg tgcctgaagg tgacgttccg gggctggtga agggaaatac ctatctgacg
      781 ccgcagcaac aaacggcaga actgaccgga ccggtgaaca aagcaatcat cgacaccgcg
      841 cagtttttga aagagcaggg caaggtcccg gctgtagcga atgattacag ccagtacgtt
      901 acctcgcgct tcgtgcaata aaaggaggcg cagatgctgc aaatctctca tctctatgcc
      961 gattatggcg gcaaaccggc actggaagat atcaacctga cgctggaaag cggcgagcta
     1021 ctggtggtgc tggggccgtc cggctgcggt aaaaccaccc tgctgaatct gattgccggt
     1081 tttgtgcctt atcagcatgg cagcattcaa ctggcgggta agcgtattga gggaccggga
     1141 gcagagcgtg gcgtagtttt tcagaatgaa gggctgctac cgtggcgcaa tgtacaggac
     1201 aacgtggcgt tcggcctgca attggcaggt atagagaaaa tgcagcgact ggaaatcgcg
     1261 caccagatgc tgaaaaaagt ggggctggaa ggcgcagaaa aacgctatat ctggcagctt
     1321 tccgatggtc aacgtcagcg ggtggggatt gcccgcgcgc tggcggcgaa tccccagctg
     1381 ttattactcg acgaaccgtt tggtgcgctg gacgccttca cccgcgacca gatgcaaact
     1441 ctgctgctga aactctggca ggagacgggc aagcaggtgc tgttgattac ccacgatata
     1501 gaagaagcgg tgtttatggc gactgaactg gttctgcttt catccggccc tggccgtgtg
     1561 ctggagcggc tgcggctcaa ctttgctcgc cgctttgttg cgggagagtc gagccgcagc
     1621 atcaagtccg atccacaatt catcgccatg cgcgaatatg ttttaagccg cgtatttgag
     1681 caacgggagg cgttctcatg agtgtgctca ttaatgaaaa actgcattcg cggcggctga
     1741 aatggcgctg gccgctctcg cgtcaggtga ccttaagcat tggcacgtta gcggttttac
     1801 tcactgtatg gtggacggtg gcggcgctgc aactgattag cccgctgttt ttgccgccgc
     1861 cgcaacaagt actggcaaaa ctgctcacca ttgccggacc gcagggattt atggacgcca
     1921 cgctgtggca gcatctggca gccagcctga cgcgcattgt gctggcgcta ctcgcggcgg
     1981 tggtgatcgg tattccggtc gggatcgcca tggggctaag cccgacggtg cgcggcattc
     2041 tggatccggt aatcgaactt tatcgtccgg tgccaccgct ggcttatttg ccgctgatgg
     2101 tgatctggtt tggtattggt gaaacctcga agatcttact gatctattta gcgatttttg
     2161 cgccggtggc gatgtcggcg ctggcagggg tgaaaagcgc gcagcaggtt cgcattcgtg
     2221 ccgcccagtc gctgggtgcc agccgtgcgc aggtgctgtg gtttgtcatt ttgcccggcg
     2281 cgctgccgga aatcctcacc ggattacgca ttggtctggg tgtgggctgg tctacgctgg
     2341 tagcggcgga gctgattgcc gcgacacgcg gtttaggatt tatggttcag tcagcgggtg
     2401 aatttctcgc gactgacgtg gtactggcgg ggatcgcggt gattgcgatt atcgcctttc
     2461 ttttagaact gggtctgcgc gcgttacagc gccgtctgac gccctggcat ggagaagtac
     2521 aatgagtgaa cgtctgagca ttaccccgct ggggccgtat atcggcgcac aaatttcggg
     2581 tgccgacctg acgcgcccgt taagcgataa tcagtttgaa cagctttacc atgcggtgct
     2641 gcgccatcag gtggtatttc tgcgcgatca agctattacg ccgcagcagc aacgcgcgct
     2701 ggcccagcgt tttggcgaac tgcatattca ccctgtttac cctcatgccg aaggggttga
     2761 cgagatcatc gtgctggata cccataacga taatccgccg gataacgaca actggcatac
     2821 cgatgtgaca tttattgaaa cgccacccgc aggggcgatt ctggcagcta aagagttacc
     2881 ttcgaccggc ggtgatacgc tctggaccag cggtattgcg gcctatgagg cgctctctgt
     2941 tcccttccgc cagctgctga gctggctgcg tgcggagcat gatttccgta aatcattccc
     3001 ggaatacaaa taccgcaaaa ccgaggagga acatcaacgc tggcgcgagg cggtcgcgaa
     3061 aaatcctccg ttgctacatc cggtggtgcg aacgcatccg gtgagcggta aacaggcgct
     3121 gtttgtgaat gaaggcttta ctacgcgaat tgtcgatgtc agcgagaaag agagcgaagc
     3181 cttgttaggc tttttattcg cccatatcac taaaccggag tttcaggtgc gttggcgctg
     3241 gcaaccgaat gatattgcga tttgggataa ccgcgtgact cagcactatg ccaatgccga
     3301 ttacctgcca cagcggcgga ttatgcatcg ggcgacgatc cttggggata aaccgtttta
     3361 tcgggcgggg tgatacgagt gcggacggtt ccctcgcccc cttggggaga gggttagggt
     3421 gagggggcgt tcactgtact ttcaacaggt taactccccc tttctgagag gaaaaaaatt
     3481 aacgcagaat cttcttctca gccaaatcca gcgcaaagta gctgaaaatc agatccgcac
     3541 ccgcacgctt aattgaacct aagctttcga gcacgacttt ctcttcatct atagcacccg
     3601 ccagcgcggc gaacttaatc atcgcgtact caccgctcac ctgatacgcg ccaatcggca
     3661 attcagtacg ttcacgcagc tcacgcacga tgtcgaggta cgctccagca ggtttaacca
     3721 tcaggcagtc tgcgccctgg gcttcatcca gcagtgactc acgaatcgcc tcgcgacggt
     3781 tcattgggtt catctgatag cttttgcggt cgccttttaa tgcgcttcct gcagcttcac
     3841 ggaacgggcc ataaaaggaa gaggcgaact tggtcgaata cgacataatc gccgtatctt
     3901 taaagcctgc agcgtccagc gcctgacgaa tcgcctgtac ctggccgtcc atcgcggcgg
     3961 aaggggcgat gaagtctgcg cctgcagcag ctgcaaccac ggcttgcttg cctaaatttt
     4021 ccagagtcgc gtcgttgtcg acgccatgct cgcacagcac accgcagtga ccgtgagaag
     4081 tgtattcaca gaaacaagtg tctgacatga cgatcatttc tggcacagtc tgcttgcaga
     4141 tgcgcgacat acgcgccacc agtccatctt cccgccaggc gtcgctgccg gtttcatcgg
     4201 tatggtgaga gatgccaaaa gtcattacgg aacggatacc ggcgttggcg atgcgttcaa
     4261 tttcgcgtgc cagatgtttc tctggaatgc gcatcacgcc tggcatggct tcaacggctt
     4321 tgtagtcgtc aatttcttct tcaacaaaga tcggcaacac caggtcgtta aggctaagtg
     4381 ttgtctcttc aaacatagcg cgcagcgcag gagatttgcg caggcgacga gggcgttgga
     4441 ttaagtctgt catggtctgc ctgatgtttg tggaattaag gtgcacagta tacctgaagc
     4501 ggggcaggag tgttttacga aagttgtcgc gatgttgaca agaagagaat gaaagagaga
     4561 ccaggacatc tttgtaatag aaatagcaat aatttcatat tcactgaaaa tatttttaat
     4621 ctttatttat agctcttggt tattattttt tggcgtttgg ttgtgctgct ataaattgaa
     4681 taattcaaac tttgttgtat attgataaga tatttatctg gtttgtattt ttgatattta
     4741 taacgcgtat gcatcaaaaa taacattatc tgcaacatac tgaaatatcg tccttttatc
     4801 aatttgatgc gtgtttttct ccataaatta tatatgtcca cattcggact taggggaaag
     4861 aataattgaa ccattcgtct gtaacgcagc ataatcgtta gcgcgaaaca taatatgttt
     4921 tctatgcatt gataattgat ggatcaactt attacgtccc tgaggaggga tgacaaatgc
     4981 actcctggaa aaagaaactt gtagtatcac aattagcatt ggcttgcact ctggctatca
     5041 cctctcaggc taatgcagcg accaacgata tttctggtca aacttacaat actttccatc
     5101 actaca
//
LOCUS       DAJTYH010000185         4960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTYH010000185 DAJTYH010000000
VERSION     DAJTYH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO609_003882"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO609_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6635100.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO609_003883"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO609_003883"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6635101.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO609_003884"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO609_003884"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6635102.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..>4960
                     /gene="clpB"
                     /locus_tag="OO609_003885"
     CDS             2745..>4960
                     /gene="clpB"
                     /locus_tag="OO609_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6635103.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMK"
BASE COUNT         1215 a         1205 c         1354 g         1186 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga
//
LOCUS       DAJTYH010000186         4918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTYH010000186 DAJTYH010000000
VERSION     DAJTYH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            49..123
                     /gene="tnaC"
                     /locus_tag="OO609_003886"
     CDS             49..123
                     /gene="tnaC"
                     /locus_tag="OO609_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6635104.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            344..1759
                     /gene="tnaA"
                     /locus_tag="OO609_003887"
     CDS             344..1759
                     /gene="tnaA"
                     /locus_tag="OO609_003887"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6635105.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1850..3097
                     /gene="tnaB"
                     /locus_tag="OO609_003888"
     CDS             1850..3097
                     /gene="tnaB"
                     /locus_tag="OO609_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6635106.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3229..4404
                     /gene="mdtL"
                     /locus_tag="OO609_003889"
     CDS             3229..4404
                     /gene="mdtL"
                     /locus_tag="OO609_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6635107.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4379..>4918
                     /gene="yidZ"
                     /locus_tag="OO609_003890"
     CDS             4379..>4918
                     /gene="yidZ"
                     /locus_tag="OO609_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6635108.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVP"
BASE COUNT         1117 a         1181 c         1230 g         1390 t
ORIGIN      
        1 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
       61 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
      121 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
      181 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
      241 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
      301 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
      361 tctccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
      421 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
      481 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
      541 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
      601 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
      661 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
      721 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
      781 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
      841 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
      901 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
      961 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
     1021 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
     1081 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
     1141 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
     1201 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
     1261 atatggcggc ttggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
     1321 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
     1381 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
     1441 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
     1501 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
     1561 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
     1621 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
     1681 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
     1741 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
     1801 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
     1861 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
     1921 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggggtgcctt
     1981 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
     2041 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
     2101 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
     2161 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
     2221 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
     2281 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
     2341 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
     2401 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
     2461 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
     2521 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
     2581 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
     2641 cggcaacatt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
     2701 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
     2761 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
     2821 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
     2881 ctttatttac gggatcggcg gtgccgggct gtgcgccacc atctgggcgg tcattattcc
     2941 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
     3001 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
     3061 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
     3121 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
     3181 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
     3241 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
     3301 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
     3361 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
     3421 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
     3481 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
     3541 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
     3601 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
     3661 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
     3721 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
     3781 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
     3841 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
     3901 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
     3961 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
     4021 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
     4081 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
     4141 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
     4201 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
     4261 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
     4321 agcatagtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
     4381 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
     4441 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
     4501 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
     4561 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
     4621 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
     4681 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
     4741 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
     4801 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
     4861 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccc
//
LOCUS       DAJTYH010000187         4822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTYH010000187 DAJTYH010000000
VERSION     DAJTYH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..1417)
                     /locus_tag="OO609_003891"
     CDS             complement(<1..1417)
                     /locus_tag="OO609_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6635109.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPE"
     gene            complement(1433..2317)
                     /locus_tag="OO609_003892"
     CDS             complement(1433..2317)
                     /locus_tag="OO609_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6635110.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2328..2933)
                     /locus_tag="OO609_003893"
     CDS             complement(2328..2933)
                     /locus_tag="OO609_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6635111.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3104..>4822)
                     /locus_tag="OO609_003894"
     CDS             complement(3104..>4822)
                     /locus_tag="OO609_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR6635112.1"
                     /translation="IINPDAYYWGLINDEDALKEIFKRSNIRMAGNVCNQMKKEALFR
                     PKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMRTLPRLKGISANLRKQLINSNDE
                     QTIESMARYMPDNEILELTDQQLGYQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNL
                     ALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQL
                     TYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDL
                     IRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNL
                     AKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETSADMILSELISHGEVDDQMLLNA
                     AALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEA
                     LRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALSNRHTPSAALAAEID
                     PEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAIREQAMR
                     KLDELY"
BASE COUNT         1400 a         1208 c         1003 g         1211 t
ORIGIN      
        1 cctccgggtt aaacgcatcg ccctgttcaa aacgcacccg cccagacatt ccccgttggg
       61 caatcatctc ttgcccctgt gcaacattta actcactgta atcacgtaac aaaatatcgc
      121 ttacggcagg ctcgtttgcc agcgcatcca gtacatagcg tccatgccct gcggcaatgt
      181 caaccacgcg gacggctaaa cctttggcgt ggagatcggc aacggcttgt ttaatcaata
      241 tttgcaaatg ggttttgcgc tggcgaatac cgcgccatcc aacactgttc aggtagattt
      301 tgtcgattaa acgcccgaat gcgttactgc cttgcggttg attttgatag acataatcca
      361 gggaactgcc tgaatcaaag ccggtactca ttccgagatg caggcccgca gagtgcgcac
      421 cgaacaattt catcgccttt cgcataaagc gatacgctaa atcaaccggc gataatggca
      481 cgggtccacc agaaaggagc cgccatcgat ccgctgatgg tcctgtgcgg tcttcatgct
      541 gataatcaaa tttttgcgat ttgttagcat ataagcggca gataaagctt tgcatttttt
      601 caaatgcctg cgccctgttc tcttcaccca atgtgtcgtg ataaaagcct ggcagcagat
      661 gcagctcttt cagagggcta cgtaatctct gataaaaatc aatctgcggt tggcgatgca
      721 ccacatagtc atcacctgat atcagaagtt gcgtggggag cgtaatcgcc gccgcatcgc
      781 taacaatacg ttcagacgtt ttatagagat cgagcaagat attgacggca atcgcccgtg
      841 tgatcagcgg atcattattg aaactcgcca cccgttgccg atcgtgggtc aaatagcgcc
      901 ctttcacata ggaattaata aaaaatagac cacgcagacg atgccataac gccagcgcag
      961 gacgtgccag cggcacatac aatttaacct taaaggccgg agaagccagc accagcccac
     1021 gaattgcagg tgcataatca tgaacccatg tggcaacaag cactgcgccg acgctttgcg
     1081 cgatcactac cacctcttcc agtccgacct ggctgtcgct ggcagcaaaa cggacaaatt
     1141 catcgacatc ctgcactgag cgcataagag acgggctata accacgcggc cccgaatttt
     1201 ttccatgccc ccgggcatcc caggcataaa aagcagtatc aggcatcgcc agttcatcaa
     1261 cgagatgttg tagacgacca gaatgttcat gcccgcgatg aaataagacg atgacctttt
     1321 tcgcccctgg ctgtaaagtg ggccagtgcc gataatacaa tgctgtatta tcactggtgg
     1381 taaaaaaatg ttccccaggg atgcgtgaat tttccatttt ctctattttc cttcagtaac
     1441 agcagtagcg aataaaataa aaaaataccg gagcggtgaa aattaatgag tcaatcctgt
     1501 caaggagtcc gccatgtcct agtaatagtt ttccactatc tttaacacca atatctcgtt
     1561 tgatggctga catcactaca tcgccgcaaa aaccactaat accaattaac aaacccgcta
     1621 ataatgcctg taatgtattt agcggtgtca gcaacggtcc aataatcagt gacgcaatca
     1681 tggtggtgat tacgcccccc agcagacctt ccagcgtttt tcccgggctg actttgggga
     1741 ccacttttct tctgccgcag gatttccccc ataaatactg tgcaatatcg tttgactcgg
     1801 ttaacgccaa aagaaataac accagtaacg ccccgccctg gatatttatg gttggcaaca
     1861 ttaatagcca ggcggcatga ctcaaggcaa aaacggtcat tatccatccc caaaaaatgg
     1921 cgctcacggt atgctgaaaa ccggaggggt cacccacaaa aactcgccag gtggctaata
     1981 tcagaaaccc agtcaaaggt ataaataaca gaaacagctc aaagcaatta aaaccgataa
     2041 gcaaatagtt aagaggaata acccaatata accaacgcga aaaatgtacg gatataagcg
     2101 tacaatattc tttcaatgcc agaaaactta tcagcgtaaa aaacgtcaac gtcatccagc
     2161 gtgggcctga aatcaccagt gaaaaacaaa tgacgatgcc ccaccaggta cgaatacgta
     2221 atgtaacttc atcccctttt ctctcaggta accgccatag cagaaaacgg tttatcagct
     2281 tgctaaaatt aacaacgcga aaagtgttgc cagagatttt tccagcatta tctttcagcg
     2341 ctcataagaa gcacactccg acaacgatta atcgcagtac agataagcag aattgaagca
     2401 atgctccata aaagattatt ccattgcatc cactgtggat aaatggcaac ggccagaccc
     2461 cacagaccaa atattaacgc gcgatcactt ttgccaaatg gaccggcata actgcgaaca
     2521 ccgttaatcg tctgcgcgag taaaccgcaa aactcggtca atatggtgca aaataacatg
     2581 agtatcacga gtgatgcgtt actttccggt aaaaataaaa acggtaaata gagcgcaata
     2641 tcggaaataa catcgccagt ctcattcaaa atcgccccca gacgtgtttg ctgattgcac
     2701 tcacgcgcca acatgccatc cagcgcattg agcgccatac ggataaaaag cacgatgggc
     2761 aatagcaaaa agaggatggg ttgtgccacc aacaccaaca gcaatccggt aaaaagagaa
     2821 agcgccagtg ccgtaagagt gatgtgattc gctgtaacgt ggtgcttata aagccaaaac
     2881 atcgtcggcc ttaacagcga ctgaaagagc ggttttatct ggtagagcgt cattatcaat
     2941 ccttgataat tcggtttgcg ctatggtgca tcaattcccc tgacggagat atcgcaacac
     3001 gccgcttcat taaaaattca gaatatatca aggtattggg ggctcactgt caggaatgat
     3061 tatccccaat aaatcaagta gttaaaaatt attacgctgt tattcaatac aactcatcaa
     3121 gtttcctcat cgcttgttcc cgtattgcgc gcgtcttacc gttaagcgcc agctggcgga
     3181 ctaaatccag ttcagggtta acaagctcta acgccagcaa aggatcacgt ttcagcctcg
     3241 ccttcaatac atcaactgga aagcggggat tattcatcgc cacaatccac cactcgggat
     3301 caatttctgc cgccagtgcc gcagaggggg tgtggcggtt gctaagtgcg ccgaacttga
     3361 aaaatgtgag attgcttttc gccagtgtac tccaccatat acgatccggt tgttttgcca
     3421 gtacatccag ttgtgctaac gcatcggggt cgagtgcata ataaacacgt aatgcttcat
     3481 caacctgcgc acgggagcta ttttcctgcc tcagaaaaaa cttcgcccaa atatcattgc
     3541 gtggcgtgtt ttgctgtaat tccttgagaa caacagcggg taaattatcc caactaacta
     3601 atgcactctc taaaaagtcc cagtcttcca aacgtatcag ggcggcggca tttaatagca
     3661 tctggtcatc aacttcgccg tggctaatca gctcagacaa aatcatatca gcgctggttt
     3721 catatgcatc tgaatctgct ttcggattat ttttccgatg cgataaagca cgttgccaaa
     3781 ttttttgttt catcgacgtg ctcagatact gatttttcgc cagattactc cacccctcag
     3841 gatcgttgaa acgtgtcaat aaccgttgta aagcagcgtc agagtgaatt acctgcatat
     3901 cccgacagca aaagtaggca ggggattttt tctccatcct tttaacaaaa ttaaactgta
     3961 agttatctgg caatgctaaa aataacacac gtattagatc atcgtcattc tcgaattttt
     4021 ggtataaata ggctgcaact ttgtcactct cttgtggcgt catccgcaat aattgaccat
     4081 ggtgtttcat ctctgccagc ttgaacgcta agcttgtgac aacattgttt gatgaatcgt
     4141 tcgccaactg cattgcctga ttgtaagtga gttgtcctcc agcaccgacc tgacgaatag
     4201 aggaatcatc actgcggata tagccatcaa taatggattc gggcaataac cacgcatctt
     4261 tataaagctc ctgaatgatc atatcccaat ctcttttcgc cagagtcatg acgatatcca
     4321 gagggagatt ttcacgtagc gcaagattca acggaccgac accatcaggt agacgactca
     4381 ttcgcgtcat tatttcgaca gagagcggtt cacggtcgag caaccccaga acaacaggct
     4441 ggtaacccaa ttgctgatcg gtcaactcca gaatctcatt gtcaggcata tatctcgcca
     4501 tactttcaat cgtttgttca tcattgctat taataagttg tttgcgtagg ttagctgaaa
     4561 ttcctttaag ccttgggaga gttcgcatta tggcaagatc aaatgtcgca atatctcgtc
     4621 cttcaaatgc agcaacgttg ccttcatcca gcatttgcaa ttcctgcact aactcaggag
     4681 aaggcttagg gcgaaacagc gcttcttttt tcatctgatt acagacattc cctgccatgc
     4741 gaatattgct tcgcttaaaa atttctttca gagcatcttc atcatttatt agaccccaat
     4801 aatatgcatc aggatttatt at
//
LOCUS       DAJTYH010000188         4818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTYH010000188 DAJTYH010000000
VERSION     DAJTYH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..1076)
                     /locus_tag="OO609_003895"
     CDS             complement(<1..1076)
                     /locus_tag="OO609_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR6635113.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALV"
     gene            complement(1288..1635)
                     /gene="pspE"
                     /locus_tag="OO609_003896"
     CDS             complement(1288..1635)
                     /gene="pspE"
                     /locus_tag="OO609_003896"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6635114.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1678..1899)
                     /gene="pspD"
                     /locus_tag="OO609_003897"
     CDS             complement(1678..1899)
                     /gene="pspD"
                     /locus_tag="OO609_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6635115.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1908..2267)
                     /gene="pspC"
                     /locus_tag="OO609_003898"
     CDS             complement(1908..2267)
                     /gene="pspC"
                     /locus_tag="OO609_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6635116.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2267..2491)
                     /gene="pspB"
                     /locus_tag="OO609_003899"
     CDS             complement(2267..2491)
                     /gene="pspB"
                     /locus_tag="OO609_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6635117.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2545..3213)
                     /gene="pspA"
                     /locus_tag="OO609_003900"
     CDS             complement(2545..3213)
                     /gene="pspA"
                     /locus_tag="OO609_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6635118.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3380..4357
                     /gene="pspF"
                     /locus_tag="OO609_003901"
     CDS             3380..4357
                     /gene="pspF"
                     /locus_tag="OO609_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6635119.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4477..>4818)
                     /locus_tag="OO609_003902"
     CDS             complement(4477..>4818)
                     /locus_tag="OO609_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6635120.1"
                     /translation="AVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGS
                     MIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDA
                     AMKILQDALSD"
BASE COUNT         1197 a         1212 c         1145 g         1264 t
ORIGIN      
        1 tcgaccagcg ctaatatttc gctttctgga agcaagccac gcagtgggtt caacccgatg
       61 ccatcgtgag aggcgaggaa gttaaaccag gtcgttttac tggagggcaa gctcagactt
      121 tgcgcccact ggcacagcgc ctcgacgttc tgtttttgca ccgcatgtaa caccagcggc
      181 ggcagcgaga actggtacac catatgcgct tcgtcatcac cgttgccaaa gtaagcaatg
      241 ttgtctttgt gcggaacatt ggtttcggta ataatcactg tacctggtgc tacgtcatca
      301 ataatcgacc gtaacagctt gataatcaga tgtgtttttt ccagatggat gcagcttgtt
      361 cccggctctt tccacataaa gccaacggca tccaggcgga catactcagc acctttctca
      421 aggtaacaga gtaaaacatc caccatcgcc agcaacactt cagggctacg gtaattcagg
      481 tcaatttgat cgtcactaaa ggtggtccat aaatggcgcg tggaattatc atccatttga
      541 aacggcgtta atagcggtaa cgcacgcgga agggtgacgg cactgagatc ggttttcgga
      601 tcaaccgcaa taaagaaatc ttcaaaaccc ggaagctgtt gtaaatagtt tttaaaccat
      661 tcactttttg cagacatatg gttgcagaca aaatcaaaca ttaaatgact gcattcaccg
      721 agttgctgaa tatcctgcca ctcccccgct tcactggcga cctgatgata atcaattacc
      781 gaaaatccat catcagatga ccatgggtaa aaaggtaata aatgaacatg tgaaaaaatg
      841 ctttgcagcc attgatggta gaactgatta aatatgggta gtggttttaa atcattactg
      901 tgaaattgat cggcataggt aattaacacg acatcacttt catcccagtg ttttttacgt
      961 cgctgtgtaa ttaatcgttt cgcactctca agacgcgtta caagtttctg taaatgagtt
     1021 gcggtaaatg ttccaccgta gatttcgtcc atgtaatccg taattttctg tttcatgttg
     1081 cctttccgtg gtagcggtcc catgaggcga gagactactc cgcagaaaat aaactgtcaa
     1141 ttgagcacaa gggttttagt cacgcaggaa atcatttttt accagggaaa aagcgtattt
     1201 gtgagctgac gcaaataatc tatagggatg taaataaggt gtgacagaaa aaaaacggcg
     1261 cataagcgcc gctcatggtg aattctttta acctttgacc ttcggcattg cgatgtcttt
     1321 caggccaccg gcgttctcaa cgtgggtata tcccatctcg ctaaggagct cttttgcctg
     1381 cccggactgg cgtccggcat tgcaatacac tttcacggtg tcgtttttat ccggaacggc
     1441 ggtggcaatg cgctctttca cttccttcag gggaatattg atggccccct gaacgtgctc
     1501 ttgctgatac tgctctggaa cacgaacatc gatccagtgt tcagcggcga aaacgggcag
     1561 tgaaaacacc agtgccagag caagtaagcc tttttttaaa catgtaagcg tccttctatg
     1621 gatgaaaatt gccatccatt atacgcctga gatttaacaa tttttgcgaa actcccctca
     1681 ccttttataa cgctgtgcca gtttattagc agcccgactt aacagcggtt ccagcgccac
     1741 agccagcaac attttcagcg gtcggcgagc aactgatttt atcgcccagc ccgccacacc
     1801 cgccgggcca tagcgcagtg cggtaagaag taccagcttg cctgctaatt tgaaaccaag
     1861 cttcaccttt tgcccggcct gttgccagcg agtattcata actttcctca cagttgacgg
     1921 aaacggctac gtaacgtgaa agtatcggaa gtgacataac gttccatctc gcgtaaacgc
     1981 gtttcacttg ccgccagttc acggtcgact tcatccagca attcgctgct ggaaggtagc
     2041 tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa
     2101 gcaaccaggg taaacagcgc cagaccgaaa aaaatcgaca gcaccaccag gatacgcacc
     2161 agttttaccg gtacatcaaa atagttggca atcccggcgc agacgccgcg gaccatgccc
     2221 tgctgtggaa tacgccataa ctttttattg agattaatgc ccgccattag cgatccctcc
     2281 agttcggatg ttctgcatca agaatagatt ccagcgcctg aatacgttcg cgcatccgtt
     2341 ttgcttcatc aaccagttgc gctaatcgct gctgctcact ttgcgacaat tcactgcgac
     2401 cagaacgatt gctgtaatgc agccataacc agatcggtaa aacaaacagc acaaaaatgg
     2461 ttaacggaat agccagaaat agcgcgctca taagtactcc ttagacgatg agcggcggcg
     2521 cgttcggacg ccgcctggat gttattattg attgtcttgc ttcattttgg cttttaattg
     2581 tgccagttgt tcgctgattg catcatcggc tttcagttcg gcaaactgat cgtccagcga
     2641 tttttgttta ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa tacgacgttc
     2701 gaaagattcg aaacgagcca ttgcttcatc cagtttgcca ctgtccagct gacgacgcac
     2761 atcgcgcgac gagtttgccg cctgatgacg taacatcaat gcctgctggc gagcgcgtgt
     2821 ttcgctcaat ttgttttcca gctcgccaat ctctttcttc atgcgtgcca gcgtatcgtc
     2881 caccagcgtc acttcatgtt ccagggactt aatcagatcg gtcagtttct gtttttcaat
     2941 taacgctgca cgtgccagat cctctctctc tttcagcagc gccagttcgg ctttttcctg
     3001 ccattcaact tcacgcgccg acgcttgttc aatacggcga gtcagctgtt tcttttctgc
     3061 caacgcacgc gccgaagtag aacgtacttc aaccagtgta tcttccatct cctggatcat
     3121 cagacgcacc agtttctgtg gatcttccgc tttctctaac agagcgttga tgttggcatt
     3181 cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg tcctcttgat ttctgcgttc
     3241 aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca attttttatc tttttgattt
     3301 ataaagatct gattgaagaa tcaacagcaa catgccagga tgaattagcg aattacacta
     3361 acaagtggcg aatttcatca tggccgaata caaagataat ttacttggtg aggcgaacag
     3421 ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg ctggacaaac cggtgctcat
     3481 catcggcgaa cgcggcaccg gtaaagagtt gattgccagc cgcctgcatt atctctcctc
     3541 ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg ttaaatgaaa atctgctgga
     3601 ttccgaactg tttggtcacg aagcgggggc gtttaccggt gcgcaaaaac gtcatcctgg
     3661 gagatttgaa cgtgccgacg gcggtacgct atttcttgat gaactcgcta cggcaccgat
     3721 gatggtgcag gaaaaattat tgcgtgtgat tgagtacggt gaactggagc gcgttggcgg
     3781 tagccagccg ttgcaggtga atgtgcggtt ggtatgcgcg acgaatgccg atctcccggc
     3841 gatggtcaat gaaggtactt ttcgcgctga cctgctcgac cggctggctt tcgatgtagt
     3901 gcaactgccg cccctgcgcg agcgcgaaag cgacattatg ctgatggcag aacactttgc
     3961 cattcagatg tgtcgggaaa tcaagctgcc tctgttcccg gggtttacgg agcgcgccag
     4021 agaaacattg ctgaattatc gttggccggg aaatattcgt gaattgaaaa acgtggtgga
     4081 acgttcagtg tatcgccacg gcaccagcga ttatccgctt gatgacatca ttattgatcc
     4141 ctttaaacgg cgtccacctg aagaagccat cgccgtttca gaaaatacct cgcttccgac
     4201 gctgccgctg gatttacgcg agtttcagat gcagcaggaa aaagagttgc tgcaactcag
     4261 tttgcaacag gggaaatata accagaagcg cgcggctgaa ttactggggt taacttatca
     4321 tcagttccgc gcgttgttga aaaagcacca gatttagcga agttgagcaa tacaacttgc
     4381 cggatgcggc gtgaatgctt tattcggcat acataatcaa tacgtcgttt gcaggcctga
     4441 taagcgcagc gcatcagaca ttattgcgtt gggctattaa tcgctcagcg catcctgcaa
     4501 aattttcatt gccgcatcga attgcgcatc cgggatggtc agcggataca ggaagcgaat
     4561 cacgttgccg tatgcgccac aggtcagcag gagcagcccc tgcgccagcg cgcgttgctg
     4621 gattttctgt gcaatcgccg ctgacggctc gcccgtttgc ggatcgttaa actctgccgc
     4681 aatcatcgac cccaggccgc gtaccgcagc aatggccgga acgctttctt tggcatcaat
     4741 caacgtgttt ttgagacgct ggcccagttg attcgcgcgt tcgcagagtg attctttgtc
     4801 gataatattg agcaccgc
//
LOCUS       DAJTYH010000189         4804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTYH010000189 DAJTYH010000000
VERSION     DAJTYH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..279)
                     /locus_tag="OO609_003903"
     CDS             complement(1..279)
                     /locus_tag="OO609_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635121.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO609_003904"
     CDS             220..405
                     /locus_tag="OO609_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635122.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO609_003905"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO609_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6635123.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO609_003906"
     CDS             complement(1254..1670)
                     /locus_tag="OO609_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635124.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO609_003907"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO609_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO609_003908"
     CDS             1837..2862
                     /locus_tag="OO609_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6635125.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO609_003909"
     tRNA            complement(2915..3004)
                     /locus_tag="OO609_003909"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO609_003910"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO609_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6635126.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO609_003911"
     tRNA            3996..4071
                     /locus_tag="OO609_003911"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..4804
                     /locus_tag="OO609_003912"
                     /pseudo
     CDS             4233..4804
                     /locus_tag="OO609_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         1301 a         1121 c         1096 g         1286 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcgg
//
LOCUS       DAJTYH010000190         4750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTYH010000190 DAJTYH010000000
VERSION     DAJTYH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            130..387
                     /locus_tag="OO609_003913"
                     /pseudo
     CDS             130..387
                     /locus_tag="OO609_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(437..1387)
                     /gene="uspE"
                     /locus_tag="OO609_003914"
     CDS             complement(437..1387)
                     /gene="uspE"
                     /locus_tag="OO609_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6635127.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1539..2291)
                     /gene="fnr"
                     /locus_tag="OO609_003915"
     CDS             complement(1539..2291)
                     /gene="fnr"
                     /locus_tag="OO609_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6635128.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2486..3001)
                     /gene="ogt"
                     /locus_tag="OO609_003916"
     CDS             complement(2486..3001)
                     /gene="ogt"
                     /locus_tag="OO609_003916"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6635129.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3012..3155)
                     /locus_tag="OO609_003917"
                     /pseudo
     CDS             complement(3012..3155)
                     /locus_tag="OO609_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3545..4360)
                     /locus_tag="OO609_003918"
     CDS             complement(3545..4360)
                     /locus_tag="OO609_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6635130.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTCYSPGKFTRRLTMHNYAITRQMI"
     gene            4457..>4750
                     /locus_tag="OO609_003919"
     CDS             4457..>4750
                     /locus_tag="OO609_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HCR6635131.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKA"
BASE COUNT         1242 a         1085 c         1128 g         1295 t
ORIGIN      
        1 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
       61 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      121 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      181 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      241 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      301 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      361 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      421 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      481 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      541 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      601 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      661 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      721 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      781 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      841 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      901 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
      961 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1021 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1081 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1141 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1201 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1261 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1321 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1381 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1441 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1501 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1561 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1621 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1681 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1741 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1801 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1861 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1921 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     1981 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2041 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2101 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2161 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2221 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2281 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2341 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2401 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2461 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2521 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2581 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2641 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2701 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2761 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2821 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2881 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     2941 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3001 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3061 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3121 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3181 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3241 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3301 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3361 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3421 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3481 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3541 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3601 taccgggcga gtagcacgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3661 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3721 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3781 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3841 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3901 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     3961 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4021 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4081 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4141 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4201 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4261 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4321 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4381 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4441 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4501 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4561 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4621 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4681 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4741 tagcaaagcc
//
LOCUS       DAJTYH010000191         4695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTYH010000191 DAJTYH010000000
VERSION     DAJTYH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(241..486)
                     /locus_tag="OO609_003920"
     CDS             complement(241..486)
                     /locus_tag="OO609_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635132.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1263..2105)
                     /locus_tag="OO609_003921"
     CDS             complement(1263..2105)
                     /locus_tag="OO609_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6635133.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2190..2387)
                     /locus_tag="OO609_003922"
     CDS             complement(2190..2387)
                     /locus_tag="OO609_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635134.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2407..2895)
                     /locus_tag="OO609_003923"
     CDS             complement(2407..2895)
                     /locus_tag="OO609_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635135.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2892..3269)
                     /locus_tag="OO609_003924"
     CDS             complement(2892..3269)
                     /locus_tag="OO609_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6635136.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3316..3693)
                     /gene="cbtA"
                     /locus_tag="OO609_003925"
     CDS             complement(3316..3693)
                     /gene="cbtA"
                     /locus_tag="OO609_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR6635137.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3773..3994)
                     /locus_tag="OO609_003926"
     CDS             complement(3773..3994)
                     /locus_tag="OO609_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6635138.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4081..4557)
                     /locus_tag="OO609_003927"
     CDS             complement(4081..4557)
                     /locus_tag="OO609_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6635139.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4573..>4695)
                     /locus_tag="OO609_003928"
     CDS             complement(4573..>4695)
                     /locus_tag="OO609_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6635140.1"
                     /translation="MTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1094 a         1128 c         1240 g         1233 t
ORIGIN      
        1 aagtatgtca ggcttttttc cgtaatgctc taacttccac gcattggttt ctgacatcaa
       61 ataaggctat gactttctct cccttttaat ctcattctga tctaaaacca tatcttaaaa
      121 aaatgcatgg gtattttaat tattttgatg ttgatctaac gcactcaagg accattttat
      181 taccgattat ccattctatt taactgttgc gatataaatg tttgttttag gtgctttaat
      241 tcagtacaag tcatcaaacc gtaccagacc ctgactgtca gtgacaccga caggtggggg
      301 cggcggtgtg gggtcaaact gagtgtgtct gcctctggcc cacagagcca gctctgccgc
      361 cattgcccgt ggatgatact ccagctgcac gttgagaccg gaaactgggt tgttgttctc
      421 atccacgaac tgcagcgcca gccagtgcat ccgctgctta ccgcaggcga cttctttgta
      481 cttcattttg tgactcaggc aggtgactgt cgtgttgcct gttgcgtcca ttttgcggtt
      541 aaaccctttt aattcaattt gttaaattgc tttccacctt ttgcggggcg cgacttaacc
      601 aaaatattca ctgttatcat aatatgcaat gggctggagg ttgcaggtca tttagggatt
      661 ttctgtaagc cccgttttgc agaacgttct atctccgatt gagatcctgt ctctcggtat
      721 taccggtagt ctggccataa tcccctatgg atcatattga tatagtaggc ataacaccca
      781 aaagttgact aaagctaaat caatcagagt gaaaaatgtt cggtttgatg ttggggggga
      841 ctgaggaggg gccaacgcag gtggtatgcg ttttacttca gaccggacaa agaccggatg
      901 tatccgtgca gtaatgaatc cgttagccag gtgctcatgt ggaaatagtg tctattgggg
      961 atttgaagtt tgacagaaaa ggccacagtg accagaaggg ggctacttag aagaaatgtt
     1021 caggctgggt ggaagaggta aagcagtgac atcagcattc aaagtgaccg gatatctacc
     1081 agaaccccag aaagcaaaaa accagcccgt aggctggttc ttctaaatag tggtgccgga
     1141 ctcggaatcg aaaatttctt ttatcatatt gatattaatg caatttgttt ggtttgatta
     1201 aaaatgtacc ctcaattata cccgcagtcc cttttgatat cgaaagggaa tcactctcgg
     1261 gatcaggctg agagcacaga aggaaagtac ctggctatta tatcgttaat tctgtcaatc
     1321 agttccagcc ttttgaaagt gatgtgtgca gatcccttct taaagtattt aatgatgaac
     1381 atctcgtctt catagcgctc tttaccctga acggactgga tgtgaccatc cagacgaata
     1441 gtgatgtttt cccggttgtc ggggatcggt ttgccgctga acagatgtag cattctttcc
     1501 aggtcgacga gtcggtcagt ctgttgcccg gtgataagat gaaaccccca ccggtcccac
     1561 ctcaccaggt tgttgacgat aattttactg ccaaatttac agggtgagtt ggttttgtaa
     1621 tcccagctca gccctttgaa gacgttgatc actccccgtt caaacacttc atctttgttc
     1681 tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatca
     1741 tattccagtg acctgtacca tgtgtcccgg gtctgcgcat ccatcagact cagcatcccg
     1801 gaacgttgca tcagatcacg ccagagttcg cgatcaagat tgcgtgtgat ggctttcatt
     1861 gcggtttccg gtttctccat cagccagcaa ccgcagcgaa aatcctgttt catggcccag
     1921 tccagagccg ttttacctct aatactttct gtcattgctg atacttcagc cagttgtcgg
     1981 ataagtgtgt cgatttgcag caatgtactc tttcgtacag ccagaatgtg gcggatactg
     2041 gttgagcaga taacgtccgt atgcccgttg accacttccg gacgagaggc tgtgatttca
     2101 gacataatcg atattccttt ttgcagataa acgaaaacgc cagccgtatt caggctggcg
     2161 tcagtggaat gaagtctggt gagtaagact caccggttct gttgcagaaa ctgcagctga
     2221 cgcagggtac ggatatcttc cctggcctgt tcaatacagg gcagcagtgc tgccgaatcc
     2281 gttttatcct catcgttgtc gaagataatc cctgattcgc agtcaatatt gtcctgcagc
     2341 cacgcaatca gaatatccag cgccgtttct gtggttaata atttcatcca gtgtcttttg
     2401 tcctgattac cagtcgaaag tggggaaccc cggcagacat tctgcgccgg cgtccagatg
     2461 aatgagactg acgccataac gccgggtgag agtgacgacc agacgacgga acgttctgga
     2521 caggcccagg cgtttaagcc gcagaaccgg gtacgaccat gcatcggtac gtaacagata
     2581 gccgctgcct gtaaaatgaa tccattctgt ttcgccgtcg tcattagtct ggtgtgacag
     2641 cgagtacagc caggcgttgt ccgtttccgt gatatgtgcg gtgctgcaac gtatgcaggt
     2701 aagggttgtt aacggtggtg gtgtactggt tgcgtgttgg tcggatttat cgaccacgcg
     2761 gagtgaataa ccgttttcag agaccttgtt aatcagttca ctgagattaa caccatcgac
     2821 gtcaacgaca atgcgcccca tgttcagtgc ctgcacgtta acgctgtcgg cttccagcgt
     2881 cagggccagt ttcattgttt cgcttccgga tgcttccccc gggtaatgtt atttaccgtt
     2941 ctgtagttat cgcgggtcat caggccggtt gcccggcgtg cccggagaat atcaatgctg
     3001 ttgattaact gcgacggggc tcctgcgctg aatcccggct ggtcggtacg taccagcgcg
     3061 tatttttcaa cgagaaagtt caccgcgtca cacagtgaaa ttcctgcctc aatatgctgc
     3121 tcaatcacac gttcatcagc gaacggtgtg tcattcagcg tgaggccata gtgctggtcc
     3181 agcagtcggg tgagcagtgt ctgccagatg gtgatgggag acgggcagcc cgatgcctcc
     3241 cgtacgtgag tgtcgggtaa tgtattcatc ttaagttctc tgtaacaggt ggttataaag
     3301 gggagatatc cggtgttata cggtggttgc gggcgctgcc ggtgtcggat aaacagccag
     3361 ataaacgtaa ccacaggagc cgagggtgtc ggcttcgcag gtcagccctt tcgcatacag
     3421 tgtgacggta tgctgatggc ggggattcag ttcaccgcca gtgagcatga gttccagttg
     3481 tttcatcagc agcggaaagg cctggtccag gtggtacgca tctacgtcgc tgaacctgcc
     3541 tctgataccg gcgcggtctg caaggtaatg caggcggtta ccctcctgta ccagtcgggc
     3601 cccgaaacag ggcgtcacgg tgcagggcag cccccaccag gggcggtcgt gattgtcgtc
     3661 gggaggcgtt gtcccgggga gtgtgtctga cacgataaag tcctcacaga aaatcggtta
     3721 agaatggtcc ggtagtggcg ataattctgc tcatcagaat tcccttccag actcagttca
     3781 gggtgacgct catcagccgg acatacggac caaaactgtc cttacggcgt tctgcaaaca
     3841 cagccagtac accgggaata tcctgcactt cacggccggt atatgtatcc gtgctaccgt
     3901 gccagcggta tttaccggta cagaacggaa aaagacggga tgcaggatgc tgacggtgaa
     3961 tacgcatggc ttcaccacgg gtgatgattt tcatgagaat agtcctcctg aatgtgaaag
     4021 taagaaacag ctgagaaaga gaaactgaag tgatatgaag gtgattgtga cggtgacggg
     4081 ttaaagcaga ccgtgttccg caaaggagaa aacctggctg ccaccgacta tcagatggtc
     4141 cggtacccgg atatcaacca gggccagtgc ctgtaccaga cgctcagtga taaggcggtc
     4201 tgccttgctg ggggggactt caccggacgg gtgattgtgc gccagcacca cggcagcggc
     4261 attgtggtac agggcgcgtt taatcacttc ccggggatgg acttccgtac ggttgatggt
     4321 gccggtgaag agggtttcac cggcaatcag ctgattctgg ttgttcagat acagcacccg
     4381 gaactcttca cgctccagtc ccgccatgtt cagaatcagc cattcccgtg ccgcatgcgt
     4441 ggaggtgaag gccacgccgg gttcatgaag atggcggtcc agggttttca gggcccgcag
     4501 aatgaggctg cgctctctgg ttgtcatttc tccgggcaga aaggaaagct gctgcattgt
     4561 ccctgccacc attcagtcga tgatgcgcat aatggcgctg cattccggat gctgcagggc
     4621 gtaatcccgc agccggtaat aatggaccgt catggcataa cactccgtac gacaggcatg
     4681 atgactgtac gtcat
//
LOCUS       DAJTYH010000192         4666 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTYH010000192 DAJTYH010000000
VERSION     DAJTYH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..97
                     /locus_tag="OO609_003929"
     CDS             <1..97
                     /locus_tag="OO609_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6635141.1"
                     /translation="FHWGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(269..598)
                     /locus_tag="OO609_003930"
     CDS             complement(269..598)
                     /locus_tag="OO609_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6635142.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(603..788)
                     /locus_tag="OO609_003931"
     CDS             complement(603..788)
                     /locus_tag="OO609_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6635143.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(785..3424)
                     /locus_tag="OO609_003932"
     CDS             complement(785..3424)
                     /locus_tag="OO609_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6635144.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3632..4621
                     /gene="ldhA"
                     /locus_tag="OO609_003933"
     CDS             3632..4621
                     /gene="ldhA"
                     /locus_tag="OO609_003933"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6635145.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT         1164 a         1217 c         1080 g         1205 t
ORIGIN      
        1 ctttcattgg ggattttctg accagagtca tttttcaacg gtatttaagc aacgctttgg
       61 gatgacgcca ggcgagtatc gacgtaaatt ccgctaacta attggcgttg catgtactgc
      121 aacgccaatt agttaaaaca gatatttgac tatgcatttt tcagggcgaa aggcaatttg
      181 acaggagttt ttgaatattc accttgtttc aggaaaatag atcaatcgct ttctcctgta
      241 gcgttatccc gatattgcgg ttttgctatt aaaactttcg cacccattta ccgtttatcc
      301 cttgtacata ttcccccggt ttggcccgag ctacgagctt ttgcccggcg agtttcgcga
      361 tatcatctac agacacatta ttttgctttg cccgttgttg gtaactcgct ttacgttcgg
      421 cattaatttc ggttactaat ttctctgttt cagcatccgt tttcagcgca accagataac
      481 cataaaatgt ttcacctacc cgcccctgag ttctggcttc atccagagtc aatgccatta
      541 cattgctgct taccagccca acaagaaacg cacaaagaag taatgttttc ttcatcatcg
      601 cctcagaaaa gatcgctacg agtttcaagc agctcttcga catctttgtc tgccttgatg
      661 atgatctcat gctcaatttt aacgttcata ttgatagtga tcggttcctt aggtgcagcg
      721 acttcaatgc gaggcgtaca gccaaccagc ataaaagatg acgtcaacgc agccagtaaa
      781 attttcattg tttttcctca cactcttttc cttgcggaca gtcatttccc ggcagacgtg
      841 cgttctgctc caaccatgcc tggagattat cgccaaagcg taaactgcgc cacagcgtaa
      901 aaatattctc ttcatgatgg taattaaggt ttaccgtacc gcttttacca tcaacgcgac
      961 tggtacctgt aatgttggcc tgcatggtta ataaaccgag attatctaag ttaatttttg
     1021 tcgatgaccg gctaatttcc atatagcgca accagttaat tgctgaaccc gcagtcatat
     1081 tgtctttcac caccgcatct gcggtatctt tatccagccg caaggtcatt agcccgctat
     1141 ttgccagcca accatctttc actatccatt tttcgttgtt tagccataac ggcaatgcac
     1201 cactaaatgc cccggacatg gcgaattgtt tcggattgac ggcgctaacc agttcgctgg
     1261 atgagagatt attcagccgc aacagcgccg ggtcatgttg cggcatacgt aattgtttca
     1321 tcagcacgtt accgcctaac acatcgacgc taacatcagt caacagcaag ggttcttctt
     1381 cagtccacgg ataacgccct tgcaaatcag ccgtaatatt tttcgctgtc accagattaa
     1441 tcacttcggc aattcgcaac gtaacggggc cgcgagtccc cagatgccag gctccatcgg
     1501 caaaacggaa aggcaggaca aaatcgacac cgttaacctg attatctggc atccaggcac
     1561 taccgccttt caacacgccg tgccctcccg cgcggaatcc ttgttcaggc gcagcagaaa
     1621 atgcaacctg agcatacagt tcaccatcgc gtaagttcat cttccagtcg ggtggcacca
     1681 gcggctggaa tacagtcagt gattgttttg gccaccaggc gttaccgcgc agacgaatac
     1741 cgtcccagcg accatttacc cgaaccgggc caatctcacc agcatgtaaa tcgcctttaa
     1801 agaggaaata ggtcggattg cgcccatcga cgctaaattt taaggttgat ggcggtaaca
     1861 cactgccgcc agtgaacagc gtttgcccgg cgtccagtga cagcgcgccg ctgaaggagg
     1921 gatgctgtgc gtcacgtacc cagacgacgg gcttgtcgag aattaatcgc ggcttttcta
     1981 ccgtcatcgt accgtattgt aactggtcga aaccggtgga gagatcggtc agcgtaatcg
     2041 tgctgtcatg ccactcacct tttcctgcga catcccaggt ggcattcatc ggtgtaaaac
     2101 tcccttttcc ccagtagcgc cactgccagc ggccagcgtc agggagaaaa tcattcgcca
     2161 gcccatccat atgcagcacg aaatcgccca gttcattttc atgcgcctgc aaaatggcct
     2221 gcaaacgtcc gtcaacacca cgttgggtga cttttacacc cgctaaaggc cagcggattt
     2281 catcgatgtc cagcgaatcg atgactcttc cctttgaacg aagtaacgcg cctggctcaa
     2341 aggtcagcgt tgggtcagac agacttccac ttagctgcgc aggtaaacgg gcatataaaa
     2401 tgaggtccgc ctgtttcgct tcaccggtca gctgcagagg cagttgactg ttatccatgc
     2461 ttaattttcc tgggccaaaa ttaagcaccg cgttgccctt acccgcttgc ccctgggtca
     2521 gcacattcag tcgtccgctg accagagcgt tctcaagccc tgcctgccag ttgtcgactt
     2581 tgacacccag tcggccactc aaaggaaaac cagcatacag ccagctccag cgaccatcgc
     2641 ttacggtcaa ttgttgacga gtaatttgcc acggcaaatc gagcaacgga tcgccgttat
     2701 cccgtgccag cacgatcaat tgcccgctat tttcctgcca gtccagctcg gcatccacca
     2761 gtgacggttc ctgcggcaag tttaacgtcg cagtagcatg cccacttaca ggaagtccat
     2821 ccggcacgag cggcatagta aattccccca ccagtttaac cggcggctga ttttcaaacg
     2881 cgacgacatc cagttcgctg actgtaagtt gttgcccttt cagctggcct tgaaatttaa
     2941 ctttttcgcc ctgataacgc agttgctgga tatcagaggt taatgcgaga gagagttttc
     3001 cctgccattc ctgccaggga gaaaaaatca gtttatcgat attgatccag gtgttaggca
     3061 gcatggactg ccactgcgcg agggtttttg gagcagctgg cgattgctcc gtctgcggca
     3121 atttcgccag acaagcagaa tcaagttcta ccgtgccgac gttcaataac cagcggctgg
     3181 gatgtgaaag gctggcgttg gtgatatgcg caagctgaca atctcccacc agataacgga
     3241 gatcgggaat gattaaacct ttacgcgtaa tgcgtgggct ttcatctaat gcaatacgtg
     3301 tcccgagcgg tagccagatg tccgccagcg tgggaaccca cagcccgagc gtcatcagca
     3361 gcgtcaacgg cacaagaata atcagtaata acagcgcgag aacggcttta tatttaccca
     3421 gcatgggtag ttaatatcct gatttagcga aaaattaagc attcaatacg ggtattgtgg
     3481 catgtttaac cgttcagttg aaggttgcgc ctacactaag catagttgtt gatgaatttt
     3541 tcaatatcgc catggctttc aattatattt gaaattttgt aaaatatttt tagtagctta
     3601 aatgtgattc aacatcactg gagaaagtct tatgaaactc gccgtttata gcacaaaaca
     3661 gtacgacaag aagtacctgc aacaggtgaa cgagtccttt ggctttgagc tggaattttt
     3721 tgactttctg ctgacggaaa aaaccgctaa aactgccaat ggctgcgaag cggtatgtat
     3781 tttcgtaaac gatgacggca gccacccggt gctggaagag ctgaaaaagc acggcgttaa
     3841 atatatcgcc ctgcgctgtg ccggtttcaa taacgtcgac cttgatgcgg caaaagaact
     3901 ggggctgaaa gtagtccgtg ttccagccta tgatccagag gccgttgctg aacacgccat
     3961 cggtatgatg atgacgctga accgccgtat tcaccgcgcg tatcagcgta cccgtgacgc
     4021 taacttctct ctggaaggtc tgaccggctt tactatgtat ggcaaaacgg caggcgttat
     4081 cggtaccggt aaaatcggtg tggcgatgct gcgcattctg aaaggttttg gtatgcgtct
     4141 gctggcgttc gatccgtatc caagtgcagc ggcgctggaa ctcggtgtgg agtatgtcga
     4201 tctgccaacc ctgttctctg aatcagacgt tatctctctg cactgcccgc tgacaccgga
     4261 aaactaccat ctgttgaacg aagccgcctt cgatcagatg aaaaatggcg tgatgatcgt
     4321 caataccagt cgcggtgcat tgattgattc tcaggcggca attgaagcgc tgaaaaatca
     4381 gaaaattggt tcgttgggta tggacgtcta tgagaacgaa cgcgatctgt tctttgaaga
     4441 taaatccaac gacgtgatcc aggatgacgt attccgtcgc ctgtctgcct gccacaacgt
     4501 gctgtttacc gggcaccagg cattcctgac agcagaagct ctgaccagta tttctcagac
     4561 tacgctgcaa aacttaagca atctggaaaa aggtgaaacc tgcccgaacg aactggttta
     4621 atcttgcagc tcccctgcaa tccaggggag ctgattcaga taatcc
//
LOCUS       DAJTYH010000193         4620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTYH010000193 DAJTYH010000000
VERSION     DAJTYH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            109..4227
                     /gene="pic"
                     /locus_tag="OO609_003934"
     CDS             109..4227
                     /gene="pic"
                     /locus_tag="OO609_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR6635146.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGDTVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4378..>4620)
                     /locus_tag="OO609_003935"
     CDS             complement(4378..>4620)
                     /locus_tag="OO609_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR6635147.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1233 a         1047 c         1225 g         1115 t
ORIGIN      
        1 attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca ttagcttctt
       61 cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt gaataaagtt
      121 tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga acttgcccgc
      181 agggtaataa aaaagacatg ccgaagatta acgcatattc ttctggctgg cattccagca
      241 atctgtctgt gttactctca gatatcccag gcgggtattg tccgttccga tattgcctat
      301 cagatttatc gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc caatgatatt
      361 ccggtatatg ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc aatggccgat
      421 ttcagcagtg tgagctcaaa tggcgttgct acgcttgtat cgcctcagta tatcgtcagc
      481 gtaaagcata acggaggata tcggagtgtg agctttggta atgggaaaaa tacatattcc
      541 cttgttgacc gtaataacca cccttctatt gacttccatg ctccacgtct gaataaactg
      601 gttacagaag ttattccctc agcggtaaca tcagaaggaa ccaaagccaa tgcttataaa
      661 tacactgaac gttacaccgc tttttatcgg gtgggtagtg gtacgcagta cactaaggac
      721 aaggacggaa atttagttaa ggttgccggt ggatatgctt ttaaaacagg aggaaccaca
      781 ggagttcctc tgatatctga tgcaacaata gtctctaatc ccgggcaaac ttataatcct
      841 gtaaacggcc ctttacctga ctatggagcc cctggggata gtggttctcc tttgtttgct
      901 tatgataaac aacaaaaaaa atgggttatt gttgctgtat taagagcata tgcaggtatt
      961 aatggtgcta cgaactggtg gaatgtcata ccaacagatt atctgaacca ggttatgcag
     1021 gacgatttcg atgcccccgt agactttgtt tccggactgg gccccctgaa ctggacatac
     1081 gacaaaacat caggcacagg taccctgagc cagggcagta aaaactggac catgcacggg
     1141 cagaaagaca atgacctcaa tgccggtaaa aatctggtat tcagcgggca gaatggtgca
     1201 attatcctga aagacagtgt gactcagggt gccggttatc tcgaatttaa agacagttac
     1261 accgtatctg ctgaatccgg aaaaacatgg acgggtgccg gcattattac tgacaagggg
     1321 acgaatgtaa cctggaaggt caacggcgtt gccggtgaca acttgcataa gctgggggaa
     1381 ggaaccctga ccataaacgg aacaggtgta aacccgggag gactgaaaac gggagacggt
     1441 atcgttgtac ttaaccagca ggcagacact gcaggtaata tccaggcctt cagttcagtg
     1501 aacctcgcca gcggacgtcc gaccgtggtg ctcggggatg cccgtcaggt caatccggat
     1561 aacatttcat ggggataccg gggaggtaag cttgacctta atggtaatgc cgttaccttc
     1621 acccgactgc aggctgctga ttacggggcg gtgattacaa ataatgcaca gcaaaaatcc
     1681 cagcttttac tggatcttaa ggctcaggat acaaatgtca gtgaaccgac gattggaaat
     1741 atatccccct ttggtggtac cggcactcca ggaaacctgt acagcatgat actcaacagc
     1801 cagacccgct tctatattct gaaatctgcc agctatggta acactctgtg ggggaacagc
     1861 ctgaatgatc cggctcagtg ggagtttgtt ggcatgaaca aaaacaaagc agttcagaca
     1921 gtaaaagata ggatcctggc cgggcgggca aaacaacccg ttatctttca tggtcagctg
     1981 accgggaata tggatgtcgc cattccacag gtgccggggg gaagaaaggt aatctttgat
     2041 ggtagcgtga acctgccgga aggtaccctg agtcaggaca gtggcaccct gatattccag
     2101 ggacatccgg ttatccatgc ctccatcagt ggcagtgcac cggtcagcct gaaccagaaa
     2161 gactgggaaa accgtcagtt tacaatgaaa acactgtcgc tgaaagacgc tgacttccat
     2221 ctttcacgta acgcctcgct gaacagtgac attaagtcgg ataacagcca tatcacactg
     2281 ggaagtgaca gggcatttgt ggataaaaat gacggaacag gaaattatgt cattccggag
     2341 gaaggtacct ctgtcccgga caccgtgaat gacaggagcc agtatgaagg gaatattacg
     2401 ctgaaccata actcagccct ggatatcggc agcaggttca ccggggggat tgacgcttat
     2461 gacagtgccg tcagcatcac ctctccggac gtcctgttga cagccccggg tgcttttgcc
     2521 ggcagttcac tgacagtgca tgatggcggt catcttacag cactgaacgg tcttttcagc
     2581 gacgggcata ttcaggccgg taagaacggc aaaatcaccc tgagcggtac accggttaaa
     2641 gatacggcta atcagtatgc ccctgctgta tatctgacgg acggatatga cctgaccggc
     2701 gataacgcag cactggaaat tacccgtgga gcacatgctt ccggtgatat tcatgcctct
     2761 gcggcatcaa cagttaccat cgggtctgac acgccggcag aactggcttc tgcggaaacg
     2821 gctgcatcgg cgtttgccgg cagtcttctt gagggctata acgcagcatt caatggtgcc
     2881 ataaccggtg gcagggctga tgtcagtatg cataatgcac tgtggactct gggtggggac
     2941 tctgccatcc acagtcttac cgtcagaaac agccgtatta gttctgaagg agaccgtaca
     3001 ttccgtaccc tgacggtgaa taaactggat gcaacaggca gtgattttgt tttgcgtacg
     3061 gacctgaaaa atgccgataa aattaatgtg actgaaaaag ccactggttc agataacagc
     3121 ctgaacgtca gctttatgaa taatcctgct cagggacagg ccctgaatat tcctctggtc
     3181 acggcaccgg cgggaacttc agcagagatg tttaaggccg gcacccgggt gacaggtttc
     3241 agtcgggtga ccccaaccct gcatgttgat accagtggtg gcaatacgaa gtggatactg
     3301 gatggtttta aagcggaggc tgataaagcc gctgccgcga aggctgacag tttcatgaat
     3361 gccgggtata aaaacttcat gacggaagtt aacaatctga acaaacgtat gggtgacctg
     3421 cgtgacacaa acggtgatgc cggtgcctgg gcgcgcatca tgagtggtgc cggttctgca
     3481 gacggtggtt acagtgataa ttacacccat gttcaggtcg gctttgacaa aaaacatgaa
     3541 ctggacggtg tggacctgtt taccggtgtc acgatgacct ataccgacag cagtgcagac
     3601 agccatgcat tcagcggaaa gacgaaatcg gtggggggcg gtctgtatgc ttcagcattg
     3661 tttgagtccg gtgcctatat cgatttgatt ggtaaatata ttcaccatga caatgattac
     3721 acaggtaact ttgctagcct gggaacgaaa cactacaaca cccattcctg gtatgccggt
     3781 gctgaaacgg gttaccgcta tcacctgaca gaggacacgt tcattgagcc gcaggctgaa
     3841 ctggtttacg gcgccgtgtc cgggaaaaca ttccgctgga aagacggtga tatggacctg
     3901 agcatgaaga acagggactt cagtccgctg gttggaagaa caggggttga actgggcaag
     3961 accttcagtg gtaaggactg gagtgtgacg gcccgtgccg gaaccagctg gcagtttgac
     4021 ctgctgaata atggagatac cgtactgcgt gatgcgtccg gggagaaacg gataaaagga
     4081 gagaaggaca gccggatgct gtttaatgtt ggtatgaatg cgcagataaa ggacaatatg
     4141 cgctttggtc tggagtttga gaagtcagcc tttggtaaat ataacgtgga taatgcggta
     4201 aacgcgaatt tccggtatat gttctgatgt gggggtaaag ggctgccgga aggcagcctt
     4261 tctcagagac tcttaaaata taaaactgaa ggtcttcggt aagggggagt gtattagacg
     4321 tcaactagtt ttgccgactg gcgcgagcct gtttaagatt ctgtgtaaat gcctttctca
     4381 gaagtgaccg tccaggcggt caccgaactc gataataaag cggctcattg ccattcgcca
     4441 gtccttcaac ggcatcgtcc atttctggga cgcagactgg attgccagcc acaccacttt
     4501 tttcaccgag tcgtctgtcg ggaacacttt acgctttttg atcgcatggc ggatcacgct
     4561 gtttagcgac tcgatggcat tcgtcgtata gatcactttg cggatgtccg ttggataagc
//
LOCUS       DAJTYH010000194         4593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTYH010000194 DAJTYH010000000
VERSION     DAJTYH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="OO609_003936"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="OO609_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6635148.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="OO609_003937"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="OO609_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6635149.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="OO609_003938"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="OO609_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6635150.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4593)
                     /locus_tag="OO609_003939"
     CDS             complement(3930..>4593)
                     /locus_tag="OO609_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6635151.1"
                     /translation="NETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQ
                     LEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGL
                     ATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIA
                     FDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASR
                     SA"
BASE COUNT         1077 a         1294 c         1107 g         1115 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg tta
//
LOCUS       DAJTYH010000195         4577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTYH010000195 DAJTYH010000000
VERSION     DAJTYH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..575)
                     /locus_tag="OO609_003940"
     CDS             complement(<1..575)
                     /locus_tag="OO609_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR6635152.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
     gene            complement(690..765)
                     /locus_tag="OO609_003941"
     tRNA            complement(690..765)
                     /locus_tag="OO609_003941"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:ggt)
     gene            complement(772..846)
                     /locus_tag="OO609_003942"
     tRNA            complement(772..846)
                     /locus_tag="OO609_003942"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(811..813),aa:Gly,seq:tcc)
     gene            complement(963..1047)
                     /locus_tag="OO609_003943"
     tRNA            complement(963..1047)
                     /locus_tag="OO609_003943"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1011..1013),aa:Tyr,seq:gta)
     gene            complement(1056..1131)
                     /locus_tag="OO609_003944"
     tRNA            complement(1056..1131)
                     /locus_tag="OO609_003944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1096..1098),aa:Thr,seq:tgt)
     gene            1493..2443
                     /gene="coaA"
                     /locus_tag="OO609_003945"
                     /gene_synonym="panK"
     CDS             1493..2443
                     /gene="coaA"
                     /locus_tag="OO609_003945"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6635153.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2472..3437)
                     /gene="birA"
                     /locus_tag="OO609_003946"
     CDS             complement(2472..3437)
                     /gene="birA"
                     /locus_tag="OO609_003946"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6635154.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3434..4462)
                     /gene="murB"
                     /locus_tag="OO609_003947"
     CDS             complement(3434..4462)
                     /gene="murB"
                     /locus_tag="OO609_003947"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6635155.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1165 a         1155 c         1037 g         1220 t
ORIGIN      
        1 agttccagga ttttcgcttc ccactctgcg tcgccttcca gcgctttcag agcagaacca
       61 cgaacgatcg gagtgtcgtc gcccgggaag tcgtactgag acagaagttc acgaacttcc
      121 atttcaacca gttccagcag ctcttcgtca tcaaccatgt cgcatttgtt caggaacacg
      181 atgatgtacg gaacgcctac ctgacgaccc agcaggatgt gctcacgagt ctgcggcatc
      241 gggccgtcag tcgcagcaac taccaggatc gcgccgtcca tctgagcagc accggtgatc
      301 atgtttttaa catagtcggc gtgccccggg cagtctacgt gtgcgtagtg acgggtcggg
      361 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
      421 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      481 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      541 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      601 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      661 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      721 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      781 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      841 gcccgcatcc tagaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      901 ctctttattc gagccggtaa gtgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      961 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
     1021 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
     1081 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
     1141 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
     1201 tggtcacatt ttatgctaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
     1261 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
     1321 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1381 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1441 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1501 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1561 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1621 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1681 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1741 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1801 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1861 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1921 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1981 caaatccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     2041 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     2101 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ccgattttgt
     2161 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     2221 ttttctgaaa ttccgcgaag gggcttttac agacccggat tcctattttc ataactacgc
     2281 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     2341 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2401 aagtgctaat catgtggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2461 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2521 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2581 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2641 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2701 aattcacgta ttagcatggc cgccaacgta ttacgatcaa gattgatccc cgcttcctgc
     2761 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2821 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2881 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2941 gctcccagct ttcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     3001 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     3061 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     3121 gcatcgcccg atttaagctc tccgatacga tcaagaagga actgattcgt ggagtcaata
     3181 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     3241 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     3301 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3361 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3421 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3481 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3541 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3601 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3661 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3721 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3781 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3841 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3901 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3961 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     4021 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     4081 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     4141 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     4201 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     4261 tgatccggtt gatgatcacc gtgccgcgat agtcttccag aaaaagtaca ttacttcctt
     4321 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4381 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4441 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacgaggc tagtttaccg
     4501 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4561 ggcccgactg ggctttc
//
LOCUS       DAJTYH010000196         4527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTYH010000196 DAJTYH010000000
VERSION     DAJTYH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..2488
                     /gene="mutS"
                     /locus_tag="OO609_003948"
     CDS             <1..2488
                     /gene="mutS"
                     /locus_tag="OO609_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6635156.1"
                     /translation="EILLFYRMGDFYELFYDDAKRASQLLDISLTKRGASAGEPIPMA
                     GIPYHAVENYLAKLVNQGESVAICEQIGDPATSKGPVERKVVRIVTPGTISDEALLQE
                     RQDNLLAAIWQDSKGFGYATLDISSGRFRLSEPADRETMAAELQRSNPAELLYAEDFA
                     EMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFGTRDLVGFGVENAPRGLCAAGCLLQY
                     AKDTQRTTLPHIRSITMEREQDSIIMDAATRRNLEITQNLAGGAENTLASVLDCTVTP
                     MGSRMLKRWLHMPVRDTRVLLERQQTIGALQDFTAELQPVLRQVGDLERILARLALRT
                     ARPRDLARMRHAFQQLPELRAQLETVDSAPVQALREKMGEFAELRDLLERAIIDTPPV
                     LVRDGGVIASGYNEELDEWRALADGATDYLERLEVRERERTGLDTLKVGFNAVHGYYI
                     QISRGQSHLAPINYMRRQTLKNAERYIIPELKEYEDKVLTSKGKALALEKQLYEELFD
                     LLLPHLEALQQSASALAELDVLVNLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQV
                     LNEPFIANPLNLSPQRRMLIITGPNMGGKSTYMRQTALIALMAYIGSYVPAQKVEIGP
                     IDRIFTRVGAADDLASGRSTFMVEMTETANILHNATEYSLVLMDEIGRGTSTYDGLSL
                     AWACAENLANKIKALTLFATHYFELTQLPEKMEGVANVHLDALEHGDTIAFMHSVQDG
                     AASKSYGLAVAALAGVPKEVIKRARQKLRELESISPNAAATQVDGTQMSLLSVPEETS
                     PAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2594..3246
                     /gene="pphB"
                     /locus_tag="OO609_003949"
                     /pseudo
     CDS             2594..3246
                     /gene="pphB"
                     /locus_tag="OO609_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3297..4064)
                     /locus_tag="OO609_003950"
     CDS             complement(3297..4064)
                     /locus_tag="OO609_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6635157.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4260..4484
                     /locus_tag="OO609_003951"
                     /pseudo
     CDS             4260..4484
                     /locus_tag="OO609_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1103 a         1172 c         1186 g         1066 t
ORIGIN      
        1 cgagatcctg ctgttttacc ggatgggtga tttttatgaa ctgttttatg acgacgcaaa
       61 acgcgcgtcg caactgctgg atatttcact gaccaaacgc ggtgcttcgg cgggagagcc
      121 gatcccgatg gcggggattc cctaccatgc ggtggaaaac tatctcgcca aactggtgaa
      181 tcagggagag tccgttgcca tctgcgaaca aattggcgat ccggcgacca gcaaaggtcc
      241 ggttgagcgc aaagttgtgc gtatcgttac gccaggcacc atcagcgatg aagccctgtt
      301 acaggagcgt caggacaacc tgctggcggc tatctggcag gacagcaaag gtttcggcta
      361 cgcgacgctg gatatcagct ccgggcgttt tcgcctgagc gaaccggccg accgcgaaac
      421 gatggcggca gagctgcaac gctctaatcc ggcggaactg ctgtatgcag aagatttcgc
      481 cgagatgtcg ctgattgaag gccgtcgcgg cctgcgccgt cgcccgctgt gggagtttga
      541 aatcgacacc gcgcgccagc agttgaatct gcaatttggc acccgcgatc tggtcggttt
      601 tggcgtcgag aacgcgccgc gcggactttg tgctgccggt tgtctgttgc agtatgcgaa
      661 agatacccaa cgcacgaccc tgccgcatat tcgttctatc accatggaac gtgagcagga
      721 cagcatcatt atggatgccg cgacacgtcg taacctggaa attactcaga acctggcggg
      781 cggtgcggaa aatacgctgg cttctgtgct cgactgcacc gtcacgccga tgggtagtcg
      841 tatgctgaaa cgctggctgc atatgccagt gcgcgatacc cgcgtgttgc ttgagcgcca
      901 gcaaactatt ggcgcattgc aggatttcac cgccgagttg cagccggtac tgcgtcaggt
      961 cggcgacctg gaacgtattc tggcacgtct ggctttacga actgctcgcc cacgcgatct
     1021 ggcccgtatg cgccacgctt tccagcaact gccggagctg cgtgcgcagt tagaaactgt
     1081 cgatagtgca ccggtacagg cgctacgtga gaagatgggc gagtttgccg agctgcgcga
     1141 tctgctggag cgagcaatca tcgacacacc gccggtgctg gtacgcgacg gtggtgttat
     1201 cgcatcgggc tataacgaag agctggatga gtggcgcgcg ctggctgacg gcgcgaccga
     1261 ttatctggag cgtctggaag tccgcgagcg tgaacgtacc ggcctggaca cgctaaaagt
     1321 tggctttaat gcggtgcacg gctactacat tcaaatcagc cgtgggcaaa gccatctggc
     1381 acccatcaac tacatgcgtc gccagacgct gaaaaacgcc gagcgctaca tcattccaga
     1441 gctaaaagag tacgaagata aagttctcac ctcaaaaggc aaagcactgg cactggaaaa
     1501 acagctttat gaagagctgt tcgacctgct gttgccgcat ctggaagcgt tgcaacagag
     1561 cgcgagcgcg ctggcggaac tcgacgtgct ggttaacctg gcggaacggg cctataccct
     1621 gaactacacc tgcccgacct tcattgataa accgggcatt cgcattaccg aaggtcgcca
     1681 tccggtagtt gaacaagtac tgaatgagcc atttatcgct aacccgctga atctgtcgcc
     1741 gcagcgccgt atgttgatta ttaccggccc gaacatgggc ggtaaaagta cctatatgcg
     1801 ccagaccgca ctgattgcgc tgatggccta catcggcagc tatgtaccgg cacaaaaagt
     1861 cgagattgga cctatcgatc gcatctttac ccgcgtaggc gcggcagatg acctggcttc
     1921 cgggcgctca acctttatgg tggagatgac tgaaaccgcc aatattttac ataacgccac
     1981 cgaatacagt ctggtgttaa tggatgagat cgggcgtgga acgtccacct acgatggtct
     2041 gtcgctggcg tgggcgtgcg cagaaaatct ggcgaataag attaaggcat tgacgctgtt
     2101 tgccacccac tatttcgagc tgacccagct accggagaaa atggaaggcg tcgccaacgt
     2161 acatctcgat gcgctggagc acggcgacac tatcgccttt atgcacagcg tgcaggacgg
     2221 cgcggcgagc aaaagctacg gtctggcggt tgctgctctg gcgggcgtac cgaaagaggt
     2281 tattaagcgc gcacggcaaa aactgcgtga gctggaaagc atttcgccga acgccgccgc
     2341 gacgcaagtg gatggtacgc agatgtcttt gctgtcagtg ccagaagaaa cttcgcctgc
     2401 ggtcgaagca ctggaaaatc tcgacccgga ttcactgact ccgcgtcagg cgctggagtg
     2461 gatttatcgc ttgaagagtc tggtgtaata acaattcccg atagtctttt gctatcggga
     2521 atattaacga caactgacga atcaaataaa aataccctgt ataataggaa agcttatttt
     2581 acagggtaaa accatgccat ctacacgcta ccaaaaaatc aatgcccatc actatcgcca
     2641 tatatgggtc gttggtgata ttcatggtga atatcagtta ttacaatccc gcttacatca
     2701 actctctttt ttccccgaaa ccgacttact tatttctgtc ggcgataata ttgatcgtgg
     2761 gccggagagt cttgacgtcc tgcgcctgct aaaccagccc tggtttatct ccgttaaagg
     2821 caaccacgaa gcaatggcgc tggatgcgtt cgcgaccggc gatggcaata tgtggcttgc
     2881 cagcggtggt gactggtttt tcgatttaaa tgattgcaac aagaggcaat agttctgttg
     2941 ctgaaattcc atcaccttcc acatattatt gaaatcacta acgacataat aaaatatgtc
     3001 atcgcacatg cagattatcc gggtgatgaa tatctctttg gcaaagaaat agcggagagc
     3061 gaattactct ggcctgttta tcgtgtgcag aaatcgctta atggcgagtt acaacaaata
     3121 aacggcgctg attattttat atttggacat atgatgtttg ataacattca gacgttcgct
     3181 aaccagattt atattgatac cggatcgccg aaaagtgggc ggctgtcatt ttataaaata
     3241 aggtagtctc atgcttcttc tgtgaagcat gagtaaccag atgttatttc aggccattat
     3301 tcatttttcg ctaccagcaa agagagatcc tgcttcgcca gcgcgcgact ggcactctcc
     3361 ggcaaaccgt cgtctgtaat aatctgatca aactcgctta atggtaacgc cagccatgtc
     3421 gccacctgac catatttcgt cgcatcacag accaaaactc gctggcggct ggcactggca
     3481 atcgcccgtt tcaccgtgac tttatcttcc gctggcgtag aaatcccccg cacactccat
     3541 gacgatgcag aaataaaagc ctgatcaatc atcaggctgc acagcatggt cgcagcggct
     3601 tccccgacac aggaacggtt ttcccgacac actgcaccgc cagtgtgaat aattgtgcaa
     3661 ttactgttgt cgagcaagta gttcgcaata acgaaatcgt ttgtgaccac agtcagtgac
     3721 tccatgtgaa tcagatgctg tgctatcgct aacgtggtcg ttcccgcatc cagatagata
     3781 caacttcccg gcagaacaag acttgccgcc agcttgccaa tagccgcttt ttgcgtcatt
     3841 gccagcgcag tttttacctg atgagaaggt tcatgcgcca cgcgtcccgg agactggacg
     3901 cctccggaca ccagcacaac ggctccctgc tgctccagtt tttgtaaatc ccgacgaatg
     3961 gtcatatgtg acacattaat tctgtccgtt agttcagcaa tactgacaat gcctttttca
     4021 gctaccatct caaggatgat ttggcgacgc tctacgggta tcaacttttg ctccttcctt
     4081 tgtcctgctg acattctacg ctatttgcct gcgaaacgtg cgcggcgcaa ctaacgctta
     4141 gttcacataa aataacacaa aatgttaatt tatgtgaatc atctcaccat atcgttatct
     4201 cccagcgctt atgttcacaa tatcaaacaa aatatcactt aaattaacaa ggagagcaga
     4261 tgaaaacggg atctgagttt catgtcggta tcgttggctt agggtcaatg ggaatgggag
     4321 cagcactgtc atgtgtccgc gcaggttttt ctacctgggg cgcagacctg aacagcaatg
     4381 cctgcgctac gttgaaagag gcaggtgctt gcggggtttc tgataacgcc gcgacgtttg
     4441 ccgaaaaact ggacgctctg ctggtgctgg tggtcaatgc ggcctaggtt aaacaggtgc
     4501 tgtttggtga aacaggcgtt gcacaac
//
LOCUS       DAJTYH010000197         4456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTYH010000197 DAJTYH010000000
VERSION     DAJTYH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..517
                     /locus_tag="OO609_003952"
     CDS             <1..517
                     /locus_tag="OO609_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6635158.1"
                     /translation="RAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITLLNQ
                     AVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFIN
                     QLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAV
                     LIEAMESRSLR"
     gene            714..2153
                     /gene="aldA"
                     /locus_tag="OO609_003953"
     CDS             714..2153
                     /gene="aldA"
                     /locus_tag="OO609_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6635159.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2195..3195)
                     /gene="gap"
                     /locus_tag="OO609_003954"
                     /pseudo
     CDS             complement(2195..3195)
                     /gene="gap"
                     /locus_tag="OO609_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3384..3914
                     /gene="cybB"
                     /locus_tag="OO609_003955"
     CDS             3384..3914
                     /gene="cybB"
                     /locus_tag="OO609_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6635160.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4159..4332
                     /locus_tag="OO609_003956"
     CDS             4159..4332
                     /locus_tag="OO609_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635161.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
BASE COUNT         1141 a         1028 c         1117 g         1170 t
ORIGIN      
        1 cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata ttccgcatga
       61 aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct ttagcatcac
      121 gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg accccaccat
      181 gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg atgcaccacg
      241 ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca gtgtaatctt
      301 tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac tcactggcga
      361 gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag attttatcgt
      421 tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac atgatgcggt
      481 gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt gtgaaccact
      541 tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct tttacacttg
      601 tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg atgattgatg
      661 ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg cccatgtcag
      721 tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt ggagacgcat
      781 ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc gatggtcagg
      841 ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt
      901 tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc cgcgaacgcg
      961 ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag ctagctgaag
     1021 tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca cggcgttacg
     1081 agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt aaacgtgcgc
     1141 ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt gcccgcaaaa
     1201 tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa tttacgccaa
     1261 acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc ggcgtgttta
     1321 accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac ccaaaggtcg
     1381 caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg actgcggcga
     1441 aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc gtaatggacg
     1501 atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt aatagtgggc
     1561 aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat cagttcgtca
     1621 atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa cgcaacgaca
     1681 ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa aaagtggcgc
     1741 gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag gggaaaggat
     1801 attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt atgcatgagg
     1861 aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat gctatctcaa
     1921 tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat ctgaacgtcg
     1981 cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt gaaaacttcg
     2041 aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc gcagatggta
     2101 aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct taatgagtga
     2161 aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat ttttcgaggg
     2221 tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc gttttcacca
     2281 gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact gaaccgaaat
     2341 ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat gactcgttat
     2401 tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt ttccgagaat
     2461 ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat gacctttcag
     2521 tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg tagtgtgggg
     2581 aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg ggccatccac
     2641 cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt ctatcccgaa
     2701 actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg acgccacgga
     2761 aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga tggttttcat
     2821 ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat gcgcctgcga
     2881 tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc ctttcgcttt
     2941 ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc catcaacgat
     3001 aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg aatcatgttt
     3061 cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa cgtctatgtt
     3121 gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa aaccgttaat
     3181 accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt gatcctttcc
     3241 aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa cgtaacgtac
     3301 tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg ttaacggaaa
     3361 attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa atcagcattc
     3421 actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt cgtggtttct
     3481 tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc atctcaattc
     3541 tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg ccgattatac
     3601 ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg atttatctcc
     3661 tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc aacccgtggt
     3721 ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg gttgatagct
     3781 taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg ttgcacgctg
     3841 cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc atgatgccgc
     3901 gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac ccgtggtaca
     3961 ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat gacaaataaa
     4021 accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac ccgatctttg
     4081 atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac ataagattcg
     4141 ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg aacgcttgtt
     4201 tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg gatctcacac
     4261 tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc gaaaaaaaca
     4321 tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag tcagatgcgc
     4381 ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc agcaacctcc
     4441 ttatcaccgt cccgga
//
LOCUS       DAJTYH010000198         4428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTYH010000198 DAJTYH010000000
VERSION     DAJTYH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO609_003957"
     CDS             complement(263..1120)
                     /gene="sitD"
                     /locus_tag="OO609_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6635162.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO609_003958"
     CDS             complement(1117..1974)
                     /gene="sitC"
                     /locus_tag="OO609_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6635163.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO609_003959"
     CDS             complement(1971..2798)
                     /gene="sitB"
                     /locus_tag="OO609_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6635164.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO609_003960"
     CDS             complement(2798..3712)
                     /gene="sitA"
                     /locus_tag="OO609_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6635165.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4144..>4428)
                     /locus_tag="OO609_003961"
     CDS             complement(4144..>4428)
                     /locus_tag="OO609_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6635166.1"
                     /translation="FMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHTF
                     ACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1189 a         1078 c         1056 g         1105 t
ORIGIN      
        1 tgaaggaaga gctgaatagt ctgaaggatt tgaattcggt taggaatttt atcgtggaat
       61 aacagaagct gcggcacgtc gtatgcagga acgtgccgcg gttggctggt aaactttcga
      121 tagtgcgagt attgaatgat ttctaggcgt tatcgatttt acgtattttt tgcatgatag
      181 aattcgtacc tcctcccaac gaccttccat ggctttccgg acttctgtag gctggcggac
      241 gatgctgtcc tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc
      301 cctttgtgcg tgcgttgatg ctggtaacgg taaacgtcat gatgaacacg accgcgaata
      361 agacgacaat cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccagtca
      421 ttgacactat taccgatatg ccagtcgcca gtaggagcgc aatatgaaag cgttgtgtca
      481 ggagtactgc aatggcgcca ggcgcaatga gcagagaaat agagagaatg atacctactg
      541 ctttcagcgt cgccacaatg gtcagggaaa ccatgcacag cagcccataa tgcagccatc
      601 tcgtatgcaa accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc
      661 gccattttac gctaataaca agtgtaacca gcccggcaat aatcaccgtc tggattatgt
      721 cgccgatggt tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt
      781 taattgcaat atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccataa
      841 ctgtatcttg cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata
      901 ctccggcgac gaatgcccct gtagccagag gtaaccccag aatccaggta agcacgatgc
      961 caggaaaaac cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa
     1021 agaccgacag cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca
     1081 taaaggtgaa ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt
     1141 atgcgcacgc cgacgatttg ccaaaagtcc atgggtgggc gcaaatacaa atgctaaaag
     1201 aaacagcagt gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact
     1261 gacccacgcg ccgaggaaac tggttacact gccaatagtt acagcaatta tgagcaggcg
     1321 gggaaaacga tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac
     1381 cagaaaggca ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa
     1441 gaggattttt aatctgcccg gatgtaatcc aatagcacgg gcgtgattct cgtcgaaaaa
     1501 ggtcaccata agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat
     1561 agtgagttgc aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg
     1621 aatgttcacg gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga
     1681 gaagatcagg ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat
     1741 gctgcccgct gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat
     1801 atatgccccc gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca
     1861 gccttttagc atcagatagc atgacaggaa agcgcagaga ccgccgacca ttgccgagac
     1921 ccacatcgca ttgagcatat attcatagct gaagggttcc agtagcacgt tcatcttgct
     1981 tccctctgca ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg
     2041 ctttcttccg agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg
     2101 ttctcggctg taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg
     2161 accgtataat cgcaaaatgt cgtgactgag ccgagattgt gcgttgagac aagcatcgtt
     2221 ttgccttccg cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca
     2281 acgccggtaa aaggttcatc cagcaggatc acatcaccct gctgtgcaat tgctctcgcc
     2341 agaaagacgc gtttcttttg tccaccggaa agctcgccga tttgacgatg gcgaaaatcc
     2401 accatatcga cacgttccag cgcatcggtg acaatctgac gatccctctt tttggctata
     2461 cgcaacatac ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga
     2521 aatgaccagt caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc
     2581 tgtcgcgtgg gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata
     2641 atcgctttaa acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat
     2701 ccacttggta ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca
     2761 gtaacatcat taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt
     2821 taattccctg taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtaggtaccg
     2881 ggccgttttc tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt
     2941 cacgcgcaac ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga
     3001 tatgattttt cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat
     3061 cggcattaat cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa
     3121 aagccccttc actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac
     3181 gcaggggggc aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg
     3241 tttgtgcatt tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca
     3301 gagcattatc tggcgacatc catgcatggg gattaggttt gccctcatag ggtccttcgg
     3361 tgatccctac tggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct
     3421 ggtaaaagcg ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc
     3481 cctgcgcacg tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct
     3541 tggttatgga tgagacttct gcagcatctc cagccacgtt tttggccata tccgcgatga
     3601 tggtaaatgt tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc
     3661 aggtgagggc gaggcacccc aagagcatgg ttactttttt tattgagtgc ataatttagt
     3721 acctatagtg agcaaaatta gaacaaacat agcaacggct atgttttata gcataaccta
     3781 tacaaaatga aaataattat cattaacagc tttgaggcct aaaacaggga ttaggttgtt
     3841 tcctgctggc gacatgatat tgaggaaaaa agtagagcag ctattaggct ttgaaatgac
     3901 tttatgagga aaattgttga tggcaaaaat ggggcaaaaa agagtgggtt atcgtagctt
     3961 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgagtcaga
     4021 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttctttg taatttgttg
     4081 attttcttgc attatttcag ttctctggta ctaaatgggg caaattgggg gcaaactttg
     4141 caactacgat aaccgcgcat tcaacatggc tatctgttcg tcgttcatgt catcaatcca
     4201 cataccgtaa atttcataca ccatttgcgc agtttcatgc cccatctggc tggctataaa
     4261 tgccgggttc gctcctgccg tcaacagcca gcaggcaaaa gtatgtcgcg tatggtacgg
     4321 attacggcgg cgaataccag cacgttttac tgctgcattc catcttgtac ccaaactgct
     4381 taccgagtaa taaggtttct gtttttcgtt acacaccctg ggcatgaa
//
LOCUS       DAJTYH010000199         4325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTYH010000199 DAJTYH010000000
VERSION     DAJTYH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..177)
                     /locus_tag="OO609_003962"
     CDS             complement(<1..177)
                     /locus_tag="OO609_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6635167.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRLFLPEGTLGQW
                     VTAARKGLGTPGSRT"
     gene            203..466
                     /locus_tag="OO609_003963"
                     /pseudo
     CDS             203..466
                     /locus_tag="OO609_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            512..721
                     /gene="hha"
                     /locus_tag="OO609_003964"
     CDS             512..721
                     /gene="hha"
                     /locus_tag="OO609_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6635168.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            760..1350
                     /locus_tag="OO609_003965"
     CDS             760..1350
                     /locus_tag="OO609_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6635169.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1590..1850
                     /gene="repA"
                     /locus_tag="OO609_003966"
     CDS             1590..1850
                     /gene="repA"
                     /locus_tag="OO609_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6635170.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            2074..2148
                     /gene="tap"
                     /locus_tag="OO609_003967"
     CDS             2074..2148
                     /gene="tap"
                     /locus_tag="OO609_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6635171.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            2141..2998
                     /gene="repA"
                     /locus_tag="OO609_003968"
     CDS             2141..2998
                     /gene="repA"
                     /locus_tag="OO609_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6635172.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3699..3890
                     /locus_tag="OO609_003969"
     CDS             3699..3890
                     /locus_tag="OO609_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635173.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(4120..>4325)
                     /locus_tag="OO609_003970"
     CDS             complement(4120..>4325)
                     /locus_tag="OO609_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6635174.1"
                     /translation="VYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGL
                     KRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT         1151 a         1081 c         1065 g         1028 t
ORIGIN      
        1 cgtgcgggaa ccaggagtac cgagcccttt tctggcggcg gtaacccatt gtcctaaagt
       61 gccttcagga aggaataatc gggaagcgtc ttcactgatc gaaagttgat tttcaagaac
      121 cgttctgaca gcttcggctt tgaactcttt agagtaacgt cgggtttttc tgctcattat
      181 tagctccttc tgatgccatt ctatttcagg aaggagtgtc cgttaaactc aggctacctc
      241 atattttcag cgagatcgtt atggtcgtat tctcgggcag gtttatgcac cagacggaat
      301 gaatattaat cagtttatgg tccgggccgg ggctgcctgg gtatatgagc agtacaacac
      361 agatcccgtg ctacctgtcc ttcagaacga agccagacaa caaaaacgtg ggctctggtc
      421 agatgctgat ccggttcccc cctggatatg gaggcaccgt aaataaagcg atggcaatcc
      481 tatcattcat ctgttctcag cgggaataaa aatggcaaaa acaaaacagg aatggctgta
      541 tcagttacgg cgatgttcat cagtcaatac gctggaaaaa attattcata aaaacagaga
      601 cagtttatca aacagtgaac gtgaatcatt taattcagca gcagaccacc ggctagcgga
      661 attaatcacc ggaaagttat atgacaggat accaaaagag atatggaaat atgtaaggta
      721 ataattcagc aataattaat tgccaggagc agataaataa tgaagttgat tatttttatt
      781 ctcattgttc tgattattgc agcgctactt atccggatta tcctcagaag tgtaaatcag
      841 cattctcctt tgctgatgca attgcatgcg gcaggtatac ggactggtga tgcagagcga
      901 atactgtcca gcggtgaata ctggcaacgt cagaagacgc tgctgacaga aagggaagtc
      961 agttttatga aaggactgtt cagaattgtg gatatgaagc ggtggtatct gtgtccgcag
     1021 gtacgggtag cggatatcgt ccagctgaac gggaatatcc ggccacgatc acgccagtgg
     1081 tggcagttat tcaggatggt gtctcagtgg catgttgatg tggttatcgt tgagctgcgt
     1141 tcgttcagta ttgttgcagc agtagagctg gatgatgcca gccatttacg accggaacgc
     1201 agacgccggg atattcttct ggaagaggtt ctgaggcagg ctggtattcc gttgctcaga
     1261 agccacgatg ccagaaaact gctgcagatg accggagaat ggctgaatac aacaggggct
     1321 gatcagcagt caccggaaca tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc
     1381 ggaaacggga aaaagcaagt tttcctcgct cccggcgttt ccataactga aaaccatact
     1441 atttcacagt ttaaatcaca ttaaacgaca gtaatccccg ttgatttgtg cgccaacaca
     1501 gatcttcgcc acaattctca agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac
     1561 gatccctgtt tgagtattga ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct
     1621 cattgagtca aaagcggttt gtgcgcagag gtaagcctat gactgactct gagaaacaaa
     1681 tggccgctgt tgcaagaaaa cgtcttacac acaaagagat aaaagttttt gtcaaaaatc
     1741 ctctgaaaga tctcatggtt gagtactgcg agagagaggg gataacacag gctcagttcg
     1801 ttgagaaaat catcaaagat gaactgcaga gactggatat actaaagtaa agactttact
     1861 ttgtggcgta gcatgctaga ttactgatcg tttaaggaat tttatggctg gccacgccat
     1921 aaggtggcag ggaactggtt ctgatgtgga tgtacaggag ccagaaaagc aaaaaccccg
     1981 ataatcttct ttaactttgg cgagtcagaa agattaccgg ggcccactta aaccgtatag
     2041 ccaacaatca agctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt
     2101 ctgtgctcgc tccttctgcg cattgtaagt gcaggatggt gtgactgatc ttcaacaaac
     2161 ttattaccgc caggtaaaga acccgaatcc ggtgttcaca ccccgtgaag gtgcccggac
     2221 gcttccgttc tgcgggaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt
     2281 cgccattcat gtggcacatg cccgttcgct tggtctgcgt cggcgcatgc caccggtact
     2341 acgtcgccgg gccattgatg cgctgctgca ggggctgtgt tttcactatg acccactggc
     2401 caaccgcgtc cagtgttcca tcaccacgct ggccattgag tgcggactgg cgacggagtc
     2461 tgctgccgga aaactctcca tcacccgggc cacccgggcc ctgacgttcc ttgcagagct
     2521 gggactgatt acctaccaga cggaatatga cccgcttatc gggtgctaca ttccgaccga
     2581 tatcacgttc acatctgcac tgtttgctgc cctcgatgta tcagaggagg cagtggccgc
     2641 cgcgcgccgc agtcgtgtgg aatgggaaaa cagacagcgc aaaaagcagg ggctggatac
     2701 cctgggtatg gatgaactga tggcgaaagc ctggcgtttt gttcgtgagc gtttccgcag
     2761 ttaccagaca gagcttaagt cccgtggaat gaagcgtgcc cgtgcgcgtc gtgatgcgga
     2821 caggcagcgt caggatattg tcaccctggt gaaacggcaa ctgacgcgtg aaatctcgga
     2881 agggcgcttc actgccagtc gtgaggcggt aaaacgtgaa gtggagcgtc gtgtgaagga
     2941 gcgcatgatt ctgtcacgta accgtaatta cagccggctg gccacagctt ccccctgaaa
     3001 gtgacctcct cagaataatc cggcacgcgc cggaggcatt cgcacgcctg aagcccgcca
     3061 gtgcacaaaa aatcagcgct acatacaaaa aacaacctca tcatccacct tcaggtgcat
     3121 ccggtccccc ctgtttttga tacaaaacac gcctcacaga cggggaattt tgcttatcca
     3181 cattaaactg caagggactt caccataagg ttacaaccgt tcacgtcata aagcgccaga
     3241 cgtcaaccct acagggtgca atgtatcttt taaacacctg tttatatctc ctttaaacta
     3301 cttaattaca ttcatttaaa aagaaaacct gttcactgcc tgtcctgtgg acagacaaat
     3361 atgcacctcc caccgcaagc ggcgggcccc caaccgaagc cggtttagtt acaacatgca
     3421 aacataaacc cctaaaaaac ccaagtccaa cgcaaaactg aaaccacaac gcccccctca
     3481 taactgaaaa gcgccgccgc ccccgcccaa agggcgggaa cagagtcgct tttaattatg
     3541 aatgttgtaa ctacatcatc atcgctgtca gtcttctggc tggacgtatc gaatacacgc
     3601 tcgtaagcgg ccctcacggc ccgctaacgc ggagatacgc cccgacttcg ggtaaaaccc
     3661 tcgtcgggac cactccgacc gcgcacagaa gctctatcat ggctgaaaac gggtatggct
     3721 tggcagggct ggggatgggt aaggtgaaat ctgtcaatca gtaccggctg acgccgggct
     3781 tcggcggttt tactccggta tcacatgtaa ccacagcgtg ccgccttcca tgccgctggc
     3841 gcggcatcag aataatacaa gcagcattta atgcatttgc aaaagtgtga gtaatattaa
     3901 gtggtcgttt cagggatatc gttaagactt gagcgatagg aattaaaacc ccaaaaagat
     3961 taaaaaaaca ccacaaaacg gatgtttctt caacaccact tttgctccat atgaacggaa
     4021 ccgacgatta aactggatgg ctctgattga ttcagggtat gaatggcggt tttttgctcc
     4081 gtttccctca aaatggacgc aacttcccct ctgcggctct cagccgcacc accgcatccg
     4141 ggccagcggc tcatgcatca ggacctgctc tgccagacgg tagccccgct tcagccccgt
     4201 aaaacgcatc tgactcccgc acagcacgca cttcagcggg tcaaccttca gtaacctctg
     4261 atacatccct ctccaggtga tttgcatcgc cgtttttctc actgtctccg ttatgatgta
     4321 cacca
//
LOCUS       DAJTYH010000200         4296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTYH010000200 DAJTYH010000000
VERSION     DAJTYH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..385
                     /locus_tag="OO609_003971"
     CDS             <1..385
                     /locus_tag="OO609_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635175.1"
                     /translation="VVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYL
                     SGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSA
                     TYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            719..2998
                     /gene="ydeP"
                     /locus_tag="OO609_003972"
     CDS             719..2998
                     /gene="ydeP"
                     /locus_tag="OO609_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6635176.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3245..3448
                     /gene="safA"
                     /locus_tag="OO609_003973"
     CDS             3245..3448
                     /gene="safA"
                     /locus_tag="OO609_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6635177.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3516..4277
                     /gene="ydeO"
                     /locus_tag="OO609_003974"
     CDS             3516..4277
                     /gene="ydeO"
                     /locus_tag="OO609_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6635178.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1210 a          931 c          987 g         1168 t
ORIGIN      
        1 tgttgttatg ccaacaggcg gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc
       61 ggactttcct ggaagcgcag aaatacccct tggcgtatat tgctcaagcg aacaaaagct
      121 ctcattttat ctttcgggtg ccaccactga cagtagccga caagtgtttg ctaacacagc
      181 acctgatgca acaaaagcat cgggagtggg tgtcactcta atgcgtaatg gaaaaatact
      241 ggctacggga gaaaatgtct ccctggggac agtaaataaa agcaaagtac ctctgggttt
      301 aagcgcaacc tatggacaaa caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat
      361 tggtgtcact tttatctatg aataattgtg attttgacct tagtctatgc gctccattca
      421 cgaaaattgc gcggataatt attttgtgaa ggctattatc ctacacctgt aagaaaatcc
      481 gcgcacaatt gaagccagag tcttattttt tatattggaa aatatgttaa tttaaacact
      541 atgagagatg atgcttaact tatcgtattt aatctattgt ttaacgataa tagattaaaa
      601 atagaaagag agtagtccct tactcttata tatccatgtt ggcgataatc cctttgtatg
      661 tactgtgcat catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat
      721 gaagaaaaaa attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt
      781 agcgaatgca gtacgtaagc agatggatat acgccatgat gttattgcca tgtttgacat
      841 gaataagcca gagggctttg actgtccggg ttgtgcatgg ccagatccta agaacagtgc
      901 gtcattcgac atttgtgaaa acggcgcaaa agcaatcgct tgggaagtca cggataagca
      961 ggtaaatgcc tctttctttg ctgagaatac ggttcaatca ttacttacct ggggagatca
     1021 cgagcttgag gctgcggggc gtctcactca gcctttgaaa tatgatgccg tcagcgactg
     1081 ttacaagcca ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta
     1141 tagtgatccc aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt
     1201 tctttatcag ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat
     1261 gtgccatgaa ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt
     1321 gttgctggaa gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac
     1381 aaaccaccct cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat
     1441 cgccatcaat cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt
     1501 tgaaatgctg acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg
     1561 tggcgatatg gcgttgctca aggggatgat gcgcctgtta attgagcgcg atgatgctgc
     1621 aagtgccgca ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg
     1681 ctttgacgag ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc
     1741 tggactaagt cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac
     1801 cattatctgt tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtgcagca
     1861 actggtcaat ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc
     1921 actacgtggt cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc
     1981 tgcagagttt ctggctcgcc tgggtgagcg ctatggcttc cccccacctc atgcacctgg
     2041 acatgctgca attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg
     2101 catggggggc aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac
     2161 gcaattagat ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc
     2221 acggcatagc tatattctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg
     2281 tgcgcaggcg gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt
     2341 aaaacccgcc ggtgtaatgc tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc
     2401 agcactaccc cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg
     2461 caatgacatt gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc
     2521 cggtggtttt cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa
     2581 ggctaatttc attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa
     2641 gctggtcatg gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga
     2701 tgatcgctat cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc
     2761 taaaatttgc cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg
     2821 taagcgcagc tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg
     2881 ctcactggtg acctattttc cagaatcgaa tcacatgcta acacttgata accacgatcc
     2941 attaagtggt attcctggct ataaaagtat tccggttgaa ttagaaccat caaattaatg
     3001 cctcttctca tttcttctgc tgtcatccgc acagcagaag aattcctcat tgactattat
     3061 tgcgcaattt gctcacatgg attaaattaa actacatact ataagatata aacttctgcc
     3121 tacagctgta agaaactccg ctcagtactg aagcaccagt cctatttctt cttttctcca
     3181 gcctgttata ttaagcatac tgattaacga cttttaacgt tatccgctaa ataaacatat
     3241 ttgaatgcat gcgaccacag tgaaaaacaa aatcacgcaa agagataact ataaagaaat
     3301 catgtctgta attgtggttg tcttattact gacacttacg ttgatagcca ttttttcggc
     3361 aattgatcag ctgagtattt cagaaatggg tcgcattgca agagatctta cacatttcat
     3421 tatcaatagt ttgcaagact ggaaataaac agcaaattat aaatgtgaaa tgtttaaaag
     3481 gtatcgataa aactttattg ttttaaggag ataaaatgtc gctcgtttgt tctgttatat
     3541 ttattcatca tgctttcaac gctaacattt tagataaaga ttacgccttc tccgacggcg
     3601 agatcctgat ggtagataac gctgttcgta cacattttga accttatgag cggcatttta
     3661 aagaaatcgg atttaatgaa aataccatta aaaaatatct acaatgcact aacatccaga
     3721 cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg actggattgc
     3781 ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat ttttcagaac
     3841 ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa aggtttcatt acactattaa
     3901 ctaacggtgt gctatccgtt tccgggaaag tgagaaatat tgtcaacatg aagctggcgc
     3961 acccatggaa gctgaaagat atttgtgact gcctgtacat cagtgaaagc ctgttgaaga
     4021 aaaaactaaa gcaagagcaa acgacattct cacagattct attagatgcc agaatgcagc
     4081 acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa caatgtggtt
     4141 atgccagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac agtccgaaga
     4201 gagtttctaa ggagtaccgt tgtcaaaggc acacgggtat gaatacgggc aacacgatga
     4261 gtgctttagc tatttgatta tttgctaacg agtagt
//
LOCUS       DAJTYH010000201         4224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTYH010000201 DAJTYH010000000
VERSION     DAJTYH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            102..1190
                     /locus_tag="OO609_003975"
     CDS             102..1190
                     /locus_tag="OO609_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6635179.1"
                     /translation="MSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLI
                     FVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSF
                     EATFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRL
                     KMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQ
                     VRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKY
                     LTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRR
                     GDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1190..1924
                     /locus_tag="OO609_003976"
     CDS             1190..1924
                     /locus_tag="OO609_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6635180.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2391..3152
                     /locus_tag="OO609_003977"
     CDS             2391..3152
                     /locus_tag="OO609_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6635181.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3196..>4224
                     /locus_tag="OO609_003978"
     CDS             3196..>4224
                     /locus_tag="OO609_003978"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6635182.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHE"
BASE COUNT         1160 a          811 c         1038 g         1215 t
ORIGIN      
        1 ttgcgagctg gctggcaaca ggttgcggcg tagcgttgta tctggcttta acggcgcgta
       61 ctcgttctct gacgttgata tgtttgttgt cgttataccc tctgtctaca gctttagtcc
      121 ttacacaaag ccgtgtcggc cttcttggtt tattttgcgt catcgtgctg gccgctctcg
      181 cggacagacc ccgctggcgc ggacgtcccc ttagcggtct gttgcgcttg atcttcgtcg
      241 cctccttgct gtgctggtgt gtcggtatct caaactatgc catgccaggg gagacaacgg
      301 ctgactttac gcattcatca tcgaatgacc agcgtatacg tattctgaaa ggtgccataa
      361 gtcttattca acagcgcccc ttaaccggtt gggggttagg cagctttgaa gcgaccttcc
      421 cgcaagcctt agaggatgtg ggcgtgacaa acatagagag tgatacggct acgcatcccc
      481 ataacgaact gctgttcgta acggctgaag gtggcatcgt cgccttgttt ggttttttgc
      541 tgctatcagg cgtttggctg tggccgtgta tatgccgcct gaaaatgaca aacaataacg
      601 cgcaccatca atgtgaaaag tggtttattg ccttaatggg gttgccttta gtcattcata
      661 tgatgaccga atatcctctc tatacgtctg ttccccattt gatgctgttg ctgctccttt
      721 tccgcatcgg aatgccggaa aacgtgatga atcaggtacg tatacctaaa acagtccggt
      781 tgggtattct gcctgtcatt gtgctcatta tgaccgccgc gctggtcatc ctggtagcgg
      841 gatttgatgc acagcgggaa ctgaccagag ctgaagctga tatgaacgaa ggatttatcc
      901 ctcgccttcc ggaacatagc tggaaatacc tgacgcaggc agaacgtctt gagcgcgatc
      961 agcatctgct tattgcgaat aaaccggggt tcgttcgcga tacggaaagc atgtctcttt
     1021 tcactgtgtg gggaaataaa tggttggcaa tccacaatga tgcagacgta agcgctgcaa
     1081 tgatattcat tgcgcacagg cgcggtgatt atctgacagc tgaaaaaata agaaaacgcg
     1141 cagctcgggt atttgtaaat gacgaacgtt ttactcgcgg gggaaactaa tggttaacga
     1201 tctgacgtat aagaaattag aaagcacaaa catcaactgt aatattctct cgccagaaaa
     1261 taccacatgg acaaatgtca cggtgattgg cctggatctt tttgccattt gcggcgtgtg
     1321 cagtttgctc aagcgtgttg ggggagtgaa acttcaggtt ttcacttcct tacccactga
     1381 tatgaatgtt ctgactgaca gtagcatcgt gatatgggta aaaatgcgtc acgatggttt
     1441 gcctgaatta gccggacata ttgtgaaggt ttgtcgtcga taccgtctga tgcggcaact
     1501 cgttattagc gatgctattc ctgaaggaat gacgaatgat atcgggcctc tttcccgagt
     1561 atggcttact catgggagtg caaaagtgga tattttatta tcttcattac ggaaggtgtt
     1621 aaatgctcat tgtttgcctg gtcctttgtt tataaaacgc ctgggaagag tgcaatggag
     1681 ggttctttta ctccgcgcga aggggattag cacacgtaaa attgcagaaa tctgtggtat
     1741 tggcctcaag accgttagcg cccatgagtc cgcaattcga gaacgattag gcaccaggaa
     1801 taaaagtgag tacgtctggt tagtacgtaa tgctcaactt atacaacagg cgatacccgc
     1861 gttgggaaga gatgcgtcaa ggtatgaaag atgtaacaat atgcgaacaa agcaaaaagt
     1921 ataattgatg cgtcatagat aactctcttt ttcgatgacg aaatcgctga gcatgcgtgg
     1981 tgaggttagt aatagatggg tgctatgttt tgccacgggt ttgtagctgt gaaacctgga
     2041 aaaaaataag aattaaaaat atttaaagag agttaataag gttctggttg taattgtctt
     2101 ggtcaaaaaa aagatattgt caatgttgtt ttttaaattt tcaggttttg gttaaatatt
     2161 tgcttgcaaa tataggtgat tttttattgt aggcttttga aggctattat aactgtgcgc
     2221 taaattaatg cgttgaataa aagaaataac aaaattaaga aaagccatat ttacgatatg
     2281 ggcaacataa atataatcta aaaaagagta aggcgttgta ggttggttcc gcgttcatta
     2341 ccttgttagt gaacgcggat ttatcagttg tcgaaatggt ttttataata atgaaaaatt
     2401 ttaatgtact aaccaaagtc gtattttgct tgtcgttttt atcaagcgca tttcccacct
     2461 atgctgcgga tgatgtcaaa gtgactcaaa acacttcagt ttttaaactg aagcttagtg
     2521 caacgcgatt aatctatgaa caagggtcgg atggcgcagc gataactgtg acaaatccgc
     2581 aggattggcc gatgcttgtg cagagtaaag tctttgaaga agataaagta actgcagcgc
     2641 cctttattgt cactccgcca ctgttcaggc ttaatccagg ccagcaaaat aaaatgtggg
     2701 ttgtgcagac tgcgccagct ttacaagaca gggaaaaatt acactggcta tgcgccaccg
     2761 gcattacgcc taaaaagagt gatgtttggg ccgagaaaga taaagaaaaa attgctgaaa
     2821 atgtgaacct gaacatccag gttaacgtac atacctgcat aaaacttatt accaggccat
     2881 ctggtattga aggcaagatg gttaatgcga gtgagaagtt atcgtggaaa aagcagggtg
     2941 ataaaattgt cgcggtcaat cagtcgccgt tctacatcaa cctttcttct ttacaggttg
     3001 gcggtagcaa gattgagaaa ctagattata ttgcgccgtt ttcgcaacat gagtttgtta
     3061 tgccaaaaac tagcggccag gatattaaat ggtctgtgat tactgatgaa ggtggtgaga
     3121 gtcgtttgtt tatatctcat ttgcaatcat aatgtattac atttttattg aatattttta
     3181 tgccatacat attccatgtt ttatcgactg tcattaattt tgataatggc cttacttgcg
     3241 ggtcagttaa gtgcccagga gtggtcattt gattcttcac agttggaagg aaacgttagt
     3301 gcggacactg tggcaatgtt taatcaggga gagcaactcc ccggaaatta tcgtgtggaa
     3361 atttatctta acggtgaaaa agttgatgtt ggggagtttc cctttcatcg tccggaatcc
     3421 cctgaagaaa aagagcttgt tccatgttta accgttgatg accttatcca ctatggcatt
     3481 aaaattgata aaagcagcag tgatactgac aataaaaaga atcagtgttt taaatggaac
     3541 tctattgaag gtttgaaagt aaattatgat tttgatagcc agcgcgtaca gataaccgtt
     3601 ccacaactgt atctgcaaga taaaaaaagt agtttagcac cagtgtcgtt atggaatgag
     3661 ggtgtagctg cattccgcat ggtgtatcag acaaatattg atatttccaa acaaaatgat
     3721 aatcaatcta ccacgcgtaa ttctcgttat ggcaggttca ctccagggtt taatcttggt
     3781 gcctggaggt ttcgtagttc agtgacatgg agcaaggaac ttggccagtc agaaagatgg
     3841 caaaggggtt acatgtggtt tgagcgaggc ataaatgcga ttaaaagccg attaacgctt
     3901 ggagaatctt atacgtcgtc tgaggtgttt gacagcatac cttttcgcgg cgggatgtta
     3961 gcgaccgacg atgcgatgac tccgccggag gacagctact acacacctgt ggtgcatgga
     4021 atagcacaaa gtgaggcgca ggttattatt aaacaaaatg ggcaaattat ttttacccgt
     4081 tctgtgccgc cagggccttt tgcgctggat aatttaccaa cgctggccgt tggcggggag
     4141 cttgatgtta cagtgagaga gagtaatggc gaggagcagt atttttccgt gccatttcaa
     4201 acgccggcca tcgctctgca tgaa
//
LOCUS       DAJTYH010000202         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTYH010000202 DAJTYH010000000
VERSION     DAJTYH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(886..1077)
                     /locus_tag="OO609_003979"
     CDS             complement(886..1077)
                     /locus_tag="OO609_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6635183.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO609_003980"
     CDS             1142..1465
                     /locus_tag="OO609_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6635184.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO609_003981"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO609_003981"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO609_003982"
     CDS             2143..2673
                     /locus_tag="OO609_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6635185.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO609_003983"
     CDS             2680..2913
                     /locus_tag="OO609_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6635186.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO609_003984"
     CDS             complement(3459..3851)
                     /locus_tag="OO609_003984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635187.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTYH010000203         3978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTYH010000203 DAJTYH010000000
VERSION     DAJTYH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..662)
                     /locus_tag="OO609_003985"
     CDS             complement(<1..662)
                     /locus_tag="OO609_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR6635188.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QY"
     gene            complement(720..1244)
                     /gene="sat2"
                     /locus_tag="OO609_003986"
     CDS             complement(720..1244)
                     /gene="sat2"
                     /locus_tag="OO609_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6635189.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(1339..1812)
                     /locus_tag="OO609_003987"
     CDS             complement(1339..1812)
                     /locus_tag="OO609_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6635190.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2144..2680
                     /locus_tag="OO609_003988"
     CDS             2144..2680
                     /locus_tag="OO609_003988"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6635191.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2795..3121
                     /locus_tag="OO609_003989"
     CDS             2795..3121
                     /locus_tag="OO609_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6635192.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(3309..3548)
                     /locus_tag="OO609_003990"
     CDS             complement(3309..3548)
                     /locus_tag="OO609_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6635193.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1073 a          908 c          879 g         1118 t
ORIGIN      
        1 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
       61 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
      121 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
      181 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
      241 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
      301 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
      361 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
      421 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
      481 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
      541 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
      601 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
      661 atgatgttta actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt
      721 taggcgtcat cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact
      781 tgaggtctag ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg
      841 tacaaattgc aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt
      901 ctgcttagtg cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg
      961 tgcgtgtgcg acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt
     1021 tcaatcttcc cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca
     1081 gagtcatcat ccgagatgta atccttccgg taggggctca cacttctggt agatagttca
     1141 aagccttggt cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca
     1201 tccaatgttt ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct
     1261 ggcacagcgg atcgcaaacc tggcgcggct tttggtacaa aaggcgtgac aggtttgcga
     1321 atccgttgct gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga
     1381 ggcgaagtct tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc
     1441 accttccggc tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgatcaggct
     1501 tttgtatatc tccccaccac ctgaaacaat gacatgatcc gttattttct ttaggttggt
     1561 taaagcatct ttaattgatg gaaagatcaa tacgttctca ttgtcagatg taaaacttga
     1621 acgtgttacg accgcatact ttcggttggg taatgctccc attgattcaa aagtcttgcg
     1681 tccaaccaac agccattggt tataggtaat agctttaaac aggagctgtt cacctttggc
     1741 actccatgga atatcagggc cattcccgat aactccattc ttcgatatag ctaccattag
     1801 tgatagtttc acaattcttc ctcagaggtt aacattttat tagtaagcat gctcgttttg
     1861 tcacccgctg atgcttaccg ttaattaata taatcacttg tattaaatag actttcttta
     1921 aaaatacaag acactctgtt attacaaatc gtgcatgccg tctatcctgc ttgcacgatg
     1981 cacattatct cacccaaaga actcataaca ttttaattta tttagcatta aattaaaact
     2041 ctaccggtaa acaagcatct ctaggcgtaa aaaacaggca cttttttaaa tcccagctag
     2101 aataggctgt ataggcagac agttgcaaga caaggagcgt tttatgtcta acagtccatt
     2161 tttaaattct atacgcacgg atatgcgaca aaaaggttat gcgctgaaaa ctgaaaaaac
     2221 ttacctgcac tggattaagc gttttattct gtttcacaaa aaacgtcatc ctcagaccat
     2281 gggcagtgaa gaggtcaggc tgtttttatc cagcttagca aacagcagac atgtagccat
     2341 aaacacgcag aaaatcgctt taaatgccct agcttttttg tacaacaggt ttttacaaca
     2401 gccgttgggc gatattgatt atatccctgc aagcaagcct agacggctac cctctgttat
     2461 ctctgcaaat gaagtgcaac gcattttgca ggttatggat actcgcaacc aagttatttt
     2521 tacgctgctg tatggtgcag gtttgcgcat taatgaatgc ttgcgtttgc gggttaaaga
     2581 ttttgatttt gataatggct gcatcactgt gcatgacggt aagggtggga aaagcagaaa
     2641 cagcctactg cccacgcgcc taatcccagc aataaaataa ctcattgagc aagcgcggct
     2701 tattcagcaa gacgacaact tacaaggcgt agggccatcg ctgccttttg ctttagatca
     2761 caaataccct tctgcttatc gacaagcggc gtggatgttt gtctttccct ccagcacgct
     2821 ctgcaaccac ccgtataacg gcaaattatg ccgccatcat ctgcatgact ccgttgcgcg
     2881 aaaggcattg aaggcagccg tacaaaaagc aggcatcgtt agcaagcgtg tcacttgtca
     2941 tacatttcgt cactcgtttg ctacgcatct attacaagcg gggcgtgata ttcgcactgt
     3001 gcaagaactc ttagggcata acgatgttaa gaccacgcaa atctatacgc atgtgttggg
     3061 tcagcatttt gccggcacca ccagtcctgc ggatggactg atgctactta tcaatcagta
     3121 aaagtagata aatatcttta tgtagccctg agtagcccct gatacttttt atggtgaaaa
     3181 agataaacaa tcatagaggg gataaatatg agccagttct accattgttc agataagttt
     3241 aagcaagata ctgtcaatca cagtgaccat tcatggtcat tacacttcct ccagttgcca
     3301 gacaatgcct aacataaaaa cgcaaaagcc caaaagtcag agcttaggtg ttctaggcgt
     3361 tttctaagcg ctgaagaaat acgtcttacc gtagattgcg tgcgtttctg aagcacattt
     3421 tccttataca tggctttatc ccaggcagct tcatcaagct cttggagtaa caagccagta
     3481 ataatgcgac actctcgggt ttgtagcgag ccgctggtga gatcggagtt atagcggaaa
     3541 ggggtcattt gtaattattt gtgggtggag gatagttaac ttattattgt agttggaaga
     3601 ggctgctttc caaacattag cacctcgtaa tgcttgttac tgtatattaa tttatgctct
     3661 tagcgcatgt taatgggcta ttacaatgca taatgtccac atcaagataa ctgtggctta
     3721 acacctgtac aagcgaaaaa aatcaatcac ctgtccggaa atacgggatc actgtaaagt
     3781 gttggtgagg cagtctagga gggggattta atttgggata ctgatgaaag gattatgatc
     3841 agttggatat aggatcgtac taagctgagc ggcaggttag ctagattagt taaccagata
     3901 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     3961 acacccagtt cccatcta
//
LOCUS       DAJTYH010000204         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTYH010000204 DAJTYH010000000
VERSION     DAJTYH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            437..2089
                     /locus_tag="OO609_003991"
     CDS             437..2089
                     /locus_tag="OO609_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6635194.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO609_003992"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO609_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO609_003993"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO609_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO609_003994"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO609_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTYH010000205         3797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTYH010000205 DAJTYH010000000
VERSION     DAJTYH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(69..716)
                     /gene="ychE"
                     /locus_tag="OO609_003995"
     CDS             complement(69..716)
                     /gene="ychE"
                     /locus_tag="OO609_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6635195.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1193..>3797
                     /gene="adhE"
                     /locus_tag="OO609_003996"
                     /gene_synonym="adhC"
     CDS             1193..>3797
                     /gene="adhE"
                     /locus_tag="OO609_003996"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6635196.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYV"
BASE COUNT         1033 a          953 c          890 g          921 t
ORIGIN      
        1 cgcgggaaca aagtaaaaat aatgacgaag taaacggagc atggcagctc cgtttcattt
       61 gaaaggaatt aattaagcag gccggggaaa atccccttaa taccagtaac gataaattca
      121 atccccaatg ccatcagcaa tagccccata atacgcgtaa tcacgttgat gccggtctgg
      181 cgtaaaaccc gtaccagcca cggtgccatg cggaacaatc cccagcaaca taaagcgaac
      241 aatgcaatag ccacaaagaa accaaacaga tagctaatgc tgtgataacg cgtaccccag
      301 acgatggtag aactgatcgc ccctggcccc gccatcaacg gcaacgccaa tggcaccaca
      361 ccaatacttt cacgtaccgc ggtttctgat ttttcttgct tgttctgttt atcctcgcca
      421 agcttgccgc tgatcatcga catcgctatc gtcaccacca ggatgccccc ggcgatacgg
      481 aacgaatcaa ttgatatacc aaaaagttgt agaatcgtgt cgccgagaaa aagcgagatc
      541 cacaagataa tggccacaga caggttggct gtaaggttag ttttgtttcg cgctgccgct
      601 gtctgataac tggtcatgct gataaagacg ggaataatcc ctaccgggtt gaccagcgca
      661 aataacccga tgaaaaattt gaagtaaacg ggaaaatcaa aaagggtctg aatcacggtt
      721 cgctccgaag caaaagccgg ataatgttag ccataaataa ggttgaaaag acgcgctgac
      781 aatacgcctt ttgacagcat ttttcacctc ctaactactt aaaattgcta tcattcgtta
      841 ttgttatcta gttgtgcaaa acatgctaat gtagccacca aatcatacta caatttatta
      901 actgttagct ataatggcga aaagcgatgc tgaaaggtgt cagctttgca aaaatttgat
      961 ttggatcacg taatcagtac ccagaagtga gtaatcttgc ttacgccacc tggaagtgac
     1021 gcattagaga taataactct aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc
     1081 gcgagtaagc ttttgatttt catcggttaa gcaaatcatc accgcactga ctatactctc
     1141 gtattcgagc agatgattta ctaaaaaagt ttaacattat caggagagca ttatggctgt
     1201 tactaatgtc gctgaactta acgcactcgt agagcgtgta aaaaaagccc agcgtgaata
     1261 tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc tggctgctgc
     1321 agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga
     1381 agataaagtg atcaaaaacc actttgcttc tgaatatatc tacaacgcct ataaagatga
     1441 aaaaacctgt ggtgttctgt ctgaagacga cacttttggt accatcacta tcgctgaacc
     1501 aatcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg ctatcttcaa
     1561 atcgctgatc agcctgaaga cccgtaacgc cattatcttc tccccgcacc cgcgtgcaaa
     1621 agatgcaacc aacaaagcgg ctgatatcgt tctgcaggct gctatcgctg ccggtgctcc
     1681 gaaagatctg atcggctgga tcgatcaacc ttctgttgaa ctgtctaacg cactgatgca
     1741 ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta aagccgcata
     1801 cagctccggt aaaccagcta tcggtgtagg cgcgggtaac actccagttg ttattgatga
     1861 aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct tcgacaacgg
     1921 cgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg acgcagtacg
     1981 tgaacgtttc gcaacccacg gcggctatct gttgcagggt aaagagctga aagctgttca
     2041 ggatgttatc ctgaaaaatg gtgcgctgaa cgcggctatc gttggtcagc cagcctataa
     2101 aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga tcggtgaagt
     2161 gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat
     2221 gtaccgcgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg ttgctatggg
     2281 cggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc
     2341 ttacttcggt cagaaaatga aaacagctcg tatcctgatt aacaccccgg cttctcaggg
     2401 tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg gttgtggttc
     2461 ctggggtggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac
     2521 cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta tctacttccg
     2581 ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca aacgtgcgct
     2641 catcgtgact gaccgcttcc tgttcaacaa tggttatgct gatcagatca cttccgtact
     2701 gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagcggacc cgaccctgag
     2761 catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct
     2821 gggtggtggt tccccgatgg acgccgcgaa gatcatgtgg gttatgtacg aacatccgga
     2881 aactcacttc gaagagctgg cgctgcgctt tatggatatc cgtaaacgta tctacaagtt
     2941 cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc accacttctg gtacaggttc
     3001 tgaagtcact ccgtttgcgg ttgtaactga cgacgctact ggtcagaaat acccgctggc
     3061 ggactatgcg ctgaccccgg atatggcgat tgtcgacgcc aacctggtta tggacatgcc
     3121 gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg aagcttatgt
     3181 ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga aactgctgaa
     3241 agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt
     3301 tcacagtgca gcaactatcg cgggtatcgc atttgcgaac gccttcctgg gtgtatgtca
     3361 ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg caaacgccct
     3421 gctgatttgt aacgttattc gctacaatgc gaacgacaac ccgaccaagc agactgcatt
     3481 cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgccg accacctggg
     3541 tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg catggctgga
     3601 aacgctgaaa gctgaactgg gtattccgaa atctatccgt gaagctggcg ttcaggaagc
     3661 agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg
     3721 cgctaacccg cgttacccgc tgatctccga gctgaaacag attctgctgg atacctacta
     3781 cggtcgtgat tatgtag
//
LOCUS       DAJTYH010000206         3788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTYH010000206 DAJTYH010000000
VERSION     DAJTYH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            85..753
                     /locus_tag="OO609_003997"
     CDS             85..753
                     /locus_tag="OO609_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635197.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(996..1691)
                     /locus_tag="OO609_003998"
     CDS             complement(996..1691)
                     /locus_tag="OO609_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6635198.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1684..3111)
                     /locus_tag="OO609_003999"
     CDS             complement(1684..3111)
                     /locus_tag="OO609_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6635199.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3122..>3788)
                     /locus_tag="OO609_004000"
     CDS             complement(3122..>3788)
                     /locus_tag="OO609_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6635200.1"
                     /translation="ARDSVLLLEKLGCRVNFPEKQGCCGQPAINSGYIKEAIPGMKNL
                     IAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAEKVAARMQDLTSFIVNKLG
                     VVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGLELLTFAEQDTCCGFGGTF
                     SVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISGRLQREGQKVKVVHIAEVL
                     MSR"
BASE COUNT          987 a          995 c          808 g          998 t
ORIGIN      
        1 tttgctgata tttaacccga acattcctga tacagactat taatacaaca ttatctgcga
       61 cagattttat tttctggata tattatgagt gaacaaatca aacaggatat cgatctgatt
      121 gagattttat ttcatctgaa gaaaaagatt cgtgttatcc tttttattat ggctatctgt
      181 atggcaatgg tgctgttgtt tctgtatatc aataaagaca atacaaaagt gatttacagc
      241 cttaaaataa accagacaac gccaggtata cttgttagct gtgatagcaa taataatttt
      301 gcctgtcaga ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcagacc
      361 agcccagata tcaagaacag agaaataagg ctggaatggt caggagataa gagagattta
      421 ccaactgctg aagaggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca
      481 gaataccata atggcaggca agttctcgat gagatacaaa cgccttcagc aattaacagt
      541 gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat
      601 ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc catttgcctg
      661 gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg
      721 gtagatgaat accaacaaaa ctctgggcag taataaacag cattctgata gagagtgcct
      781 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
      841 ctttttcact gtaaatatcc tggcattact aatgcctgga tatttacaga agagcagcat
      901 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
      961 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     1021 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgag
     1081 ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg cgctttctga
     1141 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     1201 agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc
     1261 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctct
     1321 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     1381 tgcaaacgtt cactaatccc caattcctcc agcctcgtgt caccgctaat cacgaccgac
     1441 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     1501 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     1561 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     1621 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgctc
     1681 cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac ggaaactctc
     1741 tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag
     1801 tggtgttttg ccgccattga taaaccagct tgccgcatga gcgccggcca tcatcccgac
     1861 tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc gttgctctgc
     1921 ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca gttttgacag
     1981 cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa
     2041 atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa ttggccctgg
     2101 ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag tattcataca
     2161 agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca cctcagaacg
     2221 cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg
     2281 cggccctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag
     2341 catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca ttcccatcac
     2401 tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat tggtcaccag
     2461 gcataccgaa ccggtctctg ccaccgcgaa attacagccg gtaataccta tttcagcact
     2521 gaggaaatct tcacggattt tttgccggat gaataaggtc atcgcttcag gcgtttccgg
     2581 cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat ctttatgaat
     2641 tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat attcacccag
     2701 atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac caatctcttc
     2761 ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg caacctgtaa
     2821 aatgtagcgg gtagcgtctt ctttggtttt tgcaaaatag acgtgaccgc cgttttgcgt
     2881 cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat gatcacgtat
     2941 ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca ttttttgccg
     3001 atttgcccca atacgctgct gcgcgcttgc caccgctttg cgcatgatcg gatcttcaat
     3061 ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca tctttatatc
     3121 ctcagcggct catcaacact tcagcaatat gcaccacttt gactttctgc ccttcccgtt
     3181 gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa tactcagggc
     3241 ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt tcggccattt
     3301 tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag gtcaacagct
     3361 ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc agcttacggg
     3421 ccaggctaca agatgggtga tacacagctc tcccttgcaa actggcacct acatcgacta
     3481 cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca accttttcgg
     3541 cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt atggcatagg
     3601 tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg gcgatcagat
     3661 ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc tgaccgcagc
     3721 atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc agcacggagt
     3781 ctcgtgcc
//
LOCUS       DAJTYH010000207         3534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTYH010000207 DAJTYH010000000
VERSION     DAJTYH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..2387
                     /locus_tag="OO609_004001"
     CDS             <1..2387
                     /locus_tag="OO609_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6635201.1"
                     /translation="DLLKRRDNGKTTWLAWLRQSPAKPNSRHMLEHIERLKAWQALDL
                     PTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYATLVALATEGMATVTDEIIDL
                     HDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIGQALIDAKQSGRDAFAAIEAV
                     MSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRYAPEFLAVLKLRAAPAAKNVL
                     DAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLR
                     SGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAVATDCEQYLHERLTLLEAQLA
                     TVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVD
                     EWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAW
                     HTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGS
                     SPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDH
                     VFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILR
                     LATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVH
                     AGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAA
                     HALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
     gene            2454..2831
                     /locus_tag="OO609_004002"
     CDS             2454..2831
                     /locus_tag="OO609_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6635202.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(3032..3493)
                     /locus_tag="OO609_004003"
     CDS             complement(3032..3493)
                     /locus_tag="OO609_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HCR6635203.1"
                     /translation="MNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYH
                     DDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFA
                     PVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
BASE COUNT          812 a         1084 c          958 g          680 t
ORIGIN      
        1 acgatctgct caagcgccgg gacaacggca agacgacctg gttggcttgg ttgcgccagt
       61 ctccggccaa gccaaattcg cggcatatgc tggaacacat cgaacgcctc aaggcatggc
      121 aggcactcga tctgcctacc ggcatcgagc ggctggttca ccagaaccgc ctgctcaaga
      181 ttgcccgcga gggcggccag atgacacccg ccgacctggc caaattcgag ccgcaacggc
      241 gctacgccac tctcgtggcg ctggccaccg agggcatggc caccgtcacc gacgaaatca
      301 tcgacctgca cgaccgcatc ctgggtaagc tgtttaacgc tgccaagaat aagcatcagc
      361 agcagttcca ggcgtcaggc aaggccatca acgccaaggt acgtctgtac gggcgcatcg
      421 gtcaggcgct gatcgacgcc aagcaatcag gccgcgatgc gtttgccgcc atcgaggccg
      481 tcatgtcctg ggattccttt gccgagagcg tcaccgaggc gcagaagctc gcgcaacccg
      541 atgacttcga tttcctgcat cgcatcggcg agagctacgc caccctgcgc cgctatgcac
      601 cggaattcct tgccgtgctc aagctgcggg ccgcgcccgc cgccaaaaac gtgcttgatg
      661 ccattgaggt gctgcgcggc atgaacaccg acaacgcccg caagctgcca gccgatgcac
      721 cgaccggctt catcaagccg cgctggcaga aactggtgat gaccgacgcc ggcatcgacc
      781 ggcgctacta cgaactgtgc gcgctgtccg agttgaagaa ctccctgcgc tcgggcgaca
      841 tctgggtgca gggttcacgc cagttcaagg acttcgagga ctacctggta ccgcccgaga
      901 agttcaccag cctcaagcag tccagcgaat tgccgctggc cgtggccacc gactgcgaac
      961 aatatctgca tgagcggctg acgctgctgg aagcacaact tgccaccgtc aaccgcatgg
     1021 cggcagccaa cgacctgccg gatgccatca tcaccgagtc gggcttgaag atcacgccgc
     1081 tggatgcggc ggtgcccgac accgcgcagg cgctgataga ccagacagcc atggtcctgc
     1141 cgcacgtcaa gatcaccgaa ctgctgctcg aagtcgatga gtggacgggc ttcacccggc
     1201 acttcacgca cttgaaatcg ggcgatctgg ccaaggacaa gaacctgttg ttgaccacga
     1261 tcctggccga cgcgatcaac ctgggcctga ccaagatggc cgagtcctgc cccggcacga
     1321 cctacgcgaa gctcgcttgg ctgcaagcct ggcatacccg cgacgaaacg tactcgacag
     1381 cgttggctga actggtcaac gctcagtttc ggcatccctt tgccgggcac tggggcgatg
     1441 gcaccacatc atcatcggac ggacagaatt tccgaaccgc tagcaaggca aagagcacgg
     1501 ggcacatcaa cccaaaatat ggcagcagcc caggacggac tttctacacc cacatctccg
     1561 accaatacgc gccattccac accaaggtgg tcaatgtcgg cctgcgcgac tcaacctacg
     1621 tgctcgacgg cctgctgtac cacgaatccg acctgcggat cgaggagcac tacaccgaca
     1681 cggcgggctt caccgatcac gtcttcgccc tgatgcacct cttgggcttc cgcttcgcgc
     1741 cgcgcatccg cgacctgggc gacaccaagc tctacatccc gaagggcgat gccgcctatg
     1801 acgcgctcaa gccgatgatc ggcggcacgc tcaacatcaa gcacgtccgc gcccattggg
     1861 acgaaatcct gcggctggcc acctcgatca agcagggcac ggtgacggcc tcgctgatgc
     1921 tcaggaaact cggcagctac ccgcgccaga acggcttggc cgtcgcgctg cgcgagttgg
     1981 gccgcatcga gcgcacgctg ttcatcctcg actggctgca aagcgtcgag ctacgccgcc
     2041 gcgtgcatgc cgggctgaac aagggcgagg cgcgcaatgc gctggcccgt gccgtgttct
     2101 tcaaccgcct tggtgaaatc cgtgaccgca gtttcgagca gcagcgctac cgggccagcg
     2161 gcctcaacct ggtgacggcg gccatcgtgc tgtggaacac ggtctacctg gagcgtgcgg
     2221 cgcatgcgtt gcgcggcaat ggtcatgccg tcgatgactc gctattgcag tacctgtcgc
     2281 cactcggctg ggagcacatc aacctgaccg gtgattacct atggcgcagc agcgccaaga
     2341 tcggcgcggg gaagttcagg ccgctacggc ctctgcaacc ggcttagcgt gctttatttt
     2401 ccgttttctg agacgacccc tattatggcg aaggtatcat tcaggaagcg ttgatgaagg
     2461 attatctctg taaaaagtta ttcaaccggc tttccggtac cctggtgatc agagcgcgct
     2521 gcggcaataa cattactggc ctggcatgtt gcaatattct ttatccctcg ccccgataca
     2581 gcggtcagct gcatattaaa gagctgtatg tttctcagtg cgatcgaaat aagggcacgg
     2641 gtaaagcgat aatgcgcttt atagcccggc ttgcgcttga acaggaatgc cttagcctta
     2701 gctggaacgc tgaaaaatcc aaccccggcg ctaaccgttt ttatcaggct ctgggaggca
     2761 gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct
     2821 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     2881 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     2941 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caaccaggcg
     3001 tttaagggca ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcagt
     3061 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     3121 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     3181 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     3241 cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     3301 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     3361 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     3421 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cggaattccg
     3481 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgt
//
LOCUS       DAJTYH010000208         3503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTYH010000208 DAJTYH010000000
VERSION     DAJTYH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            65..2062
                     /gene="betT"
                     /locus_tag="OO609_004004"
     CDS             65..2062
                     /gene="betT"
                     /locus_tag="OO609_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR6635204.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2125..3402
                     /locus_tag="OO609_004005"
     CDS             2125..3402
                     /locus_tag="OO609_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR6635205.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          839 a          794 c          886 g          984 t
ORIGIN      
        1 ttgctacttt gtgagaggta gtaccttcta tccagtgcga atttaattaa tggaataaat
       61 gattatgagt gaaaatgata caatcccaaa gaagtctaca agtcagatta acaaagcggt
      121 attctttaca tctgctttgc taattttcct tcttgtcgcc tttgccgccg tattcccgga
      181 tgtcgccgac aaaaatttta aactacttca gcaacaaatc ttcacgaatg ccagctggtt
      241 ctacatcctt gctgtggccc tgattttact gagtgtcacg ttccttggac tctcacgcta
      301 cggtgatatc aagctgggcc cggaccatgc gcagcctgat ttcagctacc actcctggtt
      361 tgcgatgctt ttttcggcag ggatggggat cggcctgatg ttctttggcg ttgccgaacc
      421 tgtaatgcat tatctttcgc cacccgttgg cactccagaa accgttgcgg cagctaagga
      481 agcaatgcgt ctgacctttt tccactgggg actgcacgca tgggcaattt atgccattgt
      541 ggcgctgatt ctggcgttct tcagttaccg tcacggtctg cctttaactc tgcgctccgc
      601 actctatccc attattggcg atcgcatata cggacctgta ggacatgcgg ttgatatttt
      661 cgctgttata ggcacggtct ttggcgttgc gacatcactg ggttacggtg ttttgcaggt
      721 gaatgccggt ttgaaccatc ttttcggggt gcccatcaat gaaacggtgc aggtaattct
      781 gatcgtggtc atcacggggt tagcgacgat ttcagtggtg tccggtctgg ataagggaat
      841 acgtatcctg tctgaactca atctgggtct ggctttgttg ctcctggcgc tggtcctgtg
      901 tctgggacca accgtgcttc tgctgaagtc atttgtggaa aatacgggcg gttatctttc
      961 ggaactggtg agtaaaacgt tcaaccttta cgcgtatgag cccaagtcga gcaactggct
     1021 ggggggctgg acattactgt actggggatg gtggctttca tggtcgccgt ttgtggggat
     1081 gttcatcgca cgggtctccc gcgggcgaac cattcgcgag tttgtcaccg gcgtgctgtt
     1141 tgttcccgcg ggttttacgc taatgtggat gacggtgttt ggtaacagcg cgatctatct
     1201 cattatgaac cagggggcca cagacctcgc caatactgtt cagcaggatg tggcgctggc
     1261 cctgtttaat ttcctggagc atttcccgtt ctcttctgtg ctgtcattca ttgcaatggc
     1321 gatggtcatc gtcttctttg taacgtctgc tgattcgggg gcaatggttg tggatactct
     1381 ggcatcaggt ggagtggcaa acacacccgt ctggcagcga atattctggg cctcgctcat
     1441 gggcattgtt gcaattgcgc ttctccttgc cggagggcta agtgcgctgc aaacggtgac
     1501 aatagcgagt gcattgccct tctcagtgat cttactaata tctatatacg gacttttaaa
     1561 agctttgcgc cgggatttga ccaagcgtga aagcctgagc atggcgacaa ttgctcctac
     1621 ggctgcacgt aacccaattc cttggcagag aaggttacgc aatatcgcgt atctgccgaa
     1681 gcgatctctt gtgaaacgtt ttatggacga cgttatccag cctgccatga cgctggttca
     1741 ggaggaactg aacaagcagg ggacgataag ccacattagt gatgcagtcg acgatcgtat
     1801 tcgtcttgaa gtcgatttgg gcaacgagct gaattttata tatgaagtga ggcttcgcgg
     1861 gtatatctca ccgaccttcg cgctcgccgc aatggataat gatgagcagc agactgaaca
     1921 acatcgatat tatcgcgctg aggtttatct caaagaaggc ggtcaaaatt atgatgtgat
     1981 gggctggaac caggaacagc tgattaatga catactggac cagtacgaaa aacacctgca
     2041 cttcctgcac ctggttcgtt aatagcaaca tgccgtcctg ggggcggcaa ttattatccc
     2101 ggccgcaata tgagggaatg cagaatgatt tcacgctgga aatggatgct gaagcagaca
     2161 attaaaaaac tatggttcag ggcaacgtta ttcgcaattg tcgcgataat aacggccctt
     2221 ttatcaattc tttttaaatc aatgatacct gagtcggttt ccgtgaaggt tggtgcggaa
     2281 gcagtcgata acattctgaa catactggca tcgagtatgc tggcagtgac cacattttcg
     2341 ctgagtatca tggtcacagc ctacggttca gccactacta atgtgactcc cagagctacg
     2401 cgtttagttg ttgaagacgt caccacacaa aatgtactgg ccaccttcat cggttctttt
     2461 ctcttcagtc tggtagggat tattgccctc aatatgggag cttatggaga aagggggaga
     2521 gtcattttat tcattgtcac actggttgtc attgccttaa tcctcatcac attgcttcgc
     2581 tggatacagc atttgacctc tctggggagg gttggtgaga caacggcaca agtagaacag
     2641 gcggccatcg aaacatttat tgcgagagca agaaatccct gtctcggtgg atatccatgg
     2701 cttgagaaca atgaacagcc gaaaggaacg gttgcagttt atccgaagaa gattggctat
     2761 gttgaatata ttgatatggt gaaacttagc aagctgctga ccaatgatcc ccgtcatgta
     2821 tacctcgtgg cgcagccagg cagtttcata catccgtcca tgccagtttt gtacctgagt
     2881 cagggccagg agtcgtcaat caacaccgat ttacttgaga cgattattgt ctcggatgca
     2941 cgttcatttg ctcaggatcc tcgattttgt cttagcgtca tggccgaaat agcctgccgg
     3001 gccctttccc ctgcagtgaa cgatcctgga accgccattg atgtcattgg cagaggtgtt
     3061 cgtatacttt ccacttacgc gcagaataaa tctgatgaaa tagaagtgaa atatccttca
     3121 gtacatgttg caccacttca gaataacgat ctactggaag actttttctc acctgtcgcg
     3181 cgcgatggtg ccagtatgag ggagattcag ataagagtcc ttaaaggact gtcgatgctg
     3241 agtaaaggtt ggcctgagat atttgctgaa gccgcacata ccctggcatt tgaaacatta
     3301 gagcatgcaa ctcgtgctga ccatatagat tctgatagat atctaataaa atcaacttat
     3361 tataatttat ttagtggcga atattctaat aaaaaaacat agctgcagag caagcaattc
     3421 ggttgttggc aagtcaaggg caaagcattc gcgtgagaat gggcgtatgc tttgtgccaa
     3481 aagcaaacgt tgctaattct gca
//
LOCUS       DAJTYH010000209         3495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTYH010000209 DAJTYH010000000
VERSION     DAJTYH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..753
                     /locus_tag="OO609_004006"
     CDS             <1..753
                     /locus_tag="OO609_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6635206.1"
                     /translation="EGRGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDL
                     TMELSSIKAEELTDNAGIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENG
                     TSLVMTGSCGTGKNHLAVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADE
                     VIERYASMDLLIIDEVGVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFI
                     SPRIADRITDGGRNWLSFNWPSYRSRIRGVAA"
     gene            750..2141
                     /locus_tag="OO609_004007"
     CDS             750..2141
                     /locus_tag="OO609_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6635207.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2138..2395
                     /locus_tag="OO609_004008"
     CDS             2138..2395
                     /locus_tag="OO609_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635208.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2490..2690
                     /locus_tag="OO609_004009"
     CDS             2490..2690
                     /locus_tag="OO609_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6635209.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2683..3063
                     /locus_tag="OO609_004010"
     CDS             2683..3063
                     /locus_tag="OO609_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6635210.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            3263..3339
                     /locus_tag="OO609_004011"
     tRNA            3263..3339
                     /locus_tag="OO609_004011"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3297..3299,aa:Ile,seq:cat)
     gene            3341..3417
                     /locus_tag="OO609_004012"
     tRNA            3341..3417
                     /locus_tag="OO609_004012"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3375..3377,aa:Arg,seq:tct)
     gene            3420..3495
                     /locus_tag="OO609_004013"
     tRNA            3420..3495
                     /locus_tag="OO609_004013"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3453..3455,aa:Thr,seq:tgt)
BASE COUNT          909 a          773 c         1009 g          804 t
ORIGIN      
        1 gaaggtcgcg gatattgcga aaaacatggc gaatatcaca cgcatatact ggtgtggagc
       61 gatcgtaatg gcgaggacag agaagaaatt tcatgctgcc ctgactgctt aatcgctgag
      121 gccaacgatt tgaccatgga gctgtcgtcc atcaaggcgg aagagctgac tgataacgcc
      181 ggaattgccc tgcgttttcg ggactgcgag tttgataatt atctggaggt taatcctggc
      241 gcagccagaa atcttgcggc ctgtcgccgc tatgcggaga actggccaga tatgctggag
      301 aacggtacca gtcttgttat gaccggcagt tgtggtaccg gaaaaaatca tctggctgtg
      361 gctatggcaa aacacatcat ccgtaactat ctggccagtg tggagatcac cgacgtgatg
      421 cgccttaccc gtgctgtgaa aaattgctgg cggaatgaca gcgaaaaaac agcggatgaa
      481 gttattgagc gttatgcgtc aatggatttg ctgatcatcg acgaagttgg cgttcagttt
      541 ggcagcgcgg ctgaaatggc tattttgcag gaaattatca acgccaggta cgaaagtatc
      601 ttgcccacca ttctgatcag taacctctca ccggaagagt tgtgggcgtt catcagtccc
      661 cgaattgccg acaggatcac agacggggga cgcaactggt tgtcgtttaa ctggcccagt
      721 taccgttctc gtatcagagg tgtggctgca tgacaactcc agtatggcgt aacgatgacc
      781 ttgaaggcgc tgtcatcggc gcgttctttc tgcgcggagc agatcctgaa gtgatggata
      841 ttctggccac actgccagcg gacgtttttt ctgtacgagc gtatcaggat atctatacag
      901 gcatctgcag acaggcccgt gtttcaggag tgattgaccc cgtgctgttg tgtaatgaga
      961 tgccggaact tgccccggtg attactgata ccgggcgtaa aacctgggtg aagtcttcac
     1021 tggagcacta tgttgcagcg ttgcggcgca atgccgcact gcgcgatgca gaaaaaacac
     1081 tgaatgaggc gctgcagaaa ttacgtgatg cgcatacctg tgaagcagct gaagatgccc
     1141 tgaaggatgc gcagaacatg atggtcacac tgtcgacagg aaagggcgtc attcagccgg
     1201 tacatattga tgatgtgctt ccggaagtgg ttgagcgtgt tgaatgccgg aatcaggggc
     1261 tggagaaatc caggacgttg atgaccggta ttgatgaact ggacgcaaaa acaggcggta
     1321 tggagcccgg agacctggta ttcattgccg cccgtccttc gatggggaaa accgaacttg
     1381 cgctggacat catcgacaag gtgactgagc aggggcatgg tgtgcttctg ttcaccatgg
     1441 agatggcgaa catccagatt ggtgaacgta tggtgtctgc ggctggaggg atgccagtat
     1501 cacgcctgaa atctgtggct cactttgaag atgaagactg gacgcgtttc tcacaagggg
     1561 tggggcggat gaccgggcgc aatatctgga tggtggacca ggcgaacctg gccattgatg
     1621 agatatgcgc aacaacgaaa caccacctga ttaaatatcc ggaaacggcg ctggtggtgg
     1681 ttgattatct cgggctgata aaaacccgaa ccacggggcg tcatgacctt gccgtgggtg
     1741 aaatctcaaa ggggcttaaa ggcctggcaa aatccggtgg ttttccgttg attgcgctga
     1801 gccagctctc ccgcggtgtg gagtccagac ccaataaacg tcccatgaac tcagacctga
     1861 aaaattccgg agaaatagag gcggatgcag acatcattct gatgctttac agggatgaag
     1921 tgtacaaccc ggatacgcag gccaggggca tcgcagaaat caatatcacg aaacaacgta
     1981 acggttctct gggaacgatt taccggcgtt tttataacgg acattttctg cctgtggacc
     2041 aggaaagtgc acgggtgctt tccactccca tgaagcccgg caatccgcgc agatacagta
     2101 ataaacgaac tgacagcagt aagatggagc gtttcttttg aacaaccaga caatgacttt
     2161 tacccctgaa caattgcgta aacacgcgca ggaaatgctg cgtcatgctg aacaactcga
     2221 aaaaacgggc ataacaaaag atgctatccg taaagatatg gtacccgcgc ttcgggaact
     2281 gatgcaggcg aagcatcgcg cacagaaagc agtagatgag ctggtggact gtgtggcaga
     2341 gctggaaacc agagtcggaa agtttgaaaa actggtgcag gaggcgctgc gctgatgcgc
     2401 catgagtttg ttttgccctg gcttgtgcgc gatcattatg tccctggtgg aaagcccggg
     2461 ataaaaatca ccgaaccgga gagtatttga tgagcgtcaa aattcaaacg ataccggaac
     2521 tgcttatcca gacacgaggt aatatgaccg aagtttcacg aatgctgaac tgtaatcgcg
     2581 ccacggtaag aaaatatgct gaagataaag agggcaaagg gcacgccatt gttgatggtg
     2641 ttctgattgt tcatcgtgga tgggacaggg gtaaagacag tgatgcgtga tattcagcag
     2701 gttcttgagc gctggggcgc gtgggtggct aataatcatg aggatgtgac ctggtcgtcc
     2761 attgctgccg gttttaaggg attaatccct tcaaaagtaa aatcccgccc gcaatgctgt
     2821 gacgatgacg cgatggttat atgtgggtgt atggcccgcc ttaaccggaa caacaacgat
     2881 ctacatgact tgctggttga ctactacgtg tttgggatga cattcatgac gctggcgcgg
     2941 aagcatggat gctcagatgg ttatatagga aagaagctac aaaaagccga gggggtggtg
     3001 gaaggaatgt tgatgatgtt gggtgtccgg ttagaaatgg acaaatatat tgaacatccc
     3061 tgatggtaag gggcgacttt ctagaaaata ttttacgtac gtaaaaaacg ggatattctg
     3121 ttaagagtgg ttgcttcgcc acacagctta aacccgcctc cgggcgggtt ttttatgcct
     3181 gaaaaacggt acaggacgtt aaacacgctg gtggtcagat gagtttgcag atgtgatgac
     3241 atatggttat tattctgcct ccggcccttt agctcagttg gtcagagcga gcgactcata
     3301 atcgccaggt cgctggttca agtccagcaa gggccaccaa ccaccactag ctcatccgga
     3361 tagagcatca accttctaag ttgacggtgc gaggttcgag tcctcggtgg tgggccagcg
     3421 ccgacttagc tcagcaggca gagcaactga cttgtaatca gtaggtcacc agttcgattc
     3481 cggtagtcgg cacca
//
LOCUS       DAJTYH010000210         3488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTYH010000210 DAJTYH010000000
VERSION     DAJTYH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..389
                     /locus_tag="OO609_004014"
     CDS             <1..389
                     /locus_tag="OO609_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6635211.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO609_004015"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO609_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6635212.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO609_004016"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO609_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6635213.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO609_004017"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO609_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6635214.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3116..3487
                     /locus_tag="OO609_004018"
     CDS             3116..3487
                     /locus_tag="OO609_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6635215.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          948 a          641 c          716 g         1183 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgag
//
LOCUS       DAJTYH010000211         3469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTYH010000211 DAJTYH010000000
VERSION     DAJTYH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(129..2162)
                     /gene="metG"
                     /locus_tag="OO609_004019"
     CDS             complement(129..2162)
                     /gene="metG"
                     /locus_tag="OO609_004019"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6635216.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2294..3403
                     /gene="apbC"
                     /locus_tag="OO609_004020"
     CDS             2294..3403
                     /gene="apbC"
                     /locus_tag="OO609_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6635217.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          839 a          874 c          926 g          830 t
ORIGIN      
        1 taattttatc cagttgaatg cagatgctac cagtatttat acgggttaga gagagacaaa
       61 atcgcaagaa aacaacttta ggaatttata aagaaaaggc gctgtcgatg cagcgccttg
      121 aagggggatt atttcacctg atgacccggt ttagcaccgg catccgggct tagcaggaaa
      181 atatctttcc cgccaggacc ggcagccatc accatgcctt cagagatacc gaagcgcatt
      241 ttacgtggtg ccaggttagc caccataatg gtgtgacgac caatcagtgc ctgcggatcc
      301 gggtaagcag aacgaatacc ggagaagaca ttgcgttttt caccgccgag atccagcgtc
      361 aggcgcagca gtttgtcaga accttcaaca aactctgcgt tttcgatcag cgccacgcgc
      421 aggtcaactt tggcgaagtc gtcaaaggtg atggtttcct gaatcggatc atctgccagt
      481 gggccagtaa ctggcgcggt agtggctttc acttcttctt tagaggcttc caccagagct
      541 tcaacctgtt tcatatcgat gcggttatac agcgccttga acggattcac tttgtggccc
      601 agcagcggtt gctggatacc atcccaggtc agttccgtat tgaggaaggc ttctgcacgc
      661 tcggtcagtt tcggcagtac aggcttcagg taagtcatca gtacgcggaa caggttgatg
      721 cccattgagc aaatcgcctg caggtcagca tcgcgacctt cctgttttgc gactacccac
      781 ggagcctgct cgtcgacata gcggttagcc aggtcagcca gtgccatgat ttcgcgcact
      841 gctttaccaa attcacggct ttcccacgct tcaccaatca cttcagcggc atcggtaaag
      901 gttttgtaca gctcagggtc agccagctcg cttgccagca caccgtcaaa acgcttgttg
      961 ataaagcccg cattacggga ggccaggtta accactttgt tcacgatatc ggcattcaca
     1021 cgctgaacga aatcttccag gttgagatcg atatcatcaa tgcgcgaaga gagtttcgca
     1081 gtgtagtagt aacgcaggct gtcagcgtca aaatgattca gccaggtgct ggctttaata
     1141 aaggtgccgc gagacttgga catcttcgcg ccgttcaccg tcacatagcc atgaacaaac
     1201 aggttggtcg gcttgcggaa gttgctgcct tccagcatgg caggccagaa caggctgtgg
     1261 aagtaaacga tatctttacc gatgaagtgg tacagctcgg cggtggagtc tttcttccag
     1321 tattcatcga agcttacgct gtcgccgcgc ttgtcgcaca gattcttgaa agaacccatg
     1381 tagccaatcg gtgcgtccag ccagacgtag aaatatttgc ccggtgcgtt cggaatttca
     1441 aaaccaaagt atggcgcgtc gcgggagata tcccactgtt gcaggccgga ttcaaaccac
     1501 tcctgcattt tgtttgccac ctgctcctgc aacgcaccgc tgcgggtcca tgcctgcagc
     1561 atttcgctga aagagggcag atcaaagaag aagtgttcag aatcacgcat cactggtgta
     1621 gcaccagaaa ccaccgattt cggctcgatc aattcagtcg ggctgtaggt cgcgccgcag
     1681 acttcgcagt tatcgccgta ttgatccggg gatttacatt tcgggcaggt gcctttcaca
     1741 aaacggtccg gcaggaacat gcctttttcc ggatcgtaca gctgagagat ggtgcggttt
     1801 ttaataaaac cgttttcttt caggcgagag tagataagtt ctgacaactg gcggttctct
     1861 tcgctgtgcg tcgagtgata gttgtcatag ctgatgttaa agcctgcgaa atcagtctga
     1921 tgctcctgac tcatttcgcc aatcatctgc tccggggtga taccgagctg ctgagctttc
     1981 agcatgatcg gtgtaccgtg ggcatcgtcg gcgcagatga agttgacctc gtggccgcgc
     2041 attcgctggt aacggaccca gacatcagcc tggatgtgct ccagcatatg gccgaggtgg
     2101 attgagccgt tagcgtacgg cagtgcgcac gtcaccagaa ttttcttcgc gacttgagtc
     2161 atagtaggca ttacttctta atagtgaaaa ggggttagat agtaccaaat gggaaaatgt
     2221 taagtaagta cttctctata ttcttcaggt aaactatcag ctgatggttt atctatacaa
     2281 aaaaggagtc gggatgaacg aacaatccca ggccaaatca ccagaagcct tgcgcgcaat
     2341 ggtcgccggg acactggcca attttcagca cccaaccctg aagcataacc tcaccacgct
     2401 gaaagcgttg caccatgtcg cctggatgga cgacacgttg catgtggaac tggttatgcc
     2461 gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa tgtagcgccg aactgctgcg
     2521 catcaccggg gcgaaagcta tcgactggaa actgtcgcat aacatagcca ctctgaaacg
     2581 cgtgaagaat cagccgggaa ttaacggtgt gaagaacatt atcgcggtga gttccggtaa
     2641 aggcggcgtg ggtaaatcgt ccacggcggt aaacctggcg ctggcactgg cggcggaagg
     2701 ggcgaaagtc ggcattctgg acgccgatat ctacggccca tcaattccca ccatgctggg
     2761 cgcagaaaat cagcgtccaa cttcgccgga cggtacccac atggcaccga ttatgtctca
     2821 tggcctggca accaattcaa ttggttatct ggtcactgac gacaatgcga tggtgtggcg
     2881 tggaccgatg gcgggcaagg cgttaatgca gatgttgcag gaaaccttgt ggccggatct
     2941 cgactatctg gtgcttgata tgccgccagg caccggtgac attcaattga cgctggcgca
     3001 aaacattcca gtaactggtg cagtcgtggt caccaccccg caggacatcg cgttgatcga
     3061 tgcgaaaaaa ggcattgtga tgttcgagaa agtcgaggtt ccggtgctcg gtattgtcga
     3121 gaacatgagt gtgcatattt gcagtaactg tggtcaccat gaaccgattt tcggtaccgg
     3181 tggtgcagag aaactggctg aaaaatacca tacacagctg ctggggcaga tgccgttaca
     3241 catctcctta cgtgaggatc tcgataaagg cacgccaacg gttatcagcc gtccggagag
     3301 cgaatttacc gctatctacc gccagctggc tgaccgcgtt gcagctcagc tctactggca
     3361 gggtgaagtc attcctggcg agatttcctt ccgcgcggtc taacgcctat taaccattct
     3421 ggttattctt catacaccgc agagatgtta tattgatgcg gtgtatttg
//
LOCUS       DAJTYH010000212         3248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTYH010000212 DAJTYH010000000
VERSION     DAJTYH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..442
                     /locus_tag="OO609_004021"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO609_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO609_004022"
     CDS             1026..1742
                     /locus_tag="OO609_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635218.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO609_004023"
     CDS             2294..2698
                     /locus_tag="OO609_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635219.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3248
                     /locus_tag="OO609_004024"
                     /pseudo
     CDS             2761..3248
                     /locus_tag="OO609_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          670 c          745 g          924 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaa
//
LOCUS       DAJTYH010000213         3182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTYH010000213 DAJTYH010000000
VERSION     DAJTYH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO609_004025"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO609_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6635220.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO609_004026"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO609_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          777 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggggga ccctgagcag gtggtaattg taagacgttg
     2821 cgggcgacgg tattacaatt gcacatcctg tcctgtttct ttaggacgta tcatggcaca
     2881 gcatatcaac tgataaataa tccctgtgca gtgcagccgc tgttgcataa aatttcgctc
     2941 tgcgtcgagt gtttcttaat caataattcc gctgatactc cggaaaaacg cgagtctgtt
     3001 ctcatgcctg aaatgcccac accccacgca aaaacaagtt tttgctgatt tttctttata
     3061 aatagagagt tatgacaaat tagttcttct tgctctcttt gtgatattta aaaaagcggt
     3121 gtcggcgcgg tgttgtagct gcgccaacac cgctttttag gggtggtact gactattttc
     3181 at
//
LOCUS       DAJTYH010000214         3102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTYH010000214 DAJTYH010000000
VERSION     DAJTYH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..699)
                     /gene="ipaH9.8"
                     /locus_tag="OO609_004027"
     CDS             complement(<1..699)
                     /gene="ipaH9.8"
                     /locus_tag="OO609_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6635221.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDY"
     gene            complement(961..1056)
                     /locus_tag="OO609_004028"
                     /pseudo
     CDS             complement(961..1056)
                     /locus_tag="OO609_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1092..1397)
                     /gene="ccdB"
                     /locus_tag="OO609_004029"
     CDS             complement(1092..1397)
                     /gene="ccdB"
                     /locus_tag="OO609_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6635222.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1399..1617)
                     /gene="ccdA"
                     /locus_tag="OO609_004030"
     CDS             complement(1399..1617)
                     /gene="ccdA"
                     /locus_tag="OO609_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6635223.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2153..3102)
                     /locus_tag="OO609_004031"
                     /pseudo
     CDS             complement(2153..3102)
                     /locus_tag="OO609_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          751 c          751 g          827 t
ORIGIN      
        1 gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg ataatgggtt
       61 atcactaata tgtattgaac attcattcct cagattaaga atgctttccg ggatatgact
      121 tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct cagaaaatgc
      181 tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct gtgggagcga
      241 tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat cgggtaaagc
      301 tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag gaggtagcac
      361 gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa caggcagttc
      421 aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag ctggtaagtt
      481 gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat tattgataag
      541 acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg cttgtttttc
      601 ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt tataaaaaga
      661 attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc agttaaagcc
      721 ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt tcattccatg
      781 aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct gtaaaaaaac
      841 gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt gtttttcggt
      901 tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc gtattgatat
      961 ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt ttgccggcca
     1021 gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga tttttgaccc
     1081 tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg
     1141 cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact ggccatatca
     1201 gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag ctcacgggag
     1261 actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg tccgggcgtg
     1321 tcaataatgt cactctgtac atccacaaac agacgataac gactctctct tttataggcg
     1381 taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc cgttcatttc
     1441 aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc gttccgcacg
     1501 cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat tcacgtttgc
     1561 ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct gcttcatgat
     1621 aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt acaaaccatc
     1681 aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat tttttctgct
     1741 gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt acatgaaagc
     1801 aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt aatacggttg
     1861 cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga atggtaccgg
     1921 aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta ctgacacggg
     1981 tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat tcaggttctg
     2041 attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca ggcgttcaac
     2101 aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc actcacccgg
     2161 tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc cattccggca
     2221 gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt tttgctgttt
     2281 ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg gcgaacagcc
     2341 acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg ttgtccaggg
     2401 gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga atatagttaa
     2461 tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca agccaggcaa
     2521 acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt ttttccacag
     2581 ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc agctccacgc
     2641 ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct gcgtgactcc
     2701 agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac acgctgtatc
     2761 cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg tatctgaggg
     2821 cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca ccgctgacgt
     2881 aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg agcctcaggg
     2941 acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc agtttcgcca
     3001 gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg acggggagct
     3061 ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTYH010000215         3089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTYH010000215 DAJTYH010000000
VERSION     DAJTYH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(96..1805)
                     /locus_tag="OO609_004032"
     CDS             complement(96..1805)
                     /locus_tag="OO609_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6635224.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2237..2956)
                     /locus_tag="OO609_004033"
     CDS             complement(2237..2956)
                     /locus_tag="OO609_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6635225.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          951 a          586 c          517 g         1035 t
ORIGIN      
        1 aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga gtgaacatat
       61 caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt aaatctattt
      121 attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat agtatccggt
      181 tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt gtctctattt
      241 actacagata caaatacacc aggaatacca tctttattag ttgcggacag catttcgacc
      301 aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg tattgtttcg
      361 gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt ttttatccat
      421 aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc tccatacgcc
      481 ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt tactttcaat
      541 aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc gaacatacga
      601 atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc atggctgtct
      661 ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa gactatttcc
      721 ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa acccggcaca
      781 ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag attgcacata
      841 tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag tgctatataa
      901 aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt agattgagaa
      961 atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag attctttgtt
     1021 atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt cactgtgttt
     1081 gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc cagaccataa
     1141 attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag ttcgaaatgg
     1201 ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc gaaaaactga
     1261 acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc catttggtgg
     1321 tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat gttataaaaa
     1381 aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata agctggtgat
     1441 tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc aggggtgcta
     1501 agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca ataggaagct
     1561 aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt tatatttacc
     1621 tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg aattagttca
     1681 ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct gttaacagac
     1741 atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt tttatttaac
     1801 ggcatagaag acaaccatag agatgttata atactattag attgcagtta ctaatagtaa
     1861 aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc gagctcgtca
     1921 acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag gcactggaaa
     1981 agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa tatcccgaaa
     2041 agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact gttcggattt
     2101 cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg aatcaggctg
     2161 catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct acaaggtggt
     2221 gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct cgtaacattt
     2281 gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag ctgacatatt
     2341 tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt ttgcttctta
     2401 atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat aaagcactat
     2461 attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct atccccacac
     2521 gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata ggactgtcct
     2581 ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc ctagctatac
     2641 taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca cgacatgcat
     2701 gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg acagaaaaag
     2761 attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag tacgctgggt
     2821 attgccgtat ttggttgtac aatatattga agttattttt caatctttca ttcgcagaaa
     2881 gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt tttagacagg
     2941 gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct tatattgatt
     3001 gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt aagggtcctc
     3061 tccgaacatg gtgggaaggc ttagaacgc
//
LOCUS       DAJTYH010000216         3059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTYH010000216 DAJTYH010000000
VERSION     DAJTYH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..488
                     /locus_tag="OO609_004034"
     CDS             <1..488
                     /locus_tag="OO609_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6635226.1"
                     /translation="LKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVN
                     CPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRIDDAES
                     GNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVTLDY
                     R"
     gene            568..1263
                     /locus_tag="OO609_004035"
     CDS             568..1263
                     /locus_tag="OO609_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6635227.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1292..>3059
                     /locus_tag="OO609_004036"
     CDS             1292..>3059
                     /locus_tag="OO609_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6635228.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          885 a          717 c          679 g          778 t
ORIGIN      
        1 ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt gctcttggtc
       61 actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt ccattcacta
      121 ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac gttgaagcgt
      181 ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc cctaatctgg
      241 caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt attgacgatg
      301 cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg atttatcaga
      361 tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac gtatcaaccg
      421 ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta acgctggatt
      481 accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc cttcctcttt
      541 ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt gatattaacc
      601 ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt acagcgaacg
      661 cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa caaaggcgca
      721 gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag ccgtgcccca
      781 tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc actgcgaatt
      841 attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat taatgttcgt
      901 gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac gctggtattt
      961 aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt aaacgatgcg
     1021 tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa cccaactgaa
     1081 tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag caaaattgaa
     1141 tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg aaagaacgtg
     1201 aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg tccactgcaa
     1261 taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc agggacataa
     1321 acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg tgctcactgg
     1381 cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc ccattatgct
     1441 ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa aaaaatacgc
     1501 acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg tttcacagaa
     1561 aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta cggtagaaca
     1621 actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa aagatgacga
     1681 tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat ttgatttcaa
     1741 tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg caagaggtta
     1801 cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact actcgtttac
     1861 aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc tgaatatgca
     1921 aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga cacgcaacga
     1981 tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca aggcgttgaa
     2041 gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca gctacgcctt
     2101 tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc gcggatttgc
     2161 cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc aaaatggtta
     2221 tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc tctacccctc
     2281 ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac aacgtcgctt
     2341 tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa aatatagcgc
     2401 gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg aatttgctga
     2461 agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc tgctcggttc
     2521 tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt
     2581 gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt ttcatggcta
     2641 tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata tcgctgttag
     2701 ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata cccgcaattg
     2761 gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga caatatttga
     2821 tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata acgagaaaaa
     2881 cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca gcctgaatta
     2941 tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga atctcagtat
     3001 tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat cacttaacc
//
LOCUS       DAJTYH010000217         3020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTYH010000217 DAJTYH010000000
VERSION     DAJTYH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..154
                     /locus_tag="OO609_004037"
     CDS             <1..154
                     /locus_tag="OO609_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6635229.1"
                     /translation="PLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKN
                     LYASMA"
     gene            311..1060
                     /locus_tag="OO609_004038"
     CDS             311..1060
                     /locus_tag="OO609_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6635230.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1082..1648
                     /gene="yieF"
                     /locus_tag="OO609_004039"
                     /gene_synonym="chrR"
     CDS             1082..1648
                     /gene="yieF"
                     /locus_tag="OO609_004039"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6635231.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1702..3000)
                     /gene="adeP"
                     /locus_tag="OO609_004040"
     CDS             complement(1702..3000)
                     /gene="adeP"
                     /locus_tag="OO609_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6635232.1"
                     /translation="MLERVFKLREHGTTARTEVIAGFTTFLTMVYIVFVNPQILGVAG
                     MDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVVVQAMGLPWQIGMG
                     AIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMGLKNAGVIVANSET
                     LVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLGWMLGDVHYNGIVS
                     APPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVTDKAGLADEKGKFP
                     RMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAVVVGLLFLLVIFLS
                     PLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAVMMPFSFSITEGIA
                     LGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT          834 a          801 c          746 g          639 t
ORIGIN      
        1 gccgttggtt gctcttcctc tcccgtttga cgaaagccag caaaaaaagc tggaagttcc
       61 ttttaccctg ctgtggcata aacggaacag ccataatccg aagatcgtct ggttacggga
      121 aaccattaaa aacctttacg cgtcgatggc ataaccgaat cgtatgaaac gcgacccaat
      181 ttcacaataa aatgtaaaaa agttgtaata agcttgtctg aatcgaactt ttagccgctt
      241 tagtctgtcc atcattccag taaatgatta ctcttgtatt cataatggac cattaagcat
      301 ggagcgaaaa atggcgactc actttgcccg agggatttta acggaaggac atctgatttc
      361 tgttcgtctc ccctcccagt gtcatcaaga agcccgaaac attccccctc atcgtcaaag
      421 ccgttttctg gcgtccagag gtttactcgc agaactgatg ttcatgctgt atggcattgg
      481 cgaattgccg gaaatcgtca ccctgccgaa aggtaaaccg gttttcagtg ataaaaattt
      541 gccttcgttt tctatttcct atgccgggaa tatggttggc gtggcgttaa caaccgaagg
      601 tgaatgtggc ctcgatatgg aactacagcg tgcgacgcgc gggtttcata gcccacacgc
      661 gcccgataac cacacctttt ccagcaatga atcgctatgg atcagtaaac aaaacgatcc
      721 taacgaagcg cgggcgcagc tcattacgct gcgccgaagc gtgctaaaac taaccggtga
      781 tgttttgaat gacgatccgc gcgatctgca gctgctgccc attgccggac gcctgaaatg
      841 tgctcatgta aatcatgtag aagcgttatg cgacgcggag gacgtgctgg tgtggtccgt
      901 ggcggtcacg cccgcgattg aaaagctcag tgtctgggag ttagatggca aacacagctg
      961 gaaaagcctg ccggatattc acagccgcgc caacaatcct accagccgga tgatgcgttt
     1021 tgcccagctc tctaccgtga agtctttttc gccaaattga tagacaacca caggagtcat
     1081 tatgtctgaa aaattgcagg tggttacgtt actggggagc ctgcgcaaag gctcatttaa
     1141 tggcatggtt gcacgtaccc tgccgaaaat tgctccggcg agcatggaag tcaatgcgtt
     1201 accttccatt gccgacattc ccctgtatga cgctgacgta cagcaggaag aaggttttcc
     1261 agcaacggtt gaagctctgg cggaacagat ccgtcaggct gacggtgtgg tgatcgtcac
     1321 gccggaatat aactactcgg taccgggtgg gctgaaaaat gccatcgact ggctttcccg
     1381 cctgccggat caaccgctgg ccggtaaacc ggtgttgatt cagaccagct caatgggcgt
     1441 gattggcggc gcgcgctgtc agtatcacct gcgccagatt ctggttttcc tcgatgcaat
     1501 ggtgatgaac aagccggaat ttatgggcgg cgtgattcag aacaaagttg atccgcaaac
     1561 cggagaagtg attgatcagg gtacgctgga ccacctgacc gggcaattga ccgcatttgg
     1621 tgagtttatt cagcgagtta agatctaaat aaaaaacccg ccagtaatca tgcatggcgg
     1681 gtttttaatg cgctatcgat tttaatgagc gtcgataaat acaatcttca ggataaacag
     1741 cagcgcaacg atgattacgc acgggctaag gtcacgcaga cgcccggtac caatcttcat
     1801 cacgcagtag gagataaagc ccagcgcaat accttcggta atcgaaaagc tgaacggcat
     1861 catcacggcg gtaataaacg ccggaacaga ttcagtaaga tcctgccagt tcacgcgagc
     1921 aagacttgag gtcatcaaca cgccaacgta gatcaacgcg ccagctgcag cgtagtctgg
     1981 caccattccc gccagcggcg acagaaagat aaccagcagg aacagcagac caacaaccac
     2041 tgccgtcaga ccggtacgac cgccaaccga tacgccggaa gaggactcaa tataagccgt
     2101 aacggaagaa gtaccgataa acgaaccggt cacggaagag atactgtcga catacagcgc
     2161 ctgcttcatg cgcgggaatt tccccttctc atccgccaga cctgctttat cggtcacgcc
     2221 aatcagcgta ccggaggagt caaacaagtt gaccaacatg aaagagaaaa tcacccctgc
     2281 cagcccgagg ttaaacgacc cggctaaatc tacatgaccc acaactgtca ttacgctcgg
     2341 cggcgcagaa acgatgccat tgtagtgcac atcacccagc atccagccca gcagcgtcgt
     2401 caccacgata gaaaccagca ccgctgcgtg aatgttgcgc gaggccagaa tagcaatgat
     2461 gaaaaagccg aggataccca gaagtacgct gtgagaagtc agattaccga tgctcaccag
     2521 cgtttccgag ttagcgacaa tcacacctgc gtttttcagc cccatcatgc cgataaacag
     2581 gccgataccg ctggtaatac ccacacgcag acttaccgga atgttagcaa tcatccagta
     2641 acgaacgcgg aaaatggtca aaagaagcag tccgacagcg ccccagaaga ttgcgcccat
     2701 accgatctgc cacggcagcc ccattgcctg gacaaccaca aaagcgaaaa aggcgttaag
     2761 ccccatcgca ggagccagcg ccaccggaag gtttgcaaac aatcccatca taatgctacc
     2821 gaaagcagca atcagacagg ttgttacgaa gacggcactg gtgtccatgc cagcaacgcc
     2881 cagaatttgt gggttaacaa atacgatgta gaccatcgtc aggaaggtgg tgaacccggc
     2941 aatcacttca gttcgtgccg tagtaccatg ttcgcgcagt ttaaaaacac gttccagcat
     3001 cctctgacca gaagtctggg
//
LOCUS       DAJTYH010000218         2948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTYH010000218 DAJTYH010000000
VERSION     DAJTYH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            100..927
                     /locus_tag="OO609_004041"
     CDS             100..927
                     /locus_tag="OO609_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6635233.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            971..1720
                     /locus_tag="OO609_004042"
     CDS             971..1720
                     /locus_tag="OO609_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6635234.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1717..2286
                     /locus_tag="OO609_004043"
     CDS             1717..2286
                     /locus_tag="OO609_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635235.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2410..2652
                     /locus_tag="OO609_004044"
     CDS             2410..2652
                     /locus_tag="OO609_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6635236.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2656..2802
                     /locus_tag="OO609_004045"
     CDS             2656..2802
                     /locus_tag="OO609_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635237.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            2811..>2948
                     /gene="xisR"
                     /locus_tag="OO609_004046"
     CDS             2811..>2948
                     /gene="xisR"
                     /locus_tag="OO609_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR6635238.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HV"
BASE COUNT          791 a          710 c          779 g          668 t
ORIGIN      
        1 ggatacggta gcagtgattt ctggctttgc tctggcgtgt ctgaccccgc cctgcattta
       61 tgccatttat aagggtcttt ctttttctaa ggataaaaga tgaaaaaagt tattcctgct
      121 ttagcccttg ttctgctgac caccggactt gtcggttgcg atcgcgttga gcctggtaat
      181 gttggcatca aggtaaacaa actgggcgac gataaaggcg tcggagaagt ggtcggtgtt
      241 gggcgctact ggacgggatg gaacactgag gtttacatct tcccgacctt caaacaaatg
      301 aagacctacg atgagccatt cagcttccag atgagtgacg gtacaaccat cggctatcac
      361 atcggcgtgg cctacaaagt tgatccatcc aaagttacca cagtctttca gacctaccgc
      421 aaaggcgtgg acgacattac cgacaccgac ctgcgccaga agatagccga cgcactcaac
      481 cgactggcca gcaaaatgac caccgacaaa tttatcgacg gcggcaagtc tgagctgctg
      541 gatgcagctc ttaaagacat tcaggaagaa atgacgccca tcggtattca ggtaatgagc
      601 ctctcatatg tgggtaagcc ggagtacccg cctactgtta tcgacagcat taatgccaaa
      661 gtcacggcga accagaaaac cctgcaatgc gagcaggaag taaaacagcg cgaagcggaa
      721 gccaacatgt tgcgcgcgga agctgccgga caggcagatg cgattcgcac aaaagcccag
      781 gccgaagccg atgctattcg tttacgcggt gaagctctgc gccagaaccc aaacgttatg
      841 gagctggaag ccatcaacaa gtggaacggt acactgccgc aatacatgac cagcggtgcc
      901 aacacaccat ttattcaggt taagtaatac atatgcccgg tattacacgc cgggctgtct
      961 ggagataaaa atgaatactg taaccatcaa caacaaacag tttccggtaa tcgaatatcg
     1021 cggtcagcgt gttgtgacgc tggcgatgat tgatgaagtc caccagcgac cggaaggaac
     1081 cgcgcgtgca gcattcaacc gaaaccgtga gcatttcatc agcggtgtgg attatgccga
     1141 attaggtgcg gacgtaatac gtacggacct cccggaagga acattctcta aatttgcacc
     1201 atcggggatt gtactttttg aatcaggtta cctgatgttg acgaagccat tcaatgacgc
     1261 ccttgcctgg caggttcagc gcgaactggt taacagctac ttccgcactc gcacaccgct
     1321 aacggaaatc gagatgatcg ctgcgatagc cgccgacgct gttcgtcaac agaagcgcct
     1381 gaatcatgtg gaagaacagc tcgaaacagt cacagaagcg gtggaaacca tcaaacgcgg
     1441 aaatatgcgc gccggatatg tcggttaccg ccaggtggta gccaaaagtg gaatgagtga
     1501 caccaagtgt cggaatctgg tgaacgcata tcaaattcct acagacaccc acgaattcat
     1561 gactccggac ggtctgttgt ctcgcagggc tatcgtcgag cttgagccat ttatggcggc
     1621 gttccaccaa atgatgtcag aggctgaccc acgcggcaca cgctggtatc acccgaagat
     1681 ggggcttttc caggtgatcg gatgggagga taaagcatga tcatccagtc aaaacttatt
     1741 cgcgccgctc tggtgtgcgc tgctaaaaac gacgttcgtt attacctgaa cggtcttcac
     1801 atcacgccga aacatattga ggcaaccaat ggttccgtgg cactgcgcat ggctcacggc
     1861 atccggacga agaaaaacat cattgtccag ttcgaagggg gcgtcccggc caaagccgaa
     1921 acgacagagc tgatttttag taaagagccg atcgctgttc atcgcgacca gtttcagcga
     1981 cgactgtcca ttaccggcat taaactggtg gacggttgtt ttccggattt ggatcgcatc
     2041 attccgaaaa agtttgaccg ctgtactcac ccggtgctcc aggcgggtta cctgagttat
     2101 ccagagaaga tgtttggtcg tgagcgtaaa tttattcccg tccagttacg tccctccggt
     2161 gacgggcaag cggtcagaat tcagtttgat tccatcatca actcaatgta tggcaatcct
     2221 gaatttgttg tgatgccttg tcgtgatcat ggcgatttca acgtggctca ggagcatccg
     2281 gaatgaaaat cgaataccag gacgccgcag gaggtgaatc aatgagctgg cctgatgcaa
     2341 tcgtaactct ggggatggta ttcgcagtag cgtttgttgt gtactcgatt tgtcgatggg
     2401 gataaccaca tgttcgcttt gattcaacgc gggcagatat acacagacag agccggatac
     2461 ccagtagaaa ttactcgcag tactgagcac tcagtgttct ttcggaggat ggacggacgt
     2521 accgggcgag tacgcatcgg tgaattcagt agcctgttcg agcatattga ccacctggaa
     2581 tatcacaaga ttctggctga aacagaacaa gaaaagcacc tgaagaaatt acgcgcaatg
     2641 caaaggaagt aaaaaatgaa taaagcgttt gagctatggg tccaccagcg ttacggcaac
     2701 cactacgacc tgacgcgaga tgttgacggt ttctactgtc gtgaaattgt gaaacgaatg
     2761 tttgaggtct ggtgccactg ccgtgggctg agtgttgtgt gaggtaatgc atgggcaatg
     2821 tgattcaact ggctcccaat gaatgggttt gtgaaagcgt tctaatcgca attaccgggc
     2881 tcaaaccagg cacaattctt cggacccgga aagaatgctg gatggttgga agagagtata
     2941 ttcacgta
//
LOCUS       DAJTYH010000219         2833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTYH010000219 DAJTYH010000000
VERSION     DAJTYH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..2645
                     /locus_tag="OO609_004047"
     rRNA            <1..2645
                     /locus_tag="OO609_004047"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2741..>2833
                     /gene="rrf"
                     /locus_tag="OO609_004048"
     rRNA            2741..>2833
                     /gene="rrf"
                     /locus_tag="OO609_004048"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          736 a          626 c          891 g          580 t
ORIGIN      
        1 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
       61 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      121 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      181 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      241 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      301 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      361 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      421 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      481 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      541 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      601 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
      661 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
      721 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
      781 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
      841 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
      901 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
      961 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1021 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1081 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1141 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1201 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1261 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1321 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1381 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1441 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1501 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1561 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1621 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     1681 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     1741 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     1801 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     1861 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     1921 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     1981 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2041 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2101 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2161 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2221 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2281 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2341 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2401 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2461 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2521 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2581 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     2641 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     2701 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     2761 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     2821 ggggtctccc cat
//
LOCUS       DAJTYH010000220         2794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTYH010000220 DAJTYH010000000
VERSION     DAJTYH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..770
                     /locus_tag="OO609_004049"
     CDS             <1..770
                     /locus_tag="OO609_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6635239.1"
                     /translation="VPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVP
                     QPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVA
                     WINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMR
                     YDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYA
                     HLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1119..2291
                     /locus_tag="OO609_004050"
     CDS             1119..2291
                     /locus_tag="OO609_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6635240.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2345..>2794)
                     /locus_tag="OO609_004051"
     CDS             complement(2345..>2794)
                     /locus_tag="OO609_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6635241.1"
                     /translation="VTVILNGTTFQSAGDYQPSAEQPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT          883 a          567 c          554 g          790 t
ORIGIN      
        1 ctgttccaga atacacgcca aaaccagcgt ccgttgcaac gaaggctacg catctttcat
       61 ttataaaggc cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa
      121 ttattaaagt gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac
      181 aaaggctgat tgatgaatgt ccggagccat taaagtctgt tgttgaattt gcactggcaa
      241 caggcttaag acgctcgaac atcatcaacc ttgaatggca acaaatagat atgcagcgcc
      301 gggtggcatg gataaacccg gaagagagta aatcaaaccg cgcaattggc gttgcgctga
      361 atgatactgc atgtcgcgta ttgaaaaaac aaatcgggaa tcatcaccgt tgggtatttg
      421 tgtacaagga aagctgtacc aaaccagacg gaacgaaagc gccaacggtc aggaagatgc
      481 ggtatgacgc aaacacagcc tggaaagcgg cgctgagacg agcaggtatt gatgatttca
      541 gatttcacga cttgagacac acctgggcaa gttggctggt tcaagccgga gtcccgttgt
      601 cagtgttaca ggaaatggga ggctgggagt ctatcgaaat ggttcgtcga tatgctcacc
      661 ttgcacctaa tcaccttacc gaacacgcac ggcaaataga ctcgatcctg aacccatcgg
      721 tcccaaattc gtcccagtca aaaaataagg aaggtactaa tgatgtgtaa cttattgatt
      781 taaatggtgc cgataatagg agtcgaacct acgaccttcg cattatgaat ctgtagcacc
      841 aatcataact atctgtttta gcaagcatta accgcattca ctaagctata gttgatgaca
      901 caaacagaaa gttgatgcat gatgttgcca tatatatgtc acaaatacgg cacaacgatc
      961 ttcaaacata tagccacaca ttcacagaag agcacaaagc cttgcaatcc agtgcaaagc
     1021 ttggtgtgtc tccgttttgt ttcatcagtt acacataact atatgatcat taactaaaag
     1081 tcagtatact tggagaaatt ttaattgcgt aatagagcat gcatatgatt gaaattaaca
     1141 gtttattatt aataacatcc gtgatattga tgtcgttatt agctgtgggg ttatttgata
     1201 aaatttcccc aataaatctt gttgagcacg gcaggaataa tcaaatagat ggaatgcggg
     1261 ggttcttagc aattttcgtg cttattcatc acgcagcaat ttggaatgga tacttgtcat
     1321 ctggagtatg ggaagcacct tcatcaaatc tgttagcaaa tttaggccaa gttggtgtgt
     1381 cattcttttt tatgattact ggttatctgt tcttttcaaa gattatctct ggagatcagg
     1441 actggacaag gctttatgta tcaagattac tacgattaac cccaatgttc atagttagcc
     1501 tatgtctcat ttttatcatt gtaggtttta agtctggatg gagaatgcag gtatccacag
     1561 aagagctttt tgtgtcaata atgaagtggt tgccattcac tgcgctaggt atgccgaaca
     1621 ttaatgacgt aaaagattca tttactatca atgccgctgt aacatggaca cttgtatatg
     1681 aatggttctt ttatttttct cttccggtaa tttccgcgct cataaaaaga aaagtcagta
     1741 tttacatggt tatgattagc gcaatatcgc tatttgtttt cattttattt ttcagtaaaa
     1801 tacacatagc atcatttctt tttgggctcc tggcattttt gctaaataaa tcaaagatag
     1861 tcaatggaat tgccaaagca aaagtaacac caattataat aactgcgata atggtttttg
     1921 aaatgacgta tttcaaaaca acttacgcac cgctaccact gattctttgt ggaataacat
     1981 ttatcattat cgcatcaggt tgtgatttgt atggaatatt aagattaaat ataaccagaa
     2041 agctaggaga aactacctac agcgtttacc ttctgcatgg gatattcctt tattgcctaa
     2101 tgacgtggat tattcctaat aattacactg aaaatacatt tatcatactg gtaagcacaa
     2161 ctgcatttct tattacgttc acatcatgcc taacttttaa attaatagag acgccattca
     2221 tcaaattaac aaaacaaacc acaactttag taaaagaatt aatacccaca ttaacaaaca
     2281 acaatcaata aacaataaac ataagccgcc ctacatatat gtccaaatgg ggcggcgaat
     2341 gtatttatat tagtctggta atgctaaatg caaacgaact ggagattcct gccccgctaa
     2401 gcgtattgcg cagggtcacc ctaaatgtat ctacgccaat ataagtaaca tcacaactaa
     2461 ctgaatcagg cgcagatata ggtgttatct gcacaatata accgctagca actgctgcga
     2521 gcggatgaga aaagctaaca tcatacacac ctgtagaaac cacgttaacc gatgaaacac
     2581 cagtagcaac gtcgatacta ccattggcgt taatttttcc cgagcgtgtg actgacaatg
     2641 cagaagccga tgttacctta ctggttgttt ctgtaaaaac acacccaatg tcaactacct
     2701 cgcaattagc tcctgttatc cagtatggtt gttctgcact tggctgataa tcccctgcgc
     2761 tctggaacgt tgtgccgttg agtatgactg taac
//
LOCUS       DAJTYH010000221         2783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTYH010000221 DAJTYH010000000
VERSION     DAJTYH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..820)
                     /gene="hypT"
                     /locus_tag="OO609_004052"
                     /gene_synonym="qseD"
     CDS             complement(<1..820)
                     /gene="hypT"
                     /locus_tag="OO609_004052"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6635242.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPI"
     gene            complement(885..2057)
                     /gene="iadA"
                     /locus_tag="OO609_004053"
     CDS             complement(885..2057)
                     /gene="iadA"
                     /locus_tag="OO609_004053"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6635243.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(2070..2531)
                     /locus_tag="OO609_004054"
     CDS             complement(2070..2531)
                     /locus_tag="OO609_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6635244.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(2528..>2783)
                     /locus_tag="OO609_004055"
     CDS             complement(2528..>2783)
                     /locus_tag="OO609_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635245.1"
                     /translation="MTKELAQEGEITERDKVIFAAYQTSGSAIITNYFSSGVAVFAFL
                     GTSVIVPLAVILVFKFVGANILRVWLNFEERRNPTQGAQA"
BASE COUNT          658 a          744 c          749 g          632 t
ORIGIN      
        1 gaatcgggat cacctgttcg tcccgattca gcacaacgag ctgcccgctg cgaatttctt
       61 gttggatggc atactccggc agccaggcaa tcccacagcc gtcgagggca acctgcttta
      121 aaagctcgct catcgaagag acaaaaaagg tgctgaaact taactcactg tggcgcgtca
      181 gggtgcgatt aatcaatcgc accatgtagg agttgcggct gtaattcagt aacggaaagt
      241 gtggctgcgc gaggttaaaa agtgcttctc cgtgttcgtc actggcgcag acagggaaca
      301 attgagattc aaataagcga atgtggtcaa acggcgcttc cagcaaatct tcgtcgtgaa
      361 aggaaaaaat acagtcactt tgcccttcac gcagtttatc gaccgcttca tcgacatcaa
      421 tggcttcaat tgcccaggta aagagcggcg gcatctggct gataatggac ggtaacagac
      481 cgagggaaag agagtgtgca gcggctatct tgattttacg ttgcgcgtaa tcgctgccgc
      541 cacgcagctc tgcaaggttg ctttctaact gttgcaacag atggcggatc tgcgaatgga
      601 agattttccc ttgttctgag agttgcagtg gcgtcacctg gcggttaaac aattcaacgc
      661 caatcgcctg ttccagtgca cggatgcggc ggctgaatgc cggttgcgag acgttgcgac
      721 tgactgccgc ctgggaaaaa ttgcggcatt tttccagagt cagaaaatca taaagccatt
      781 tggtttcaat attatgcaaa accgcaccac agtcatccat tacgttttac tctgtcagcc
      841 gcccttgggg gcattctacg tccattaggg cggctgacaa ccgtttaagc cgtttcaaac
      901 gttcctttca cgcaggcttt gccgtctttg accatcagtt tgccgcgagc gtatacctgc
      961 tcaatgcgca attctggcgt catgaccagt aagtctgcgt cattgcctgg cagaatttcg
     1021 cctttcccgc tcatgttaag gaaaccggct acgctactgg tgagcgggcg cagggcatcg
     1081 ctgatactga aatcatagtc tttgaccagc acctgcacgg tttccagcaa cgtttcaaaa
     1141 cctgcaacac cgatatgggt taaattccct tcgtcatcga agaacggctg gctaccgttg
     1201 ccgtcggagc tgagggtgac gcgtgccagc ggaatacccg cctgaacggc gcgggcaata
     1261 ccttcggcag gggcgaccgg ttcgtcaatg ctgctggtga tatcgatggt gccgcctttg
     1321 cgcgcgaact ccagcgcctg ctcaaacaac ggtacgttgc ggttaacgtg ggtcggcagc
     1381 agcttgctga tcggcacatc gcagttttcc agcaggtcat aaataggctt taacgccttt
     1441 ttactgtcgc ccatgtggaa cacggtgacg ccaggtttac cgccgagcaa accgccaacg
     1501 cgggattctg ccgccatatt agccaggtga taaacgtccg gtgcggcaga acggtgatca
     1561 gagatggcgc atttcacgcc aatcacacga tcgataatcg ccacgtcttt ttccacggaa
     1621 cccgtaatag tgcgggaagg gacatgataa gcgccggtca gcatccaggc gctgatgcct
     1681 tcttcattga gcgcacgggt cttggcgagc agggattccg ggtggcgaga gatagagtcg
     1741 gtgcccagca gaccaaccac tgacgtgacg cccgcttccg tcaggcgact tagcgccact
     1801 tccggcgtgc gcgtcgtggg acctgcttcg ccgccaccgc caatcaaatg gacgtgttga
     1861 tcaataaaac ctgggcagag gatctgcccg ctgagatcga caaccgtgca gttcggtaca
     1921 atgtcagaag ggatattgct ggcaacggcg ataattttgc cgttagcgac gaggacatcg
     1981 caaattcccc gatcttccgg cgcatacaaa tgcgctccct gcagcagggt aaaaccggct
     2041 gcggtataat caatcatggt aactccttgt taaacaataa gctgcataac ccagatcgac
     2101 agcaatgcgt taatcacgca gaccgtaatg atatgcgggt aatatttggc attcacttcg
     2161 gctgtcccca ggcagcgacc gacattttgc accgggttgc ccattaaata cattgcgggc
     2221 agtaaaacgg tgacatcgtg gccggttaat gcgtcagcgg ttgccagact ggcggcaaca
     2281 ccgactgcgc cgcccatact cattaatgcg gctaaaagga cggttgccgc ttcaccgggt
     2341 aatccccaca gcgccattac gggttcacag atatgcccca cccagtcgag caggccggta
     2401 attttcagcg cctgaatgat gacaaacgcc atcaccacgt ttggcagcag gttggtcgtg
     2461 gcgatagtaa agccacggcg cgcgccatcg ataaacatat ccatgacgtt tttgcggacc
     2521 tgagttgtca tgcttgcgct ccttgcgttg ggttgcgacg ttcttcgaag ttaagccaga
     2581 cgcgcaaaat gttggctccg acaaatttaa acaccaggat gacggctaaa ggtacgatta
     2641 cggacgtgcc cagaaaggca aacaccgcga cgccggaaga gaagtagttg gtgatgatgg
     2701 cgctaccgct ggtttgatat gcggcgaaaa tcactttatc gcgttcggta atttcacctt
     2761 cctgcgccag ctctttggtc ata
//
LOCUS       DAJTYH010000222         2684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTYH010000222 DAJTYH010000000
VERSION     DAJTYH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..198
                     /locus_tag="OO609_004056"
     CDS             <1..198
                     /locus_tag="OO609_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6635246.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            237..1040
                     /gene="agaC"
                     /locus_tag="OO609_004057"
     CDS             237..1040
                     /gene="agaC"
                     /locus_tag="OO609_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR6635247.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1030..1821
                     /gene="agaD"
                     /locus_tag="OO609_004058"
     CDS             1030..1821
                     /gene="agaD"
                     /locus_tag="OO609_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR6635248.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1822..2577
                     /locus_tag="OO609_004059"
     CDS             1822..2577
                     /locus_tag="OO609_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR6635249.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          663 a          626 c          674 g          721 t
ORIGIN      
        1 aaattggtag aaggtggcat tgacctgaaa gatgtcaacg tcggcaatat gcatttctcg
       61 gaagggaaaa agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta
      121 cgttttatta aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa
      181 gaacaaatcc ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc
      241 atgaaataac cctacttcag ggattatccc tagcggcgtt agttttcgtt ctggggattg
      301 atttttggct ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg
      361 ctattctcgg tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg
      421 ccggattaac ccctgcaggt ggtgttcagc cgcccaaccc gattatggca ggtctgatga
      481 ccaccgtcat tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc
      541 cgtttagttt gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta
      601 tgaccaaagc cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact
      661 ggacaacgat gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc
      721 tggcacaagg ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct
      781 ttgaagtggc tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt
      841 tcaaagcgca atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccggg
      901 tcagcaacct gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca
      961 atgcgaaatc ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg
     1021 actacagcaa tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg
     1081 tcgctgctgc aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg
     1141 atgttgccga tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg
     1201 aaagataacc tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta
     1261 ttaatttcga tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca
     1321 ctgtttggcc caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt
     1381 atggcgggaa tttgctcatc atttgccagc cagggaaacc tgctggggcc gattctattt
     1441 ttcgccgttt acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca
     1501 gtcggcgtga aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca
     1561 accatcctcg ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg
     1621 gtgacatcgt ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat
     1681 aaagtcttcc cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg
     1741 gtgaaaaaag cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt
     1801 tccgcattcg gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt
     1861 aaggaatttc atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa
     1921 cgagccagcg aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg
     1981 gcgaccggag ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag
     2041 caggttgata tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta
     2101 acgatgcctg gcacctgcga aactttccta caacagcata tcgtgcagcc gctggggcta
     2161 cgtgaagacc agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg
     2221 gtaacgaacc tgattgcgcg caaaggcggt ctggatttat gcgttctcgg attggggaaa
     2281 aacggtcatc ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt
     2341 caacttgatg ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact
     2401 cgtgggatca ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg
     2461 actggcgaag gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct
     2521 atcccggctt catttttatg gctgcacagt aactttattt gtttaattaa tacctaacca
     2581 gatgaaaaat ttgctcaaaa tttttaaatt aacttattca acagtcacgc attatattaa
     2641 ataacatttg actgggttga acataacgcc aatagcaaaa ggag
//
LOCUS       DAJTYH010000223         2646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTYH010000223 DAJTYH010000000
VERSION     DAJTYH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>2646
                     /gene="hrpA"
                     /locus_tag="OO609_004060"
     CDS             <1..>2646
                     /gene="hrpA"
                     /locus_tag="OO609_004060"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6635250.1"
                     /translation="YRTKVQRLPIEPISQASANQRKGRCGRVSEGICIRLYSEDDFLS
                     RPEFTDPEILRTNLASVILQMTALGLGDIAAFPFVEAPDKRNIQDGVRLLEELGAITT
                     DEQASAYKLTPLGRQLSQLPVDPRLARMVLEAQKHGCVREAMIITSALSIQDPRERPM
                     DKQQASDEKHRRFHDKESDFLAFVNLWNYLGEQQKALSSNAFRRLCRTDYLNYLRVRE
                     WQDIYTQLRQVVKELGIPVNSEPAEYREIHIALLTGLLSHIGMKDADKQEYTGARNAR
                     FSIFPGSGLFKKPPKWVMVAELVETSRLWGRIAARIDPEWVEPVAQHLIKRTYSEPHW
                     ERAQGAVMATEKVTVYGLPIVAARKVNYSQIDSALCRELFIRHALVEGDWQTRHAFFR
                     ENLKLRAEVEELEHKSRRRDILVDDETLFEFYDQRISHDVISARHFDSWWKKVSRETP
                     DLLNFEKSMLIKEGAEKISKLDYPNFWHQGNLKLRLRYQFEPGADADGVTVHIPLPLL
                     NQVEESGFEWQIPGLRRELVIALIKSLPKPVRRNFVPAPNYAEAFLGRVTPLELPLLD
                     SLERELRRMTGVTVDREDWHWDQVPDHLKITFRVVDDKNKKLKEGRSLQDLKDALKGK
                     VQETLSAVADDGIEQSGLHIWSFGQLPESYEQKRGNYKVKAWPALVDERDSVTIKLFD
                     NPLEQKQAMWNGLRRLLLLNISSPIKYLHEKLPNKAKLGLYFNPYGKVLELIDDCISC
                     GVDQLIDANGGPVWTEEGFAALHEKVRAELNDTVVDIAKQVEQILTAVFNINKRLKGR
                     VDMTMALGLSDIKAQMGGLVYRGFVTGNGFKRLGDTLRYLQAIEKRLEKLAVDPHRDR
                     AQMLKVENVQQAWQQWINKLPPARR"
BASE COUNT          641 a          669 c          759 g          577 t
ORIGIN      
        1 gctatcgcac caaagtgcag cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc
       61 agcgtaaagg ccgctgtggt cgtgtgtccg aagggatctg tattcgtctc tattccgaag
      121 acgatttcct ctcgcgcccg gagtttaccg atccggagat tctgcgtacc aacctggcct
      181 cggttatttt gcagatgacc gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg
      241 aagcaccgga taaacgcaat atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga
      301 tcaccacgga tgaacaggcc agcgcctata aactgacgcc gctcggtcgc cagctctcgc
      361 agttgcctgt cgacccacgt ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg
      421 tgcgtgaggc gatgattatc acgtccgcgc tctccatcca ggatccgcgc gaacgtccga
      481 tggacaaaca gcaggcatcg gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact
      541 tcctcgcgtt tgtaaatctg tggaattatc ttggcgagca gcaaaaggcg ctttcttcca
      601 acgccttccg tcgcctgtgt cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc
      661 aggatatcta cacccagttg cgccaggtgg tgaaagaact tggcattccg gtcaacagcg
      721 aaccggcgga gtatcgtgaa attcacatcg ccttactgac cggtttgctt tcccatatcg
      781 gcatgaaaga tgccgataaa caggaatata ccggtgcacg taacgcgcgt ttctccatct
      841 tccccggttc tggtttattc aaaaagccgc cgaaatgggt aatggtggcg gaactggtag
      901 aaaccagccg cctgtggggg cgcattgctg cgcgtatcga cccggaatgg gtagaacccg
      961 ttgctcagca tttgattaaa cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg
     1021 cggtgatggc aacggaaaaa gtcactgttt atggtttgcc gattgttgct gcgcgcaagg
     1081 tcaactacag ccagatcgat tcggcgttat gtcgtgaact ctttattcgc cacgctctgg
     1141 tggaaggtga ctggcagacg cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg
     1201 aagtggaaga gctggaacac aaatcacgtc gccgcgatat tctggttgat gacgaaacgc
     1261 tgtttgagtt ctacgaccag cgcatcagcc acgatgtaat ctccgctcgt cacttcgata
     1321 gctggtggaa aaaagtcagc cgcgaaacgc ctgatttgct caactttgaa aagagcatgt
     1381 tgatcaaaga aggggcggaa aaaatcagca agctggatta cccgaacttc tggcatcagg
     1441 gcaatctcaa gctgcgtttg agatatcagt ttgagccagg cgcggacgcg gacggtgtga
     1501 ccgtacatat tccgctgccg ttacttaacc aggttgagga aagcgggttt gaatggcaga
     1561 tccccggtct gcgccgcgaa ttggtgattg ctctgattaa atcgttgccg aaaccggtac
     1621 gccgtaattt tgtgcctgca ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc
     1681 tggagttacc gttgctcgac agccttgagc gcgagttacg gcggatgacc ggcgttaccg
     1741 ttgaccgcga agactggcac tgggatcagg tgcccgatca cctgaaaatc accttccgcg
     1801 tggtggatga caaaaacaag aagctaaaag aagggcgctc gctacaagat ctgaaagatg
     1861 cgctgaaagg caaagtgcag gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga
     1921 gcggcttaca tatctggagt tttggtcagc tgccggaaag ttacgaacag aagcgtggca
     1981 actataaagt gaaggcgtgg ccggcgctgg tggatgaacg cgacagcgtg acgatcaaac
     2041 tgtttgataa cccgctggag caaaaacagg caatgtggaa tggtcttcgc cgtctactgc
     2101 tgctaaatat ttcctcgccg atcaagtatt tacatgagaa gttaccaaac aaagccaagc
     2161 tggggctgta ctttaacccg tatggcaaag tgctggagct gatcgacgac tgtatctcct
     2221 gcggtgtgga tcaactgatc gacgccaatg gtggcccggt ctggacggaa gaaggctttg
     2281 ctgcgctgca tgaaaaagtg cgcgccgaac tgaatgacac ggtggtggat attgcgaagc
     2341 aggtcgagca aatcctcact gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg
     2401 atatgaccat ggcactgggg ctttctgata ttaaagcgca gatgggcggg ttggtatatc
     2461 gcggttttgt cactggtaac ggcttcaaac ggctgggcga cacgctgcga tatttgcagg
     2521 cgattgaaaa acgactggaa aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc
     2581 tgaaagtcga aaacgtccag caggcgtggc agcaatggat caacaaactg ccgcctgcac
     2641 gtcgtg
//
LOCUS       DAJTYH010000224         2614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTYH010000224 DAJTYH010000000
VERSION     DAJTYH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..501
                     /gene="ssuE"
                     /locus_tag="OO609_004061"
     CDS             <1..501
                     /gene="ssuE"
                     /locus_tag="OO609_004061"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6635251.1"
                     /translation="KLNGLDVEVYHWNLQNFAPEDLLYARFDSPALKTFTEQLQQADG
                     LIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLATGGTVAHLLAVDYALKPVLSA
                     LKAQEILHGVFADDSQLIDYHHRPQFTPNLQTHLDTALETFWQALHRRDVQVPDLLSL
                     RGNAHA"
     gene            494..1453
                     /gene="ssuA"
                     /locus_tag="OO609_004062"
     CDS             494..1453
                     /gene="ssuA"
                     /locus_tag="OO609_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6635252.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1450..2595
                     /gene="ssuD"
                     /locus_tag="OO609_004063"
     CDS             1450..2595
                     /gene="ssuD"
                     /locus_tag="OO609_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6635253.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
BASE COUNT          599 a          721 c          710 g          584 t
ORIGIN      
        1 aaactaaatg gcctagatgt agaggtttat cactggaatc tgcaaaactt cgccccggaa
       61 gatctgcttt atgctcgttt cgatagtccg gcactcaaga ccttcaccga acagctgcaa
      121 caggctgatg ggctgattgt cgccacgcct gtgtataaag ccgcctattc cggcgcgctg
      181 aaaaccctgc tcgacctgct gccagagcgc gctttgcaag gcaaagtggt gctaccgctg
      241 gcgacgggcg gtaccgtggc ccatctgctg gcggtcgatt atgcccttaa accagtttta
      301 agcgcactga aagctcagga gatcctgcac ggcgtgtttg ccgatgactc acaattaatt
      361 gattaccatc acagacccca gttcacgcca aatctgcaaa cccatcttga taccgcgcta
      421 gaaactttct ggcaggcatt gcaccgccgc gatgttcagg ttcctgacct tctgtctctg
      481 cgaggtaatg cccatgcgta acatcattaa actggcgctg gcgggattgc ttagcgtctc
      541 tacttttgcg gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg
      601 cagtattggt atggtgctgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc
      661 aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg
      721 cagtattgat ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc
      781 tgatttggta tatgtgggcg tcgagccacc gaagcccaaa gccgaagtga ttctggtggc
      841 agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa
      901 aggttccagt tcacacaacc ttttactgcg tgcactgcgt caggccggac ttaaattcac
      961 cgatatccag cccacttacc tgacgcctgc cgatgcccgc gccgcgttcc agcaaggtaa
     1021 cgttgacgcc tgggctatct gggatcccta ctactccgct gcgttattac agggcggcgt
     1081 gcgggtgctg aaagacggca ccgatctcaa tcaaactgga tcgttttatc tggcagctcg
     1141 tccctatgca gaaaaaaacg gcgcttttat tcagggcgta ctggcaacct ttagtgaggc
     1201 cgatgcgtta acccgcagcc agcgcgaaca aagcatcgct ttactggcaa aaacgatggg
     1261 cttaccggca ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc
     1321 ggttaacgcc gaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaaccg
     1381 tctggtgccg aaaaaagtcg atattcgcca gcgcatctgg cagcccactc aactggaagg
     1441 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccacggtga cgggcactat
     1501 ctgggaacgg aagaaggttc acgcccggtt gatcacggtt atctgcaaca aattgcgcaa
     1561 gcggcggatc gtctgggcta taccggtgtg ctgatcccaa cggggcgatc gtgtgaagat
     1621 gcgtggctgg ttgcagcgtc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1681 ctgcgtccca gcgtaacctc acctactgtt gccgcccgcc aggccgccac gcttgaccgt
     1741 ctctcaaatg gtcgtgcgtt gtttaatctg gtcacaggca gcgatccaca agagctggca
     1801 ggcgacggcg tgttccttga tcatagcgag cgctacgaag cctcggcgga atttacccag
     1861 gtctggcggc gcttattgct gggggaaacc gtcaatttca acggtaaaca tattcatgtg
     1921 cgcggagcaa aactgctctt cccgccgatt caacagcctt atcctccgct ttactttggc
     1981 ggatcgtcag atgtcgccca ggagctggcg gcagaacaag ttgatctcta cctcacctgg
     2041 ggcgaaccgc cggaacttgt taaagagaaa atcgaacaag tgcgggcgaa agctgccgcg
     2101 tatgggcgca aaattcgttt cggtattcgt ctgcatgtga ttgttcgtga aactaacgac
     2161 gaagcgtggc aggccgccga gcggttaatc tcgcatcttg atgatgagac tatcgccaaa
     2221 gcacaagccg cattcgcccg gacggattcc gtaggacaac agcgaatggc ggcgctacat
     2281 aacggcaagc gcgacaatct ggagatcagc cccaatttat gggcgggcgt tggcttagtg
     2341 cgcggcggtg ccggaacggc gctggtgggc gatggtccta cggtcgccgc gcgaatcaac
     2401 gaatacgccg cgcttggcat cgacagcttt gtgctttcgg gctacccgca tctggaagaa
     2461 gcgtatcggg ttggcgagtt gctgttccca catctggatg tcgccattcc ggaaattccc
     2521 cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcaa atgattttat cccccgtaaa
     2581 gtcgcgcaaa gctaaggaac acgatgatgg caac
//
LOCUS       DAJTYH010000225         2602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTYH010000225 DAJTYH010000000
VERSION     DAJTYH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            30..1898
                     /gene="kup"
                     /locus_tag="OO609_004064"
     CDS             30..1898
                     /gene="kup"
                     /locus_tag="OO609_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6635254.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2065..2484
                     /gene="rbsD"
                     /locus_tag="OO609_004065"
     CDS             2065..2484
                     /gene="rbsD"
                     /locus_tag="OO609_004065"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6635255.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
BASE COUNT          571 a          628 c          684 g          719 t
ORIGIN      
        1 catttcatat ttcaacgaaa gactagtcta tgagcactga taataagcaa tcattgcccg
       61 cgattaccct cgcggcgatt ggagttgtct acggcgatat tggtaccagc ccgttatata
      121 cacttcgtga atgtttgtcc ggccagtttg gttttggcgt tgaacgcgat gccgtgtttg
      181 gctttttatc gctgatcttc tggctgctaa tctttgtggt ttccattaaa tatctcacct
      241 tcgtgatgcg ggcagataac gccggtgagg gggggatcct gacgttgatg tcgcttgccg
      301 ggcgtaatac gtcggcgcga accacatcaa tgctggtgat tatggggcta atcggcggca
      361 gctttttcta tggtgaagtc gtcataacac ccgctatttc ggtgatgtca gccattgaag
      421 gtctggaaat cgtcgccccg cagctggata cctggatagt tcccctctca attatcgttc
      481 tcactttatt atttatgatt caaaagcatg gcacggctat ggtcggtaag ctgtttgcgc
      541 cgatcatgct gacctggttt ttgattctgg caggactggg gttacgtagc attattgcta
      601 acccggaagt gctgcatgcg ctgaatccga tgtgggcggt gcatttcttc cttgaataca
      661 aaacggtttc ttttattgca ttaggggcag tggtgctgtc gattacgggg ggcgaggtgc
      721 tgtatgctga tatggggcac tttggtaagt tctctattcg cctggcgtgg ttcaccgtcg
      781 tattaccttc cttgaccctt aattacttcg gtcagggagc gttgttgtta aagaacccgg
      841 aagcgattaa gaacccgttc ttcctgttgg caccggactg ggcgctgatc ccactgctga
      901 tcatcgccgc actggcgacg gtaattgcct cgcaggcggt tatctctggc gtcttctcat
      961 tgacgcgtca ggcggtacgt ctgggatatt tgtcgccgat gcgcattatt cacacctccg
     1021 aaatggagtc agggcaaatc tatattccgt ttgtgaactg gatgctctat gtcgcggtcg
     1081 tgattgtgat tgtcagcttt gagcactcca gcaacctggc ggcggcgtac gggattgcgg
     1141 tgaccggaac catggtactg acgtctattc tctcgactac cgtggcacgt cagaactggc
     1201 actggaataa gtattttgtt gcgctgatcc tgattgcttt cctttgtgtc gatattccat
     1261 tgttcaccgc taacctcgat aaactgctct ccggcggctg gttgccattg agcctcggta
     1321 ctgtgatgtt tatcgtgatg accacctgga agagcgagcg tttccgcttg ctgcggcgga
     1381 tgcatgaaca tggtaactct ctggaagcga tgattgcttc gctggagaaa tcaccgcctg
     1441 ttcgcgtgcc cgggaccgcg gtgtatatgt cgcgtgcaat caacgtcatt ccctttgcgc
     1501 tgatgcataa ccttaaacat aacaaggtat tgcatgagcg ggtgattctg ttaactctgc
     1561 gcaccgaaga cgcgccatat gtccataacg tccgtcgggt acagattgaa caactgtcgc
     1621 ccactttctg gcgcgtggtg gcaagttatg gttggcgaga aacgccaaac gtggaagaag
     1681 ttttccaccg ctgcggtctg gaaggattaa gttgccggat gatggaaacc tccttcttta
     1741 tgtcgcatga gtcgttgatc ctcggcaaac gcccgtggta tttgcgtctg cgcggcaagc
     1801 tgtacttgct gctgcaacgt aatgcgctgc gtgcaccaga tcaatttgaa atcccgccaa
     1861 acagggttat cgaactgggt actcaggtcg aaatctaacg ccagacgcct cctttcttca
     1921 taagggggcg tttttgtttt catggttaat caccatgtaa aacgtttcga ggttgatcac
     1981 atttccgtaa cgtcacgatg gttttcccaa ctcagtcagg attaagctgc ggatcagcga
     2041 aacgtttcgc tgatggagaa aaaaatgaaa aaaggcaccg ttcttaattc tgatatttca
     2101 tcggtgatct cccgtctggg acataccgat acgctggtgg tgtgtgatgc tggtttaccc
     2161 atccccaaaa gtacaacgcg tatcgatatg gcattaaccc aaggtgtacc ttcttttatg
     2221 caggtgctgg gcgtcgtcac aaatgaaatg caggtcgagg ctgccattat cgcggaagag
     2281 atcaaacaac ataatccgca actccacgaa acgttgctca ctcaccttga gcagctgcaa
     2341 aaacaccagg gaaataccat tgaaattcgt tacaccacgc atgaacaatt caaacaacaa
     2401 accgcagaaa gtcaggcggt aattcgcagc ggagaatgtt ctccgtatgc gaatatcatt
     2461 ctctgtgctg gcgtgacgtt ctgaggccgt catggaagca ttacttcagc ttaaaggcat
     2521 cgataaagcc ttcccgggcg taaaagccct ctcgggcgca gcgttaaatg tctatccggg
     2581 ccgcgtgatg gcgctggtgg gc
//
LOCUS       DAJTYH010000226         2447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTYH010000226 DAJTYH010000000
VERSION     DAJTYH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(64..822)
                     /locus_tag="OO609_004066"
     CDS             complement(64..822)
                     /locus_tag="OO609_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6635256.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO609_004067"
     CDS             complement(830..1933)
                     /locus_tag="OO609_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6635257.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2447)
                     /locus_tag="OO609_004068"
     CDS             complement(1943..>2447)
                     /locus_tag="OO609_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6635258.1"
                     /translation="LPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSV
                     YTSAFIAEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLN
                     IVKNSSLAAVIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNR
                     RIAIVER"
BASE COUNT          672 a          654 c          570 g          551 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaa
//
LOCUS       DAJTYH010000227         2436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTYH010000227 DAJTYH010000000
VERSION     DAJTYH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            5..643
                     /locus_tag="OO609_004069"
     CDS             5..643
                     /locus_tag="OO609_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6635259.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            646..1809
                     /locus_tag="OO609_004070"
     CDS             646..1809
                     /locus_tag="OO609_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6635260.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1893..>2436
                     /locus_tag="OO609_004071"
     CDS             1893..>2436
                     /locus_tag="OO609_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6635261.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIG"
BASE COUNT          558 a          588 c          683 g          607 t
ORIGIN      
        1 gatcatggcc agaaaacgca aatcaagaaa taacagtaaa attggtcacg gagcgatcag
       61 tcgcattggt agaccgaata atccttttga accgcgtcgc aatcgctacg cacaaaaata
      121 cttaacgttg gcgctaatgg gcggtgccgc ttttttcgta ttgaaaggtt gtagcgatag
      181 tagcgatgtc gataacgacg gcgacggaac attttacgcg acagtgcagg attgtattga
      241 tgacggtaat aatgccgaca tttgcgcgcg tggctggaac aacgccaaag cggcatttta
      301 tgccgatgtt ccaaagaata tgactcagca gaactgtcag tctaagtacg aaaattgcta
      361 ctacgacaat gttgaacaga gttggatccc ggtcgtttct ggatttttgt taagccgggt
      421 tattcgtaaa gatcgcgatg agccgttcgt ctataacagc ggcggatcct catttgcttc
      481 ccgccccgtc tggcgcaaca cttccggtga ttactcctgg cgcttcggtt ctggcaaaaa
      541 agagtcttac tcttcaggcg gctttaccac cagaaaagcg tcgaccgttt cgcgcggcgg
      601 ctatggtcgt tcttccagcg cccgtgggca ttggggaggc taatcatgct gagacacaac
      661 gttcctgtgc ggcgggatct ggaccagatc gccgctaaca acggtttcga ctttcatatc
      721 atcgacaatg aaatctattg ggacgagagt cgggcttacc gttttactct gcgccagatt
      781 gaagagcaga tcgaaaaacc gactgcggaa ctgcatcaga tgtgccttga ggtggtggat
      841 cgcgcggtta aagacgaaga gatcctgacg caactggcga tcccgccgtt gtactgggat
      901 gtgatcgccg aaagctggcg cgcacgcgat ccttcgctgt atggccggat ggattttgcc
      961 tggtgtggca atgcgccggt gaagctgctg gagtacaacg ccgatacgcc gacctcgttg
     1021 tatgagtcgg cttatttcca gtggttgtgg ctggaggatg cccggcgcag cggcattatt
     1081 ccgcgtgatg ccgatcagta caacgccatt caggaacgcc tgatttcgcg ctttagtgag
     1141 ctttacagtc gggaactgtt ttatttttgc tgctgtcagg acaccgatga agacaggagt
     1201 accgtgctgt acttgcagga ctgcgcccag caggcagggc aggagtcgcg gtttatctac
     1261 attgaagatc tcggtttggg cgtcggcggc gtactgaccg atcttgatga taatgtcatc
     1321 cagcgtgcat ttaagctgta tccgctggag tggatgatgc gtgacgataa cggtccgctg
     1381 ctgcgcaagc gtcgcgagca atgggtggag ccgttatgga aaagtatctt gagtaataaa
     1441 gggctaatgc cgctgctttg gcgcttcttc cctggtcatc ctaaccttct tgcatcctgg
     1501 tttgagggtg aaaaatcgca gattgccgct ggcgaaagct atgtgcgtaa accgatctac
     1561 tcgcgcgaag gcggaaacgt caccattttc gacggtcaga ataacgtcgt tgaccacgct
     1621 gatggtgatt acgccgatga accgatgatc taccaggcgt ttcaacctct gccgcgattt
     1681 ggcgatagct acacactcat cggtagctgg attgtcgatg atgaagcgtg cggaatgggg
     1741 atccgtgaag ataacacact gatcaccaaa gacacctcac gtttcgttcc gcattacatt
     1801 gctggataag aatgttttag caatctcttt ctgtcatgaa tccatggcag tgaccatact
     1861 aatggtgact gccattgatg gagggagaca cagtgcactg gcaaactcac accattttta
     1921 atcaacctat accattaaat aacagcaact tatacctgtc tgatggcgcg ctttgcgaag
     1981 cggtaacgcg tgaaggtgct ggctgggata gcgattttct agccagtatt ggtcagcagt
     2041 taggaacggc tgaatccctt gaactggggc ggctggcgaa tgtaaacccg cctgaactgt
     2101 tgcgctacga tgcccagggg cggcgtctgg acgatgttcg tttccatccc gcatggcacc
     2161 tgctgatgca ggcgttatgt accaatcggg tgcacaatct tgcctgggaa gaagacgctc
     2221 gctccggcgc atttgtggcg cgcgcggcgc gttttatgtt acatgcgcag gttgaggctg
     2281 ggtcgttatg tccagtaacg atgacctttg ccgccacgcc attgttgtta cagatgttac
     2341 ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat tctcacttat
     2401 tgccaggtgg gcaaaaacgc ggtttgttga ttggca
//
LOCUS       DAJTYH010000228         2362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTYH010000228 DAJTYH010000000
VERSION     DAJTYH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..545)
                     /locus_tag="OO609_004072"
     CDS             complement(<1..545)
                     /locus_tag="OO609_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635262.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO609_004073"
     CDS             complement(1207..2196)
                     /locus_tag="OO609_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635263.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          639 a          540 c          561 g          622 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gc
//
LOCUS       DAJTYH010000229         2300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTYH010000229 DAJTYH010000000
VERSION     DAJTYH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..230
                     /locus_tag="OO609_004074"
     CDS             <1..230
                     /locus_tag="OO609_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6635264.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(484..648)
                     /locus_tag="OO609_004075"
     CDS             complement(484..648)
                     /locus_tag="OO609_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635265.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1550..1834
                     /locus_tag="OO609_004076"
     CDS             1550..1834
                     /locus_tag="OO609_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6635266.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1822..>2300
                     /locus_tag="OO609_004077"
     CDS             1822..>2300
                     /locus_tag="OO609_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6635267.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     "
BASE COUNT          593 a          488 c          658 g          561 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 acgtacttct cggggcgctt accttcaaaa tgatggacat tatttttgtg gaaccggagg
      301 taacagacca gacggtttaa atgagccggt tactttaaac gggtgatgga cagcacctca
      361 aaagttcaga tggattatat ttgtctgcaa taccccgggt attttcgatt tgcgaaatgg
      421 atggaacgac ttgctcaggg gatagctgat ggagttactg aagtaccgaa ggaccactaa
      481 aaatcacagg ccgggaattt cggcgtttcc ggcgtaggtg tgccacgaat gcatccagtg
      541 gcgtgatgtt ggatgcaatg ctgtatgaga gatgaggggc ggcgtccaga cgcagccttc
      601 aaaccgcctt gagctgctgc cgttaagagc ggtggtggtg agcgtcaagg ttaaaaggcg
      661 cagaacatgt gtcaggtagg tgttgcggag atgaacgaaa gtgaactgtc gatgaagtgt
      721 cgaaaggaac ctctaggcga tgtcgaaaat gtgctatcta cgcagcgcat gacgagtcca
      781 gcagatatct ggtactggct gggcggcatc cggcatatag gcagcatgaa cgcaacacag
      841 gctctcgtac ggaacgtggg aacctgtcgt ctcgatgcta agggagcggc gcggagcaga
      901 ggcactgcaa gcgcctgagt accgatgcga gacacagggg cagatggctt cgtagtagtg
      961 aggaagtgcc tgtaatgggc atggagcgaa gggggtcagt cgttggggct ggtttatcgg
     1021 tcaaccggat agccgggagg agcccatgag tcagccaaag ccgttcgagg tctctaagta
     1081 tgcggtttgg aaagcatacc agcgggtcaa agctaatcga ggtgcggcag gtgtcgatgg
     1141 gcagtcagtc gaggcgtttg aggttaccaa tcgaagctat acccggtaat ggcgcaggtt
     1201 gacctgcact tggccaagtg gattgcccgc aaacacaagc gagcgcgagg tagtctggtg
     1261 cgtgcattcg agtggttact gcgaattcgt cacgactgcc cgacgctgtt tgcacactgg
     1321 tgcttagctt acgatacttg aatggttcaa cgacaagagc cgtatgacgc gagagtgtca
     1381 cgtacggctc cgtgggagcg taggggggcg gtgcccctgc gccacccgat atcccctttg
     1441 agtgcggtta cgtccacaga ggtaaatgcc gctgacgcgg catctgtaat ctgttacttt
     1501 gtgtatatca tttgctatat attgtcaata tgctattgtt gtaaagcaaa tgatatacgg
     1561 aggctttatg aaatcaggtg ttcagctcaa tctcagagca agagaatcac agcggatact
     1621 cattgatgct gctgcagaaa ttcttcataa gtcgcgtaca gatttcattc tggaaatggc
     1681 ctgtaaagct gctgaagatg ttattctcga ccggcgggtg tttaatttta atgacaggca
     1741 atatgaagag ttcattgaaa tgcttgatgc tccggttgca gatgatcctg ccatcgaaaa
     1801 attgctggca aggaaacctc aatgggatgt gtaactgcac cagaaccttt atcctcattt
     1861 catcaggtcg ctgaatttgt cagcagtgaa gctgtacttg atgactggtt aaagcagaag
     1921 gagctaaaaa atcaggcaat cggagccacc cggacttttg ttgtatgcag aaaaggtacg
     1981 caacaaatag tcggttttta ctcactggcc actggtagtg tcaatcatac agaagctaca
     2041 ggtaaccttc ggcgcaatat gccagacccc attcctgtaa ttatacttgc acgcctggct
     2101 gtagatgtgt catttcgagg aaaagggctt ggcgcggatt tacttcatga tgcagttcgt
     2161 cgttgttaca gggttgccga aaatatcggt gtgcgtgcaa ttatggttca tgcgcttacg
     2221 gaaaacgcaa aacagtttta tattcatcat ggttttaagc catcaaaaac tcaggtgcaa
     2281 acattgtttc ttaaactccc
//
LOCUS       DAJTYH010000230         2270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTYH010000230 DAJTYH010000000
VERSION     DAJTYH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            5..>2270
                     /locus_tag="OO609_004078"
     CDS             5..>2270
                     /locus_tag="OO609_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6635268.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGY"
BASE COUNT          599 a          504 c          571 g          596 t
ORIGIN      
        1 tattatgatg acgaccagaa tagtggttgg cctcacggca gggacgtgtc tgattttctc
       61 gcaaaacctg atggccgagg tcagtgtatt caatccggcg cttctggaaa tcaaccatca
      121 atccggagtc gatattcgcc agtttaatcg ggcaaacctg atgcccccag gtgtttatag
      181 cgttgatatt tttatcaacg gtaaaatgtt tgaacgtcag gatgtgacat ttgttcagga
      241 taatccagat gctgatctgc acgcttgctt tattgccatt aaaaaaacac tgtcctcctt
      301 tggcataaaa gttgatgcgc tcaaatcgtt caatgatgtg gatgagacgg tttgcctcga
      361 tcctgctcca cgtattgaag gctcatcctg gcagtttgac agtgataaat tgcagctgaa
      421 tatatccatt caccaaatct acatggacgc gatggcttat gattacatca gccccacgcg
      481 ttgggatgag gggattaatg cgctcaccat caactacgat ttttctggtt cacatacact
      541 acgttcagat tatggttcac aagagacaga taccagttat ctcaatctgc gcaatggact
      601 gaatattgga ccgtggcggc tacgtaatta cagtacttta aacaccagcg atggccgtgc
      661 ggaatacaac tccattagta cctggataca gcgcgatatt gccgcgttaa gaagccagat
      721 tatgattggt gatacgtgga cggcgagcga tattttcgac agtacgcaaa ttcgcggcgc
      781 gcgtttgtat actgataacg atatgctacc cgccagccag aatggctttg ctcctgtggt
      841 tcgtgggatt gcaaagtcca acgccaccgt catcattcgg cagaatggct acgtgattta
      901 tcagtcagcc gttccacaag gtgcttttga gatcaccgat ctcaacaccg caagtacagg
      961 tggcgatttg gacgtaacca tcaaagaaga agacggtagc gaacaacgat tcacccaacc
     1021 ttatgcttca ttggcgattc ttaaacgtga aggtctgaca gatgttgatg tcagcgtggg
     1081 tgaattgcgc gatgaagacg gatttacacc ggacgtcctt caggcgcaaa tacttcatgg
     1141 tttttcccac gggatcactt tatatggagg tatgcaggct gctgaaaatt atggttctgc
     1201 agctctgggt gtcggtaaag atcttggcgc tttgggcgca atttctttcg atgtgacaca
     1261 tgctcgtgcg aattttagcc atgatgatac agaaacgggt cagtcatatc gctttctcta
     1321 ttcaaaacta tttgacgaca cagacactag cttgcgcctg gttggctatc gttactccac
     1381 cgagggctac tataccctca atgagtgggc atcgcggcgc aacagccctg aagacttttg
     1441 ggaaacaggt aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt tggggagaga
     1501 ttatggcaat ttatacctga cattaagccg gcaacaatac tggcataccg atgatgtcga
     1561 acgattaatg caatttggct acagcagtag ctggaagcgt ctctcgtgga acgtctcctg
     1621 gagttattcc aatactgcca gacaggggac ggggaacaac catgccagtg ataacaccag
     1681 tgagcagatc tacatgctct ctttatctgt tcctttatcg ggctggtggg gtaatagtta
     1741 cgccacctat tctgtttcgc aaaacgataa ttccggtagc tcacatcaac tcggactcag
     1801 cggtacggcg ctggaaagaa ataacctttc atggaattta atgcagtcct ataacagtca
     1861 tgatgatgag gttggcggta atatgtccct gacctatgat ggctcttatg gcacggtgaa
     1921 cggcagctat aactacagcc aaaattccca gaggctgaat tatggtatca gagggggaat
     1981 tctggcacac agcgaagggg taacgttaag tcaggagtta ggtgaaacta ttgctcttgt
     2041 taaagcacct ggggccgcca ggttagaaat agataatatg cgcggtgctg cgacggactg
     2101 gcgcggctat acggtcaaga cacagctaaa cccttatgat gaaaatcggg tagcaatcag
     2161 cgataactat ttctcgaagt cgaatataga acttgataat accgtcgtta cgatggttcc
     2221 cacgcgtggt gcagtggtta aagcggagtt tgtgactcat gtgggttatc
//
LOCUS       DAJTYH010000231         2238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTYH010000231 DAJTYH010000000
VERSION     DAJTYH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="OO609_004079"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="OO609_004079"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6635269.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          553 c          659 g          454 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac gccctgcc
//
LOCUS       DAJTYH010000232         2196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTYH010000232 DAJTYH010000000
VERSION     DAJTYH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(110..325)
                     /locus_tag="OO609_004080"
     CDS             complement(110..325)
                     /locus_tag="OO609_004080"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635270.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            461..592
                     /gene="yqgB"
                     /locus_tag="OO609_004081"
     CDS             461..592
                     /gene="yqgB"
                     /locus_tag="OO609_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6635271.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            601..>2196
                     /gene="speA"
                     /locus_tag="OO609_004082"
     CDS             601..>2196
                     /gene="speA"
                     /locus_tag="OO609_004082"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6635272.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDG"
BASE COUNT          496 a          554 c          605 g          541 t
ORIGIN      
        1 tcattctgtt aaaaacgtgg ctggaaattt ttcctgacaa tgccggcatt ctgcgtattt
       61 atcttttgca attttctgcc attgtggggt ataaaacgcg gcgcgcggct taaataaaaa
      121 gcacacgacg tttctttcgt gttgccactt ccagccgggt tgaaatcaga gttttggctt
      181 gtgggttcgt cttaacaggc ggccgtggag gtgatacgaa ataatgaacc gttgtctgct
      241 gcttaacctg tctcaccgtt ctggtgaaga ctcgttcccc gcactctgca tctctgcttt
      301 gcatacctgc cgatgttata cccatctcgg cgcttctcag gattcaagag ctggttacag
      361 ttactgagga ctgaacaagg gcgctcttgt aaaaacaaga gttttctcgt ggtttcgccg
      421 aactgtcaca cttacgttcg gttatgtgct taataatgtt atgaaaaaga aaccggttgc
      481 gcagtcggag cgtcagcata cactgctgga aaatccatgt gcttatgggt tgttatcgca
      541 gttccaggct gcgacagtcg ttaactgttt tacacttaat aaaataattt gaggttcgct
      601 atgtctgacg acatgtctat gggtttgcct tcgtcagcgg gcgaacacgg tgtactacgc
      661 tccatgcagg aggttgcaat gagctcccag gaagccagca agatgctgcg tacttacaat
      721 attgcctggt ggggcaataa ctactatgac gttaacgagc tgggccacat cagcgtgtgc
      781 ccggacccgg acgtcccgga agctcgcgtc gatctcgcgc agttagtgaa aactcgtgaa
      841 gcacagggtc agcgtctgcc tgcactgttc tgtttcccac agatcctgca gcaccgtttg
      901 cgttccatta acgccgcgtt caaacgtgcg agggaatcct acggctataa cggcgattac
      961 ttccttgttt atccgatcaa agttaaccag caccgccgcg tgatcgagtc cctgattcat
     1021 tcgggtgaac cgctgggtct ggaagccggt tccaaagccg agttgatggc agtgctggca
     1081 catgctggca tgactcgtag cgtcatcgtc tgcaacggtt ataaagaccg cgaatatatc
     1141 cgcctggcat taattggcga gaagatgggg cacaaggtct atctggtcat tgagaagatg
     1201 tcagaaatcg ccattgtgct ggatgaagca gaacgtctga atgtcgttcc tcgtctgggc
     1261 gtgcgtgcac gtctggcttc gcagggctcg ggtaaatggc agtcctccgg cggggaaaaa
     1321 tcgaagttcg gcctggctgc gactcaggta ctgcaactgg ttgaaaccct gcgtgaagcc
     1381 gggcgtctcg acagcctgca actactgcac ttccacctcg gttcgcagat ggcgaatatt
     1441 cgcgatatcg cgacaggcgt tcgtgaatcc gcgcgtttct atgtggaact gcacaagctg
     1501 ggcgtcaata ttcagtgctt cgacgtcggc ggcggtctgg gcgtggatta tgaaggcacc
     1561 cgttcgcagt ctgactgctc cgtaaactat ggcctcaatg aatacgccaa caacatcatc
     1621 tgggcgattg gcgatgcgtg tgaagaaaat ggtctgccgc atccgacggt aatcaccgaa
     1681 tcgggccgtg cggtgactgc gcaccatacc gtgctggtgt cgaatatcat cggcgtggaa
     1741 cgtaacgaat atacggtgcc gaccgcgccc gtagaagatg cgccgcgtgc gctgcaaagc
     1801 atgtgggaaa cctggcagga aatgcacgaa ccgggaactc gccgttcttt gcgtgaatgg
     1861 ttacacgaca gccagatgga tctgcacgac attcatatcg gctactcttc cggcaccttt
     1921 agcctgcaag aacgtgcatg ggcagaacaa ctctatctga gcatgtgcca tgaagtgcag
     1981 aagcaactgg atccgcaaaa ccgtgcgcat cgtccgataa tcgacgaact gcaggaacgt
     2041 atggcggaca aaatgtacgt caatttctcg ctgttccagt caatgccgga cgcatggggg
     2101 atcgaccagt tgttcccggt tctaccgctg gaagggctgg atcaagtgcc ggaacgtcgc
     2161 gctgtgctgc tggatattac ctgtgactct gacggt
//
LOCUS       DAJTYH010000233         2181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTYH010000233 DAJTYH010000000
VERSION     DAJTYH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..811)
                     /locus_tag="OO609_004083"
     CDS             complement(<1..811)
                     /locus_tag="OO609_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6635273.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPD"
     gene            complement(1410..1658)
                     /locus_tag="OO609_004084"
     CDS             complement(1410..1658)
                     /locus_tag="OO609_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6635274.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2013..>2181)
                     /locus_tag="OO609_004085"
     CDS             complement(2013..>2181)
                     /locus_tag="OO609_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6635275.1"
                     /translation="GVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIAR
                     VRSVEEILHAL"
BASE COUNT          568 a          386 c          526 g          701 t
ORIGIN      
        1 agtccggcga agatgttaat ctttgcaggg attgcagagc atgggatgac aatgggttat
       61 cactaatatc tattgaacat tcattcctca gattaagaat gctttccggg atatgactta
      121 tctgatttat gtcaaaataa ccgttcctta aattttccgg caggtatgga atatcggtta
      181 acatattgtc gattgcccat atggagtgaa tgttgtgagg tagagggggg agttcactaa
      241 gaagattccc tattgcgcta atctcttgta aagaaaatgg taaaggtggt aaaactgcca
      301 gcccattaca tgatacatct aatgtttcca gtagttctgg taaaacagga agagaacata
      361 attgattatt tgagacatga agctccttca aagatatggg gagtgtgggt agtgtgatta
      421 gttgattgtg ggacgcattc aataatttaa gtccttgagg caaagcaggc agttcaataa
      481 gtctgttata gctgacatta agttgtgtaa gggacgcagg caatggggag attaagctta
      541 aattattttt acttatatta attgatttaa ttcccggggg gatttcaggt aatgttgtca
      601 ggcctaattc agacaagtcc aggttcgtct cttggttttg tagacatgat actagtcgct
      661 gaaaagcgat gtctcgttgt tcttcttgta tgcggttatt tttccattca gtccaatggg
      721 ttagataatt ttcatatgcg cggctagtgt cgattgaata agtggagaac gaatttgaaa
      781 ttaatctgtg attgttattt gtcgggagca taaatatcag ggtgtattct ttatgttgag
      841 aggtgttgga ttctttttta tctgctttgc aatgttattg gttcccttca gcagtagaca
      901 ggaacttctg gaggaggcgg atatatagtt tgtgtaagca tattaactcc atatgttata
      961 tattgaagaa cttctgcttt acactatcct aaactggatc ggtttaatgt aaaaaaacga
     1021 tactttttgg gggggggcaa tttttcctta aggtatgaaa aaaggagctg aagctatata
     1081 atagctatta atgccactgg tatcagtgaa tcatgtatat aaaaaactgt gttaaggagg
     1141 ctgtccatta gcggtttttt attcttctgg aaccgcggca tctttgcgta tctttttgtc
     1201 ttctcaccgt ctggtcggtg ttttgctgag actgctaatt tcctgtcttt gctagtgctg
     1261 tactggtact gcccaatcat gattggcggg ggcgggcgga gttgagaccg cagacacgtc
     1321 gtatgcaaga acgtgctgcg gctggctggt gaacttccga tagtgcgagt attgaatgat
     1381 ttccagccgt tactgatttt acgtgctaat tagtgaacaa accactcgtc agcagactcc
     1441 caggtatctt tcagagtttc ctgaacaaaa gttttagctg aatctttatc ggcggtgcgc
     1501 gtaacagaaa ggccatcgtt gctggtggct tttacgatca cctctacatc gtcataacgt
     1561 ttactgatgc gtcgggttaa ttcttccttt aacgcatcca cagcaccggt tggcatttta
     1621 gtcattttct ctttggctat gcagatttca aaacgcataa aaatccctct gcactgtgtt
     1681 tttatacagt attattttta actgtatgga taaacagtgt caagagtgag ggaaagtgat
     1741 tttttggggc atgcatgggg caaaaatatt tgttgtgggg cattttgggg catgattggg
     1801 acactttgtt acatatgaac tttgccgatt ttcatatgac atggcaatcc aacgctttga
     1861 ttattctaaa gaaaacacat gctcttgggc gttctttagt gatttttaaa aatcccgtat
     1921 cacgcagtta aagtggcggg catactcttc aaggctggtg atccccaacc gcacccattt
     1981 aggatgtgac cattgcggta gaccgatgta aatcataacg cgtgaaggat ctcttccacg
     2041 ctacgcacac gggcgatgcg cgggtagata tgattaatgc tgttattgtg ttgctcggcg
     2101 ctggcggcac tacaggcgtc ttcggcaatc accagattaa agccaagttc ccaggcattg
     2161 cgggcggtgg attcaacacc g
//
LOCUS       DAJTYH010000234         2172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTYH010000234 DAJTYH010000000
VERSION     DAJTYH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..774
                     /gene="proB"
                     /locus_tag="OO609_004086"
     CDS             <1..774
                     /gene="proB"
                     /locus_tag="OO609_004086"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6635276.1"
                     /translation="RERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSA
                     LAAILAGADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTG
                     GMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIF
                     GAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVS
                     RYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            786..2039
                     /gene="proA"
                     /locus_tag="OO609_004087"
     CDS             786..2039
                     /gene="proA"
                     /locus_tag="OO609_004087"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6635277.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          516 a          550 c          628 g          478 t
ORIGIN      
        1 cgtgaacgct tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt
       61 ccggtaatca atgagaacga tgctgtcgct acggcagaga ttaaggtcgg cgataacgat
      121 aacctttctg cgctggcggc gattctggcg ggtgccgata aactgttgct gctgaccgat
      181 caaaaaggtt tgtataccgc tgatccgcgc agaaatccgc aggcagaact gattaaagat
      241 gtttacggca ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga
      301 actggcggca tgagtactaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac
      361 accattattg ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc
      421 gtcggtacgc tgttccatgc ccaggcgact ccgcttgaaa accgtaaacg ctggattttc
      481 ggtgcgccgc cggcgggtga aatcacggta gatgaagggg caactgccgc cattctggaa
      541 cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt
      601 gaagtcatcc gcatttgcaa cctcgaaggt cgcgatatcg cccacggtgt cagtcgttac
      661 aacagcgatg cattacgccg tattgccgga caccactcgc aagaaattga tgcaatactg
      721 ggatatgaat acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaggagct
      781 ggccgatgct ggaacaaatg ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac
      841 tctccagccg cgaaaaaaat cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa
      901 gcgaaatcat cctcaacgct aacgcccagg atgttgctga cgcgcgagcc aatggcctta
      961 gcgaagcgat gcttgaccgt ctggcactga cgcccgcacg gctgaaaggc attgccgacg
     1021 atgtacgtca ggtgtgcaac ctcgccgatc cggtggggca ggtaatcgat ggcggcgtac
     1081 tggacagcgg cctgcgtctt gagcgtcgtc gcgtaccgct gggggttatt ggcgtgattt
     1141 atgaagcgcg cccgaacgtg acggttgatg tcgcttcgct gtgcctgaaa accggtaatg
     1201 cggtgatcct gcgtggtggt aaagaaacct gtcgcactaa cgcggcaacg gtggcggtga
     1261 ttcaggacgc cctgaaatcc tgtggcttac cggcgggtgc cgtgcaggcg attgataatc
     1321 ctgaccgtgc gctggtcagt gaaatgctgc gtatggataa atacatcgac atgctgatcc
     1381 cgcgtggtgg cgctggtttg cataaactgt gccgtgaaca gtcgacaatc ccggtgatca
     1441 caggtggtat aggcgtatgc catatttacg ttgatgaaag tgcagagatc gctgaagcat
     1501 taaaagtgat cgtcaacgcg aaaactcagc gtccgagcac atgtaatacg gttgaaacgt
     1561 tgctggtgaa taaaaacatc gccgatagct tcctgcccgc attaagcaaa caaatggcgg
     1621 aaagcggcgt gacattacac gcagatgcag ctgcgctgac acagttgcag gcaggccctg
     1681 cgaaggtggt ggcggttaaa gccgaagagt atgacgatga gtttctgtca ttagatttga
     1741 acgtcaaaat cgtcagcgat cttgacgatg ccatcgccca tattcgtgaa cacggcacac
     1801 aacactccga tgcgatcctg acccgcgata tgcgcaacgc ccagcgtttt gttaacgaag
     1861 tggattcgtc cgctgtttac gttaacgcct ctacgcgttt taccgacggc ggccagtttg
     1921 gtctgggtgc ggaagtggcg gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc
     1981 tggaagcact gaccacttac aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa
     2041 taaaaccggg tgatgcaaaa gtagccattt gattcacaag gccattgacg catcgcccgg
     2101 ttagttttaa ccttgtccac cgtgattcac gttcgtgaac atgtcctttc agggccgata
     2161 tagctcagtt gg
//
LOCUS       DAJTYH010000235         2121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTYH010000235 DAJTYH010000000
VERSION     DAJTYH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            29..1426
                     /gene="ccp"
                     /locus_tag="OO609_004088"
     CDS             29..1426
                     /gene="ccp"
                     /locus_tag="OO609_004088"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6635278.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1637..>2121
                     /locus_tag="OO609_004089"
     CDS             1637..>2121
                     /locus_tag="OO609_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6635279.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
BASE COUNT          577 a          445 c          549 g          550 t
ORIGIN      
        1 aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta ccgcgatcgg
       61 cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt accacgataa
      121 taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata ataaggtttt
      181 aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag aattacccgc
      241 ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc ttggatataa
      301 atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg tttcgcaaag
      361 cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa cgcgttatac
      421 cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac tggcctggat
      481 tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc atcgcaatga
      541 accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg cgttgggctt
      601 tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg ctcattgcca
      661 tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg gtggcgcagt
      721 tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc agttctggga
      781 tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc cgattgaaat
      841 ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc agcttaaagc
      901 gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg atgccattgc
      961 tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt tgcgtggaga
     1021 tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag ataataaatg
     1081 tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt tggggctgaa
     1141 aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga atgtgactaa
     1201 agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg ctttaacggc
     1261 accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac tgatgctgcg
     1321 ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag ctttcctgca
     1381 cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa tttgatcgcc
     1441 cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt aatattatca
     1501 tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt atttaaatta
     1561 agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc cttcaaataa
     1621 atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag aactactcga
     1681 ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat ttccgctgca
     1741 cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc ttgatggcaa
     1801 cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg tccataaatt
     1861 gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc aatccgcagc
     1921 catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg cgccgaaaaa
     1981 tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc tcggcgggat
     2041 ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa cggataaacc
     2101 aaacctggtg tgcggtccgg t
//
LOCUS       DAJTYH010000236         2109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTYH010000236 DAJTYH010000000
VERSION     DAJTYH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..234
                     /locus_tag="OO609_004090"
                     /pseudo
     CDS             1..234
                     /locus_tag="OO609_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OO609_004091"
     CDS             655..1233
                     /locus_tag="OO609_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635280.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="OO609_004092"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="OO609_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..2109
                     /locus_tag="OO609_004093"
                     /pseudo
     CDS             1584..2109
                     /locus_tag="OO609_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          680 a          385 c          520 g          524 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggaaggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatga catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag cagaccatgg agcgaggaaa gacgcaacga ctggcagaga tggctgcggc
     1921 caacagtgac gtccccatga tgaagaatgt ggctaaaacg atagggaaaa ggttgtacgg
     1981 tatcctgaac gccatgcgcc atggcgtatc aaacggaaat gcggaagcac tgaacagcaa
     2041 gatcaggctg ctgagaataa aagccaaggg ctaccgcaac cgggagcgct ttaagctggg
     2101 agtgatgtt
//
LOCUS       DAJTYH010000237         2109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTYH010000237 DAJTYH010000000
VERSION     DAJTYH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..1550)
                     /locus_tag="OO609_004094"
                     /pseudo
     CDS             complement(1..1550)
                     /locus_tag="OO609_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(1614..>2109)
                     /locus_tag="OO609_004095"
     CDS             complement(1614..>2109)
                     /locus_tag="OO609_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6635281.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
BASE COUNT          446 a          635 c          520 g          508 t
ORIGIN      
        1 cccgtcggag cgacatcatc agcggtggct gtgtagggga ctgcctggtc cctgtcgtca
       61 aaaaaaacgt cacagtctac tgcgccactt tcggtataac ggggattaat gattttttta
      121 atttccacgg tgcattcctc acgatgtgcg aataaaaagc ccgggcattg cgccagagac
      181 atgtgcatcc ggcaccccgg acagggcgca atatgacccc ggtaatgaat gctctgaaca
      241 tcccgtaatg aaaaattgtg gggatgctat atacgttccg gtgggagtac cgggcactga
      301 aatccccacc ggtcccagtc gtgagcctct gtatgactgc cccctgacaa gtctgatgac
      361 tttatcaccg tcagcttctc cctggtacgc agcaataatc agcccgccaa tgtcagggtc
      421 tccccatctg ttgcggacag agctcgccac gattctgtaa ataatatctt ctgtggttat
      481 atttattttc acccagtcag tctggatatg ggccagtagc ggtagcgggt gtgataaatg
      541 gggccgttaa tgccgtaaaa agtaagggat ttggctctgt accgcggttc tgttgtctca
      601 gggcgtgcat cagtccaccg gatgctgagc accccttcaa accgtgtgtc gggtatgatg
      661 atgccgtagg ggccagcaac ggaatattca cctggcagcg cattccttac ccaggccagg
      721 aaatcactct tagtgtcaaa acggataaca tcttcaggca gaaaagcaca cccaaagccg
      781 aatgcgccgg gtatcgccag acggcctttt gtccggtcgt aaatgtcgct ctgtgcttcc
      841 atcgtggccg cacttttcag ccccagatta tcccgggact tctgttgtgc cttttcgcct
      901 gctgctgcga tttctgacag atggttagcc attttcagag tgccggtcag cgcagcatca
      961 atgtcatttt tggcctgttc tgctgcgcgg gcataacctg cggcatcctg tgcgctctgt
     1021 ccggctgcgg ctgcatttgc tcctgtgctt gtcacatctt ccgcagtggc ctttctgtcc
     1081 tcagctgtga cggttgcatc ctgtcgggct ttctccgcaa aacgttgtgc atcatcacgg
     1141 gccgttgcgg ctgccgccac atcctgcgcc gtctgctgtg cgtttccggc tgcagccccg
     1201 gcactctgct gcgcctgcgc caccatttcc tcaaagcgct tcatcacctc cggacgtaaa
     1261 tcaccatcct ttggtgcatt cagaaacgcg ttcagtgtcc ccggtgcatc agtcggtgcc
     1321 acatcaatat ccccgacacg ggttggctgc cagccgttac agtgaagcgc aacctcgtaa
     1381 taccccggct cagcctcaat cacataagca ccgttattgt ccgtcacgca agtggcaaca
     1441 acgtgtgcca caacggtcgg actggttctt ctggcccgca gttcaatcgc acaatttacg
     1501 acaggtttac ccgccccatc tttcagtaca cctgaaatct ttactgccat attcacccca
     1561 caaaaagccc gcctgaacca gcgggctgtc ataacactgt gttacctggc taatcagaac
     1621 ttataaccga cacccacgat gaaaccgtca gtgcgccagt cgccactgcc ggaaccttca
     1681 taagcaaggt caatggccac ggattcggtc gggttaaact gcacgccagc cccccacgcc
     1741 agagacgtgt tgctgtggcg gttgtcatca ctcccggtca gcacatcgtg cgttttcccc
     1801 ttgttgtcag ttacgcggag ataatccccg gagaaggtcg acacacggct gtaagccacg
     1861 cccgccatcg catacgcgct gaaccattca ttcacgcgca cagacagccc cgccatcacg
     1921 ctgaaccagc ggttacgcac ggaatcctca tgccagcggg tatcactgta atgcgttttt
     1981 tgctcatctt cagcgttggc atagctgaat gacgtcacca gccccagcgt gtccgtaaac
     2041 tcataacggt atttcacgtt aatcccgttc agatcatcac tgccgggagc gttcgtcgag
     2101 gcatgaaga
//
LOCUS       DAJTYH010000238         2092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTYH010000238 DAJTYH010000000
VERSION     DAJTYH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..799
                     /locus_tag="OO609_004096"
                     /pseudo
     CDS             1..799
                     /locus_tag="OO609_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OO609_004097"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OO609_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="OO609_004098"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="OO609_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          506 c          632 g          438 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc ctggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc tt
//
LOCUS       DAJTYH010000239         2086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTYH010000239 DAJTYH010000000
VERSION     DAJTYH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..1300
                     /gene="mppA"
                     /locus_tag="OO609_004099"
     CDS             <1..1300
                     /gene="mppA"
                     /locus_tag="OO609_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6635282.1"
                     /translation="DGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDG
                     KATPDQLGVTAVDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLI
                     GNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDIT
                     ESFPKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMT
                     EKVLGTGEKPAWHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKL
                     TLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGD
                     YNEPSTFLTLLTSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQA
                     PIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1351..>2086)
                     /gene="ynaI"
                     /locus_tag="OO609_004100"
     CDS             complement(1351..>2086)
                     /gene="ynaI"
                     /locus_tag="OO609_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6635283.1"
                     /translation="QFEKQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLS
                     GLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGW
                     RITKITTFDNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVR
                     EMLKNHPAIDQRQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIV
                     QSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          602 a          482 c          494 g          508 t
ORIGIN      
        1 ggatggcaca ccggtaacgg cgcaagattt tgtctacagc tggcaacgtc tggtggaccc
       61 aaaaacattg tcgccatttg catggtttgc cgcgctggcg ggaatcaaca acgcacaggc
      121 gattattgat ggtaaagcta cgcctgatca gcttggcgtc accgcagttg atgcccatac
      181 tttgaaaatt cagcttgata aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc
      241 cttcttcccg gtgcaaaaag ccaacgtaga aagcggtaaa gagtggacga aacccggaaa
      301 tctgatcggc aatggcgctt atgttcttaa agagcgcgta gtcaatgaaa aactggtcgt
      361 ggtaccgaat acccattatt gggataacgc caaaacggtg ctgcaaaaag tgaccttcct
      421 gccaattaat caggaatccg cagccactaa gcgttacctc gcgggggata ttgatatcac
      481 cgaatccttc ccgaaaaata tgtatcagaa gctgttgaag gatattccgg ggcaggttta
      541 tacgccaccg cagctcggga cctattatta tgcctttaac acgcaaaaag ggccgacggc
      601 agatcagcgc gttcgcctgg cattaagtat gacgatagat cgccgcctga tgaccgaaaa
      661 agtgttgggg acgggcgaaa agccagcgtg gcattttata ccagatgtta ccgccggatt
      721 tacgccggaa ccttcgccgt ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa
      781 aactttgttg agcgcagctg gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta
      841 taacacttca gaaaaccatc aaaaaattgc gattgctgta gcatcgatgt ggaaaaagaa
      901 ccttggcgta gatgttaaat tgcaaaatca ggaatggaaa acctatatcg atagccgtaa
      961 caccggcaat tttgatgtta tccgcgcctc atgggtgggg gattataatg aaccctccac
     1021 tttcctgaca ttattaacgt caatgcattc aggaaatatt tcacgcttta acaatccggc
     1081 atacgacaaa gttctggccc aggcatcgac ggaaaatacc gttaaagcgc gtaatgccga
     1141 ttacaacgcg gcagaaaaaa tcctcatgga gcaagcaccg attgcaccaa tttatcaata
     1201 taccaatgga cgattaatca aaccgtggct gaaaggttat cccattaata accctgaaga
     1261 tgtagcgtac agtcggacta tgtatattgt gaagcattga tgtgatggga actggcgtta
     1321 cccttgtgga taacgccagt gatgtctgat ttagcgcccc tgttccggcg gtgtaatgtt
     1381 atccatatac agcgtctggc tggggaaggc aaagtccgcg ccgtgtgact gtacaatatc
     1441 gataatcttc aaataaacgt cttgctgtgc agcaagccac tcagcccata ccgtggtttt
     1501 ggtaaagcaa taaaccataa tattcaatga cgagtcagca aactggttga aataaaccag
     1561 taaggtttgt cgctggtcga tggccgggtg atttttcagc atctcacgta cggcttcgac
     1621 aataacgccc acttttgccg catcctcata acgtaaacca atggtcgtgg taatgcggcg
     1681 gttggtcatt cgtcctgggt tttctacgct gatcgacgaa aacagcgagt tcggtacgta
     1741 caatggacga ttatcaaagg tcgtaatttt ggtaattcgc cagccaattt ccgctactgt
     1801 accttcgata tttctgtccg gtgaacggat ccagtcgcca atactgaaag gacggtcgaa
     1861 atagagcatg atcccggaaa agaaattact cagaatatct ttaccggcca taccgacagc
     1921 cagaccacca ataccaccaa aggtcagcaa gccagaaagg ctcatgccga aatgttcgcc
     1981 ataaagcaga acaagcacta caataatggt gattttgata atgcgcgaca taatccgcgc
     2041 actggtgata tcgcgacctt ttttaatctg ctgtttttca aactga
//
LOCUS       DAJTYH010000240         2048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTYH010000240 DAJTYH010000000
VERSION     DAJTYH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            711..1862
                     /locus_tag="OO609_004101"
                     /pseudo
     CDS             711..1862
                     /locus_tag="OO609_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          566 a          452 c          517 g          513 t
ORIGIN      
        1 ctttttatta tttcctgcaa tggcgattac ggctattgac agtaatgaag ttattagtga
       61 accaagacct aaagtagttc cgggaccaac accgttgtcg cttacggtta ctgacaggaa
      121 actgattgta ccagcgctta ttgtttgagt aaaacctgta atagatgcgg caaatcctgt
      181 ttttgaacca tttatttgta atgcgataaa attggctatt gggtaataaa aaccataggt
      241 tggataattt ttcaatcttt gataattgat ttccgattat gaaaaataaa actgagaagg
      301 agaaaataat tgagtaacta aaagtactca ggccataagt atgcatgaaa attgatggtg
      361 atattgatat gtagcaaaat agagaactga gtattaatga gacaagtata gtgccgcaga
      421 taaactcttt ggtcacaaag gatgcactgt tttgacatat tactttctca tttgcatgag
      481 ttatctttgg tgataataac attattataa tactgaacag catcataaca agaaatataa
      541 cattccagtt ataatattca ataatcaatg cgcgaggtgc tactaatggt attactgcgc
      601 ttacagatga tatatcagaa agcgcccgtt ttagttttaa tggagggtga tcttacccac
      661 caatagtgga cacaggacta agtgagtaaa ctctcaacca gaggtgactc atgacaaaac
      721 cagtatcaat cagcaagaag ccccgtaaac aacatacgcc tgaatttcgt aacgaagccc
      781 tgaaactcgc tgaacgcatc ggtgtggccg ccgcagcccg tgaactcagc ctgtatgaat
      841 ctcagcttta tgcctggcgc agtaaacagc agcaacaaat gagttcgtca gagcgcgaaa
      901 gcgaactggc cgctgaaaat gtccgcctta aacgacaact ggcggagcag gctgaggaac
      961 tgtccatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga agtatgtctt
     1021 tatcgaaaat catcgggcag agttcagcat caaagcgatg tgtcgtgtac ttcgggttgc
     1081 ccgcagcggc tggtatgtct ggctcaggcg tcgtcaccag atgagcctgc gccaacagtt
     1141 tcggctcacc tgcgatgccg ctgtccataa ggcattcttt gaggcaaagc agcgatacgg
     1201 tgctccccgc cttgctgacg aactgccgga gttcaatatt aaaaccattg ccgccagcct
     1261 gcgtcgtcag gggctgcggg cgaaagccag ccggaagttc agcccggtca gctaccgtgc
     1321 acatggcctg cccgtattgg agaatctgct ggagcaggac ttcagcgcca gtggcccgaa
     1381 accagaagtg ggcgggtgac atcacgtact tgcgtaccga tgagggctgg ttgtatctcg
     1441 cagtagtcat cgacctgtgg tcacgcgccg tcattggctg gtcgatgtca ccgcgaatga
     1501 cagcacaact ggcctgtgat gcactgcaaa tggcgttgtg gcggagaaga cgcccggaaa
     1561 gcgtcattgt tcatacggac cgtggtggtc aatactgttc aggggattat caggcaatgc
     1621 tgaagcgaca caacctgcgt ggcagtatga gtgcgaaagg ctactgttat gacaatgcct
     1681 gtgtggaaag cttctttcat tcgctgaagg tggaatgtat ccacggggaa cgctttagca
     1741 gccgggaaat aatgcgggca acggtgttta attatatcga gtgtgattac aatcgctggc
     1801 gtcgtcacag tgcctgtggc ggtctcagcc cggaacaatt tgaaaaccat aatctcgctt
     1861 agggccgtgt ccacattacg tgggtaggat cagtatgggt cgtcttgtat ttgtcatact
     1921 gcccaatctc accaatcgaa aatgctcctt ttggcaaagg ttcaagcagg tctctgggaa
     1981 tggaggtcgc tggcgtcgga tataaaaaac cctcacggct tagcaaggct ggcgtcacta
     2041 tcggtgta
//
LOCUS       DAJTYH010000241         2043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTYH010000241 DAJTYH010000000
VERSION     DAJTYH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..435)
                     /locus_tag="OO609_004102"
     CDS             complement(<1..435)
                     /locus_tag="OO609_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HCR6635284.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASAL"
     gene            complement(438..998)
                     /locus_tag="OO609_004103"
     CDS             complement(438..998)
                     /locus_tag="OO609_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6635285.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(1124..1474)
                     /locus_tag="OO609_004104"
     CDS             complement(1124..1474)
                     /locus_tag="OO609_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6635286.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(1677..>2043)
                     /gene="intI1"
                     /locus_tag="OO609_004105"
     CDS             complement(1677..>2043)
                     /gene="intI1"
                     /locus_tag="OO609_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6635287.1"
                     /translation="ERKYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRA
                     FKRAVEQAGITKPATPHTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKV
                     GGAGVRSPLDALPPLTSER"
BASE COUNT          404 a          609 c          616 g          414 t
ORIGIN      
        1 caaggcgctg gccagcacga tgcctttgtc ggtttgcatc gccagctcgg tcagcatctg
       61 gacggcctgc cggtaatggc tcatggtgaa gggccggaaa ccgaacacgg tttgcagctc
      121 gctcaggtgc tcgcgccggg tctgctcccg ctggccgtac tcgttccagc tttcgacgcc
      181 gaccttgagc tggtcggcga ccagcttcaa caagggcggg aacggtagtt catcgacgcc
      241 caggatgacg ccgggaaagc gcaggtaaca gagctgcacc gcgaagccca gccgattggc
      301 tggcccgcgc cgctgtcgga tgatcgagag gtcggtatcg ttgaatgtgt aatgtcggat
      361 caggtcgtcc ttggagtccg gcaacgccag caggctttcc cgctcggcgg cggacaggat
      421 ggaacgacgt ggcatattta ctgatccgtt ctcaagtatt gatacagggt ttcgcgactg
      481 attccgaatt cacgagcaag cttggtcttt tgctcgccag cctcgacacg ttggcgcagt
      541 tcggcaatac gctcagacga cagggatttc ttcctgccac ggtaagcccc gcgttgcttg
      601 gcgagcgcaa taccctcgcg ctgacgctcg cggatcaggg cgcgctcgaa ctcggcgaac
      661 gcgcccatca ccgagagcat caggttcgcc atcggagagt cttcgccagt aaaactgagg
      721 tgttccttga cgaattcgat atgcacgccg cgttgtgtca gcgtttgcac gatccggcgc
      781 aaatcatcga gattgcgcgc caggcgatcc atgctatgca ccaccacggt gtcgccggtg
      841 cgggcgaagc ttatcagcgc ttccagttgc ggacgcttga catccttgcc ggatgccttg
      901 tcgctaaaag cgcgatcaac cttgacgcct tccagttgcc gttccgggtt ctggtcgaag
      961 gtgctgaccc tgatataccc aatgcgctgt ccagtcatgg aattccctgc aaaatgtcag
     1021 ggaagactct atgaccttca acgagatatg tcaataaatt caaaattcaa tcctatcctg
     1081 acgcaattta cacatggcat ctgacatcag gttagggtat gcctcaacct gacggcggcg
     1141 aatcacaagc gtccggtttg acgctggttt cggtcgcagt gctggcccgc gcctggaagc
     1201 gctgatagca ctccagcccg cagaaatgct ccacgtactc ggccccttcc ggcgtgaagg
     1261 cggcatcgag cgggatttcc ttgcagcaca cgcagcaact ggtgctcggt tcattggcgt
     1321 tcatggtggt gttcctccat tggttgacga agccgacgaa ggccgccgcc ggcatcggcc
     1381 tggcgaacag gaaaccctgc accgtgtcgc aacccgcctg ccgcaaccac gcaaggcagt
     1441 cgggtgtttc cacaccctcg gctaccacct ccattgtcgt tttcagaaga cggctgcact
     1501 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatccatca ggcaacgacg
     1561 ggctgctgcc ggccatcagc ggacgcaggg aggactttcc gcaaccggcc gttcgatgcg
     1621 gcaccgatgg ccttcgcgca ggggtagtga atccgccagg attgacttgc gctgccctac
     1681 ctctcactag tgaggggcgg cagcgcatca agcggtgagc gcactccggc accgccaact
     1741 ttcagcacat gcgtgtaaat catcgtcgta gagacgtcgg aatggccgag cagatcctgc
     1801 acggttcgaa tgtcgtaacc gctgcggagc aaggccgtcg cgaacgagtg gcggagggtg
     1861 tgcggtgtgg cgggcttcgt gatgcctgct tgttctacgg cacgtttgaa ggcgcgctga
     1921 aaggtctggt catacatgtg atggcgacgc acgacaccgc tccgtggatc ggtcgaatgc
     1981 gtgtgctgcg caaaaaccca gaaccacggc caggaatgcc cggcgcgcgg atacttccgc
     2041 tca
//
LOCUS       DAJTYH010000242         1983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTYH010000242 DAJTYH010000000
VERSION     DAJTYH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(121..528)
                     /locus_tag="OO609_004106"
     CDS             complement(121..528)
                     /locus_tag="OO609_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6635288.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            605..832
                     /locus_tag="OO609_004107"
     CDS             605..832
                     /locus_tag="OO609_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6635289.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            816..1238
                     /locus_tag="OO609_004108"
     CDS             816..1238
                     /locus_tag="OO609_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635290.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1316..1954
                     /locus_tag="OO609_004109"
     CDS             1316..1954
                     /locus_tag="OO609_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635291.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMSGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPVEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGDTGTGKIPSAGFYT"
BASE COUNT          540 a          406 c          525 g          512 t
ORIGIN      
        1 gaataaccta aggagggtga tcaagatttt tgtgtagaaa aacctaagtt ttttgatgta
       61 aaaaacacaa gtgtttgaaa gtttgtgctt tttattacag ggtgtggaga aaaaagggga
      121 ttatttgttt gcgcttcttt tgcgagcttt gagtagttct tcaaaaagtt tgttgaaatt
      181 ttcaactcga gcacgcatct ctgacaacag ggccttttgc tctgactcag gcagtgcgtc
      241 gaacagttga agtaactctt tttgatcttc tgtcagaatg gctggctgat tatccgggat
      301 cggttcgcct ggttgtttat cttcatcccc aaaaagaagc caagtcggtg agcactgaag
      361 cgcttggctc agtgcgaata atcttttccc cgccggctgt gtttcatctc tttcccattg
      421 agaaattgtt acgtgagcca ctttgaccag cttacctaat gcggcctgag acagttttaa
      481 ttttttacgc ctatgtaaga ggcgagcacc gaaggtttcg tttttcatat tagggaattc
      541 taatttttct tgacttaggt ttctctacgg cctggtttcc ttaggaaaat ctaaggagct
      601 cgatatgttg aaaattgatg ctatagcgtt ttttggcagc aaaacaaagc ttgccaatgc
      661 cgcaggagtt aggctggcaa gcattgctgc atggggggaa ctggttcctg aaggtcgcgc
      721 gatgcgcctg caagaggcat ctggcgggga acttcagtac gaccccaaag tttatgacga
      781 atatcgtaag gcaaaacgac ctgggaaggt gattcatgaa aatcaggcat gagcacatcg
      841 aatcagtgct gttagccctg gcagccgaaa aagggcaggc gtgggtcgct aacgcaatta
      901 ctgaagaata tctgcggcag gggggcggcg aattgtctct ggtaccaggc aaggactgga
      961 acaatcagca gaatatctat caccgttggt tgaaaggtga aacgaaagcg caaagggaaa
     1021 aaattcagaa actgatccct gcggttctgg caattcttcc gcgtgagctg cgtcaccgac
     1081 tctgtatctt cgataccctg gaacgccgtg cattactggc ggcgcaggaa gcgttgagta
     1141 cggcaattga tgcgcatgat gatgctgtcc aggccgttta ccggaaagca catttcagcg
     1201 gtggtgggtc gcccggcgat tctgtcgtag tgcattgatt gaaattaatc gtgccggatt
     1261 gttttgttcg gtatcagtta aatgtaacgc tgcgagcgtt acaaggtgaa aacaaatggc
     1321 ttcaaactgg ataaagctcg aggttattac gccggataag ccggaaatat tcaggcttgc
     1381 tgagattctg aatattgatc cagatgccgc attagggaag gttattcgct tctgggcatg
     1441 ggcggatcaa caaatgatag acggtaatgc agattgtaac gctcgcggcg ttacaaaaag
     1501 tgcaatagat cgcatcactt ttatgtctgg ttttgctgat gcgttaattc aggttggatg
     1561 gctggtcgaa aatgacggtg ggctttctct acctaacttt gaacgtcata acggaaaaag
     1621 ctctaaaaaa cgggcggtta caaacgagcg agtaacaaaa atacgcgaac tgaaacgaaa
     1681 aggtaacgct gccagcgtta cacaaacgga tcaaaaagcg ttaccagtgg aagaggaaga
     1741 ggaagatcta aatactgatc tccccctaaa tccccctcgc caaaaacgag cgtctaaaaa
     1801 attcgagccg gaggctattg agctgcccga ttggttgccg gaaacactct ggcatgagtg
     1861 ggtccggttc agacaggcat tgcgaaaacc gattcgaacg gagcagggcg atacgggaac
     1921 tggaaaaatt ccgtcagcag ggttttacac ctgagcaggt gattcgacac agcatcgcca
     1981 atg
//
LOCUS       DAJTYH010000243         1979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTYH010000243 DAJTYH010000000
VERSION     DAJTYH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(38..643)
                     /gene="azoR"
                     /locus_tag="OO609_004110"
     CDS             complement(38..643)
                     /gene="azoR"
                     /locus_tag="OO609_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6635292.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            844..>1979
                     /locus_tag="OO609_004111"
     CDS             844..>1979
                     /locus_tag="OO609_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6635293.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYGYRT"
BASE COUNT          502 a          514 c          514 g          449 t
ORIGIN      
        1 actgaatatc tacagtccac atcaagaccg tgtccggtta tgcagcaaca atgctgtcga
       61 tggctgcttt tgcgtcagac tgtgctttcg ctgccatttc cggaccgtat gcgatccctt
      121 cggcgaagac aaatttcaca tcggtaatgc cgataaagcc gaggaacgtg gacagatacg
      181 gcgtcaccag gtccgttggt ccatctttgt ggatcccacc gcggctggta ataacgatgg
      241 cttttttacc cgttaccaga ccttccggac cgttctcggt atagcggaaa gtaacgcctg
      301 cgcgtgcaac caggtcaaaa tagtttttca actgagttga gatgttgaag ttatacatcg
      361 gtgccgcaat aacgataacg tcgtgggctt tcagctcggc aatcagctca tcggaaagcg
      421 ccagagcttc ctgctgacgc ggagtcagcg gcgcatcgct cggacgcaga gcgccaacca
      481 gttcgccatc cagtaccgga atcggatttg cagctaggtc gcgaacggtg atttcatcag
      541 cggagtgctt ttcgctccat tgttcaacaa aataatcgga caactgatta gactgagagt
      601 accctgccag gatgctggat ttaagaacta ataccttgct catggtgttt ccttatagat
      661 gtttgaatgg acgatgcccc gttgcttgct gacactttat tcacaatcct gccacagaga
      721 tcgcgcaata aatcgaagtc tatgttcgaa tttattgaac aacgcataga aagccgcgat
      781 gtggtactct atatctatca tttaaaagaa aattaatcag gcagactact gcccactaac
      841 gttatgacag aacaacaaaa attgaccttt actgccttgc aacagcggct ggattcgctg
      901 atgctgcgcg acagactgcg tttttctcgc cgtctgcatt gtgttaagaa ggttaaaaat
      961 cctgatgcac aacaggccat tttccaggag atggcgaaag agattgacca ggcggcaggg
     1021 aaagtcctgc tgcgtgaagc ggcacgaccg gaaatcactt atcctgacaa tttaccggtt
     1081 agtcagaaaa aacaggacat actcgaagcg attcgtgatc accaggtggt gatcgtcgcc
     1141 ggggaaaccg gctccggtaa aacgactcag ctaccgaaaa tctgtatgga gctggggcgc
     1201 ggtattaaag ggctgatcgg tcatacccag ccgcgtcgcc tggcggcacg aacggtggcc
     1261 aaccgtattg cggaagagct gaaaacggag ccgggcggtt gcatcggtta caaagtgcgt
     1321 ttcagcgatc acgtaagtga taacacgatg gtcaagctga tgaccgacgg gatcctgctg
     1381 gcggagatcc agcaagaccg cctgctgatg cagtacgaca ccatcattat tgacgaagcg
     1441 cacgaacgca gcctgaatat cgattttctg ctcggctatt tgaaagagtt gctgccgcgg
     1501 cgtcctgacc taaaaatcat tatcacttcc gcgactatcg acccggaacg cttttcgcgc
     1561 cactttaata atgcgccgat tattgaagtc tccggtcgga cctatccggt ggaagtgcgc
     1621 tatcgcccga ttgttgaaga agccgatgac accgagcgcg atcagttgca ggcgattttt
     1681 gacgccgtag acgaactaag ccaggaaagc cccggcgaca ttctgatctt tatgagcggc
     1741 gagcgggaaa ttcgcgatac cgccgatgcg ctgaacaagc tgaacttacg ccataccgaa
     1801 atcttgccgc tttatgcgcg gctttcgaac agcgaacaga accgcgtgtt ccagtcgcac
     1861 agcggacggc gcattgtgct ggcgaccaac gtcgcggaaa cctcgctgac cgtaccgggg
     1921 attaaatacg tcatcgaccc cggtacagcg cgtatcagcc gctacggcta tcgcaccaa
//
LOCUS       DAJTYH010000244         1957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTYH010000244 DAJTYH010000000
VERSION     DAJTYH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..597)
                     /locus_tag="OO609_004112"
     CDS             complement(<1..597)
                     /locus_tag="OO609_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6635294.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAA"
     gene            complement(548..1312)
                     /locus_tag="OO609_004113"
     CDS             complement(548..1312)
                     /locus_tag="OO609_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635295.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1507..>1957
                     /locus_tag="OO609_004114"
     CDS             1507..>1957
                     /locus_tag="OO609_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6635296.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLIS"
BASE COUNT          477 a          487 c          433 g          560 t
ORIGIN      
        1 cgccgcgtcg ataaccttaa gcatggtgtc agacagaaac ggattttcgt catagttaat
       61 tttgcggatg agagtgcctt cgggcggatc aacaacgaag ttgcgccaga cgaaatcagt
      121 aacaagtccg gggttgaata tgaaccagca ttccgaaccc tctttacgga tcgttggctc
      181 cagaattttc cactggtatt ccgtcagtgc gtgggcttct tccaaccaca acatatcgat
      241 cccctccagt gacttaattt cttcgatgtt gcgccataat ccataaaaaa caaattccga
      301 gccagtaacc cggttaatga ttttgttgtt cagaatacgg aaacggtgcc gcagaccaaa
      361 cctgtctatc tgaattttga gcagggtata caccgactct tcgattttat tctggatctg
      421 acgggcacaa caaaaacgca gggtgtattt attcgacaga aatatggcaa tgccagcggc
      481 atcccatgat tttgacgatg accgaccacc ataaagcact ttgttacgtg cccgcatagt
      541 ccagaaacta cgtaaagccg gattcagcgt cggtttggat gtcagagtag aagtcattga
      601 ggtcacgctc tccattgcca tcatcaatac ctgcatcacg gcgaagacga tcagcctcca
      661 gagacacctt atcagtggcg gccttgcggt aggctgtatc agcaaatatt tttcctaccg
      721 tcgcaagcgt gccaacgatg gactcaatac gaactgtatt gcgcatcatt gctttctcgg
      781 cggcgctgat attttcaatc agcatcttcc tttcctggtc cccattagca tcttccagcg
      841 acaccaacca ccgaccaata ttctctgcag cgacaaggtt gttagcacga aggcgaaata
      901 attcgtcctc gagcgtcaac gctttagcgt cctctatcac ctcatctttg agcagaaggc
      961 gacgggcata accaccgtgt tttaatgcct gctggttacc gggttggaat gggttagtcg
     1021 ggggatcggt acgcattccg cgtatcggtt tcgtatccgg tgtagattct gtttttagtt
     1081 gcgtactttt ttgtgtaagg ccagtaatgg caggctttct gctggtacgc actttccttt
     1141 tttgcgtacc atttttgcaa acctgcgtac cgccactgcg tgcccaatca agctttttgg
     1201 ccctcttcct gatagcccct tctgtaacgc cgtatttctc gcctatatca cggaggctaa
     1261 ggactccggc ccggtatgcc gattcgatgg cctcccagtc cggttttgcc atgattatgc
     1321 ttcctgtgat ttactgttat cgctgcctgt cacaataggt tgctttaatt cttcctcatc
     1381 actcactcct gcgacgctcc gtggcagggc cccccacagg agtaccttgt aaatgtaagg
     1441 tgtttaagcc taaattaagt cgtatcacgg ttaaatagca aagaaaaatt cacagaagga
     1501 aagacattga tggagtggat aaatgctttg atgaactatt ttgtacttca tccatatcgc
     1561 ctatgggggc ttctttttat aatagcgttc acaaaatcca ctttactcat ttcggcgata
     1621 ttgccaccag catcaattat gatggccact gtaattacag tcagcaagac gactctttca
     1681 ccatgggaag ctggaatcac ggtcatgagt ggcgcatggt gcggtactat cgttaattac
     1741 catcttggta taataattgg gcatatccct caacttgcat gtataatatc gagccgttcc
     1801 aatacaatag aacgggttcg ccttcagttg caaaataact ctgtgtcaat acttttcact
     1861 tcgcgcttta tcgccgtgct gcgttacatt gctcctttgg tggcaggaat gttgcaactt
     1921 catccagtga aagtgtatac agtgagtctg atttcag
//
LOCUS       DAJTYH010000245         1899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTYH010000245 DAJTYH010000000
VERSION     DAJTYH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            6..224
                     /locus_tag="OO609_004115"
     CDS             6..224
                     /locus_tag="OO609_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6635297.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO609_004116"
     CDS             226..591
                     /locus_tag="OO609_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6635298.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO609_004117"
     CDS             588..1253
                     /locus_tag="OO609_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635299.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO609_004118"
     CDS             1253..1618
                     /locus_tag="OO609_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6635300.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          521 a          384 c          507 g          487 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattg
//
LOCUS       DAJTYH010000246         1891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTYH010000246 DAJTYH010000000
VERSION     DAJTYH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..149
                     /locus_tag="OO609_004119"
     CDS             <1..149
                     /locus_tag="OO609_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR6635301.1"
                     /translation="DQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYL
                     SEKQ"
     gene            173..463
                     /gene="lsrG"
                     /locus_tag="OO609_004120"
     CDS             173..463
                     /gene="lsrG"
                     /locus_tag="OO609_004120"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6635302.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            503..1261
                     /gene="tam"
                     /locus_tag="OO609_004121"
     CDS             503..1261
                     /gene="tam"
                     /locus_tag="OO609_004121"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6635303.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1265..>1891)
                     /locus_tag="OO609_004122"
     CDS             complement(1265..>1891)
                     /locus_tag="OO609_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635304.1"
                     /translation="LREVKTTLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHY
                     LKTGDLQRVLASNSPANRILLIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGC
                     ERIAYTPIPFAYTLILHRTVYLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAE
                     EPEDPFGTENNDLPLDAICNAIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          487 a          454 c          495 g          455 t
ORIGIN      
        1 tcgatcaggg cgcttctggt gtggatatgg ggcgtaatat tttccagtct gaccatccgg
       61 tggcgatgat gaaagccgta caggcggtgg ttcaccataa cgaaacggct gatcgggcat
      121 atgaactcta tctgagtgaa aaacagtaac tgcggattta aggagaagaa ttatgcacgt
      181 cacactggtt gaaattaacg ttcatgaaga caaggttgac gagtttatcg aagtttttcg
      241 ccagaaccac ctgggctctg tacaggaaga aggtaatttg cgcttcgatg tcttacagga
      301 cccggaagtg aattcgcgct tttatattta cgaagcctat aaagatgaag acacagtggc
      361 attccataaa accacgcctc actacaaaac ctgtgtcgcg aaactggaat ctttaatgac
      421 cgggccgcgt aaaaaacgtc tgttcaatgg gttgatgccg tgaggctaca attggggtaa
      481 cgcgctgacg ggagtaaaaa aaatgtctga ctggaacccc tctttatatc tgcacttttc
      541 cgctgaacga tcgcgtccgg cggtggagct gcttgccaga gtgccgctgg agaatgtcga
      601 atatgttgcc gatcttggct gtggcccagg taacagcacc gccctgctgc atcaacgttg
      661 gcctgcggcc aggataacag gcatcgactc gtctccggcg atgattgctg aagcgcgcag
      721 tgctttgcca gactgccagt tcgtggaagc agacatccgc aactggcaac cggaacaggc
      781 actcgatctg atttttgcta atgcctcact gcaatggctg cccgaccact acgaattgtt
      841 tcctcacttg gtttctttac ttaatccgca gggcgtgctg gcagtacaga tgccagataa
      901 ctggctggag ccgacccatg tgctcatgcg cgaagttgct tgggaacaaa actacccgga
      961 tcgcgggcgt gagccgttgg ctggcgttca tgcttactac gatattttga gcgaagccgg
     1021 atgtgaggtc gatatctggc gaaccactta ctatcaccag atgccgtcgc accaggcgat
     1081 tatcgattgg gtgaccgcca ctggattacg tccgtggtta caggatctga ccgagagcga
     1141 acagcagctt tttcttacgc gctaccatca gatgctggaa gagcagtatc cactgcaaga
     1201 gaacggacag atactgctgg catttccgcg tctgtttatt gttgcccgcc gtacggagta
     1261 attatcacgt cagctggtaa tgacgatcgg gaagaatttt tgctggaatt tctgcttcat
     1321 cgttcatctg taacaggtca atttcaatag cgttgcagat ggcatccagc ggtaaatcat
     1381 tgttttcagt accgaacgga tcttccggtt cttccgccag acaatccagc gaaataaaag
     1441 tgtaggaaat cagcacagag ataaaaggcg tcatgtaatg caggtccacg accagcgcga
     1501 acggcagcat gatacaaaac agataaacgg tacgatgcaa aatcagggtg taggcaaagg
     1561 gaattggcgt gtaggcaatg cgctcacagc ctgccagaac cgctgaaata tcattaagcc
     1621 gatcgttgag gctaataaac aggatatctg aaagctgtcc attgcggcgc tgaaccgcca
     1681 accattctcc cattattaac aagatacggt tagctggaga gttcgaagcc agtacacgct
     1741 gaagatctcc agtcttgaga taatgagcca gcacttccgc ctgtggctgt ttgcgtaatg
     1801 tcatgcgtaa acagtgggcg aaggcgattt gcagcctggc aaactccctt acacttgccg
     1861 aatccggcaa tgtcgttttt acctctcgca g
//
LOCUS       DAJTYH010000247         1880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTYH010000247 DAJTYH010000000
VERSION     DAJTYH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            32..211
                     /locus_tag="OO609_004123"
     CDS             32..211
                     /locus_tag="OO609_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635305.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            263..457
                     /locus_tag="OO609_004124"
     CDS             263..457
                     /locus_tag="OO609_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635306.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1256..1592)
                     /locus_tag="OO609_004125"
                     /pseudo
     CDS             complement(1256..1592)
                     /locus_tag="OO609_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          587 a          398 c          388 g          507 t
ORIGIN      
        1 actacagcgc attgctttac gagtaataac aatggattta tcaccagaag acgcaaagtt
       61 tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc aaacgggcat
      121 gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg acgacaattc
      181 caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac agtggtgtga
      241 tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg agcaactgtt
      301 acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc cacatgaacg
      361 cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa tcaggtctga
      421 taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat attgccacca
      481 accacagatg tggcatcgtt atcagcgcac ttgattttct ttatattcac tgacgagctt
      541 gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac ggcgtacaag
      601 cccactagca tgacattgta ataaggcctg agcctaacgc tttaatgtgt gtcatacact
      661 cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc gaaatccatc
      721 tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat ctgcgcccaa
      781 tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg ttacagagtt
      841 ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta accgggaaat
      901 tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg atgctatacg
      961 atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc agggttggca
     1021 atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca aaatatgccg
     1081 cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac actgctaaaa
     1141 cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca gaatctgcat
     1201 tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac ttacactata
     1261 cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta gttgtcacaa
     1321 tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt aattctgcgt
     1381 tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca gttccccttt
     1441 ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc ctggattata
     1501 gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc agatccagtc
     1561 cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat tgagtcgaaa
     1621 ataagcacta ctctatacag gataattaga ggtcagtttg attattcaca attcatcatc
     1681 agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata attattataa
     1741 taacggcttt aattggaata catatattac aacgtattat atataattgg tattctggga
     1801 actatattct caaaatacag tagaaacgag gtatgtttct ggtggaaagg acagtgggat
     1861 taaaaagtaa gaattgataa
//
LOCUS       DAJTYH010000248         1873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTYH010000248 DAJTYH010000000
VERSION     DAJTYH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>1873
                     /locus_tag="OO609_004126"
     CDS             <1..>1873
                     /locus_tag="OO609_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6635307.1"
                     /translation="IINDKENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQ
                     RLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQ
                     HGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLS
                     KRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKL
                     AAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQII
                     AVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNT
                     LLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLM
                     YETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALIT
                     RYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRM
                     ALDAFNVALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLT
                     LANEVMVKRLTKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWT
                     "
BASE COUNT          548 a          436 c          446 g          443 t
ORIGIN      
        1 atatcattaa tgataaagaa aaccgggtga acggcatcgc actctattat cgcctgccaa
       61 tggtacaagt gaacgatgag caaagctttg tcgaacaagc tgaatggagc atgttagttc
      121 agctgttcaa tcaacgtctg caggaacgca tacagtcggg cgagttgaag actatttctg
      181 gcggcactgc gcgcagcgtt aaaattgcac ccgattatca gtcgctgttt ttccgtgtaa
      241 atgcacgaga cgataatatg caggatgctg cgaatgcatt aatggcagag ttggcaacca
      301 ttgatcagca tggtttttct gctgaagaac tcgatgatgt caaatctacc cgcctcacct
      361 ggctgaaaaa tgcggttgat cagcaagctg aacgtgattt acgtatgctg accagtcgcc
      421 tggcatccag ctcattaaat aatacgccgt tcttgtcgcc ggaagagaca tatcaacttt
      481 cgaaacgtct gtggcagcaa attaccgtgc aaagtctggc ggaaaaatgg cagcagttaa
      541 gaaagaacca ggacgcattt tgggagcaaa tggtaaacaa tgagcttgcc gccaaaaaag
      601 cattgtctcc tgcggctatc ctggcgctgg aaaaagagta cgccaacaaa aagctggcgg
      661 cttacatctt cccaggcaga aatttatcgt taacagtaga cgctgaccca caggcggaaa
      721 ttagcagcaa agaaacgctg gctgagaatc tgacatcatt aacactttcc aatggtgcca
      781 aggttattct ggcaaaatcc gcgggtgaag agcaaaagct acaaattatt gccgtatcga
      841 ataaaggcga tttaagtttc cctgcgcagc aaaaatcact tatcgcgctg gcaaataaag
      901 cagttagcgg aagcggcgtt ggcgaactct cctcttccag cctgaaacgc tggagtgcgg
      961 aaaactcggt aaccatgagc agtaaagtca gtggcatgaa tacgttgctc tccgttagcg
     1021 cgcggactaa taaccctgaa cctggtttcc agttgattaa ccagcgaatc acccacagca
     1081 cgattaacga caatatttgg gcatcgctac aaaatgctca aattcaggcg ttgaaaacgc
     1141 tcgaccagcg tccagcggag aaattcgccc agctgatgta tgagacgcgc tatgctgatg
     1201 accgcacgaa attactgcaa gaaaatcaga ttgcacagtt tactgccgca gatgcgctgg
     1261 ctgccgatcg ccagttgttt tcatctccag cggatatcac gtttgtcatt gtcggtaatg
     1321 tcgcagaaga caaactcgtg gcgttaatta cgcgttactt aggctcaatc aaacactctg
     1381 attcgccatt agccgcaggt aaaccattaa ctcgcgcgac ggacaacgca tcggttactg
     1441 taaaagaaca aaatgaacct gtggcacagg tttcacagtg gaagcgttat gattcccgga
     1501 cacctgttaa tctggcgacg cgtatggcgc tcgatgcttt taacgtcgca ctggcaaaag
     1561 atctacgcgt taatattcgt gaacaggcat ctggggcata cagcgtttct tctcgcctct
     1621 cggttgatcc tcaggctaaa gatatcagtc atttgctggc ttttacttgt caaccagaac
     1681 gacatgatga acagttaacg ttagcgaatg aagtgatggt taagcgtttg actaagggga
     1741 tcagtgagca agaactgaac gaataccagc aaaacgttca gcgcagcctc gatatccaac
     1801 agcgtagcgt tcaacaatta gcgaacacta ttataaatag tcttattcaa tatgacgatc
     1861 ctgcagcatg gac
//
LOCUS       DAJTYH010000249         1831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTYH010000249 DAJTYH010000000
VERSION     DAJTYH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            200..343
                     /locus_tag="OO609_004127"
                     /pseudo
     CDS             200..343
                     /locus_tag="OO609_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            490..756
                     /gene="ospE1"
                     /locus_tag="OO609_004128"
     CDS             490..756
                     /gene="ospE1"
                     /locus_tag="OO609_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR6635308.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1146..>1831
                     /locus_tag="OO609_004129"
     CDS             1146..>1831
                     /locus_tag="OO609_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6635309.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPE"
BASE COUNT          626 a          382 c          309 g          514 t
ORIGIN      
        1 aacgccaacc ggtgtatatt tcatttgtgg agtctccagt ctgaggacgc tttatgcatc
       61 ccgtattggg cagtatgatg ccggaaagct gcgaggctcc acacctgcca gtttcaccct
      121 tttaaccatc atactactcc gtgtttaatt gtgtgggatg cgttcattac attttaacag
      181 tgtcaccccg tcggccacga aagataaaca ggtgaccaga aaagggatct tcatccagca
      241 catgctatat cttgaacgta acaacctgac cctccgcacc cgcatcaagc ggctggcacg
      301 taaaacaatc tgcttctcgc gttccgtgga aatccacgaa aagtcatcgg ctccttcatt
      361 gaaaaacaca tattctactg attggaagcg ccacccgaaa aagtatcgtt tttttacagt
      421 aaacttcatt tagccgacta taatgtaaaa aaataaattt tttaccaaaa tataacataa
      481 ggaagtaata tgcttacaca aactatattc ccttgtcttc cacaaaaaca agaaaatatc
      541 atccttgaag tatcaaatcc tgtattatta tcttctactg ttactacaga tggatatact
      601 gttttcaata aaaaagcagc tatatacgaa ttacaaatac ctgctgccag ccggacgaaa
      661 acgttgaaat tcactgctac agaaatgcaa tggttgacaa agataaatga agccggaatt
      721 gatgaaaaac aaagccaacg gtattctgat ttctgaagtt aatttcagac tcgtttaagc
      781 tccattttca aaaaaaatat atcagggttt gttactaata acttctgaaa acagaaaaaa
      841 atagagcagt aattctattt cagagaggag taactccatc tatcctcgat acgtactgaa
      901 cgttatcgtg atatattcat catgctttct tcaccattat tcgagtatag ggagagacgc
      961 aatgacctta aatacctttt tatggctgaa ttaccattct caggaataac agcagactcc
     1021 ttcccataga cggcttaggg ggaaagcaat acccggagaa agggtacaaa gcaaactgtt
     1081 ttaaaattaa taagccatat tctatttaac tagaattctt atcgttgata agaattctag
     1141 ttataatgat taaatcaacc aatatacagg caatcggttc tggcattatg catcaaataa
     1201 acaatgtata ctcgttaact ccattatctt tacctatgga actgactcca tcttgtaatg
     1261 aattttattt aaaaacctgg agcgaatggg aaaagaacgg taccccaggc gagcaacgca
     1321 atatcgcctt caataggctg aaaatatgtt tacaaaatca agaggcagaa ttaaatttat
     1381 ctgagttaga tttaaaaaca ttaccagatt taccgcctca gataacaaca ctggaaataa
     1441 gaaaaaacct attaacacat ctccctgatt taccaccaat gcttaaggta atacatgctc
     1501 aatttaatca actggaaagc ttacctgcct tacccgagac gttagaagag cttaatgcgg
     1561 gtgataacaa gataaaagaa ttaccatttc ttcctgaaaa tctaactcat ttacgggttc
     1621 ataataaccg attgcatatt ctgccactat tgccaccgga actaaaatta ctggtagttt
     1681 ctggaaacag attagacagc attcccccct ttccagataa gcttgaaggg ctggctctgg
     1741 ctaataattt tatagaacaa ctaccggaat taccttttag tatgaacagg gctgtgctaa
     1801 tgaataataa tctgacaaca cttccggaaa g
//
LOCUS       DAJTYH010000250         1816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTYH010000250 DAJTYH010000000
VERSION     DAJTYH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(198..599)
                     /locus_tag="OO609_004130"
                     /pseudo
     CDS             complement(198..599)
                     /locus_tag="OO609_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1028..1816
                     /gene="virF"
                     /locus_tag="OO609_004131"
     CDS             1028..1816
                     /gene="virF"
                     /locus_tag="OO609_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6635310.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          566 a          329 c          336 g          585 t
ORIGIN      
        1 tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac
       61 attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga
      121 gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt
      181 cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag
      241 agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg
      301 ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc
      361 atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc
      421 agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg
      481 gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat gacatcctgc
      541 accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa
      601 tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc
      661 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
      721 ataggatatt atgatgctgg agttttgcaa agctccactc ctttccgaac atttacacag
      781 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      841 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      901 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      961 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
     1021 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
     1081 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
     1141 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
     1201 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
     1261 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
     1321 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
     1381 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
     1441 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
     1501 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
     1561 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
     1621 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1681 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1741 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1801 tcataaaaaa ttttaa
//
LOCUS       DAJTYH010000251         1802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTYH010000251 DAJTYH010000000
VERSION     DAJTYH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..269)
                     /locus_tag="OO609_004132"
     CDS             complement(<1..269)
                     /locus_tag="OO609_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="member of the iron-containing alcohol dehydrogenase
                     family; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6635311.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVV"
     gene            378..1538
                     /gene="ybdL"
                     /locus_tag="OO609_004133"
     CDS             378..1538
                     /gene="ybdL"
                     /locus_tag="OO609_004133"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6635312.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            complement(1539..>1802)
                     /locus_tag="OO609_004134"
     CDS             complement(1539..>1802)
                     /locus_tag="OO609_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6635313.1"
                     /translation="GYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIVRELSQL
                     GWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
BASE COUNT          412 a          470 c          473 g          447 t
ORIGIN      
        1 accacgctgc gatcgtcacc ggactcagcc gccagttgct gtacatcgct ttcgctgcaa
       61 tgaccacgaa acaaaatatg ctttgcccct ggaagtccaa acgctggcgg aagtttggtt
      121 tgcgccgcag caatggcgcg tttgccgtag atccacaccg cgcgagaaag ttgttcatca
      181 gtgaaaaaat tgtgcaggtg attgaaactt cctggatgtg aaaagtagtt agccgggccg
      241 acgaccacgc ggataggatt gtgaggcatg ttgtgttgtc cttattgttt tatttagaca
      301 tctaaacgtc ttgattgcca aatactggca tcgtgttata gtgccttcaa cacgcaactt
      361 cgtcaggtac aataaaaatg acaaataacc ctctgattcc acaaagcaaa cttccacaac
      421 ttggcaccac tattttcacc cagatgagcg cgctggcgca gcaacaccag gcgattaacc
      481 tgtcgcaagg ctttcctgat tttgatggtc cgcgctattt acaggagcgg ctggcgtacc
      541 acgttgccca gggggcaaac caatacgcgc ccatgaccgg cgtgcaggcc ttgcgcgagg
      601 cgattgctca gaaaacggaa cgtttgtatg gctatcaacc agatgccgat agcgatatca
      661 ccgtaacggc aggggcgacg gaagcgttat acgcggcgat taccgcactg gtgcgcaatg
      721 gcgatgaagt gatttgtttt gatcccagct atgacagtta cgcccccgcc atcgcgcttt
      781 ctgggggaat agtgaaacgt atggcactgc aaccaccgca ttttcgcgtt gactggcagg
      841 aatttgccgc attattaagc gagcgcacca gactggtgat cctcaacact ccgcataacc
      901 ccagtgcaac tgtctggcag caggctgatt tcgccgcttt gtggcaggcg atcgccgggc
      961 acgagatttt tgtcattagc gatgaagtct acgagcacat caacttttca caacagggcc
     1021 atgccagtgt gctggcgcat ccgcagctgc gtgagcgggc agtggcggtt tcttcatttg
     1081 gcaagaccta tcatatgacc ggctggaaag tgggttattg tgttgcgcca gcgcccatca
     1141 gcgccgaaat tcgcaaggta catcagtatc tgaccttttc ggtgaatacc ccggcacagc
     1201 tggcgcttgc tgatatgcta cgtgcagaac ctgagcatta tcttgcgtta ccggactttt
     1261 atcgccagaa gcgcgatatt ctggtgaatg ctttaaatga aagccggctg gagattttac
     1321 cgtgtgaagg tacatacttt ttgctggtgg attacagcgc ggtttctacc ctggatgatg
     1381 ttgagttttg ccagtggctg acgcaggagc acggcgtagc ggcgattccg ctgtcggtgt
     1441 tttgcgccga tcccttccca cataaactga ttcgtctctg ttttgccaag aaggaatcga
     1501 cgttgctggc agcagctgaa cgcctgcgcc agctttagct atttaaccgt ccaggcgcgg
     1561 gaatattgac ggtcggcaaa caactcttgc aggccgttaa tttgcttcag tcgcagcact
     1621 tcgtcgctgt ccattcccag ctctttgcca attttattat catcccatcc caactggcta
     1681 agttcacgga cgatttccga catggcggtg atttgatggc gtccacgagc gcgattatga
     1741 cgaatagtcg cggcgatgcg ttgattacgc gtaccttcca gacaggtcac cggcaaataa
     1801 cc
//
LOCUS       DAJTYH010000252         1783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTYH010000252 DAJTYH010000000
VERSION     DAJTYH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            128..432
                     /locus_tag="OO609_004135"
                     /pseudo
     CDS             128..432
                     /locus_tag="OO609_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            601..>1783
                     /locus_tag="OO609_004136"
     CDS             601..>1783
                     /locus_tag="OO609_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6635314.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          461 a          347 c          413 g          562 t
ORIGIN      
        1 aagccatacc cgctatcggc gatgaaaatg cttttgtgcg cggtcggagt ggtcccgacg
       61 agggttttac ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg
      121 agcgtgtatg agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga
      181 aataaaacca gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact
      241 acaagatttt atttactggc gtcctgatgc ggacagggct ggtatggaac ctgtttatgt
      301 gatacttaat gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat
      361 cttaaacatg caagtaattt tggtattctc gatacaagaa taaatagtag taactactat
      421 agctgcaaat aatgttaaaa acatttctat gatcatcttt acatttttca tgattgacaa
      481 ttgtttttgg ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta
      541 tataatgata agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat
      601 atgtcattat tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga
      661 ttatatcccg tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat
      721 tattttgtcc gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag
      781 atcggcatta tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga
      841 cttatttctg acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga
      901 ctggtaaatt ttctgatagg atatattccc gggatcatca catccgttac gttattttca
      961 acaatgtatt tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca
     1021 ctggtgttct ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg
     1081 cacaatgtcg ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc
     1141 gacagtgctc tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca
     1201 atgagtgtgc tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc
     1261 attgaagaat ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg
     1321 agactctcca ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac
     1381 tgctgtatct atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct
     1441 aagtttttaa gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg
     1501 ggggggggtt ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg
     1561 tctgtcagac tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc
     1621 attattttat taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac
     1681 tatacaatcc ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt
     1741 tattcaatgg agcttgttcc taaagaagct gccggagccg cat
//
LOCUS       DAJTYH010000253         1753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTYH010000253 DAJTYH010000000
VERSION     DAJTYH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(15..548)
                     /locus_tag="OO609_004137"
     CDS             complement(15..548)
                     /locus_tag="OO609_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6635315.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(614..1243)
                     /locus_tag="OO609_004138"
     CDS             complement(614..1243)
                     /locus_tag="OO609_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635316.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1247..1462)
                     /locus_tag="OO609_004139"
     CDS             complement(1247..1462)
                     /locus_tag="OO609_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6635317.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          463 a          470 c          367 g          453 t
ORIGIN      
        1 cgcggtgact ctgctcattg atctatcccc caacaggcta atgcgctttc ctggtcacga
       61 cgaataacct gtccatagca gttatttgaa cgtatgcggc aatcgcgccc aacatctttt
      121 atccaccagc gaatcgcctc gcatgcgccc ttacgatcac cggcattcag ccgcttataa
      181 aacgtcgacg ggaaacactt accggggcca atgttatagg ggcaaaatga cgctataccc
      241 gctttctggg gttcggtcag aggtacttta atattgcgct ccacccatgc cagcgcctta
      301 tcacgttcaa tagcgttaac ctggtcacat ttttccttcg acagcttcat tcccggtatg
      361 acaggcttac catccaccat tgtggcacca cgacagatgg tccatatgcc agaaccatca
      421 cggtatgccg ttgtgtgatt accctctttt tcgttcagaa actggtcaag tatctgggga
      481 gcagacgcgc ctgcggcaat cagcgccaga acagcagccg acaggccgta tttgattttt
      541 gcgctcatgg atatttatca ggatttatcg gcaacaaata acgagccaac ttatatacgc
      601 cttttaagat aagtcagccc cggctggatc cagtcatcag gctctttctt aaagggggag
      661 tattgaaatc acgaagaaga gcctcccgca ccgcagcatc catgtctgca ccactggcaa
      721 gtgcttcaat ctctgccgcc acctgcaaat accccatgca acggccaatg cgtttcataa
      781 ctctggtgtt ttcgtctttc aggcatctga tagcgagatt aataattttc aggcagtgat
      841 gatgcgatgc ccggacaggt tttccgttct cataaacgac gaactcaccc tcttcacagg
      901 aaaccctgaa aggccgatgg ggttcctggt ctttatccgg tgcagacgca ataaaatcac
      961 ttttatgctc ttcctctccg gcactgttaa ccgtaatgac gtactcaggc cgcaggtaag
     1021 ccgcctcttt ttctgacaat gcggccggta aaaacttttc ctgcaaaaac ttcataaaag
     1081 tctcgtgagc gactttgtgg cagtaatcgt aaatcgccgc ataatgctca tcgcggcgtt
     1141 tgttttcatc ttcagaactc accaacgccg acaatttttt attaagctca gtgatttcat
     1201 tttccaggtg attgaatcgc tgattcattt cttcatgctt cattatttac tctccccgtg
     1261 cagccttacg cttgtcttct cttattttga aatacagatt cgtcagataa gtcagaagcc
     1321 ccagaagcag acttcccagc acaccaatcg cagcccactg tgatggcctg acctgatcca
     1381 accactgcaa aaaccagtag ccggcactgc cggcggaggt gccgtaggca atgcccgttg
     1441 aaattttgtc catggatttc atagcctcac ctccgcaaat aacggatggc gtagttttac
     1501 actgagaaat gaaagggatt tgaaaagaaa aaaacgcaaa agcgggcgaa acgatatata
     1561 cagtaaggaa agcactctat ccaacaaacc acccacagtt aatcggaata aaagcagagt
     1621 gcttatgaat gatcgcacgc ccgaaggtta gtatttctgc acagcaattt tgcaaaaaaa
     1681 ggcgatcatt cataacttaa acgtctttca gtcactccgg gatttcccat catcgcagac
     1741 tgaaagactc tga
//
LOCUS       DAJTYH010000254         1751 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTYH010000254 DAJTYH010000000
VERSION     DAJTYH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..595
                     /locus_tag="OO609_004140"
                     /pseudo
     CDS             1..595
                     /locus_tag="OO609_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            597..1202
                     /locus_tag="OO609_004141"
     CDS             597..1202
                     /locus_tag="OO609_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6635318.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1189..>1751
                     /locus_tag="OO609_004142"
     CDS             1189..>1751
                     /locus_tag="OO609_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6635319.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIR"
BASE COUNT          437 a          416 c          479 g          419 t
ORIGIN      
        1 ccatccgggg tgaaatactc tccacggtag aaaacgtggt ggatgtttac gtcacccata
       61 atccgaaaaa aacagaacaa aaagccggag tcagtatcca ttaacacccg gttcgcttta
      121 tgttggcgtg tacggcggca gcccggcaga tatcgcggcg gccatctggc gtaaggctcc
      181 gccgggtatt gatatgaatg gcaacacaac gttcactgtt gcagataagg agtacgatcc
      241 gccgtatcct gaatacgtga tcagctggca gacactcaaa cccgtcagtc tgcatgtcag
      301 tgtgacgctg aaaaaaagtg actacctgcc ttcagatatt acccgacagg tacagcaatc
      361 cgtgctggac gcatttaatg gtacagatgg tggtctgcgg gcaagagttg cctctgttgt
      421 ctctgccggg cgctactatg ccggcattta caaaaccgat ccggaacata ttgatattct
      481 gggccttact gtgagccgtg atggttcgtc atggacaacg gctgtcactt tcgggataga
      541 tgagattccg gttctggatg tgtcggatat cagtgtgaaa ctccaggagg cataacgtgc
      601 agaatgtggc tgccactgtg cttgcgcagt atgctgccag cccccgactc aatgccctca
      661 ttaacagctt taacgcagcg ctttcccccg acagttttat cagtgatttt tatggtctta
      721 tctggaacat cgataccgca gaaaagtatg gtcttgatgt ctggggaaag attgtgggtg
      781 tcagtcgctg gctgacggta aaggacgatt ttaattacct gggcttcagc gagtccagga
      841 tggacacccc ggtaatggat gatccctgtc cgtttaatca ggcaccgttt tacaacggaa
      901 aatcggatac ccggactgtt gacctgtctg atgctgtata ccggcggctg atactgatga
      961 aagccatgtc gaacattact gactgttccg ttccggatat taacctgatg ctgagattta
     1021 tgttcggaaa aaaacgccgg gcttatgttc tgaataatgg cgggctgagg atgagttacg
     1081 tctttgagtc cgcgctctcg ttggcagaac tggcgattat ccagtcgtcg ggtgcactgc
     1141 catccccgcc gggtgtttat gtttcagtag ttttaaagga gtcccgtaat gaaggccagt
     1201 gataaacccc gccagctggc ggtccccttt gcgagtaccg gagataaaaa ccgtatcccg
     1261 gacaaggcga cacagcagac cagagagagc ggtaatgctg cgtatgattc aggttttcct
     1321 ccggtgacca tgacagcggt ctcagcggga ggtataccgc cacacggcaa ggattttaac
     1381 ggtctgatgt acgatattac cgcagcaata cggttcgccc aggctggcgg tttgtacacg
     1441 tataatgccg gttttgcggg ggccattggt ggatatgcaa aaggagccat tctcgccgga
     1501 gtcgcaacaa cagcggtctg gctgaatacc acggacgata acctgaccga tcctgaaggc
     1561 tccgacagtg cgggctgggt aaatcttctt gaggatccga aaaggatatt cctgcggcag
     1621 aagaacaatc tgtcagacct tcagaataaa gggacggcac gggataatct tcaggtttac
     1681 agtaaagagc agtcagatca acgctatgtt catcgggaag gcgataaaat aaccggagag
     1741 ctgaaaatcc g
//
LOCUS       DAJTYH010000255         1742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTYH010000255 DAJTYH010000000
VERSION     DAJTYH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(123..953)
                     /locus_tag="OO609_004143"
     CDS             complement(123..953)
                     /locus_tag="OO609_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6635320.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..>1742
                     /gene="intI1"
                     /locus_tag="OO609_004144"
     CDS             1163..>1742
                     /gene="intI1"
                     /locus_tag="OO609_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6635321.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLS"
BASE COUNT          400 a          381 c          415 g          546 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gc
//
LOCUS       DAJTYH010000256         1703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTYH010000256 DAJTYH010000000
VERSION     DAJTYH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..1703
                     /gene="sat"
                     /locus_tag="OO609_004145"
                     /pseudo
     CDS             1..1703
                     /gene="sat"
                     /locus_tag="OO609_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          633 a          292 c          331 g          447 t
ORIGIN      
        1 ttagtcggaa caacccatgg tattgccagc gttaatggtg accaactgac atggataaca
       61 aaatacaatg ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg
      121 aatggcaata atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat
      181 accactggta ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga
      241 aatgttctgt ttaaggataa tttggatttt ggttgcggtg gtattatctt tgacgaaggc
      301 catgaatata acataaacgg tcagagattt acatttaaag gagcaggaat tgatatcgga
      361 aaagaaagca ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata
      421 ggccccggta ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga
      481 aatgttattc ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt
      541 aaggtaatac taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt
      601 actaaacgtg gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc
      661 gccactgacg atggaacaac aataactaac tcagatacaa agaaagaagc cgttctggca
      721 atcaataacg aagactccta catatatcat gggaacataa atggcaatat aaaactaacg
      781 cacaatatta attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc
      841 aacacaaaaa atgatgttga agtcagtaat gccagtctta ccatgtaagg ccatgcaaca
      901 gagcatgcaa tattcagaag cacagcgagt cattgctccc tggtatttct ttgtggaacg
      961 gactgggtca ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat
     1021 tacaaaagta ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt
     1081 aaatttgata cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt
     1141 gaaggaaata tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt
     1201 gataatcttg cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt
     1261 actaatctat ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa
     1321 atagccatag gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact
     1381 cctataagta tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa
     1441 ggtattgata tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact
     1501 gtaacgccgg gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat
     1561 ttcattgcca gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc
     1621 attactctgg gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg
     1681 gcagagactc ttttgtatgg ctt
//
LOCUS       DAJTYH010000257         1690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTYH010000257 DAJTYH010000000
VERSION     DAJTYH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            114..>1690
                     /locus_tag="OO609_004146"
     CDS             114..>1690
                     /locus_tag="OO609_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6635322.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNV"
BASE COUNT          461 a          391 c          428 g          410 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaactac aggtcaaaga cgtcaggacc actgaggcgg
     1261 gaaccgttta tattcatatc aatgaagacg atagtagtct acccggtaag agcatcaaga
     1321 acgctcatag tgaccgctgt gtgcctctgg tcgatggggc ctatggtttt atattggctg
     1381 actttatggc gcttgtagag acccgtagag gcgctgacgg tgatgacgct atggtgttcg
     1441 atggtctgcg tctcatgaag aacggttacg gtgagcaggt gagtaaatgg tttaatcgga
     1501 cgttgttgcc taaagtcctc gtagaccgta gcggcttagc gtttcactcg tttagacata
     1561 cagttgcggc ccagttaaaa caacatgggg tcgagttggc gtatgcacag gcgatcatgg
     1621 ggcatagttc agggtctatt acgtacgacc gttatgctaa agaggtcgag gttgataggc
     1681 tggtcaatgt
//
LOCUS       DAJTYH010000258         1650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTYH010000258 DAJTYH010000000
VERSION     DAJTYH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..564)
                     /locus_tag="OO609_004147"
                     /pseudo
     CDS             complement(1..564)
                     /locus_tag="OO609_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(557..1650)
                     /gene="flhB"
                     /locus_tag="OO609_004148"
                     /pseudo
     CDS             complement(557..1650)
                     /gene="flhB"
                     /locus_tag="OO609_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          423 a          489 c          404 g          334 t
ORIGIN      
        1 cacttcggag cggcgttttt tcgcctcatc ttcaccaatc aatccagcgt taaggtcggc
       61 gtcaatcgcc atctgcttac ccggcatacc atcgagaaca aagcgcgcac ccacttctgc
      121 tatacgcccg gcacctttgg taatgaccat aaagttgatg atcacgagaa tgacaaacac
      181 cacgataccg atagcgaaat tgccaccaac gaggaagtga ccgaacgctt cgaccacctt
      241 cccagccgcc gccgcgccgg tatgcccttc catcaaaatg atacgggttg aagccacgtt
      301 aagtgccaga cgcaacaaca tagtaaacaa cagaatggtc ggaaacgcag caaactcaag
      361 cgtgcgctgg gtaaacatcg ccaccagcaa caccatgatc gacaaggcaa tattgaaggt
      421 aaacaacagg tcgagtatga atgcgggcag tggcagcacc atcatcgaca agatcaacag
      481 gatcaaaatc ggtccggcaa ggatctgcca ttatgtcgat ttcaggtttg cgggcaggcg
      541 cagcatcgcg gccagattac tcatgggtcg gtttctcgtt aataaaatcc agggctgccg
      601 gcaccggaag atgagtaggt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
      661 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
      721 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
      781 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
      841 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
      901 gttattgaca atgacatccg ctttcggcac atcggccatc atccgacgcc gtgcggcagc
      961 tcgctgcatc tgacagatcc gtcctttaac atgggggtcg ccttcgcttt gtttgaactc
     1021 atcacgaata tcctgccgcg acattcgcag ctttttcagg tggctgaaga tttggaaaaa
     1081 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagccctac
     1141 caaatccatc gcattaccca tggcggtaat cggtgactcg gccattaagc gcatcatctg
     1201 cggccagtga tgccagagaa aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     1261 tgccttaagc aactccgcgc tagtctgtgc cgagaatatc cgtttaatgc ccggtagcgg
     1321 gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc cccccagcat
     1381 aaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggtagca gcgccagcat
     1441 ggcttctctg atcagcagaa taatttgccc gaggatcaga ttcgggtctt tgataatact
     1501 gtgatcaaaa tgcagcccag cggagagcat gcccgacaat cgacgggcca gcgacacacc
     1561 gccaaaccag ataacactaa cgcccaccag caaaatcagc agtgaggtca gttcacggga
     1621 acgcgggatt tgcccctctt cccgcgcttt
//
LOCUS       DAJTYH010000259         1619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTYH010000259 DAJTYH010000000
VERSION     DAJTYH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..221)
                     /gene="flhe"
                     /locus_tag="OO609_004149"
     CDS             complement(<1..221)
                     /gene="flhe"
                     /locus_tag="OO609_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6635323.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVR"
     gene            complement(221..>1619)
                     /locus_tag="OO609_004150"
     CDS             complement(221..>1619)
                     /locus_tag="OO609_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR6635324.1"
                     /translation="RAATGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGGIVTRV
                     STDQDVGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRG
                     REQKAPAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRI
                     RSIRKKFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTA
                     AGTLPGEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAEL
                     FGRQEAQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETL
                     AEHAPIQSDPHELSAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGG
                     LEPGLADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLEL
                     SDNRHIRMTATIGGK"
BASE COUNT          369 a          498 c          403 g          349 t
ORIGIN      
        1 aagcgaaccc gcagtcctgc aggtgtcggg ccgataagct gataacgcca cgccaccagc
       61 gtcatcaatc ctgaagcagg ttgtcgcgca gaaagaggtg cagacgacat cgactcaccg
      121 cgatgattta gcgtaattcc gacactactt gcctgccaca tcccctcccc ggcggcttgc
      181 accagcagcg gaaacaataa tatcgctaat aaggttctca tcatttaccg ccaattgtcg
      241 ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt cgacaggacc actaactgcg
      301 gcaagctgcg gcgcaggaag cgagacaata atggtcgcag cgcgtggttc accaacaata
      361 ctggcggcgc acccagcatc tcctgacggg atagagcttc ctgagtttgc gccagtaaac
      421 gatccgccag ccctggctcc agtcctcccc cgccctgcag cgcctgtagt aacaaacgtt
      481 ccagcggtgt atcgaggcca ataacatgga cttcatcttt gccaggaaac cactgctggg
      541 taatcgcccg tcccaacgcc acgcgcacga cggcgcttaa ttcatgtgga tcgctttgga
      601 tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat atcgcgaatc ggtacttttt
      661 catcgaggag attttgcagc actttatgca gtgtggtgag cgtgacgacg ccaggaacga
      721 gatcttccgt cagctttggc atctcctggg cgacgcgatc caacagctgt tgcgcctcct
      781 gacgaccaaa cagctctgcg gcatgctggc taatgaggtg gttaagatgc gttgctacca
      841 ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc ctgttctttt agcgcacttt
      901 caatccagat agcattcagg ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc
      961 cggcagcggt tccagggtta atcgccagcc agcgccccgg ataagcatca ccactgccaa
     1021 tctccacgcc tttcatcaaa atgcgatagc gggcaggttg cagatccata ttgtcgcgaa
     1081 tgtgcaccac tggcggcaga aatcccatct cctgggcaaa tttcttgcgg atactgcgta
     1141 tacggcccaa caactcacca tcctgctgga aatcgaccat cgggatcagt cgataaccca
     1201 cttccattcc cagagaatct tccagttgta catcgttcca cgtcgcttcg acaacggtat
     1261 tattctctgc catttttacc ggtttgggtt cggcaggcgc tttttgttcg cgtccgcgta
     1321 tccaccaggc cagccccagc aatccggcag tgaacagcaa aaataccagg ttcggcattc
     1381 caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa caacataacg cttgggttac
     1441 tgaaaagctg attcaccatc tgctcgccaa catcctgatc ggtgctgaca cgcgtaacga
     1501 tgccccccgc ggcggtagaa atcaccagcg ccggaatttg tgccaccaga ccgtcgccaa
     1561 tggtcaatag cgtataactt tccgccgcgt gtcccatgct catgccagtt gcagcacgc
//
LOCUS       DAJTYH010000260         1596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTYH010000260 DAJTYH010000000
VERSION     DAJTYH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..169
                     /locus_tag="OO609_004151"
     CDS             <1..169
                     /locus_tag="OO609_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6635325.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            154..792
                     /locus_tag="OO609_004152"
     CDS             154..792
                     /locus_tag="OO609_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6635326.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..1444
                     /locus_tag="OO609_004153"
                     /pseudo
     CDS             1020..1444
                     /locus_tag="OO609_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1439..>1596)
                     /locus_tag="OO609_004154"
     CDS             complement(1439..>1596)
                     /locus_tag="OO609_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6635327.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          443 a          306 c          409 g          438 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttc tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg actctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tgcaggaaaa cgcctgaggg gctggttgcc tgtagcctgc
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata agatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagtgggg gggtgcccct attaagttgt caagcatgtt
     1441 atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga acgtcgcagc
     1501 gtcgtctcgc cagggcgata agcgcctgat tgtgtcgttt tccctgactc attttgcggg
     1561 tgtagtaagc cctggagagc ggatccctga gcgcgg
//
LOCUS       DAJTYH010000261         1528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTYH010000261 DAJTYH010000000
VERSION     DAJTYH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..360
                     /locus_tag="OO609_004155"
     CDS             <1..360
                     /locus_tag="OO609_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6635328.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO609_004156"
     CDS             361..726
                     /locus_tag="OO609_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6635329.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO609_004157"
     CDS             723..1406
                     /locus_tag="OO609_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6635330.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          435 a          314 c          418 g          361 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtga
//
LOCUS       DAJTYH010000262         1522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTYH010000262 DAJTYH010000000
VERSION     DAJTYH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..1321)
                     /gene="treF"
                     /locus_tag="OO609_004158"
     CDS             complement(<1..1321)
                     /gene="treF"
                     /locus_tag="OO609_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR6635331.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVR"
BASE COUNT          356 a          392 c          407 g          367 t
ORIGIN      
        1 tgcgtacggc gtttgccaga cgatcggcct gttcatggtt cgccatgccg acatagagcg
       61 gcacaatggc ggcagcggaa aacagcgcca gttgttcgcg tcgccagtcg taatcgcggt
      121 agatgccgtt ttcatcatcc cagaggtaac ggtttaccgc atcgcgacgg gcactggcct
      181 tctggcggaa cagcgcttct gtctctttct cgcctttcag cgccgagatg ttggcgatgg
      241 cgctctccag tttaaacagg aaggcattca gatcgatggg gatgaactgg gtggtacgaa
      301 tgctcgccag acgaccagta tcacgcagcc aacgggaaga gtaatcccaa ccggaggccg
      361 ctcccgcgcg taaatcgcgg tacacctcgt tgggcgggcg accagaatgt ttcgcggttt
      421 caacgtcctc aagccaggat tcgtcacgcg gcgtgtcgcg atcgtcccag taacggttga
      481 gcagcgatcc gtccggcatc cgcacaacat ggcgataggc ctgattaggg atcaacgatt
      541 ctgcaccgtc catccagaag gcatattcca ttttaaggtg gtcgagatag cggcgcgcac
      601 cgcgtacacc atcttcttca aacaactcca ccatcagcgc aaaaaccggt ggttgcgagc
      661 ggctcaaata ataggtgcgg ttgccgttgg ggatgtgacc gtagttttcg atcatccagg
      721 cgaagttatc ggccatgcat ttcagcaaat cttcccgacc actttccgcc agtcccagca
      781 tggtgaaata ggaatcccaa tagtaggttt cgctaaaacg gccgcccggg acaatatatg
      841 actgcggtag cgccagcaga gaagaccacg gaatgtgatc ctgtggttcg cgggttagca
      901 ccggccacag ctggtcgata tgctctttca gggaattttg cgggtccgat acatactcgc
      961 tggagtagac ctccggcagc cagaagtgat tttcaacaaa cttgcgcaag tcaaaatcac
     1021 gatgacggcg cactttacgg tagcggatta aaatatccag cgggtccatt ttgggtgcgc
     1081 agtcgggaaa ggttttactg tcggggaaaa ttttcgccga ctgaacatgc tcgaacagtt
     1141 cgagatagcg atcggcaggc gtcagcgcat cagaggcggg cagcccttca atcatctcgg
     1201 gttccggttc tgcctcgatc atctcatcca gttttaattc atacgggtcc agttcatagc
     1261 agagatcgac ctcgatcatc agttcgtctg gattagggtt ttgaattttc tgattgagca
     1321 taaggatcca acctccgaaa ttcgtcgcta attatcgggc ttttctttaa gggtagtcat
     1381 tcgctccgcc tggtgggcga caaagcgtgc ggtggatcac gtttattaac ttgtcaaaaa
     1441 atataacgca ctaatatcaa tggaatttat aaaaatacgg atttggtgtt gtttttcatt
     1501 ttcagaacat tcaatcctgc tc
//
LOCUS       DAJTYH010000263         1505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTYH010000263 DAJTYH010000000
VERSION     DAJTYH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(100..567)
                     /locus_tag="OO609_004159"
     CDS             complement(100..567)
                     /locus_tag="OO609_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6635332.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(633..872)
                     /locus_tag="OO609_004160"
     CDS             complement(633..872)
                     /locus_tag="OO609_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6635333.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1007..>1505)
                     /locus_tag="OO609_004161"
     CDS             complement(1007..>1505)
                     /locus_tag="OO609_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6635334.1"
                     /translation="GYRFLLLLLQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRK
                     ALDVAGLSSSLIKTIPRKGVRYLGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDAN
                     IPENDNSQEFSTEELLPIKHTDKNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLF
                     LNQDK"
BASE COUNT          484 a          333 c          268 g          420 t
ORIGIN      
        1 gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta ctgagagaaa
       61 aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca atgggaaaac
      121 aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac cgttttcacg
      181 aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc aaaaggtcat
      241 tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa cagttaacag
      301 cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca cgagcaaaac
      361 gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca aaaaaataag
      421 cacggttaac accagtaaat aacgatggct aataattctg agtaatatat ccccccatga
      481 aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc atattgctgc
      541 cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt tacgtctgac
      601 agcctctaga gagttttttt attttttata agttacggat tactcgttcc ggaaatcgtt
      661 acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc caggaagcct
      721 gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc aatcaatgca
      781 tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca ctgtttagtt
      841 ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat tatttaaatt
      901 caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat gtggctataa
      961 ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat ttatcttgat
     1021 taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct aacacggtaa
     1081 gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt ttgtcagtat
     1141 gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc tcaggaatgt
     1201 ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta ctgtcttcat
     1261 cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat ctgacgcctt
     1321 ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat gcctttctaa
     1381 gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc tcccgccaaa
     1441 cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt aaaaatcgat
     1501 agccg
//
LOCUS       DAJTYH010000264         1488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTYH010000264 DAJTYH010000000
VERSION     DAJTYH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            240..509
                     /locus_tag="OO609_004162"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO609_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO609_004163"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO609_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO609_004164"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO609_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6635335.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          434 a          296 c          356 g          402 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaa
//
LOCUS       DAJTYH010000265         1474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTYH010000265 DAJTYH010000000
VERSION     DAJTYH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(5..184)
                     /locus_tag="OO609_004165"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="OO609_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="OO609_004166"
     CDS             708..992
                     /locus_tag="OO609_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6635336.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="OO609_004167"
     CDS             992..1267
                     /locus_tag="OO609_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6635337.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          402 a          368 c          361 g          343 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatctcaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga catt
//
LOCUS       DAJTYH010000266         1457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTYH010000266 DAJTYH010000000
VERSION     DAJTYH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO609_004168"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO609_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6635338.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO609_004169"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO609_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1457)
                     /locus_tag="OO609_004170"
                     /pseudo
     CDS             complement(1292..1457)
                     /locus_tag="OO609_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          450 a          224 c          317 g          466 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcct
//
LOCUS       DAJTYH010000267         1443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTYH010000267 DAJTYH010000000
VERSION     DAJTYH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            82..1275
                     /locus_tag="OO609_004171"
     CDS             82..1275
                     /locus_tag="OO609_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6635339.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="OO609_004172"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="OO609_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          422 a          313 c          339 g          369 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DAJTYH010000268         1440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTYH010000268 DAJTYH010000000
VERSION     DAJTYH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..892
                     /gene="arsB"
                     /locus_tag="OO609_004173"
     CDS             <1..892
                     /gene="arsB"
                     /locus_tag="OO609_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6635340.1"
                     /translation="GTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREYAS
                     VMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLL
                     VGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVV
                     YGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGS
                     TASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIM
                     TLPVLFVTLAALALRLSFTL"
     gene            905..1330
                     /gene="arsC"
                     /locus_tag="OO609_004174"
     CDS             905..1330
                     /gene="arsC"
                     /locus_tag="OO609_004174"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6635341.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
BASE COUNT          305 a          369 c          390 g          376 t
ORIGIN      
        1 aggcacgacg ctggcgttcg tgatggcggc cggattcatt gccgataccg ccagcctgcc
       61 gcttattgtc tccaacctgg tgaatatcgt ttccgctgat ttctttggcc tcggctttcg
      121 cgaatacgcc tcggtgatgg tgccggtgga tatcgccgcg attgttgcca cgctggtgat
      181 gctacatctc tattttcgca aagatattcc gcagaactac gatatggcgc tgctgaaatc
      241 tcccgcagaa gcgatcaaag atcctgctac gttcaaaact ggctgggttg ttttactgct
      301 tctgctggtg ggatttttcg tcctggaacc gctcggcatt ccggtgagcg ccattgcagc
      361 tgtgggcgca ctgatattat ttgtcgtcgc taaacgcggt catgcgatta atacgggtaa
      421 agtcctgcgc ggtgccccct ggcagattgt catcttctcg ctcggcatgt atctggtggt
      481 ttatggcctg cgcaatgccg gattaacgga atatctttct ggcgtactca acgtgctggc
      541 ggataacggc ctgtgggccg cgacgctcgg caccggattc ctcaccgcct tcctctcttc
      601 tattatgaac aatatgccga cggtactggt tggcgcgttg tccattgatg gcagcacggc
      661 atctggcgtt atcaaagaag cgatggttta tgccaacgtg attggctgcg atttggggcc
      721 gaaaattacc ccgattggta gcctggcaac gctgctctgg ctgcacgtac tttcgcagaa
      781 gaatatgact atcagctggg gatattactt ccgtacaggg attatcatga ccctgcctgt
      841 gctgtttgtg acgctggctg cgctggcgct acgtctctct ttcactttgt aatgagatac
      901 tgatatgagc aacattacca tttatcacaa cccggcctgc ggcacgtcgc gtaatacgct
      961 ggagatgatc cgcaacagcg gcacagaacc gaccattatc cattatctgg aaactccgcc
     1021 aacgcgcgat gaactggtca aactcattgc cgatatgggg atttccgtac gcgcgctgct
     1081 gcgtaaaaac gtcgaaccgt atgaggagct gggccttgcg gaagataaat ttactgacga
     1141 tcggttaatc gactttatgc ttcagcaccc gattctgatt aatcgcccga ttgtggtgac
     1201 gccgctggga actcgcctgt gccgcccttc agaagtggtg ctggaaattc tgccagatgc
     1261 gcaaaaaggc gcattctcca aggaagatgg cgagaaagtg gttgatgaag cgggtaagcg
     1321 cctgaaataa agcggcgata tccactgccg atagttggta aagtgcgctt tgtttatgtc
     1381 ggatgcgacg ctaacgcgtc ttatccgacc tacaaaaccg tgcaaattca ggtaatgctg
//
LOCUS       DAJTYH010000269         1426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTYH010000269 DAJTYH010000000
VERSION     DAJTYH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            190..492
                     /locus_tag="OO609_004175"
     CDS             190..492
                     /locus_tag="OO609_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6635342.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            483..965
                     /locus_tag="OO609_004176"
     CDS             483..965
                     /locus_tag="OO609_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6635343.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            1033..1426
                     /locus_tag="OO609_004177"
                     /pseudo
     CDS             1033..1426
                     /locus_tag="OO609_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          383 a          296 c          352 g          395 t
ORIGIN      
        1 gcatttcatt atcaaaatca cattaaacgg ccgtaatcag acatgatttg tgcgccgaca
       61 cagatcatcg tcacaatgtt caggtctctg ccattgtttt tatcagggga tcgcttttcc
      121 cgtaagtgat tctgtatagc gttctgttac catttgcgat aacattaatt gcatagcaaa
      181 ttgttaccaa tggaggtttt tatgtctaca gctgcaagcg tccgtaaaac accgcgagaa
      241 catcagatta acatccgggc aactgatgag gaacgagccg ttattgatta tgctgcgagc
      301 ttggtcaata agaaccgtac cgatttcatc atggaactgg cgtaccagga agctaaaaac
      361 attatcctgg atcaacgttt atttgtgctg gataacgaac gttatgattc gtttattacg
      421 caactggaag ctcctgtgca gaatgcagaa gggcgtgaac gtctgatggc ggtaaagcct
      481 gaatggaaat aaatgtcacc gcgccagcat tgttgacgga tgagcatata cttcagccat
      541 ttgactgtgg aaatgaggtg ctaagtaact ggttacgtgg tcgggctatg aaaaaccaga
      601 tgctcaatgc ttctcgcacg ttcgttattt gcttggagga tactttacgt attgtgggat
      661 actactcatt agctactggt tccgtcactc atgccgagct tggtcgcagt ttgcggcata
      721 acatgcccaa tcctgtccct gttgttctgt tagggcgtct ggctgttgat gtttgtaccc
      781 aggggcatgg tttcggtaaa tggctactaa gtgatgccat tcaccgggtt gtcaatctgg
      841 ctgatcaggt tggcattaag gctgttatgg tacatgcaat tgatgatgac gccagagcat
      901 tttatgagcg ttttggtttt gttcagtcag ttgtggcacc taataccttg ttttataaag
      961 tctgagtctg aggtagcctg agtttaacgg acactccttc ctgaaataga atggcatcag
     1021 aaggagccaa taatgagcag aaaaacccaa cgttactcta cggagtttaa agccgaagcc
     1081 gtcaaaacgg ttcctgaaaa tcagctttcg atcagtgaag gagcttcccg attatctgtt
     1141 cctgaaggca ctttaggaca atgggttacc gccgcaagaa aagggctcaa tacaccaggc
     1201 tcccgcacag tggctgagct ggaatctgaa gttatgcaac tgcgtaaggc tttaaatgaa
     1261 gcacgacttg agcgagatat attaaaaaaa gcaacagcgt attttgcaca ggagtcgctg
     1321 aaaaatacgc gttagtcgaa caatggcgac aacaattccc tgttgaagcg atgtgtcggg
     1381 tacctggtgt atccaggagc ggttattacg accgggtaca gcatgc
//
LOCUS       DAJTYH010000270         1410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTYH010000270 DAJTYH010000000
VERSION     DAJTYH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..170
                     /locus_tag="OO609_004178"
     CDS             <1..170
                     /locus_tag="OO609_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="regulator for the transport and utilization of the
                     aromatic beta-glucosides arbutin and silicin; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bgl operon transcriptional regulator BglJ"
                     /protein_id="HCR6635344.1"
                     /translation="TQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMR
                     HCEASNVLHPY"
     gene            complement(208..996)
                     /gene="fhuF"
                     /locus_tag="OO609_004179"
     CDS             complement(208..996)
                     /gene="fhuF"
                     /locus_tag="OO609_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6635345.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            1137..1373
                     /locus_tag="OO609_004180"
     CDS             1137..1373
                     /locus_tag="OO609_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6635346.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          356 a          312 c          374 g          368 t
ORIGIN      
        1 tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg cataaattta
       61 atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc gaagatattc
      121 tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa tccgcatgat
      181 gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac attgctgcac
      241 atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt cgcgcagtac
      301 cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa aaaagagggc
      361 atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag tgagatacca
      421 gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc cggtcgcttc
      481 tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc gctgctgcgg
      541 cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg cgacgcgtcc
      601 ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt tttcctgcgt
      661 cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg cccatagtga
      721 gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat gatcggaata
      781 gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg tcatggcgtt
      841 aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat gtttggcgat
      901 catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg ttcgccagat
      961 gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa tctaaatgat
     1021 aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc tggcttgcaa
     1081 atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat gaaaccatgt
     1141 tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg attgcagggc
     1201 tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg ggattagtat
     1261 tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta ccatcgtgcc
     1321 tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga tgacaaaaca
     1381 gggaagaaac aagaaaggaa gtaaagataa
//
LOCUS       DAJTYH010000271         1371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTYH010000271 DAJTYH010000000
VERSION     DAJTYH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            326..>1371
                     /locus_tag="OO609_004181"
     CDS             326..>1371
                     /locus_tag="OO609_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6635347.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          448 a          300 c          219 g          404 t
ORIGIN      
        1 tgaagttact atgtatataa gcttatttta tacatgatta ttagcaacaa atattaccaa
       61 gtcaagtagt caggctggct aaaatagttg gagatgttgt agtatcttac tcaagactaa
      121 tgtcttctgg tataaatcct ctgcattgtg aatatgtaaa acatcaaaaa aagatgaacc
      181 aaatatacga aaaaagcaaa tatacttacc agggtagaaa gcatgatata acactcattc
      241 ccttatggca aatattctga aacaccctcc tcattaggag agatacatta aatacaattt
      301 attcacaatc taattccata tccatatgcg agaaattaat atgctcagaa acatttcatc
      361 ctgtttattt ccacatatca gcacaattac atcccccaac cattatttgt ccgaatggga
      421 tgattgggag aaacaggggt taccggaaga acagcgtact gaggcggtaa gaagacttcg
      481 tgcatgtctt acctctaagg ggcataaact ggacctgcga gccttggcgc tttcctcgct
      541 acctgtactc cctgcttgca ttaaaaagct tgatgtgagc tgtaataaat taaccatcct
      601 tactgattta cctgaaaata ttaaagaact tattgcaaga gataatttct taacacatat
      661 atctgcatta ccacattatc taataacttt ggatgtgtcc gaaaatcaat tagagaatct
      721 gccgttatta ccagacacca tcaaatcact aagcgcagag tataataggt tatccacact
      781 gccttcatta cccttgaatt taaaaaaact tgaggttagg aacaacgaac tgcaaactct
      841 tccatctctg ccttctaatc ttaagatact taaggttgcg cacaaccatc ttactgaact
      901 gcccccttta cctaggagac tgcaacttct ttttgcatat agcaatagat taagcaactt
      961 accaaacatc caagaaaata ttatcatgag aagatttttt tattttgaaa acaaccaaat
     1021 aactacaatc ccgacaaatc tttttcgttt agatcctcat ataactattg agattgcaaa
     1081 taacccctta tcagatcaaa ctctgctatt cttaatacag caaacttcgg ttccaaattt
     1141 taacgggcct cagtttcgta tttccctgtc agaccaaaac agactgtttt tacgccagat
     1201 gttgccgcaa aatttacatt cgcgccatat cagagtcatc actgaagggg ggcagaactt
     1261 tcagatcccc cctcttcccg aaactgtggc agcctggttt cctgaagcag atcgtcggga
     1321 ggtttctaca caatggactt ctttttccac cgaggagaat tcccgggcat t
//
LOCUS       DAJTYH010000272         1367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTYH010000272 DAJTYH010000000
VERSION     DAJTYH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(187..771)
                     /gene="kptA"
                     /locus_tag="OO609_004182"
     CDS             complement(187..771)
                     /gene="kptA"
                     /locus_tag="OO609_004182"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6635348.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1021..>1367
                     /locus_tag="OO609_004183"
     CDS             1021..>1367
                     /locus_tag="OO609_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635349.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPV"
BASE COUNT          316 a          351 c          311 g          389 t
ORIGIN      
        1 ggtatgaccg gaagcgacgc tgattccagt ccccaactga ccgcctgccg taaatcactt
       61 taatttcagc agttgttatt aaaggatacc cttttacgag ggtatccctt cagactgata
      121 acaaacgcta cattgccgga tgtgctgcgc ttaccaggcc tatataaccc ctgcaatata
      181 ttgaatttat tgtttttgta ccccagtcgg cataaacgac cactcaagaa actcaacagc
      241 aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc
      301 catttcctgt gctttgacgg ttaagacaac cggcgatccg tgtcttgctc ccactttgcg
      361 tgccgttgct tcatcagcag agagatgcac gtaatgacga tcccctgcaa tcagcccctg
      421 tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact gtggcggtgt
      481 tttttcggca aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca
      541 cctaccatca ctggaataac taaaacgctt tttatcgctg gttgcgacca cggtatccag
      601 caatgcacgg gttaatcgct tgcccgcttt ttgggcacaa agtatgagct tattgatatc
      661 cgcccatcct tcacggtcca gtacgatacc aatcgcctcc ggtttgtgtc ggaggacaaa
      721 acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca tcgcgttttc
      781 gtccctggat tttctgtttt tatttactaa tgtcattgtc actgggttta tgaattttct
      841 gcaactcaat cctgcgcaga accttttcca cacagcgtaa tttttcccct atgcaaaaaa
      901 cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata aggtttttca
      961 gttccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt
     1021 atgacacaac aaggggatgc tgtagccggg gagctggcga cagaaaaagt gggcattaaa
     1081 gggtatctgg ctttttttct caccataatc ttttttcccg gtgttttttc cggaacagac
     1141 agctggtggc gcgtttttga tttttccgtt ctgaacggat cgtttgggca actgcctggt
     1201 gcgaatggag ccaccacctc atttcgcggg gtgggtggcg cgggggctaa agatggcttt
     1261 ctctttgcgc tggagctggc accgtcagtc attctctctc tggggattat ttcaattacc
     1321 gatggtcttg gcggattacg cgccgcgcag cagttaatga cacctgt
//
LOCUS       DAJTYH010000273         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTYH010000273 DAJTYH010000000
VERSION     DAJTYH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.282"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            400..1149
                     /locus_tag="OO609_004184"
     CDS             400..1149
                     /locus_tag="OO609_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6635350.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          387 a          278 c          275 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAJTYH010000274         1362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTYH010000274 DAJTYH010000000
VERSION     DAJTYH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            109..1029
                     /locus_tag="OO609_004185"
     CDS             109..1029
                     /locus_tag="OO609_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6635351.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1029..1362
                     /locus_tag="OO609_004186"
                     /pseudo
     CDS             1029..1362
                     /locus_tag="OO609_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          383 a          307 c          332 g          340 t
ORIGIN      
        1 ttaaatgata ggtctaatca tgatttaggg tctaatcaac ttaatgatca atatctatag
       61 tgatatcacc aaccgtcaaa acgaaccgac aactccatga ggaactgaat gatgaataat
      121 aaagtcagct tcactaacag caataatcca accatctctt tgtctgcagt gatctatttc
      181 cctccaaaat ttgatgaaac ccgtcagtat ccggcaattg tggtatctca cccaggtggg
      241 ggcgttaaag agcaaacggc cggaacctat gccgaaaagc tggcggaaaa gggatttgtc
      301 actattgctt atgacgcatc ttatcaaggt gaaagtggcg gcgagccgcg tcagttagaa
      361 aacccataca ttcgtactga agacgtcagt gccgtgattg attacctgac aacgctttct
      421 tatgtcgaca atacccgaat tggtgcgatg ggaatttgtg ccggtgcagg atatacagcc
      481 aatgctgcaa ttcaggatcg ccgcatcaag gccatcggta ccgttagtgc ggtcaatatt
      541 ggttcgatgt tccgcaatgg ttgggaaaac aatgtgaaat ctattgatgc tctgccacac
      601 attgaagctg gctcaaacgc ccgaaccagc gatattagca gcgaagaata cgccattatg
      661 ccactggctc caatgaagga ggccgatgcg ccgaacgaag aactgcgtca ggcctgggag
      721 tattaccaca cacctcgtgc gcagtatcca acagcaccgg gttacgctac tttgcgcagc
      781 cttaaccaga ttattaccta tgatgcttac catatggcgg aagtgtacct gactcagccg
      841 atgcagattg tggcgggcag ccaggcaggg agcaaatgga tgagtgacga cctgtacgat
      901 cgggcatcaa gccaggataa acgctatcac attgttgaag gcgcaaacca tatggatctt
      961 tatgacggca aagcctatgt ggctgaagca atttcagtat tagctccgtt ctttgaggaa
     1021 actctgtaat gaaacatatt caaatccgca atagtgatat ggcctggcat attgctgcca
     1081 atattcagtt tccaccaaat tttgatgagt caaaacagta tccggcgatt atcagcgtcc
     1141 atccttttgg cagttgtaag gagcagacgt ctggcaatat ctatggcaag gcattggctg
     1201 aaaaaggcta tgtcgttctc gcttatgacg ccagtttcca gggagaatct ggtgggtagc
     1261 ctcgttggat agaagatccg acacagcgcg tagaggatat tagccgtgtt atcgattatg
     1321 cagtaactct gccatatgtt gatgctgaac gcattggcgt gc
//
LOCUS       DAJTYH010000275         1341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTYH010000275 DAJTYH010000000
VERSION     DAJTYH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            102..468
                     /locus_tag="OO609_004187"
                     /pseudo
     CDS             102..468
                     /locus_tag="OO609_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            396..869
                     /locus_tag="OO609_004188"
                     /pseudo
     CDS             396..869
                     /locus_tag="OO609_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          325 a          337 c          407 g          272 t
ORIGIN      
        1 tctcatggcg gttatggggc ttgccggtgt tctccggggt aaaaaggtcc acactaccgt
       61 cagccggaaa gccgttgccg caggcgaccg cgtaaaccgt catcaaggca atgtgccgcg
      121 tactccgggt ggcccgcagc ggctggtata cgtggtgtca gcggcggaca aggataaaca
      181 cacgtccgca gttccgtcag cactgcgaca gcgttgtccg caaggctttt acccagtcca
      241 gcgttacggt gccccacgcc tgacggatga actgtgtgct ctggttacca ctttaacgta
      301 aaaaccgtgg cagccagtct gcgccgtcag gaactgagcg caaaagcctc ccagaagttc
      361 agcccgatca gttaccgtgc acacggcctg cctgtatcag aaaatctgct gacgcaggat
      421 ttttacgcca gtggcccgaa ccagaagtgg gcgggagaca tcacgtacta ttacagctca
      481 ccgactgcgg ggaaacacgg ggcgccgggt tattgaaggt gacctgtcca gctacttcga
      541 cacagtacat caccggctgc tgatgaaagc cgtctgccgc aggatcagtg atacacgttt
      601 tatgagactg ctgtggaaaa cgccgtgttg atgttggtct cttccgggcg gtcagtgaaa
      661 gtatgccaca gggcggtgtg atctcgccgt tgctgtcgaa catcatactg aatgagttcg
      721 accagtatct gaataaacgc tacctgagtg gaaaagccag aaaggatcgc tggtactgga
      781 atcacagtat ccagcgaggc cgaagtactg ctgtcaaaga aaactggcaa tggaaacctg
      841 ccgttgctta ctgttgctat gccgatgact gtgtgccacg aaggcgcgtc ctggggacgt
      901 gagtccctgt gcagccatgc agcacaagag atgagggaag gccccttggt gtcggcgtgc
      961 aggcgcaggc accaaaccat ccactgctgc tgcatttaag agatgtggtg gtgagcgaga
     1021 tgggaaaagg tcggacgtga gccgatcagg tatggttcga agagacgaac gaaagtgaac
     1081 cgctgatgat gtgtcgaaag catatagatg atgtcaaaac aatggtggag tcgttcctgt
     1141 gggatatgtt tactgcctgc gcggcatccg gcataaaagc ggcgtgaagt cgattcaggc
     1201 tcttgtgggg aacgtggacc tgtcaccccg atgtgaaggg agaagcgcaa gtggaagccc
     1261 catgagcgcc agagtaccaa tgcggggtac agggacggag caccaagcga ttgcgcagta
     1321 aacatgcttg acaacttaat t
//
LOCUS       DAJTYH010000276         1317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTYH010000276 DAJTYH010000000
VERSION     DAJTYH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..428
                     /locus_tag="OO609_004189"
     CDS             <1..428
                     /locus_tag="OO609_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635352.1"
                     /translation="PDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAID
                     KALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNPK
                     THKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            548..721
                     /locus_tag="OO609_004190"
     CDS             548..721
                     /locus_tag="OO609_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6635353.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            821..>1317
                     /locus_tag="OO609_004191"
     CDS             821..>1317
                     /locus_tag="OO609_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6635354.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEE"
BASE COUNT          341 a          321 c          329 g          326 t
ORIGIN      
        1 gtcctgacac aaacgccgcc actgtaaatc agacacttaa atgcgactca aaagcccggg
       61 ctgaccagcc tggtgcatgt ccaacaacgt acgagttata cgaaggtgac gctacctaca
      121 gagctgctat cgacaaagca ttaaaccctg ttgggttaaa cggaatgttt ggggaagacg
      181 gctatatgga tggtcctgat ggtggtgcgt atcctgtaaa cattaacgga acgacctggg
      241 tagaaggtgg cggttgtaaa gcccacgcct gcggctggga ctatatcgta acactctata
      301 acccaaaaac gcataaagtc gttggttact attacaacat cgaccctggt tacctgattt
      361 ggtttggtga aactggcgtt cacgaattcg cgtacctggt cagggactac gtaaacaaaa
      421 caaattaaag gcgtaagtta tgcggttcat cttcgcgtta ttaatttatt gatatcaaaa
      481 gtaaatacgg ctctgccatg atgacaggga cttatatttt cagggaacca aatacaggtc
      541 tggttgtatg tcacgtttat tgcccgaagt ccctttctca cgcgagcagg ctgttgtcat
      601 cacaacagct taccgcaatg tgtttatcga cgatgaccag ggaactcatt tccgtcgggt
      661 tatccgcaac gcagagactg acgccggaag gttcgccagt gacggtcttc tcagacaata
      721 aacctcatta atttcgtcca tcaggccgcg tcttctccgg gagacgtggc tttttcattt
      781 attacaccag taaatcttaa ccaccgataa ggagcaaatt atgcgactag ccagtcgttt
      841 tggttatgct gcaaaccaga tacgccgtga ccgtccgctg acacacgaag aactgatgca
      901 ctatgttccc agtattttcg gtgaagaccg gcatacttcc cgcagtaaac gctatgcgta
      961 cactcccacc atcaccgtac tggaaagcct gcagcgggaa ggctttcagc cattcttcgc
     1021 ctgccagaca cgtgtgcgtg acccgggtcg ccgggaatac acaaaacaca tgctgcgtct
     1081 gcggcgggcc ggacagataa ccggtcagca tgtccctgaa attattctgc tcaactctca
     1141 tgacggttca tccagctacc agatgttacc cggatatttt cgtgccattt gtaccaatgg
     1201 actggtctgc ggtcagtctc tgggagaatt gcgtgttcca caccggggaa atgtggtgga
     1261 gaaagttatt gaaggggctt acgaggtggt gggcgtgttt gaccggatag aggagaa
//
LOCUS       DAJTYH010000277         1294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTYH010000277 DAJTYH010000000
VERSION     DAJTYH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..265)
                     /locus_tag="OO609_004192"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OO609_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO609_004193"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO609_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6635355.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1171..1294)
                     /locus_tag="OO609_004194"
                     /pseudo
     CDS             complement(1171..1294)
                     /locus_tag="OO609_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          379 a          320 c          206 g          389 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gtgtctaaaa
     1141 agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca ggactaacag
     1201 ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac cgccagtttt
     1261 agtgttgtac gaataccaga gccagtgttg ttgc
//
LOCUS       DAJTYH010000278         1265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTYH010000278 DAJTYH010000000
VERSION     DAJTYH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            24..425
                     /locus_tag="OO609_004195"
     CDS             24..425
                     /locus_tag="OO609_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6635356.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            437..808
                     /locus_tag="OO609_004196"
     CDS             437..808
                     /locus_tag="OO609_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6635357.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            795..>1265
                     /locus_tag="OO609_004197"
     CDS             795..>1265
                     /locus_tag="OO609_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6635358.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          282 c          392 g          288 t
ORIGIN      
        1 tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa cgtctgagag
       61 aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca gatattgcgc
      121 agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac atccgggata
      181 agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata tcagcgcagc
      241 cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg gtgacgctgc
      301 ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg ccgggaatgg
      361 cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg gccagaatgc
      421 aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac ttgatctcgc
      481 agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag gccggctaaa
      541 agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg tggccggcgg
      601 tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata ttttacggct
      661 gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc gtatcacccc
      721 tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg gaaacgtaag
      781 ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac agccttgata
      841 cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag aaagcggtct
      901 cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag gtgaaaggga
      961 tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg tcaggaaaaa
     1021 tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg aacacaacac
     1081 ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg gcatttgttc
     1141 agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg cgttttgcaa
     1201 cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag gtagtgaaaa
     1261 tcccg
//
LOCUS       DAJTYH010000279         1242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTYH010000279 DAJTYH010000000
VERSION     DAJTYH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..135)
                     /locus_tag="OO609_004198"
     CDS             complement(<1..135)
                     /locus_tag="OO609_004198"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6635359.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     E"
     gene            complement(329..>1242)
                     /locus_tag="OO609_004199"
     CDS             complement(329..>1242)
                     /locus_tag="OO609_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6635360.1"
                     /translation="DRVSDVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWP
                     FEHICTLLRELVKHSPGDFGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSVGIV
                     WRRAAPTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQ
                     QKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITL
                     IVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDTITRNHQCRSM
                     WQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          298 a          322 c          308 g          314 t
ORIGIN      
        1 ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga aacgctcatg
       61 ccgggaacaa ctacggccat gtcgataaac ttcatcggaa ttacagcgcg gacaatgaac
      121 agtaacggta gtcatggaaa acctcaaata ccagtagatt accagaaact tctatcgatt
      181 gaagacatta ccttttgccg caataggtgg ggtaacattg gtgaaaacgc attacgccgg
      241 gtggcagttg gaaaaaaatt atcttttttc ggctctgatc gtggtggaga aaatgcggca
      301 ataatctaaa tagctgcgct taataccgct acacttttgc cagcccatgt ttgcctccgg
      361 ggaatgggct gacggtttcc ataaaatggc gaactttttt caacagttgc cacattgagc
      421 ggcactgatg attacgcgtt attgtgtcgt gaagtgcctg ccatagccgt tcaacatgat
      481 tcacccatgg cgagtaaacc ggctgataaa tgcccctgaa cttcgggttc tccttcagcc
      541 agctctgtgt ttcccggctt ttgtggataa tgtagttgtc cacgatcagc gtgatggttt
      601 tcgcacgacg gtatgtcgct ttaagccgct tcagcaggct gatgaacagc gccgaacttt
      661 tgctgttgcc gcccacatag ctgactttac ctgtcccgct gtgcagcgct ccggccagat
      721 aatatttttc attctgtccc ggcgtgacca cccgtttttg ctgtccgcgc agttgccagt
      781 ccgcaccgat tttgggatga agatggatat ccacttcatc ttcataaaag accggatgct
      841 ctgcgctgca ttcgtccagt gctttatgga ttgctgccat cttttcatct ttatgcgggt
      901 cacggatacg cagagttggc gcagcccttc gccacacaat ccccacagac ggcaaccagc
      961 ggcgaacggt tccggcattt aactggcaac cggttatctc attgattttt attgccagca
     1021 gttctgtact ccagcgtgaa cgctggtagc caaagtcgcc gggagaatgt tttaccagct
     1081 cacgtaacag tgtgcagata tgctcaaacg gccagcgacg ggcacgcccg gcaggtaatg
     1141 atttcagtcc ctcaacaccc gactgcgtga accagttaat ccagcgtcca acagaggagc
     1201 gggcgcagca gagcgttctg gcaacgtcgc tgacacggtc gc
//
LOCUS       DAJTYH010000280         1236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTYH010000280 DAJTYH010000000
VERSION     DAJTYH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..307
                     /gene="curA"
                     /locus_tag="OO609_004200"
     CDS             <1..307
                     /gene="curA"
                     /locus_tag="OO609_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6635361.1"
                     /translation="PVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQDY
                     GHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            505..1170
                     /gene="mcbR"
                     /locus_tag="OO609_004201"
     CDS             505..1170
                     /gene="mcbR"
                     /locus_tag="OO609_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6635362.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          378 a          257 c          291 g          310 t
ORIGIN      
        1 tcccgtttgt ggcttagtga gcagctataa cgctacagag ctaccacccg gtccggatcg
       61 tttacccctg ttgatggcaa cggtgctgaa aaaacgtatt cgcttgcaag ggtttattat
      121 cgctcaggat tatggtcacc gcatccatga gtttcagaag gagatggggc aatgggtgaa
      181 agaggataaa atccactacc gcgaagatat tactgatggt ttagagaatg cgccacagac
      241 gtttatcggc ctgctgaagg gtaaaaactt cggcaaagtg gtgatccgcg tcgcgggtga
      301 tgattaaagt ttcaacggcg gcgtaagccg ccataaaaat ccaggcaaaa agcttatttt
      361 cagctttaat taactaacca cgtcattgtt ctgctgctaa taacaacaaa ggtgggtggt
      421 ttatgattat tcactttaat acactaggtg aactccttct gccatgcagg cagggttgga
      481 cagaaaacac gcgaaggaat taaaatgcct ggaacgggaa aaatgaaaca tgtcagtttg
      541 actctgcagg ttgagaacga cctgaaacat cagcttagta ttggcgcatt aaaacctggc
      601 gcacgcctga ttactaaaaa tctggcggag caattaggta tgagtattac acctgtgcgt
      661 gaagcattat tacgtctggt ttcggtgaat gcgctttctg tcgcacctgc acaagcattt
      721 acagtaccgg aagtgaggca aagtcagtta gacgaaatca atcggatccg ctacgaactg
      781 gaattaatgg cagttgccct ggctgttgaa aacctctcct cgcaagacat agcagaactt
      841 caggaattgc tcgggaaatt acagcaggcg caagaaaagg gcgacatgga acaaatcatt
      901 aatgtgaaca ggctatttcg cttagcgatt tatcatcgct caaatatgcc catcctgtgt
      961 gagatgattg agcaactgtg gatcaggatg ggacctgctt tacattatct ttatgaagcg
     1021 attaacccag cggaattacg ggagcgcata gaaaactatc atctattact cgctgcgtta
     1081 aaagcaaaag acaaagaggg atgcaaacat tgtcttgctg aaattatgca acaaaatatt
     1141 gctattttat accagcaata caatcgttaa attaaatgcc cggcaaatac cgggcatcaa
     1201 gtaattactc aaatcgccac gcaatattca tgccga
//
LOCUS       DAJTYH010000281         1211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTYH010000281 DAJTYH010000000
VERSION     DAJTYH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..799)
                     /gene="ipaH4.5"
                     /locus_tag="OO609_004202"
     CDS             complement(<1..799)
                     /gene="ipaH4.5"
                     /locus_tag="OO609_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6635363.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSL"
BASE COUNT          345 a          218 c          263 g          385 t
ORIGIN      
        1 gcagggattg cagaacgtga gtcgacaatg gattccctga aatgttaaca ttcgtacaaa
       61 tgggcaatgt gattatattt tcgggtaatg aagttagtag gttgttggaa acatccagtg
      121 ccaatagttc ttgcggtaat ctggataatc tgcgtagttg attttcacct accttcaaat
      181 attttaagct ctgaggaaat tctggtagtt cctgtagtct gttgccggaa acaaagagtt
      241 cttgtagtct atgaggcaat tcaggcaaga cagtaaggcg attaccttca atacgtaata
      301 ttgttagcgt tgatggaagc tctggtaacg tctccagact attaaaacgt gccgagagtg
      361 attgtaaata tggtggtaat gatggcagac atgacaaatt tcgattgaat gaaatattaa
      421 gcttaatcag ttgagatgga agtgtcggga gtatattcaa gttgttacca tttacatgaa
      481 gttctgtcaa aagcggagaa ttttcaggta gggagattaa ctcattgttg gaaatattaa
      541 gttcacgtat atgcaaaggg agtggtggta aagaacgtag tttcagtaag cttaaattga
      601 gaacaggctc ccgggtatcc agacatattt tcaataatcg aaccgcctgg atgcgatttt
      661 ctgttgttgt tccttcccta gcccagtcat cccagatgcg gtggtaatct ctggtacact
      721 gttcttctac cgataaacga gatatacatg ataagccaca aagggaacga aaaaaagaat
      781 gattgttgat cggtttcatt tattaacccc tcactattca tatcttggtc tattaatgca
      841 aataaaacga gaaagtcttt aaaaaaacaa tcctagagct ttatcggcgt tttcattccg
      901 gctggaggaa tatgttcctg tctttccagt aaccgtcgat tgttatatca gtctacccat
      961 gttaatgtga ccagttctac tttcatccag tttggattga tctttatccc ctatattttc
     1021 aaacaatttt tatcatttaa ctcattactg tctcgatatt cccatagcaa acgatctggt
     1081 tggttatccc ctcaaaaaag tcagttgcac gccgtaaatc cctgccggta ttgagcgagg
     1141 atttacgggt tgttgttaag gtccgtaatc gggtcacagt ttttccggag tcaatccggt
     1201 ctgcggttta t
//
LOCUS       DAJTYH010000282         1185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTYH010000282 DAJTYH010000000
VERSION     DAJTYH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..950)
                     /locus_tag="OO609_004203"
     CDS             complement(<1..950)
                     /locus_tag="OO609_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6635364.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDESMERFSWHVADKGFRMVRYYVF"
     gene            complement(950..>1185)
                     /locus_tag="OO609_004204"
     CDS             complement(950..>1185)
                     /locus_tag="OO609_004204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635365.1"
                     /translation="FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTE
                     REFATILAGISAGGMYCPECGTVHWPDGVAPPF"
BASE COUNT          277 a          356 c          291 g          261 t
ORIGIN      
        1 aaaaaaacgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
       61 tccatggact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      121 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
      181 ggctttttca ggtaagagcc aaagtacacc gccacatggg tggcattatc cattacccgg
      241 gatacgttga cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
      301 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtcagggta tttccgtgac
      361 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
      421 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
      481 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atatccccgg ctccacatct
      541 gcctgatggc agatttccag tatcacatcc gctgcaatgc ggctcatctc tgccagtaac
      601 caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc
      661 cacgggcagt aggggaccag gctcagcaga tactgtatcc actgtgcgcc agccttcacc
      721 ccgcagtgcg ggcaggagcg gcttttacac cggaagcaga ccttttttgt atggcaacag
      781 tccggtgatg aacaacacca ctgcgtgtag cccatcagtg tggtcccgca ggccatgatt
      841 tttgtcactg attcaattac caccggacgg actgcccctt ccggttgttt ctccagccag
      901 ttaagccagc ggtttccctg ctgaaaaata tcggcaaaac gggggagcat cagaagggcg
      961 gggcgactcc gtccggccag tgaaccgtgc cacactccgg gcagtacata ccgccggcgc
     1021 tgataccggc aagaatggtc gcaaattccc gctccgtgca gcgggcgatt tccggatacc
     1081 cttcgtcatc aacacgtaca aaccagaaga ccagcttttt gtttcccgca tccacaaaga
     1141 acggaatatt caggtctgcg cagcattcaa cggcatcgtc aaaac
//
LOCUS       DAJTYH010000283         1174 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTYH010000283 DAJTYH010000000
VERSION     DAJTYH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            613..>1174
                     /locus_tag="OO609_004205"
     CDS             613..>1174
                     /locus_tag="OO609_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6635366.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPR"
BASE COUNT          328 a          251 c          271 g          324 t
ORIGIN      
        1 gtgttaatat cctggataac tgtgcttgaa ggatttctgt taataatctg ctcctgcaac
       61 tcacctgatg agtattagtg gtaatacata atcttactcg taaaagagta taccttatgt
      121 cattaaacag tcttccggcg tgttgttcaa tcctgtacct ctgttactta tctggtacgt
      181 tgatatgtgt aatgcaacgt taattgcaat atctggttat ggctttatac cttcagcatt
      241 taaaatattt tattaccgga gaaacaggga agtggaaaga ctttcgggat aatgtcaggc
      301 cattctgacg ttaatcacag taccaaataa cgagagggta atggaagtta aaacctgcgt
      361 aagttcctct gttgttcact ggaaaaagtg attgcccaca cgcgttataa aggcctttcc
      421 gctgcagcct ggttaacaga gcttcaggcg ttatatgcgc tgtatggctg ttacgtatgc
      481 tgttctttgc ctggttctga tacccgtgga agtcgttacc ggaggaaaca cgttcaccct
      541 actacctgag aacatcttct tcaaaaatta cggcaacgct tcttcctgtg ttacagaaca
      601 cgaggaagaa acatgcaaac ttataacacc aatcccgctt acgaaatggc tccccagctc
      661 ctggagcact ttaaccagca tctggattca ttattcgggg tctattcgaa actgttaccg
      721 ttcagaatgg attttgctta ccgaaaaaac accctcagct acagatgtgc ctgcaggtac
      781 gccatgtgcg cggagatgct gcaactgatt aacgaggtgg gagagaaact ggtcgggtat
      841 gcctgggtga tggaatatac agaacgtaaa gggcttcaca ttcattttgt gggctatctg
      901 aacggccaga gccacaggag ttcgtatctg gtatccagat tgatgggaga tatctggagg
      961 cgggttaccg agggtaatgg ttactaccac tggtgccggt ttaataaaaa ttatccggta
     1021 aacattaacc acgtcattca ctattcagat cacaaagcgg tgaatgattt acgctatgcc
     1081 atcagctacc tggccaaacg ggaacagaaa gaatgtggca tcatcctgaa atgcagcggg
     1141 cttcctgaaa aaagtaaccg gggtcgacca cgac
//
LOCUS       DAJTYH010000284         1137 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTYH010000284 DAJTYH010000000
VERSION     DAJTYH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..213)
                     /locus_tag="OO609_004206"
     CDS             complement(<1..213)
                     /locus_tag="OO609_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6635367.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGI"
BASE COUNT          330 a          270 c          239 g          298 t
ORIGIN      
        1 aataccggtc actcgagaac ccggccagtc ttgcgactgc cctgcacatc ccaccataat
       61 ttgaatggag ctgtgcatta cgcataaaaa aaccgcttca gcgcggctat gcgctcgagt
      121 aacgttcggg tttccacgcc cggcccccgt tttatgaggt gcagatgcac tataattcca
      181 cccgttctgg ttttcaatag ctacattcaa cattttctct tacctttcat caccgaagtg
      241 aactttgttg atgcggtgcc tggtgcctcc aggtgacgtt aaccagttaa caattaacgc
      301 cggatacagg gaaacccata accacccgat acgtgggaac taatcgcttt ttaactgttc
      361 cgcgtgcgct cagccgcatt caccgcatca caaaattcac tttaaaaagg gcggacatca
      421 gccagcaatg aaactgatgc cgccaaaggt accaatcaac atggagtgtt gtggcgtggt
      481 tgtcacttaa gcgtatggtc aacctgacaa cccggtgtcc tcaacgggga aggaataact
      541 ccgccatact taccgccgcg tcatttcgcg ttgtgtgcct gcttttaact acgtcaggcg
      601 aggtggttcc tgttattccc caacaacaag aaatttgtat aatctgaata ccccaacaat
      661 aagaagagtg agcaagtgat gtattagcga tgtcagctct caaagagact gcatcaatgt
      721 taaaattatt caatgaagca aaaactcagg ctgaggttga tcgcgctgtg tctgaaataa
      781 actctaagtt gtcaagcatt cagcttgaat atttgtcatc tcattgatga gctgacaatc
      841 tctaaaagca atgaggctgc gctcaaagca aaactcacgg agatagaaaa gtttaagttc
      901 caaagtgaag actgcgaacc tcgctaattg attactggaa cgattgtgta tgccagacag
      961 tgcaatacta gctctggcaa gattaccatg tatttttgtc cagaatgttt tgcgaagaga
     1021 gcaatatcta tattgcaacc tttccctatc gaggaaggcg ataaatatac gaaaactcaa
     1081 tgcccctcct gtaaaaatga gtatctcatg gactacaatt tcaataatga tttttga
//
LOCUS       DAJTYH010000285         1082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTYH010000285 DAJTYH010000000
VERSION     DAJTYH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(21..824)
                     /gene="dkgB"
                     /locus_tag="OO609_004207"
     CDS             complement(21..824)
                     /gene="dkgB"
                     /locus_tag="OO609_004207"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6635368.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(987..1063)
                     /locus_tag="OO609_004208"
     tRNA            complement(987..1063)
                     /locus_tag="OO609_004208"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1027..1029),aa:Asp,seq:gtc)
BASE COUNT          283 a          253 c          247 g          299 t
ORIGIN      
        1 ggaggagctg tcagagaggc ttaatcccat tcaggagcca gaccttccgg gctaaccagg
       61 cggtcgttgc aatccagtgc ggcgatcgct tttttatctt cggcatcaag ctgtaaattt
      121 tgtgccttaa gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa
      181 ccttccccca tagcccacgc cagaatcact tgtgccggaa tcgcattgtg tttagctgca
      241 atacgagcaa taacctcatc tttcagtgcc ttaccataag ccagcgtcat ataggaagtg
      301 atatgaatac cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag
      361 agttcaatct ggttggtagc gatgttttca gcgccaacag cagcaatcac tttttccatc
      421 aatgggatcg tgaagttgga aataccgatc tcacgcgtca gcccttgttt tttggcttcc
      481 agcagcgcct gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt
      541 agcgtcagat caacataatc ggtacgcaat ttttgcaggc tctctttcag actcgggatc
      601 aatttgtctt tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt
      661 ggcacgccac tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgcg
      721 gtatcaattg cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg
      781 tctttcagac ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa
      841 tgctattttc gttaactgag gtgattatgg gcttcagact gttgcgggaa aagagcgtaa
      901 aacgcagagg atttttgcga ttctagcaat aatagatatc cagacgcaaa aaaacatcga
      961 gcgttaactc gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc
     1021 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa
     1081 cc
//
LOCUS       DAJTYH010000286         1079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTYH010000286 DAJTYH010000000
VERSION     DAJTYH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..1009
                     /locus_tag="OO609_004209"
                     /pseudo
     CDS             1..1009
                     /locus_tag="OO609_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          305 a          265 c          252 g          257 t
ORIGIN      
        1 caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg aagagtttta
       61 ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac ccgaatggca
      121 gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga taaccgctcc
      181 tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac gtgagtttgc
      241 cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg cggatgagtc
      301 ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg acgataaacc
      361 atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt acgactggaa
      421 agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc cactgtataa
      481 cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac cttttggccc
      541 ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct gggctgctat
      601 gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg acaatcattt
      661 ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat atgcattatt
      721 gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa tcgccattta
      781 tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag gggacattgg
      841 cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata acgattactg
      901 gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt ataacgaacg
      961 gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac gcaggattta
     1021 acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa tgaaacccg
//
LOCUS       DAJTYH010000287         1057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTYH010000287 DAJTYH010000000
VERSION     DAJTYH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..861)
                     /locus_tag="OO609_004210"
     CDS             complement(<1..861)
                     /locus_tag="OO609_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6635369.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLTYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTT"
BASE COUNT          309 a          273 c          211 g          264 t
ORIGIN      
        1 cgtcgtgcga atatagccaa tcagctgctc ttcgattaac tgcttgcagt cccagatact
       61 gttaaacact tcaccgaccg caatcggcgt cacggtatgc tggcgaatca gacgaaaaca
      121 ttcctggttc tcggcaggcg tcggatcttc catccagaac aggcggtaat cctcaatgct
      181 cttcccaaag cgcgccgctt cgatgggggt caggcgatgg tgcatgtcat gcaacagatg
      241 ttcgttaaaa ccgaacttat tacgcaccgc ttcgaataac ttcggcgtaa aatcgagata
      301 cttttctgaa gaccacaact gctcttctgg ccacaggcct ttggttgccg gttcatacgt
      361 cagtcctttg cccttcgcca ggccataagt ggtttgcata cccggtacac cgcactgcac
      421 acgaatcgcc ttaaagccca tctgctggtg ctttgcatag tcttcaagga cttcatcaat
      481 cgtacgcccg gtggtgtggc aataaaccat cacgccttca cgagaagcac caccaagcag
      541 ttgatacaat ggcatatttg ccgctttagc tttgatatcc cacaacgcca tatcaatggc
      601 agaaatggct gacatcgtga cggggccacg tcgccagtaa gcacctttat aaaagaattg
      661 ccagatatct tcgatacggc tggcatcgcg gccaattagt tgtggacaaa gatgatcttt
      721 cagataagaa gcgaccgaca attcgcgacc atttaacgtg gcatctccta atccacaaag
      781 tccgctttca gtggttattt taagcgtaac gaaattacgc cctgggcaag taacaaaaac
      841 atctgcagca ataattttca ttattgcaat ctcctgtgtt gtaaatagac gtttcaaacc
      901 taattttacg cctactacca tacaagagag aaaaagcgat tattcaaccg tgatcacatt
      961 aaaaacaaaa atttctaccg aggagaaaaa ataccctcaa aaaccacctg aataattaac
     1021 cattattgtt aaaacaacac gttgaataat cacataa
//
LOCUS       DAJTYH010000288          949 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTYH010000288 DAJTYH010000000
VERSION     DAJTYH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(203..868)
                     /locus_tag="OO609_004211"
     CDS             complement(203..868)
                     /locus_tag="OO609_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6635370.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          246 a          232 c          215 g          256 t
ORIGIN      
        1 atgtaagagg ctaacctaca tgtgcaaagc atgaaattgg cctcagatta atgttaagcg
       61 tcttgccgga cgcgtaatgt taactggggc ttttctctat ctgccttttg gtgttcatgc
      121 ctgaagcaga tagcctcaag cacccgcagt cattctactt acctcgccaa tatgaaatca
      181 atcagaaagg tgccccataa aatcactcct tctcttcttt accgtagtgg agttgaccaa
      241 ttttgataag agggcgtccc tgagatttgc ggtgtagatt ggtatcgcgc agagaataca
      301 cacagccaca atattcctgc tgatagaatt tttcgcgctt gctgatttca atcatacggg
      361 acgagccgcc ctgcttgcgc cagttataat cccagtacac catgcccgga taatgcgcga
      421 cggctcgccg cccacagtcg ttaacctgct gcatattttt ccagcgtgaa atgcccagtg
      481 aactgctgat cacactgaaa ccattttcag cggcgtacag cgctgtccgc tcaaaacgca
      541 tgtcaaaaca catggtgcaa cggatccctc gttcgggctc ccattccatt cctttggctc
      601 gttcaaacca gttgtctgtg tcgtaatcag catcaataaa cggcacgccg tgttgttcag
      661 caaagcgaat attctcatcc ttacgaatta aatactcttt ctgaggatga atgttcgggt
      721 tgtagaaaaa gatggtgtag tcgatttccg aggcctgaag cgcctccatc acctcaccgg
      781 aacatggagc acagcaagag tgcagtagta gtttgtttgc cccgtttggg agctccaatt
      841 tgggccgttt gaaatcagca actgtcataa atatgtttat tgaggtcata aaaatatcaa
      901 agagtgtagc attagaacgg ggctatcgga aacagatgtg tgactgctc
//
LOCUS       DAJTYH010000289          924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTYH010000289 DAJTYH010000000
VERSION     DAJTYH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            103..>924
                     /locus_tag="OO609_004212"
     CDS             103..>924
                     /locus_tag="OO609_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR6635371.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRP"
BASE COUNT          246 a          218 c          232 g          228 t
ORIGIN      
        1 acgaacgtca aatatagttg tttactgtgc gctgttgtcc atgtgatatt gtgtaccaga
       61 tgtgtgccaa actattattt ctgagcgtac caaatattat ttatggctat aagcgacaca
      121 aaactgcgta ctatttatgg taaaccatat tcgggcccac aagaagtggc tgatgccgat
      181 ggcctcagcg tacgaatttc accgaagggg gtcatccagt tccagtaccg ctatcgctgg
      241 catggcaagc ctaatcgact tgggcttggt cgatacccat ccctgtcttt gaaggatgcc
      301 agacagatca ctgctgactt gcgaaagctc tatttctcag gaacggatcc acgcacctat
      361 tttgaagaga aggtggagaa ctccatgacg gtcgcccagt gtctcgacta ctggttcgac
      421 aactacgtct ctacaactct cagagaaaag acccaggcac tttatcgatc aacggttatg
      481 aagcgcatgc atgacgcctt ccctaatcgt ccggcatctt ctatcacggt taagcaatgg
      541 gttgacctgc ttaccgaaga agaaaaagat aatccacgcc gagcaaggca ggtgctaagt
      601 caactaagat cagcaattag ttggtgcatg cggcgtcagt tgatagatag ttgcgcaatt
      661 atgagcatcc agccaaggga cttcggctcc cgcgctgagg taggggatcg ggtactgtcg
      721 tatcacgaac tggctaagat ttggcttgct attgaaagaa gccgtgcgtc tacgtcaaat
      781 aagctccttc atcagatgct tatgctgtgg ggggcgaggc tctcagagct taggctggca
      841 acaaagacag aatttgacct gctggacaac gtatggaccg taccgaaaga gcatagcaag
      901 atgggtaatg ttatccgccg tcca
//
LOCUS       DAJTYH010000290          922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTYH010000290 DAJTYH010000000
VERSION     DAJTYH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..254)
                     /locus_tag="OO609_004213"
     CDS             complement(<1..254)
                     /locus_tag="OO609_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCR6635372.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRG"
     gene            complement(266..784)
                     /gene="cusB"
                     /locus_tag="OO609_004214"
                     /pseudo
     CDS             complement(266..784)
                     /gene="cusB"
                     /locus_tag="OO609_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT          215 a          261 c          244 g          202 t
ORIGIN      
        1 aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag cggataagtc
       61 acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat aatcacctgc
      121 acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca ggtgccccag
      181 atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga gcgacgaata
      241 atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca gagcgcatcc
      301 gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg ccggaaacca
      361 ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat gcctggaaaa
      421 cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc cgctgttcgt
      481 tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg gcggtgttga
      541 gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg acttccagac
      601 gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat ttgcggatgg
      661 tgagtgtttt atccggtcgc gccggaacgg tgagggtaaa ctgcgaggca tctttcacca
      721 gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata ccctgaattc
      781 gcgattacgc gcaatccccc tgagaaccgc catgaacaag ggtttcatga tttgtgattt
      841 tagtttggta cgcaatttgg tacacaacac tattttcact tcagcgggta gtcatcaaac
      901 tcaccataac gagcatcgtt ga
//
LOCUS       DAJTYH010000291          921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTYH010000291 DAJTYH010000000
VERSION     DAJTYH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(174..764)
                     /gene="uidR"
                     /locus_tag="OO609_004215"
     CDS             complement(174..764)
                     /gene="uidR"
                     /locus_tag="OO609_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6635373.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
BASE COUNT          235 a          218 c          225 g          243 t
ORIGIN      
        1 taagcaatcg aacgttcgtt cgagagtgtt gggaacgata aactctcatg acatatggct
       61 acggtgaata ttttgggggg gaattctccg aacacgggga ggataatttt tgcacagaga
      121 aattacgata tgaaacgcgg gcacagttat caccagcccg cgaaatgaga ggatcaggat
      181 gcggttaata taccgccaat catcgcgcgt aatccctgag caaacgacgc ttcacgcgcc
      241 aggtcttccg cttcgatatc ggccagtaca ccgtaggtca gatccagcag taaacgtgaa
      301 gtcattgccg tgttgatgtc tgggcttatc tcgccttttt gctgggcatc acgcatccgc
      361 tgggcaacaa attcagtgat cgtcatatgc ttatttttaa gcatggcggc gacctgtggg
      421 ttacgcatcc cttccgccat aatttcgacc accagcgccc gttgcccaaa ggcttcatgg
      481 gtgagagaga caatagactc gaccatatag tcaacaaaat gaatcccttc aatcggttcc
      541 cggaaacgag ccagcgccct ctcctggtcc tgtaagataa tcgcctgaat caaggcttct
      601 ttggagatga aatggtgata gagcgtccca ggactaatgg cgcaagattt acagatggct
      661 ttcatcgagg cactgtgaaa tccattttct gaaaaaatct ctctggcagc attgaggatc
      721 cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca tcattaaacc attatagtta
      781 gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct atcattgctg ttgaatagtg
      841 cgaaggtaca ctctattcgt atacttttca cccagcaatt ggttaaccaa tagccgtgat
      901 ttggctgcca gttgtttagc a
//
LOCUS       DAJTYH010000292          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTYH010000292 DAJTYH010000000
VERSION     DAJTYH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            78..368
                     /locus_tag="OO609_004216"
     CDS             78..368
                     /locus_tag="OO609_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6635374.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            365..>902
                     /locus_tag="OO609_004217"
     CDS             365..>902
                     /locus_tag="OO609_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6635375.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGD"
BASE COUNT          248 a          187 c          263 g          204 t
ORIGIN      
        1 aactggatcc tgttccgtaa attttcctct ccggaggaag tcgaaaatgt ggccgcgcag
       61 ttactggagt ttgcgtaatg gtggatttac gtaaagcggc gcgggggcag atgtgcaccg
      121 tcagaattcc tggctactgc aatcacaatc ccgaaacttc tgtgctggcg cattacaggc
      181 tggcggggac gtgcggaaca gcgacaaaac cacacgatat gcaggcagcg attgcctgta
      241 gctcatgcca cgatttaatc gacgggcggg taaaaaccag cgattacacc aaagaagaat
      301 tacgcctgat gcatgcagaa ggtgtttttc gcacacaaga aatctggaga aaggaaggtt
      361 atttatgatt tacccaacaa atacaggcaa aagcggggaa caccttcgtc tcaccacgct
      421 ggaaagtgtc tggattcagg gaaaactgcg catgtggggg cgctggtcgt atattggcgg
      481 cggtaagacg ggaaatatgt ttaaccagtt gctggcatcc aaaaaattga cgaaaacagc
      541 cgtcaatgaa gccctgcgca ggatgaaaaa agcgggaata gagaaacctg agctggaagc
      601 gtttttgcga gagatgatca atggcaagca aaagacctgg ctggcgcatt gtactgatgc
      661 agaggcgtta tgtattgata gagtcataag tgaggtgctg gcagagcatc ctggattgat
      721 tagtgtcctc cggcaacggt atgaagggcg ggggatgact aagcgtaaaa tggctgaatt
      781 gctgaatgac gcacaccctg agtggtgttt ccggacgtgc tgcagtcggg tagatgtatg
      841 gctaaatctt gctgaatata tgctctatct accgatgcgt gatgcattct cttccgggga
      901 tc
//
LOCUS       DAJTYH010000293          894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTYH010000293 DAJTYH010000000
VERSION     DAJTYH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..146)
                     /locus_tag="OO609_004218"
     CDS             complement(<1..146)
                     /locus_tag="OO609_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6635376.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFQNTV
                     TNLN"
     gene            complement(214..>894)
                     /locus_tag="OO609_004219"
     CDS             complement(214..>894)
                     /locus_tag="OO609_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR6635377.1"
                     /translation="GMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTEL
                     NVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIV
                     LPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMA
                     HLLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGG
                     IQYAPPVR"
BASE COUNT          226 a          231 c          210 g          227 t
ORIGIN      
        1 ttattgagat tcgttacagt gttttgaaat aaccaaccaa caatgccgac aacagcaaca
       61 acggcaagga tctggaataa ccaggcacga accgtagggt tggcaaagga gagtgagcct
      121 ttaacggttg agcggcgatg aggcatagaa acctcggaaa gcataacgct gaagagagcg
      181 ccgccgtagc gacgctcgtt acctcacacc gacttaacgc acgggcggcg cgtactgaat
      241 accgccgtta ttccagagat tattttgccc acgtttaatt ttcagcgggc tttctgaacc
      301 tacgttacgc tcaaaaattt ccgagtagtt actcacctgt ttgatgatgt tataggccca
      361 tttattatcc aacttcagat ccttgccgta atcgccttct ttacccaaca gatgtgccat
      421 atcaggcgtt gctggattag ccgctttttc atcgacgttc tgggaattga tgcccatctc
      481 ttcagcattt agcatggcga aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc
      541 acgacgaact accggcccaa gcggttcttt agagataacc tccggtaaga caatccattc
      601 agccgggttg cttaatttga tgcgcaaggc atacagttgc gactgatccg aagccagcgt
      661 atcgcagcga ccagattcca gtgccttcgc tgattcgtca gagcgatcga aagtcactgg
      721 tgtgtacttc atattgtttg ccttgaaata gtcggcgacg ttgagttcgg tatcagtacc
      781 cgcctgaata cagacggtag cgccatccag ttctttcgcg ctttttagcc ccgctttatc
      841 gtgcgtcagg aagccaatgc cgtcgtaata agtgacgccg gtaaatgcca ttcc
//
LOCUS       DAJTYH010000294          884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTYH010000294 DAJTYH010000000
VERSION     DAJTYH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..289
                     /locus_tag="OO609_004220"
     CDS             <1..289
                     /locus_tag="OO609_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635378.1"
                     /translation="LPAVIEKSVEPMKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGN
                     LAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLISTTPVAENVK"
     gene            528..587
                     /locus_tag="OO609_004221"
     CDS             528..587
                     /locus_tag="OO609_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6635379.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
BASE COUNT          233 a          210 c          241 g          200 t
ORIGIN      
        1 gctacctgcg gtaatagaaa aatccgttga gccgatgaaa tctattgacg gtatcaagat
       61 tattcaggtc gatggtctga atagtggcag cgctgcgggt gatgcaaaca cgggtaatgt
      121 ggggggcgga aacctagcgg aacaagcatt atcagccgct ctctcttacc gcacacaggc
      181 tccgctgatt gactccttgc tcaatgaaat tggcgtttca ggcggttcac tggcggcatt
      241 gacttcaccc ttaatctcaa caactcccgt cgccgaaaac gtaaaataaa ttttcagcta
      301 tgaacgtccc tggcacaaaa agccggggat tttttatatc tgcgttccgc taaaaactgc
      361 aaatgatcag gtcgttgcaa cgttttgcgt gaccgctcgg ggaaggcaaa actgcctctg
      421 ggaaagcgtt gcgcgaggtc cggcgctcat caacaatcgg ggagtcagca aagggctgaa
      481 acgggaaagc ccctcccgag gaaggggcct tacagaagga aagggttatg atgaagctcg
      541 tcatcatact gattgtgttg ttactcgtaa gtttcgcagc ttattaacag ccaatcagag
      601 gggggagaaa tcctccctca cccttgttcc tttactttag gttgaaaaaa aatcagcgtc
      661 aagaaggttt cattaacaca tatccctggc ccaaaaagcc ggggattttt tatatctgcg
      721 ttccgctaaa aggtgcaaat gctcaggacg ttgcagcgtt ttgcgtgacc gctcggggaa
      781 ggcaaaactg cttcagggaa agcgttgcgc ggggtccggc gctcatcaac aatcggggag
      841 tcagcaaggg gctgaaacgg gaaagcccct cccgaggaag gggc
//
LOCUS       DAJTYH010000295          884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTYH010000295 DAJTYH010000000
VERSION     DAJTYH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(95..814)
                     /gene="yehT"
                     /locus_tag="OO609_004222"
     CDS             complement(95..814)
                     /gene="yehT"
                     /locus_tag="OO609_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6635380.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
BASE COUNT          223 a          240 c          192 g          229 t
ORIGIN      
        1 ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta cttatcatga ggccttcagg
       61 tgttgatgag gcaaaaagcc attttagcag tcttttacag gccaatcgcc tcttttaagc
      121 ttttcagata acggcggctg accggcacgg ttaagccatt acgcaaaatc aactcggcct
      181 ggccgttatc ttccagacga atctcctgta aatgcgcgag gttaaccaga tactgacgat
      241 ggcagcgcag tagtggtgta cgactttcca aggtacgtaa tgtcaattcg gtaaagccct
      301 ctttcccttc gtggctggta acgtagacac cgctcatccg actgctgaca aatgccacat
      361 ctttcatttg cagcaaataa atccgactat gccccgtaca agggataaat ttcagcgcct
      421 gttgattttc cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc aatcgcgcca
      481 gcgttttctc cagtcgcgct tcatcaattg gcttcagcag ataatcaaag gcatgttctt
      541 caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt
      601 ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa
      661 acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg tttgaacact
      721 ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat acgcaggttc tcccgtgcta
      781 acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagtcgtaa
      841 cgttattcgg gtgtaactat caggctcaca ggcgacgctt attc
//
LOCUS       DAJTYH010000296          876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTYH010000296 DAJTYH010000000
VERSION     DAJTYH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>876
                     /gene="btsS"
                     /locus_tag="OO609_004223"
     CDS             <1..>876
                     /gene="btsS"
                     /locus_tag="OO609_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6635381.1"
                     /translation="MQVTVRLPHKFLCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAV
                     MGGLLGGPVVGGLVGLTGGLHRYSMGGMTALSCMISTIVEGLLGGLVHSILIRRGRTD
                     KVFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRLVSNIAAPMMVTNTVGAALFMRI
                     LLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNEVNSMKVAQVLYQELDIGAVAIT
                     DREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEVVYADGNEVPYRCSLHPQCKLGS
                     TLVIPLRGENQRVMGT"
BASE COUNT          187 a          217 c          245 g          227 t
ORIGIN      
        1 aatgcaggtc acggttcgtc tgccgcataa atttctctgc tacatcgtct tttccatctt
       61 ctgcatcatg ggcacctggt ttgggttgca cattgacgat tctattgcca atacccgtgc
      121 gataggcgcg gtaatgggcg gcttactcgg cggtccggtc gtcggtgggc tggttggtct
      181 gaccggcggc ttacatcgat attcgatggg gggcatgacc gcgctaagtt gcatgatctc
      241 aaccatcgtt gaaggattgc tcggcggcct ggtacacagc atcctgatcc gtcgcgggcg
      301 cactgataaa gtctttaacc ccattaccgc cggtgccgtc acgttcgtcg ctgaaatggt
      361 gcaaatgctg atcatccttg cgatcgcccg accttatgaa gatgcggtgc gtctggtgag
      421 taatattgct gcgccaatga tggtcaccaa taccgtcggc gcggcgctgt ttatgcgtat
      481 attgctcgat aaacgcgcga tgtttgaaaa atacacttcg gctttttctg ccactgcgct
      541 gaaagtggca gcctcgacgg aaggcatttt gcgacagggg tttaacgaag tgaacagcat
      601 gaaagtggct caggtactgt atcaggagct ggatattggt gcagtcgcga ttaccgatcg
      661 agagaaattg ctggccttta ccggaattgg tgacgaccac catttacccg gcaaaccgat
      721 ttcttcgact tacaccttaa aagcgattga aaccggtgaa gtggtctacg ctgatggcaa
      781 cgaagtacct tatcgttgct ctttgcatcc gcaatgcaaa ctggggtcga cgctggtaat
      841 tccgttgcgt ggcgaaaatc agcgcgtgat gggcac
//
LOCUS       DAJTYH010000297          866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTYH010000297 DAJTYH010000000
VERSION     DAJTYH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..324
                     /gene="pgrR"
                     /locus_tag="OO609_004224"
     CDS             <1..324
                     /gene="pgrR"
                     /locus_tag="OO609_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6635382.1"
                     /translation="INLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAI
                     DGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLK
                     YKGAV"
     gene            661..>866
                     /locus_tag="OO609_004225"
     CDS             661..>866
                     /locus_tag="OO609_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6635383.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGL"
BASE COUNT          216 a          167 c          196 g          287 t
ORIGIN      
        1 attaatttgt atcttcccac atcgggtaca gcaaatcgct ggagattaat acgcggtgga
       61 cgtgaagttc gtgttcgcat ggaaggtcag cttttactga atacgataga cctgatcatt
      121 gatgctgcaa ttgatgggca tggattggcg tatctacctt atgatcaggt tgagcgggct
      181 attaaagaaa aaaaactgat acgtgttttg gataaattca ctccagattt acccggttat
      241 cacctgtact atccacaccg tcgacatgct ggctcggcat tctcattatt tatagatagg
      301 ctgaagtata aaggtgctgt ttagcactac ttgctgatac attaatttaa ttcttctctt
      361 aacgtattct cagttccttt caacgttttg gttatttttt attcttcgta caatggcgac
      421 agatgctgat tatgataccg aaaactggtt tgaacgtgcg aagcccgaac gtagtgttcg
      481 gtgttctatg tgctttgcca cattttggcg tctattattt acactaaatc tgatttgata
      541 tattgatact taaaacattt gatgcttcct ttgtcacttt tttgatggaa gttgtttgca
      601 tttctttaag gcgaaacaaa taattacgca tcaattttaa tgtcggttag agggaaactt
      661 atgaagcact ctgtttcagt tacgtgttgt gcgctgttgg tcagcagcat ttctctttcg
      721 tatgctgcag aagttccgag cggcacagta ctggcagaga agcaggagct ggtgcgccac
      781 attaaagatg agcctgcgtc gctggatccc gctaaagccg tgggcctgcc agagattcag
      841 gtcattcgcg atctgtttga aggtct
//
LOCUS       DAJTYH010000298          841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTYH010000298 DAJTYH010000000
VERSION     DAJTYH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            59..556
                     /locus_tag="OO609_004226"
                     /pseudo
     CDS             59..556
                     /locus_tag="OO609_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            597..788
                     /locus_tag="OO609_004227"
                     /pseudo
     CDS             597..788
                     /locus_tag="OO609_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          216 a          210 c          244 g          171 t
ORIGIN      
        1 gcgccagccc cgtcccgccc gatagagcgc ggcatagcgg gccccggatt acttgcgtgc
       61 gacggcagaa gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg
      121 gggattacca gagtcagagt gtcttgcagt caggcagcag cgaagcaaag cgttactgac
      181 gtcgctgcat gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg
      241 cgaagcattc agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgacgg
      301 tctggcggag gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa
      361 gaaaaattac gtgttcttcg gtagcgatca cggcggcgag cgtggtgcac tgctgtacgg
      421 gctgatcggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
      481 gagcgtactg ccggaatggc cttccaaccg agttgacgac ctcctgccat ggaaggtggt
      541 gctaccatca ggataatcgt caatacggtg ttcggtgaac gtttacttgg agcatcatgc
      601 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
      661 ccttcccggc ggtgaaaatg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
      721 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagtct
      781 tactggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
      841 a
//
LOCUS       DAJTYH010000299          833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTYH010000299 DAJTYH010000000
VERSION     DAJTYH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..274)
                     /locus_tag="OO609_004228"
                     /pseudo
     CDS             complement(1..274)
                     /locus_tag="OO609_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(322..687)
                     /locus_tag="OO609_004229"
     CDS             complement(322..687)
                     /locus_tag="OO609_004229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635384.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCMEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
BASE COUNT          205 a          230 c          184 g          214 t
ORIGIN      
        1 tgtgatttgc ctcgccgttt ttctcactgt ctccgttatg gcgtacacca catcttccag
       61 taatcgccgc ttcaccggac tcaggacccc gtatcaccat ccggaacccc ttatccgcca
      121 catgccagga gaacctttcc ataaactcgt cgccactcat cacaaggttt tcttctcgtt
      181 ttgtccggtg gctgttgtaa cgcaggccaa tttcatcctg accggcataa tgttccaggc
      241 ggctcatctg caccggctgc ttctccagcc agttaagcca tcggtttccc tgctgaaaaa
      301 tatcggcaaa acggggaagc atcagaaagg cggaataacg ccatccggcc agtgaaccgt
      361 accgcattca gggcaataca tgccgtcggc gttgataccg gcaggaattg tcgcaaattc
      421 ccgctccatg cagcgggcaa tttccggata cccttcgtca tcaacacgta caaaccagaa
      481 gaccagtttt ttgtttccgg catccacaaa gaacggaata ttcaggtctg cgcaacactc
      541 aacggcatcg tcaaaattat caaagcgcag aacctcagct tcttcttcgt caaaaaaatc
      601 atcttcgtga agttttacga catagcggag gagtttgctt ctttgaggtg caggttttcg
      661 tttgcgtggt ttagctgaac gggccatata actacctgaa agacaatgat attgtctgtt
      721 tttataacgg taatcacagg ccatgacaag ccgcagccgt caggccgcct actcggggcg
      781 gtttagaacg cttatttagt accagatctt acttataata aaggggcttg gca
//
LOCUS       DAJTYH010000300          829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTYH010000300 DAJTYH010000000
VERSION     DAJTYH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>829
                     /locus_tag="OO609_004230"
     CDS             <1..>829
                     /locus_tag="OO609_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6635385.1"
                     /translation="NGERELDALVLPYKRDGELLQVKRISTERPDGKKVIMAEGDCEP
                     CLFGWQALDAGVRVVVLCEGEIDCMSYAQYGISALSVPFGGGKGAKQQWIEFEYHNLD
                     RFEEIFISMDVDDVGREAAREIVSRLGEHRCRLVTLPYKDINECLMNGVTEDEIWQYI
                     GTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWESLADKFQFREAELTLVNGVH
                     GHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFE
                     "
BASE COUNT          207 a          176 c          245 g          201 t
ORIGIN      
        1 aatggagaac gagaactgga tgctctggtg cttccgtaca aacgcgatgg tgagttgttg
       61 caggtcaagc gaatcagcac tgagcgcccg gacgggaaga aagtcattat ggcagaaggt
      121 gattgcgaac cttgcctgtt cggatggcag gctctggacg ctggcgtgag ggtggttgtg
      181 ctttgcgaag gcgaaattga ttgtatgagc tatgcgcaat acggcatctc ggcgttatcc
      241 gtgccgtttg gtggcgggaa aggcgctaag caacagtgga ttgagtttga gtatcacaac
      301 ctcgacaggt ttgaggaaat attcatctcg atggacgttg atgatgttgg tcgtgaagcc
      361 gcaagggaaa tcgtaagccg actcggtgaa catcgttgcc gtcttgttac tctgccgtac
      421 aaagacatca acgaatgcct gatgaacggt gttaccgagg atgaaatctg gcagtacatc
      481 ggcacggcat cctacttcga tcctgaagaa ctctacagtg cgcgagagtt ttaccaggac
      541 actatcaacg ctttctacgg caagcagcag tatctgttta atccaccgtg ggaatctctg
      601 gcagataaat tccagttccg tgaggcagag ttgacgctgg tcaatggtgt gcacggtcac
      661 ggaaaggcat gcccactgaa tgagcctatt cttttagctg atgggacatg gactactcac
      721 gggaatgtaa aaattggcga tcaggtggcg tcagtagacg gcaatccgtc aactgtcact
      781 gggatattcc cgcagggtgt tagagatgtt taccgagtca catttgaag
//
LOCUS       DAJTYH010000301          799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTYH010000301 DAJTYH010000000
VERSION     DAJTYH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..520)
                     /locus_tag="OO609_004231"
     CDS             complement(<1..520)
                     /locus_tag="OO609_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6635386.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQG"
     gene            complement(520..>799)
                     /locus_tag="OO609_004232"
     CDS             complement(520..>799)
                     /locus_tag="OO609_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6635387.1"
                     /translation="YGKHTILVCRLLPFVPFDPISYAAGLTSIRFRSFFIATGLGQLP
                     ATIVYSWAGSMLTGGTFWFVTELFILFALTVVIFMAKKIWLERQKRND"
BASE COUNT          215 a          221 c          212 g          151 t
ORIGIN      
        1 gcccctgagc gggaatgtcc atggcagaat taaaacgagc cgacaggttt tgcaggccga
       61 tcaatgccgt cagttcggtg agcgcctgag cgtcaaaatg cgcagccagt cgggtacgca
      121 gggcatcatc gactgttggc ggtgtgacgc ttgcggcttc ggcgtactcc agcgccagcc
      181 gttcttcatc gctaaagagc gggctttgcc gccagtcagc cactgccagc agtttatcgg
      241 tgctgccggt acgctcggcg actttcatac tggtgatatc cacgcaaaac tcacacaggc
      301 acatttgcgc aatgcgcgcg ctgacaagcg atcgcactac cggatcgagc ggtgagcgtt
      361 tgcgctccag ccacccaaca aacattgaca ccaaataaaa gataaacggg atccgccccc
      421 accagcgaat cgggcttagc acctcgccgt aatggcgacg atgcagccac gcctgcggac
      481 gtaaaatgaa aggaattttg ctaagcggcg gtaaacccat cagtcattcc tcttctggcg
      541 ctcaagccat attttcttcg ccataaaaat caccacggtc agggcaaaca gaataaacag
      601 ttcggtgaca aaccagaaag taccgcctgt taacatgctg cctgcccagg aataaacaat
      661 agtcgccggt aattgaccaa gcccggtggc gataaaaaac gagcgaaaac gtattgaagt
      721 cagaccggca gcatagctga ttggatcgaa agggacaaaa ggcaataacc gacagaccag
      781 gatggtgtgt ttgccgtag
//
LOCUS       DAJTYH010000302          771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTYH010000302 DAJTYH010000000
VERSION     DAJTYH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>771
                     /locus_tag="OO609_004233"
     CDS             <1..>771
                     /locus_tag="OO609_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6635388.1"
                     /translation="DTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFI
                     IFGVSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVS
                     GIWALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAK
                     TTSVVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMD
                     LTPTVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGL"
BASE COUNT          140 a          207 c          193 g          231 t
ORIGIN      
        1 cggataccgc cgccgttgcc gctctactgg tgccgatgat gcgcagcgcc aactacccgg
       61 tcaaccgggc ggcggggctg attgcctctg gcggcattat cgcgccaatt attccccctt
      121 ccattccgtt tattatcttc ggtgtttcca gcggattatc catcagtaag ctgtttatgg
      181 cgggcattgc ccctggcata atgatgggcg caacgctgat gcttacctgg tggcggcagg
      241 ccagtcgcct taatttaccc cgccagcaaa aagcaacgat gcaggaaatc tggcactctt
      301 ttgtctccgg tatctgggcg ctgtttcttc cagtcatcat tattggcggg tttcgctccg
      361 gtctgtttac ccctaccgaa gccggggccg ttgctgcttt ctatgcgctg tttgtcgcca
      421 cagttattta ccgtgaaatg accttcgcca cactttggca tgtactcatt ggcgcggcga
      481 aaaccacctc agtggttatg tttctggtgg cctcggcaca agtttccgcc tggctgatta
      541 ccattgctga actgccgatg atggtttctg atttactgca accgttggtc gattcaccgc
      601 gtctgctgtt tatcgtcatt atggtggcta tcctgattgt cggcatggtc atggatttaa
      661 cgccgacggt attaattctt accccggtat taatgccttt agttaaagag gcaggaatcg
      721 atccgattta tttcggtgtc atgtttatca ttaactgctc aatcggctta a
//
LOCUS       DAJTYH010000303          745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTYH010000303 DAJTYH010000000
VERSION     DAJTYH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>745
                     /locus_tag="OO609_004234"
     rRNA            <1..>745
                     /locus_tag="OO609_004234"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          182 a          171 c          240 g          152 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggtgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaat
//
LOCUS       DAJTYH010000304          742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTYH010000304 DAJTYH010000000
VERSION     DAJTYH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            20..562
                     /locus_tag="OO609_004235"
     CDS             20..562
                     /locus_tag="OO609_004235"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635389.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
BASE COUNT          216 a          142 c          182 g          202 t
ORIGIN      
        1 taacaccaaa ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag
       61 aactcagcat gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga
      121 tggtgtgaca gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc
      181 ctccggcggg gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc
      241 cactttgctt attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg
      301 agcaaaaagc ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg
      361 ccgttatctg agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa
      421 cattacagca gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat
      481 ggtgaatgaa gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag
      541 catgtcaggc tggaacctgt agagtcggtc ttcacttttt aggccagtgc gtaagtgtta
      601 gtgtcagctc ctgccatttc agcagaggaa attattctgc tgatgaaagc tttggcatca
      661 ggattcagcc tcaaacgtat gcgtggtcgt cagaggcagt taggtcagaa gcaataattt
      721 tagacagtga gaccatgtgc at
//
LOCUS       DAJTYH010000305          725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTYH010000305 DAJTYH010000000
VERSION     DAJTYH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..429
                     /locus_tag="OO609_004236"
     CDS             <1..429
                     /locus_tag="OO609_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6635390.1"
                     /translation="SGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDN
                     GKSGEKFLQELCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHA
                     AMNTLLGERVMDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            444..>725
                     /locus_tag="OO609_004237"
     CDS             444..>725
                     /locus_tag="OO609_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6635391.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT          179 a          151 c          228 g          167 t
ORIGIN      
        1 agcggtaagc ccggcaccgg aaaaaatcat cttgcagccg cgattggcaa ccggctgatg
       61 gcgaaggggc gtagcgtgat tatcgtcacc gtgtccgatg tcatgagcgt gttgcatgag
      121 agctacgaca acggcaaatc cggtgaaaaa tttttacagg agctttgtgg tgttgacctg
      181 ctggtcctgg atgaaattgg catgcagcgg gatacgaaaa acgagcaggt ggtactgaac
      241 cagattgttg atcgccggac ggcatcgtta cgcggtgtgg ggatgctgac aaatattaac
      301 catgcagcga tgaatacact tctcggcgag cgggtgatgg atcgcatggt catgaacggc
      361 gggcgctggg tgaattttaa ctgggagagc tggcgtccga atgttagcca ttcgagggtt
      421 gttaagtagt ttcaggagga tttatggcga aaccttttac tcccgaacag cgggaagaac
      481 tgaagacgcg aattgtggaa ctcgtgcatc aggacggtcg ggtcacgatt cggcagttgt
      541 ccgatgaaac aggtatcagt cgtgcgtctg tcggtcgctt atgcatagaa ctggtcgcaa
      601 gtggtgatgt atataattct ggctacggct tattcccgtc tgaacaggct cgcaaggact
      661 ggcaaagcgc ccgcaaaaaa ctctcgagag taaaggtgag gaaaccggtt gttgttgatc
      721 cggac
//
LOCUS       DAJTYH010000306          715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTYH010000306 DAJTYH010000000
VERSION     DAJTYH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            57..715
                     /locus_tag="OO609_004238"
                     /pseudo
     CDS             57..715
                     /locus_tag="OO609_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          193 a          172 c          179 g          171 t
ORIGIN      
        1 gccttccacg ccggatatcg gtttcttttt tcacagacca aagtatcatt tgacctgtga
       61 aacgattaca ggcatttaaa ttccagttaa gaccaggtgg tcaacaggag tgtgaaatga
      121 ggcgcttcgc aggcgcatgt cgtttcgttt tcaatcgtgc tctggcacgt cagaatgaga
      181 atcataaggc cggtaataaa tacatccctt acgggaaaat ggcttcctgg ctggttgagt
      241 ggaaaaatgc cactgaaacg caatggctta aagattctcc ctcacagcca ttgcaacagt
      301 cactgaaaga ccttgagcgg gcttacaaaa acttcttccg gaagcgggct gcttttcccc
      361 gattcaaaaa gcggtgacag aatgatgcat tccgctaccc gcagggcgtt aagctcgatc
      421 aggaaaacag ccgtattttt ctgccgaaac tgggctggat gcgctaccgg aatagccgtc
      481 aggtcacggg tgttgtgaaa aatgtcactg tcagccagtc ctgcggtacg tggtacatca
      541 gtattcagac agaaagtgaa gtatcaactc cggttcaccc ttcagcatca atgatcgggc
      601 tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt gagcctgtaa
      661 acagttttca aaaaaaccag aagacgctgg cgagacttca gcgacagtta agccg
//
LOCUS       DAJTYH010000307          714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTYH010000307 DAJTYH010000000
VERSION     DAJTYH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>714
                     /locus_tag="OO609_004239"
     CDS             <1..>714
                     /locus_tag="OO609_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR6635392.1"
                     /translation="GEAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFD
                     DDLAAQTTQASNRIRGLLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKK
                     LAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEV
                     ALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRR
                     SGSSIRGEHPSRRGNKALKR"
BASE COUNT          145 a          241 c          190 g          138 t
ORIGIN      
        1 ggtgaagcta aaactgatgc tcgtgacgct gccatcattg ccgaagctgc ccgtaccctg
       61 cctcacgcgc tacgcacgct gaaactggct gacgagcaaa tcgccgaact ctccatgctc
      121 tgcggcttcg atgatgatct tgccgcacag acaacgcagg ccagcaaccg tatccgcggc
      181 cttctgaccc agatacatcc ggcaccggag cgcgttctcg gtccgagact tgagcacccg
      241 gcggtactcg atcttctcca gcgatatccc tcaccagaaa aactcgcttc gctgggtgag
      301 aagaagctgg cagcccagct ctgcaaactt gcgcctcgtc tgggtaaacg ccttgcagca
      361 gacatagctc aggcactggc cgaacaaacc gtcgtcgttc ccggcacgaa tgccgctgcc
      421 gtagtactgc cacgtctggc actccagctc atcacgctgc gtaagcaaag agacgaggtg
      481 gcgcttgagg tagaacagcg agttcttgct caccctcttt acccggtcct gaccagtatg
      541 cccggagtcg gtgtcaggac cgcagccaga ctcctcaccg aggtcgcctg ccgcgccttc
      601 gcctctgccg cacatctcgc tgcttatgct ggccttgcgc cggtaactcg gcgatccggc
      661 tcgtcaatac gcggtgagca tccctcgcga cggggtaata aagctctcaa acgg
//
LOCUS       DAJTYH010000308          704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTYH010000308 DAJTYH010000000
VERSION     DAJTYH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..429
                     /locus_tag="OO609_004240"
     CDS             <1..429
                     /locus_tag="OO609_004240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635393.1"
                     /translation="RQKSLLKIGAVETGICVLRRSDIARHTEQGILVIGGAVRQKKFD
                     PLSVDLPDWLSETLWHEWVQFRQALRKPIRTELGANGAIRELEKFRQQGFTPEQVIRH
                     SIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            436..>704
                     /locus_tag="OO609_004241"
     CDS             436..>704
                     /locus_tag="OO609_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6635394.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQA"
BASE COUNT          172 a          179 c          213 g          140 t
ORIGIN      
        1 cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt cttgcggcgc
       61 agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc tgttcgccag
      121 aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac actctggcat
      181 gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct gggcgctaac
      241 ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga gcaggtgatt
      301 cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt tcggcctgag
      361 acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc gccaggcttc
      421 agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg tatccgcaga
      481 ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg cgagtggcaa
      541 cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca gttgcgggtg
      601 gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc gtttgcgaat
      661 taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggc
//
LOCUS       DAJTYH010000309          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTYH010000309 DAJTYH010000000
VERSION     DAJTYH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..695
                     /locus_tag="OO609_004242"
                     /pseudo
     CDS             1..695
                     /locus_tag="OO609_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          162 a          172 c          209 g          152 t
ORIGIN      
        1 gttgcccggc aacatggtgt agcagccagc cagttatttc tctggcgtaa gcaataccag
       61 gaaggaagtc ttactgctgt ggccgccgga gaacaggttg ttcctgcctc tgaacttgct
      121 gccgccatga agcagattaa agaactccag cgcctgctcg gcaagaaaac gatggaaaat
      181 gaactcctca aagaagccgt tgaatatgga cgggcaaaaa agtggatagc gcacgcgccc
      241 ttattgcccg gggatgggga gtaagcttag tcagccgttg tctccgggtg tcgcgtgcgc
      301 agttgcacgt cattctcaga cgaaccgatg actggatgga tggccgccgc agtcgtcaca
      361 ctgatgatac ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg
      421 gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga
      481 tcaatgccaa acgtgtttac cggctcatgc gccagaatgc gctgttgctt gagcgaaaac
      541 ctgctgtacc gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc
      601 agcgatggtg ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca
      661 cgttcgcgct ggactgctgt gatcgtgagg cactg
//
LOCUS       DAJTYH010000310          686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTYH010000310 DAJTYH010000000
VERSION     DAJTYH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..268
                     /locus_tag="OO609_004243"
                     /pseudo
     CDS             1..268
                     /locus_tag="OO609_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          213 a          132 c          133 g          208 t
ORIGIN      
        1 gccggaagag gaatgctgtc cggcctgtgg tggtgaactc agtccactgg gatgtgatgt
       61 gtcagagcag ctggagctta tcagcagcgc ctttaaggtt atcgaaaaac aacgccctaa
      121 actggcctgt cgccggtgcg accatatcgt gcaggcaccg gtgccttcaa aacccattgc
      181 acgcagttat gccggagcgg ggcttctggc ccatgttgtt accggaaagt atgcagacca
      241 tctgccatta taccgccaaa gtgatctact attccacaca gctatttagc tctctttttg
      301 ttgagctccc atgattcttt catttattgt tactaattat tttatgttat catgatgtta
      361 gaacacgctc tgctctgtgt ctctatttta tctatcatat tctgaggatt aactacataa
      421 caaggcgcaa gatatatata caaaaacaaa taataccgtt gcaataattc cttttagtaa
      481 aggaggtgtg attaatttca ggggatttca gagtaaaaat tgtacaacat attaaaatat
      541 gttcatttcg ttataataca cacaaggaaa tattaagtta gataatatat tctaaataac
      601 catagataaa aacgcacata atggggaata ttttatgaaa atacctgaag cagtaaatca
      661 tattaatgtt cagaataata ttgatc
//
LOCUS       DAJTYH010000311          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTYH010000311 DAJTYH010000000
VERSION     DAJTYH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..683
                     /locus_tag="OO609_004244"
                     /pseudo
     CDS             1..683
                     /locus_tag="OO609_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          170 a          162 c          216 g          135 t
ORIGIN      
        1 aatattgcga caggcggcgt tctggaacgc atccgccgac tgaccccgca acatgtaacc
       61 gccccattca gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt
      121 agcgaggaga tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct gaatcgctca
      181 ggcatccagc cgttgcaccg taaatgctcg tttgcgaatt accaggtgca gaacgacggc
      241 cagcgatacg cgttaagcca ggcgaaatcc atcgccgatg aactgatgac cgggtgtaca
      301 aattttgcgt tcagcggaaa acctgatacc gggtagaatc acttagcggc agctatcggg
      361 aatcgcctgc tgaaagacgg tcagacagtg attgtggtta ccgtggctga tgttatgagc
      421 gccctgcacg ccagctatga cgacgggcag tcaggcgaaa aatttttgcg ggagctgtgc
      481 gaagtggatc tgctggttct tgatgaaatt ggcattcagc gcgagacgaa aaacgagcag
      541 gtggtgctgc accagattgt tgatcgccgg acagcgtcga tgcgcagcgt ggggatgctg
      601 acaaacctga actatgaggt gatgaaaaca ttgctcggcg agcgggtgat ggatcgcatg
      661 gtcatgaacg gcgggcgctg ggt
//
LOCUS       DAJTYH010000312          678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTYH010000312 DAJTYH010000000
VERSION     DAJTYH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..539
                     /locus_tag="OO609_004245"
     CDS             <1..539
                     /locus_tag="OO609_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6635395.1"
                     /translation="LKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNFASD
                     TIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDY
                     RLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTIT
                     DKSSYNAIEWPASPEGSS"
     gene            563..>678
                     /locus_tag="OO609_004246"
     CDS             563..>678
                     /locus_tag="OO609_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6635396.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVA"
BASE COUNT          198 a          149 c          174 g          157 t
ORIGIN      
        1 atttaaaaaa tattactgcg ggtaatccga aaacagcaga acaatatcag atgacaaaac
       61 gatattcggt cacctggctt ttttcagaag acggaaaaaa ctggtatgaa gagctgaaga
      121 atttcgccag cgacacaata aaaatagctt acaccggaga tggtcgcgtg gtgtgggtcg
      181 gtaaggatgt gacaggcatc gagccacgca atgccagcgt tatcgaagtt ccggatatta
      241 ccgctaaccg acggattacc gcgccgggtt actggtttta ccgcaatgat gaatttgtct
      301 ttgactacag actcaaagcg gaagatgagc gtgatgccct tctggctcag gtcagtgccc
      361 ggacagggga atgggaagaa gacctgctgc tggggttaat cagcgacgaa gataaagaaa
      421 agctgaaagc ctgtcgtatt tacgcgaaat cgctgcaggc gatggatttc agcaccatca
      481 ctgataaatc ctcatacaac gccattgaat ggcccgcctc tccggaaggt tcttcctgat
      541 ttaatttatc gcgagaaaaa caatgtctgt agtgatatca ggtgcgctga ctgatggcgc
      601 aggtatcccc atgtccggat accatattat tctgaaatcc cgggtaaaca ccccggaagt
      661 ggtgatgaac actgttgc
//
LOCUS       DAJTYH010000313          678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTYH010000313 DAJTYH010000000
VERSION     DAJTYH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..266
                     /locus_tag="OO609_004247"
     CDS             <1..266
                     /locus_tag="OO609_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6635397.1"
                     /translation="TLSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAI
                     HSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            224..>678
                     /gene="bglJ"
                     /locus_tag="OO609_004248"
     CDS             224..>678
                     /gene="bglJ"
                     /locus_tag="OO609_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6635398.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRT"
BASE COUNT          190 a          138 c          171 g          179 t
ORIGIN      
        1 tcactctttc agagcgcaag gtactgcggc tattaggtaa aggatggggc atcaaccaga
       61 tagcttcatt gcttaagaaa agtaataaaa ctatcagcgc ccaaaaaaac agtgcgatgc
      121 gtcgactggc aattcacagc aacgctgaaa tgtatgcatg gataaatagc gcgcagggtg
      181 caagagaact taacttgcct tctgtttatg gagatgccgc agaatggaac acagccgaat
      241 taagaagaga aatgtcgcac tcatagaaaa atgcgtcatg agtagtatcg gtattgagag
      301 tttattcaga aagtttgcgg gcaaccctta taagctccat acctatacca gtcaggagtc
      361 atttcaggat gccatgtcgc ggatctcgtt tgcggcggtc attttttctt tttctgccat
      421 gagaagtgag cgcagagagg gattatcttg cctgactgag ctggcgatta agtttccgcg
      481 tacccggcgt ttagttattg tggatgatga tattgaagct cggctgattg gttcattgtc
      541 gccatcaccg ctggacggtg tattaagtaa agcgtcaacg ctggagattt ttcatcagga
      601 actctttttg tcattaaatg gtgtacgtca ggcgaccgac cgactgaaca atcagtggta
      661 cattaaccaa agccggac
//
LOCUS       DAJTYH010000314          676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTYH010000314 DAJTYH010000000
VERSION     DAJTYH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(86..511)
                     /locus_tag="OO609_004249"
     CDS             complement(86..511)
                     /locus_tag="OO609_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635399.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(508..>676)
                     /locus_tag="OO609_004250"
     CDS             complement(508..>676)
                     /locus_tag="OO609_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635400.1"
                     /translation="REIRLIKRLQQFVPDTLPVVRKASWHCRQCHHDYYGEQYCTHCQ
                     TGRFSEEGGAE"
BASE COUNT          169 a          203 c          152 g          152 t
ORIGIN      
        1 tcacgaacgg ggtgggtgcg aaacaccaga taaaatacag gttggcaata cctctgaacg
       61 ccctgagcag gtcgggcatt tcggattaag tactcgcacc tccgcagtcc tcaaacaagt
      121 ctggctggta gctgtaaaca gacttcgtac ataccgctct gcaataaatc cccgtaccac
      181 aggcctcaca gaacttcttc ccatgaaaaa actgctcaca catcacacaa tgccactcca
      241 gcacgaccgg taatggcgat aaaaacatcg ccatatcctc aatgtaaggg tgggacttct
      301 ccggattcag caccacacag tccgccttct gttgctcact cagggcatgt aaatcatgct
      361 caaaccacgc cccctgagca tctgtctgca gaatcaaccg gccatggcag gaaaggcaga
      421 aacactgccg gatatttctg ctgagcgcag agtcggcact gataatgtgc tcacccggcg
      481 tgatccccag ccccgttttt ataccgttca ttctgctccc ccctcctcac tgaagcgccc
      541 ggtctggcag tgtgtgcagt actgctcccc ataataatcg tggtgacatt gcctgcagtg
      601 ccagctggct ttacgtacta cgggtaaggt atccgggacg aattgctgca gacgtttaat
      661 caaccgtatt tctctg
//
LOCUS       DAJTYH010000315          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTYH010000315 DAJTYH010000000
VERSION     DAJTYH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            22..655
                     /locus_tag="OO609_004251"
                     /pseudo
     CDS             22..655
                     /locus_tag="OO609_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          197 a          163 c          166 g          129 t
ORIGIN      
        1 cagaacaaca caggtgctcc aatgaaaaaa agaaatttta gcgcagagtt taaacgcgaa
       61 tccgctcaac tggttgttga ccagaaatac acggtggcag atgccgccaa agctatggat
      121 gttggccttt ccacaatgac aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa
      181 acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct caggaaaaag
      241 ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct cttgatgtca
      301 gactccctga acagttctcg ataatcggga aactcagagc gcattatcct gtggtcacac
      361 tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac cgtcctgaaa
      421 aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat ggcatcagcc
      481 acggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc taccagatgg
      541 gacgctggct tgctggcagg ctcatgaaag agctggggct ggtcagctgt cagcagccga
      601 ctcaccggta taaacgtggt ggtcatgaac atgttgctat ccctaactac cttga
//
LOCUS       DAJTYH010000316          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTYH010000316 DAJTYH010000000
VERSION     DAJTYH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            18..491
                     /locus_tag="OO609_004252"
     CDS             18..491
                     /locus_tag="OO609_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6635401.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTYH010000317          625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTYH010000317 DAJTYH010000000
VERSION     DAJTYH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>625
                     /locus_tag="OO609_004253"
     CDS             <1..>625
                     /locus_tag="OO609_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6635402.1"
                     /translation="LPELLERIGAYHQGRELTRHAVLLMLHVFIRSSELRFARWSEID
                     FTNRVWTIPATREPIIGVRYSGRGAKMRMPHIVPLSEQSIAILKQIKDITGNNELIFP
                     GDHNPYKPMCENTVNKALRVMGYDTKKDICGHGFRAMACSALMESGLWAKDAVERQMS
                     HQERNTVRVAYIHKAEHLEARKAMMQWWSDYLEACRESYAPPYTIGK"
BASE COUNT          161 a          155 c          172 g          137 t
ORIGIN      
        1 ggctgcctga actgcttgaa cgtattgggg catatcatca gggccgtgaa ctgacccggc
       61 atgccgttct gctgatgctg catgtgttca ttcgctccag tgaactgcgt ttcgcccgct
      121 ggtcagagat tgatttcaca aaccgagtct ggacgatacc cgcgacgcga gaacccatta
      181 ttggcgtgcg ttattccggc cgcggggcaa aaatgcgaat gccgcatatc gtccccctct
      241 cagaacagtc catcgccatt ctgaaacaga ttaaggatat caccggtaat aatgaactga
      301 tcttccccgg cgaccataac ccgtataagc caatgtgtga aaacacggtc aataaggcac
      361 tgcgggtgat gggttacgac acgaaaaagg atatctgcgg tcacggcttc cgggcaatgg
      421 catgcagtgc gctgatggaa tcgggtttat gggcaaagga cgcagtagaa cgccagatga
      481 gtcatcagga gcgcaatacc gtgcgcgtgg cttatattca taaggcagag cacctggaag
      541 cccgcaaagc gatgatgcag tggtggtcgg attatctgga agcatgccga gaatcttatg
      601 caccgcctta tacaattggt aaaaa
//
LOCUS       DAJTYH010000318          600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTYH010000318 DAJTYH010000000
VERSION     DAJTYH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>600
                     /locus_tag="OO609_004254"
     CDS             <1..>600
                     /locus_tag="OO609_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR6635403.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLG"
BASE COUNT          151 a          174 c          170 g          105 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 taaaggttat tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca
      481 ctggatgata cctctcagaa agggagcgca atatgaagag ctcagaaaac tgggtaaagg
      541 cgatcatctg gtgaagctga aaaccagccc gcaggcacga aaaaagtggc cgggactggg
//
LOCUS       DAJTYH010000319          596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTYH010000319 DAJTYH010000000
VERSION     DAJTYH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>596)
                     /gene="ospC2"
                     /locus_tag="OO609_004255"
     CDS             complement(<1..>596)
                     /gene="ospC2"
                     /locus_tag="OO609_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6635404.1"
                     /translation="QKNISCVTNSSSSGISEKHLDHCADTVKSFLRKSIAAQSYSKMF
                     SQGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSEIIQKTHPLSSDERHLLSII
                     INSDFNFRHQSNANLSNNTLNIKSFDKIKSENIQTYKNTFSEDIEEIANHDFVFFGVE
                     ISNHQETLPLNKTHHTVDFGANAYIIDHDSPYGYMTLT"
BASE COUNT          188 a           87 c          116 g          205 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttg
//
LOCUS       DAJTYH010000320          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTYH010000320 DAJTYH010000000
VERSION     DAJTYH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(92..>594)
                     /locus_tag="OO609_004256"
     CDS             complement(92..>594)
                     /locus_tag="OO609_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6635405.1"
                     /translation="PIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYKAWLLW
                     CYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAELFGGE
                     GYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKAAFSKQ
                     SIAKVD"
BASE COUNT          151 a          157 c          113 g          173 t
ORIGIN      
        1 cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac aaaatcggca
       61 aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata ctttgctttg
      121 aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc tcactatcca
      181 gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg gaccagtttt
      241 tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc ccaccaaaaa
      301 gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt tcctgtgttt
      361 tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc cagtgggtta
      421 tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac caggctttat
      481 agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac gcaacctgac
      541 tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt ggtg
//
LOCUS       DAJTYH010000321          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTYH010000321 DAJTYH010000000
VERSION     DAJTYH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..98
                     /locus_tag="OO609_004257"
     CDS             <1..98
                     /locus_tag="OO609_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6635406.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO609_004258"
     CDS             156..512
                     /locus_tag="OO609_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635407.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAJTYH010000322          573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTYH010000322 DAJTYH010000000
VERSION     DAJTYH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..298)
                     /locus_tag="OO609_004259"
     CDS             complement(<1..298)
                     /locus_tag="OO609_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635408.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGD"
BASE COUNT          194 a          108 c          101 g          170 t
ORIGIN      
        1 cgtcgccgcc aggcgcttct gtaccatcaa aacgaacggc tgcttgagtg tacgttgccg
       61 ggcaattttc aagtttaata ctgaacttct gtggcgacgc cagactacct acacctgaaa
      121 atgcattttt actgacttta cccagtggga caatttgatc ggctgtatcg gaggtaactt
      181 cacaagtagt gtcgacaatt tcaccgacga agttaacaac accatcttct gccagtgcat
      241 gcccggccag cattagagat gccagagtga atgcaccgcc tataatatta cgcttcataa
      301 aaactccatt gatataatat atcctttacc atgaatcatc acttatatta ataagtgtgt
      361 ttttgattca aaggaatagt acaccaaaga gacggcttta tctaaaaata atattaatta
      421 cgcaaaaata aaatatagat ttattgttta aatattattt aaacagtaaa aaatcaagtt
      481 tgttatcaga cattaatgat aattcagaat atatttatat agaaagatag atgaatttat
      541 tgatgtcagt tataaaaaag ggccaccggt cgg
//
LOCUS       DAJTYH010000323          555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTYH010000323 DAJTYH010000000
VERSION     DAJTYH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..288)
                     /locus_tag="OO609_004260"
     CDS             complement(<1..288)
                     /locus_tag="OO609_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical efflux pump membrane protein ArsB"
                     /protein_id="HCR6635409.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFT"
     gene            complement(342..>555)
                     /gene="arsR"
                     /locus_tag="OO609_004261"
     CDS             complement(342..>555)
                     /gene="arsR"
                     /locus_tag="OO609_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6635410.1"
                     /translation="SRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRC
                     EQEKIQAIVRNLARQNCSGDSKNICS"
BASE COUNT          147 a          164 c          124 g          120 t
ORIGIN      
        1 ggtaaacagc aagcgaccgc gtccattgcc ccagcgtgaa acgtgcagcg ctgcccattc
       61 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcaataa acgccgccgt
      121 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatgaacca cgcccgtaac
      181 caacgccagt accgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
      241 gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcccgg
      301 ataaaacaca tgtgcaaatc catatgtgtt tagctaaatt tttaactgca aatgttctta
      361 ctgtctccgg aacagttttg tctggccagg ttgcggacaa ttgcctgaat cttttcctgt
      421 tcacatcgcc aggcttcatc aataattttc gccgcccatg ctggaatatg cggcgataag
      481 cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
      541 gccagatggc gggat
//
LOCUS       DAJTYH010000324          554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTYH010000324 DAJTYH010000000
VERSION     DAJTYH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
BASE COUNT          197 a          110 c           78 g          169 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaa
//
LOCUS       DAJTYH010000325          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTYH010000325 DAJTYH010000000
VERSION     DAJTYH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..453
                     /gene="ospC2"
                     /locus_tag="OO609_004262"
     CDS             <1..453
                     /gene="ospC2"
                     /locus_tag="OO609_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6635411.1"
                     /translation="TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSL
                     GNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFIS
                     NGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          203 a           73 c          108 g          164 t
ORIGIN      
        1 acagataact tcaaaaagat aagactcaga gatatatctt tggaggatgc cattaaagca
       61 tccaattatg aagaaattaa caataaggtg actgataaaa aaatggctca tcaagctctt
      121 gcttattctc taggcaatgc aaaatctgat atggcacttt atttactatc taaatttaat
      181 tttacaaaac aagatatcgc agagatggag aaaatgaaca acaatatgta ttgtgagctg
      241 tatgatgttg agtatttact tagtgaagat agtgccaact ataaagtgct agaatatttt
      301 attagtaatg gattggttga tgtaaacaaa agatttcaaa aagcaaatag tggggacact
      361 atgcttgaca atgcaatgaa aagcaaagat tcaaaaacaa ttgatttttt attaaaaaat
      421 ggagcggtat caggcaaacg atttgggagg tgacctaaaa tatatttagg ttgtgccctg
      481 aatctgacgt tgtcattttt gttatattga tcgatatagg ggatggtgca gaccgttccc
      541 ctgcaaca
//
LOCUS       DAJTYH010000326          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTYH010000326 DAJTYH010000000
VERSION     DAJTYH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            17..523
                     /gene="mug"
                     /locus_tag="OO609_004263"
                     /pseudo
     CDS             17..523
                     /gene="mug"
                     /locus_tag="OO609_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT          128 a          114 c          159 g          124 t
ORIGIN      
        1 ttagaaagga gtaaatatgg ttgaggatat tttggctcca gggttacggg tcgtgttttg
       61 cggtatcaac cctgggcttt catccgccgg gactggtttt ccctttgctc atccggcaaa
      121 tcgcttctgg aaggtgatat atcaggccgg gtttaccgac cgtcagttga agccgcagga
      181 ggcacagcat ctgctggatt atcgttgtgg catcaccaaa ctggtagacc gtccaacggt
      241 gcaagccaat gaagtttcaa agcaggagct gcacgcaggt gggcgtaagc tgattgaaaa
      301 aattgaagat tatcagccgc aggcgttggc tattctgggc aaacaagcat atgaacaggg
      361 attcagctag cgcggtgcac agtgggggaa acaaacgctc accattggtt cgacgcagat
      421 ttgggtgctg ccaaatccca gcggtttaag tcgcgtttca ctggagaaac tggttgaagc
      481 gtatcgcgag ctggaccagg cgctggtagt gcgtgggcga taaaa
//
LOCUS       DAJTYH010000327          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTYH010000327 DAJTYH010000000
VERSION     DAJTYH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            23..524
                     /gene="fepE"
                     /locus_tag="OO609_004264"
                     /pseudo
     CDS             23..524
                     /gene="fepE"
                     /locus_tag="OO609_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT          146 a          111 c          115 g          152 t
ORIGIN      
        1 actcattttc aggttatacc ccatgtcatc attgaatatt aaacagggaa gtgacgctca
       61 tttttctgag tatcctctgg cgtcgcccag taataatgaa attgatttac ttaatctaat
      121 cgaagtttta tggcgggcca aaaaaacggt catggcggtc gtttttgcat ttgcctgcgc
      181 aggtttactg atctctttca tcctgccgca aaaatggacc agctcagccg ttattactcc
      241 cgccgaagcc attcagtggc aggacctgga gaaaaccttt accaaactcc gcgttcttga
      301 tctcgatgtt aatatcgacc gtggcggtgc atttaacctg tttatcaaga agtttcagtc
      361 tgttagcttg ctggaagagt acctgcgttc atcaccttat gtgatggact aattaaaaga
      421 ggcgaaaatc gatgaactgg atttgcatcg cgctattgtt gcattgagcg aaaaaatgaa
      481 agcggttgat gacaatgcta gtaagaaaaa agatgaaccg tcac
//
LOCUS       DAJTYH010000328          512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTYH010000328 DAJTYH010000000
VERSION     DAJTYH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..365)
                     /locus_tag="OO609_004265"
     CDS             complement(<1..365)
                     /locus_tag="OO609_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel"
                     /protein_id="HCR6635412.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLV"
BASE COUNT          173 a           99 c          110 g          130 t
ORIGIN      
        1 accagcgtaa aaatgcaaaa tccaatacta aatttcagcc agggataaaa atgcagggtg
       61 taaattagcg ctatcaacaa caaaatgata ctgattgccc atgcgagctt tgcccggaac
      121 agtaagccaa tggagttcag gacaagaaag acgccgagca taaatagtgg ggcgtttgcc
      181 agcaaggaga gatccaactc tgaaaatgct ttgatatgga aaatatcaag caaattgacc
      241 gacataccat agattgtttt aaaaataagc aatccattta aaaagacggc cagcgcgaga
      301 atgtcgtgcc gtagggtcac ccataaattt gttgctgttt gtttgaatgt agcccagtga
      361 ctcactgata cctctgtaac cacaattttg tttaattatc ttagcgtgca cattttctga
      421 acatacatgc agcgcgtgaa tgtgttaaac aatagtagat gactttatcc gcagtaatca
      481 aaggttttga gtgagcaact ctgcgtcagg aa
//
LOCUS       DAJTYH010000329          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTYH010000329 DAJTYH010000000
VERSION     DAJTYH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>501)
                     /locus_tag="OO609_004266"
     CDS             complement(<1..>501)
                     /locus_tag="OO609_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6635413.1"
                     /translation="LMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWL"
BASE COUNT          133 a          163 c          107 g           98 t
ORIGIN      
        1 cagccatgcc gccactacca gcgcactgcg cgataatccc aatgcgcaat ggaccagaac
       61 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
      121 ttccggaacc accagatcca acatcggtac acagaaatag agtcgatctt ttgtcgctcg
      181 tccacgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg
      241 aaacgccctt aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
      301 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
      361 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgcacccacg
      421 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
      481 catcatcatt agctccatca a
//
LOCUS       DAJTYH010000330          497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTYH010000330 DAJTYH010000000
VERSION     DAJTYH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>497
                     /locus_tag="OO609_004267"
     CDS             <1..>497
                     /locus_tag="OO609_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6635414.1"
                     /translation="NQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVGRSGGGK
                     STLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVGLGLKGQ
                     WRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPLGALDAL
                     TRLEM"
BASE COUNT          105 a          121 c          164 g          107 t
ORIGIN      
        1 aaccagggca caccattgtt gctcaatgca gtaagcaaac attacgcgga aaatatcgtc
       61 ctgaaccaac tggatttaca tattccggca ggtcagtttg tggcggtggt gggccgcagc
      121 ggtggtggta aaagtaccct gctgcacctg ctggccggtc tggaaacgcc aaccgcaggc
      181 gatgtgttag cgggcaccac accgctggct gaaattcagg atgatacgcg aatgatgttt
      241 caggatgcgc gtctgctgcc gtggaaatcg gtgattgata acgttgggtt aggccttaaa
      301 ggtcagtggc gcgatgccgc gcgtcgggcg ttggctgcgg caggactgga gaatcgcgcc
      361 ggggaatggc ctgccgcact ttctggcggg cagaaacagc gtgtggcgct ggcaagagcg
      421 ttgattcatc gaccgggttt attgttgctt gatgaaccgc tcggggcact ggacgcctta
      481 acgcgactcg agatgca
//
LOCUS       DAJTYH010000331          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTYH010000331 DAJTYH010000000
VERSION     DAJTYH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>493)
                     /gene="celF"
                     /locus_tag="OO609_004268"
                     /gene_synonym="chbF"
     CDS             complement(<1..>493)
                     /gene="celF"
                     /locus_tag="OO609_004268"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6635415.1"
                     /translation="IKRYHELPVSELWLVDVEGGKAKLDIIFDLCQRMIDNAGVPMKL
                     YKTLDCREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTI
                     PVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKMFIHD
                     VLML"
BASE COUNT          143 a          129 c           98 g          123 t
ORIGIN      
        1 tcagcatcag aacatcgtga ataaacatct tcatgccgat cggaatatta cacacgccga
       61 taaagcgttt aaatccggta tgacgataaa cggcttcagt aaccattccc gccgggttag
      121 tgaagttaat cacccatgca ttcggacaaa gttcttcgac atcttttacg atgtcaaaaa
      181 tcaccggaat ggtacgcaga cctttaaaca aaccgcccgc gccgttggtt tcctgaccaa
      241 gataaccatg acttaatgga atacgttcat ccagttcacg cgccggtaat tggccaacgc
      301 gcagttgggt agtaacgaaa tcagcatctt tcaatgcttc gcggcaatcc agcgttttat
      361 aaagcttcat cgggacgcca gcgttatcaa tcatccgttg gcagagatcg aaaataatat
      421 ccagtttagc tttgccaccc tcgacatcca ccagccataa ttcgctgacc ggcaattcgt
      481 gataacgctt aat
//
LOCUS       DAJTYH010000332          491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTYH010000332 DAJTYH010000000
VERSION     DAJTYH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>491
                     /gene="ospC4"
                     /locus_tag="OO609_004269"
     CDS             <1..>491
                     /gene="ospC4"
                     /locus_tag="OO609_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6635416.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYK
                     VLE"
BASE COUNT          194 a           59 c           86 g          152 t
ORIGIN      
        1 aaaactgatg ttccaatatt taacactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa ggattcagag agttttgtta taacaaaaat
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagtacaga taacttcaaa aaaataaaac tcagagacat atctttggag
      241 gatgcgatta aagcatctaa ttatgaagag attaacaata aagtgactga taaaaaaatg
      301 gctcatcagg cccttgctta ttctttaggc aataaaaaag ctgatatagc actttattta
      361 ctatctaaat ttaattttac aaaacaagat gtcgcagaga tggagaaaat gaaaaacaat
      421 agatattgta atctgtatga tgttgaatac ttacttagta aagatggtgc caactataaa
      481 gtgctagaat a
//
LOCUS       DAJTYH010000333          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTYH010000333 DAJTYH010000000
VERSION     DAJTYH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
BASE COUNT          167 a           92 c          105 g          124 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 gggggggg
//
LOCUS       DAJTYH010000334          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTYH010000334 DAJTYH010000000
VERSION     DAJTYH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(53..445)
                     /gene="iraD"
                     /locus_tag="OO609_004270"
     CDS             complement(53..445)
                     /gene="iraD"
                     /locus_tag="OO609_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6635417.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
BASE COUNT          126 a          118 c          121 g          115 t
ORIGIN      
        1 aatccagttg ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattgt gcttagctga
       61 cattctcaag cgtcgcactg cgccaaccgc cattatccca gaccagatca aacgttagcg
      121 gttcatcgtc gtggtacagc attatctcaa ggctgatcgc cagagtgtgg cagtacgctt
      181 ctttcaaatt aacctgcaac gccgcaatcc gtggctcatg aaataacagt gcagaacgca
      241 gctgacggca ataccagctc tggagatgtt tgtcgctcgg gttatgcact tgccactcgg
      301 gcatgcctga aacaagtaat gacggtaatg ccgaatgagg agaattaaaa agagtgagga
      361 gatcgctgca taccgattta cgcagcaatg acgttttatt gcctgaaatt tcaggtaaaa
      421 cagcctgaag tgattggcgc atcattttgc gcactcctga cgtttagcaa catatgcggc
//
LOCUS       DAJTYH010000335          478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTYH010000335 DAJTYH010000000
VERSION     DAJTYH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..131
                     /locus_tag="OO609_004271"
     CDS             <1..131
                     /locus_tag="OO609_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6635418.1"
                     /translation="GISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
BASE COUNT          109 a          135 c          122 g          112 t
ORIGIN      
        1 gcgggatttc cggccgcagt gcaaaaggaa aacgtatcca cacccgtgcc attcacaaca
       61 tcgacaccga tattaagctc aaccgcgcat tgtgggtgat ggcagaaacg ctgctggaga
      121 gcctgcgctg atgcagcatg tccctgaagg cgtgattcag gcaccagaat cacccgacgc
      181 cccggaggcg ctgccggggt gtcgttccat ttctgatgat gacctgtgcg cctggtgtac
      241 ccgtctgctt taccgtcccg gagaaatcag tctgtgcaga ctgagtatac aggcaatggc
      301 atctggccat ccgtctgtga ccggaacggt tacgcgtacg gctgtccgga atttcagccg
      361 aatatcattc atccctgata ttaccgggtc atcacggtca gtgatgaacg tgatgttgcc
      421 agctcccgac ctttactccc tctcatttat tcacattaca aggatttcat acatgaaa
//
LOCUS       DAJTYH010000336          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTYH010000336 DAJTYH010000000
VERSION     DAJTYH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..296
                     /locus_tag="OO609_004272"
     CDS             <1..296
                     /locus_tag="OO609_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6635419.1"
                     /translation="RWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALSSTL
                     DSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            268..>473
                     /locus_tag="OO609_004273"
     CDS             268..>473
                     /locus_tag="OO609_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6635420.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDE"
BASE COUNT          132 a          102 c          121 g          118 t
ORIGIN      
        1 ccagatggga actgaccgca gaacaggctg tcgcacagca gttaaaaaat caggtgtcaa
       61 aaggaaatct tatcgatacc ggattttgta tttttgccct tagtaagctg gcgatggctt
      121 tatccagtac gcttgactcc attcctttgt ccatgcaacg acagttcccg gatttaacgc
      181 cgcgtcatat tgatcatctg aaaatcctta ttgctaaggg agcgaatcag tgcgcgcgtg
      241 cgggggataa attaccggat ttactcgatg aatatatcag aacaacaact gagtaatatg
      301 atggtggcag tcactatcgc actgcagcca ctggtcaggg tattgccggt gacggctgta
      361 gaatgggccg atcaaaatta ttacctgcct aaagaatcgt catacggtga gggggaatgg
      421 aaaacgctgc agtttcaggt cgccatcatg aactgcatgg gcaatgacga gat
//
LOCUS       DAJTYH010000337          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTYH010000337 DAJTYH010000000
VERSION     DAJTYH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>472
                     /locus_tag="OO609_004274"
     CDS             <1..>472
                     /locus_tag="OO609_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6635421.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAJTYH010000338          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTYH010000338 DAJTYH010000000
VERSION     DAJTYH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..361
                     /locus_tag="OO609_004275"
     CDS             <1..361
                     /locus_tag="OO609_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6635422.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAJTYH010000339          468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTYH010000339 DAJTYH010000000
VERSION     DAJTYH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
BASE COUNT          141 a           79 c           81 g          167 t
ORIGIN      
        1 taggtttggt gattgattat tataaatatc atcacccctt aaggtattcc cttagagtta
       61 atctttaatt ggtctttaat taaatctttt atttaatact taaagttaat ctctaattgt
      121 gtctttaagt taactttaag taatactata agttaatctt taattgtgtc tttaagttaa
      181 ctttaagtta agcccctgta gggactagag accataatta agatgattgt gtcttattgc
      241 tattcactta tcgactgacc cagagcacac aggaagcatt cactttaagt agaacgcttg
      301 gtgtctgtaa ttggtcatcc cgcccctgaa aggatgcgat aagttacggt tagttgtcct
      361 agaagagaaa actttttgtg tcccttctcc ctatagtgag tcgtattaat tatccttgac
      421 aagtagagaa aaccatgagt caaaaacatg accacacagg tctgtagt
//
LOCUS       DAJTYH010000340          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTYH010000340 DAJTYH010000000
VERSION     DAJTYH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>465)
                     /gene="btsS"
                     /locus_tag="OO609_004276"
     CDS             complement(<1..>465)
                     /gene="btsS"
                     /locus_tag="OO609_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6635423.1"
                     /translation="KAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQASQLVQ
                     YLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQQLPA
                     FTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNA"
BASE COUNT          106 a          108 c          122 g          129 t
ORIGIN      
        1 tggcattggt taccggttga tacaaaccgg cattgtcttc gatctccagc atcaaatgtt
       61 gcccctcacg tcgggcgctg attgccactc gccctgtatc cagcagttgt gatgtcccat
      121 gtttaatggc gttttccact atcggttgca gggtaaacgc gggcaattgc tgctgggata
      181 attcttgcgg aatagcaatg ttgacctgca accgcgactg gaagcgcgcc ttttcaattt
      241 gcagataagc gttcacatgt tcaatttcgt cggcgagagt aacaaactcc gaaggccgct
      301 ttaagttttt gcggaaaaaa gtggaaagat actgcaccag ctggctggcc tgttcgctgt
      361 cgcggcggat caccgcttta atggtgttaa gcgcattaaa caaaaaatga ggattcacct
      421 gggcgtgaag cagtttgatt tctgactggg tgagcatcgc ttttt
//
LOCUS       DAJTYH010000341          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTYH010000341 DAJTYH010000000
VERSION     DAJTYH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
BASE COUNT          144 a           88 c           94 g          139 t
ORIGIN      
        1 ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc gatgtttacg gctaatatca
       61 gcgttgttta ttttacgtcc aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa
      121 aaaatccctc atcgtcatca ctgttttaaa atgactcact attttccaca gcaaatataa
      181 agtgtcagtt tgcgacgcga gcggcatttt aggacatatc ttccccggtt aacagtcctt
      241 gttacgtctg tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg
      301 ttaaatcggc aattcatcgc cgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc
      361 acttaattat tgtgaatttc agctggtgag ttaattaacg ggcgggttta aaaccattta
      421 tcaataatag tcaggaatag tcttatttac tttaaggata ttgat
//
LOCUS       DAJTYH010000342          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTYH010000342 DAJTYH010000000
VERSION     DAJTYH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>463
                     /locus_tag="OO609_004277"
     CDS             <1..>463
                     /locus_tag="OO609_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6635424.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTYH010000343          455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTYH010000343 DAJTYH010000000
VERSION     DAJTYH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
BASE COUNT          149 a           71 c           87 g          148 t
ORIGIN      
        1 atttcagagt tggagttcga atgttgacac ctgagtattt cctgctaaaa atagccctgc
       61 tttacggatg gttgtattgc aataacacga aagtagtaag agattaaagg tatctgtcag
      121 cgataactct gcaagtgatg acattttgta aaccaaataa tgtaaatcag caatgtatct
      181 tttcacttaa ttataacaaa tgtggtattc actaagaatc tgatacgagc ttacatcacg
      241 actattttag tctcatcgcg ggatacaggg atgcaccgat ggatctcagc acaccgtaat
      301 gcaatacctg aacaaataga acagtaaagt ggttcagtat taatgaaagc tcttattttc
      361 cgttgggaaa acttaaaata gatgagaagg ctttttgatt tcttatgtga tctcatttcg
      421 agtattgaga aatttgtgat ttttaaattt ttcaa
//
LOCUS       DAJTYH010000344          453 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTYH010000344 DAJTYH010000000
VERSION     DAJTYH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>453)
                     /locus_tag="OO609_004278"
     CDS             complement(<1..>453)
                     /locus_tag="OO609_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HCR6635425.1"
                     /translation="GTAGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTL
                     IPFFAFALGNTIDLTVIAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAAS
                     SSAGAAVATPVLIAEMVPAFKPMAPAATSLVATAVIVTSILVPILTSI"
BASE COUNT          131 a          133 c          111 g           78 t
ORIGIN      
        1 cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt cgctaccagc
       61 gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat cagcacaggt
      121 gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc atcgccaccg
      181 ccaatcaatt tatcggcgat aatcaacgga ataccggtca cgataattac tgccacaccc
      241 aacaggatcc ccagcaaacc agtctgggca atcacagtca aatcaatggt attgcccagc
      301 gcgaaggcaa agaatggaat cagcgtttgc accgctttgc tgaaaaattc tcgtaactca
      361 gggtcaagat tccccagggc aaagcccacc aggaacggta atactgcgcc gacgaaaaca
      421 tgcggttcaa acgaggcaat cccggcagtg ccc
//
LOCUS       DAJTYH010000345          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTYH010000345 DAJTYH010000000
VERSION     DAJTYH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>452
                     /locus_tag="OO609_004279"
     CDS             <1..>452
                     /locus_tag="OO609_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6635426.1"
                     /translation="PLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAG
                     YDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSPSVQDKPA
                     STDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRD"
BASE COUNT          133 a          110 c          108 g          101 t
ORIGIN      
        1 ccactgtatt acgaagcaaa aaatgacaaa gtaagaaaac gtctcggtat taaaggcggt
       61 ttttactggg ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt
      121 gacgatgcag gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt
      181 gttgatgacc tcccgccaga aggcgtattt gatacggaat tctgcaaccg ttacgaaaaa
      241 ggcggggaag atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa
      301 ccggccagca ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac
      361 atgctcctgc cagtttccgg tcaggaactg cccattcgct ggcttgcgca acacggcagc
      421 gaaaaaccag taacgcacgt ttcacgcgac ga
//
LOCUS       DAJTYH010000346          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTYH010000346 DAJTYH010000000
VERSION     DAJTYH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..329
                     /locus_tag="OO609_004280"
     CDS             <1..329
                     /locus_tag="OO609_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6635427.1"
                     /translation="KLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKRLDKCVFGEE
                     KPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRPVPELPEKYR
                     PKKPRE"
BASE COUNT          121 a          108 c          121 g           97 t
ORIGIN      
        1 aaaaactgac gattaaaaaa atgatcgatc tttatcaagc gaaatgcccg caggcgtcag
       61 cggagccgga gcattacgag gcgttgtttg tttacgcgca aaagcggctg gataaatgtg
      121 tgttcggcga agagaaacca gcctgtaagc agtgtccggt gcactgttac cagcctgcaa
      181 agcgtgaaga gatgaaacag attatgcgtt gggccgggcc gagaatgctg tggcgtcatc
      241 cgatcttaac cgtgcgtcat ctcatcgatg acaagcgtcc ggtgccagaa ctgccggaaa
      301 aatatcgtcc gaaaaaacct cgtgaatagt atgccggaca ggcagatctg tccggcaatt
      361 tgcagttaaa tcaatgccga tttatcaatt cccagccgct tcatccgtga cagcagggtc
      421 gtgcgtttca gccccagacg ttgcgca
//
LOCUS       DAJTYH010000347          446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTYH010000347 DAJTYH010000000
VERSION     DAJTYH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..446)
                     /locus_tag="OO609_004281"
                     /pseudo
     CDS             complement(1..446)
                     /locus_tag="OO609_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT          113 a           88 c           93 g          152 t
ORIGIN      
        1 tagccggata ttggtgttgt gaagtgagtt ggttattcaa agcgattata gctaactcta
       61 atcccttagc taatttttgt tgtttaaatg cgggtatcat gttgctacgg atgatatctc
      121 cagccagagc atcggttacc ttttcttcca gcccataacc tacctggatg cggacagtgc
      181 gatccgacta ggcaacaacg atcagtatcc catcattacg tttggcatct cctaaacgcc
      241 aattgtcaaa aactcttgta gcatattgtt caattgtctc atctttggtt gtggaaacaa
      301 ttaatacagc aatttgcact tgggtattat tgaaaataga ttctaacttg tgcgtcagtt
      361 ctttcttttc ttgtgagttt aagctactcg taagacccgt aacttgttgc tgtaattgtg
      421 ggacaatagt tggttgggct gcaaca
//
LOCUS       DAJTYH010000348          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTYH010000348 DAJTYH010000000
VERSION     DAJTYH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>444
                     /locus_tag="OO609_004282"
     CDS             <1..>444
                     /locus_tag="OO609_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HCR6635428.1"
                     /translation="INYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLTMQVA
                     RLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGAASWAYLG
                     KSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAA"
BASE COUNT           86 a          114 c          135 g          109 t
ORIGIN      
        1 atcaattatg aagatcgctg gttctggaag catccggggg ttaatccatt ctcggtggcg
       61 cgcgcagcat ggcaagatct cacttcggga cgggttattt ccggtggcag cacgctcact
      121 atgcaggttg ctcgtctgct tgatcctcac cccaaaacat ttggtggcaa aattctccag
      181 ctctggcgcg cgttgcaact ggagtggcat ttgtctaagc gtgaaattct gatcttgtat
      241 cttaaccgcg ctccgtttgg cggtacgttg caggggatcg gtgcggcaag ttgggcttat
      301 ctcggaaaat cgcctgcgaa tttaagctat tccgaggcgg caatgctggc ggtgttaccg
      361 caagtgccca gccgactgcg cccggatcgt tggccggagc gtgccgaagc cgcgcgtaat
      421 aaagtgctcg aacggatggc tgcg
//
LOCUS       DAJTYH010000349          441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTYH010000349 DAJTYH010000000
VERSION     DAJTYH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..269
                     /locus_tag="OO609_004283"
     CDS             <1..269
                     /locus_tag="OO609_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6635429.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>441
                     /gene="tnpB"
                     /locus_tag="OO609_004284"
     CDS             266..>441
                     /gene="tnpB"
                     /locus_tag="OO609_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6635430.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWA"
BASE COUNT          109 a          115 c          126 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg c
//
LOCUS       DAJTYH010000350          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTYH010000350 DAJTYH010000000
VERSION     DAJTYH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>438)
                     /locus_tag="OO609_004285"
     CDS             complement(<1..>438)
                     /locus_tag="OO609_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HCR6635431.1"
                     /translation="NGGNQQGFGGEGIETFLYVISGNITAKAEGKTFALSEGGYLYCP
                     PGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYEGMDDVILLDF
                     LPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT          118 a          107 c          102 g          111 t
ORIGIN      
        1 gttataaacc ccctgaccgg aaagaatata ggcaccatgt tcctgaacgt gtgtttcgat
       61 ataaccgtgg ctggcacctg gtgcaaaaga gaggatatgc atgttcatat caaaacctaa
      121 ctctttgggc agaaaatcca gcaggatcac atcatccata ccttcatagt gaatgcgttc
      181 cagttcgctg gcattgccag aaaacagcca cggtgcatgg ccttctaccg gaacatagcg
      241 gcgcttatat aaaaagattt ggctgtcttc ggcctgggcg ttaacaaacg tcattaagga
      301 gcctggtggg caataaagat agccaccttc gcttaaggca aatgttttgc cttcggcttt
      361 ggcagtgata tttccagaga tcacatacag gaacgtttca atgccttcgc caccgaagcc
      421 ctgttggttg ccaccgtt
//
LOCUS       DAJTYH010000351          437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTYH010000351 DAJTYH010000000
VERSION     DAJTYH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>437)
                     /gene="maeA"
                     /locus_tag="OO609_004286"
     CDS             complement(<1..>437)
                     /gene="maeA"
                     /locus_tag="OO609_004286"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6635432.1"
                     /translation="VGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIV
                     VTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPL
                     YMGWRNPRITDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKN"
BASE COUNT          111 a          119 c          106 g          101 t
ORIGIN      
        1 atttttttga gcaaagtctt caaactgcaa cagcacgtcc ggccagcgtt gtttcacagc
       61 ctggataaat tcatcaacga attcatagta ttcatcgtca gtgatacgcg gattacgcca
      121 gcccatatac agcggatcgt taagcagctg ttggttgttc gttccgacat ccagcaccac
      181 cggaagggta tacgccgggc tgatgccgcc acaggcggta tagagcgaca gtttaccgat
      241 cggaatgccc atcccgccga tgccctggtc accaagcccc agaatacgtt caccgtcagt
      301 caccacaatc actttaatat tatggttcgg cacgttttgc agaatatcgt ccatattgtg
      361 gcggttctgg taagagataa acacgccgcg tgaacggcgg tagatctcag aaaaacgctc
      421 acaggctgcg ccgacgg
//
LOCUS       DAJTYH010000352          435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTYH010000352 DAJTYH010000000
VERSION     DAJTYH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..302
                     /locus_tag="OO609_004287"
     CDS             <1..302
                     /locus_tag="OO609_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6635433.1"
                     /translation="DNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            305..>435
                     /locus_tag="OO609_004288"
     CDS             305..>435
                     /locus_tag="OO609_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="TRAP family transporter; with YiaMO is involved in
                     the uptake of L-dehydroascorbate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dehydroascorbate transporter large permease
                     subunit"
                     /protein_id="HCR6635434.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     "
BASE COUNT           79 a          114 c          110 g          132 t
ORIGIN      
        1 tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc gcatggcaac
       61 gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca ctggcctggg
      121 gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc ggtttgccca
      181 tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc tttgaactgc
      241 gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg caaggagctt
      301 aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta tcggtttgcc
      361 tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg acatgtttga
      421 tgtccagatc atggc
//
LOCUS       DAJTYH010000353          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTYH010000353 DAJTYH010000000
VERSION     DAJTYH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            35..434
                     /locus_tag="OO609_004289"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO609_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTYH010000354          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTYH010000354 DAJTYH010000000
VERSION     DAJTYH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..184
                     /locus_tag="OO609_004290"
     CDS             <1..184
                     /locus_tag="OO609_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6635435.1"
                     /translation="RDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYL
                     RQRACNGLSDNRCLEI"
     gene            314..>434
                     /locus_tag="OO609_004291"
     CDS             314..>434
                     /locus_tag="OO609_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR6635436.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQ"
BASE COUNT          119 a           98 c          126 g           91 t
ORIGIN      
        1 gcgtgactac atcagtatca tgcccaaacc agacgggtta acggcagcaa agaaccttgc
       61 agaggcgttc gagcattata acgaatggca tccgcatagt gcgctgggtt atcgctcgcc
      121 acgggaatat ctgcggcagc gggcttgtaa tgggttaagt gataacagat gtctggaaat
      181 ataggggcaa atccaacgga ttcctcgatg aaattattgc gtgttgcaga ggggatgtga
      241 cggctgcaaa caaaaccaat cccgtgagcc tgctactatt gatggattca tgccaaaagc
      301 acaggagagc attatggggc aacaaaagca gcgtaatcga cgttgggttc tggcctcgcg
      361 tccacatggc gcaccggttc cggagaattt ccgtcttgaa gaagatgatg tcgccacacc
      421 gggtgaagga cagg
//
LOCUS       DAJTYH010000355          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTYH010000355 DAJTYH010000000
VERSION     DAJTYH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..366
                     /gene="torD"
                     /locus_tag="OO609_004292"
     CDS             <1..366
                     /gene="torD"
                     /locus_tag="OO609_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6635437.1"
                     /translation="CGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFNEP
                     ADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSFGF
                     YAALSQLLLVLVECDHQNR"
BASE COUNT          104 a           97 c          126 g          103 t
ORIGIN      
        1 tgcggcctgt ttctgatgac cgacaaacaa gcggcgctgc cgtatgcatc ggcctacaaa
       61 caggacgagc aagagattaa acgcttgtta gttgaggcag ggatggaaac cagtggcaat
      121 ttcaacgaac cggcagatca tctggcgatc tatctcgagt tgttgagtta tctgcatttt
      181 tcgctgggag aggggaccgt tcctgcgcga agaatcgaca gtttgcggca aaaaacactg
      241 acggcgctgc ggcaatggtt accagagttt gctgcgcgtt gtcgacagta tgacagcttt
      301 ggtttttacg cggcactaag ccagttattg ctggtgttag tggagtgcga ccaccaaaac
      361 agataacgtc gtttatgcgc ctgaaaagac gcgtttagcg tcgcatcagg cattatggcg
      421 cagttgccgg
//
LOCUS       DAJTYH010000356          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTYH010000356 DAJTYH010000000
VERSION     DAJTYH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            104..>424
                     /locus_tag="OO609_004293"
     CDS             104..>424
                     /locus_tag="OO609_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6635438.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLP"
BASE COUNT          157 a           70 c           71 g          126 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacgaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccct
//
LOCUS       DAJTYH010000357          422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTYH010000357 DAJTYH010000000
VERSION     DAJTYH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..311
                     /locus_tag="OO609_004294"
     CDS             <1..311
                     /locus_tag="OO609_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6635439.1"
                     /translation="NSSPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKVMNRG
                     LRKILLARHALFDEVASASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE
                     "
     gene            complement(313..422)
                     /locus_tag="OO609_004295"
                     /pseudo
     CDS             complement(313..422)
                     /locus_tag="OO609_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          107 a          110 c           84 g          121 t
ORIGIN      
        1 gtaactcttc gccattagac tctctcaatc cgcgaaaaat atttatttcc gccagcggtg
       61 tgcataatca ctttggcgtc agctggttta accctgaaga tcttgccact aagcgtaaag
      121 tgatgaaccg tggactacgg aaaattttgc tcgcccgcca cgcgttgttc gatgaagtgg
      181 cctctgccag cctcgcaccg atctctgcat ttgacgttct gattagcgat cgtccgttac
      241 cggcagatta tgttacgcac tgccagaatg gttctgtaaa gatcattaca cctgattcag
      301 aagacgaatg acttactgaa aaaacaccac aatcttgtta aacatcgtcg ggttggactg
      361 attacgttgc actttcacca catattccag cttatctatt tggcttatca cctactccag
      421 ac
//
LOCUS       DAJTYH010000358          419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTYH010000358 DAJTYH010000000
VERSION     DAJTYH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..362
                     /locus_tag="OO609_004296"
     CDS             <1..362
                     /locus_tag="OO609_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6635440.1"
                     /translation="NHIPDAAMTIATAALFAKGTTTLRNIYNWRVKETDRLFAMATEL
                     RKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFSLVALSDTPVTILDPKCTA
                     KTFPDYFEQLARISQAA"
BASE COUNT          114 a          103 c          106 g           96 t
ORIGIN      
        1 tgaaccatat tcctgatgcg gcgatgacca ttgccacggc ggcgttattt gcaaaaggca
       61 ccaccacgct gcgcaatatc tataactggc gtgttaaaga gaccgatcgc ctgtttgcga
      121 tggcaacaga actgcgtaaa gtcggcgcgg aagtggaaga ggggcacgat tacattcgta
      181 tcactcctcc ggaaaaactg aactttgccg agatcgcgac atacaatgat caccggatgg
      241 cgatgtgttt ctcgctggtg gcgttgtcag atacaccagt gacgattctt gatcccaaat
      301 gcacggccaa aacatttccg gattatttcg agcagctggc gcggattagc caggcagcct
      361 gaatgaacaa cgggcaataa atagccaaat ctttctttat caaaacgtcg gcacattgt
//
LOCUS       DAJTYH010000359          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTYH010000359 DAJTYH010000000
VERSION     DAJTYH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            39..>417
                     /locus_tag="OO609_004297"
     CDS             39..>417
                     /locus_tag="OO609_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6635441.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPEFPGRKSSRKPLPA"
BASE COUNT          129 a          105 c          106 g           77 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacccga
      361 tcgccagtaa gcctgaattc ccgggacgaa aatcttcacg aaaaccgctg ccggcag
//
LOCUS       DAJTYH010000360          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTYH010000360 DAJTYH010000000
VERSION     DAJTYH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="OO609_004298"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="OO609_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6635442.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAJTYH010000361          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTYH010000361 DAJTYH010000000
VERSION     DAJTYH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..142)
                     /locus_tag="OO609_004299"
     CDS             complement(<1..142)
                     /locus_tag="OO609_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6635443.1"
                     /translation="MGSTRVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSK
                     APF"
     gene            complement(310..>406)
                     /locus_tag="OO609_004300"
     CDS             complement(310..>406)
                     /locus_tag="OO609_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635444.1"
                     /translation="MKFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          127 a           85 c           69 g          125 t
ORIGIN      
        1 taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc tggatgagat
       61 aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta ttacttaagt
      121 aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt tggcctaaaa
      181 aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta aagagcctga
      241 ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa taaaaaggcc
      301 cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg ccgtaccagc
      361 cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatt
//
LOCUS       DAJTYH010000362          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTYH010000362 DAJTYH010000000
VERSION     DAJTYH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            113..>397
                     /locus_tag="OO609_004301"
     CDS             113..>397
                     /locus_tag="OO609_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6635445.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISK"
BASE COUNT          127 a           89 c           94 g           87 t
ORIGIN      
        1 tagtgaaaag ggataaaagc attgtcatct gcggcagcta tgagtaatgt tggccctaac
       61 gaatagcggt tgcttaaacg aatccgactc tcacattatc aggggtataa aaatggaaac
      121 taccaagcct tcattccagg acgtactgga atttgttcgt ctgttccgtc gtaagaacaa
      181 actgcaacgt gaaattcagg acgttgagaa aaagatccgt gacaaccaga agcgcgtcct
      241 gttgctggac aacctgagcg attacatcaa gccagggatg agcgttgaag caatccaggg
      301 catcatcgcc agcatgaaag gtgactatga agatcgcgtt gacgattaca tcatcaaaaa
      361 tgccgagctc tccaaagaac gccgcgatat ctccaaa
//
LOCUS       DAJTYH010000363          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTYH010000363 DAJTYH010000000
VERSION     DAJTYH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>391
                     /locus_tag="OO609_004302"
     CDS             <1..>391
                     /locus_tag="OO609_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6635446.1"
                     /translation="VYGKVKAMHYMSDNASKDGDQSYIRFGFKGETQINDQLTGYGRW
                     EAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGD
                     SSAQTDNFMTKRASGLATYRNTDFFGV"
BASE COUNT          103 a           78 c          108 g          102 t
ORIGIN      
        1 atgtctatgg caaagttaaa gccatgcatt atatgagtga taacgccagt aaagatggcg
       61 accagagtta tatccgtttt ggttttaaag gtgaaactca aattaacgat caactgactg
      121 gttatggtcg gtgggaagcg gagtttgccg gaaataaagc ggagagtgat actgcacagc
      181 aaaaaacgcg tctcgctttt gccgggttga aatataaaga tttgggttct ttcgattatg
      241 gtcgtaacct gggcgcgttg tatgacgtgg aagcctggac cgatatgttc ccggaatttg
      301 gtggcgattc ctcggcgcag accgacaact ttatgaccaa acgcgccagc ggtctggcga
      361 cgtatcggaa caccgacttc ttcggcgtta t
//
LOCUS       DAJTYH010000364          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTYH010000364 DAJTYH010000000
VERSION     DAJTYH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..144)
                     /locus_tag="OO609_004303"
     CDS             complement(<1..144)
                     /locus_tag="OO609_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCR6635447.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSAT"
     gene            complement(169..>391)
                     /locus_tag="OO609_004304"
     CDS             complement(169..>391)
                     /locus_tag="OO609_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HCR6635448.1"
                     /translation="AQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVI
                     VSGLQRIRPGIKARAISSSQENASTESKQ"
BASE COUNT           93 a          104 c          104 g           90 t
ORIGIN      
        1 ggtagcgctg acggtaatgg tcggtggcgc aatctgcgga tactgcgcaa ccggtaagtt
       61 catgatcgcc agaccacctg caagcatcat aataatggca agtacccagg caaaaaccgg
      121 gcgatcaata aaatagttag ccatgaaagt ccccttaagc ctgcaacgtt attgtttcga
      181 ttcggtgctg gcgttttcct ggctggagga aattgctcgt gctttgatac ccggacgaat
      241 gcgttgcaaa ccggaaacga tcacccgatc gccagcctgc aagccagagg tgacaaccca
      301 ctggtcgccg atggctttgc tggcttcaat ttcgcgtagc tgcacgacat cgtctttatc
      361 cagaatgagc gccgttgctt taccctgggc g
//
LOCUS       DAJTYH010000365          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTYH010000365 DAJTYH010000000
VERSION     DAJTYH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
BASE COUNT          106 a           69 c           76 g          127 t
ORIGIN      
        1 acattattga ttttggggtt tcagggaagt aatccatttc gactataact gacaggtcat
       61 cacgacgtat gactgcgtat ttgctactaa atagtttctt tattttttcc acgatgcccc
      121 caggtttata agtacaaacg gttatatcca catagagaca aaaatattaa tctgataaat
      181 atttatttca cgccgtatat ttgattgttt aatgtgcaag tacaatgact tttatttttt
      241 gttgtgtata taatcaaata tatggttatt tttcaccctg cgtattcagc acgcaacaaa
      301 aaacccgccg aagcgggttt agtgcgggtg cgttgaggat gcctgacaca tgagaggtgg
      361 cgagggattt ctcccccg
//
LOCUS       DAJTYH010000366          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTYH010000366 DAJTYH010000000
VERSION     DAJTYH010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            1..>375
                     /locus_tag="OO609_004305"
     CDS             1..>375
                     /locus_tag="OO609_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="YhcL; uncharacterized member of the DcuC family of
                     anaerobic dicarboxylate transporters; probable role in
                     transport of carboxylates across the inner membrane from
                     the periplasm; its encoding gene is not essential for
                     growth and is probably cryptic; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter"
                     /protein_id="HCR6635449.1"
                     /translation="MIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGV
                     LDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLLSR
                     PLKLIRSPYIILSATYVIGQIMA"
BASE COUNT           80 a           89 c          103 g          103 t
ORIGIN      
        1 ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa aaactacaaa
       61 cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt ctggttaggg
      121 ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga catttataat
      181 gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat tatggcggtg
      241 ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt gagcttgcta
      301 agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac ttacgtcatc
      361 ggccaaatca tggcg
//
LOCUS       DAJTYH010000367          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTYH010000367 DAJTYH010000000
VERSION     DAJTYH010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>374
                     /locus_tag="OO609_004306"
     CDS             <1..>374
                     /locus_tag="OO609_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6635450.1"
                     /translation="VAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTDFFG
                     MVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQSGDG
                     KGERAEVWALSTKYDANNVYAAV"
BASE COUNT           98 a           70 c           97 g          109 t
ORIGIN      
        1 gtcgcgtatg atgcagaagc atttactgat atgtttgttg agtggggcgg tgactcatgg
       61 gctggtactg acctgttcat gaccaaccgt accaacggtg ttgcaaccta tcgtaacaca
      121 gatttctttg gcatggttga aggtctaaat tttgctctgc aatatcaggg taaaaatgaa
      181 ggtacaggca actacaaagc aaatggcgat ggccatggtt tatctgcaac ttacactatt
      241 gacggtttct catttgctgg tgcttatgca aactctgacc gtactgattg gcagtctggt
      301 gatggtaaag gcgaaagagc cgaagtttgg gcgttgtcaa caaaatatga cgctaacaat
      361 gtttatgctg ctgt
//
LOCUS       DAJTYH010000368          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTYH010000368 DAJTYH010000000
VERSION     DAJTYH010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..311)
                     /locus_tag="OO609_004307"
     CDS             complement(<1..311)
                     /locus_tag="OO609_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6635451.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTYH010000369          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTYH010000369 DAJTYH010000000
VERSION     DAJTYH010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>372)
                     /locus_tag="OO609_004308"
     CDS             complement(<1..>372)
                     /locus_tag="OO609_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6635452.1"
                     /translation="DPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAFFEAYMDADYTD
                     RGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSM
                     DVDIYNLHPAHAKRVEEIIAE"
BASE COUNT           83 a          100 c           99 g           90 t
ORIGIN      
        1 ttttcagcga taatttcctc gacacgttta gcgtgtgccg gatgaaggtt ataaatgtcc
       61 acgtccattg aacgagccag tacgccagtg gctacgtcgc gcgccagtga cgtcagatcg
      121 tgaacgaaac cttcgccgcg atcggtgagg ttcccgccgc cagcattagc gccggaagcc
      181 gtgcgcgtga tgcgtgaaac acgatttcct ttcatccact cttttgtcag cagaccgcga
      241 tcggtgtagt ctgcgtccat gtatgcttcg aaaaaagcag tcatcagccc caggcttgaa
      301 ttgcctggat tagggaaaac tctgtcagta tcacgcacca gtttgtggag atcgcgaatt
      361 tccagcggat cg
//
LOCUS       DAJTYH010000370          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTYH010000370 DAJTYH010000000
VERSION     DAJTYH010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(1..371)
                     /locus_tag="OO609_004309"
                     /pseudo
     CDS             complement(1..371)
                     /locus_tag="OO609_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          107 a           90 c           66 g          108 t
ORIGIN      
        1 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
       61 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
      121 ctttatcatg catcttttct accgtacttt tcagccacta cggtacatca tcaatcacct
      181 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
      241 cttccagccc tgtttcgcgg aaatttaatg aagaaatcac tgtttccaca taggtgagaa
      301 aagcgttatt ggtccggtat acctgcgaag caacaaagca ataaggcaac aacggcaggt
      361 aatgctgctc a
//
LOCUS       DAJTYH010000371          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTYH010000371 DAJTYH010000000
VERSION     DAJTYH010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>370)
                     /locus_tag="OO609_004310"
     CDS             complement(<1..>370)
                     /locus_tag="OO609_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HCR6635453.1"
                     /translation="ASGSDIAFERLVPVLEAVAGKVYRIGAEPGLGSTVKIIHQLLAG
                     VHIAAGAEAMALAARAGIPLDVMYDVVTNAAGNSWMFENRMRHVVDGDYTPHSAVDIF
                     VKDLGLVADTAKALHFPLPLA"
BASE COUNT           91 a          108 c           94 g           77 t
ORIGIN      
        1 gccaatggca gcgggaagtg cagggctttg gctgtatcgg caaccagacc aagatcctta
       61 acaaaaatat cgacggctga atgcggggtg taatcgccat ccaccacatg acgcatccgg
      121 ttttcgaaca tccaggaatt tccggcggca ttggtcacga cgtcatacat cacatccagc
      181 gggatccccg cacgggctgc aagtgccatc gcttcggctc cggcagcaat atgtacgccc
      241 gctaacaact ggtgaataat ttttacggtc gaacctagtc ccggttctgc acctatgcga
      301 taaacttttc cggcaacggc ttccagcacg ggtaccagtc gttcaaaggc aatatcgcta
      361 ccggaggcca
//
LOCUS       DAJTYH010000372          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTYH010000372 DAJTYH010000000
VERSION     DAJTYH010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>364
                     /locus_tag="OO609_004311"
     CDS             <1..>364
                     /locus_tag="OO609_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6635454.1"
                     /translation="LSQYTSPVSDLDGVDYPKPYRGKHKILVIAADERYLPTDNGKLF
                     STGNHPIETLLPLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLF
                     RNPKKLADVVASLNADSE"
BASE COUNT           99 a           89 c           83 g           93 t
ORIGIN      
        1 cgcttagcca atatacgagt cccgtctctg atcttgatgg cgtggactat ccaaaaccgt
       61 atcgcggtaa acataaaatt ctggtgatcg ccgcggacga acgttatttg ccgaccgata
      121 acggaaaact gttctcgacc ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc
      181 tccatgctgc aggtttcgaa ttcgaagtgg cgaccatttc cggtctgatg accaagtttg
      241 aatactgggc tatgccgcac aaagatgaaa aagtaatgcc attctttgaa cagcataaat
      301 cgttattccg taatccgaag aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg
      361 aata
//
LOCUS       DAJTYH010000373          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTYH010000373 DAJTYH010000000
VERSION     DAJTYH010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..137
                     /locus_tag="OO609_004312"
     CDS             <1..137
                     /locus_tag="OO609_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6635455.1"
                     /translation="DLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK
                     "
     gene            137..>357
                     /locus_tag="OO609_004313"
     CDS             137..>357
                     /locus_tag="OO609_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6635456.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITE"
BASE COUNT          104 a           91 c           86 g           76 t
ORIGIN      
        1 gtgacttagt agatcgtcgg agagcaaaaa acggcgataa cgaatacatg gcatgggata
       61 tgactctcgg ttggatcgtc tggcagcaac gagctggtat ccatttctca acaatgtcac
      121 agcaagaggt gaaataatgg agccatacag cctcacactc gatgaggcct gtcattttct
      181 caagatatcc agaccgactg ccattaactg gatacgcaca gggcgtcttc aggcaacacg
      241 caaagatccc actaagaata aatctcctta cctcacaaca cgacaagcct gcattgcggc
      301 tcttcagtct ccgctgcata ctgtccaggt gagcgcgggt gatggcataa cagagga
//
LOCUS       DAJTYH010000374          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTYH010000374 DAJTYH010000000
VERSION     DAJTYH010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>356
                     /locus_tag="OO609_004314"
     CDS             <1..>356
                     /locus_tag="OO609_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6635457.1"
                     /translation="WTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWET
                     RGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEW
                     MPVVGYVSFSEAAHAI"
BASE COUNT           84 a           88 c          116 g           68 t
ORIGIN      
        1 ctggacgggt aagcgctggg cgtacctcgc cgttgttctc gacctgttcg caagaaaacc
       61 agtgggctgg gccatgtcgt tctcgccgga cagcaggctc accatgaaag cgctggaaat
      121 ggcatgggaa acccgtggta agcccggcgg ggtgatgttc cacagcgatc agggcagtca
      181 ttatacgagc aggcagttcc ggcagttatt gtggcgatac cagatcagac agagtatgag
      241 ccggcgcgga aactgctggg ataacagccc aatggaacgc ttcttcagga gtctgaagaa
      301 cgaatggatg ccggtggtgg gttacgtaag cttcagcgag gcagctcacg ccataa
//
LOCUS       DAJTYH010000375          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTYH010000375 DAJTYH010000000
VERSION     DAJTYH010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(150..>350)
                     /locus_tag="OO609_004315"
     CDS             complement(150..>350)
                     /locus_tag="OO609_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635458.1"
                     /translation="KLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPPSN
                     SLQLLDETFSVSGTESSATRQH"
BASE COUNT           83 a           95 c           82 g           90 t
ORIGIN      
        1 tgcctttttt tagctaaact gcccataaaa agctcttgcg aatccagcgt cccccattcc
       61 tctgacaaca gaatctgatt tgaaggataa tgagtaaaat taccgctacc ccccaaggca
      121 ttggatcctg atgtactcct gaagcattct caatgctgtc tggtcgctga tgattccgtc
      181 ccggataccg agaacgtttc gtccagcaac tggagagagt tcgacggtgg catcattgcc
      241 catgccggag gcgctggagg tttcggctga ggatggcaca gggcattttc ctttgacgag
      301 cacccgacca ccattatcaa gcttgcgccg aagagcatca ttttcagctt
//
LOCUS       DAJTYH010000376          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTYH010000376 DAJTYH010000000
VERSION     DAJTYH010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            62..349
                     /gene="relE"
                     /locus_tag="OO609_004316"
     CDS             62..349
                     /gene="relE"
                     /locus_tag="OO609_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6635459.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT           96 a           65 c           99 g           89 t
ORIGIN      
        1 agatagtgaa agaacggctt cgtaatccta agccagtacg tgtgacgctg gatgaactct
       61 gatggcgtat tttctggatt ttgacgagcg ggcactaaag gaatggcgaa agctgggctc
      121 gacggtacgt gaacagttga aaaagaagct ggttgaagta cttgagtcac cccggattga
      181 agcaaacaag ctccgtggta tgcctgattg ttacaagatt aagctccggt cttcaggcta
      241 tcgccttgta taccaggtta tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa
      301 aagagaacgc tcggaagtat atagcgaggc ggtcaaacgc attctctga
//
LOCUS       DAJTYH010000377          348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTYH010000377 DAJTYH010000000
VERSION     DAJTYH010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
BASE COUNT          129 a           48 c           68 g          103 t
ORIGIN      
        1 caaatacaat aatctgcttg gaagatagat tatcccaacg ctttactctg taaatcaaaa
       61 gtaaaagaga atttaagggc aatatttgcc cattaaacga catctgactt cttccggggc
      121 gcgcaatata tgtaggttga actgaaaaaa tcaatacttt tagttaatca tggcgataat
      181 ttctttttca tagcgataaa atacggttta tgaaaatagg gaaacgtttg tgttgcaaca
      241 taaattgtat gacatattaa ctgtgatgat atttgtcgcg tgagggtggt aagaaacatt
      301 catataacaa atgaaatgag ttgatgagta agtgaccaaa aacgagaa
//
LOCUS       DAJTYH010000378          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTYH010000378 DAJTYH010000000
VERSION     DAJTYH010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..102)
                     /locus_tag="OO609_004317"
     CDS             complement(<1..102)
                     /locus_tag="OO609_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6635460.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKG"
BASE COUNT          138 a           49 c           45 g          115 t
ORIGIN      
        1 tcctttaatg gttaatgttc cggtatcaac gtctgcagca aatactccag cgctcataaa
       61 aaaagaagaa aagacagcgg cagcaataat tgaacgtttc ataatatgtt ccttaataaa
      121 atttattttt ggttgttaag agtctgtatc tcttggaaac aaatttctca catcaaaaaa
      181 gctgccgcaa tgtaaacata ttgttttatt gatttaaaac aaattaacca atgcaatata
      241 aatacaacaa atacttaaga atattaaata tttatcacat ctattaatta atgtagattt
      301 atgtaaatgg tattgttgat atcaataaaa aagacgaaat catttat
//
LOCUS       DAJTYH010000379          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTYH010000379 DAJTYH010000000
VERSION     DAJTYH010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
BASE COUNT          125 a           59 c           46 g          112 t
ORIGIN      
        1 cattaccata attaacggaa taattaacta ttgcgaaaaa ttaatgtaac gcagataaac
       61 acatcccgat tgaattattc ataagactat tcacaagcat tatgaatatt atgaatgtgt
      121 tcttacaaaa taatcataat cgcatatttt ttaatgaaaa atcacctcac ctacaattaa
      181 aaacacgaca tccgcaccat aaatagactt gcaaaaaata taacatcatt gttttcaatc
      241 tgccatttat gggattgacc gttttctttt gacacggagt tcaacaatgg gtaatgcttc
      301 caacttactg atttagtgta tgatggtgat tttaaggtgc tt
//
LOCUS       DAJTYH010000380          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTYH010000380 DAJTYH010000000
VERSION     DAJTYH010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>341
                     /locus_tag="OO609_004318"
     CDS             <1..>341
                     /locus_tag="OO609_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6635461.1"
                     /translation="VDAELFLKTFAEGVAMFNARTGRETEMCGGKLSFDDVFEREYAR
                     TIVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGGSLKGAKNVYYNMALMNAGVKKVV
                     VRFDPQQLHST"
BASE COUNT           98 a           70 c           94 g           79 t
ORIGIN      
        1 ctgttgatgc agagctgttt ctgaaaacct ttgccgaagg tgtggcgatg ttcaatgcca
       61 gaacaggccg tgaaacagaa atgtgcgggg gcaaactctc gtttgatgat gttttcgagc
      121 gtgaatacgc cagaacgatt gtgcgtaagc caaccgaaga acaaaaacgg atgctgttac
      181 tgcctgccga ggcggtgaac gtttcacgca aaggcgagtt tacgcttaaa gttggcggct
      241 cccttaaagg cgcgaaaaac gtttattaca acatggcatt aatgaatgcc ggagtgaaaa
      301 aagttgtggt cagatttgat ccacagcagt tacacagcac g
//
LOCUS       DAJTYH010000381          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTYH010000381 DAJTYH010000000
VERSION     DAJTYH010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..321
                     /locus_tag="OO609_004319"
     CDS             <1..321
                     /locus_tag="OO609_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635462.1"
                     /translation="ATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKN
                     ATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSVM
                     TPTY"
BASE COUNT           96 a           78 c           77 g           90 t
ORIGIN      
        1 gcgacgaaag attcagtagt cacattgcca aatgtcagcg ccaccaagtt gcaaactaat
       61 ggagcagtct ctggcgttaa aactgatgta ccaattgcct tagaaggctg tgatgttact
      121 gtaactaaaa atgcgacgtt tacctttagt ggtacggctg acggcgtgca acctacagca
      181 tttgccaacc aggctaccac cgatgcagcg acgaatgttg ctctacaaat gtatctgcca
      241 gatggttcta catcggtaac tcccggtacc gaaacgtcga atattcagtt agcggattcg
      301 gtaatgactc caacttactg atagtgtttt atgttcagat a
//
LOCUS       DAJTYH010000382          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTYH010000382 DAJTYH010000000
VERSION     DAJTYH010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>341
                     /locus_tag="OO609_004320"
     rRNA            <1..>341
                     /locus_tag="OO609_004320"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           87 a           76 c          114 g           64 t
ORIGIN      
        1 ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa acggtagcta
       61 ataccgcata acgtcgcaag accaaagagg gggaccttcg ggcctcttgc catcggatgt
      121 gcccagatgg gattagctag taggtggggt aacggctcac ctaggcgacg atccctagct
      181 ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc ctacgggagg
      241 cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc gcgtgtatga
      301 agaaggcctt cgggttgtaa agtactttca gcggggagga a
//
LOCUS       DAJTYH010000383          333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTYH010000383 DAJTYH010000000
VERSION     DAJTYH010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.392"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>333)
                     /locus_tag="OO609_004321"
     CDS             complement(<1..>333)
                     /locus_tag="OO609_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635463.1"
                     /translation="SEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTD
                     CDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVE
                     YFAMNNWS"
BASE COUNT           84 a           79 c           85 g           85 t
ORIGIN      
        1 aaggaccagt tattcatcgc aaagtattca acattaaccg gctcataaaa atagacatag
       61 cggtcattga gatcgagata atagtgaaat ttctcttcgg ccatcccaag acctgaaccg
      121 taatcatcct ggtggcggtt gccttccact gcctgatcaa aagcgggtaa tagtgctgca
      181 gcttccacaa ttgtgtcgca gtcagtggat gcggtttgta gtgtgccgcg taagcccggg
      241 tatttatggg cagtcaggtt taagccatgg gtattattgg cagactcaaa agcctcgcag
      301 atggcgcgcc gctgttctgg cgacacttcc gac
//
LOCUS       DAJTYH010000384          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTYH010000384 DAJTYH010000000
VERSION     DAJTYH010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            45..248
                     /locus_tag="OO609_004322"
     CDS             45..248
                     /locus_tag="OO609_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635464.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
BASE COUNT          100 a           75 c           91 g           65 t
ORIGIN      
        1 agctggcgca cgtcgttggt tgggaagaca ttcaggtgag taaaatgaac gagacagaat
       61 taaaacacgt tatcgctcta ctcctagaag atgcaaaacg cctccagcaa ctggagccaa
      121 atgcaggcac tggggcacgc atctggctgg ctaaagaagc gctggaatct ggcgattatg
      181 atagcgaaga agccttctac aaagcagaag gccgtgcagg atattcaccg ggtcttggcg
      241 gggtataaat accatgcgca ttgactgaat tcgcaaacaa aaacagacgc gcgatacccg
      301 gatagtcggt ctgcatagtc agatatctgg c
//
LOCUS       DAJTYH010000385          328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTYH010000385 DAJTYH010000000
VERSION     DAJTYH010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.394"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..320
                     /locus_tag="OO609_004323"
     CDS             <1..320
                     /locus_tag="OO609_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635465.1"
                     /translation="GGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQ
                     HVEIRQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVY
                     SCK"
BASE COUNT           89 a           66 c           76 g           97 t
ORIGIN      
        1 aaggtggtga ggccagagca gcttatggtg cacagttgat tgatcggcta tcgaaggatt
       61 taagtcgacg gtataaacgt ggggtctctg caaaaaactt acgccaaatg aggctttttt
      121 acctcttttt tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta acaccattgg
      181 gaattccgca gacaccatct gcagaattcc catcggcaaa gatttggcag acgctgtctg
      241 ccaaattgag aggctgtttg atatttccac agcttttgta ttgcatctcc tattttcggt
      301 ttgtatattc atgcaaataa acgaagga
//
LOCUS       DAJTYH010000386          328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTYH010000386 DAJTYH010000000
VERSION     DAJTYH010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            222..>328
                     /locus_tag="OO609_004324"
     CDS             222..>328
                     /locus_tag="OO609_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HCR6635466.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQT"
BASE COUNT          105 a           68 c           71 g           84 t
ORIGIN      
        1 acgcactttg cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc
       61 ctggtgtccc tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg
      121 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
      181 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      241 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      301 cagttgctca atgtacctat aaccagac
//
LOCUS       DAJTYH010000387          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTYH010000387 DAJTYH010000000
VERSION     DAJTYH010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.396"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            6..>315
                     /gene="tnpA"
                     /locus_tag="OO609_004325"
     CDS             6..>315
                     /gene="tnpA"
                     /locus_tag="OO609_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6635467.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     D"
BASE COUNT           93 a           69 c           67 g           86 t
ORIGIN      
        1 gtcaaatgat actttggtct gtgaaaaaag aaaccgatat ccggcgtgga aggcattgtg
       61 ttttcctgat gcatgttcac ctggtctttg tcaccaaata ccgacgccag atttttgacc
      121 atgacacgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgcagctg
      181 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac tatcctccca
      241 aactggcgat atccagtctg gtaaacagcc tcaaaggcat atcgaatagg ttactgcgac
      301 gagatcgacc agata
//
LOCUS       DAJTYH010000388          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTYH010000388 DAJTYH010000000
VERSION     DAJTYH010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.397"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>312
                     /locus_tag="OO609_004326"
     CDS             <1..>312
                     /locus_tag="OO609_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6635468.1"
                     /translation="TLTDSSLRARPLFNSYRKIIASPDYISRYGKPETIDDLKQHVCL
                     GFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLSDYMIDKEIA
                     RG"
BASE COUNT           84 a           76 c           74 g           78 t
ORIGIN      
        1 tacgttaacg gactccagct tacgtgccag gccgttattt aacagttatc gaaaaattat
       61 cgcctccccc gattatattt cccgctacgg gaagccagaa acgatcgacg atttaaagca
      121 acatgtttgc ctgggattca ctgaacccgc ttccctcaat acctggccga tagcctgtag
      181 tgatggacaa ttacatgagg tgaagtacgg tttgtcatcc aatagtgggg aaacactgaa
      241 acagctttgc ctgagtggca acgggattgc gtgtttgtcc gactacatga tcgacaaaga
      301 aatcgctcgc gg
//
LOCUS       DAJTYH010000389          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTYH010000389 DAJTYH010000000
VERSION     DAJTYH010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.398"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..>310
                     /locus_tag="OO609_004327"
     CDS             <1..>310
                     /locus_tag="OO609_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR6635469.1"
                     /translation="VALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKV
                     GMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGCPVPIVI
                     A"
BASE COUNT           74 a           65 c           93 g           78 t
ORIGIN      
        1 cgtggcgtta tcgatggatg atgccgtgcg cctgaacagt tgcgcggtgg cggcgcaggt
       61 ttatatcggc agcgaatatg aacatcagtc gatcaaaaat attattcagc tggtcgatgc
      121 cggaatgaaa gtgggaatgc cgaccatggc cgtgactggc gtgggcaaag atatggtgcg
      181 cgatcagcgt tatttctcgc tcgcgactcg aatcgccgct gaaatggggg cgcaaattat
      241 caaaacctat tatgtcgaaa aaggttttga acggattgtt gccggatgtc cggtacccat
      301 tgttattgct
//
LOCUS       DAJTYH010000390          306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTYH010000390 DAJTYH010000000
VERSION     DAJTYH010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.399"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..285
                     /locus_tag="OO609_004328"
     CDS             <1..285
                     /locus_tag="OO609_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6635470.1"
                     /translation="NTWVTVSPKLNMRGGYEVLSQALERANEIKHPVGRVRDIEALDE
                     LLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLNIA"
BASE COUNT           90 a           68 c           76 g           72 t
ORIGIN      
        1 aatacctggg ttaccgtatc gccaaagctg aacatgcgcg gcggctatga agtgttgtca
       61 caggcactgg agcgagccaa cgaaatcaag catccagtgg ggcgcgtacg cgatattgaa
      121 gcactggatg aactactggc aacgctgacc gatgataaac cgcgagtcat tgcactgcag
      181 ccgattagcc aaaaggatga tgccacacga ttgtgcattg aaacctgtat tgcgcgtaat
      241 tggcgtttgt cgatgcaaac acataaatat ttaaatattg cctgattaca catttataag
      301 cgttat
//
LOCUS       DAJTYH010000391          306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTYH010000391 DAJTYH010000000
VERSION     DAJTYH010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.400"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..169)
                     /locus_tag="OO609_004329"
     CDS             complement(<1..169)
                     /locus_tag="OO609_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6635471.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGK"
     gene            complement(185..>306)
                     /locus_tag="OO609_004330"
     CDS             complement(185..>306)
                     /locus_tag="OO609_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6635472.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT           76 a           75 c           60 g           95 t
ORIGIN      
        1 tttttcctat ttttgctccg aataaacgta ataaaaaagc ccgccagcga tacaatactt
       61 gtggcgacca ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt
      121 tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggttttcctt
      181 atattcagac tttgttatat aaggctttag tcttacctgt cgtacgttgg cgtaaatgcc
      241 aggataattc tgcccagaag ccaggtacat gtaatatctg ccgttggact tttttggcgt
      301 cagcac
//
LOCUS       DAJTYH010000392          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTYH010000392 DAJTYH010000000
VERSION     DAJTYH010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.401"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..>304)
                     /locus_tag="OO609_004331"
     CDS             complement(<1..>304)
                     /locus_tag="OO609_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCR6635473.1"
                     /translation="AERLEDGSYRLVGHKWFFSVPQSDAHLVLAQTAGGLSCFFVPRF
                     LPDGQRNAIRLERLKDKLGNRSNASCEVEFQDAIGWLLGQEGEGIRLILKMGGMTRF"
BASE COUNT           77 a          107 c           64 g           56 t
ORIGIN      
        1 caaaacgcgt catccccccc attttcagga tcagacgaat tccttcccct tcctgcccca
       61 ataaccagcc aatggcatcc tgaaactcca cttcgcaact ggcgttagag cgattaccca
      121 gcttatcttt cagccgctcg aggcgaatcg cgttgcgttg cccgtcaggc aaaaagcgcg
      181 gcacaaaaaa gcaggacaga ccgcccgcgg tctgcgccag caccagatgc gcatcgcttt
      241 gcggcaccga gaaaaaccat ttatgcccca ccagccgata agagccatct tccaaacgct
      301 ctgc
//
LOCUS       DAJTYH010000393          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTYH010000393 DAJTYH010000000
VERSION     DAJTYH010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.402"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..130)
                     /locus_tag="OO609_004332"
     CDS             complement(<1..130)
                     /locus_tag="OO609_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6635474.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
     gene            complement(141..>303)
                     /locus_tag="OO609_004333"
     CDS             complement(141..>303)
                     /locus_tag="OO609_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635475.1"
                     /translation="VGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSFSTPESESG
                     LIEQGVTCH"
BASE COUNT           93 a           69 c           57 g           84 t
ORIGIN      
        1 ccgagaaatc aagcgtactg tgaaatacgc cattatcagg ggtacagata cctttaggag
       61 ctgcaagtgc tacactactt aacgagagca taaacacagc aaagcaccac tgtttgatat
      121 acttgttcat aaaaatcctt ttaatgacat gtcacaccct gttcaattaa tccgctctca
      181 ctttctggtg tcgaaaatga ataattgaag atgcattttg ttgaggcgtt ttctccccag
      241 gataacgtaa cctgtccttt ttctggtaga ccagaaagat aaagttcgcc acggtcaccg
      301 aca
//
LOCUS       DAJTYH010000394          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTYH010000394 DAJTYH010000000
VERSION     DAJTYH010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.403"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            153..>303
                     /locus_tag="OO609_004334"
     CDS             153..>303
                     /locus_tag="OO609_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6635476.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTL"
BASE COUNT           90 a           85 c           67 g           61 t
ORIGIN      
        1 gctgcatccg actcttttat caacgcttac gtaattaagg gacacagcct aaatagctgc
       61 gcggaatagt agatcccttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc
      121 aaatcaaaac aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg
      181 acgaacgacg cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca
      241 gactgactgc catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc
      301 tct
//
LOCUS       DAJTYH010000395          298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTYH010000395 DAJTYH010000000
VERSION     DAJTYH010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.denovo.404"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            19..291
                     /locus_tag="OO609_004335"
                     /pseudo
     CDS             19..291
                     /locus_tag="OO609_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           65 a           88 c           71 g           74 t
ORIGIN      
        1 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
       61 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
      121 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
      181 aaaatcattg atatggccat gaatggcgtt ggatgccggg caaccgcccg cattatgggc
      241 gttggcctca acacgatttt ccgccattta aaaaactcag gccgcagtcg gtaacctc
//
LOCUS       DAJTYH010000396         5761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.174, whole genome shotgun sequence.
ACCESSION   DAJTYH010000396 DAJTYH010000000
VERSION     DAJTYH010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.174"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(105..1082)
                     /gene="intI2"
                     /locus_tag="OO609_004336"
                     /pseudo
     CDS             complement(105..1082)
                     /gene="intI2"
                     /locus_tag="OO609_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1414..1887
                     /locus_tag="OO609_004337"
     CDS             1414..1887
                     /locus_tag="OO609_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6635477.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1982..2506
                     /gene="sat2"
                     /locus_tag="OO609_004338"
     CDS             1982..2506
                     /gene="sat2"
                     /locus_tag="OO609_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6635478.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2564..3355
                     /gene="aadA1"
                     /locus_tag="OO609_004339"
     CDS             2564..3355
                     /gene="aadA1"
                     /locus_tag="OO609_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6635479.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            3431..3928
                     /locus_tag="OO609_004340"
     CDS             3431..3928
                     /locus_tag="OO609_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635480.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3989..4360
                     /locus_tag="OO609_004341"
     CDS             3989..4360
                     /locus_tag="OO609_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635481.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4770..5147
                     /locus_tag="OO609_004342"
     CDS             4770..5147
                     /locus_tag="OO609_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635482.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5170..5322
                     /locus_tag="OO609_004343"
                     /pseudo
     CDS             5170..5322
                     /locus_tag="OO609_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5378..>5761)
                     /locus_tag="OO609_004344"
     CDS             complement(5378..>5761)
                     /locus_tag="OO609_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6635483.1"
                     /translation="TLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLEVDASD
                     GVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPKG
                     LGELQVSREDMEGWAERVVREQFTH"
BASE COUNT         1565 a         1349 c         1358 g         1489 t
ORIGIN      
        1 atggtagaac tggctcatat ttatcccctc tatgattgtt tatctttttc accataaaaa
       61 gtatcagggg ctactcaggg ctacataaag atatttatct acttttactg attgataagt
      121 agcatcagtc catccgcagg actggtggtg ccggcaaaat gctgacccaa cacatgcgta
      181 tagatttgcg tggtcttaac atcgttatgc cctaagagtt cttgcacagt gcgaatatca
      241 cgccccgctt gtaatagatg cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc
      301 ttgctaacga tgcctgcttt ttgtacggct gccttcaatg cctttcgcgc aacggagtca
      361 tgcagatgat ggcggcataa tttgccgtta tacgggtggt tgcagagcgt gctggaggga
      421 aagacaaaca tccacgccgc ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa
      481 ggcagcgatg gccctacgcc ttgtaagttg tcgtcttgct gaataagccg cgcttgctca
      541 atgagttatt ttattgctgg gattaggcgc gtgggcagta ggctgtttct gcttttccca
      601 cccttaccgt catgcacagt gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa
      661 cgcaagcatt cattaatgcg caaacctgca ccatacagca gcgtaaaaat aacttggttg
      721 cgagtatcca taacctgcaa aatgcgttgc acttcatttg cagagataac agagggtagc
      781 cgtctaggct tgcttgcagg gatataatca atatcgccca acggctgttg taaaaacctg
      841 ttgtacaaaa aagctagggc atttaaagcg attttctgcg tgtttatggc tacatgtctg
      901 ctgtttgcta agctggataa aaacagcctg acctcttcac tgcccatggt ctgaggatga
      961 cgttttttgt gaaacagaat aaaacgctta atccagtgca ggtaagtttt ttcagttttc
     1021 agcgcataac ctttttgtcg catatccgtg cgtatagaat ttaaaaatgg actgttagac
     1081 ataaaacgct ccttgtcttg caactgtctg cctatacagc ctattctagc tgggatttaa
     1141 aaaagtgcct gttttttacg cctagagatg cttgtttacc ggtagagttt taatttaatg
     1201 ctaaataaat taaaatgtta tgagttcttt gggtgagata atgtgcatcg tgcaagcagg
     1261 atagacggca tgcacgattt gtaataacag agtgtcttgt atttttaaag aaagtctatt
     1321 taatacaagt gattatatta attaacggta agcatcagcg ggtgacaaaa cgagcatgct
     1381 tactaataaa atgttaacct ctgaggaaga attgtgaaac tatcactaat ggtagctata
     1441 tcgaagaatg gagttatcgg gaatggccct gatattccat ggagtgccaa aggtgaacag
     1501 ctcctgttta aagctattac ctataaccaa tggctgttgg ttggacgcaa gacttttgaa
     1561 tcaatgggag cattacccaa ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct
     1621 gacaatgaga acgtattgat ctttccatca attaaagatg ctttaaccaa cctaaagaaa
     1681 ataacggatc atgtcattgt ttcaggtggt ggggagatat acaaaagcct gatcgatcaa
     1741 gtagatacac tacatatatc tacaatagac atcgagccgg aaggtgatgt ttactttcct
     1801 gaaatcccca gcaattttag gccagttttt acccaagact tcgcctctaa cataaattat
     1861 agttaccaaa tctggcaaaa gggttaacaa gtggcagcaa cggattcgca aacctgtcac
     1921 gccttttgta ccaaaagccg cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca
     1981 tatgaagatt tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg agaaccattt
     2041 cattgttcgt gaagtgttcg atgtgcacct atccgaccaa ggctttgaac tatctaccag
     2101 aagtgtgagc ccctaccgga aggattacat ctcggatgat gactctgatg aagactctgc
     2161 ttgctatggc gcattcatcg accaagagct tgtcgggaag attgaactca actcaacatg
     2221 gaacgatcta gcctctatcg aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt
     2281 cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc tccttggcat
     2341 acgattagag acacaaacga acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt
     2401 tactctcggc ggcattgacc tgttcacgta taaaactaga cctcaagtct cgaacgaaac
     2461 agcgatgtac tggtactggt tctcgggagc acaggatgac gcctaacaat tcattcaagc
     2521 cgacaccgct tcgcggcgcg gcttaattca ggagttaaac atcatgaggg aagcggtgat
     2581 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2641 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2701 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2761 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2821 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2881 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2941 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3001 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3061 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3121 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3181 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3241 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3301 ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     3361 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagagagct
     3421 ggggaagact atgcgcgatc tgttgaaggt ggttctaagc ctcgtacttg cgatggcatc
     3481 ggggcaggca cttgctgacc tgccaattgt tttagtggat gaagctcgtc ttccctatga
     3541 ctactcccca tccaactacg acatttctcc aagcaactac gacaactcca taagcaatta
     3601 cgacaatagt ccatcaaatt acgacaactc tgagagcaac tacgataata gttcatccaa
     3661 ttacgacaat agtcgcaacg gaaatcgtag gcttatatat agcgcaaatg ggtctcgcac
     3721 tttcgccggc tactacgtca ttgccaacaa tgggacaacg aacttctttt ccacatctgg
     3781 caaaaggatg ttctacaccc caaaaggggg gcgcggcgtc tatggcggca aagatgggag
     3841 cttctgcggg gcattggtcg tcataaatgg ccaattttcg cttgccctga cagataacgg
     3901 cctgaagatc atgtatctaa gcaactagcc tgctctctaa taaaatgtta ggcctcaaca
     3961 tctagtcgca agctgagggg aaccactagt gtcatacgaa cctccaagag acggttacac
     4021 aaacgggtac attgttgatg tcatgtatga caatcgccca agtaagtatc cagctgtgtt
     4081 cagaacgtac gtccgaattc cagactatgt cgaactagat aacgcggagg agctggccga
     4141 cgcaatcatt cgagaggttg agttaaagga ggcatggctc acaagggtct tggggaactc
     4201 agccgatcct gacgcttctg tgggcaagtg ggaccggcgc ctagcgcagt gcggtggtat
     4261 tgatgagatg tcaagtgggg tgcaggcaac atacttcagt ccaaacggtc agctggtcgg
     4321 cttagtggca tacattgatg ccgaatggat ggatgaatag ctctgacgtt acgattcagg
     4381 tgagacaacc agatgggcat tgcgtacaag ctcagttgcc accgatgtgg ctacgagtct
     4441 gatcttcttt acctaggcca aggtatggcg atgcttcctg agcaaatagt cggaacgtgc
     4501 acatgctgcg acaatctaac gacgatttct ataaatgaaa gctacggagt atgctcactt
     4561 tgtggggcta aggatcgtgt gatatttaca agctatcagc atgaggcaag gccaagaaat
     4621 ccctgctatc tagagagaag tttcaaatat caatgcccaa gatgccatgc cttcagtatg
     4681 gagcctccgg atctaccgga gatgctttgg gactaatgct ccgctttgcc tcgggtatcc
     4741 gttcgaccta tgtttaagag gaaacgcacg tgtctgccgc caaagctctt gcctatgcac
     4801 tttgtgacaa gttggaaagc aaaaaagttg atgaagtcgt acgaaatctt gtttctagcg
     4861 gctggaatat tagggaagtg gcctgggaag cttcgtcctt tgatcatgaa atgccctact
     4921 ttgacacggg gcgggacttc gatattggat ttcctgacac gctgtcatcc aagattgagt
     4981 ttttgaccgc gaatggatca atcgtccaag cacgtgctcg cctgcacttc aagcaggctc
     5041 taatttttgc caaggcgtcc aaacatgtcg ccgcgctcaa gaactcattg gattactact
     5101 atggatcgtc tgtaatcccc cctcaaaacc acggaggcta tttgtagaaa attccattaa
     5161 tatgaactca aggtgttccc tacggacgaa tcagcgatga aggtggtgta cctggctatc
     5221 caggcggcga ccaagaaatg gacgatgccg atccgcaact ggaagccggc catgaaccgt
     5281 tttatgatcg aatttggtga ccgtttaaat ggccaccttt aactgaccgg caaaagcact
     5341 tacacaaaag ggtttacagg ctctttcagt cattccttta atgcgtaaat tgctctctaa
     5401 ccactctctc agcccaccct tccatatcct ctctcgatac ttgcagctcc cccaacccct
     5461 ttggatgatt cacccctcta tgcccgtctt gtccatataa ttgatcaaac aaactatttg
     5521 gccaattcaa tgagctcttc accactccac gccgtatctt ttcaaaatca ttccgccatg
     5581 tttcgctatc aacgccactc aacactttgg tagaaagcat tttcacccca tccgatgcat
     5641 ccacctctag caatacaaat tcactgccat tacgttgcac cccaacggct ttaatcactc
     5701 ttggtgagcc atctttacac agatgtaatc ggctacgccc aacttttggc aaaaccaagg
     5761 t
//
LOCUS       DAJTYH010000397         5758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.175, whole genome shotgun sequence.
ACCESSION   DAJTYH010000397 DAJTYH010000000
VERSION     DAJTYH010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.175"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(105..1082)
                     /gene="intI2"
                     /locus_tag="OO609_004345"
                     /pseudo
     CDS             complement(105..1082)
                     /gene="intI2"
                     /locus_tag="OO609_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1414..1887
                     /locus_tag="OO609_004346"
     CDS             1414..1887
                     /locus_tag="OO609_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6635484.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1982..2506
                     /gene="sat2"
                     /locus_tag="OO609_004347"
     CDS             1982..2506
                     /gene="sat2"
                     /locus_tag="OO609_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6635485.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2564..3352
                     /gene="aadA1"
                     /locus_tag="OO609_004348"
     CDS             2564..3352
                     /gene="aadA1"
                     /locus_tag="OO609_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6635486.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            3428..3925
                     /locus_tag="OO609_004349"
     CDS             3428..3925
                     /locus_tag="OO609_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635487.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3986..4357
                     /locus_tag="OO609_004350"
     CDS             3986..4357
                     /locus_tag="OO609_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635488.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4767..5144
                     /locus_tag="OO609_004351"
     CDS             4767..5144
                     /locus_tag="OO609_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635489.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5167..5319
                     /locus_tag="OO609_004352"
                     /pseudo
     CDS             5167..5319
                     /locus_tag="OO609_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5375..>5758)
                     /locus_tag="OO609_004353"
     CDS             complement(5375..>5758)
                     /locus_tag="OO609_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6635490.1"
                     /translation="TLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLEVDASD
                     GVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPKG
                     LGELQVSREDMEGWAERVVREQFTH"
BASE COUNT         1562 a         1349 c         1358 g         1489 t
ORIGIN      
        1 atggtagaac tggctcatat ttatcccctc tatgattgtt tatctttttc accataaaaa
       61 gtatcagggg ctactcaggg ctacataaag atatttatct acttttactg attgataagt
      121 agcatcagtc catccgcagg actggtggtg ccggcaaaat gctgacccaa cacatgcgta
      181 tagatttgcg tggtcttaac atcgttatgc cctaagagtt cttgcacagt gcgaatatca
      241 cgccccgctt gtaatagatg cgtagcaaac gagtgacgaa atgtatgaca agtgacacgc
      301 ttgctaacga tgcctgcttt ttgtacggct gccttcaatg cctttcgcgc aacggagtca
      361 tgcagatgat ggcggcataa tttgccgtta tacgggtggt tgcagagcgt gctggaggga
      421 aagacaaaca tccacgccgc ttgtcgataa gcagaagggt atttgtgatc taaagcaaaa
      481 ggcagcgatg gccctacgcc ttgtaagttg tcgtcttgct gaataagccg cgcttgctca
      541 atgagttatt ttattgctgg gattaggcgc gtgggcagta ggctgtttct gcttttccca
      601 cccttaccgt catgcacagt gatgcagcca ttatcaaaat caaaatcttt aacccgcaaa
      661 cgcaagcatt cattaatgcg caaacctgca ccatacagca gcgtaaaaat aacttggttg
      721 cgagtatcca taacctgcaa aatgcgttgc acttcatttg cagagataac agagggtagc
      781 cgtctaggct tgcttgcagg gatataatca atatcgccca acggctgttg taaaaacctg
      841 ttgtacaaaa aagctagggc atttaaagcg attttctgcg tgtttatggc tacatgtctg
      901 ctgtttgcta agctggataa aaacagcctg acctcttcac tgcccatggt ctgaggatga
      961 cgttttttgt gaaacagaat aaaacgctta atccagtgca ggtaagtttt ttcagttttc
     1021 agcgcataac ctttttgtcg catatccgtg cgtatagaat ttaaaaatgg actgttagac
     1081 ataaaacgct ccttgtcttg caactgtctg cctatacagc ctattctagc tgggatttaa
     1141 aaaagtgcct gttttttacg cctagagatg cttgtttacc ggtagagttt taatttaatg
     1201 ctaaataaat taaaatgtta tgagttcttt gggtgagata atgtgcatcg tgcaagcagg
     1261 atagacggca tgcacgattt gtaataacag agtgtcttgt atttttaaag aaagtctatt
     1321 taatacaagt gattatatta attaacggta agcatcagcg ggtgacaaaa cgagcatgct
     1381 tactaataaa atgttaacct ctgaggaaga attgtgaaac tatcactaat ggtagctata
     1441 tcgaagaatg gagttatcgg gaatggccct gatattccat ggagtgccaa aggtgaacag
     1501 ctcctgttta aagctattac ctataaccaa tggctgttgg ttggacgcaa gacttttgaa
     1561 tcaatgggag cattacccaa ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct
     1621 gacaatgaga acgtattgat ctttccatca attaaagatg ctttaaccaa cctaaagaaa
     1681 ataacggatc atgtcattgt ttcaggtggt ggggagatat acaaaagcct gatcgatcaa
     1741 gtagatacac tacatatatc tacaatagac atcgagccgg aaggtgatgt ttactttcct
     1801 gaaatcccca gcaattttag gccagttttt acccaagact tcgcctctaa cataaattat
     1861 agttaccaaa tctggcaaaa gggttaacaa gtggcagcaa cggattcgca aacctgtcac
     1921 gccttttgta ccaaaagccg cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca
     1981 tatgaagatt tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg agaaccattt
     2041 cattgttcgt gaagtgttcg atgtgcacct atccgaccaa ggctttgaac tatctaccag
     2101 aagtgtgagc ccctaccgga aggattacat ctcggatgat gactctgatg aagactctgc
     2161 ttgctatggc gcattcatcg accaagagct tgtcgggaag attgaactca actcaacatg
     2221 gaacgatcta gcctctatcg aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt
     2281 cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc tccttggcat
     2341 acgattagag acacaaacga acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt
     2401 tactctcggc ggcattgacc tgttcacgta taaaactaga cctcaagtct cgaacgaaac
     2461 agcgatgtac tggtactggt tctcgggagc acaggatgac gcctaacaat tcattcaagc
     2521 cgacaccgct tcgcggcgcg gcttaattca ggagttaaac atcatgaggg aagcggtgat
     2581 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2641 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2701 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2761 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2821 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2881 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2941 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3001 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3061 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3121 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3181 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3241 agctaggcag gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     3301 agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     3361 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gagagctggg
     3421 gaagactatg cgcgatctgt tgaaggtggt tctaagcctc gtacttgcga tggcatcggg
     3481 gcaggcactt gctgacctgc caattgtttt agtggatgaa gctcgtcttc cctatgacta
     3541 ctccccatcc aactacgaca tttctccaag caactacgac aactccataa gcaattacga
     3601 caatagtcca tcaaattacg acaactctga gagcaactac gataatagtt catccaatta
     3661 cgacaatagt cgcaacggaa atcgtaggct tatatatagc gcaaatgggt ctcgcacttt
     3721 cgccggctac tacgtcattg ccaacaatgg gacaacgaac ttcttttcca catctggcaa
     3781 aaggatgttc tacaccccaa aaggggggcg cggcgtctat ggcggcaaag atgggagctt
     3841 ctgcggggca ttggtcgtca taaatggcca attttcgctt gccctgacag ataacggcct
     3901 gaagatcatg tatctaagca actagcctgc tctctaataa aatgttaggc ctcaacatct
     3961 agtcgcaagc tgaggggaac cactagtgtc atacgaacct ccaagagacg gttacacaaa
     4021 cgggtacatt gttgatgtca tgtatgacaa tcgcccaagt aagtatccag ctgtgttcag
     4081 aacgtacgtc cgaattccag actatgtcga actagataac gcggaggagc tggccgacgc
     4141 aatcattcga gaggttgagt taaaggaggc atggctcaca agggtcttgg ggaactcagc
     4201 cgatcctgac gcttctgtgg gcaagtggga ccggcgccta gcgcagtgcg gtggtattga
     4261 tgagatgtca agtggggtgc aggcaacata cttcagtcca aacggtcagc tggtcggctt
     4321 agtggcatac attgatgccg aatggatgga tgaatagctc tgacgttacg attcaggtga
     4381 gacaaccaga tgggcattgc gtacaagctc agttgccacc gatgtggcta cgagtctgat
     4441 cttctttacc taggccaagg tatggcgatg cttcctgagc aaatagtcgg aacgtgcaca
     4501 tgctgcgaca atctaacgac gatttctata aatgaaagct acggagtatg ctcactttgt
     4561 ggggctaagg atcgtgtgat atttacaagc tatcagcatg aggcaaggcc aagaaatccc
     4621 tgctatctag agagaagttt caaatatcaa tgcccaagat gccatgcctt cagtatggag
     4681 cctccggatc taccggagat gctttgggac taatgctccg ctttgcctcg ggtatccgtt
     4741 cgacctatgt ttaagaggaa acgcacgtgt ctgccgccaa agctcttgcc tatgcacttt
     4801 gtgacaagtt ggaaagcaaa aaagttgatg aagtcgtacg aaatcttgtt tctagcggct
     4861 ggaatattag ggaagtggcc tgggaagctt cgtcctttga tcatgaaatg ccctactttg
     4921 acacggggcg ggacttcgat attggatttc ctgacacgct gtcatccaag attgagtttt
     4981 tgaccgcgaa tggatcaatc gtccaagcac gtgctcgcct gcacttcaag caggctctaa
     5041 tttttgccaa ggcgtccaaa catgtcgccg cgctcaagaa ctcattggat tactactatg
     5101 gatcgtctgt aatcccccct caaaaccacg gaggctattt gtagaaaatt ccattaatat
     5161 gaactcaagg tgttccctac ggacgaatca gcgatgaagg tggtgtacct ggctatccag
     5221 gcggcgacca agaaatggac gatgccgatc cgcaactgga agccggccat gaaccgtttt
     5281 atgatcgaat ttggtgaccg tttaaatggc cacctttaac tgaccggcaa aagcacttac
     5341 acaaaagggt ttacaggctc tttcagtcat tcctttaatg cgtaaattgc tctctaacca
     5401 ctctctcagc ccacccttcc atatcctctc tcgatacttg cagctccccc aacccctttg
     5461 gatgattcac ccctctatgc ccgtcttgtc catataattg atcaaacaaa ctatttggcc
     5521 aattcaatga gctcttcacc actccacgcc gtatcttttc aaaatcattc cgccatgttt
     5581 cgctatcaac gccactcaac actttggtag aaagcatttt caccccatcc gatgcatcca
     5641 cctctagcaa tacaaattca ctgccattac gttgcacccc aacggcttta atcactcttg
     5701 gtgagccatc tttacacaga tgtaatcggc tacgcccaac ttttggcaaa accaaggt
//
LOCUS       DAJTYH010000398         5104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.187, whole genome shotgun sequence.
ACCESSION   DAJTYH010000398 DAJTYH010000000
VERSION     DAJTYH010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.187"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..341
                     /gene="frdC"
                     /locus_tag="OO609_004354"
     CDS             <1..341
                     /gene="frdC"
                     /locus_tag="OO609_004354"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6635491.1"
                     /translation="LPFYRFYMLREGTAVPAVWFSIELIFALFALKNGPEAWAGFVDF
                     LQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDEKMGPEPIIKSLWAVTVVAT
                     IVILFVALYW"
     gene            352..711
                     /gene="frdD"
                     /locus_tag="OO609_004355"
     CDS             352..711
                     /gene="frdD"
                     /locus_tag="OO609_004355"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6635492.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            774..1907
                     /locus_tag="OO609_004356"
     CDS             774..1907
                     /locus_tag="OO609_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6635493.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1996..2529
                     /gene="blc"
                     /locus_tag="OO609_004357"
     CDS             1996..2529
                     /gene="blc"
                     /locus_tag="OO609_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6635494.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2526..2843)
                     /gene="sugE"
                     /locus_tag="OO609_004358"
     CDS             complement(2526..2843)
                     /gene="sugE"
                     /locus_tag="OO609_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6635495.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3019..3165)
                     /gene="ecnB"
                     /locus_tag="OO609_004359"
     CDS             complement(3019..3165)
                     /gene="ecnB"
                     /locus_tag="OO609_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6635496.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3276..3401)
                     /gene="ecnA"
                     /locus_tag="OO609_004360"
     CDS             complement(3276..3401)
                     /gene="ecnA"
                     /locus_tag="OO609_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6635497.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(3453..4019)
                     /gene="efp"
                     /locus_tag="OO609_004361"
     CDS             complement(3453..4019)
                     /gene="efp"
                     /locus_tag="OO609_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6635498.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            4061..5089
                     /gene="epmB"
                     /locus_tag="OO609_004362"
     CDS             4061..5089
                     /gene="epmB"
                     /locus_tag="OO609_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6635499.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1259 a         1304 c         1306 g         1235 t
ORIGIN      
        1 aattgccgtt ttatcgcttt tacatgctgc gcgaaggcac ggcggttccg gctgtgtggt
       61 tcagcattga actgattttc gcgctgtttg ccctgaaaaa tggcccggaa gcctgggcgg
      121 gattcgtcga ctttttacaa aacccggtta tcgtgatcat taacctgatc actctgacgg
      181 cagctctgct gcacaccaaa acctggtttg aactggcacc gaaagcggcc aatatcattg
      241 taaaagacga aaaaatggga ccagagccaa ttatcaaaag tctctgggcg gtaactgtgg
      301 ttgccaccat cgtaatcctg tttgttgccc tgtactggta aggagcctga gatgattaat
      361 ccaaatccaa agcgttctga cgaaccggta ttctggggcc tcttcggggc cggtggtatg
      421 tggagcgcaa ttattgcgcc ggtgatgatc ctgctggtgg gtattctgct gccactgggg
      481 ctgtttccgg gtgatgcgct gagctacgag cgcgttctgg cgttcgcgca gagcttcatt
      541 ggtcgcgtat tcctgttcct gatgatcgtt ctgccgctgt ggtgtggttt acaccgtatg
      601 caccacgcga tgcacgatct gaaaatccac gtgcctgcgg gcaaatgggt tttctacggt
      661 ctggctgcta tcctgacagt tgtcacgctg attggtgtcg ttacaatcta acgcatcgcc
      721 aatgtaaatc cggcccgcct atggcgggcc gttttgtatg gaaaccagac cttatgttca
      781 aaacgacgct ctgcgcctta ttaattaccg cctcttactc cacatttgct gcccctcaac
      841 aaatcaacga tattgtgcat cgcacaatta ccccgcttat agagcaacaa aagatccccg
      901 gtatggcggt ggcggtaatt tatcagggta aaccttatta ctttacctgg ggctatgcgg
      961 acatcgccaa aaagcagccc gtcacacagc aaacgttgtt tgagttaggt tcggtcagca
     1021 aaacatttac gggcgtgctt ggtggcgacg ctattgctcg aggggaaatc aagttaagcg
     1081 atcccgcaac aaaatactgg cctgaactta ccgctaaaca gtggaatggg atcacactat
     1141 tacatcttgc gacctacacc gctggcggcc tgccattgca ggtgccggat gaagtgaaat
     1201 cctcaagcga cttgctgcgc ttctatcaaa actggcagcc tgcatgggct ccaggaacac
     1261 aacgtctgta tgccaactcc agtatcggtt tgttcggcgc actggctgtg aagccgtctg
     1321 gtttgagttt tgagcaggcg atgcaaactc gtgtcttcca gccactcaaa ctcaaccata
     1381 cgtggattaa tgtacctccc ccagaagaaa agaattacgc ctggggatat cgcgaaggta
     1441 aggcagtgca tgtttcgcca ggggcgttag atgctgaagc ttatggtgtg aagtcgacca
     1501 ttgaagatat ggcccgctgg gtgcaaagca atttaccccc ccttgatatc aatgagaaaa
     1561 cgcttcaaca agggatacaa ctggcacaat ctcgctactg gcaaaccggc gatatgtatc
     1621 agggcctggg ctgggaaatg ctggactggc cggtcaatcc tgacagcatc attaacggca
     1681 gtgacaataa aattgcactg gcagcacgcc cggtaaaagc gattaagccc ccaactcctg
     1741 cagtacgcgc atcatgggta cataaaacgg gggcgaccgg cggatttggt agctatgtcg
     1801 cgtttattcc agaaaaagag ctgggtatcg tgatgctggc taacaaaaac tatcccaatc
     1861 cagcgagagt cgccgccgcc tgtcagattc tcaacgctct acagtaaaat ttcatcgggt
     1921 ccgaattttc ggaccttttc tgcgcttttc cttgctgtca tctacactta gaaaaaaacc
     1981 agtaaggaaa catttatgcg cctgctccct ctcgttgccg cagcgacagc tgcatttctg
     2041 gtcgttgcct gtagttttcc tacgccgccg cgtggcgtga ccgtagtgaa taatttcgac
     2101 gccaaacgtt atctcggtac ctggtatgag attgcccgtt ttgatcaccg ttttgaacgt
     2161 ggactggaaa aagtcaccgc aacatacagc ctgcgtgatg acggcggcct gaatgtcatt
     2221 aataaaggct ataaccctga cagaggaatg tggcagcaga gtgaagggaa agcgtacttt
     2281 accggcgcac caactcgcgc tgcgctgaaa gtgtcattct ttggtccttt ctatggcggt
     2341 tataacgtta ttgcactcga tcgggaatac cgccatgcgc tggtttgcgg cccggaccgc
     2401 gactacctgt ggatactctc ccgcacgcca accatttctg acgaagtgaa acaggagatg
     2461 ctggcagtcg cgacccggga agggtttgat gtcagtaagt ttatttgggt acagcagccc
     2521 ggtagttagt gagtgctgag tttcagacca ataatcccca atacgattaa cgccagactc
     2581 gccagacgca tcgggttagc ggactcaccg agcagcacaa tgccggtgat ggccgcgccg
     2641 actgcgccaa tacccgtcca cacggcataa gccgtcccta ccggtaacga tttcatcgcc
     2701 caggcaagta gcgccatact gactatcatc gccgtcacgg taataacact cggcgtcaaa
     2761 cggctaaagc cgtgggtata tttcaggcca acggcccata ccacttccag cagaccagca
     2821 ataactaaga taatccagga catatcaggc tccggaaatt tggggccgtc cccggtgaaa
     2881 agaggcgttt gcaggtcgtc ctgcaaagct gatggttgaa cggatatttt gccaggtggc
     2941 aggggaattt caactattac ctttatgacg atcgcagaaa aaacaaacgg cacgacactg
     3001 ctgtcgtacc gttattgctt attgctgcgc tttcgttgct gcgccagaaa tcgcgttacc
     3061 gccatcagaa atgtcttcac caacgccacg cgtggtgttg caggcagtta atactgttga
     3121 aagcaccaga acagaaaaga tcgctgcaat tgtcttcttc accataacgt cttcctttta
     3181 ggtaatgtta tttatgtttg cctatagcaa attaataata gacaacatcg ccaaaaatga
     3241 tggaaatctg atgatttttc ggaagagaag aaaaattagc tggcagcgcg agagatggag
     3301 ttaccgagat gtttgatgtc ttcgccgaaa ccgcaagcgg tgttacagcc cgtgagcagc
     3361 gtgctggcaa gcaaaacaag aacgataagg cgtttcatca tctctgacct gaataagtga
     3421 tggtgcagcc tgcaggccgc accacaaccg cattacttca cgcgagagac gtattcacca
     3481 gagcgggtat ccactttgat gacttcgccg atttgtacga acagcggaac tttaaccaca
     3541 gcgccagtag acagggtagc cggtttgccg ccagtacctg cggtatcacc tttcaggccc
     3601 ggatcggtat caacgatttc cagttcaacg aagttcggcg gagtaacgga gatcggctga
     3661 ccattccaca gagttacgat acactctgcc tgatccagca gccatttagc attgtcgcca
     3721 attgcttttg catcagcaga cagctgctcg aaagtttcgt tgttcatgaa gtgccagaac
     3781 tcaccgtcgt tgtacaggta agtcaggttc atatcgacaa catcagcgcc ttcagcggaa
     3841 tcagtagatt tgaaggtttt ttctacgcga gtaccggtca gcagacgacg cagtttaacg
     3901 cgagcaaatg cctggccttt acccggtttt acgaattcac tcgcttcaac cgcgtaaggt
     3961 tcgccgtcta acatgatttt aagaccagca cgaaaatcgt tgctatagta cgttgccata
     4021 aggccctctg aaatttgtta attggtagct aagccacaaa atggcgcata ttgtaaccct
     4081 aaatacccca tccagagaag attggttaac gcaacttgcc gatgttgtga ccgatcctga
     4141 tgaacttctg cgtcttttga atatagacgc ggacgaaaaa ctgttggccg gacgcagcgc
     4201 caaaaagctg tttgccctgc gtgtgccccg ctcatttatc gatcgcatgg agaaaggcaa
     4261 tccggacgat cctcttttgc gtcaggtact tacctcgcaa gatgagtttg tcgtcgcgtc
     4321 cggattctcc accgacccac tggaagaaca gcacagcgta gtgcctggtt tgttgcataa
     4381 ataccacaac cgggcgcttt tgctggtcaa aggcggctgc gcggtaaatt gccgctattg
     4441 cttccgtcgc cacttcccct atgccgaaaa tcagggcaac aagcgtaact ggcagactgc
     4501 acttgagtat gttgctgcgc atccggaact ggacgagatg attttctccg gcggcgatcc
     4561 gctgatggcg aaagagcacg agctggactg gttgctcaca caactggaag ccatcccgca
     4621 tatcaaacgt ctgcggattc acagccgtct gccgattgtg atcccggcac gtatcaccga
     4681 ggcgctggtt gaacgctttg cccgttctac gctgcaaatc ttgctggtga atcacatcaa
     4741 tcatgccaat gaggtagatg aaacgttccg tcaggcgatg gctaaattgc gccgtgtcgg
     4801 tgtcaccctg cttaaccaga gcgttctgtt acgtggtgtg aacgataacg cacaaacgtt
     4861 ggcaaacctg agtaatgcgt tgttcgatgc cggcgtgatg ccgtattacc tgcatgtgct
     4921 agataaagta cagggcgcgg cgcattttat ggtgagtgat gacgaagcac ggcagattat
     4981 gcgtgagttg ctgacattgg tgtcgggata tctggtgccg aaactggcgc gagaaattgg
     5041 cggcgaaccc agcaaaacgc cgctggatct ccagctacgc cagcagtaac tgattaaaca
     5101 ggga
//
LOCUS       DAJTYH010000399         4491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.200, whole genome shotgun sequence.
ACCESSION   DAJTYH010000399 DAJTYH010000000
VERSION     DAJTYH010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.200"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            17..1060
                     /locus_tag="OO609_004363"
     CDS             17..1060
                     /locus_tag="OO609_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6635500.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1122..1736
                     /locus_tag="OO609_004364"
                     /pseudo
     CDS             1122..1736
                     /locus_tag="OO609_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1793..3250)
                     /gene="pepD"
                     /locus_tag="OO609_004365"
     CDS             complement(1793..3250)
                     /gene="pepD"
                     /locus_tag="OO609_004365"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6635501.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3512..3970
                     /gene="gpt"
                     /locus_tag="OO609_004366"
     CDS             3512..3970
                     /gene="gpt"
                     /locus_tag="OO609_004366"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6635502.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4062..>4491
                     /locus_tag="OO609_004367"
     CDS             4062..>4491
                     /locus_tag="OO609_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6635503.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGD"
BASE COUNT         1075 a         1129 c         1201 g         1086 t
ORIGIN      
        1 atttacggaa tggtatatgg gcaattatat tcgtccctta tccgatgcgg tatttaccat
       61 cgcatctgat gacctgtgga tcgagagttt agcgatccaa caattacaca ccacggcaaa
      121 tttacccaac atgcagcgcg tagtggggat gccagattta caccccggac gcggctaccc
      181 gattggcgca gcgttcttct ccgcaggccg tttttaccca gcactggtcg gcaatgatat
      241 cggctgtggc atggcgctat ggcaaacaga tattctcgct cgcaaataca acgccgataa
      301 gtttgaaaag cgattatcag ctttggatga cgttgctgaa gaaagctggc tggaggaaaa
      361 cctgccgtca gcatttgcac agcatccgtg gcgcagctcg cttggctcta tcggtggtgg
      421 caatcatttc gctgaactgc aacaagttga tcaaatcatc aacgctgaac tgtttgcact
      481 ggcaggtctg gatgcgcagc atctgcaact gctggttcat agcggctcga gggggctagg
      541 gcagtctatt ttacagcggc atattgcctc gttttcgcat catggtttgc ctgaaggcag
      601 tgacgacgcg ctggcgtttg cgcgttttaa tcgccacctg atcgctttgc gcataatgca
      661 acaggttaag gccaccggaa gtccggttct ggatgtggcg cataactttg ttagcgcgtg
      721 tcgaatcggt gatcaacaag gggtcttaca tcgtaaaggt gccacgcctg atgactgtgg
      781 tctggtggtt attcccggtt cacgcggtga ttactccagg ctggttcagc ccgtcaggag
      841 tgaggaaaca ttgcattcgc tggcgcatgg ggctgggcgt aaatgggggc gcaccgagtg
      901 taaagggcgt ctggcagcga aatacacagc gacgcaactc tcgcgtactg aacttggcag
      961 tcgggtaatt tgtcgcgata aacaactcat cttcgaagaa gcgccacaag cctataaatc
     1021 ggctgaaagt gtggtgcaat gtcagggcag gccggattaa ttattcctgt cgcgcggctg
     1081 cgtccggtgc taacgctcaa aaacagtgga gggaaaaaag gatgatcttg ctataactct
     1141 cctcagcgca ggggccggaa gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa
     1201 ttaaagaagc tgcccgacaa gacgtcgcgg taacggtgct ggaaacagaa acaggccgct
     1261 actctgacac gctgcgttcg gcgctgattt ctctggatgg cgacaacgca tgggcgttaa
     1321 gcgaaagctg gtgtggcact attcagtgga tttgcccgag cccgtatcgc ccacatcacg
     1381 ggcgtaaaaa ctggtttctg ggcattgggc gttttaccgc tgatgagcag gagcaatccg
     1441 atgcgatccg gtatgagacg ctgcgttcgt cggggccggg cggtcaacat gtcaataaaa
     1501 ccgactcggc ggtacgcgcc acgcatttgg catccggtat tagcgtgaag gttcagtcag
     1561 agcgcagcca gcatgctaac aaacggctgg cgcgattgct gattgcctgg aagctggatc
     1621 agcagcaata ggaaaatagc gcggtgctga aatcacagcg gcgaatgttc catcaccaga
     1681 ttgaacgtgg caacccgcga cgaacgttta cagggatggc ttttatcgaa ggataatgaa
     1741 ggatgaaact gccggaaggc gattaaacgc catccggcag caaatcaaat aattacttcg
     1801 ccggaatttc tttcagcagt tcagtcagca gtgtccagta atgacctacg ctttcgatgt
     1861 gaacttgctc atccggagag tgtggaccgg tgatagttgg cccgatagaa accatgtcca
     1921 tttccggata cggttttttg aacagaccac attccaggcc cgcgtggata atctggatgt
     1981 tcggcgtctt gttgaacagg cgctgatagg tttcacgtac cagatgcatc accggagaat
     2041 tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt
     2101 tacccagcga atccagcatg ctcaccacgt agtctttacc gctgtcgatt agtgaacgga
     2161 tcaggcagtg aatttctacg ttattgtcag tcatggtcac cacaccgacg ttcagggagg
     2221 tttcgaccac accttttgcc acgtcggaat tgcggatcac accgttcggg gtggcgttca
     2281 gcagacgaat aaaggtatcg cgagatttcg caatcagggc agctttatcg ttcgctacag
     2341 agtccagcaa caaggccaga tttttctctt tctctgccag ctcgtttttc aggatctcct
     2401 gataggtatt caccagagat ttcagtgcgt cgactttatc agctgcgaca gcaatggtcg
     2461 caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca
     2521 ggtccagttc ttctgcatga cccgccagga agcgcaccag cagtttgttg gcattaccca
     2581 ggccaacgtg gatttcaccg ccggagtgac cgcctttcag accttttaag gttaacttga
     2641 aggtttcaaa accagctgga accgcttcac gatctaaatg caggttggag gtgaagtcga
     2701 taccccccgc acaacccatg tagatttcac cttcttcttc ggagtcggtg ttaatcagaa
     2761 tatcagcctg caaccagttg ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg
     2821 tcatggtcag cagcacttcc agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca
     2881 gtgcagaggc cataccaatg ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc
     2941 attcgccatc aatataaggc tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat
     3001 ttttctgcgg caccatatcg aggtgggcct gtaagacgac cggtttacga ttttccatac
     3061 ctgcggtagc aggttgacga atcaggatat tacctacctg atcgcgttcg acatggaaac
     3121 ctttctcttt tgcccaacca acaatgtatt cagcgagttg ctcttcatga taggacgggt
     3181 gaggaataga acagattttg gcaaaaatat cccacagcgg ctgtggagat aattgagaca
     3241 gttcagacac gttaagtctc cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg
     3301 ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga tatgtattga gaataccact
     3361 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     3421 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     3481 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     3541 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     3601 aggcattatt gccgtaagcc gtggcggtct ggtaccgggg gcgttactgg cgcgtgaact
     3661 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     3721 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     3781 ggtggatacc ggtggtactg ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     3841 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     3901 gcaagatacc tggatcgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     3961 cggtcgctaa tcttttcaac gcctggcatt gccgggcgtt gttcttttta acttcaggca
     4021 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     4081 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     4141 ttaatcacgg cgcacaaccg cctgtgcagt cggctcttga tggtaaaacc atccctcact
     4201 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc
     4261 tcgacgtcca ggcacgtatt gtgatgagcg atgctgaacg taccgacgat gatttatacg
     4321 ataccgtgat tggctaccgt ggtggcaact ggatttatga gtgggccacc caggcgatgg
     4381 tgtggcaaca aaaagcctgt acggaagaag atccgcaact cagtggtcga cactggctgc
     4441 atgcggctac gttgtacaac attgccgcct atcctcatct gaaaggagat g
//
LOCUS       DAJTYH010000400         3706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.211, whole genome shotgun sequence.
ACCESSION   DAJTYH010000400 DAJTYH010000000
VERSION     DAJTYH010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.211"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..396
                     /locus_tag="OO609_004368"
     CDS             <1..396
                     /locus_tag="OO609_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCR6635504.1"
                     /translation="NQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPV
                     KNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVS
                     ALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            509..1300
                     /gene="aadA1"
                     /locus_tag="OO609_004369"
     CDS             509..1300
                     /gene="aadA1"
                     /locus_tag="OO609_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6635505.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1376..1873
                     /locus_tag="OO609_004370"
     CDS             1376..1873
                     /locus_tag="OO609_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635506.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1934..2305
                     /locus_tag="OO609_004371"
     CDS             1934..2305
                     /locus_tag="OO609_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635507.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2715..3092
                     /locus_tag="OO609_004372"
     CDS             2715..3092
                     /locus_tag="OO609_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635508.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3115..3267
                     /locus_tag="OO609_004373"
                     /pseudo
     CDS             3115..3267
                     /locus_tag="OO609_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3323..>3706)
                     /locus_tag="OO609_004374"
     CDS             complement(3323..>3706)
                     /locus_tag="OO609_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6635509.1"
                     /translation="TLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLEVDASD
                     GVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPKG
                     LGELQVSREDMEGWAERVVREQFTH"
BASE COUNT         1025 a          909 c          868 g          904 t
ORIGIN      
        1 aatcaagact tctctggaga taaagaaaga aacaacggat taacagaagc atggctcgaa
       61 agtagcttaa aaatttcacc agaagaacaa attcaattcc tgcgtaaaat tattaatcac
      121 aatctcccag ttaaaaactc agccatagaa aacaccatag agaacatgta tctacaagat
      181 ctggataata gtacaaaact gtatgggaaa actggtgcag gattcacagc aaatagaacc
      241 ttacaaaacg gatggtttga agggtttatt ataagcaaat caggacataa atatgttttt
      301 gtgtccgcac ttacaggaaa cttggggtcg aatttaacat caagcataaa agccaagaaa
      361 aatgcgatca ccattctaaa cacactaaat ttataaaaaa tctaatggca aaatcgccca
      421 acccttcaat caagtcggga cggccaaaag caagcttttg gctcccctcg ctggcgctcg
      481 gcgcccctta tttcaaacgt taaacatcat gagggaagtg gtgatcgccg aagtatcgac
      541 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
      601 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      661 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      721 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      781 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      841 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      901 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      961 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     1021 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     1081 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     1141 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     1201 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca
     1261 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag
     1321 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga gagctgggga agactatgcg
     1381 cgatctgttg aaggtggttc taagcctcgt acttgcgatg gcatcggggc aggcacttgc
     1441 tgacctgcca attgttttag tggatgaagc tcgtcttccc tatgactact ccccatccaa
     1501 ctacgacatt tctccaagca actacgacaa ctccataagc aattacgaca atagtccatc
     1561 aaattacgac aactctgaga gcaactacga taatagttca tccaattacg acaatagtcg
     1621 caacggaaat cgtaggctta tatatagcgc aaatgggtct cgcactttcg ccggctacta
     1681 cgtcattgcc aacaatggga caacgaactt cttttccaca tctggcaaaa ggatgttcta
     1741 caccccaaaa ggggggcgcg gcgtctatgg cggcaaagat gggagcttct gcggggcatt
     1801 ggtcgtcata aatggccaat tttcgcttgc cctgacagat aacggcctga agatcatgta
     1861 tctaagcaac tagcctgctc tctaataaaa tgttaggcct caacatctag tcgcaagctg
     1921 aggggaacca ctagtgtcat acgaacctcc aagagacggt tacacaaacg ggtacattgt
     1981 tgatgtcatg tatgacaatc gcccaagtaa gtatccagct gtgttcagaa cgtacgtccg
     2041 aattccagac tatgtcgaac tagataacgc ggaggagctg gccgacgcaa tcattcgaga
     2101 ggttgagtta aaggaggcat ggctcacaag ggtcttgggg aactcagccg atcctgacgc
     2161 ttctgtgggc aagtgggacc ggcgcctagc gcagtgcggt ggtattgatg agatgtcaag
     2221 tggggtgcag gcaacatact tcagtccaaa cggtcagctg gtcggcttag tggcatacat
     2281 tgatgccgaa tggatggatg aatagctctg acgttacgat tcaggtgaga caaccagatg
     2341 ggcattgcgt acaagctcag ttgccaccga tgtggctacg agtctgatct tctttaccta
     2401 ggccaaggta tggcgatgct tcctgagcaa atagtcggaa cgtgcacatg ctgcgacaat
     2461 ctaacgacga tttctataaa tgaaagctac ggagtatgct cactttgtgg ggctaaggat
     2521 cgtgtgatat ttacaagcta tcagcatgag gcaaggccaa gaaatccctg ctatctagag
     2581 agaagtttca aatatcaatg cccaagatgc catgccttca gtatggagcc tccggatcta
     2641 ccggagatgc tttgggacta atgctccgct ttgcctcggg tatccgttcg acctatgttt
     2701 aagaggaaac gcacgtgtct gccgccaaag ctcttgccta tgcactttgt gacaagttgg
     2761 aaagcaaaaa agttgatgaa gtcgtacgaa atcttgtttc tagcggctgg aatattaggg
     2821 aagtggcctg ggaagcttcg tcctttgatc atgaaatgcc ctactttgac acggggcggg
     2881 acttcgatat tggatttcct gacacgctgt catccaagat tgagtttttg accgcgaatg
     2941 gatcaatcgt ccaagcacgt gctcgcctgc acttcaagca ggctctaatt tttgccaagg
     3001 cgtccaaaca tgtcgccgcg ctcaagaact cattggatta ctactatgga tcgtctgtaa
     3061 tcccccctca aaaccacgga ggctatttgt agaaaattcc attaatatga actcaaggtg
     3121 ttccctacgg acgaatcagc gatgaaggtg gtgtacctgg ctatccaggc ggcgaccaag
     3181 aaatggacga tgccgatccg caactggaag ccggccatga accgttttat gatcgaattt
     3241 ggtgaccgtt taaatggcca cctttaactg accggcaaaa gcacttacac aaaagggttt
     3301 acaggctctt tcagtcattc ctttaatgcg taaattgctc tctaaccact ctctcagccc
     3361 acccttccat atcctctctc gatacttgca gctcccccaa cccctttgga tgattcaccc
     3421 ctctatgccc gtcttgtcca tataattgat caaacaaact atttggccaa ttcaatgagc
     3481 tcttcaccac tccacgccgt atcttttcaa aatcattccg ccatgtttcg ctatcaacgc
     3541 cactcaacac tttggtagaa agcattttca ccccatccga tgcatccacc tctagcaata
     3601 caaattcact gccattacgt tgcaccccaa cggctttaat cactcttggt gagccatctt
     3661 tacacagatg taatcggcta cgcccaactt ttggcaaaac caaggt
//
LOCUS       DAJTYH010000401         3703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.212, whole genome shotgun sequence.
ACCESSION   DAJTYH010000401 DAJTYH010000000
VERSION     DAJTYH010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.212"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..396
                     /locus_tag="OO609_004375"
     CDS             <1..396
                     /locus_tag="OO609_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCR6635510.1"
                     /translation="NQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPV
                     KNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVS
                     ALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            509..1297
                     /gene="aadA1"
                     /locus_tag="OO609_004376"
     CDS             509..1297
                     /gene="aadA1"
                     /locus_tag="OO609_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6635511.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1373..1870
                     /locus_tag="OO609_004377"
     CDS             1373..1870
                     /locus_tag="OO609_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635512.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1931..2302
                     /locus_tag="OO609_004378"
     CDS             1931..2302
                     /locus_tag="OO609_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635513.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2712..3089
                     /locus_tag="OO609_004379"
     CDS             2712..3089
                     /locus_tag="OO609_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6635514.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3112..3264
                     /locus_tag="OO609_004380"
                     /pseudo
     CDS             3112..3264
                     /locus_tag="OO609_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3320..>3703)
                     /locus_tag="OO609_004381"
     CDS             complement(3320..>3703)
                     /locus_tag="OO609_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6635515.1"
                     /translation="TLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLEVDASD
                     GVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGVNHPKG
                     LGELQVSREDMEGWAERVVREQFTH"
BASE COUNT         1022 a          909 c          868 g          904 t
ORIGIN      
        1 aatcaagact tctctggaga taaagaaaga aacaacggat taacagaagc atggctcgaa
       61 agtagcttaa aaatttcacc agaagaacaa attcaattcc tgcgtaaaat tattaatcac
      121 aatctcccag ttaaaaactc agccatagaa aacaccatag agaacatgta tctacaagat
      181 ctggataata gtacaaaact gtatgggaaa actggtgcag gattcacagc aaatagaacc
      241 ttacaaaacg gatggtttga agggtttatt ataagcaaat caggacataa atatgttttt
      301 gtgtccgcac ttacaggaaa cttggggtcg aatttaacat caagcataaa agccaagaaa
      361 aatgcgatca ccattctaaa cacactaaat ttataaaaaa tctaatggca aaatcgccca
      421 acccttcaat caagtcggga cggccaaaag caagcttttg gctcccctcg ctggcgctcg
      481 gcgcccctta tttcaaacgt taaacatcat gagggaagtg gtgatcgccg aagtatcgac
      541 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
      601 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      661 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      721 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      781 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      841 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      901 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      961 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     1021 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     1081 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     1141 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta ggcaggctta
     1201 tcttggacaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgttcacta
     1261 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg
     1321 acgccgcttc gcggcgcggc ttaactcaag cgttagagag ctggggaaga ctatgcgcga
     1381 tctgttgaag gtggttctaa gcctcgtact tgcgatggca tcggggcagg cacttgctga
     1441 cctgccaatt gttttagtgg atgaagctcg tcttccctat gactactccc catccaacta
     1501 cgacatttct ccaagcaact acgacaactc cataagcaat tacgacaata gtccatcaaa
     1561 ttacgacaac tctgagagca actacgataa tagttcatcc aattacgaca atagtcgcaa
     1621 cggaaatcgt aggcttatat atagcgcaaa tgggtctcgc actttcgccg gctactacgt
     1681 cattgccaac aatgggacaa cgaacttctt ttccacatct ggcaaaagga tgttctacac
     1741 cccaaaaggg gggcgcggcg tctatggcgg caaagatggg agcttctgcg gggcattggt
     1801 cgtcataaat ggccaatttt cgcttgccct gacagataac ggcctgaaga tcatgtatct
     1861 aagcaactag cctgctctct aataaaatgt taggcctcaa catctagtcg caagctgagg
     1921 ggaaccacta gtgtcatacg aacctccaag agacggttac acaaacgggt acattgttga
     1981 tgtcatgtat gacaatcgcc caagtaagta tccagctgtg ttcagaacgt acgtccgaat
     2041 tccagactat gtcgaactag ataacgcgga ggagctggcc gacgcaatca ttcgagaggt
     2101 tgagttaaag gaggcatggc tcacaagggt cttggggaac tcagccgatc ctgacgcttc
     2161 tgtgggcaag tgggaccggc gcctagcgca gtgcggtggt attgatgaga tgtcaagtgg
     2221 ggtgcaggca acatacttca gtccaaacgg tcagctggtc ggcttagtgg catacattga
     2281 tgccgaatgg atggatgaat agctctgacg ttacgattca ggtgagacaa ccagatgggc
     2341 attgcgtaca agctcagttg ccaccgatgt ggctacgagt ctgatcttct ttacctaggc
     2401 caaggtatgg cgatgcttcc tgagcaaata gtcggaacgt gcacatgctg cgacaatcta
     2461 acgacgattt ctataaatga aagctacgga gtatgctcac tttgtggggc taaggatcgt
     2521 gtgatattta caagctatca gcatgaggca aggccaagaa atccctgcta tctagagaga
     2581 agtttcaaat atcaatgccc aagatgccat gccttcagta tggagcctcc ggatctaccg
     2641 gagatgcttt gggactaatg ctccgctttg cctcgggtat ccgttcgacc tatgtttaag
     2701 aggaaacgca cgtgtctgcc gccaaagctc ttgcctatgc actttgtgac aagttggaaa
     2761 gcaaaaaagt tgatgaagtc gtacgaaatc ttgtttctag cggctggaat attagggaag
     2821 tggcctggga agcttcgtcc tttgatcatg aaatgcccta ctttgacacg gggcgggact
     2881 tcgatattgg atttcctgac acgctgtcat ccaagattga gtttttgacc gcgaatggat
     2941 caatcgtcca agcacgtgct cgcctgcact tcaagcaggc tctaattttt gccaaggcgt
     3001 ccaaacatgt cgccgcgctc aagaactcat tggattacta ctatggatcg tctgtaatcc
     3061 cccctcaaaa ccacggaggc tatttgtaga aaattccatt aatatgaact caaggtgttc
     3121 cctacggacg aatcagcgat gaaggtggtg tacctggcta tccaggcggc gaccaagaaa
     3181 tggacgatgc cgatccgcaa ctggaagccg gccatgaacc gttttatgat cgaatttggt
     3241 gaccgtttaa atggccacct ttaactgacc ggcaaaagca cttacacaaa agggtttaca
     3301 ggctctttca gtcattcctt taatgcgtaa attgctctct aaccactctc tcagcccacc
     3361 cttccatatc ctctctcgat acttgcagct cccccaaccc ctttggatga ttcacccctc
     3421 tatgcccgtc ttgtccatat aattgatcaa acaaactatt tggccaattc aatgagctct
     3481 tcaccactcc acgccgtatc ttttcaaaat cattccgcca tgtttcgcta tcaacgccac
     3541 tcaacacttt ggtagaaagc attttcaccc catccgatgc atccacctct agcaatacaa
     3601 attcactgcc attacgttgc accccaacgg ctttaatcac tcttggtgag ccatctttac
     3661 acagatgtaa tcggctacgc ccaacttttg gcaaaaccaa ggt
//
LOCUS       DAJTYH010000402         3032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.223, whole genome shotgun sequence.
ACCESSION   DAJTYH010000402 DAJTYH010000000
VERSION     DAJTYH010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.223"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..534
                     /locus_tag="OO609_004382"
     CDS             <1..534
                     /locus_tag="OO609_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6635516.1"
                     /translation="AFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWD
                     SVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFF
                     VSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVG
                     RMLNELQQYCDEWQGGA"
     gene            complement(735..1112)
                     /locus_tag="OO609_004383"
     CDS             complement(735..1112)
                     /locus_tag="OO609_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6635517.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1179..>3032)
                     /locus_tag="OO609_004384"
     CDS             complement(1179..>3032)
                     /locus_tag="OO609_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6635518.1"
                     /translation="QPDDFDFLHRIGESYATLRRYAPEFLAVLKLRAAPAAKNVLDAI
                     EVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGD
                     IWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAVATDCEQYLHERLTLLEAQLATVN
                     RMAAANDLPDAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVDEWT
                     GFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTR
                     DETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGSSPG
                     RTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFA
                     LMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLAT
                     SIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGL
                     NKGEARNALARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHAL
                     RGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          609 a          806 c          913 g          704 t
ORIGIN      
        1 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
       61 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
      121 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
      181 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
      241 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
      301 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
      361 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
      421 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
      481 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt
      541 taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag
      601 cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca gggcagggtc
      661 gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc ggaagcagtg
      721 tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc gtggaggtaa
      781 taattgacga tatgatcatt tattctgcct cccagagcct gataaaaacg gttagcgccg
      841 gggttggatt tttcagcgtt ccagctaagg ctaaggcatt cctgttcaag cgcaagccgg
      901 gctataaagc gcattatcgc tttacccgtg cccttatttc gatcgcactg agaaacatac
      961 agctctttaa tatgcagctg accgctgtat cggggcgagg gataaagaat attgcaacat
     1021 gccaggccag taatgttatt gccgcagcgc gctctgatca ccagggtacc ggaaagccgg
     1081 ttgaataact ttttacagag ataatccttc atcaacgctt cctgaatgat accttcgcca
     1141 taataggggt cgtctcagaa aacggaaaat aaagcacgct aagccggttg cagaggccgt
     1201 agcggcctga acttccccgc gccgatcttg gcgctgctgc gccataggta atcaccggtc
     1261 aggttgatgt gctcccagcc gagtggcgac aggtactgca atagcgagtc atcgacggca
     1321 tgaccattgc cgcgcaacgc atgcgccgca cgctccaggt agaccgtgtt ccacagcacg
     1381 atggccgccg tcaccaggtt gaggccgctg gcccggtagc gctgctgctc gaaactgcgg
     1441 tcacggattt caccaaggcg gttgaagaac acggcacggg ccagcgcatt gcgcgcctcg
     1501 cccttgttca gcccggcatg cacgcggcgg cgtagctcga cgctttgcag ccagtcgagg
     1561 atgaacagcg tgcgctcgat gcggcccaac tcgcgcagcg cgacggccaa gccgttctgg
     1621 cgcgggtagc tgccgagttt cctgagcatc agcgaggccg tcaccgtgcc ctgcttgatc
     1681 gaggtggcca gccgcaggat ttcgtcccaa tgggcgcgga cgtgcttgat gttgagcgtg
     1741 ccgccgatca tcggcttgag cgcgtcatag gcggcatcgc ccttcgggat gtagagcttg
     1801 gtgtcgccca ggtcgcggat gcgcggcgcg aagcggaagc ccaagaggtg catcagggcg
     1861 aagacgtgat cggtgaagcc cgccgtgtcg gtgtagtgct cctcgatccg caggtcggat
     1921 tcgtggtaca gcaggccgtc gagcacgtag gttgagtcgc gcaggccgac attgaccacc
     1981 ttggtgtgga atggcgcgta ttggtcggag atgtgggtgt agaaagtccg tcctgggctg
     2041 ctgccatatt ttgggttgat gtgccccgtg ctctttgcct tgctagcggt tcggaaattc
     2101 tgtccgtccg atgatgatgt ggtgccatcg ccccagtgcc cggcaaaggg atgccgaaac
     2161 tgagcgttga ccagttcagc caacgctgtc gagtacgttt cgtcgcgggt atgccaggct
     2221 tgcagccaag cgagcttcgc gtaggtcgtg ccggggcagg actcggccat cttggtcagg
     2281 cccaggttga tcgcgtcggc caggatcgtg gtcaacaaca ggttcttgtc cttggccaga
     2341 tcgcccgatt tcaagtgcgt gaagtgccgg gtgaagcccg tccactcatc gacttcgagc
     2401 agcagttcgg tgatcttgac gtgcggcagg accatggctg tctggtctat cagcgcctgc
     2461 gcggtgtcgg gcaccgccgc atccagcggc gtgatcttca agcccgactc ggtgatgatg
     2521 gcatccggca ggtcgttggc tgccgccatg cggttgacgg tggcaagttg tgcttccagc
     2581 agcgtcagcc gctcatgcag atattgttcg cagtcggtgg ccacggccag cggcaattcg
     2641 ctggactgct tgaggctggt gaacttctcg ggcggtacca ggtagtcctc gaagtccttg
     2701 aactggcgtg aaccctgcac ccagatgtcg cccgagcgca gggagttctt caactcggac
     2761 agcgcgcaca gttcgtagta gcgccggtcg atgccggcgt cggtcatcac cagtttctgc
     2821 cagcgcggct tgatgaagcc ggtcggtgca tcggctggca gcttgcgggc gttgtcggtg
     2881 ttcatgccgc gcagcacctc aatggcatca agcacgtttt tggcggcggg cgcggcccgc
     2941 agcttgagca cggcaaggaa ttccggtgca tagcggcgca gggtggcgta gctctcgccg
     3001 atgcgatgca ggaaatcgaa gtcatcgggt tg
//
LOCUS       DAJTYH010000403         2229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.239, whole genome shotgun sequence.
ACCESSION   DAJTYH010000403 DAJTYH010000000
VERSION     DAJTYH010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.239"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            <1..124
                     /locus_tag="OO609_004385"
     CDS             <1..124
                     /locus_tag="OO609_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6635519.1"
                     /translation="VGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(102..725)
                     /gene="tetR(B)"
                     /locus_tag="OO609_004386"
     CDS             complement(102..725)
                     /gene="tetR(B)"
                     /locus_tag="OO609_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6635520.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            807..2012
                     /gene="tet(B)"
                     /locus_tag="OO609_004387"
     CDS             807..2012
                     /gene="tet(B)"
                     /locus_tag="OO609_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6635521.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2125..>2229)
                     /locus_tag="OO609_004388"
     CDS             complement(2125..>2229)
                     /locus_tag="OO609_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6635522.1"
                     /translation="MHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          561 a          434 c          480 g          754 t
ORIGIN      
        1 cgttggagcc gcattatttt cgctttatga atctaaaggg tggttaactc gacatcttgg
       61 ttaccgtgaa gttaccatca cggaaaaagg ttatgctgct tttaagaccc actttcacat
      121 ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct
      181 gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta
      241 gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct
      301 aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt
      361 tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta
      421 cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg
      481 ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc
      541 ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa
      601 tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg
      661 attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta
      721 gacatcatta attcctaatt tttgttgaca ctctatcatt gatagagtta ttttaccact
      781 ccctatcagt gatagagaaa agtgaaatga atagttcgac aaagatcgca ttggtaatta
      841 cgttactcga tgccatgggg attggcctta tcatgccagt cttgccaacg ttattacgtg
      901 aatttattgc ttcggaagat atcgctaacc actttggcgt attgcttgca ctttatgcgt
      961 taatgcaggt tatctttgct ccttggcttg gaaaaatgtc tgaccgattt ggtcggcgcc
     1021 cagtgctgtt gttgtcatta ataggcgcat cgctggatta cttattgctg gctttttcaa
     1081 gtgcgctttg gatgctgtat ttaggccgtt tgctttcagg gatcacagga gctactgggg
     1141 ctgtcgcggc atcggtcatt gccgatacca cctcagcttc tcaacgcgtg aagtggttcg
     1201 gttggttagg ggcaagtttt gggcttggtt taatagcggg gcctattatt ggtggttttg
     1261 caggagagat ttcaccgcat agtccctttt ttatcgctgc gttgctaaat attgtcgctt
     1321 tccttgtggt tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat acagataccg
     1381 aagtaggggt tgagacgcaa tcgaattcgg tatacatcac tttatttaaa acgatgccca
     1441 ttttgttgat tatttatttt tcagcgcaat tgataggcca aattcccgca acggtgtggg
     1501 tgctatttac cgaaaatcgt tttggatgga atagcatgat ggttggcttt tcattagcgg
     1561 gtcttggtct tttacactca gtattccaag cctttgtggc aggaagaata gccactaaat
     1621 ggggcgaaaa aacggcagta ctgctcggat ttattgcaga tagtagtgca tttgcctttt
     1681 tagcgtttat atctgaaggt tggttagttt tccctgtttt aattttattg gctggtggtg
     1741 ggatcgcttt acctgcatta cagggagtga tgtctatcca aacaaagagt catcagcaag
     1801 gtgctttaca gggattattg gtgagcctta ccaatgcaac cggtgttatt ggcccattac
     1861 tgtttgctgt tatttataat cattcactac caatttggga tggctggatt tggattattg
     1921 gtttagcgtt ttactgtatt attatcctgc tatcgatgac cttcatgtta acccctcaag
     1981 ctcaggggag taaacaggag acaagtgctt agttatttcg tcaccaaatg atgttattcc
     2041 gcgaaatata atgaccctct tgataaccca agagggcatt ttttacgata aagaagattt
     2101 agcttcaaat aaaacctatc tattttattt atctttcaag ctcaataaaa agccgcggta
     2161 aatagcaata aattggcctt ttttatcggc aagctctttt aggtttttcg catgtattgc
     2221 gatatgcat
//
LOCUS       DAJTYH010000404         1814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 601132
            SAMEA8900484-rid15816123.guided.259, whole genome shotgun sequence.
ACCESSION   DAJTYH010000404 DAJTYH010000000
VERSION     DAJTYH010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900484
            Sequence Read Archive: ERR6004849
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477763.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477763.1
            Genome Coverage       :: 26x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:46:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,390
            CDSs (total)                      :: 4,325
            Genes (coding)                    :: 3,955
            CDSs (with protein)               :: 3,955
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 170 of 370
            Pseudo Genes (incomplete)         :: 92 of 370
            Pseudo Genes (internal stop)      :: 145 of 370
            Pseudo Genes (multiple problems)  :: 36 of 370
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900484-rid15816123.guided.259"
                     /serovar="S. flexneri 2a"
                     /isolate="601132"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-12-06"
     gene            complement(<1..611)
                     /gene="intI1"
                     /locus_tag="OO609_004389"
     CDS             complement(<1..611)
                     /gene="intI1"
                     /locus_tag="OO609_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6635523.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQA"
     gene            821..1651
                     /locus_tag="OO609_004390"
     CDS             821..1651
                     /locus_tag="OO609_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6635524.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT          561 a          437 c          399 g          417 t
ORIGIN      
        1 gcctggtcct tcagccacca tgcccgtgca cgcgacagct gctcgcgcag gctgggtgcc
       61 aagctctcgg gtaacatcaa ggcccgatcc ttggagccct tgccctcccg cacgatgatc
      121 gtgccgtgat cgaaatccag atccttgacc cgcagttgca aaccctcact gatccgcatg
      181 cccgttccat acagaagctg ggcgaacaaa cgatgctcgc cttccagaaa accgaggatg
      241 cgaaccactt catccggggt cagcaccacc ggcaagcgcc gcgacggccg aggtcttccg
      301 atctcctgaa gccagggcag atccgtgcac agcaccttgc cgtagaagaa cagcaaggcc
      361 gccaatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag ccaggacaga
      421 aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg gaaacggatg
      481 aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
      541 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
      601 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
      661 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
      721 acgcagcagg gcagtcgccc taaaacaaag ttgggcgaac ccggagcctc attaattgtt
      781 agccgttaaa attaagccct ttaccaaacc aatacttatt atgaaaaaca caatacatat
      841 caacttcgct atttttttaa taattgcaaa tattatctac agcagcgcca gtgcatcaac
      901 agatatctct actgttgcat ctccattatt tgaaggaact gaaggttgtt ttttacttta
      961 cgatgcatcc acaaacgctg aaattgctca attcaataaa gcaaagtgtg caacgcaaat
     1021 ggcaccagat tcaactttca agatcgcatt atcacttatg gcatttgatg cggaaataat
     1081 agatcagaaa accatattca aatgggataa aacccccaaa ggaatggaga tctggaacag
     1141 caatcataca ccaaagacgt ggatgcaatt ttctgttgtt tgggtttcgc aagaaataac
     1201 ccaaaaaatt ggattaaata aaatcaagaa ttatctcaaa gattttgatt atggaaatca
     1261 agacttctct ggagataaag aaagaaacaa cggattaaca gaagcatggc tcgaaagtag
     1321 cttaaaaatt tcaccagaag aacaaattca attcctgcgt aaaattatta atcacaatct
     1381 cccagttaaa aactcagcca tagaaaacac catagagaac atgtatctac aagatctgga
     1441 taatagtaca aaactgtatg ggaaaactgg tgcaggattc acagcaaata gaaccttaca
     1501 aaacggatgg tttgaagggt ttattataag caaatcagga cataaatatg tttttgtgtc
     1561 cgcacttaca ggaaacttgg ggtcgaattt aacatcaagc ataaaagcca agaaaaatgc
     1621 gatcaccatt ctaaacacac taaatttata aaaaatctaa tggcaaaatc gcccaaccct
     1681 tcaatcaagt cgggacggcc aaaagcaagc ttttggctcc cctcgctggc gctcggcgcc
     1741 ccttatttca aacgttaaac atcatgaggg aagtggtgat cgccgaagta tcgactcaac
     1801 tatcagaggt agtt
//
